LOCUS       DACDHJ010000001        58597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDHJ010000001 DACDHJ010000000
VERSION     DACDHJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.001"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            27..1850
                     /locus_tag="IV223_000001"
     CDS             27..1850
                     /locus_tag="IV223_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8710316.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(1992..2177)
                     /locus_tag="IV223_000002"
     CDS             complement(1992..2177)
                     /locus_tag="IV223_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8710317.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            2388..2660
                     /locus_tag="IV223_000003"
     CDS             2388..2660
                     /locus_tag="IV223_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710318.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            2653..2916
                     /locus_tag="IV223_000004"
     CDS             2653..2916
                     /locus_tag="IV223_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8710319.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            2916..3182
                     /locus_tag="IV223_000005"
     CDS             2916..3182
                     /locus_tag="IV223_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8710320.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            3383..3871
                     /locus_tag="IV223_000006"
     CDS             3383..3871
                     /locus_tag="IV223_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8710321.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4412..5083)
                     /locus_tag="IV223_000007"
     CDS             complement(4412..5083)
                     /locus_tag="IV223_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710322.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5080..5997)
                     /locus_tag="IV223_000008"
     CDS             complement(5080..5997)
                     /locus_tag="IV223_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8710323.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(5994..6635)
                     /locus_tag="IV223_000009"
     CDS             complement(5994..6635)
                     /locus_tag="IV223_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710324.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(6639..7814)
                     /locus_tag="IV223_000010"
     CDS             complement(6639..7814)
                     /locus_tag="IV223_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8710325.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8007..9038
                     /gene="queA"
                     /locus_tag="IV223_000011"
     CDS             8007..9038
                     /gene="queA"
                     /locus_tag="IV223_000011"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8710326.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            9657..12296
                     /locus_tag="IV223_000012"
     CDS             9657..12296
                     /locus_tag="IV223_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8710327.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12464..13162)
                     /locus_tag="IV223_000013"
     CDS             complement(12464..13162)
                     /locus_tag="IV223_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8710328.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            13380..14525
                     /gene="tgt"
                     /locus_tag="IV223_000014"
     CDS             13380..14525
                     /gene="tgt"
                     /locus_tag="IV223_000014"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8710329.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            14622..15002
                     /gene="yajC"
                     /locus_tag="IV223_000015"
     CDS             14622..15002
                     /gene="yajC"
                     /locus_tag="IV223_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8710330.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            15307..17904
                     /gene="adhE"
                     /locus_tag="IV223_000016"
                     /gene_synonym="adhC"
     CDS             15307..17904
                     /gene="adhE"
                     /locus_tag="IV223_000016"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8710331.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            18133..19239
                     /locus_tag="IV223_000017"
     CDS             18133..19239
                     /locus_tag="IV223_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8710332.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            19256..20284
                     /locus_tag="IV223_000018"
     CDS             19256..20284
                     /locus_tag="IV223_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8710333.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            20290..20769
                     /locus_tag="IV223_000019"
     CDS             20290..20769
                     /locus_tag="IV223_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8710334.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            20785..22080
                     /locus_tag="IV223_000020"
     CDS             20785..22080
                     /locus_tag="IV223_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8710335.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            22091..22915
                     /locus_tag="IV223_000021"
     CDS             22091..22915
                     /locus_tag="IV223_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8710336.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            23080..23979
                     /locus_tag="IV223_000022"
     CDS             23080..23979
                     /locus_tag="IV223_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8710337.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(24105..24974)
                     /locus_tag="IV223_000023"
     CDS             complement(24105..24974)
                     /locus_tag="IV223_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8710338.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            25127..26587
                     /gene="rhaB"
                     /locus_tag="IV223_000024"
     CDS             25127..26587
                     /gene="rhaB"
                     /locus_tag="IV223_000024"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8710339.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            26584..27840
                     /gene="rhaA"
                     /locus_tag="IV223_000025"
     CDS             26584..27840
                     /gene="rhaA"
                     /locus_tag="IV223_000025"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8710340.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            27854..28699
                     /gene="rhaD"
                     /locus_tag="IV223_000026"
     CDS             27854..28699
                     /gene="rhaD"
                     /locus_tag="IV223_000026"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8710341.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            28701..29012
                     /gene="rhaM"
                     /locus_tag="IV223_000027"
     CDS             28701..29012
                     /gene="rhaM"
                     /locus_tag="IV223_000027"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8710342.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            29094..30245
                     /gene="fucO"
                     /locus_tag="IV223_000028"
     CDS             29094..30245
                     /gene="fucO"
                     /locus_tag="IV223_000028"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8710343.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            30372..31130
                     /locus_tag="IV223_000029"
     CDS             30372..31130
                     /locus_tag="IV223_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8710344.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            31230..31874
                     /locus_tag="IV223_000030"
     CDS             31230..31874
                     /locus_tag="IV223_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8710345.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            31881..32888
                     /locus_tag="IV223_000031"
     CDS             31881..32888
                     /locus_tag="IV223_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8710346.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            32904..33746
                     /gene="kduI"
                     /locus_tag="IV223_000032"
     CDS             32904..33746
                     /gene="kduI"
                     /locus_tag="IV223_000032"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8710347.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            33766..34569
                     /locus_tag="IV223_000033"
     CDS             33766..34569
                     /locus_tag="IV223_000033"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8710348.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(34818..35882)
                     /locus_tag="IV223_000034"
     CDS             complement(34818..35882)
                     /locus_tag="IV223_000034"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8710349.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(35879..36964)
                     /locus_tag="IV223_000035"
     CDS             complement(35879..36964)
                     /locus_tag="IV223_000035"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8710350.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(36977..37954)
                     /gene="mvaD"
                     /locus_tag="IV223_000036"
     CDS             complement(36977..37954)
                     /gene="mvaD"
                     /locus_tag="IV223_000036"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8710351.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(37947..38891)
                     /gene="mvk"
                     /locus_tag="IV223_000037"
     CDS             complement(37947..38891)
                     /gene="mvk"
                     /locus_tag="IV223_000037"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8710352.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            39213..39866
                     /locus_tag="IV223_000038"
     CDS             39213..39866
                     /locus_tag="IV223_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8710353.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            39951..40856
                     /locus_tag="IV223_000039"
     CDS             39951..40856
                     /locus_tag="IV223_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8710354.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(40858..41490)
                     /locus_tag="IV223_000040"
     CDS             complement(40858..41490)
                     /locus_tag="IV223_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8710355.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            41810..42334
                     /gene="infC"
                     /locus_tag="IV223_000041"
     CDS             41810..42334
                     /gene="infC"
                     /locus_tag="IV223_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8710356.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            42406..42606
                     /gene="rpmI"
                     /locus_tag="IV223_000042"
     CDS             42406..42606
                     /gene="rpmI"
                     /locus_tag="IV223_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8710357.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            42659..43018
                     /gene="rplT"
                     /locus_tag="IV223_000043"
     CDS             42659..43018
                     /gene="rplT"
                     /locus_tag="IV223_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8710358.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            43270..44607
                     /locus_tag="IV223_000044"
     CDS             43270..44607
                     /locus_tag="IV223_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8710359.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            44655..46784
                     /locus_tag="IV223_000045"
     CDS             44655..46784
                     /locus_tag="IV223_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8710360.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            46759..47448
                     /locus_tag="IV223_000046"
     CDS             46759..47448
                     /locus_tag="IV223_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710361.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(47856..48542)
                     /locus_tag="IV223_000047"
     CDS             complement(47856..48542)
                     /locus_tag="IV223_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8710362.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(48678..48872)
                     /locus_tag="IV223_000048"
     CDS             complement(48678..48872)
                     /locus_tag="IV223_000048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710363.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(48922..49362)
                     /locus_tag="IV223_000049"
     CDS             complement(48922..49362)
                     /locus_tag="IV223_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8710364.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(49366..50211)
                     /locus_tag="IV223_000050"
     CDS             complement(49366..50211)
                     /locus_tag="IV223_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8710365.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            50360..51778
                     /gene="pepV"
                     /locus_tag="IV223_000051"
     CDS             50360..51778
                     /gene="pepV"
                     /locus_tag="IV223_000051"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8710366.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(51835..52782)
                     /locus_tag="IV223_000052"
     CDS             complement(51835..52782)
                     /locus_tag="IV223_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8710367.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(53005..53355)
                     /locus_tag="IV223_000053"
     CDS             complement(53005..53355)
                     /locus_tag="IV223_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8710368.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            53555..54634
                     /gene="pepA"
                     /locus_tag="IV223_000054"
     CDS             53555..54634
                     /gene="pepA"
                     /locus_tag="IV223_000054"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8710369.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            54778..55098
                     /locus_tag="IV223_000055"
     CDS             54778..55098
                     /locus_tag="IV223_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8710370.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            55120..55584
                     /locus_tag="IV223_000056"
     CDS             55120..55584
                     /locus_tag="IV223_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8710371.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            55789..56394
                     /locus_tag="IV223_000057"
     CDS             55789..56394
                     /locus_tag="IV223_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8710372.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(56433..57722)
                     /locus_tag="IV223_000058"
     CDS             complement(56433..57722)
                     /locus_tag="IV223_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8710373.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            58150..>58597
                     /gene="rlmH"
                     /locus_tag="IV223_000059"
     CDS             58150..>58597
                     /gene="rlmH"
                     /locus_tag="IV223_000059"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8710374.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAF"
BASE COUNT        18391 a        10788 c        12554 g        16864 t
ORIGIN      
        1 aaattgtgtg taaaggagtt tagaaattgg attacttaaa aaggttgtta gtaggtaagc
       61 ctttaaaatc tgctgaaaat gatgagcaca aattaacccg atttgctgct ttggcattat
      121 tgtcgtctga tgcgttatca tctattgcat atggtacgga acaaatcgtt gtcgttttag
      181 tcgctttgtc tgctgcagcc atctggtatt cgctacccat tgccgctttt gttattattt
      241 tattgatttc attaacgcta tcatatcgtc aaatcatcca tgcttatcct catggcgggg
      301 gagcatatgt cgtcagcagt gagaatcttg gaaagaatgc tggcttgatt tccggtggct
      361 ctttattgat cgactacatg ttgacagtag cagtatctgt ctcagcaggt gctgaagcga
      421 tcacatcagc ggtgccagca ctctacggtc atcaagtcgc tatttctgtg acaatcgttt
      481 tgctactgat gatgctgaat cttcgtgggc tgagggaatc tgcatcgttc ctattatttc
      541 cggtgtacac atttatcctt gtgatctcgt tactgatcgt tgttggttta tataatatcg
      601 tgacaggtgc tgtgccattg caagctactg cacttccagg agcggctgtg ccgggtgttt
      661 ctatcgcttt gattttacga gcattttctt ctggttcttc ctctttaact ggggtggagg
      721 cgattagtaa tgctgtacca ttctttaaga agccaagagc aaaaaatgcc gctgctacat
      781 tgacgatgat ggcattgatc ttaggtttct tcttcgtggg gatcacattt atcaactatt
      841 ggtatgggat cgttcctgaa aaagaagtca ctgttttgtc tcaaattggt aaagctgtct
      901 ttggacatgg tattttgtat tacgtccttc aatttgctac agcgctgatt ttagcagttg
      961 ctgctaacac cggtttttct gcttttccag ttcttgcata caatctggca aaagataaat
     1021 tcatgcctca catgtatcaa gatcgtgggg atcgactagg ctattccaat gggattatca
     1081 cactagcgct aggttcgatc gtattgctgt ttattttcca tggctcaaca gaacgcttga
     1141 ttccgttata ctctatcggc gtgttcattc catttgcttt atctcaaaca gggatggtcg
     1201 tcaaatggaa aaaagaagga aaaagatggt taagcaaatc gattgccaat attacgggtg
     1261 cgttcatctc atatgcaatc attgcgattt tgtttgtcta tcggttaggg gacatttggc
     1321 ctttcttcat cattatgcca atcgttatgt tcatttttta taagatccat gatcattata
     1381 aaaaagtagc agaacagctt cgtttagaaa atgatgcaaa actacatgat tatgacggga
     1441 ataccgttct tgtattagtc gggaatgtga cacgggtaaa tatcggtgcg ttgaattacg
     1501 cacgttcgat cggtgattat gtcgttgcga tgcatgtttc gctagacgaa gatgtcgaaa
     1561 aagaaaaaga aatcgaagca gaatttaaaa agcatttccc agatgtccgt ttttcaatcg
     1621 tccactcttc ttatcgctcg attgaaaatc caattatccg ttatgtagat atcgtaagta
     1681 aaaatgcagc gaaacaaaac tatacaacta ctgtgttgat tcctcaattt gtaccaaaca
     1741 gacgctggca gaatattctc cataaccaaa caagtttgcg gttaagattg cgattgtctt
     1801 ggcgtgaaaa tatcgttgtc agcacctata gctaccattt gaagaaataa accaagtttt
     1861 aaaatcaaaa acagaccctc tagctcttaa ttgaagctag agggtctgtt ttgtcttatt
     1921 ctcagctaaa ggtgccctct ataagccatt aaagatggga acagcgtatg aaatgttatt
     1981 attcttccta tttagctgta aaacattttg attttgatgt cttgatcata atttccgaat
     2041 cctttgccaa ataatgtaca gcctcctata ttttcagctt cttcctttac ctcgactctt
     2101 aagtgccatg tgtcttcata agtaggaatc tcagcctcgt caagtgacca ttttttgtta
     2161 ccataactca gtgccatttt taattggtga ttcacaaacc caacggtcta agcaagtatc
     2221 aaatcgatag tgaacatgaa tgacaggaga cgaaagaggc aatcattttt gaggcagtag
     2281 aagacttgac ggccaaaatc agttttgaat aaccgttaca aatctttcgt gcgtatcctc
     2341 aggttattca agatatcaat caagtcacac tgcaacgagg aaccttcgtg tattggtgtt
     2401 gttgcataga ctcacaggct ttggattcga cctttgctaa agatgatacg ttgttaccaa
     2461 ttactttacg tttggctacc gctagtcgta aaggagtaag aagtgagcgt ggacaaagat
     2521 cctccctaaa aaggggtctt tttctctatg taaaaaagag gaaaagtttg ctgaatttaa
     2581 gtgctttatt gtattttttt gacgtactta atcaagtacg ctataattat atcagaaaaa
     2641 ggaggagaga caatggctaa tacaacgatg aatcctagca ctgctcgtaa aaatttttat
     2701 cagttattga aagaggtaaa tgaaaatcat actgaaatcg agattattag tgatcgtagt
     2761 ggaaacaatg ctgtattgat tgggctggaa gactggagag cgattcaaga aacccttttt
     2821 cttgagcaaa caggtacttt agataaagta cgagaccgcg aaaaagacga cagtagcttt
     2881 acgaatatcg atgatattga ttgggaagct ctttgatgag taactataca gtcgcaatca
     2941 aaaactctgc gaaagtggat ttaagaaaaa tcaagcaaac gaatttaaag aaacaatttg
     3001 aagaagtgat tcagacattg aaggaagatc catatatgcc cacgcagtct tttgaaaagc
     3061 tgagacctac acatgaaggc agatactcac gcaggctgaa tagacagcat cgagtggttt
     3121 acaaagttga tgaggaaaat aaagttgtag aaatttattc tgcctggaca cattatgagt
     3181 gaagtgagaa gagccaacct ttttagatag ttaacaaaga gtctccccaa aaaggagact
     3241 ctttttttgt tctaaaacca accttcttgg atttattgca tgatttcttt aatttgttcc
     3301 tatatctcca cctttgaagg gtcattgttt gagtttagga atggaatgtt gacgggttta
     3361 tgtaaaacaa ggaggaagaa gtgtgacaag gaaaatcagt aaaattggag catcgttgtt
     3421 agtgttggca gtggcaacga ccatcgatta tcgccaaagt gaggcgaaga caattggaaa
     3481 tgtcgtttat cgagaaacag ccaaaagtat gttgaaaaaa gcaatggaca atcaaccgga
     3541 gtcgtcttat tggttcccag aggattcgtt ggcatggtct tttggaaaag atacagatac
     3601 gaaatacaat accagtgtcg ttcctttagc agagcgggta tccaaagcga cattgccgaa
     3661 catgaatgtc actcaaacgg aagaagttaa agtcgtggcg ctttcggcca tgaatagcag
     3721 tatcaacggc aatgcatcac gagggatcga tatcttttac gccaatgtct tttcttattg
     3781 gcaatatatc gatcaattgg tttacggggg tgctcttctg gtgaaggatc atcgtatcgc
     3841 caagtctagt tgcgacggat gcagtccata agaatcgtgt accggtttta ggaaccgcaa
     3901 acctcccatg gcgagaagct ggaatggcta gatatgtttt tagccaagga tgaggatggc
     3961 aagttcccga ttgtataaaa gatgatcgaa gttaccgata cggcagtcac cagttttgat
     4021 gatgcagctg aaggcatcga acaatcgagg actgatggaa aaggcttaaa tgaaagccac
     4081 gccaaaggat gtaagaattg atgcccaatt caaagaacaa gcagacgagt atttagagat
     4141 catgtggtat gaattccatg acgagtgatg ggaaaatgga ttggcaaaat gccttagcgg
     4201 ataagaaaaa aacttagtcg gtggatgcag atatgaaccc gttggcagta tgaagttgga
     4261 gtatacggaa tggataaata gctaagaaaa gcaactcttt gcccataagg agggaagaat
     4321 cagagtggat tattgcctta aaaatgacag aactaaaaaa gcctttgcga ccaacaaatg
     4381 gtcgcagaag cttttgtttt ttacaataac atcagcgtga tgtacggctg gctggatcta
     4441 gccgtctttc caaaatacct aaaagccagt ccgtgatgat cgccattaat gcagttggca
     4501 atgcacctgc taaaataatc gatgtgccct ctgttgcatt ggttcctcgg ataatgatat
     4561 ctccaagacc accagcaccg acgaatgcac cgatcgcggt tatcccgatg gcaacaacta
     4621 acgcgtttct tatgcctgcc atgataacag aaacagagag tggtaactct accatgaata
     4681 gtcgttgtct agcagtcatc cccatgcctt tgccgacatc aaggatgttt ttgtctactt
     4741 ggatcatccc ggtatacgta ttcttgatga tcggtaagag ggaatacaaa aatacagtaa
     4801 caatcacgac attgactccc aggcctaatc caatgattaa aatggagatc atggctagag
     4861 aagggatcgt ctggatgata ttcgccaaac gaatcaccca gtttgctaat ttacgtctcc
     4921 tagaaatcat gatcccaacc ggaatcccga caactgctgc aaagaggaca ccatagatag
     4981 aaatcaggaa gtgacggata aattgtgcaa agatatagga gccattttcc tggaaataat
     5041 agaaaaattg ttgaaagaca ttcattttat ccaattgctt cacttccctt caaaatagtg
     5101 gttttcttgt aagaaacgtt gtgctactac tgatggttcc actagattat tatctgcttc
     5161 gtagttcaat tcctgcattt tttccgtact gatggtatga cctaacttat ttaaggcttc
     5221 ttttaaatga ggcgtatcag ctaatatctt gttgtctaca actggagcag catcatatgg
     5281 tgggaagaat tgacgatcat cttccaacat gactagatca taactggcga tccgtccatc
     5341 cgtagagtag ccaagaacaa tatccatttt tcctgcttcg actgcgtcat aaacaagtcc
     5401 aatctgcata ggtaatatcg aattgaagga gaagccataa gtttcttgga agccgtcata
     5461 accgtctcct ttacgattga tccaagaagt atctacacca gctactaatt catccgctac
     5521 atttttcaga tcgctgactt tagatagatg gtatttttgt gcggtgtctt ttcgaacaag
     5581 aaagacatac gtattttcaa aactatagga cgggaaccac gtttgaccgt atcttttctc
     5641 aaattcatct ttcacggtag caaacgcttt tttcggatct ttttcaggag gaaggttcaa
     5701 tgtagtcgtt aaatctgttc ctgtgtagcg agccgctgaa attgaagcat ctccgttcat
     5761 catcgcttga tggttgatag ttgtagtagc cagattgttg atgattgcgg tatttttatc
     5821 tgtataatgt tcaaccattc cggcaactag acttgctaag atctgtgctt ctgaagtgat
     5881 tccacctgtg atcgaaatgg tgtcttcatc tgtattgctg gcaagaccag ggaaggaaca
     5941 actcgcaagt ataagaagag agaaaagaac catggtcatt tttagtatac gtttcattct
     6001 tcattctcct ctctaagtgc gatcggtgtc agccggtgtt ccaagagacc tagaagccaa
     6061 tcagccaata gtgctaaaat tgttacaggg atcgtacctg ccaaaatgag atctggttgg
     6121 taattattca gtccactaaa aatcaaatcg cctaaaccac cagcgccgat ataagaggca
     6181 agtgtagccc atgcaatcac gtaaacggca gctaaacgaa taccagccat gatcgttggc
     6241 atagcaatgg gcaattcaac ggaaaaaatg gattggaact cagtcatccc catgcctttt
     6301 gccacatccc gataattcca atcgacattt ttcatcccaa tgtacgtatt acgtaaaatc
     6361 ggcaataatg aataaataaa gagtgcgata atcgcaggta atttacctac gccaaatagc
     6421 gggatcatca atgccaataa agcaagagaa gggattgtct gcaaggcact ggttaagcca
     6481 atcacaatcg tggcagcttt tggaaaacgt gtcagtaaga caccaagcgg tacagcaatg
     6541 atgataccaa tgatcaaagc aagtcctgaa atataaagat gttcaaaacc ttttgagaga
     6601 atttctgaac cgtgctgatg aaaaaactgc tgcataggtc agacctcctt agattcttca
     6661 ggagtagctg actctgcaac atcagtagtt gcgggatctt ctccccaaat cacatcgtag
     6721 acgatatcca ctaatgatgc acgagtcagg atacctacga ctttgttctg ttcatcaacg
     6781 accggcacgt atttcagccc gcgttttaaa atgcgttgca aggtatcacg aagataggcg
     6841 gattctttta cgtaaaaaac atgtttattc aaaatgtcgc cgacactgga tacttttcct
     6901 cgttttttat tcaacgtttc tacatcgata aatcctttta atacattcga attatcggta
     6961 actaacaacg tatctacacg tttttccctc atcagtttga ttgcttcttg taatgatttt
     7021 tccggtgtaa tcgtgattgc tgtatttagc ataacttcgc cgactcttgt cgcatttggc
     7081 ttggcttgta gtaggcgatc ttctccaatc aactcttcta cgaaatcgtt tgcaggatga
     7141 cgtaaaatat tttctggtgt atcaaattgg atcacacggc cttcactcat gatggcgata
     7201 cggcttgcta gtttaatcgc ttcatccatg tcatgggtta caaatacgat cgtttttccc
     7261 aaacgctctt gtaaatcttt gacaaggtct tgcaaggaat cacgagtgat tggatcaagc
     7321 gcaccgaatg gctcgtccat caagatgata tcttgattcg ctgcaagggc acgaacgact
     7381 ccaatacgct gctgctgacc accagataat tcatgtgggt agcgatctaa catctcacgg
     7441 ggcaattcca caagatcaat cattttttca gctgttttat tccgttcttc ttgactgact
     7501 ttcaacaatt tttggacaag agtgatattt tcacggatcg tcatgtgagg catcaatcca
     7561 atactttgga taacatagcc gatctgacgt cgcaattcca ctggatcaat ttcttggatg
     7621 gattttccat caatcaaaat tttcccttgt gttggatcag tcattcggtt gatcatccgc
     7681 atcgtcgtcg tttttccgct tccgctcgta ccgataaaac aaatgaactc acccttttca
     7741 aatgaaagat tgacgtcctc tacagcgact tttccacctt tataaatttt tgacacattt
     7801 tgaaattcaa tcatggtgcc acctcttttt ctattttcaa tgaagaacta tctattacta
     7861 gtatgcctaa aacaatgaaa aaatacaaat agggtagttt ttttatttag gaaaacaaaa
     7921 aatagaagtg aaaaagtgct gattttgcca acagaacgaa aaacatgata gaatgatgaa
     7981 gcacatgata gaaagtgagt gacgatatgt taacaacaga agattttgat tttgacttgc
     8041 cggaagaatt aatcgcacaa acaccattaa aagaccgcga tcattctcgt ttacttgttt
     8101 tggatcggaa aactggagag atggaagata aacatttcca tgaaatcatt gaagagttac
     8161 agcctggtga tgcgctagtt atgaataata cacgagttct gcctgctcgt ttatatggag
     8221 aaaaaccaga tactggtgcg catttagaag tccttcttct aacgaataca caaggagata
     8281 cgtgggagac attgatcaaa cctgcgaaac gtgccaaagt tgggacaaga atctcttttg
     8341 gtgatggcag attgcaagca gtagtgaaag aagaattgga acacggcgga cggatcattg
     8401 actttgagta tgaaggaatt tttctagaga ttcttgaatc attgggagaa atgccattgc
     8461 ctccatatat caaagaacgt ctggaagacc cagatcgcta tcaaactgtc tatgcgaaag
     8521 aaaatggttc tgctgcagcg ccaacagctg gattgcattt tacgaaagaa ttacttgatg
     8581 agatcgaagc aaagggtgtt gaattagtct atctgacact acatgtaggg ttaggtacat
     8641 tccgacctgt cagcgtagat aaaattgaag aacaccatat gcatagtgaa ttctaccgtt
     8701 tgacggaaga agcggctgag cgattgaacc aagtacgtaa aaatggcggg aaaatcgtgg
     8761 ctgtcggaac aacttcaatt cgaacattag aaacgattgg aacgaaattt aatggagaaa
     8821 tcaaagcaga cagcggatgg actgatattt tcattacgcc tggctatgaa ttcaaagtgg
     8881 tagaagcttt ctcaactaat ttccatttgc caaaatcgac tttagtcatg ttagttagtg
     8941 cctttgcagg aagagaattg actttatctg cttaccagca tgcgattgaa gagcgctatc
     9001 gtttctttag ttttggagat gcgatgttcg tgaaataagg ttatgaaaag ccgctagttt
     9061 tgaataacaa aaaagcttta caaagatttt atgacaaaga aaaagccgag ctctcgataa
     9121 gctcggcttt cttgtacctc ggaaggattc aaataaacag aaatgattgt aactttccat
     9181 catacatgca aagcacggtg ttgaatttct tcatgttaaa caaaaatttt gttttcgtta
     9241 catcttcaac atttgaaaca tctcttgttc ctattcaatt ttcagacgtt tacgttattc
     9301 aattgttacg tatcgcatta cttcttgttt cggatacttc gtgatccaaa catccatacg
     9361 cattcactat ttataggttt gtactctaca cgcatgtctg ggtgaaacct gtacttctaa
     9421 caactttgtt tgcttacact gttctatcga gaaattcttc tgtctatttg atggtatcct
     9481 ccttccttac atctatataa tagcatcctt tggtagcgtt tgcaaatata agcactttaa
     9541 attttacgct ttcttaacaa ataatcgata agccctttat ttgaagtaaa taaaaaaagg
     9601 atgttatagt aaaataagta cctttttttg tacgaagatt ttaaaggagg aataggatgg
     9661 aagaatacaa gcagacaata gaagaagtaa agaaggtaac agaagcagat ccagatactg
     9721 ggttgtcttc acaagaagtg gatgaacggc gaaaaaagca gggattgaat aaattcgatg
     9781 aggcgccgaa agaatcgatg atcaaaaaat tctttcgaag cttgtccgat tttacaacga
     9841 tcatccttct tgtagcagcg gtgatttcct tttataccgc atttgctaca gaacatgggg
     9901 atttatttga aggactgctg atcatcgcta tcgttgtgat caactcggta ttggcgattg
     9961 tccaagaagg aaacgcagaa aaagcactgg aatccttgca agatatgaat aaacaaacgg
    10021 caactgtcat acgagacggc aaagtgacca ctgttgagtc agagcaactg gtagtgggtg
    10081 atattcttgt tttagagtcc ggtgatgcta tttctgccga tgcccgtttg atcgaagcat
    10141 cacaattacg tgtagaagaa tcggcgttga ctggagaaag tgaagcagtt gaaaaagatg
    10201 cagcatttat tgctgaagaa gatgagtcat taggtgacca acttaatatg gtatttaaag
    10261 gatgtacggt tgctgcaggt cgtgggaaag cgatcgtcac tgccattggc atggctacag
    10321 aaatgggtaa gatcgcggat cttttgaacg aaaatactat gcaaaaaaca cctttacagg
    10381 tccgattgaa tcagctaggc aaacgaatca gtatgatcgc tttagcggct gcggctttag
    10441 tatttgtgat tggtgaactg caaggagaac cactccttga gatgttcatg acttcgattt
    10501 ccttggctgt tgcagctgta ccggaaacct tgactgttat tgttacgttg acacttgcat
    10561 acggtgtgca gaagatggcg aggaaacatg cgatcatccg acagttgcca gcagttgaaa
    10621 cgctaggaac cgctaatgtt atctgctcag acaaaacagg aacgctgaca caaaatgaaa
    10681 tgcgcgtccg ccgggtatgg agcaaagaag atgaagtaac gaatatcgaa gattcaatga
    10741 cgaacagcgc gatggaaata ttgaaaatgg ctgcactatg tacagatgtg acggtcgagc
    10801 aagaagatga cgaccttgtt atcaaaggta atccaacaga agtagccatc gtaagggctg
    10861 ttgaagagaa ttatcataca aaggcagaat tagaagaaaa atatccacga gtaaacgagc
    10921 ttccttttga ttctgaacga aaaatgatga caacagttca ccaaatgggc gaaaaataca
    10981 tctcaatcac caaaggagca ttcgatgttc ttgctccgcg ctttagttca ggcgatgtag
    11041 aacaagcggc gatcgttaat gatcgctttg gtaaacgggc actgagagtg attgctgttg
    11101 gttatgctac ttatgacgaa gaaccgcaag atatttcttc tgaagcactg gaaaaagact
    11161 tacgtttgat tggtctgatc ggaatgatcg atccaccacg tccagaaagt aaaggagcaa
    11221 tcaagcgagc gaaaaaggct ggcatcaaaa cagtcatgat cactggagat catgtggtga
    11281 ctgccagtgc catagcgaaa gaattaggta tcttgaatga tccgtcagaa gcactttcag
    11341 gatcagaact gcatcaatta tcagacgaag aattagatgc gcgtgtcaaa gctctctctg
    11401 tctatgctcg ggtaacgcca gaagacaaga tccggatcgt caaatcttgg caacgtacgg
    11461 gtgcagtcgt agcgatgact ggggatggtg tgaatgatgc accagcgttg aaagcaagta
    11521 acgttgggtg tgccatgggg atcacaggaa cagatgtggc gcaaagtgcc gcagatatga
    11581 ttttgacgga tgacaacttt gcgacgatcg tagacgctgt cagtcaagga cgttcggttt
    11641 atcagaatat tcgtaaagct atcaactttt tgctaagctg taatatttcg gagatcttta
    11701 ttgttttgat tgcgatgctg ttaggatggg gagcaccatt tacagctgtg caactactgt
    11761 ttgtcaatgt agttgctgat ggacttccag gatttgcttt aggaagagag ccagcggaac
    11821 acggcatcat ggatcagcca cccattccga aaaatgaagg gatctttgct cgaggtttat
    11881 tgcaaaaaat tgccataaat gcaggggtct tcaccatcgt aaccttgttt ggttattatt
    11941 taggtagtta tgtcgatact atcagcccat gggtagacgc aagtcagcat gtcggtcaaa
    12001 ctgttgcatt cttgatctta gcttattcat cgattctgca tgtgttcaat gtacgcagca
    12061 gccaatcgat cttcaaagtc aatttagcga ccaataaagc tttggtagaa atggcgttac
    12121 tggcattagc tattacaacg gctgttgctt tgctgccatt tacgcaagaa ctattcggcc
    12181 tagtacacat cagtttgaat cactggttct tggtagggat tttatctatc gtaccaatcg
    12241 cggtgaatga actgatcaag ttccatcgat taccggaagc agaagaagag gaatgattaa
    12301 ctgtttctaa gttgattttc tttttactgg aactatacaa aaaggaggtg tgacatgaat
    12361 gttgtcacac ctcctttttg ctaatgggct gaatgtttct tttactgcct ctcgataaac
    12421 ggtgttctcc tgccaccccc tcggtcacaa gtcacactct cttttaaata tcttttggca
    12481 attgttcata aaatgctgct tttgttgcag aaatgtatgg gctgagccat agataaccaa
    12541 taccagcagt tgctaatgct aagatatgcc agccgataaa tgaaagatct aaccagaaaa
    12601 gttgtccttt ataaccatcc atcaattttc gacttgcagt gatcgtatcc agtactttag
    12661 gtgtttgtcc tgtttcttgg acgatatcgt agtaaataaa atagctttga gaataagaat
    12721 agcttttaat gattccagga ataactaata gcaaacccca taatgttgta aagatcgaga
    12781 tcagcacagc taagctgatc actccgccga taaacaaacc tttgaatcca cgaaatgcat
    12841 ctttgaatgg ttcgatctga tctttttgtc cacgtaaaat atcaagataa gtccaagaaa
    12901 taccgctcat gaacaaagcg ctaatgatgc tggaaataat tccgccaccg cctcctgaac
    12961 taccgcctcc tgaagttgtc gcttctgaga cgctcgctga tccatcatat atggtggtgg
    13021 caaacaagat aataggaatc ataataagaa gaatagtagc aataccaatc aatgtaggga
    13081 ttagatttag taaaacagct tgtccccaac gtccttttag actctctttt gcttggcgtt
    13141 ttaattctgc agaagattgc atgatttttc cctccttttc tttaataaca ccaatatacc
    13201 atgtatcaaa aaatatttct gtcataatgt ttttcttttt tttgttattc ataaaaaaag
    13261 gataaaaaaa agaatattaa ataaagtaga aggaagccga gggaacttgc aaaaagcact
    13321 tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta gggaaatgaa
    13381 tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact ggcgcgcgtt
    13441 taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg ccagtaggaa
    13501 cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt gctggcgtta
    13561 ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta gaagaagctg
    13621 gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca ggcggctttc
    13681 aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat ttcaaaaatc
    13741 acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc caaaataaat
    13801 tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa agttacgact
    13861 acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg aaagctcatg
    13921 ctaatccaga ccgtcaagga ttatttggaa tcatccaagg agcagggttt gaagatcttc
    13981 gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca atcggcgggt
    14041 tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact acaccgttga
    14101 tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca ttgatcgatg
    14161 gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc gctcgaaatg
    14221 gcacatgtat gacttctcaa ggccgtctag tagtgaaaaa tgcgaaatat gcccatgatt
    14281 tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca cgtgcctata
    14341 tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct taccacaatt
    14401 tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat gataatttgc
    14461 ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac gcaaaaagct
    14521 tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttatcagca tttttctgct
    14581 aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca ttaccaatgc
    14641 ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg caaaagaaac
    14701 aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca gtcgtaacga
    14761 tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca gttgttttag
    14821 actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc aaaccaggga
    14881 ctgtcgtaac aaatgatgca acgactactg ttgaaacaac agaagaacca gctgagacag
    14941 ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag gaagaaaaat
    15001 aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc tcatttttta
    15061 ttcaaaaaag aaagagataa gagcatggga tatattgctc aatattgaat aatcgggtgt
    15121 gtcatataaa taataataaa taactttata tatttttcta tacaaacacc tgtaaataaa
    15181 gcaattttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa
    15241 ataaatattg tatgatatac atgtgaatta tttaacaaat aaccatgacc cattaggagg
    15301 ataatgatgg ctaaagtaat agaaaaagaa aaaacgaaga caattgacgt acaagcaatg
    15361 attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag ctttgatcaa
    15421 gaaaaagtag accatatcgt gcatgaaatg gcaatggcag ctttggatca acatatgcca
    15481 ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa
    15541 aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac agttggtgtg
    15601 atcaatgaag atgtccaaaa agggttgatt gaaattgctg aaccagtcgg tgtggtttgc
    15661 ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat gatcgcgttg
    15721 aaaacaagaa atccaatcgt ttttgctttc catccaagtg cccaaaaatc ttctgcagaa
    15781 gcagcacgag tggtacgtga tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa
    15841 tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg
    15901 atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac aggaaaacca
    15961 gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc aaaaattaaa
    16021 cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat ttgtgcttct
    16081 gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga attccaagca
    16141 caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc agtgatgaac
    16201 gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa
    16261 ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta
    16321 ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat gatcaaagct
    16381 aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg aggattagga
    16441 catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg
    16501 aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg tgatatctat
    16561 aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa ctctgtttct
    16621 cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg gagaaataac
    16681 atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct attgtactta
    16741 gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat ggtccaattc
    16801 ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa
    16861 gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg aacgaaactg
    16921 atggcagact tcaagccaga tacagtaatc gctcttggag gcggttctgc aatggacgca
    16981 gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg tgcaaaacaa
    17041 aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa aacacaattc
    17101 gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc ggtcatcact
    17161 gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc agatgtcgca
    17221 atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga taccgggatg
    17281 gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga ttatacacgt
    17341 ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc agtaaaaaca
    17401 cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg tatggcgttt
    17461 gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg ggaatacggg
    17521 atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta taatgcaaaa
    17581 gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga tacagattac
    17641 gcagatatcg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct
    17701 ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag tctaaaagct
    17761 caaggagtca cacaagaaac actagataca accgtagacc gtatggctga attagcttac
    17821 gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt gaaacagatc
    17881 atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata agaaaataga
    17941 tgaaaaagaa cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg
    18001 aaagttactt ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg
    18061 atatttatag tggacggtat tactgctata ttaaggatat aaaatgaaag cgtttaaaaa
    18121 ggtggcgtaa agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa
    18181 aaaattgacc tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg
    18241 ttggactttg atggactgaa agtagatgac aaaagctgga cagtgtggaa ttggccacaa
    18301 ggtgtcggtc tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat
    18361 caagtggtca atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc
    18421 aatacaatgg caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc
    18481 tatttgcctt atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat
    18541 gaagacgggt tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat
    18601 acattgatga tgacagtgct tcctttggca aaaatcggga aactgttgaa tcgcccagaa
    18661 tatttagaag aagctcgtta ccagtttatg atccatatca aatacttgat ggataaacga
    18721 acaggattgt ggtatcacgg ctggacgttt gaaggaaatc ataactatgc cgaagcattt
    18781 tgggctcgtg gaaactgctg gttgacgatt gctattccag agatcattga aatcttagaa
    18841 ttatcgaaag aagatgcatt gcgcaaatta ttgatcgaaa cacttgaagc acaagtgcgt
    18901 gcattaaaaa cttatcagtc tgaaagcggt ttatggcata cgttgttgga tgatccttct
    18961 tcttacgtag aatcttctgc aactgcagga tttgcttacg gtattttgaa agcggtccat
    19021 aaacgttatt taccacaaga atataaagaa gtcgcttatc gtgctatcca aggattgctt
    19081 gaacaaatcg atgaaaaagg agaagttcaa aacgtttcaa tcgggacagg aatgggggac
    19141 agtttagatt tttatcggaa tatcggaatc acagcaatgc cttatggaca gtcgttaacg
    19201 gtgctttgtt tgacagaatt gctcgtctct tattgttaaa agaaaggaaa cccatatgaa
    19261 aaaaacatca ctgctgtttg cagcaagttc gcttagccta ggtctgctag gtctaagcgg
    19321 ctgcggaaat aattctagtg tggcaactgt cgcagatgca gatgtaaatg agatagttac
    19381 cttgcgcttt gcttatgcaa gcaacagcca gccagtaatt gatgcaatga atgaatttgg
    19441 gcgtttagta aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca
    19501 actgggtgga gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa
    19561 agtaagtgcc tcagcgttgg aaagtttctc taaagattat tcgatttttt cagttcctta
    19621 tctattcaat gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc
    19681 aatctaccaa tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca
    19741 gcgcagcttt tatatggtag acggtccgat ccatacgccg gaagatttga agggaaagaa
    19801 gatccgtgtg atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc
    19861 cgtacctatg ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc
    19921 agaaaacaat gaatttgtct tgtatacagc tggacatggt ggtgtggcta aatactattc
    19981 ctatgatgag catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca
    20041 gctaactgat gagcaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga
    20101 aattgaagct tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg
    20161 agtccaattc aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga
    20221 tatattcaaa aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc
    20281 gtagctgtga tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt
    20341 gttgggatgg tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca
    20401 aatacggtca cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc
    20461 gcgtatgttg tgggacaaaa agcacattta gccgtaacgg ttttaagtga aaaattaact
    20521 ggcaaaaaaa gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg
    20581 atgatcatgg tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct
    20641 tctttaagta ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc
    20701 attatctata gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa
    20761 aaaatataaa aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta
    20821 tttgttgtgt tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg
    20881 atgactttat tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg
    20941 gtgggaagta tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt
    21001 atcatgaata atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg
    21061 cgcgtgccgg gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc
    21121 tctagctcgg ccattgccgc ttctaccgcg attggcggag tgttgatccc tcagcaagtg
    21181 gaagaaggat atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt
    21241 atggtcattc ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg
    21301 atctctgctt tgtttatggg aggttatctc gtaggcgctt tgtgggcgct aggaatcatg
    21361 ctagtagcat tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa aggacagatg
    21421 gctcatgctg gaaaaataat aaaagaggcg attcctagcc ttctattgat tgtcatcatt
    21481 atcggtggta tcttgacggg gatatttaca gcagtagaag cttcagcgat agctgtcatt
    21541 tactccctgt tgattgctat gttttactat aaaacggtaa aactaaaaga tatacctaat
    21601 atgttgtttc aatcagtagc aatgtcaggg acgatcatgt tcttattggc tacttcttcc
    21661 atgatgtctt tcgccatggc gtttaccggt attcctcagg caattagttc gttgattatg
    21721 ggcgtaacgg ataataaatt tattattctg ttattagtca atcttgtgtt attactggtg
    21781 ggtatgttta tggatgtggc gcctgctatc ttgattttta caccaatatt tttaccaatc
    21841 gtcacaagtg taggggttga tcctgtacat tacgggttgt tttcaatcat gaatctgtgt
    21901 gtaggctcca tcacgccgcc agtaggtaca ggactatatg tcggtgcaag tgtaggaggc
    21961 gtaaaagcag aggcaatgct gaaaccgctt cttccttttt atggggttat cttagtcgtc
    22021 ttgtttttga ttacttactt cccgcaaatc gtcatgtggc tacccaatat gctgagttag
    22081 gagaatagtt atgttgaaca ttacaattag aggacatgat ctttccgaag tccaaacgat
    22141 agaagatctt gcagaaaaaa caaaagaaca agggattcat acactgcagc tcgccttagg
    22201 tctttcgttt cctgaaatac cttctggcgc aaatgagatc aattctggga tgggaaatta
    22261 tgtcaaacga gttttagaaa agcaagaagt gaccgtagga attttaagtt gctatatcaa
    22321 tatgattcat ccagatttaa cgatacgaca agagttgctg acaaagttcg agcagtatct
    22381 tcgctatgct tcttcctttg gtgcgtcgat ggttgcttcg gaaactggat gtgttttgcc
    22441 ggaaatccaa tatacggaag aaaatttcac agatgaagca tttgctgaag ctgtgtccgt
    22501 tatccgtcgc ttggtaaaag ctggagaaaa ataccaaatg atggtgggga tagaaccagg
    22561 tttgaatcat ccggtgtatt ctttagcaag ggtagagcag ctgatccaag cagtagattc
    22621 ggactatcta ggaatcattt tagatccgac caatttgatc acaagtagtg attatcaaga
    22681 acaagtccaa ttggtagaag aagcttttga acgttttggt gaaaagatat gtgcggttca
    22741 tttgaaagat tttcgtgtcg aacaggaaaa aatcgtgccg gtaaatttgg gagacggtgt
    22801 gatcgagtac acaaaaatca aagaaatcat catgaagaat cgtccgtacc tgtatgtggt
    22861 tcttgaagaa acaaaagatg atgggattcg ttatggaaga agtcttcttg aataatgtaa
    22921 agaagacgtt ggttaactaa tatttagaat ggagggggcg gggctagaag atgccacgtc
    22981 tcttcttttt tataagagta tataaaagca gctctttttg attacaaaaa acgtggtata
    23041 ataaaattgt gcataagtga acaaaaggag gtgcggcaca tgctccaaaa gaaaatcagc
    23101 ggtgaacaag tcaatacgat ggtttctgat cagtatgaaa tctatcatgt caaagatgtc
    23161 gtcagccaaa ataaaacgat ctatcatcat catgatttct atgagatcca tgctacatta
    23221 aagggagaag cgacttttta tttgaacgga cgacaattta cgatcaaagc aggaagtgtg
    23281 ctgctcatcc attccaatga tttgcatcgt atcgtccgcc aatcttcaga tgtattcgag
    23341 cgtgtctata tctttgtcac cccttctttt ctagaaagtc gttccacgcc atggtctaat
    23401 ctatcggctt gttttcaacc aatgggagag aagaggagtc gtgtattgca aatagaacag
    23461 gaagtattga agcaaaaatt atcatttatc gaccatcctc ctgataaaaa aatttacggt
    23521 gaagacataa aatatgaaca agctctggta gaatacttga tttttctcaa tcaagttgta
    23581 caaaaagaag agagtgcgca agaacagaat gctgtagttc ctaatgagcg tgtggaacaa
    23641 atgttacaat atatctcaaa aaatttatct catccgcttt ctttagagga aatggaaaag
    23701 cagttttttg tcagcaaata ccatatcaca cgagaattta aaaagtacac tggatttacg
    23761 tttcatcagt atgtaatgaa gaaaaagctg atctatgcca agcacttgtt gcgagaatac
    23821 cgcagctcta gtgctgtata tagtaaaagt ggtttttctt cttatccgca ttttctgaaa
    23881 gcattcaaga gagagttcgg cataacgcct aaagaatttt tgaaacgtga tacacagaaa
    23941 cagttggttc attatgaaca ttttgaagat gaacaatgag aatgatcact aaaaaataac
    24001 cgcagtagct gctgttgttt acatagaagc agaaactgcg gttattttat ttttatctat
    24061 atatttcaaa atattttgaa aaaatgacgt acatattata tgaatcagct gaatttacga
    24121 aagtctctcg gcgatagtcc tgtttttgct ttgaacaggc ggctaaagta agcaaaattc
    24181 tcaatcccta tttcttcgga aatcattcga accgtaaatt ctgtataccg aagaaggtct
    24241 ttagcgtaag aaagtttttt gctgatcaaa taatcgattg gtggagaacc gataaatttg
    24301 gaaaaatctt tgctcagctg atatttattc aataagaatt gttgttctaa ttgatcaagt
    24361 gtaatcgttt ctttgaaatg ttcgtctaag tattgcttca gtttgtaaac gtaggcggga
    24421 atatcctctt cttcgaaatc tagctgatac ttttgcaaaa tcagttcatt cagcaactcg
    24481 tggatagcta cagaggactg gaaatcggta cgggcattcg tgccttgttg catgtggatc
    24541 aattctgtga tcaaatggtg gatcttgttt tctgatggat ctcctgttga atggaaggtg
    24601 ttatggccgt cttttgtgaa ctcctgataa aatgattgga cgttctgacc attcaaatgg
    24661 atccaatcca tttcccatgg ttcttcgctg atcgtttgat aacgctgata ttcttggcag
    24721 tcaataaaaa ataagtctcc agcttttagc tgttgctgat tatcattata tgtaagcgaa
    24781 cctttgccag acaaagtaaa ctttatcaag taagaaggta aatgtgctcg ttcagtatag
    24841 taagggattg gtgctttgaa gtaaccgatt tcttggatat aaaaaaataa attcctgctt
    24901 ctttgtgtag gcgtcaagat cactcttttt gagtcttccg accatgagtg gttggctttt
    24961 tcccagtaat ccatgtaaac actcccaact tgtcaagata atgcaattat ataccaaaat
    25021 agtgtgaagc aaaatataag attctgtatt atgatagcat tgtcaataaa tgataacgct
    25081 ttattaataa agtgctaatc aacaataaag taaaagaggt ggagaaatga atccaacatt
    25141 tgttgccatc gatatcggtg catcaagcgg acgactaatg aaaagtacga gggatgcaaa
    25201 aggttctttg atcttggaag aagtacaccg gttcaaaaac ggctttcgga acaaagacgg
    25261 tcacgatcgc tggaagatcg attatcttat caaagagatt ttgattggat ttgaaaaatt
    25321 aaagcagaca gggattgatc gcctttttgc ggggatcgat acatgggcag tcgattactg
    25381 tctgattgat gaacaagggg atcggataaa tgatccgatt gcttatcggg acaagcgtac
    25441 caatggagca gtagaacagt tttctgaaat ctattctcta gataagttgt atgagcagac
    25501 agggattcag atccagcctt tcaatacctt gttccaattg tttgctgaag aaaaagaaaa
    25561 tttggcaaaa gcacataaat tgttactgat acctgattat ttagggtatg tctttactgg
    25621 taacatggtg acggagaaaa ctaatgcatc tactatgcag ctgctgaatg cagggacaaa
    25681 agaatgggaa atagaacttc ttgaaaaaat cggtgtctcg aaggaattat ttccaccatt
    25741 gacagagccg ggaacgatac taggagatct gcaaaaagaa caattttcaa aatatgattt
    25801 gccagatgtg acctttatca cagtagcaag tcatgatacg gcgtcagctg tattaggaac
    25861 gccaggtcaa ggagaacgtt ggggttatat cagcagtggg acatggtcgc ttttagggat
    25921 tgaaacaaag atcgccaatg tttctcgcaa tgctttttct gaaaattata cgaatgaatg
    25981 gggtgcccat aacacggtgc gttttttgaa aaacatcatg gggatgtggt tgatccagga
    26041 agttgcccga atgcaagagt acgagtacag ttatgcggaa ttggcagagc ttgctgcaaa
    26101 agaaaaaccg ctgcagcaag tgatggaaat caatgaccct cgatttttga atcccccaaa
    26161 tatgatagaa gagattcagc aatactgttt tgaaacagag cagccagtcc ctgtctcatc
    26221 gggagctatc gctcgttgtg tgtatgataa tcttgctctt tgttacgcac aagagctgat
    26281 caaagtagaa cacttaactg gaacgaaatt ggaaaagctg cacatcgtag ggggcggatc
    26341 gaataatcgt ctactgaatc aattgacagc agattatgcc ggaatcattg tagaagcagg
    26401 acctagtgaa gccacagcaa tcgggaatgt tgccatgcaa atgctcagta caggtcagtt
    26461 ttcgaatata gaacaagcaa gaaatgtaat caaacagtcg tttccatgtg aaacttttta
    26521 cccagatgaa aataagcaaa acacgatgga atggtacgtg aataaaatca aaggagtaat
    26581 cgtatgaaca cgattgaaag aaattacgaa caagcaaaag aaaaatatgc aactatcggt
    26641 gtagatacag atgctgtact agaaaagata caggacatca aaatttctat gcattgctgg
    26701 caaggagatg atgtcaaagg tttcttgaca ccggatggcg aattgaccgg tggaatcatg
    26761 gcgacaggga attttccagg agccgcacga acacctgaag agcttagaca agatctggag
    26821 aaggcttatt cgttgatacc gggcaaacac aaattgaacc ttcatgctat ctatttggat
    26881 acagaagaat ctgtcgatct aaatgaaatt gagccgaaac attttgaaaa atgggtagag
    26941 tgggcaaaaa aagaagaaat cggtcttgac ttcaatccga ctttcttttc gcatccaatg
    27001 atgaaagacg gctttacatt ggctcatcca gataaagaag ttcgtgaata ctggatcgaa
    27061 cacgggaaaa gaagccgcaa aatcgcggag tactttggga aagaaactgg gcaaacaagt
    27121 atcaataact tttgggtacc tgatgggatg aaagataatc cgattgatcg ttttacccca
    27181 cgggaacgtt taatggaatc actcgatgaa atttttacag aagagttaga tgaacggtat
    27241 gtacaagatg cagtagaaag caaactattt ggtttaggcg ctgaagctta tacagtcggt
    27301 tcccatgaat tttatatggg ttacgggttg actcgtgaca aacttgtttg tctagatgct
    27361 ggacattttc atccaacaga agtgatttcc aataaattat cttcattagc tttattcagc
    27421 aaaggaatca tgttgcatgt ttcccgtcca gtgcgttggg attcagacca tgtggttttg
    27481 atggatgatg aattgcagga aatcgctaaa gaattagtcc gtaacgaatt gttggaaaaa
    27541 acaaatatcg gtctggattt ctttgatgca acgatcaacc gtatcgctgc ttgggtcatc
    27601 ggtacgagaa atacacaaaa agcactgctg aaagctatgc tggaaccagt tgagcgattg
    27661 gaagaaatgg agctggcttt tgactttact tcaagaatgg cttatacgga agaattgaaa
    27721 gatttcccgt atgccgatgt atggaattat ttctgttaca aaaatcaagt tccagttggt
    27781 ttggattggc tggaagaagt ccaggaatat caaaaagacg tgttggaatt aagaaaatag
    27841 gagtgtggct atattgaaaa aaatagatat tttacaagcg ccttatgtga aagaaatgat
    27901 cgaaacgacg tcgaatcttt atcgcttggg atgggatgaa agaaatggcg ggaatatttc
    27961 ttatttacta aaagaagaag agatcacacc gttcttggat cctgaaaaag tgatccgccg
    28021 catctcgatg atttttgatg ccagccgttt agcaggatgc tattttattg taactggttc
    28081 aggaagatat tttaaaaatg tagcgagcga acctgccgag aatcttggaa tcgttcgagt
    28141 tgctgaagat ggcaaaacac tggaactttt gtgggggctg gaaaacgaat gccttcctac
    28201 aagcgagttg ccaagtcatt tcatgagtca tatcgcccgt ttagaagtag acccagataa
    28261 tcggatcgtt atgcattgcc atgctagtca tctattagcg atgagtttta cgcatgaatt
    28321 agacgaacgt tcctttagtc gtacgctatg gcaaatgtgt acggaatgct tagttgtttt
    28381 tccagaagga atttccatca ttccgtggat ggttccggga acaaatgaga ttggggaagc
    28441 aacagctgaa aaaatgaaag aaactcgatt ggttttatgg ccacaacatg gaatctacgg
    28501 gtcaggaaaa gacatggatg aagtctttgg tttgatcgag acagcagaaa aggcagcaga
    28561 agtctatacg tacgtcaaag cgcaaggacc aatcttgcaa acaatcacgg acgaaaacct
    28621 gtggcattta gcagatgcgt ttggtgtcac tccaaaagca ggttatttgg aggaagttca
    28681 tacgaaagca ggtgtctgac atggtgaaaa aagctttttg catgaaagtt tacccagaaa
    28741 aacaagaaga atacaaaaaa agacatgatg aactttggcc agaaatgcgt acagcacttc
    28801 gtgaacacgg ggtcatcgac tattcgatct atcttgatcc gagtacaagt ttgttatttg
    28861 gttacttagt gatcgaagat gaaaagttat gggaaaaaat ggcaaataca tctatcaatc
    28921 aaaaatggtg gaagtacatg gaagatatca tggagacaaa tccagatgcc tctccagtca
    28981 gttgggaatt gatgccagtc tttgaactgt agcaacaagc aactgggcaa ccggttgctt
    29041 ggggttcttt cgtttttatt gtgaaaaata atacaaatta ggaggaatag aaaatgagta
    29101 atcgaatgat tttaaatgaa acatcgtatt ttggaaaagg agcgattgag tcgatcgtcc
    29161 ctgaatttca gcagcgagga ttcggcaaag cagctgtcat tactgacaaa ggattgatcg
    29221 agcacggtat agcaacaaaa gtaacagaac ttctagataa agcagcaatc gcttatgctt
    29281 tatacgacgg gatcgtacct aatccaacga tccaaaatgt aaaagatgga gtggcttttg
    29341 ttaaagaggc agaggcagac tgtttgatcg caatcggtgg aggttcgcca atcgatacgg
    29401 caaaagccat tgggattatt ttgacgaatc cagagttttc agacgtcatt agtttagaag
    29461 gtgttgctga tacgaaaaat ccatgtttac ctatattagc tataccaacg acttctggaa
    29521 cagcagcaga agtaacgatc aactacgtca tcacagatga agtaaatcac cgaaaatttg
    29581 tttgtgttga tcctcatgat atcccaatcg tcgcgttcat cgacagcgat atgatgatgg
    29641 gaatgccgaa aaaattggct gcatcaacag gtatggatgc tatgacgcat gctattgaag
    29701 ggtttatcac aaaaggcgct tgggaaatga ctgatatgct gcatttaaaa gcaatcgaaa
    29761 tcatcggcca ttctttggaa gcttctgtag atggcgatca aaatgggcga gaaaaaatgg
    29821 cattaggaca gtatattgcg ggaatgggct tctcaaatgt cggtttagga ctggtacacg
    29881 ggatggctca cccgttaagt gcatggtaca acattcctca tggcgtagcg tgtgcagcac
    29941 ttttgccaac tgtcatgaaa tataacaaag aatatactgg agaaaagtac cgtgagatcg
    30001 ctcttgttct agggattaaa ggagcagctg aaatgtcttt agaagatgta agagaagcgg
    30061 cttgtggtga gatcgatcgg ttgagcaaag cggttggaat cccagaaacc atttctgaat
    30121 taggtgtaaa agaagcagat attccagcaa ttgcagaaga tgctttgcga gatgtatgca
    30181 caccaggaaa tccaagagaa acgacagtag aagaaatcat tgctttatat caaagtttga
    30241 tgtaaaaacg aagcggatct tcaacattaa attgcctgta aagagaaatg gtagtttata
    30301 tctggatatc tcttttaaat tgatggtatt tccgctataa tttttcttat aaagaaatag
    30361 gagaatatta tatggaaaat aaaaagcctt atggaactgt attgatcaaa gcctctaaaa
    30421 tactagacta tttagcggaa aaccaagatg tctctttgca gacgatcgca aagggtgtgg
    30481 gaatgacctc ttctacagtc ttgaaaatac tagatacttt gttgatgatc ggctacgtaa
    30541 ataaaaattc agagaaaaac tatcgtttag gtgcaaagct tattcgctac gcgaataaaa
    30601 acattgaaca aatcgatttg gtagagttga ctttaccgtt tttagaacag ctgcagcaaa
    30661 agatcgatga gactatccac ttaggggtat tggacaacaa tgagatcttg tacgtgaaca
    30721 aactagatcc aaaacatcaa acgatccgca tgtcttcaaa aatcggaatc acccgaccat
    30781 tatacaattc tgcgatgggc aaagccgtat tagcagaatt cagtgaagag cagtacactg
    30841 attatcttga aaagcatccg ttgatccctt acacagaata tacgatcacc aatgcattac
    30901 ggttgaaaaa ggaaatcaaa acggtaaaag aaacccacat cgcatttgac gatgaagaaa
    30961 tcgaacgtga tattttttgt ataggagcct caatcgtgaa aaacgaacaa atcattggtg
    31021 catttagtgt aagtatgccg aaatatcgtt taacagatga gacaaaagaa caaataatca
    31081 gtgccctcaa gcaaacaaaa agcgaaatcg agcaaagaat tcaaaaataa tgaaaaaagc
    31141 gtttgcaatt tttcttttct gccgtataat atgggtatag atttccaagt tataaaataa
    31201 aatttctaaa taggaaatag gtggagaaga tgaaaaaaat tgatatttta tctcgattga
    31261 aaaacgccgg agtgattgcg gtggtacgcg gaaaaagcaa agaagaagct ttgaatgctt
    31321 gccacgcaat catcaaaggt ggtctaactg gtattgagtt gacttttaca gtccctcaag
    31381 cagatcaagt aatcaaagaa cttttgtcat tctacaaaga ccaaccagaa atagtgattg
    31441 gcgcaggcac agtattagac gctgtcacag ctcgcttggc tatcttagct ggcgcagagt
    31501 atattgttag cccttccttt gatcaagaaa cggcagagat gtgcaatctg tatcaaattc
    31561 cttacttacc aggatgtatg actattaccg agatcaaaac agcgctgaaa agcggggtag
    31621 atatcgtcaa actatttcct ggaagcgctt atggaccaag tatcatttct gccttcaagg
    31681 cgccgatgcc tcaagtcaat atcatgccaa ctggcggtgt gagtctggac aatatgaaag
    31741 agtggttcga tgctggtgtc gtaacagtcg gagtaggtgg gaacctttta gcacccgcag
    31801 ctacaggaga ttttgacaaa gtaacggaag tcgcgcaaca gtatgcagca aaaatgaaag
    31861 aaatcaagag gtagctgctt atgggaaaag tagtgacact cggagaaatc atgttgcgat
    31921 tgtccacaga gcagggaacc cgtatcgatt caagtgaacg attcgctgcc cattatggcg
    31981 gaggggaagc aaacgtagca atttctttag ctaattacgg acatgaagcg atttttgcta
    32041 caaaagtacc tgataacgca ttgggaaaag cagtcaaaaa gcacttgcaa tgttatggag
    32101 tagatacgaa acaggtatta tctggtggat caagactagg tacttattat atggaaactg
    32161 gtagtggcga acgcgccgcc tcagtgatct atgatcgtgc cggatcaagt tttgctgaaa
    32221 tggatcattt ggaatggtcg ctagaggagt tattccaagg aatagatata tttcatgttt
    32281 caggaatcac cccagctttg tctaaaaaat ggcaaaaaat gacgaaacag gtgatagaag
    32341 ctgcaaaaaa agctggctgc ctgatcagct tcgatatcaa ctatcgtgga aaattatgga
    32401 cacagcaaga agccagcgaa gtaatccatc agcttttacc tttagtagat atctgctcag
    32461 ctggaaaaat ggatgctctt tatttgttag gtatcgagaa agcgccagaa gcagaagaac
    32521 aacctctaat ttattattat caaaaaatgc aggaaaagtt cccgaatatt caggtgtttt
    32581 attcgaccaa gcgtaccgtt cattcagcaa gtgaaaatga attgaagggg acattgtgga
    32641 tgaaccatga atatgtggaa tcccaagtcc atagaatcac cccaatcgtg gatcgagtag
    32701 gtggcggaga tgcttttgca ggtggcgtgc tccatgggat tttatccaat atgtctccgc
    32761 aagaaatcat tgattttgct acggcagctt ctgccttgaa gcatacgatc catggggact
    32821 gcaaccaatt tagtcaagaa gaagtcgcac aatttttagc atgcggctct ggaaaaatca
    32881 ttcgatagaa ggagtagaag aaaatgcaag aaatggaaac acgttataca cacagtccag
    32941 aagatatccg tcattattca acggaacaat tgagaaaaga atttttagta gaaaaggtat
    33001 ttgaaccagg gaaaatcagt ttgacttata cgcacaacga tcgaatgatc tttggtggag
    33061 tgacacctac tgaaaaagaa ttagaaatca cattagacaa agaactaggt gttgattact
    33121 tcttagagcg tcgtgaacta ggtgtgatca atatcggtgg accaggattc atcgagatcg
    33181 atgggcaaaa agaagaaatg aaaaaacaag acggatacta tatcggaaaa gaaacacgac
    33241 atgttgtatt ttcttctcaa gatgctgaaa acccagctaa attctatatt agttctgttc
    33301 cagctcatca taaatatcca aatgtaaaaa tcagtatcga ccaagtaaaa ccaatggaga
    33361 ctggtgaagg cctgacactg aatgaacgta aaatctacca atacatccat ccaaatgtat
    33421 gcgaaagctg tcaattacaa atgggttata cgattttaga accaggaagc gcttggaata
    33481 cgatgccttg tcatacacat gaacgtcgta tggaagctta tgtctacttt gattatgcca
    33541 atgaagatac acgtgtgttc cacatgatgg gcaaaccaga tgaaacaaaa catttagttg
    33601 tagacaatga acaagccatt atttcgccaa gctggtcgat ccattcaggt gtaggtacaa
    33661 gcaattactc attcatctgg gcaatgtgcg gagaaaatat cacctataca gacatggata
    33721 tggtgcaaat ggatcaatta aaataagcat aagggagaga aaatgatgga atttagtatg
    33781 gatatgtttc gtttagatgg aaaagtagca ttagtcaccg gtgctgtcta tggtattgga
    33841 tttgagattg cacgctcttt agctacagca ggagcaaaaa tcgtatttaa taacttgaat
    33901 caagaatcag ttgatgaagg tatagcgcat tataaagaag caggcatcga tgcaaaaggt
    33961 tatgtctgcg atgtgaccga tgaagaagcg gttcaagcga tggttcaaca gatcaaagaa
    34021 gatgtaggtt cagttgatat tctagtcaac aatgcaggga tcatcaaacg gacaccgatg
    34081 atcgaaatgg atgcggcaga cttccgccaa gtaatcgacg tggatttaaa tgccccattc
    34141 atcgtttcaa aagcagttat tccggatatg atcgaaaaag gcggcggtaa aatcatcaat
    34201 atctgctcca tgatgagtga attaggacgc gaaacggtca gtgcttatgc tgcggcaaaa
    34261 ggcggattaa aaatgctgac gaaaaacatc gcttctgaat acggtcaata caatatccaa
    34321 tgtaatggaa ttggaccagg ttatattgct acaccgcaaa ctgctccatt acgcgaaaca
    34381 caagaaaacg gtgaacgtca tccattcgat caatttatcg ttggacgtac gccagcagca
    34441 cgctggggcg agccaatcga tttggctgga ccatctgtct tcttggcttc tagcgcatct
    34501 gatttcgtta acggccatgt cttgtatgtg gacggaggca tacttgccta tatcggaaaa
    34561 caaccttaaa agagctttag tacactgttt agtggagagg ttgtgaaatc agcggattga
    34621 acgaataccg aaggacagct gattaaacat catttattcg aaaaaagagg ctgggacaga
    34681 agcatttaac tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa atttttgtga
    34741 atttcagctt atttccgaag gagttgcttt tacttccgcc gtttgtacgt gtttagagcg
    34801 tgagacaaaa gtgttcttta cttttgtccc acgctctttt ctgtgcgtct tccatacttc
    34861 actaagtcga tccctcttgc ttcgcaccaa tccttcactg gtccggaaaa gatcagagat
    34921 tgttggtgta gggccgcggt attcgttgcg ccgaccatag tataaagcag acgaagctct
    34981 tcttgccatt gcttcatcag tataatagtt tcttctacgc cgtgattcat cagatgcgtc
    35041 agtacggttc ctgaagtacc cacggcattt gcgccgagac aaagggcttt aaatatgtca
    35101 taggcattgc ggatacctcc ggaggctagg atttccatag aagtgtctgc ttcatttgct
    35161 tcaagcaatg aagagactgt cgattgcccc cagtcagcta aataactcag ttcccgcttg
    35221 gagcgacggg cgttttcgat ctgggtgaag cttgttccgc ttcgaccgct gatatcaact
    35281 gtatgaactc ctaaagcagc tagatcattg agcgtttctc ttgtcatgcc aaaaccgact
    35341 tcttttacga tgagggggac gtcaatggct gtttgagttt cttgaataag tactttccaa
    35401 ttggtaaaat cacggtctcc ctcaggcata accaactctt gaggtgcatt caaatgaatc
    35461 tgtaaggcat ctgcgtgaaa caatcggata gcttctttgg ctctttcgac agaagtccct
    35521 gcaccgatgt tggcaatgat tttcccgtga ggatattctt gtcgcataat cgtataagta
    35581 tcggctaaag aagggacttt caaggcagcg ctgactgaac cagtggcaat catcaaatct
    35641 gcttctcttg cgacgattgc aagatcttgg ttgatttttt ttgttttttc gcttccgcca
    35701 gtcatcgcat tgatataaaa tgggctgctt agtgtaaacc caactgcctg tgtacttaga
    35761 tcgacatcag aaactgctat ttgcggcaaa gggttatgga tgatccgtac gaagtcaaat
    35821 tcgttttttt gtttatcatg aaaggcttta gctagtgaaa catgttcgtc ttttcgattc
    35881 atcggttttc ctttctttga tcgctataga catgtaacgg cagtggagtg atttctgctt
    35941 tttcccatgc actcattaaa ggaagaatgc cagatttctg gtcaacaatt acgattccgc
    36001 aatcgccccc acctgcacca gaagattttg cggctccttc atactgttca gctaaattac
    36061 acaatttgtt caaagcaggc gtttcgatga cgaccccagt gattgcagat aaatcatgca
    36121 gtaattgtcg gttttttcga atcatctgtt ggatcaacgt tacattattt tctttaaacc
    36181 ctttgatcat ttcgttgaca cattctgtac tattttttaa gaaaagctga taagccacca
    36241 ttttatcttc tctcgaacgg tgaacttgat cgaccaaatc agaagtagag gcagggctac
    36301 ccgtccaacc aatcaataaa cgtaaatctt caggagcaat caatggctca atggatagac
    36361 ctggccaatc cagggcaagt aactcactga tagaatgctg agtttcttgt tcttggagcc
    36421 aaggatggtc gaaggttgag aaagcgatcc agccaccata acagctagca gcgatgtcgc
    36481 cgcaggaacc attatcttga actgctaaat tggctagtgc tgcaatcttg aaaatctcca
    36541 actgagataa attcaacgcg taaaaaacat tcaaggcttt gaccgttgca acggtcacgg
    36601 caccgctaga acccaatcca tatttccggc catttgaact gtctaactca cttgttactt
    36661 tcagatcata aaaggataaa agaatgtttt tttcttgtgc atacttttca gtcaagcgaa
    36721 tagcagcaag gatataatga aaaggatttt cccgaatatc taaaactaat tctccgttgc
    36781 gtcttgtcca acgtacaggc atcccactat attgtgcaga ttggatactt ccgacttttc
    36841 gtgcagattc tacagtgact gtcacgaatt gatcgactgc agcgataact gctggatggc
    36901 ctgtttcaac aactgcatat tctccggcaa tatagagttt gcctggtgca gatacttcaa
    36961 tcataaaaat ctccctttat tcaataatcg caattcctgg accagcatgt gcagtaatta
    37021 cttgttcttt gctaaataat ttggtaaatg tttcttgtac ctcagataaa tggctgtttt
    37081 ccactaaaac tttgacatta gggccagcat ccatcgtaaa gtagcatgga atgccttgct
    37141 ttctcaattg tcgcaccgca tccatcgctt ttaagctgtc aggagaccag taagtgaatg
    37201 gaggctgggc agctaaagtc gttccatgca tttttagtcc gtttctttcc atcgtttctc
    37261 cgagaagttg gaaatctttt gttttgattg cttgtttcaa ttgatataga tccccttcaa
    37321 cggaatctaa ccagccttga taaaaactgg atgtttcgac tgtccgacgc atcccatttc
    37381 tgctggacac ttcttttttc tggtcgttga tcaaaacgaa aaccattgct aaatcgtctt
    37441 cgaaagaatc ggaagggact ggcttagcgt aagaacttaa gtcgtcatgg cctttttccc
    37501 attcgacgaa accaccgaaa atacttcggc aagctgaacc agacccgcgt cgagcaaatc
    37561 ttgaaagaga gagatcatct aatccaagct taagagcagt attgcaagcg cctgctaaag
    37621 cagctagccc gctggcagag gaggctaatc cagctgcggt aggaacgaaa ttctgactga
    37681 ttactgaagc ttttagtgag aggccggctt ctttacgaaa aaggtcaaga aactgactga
    37741 ctttttttgt tgctttttcg tcttgcaagg tgccatctag ataaaattca tctaccatat
    37801 agctatcaga aaagatgact tctgtctctg tataaaatgc atccaatgtt aacgaaaggc
    37861 tgttgttcat tggaaggatg agttcttcat ttttcttacc ccaatatttg attagagcaa
    37921 tgttggtata tgcgcgtgct ttgcctttaa acataggtat gtactcctaa tccttgtatc
    37981 caagtttcag cagcacccgc atcttcaagt gcttgactga tttcttgggc ttttgttttt
    38041 gtttgtgcta atgcaatcat acagccaccg cgtccaccgc cagttaattt ggcacccaat
    38101 gccccggtat cttttgctgt ttggatcaat agattaagaa aatcattgct gattgtcagc
    38161 ttttccagaa gtgattgtga ttcatccatt gtctgtgcca acgtttctgg tgaattttca
    38221 atgattgctt gttttgcttg cttcgtcaag tatcctaatt tttggatcat ttgtccagtt
    38281 tcatgaggct gggtttcaaa gaggtgagcg acatctttga cggcttctct tgtttgtcct
    38341 ttgattcctg tatcagcgac aatcaaaaaa gcatcgatgt tcaaagaaaa gtagtcgaaa
    38401 ggatgccctt ttgtaaaata aatcggctgc aagctgctag tagcggctgc atcgattcca
    38461 ctaggattac cgtgggcgat tttttccgaa agctggacat tttctaatag tatttcacga
    38521 gacaaaggaa atgctaagta atcataaaaa gcacgagtga ctgctgtggc gactgcagcg
    38581 cttgatccca ttccacgttc agcaggaatc gtgctttcaa tggtcagttg caagtttttt
    38641 tggatgtcat gctgtttttt taagtgaatg aataattttt tgatattttt taatgcatga
    38701 ggagcttcgg ctaaatttcc tgaatagtag gaagaaacca gttgatcgtc cattgcatcc
    38761 agctcttcaa ggaatgcggt gacttttgtt gcatagaaag gaaaggcaat cgctggttct
    38821 ccataaacaa ccgcgtgctc gcccatcaat atgatctttc cgcttgattc tccttggcca
    38881 tagtttgcca taatctaact ccttctcacg taaaagtgat ttttaagcaa aaaataaagc
    38941 gcccaaaacg agttgcgctg cttcacgaat ttttttgtcc actaagggtc tcaaaaaaca
    39001 ttcctccttt attttactaa aaccatctca gaagtaaaac taagaatacg ttttctaaag
    39061 aaaaaacact tttttcttaa gaattttttg actttttttc ttctttgact agaaaaaaat
    39121 acaattgaca ctactagata ttgggggtat atttaaaaag gttggagtta aacacaatat
    39181 ttagtgatga agtgagctgg aggtaaatga gcgtgatcgt tttagcaggt acaattggtg
    39241 ccggaaaatc aagtttaaca gaaatgatgg ctgaacactt tgatagtcag gcattttatg
    39301 aatcgattga tgataatgaa gtcttgccct tattttatgc aaaccctgaa cagtatgctt
    39361 ttttgctgca aatttatttt ttgaataaac gttttgcaag cataaaacaa gcaatgaaag
    39421 atgacaataa tgtattggac cgttcgattt atgaagattc actgcttttc catctaaatg
    39481 cagatctagg aagagcaaca gaaacagaag ttcgtgtgta tgatgaactg ctggaaaaca
    39541 tgatggaaga acttccctat gctgcccata aaaaacatcc ggatttatta gttcatattc
    39601 gtgtatcttt tgatacgatg ttggagcgta tcgaaaaacg tggtcgttcg tatgaacaat
    39661 tgtcctttga tccaagtcta tacgattatt acaaagaatt gaacagacgt tacgatcaat
    39721 ggtatgaaga atacaaagaa agtccaaaaa tccaaatcga tggagaccgc tataattttg
    39781 tggaagatcc tcaagcaaaa gaagaagtct tgaaaatgat cgaagaaaaa ttagcagaga
    39841 ttcgccaaac caaagtagcc atttgattta cagttgctca aataaagggt aaacctttat
    39901 ttgagcgact tttttcttta tactggaaac aaatgtcagg aggatagagt atgcgggaaa
    39961 aacactattt taatcaagac cgtaattttt ggatcaatgt acagcaaaac gatgtgctag
    40021 ctgttaaaaa cttaaaagaa gaatatggca ttagtgacga gatgctgact tattctctag
    40081 ataaaaatga acgggcgcgc gtagagtacg attcttttga tgaagcgtta ctgcttgtat
    40141 caaatgttcc gcaccagcaa aaagtcgata atcattatga aactagtcct atcgctttta
    40201 ttttaaaaga agacggtttg tttacattta ccacgccgaa taccgagtac gtgattcgac
    40261 taattcgatc gcttttggaa cgaatgccag acatgagtgt atacagttta ttgtttcgta
    40321 cattgttttt gatttctgat tccttttttc ctttaatcga ggaagtgaat agtgaacgtc
    40381 agcggcttaa tctgaaatta agagaaaaaa caacgaacaa gaatctgctg caactttcag
    40441 atttagagat cggtttggtt tacttagtta caggaacaaa acaaaatgta gtacttttag
    40501 aacaaatcaa ggcattggct atttatcgca agttgtcaga aaaggaaaaa gaagagttgg
    40561 acgatgcgct gatcgaagca cgacaagcag ttgaaatgac gaacttagct tcgcagattt
    40621 tagaccagct ctctggcact tataataatt tgctgaataa caacttgaat gacacgatga
    40681 aatttttgac ggtctggtcg ctgatcttga ccgttccaac tattgtgaca ggattcttcg
    40741 gaatgaatct ccagttaccc tttacccatt ccgttttcgg atggggaatc gccttgatca
    40801 tcagtttggt cttatctatt tggatgctta ttgcactgtg gcggcggatt cgttgatcta
    40861 gtctttgatc tgcaaaccaa gggcttgggc gatcacaagt gcttgctctt ggttgacgat
    40921 tagtcccgcc aaagaatctt gagataaagc gattgctgtg aagaaattag tcgtaaagtc
    40981 taatcccttc agagaagttc gaaaccagtt cgaaccggtc aactgattgt cagacaaaaa
    41041 aaggtttttc caagtgacct cataaaattc gctgtcttct aattgacaat gatcgaagga
    41101 gacagctttc aagtttgtac tactaaagga agccatcgaa gcaatacaat cagtgaaagt
    41161 acagtctcga agataacttt cggcaaaatt cgtacctgtt aatttacatt gatgaaacac
    41221 gacttggtgg aaactgccac caatccactc cagattggaa aaatcacatt tttcaaaaat
    41281 gacattactg cattcaaaat gacgtaaaac tgttttttgc atcgtaagtt tttggatatg
    41341 actctctctt aataccatat ttttggcatc gagatagctt tggtcacccc cgttgatcag
    41401 aagaccagag aagatctcct catcttctaa ataaaactct ccctttgtaa gaagaggaag
    41461 agaaggtgga ataggttcga tttttttcat gtgggttcac ctcgtttttc tttagtatag
    41521 caaagaagcg ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag
    41581 acaaactgta tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag
    41641 aaataaaagc agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt
    41701 tttcactggt tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat
    41761 tgccgtttat tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc
    41821 aaaggatatg atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga
    41881 tggtgagcaa ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa
    41941 cttggatctt gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta
    42001 cggtaaatac cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt
    42061 gatcaatgta aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa
    42121 acttcgtaat gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt
    42181 caaaggccgt gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga
    42241 agaaactgcg gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt
    42301 cttgacgctg gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac
    42361 agccgatagt cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa
    42421 acacaccgcg gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc
    42481 cgcgcgttta caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt
    42541 aaagcaagca tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg
    42601 aaataagaag tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat
    42661 ggcacgtgtt aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc
    42721 aaaaggttac tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa
    42781 ttcatacaac tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg
    42841 gattgctcgt atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca
    42901 cggtttgaaa gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa
    42961 cgatgcagca gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc
    43021 taaacaatta taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat
    43081 ttacaatcct ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta
    43141 aggtttttta attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca
    43201 taagttagaa tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg
    43261 aggaataaca tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg
    43321 gtctggtact gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta
    43381 tggatgggat tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa
    43441 ggagtaattg atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg
    43501 atcttggtaa atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc
    43561 gcagctaccc tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg
    43621 agcttactat gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg
    43681 atttctattg cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt
    43741 gcactgttct catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc
    43801 aaacaatatc aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat
    43861 gactatttcc aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc
    43921 gctaatatat caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa
    43981 ggtggcgacg caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt
    44041 cttttagatc ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt
    44101 actggaaaat tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca
    44161 gatggcgcag tagatgttgc gccaatggtt ggtttcttga tgaccttagc gatgttcaat
    44221 aattcagctg cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa
    44281 tcaccattag tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt
    44341 ccaatgaatc tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca
    44401 acgatgtcgc cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat
    44461 ttagatatta cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga
    44521 gaatatatga agaaatccat cccaactggt tggatcatcg gagcgatctt atgcctgatc
    44581 acgttcttat tgtacgggaa cgcctagtaa acaaaacgtc gataaaaacg attagatgaa
    44641 tggaggaaaa gaaaatgaaa cgttttgtgc gaatgggaat tgatgttggc ggtacacata
    44701 cgaaagcagt cgcaatcgat aatgcgacgc acgaaatcat cggaaaatct tcagtgaaaa
    44761 caacccacga tgatgtccgt ggagtagccg caggtgtcgt tcagtctttt caaaattgtt
    44821 tgctggaaaa taatatcagt ccagaagatg ttgtgtttgt tgcacattct accacacaag
    44881 cgactaatgc actgattgaa ggagacgtag caaaagtcgg cgtaatcggg atggcaaaag
    44941 gtggattaga aggtttttta gcaaaacgac aaactagatt gaacgatatc gatttaggga
    45001 ataagaaaaa aatagagatt gtcaatgcat tcttacctgt caaacactta aatgtagacc
    45061 gtgtttcaga aacgatttct tctttagaaa gagaaagagc ggaggtcttg gtttcttcta
    45121 tggcatttgg agtagataat ggggaaccag aacgagttgt ttatgaagca gcatcggtaa
    45181 aatcgatccc tacaacgatg gcttctgaca tcacaaaatt atatggattg acaagaagaa
    45241 ctcggacagc agcaatcaat gcatctattt taccaaaaat gctagatacg gcaacctcta
    45301 cagaagattc cgtacgagaa gcaggggtca atgtttcttt gatgattatg cgcggagacg
    45361 gcggagtaat ggaaatcaat gaaatgaaaa aacgacctgt tttaaccatg ctttccggtc
    45421 cagcagcatc agtcatgggc tctttgatgt acttgagagc gtctaatggt gtttattttg
    45481 aagtaggcgg aacaacaacc aatatcgggg tcattaaaaa tggacgtcca gcaatcgatt
    45541 attcaattgt aggtggccac ccaacatata tctcttcatt agatgttcgg gttctaggtg
    45601 tggcaggcgg ttcgatggta cgggctaatc agtcaggaat catcgatgtc ggtccacgtt
    45661 ctgcacatat tgccggtcta gattatgcag tatttacgga gacagaaaaa atcaaaggtc
    45721 ctaaggttga atttttctct cctaaagaag gagaccctgc tgattacgtc aaggtagtga
    45781 tggaagacgg agaagaagtg actattacaa atacatgtgc agccaatgtc cttggattag
    45841 tgcaagaaga acatttttct tatggaaatg tcccatctgc acgaaaagcc atccaagcat
    45901 tagctgatta ttgtcatacg acggttgaag acatcgcaga acagatcatg gaaaaatcct
    45961 atgctaagat tgaaccggtg attttagaat tggcagacaa gtatcacttg gagaaagatc
    46021 agatttctct tgtaggtgta gggggcggcg ctgcctcatt gattacttac tttagtaata
    46081 aaatgggtgt gaaatattcg attcctgaaa acgccgaagt gatttcttct attggtgtag
    46141 cacttgcaat ggtccgagat gttgtagaac gtatcatccc ttcgccaagt aaagaagata
    46201 tacgatcgtt gaaaaatgaa gcgatgaata aagcgattga atcaggcgct acaccagaat
    46261 cgatcgaagt ccacgtagaa atcgatccac agacttcaaa agtaacggct atcgctactg
    46321 gttctacgga agtgaaagca accgatctaa ccaaggagat cacaacagaa gaagcactag
    46381 agcttgctgc agaggatatg cgattaaata aaaatgaagt ttgcttattg gaaaatacac
    46441 ctttctttta tgtatgcggc gaacagaatc gttcaaaaaa tgctggaagt cttcggatca
    46501 tcgatcaaaa aggatttatc aaggtccaac gtggacatgc ttcatgtatg aaaacaactg
    46561 cagccaatta tatgacagct gtcgaacaac tttgggaaga tatggcagtt tatcaaactg
    46621 aattgattgc ccgaccagaa ttttatctct gcttaggagc aagagtctct gattttacag
    46681 cgacggatct ggaacaacta cagcttttga tggatctgga agtatctact atggaaccgg
    46741 aggaagaagt gatcgtcgtt gccggaaata tcaaacaaac ataagttaga agagttacta
    46801 aacgaattac aacagatccc cgaagaaatt tggggattct accagtttcg gcgagattta
    46861 ttttggaaaa agattccttt gtccaagcag aaaattctta tacaacaatc aatcgattgt
    46921 ggtatcgaaa cggcatgtag catcaaaaaa aagtaccctt ttgccgatgt aggagaaatt
    46981 tgtgaacaaa tggctgttcc aatcgtttcc tgtgaatcag agcaaataaa cgaacgaatc
    47041 acttttgcaa cttatgcaga agatgaaggg attcgattga tgacggagcc gttagaaaaa
    47101 ttaaaatgtt caggactagc ttcgatctct aaagaaacag cgcaagcttt gattattgga
    47161 catgaactat ttcaccacat cgaagcttct gttaagggaa tttatacgca aagcgaaaaa
    47221 attgtgctgt ggcggttgcc tttttataca catcaatcaa acattcgtgc attgagcgag
    47281 atagcagcga tgagtttttc aaaagagatg aatcaaagcg atttttcgcc ttatgtatta
    47341 gaggcagtct tactttggcc atataatgaa attcgtagcc aagggatctt agaagaaata
    47401 aaaaaaatag aaaaaagatg tacggagtat gatttagatt acaaataaag agactgtgac
    47461 aaaagtcgtg tcacagtcct ttttttccga ataaacggtg ttctcctgtc aactcctcgg
    47521 tcacaagtca aaatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac
    47581 tcggagatga acggcaggct cacgacctct tggaaaacgg tgttcaaaag cttgcatctg
    47641 cggtaataag tccagccaag ttaaaaacat aagaagctat ttttacttgg ctgttcttat
    47701 tacttgatgc agggcagctt tttcacaacc tctcaataaa cggtgtgcaa tcagctgacc
    47761 ctcggtatta gctcaaaaaa aacggcaaaa tatgaggaac tattttgccg tttctttctc
    47821 taatactcag gtcaaaacgc tgattccaaa acctcttatt tgatgggtct gtcctgaata
    47881 tcttcaaaat aatcggggta attatgaatc acgtattctt gctcctcgat aatcatatgt
    47941 aaatgcatcg tgcaaagaaa tccgacttca tcaaagttat gatcaatcat tgattgaagc
    48001 attgtctgat gttcatctaa tactcgaccc cagtctaatt ctgaaatcgt caaacgtaac
    48061 catctgaaac ggttcaagtg cgtactataa ctttctagcc aatcccaaat ttctcgtttt
    48121 ccagcaattt cataacaagt tcggtgaaaa tcagtatcca aatcaaaaaa agctttttta
    48181 ttttgagcga ttactgcctg atcggtttgt tctagtattt tttctagttt agcttgatct
    48241 tgcttggtca ttttcgcact gcattcttgc aggatcggtt tttccaactt ttcccgcgta
    48301 taacgggcta atgaagcaga tcggatatcg atttttgaaa cataagtgcc ggactgtgga
    48361 atggttttta caagctcttg attgcgcagt tggatcagtg cttctcgaat cggtgtacgc
    48421 ctgatagcta gttgttcttc taacacttta tcggagattt tacttccagg tgctaactcg
    48481 gaataaatga tttgtttacg tatagattca taagcttgct gttgcaaatt attgataacc
    48541 atttaacctt cctttctatg cagatataat ctattataac ataaatcgct tacaaatcgt
    48601 gtgggcataa aaacacagtc tgcaaaaaaa gccctgatag gcatgcctat tagagcctcg
    48661 aaaagaaaaa tattgaatca atctggtcgt acctcttgga tcggtttgac acgaccgccg
    48721 attcttttag caaaagcttc ggattctttt ttgtctttga aaatgagcag attgtctaag
    48781 tttttttccc gagtgcgaca gccagtttta tcgatgtaac cgtgcattac tttaacgaca
    48841 tacatgggga tcacctgctt ttttcttttc attatacgaa ctactcgaca caatacaaga
    48901 aaaatgctta gacggtcttt attaagaatt tacatcatcc aaagaagcgt acgcttggaa
    48961 taactgatct aactccggat tgactttgct cagcttcact aatcgttgcg aatcagaaag
    49021 taaaaaacag tgtttactac tgccagtagt ttttaatttt ttgtcttcag ttcccacaat
    49081 ttcgtagcta aaatccaacc gtgtccccgt atacttttgg atagttggat ggatgctcac
    49141 tttttctccg taatggatca tttctttata gctgcaattt acttccagaa cggggatgat
    49201 gatcccagat tcttcgattt ttttataggg aaaccctaga tgtaccaata aggctacccg
    49261 agcctcttca aaccagcgga tataattgct gtgatgaata atccccattt gatctgtttc
    49321 ataataaaaa ggttctcgca cataacctgt aaatttctcc atgactcatg cctcgttttc
    49381 cattgatttt ttacaaaaat aattcatcca tttcggaagt gcttcgctga tttgtgtcgg
    49441 caaaacaata tattgttctt ttgccagctg gtctccaatg aaactatgaa gataaatagc
    49501 tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga tcatcccagc
    49561 taaagtgtca cccatgccgc ccgttgccat agcagcagaa cctaaaggat tggtgtagaa
    49621 gtgctggtga ccataaattt ttgtctcatg acttttcaaa acgagcacag caccgatttc
    49681 ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt atcggtagag aactcaaccg
    49741 ttgccactcc atttgatgag gtgtaagtac agtttgttcg ggatagggta gcaaaagatg
    49801 attagcagca agtaaactta aagcagagcc atcaatcacc agccattgtt cttttgattg
    49861 gttttctagt acttgtttga aacgttccaa actcttagaa gaagtaccca atccgggacc
    49921 aatcaagagt acatctgctt gctttattac ggaaagaaac tggtcagtat ccgtccaaac
    49981 tacaatcatt gcttctggca gatgactgtg aagagcactc cgattttttt catcagttac
    50041 tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa gcactcataa tgattgctcc
    50101 gccatattgt tcattgccgc caatcaaaac gatacgtcca aatgttcctt tatgtgaaat
    50161 ttttggacga ggtgtgatga ctcttttcac tagttgttcg tttatcagca tctaaaaacc
    50221 tccttttact cattatagcg tatatttttt ttagagaaaa acagataaga aaaggaattg
    50281 tcagattttt tgaactattt ttttctgtaa tttgtggtat catgaacacg aagacttaaa
    50341 taagtggagg attaacttta tgacaattga ttggcaaaaa gaagtagaag cacgtaaaga
    50401 agacttgctt gaagacttga aaaatctttt acgtgtcaac agcgagcgcg atgactcaaa
    50461 agtaacacca gatgcaccat ttggacctgg acctcgcgac gcattgaaac atatgttagc
    50521 ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag ggcatattga
    50581 tcttggcgaa ggagacgaaa cgctagggat atttggacac atggatgtcg ttcctgcagg
    50641 agatggctgg gacacagatc cttatgaacc agtaatcaag gatggaaaga tctttgcacg
    50701 cggatcaagc gatgacaaag gtccaagtat ggcagcttac tatgcgatga agatcattaa
    50761 agaacttgac ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg atgaagaaag
    50821 cggctggggc gatatggctt attatttcga acatgaagaa gaaccagatt ttggtttctc
    50881 accagatgct gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt tagctttacg
    50941 cttcaaaggc gacaatgcag gagattatgt cttgaaatct tttgtttccg gacttcgcga
    51001 aaatatggtg ccaggaacag caactgcagc acttcaagta ccaagtgcag atgcagctat
    51061 cgcgatggaa gaagcatttt accaattcat cgaagcaaat ccagtaagcg gaacgattga
    51121 agcggacaat acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg gtgcaagccc
    51181 acaatctggt atcaatgcag gttctttctt ggcattgttc ttagataact atgaattcct
    51241 aggatctgct aaacaattta tccatgtagc agctgcttat gtccatgaag atttttatgg
    51301 agaaaaacta ggagtcgctt atgaagatga aaaaatgggc aaattgacaa tgaatgctgg
    51361 actatttgct tttgaagaaa atggtacaga ggaagcgaac ttcatcaata tgaatttccg
    51421 ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa cagtggggca
    51481 agaaggcgca acagttactc gaggtgctcg cgtaatggaa ccacattacg taccaatgga
    51541 tgatccattg gtggctacat tattgcaagt ctatgaagac cacactggag aaaaaggcta
    51601 cgaacaaatc atcggtggcg gaacttacgg acgtttgttg aaacgtggcg ttgcttatgg
    51661 agcgatgttc ccaggatata ctgatacaat gcatcaagca aacgaattta tgagtcttga
    51721 tgacttgttc agagcaacag caatctatgc agatgccatc tatcgtttgg cgaaataatt
    51781 aattaaaaag aggatcaaga attgcaaatt atttttgcag tcttgatcct ttttttactt
    51841 tctactgtgg tattcatgat aaacatgata tagattcaat tcatattctt tcttagcgtt
    51901 cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta aagtcatcaa
    51961 tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca tatattcgcg
    52021 aatcacagga atccccgtga ccaatccaaa taaaaagaag aagatggtcc aaatcccaaa
    52081 gaaaagcaaa ggcttataat ctttaaacat tttaataatc atcatgatca ctttgaatcc
    52141 gtcagagaat gtattcaatt ttgattcgct gccttcagga cgatcacgat aatcgattgg
    52201 gatttcaacg aacttgaatt ttttatctaa agcatggatc gtcaattccg tttcgatttg
    52261 aaaaccagaa ctcataatag gaaaactttt gacaaagata cgattgaatc ctcgatagcc
    52321 ggtcatgaca tcaagaattt ttgttttata cattctagta attgtatttc taacaaggtt
    52381 atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag taggttccat ttgataggcg
    52441 atcaccaatg accatatctg cttcaccaga acgtaatttc tcgagcaaac catgtacagc
    52501 ttctgcagga taagtatcat cgccatctac cattaaataa tagtcggcat cgatatcaaa
    52561 gaacatttgg cggatcacat ttccttttcc ttgacgaggt tcttttttta cgattgctcc
    52621 accttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga cataaatatc
    52681 tgcttctggt aattctcgtt taaagtctgc aatgactgtt gaaattgttg cttcctcatt
    52741 gtaacaagga atcagaacag caattttttc ttgttgttcc atttttcact ctccttaact
    52801 atagaaaaca aaagctaaat taaaaatacc tcataatcaa aggaaatgct agtttttccc
    52861 gctaaatatc atttatttaa aaagaaaata ctggaatata tgtttggaaa gctctatcta
    52921 aaaaaccacc cactacaaaa gaaggacatg atacctctct tttgaatagt gggtgcgtat
    52981 tcgttctctc acttttttga cgtattaatc tgttgcacct ttttttcttg aaatttctaa
    53041 tacggttcct gtatgagcat ctgctaaaaa ttcataataa acagtttcgc catcttctat
    53101 tcgagaaaga cctccccgat aagcactcga ccgaacggcg aatttcctaa taggttcctt
    53161 ttcaaaagag atccaagaac cttcgattgc accttctttt agaaaagatg acttgatatt
    53221 ttcaagtact tggtctgctg acaatacttt tttgcgatga taccaaatcg ttgaagctac
    53281 cccgctcaat aatcctagac cgattccaac tgctaacccg cctttaaaat agcttagttc
    53341 gctttcttca tacatgtgga catacctcct tcttctcact tgttactcct tgtgcttatt
    53401 ttaccacact ctcatatttt ctttgaaata aataaccgct accagaaaaa ataagctata
    53461 ataaatacga tcggtctgca gaaaaaatca tattttatcc gcagatcaat ataagtctga
    53521 gaggagttta gtctgaaatt tagacgaata gcatatggaa gaaaaaacat ttcaacgaat
    53581 caaagagtta acagaattac aaggaacaag cggttttgaa caagatgttc gcgcttatat
    53641 ggaaagtcat atggaaccgc tggtagatga gttacagtta gatggtttgg gcggcatttt
    53701 tggtctgcgc catcatgaag aagcggatgc accccgtgta atggtcgcag cacatatgga
    53761 tgaagtcgga ttcatgctga cacaaatcca agaaaacggg ctttttaaag tcgtacctct
    53821 aggcggctgg aatccttacg ttgtttctgc tcagcgtttt acacttaaaa cctctactgg
    53881 taagaattat ccatgtattt cttcttccgt tcccccacat ttattgcgag gaacaagcgg
    53941 acaaaaatca gtagaagtaa ccgatatttt gttcgatgct gggtttgaat caagagaaga
    54001 ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg aaacgatcaa
    54061 aacagccaac ggaaagaata ttatcagtaa atcatgggac aaccgctatg gctgtacaat
    54121 ggtattagaa gctttagaag cgttgcaaaa tgaaacatta ggtcatacat tgattgcagg
    54181 cgcgaatgtc caggaagaag taggcttgcg tggttctaaa gcttctgtca ataaattcaa
    54241 gccagatctg ttctttgcag tagactgctc tgcagcagat gatacagtga cgaaaaatgg
    54301 cacattcgga catttaggag aaggtacatt gatgcgtatc caagatccag gtttgatcat
    54361 gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa acaaacaata tcccttatca
    54421 atactttgtt tcaaaaggcg gtacagatgc aggtgctgct catacacaaa acgaaggaat
    54481 cccaagtaca gtgattggcg tagtgggacg ttatatccat acgcatcaaa cgatgttcag
    54541 tattcgcgat tttgaagctg cacgagaaat gttgattcaa acactgaaag gtttggataa
    54601 atcaacagta aatacgatcg tatacggaaa ataatgcgat agtgaaacta taaaatctgg
    54661 caaaagattg ctttttgcca gatttttttt ataaaaagag gatcaatgtt tgttagttcg
    54721 aaagaaaggt gtataattta attgaaaaat acataaacag aaagtagagg acaaagaatg
    54781 attatcccaa atagtttaga agaattagca ggctacgtag aaactggaaa gagtgtattt
    54841 ttctttactg ccggctggtg cggagactgc cgttttatca aaccgcaaat gcctgaaatt
    54901 gaaaatgact tttctgattg gcaatttatt gaagtagacc gtgacaagta catagatgta
    54961 gccgctgaat ggaacatttt tggtatccca agttttgtcg tgatccaaga cggaaaagaa
    55021 ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc gttcctagaa
    55081 agtgtaacaa aaggctgaca tctgttagga ggggtcaaga tgaatcaaga atataagaat
    55141 attttagtag gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa agcggtagaa
    55201 gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg taatcgatca gcaattttat
    55261 aactttatgg gctatgcacc tattgatcaa aatctagtcg atcagcaaac tgaaacagcc
    55321 aaggaaatga tcgaagaatg taaagaatat gggaaatctg ttgattacac aaatatagaa
    55381 ggaattattg catatggttc tgttcgtgaa tccatggcag tcaaattgcc aaaaaaatat
    55441 gagatcgatc taatcatggt tgggcagtca ggtctaaatg cagtcgaacg ctttatgaca
    55501 ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg atgtcttgat cgtttctgac
    55561 gaagaagaac agccaagtaa ttaagcaagt ggaacttgta cgaccttttt aatcaattaa
    55621 ctaaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta cgaataaatg
    55681 gtgttccaga agaggcttct tcggaaataa agcatgaaaa aaatttcatg cagtgaatgc
    55741 aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat gatttttgct
    55801 tataacaaag cacacgtcgg agacaccttg atggttatta tagccgacga taaaggaaca
    55861 gaaaacactg ttgaacgcaa atggaatgtt gctcggatca aagatgaaca aggtcaagtt
    55921 gttgcttgga actttttcca tatctccgat catctaacaa tcgaagggaa cggccaagta
    55981 agcttaacag atgaggaatt agcgaaattg aatcgattga tcaaagaagc aggattcgaa
    56041 gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa aacatgcgtc
    56101 ccacatcctg attctgacca tttatcgatt actgagacag aagtagacaa tggtacagta
    56161 ttgcaaatcg tttgcggtgc acccaatatc gaagctggac aaaaagttgt cgttgcaaaa
    56221 ccaggagcta tgatgccaga tggaatgatg atctggccag gaactttgcg aggggtagat
    56281 agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc agcaaaaaaa
    56341 ggaatcttag tattgcctga ggatgccgta gtcggagaag catttccaaa ataaagaggt
    56401 tgtgacaagt ctttgtcaca acctcttttt tattaatttt cactattttg attttgtttt
    56461 aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga gccttcatag
    56521 taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg aagttctaca
    56581 ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt caatccagct
    56641 ttttcagccg gtgtagctgt ttggacattt gtcacgatga caccgttagt tacagatgaa
    56701 ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc gaccattgtg
    56761 atacctagag ctggacgtgt cacttttccg tctttttcca gttgattgat gatattcact
    56821 acatcgttac ttggaatagc aaatcccatt ccttctacgc ttacattaga agtagattca
    56881 gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat attaactaaa
    56941 ggaccaccag aatttccagg gttgattgct gcatctgttt ggatcgcatt gatattgact
    57001 gtttcgttcg attcattcgt acttgtcact tgtcggttca aggaagaaat gattcctgaa
    57061 gtgacagaat ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc aggctcacca
    57121 accttcagag cacttgagtc accaaaagag gcaactgttt cgactttgtc tgaattgatc
    57181 ttcaacaccg ctaaatctga ataagcatct gttccaacta attcagcttt taccttcgta
    57241 ccgtctttca tcaagacttc aagaccttgc tggccatcta ctacgtgatt attcgtgact
    57301 acataagcag tattcccgtc ttttttgtag atcacgccgc ttccttcact ataagcttcc
    57361 aaattactat catctgagct gctactttct tcttgctgcc cgaatagtcc gccaaaaccg
    57421 cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg aactttatct
    57481 accgctttgg tgatatcaga atctacattg actttgacat tttccacaac tgtttcaccg
    57541 gcagaattct gattgccgct tgtagcagta gaactattgt tgccagtgcc catcgctgca
    57601 taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc cagacctaac
    57661 ttacgccaga ggctattatt tttacttttt ttcatctttg gtgtcacatt ttttctatcc
    57721 atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt atttgttgac
    57781 tttagtctac tttgagactg tgaacttttt atgaaaaact ttaggaaaaa tcattgtttt
    57841 cagatgaaaa tatttgattt tctttttcca cagatactgt gtaaaactac gcggatagga
    57901 ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat aaattcttat
    57961 aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaataacact gttttctgtg
    58021 gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa aaatagccaa
    58081 aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcatga agaccaaagg
    58141 agagaaaaca tgaatatcaa aattatctca gtaggaaaat taaaagaaaa atacctgatc
    58201 caaggtatca atgagtatgt caaacggctg aatgcctatg caaaaatcga attgatcgaa
    58261 gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca agtaaaagaa
    58321 aaagaagggg aacggatctt agcgaaaatc aaagaacaag aatacgtcta tgccttagca
    58381 attgaaggga aaaatcctac aagtgaatct tttgctaaac aaatcgacca attaggtatc
    58441 caaggaaaaa gccaccttgt ttttgttatc ggcggctctt taggactcag cgaagccgtc
    58501 atgaaaagaa gcaatgctca gatctctttt ggaaaaatga cgtatccaca tcaattgatg
    58561 cgtctgatcc ttgtcgagca aatctaccgt gccttcc
//
LOCUS       DACDHJ010000002        57990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDHJ010000002 DACDHJ010000000
VERSION     DACDHJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.002"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..1957
                     /gene="tdc"
                     /locus_tag="IV223_000060"
     CDS             80..1957
                     /gene="tdc"
                     /locus_tag="IV223_000060"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8710375.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2200..3621
                     /locus_tag="IV223_000061"
     CDS             2200..3621
                     /locus_tag="IV223_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8710376.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3730..5097
                     /gene="nhaC"
                     /locus_tag="IV223_000062"
     CDS             3730..5097
                     /gene="nhaC"
                     /locus_tag="IV223_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8710377.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(5378..6397)
                     /locus_tag="IV223_000063"
     CDS             complement(5378..6397)
                     /locus_tag="IV223_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8710378.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(6419..7426)
                     /locus_tag="IV223_000064"
     CDS             complement(6419..7426)
                     /locus_tag="IV223_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8710379.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(7419..8084)
                     /gene="pgmB"
                     /locus_tag="IV223_000065"
     CDS             complement(7419..8084)
                     /gene="pgmB"
                     /locus_tag="IV223_000065"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8710380.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(8077..10371)
                     /locus_tag="IV223_000066"
     CDS             complement(8077..10371)
                     /locus_tag="IV223_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8710381.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10631..12796
                     /locus_tag="IV223_000067"
     CDS             10631..12796
                     /locus_tag="IV223_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8710382.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            13100..13933
                     /locus_tag="IV223_000068"
     CDS             13100..13933
                     /locus_tag="IV223_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8710383.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(14035..15735)
                     /locus_tag="IV223_000069"
     CDS             complement(14035..15735)
                     /locus_tag="IV223_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710384.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            16242..17402
                     /locus_tag="IV223_000070"
     CDS             16242..17402
                     /locus_tag="IV223_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8710385.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17418..17861
                     /locus_tag="IV223_000071"
     CDS             17418..17861
                     /locus_tag="IV223_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8710386.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(18344..18874)
                     /locus_tag="IV223_000072"
     CDS             complement(18344..18874)
                     /locus_tag="IV223_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8710387.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            18979..19530
                     /locus_tag="IV223_000073"
     CDS             18979..19530
                     /locus_tag="IV223_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8710388.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(19683..20156)
                     /locus_tag="IV223_000074"
     CDS             complement(19683..20156)
                     /locus_tag="IV223_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8710389.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            20294..21766
                     /locus_tag="IV223_000075"
     CDS             20294..21766
                     /locus_tag="IV223_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8710390.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            21763..22473
                     /locus_tag="IV223_000076"
     CDS             21763..22473
                     /locus_tag="IV223_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8710391.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            22825..23109
                     /locus_tag="IV223_000077"
     CDS             22825..23109
                     /locus_tag="IV223_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8710392.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(23390..23809)
                     /locus_tag="IV223_000078"
     CDS             complement(23390..23809)
                     /locus_tag="IV223_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8710393.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(23811..24395)
                     /locus_tag="IV223_000079"
     CDS             complement(23811..24395)
                     /locus_tag="IV223_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8710394.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(24442..25917)
                     /locus_tag="IV223_000080"
     CDS             complement(24442..25917)
                     /locus_tag="IV223_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8710395.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            26098..26409
                     /locus_tag="IV223_000081"
     CDS             26098..26409
                     /locus_tag="IV223_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710396.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(26524..28860)
                     /locus_tag="IV223_000082"
     CDS             complement(26524..28860)
                     /locus_tag="IV223_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8710397.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            29002..29670
                     /locus_tag="IV223_000083"
     CDS             29002..29670
                     /locus_tag="IV223_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8710398.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            29673..30716
                     /locus_tag="IV223_000084"
     CDS             29673..30716
                     /locus_tag="IV223_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8710399.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(30953..31834)
                     /locus_tag="IV223_000085"
     CDS             complement(30953..31834)
                     /locus_tag="IV223_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8710400.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            31993..32721
                     /locus_tag="IV223_000086"
     CDS             31993..32721
                     /locus_tag="IV223_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8710401.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32877..33194
                     /locus_tag="IV223_000087"
     CDS             32877..33194
                     /locus_tag="IV223_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8710402.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            33187..33795
                     /locus_tag="IV223_000088"
     CDS             33187..33795
                     /locus_tag="IV223_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8710403.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            33811..34641
                     /locus_tag="IV223_000089"
     CDS             33811..34641
                     /locus_tag="IV223_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8710404.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            34779..35267
                     /locus_tag="IV223_000090"
     CDS             34779..35267
                     /locus_tag="IV223_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8710405.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            35318..36190
                     /locus_tag="IV223_000091"
     CDS             35318..36190
                     /locus_tag="IV223_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8710406.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            36366..36728
                     /locus_tag="IV223_000092"
     CDS             36366..36728
                     /locus_tag="IV223_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8710407.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            36737..37489
                     /locus_tag="IV223_000093"
     CDS             36737..37489
                     /locus_tag="IV223_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710408.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            37503..39305
                     /locus_tag="IV223_000094"
     CDS             37503..39305
                     /locus_tag="IV223_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710409.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            39582..40622
                     /locus_tag="IV223_000095"
     CDS             39582..40622
                     /locus_tag="IV223_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8710410.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(40674..42818)
                     /locus_tag="IV223_000096"
     CDS             complement(40674..42818)
                     /locus_tag="IV223_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8710411.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(43175..44548)
                     /locus_tag="IV223_000097"
     CDS             complement(43175..44548)
                     /locus_tag="IV223_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8710412.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(44548..44943)
                     /gene="rbsD"
                     /locus_tag="IV223_000098"
     CDS             complement(44548..44943)
                     /gene="rbsD"
                     /locus_tag="IV223_000098"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8710413.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(44971..45888)
                     /gene="rbsK"
                     /locus_tag="IV223_000099"
     CDS             complement(44971..45888)
                     /gene="rbsK"
                     /locus_tag="IV223_000099"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8710414.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(45996..47009)
                     /locus_tag="IV223_000100"
     CDS             complement(45996..47009)
                     /locus_tag="IV223_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8710415.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(47090..47689)
                     /locus_tag="IV223_000101"
     CDS             complement(47090..47689)
                     /locus_tag="IV223_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8710416.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            47875..48576
                     /locus_tag="IV223_000102"
     CDS             47875..48576
                     /locus_tag="IV223_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710417.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            48586..51297
                     /locus_tag="IV223_000103"
     CDS             48586..51297
                     /locus_tag="IV223_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8710418.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            51748..53376
                     /gene="treC"
                     /locus_tag="IV223_000104"
     CDS             51748..53376
                     /gene="treC"
                     /locus_tag="IV223_000104"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8710419.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(53436..54617)
                     /locus_tag="IV223_000105"
     CDS             complement(53436..54617)
                     /locus_tag="IV223_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8710420.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            54869..56392
                     /locus_tag="IV223_000106"
     CDS             54869..56392
                     /locus_tag="IV223_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8710421.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(56461..57189)
                     /locus_tag="IV223_000107"
     CDS             complement(56461..57189)
                     /locus_tag="IV223_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8710422.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(57202..>57990)
                     /locus_tag="IV223_000108"
     CDS             complement(57202..>57990)
                     /locus_tag="IV223_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8710423.1"
                     /translation="RFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLL
                     EQQVRMQQDYIKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPT
                     RQMMNLFPANLSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNL
                     VRMLGIILDNAIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFST
                     KTNPKNEGLGLFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
BASE COUNT        18132 a        10413 c        11856 g        17589 t
ORIGIN      
        1 aacttagttt acagaataga agaacaggtg taatgtaggg ctcgacaaaa tttaaatatt
       61 tttttaggag ggtttcacta tgagtgaatc attgtcgaaa gatctaaacc taaatgccct
      121 attcattggg gacaaagctg agaacggcca aatctataaa gctttgttga atgaattggt
      181 ggatgaacat ttaggctggc gtcaaaacta catgcctcaa gacatgccta ttattacgcc
      241 agaagaaaaa agcagtgcta gttttgaaca cactgtgaat aaaacaaagg atgttctatc
      301 agaaatttca gcgcggatgc gtacccattc cgttccttgg cataatgccg gacgttactg
      361 gggtcatatg aactctgaaa cattgatgcc atctttacta gcgtacaatt ttgctatgct
      421 atggaatggt aataacgttg cctatgaatc ttctcctgca acaagccaaa tggaagaaga
      481 agtaggaatg gaatttgcaa aattaatgag ctataaagat ggctggggcc acatcgttgc
      541 tgatggttct ttagcaaact tagaaggact ttggtatgcc cgcaacatca aatcattgcc
      601 gcttgcaatg aaagaagtga cacctgaact agttgctgga aaaagcgatt gggaattaat
      661 gaacttgtca acagaagaaa tcatgaatct gttagacagc gtcccagaaa aaatcgacga
      721 aatcaaagct cactcagcac gtagcggaaa acatttagaa aaattaggaa aatggttggt
      781 tccacaaact aaacactatt catggctaaa agctgccgac atcatcggta tcggtttaga
      841 ccaagtgatt ccagtgccag ttgaccacaa ctatcgcatg gacatcaacg aacttgaaaa
      901 aatcgttcgt ggtctagcag ctgaaaaaac accaatccta ggtgtagtcg gtgttgtagg
      961 atcaacagaa gaaggagcaa tcgacggtat cgataaaatc gttgcattgc gtcgcgtatt
     1021 agaaaaagac ggtatttact tctacttaca cgtagatgct gcttatggcg gatacggccg
     1081 tgcgattttc ttagacgaag acaacaactt cattccattt gaagatttaa aagatgttca
     1141 ttacaaatac aatgtcttta cagaaaacaa agattatatc ttagaagaag tacacagtgc
     1201 atataaagca atcgaagaag cagaatctgt aacgattgac ccacataaaa tgggctatgt
     1261 tccatattct gccggtggta tcgtcatcaa agatatccgc atgagagacg ttatctctta
     1321 ctttgcgaca tatgtatttg aaaaaggcgc agatatccca gcattacttg gggcttatat
     1381 cttggaaggt tcaaaagcag gggcaacagc agccagcgtt tgggcagcac accatgtctt
     1441 acctttgaat gtcacaggat atggtaaatt aatgggtgca tcaatcgaag gtgcacatcg
     1501 tttctacaac ttcttgaatg acttgtcatt taaagtagga gacaaagaaa tcgaagttca
     1561 tccattaact tatccagatt tcaatatggt agactatgta ttcaaagaaa aaggcaacga
     1621 tgacttagtt gcaatgaata aattgaacca tgacgtatat gattattctt catacgtgaa
     1681 aggaagcatt tacggtaatg agttcttaac ttcacatact gactttgcta tcccagacta
     1741 tggcaacagc ccattacaat ttgtgaacca actaggattc tcagatgaag aatggaaccg
     1801 tgccggaaaa gtaactgtat tgcgtgccag cgtaatgaca ccatacatga acaaagaaga
     1861 acactttgaa gaatatgctg aaaaaatcaa agcagctctt caagaaaaat tggaaaaaat
     1921 ctacgcagac caattattgg caagcgaagc aaaataagca aggtaatcat gttagaaaac
     1981 agcttgaata ctaactgttt ctgacgcatg tttggatctg aagagcaagg ggagacctaa
     2041 gctcttcgat ccctttgttt tgaggttgtc tttgaagaaa cccttcaagc aataaactca
     2101 aagaatcccc tcaaatattc gctgaattgt ttcgtttatt gctgaaacta cttcaaagac
     2161 accgttattt tatccaaaaa atcaaggaga gtgtcaacgg tgagtaaatc tgaagtaagt
     2221 aaaatgggtc ttgggacctt tatcggtctg acaatggcac tgtgtgcaac ggttcggagt
     2281 attcctacgt tagccgcagt cggttggaca ttgatttttt attcaatttt tgcagttttg
     2341 ttttttgccg gtccgatatc aatggtttcc ggtgagcttt ccacgatgct tcctcaagaa
     2401 ggcggtcctc agttatgggt aaaaacagca ctgggcagca aatggggatt tgtcgttgca
     2461 tggctattat gggtgcaaat gttcccaggt atggtaatgg tcgcttctac tttaggacca
     2521 ttgttaggaa acacatttgg taatgtagaa cttggcaata atcactggtt cgttttaggt
     2581 tgtattttag ttatctactg gatcatcacg atcttgaact tgaaattcga tatggcaaaa
     2641 gtcggcggga acatcggtgt atggctaggg gtatatatcc cagtagtcat catgttcgtt
     2701 ttaggtgttt tggcagcctt caaagtcgga ctagtctcaa acggctatct aggtgacttt
     2761 tcatggtcaa aagctttccc tgacttggaa catattgatt cattgaaata cttagctggt
     2821 atcacattta tttttgttgg gatcgaaatg agttctgttt acatgcctcg tttgaaagac
     2881 gcaacaaaaa attatacaaa aggcgtattt atcgctttga tcggattagt tttattgaat
     2941 gtcatcaatg cgatgcttgt agcaaatgtc gtaccagatg gaaaaatgga attagcaaat
     3001 atcacgcagc ctatcttgat tgactgtcaa atcctaggct taccagaagt gatcggaaat
     3061 atcttcagct tcatggtatt tattggtgtg cttttacaat tatcagcttg ggttactgga
     3121 ccatcaaaaa caatcatcca agtagctcgt gaaggattcc ttccaccaaa attcggattc
     3181 cataaagaaa acaaatacgg cgtatctaga aatgtcgttc taacacaatc aatcgttatt
     3241 tcattatttg ctttgcttta tggtgtaatg gatgacgtaa gtgctgtatt cttgacactt
     3301 acaaatgcta caactgttat ttattgtatc gtgtacattt tgattgctgt ttcattgctt
     3361 aaaatgcgta agaaacatcc agaattcgaa cgtccttacc gaattggtaa aaacggaaac
     3421 ggattagctt gggtagtcag ctgcatgctg atcttctcta tcatcgttgt cgtatttgct
     3481 acattaggta cagcgacatt gagtgatgca ttattagtag cagctatcac agtggtcatg
     3541 ttcgttattc cattgatcat caatcacttc aagaaagatt cttgggggat tgaggtagaa
     3601 aaaagtttag aagaaaaata aaaaaataag tctttcagcc gacgttttgt cgctgattta
     3661 gtaggaggtc ctgccaagtg aatagttttt tcatggcagg gccttttcat ttgaaatagg
     3721 agatcgacga tgaataaaaa tatttcttta aaagaaagta tcgctgtatt gatcgtgttg
     3781 ttggcactcc taggagtact gatcatcgga ttccagctct cgccacatat cccgatcctt
     3841 gcagctttca tgtgtttgtt gttttatggc agatttaaaa agttcacatg ggatgagatc
     3901 catgacggga tcatcaaagg aatcactcca gggatcattc caatcctgat ttttcttttg
     3961 attggtgtct tagttgcttc atggatttta tctggaacca tccctacgat tatggtttat
     4021 ggatttaaac ttgtttctgt gcgtttcttt ttaccgacag catttcttgt ttgtgcaatt
     4081 gtcggagtga ctgtcggcag ttcatttaca acgatttcga cgattggaat tgcttttttg
     4141 gggatcggtc atttacttgg attcaatgat gcaatgacta caggcgcagt cgtttccggt
     4201 gcctttttag ggaataactg ctcgccttta tcagatacga ccaacttggc agcgggtatt
     4261 ggaggagtta atctattcga acatatcgct ggtatgcgtt atacggattt cccagcattc
     4321 gtcctttcgt tgatttttta tacgatatta ggacataaca gtcattcagc cgatctagct
     4381 agtatcaatg aaatgatcca aaacatgaaa gcgagctttt ggatctctcc ttggacattg
     4441 attccactag ttattttatt tgggggggca atcaaaaaaa ttccggctat ccctacatta
     4501 ctagcgggat cgacagctgc tctcgttctt tcttttatcc atgagaaaag tttgacggtc
     4561 ggaaaagcag cagacatctt gatgaatggt tatgtcgcaa atacacctga tccaaagcta
     4621 gatgaactgc tttctcgagg cgggatcatg agtatgttag gttctgctag tttgatttta
     4681 cttgcattgg cattaggtgg gttactgatc aaatatctga tcgttgatac catcgttcaa
     4741 gaattaaaag aaaagatgga ccgaccttct cgtttagtcg ggtttactgc cttgagctgt
     4801 atcggaatca atctgatcgt tggagaacag tatctgtcca tcattcttcc aggagaaaca
     4861 ttcaagcgtt cttttgatga cgttggctta tcaaaaacct atttgacacg aacactagcg
     4921 gattcaggtg cgaccgtcaa ttctctcgta ccgtggggag tcagcggcac attcattatg
     4981 ggaacgatga aagttagcgc gttgcattac ttgccattcg cctttttctc gattcttgca
     5041 cctgtcttta caatattagg cggtttttta ttgaatcgtc gacgtgtaaa aaattgaagg
     5101 aaggaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag aaactcaaga
     5161 aaattgcttt ttaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc
     5221 cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc
     5281 ttttttacag ataaacaatg tgttagaagt agtatcttct tttacataaa aagctgacca
     5341 taggataaat ggtcagcgta tttagtgagt cagctattta gtatgggttt tctaaaggaa
     5401 cgactgtctc tcttttgatc aagcgatgag ggatgaccaa acgtacgcca ctgctcaatt
     5461 cttcatggat caattcgcgc agcttttgca tagcgacacg tcctaattcg gagatatcga
     5521 tatcgatact tgtaaggtaa gggtgtgtta atgttgagaa gatcgagttg ttgaagctaa
     5581 tgacggagag tgtctctgga atgacatagc catacatttg cgccaactgc atcgtgcgaa
     5641 gggcgaagat atcgtcaatg accaccaatg ctgtggcttc gttttctttc aacgttttct
     5701 caaaggacag ataatcctcc ggatgctcta gtactacact ggataacgca ggaagaccag
     5761 ccatcatcat tgctttttga taaccgaaat atcgttcgaa atacaaactt tcatgagtcg
     5821 tgtttgtcac aaacaggata ttcttatgtc cattctggat caaaaattct gttgcttgtt
     5881 tacctagaag ctggttatca ttatctacat aaacgatttc gttctcatga tgataaggct
     5941 gtccgatcaa agtgaaaggg atgtggtttt ggtataaata atcaatgaca gtatcttgac
     6001 tatctgaata cactagaata aaaccatcta cttgtttttg taaatgcata cgctggacgt
     6061 tctctaacag caattcaaaa tttttagcag tagcgattgc tgtagtcata tcataattcc
     6121 gcgcttcttc gttgattgct tccatgatct caagataaaa tgggttaccg atccgctctt
     6181 ttgaatcaag agggggtaag attacgccga tggcactgga aatgcgtttt cctagattgc
     6241 gggcagtcat gttaggaaca taacctagtt catccataat ttttcggact ttcttctttg
     6301 ttgtctcgga aatactagga tgatcgttga tgacacgaga gacggtcgag gtggcaacac
     6361 cggctttttt cgccacatct tttacggtaa tagccatgat aaaaacacct ttctcttgtt
     6421 attgtgcaga gaaccgataa atcgtctgac tgatccgttc ttgaccagga tggaggttga
     6481 tcgatccaaa tttggggaaa tgaacgatat cagggatttc ttgcggttcg attgctaatc
     6541 cgtagtttga atgcatctgt tttccattga cagagaaatc agcttcgaat ccagtcgtag
     6601 aaaataatac catactttta ttagaagttg ctatagtcat ttggcgacca gaatgcttct
     6661 cgaataattg taaggaagtt ttggaaagcc taggatgatg caaaagaaat acatcatcta
     6721 gtccatctgg ataacatgca aggatctctt gcagtgttgg ggattttcta aagtcgattg
     6781 gcagtttttc tttttttgtc caatttccag taggcagttt ttcttgatct aattcaagca
     6841 caccatctaa aaagaccgtt aactgatgtt catagatgtc tcttgttcca tttcctgaaa
     6901 ggttgaagta ggcatggttg gtcggattgc agatggtttc aacaaggcta ctagcagagg
     6961 ttgtcatctc taattcgttt tttgttaaac ggtaagtgat cgtagcagtg attggtcctg
     7021 gataggtaga aaattcatct tttaagtaaa agaccacacc tattgatgat ggagttttga
     7081 aaacttctac ttcccaaaat tggaaagacc agcctttcgt gccaccgtgg atatggtgtg
     7141 atccgcagtt tttctccaat ctatgctcac cccaagaagc atcccggatc cttccggcta
     7201 caggtccgac agttgctcca aaaaagcttt tgtctgttag aacatcttcg aaacgatcgt
     7261 aagacaataa aacattttct gattttcctt ctttgtctgg tgcgaagatt tggtgcaggc
     7321 gggcaccgta attgagaaga acgacttcta agtagtcgtt cttcaatgtg attttcttta
     7381 tttcagtctc tggaactggt acgacggaga tctctggatt atccatggtc atgccacttt
     7441 tctttcaagt aatcgatcgt aagaaaagtc gtatcaggga caagcgggat ctcctctcca
     7501 aggtcttcag cacgtcccac gccaattggt tgagcgccac ttgagaggat ggcttggata
     7561 ccggcttgcg cgtcttcgat cccgatacat tcttctgctt ttagtccgac agcttttgct
     7621 gcaaggagga agatatctgg cgcaggtttt ccgtttgttg cttctgccgg gtcggcaata
     7681 gcatcaaagt aaggtgtaag acccattttt tcaagtagga atggcccgtt tttacttgca
     7741 gaagcaagag caatcttgat tttttcttct tttaaagaat ccagtaaagg cacgaccccg
     7801 gggaagacat cttttggttc gatcgtctgg atcatctcta aataataatc gttttttctt
     7861 ttcgctaatt ctgcaaattc ttcctttgaa aaagagtgtt cttttccgcc ataagctaaa
     7921 agcaaagaga gtgagtcttc tcggctgacg ccttttagct gttcgttgaa tgtgtggtcg
     7981 atcgtaatgc cgatttcctg accaagcttt cgccaagcac gataatggaa ttcagcagta
     8041 tcggtaatga caccgtctaa atcaaacaat acccctttaa acatttgctt cctcctttct
     8101 tagcgttatt tctagacgat ccgctaaaga ataagactgt tcatagacgt aaatttctag
     8161 aggagaacct tctaataact cgaaagtaac ttgttcatct gtgactgaga tgaacaataa
     8221 acgtccgcga taattgatat ggaaagaata agctttccaa gtttttggca gaaatggtgc
     8281 gaatctcaac tgatcatggg cagttttcat ttgagcaaat ccttggacaa tcgacaacca
     8341 gctaccagtc atggaagtga tgtgcaagcc gtcttctgtg tcgttattgt aattatccaa
     8401 atccaagcga gctgttcgct ggtacatttc tacagctttg ctttccattc ctaattctgc
     8461 tgctaaaatc gaatggatac ttggcgaaag ggaagactca tggacagtca ttggttcata
     8521 gaaatcaaag tttttttgct tttcttcttt tgtaaaagta tcattgaaga aataaatccc
     8581 ctgtagtaca tctgcctgct tgatgaagca agatctaagg attttgtccc aagaccaatg
     8641 ctgattcaga ggaagatcgc ttgtgggaat atcggataca ggcatgagat ctttatctaa
     8701 gaatgtgtca tgctggacaa agattcctaa ttcttcgtca actgggaagt acatgttctc
     8761 tgcgatatct ttccatttgg ctagttcttc ttcgctgata gtgatagcag cttctttttg
     8821 ataagttaaa taattttctc gcgtgtaacg caagacccaa gctgcgatcg tatttgtata
     8881 ccaattgtta ttgatattgt tctcatattc gtttgggccg gtcacaccgt ggatcatgta
     8941 tttatttgct cgttttgaaa aatggacacg gtctgcccag aagcgtgcga tttctgctaa
     9001 aacttccagt ccttcatttt tcaaatattc cgtatcgccg gtgtaattcg tataattgta
     9061 gattgcatag gcaatcgcgc catttcggtg aatctcctcg aatgtgattt cccactcgtt
     9121 atggcattcg acaccagtga aggtgaccat aggataaagc gcgcctttta atccttgttg
     9181 ctgagcgttg tggattgctt gcggcagttg attatgccga tatttcagca gattcttagt
     9241 tacttcaggt ttggctaaag caagataaag agggacagca tacgcttctg tatcccagta
     9301 agtggctccg ccgtattttt ctcctgtgaa tcctttgggg ccgatattca atcgttcatc
     9361 ttcgccgtag taagtagaga atagttggaa caaattgaat cgaatacctt gttgtgcttc
     9421 ttcgtctcct tcgatgacca catcggcaag cttccatcgt tctgcccaag ccgctgtttg
     9481 ctcttctttt gcttggatat aattgtttgt ctggttcgtt aattctgaga cagctttttc
     9541 tgcttgctgc gcttctggaa tatctctact agtaagaacg ataatttctt tttccactga
     9601 ccatgtctca ccttgagata attctgcaga caagcaacca tgtaatgcta aagtatcttc
     9661 gtgatagtca gggatgactg attttccatt aacgaaatgg cgcattgctg cagtaacagt
     9721 aaattgttca atcccaaagt tgtttggaat agttttagtc gtcaaatagc cgatttcttc
     9781 tatagttcca cgatcaatct cattccaaaa atgttcatca tagttgctgt cttcgttttg
     9841 aacatttcca tccaattttg atacgagttt gactgttccg ctaccttcaa gcatttctat
     9901 agttaaatag atataagctg attctttttt gacaatgctc agaaaacgtt cgaaggaaat
     9961 gcgtacttta ttagtagaag tagtgattgt gaatgtacga gataatacac catttttcat
    10021 atctaattcc cagtaaaagt cttctggttc caaagaagcc aagtcgatca gctgatcgtt
    10081 gatcgaaata tctatcgcga taaagttcaa ggcgttgatc acttttccaa agtattctgg
    10141 ataaccattt ttccaccagc cgacacgtgt cttatcggga taccagacac ccgccaaata
    10201 agtaccttga tggtggtccc ctgagtaagt ttcttcaaag ttcccgcgca tccccatata
    10261 accgtttcca atacttgtta aagattcttg cagtcgaaga ttttttttat ctaattgtgt
    10321 cgtgctgatt ttccatggat caatagcaaa tagtcgtttg atctgtttca tttttctcct
    10381 cctaatgagc tgttttatac gttcataata aacgcaaccg attgcacaag tcaatagaca
    10441 aaagaaaata tttcatagaa aaaagcttaa aaaaataaat ttttaggaga aaattataga
    10501 agggaacaca cgtattatag gttttataga atggtataga cagaaaaata aaaaataaag
    10561 catttacaaa aaagaaaacg cacgctataa tacgtttaac gaaaccgatt gcgtaaagga
    10621 gctaaacgga atgaaaaaga tatttagttt tgagttttgg caaaaatttg gaaaggcttt
    10681 aatggttgtg gtagcagtta tgccggcagc tgggttgatg atcagtatag ggaaatcaat
    10741 tcccttgatc aatcctgatt ggacgccgct tgtcacaacg ggcggagtaa ttgaaaatat
    10801 tggttgggca attattggta atcttcatat tttatttgct ttagcaatag gaggaagttg
    10861 ggcaaaagaa agagcaggcg gagcatttgc agctgggatc tcatttattt taatcaatcg
    10921 gatcactggg gctatctttg gtgtcacaag tgacatgctt gcgaatgaag atgcatttac
    10981 ccacactctg ttcggtacga agatcatggt taaaggattt tttacaagtg tattggaagc
    11041 accagctttg aatatgggag tattcgtagg gatcatcgct ggatttgtcg gggcaatggc
    11101 ttataataag tactataatt atagaaaatt gccggatgct ttgtcatttt tcaatggaaa
    11161 gcgctttgtt ccgtttgttg ttatcttatg gtcaacaatc gtggcattga ttcttgctgt
    11221 ggtgtggcca aacgtacagg ctggaatcaa taattttggt ttatggattg cagaatcaca
    11281 agaaagcgca ccgattctag caccgttttt atttggaaca ttagaacgtt tactattgcc
    11341 atttggattg catcatatgc tgacgattcc tatcaactat acacagcttg gcggatcgta
    11401 tgagatttta tcaggcgcac aggcggggac acaagtattt gggcaagatc cgttatggtt
    11461 ggcttgggcc actgatttag tgaatcttaa aggtgcaggg gatatgtcgc aatatgagtt
    11521 tgtgttgcat aactggacgc cggctcgttt caaagtagga caaatgattg gttcttcagg
    11581 tatcttgatg ggaatggctt ttgcgatgta tcgtaatgtc gatcctgata agaaagcaag
    11641 atataaatca atgtattttt cagcagcgtt ggctgtcttt ctaacaggtg taacagaacc
    11701 attggagttc atgtttatgt ttgctgctgt gccattatat gtcatttatt cagtgatcca
    11761 aggggctgca tttgcgatgg cagatatctt gccattgcgg gtccattcat ttgggaatat
    11821 cgaattgctg actcggacac cgttagctat caaagcgggt ctaggtggag atcttatcaa
    11881 cttcgtgttg tgtgtgatcg cttttggtgt tgttacgtat ttcttagcaa atttcttgat
    11941 caaaaaattc aactttgcta caccgggacg taacggaaat tacgacaatg acagtgaaga
    12001 aaccgttgta tctaacagcg gaacaggaac agcagatcag caagtggtcc agatcattca
    12061 tttgctggga ggaaaagaaa atatcaaaga tgtcgatgct tgtatgactc ggttacgcgt
    12121 aagtgtaaat gatcgagaaa aagtagggac tgaagaagcc tggaaacgag caggggcgat
    12181 gggattgatc gtcaaagaca atggagtcca agcagtttat ggaccaaaag cagatgtctt
    12241 gaaatcagat atcgaagatc tgctgcaatc aggagcagag attccagagc cagaaatggt
    12301 ttctacagga aatggacaag caggtagtaa gcaatactta ggtatagaac aagaactagt
    12361 ttccgcagct tctggagaag tgattccttt atctgaagta aaagatccgg tattctcaca
    12421 aaaaatgatg ggtgacggct ttgctgttat tccaacatca agagaagttg ttgcgccgat
    12481 cgctggaaaa gtgacaagta ttttccctag caaacatgca attgggctgg aaacgaaaga
    12541 tggtatcgaa gttttgatcc atatgggaat cgatacagtc caaatgaatc agccagcttt
    12601 tgagattgct gtcaaagaag gccaagaagt aggaacagga acgaaattag ctgagatgga
    12661 tcttgatgtg atccaaaacg aagaaaaaga tccaacgatc atgatcgtat ttacaaacga
    12721 taaagtagaa gaagtagtga tcaagcaatt aggtacagtg acagctggta aagtgatcgg
    12781 ctccattaaa atgtaaggag aggttgtgaa aaagctgtcc tgcatcaagt cataagaaca
    12841 gccaaacaaa aatagctcct cctatttttc gtttggttgg gcttattacc gagaagcagc
    12901 agtttgaata ctgtttagtg gagaggtcgt gaaaaagctg ccctgcacca agtgataaaa
    12961 acagccaaac aaaaaatagc tcctggtcca ttgagcttat tactgaggag ttagcaggag
    13021 aacactattt gctggagagg ttgtgacaaa aattttgtca caaccctttt gtttcgaaat
    13081 gaagacaagg agaaaaccaa tgaaattcat gacactcaac acgcatagct ggcttgagcc
    13141 agaaccagag aaaaaattgc aagaattagc agataagatc ctgttagaag attatgaagt
    13201 gattgcttta caagaaatca accaattact ggaatctgaa gaagtagaac ctgcgaagct
    13261 aatgaagttc tgtccggtaa agaatcaagt acctatccgt aaagataatt ttgcctaccg
    13321 tttagttcag cttttaaagg aacacggaaa agagtatttt tggagttggg aaatgagcca
    13381 catcggttat gataaatatg aagaaggaaa tgcactgctt acgaaaaaaa cattggaaag
    13441 tcaggtcctt accgtctccc aatttcaaaa gaaagaagat tatcagacac gcaagatctt
    13501 gattgggaaa acaaagatag atgatcagga catttttgtc tcgagctgcc acttttcttg
    13561 gtggacagat aaaaaaagtg gcttttattt tgagtggaaa aacttggaaa actacttttt
    13621 ggaaactcgc gtaccacttt ttttcctagg tgatttcaat aatccagtag actctcaagg
    13681 ttattatact gtaagagaaa gttgccttct tcttcaagat tcgtatgttg tcgcgaatga
    13741 aaaaggaaaa gcagctactg tggaaaagaa aatagatggt tgggaacaaa atacagaaaa
    13801 attgcgtata gattttattt tcgtccctga aggaatgcag gttaaaaaat atcaacggat
    13861 ttttgatggg atcgatagtc caatcatcag tgatcactac ggggtggaaa tcgaattaga
    13921 tgtgaacgaa taaaaaatat aacatataac ataaaaaaac tgattaaaat aaaactctcc
    13981 aaacgattat accgtttgga gagttttttg atcggatgta tttatccctt ttgcctaagc
    14041 acgtaaaagc ttgtatctat gataaataaa taaacatagc aaggtaaacc agccaatcaa
    14101 agtaagagaa agaatgaaat agaaatagat tggcgtttga taatgaagcg tgagctcgtt
    14161 gattcctttt tgctgcttca cgattggggt cccgatatct gttacttcat aatcagtcaa
    14221 tgtttttcca ttttgctgta gcacagtccg atcgtagacg ataacaggga tatgaacagt
    14281 tttgtcccca gtgtttttcc acttgattgt caaaagaccg cctggatgtg tttttataaa
    14341 agttgtttcg ttttctagta tttcttcgtt ataccggtcg tatttattgt cttttgtctc
    14401 cttatagatt ggcagatagt caggtgtggt tttttggaaa acgaacaagc tggtagacat
    14461 atctttgctg aagaatgttt tccttgcttc ttccggcgta tcgaatagat aagtatgtcg
    14521 actgacaaat gtttcatttt cccacttttc cagatgctgg tataagttct ggatcgtctg
    14581 tgctgaaaaa ataagaatgc caaatatact taaaagacgg aaccactttt gttcagacca
    14641 ctggttcagt gaaagggcaa ggtaaagtag aagcaatagc gtaaatggca cgaaaaatcg
    14701 aaatggaaac tggatcaaac taataaaagg cacatttttt tcgatcaatg tcgtccatgg
    14761 aatcaagcta gtagaaagaa aaaagaaaac aagacttgtg tacaacacga ttttttggaa
    14821 taaggtgttc ttttgttgaa agaaaataaa aagcaaaaaa cctgataata tcaatatagg
    14881 aaaggcctta gggtaatgga gccaataact gccatccaga tctatcgcct tttgactcat
    14941 ttctctgtta acaaagggag gaagaatctt attcccactg taaacaatag ctaatcctgc
    15001 ccaaatattt gctgtcagaa caaaataaag caagaccgac ttaatcagtt gaagcagtgc
    15061 ttttcctttt tcagtctggt taaaaaagta ataaagataa aaagggatat acatcaaagc
    15121 taacatgatc gaagacagta aatggatctg tgtcataagt cccactgaga cagcgacttt
    15181 gataacaggg aactttttat gaaggacgaa atcagcaata gggatcatac ataaaggata
    15241 aaaacaagtc ccccagctag tgaatccttg gttgatagac cagtattgaa tagaatacgt
    15301 actcatgaat aaaacggcta ctggtacact tacccaattt cgaacattga catatctcag
    15361 taaaatcagc atagaaaatc ctgaaataag ataaagaatg aaatttgaaa gaatctgata
    15421 atgaaaccag ctacctgcaa gcaacacgat caatccttgt aaataagcga aaaatgggcc
    15481 gtaaacggca ttcacgatcc tgccactttg ctggaatcca taaagggaag gaaaataatg
    15541 gaaatcccca tatttaattt gttgcgcggc atcatagaac cgattgtaat ggaatggggc
    15601 atcagcaccc aaaattagtt tatgtgtgaa taaatgaggc atgagaaatg ccaaagcaat
    15661 gaaaaaaatt gctaaaaatg ggccatattt tttcaattgt cgaactcctt tgcaaacttt
    15721 agataaattc atcatatatc ctatgcgaga ggtaggcaag aaattagaaa attcatgaaa
    15781 atatttatga attttacaag aaaaacataa gtgaaagttc taaggagaga ggatgcgaca
    15841 taagttttgt tgcatctcct tgttttgaaa cagaattttt cttaatactc gaaacaaagt
    15901 agctttttca tgacctctcg gtaaacggtg ttcaatcagc tgcttctcgg taataagctc
    15961 actagcctag aaaatatgag aagctatttt ctgactagcg ctcttattac tcaaagcata
    16021 acgcagtttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    16081 gcccaaccca acaaaaaata tgagtagcta tttttttggg ctgtacttat tacttgatgc
    16141 aggacagctt tttcacaacc tcatcggctt tcattttcta tttttttcct gaacccatat
    16201 aataaaagaa agaacaaaaa aacttgcaag ggaggacaca tgtgaagcag agtaagcaaa
    16261 aacagtctcg tcgacttttg tttcgtttgt ttttattaat atgtatcgtc gtagggttac
    16321 tgatcgcttt atatccattt tatgtggatt ctttaaatag ttttattgat caaaaacgta
    16381 tcgaacaagt acaaaaacgg accgcagctg aaattgaagt gcagcgaaag aaaatggagg
    16441 agaaaaacca acgattaact gaccaaggat tcaatcctgg tactgatcct ttcaatgaaa
    16501 agaacagaaa tgaaagtaca acatccagcc aattagagga atggctgatc ggaagtatca
    16561 atatacctaa gatccaatta aatatttcac tttatgatcg gctcaatggt aggatccttg
    16621 aaaatggagc aggcgtcttg caaggaacct ctttcccgct tggtggaaat tcgacgcatt
    16681 cagtgatctc tgctcacagc ggtttaccta atcgacggtt atttaccgaa ttagatcgtt
    16741 tagaaaacgg agacacgttt attttgactg ttttggggga gaaattggct tatcaagtta
    16801 aaaatataca agtggtactg ccagatgaca cgtctgttct aacgatttca gaaggagaag
    16861 acttagttac cttgcttaca tgtacgccgt atatgatcaa cacgcatcgt ttgctagtga
    16921 caggacatcg gattccttat aacgaatcgg taaaaaaaga agaaggaaaa ggaaatcagg
    16981 aaagaaccat gcggcaatgg tttattcttg caggaacgat aatagctgta gtcatactgt
    17041 tagtgtttat cggacgacta atctatcagt atagattaag taaaaaagtg cttgattttt
    17101 catttattat tagtgattcg tcagggaaac cggtaaatgg tagttcattc attttgaaac
    17161 ataagaaaaa aacacttact agaaatggtg tgccgttttc agtacaaagc gaccatcatg
    17221 ggaaagtcaa gctggatcaa ttgcctggtg gaacttatcg aatcgtttca gctgatgatc
    17281 ctaaagttgc tgtttctttt ggtattagaa aagtaaaaca agagaagata tacttttttg
    17341 aggggcgaaa acttgtaaaa gagcttcaaa aaaatggttt ttattttaaa atcaatgaat
    17401 aagtaaggag agaaaaaatg atccgcacaa caacaaaatt gtctgccaaa gaactggaat
    17461 ctgttctatc gatttggctg catgctaact tggattctca tgaattcatt ccatcaactt
    17521 attgggagaa tcaagtagaa tctgttagga aagctttacc agatgcagtg atttacttag
    17581 cagaaataga tcaagaaatt attggttttg ctggggtgat tgatcagttt atcgcaggaa
    17641 tctttgtgaa aagggaatac cggcaacaaa gaatcggtac gcaactaatg aaaaaaataa
    17701 aagaaaccaa taaaacactg actttgacag tctatcaaaa aaatcaatca gctattcatt
    17761 tctatctttc acaaggtttt acaattcaaa cacagcaaaa agatgaagca gtaaatgagg
    17821 aggaacttgt gatgaaatgg aatcaaaacg ctgatttttg atgaacagaa taacgaaata
    17881 atgctcccag cctaattttt tgctgggagc attatttcat tttgaagtca ctaatttaac
    17941 tgtgtgaata tgcagttgtt acaatctttg ataaacggtg ttcaatcagc tgaccctcgg
    18001 tattagctca aaaaacggca aaatatgagg aactattttt ccatttgttt ctctaatact
    18061 caggtcaaaa cgctgatttc acaacctctt gataaacggt gttcaaacgg ctgcttctcg
    18121 gtaataagcc cactagccta gaaaatatga aaagctattt tctaactagc gttcttatta
    18181 ctcaaagcat aacgctgatt tcacaacctc ttgataaacg gtgttcaatc agctgaccct
    18241 cggtattagc tcaaaaaacg gcaaaacatg aggaactatt tttccgtttc tttctctaat
    18301 actcaggtca gaacgctgat ttcacaacct ctctttcaca accttatttt actcgtttat
    18361 aattttcatc gaagtatttt ccgctacgga tatcatctgg cattccatcg tatggttctt
    18421 cgctaataac atcttcattg tctatacctg catcacccat ataagtgatc gtagcaaatt
    18481 caggaacaag taattttgga taagtttcat atttttcacg agctgcttcc atttctgcga
    18541 tatgttcgtt ttggaaagta acagtgattt ctggatgttc ttcaacccat ttagggaaaa
    18601 agatcattcc gtgaagtttc ttttcatttg gcataaagtc caatgccacg tctgtccctg
    18661 ttggctctgt ccaagtgatt ttgaatacgc cgtccgtcaa tttaacgata tcagcttctt
    18721 ggtcttttac ccaacggcct ttgaccattc cgccatggat acggtaatca acagtatggt
    18781 catttttggc ataccattca tattcccaac cattgtcata cgtatagata aagtgagttc
    18841 caataaaatc ttccaattct ttaaattctt tcatgtttaa gcatctcctt ataaattaaa
    18901 acatgtaata aactacatgt tttcgattac tcatttatga tagaatggat ttaaagattt
    18961 gtcaaggagg gaaaactgat ggcaagaaaa aatacacttc gatatatttt gttagggtta
    19021 ttaagtaaga agaagatgac aggatatgaa ttgaatcaat cgttcaaaaa tgaaatagga
    19081 gaattttggc aagcaaaaca tagtcagatt tatcctgaac ttgcaaaaat ggaagagcaa
    19141 ggaatcattc agcatcaagt agagatcaca ggtgaaaaat tggaaaagaa agtttatgaa
    19201 ttaacagaag aagggaagga acaattggca gagtggattc atacacccac caatgaacta
    19261 ccggttaatc gagacgaatt tgttctgaaa ttgtatttcg tcaaagacgt gaatgatccg
    19321 gcattagtag aaattattca gcagcagcgt gaccttcatg aagaaaaacg acttcatcta
    19381 ttagagcgaa aaaaaacgat ctttcctact gaaaaagaaa aacatgaaaa ttacggacac
    19441 tacctgattt tagcgcatgc gatcaatcgg gaaacagaat atgcaaattg gctaacagat
    19501 gtattagaag aaagtaaccg aaaaaaataa aaaaatatcc gaacagtgat ggagaattcc
    19561 tttctatatg aaagaaacgc agatcaccgt tcggatattc ttatgttcaa acaggccttt
    19621 tttcaagtat ttatgtagca tctacttatt aaaaagcttg gtacttagga gattagtggc
    19681 gtttagagtt cttttaacaa ctgttcgtag tcagcttttt catcgacagg aaggagcttg
    19741 taagttaatt cgtctgtctc gttgtcatga atgatgtaca tcttagcatt ctcttctttg
    19801 aagttaccga tttgtaaaat cgtatagcgt tcgtcgatca attcgatatc tttgattgct
    19861 tttgaaaaaa ctttacggac atggtgcatt ttcttttggc ggatttctgc cttttctgca
    19921 gcttttgaca aagtaaatcc gtcatctaaa tattttttga tcagttctac ttttatgact
    19981 gtaggtaaac gatattttct aggacaattc gcatcagctt cgatcgaacg aatgaatccc
    20041 ttttgttccc aataccttaa ttgacgtgga gacacaccta cgatatcact taattcactg
    20101 atgccaacaa ccaattgatc attttccaac aaacgtttaa gggaagcccc aaccattttt
    20161 gtcactcctt ttgtcctctt gcttccctta attatatccg tattaactta tgtgtcaagt
    20221 aggttgacaa atagcgaatt taggactaaa gtattctagt agtttcagaa aaaattcaga
    20281 aagaaggaga aatatgagta acaaccaacc cgtagatatt tacggaaaac catataatcg
    20341 gacactttta gttgttgtct tattaattgg tacattttgt accgttttga atcagacctt
    20401 attgacgaca gcttttccta cattgatgaa agctttcgat atttcagcgt ctgatgtgca
    20461 atggttaacg actggtttct tgctagtcaa cgggatcatg atcccaataa cagcatggct
    20521 gatcaataaa ttcagttcaa gaaatttata tctttctgcc atgacgatct ttttgatcgg
    20581 aacgattact tgctttacag cacctaattt tagtacatta ctaatcggtc gtttgatcca
    20641 agcttgcggt gtgggagtat cgatgccatt attgcaaaat atcatgctat ctattttccc
    20701 gccagaaaaa agaggatcag caatggggat gtcagggatc gttattggac ttgctcctgc
    20761 tttaggccct acattatctg gttatatcat tgataactac cattggcgtg acttgtttgg
    20821 tatggtactg ccaatcgttg ttcttgtctt ggttttagca ttcttcctaa tgaaaagtgt
    20881 tattccattg tcaaatccta gcattgatat tttatccgct attttatcaa cgattggatt
    20941 cggtagttta ctttacggat tttctagtgt aggagatgac ggctggggaa gcttgaaagt
    21001 aattggtttc ttagttgttg gtgtgatttt catcgccttg ttctcatggc gtcaattaca
    21061 tttggaacat ccattcttgg aacttcgtgt attcaaatca tctacattca cgattgcagc
    21121 tgttttagct ggtgtaacaa atatggcgat ggtaggggca gaaatggtcg taccattgta
    21181 tattcaaaat attcgtggag aatcagcatt ccattctggc ttgatgttgt tgccgggtgc
    21241 attgatcatg ggggctatga tgcctgtgac gggtgcgatt ttcgataaat acggcgcgaa
    21301 acgtttagcc atcacaggga tgttcatttt gacagcggca actgcaccat ttgctttctt
    21361 gacaaaaacg actccagttt tatatatcgt gatcctttat gcgatccgta tgtttgggat
    21421 ctccatggtc atgatgccgg taacgacttc tggtatgaat gcattgccaa ataacttgat
    21481 cagtcatggg acagcggtaa ataatacatt ccgtcaaatc gcaagctcga ttggtacagc
    21541 tattttgatc agtgtgttga cgaatgtgac aaaagcaaat atcccaagca aatctgtatt
    21601 ggataatacg ccattagctt ataaagatca ggcaatcaat gccacactgt caggttacca
    21661 tgccgcgttt atcgtagcga tcatctttgg gattcttggc tttattattg cctttttatt
    21721 gaagaaaaaa gaacctgttc taatgaaagc gggggataat gcatgattat tggagttttg
    21781 attgtcagct tgcttgcttt tgctttaacg aacgtgttcg ctaaaaaaac atggcaaacc
    21841 tttttatctt tgattttcgg actaatcttc gtcgcttcac taagtttgat cgtcgctaat
    21901 ttgtccaatc actttggaat ggaaaaagta acggaaacaa aaacagaaaa aattgtttcc
    21961 agtgctgatt cccaaggagc agatatgctt ttgtataaag ctttagggaa tggaaaagaa
    22021 aaagtgtatc tttaccgaac aaatgaaaaa caagaaaaac caaaagcaac tggtacggat
    22081 aacgaaacaa ataaagtaga aaagacagac ggcgatgctg aaaaagtgac gaaaactact
    22141 tattgggaat acaaaaacga catgtataaa ttctggttca atattgctga taacaatcac
    22201 gaatatgaca gccgagtgaa tacattcaaa atcccagaga catgggtaga attaagtacg
    22261 gaccaagcag ccaagttagc tgaattagtg aagaaacagc aaagtacgat ggaaagtgaa
    22321 gcaaaagctt atgttcaaga cggtatggta aaagcaatga ctgaaaatcc aaaaatgagc
    22381 aaagcagagc aagaacaacg tacgaaagcc ttagctgctg aattccaaca acaagcattc
    22441 gcaaaattag tgaaagaagc aaaaggcgaa taatattttg aacaccatgt aggaggttgt
    22501 gaaaaagctg ctctgcatca agcagtaaga acaatcagat aaaaatagca ctccatattt
    22561 ttcatctgat tgggcttagt gccgtagatg caagcttttg aacaccgttt aaggaagagg
    22621 ttgtgagcct gccgtttaac tctgagtcac aaggagcaat ttcaaaaaac agcttctcat
    22681 gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcgaa
    22741 aaaaggaact gcgacaagac ttttgtcaca gttccttctt ttttttgcta tgctatacta
    22801 gatgtaaaaa ataggagaaa taaaatgagg gaaatttatt cttttgaatc aaaaatctat
    22861 gcttccgaag tcggcaaggg cggggcatat attatttttc cgtatgatat aagaaaagaa
    22921 ttcggtgctg gaagagttaa agtccaagca acttttgaca atgtgccgta tgaaggcagt
    22981 atcgtgaata tgggcgtgaa aaatccagat ggttcagtct gctacatcct tggactacga
    23041 aaagatattc gtaaaaacat cggaaaagaa atcggtgata ttgtagcagt tacagtgaag
    23101 caaaaataaa agaggttgtg acaaaagacg tgtcacaacc tcttgataaa cggtgttcaa
    23161 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaaccc atgtttacaa
    23221 aatttttctt actactcgaa acgaagcggc tttatcacaa cctcttgata aacggcgttc
    23281 aatcagctgt ccctcggtat tagctcaaaa acggcaaaat atgagaagct atttttccat
    23341 ttttttctct catactcagg tcataacgct gatttcacaa cctcttcttt cataatggtt
    23401 tttcaccatc tttataataa actggttctt tgatatggaa atctattttt tccaaccaat
    23461 ctaaaatctt gtaacctaac tcttctgaca tcggaaaaga ggcgttgatt tgtgcattgg
    23521 agatatggtc aacatggata ccggaagtga tcgtacaact tcccggaacg gcagttttga
    23581 tgatcacagc tattttttcg gctaagacat cgtctttatg gaaccgacca tcgtggctag
    23641 ggaaacgtac agtttgcatc tctgtatcac gagaaaatgt agtgactgtt ccgatatgtg
    23701 gtgtatctcc gcctgttaaa gtgatcaata agtctttacc gattacttta acagcagctt
    23761 ggatcgtgaa acctagtttg gttacttcaa ctatcgtttc tttttccata ttaatcaccc
    23821 tgccaacgtt ttgaatgctc atttacaatt tcaaattgat ccaaaagttt cattgtatgg
    23881 tgattaacaa gatcttggat cgtttgaggc tgattataaa aagcaggaat tggtggtatg
    23941 atttgcacac ctagtcgtgc taatttagtc aagttttcca agtgaatggc atttaatggc
    24001 gtttcacgag gaacaatgac taactttcgt tgctccttca ttgttacatc agcagcacgt
    24061 gcaatcaggt tgtcgcctaa accaacagag atacctgcga ctgttttcat acttgccggt
    24121 acgatgatca ttccatcggt caaaaatgat ccgctggcga cagcagcacc taaatctttg
    24181 tttgaataat ggtaatcaaa caggctttcg aattcagtta gtgataagtc ggattccagc
    24241 tttaagttct gttttgccca tggactgatg attccgtgtg tttctatcat cggatattct
    24301 tttagtttct tcactaggtt gatggcataa atggtgcctg aagcgccact tacaccaatc
    24361 acgatttttt tcttccgttg tgccaattgt tccattttcc gcactccttt ttatgatgtc
    24421 aaaacagttg cagatcattc attatttcaa atatttttcc caatcttcta cttcttggaa
    24481 ttttgcacgt acaaaatgtt ctttcatatc gaaaggaact gtaccgtcaa agatagtttt
    24541 tgcacttatc ccacggaaac gaatagagcc aggattatat tctggtaatt ctgatgggtc
    24601 caatggatgg ttcctcattc ctgggatcac gatgatatct ttatctcctt ggaaacgagt
    24661 gttgaaagtc caaactacat cgttcatgtc gaaaatatca acatcttcat cgactaaaat
    24721 gactgtcttc aattctttga aggctgataa agcaagaata gctgcttgac gctgaatacc
    24781 ttcatccgct tcgttttctt tacggatttg catgatggtc atcaatttac caccacctgc
    24841 aggaggattg tagacattta ctacttttcc tggaatagct ttatcgacca aggcaagaat
    24901 acttgcttct gtaggaatac cggccataga tacgtgttct tcactgggac caatcgttgt
    24961 ttgcatgatt ggattgtctt ttcggtgagt aactgctttg actttcacta cttgtacagc
    25021 tggattagca tccccagtat agccaggaaa ttctggcatt gcttttcctg agttcgtgtt
    25081 gatatcttct tgcattgttt catttggcat gatttctgct tcaatgatga attcagcacg
    25141 agcaataccc acttcatcga ctgctatacc atcaacgatt tgaataggtt cgttacgcaa
    25201 agctcctgca caccatagtt cattgtagcc aagtggagtg gtaggtggtt cgaaagtcgt
    25261 gccaatggca attgctggat ctaaaccaat attgatagtg ataggcattg gtttattcaa
    25321 ggcttcgaat tgtttttgga agtgaccaat gtggcgtccg ccaggcatga tgtacatacc
    25381 gatcgtatct ttatcttcta gaaccatacg atggatcgtt acatcgctca ttgttttttc
    25441 tggatctgat cctaagacta cacccattgt gatgtatgga ccagcatctt caggagtatt
    25501 tgtaggtgca ggcaaaatgt tgcgaatatc gaagtctgca tcggtagcta gatgaacgac
    25561 ttcttgtgct ggagcgtctg ctttgtctac tttcacaggt ttgattggat tttctacagc
    25621 gtctttcaag aaacgaccaa gtgttcgata atcatggtgc aaaatttcac ctacacgttt
    25681 acgactagcc attgttccga tgttcacacg tgtgccgggg aaacctttga tattgttgaa
    25741 agtcatagct ggtccttctt gagtcggacg ttttactgta ccgccagcac cgatataacg
    25801 gtaaacacca gataattctg cttcaggatc gatttcaata tctgtttcgt gatattgacc
    25861 tggaagtgtt tttagttctt cgatgacttt tcttaaatca tacggttgat ctgtcatgag
    25921 agattcctcc ttagttgtct ttacctatct atagtaaaag gtgaaaaaga ggaaaacaat
    25981 tcaacttgtt cggtaaagta ttctacaatg gaatggatgc gtttttttga taatagaata
    26041 aactatgata tacttatcac taaaagaaag cgcatactta gtgttggagg aaggaaagtg
    26101 aaaaaaatgg aggagtcatt tcctaaagca gtaaaagtgg aaaatatagc gaatattttg
    26161 aaagttacat ttgaaaacgg agaagtaaaa tacgtgaaat ctcattggac agaagagata
    26221 accgatgcct tgcaatttgg aaaaaaagga agaggaaaaa gaaaaaatct tctcgcactt
    26281 tctcgtaata tgtggattgg gactgaagta acgattgaag cagatggaac agtattcata
    26341 aatggaaaag acaggtatac gccagaagag ttgtggtaca aaggaaagaa aagtatacca
    26401 gaattataaa aaataattaa aaaaaacagc gcgaaacaag gaacatatta cttcctcgtt
    26461 tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt ggttgtcaca
    26521 accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc gtgcaggaag
    26581 atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg catggacagg
    26641 atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat tgatagggaa
    26701 cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta aaatacacgt
    26761 ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt ttagtacacc
    26821 gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca tgatcatact
    26881 tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat ccatcaatcc
    26941 gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat cttcatctga
    27001 tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt caaaattgtt
    27061 cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa gcaatgtttg
    27121 tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt ctctttgagc
    27181 tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt gatcgctata
    27241 agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta ggatccccga
    27301 gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat tagggataaa
    27361 attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga atgttgtacc
    27421 aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa gattcttcaa
    27481 tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg ttgctgaggc
    27541 tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt tgctagtgtc
    27601 aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg tactagataa
    27661 agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta ctttgccatt
    27721 tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat tacttaagtt
    27781 aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg tgtgctgtgc
    27841 tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat ccgctttgct
    27901 tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa tagtaatcgg
    27961 aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc cagaagaaag
    28021 ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg tcaagaaagt
    28081 acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc aaaaactcat
    28141 ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag gatgggaatc
    28201 tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat gggccagttc
    28261 aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga tcaattccat
    28321 gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca aaaggatatc
    28381 tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac cagataactg
    28441 atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat cttctgcccg
    28501 ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa cgttttcaat
    28561 aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag agttgtttcg
    28621 gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga ttaccatatc
    28681 accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt taccagaacc
    28741 actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca caccatccaa
    28801 tgctttggtg gtggtttcgc cgactttata atattttttt atatctttta attgaagcat
    28861 aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca gaacaatgat
    28921 cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc tagaaatttt
    28981 tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga tccgcaatta
    29041 cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac cgcattgaat
    29101 ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat tacagatatc
    29161 atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac cagatttgac
    29221 acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg cgggttcaaa
    29281 ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat cctacgtgtg
    29341 gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat cggacaaaca
    29401 aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat tctgcctcaa
    29461 aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt cactcgccaa
    29521 cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac gatcgatgta
    29581 catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat catcacagta
    29641 cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt tcacaattat
    29701 ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg tgttttctcg
    29761 gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct ggaaaaccaa
    29821 cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca ggaatatcga
    29881 tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat atgagtctgg
    29941 tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca gaagtacagc
    30001 aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag acactgagga
    30061 atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca atccaaggat
    30121 atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata tttttatatc
    30181 ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta aaattgaaca
    30241 aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat gaacaaatca
    30301 gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa ttggatatcg
    30361 aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg tggctgaata
    30421 tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc aaactatttg
    30481 agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg aatgaagaga
    30541 ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa atttctggga
    30601 atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga cgtattgatt
    30661 atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa caataggaaa
    30721 aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt cgtttttttg
    30781 tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt cttattgaac
    30841 tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag agagcgaaag
    30901 tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg gcttagtttt
    30961 cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca ccgactttac
    31021 gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca gtattttctt
    31081 ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca tttgcaatca
    31141 tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca aatccagctt
    31201 cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg agcaaaaagt
    31261 tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga tgatttttag
    31321 gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa cttggtgggg
    31381 cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca ttgtaagtta
    31441 attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt ttcaaaaact
    31501 cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag atatttaatg
    31561 aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg tattgttcta
    31621 cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg atttttctat
    31681 gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa
    31741 tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt gtttcggcta
    31801 aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt attccaataa
    31861 agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta caatctttct
    31921 tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt agaaaaagag
    31981 aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt gtttggcgga
    32041 ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca gctgacgatg
    32101 gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat gaaaaatatg
    32161 ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt tcatttaggt
    32221 aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat cttccctagt
    32281 gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt cattgttcta
    32341 ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac aggagacagt
    32401 acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt tgcgtgtaat
    32461 ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta tattttgttc
    32521 ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga ttttaaagct
    32581 tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa aatgttccca
    32641 gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat gtggacaaat
    32701 tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct gactaaaaag
    32761 gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa ctatattata
    32821 tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt gacgtaatgc
    32881 acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta agcaaggaag
    32941 atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc agtgaatcta
    33001 cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc tatgatgaac
    33061 cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt cgtcatttag
    33121 aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta ttggaggaga
    33181 gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac
    33241 gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg aaaaacatta
    33301 gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt aaaaatcagt
    33361 tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa aagacaaatt
    33421 cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat tccactagca
    33481 ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct catttttagt
    33541 atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat ctcaggaatc
    33601 agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc
    33661 acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg gcttttttat
    33721 gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag agatctgcat
    33781 tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt gattaccggt
    33841 attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt aggcgctcaa
    33901 aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga gacaaaaaaa
    33961 atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc
    34021 ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga aataaaggag
    34081 aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat cattagttta
    34141 gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt atctgaattg
    34201 acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat
    34261 actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac aataactgcg
    34321 aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt gatcgtcaca
    34381 ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg aaaagtcatc
    34441 ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac tatccaaacg
    34501 tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt gttagaacaa
    34561 ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac gacggatcaa
    34621 gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa aaacagcaaa
    34681 gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag taaaataaga
    34741 aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa gaaggtcaaa
    34801 gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa ctaagccaaa
    34861 ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat tcagaaggtc
    34921 gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg aagaaagagc
    34981 tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat gggaagaagg
    35041 aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg atggatctag
    35101 aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca aaattggatt
    35161 atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact gaagacaaag
    35221 agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa agtaacatta
    35281 gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa gagcatttta
    35341 tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg gattgttttt
    35401 cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta tttttctttg
    35461 gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc aagcaaaagt
    35521 aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt tccttacgat
    35581 acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata gttctgtttt
    35641 aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag tctttaaagt
    35701 cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg tagaaatcca
    35761 aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat tcaatgagaa
    35821 tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa aagagctgca
    35881 agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc atttagccta
    35941 tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca ttttgtctgc
    36001 tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag agcaaatcga
    36061 agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca ataatttact
    36121 tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg acatgaaaga
    36181 ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga ttattgtctt
    36241 gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa tgatagttga
    36301 ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag agagaagggg
    36361 aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta cttgcagggg
    36421 tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa attacgacag
    36481 atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac tatagatata
    36541 tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag ggacaattga
    36601 ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat aaaggtgtca
    36661 cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa aaactaatta
    36721 aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa aagtatacgg
    36781 aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg tagaaaaagg
    36841 cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt tgaatatttt
    36901 atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag atgtgaccac
    36961 attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta ttttccaaga
    37021 tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc tttctttaca
    37081 aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac gtttatccat
    37141 ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac aacgggtcgc
    37201 cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc ctacaggtgc
    37261 gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga atacaaaaga
    37321 tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt gccaacgtat
    37381 tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc aaagccgtga
    37441 ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg gggagattga
    37501 ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac tacttggtgt
    37561 tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca atggcaagca
    37621 acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt ttccgattcg
    37681 gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat tcctttttga
    37741 tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa ggcagaaaaa
    37801 tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact ttagtgggtt
    37861 cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat cagttagatc
    37921 tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact gttttgtttt
    37981 ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc aaaaaaccga
    38041 ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat aaattcttgt
    38101 ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat atgatgaaac
    38161 acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc gtattaggaa
    38221 cttatttcat tttccattct gtgatcattt tctttttatc attactaaag aaatcagaac
    38281 agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt cgtattcgtg
    38341 aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg ggcgctttga
    38401 cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat tctggatatg
    38461 atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa ctttcaccaa
    38521 cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat aatgcaagtg
    38581 aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg ccgaagatca
    38641 cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag gaacttcgaa
    38701 gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa tcagaatttg
    38761 atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac ttccaagcaa
    38821 acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca tctttgcaaa
    38881 aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt ctattctctg
    38941 gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt gctagctgtt
    39001 tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa atgcttgaaa
    39061 agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta ggcgtgttat
    39121 tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa atgtttggtc
    39181 tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc ttcgtattgt
    39241 atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg aaaaagagat
    39301 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata gctcctcata
    39361 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
    39421 aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt tattagaata
    39481 tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt tccatgtata
    39541 ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc aatgaacaaa
    39601 attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca tctttgtctg
    39661 tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat gaaaaacgag
    39721 agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct tatatcggtg
    39781 cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta gatgatgaaa
    39841 cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt gcacctagct
    39901 ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca tcggtcattt
    39961 tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat gtagaagtac
    40021 ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc agtgaccgca
    40081 aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat ccagcacagt
    40141 caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc aataaagata
    40201 caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc caacgtccta
    40261 aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg ggcggcatcg
    40321 atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa ttgacaagcc
    40381 cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg atttttacag
    40441 caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat catggcggta
    40501 tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt ggagatgtca
    40561 ctctggaacc taaccaagta cgcgagtttg taacggaata caaaataaat ataaagaaat
    40621 agaataaatt aaaaagggag tgtgacagtg tcacatcccc tttttaattt attctatttc
    40681 tttaaaggca gatcaagcca gaatgatttg tctaaactta acaaggttag tacaggaaca
    40741 gttgcagctt taactgtacg agtagttgtt tctggtatct gtcttgccgt aaaattgttc
    40801 cacaaatccc agattgaagc gacacggaag atggaagaag cgttgttgac gcgttttatc
    40861 gttgaaaatt ctaatcctgt gattggcacg ccatctgttg tccatttgat tggcacatcg
    40921 aatcgcttag acatattgag gatttcagcg tttgcttctt cagttgttgc tgcttctttt
    40981 ttcggttcat catattttgt caggtcgtac gcttcgatga tcgcgttcaa tttttgtgaa
    41041 tattggcgat ctgtagcata acgtccttcc aaatatttgg ttgcttcttt gtagtctttt
    41101 gtttccgact tccaagttgg tttgtagaaa tcaggattgc cagtcagtcc atttttgatc
    41161 aagtcggcat agtcttccaa actttccttg taagaaggat atttacggaa ttgtgcgcgg
    41221 atttggtagc tttgtccgga actatcttgt tcaagtgtat tgaaggtcac gctgcttcct
    41281 ttatatgatc ctttgatacc aaaaaggtta tgatttggtt cgcttgataa taagctgttt
    41341 ccagatccac tttcaaggat agcttgagcc atcatgactg atgcgtagat tccttctttt
    41401 tgcccgattt cctgtgcatc atctgcaatc tttttcaaga actgatcagt tgtttggttt
    41461 tgactgaaat ggaacgagcc atctaatcca tctaaaatag atgagccgcc tgtcagcgaa
    41521 cctgaagcag caggtaaacc tgctaagttt actcggacag caaaatcagg cgtgaaatct
    41581 gaaatagaag ttactcgaac tttatctcct ggttgaggcg catggataaa ttgtccgttc
    41641 ccaatataga tacctacatg gtgagtaaca ccggaagaag gtccccagaa gacgaggtcg
    41701 ccaggttggg cctgacttac tggaatctgc gtacctgcat attgctgttc gcctgtccag
    41761 ccaccgatat aatgaccggt tgctttcata tatacgtaat aagtcaaacc agaacagtca
    41821 aaagtatttg gaccttttgc tccccataca tatggttttc caagatgctt gaatgcttct
    41881 cgaacgattg cttgttggac attcgttgta tttgtaggca atacagcatc aattggtgat
    41941 tttacaacaa aatcagaaga agcagaagat ggattctcta taccgctttg atttgctgta
    42001 tttggacgag atgaagcatt gttcgttccg ccattcgatg aaccattgtt tggatggttt
    42061 ggttctctct gagtatttcc attgttattc gatccagatg ggtttgtttg attcgaagaa
    42121 ttcccgctgt tcgaattatt gttattattg tttgaattat ttgaaccatt attattgttg
    42181 ttattaccca catctggttt gtttggcgtt tccggatctg gtgtactagg gacttcttca
    42241 ctaggatcag ttggtttctc tgttgaagta tccggtccag ctttaaattc tgcagtaata
    42301 gataaattgt tcgtacctac tgtaaaggtc tttgcatcat aatttacttc tttggcacca
    42361 ttaacagcga ctgaagcaat ttgttcacct tcagctggcg taatagtgta agttaccttc
    42421 gtttccgcta ataaaccttc taccgttcca ctttccgtaa tatccaaagg ttgctgttca
    42481 ggggaatccg ctgtcgaagc agtcagttgg atcttaccaa tcaaagaagg agcaattgta
    42541 agggaatatg tctcggctgg cttttcattt tctgatggat cagccggatc aatttcagtt
    42601 ggctcggtcg atcctgttgt tgagttagtt gttccagttg ggtcttctgt aaaagaatcc
    42661 gttgtttcac tagtagctga gctggatgta gtcgtatctg ttgtgactgt ttgatcagct
    42721 atttgattat ctacggcaaa tacggtattg gtcgacaaaa cagacggagc aatcagccaa
    42781 gtagcagcta gcaaacgtat cgttttttta tttttcataa taaactagtc cttttccttt
    42841 cattgatttt ctgtcttatt cagcattcat tagacatttt ttggaacaga taatcgtgtc
    42901 gaaaagcttt ttcttttcat aaatctgtct gtgtattcta aataaaacaa tttgtaaata
    42961 agcaacgagc attcattcgt ttttctttga cgttcatttt attgcataga ccacctttgt
    43021 ttggggataa acaagtgggg ataaaaatgg ttatgcatat gtaaattata ccaaagtttg
    43081 gaaagtatca caaaaattta taaaaataat gatcctaaaa agaaaaagac tgaaatatgt
    43141 gataaacatc tcatacttca gtctttttca ttaactattc ataagacatt aggtcttcat
    43201 caaacccgct aacgagagta gtgatcattg ctacgactag actaacagcc agtcctgcta
    43261 agtaccagaa aaaagtaccg gaaatgaatg caggcaatgt ggtcagacta ccaaatacaa
    43321 aagcgatcgc tttcacttgg gcaaatccca ttacagctcc accagcagct gcaccaatgc
    43381 aagcagcaac aaatggtttt ttcaatggga tcgcaattcc gtaaacagtt ggttcggtca
    43441 ctcccgcaag taaaatcgaa gcaaaagcag aaccggagat cgaacgggtt tttgaacgtt
    43501 tacttcgcag aaatacacct aaagctgcac cagccattcc aaaattggct gctcctgtca
    43561 acggaacgat tcggtcgaac ccataagcag agatattact gatcatgaca gggtttacag
    43621 cccattggat accaaatatt accaaaatat tccaaccacc agcgatgatt gcgccactga
    43681 tcggtccgtt aatatccatc atccaagtaa ttgcggcagc taaaccttcg ccaaggtaaa
    43741 cacccgcagg cccgaatacc attaacgtca aaggaaccgt gataactaat gaaagtaaag
    43801 gtacaaatac taattgcata ttttcgcgaa tatatttttt gaggaatttt tcaacaaaag
    43861 aataaaagaa aatacctaaa atcgccggca tgacagtaga cgtatactgc atcagtacta
    43921 caggaatccc cagaaaatca gtagctgctt tgccactgtc tgaaagtaaa gaggtcagat
    43981 tcggttcaat cagtgctgca gcaatgacag ccgcaatata tccattcact ttaaacgttt
    44041 tggcagcaga aaaagcaaga aagattggca gaaaataaaa gacggcattc gacgcagcag
    44101 acaaaatatg ataagttccg cttgtttcac taagccagcc aatcgtagtc gctaaaacaa
    44161 ccagaccttt taatatacca gaaccggcaa acagcggaat gatcggcgta aagatttccg
    44221 atatcaaacc aaatagtttt tcgatcggat ttagcttttc tttcggagct gaatgatcga
    44281 tttgacgcgt caattccttg taagcaggag ccaatccctg atccatgatt atttctgcat
    44341 atctatcgag taaaatgaaa tacttaatgg aagggatttg ctgttttaat ttttctgcat
    44401 tggctactgg ctgttttttc aatttgatac gaagccgagt cgcacatgga aggacagaat
    44461 caatattgtc ttttcctccc aactgttcaa tcagcatatg aatctgctcg gacagtacca
    44521 tctgttcagg attcattgtc gtttccatta gaagaccact ccactcacta atataatatt
    44581 ggcgtaaggt gtcatttcgc ctgtacgaat aaatgcatga cagttattca actctttttt
    44641 catttcttca tgtgggataa agctaatcgg tgtatctggt aacaataatt tgattttttc
    44701 caatatttcc gggttcattg tctttatttc ttccgccaat tcaatcgatt gtacctcaag
    44761 ttcttctaaa acgttttcta aaacagataa aaaacttgga agaccgtttg agacagccaa
    44821 atctattttt tcagtaccaa aggggacagg catgcctgca tccccgatac ttaatttatc
    44881 aaagtgcccc atttgtgcga ttactcgtga aatatctgaa ttgattactt ttcctttttt
    44941 catgtttgtt tcttcctctc attgttaatt ttagactgct gattgttcta aagcttcgcg
    45001 gtaagggata gaaggttgcg caccataaga ctgcacagca acagaagagg ctagatttcc
    45061 ataaaggatc gcttctttca agttactaag gtctggctga agtgtgctgc ttaatgcgcc
    45121 aataaatgta tcacctgcgg ctgttgtgtc aacggcatcc actttaaaag cagggacgat
    45181 accgtgttct ttttctgtat gatagaaagc gccggcactg cctagtgtaa tgattactgt
    45241 tccaatacct aattcatgga gtttttcggc tgctgcgacc aaactgttat gatctgtcac
    45301 tctgattcct gtaatgattt ctgtttctgt ttcatttggt ataatgatat ctgtcttttc
    45361 aagtagttca gtaggaatcg tttcacgagc tggtgcagga ttcaggatcg ttgtttttcc
    45421 tgcgtctttt gcaatagaaa aagctaggat cgtcgcatct aagctacttt caaactgagc
    45481 aataataaag tcactgtttg taatcagttg cttgtttttt aggacttctt gttcatggaa
    45541 agcattattt gcccctgcat ggatcaagat actattttct cctgctgcgt ctaccataat
    45601 cattgcttgt ccggttgtgg cgccttggat ttcagctatc ccagatgtat cgatgttttc
    45661 ttttgtcagt agatctaata actgttgtcc ttcgctgtca gccccaacgc ctccaataaa
    45721 agatgtccgt gcgcctgaac gtttggctgc cactgcttgg ttggcacctt tacctccgcc
    45781 agcgtgaaaa atctcatgcg catgcatcgt ttcgcctggc ttgggcatgt tggttaaacg
    45841 aagggtagtg tccatattaa tacttccaat tactgtaacc gtgttcattt tacaaacctc
    45901 caactatctc atttattttg atgggaaaat tattttttaa aacgttttag taaaacgttt
    45961 taatagttcg ataaaaatat agcacacctt gaaagctatt tcaagggtgc agttgaaaat
    46021 cttttttcta atcgtacagg taatcttttt tcttcccatt ctttttctgg gaattggatc
    46081 cgatctaaca ataacttggc agatgtttgt cccaattcga agattggttg tgcgatcgtt
    46141 gtcagtttcg gcttgatata ctcgcacata tctatattat catagccaat aatgctgtag
    46201 tcttcaggga tgctttttcc agcttcctct aatccgcgat ataatccaaa agctaattca
    46261 tcatttaaag caaacacacc agttgcagca gaatcaagaa gttctgctgt gatctggtag
    46321 ccaccttgtt ttgagaactg tgtaggtaag aggatcagtt ggtcgtgagg gatttggtaa
    46381 acatcaagag cagatttaaa cccctcaatc cgagcgtgga cattttccgg tggattttca
    46441 ggatagacaa gggcgatcgt ttgatgtcct agactaagca agtgcatgcc cgctaggtaa
    46501 ccaccgcgaa aatcgtctgt tcgtacggca tcactaaatc cttcagattt tttctgatcc
    46561 aaaacaatga agggccggcc ttgttttttc aaattttcgt tgattgcatc tgtagagaca
    46621 gcagaagtcg cgataatgaa tccatccaca cctcgacgtg tgagctcggc tagatattcg
    46681 atttcttttt gatgatcaga atcagcatta cataatatcg tgacgaaatt ctgcttatac
    46741 aagatatcct caattcctcg catcagtgta ctgaagaatg gattcgtgat atcaggaacg
    46801 agtacaccaa ttgttttcgt ttccttggta atcatttgtc tagcaaaata gtcaggctga
    46861 tagcctaatt cttctttggc agctaaaact ttctgaacag ttttcggact gaatttctcc
    46921 gtatttccat ttaatatcaa ggagacagtt gtgattgaaa cgccggaaag tttagcgact
    46981 tctttgattg tgatcttttt tttcaccatg ttcatactcc tatctaaatc ctgtggttat
    47041 tttcccatga aatcaaaaag aagtaaatga aaatcacgtg tgccagtact tatttgcttt
    47101 gttggatagc ttcgattatt tgtttcgttg agcgtactcg tgcagttttc ggaaatatct
    47161 tttttatgaa caattgatgg gattctttgt cacgatcact gcaagcatct tcagcaagca
    47221 cgatatggta cccgtgttga aaggcatcta atgcagtagc atatacaccg tttgttgtcg
    47281 ctacaccgct taaaatgata gtttcgattc cacgccggcg aagttgaaga tcaagagaag
    47341 taccaaaaaa agcacttgga ttgtattttg tgatagtcaa catattatct gtatctttca
    47401 aagaatcctt caaaagtaaa tcagtgaatt ctggaggtac agaagcagtc attccaccca
    47461 tatcagtttg ttgactcaaa taagggaaag tggagatgtc tacgttcact aaaactttca
    47521 aggcagcggt acgtttcaat gttcttacta acaaatcatt gtttgtcaga atagatgctg
    47581 cagtgtgtgg agagagtgta ccgttattta cgatctcttt ttgcatgtcg atcgcgacaa
    47641 aagcagtttt tgataaatca aaaatcatat tttcctttaa atcattcatg atgttccttc
    47701 tttcaaataa caatgttttt tatgtaagta aatcataaat cgtttatttg tcaactaaat
    47761 taaaggattc ctttctttag tctagagaaa ttcagtggta aaataaataa gtgtgagaaa
    47821 acgcattcga tgcatagata tagcgagaaa tatcgtgaaa atggaggaat cagagtgaag
    47881 tatgtcgaag taaaaaatga atacaaacga tacaaaatgg gagaaacgac cattacagca
    47941 aatgacgata tttctttttc aattgaaaaa ggcgaattag tgattatact tggtccaagt
    48001 ggagcaggaa agtcaacgat tcttaatatt ttaggtggga tggatacacc tgatgaaggt
    48061 cagatcatta ttgatgacac agatattgcg caattttcag ataaacagct tactgcttac
    48121 cgacgaacag atgtagggtt cgttttccaa ttctacaatc tcgttcctaa tctaacagca
    48181 aaagaaaatg tggaattggc aacggaagtt tctcctgatg cgcttgatcc tgtagaggta
    48241 ttgaggcaag tgggcctagc tcatcgattg aataactttc cgtcccaatt atcaggaggc
    48301 gaacagcaga gggtttcaat tgctcgtgca ctagctaaaa atcccaaact tcttttatgt
    48361 gatgaaccga caggcgcgct ggattttgaa acgggcaagc aagtattgaa attattgcaa
    48421 aacgctagca gacaacacgg caataccgtt ttgatcatta cacataattc tgctttagcg
    48481 cccatcgctg atagagtcat tcacattaat gatgctaaag taaggtctgt tgaattaaat
    48541 gatcatcctt catccattga tgagattgta tggtaggggg attttatgaa aacaagaaca
    48601 tatcttaaag caggttttcg cgaaattcgg caatctaaag gacgattcat tgcaatcatc
    48661 cttattgtca tgctaggaac acttttattt gtaggtgtca aaactgctgg acctgtcatg
    48721 caaaaaacga tggatcacta tgttggtaca gcaggacttt ctgatttgca aatcgtttct
    48781 actggtggct tgacacaaaa agatattgct caagcagaaa agatcccaga tgcaaaagta
    48841 gaaacaggaa aacagatcta ttattccaat actggcaaaa acgaagtgat tcaaatattt
    48901 tcttataaca aaaataacaa gcagaacaag ctacaattaa ctgatggaaa actcccggaa
    48961 aaaccaaacc agcttgtatt ggatgaaaaa gcggaagatg aagggtataa aatcggtgat
    49021 atttatcaga ttgattcaga tgaattgaaa gaaaaagagt ataccatcac gggctttgtc
    49081 cgttctccat tgttcatcaa caatttggag agaggatacg cgaacgtcgg aaatggtagc
    49141 gtcgattatt ttgtctattt acctgaatcc gtttttaaaa cagatattta ttctgtctta
    49201 tatcttgatt ttacaaacgt aaagaatttg aatacgtaca gcaaagctta caaagataaa
    49261 atggagcaaa atcaagaaaa ggcagaaaag tatttacaag atcgacccga agaacgctta
    49321 gaagagctga aaaaatcggc caacgaagaa ttagaaccgg cacagcaaaa aatagcggat
    49381 ggcaaagcac aaacaacgca agcaagaaca cggttagaag atgctaaaaa acagttagcg
    49441 caacagtcag caactatcca gcagcttcct gaagcgcaac gaacaatcgc tgttcaaaca
    49501 ttatctcaac aagaagctca acttgctgaa caagaaaggc aattagttga gaaagaacgt
    49561 gaactggcct ctgcagagga agaattgaat cgaaatgcag aaaaaattga aaatttaact
    49621 aaaccatcat acctatacaa tgaacgttca gaaaatgtcg gttttcaaga atttggtgat
    49681 ctgtctgatc gtatcgctgc gattgccaat gtttttcctg ttttcttttt ctttattgcc
    49741 gcattgatca cctttacgac aatgacacgt atggtagaag aaaaccgcag ggaaatcggg
    49801 acattaaagg cgttaggtta tacgaaagtt gaaatcgcaa aaaaatatgc aatctatgca
    49861 agtctagcct cagggatagg tattgtttta ggaacgatat taggaacgaa tcttttgcca
    49921 aggatcattt ttgagctgtc taacgagcgg tatgatattg ggagtgcgct tattttttat
    49981 gactggccgc cgattttaca agcagctgcc gcattcttca ttgccgcatt tggcgctgca
    50041 atgatcgttt tattcaaaga tttaagagaa cggcctgctg cattgcttca accgaaagca
    50101 ccgaagccag ggaaacgcat ttttcttgaa tacatcactc cgctgtggtc acgccttagc
    50161 tttaatcaaa aaatcagtta ccgaaatctc ttccgatata agtcaagaat gcttatggcg
    50221 attatcggaa ttgctggatg tgccgggctg atggttgcag gcgtgggatt gaaagattct
    50281 ctaagttctg tttcagccaa acaatttgga ccgatcattg actatcaagc aatcgttaca
    50341 ttggattcat ccacggaaga tagtcgtcaa aaagcagcgg atatgctgca gaagcaagaa
    50401 aaagtaaatg agatcctagc tattcatact caaaccgtag aattgagaaa aaaaggacat
    50461 gcttcgcaaa gtctgacagt gatcgtacca agtaaaacag ctgatttgga tcgttttatc
    50521 catttaactg atcttgaaga gaacccacgc tccttgccta aaacaggaat cgctattacg
    50581 caaaaagtag cagaactatt tgacttatct acaggagatc atatttcgct ttatgatgaa
    50641 gatcaaaaag aatggaaagt cgaagtaaaa gaaatcgtga aaaactatct aggtagcttt
    50701 atttatatgt cccctgaata ttatgcttca gttactgatc aatcgatgat ggataatgca
    50761 tttttgctgc gtacagataa gatgacaaca aaagaagaag aaatgctttc cgagaaactg
    50821 cttgccacag acgctgtgac aaatacgtct ttcatgtcca aacaaatcga aacgcaagaa
    50881 gaatcaatgg cgaacttaga ctcggttgtc ttaatctttg tcgtattatc aggactgctg
    50941 gcttttatcg tcttgtataa tctaactaat atcaatattt ccgagcgtgt acgcgaatta
    51001 tctacaataa aagtccttgg attttttgat aaagaagtca cgatgtacat tgttcgagaa
    51061 aatatcatct ttactctttt aggaatcatc ggaggatttg gtgtaggata tatattgact
    51121 gacttcatct tgcaacaagc gtctatggag aatgtcatct ttccattagt gatcacatgg
    51181 tgggcttatg tcttatcagc aggattaact atcgtattta cggttattgt gatgattgtt
    51241 acccatttca aattaaagca tatcgatatg attgatgcgt tgaaatcgaa tgaataaaaa
    51301 cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg caaaatagtt
    51361 tttcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccatgaat
    51421 cagaggttgt aaaaaagccg tcctgcatcg agcaaaaaga atactcaaat aaaattagct
    51481 cctcatgttt tacatttgag aagactgatt gccgtagata aaagcttttg aacaccattt
    51541 atagaagaaa caagaatatt ttgctatttt aaacaagagg atagacaaat acatgtctga
    51601 tctcttgttt tttattgggc aacagacctc ttagaatgta taaagataaa ttttacaaac
    51661 gctttcggtt cagttataat aataaatgat aacttgtata tataagttgt ggtaggtaat
    51721 aaatcagttg aatggaggta aatatccatg acatttaaag acaaagtcat ttaccaaatc
    51781 tatcccaaat cttacaaaga cagtaatggt gatggaattg gagatttaaa aggaatcaaa
    51841 gaaaaactcc cgtatctgaa tgatctaggc atcgacatga tttggctaaa ccctatttat
    51901 ccaagccctc aaaaagacaa cggttatgat atctctgatt acacagccat cgatcctatc
    51961 tttggtacga tggaggaatt cgaacagcta atagccgaag caaagaaata ccggatagaa
    52021 atcatgctcg atatggtgct gaatcacgtt tcgacagaac atgaatggtt caaaaaagca
    52081 ctagcagggg accagcatta tcaggacttc ttcattttaa gagaagagcc aacggattgg
    52141 gtatcaaaat ttggtggaag tgcttggtcg ccatttggag atacggggaa atattatttg
    52201 cacttatatg atcggacaca agctgacttg aactggcgga accaagcagt aagagaggaa
    52261 ctattcaaag ttgttcgttt ttggatggaa aaaggggtaa aaggattccg tttcgatgtt
    52321 atcaatgtta tcggtaaaga cgaacaattg aaaaataata aagaaaatga tggcaaacct
    52381 gaatatacgg acaaaccaat cacccatacg tatctaaaag aattgaacca agcaacgttt
    52441 gggacggatt ccactatcat cacagtcggt gaaatgagct ctacgacgat cgaaaattgt
    52501 atcttgtata cagaacctag tagagaagaa ttatcaatgg tctttaattt ccatcatttg
    52561 aaagtcgatt atgaaaatgg gaataaatgg tctactcctc catttgattt tgcagaatta
    52621 aagcgactat ttcatacatg gggagaaaag atgagtgagg gtaacggctg gaatgctcta
    52681 ttttttaata atcatgatca gccacgtgcg ctcaatcgat ttgtagctat cgatcagtat
    52741 cgaaaacaag gagcagagat gctagcaacc atgatccatt tgaaccgtgg gacgccctat
    52801 atttatatgg gagaagaaat cggcatgatc gacccagact attcatcgat ttccgactat
    52861 attgacgtag aaagtttgaa tgcttataag ttactccttg aacaaggatt cacagaaaaa
    52921 gaagcgtttc gtcggatcaa agcaaaatct cgcgataact ctcgaacacc catgcaatgg
    52981 tcagacacag aacaagcagg atttactaca gggaaacctt ggttaaaagt tggaggcaag
    53041 ctagaagaga ttaatgtaga aaaagaacta tcttctgaag attctatttt gtcctattac
    53101 aagaaattga ttcggctgag aaaaacatat ccaatcgttg cacaaggaga ttatcatgct
    53161 tatgaagcaa atcatccgga agtttatggc tttcttcgcc aatttgaagg acagcagctg
    53221 cttgtattaa caaatttcta tgataaagat acaaggatcg ccatcccaga aggattcaca
    53281 gaagccaata ttcttatcga taattattcc gatactgttc ttcatcaaag tctggagcta
    53341 aaaccatacc aggcaattgc tttgctgaaa aattaaaaca aaaacacaaa acgtcgttcc
    53401 tacgagctat tctcgtaaga tcgacgtttt gtgttttatt taaaataatc ttcttcagaa
    53461 aatgaagagt tttcatactc tcctttatcg ccataaaatt tattgtagcg ttgtttgaat
    53521 ttacggaaaa taaaagtaac gagtaatttg actaacgtat agataggaat accaaaaata
    53581 acgcccacaa ttcccattag atcacccatg acaagtaaaa cgattaagat cgtaatcgga
    53641 tggatctgta aacgatcacc cattactcga ggaatcacca agtcaccgtg gagaagttgt
    53701 acggcaaacc agacaatgac aaatttgaca gccataaaag tagagtcttg gaatgcaatg
    53761 atcaaacctg gaaagaaggc gataaacggc ccaatatatg gaatgataca taacaaccca
    53821 gcagaaatag ctaaaacact agcaaattct aatccgatca gtaagaaaca aacccagtag
    53881 actgctccaa ggataaatga agcaatgatt tgccccttta gaaatgaacc caattgttga
    53941 ttagcaatct ttgtcaaatt tttaaaatct gtacgaaatg ctggtgggac aatcgctaaa
    54001 actgactgat aaaatttttg aggatctctc aaaaggaaaa aagcgacaat tggaccagta
    54061 aataaagtaa taaaaatagc tgtaacagtt gaaaaaatgt tgctgatccg ttgtgcgcca
    54121 ctttcccaat aatttccaat aaatccagct atatcagacg tcacgtcgtc aatagaagcg
    54181 aaaaattgat taaatgaatc ggcaaaaggt gttttctcca aaaattgtcg gagactctct
    54241 tgaaaatctt gaaacaaatc tggcaaacta ctgatcaatg tctttgcctg ttcttcaaga
    54301 aaaggaataa gccatatgct tccaagcccc aataaaaata aaataatcac ataaacgata
    54361 atgactgaca agttcctcga aaggtgcttg ctcaaacgtt tgatgactgg ttccaaaaga
    54421 taatacaaga tcagtgcaaa caataatggg ggcaagatag tagcgataat gattccgaca
    54481 ggcttgaata taaacgagat ctgatggaaa aaataaatga tcagcgcaag taagataact
    54541 attcctaaaa catagtaaga ggtttttcct cctaaaaagc ggatcaaacg attcgttgta
    54601 tctgtttctt ttttcactct ttttcctcct ttatctataa cttcattata gacaaaagcg
    54661 ggtataaaag gaaaatgata gaggttgatt gcaaaaatat cgtcaatcat tcatataaac
    54721 tttctcatca attaaaataa gaaaacgctt gacagttctt ttaaccatat gataaattaa
    54781 atcctgttgc taaaacgtaa ctattacggt ttgcgaaaag cgaatcggaa cattcaaatg
    54841 aaagaagatg agaagaggag tgtcataaat gaaacgattg ataggtatat tggccatgct
    54901 ggttttagca ggtatgttaa ctgcttgcgg ggcaagtggg aaagccgagg acagtaaaga
    54961 aaaattgagc gtgatgacga ccttttatcc catgtatgat tttacaaaag caatcgtcgg
    55021 ggatgaagga gaagtagagt tattgattcc agcagggact gattcccacg attatgaacc
    55081 ttctgctaaa gacatggcaa agatccaaga tacagatatc tttgtttata atgatgaaaa
    55141 catggaaaca tgggtccccg caatccaaaa aacattacaa gaaggcaatg tccatacgat
    55201 aaaagcgaca gaaggtatgc ttttacttcc gggaagtgaa gaaggacatg atcatgacca
    55261 tgaacacggg gaagaaggcc atacacatga gctagaccca catgtatggc tagctccaag
    55321 tttagcaatc aagcaggtag caaacatacg tgatcagctg attgaagctt acccagaaaa
    55381 acaggaagta tggacaaaaa atgcagcagc ctataccgag aaacttcaag cactccatca
    55441 gctttatcaa gaaaccttta agcaagcaaa acaaagatct tttgtcacgc aacatgctgc
    55501 attcaactat ctagcgttag aatatggatt gaatcaagta tcgattgctg gtctttcttc
    55561 tagtgaagaa ccgtctgcgg cgagaattgc tgaactgaaa cactttgtta aagaacatgg
    55621 aatcaactat atttattttg aagaaaacgc caaagatagt attgccagaa cgttagcaaa
    55681 cgaagcaggt gtatctttag aagtattgaa tcctttggaa ggattgacga atgaacaaat
    55741 agaaaatgga gaaaactatc tttctatcat ggaagcaaac ctcgaagcgt tgaaaaaaac
    55801 gacagagaca gaaaacccgc tagaagatac tttgaccccc aaaaaagaaa agagcgtcta
    55861 caacggttat tttgaagacg aggatgtaca agatcgctct ctttcagatt gggcaggaac
    55921 ttggcaaaca gtagatacgt atgtagaaga tgggactttt gatcctgttt ttgaatataa
    55981 agaaaaattg aatcaggata agacagcaca agaatataaa gaatattata cgaagggcta
    56041 tcaaacagat attgcttcaa tcaagatcag tgagaaagag atggcattta cgtttaagaa
    56101 tggaacaacg aaacgttcaa attatcgtta tgtagggaaa gaaatcttga cctacgaggc
    56161 aggaaaccgt ggcgtaaggt atctattcga agcagaagat aaggaaagtg gcgcttttag
    56221 atatattcaa ttcagtgatc acacgattgc accggctgac tccgaacatt ttcatttata
    56281 tgcaggaaat gaaagtcaag aagtattgct gaaagaaatg gagaattggc caacatttta
    56341 tccagataca atggatggac gtgaaattgc acaggaaatg cttttgcact gataggcaaa
    56401 aaagaaccga agggtttgga tcggctggtc atttagtcgg tccaaactat tcggttcttt
    56461 ttatgaaatc aatttcttta atccacggat agaggctggg atggtctcgc cgtttgccaa
    56521 cgtgacttct ctggtttttt tattaatcga ccgcacattt tgtgtattga taacatagga
    56581 tttatgacaa cggataaaat tccgatgcat tttctcgatt tctttgatct tctcatagaa
    56641 ttgtaattgg gcatgagtca aatggacttc cagtttatgt ggcgctccta gcgaagtaga
    56701 aaaatattgg atgtctttga tcggtaaaaa tttgatctca tcattaaact ctattttgaa
    56761 gatatctttt tggatttggg acatttgaaa atcttcgaaa attttgctca ggatatgctc
    56821 gacattttct tttaaatcat aaagatcttc tttgacgata taatcagtcg gttcgatgtt
    56881 gctttttaag attttcatcg acatttccgt atgggaagtc acatagatta tttttcctaa
    56941 aggatctaat tcacggatca atttaccgat cgctacacca tccatttcac tgtcttctag
    57001 ttcgatatct aaaaagtaaa taccaaaaga agaagttctg atcttggctg cattgagaag
    57061 ttcttccgga tcagaggtag aaatatggac ataactgtct aagttttcaa tcatgattct
    57121 atttttgatc accgttgata aaacttctaa ttgttttgga tcgtcttcac aaatataaat
    57181 gggaatcata tcattactcc tttattttat ttgtatataa atcgtttgag aaaaacgctg
    57241 gttttcaatt gatgtttcaa ggaaaagata aggattggtc tttgtcaatt catccaagat
    57301 aaacaagcca aggccttcgt tttttggatt tgtttttgtt gagaaccctt tttgtttcaa
    57361 ctggtttaac ggtaatgtat ttgttgttgt attgatcaca cgaatcatca cggaatgatt
    57421 cttcttgagt atggtcaatt cgattttttt cttctttgca gcactagctc cttcaatcgc
    57481 attgtccaag ataataccaa gcatacgtac aaggtttacc gtatgtttcg tatccagttc
    57541 gattggctca ggaaaatgaa gctggacgat caaccctttt tcctgtgcca tcatgagttt
    57601 taatgacagt aaactacgga tctcaggaac tttcatatta tccaacatac ttagattggc
    57661 cggaaaaagg ttcatcatct ggcgtgtagg taatatcgtt tgatcataat aagaccgtaa
    57721 tccttctaag tcgccttctg aaatatagct gtgaagagaa aaaagcagat tttggtaatc
    57781 atgtttgaat tctctcaagt cttgatagtt tttttctaga tctttgatat aatcctgttg
    57841 catcctgact tgttgctcta ataactgatc agctaatttc ttattagaga ttttccaata
    57901 ataaaccaag atgatcaggc caacacacag atatagaatg aaaaaactcg tattcaaagc
    57961 taagacatca ggtgtttcac cagaaaaacg
//
LOCUS       DACDHJ010000003        49698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDHJ010000003 DACDHJ010000000
VERSION     DACDHJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.003"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            291..1430
                     /locus_tag="IV223_000109"
     CDS             291..1430
                     /locus_tag="IV223_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8710424.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="IV223_000110"
     CDS             complement(1497..2072)
                     /locus_tag="IV223_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8710425.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="IV223_000111"
     CDS             complement(2077..3051)
                     /locus_tag="IV223_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8710426.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="IV223_000112"
     CDS             3334..4302
                     /locus_tag="IV223_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8710427.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="IV223_000113"
     CDS             4415..4693
                     /locus_tag="IV223_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8710428.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="IV223_000114"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="IV223_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8710429.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5159..5905
                     /locus_tag="IV223_000115"
     CDS             5159..5905
                     /locus_tag="IV223_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8710430.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="IV223_000116"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="IV223_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8710431.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="IV223_000117"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="IV223_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8710432.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="IV223_000118"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="IV223_000118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8710433.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="IV223_000119"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="IV223_000119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8710434.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="IV223_000120"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="IV223_000120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8710435.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="IV223_000121"
     CDS             10642..11184
                     /locus_tag="IV223_000121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8710436.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="IV223_000122"
     CDS             11207..12763
                     /locus_tag="IV223_000122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8710437.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="IV223_000123"
     CDS             12777..13679
                     /locus_tag="IV223_000123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8710438.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="IV223_000124"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="IV223_000124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8710439.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="IV223_000125"
     CDS             15122..15544
                     /locus_tag="IV223_000125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8710440.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="IV223_000126"
     CDS             16013..16243
                     /locus_tag="IV223_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8710441.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="IV223_000127"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="IV223_000127"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8710442.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="IV223_000128"
     CDS             17654..17830
                     /locus_tag="IV223_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8710443.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="IV223_000129"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="IV223_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8710444.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18792
                     /locus_tag="IV223_000130"
     CDS             18325..18792
                     /locus_tag="IV223_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8710445.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            18877..19761
                     /locus_tag="IV223_000131"
     CDS             18877..19761
                     /locus_tag="IV223_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8710446.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19878..20111
                     /locus_tag="IV223_000132"
     CDS             19878..20111
                     /locus_tag="IV223_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8710447.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="IV223_000133"
     tRNA            20199..20279
                     /locus_tag="IV223_000133"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="IV223_000134"
     tRNA            20286..20357
                     /locus_tag="IV223_000134"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
     gene            20872..21579
                     /locus_tag="IV223_000135"
     CDS             20872..21579
                     /locus_tag="IV223_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8710448.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21638..22272)
                     /locus_tag="IV223_000136"
                     /pseudo
     CDS             complement(21638..22272)
                     /locus_tag="IV223_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
     gene            22399..22686
                     /locus_tag="IV223_000137"
     CDS             22399..22686
                     /locus_tag="IV223_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710449.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            22794..23114
                     /locus_tag="IV223_000138"
     CDS             22794..23114
                     /locus_tag="IV223_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8710450.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23195..24508
                     /locus_tag="IV223_000139"
     CDS             23195..24508
                     /locus_tag="IV223_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8710451.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            24505..25224
                     /locus_tag="IV223_000140"
     CDS             24505..25224
                     /locus_tag="IV223_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8710452.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            25352..25903
                     /gene="raiA"
                     /locus_tag="IV223_000141"
     CDS             25352..25903
                     /gene="raiA"
                     /locus_tag="IV223_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8710453.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26125..28659
                     /gene="secA"
                     /locus_tag="IV223_000142"
     CDS             26125..28659
                     /gene="secA"
                     /locus_tag="IV223_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8710454.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            28785..29886
                     /gene="prfB"
                     /locus_tag="IV223_000143"
     CDS             join(28785..28847,28849..29886)
                     /gene="prfB"
                     /locus_tag="IV223_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8710455.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            29991..30677
                     /gene="ftsE"
                     /locus_tag="IV223_000144"
     CDS             29991..30677
                     /gene="ftsE"
                     /locus_tag="IV223_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8710456.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30661..31554
                     /locus_tag="IV223_000145"
     CDS             30661..31554
                     /locus_tag="IV223_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710457.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31706..33154
                     /gene="cls"
                     /locus_tag="IV223_000146"
     CDS             31706..33154
                     /gene="cls"
                     /locus_tag="IV223_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8710458.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            33491..34348
                     /locus_tag="IV223_000147"
     CDS             33491..34348
                     /locus_tag="IV223_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8710459.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            34463..35383
                     /gene="pstC"
                     /locus_tag="IV223_000148"
     CDS             34463..35383
                     /gene="pstC"
                     /locus_tag="IV223_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8710460.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            35383..36267
                     /gene="pstA"
                     /locus_tag="IV223_000149"
     CDS             35383..36267
                     /gene="pstA"
                     /locus_tag="IV223_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8710461.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            36282..37088
                     /gene="pstB"
                     /locus_tag="IV223_000150"
     CDS             36282..37088
                     /gene="pstB"
                     /locus_tag="IV223_000150"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8710462.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            37104..37862
                     /locus_tag="IV223_000151"
     CDS             37104..37862
                     /locus_tag="IV223_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8710463.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            37875..38552
                     /gene="phoU"
                     /locus_tag="IV223_000152"
     CDS             37875..38552
                     /gene="phoU"
                     /locus_tag="IV223_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8710464.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            38803..39821
                     /locus_tag="IV223_000153"
                     /pseudo
     CDS             38803..39821
                     /locus_tag="IV223_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            39818..40585
                     /locus_tag="IV223_000154"
     CDS             39818..40585
                     /locus_tag="IV223_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8710465.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            40685..42253
                     /gene="liaX"
                     /locus_tag="IV223_000155"
     CDS             40685..42253
                     /gene="liaX"
                     /locus_tag="IV223_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8710466.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYNHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            42553..42870
                     /locus_tag="IV223_000156"
     CDS             42553..42870
                     /locus_tag="IV223_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8710467.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            42870..43220
                     /locus_tag="IV223_000157"
     CDS             42870..43220
                     /locus_tag="IV223_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8710468.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            43387..44325
                     /locus_tag="IV223_000158"
     CDS             43387..44325
                     /locus_tag="IV223_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8710469.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            44340..45170
                     /locus_tag="IV223_000159"
     CDS             44340..45170
                     /locus_tag="IV223_000159"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8710470.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            45214..46239
                     /locus_tag="IV223_000160"
     CDS             45214..46239
                     /locus_tag="IV223_000160"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8710471.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            46254..47192
                     /gene="galU"
                     /locus_tag="IV223_000161"
     CDS             46254..47192
                     /gene="galU"
                     /locus_tag="IV223_000161"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8710472.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            47317..47757
                     /locus_tag="IV223_000162"
     CDS             47317..47757
                     /locus_tag="IV223_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8710473.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            47760..48362
                     /locus_tag="IV223_000163"
     CDS             47760..48362
                     /locus_tag="IV223_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8710474.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(48421..49527)
                     /locus_tag="IV223_000164"
     CDS             complement(48421..49527)
                     /locus_tag="IV223_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8710475.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT        16396 a         8653 c        10764 g        13885 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaatggc tcatacggaa tggcgcaaaa
    11401 cttagaatca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gaaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 acgggtagtt aatccgctag gtcagccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcaa tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag cgccgttgct ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagag ttcatgtaca acggtaaaca tgtcttgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag caaaattaag
    12121 tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat ctgtatctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgcctaga
    12361 cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact tggatgcagc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaattgca
    12481 tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac atggattctt
    12541 agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaagtaa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt taagaaaact ggttatatcg tcatcactgc agatggcggt ttagtcggtg
    13081 gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt tccaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc tcgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aaaaagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaattgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtaggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataaccgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattactc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat accacaatcg taagctctcg ttagttcgag agctcttttt
    20401 tttgctttgc taccttgttc cttgaagatt ctgctgaatt aagatagagt taagttaact
    20461 acaagaattg gagggatcca catgaaatgg aaagaaaaag tagacgaagc agctgaaaag
    20521 ttatatgatt tggttaaatc agaaaaatac aatattgaag tgaatattcc gaaaaaaggc
    20581 ggaaaagcgg tccaggtaaa atcaaaacgc ccaacgaatc atacaaaaaa atggatggct
    20641 aaaaaccgct aataataaca aaagatccat aagtcggcat tttcatcgat tttagggtct
    20701 tttgttattt tctaggttga acatatctaa aaaattgttt ctgacaatag gagtagggag
    20761 aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc tttatctttt tcagttgtga
    20821 atcaggtata taccagttat aatagaagaa acgaaaggac gggtgtacgt tatgtcgaat
    20881 caattacctg tatatataca gattcatgac caattaaaaa atgaaatcga acaaggtata
    20941 tggaaagtcg gagaccgtct tccttcagaa agagaattag cagtgaaatt caatgtcagc
    21001 cgtatgacgt tgagacaagc aattcaaaac ttggcagatg aaggaatctt agaaagaaag
    21061 atcggttcgg gtacgtatgt tgcgcgagaa aaagtacaag aaaaaatgtc tggtgcaaca
    21121 agttttacag aaattatgga atcacaaaat cgagtaccgt ctagtcggac aatctcttat
    21181 ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa acttgagtaa agatgacacg
    21241 atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc ctatttgttt tgaagttgcc
    21301 agtgttccac aaaagctgat cgatggctac agcaaagcag agattactga atcgttgtat
    21361 cgcacgctag aagaaaaagg cgggaaaaag atcggtccag cgaatcaaac gattactgct
    21421 gtgttggctt ctgagaaaat tgctgactat ttagaggtaa aacgaggaga tgcagttctt
    21481 cgtttgcgcc agatttctta ttttgaagac ggtacgccgt ttgagtatgt gcgtacgcaa
    21541 tacgtaggaa atcgttttga gttttatttg gaaaaataaa aaacaggtga tccctgattc
    21601 cttattagaa ataaaagaga atcagaatca cctgttttta tatatgatca gttatcagtt
    21661 gctcgtgata agaaacagaa ccctcttcaa attggacttg tccgttcagc agctcaatga
    21721 tggattcttt aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat
    21781 cagtatattg gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc
    21841 ctagttgcgg atacgtgaga tctcctgttg caaggtacct tctacgattc caatgtcaga
    21901 caatgcttga gagaccgcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
    21961 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
    22021 ttccaacatc ggtacgcccg ccgttccact tggttctcca tcatcgctgc ttcgctggat
    22081 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
    22141 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
    22201 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
    22261 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
    22321 taggttgttt caacactgta acggttgcgc aattgctgta gtatgcgata ataaagacaa
    22381 aggcaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
    22441 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
    22501 tttcccagta acgggcttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
    22561 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
    22621 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
    22681 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
    22741 aacagacgcg aagaaaacgt ttgcatagtt tgttttaaaa aggagagaaa agtatggcta
    22801 aaaaaacaat tatgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
    22861 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
    22921 acgcagataa caatcttgca aataaagacg tggatgtgtt gttattaggc ccacaagttc
    22981 gctttatgaa agctgatttt gagaaacgtc tggaaccaaa aggcattcct ttagatgtca
    23041 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
    23101 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
    23161 ttgttttttc gtatattttt ttagggcgaa gaatatgaat catttttggg gtagaaggct
    23221 tctgacgaga gatattgaag caatagactg tgaggaggta aatttaccaa attatgagaa
    23281 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
    23341 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
    23401 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
    23461 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tctcaaagag
    23521 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
    23581 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
    23641 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
    23701 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
    23761 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
    23821 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
    23881 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
    23941 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttacgctttc atcggcgttt
    24001 gcttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
    24061 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
    24121 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
    24181 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
    24241 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
    24301 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
    24361 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
    24421 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagtt tgtcaggaaa tcaaagaaat
    24481 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcttccaa
    24541 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
    24601 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
    24661 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
    24721 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
    24781 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
    24841 ctagcaggca cctttgcata tagatggacg cagataaaaa aagaataccc gacatacatc
    24901 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
    24961 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
    25021 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
    25081 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
    25141 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
    25201 cgaaccattt ctttggcgag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
    25261 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
    25321 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
    25381 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
    25441 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
    25501 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
    25561 ttctccagat ttatacgcaa gtatcgattt ggttgttgat aaattggaac gccaaatccg
    25621 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
    25681 tgctgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
    25741 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
    25801 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    25861 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    25921 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    25981 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    26041 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    26101 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    26161 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    26221 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    26281 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    26341 gcaaaacgtg tattaggttt atatccatac cacgtacaat tgatgggtgg aatcgtgctt
    26401 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    26461 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    26521 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    26581 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    26641 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    26701 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    26761 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    26821 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    26881 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    26941 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    27001 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    27061 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    27121 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    27181 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    27241 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccaaatt
    27301 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    27361 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    27421 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    27481 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    27541 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    27601 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    27661 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    27721 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    27781 cgaatcaaag ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    27841 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    27901 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    27961 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    28021 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    28081 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagcattact
    28141 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    28201 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    28261 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    28321 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    28381 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    28441 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    28501 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    28561 aaacagccaa tccatgtgaa taaggtcgga agaaatgatc cttgtccttg tggaagcggg
    28621 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    28681 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    28741 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    28801 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag tttcaggagg tctctttgac
    28861 ttagatcagc tggaagaaga catcgcggaa gcagaagcaa aaatggctga acctggtttt
    28921 tgggatgaca gcgaagccgc acaaaaagtg atcagcgaaa ataacgctaa taaagaaacc
    28981 tatgaccaat tccattctct tgccgaagaa ctagaagaaa tcgaattgat ggtggagatg
    29041 ctgcaagaag aacctgatgc agcaatgcaa gaagaagcag aaacacgtat caatgcatta
    29101 gatgaaaaaa tgaagacgta tgaattatct atgctgttag acggacctta tgatcgaaac
    29161 aatgcgatcg tcgaattgca tccaggggct ggcggaacgg agtcccaaga ctggggcagc
    29221 atgcttttac gtatgtatac acgttgggca gagcagcatg gctatcaagt agaaacacta
    29281 gattatcaag caggtgatga agcagggatc aagagtgtga ccctgctgat taaaggacat
    29341 aacgcttttg gttatctaaa atcagaaaaa ggtgttcatc gccttgtacg gatttcgcct
    29401 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    29461 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    29521 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    29581 cctacaggaa cagtagtagc tagtcaggca caacgctcac aattgaaaaa ccgtgagcaa
    29641 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    29701 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    29761 gttttccatc cctattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    29821 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    29881 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    29941 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    30001 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcgattg
    30061 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    30121 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    30181 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    30241 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    30301 tgcaagttat cggcagaaaa cctcgagata tcaaaaaacg agtaatggaa gtgctggacc
    30361 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    30421 aacgtgtctc tattgctcgg gcaatcgtga atacgcctaa agtacttatc gcggatgaac
    30481 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    30541 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    30601 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    30661 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    30721 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacgtt gactttagtc
    30781 ggtgtattca tggcagttat catgaatgca actaaattag ctcaggatat agaaggaaac
    30841 gttgatgtat ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    30901 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    30961 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    31021 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    31081 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    31141 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    31201 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    31261 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    31321 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    31381 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    31441 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    31501 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    31561 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    31621 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    31681 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    31741 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    31801 tcaaacatgg gcatggctgc tcgttttgat gttcattcca gttgtaggat ttattttata
    31861 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    31921 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    31981 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    32041 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    32101 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    32161 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    32221 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    32281 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    32341 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    32401 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    32461 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    32521 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    32581 ttttccgtcc attcacacta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    32641 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    32701 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    32761 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    32821 cgtttactgg gcaacgtatt ctttcgctgc agagctgatc gagtatggag caaggatcga
    32881 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    32941 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    33001 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    33061 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    33121 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    33181 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    33241 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    33301 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    33361 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    33421 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    33481 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ccattctttt
    33541 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    33601 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    33661 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    33721 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    33781 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    33841 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    33901 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    33961 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    34021 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    34081 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    34141 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    34201 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    34261 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    34321 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    34381 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    34441 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    34501 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    34561 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    34621 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    34681 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    34741 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    34801 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    34861 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    34921 ttttggcggg acagggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    34981 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    35041 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    35101 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    35161 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggac ttactactcc
    35221 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    35281 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    35341 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    35401 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    35461 gctttactac tttatatctt agttcgggga ataccgcata tttcatggtc cttcttgacc
    35521 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    35581 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    35641 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    35701 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    35761 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    35821 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    35881 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    35941 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    36001 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    36061 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    36121 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    36181 gccgtgttga tcattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    36241 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    36301 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    36361 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    36421 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    36481 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    36541 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    36601 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    36661 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    36721 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    36781 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    36841 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    36901 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    36961 ctcgtatcag cgactatact gcctttttct atctaggaaa agtaatggaa tatgacaaaa
    37021 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    37081 ttggttaaga cggaggcctc aacatgacaa aagaaattat ttcatctaaa gatcttcatc
    37141 tgtattatgg aaagaaagaa gcactaaaag ggatcaatct aagtttcaat caaggggaaa
    37201 tcactgcgat gatcggtcct tctggttgcg ggaaatccac ttatctgcgt tctttgaacc
    37261 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    37321 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    37381 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    37441 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    37501 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    37561 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    37621 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    37681 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    37741 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaac gatacgaaga
    37801 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    37861 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    37921 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    37981 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    38041 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    38101 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    38161 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    38221 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    38281 tagtagctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    38341 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    38401 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    38461 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    38521 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    38581 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    38641 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    38701 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    38761 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aaattagcat catcactata
    38821 ttttttattt gtgtgcttac ttcatgggtt attcagcatc aagctgcttt atatgaagta
    38881 cctgtcatcg agatcgaaaa agtacgaata aaagaggatc atcaattgat tacaggaaga
    38941 ctgatgaaca cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat
    39001 caaatagaaa cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat
    39061 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
    39121 attggcgcca tgttgcttat tggcggaaaa cagggtctat ttgctttagg aagtctcatc
    39181 atcaatatcg ttttacttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    39241 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttaa
    39301 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    39361 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    39421 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    39481 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    39541 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    39601 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    39661 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    39721 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    39781 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    39841 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    39901 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    39961 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    40021 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    40081 ttgtttccta ctattgcttc ctatcactga ttttttagcg atccaaggtt ttacatcggt
    40141 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    40201 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgctagtg tagcaatttc
    40261 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    40321 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    40381 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    40441 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    40501 gtctgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    40561 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    40621 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    40681 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    40741 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaataaaaaa
    40801 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    40861 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    40921 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    40981 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    41041 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    41101 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    41161 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    41221 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    41281 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    41341 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    41401 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    41461 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    41521 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    41581 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    41641 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    41701 taaacgagta tacaaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    41761 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    41821 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    41881 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    41941 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    42001 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    42061 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    42121 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    42181 agatggtgaa atcacgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    42241 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    42301 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagg ctatctcttg
    42361 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    42421 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    42481 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    42541 aggtgagaca tgatgaaaaa aaagttgaca aaatccaata aaaatgttgt tttgacaggg
    42601 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    42661 gtattcctga gcttagttgc ttttggaagt ccaatcattt tatacatttt attagctttg
    42721 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    42781 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    42841 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    42901 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    42961 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    43021 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    43081 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    43141 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    43201 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    43261 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    43321 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    43381 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    43441 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    43501 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    43561 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    43621 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc ggctccagaa
    43681 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    43741 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    43801 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    43861 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gtcatcgatt gatcgcagac
    43921 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    43981 ctggaacatc taattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    44041 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    44101 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    44161 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    44221 gtagccgcga tgaatttccg tgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    44281 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    44341 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    44401 cattgatcat cgtatcaggt atcattatcg ccatgtggct tagcagcaga gaggctgttc
    44461 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    44521 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    44581 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    44641 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    44701 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    44761 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    44821 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    44881 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    44941 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    45001 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    45061 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    45121 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    45181 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    45241 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    45301 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    45361 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    45421 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    45481 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    45541 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    45601 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc gaaacatgac
    45661 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    45721 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    45781 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    45841 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    45901 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gagtaggcga tctagtcgtt
    45961 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    46021 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    46081 gcagcgatgg aactagccca aataatggaa atcgaaatgc ccatcactca aacaatctat
    46141 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    46201 gggaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    46261 tcaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    46321 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    46381 aagcgttgaa atcaggaatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    46441 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    46501 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    46561 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    46621 cgtttgtcgt tatgctaggc gatgatatca tggaagatga agtaccattg acacagcaat
    46681 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    46741 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    46801 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    46861 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    46921 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    46981 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    47041 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtatatcc
    47101 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    47161 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    47221 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    47281 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    47341 ttgattgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    47401 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    47461 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttgct ggtcaaaagt
    47521 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    47581 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    47641 aaagtcggcg gaatcggtaa agcgacagct aaagcgacgg ttgccggaaa agtcggtggg
    47701 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    47761 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggaacagcc gcagcaatcg
    47821 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    47881 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    47941 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    48001 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    48061 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    48121 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    48181 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    48241 tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat gatgaaaatg gcaaaagaag
    48301 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    48361 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    48421 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    48481 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    48541 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    48601 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    48661 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    48721 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtata ttttttcttg
    48781 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    48841 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtgt ttcctggtgt
    48901 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    48961 aacaccttgt tttttcaatt ggtattcgat ttctgcaatg atcgcttgtt cagtcacgcc
    49021 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    49081 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    49141 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    49201 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    49261 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    49321 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    49381 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    49441 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    49501 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    49561 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    49621 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    49681 tagaaatgta agacgtat
//
LOCUS       DACDHJ010000004        46234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDHJ010000004 DACDHJ010000000
VERSION     DACDHJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.004"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1130
                     /locus_tag="IV223_000165"
     CDS             138..1130
                     /locus_tag="IV223_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8710476.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            1182..2051
                     /locus_tag="IV223_000166"
     CDS             1182..2051
                     /locus_tag="IV223_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8710477.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(2471..4036)
                     /gene="guaA"
                     /locus_tag="IV223_000167"
     CDS             complement(2471..4036)
                     /gene="guaA"
                     /locus_tag="IV223_000167"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8710478.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4095..5018)
                     /locus_tag="IV223_000168"
     CDS             complement(4095..5018)
                     /locus_tag="IV223_000168"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8710479.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5214..5819
                     /locus_tag="IV223_000169"
     CDS             5214..5819
                     /locus_tag="IV223_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8710480.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6127..7143
                     /locus_tag="IV223_000170"
     CDS             6127..7143
                     /locus_tag="IV223_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8710481.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7284..8585
                     /locus_tag="IV223_000171"
     CDS             7284..8585
                     /locus_tag="IV223_000171"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8710482.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            8631..9293
                     /gene="deoC"
                     /locus_tag="IV223_000172"
     CDS             8631..9293
                     /gene="deoC"
                     /locus_tag="IV223_000172"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8710483.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9314..9712
                     /locus_tag="IV223_000173"
     CDS             9314..9712
                     /locus_tag="IV223_000173"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8710484.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            9778..10860
                     /locus_tag="IV223_000174"
     CDS             9778..10860
                     /locus_tag="IV223_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8710485.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11072..12634
                     /locus_tag="IV223_000175"
     CDS             11072..12634
                     /locus_tag="IV223_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710486.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            12627..13760
                     /locus_tag="IV223_000176"
     CDS             12627..13760
                     /locus_tag="IV223_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710487.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            13760..14716
                     /locus_tag="IV223_000177"
     CDS             13760..14716
                     /locus_tag="IV223_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710488.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            14815..15981
                     /gene="deoB"
                     /locus_tag="IV223_000178"
     CDS             14815..15981
                     /gene="deoB"
                     /locus_tag="IV223_000178"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8710489.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16005..16823
                     /locus_tag="IV223_000179"
     CDS             16005..16823
                     /locus_tag="IV223_000179"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8710490.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            16840..17547
                     /gene="deoD"
                     /locus_tag="IV223_000180"
     CDS             16840..17547
                     /gene="deoD"
                     /locus_tag="IV223_000180"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8710491.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(17845..18531)
                     /gene="gpmA"
                     /locus_tag="IV223_000181"
     CDS             complement(17845..18531)
                     /gene="gpmA"
                     /locus_tag="IV223_000181"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8710492.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(18703..19284)
                     /locus_tag="IV223_000182"
     CDS             complement(18703..19284)
                     /locus_tag="IV223_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8710493.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(19417..20100)
                     /gene="rpiA"
                     /locus_tag="IV223_000183"
     CDS             complement(19417..20100)
                     /gene="rpiA"
                     /locus_tag="IV223_000183"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8710494.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            20419..20832
                     /gene="rpsL"
                     /locus_tag="IV223_000184"
     CDS             20419..20832
                     /gene="rpsL"
                     /locus_tag="IV223_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8710495.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            20916..21386
                     /gene="rpsG"
                     /locus_tag="IV223_000185"
     CDS             20916..21386
                     /gene="rpsG"
                     /locus_tag="IV223_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8710496.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            21480..23567
                     /gene="fusA"
                     /locus_tag="IV223_000186"
     CDS             21480..23567
                     /gene="fusA"
                     /locus_tag="IV223_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8710497.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            23739..24926
                     /gene="tuf"
                     /locus_tag="IV223_000187"
     CDS             23739..24926
                     /gene="tuf"
                     /locus_tag="IV223_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8710498.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            25333..25641
                     /gene="rpsJ"
                     /locus_tag="IV223_000188"
     CDS             25333..25641
                     /gene="rpsJ"
                     /locus_tag="IV223_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8710499.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            25672..26301
                     /gene="rplC"
                     /locus_tag="IV223_000189"
     CDS             25672..26301
                     /gene="rplC"
                     /locus_tag="IV223_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8710500.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            26329..26952
                     /gene="rplD"
                     /locus_tag="IV223_000190"
     CDS             26329..26952
                     /gene="rplD"
                     /locus_tag="IV223_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8710501.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            26952..27242
                     /gene="rplW"
                     /locus_tag="IV223_000191"
     CDS             26952..27242
                     /gene="rplW"
                     /locus_tag="IV223_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8710502.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            27281..28114
                     /gene="rplB"
                     /locus_tag="IV223_000192"
     CDS             27281..28114
                     /gene="rplB"
                     /locus_tag="IV223_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8710503.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            28157..28435
                     /gene="rpsS"
                     /locus_tag="IV223_000193"
     CDS             28157..28435
                     /gene="rpsS"
                     /locus_tag="IV223_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8710504.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            28458..28805
                     /gene="rplV"
                     /locus_tag="IV223_000194"
     CDS             28458..28805
                     /gene="rplV"
                     /locus_tag="IV223_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8710505.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            28819..29475
                     /gene="rpsC"
                     /locus_tag="IV223_000195"
     CDS             28819..29475
                     /gene="rpsC"
                     /locus_tag="IV223_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8710506.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            29478..29912
                     /gene="rplP"
                     /locus_tag="IV223_000196"
     CDS             29478..29912
                     /gene="rplP"
                     /locus_tag="IV223_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8710507.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            29902..30090
                     /gene="rpmC"
                     /locus_tag="IV223_000197"
     CDS             29902..30090
                     /gene="rpmC"
                     /locus_tag="IV223_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8710508.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            30115..30381
                     /gene="rpsQ"
                     /locus_tag="IV223_000198"
     CDS             30115..30381
                     /gene="rpsQ"
                     /locus_tag="IV223_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8710509.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            30439..30807
                     /gene="rplN"
                     /locus_tag="IV223_000199"
     CDS             30439..30807
                     /gene="rplN"
                     /locus_tag="IV223_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8710510.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            30844..31152
                     /gene="rplX"
                     /locus_tag="IV223_000200"
     CDS             30844..31152
                     /gene="rplX"
                     /locus_tag="IV223_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8710511.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            31180..31719
                     /gene="rplE"
                     /locus_tag="IV223_000201"
     CDS             31180..31719
                     /gene="rplE"
                     /locus_tag="IV223_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8710512.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            31738..31923
                     /locus_tag="IV223_000202"
     CDS             31738..31923
                     /locus_tag="IV223_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8710513.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            31959..32357
                     /gene="rpsH"
                     /locus_tag="IV223_000203"
     CDS             31959..32357
                     /gene="rpsH"
                     /locus_tag="IV223_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8710514.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            32389..32925
                     /gene="rplF"
                     /locus_tag="IV223_000204"
     CDS             32389..32925
                     /gene="rplF"
                     /locus_tag="IV223_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8710515.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            33086..33442
                     /gene="rplR"
                     /locus_tag="IV223_000205"
     CDS             33086..33442
                     /gene="rplR"
                     /locus_tag="IV223_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8710516.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            33463..33963
                     /gene="rpsE"
                     /locus_tag="IV223_000206"
     CDS             33463..33963
                     /gene="rpsE"
                     /locus_tag="IV223_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8710517.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            33978..34157
                     /gene="rpmD"
                     /locus_tag="IV223_000207"
     CDS             33978..34157
                     /gene="rpmD"
                     /locus_tag="IV223_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8710518.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            34201..34641
                     /gene="rplO"
                     /locus_tag="IV223_000208"
     CDS             34201..34641
                     /gene="rplO"
                     /locus_tag="IV223_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8710519.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            34641..35936
                     /gene="secY"
                     /locus_tag="IV223_000209"
     CDS             34641..35936
                     /gene="secY"
                     /locus_tag="IV223_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8710520.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            35998..36645
                     /locus_tag="IV223_000210"
     CDS             35998..36645
                     /locus_tag="IV223_000210"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8710521.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            36836..37054
                     /gene="infA"
                     /locus_tag="IV223_000211"
     CDS             36836..37054
                     /gene="infA"
                     /locus_tag="IV223_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8710522.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            37087..37203
                     /gene="rpmJ"
                     /locus_tag="IV223_000212"
     CDS             37087..37203
                     /gene="rpmJ"
                     /locus_tag="IV223_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8710523.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            37221..37586
                     /gene="rpsM"
                     /locus_tag="IV223_000213"
     CDS             37221..37586
                     /gene="rpsM"
                     /locus_tag="IV223_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8710524.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            37614..38003
                     /gene="rpsK"
                     /locus_tag="IV223_000214"
     CDS             37614..38003
                     /gene="rpsK"
                     /locus_tag="IV223_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8710525.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            38083..39021
                     /locus_tag="IV223_000215"
     CDS             38083..39021
                     /locus_tag="IV223_000215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8710526.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            39049..39429
                     /gene="rplQ"
                     /locus_tag="IV223_000216"
     CDS             39049..39429
                     /gene="rplQ"
                     /locus_tag="IV223_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8710527.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            39701..40540
                     /locus_tag="IV223_000217"
     CDS             39701..40540
                     /locus_tag="IV223_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8710528.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            40516..41385
                     /locus_tag="IV223_000218"
     CDS             40516..41385
                     /locus_tag="IV223_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8710529.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            41375..42172
                     /locus_tag="IV223_000219"
     CDS             41375..42172
                     /locus_tag="IV223_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8710530.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            42375..43121
                     /gene="truA"
                     /locus_tag="IV223_000220"
     CDS             42375..43121
                     /gene="truA"
                     /locus_tag="IV223_000220"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8710531.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(43576..>46234)
                     /locus_tag="IV223_000221"
     CDS             complement(43576..>46234)
                     /locus_tag="IV223_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8710532.1"
                     /translation="LKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSILASD
                     AFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPNQMM
                     PDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMWLDA
                     FFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWS
                     EYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKN
                     MIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVP
                     LNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAALFAV
                     AWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEAL
                     VNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSANDSI
                     NYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKN
                     ESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTL
                     SGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQAYLS
                     LFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTKEIS
                     FMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWK
                     AKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWY
                     LDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        14842 a         8499 c        10042 g        12851 t
ORIGIN      
        1 gttttgaagg ataatgtttt tagaaaagaa cagatagcta gatttcccat ggatttgcta
       61 ttagtatttt gaaaaaatta gtaatttatg acaaaattca ttatactaaa cttgtgcaga
      121 aatgtgaggg atcaactatg aaagcaacaa tgaaagatgt cgcaaaatta gcaggtgttg
      181 gagtaggaac tgtttcccgg gtattgaata acggttctgt taaagcttct actagagaaa
      241 aagttgaagc agcaatgaca gagttaaact atcaaccaga tttttatgcc cgggggttaa
      301 agatgaagca tacgaacacg gttgctttga ttctacccac ggtatggcat ccattttttg
      361 gcgaatttgc ctaccatgta gaaacagcat tagaaagaca aaactacaag ctttatttgt
      421 gtaactccag tggtaatagt gaaaaagaac aagaatatat ccagatggtc agccaaaata
      481 aagtagacgg catcattgga attacttatt cagatatcga tgaatacatt tcgagtaatc
      541 tgccttttgt tagtattgac cgttatttca ctgaagaggt gatttatgtg accgctgaca
      601 atgcggccgg tggacgtttg gctgcaaatg aactttatcg ccgaggatgt cagaatgtag
      661 catatatcgg tgggtaccaa gaaacaccga atgaaacgaa gagtcgtcga aaatattttg
      721 aaatcgagtg ccgtcaactg gggatttcct ataaagcgtt agatatgtta gaacctttag
      781 accatctctc agaacgtgta acaaattttt tgttagagaa tcccgatatc gatgggattt
      841 ttacgatcaa cgatccaatg gcattagctg tcattgatat gcttgaaaaa atagggaaaa
      901 acgtaccaga ggatgtccaa gttatcggat ttgatggtca aaagatgaca gaaggagatc
      961 cttatctagt ctcaacgatt gcccaaccta caaagcaaat ggcggaagtg gcagtggcaa
     1021 aattaattgc tgtcattaat ggagagacaa tagaaaaacg taccgtattg ccgattgaat
     1081 ttggcgaagg tgacacaacg aaacctttag tgagaaacga ttttgcttag tgcaaatacg
     1141 tttctttttt tgaaaatgag tgaatgaaag gtgtagataa aatgttatat ggtggaatag
     1201 aagccggtgg aacaaagttt gtctgtggga ttggtaatga acaattacaa gtgatagaac
     1261 ggaaaagttt tcctaccaca acaccagaag aaacgatgga gcaagtgttt gatttctttg
     1321 ataagtacaa aggaaatatc gcatcaattg gtgtaggagc atttgggcct attgatattc
     1381 aaatcgaatc aagaacttac ggatatatca ccaacacacc gaagttagct tggcaaaatt
     1441 atcattttgt aggagctttg gaaaacgcct tgtcggttcc catctattgg actaccgatg
     1501 taaatgctgc ttgttatgga gaatatatgg cagggtatgg aaaaggtaaa aatagtgttc
     1561 tgtattacac cgttggtaca ggtgttggtg gtggtgctgt agtggatggg aaaatagtcg
     1621 caggttttag tcatcctgaa atgggacata tgctcttaca acgtcataat gaagatcaat
     1681 ttgaaggaat ctgtcctttt catcatgatt gtttggaagg gttagcatca ggacctgcga
     1741 ttgaaaaacg ctttggaaga aaagcccaga ctatctcatt tgatcatcct tattggacga
     1801 ttgaggcaga ttatcttgct cagtgtgtgt ataacacaac gttactatat gcaccggaag
     1861 taataatttt aggcgggggt gtaatgaaac agccgcaact actcgagttg attcgccaat
     1921 cgtttaaaga aaaaatgggt tcctacgtgg atacgcctac tctcgaagaa tatctactaa
     1981 ctccaaggct tgctgacaat gcgggcttga ttggctgcct agctttagca ataaagaaac
     2041 tcaatcatta gaatagtatt tattagaact attcataaca taccggaagt ttaaaagggt
     2101 attctgaaac ttaaatagtc tcaattcatt cttctttctt gagaatctga gtagagactt
     2161 ttattctgtt tcaaattaat ataattacga aatgatattg agtacaagaa agttcttttt
     2221 accagatctt ttaaatcgaa aaatctgata tatatattta tagtactaaa aatctgatta
     2281 gataaaaagc taattcaata ggaaaataaa ggtagtgaat aaaagcaaag cccttgttag
     2341 tagttaatct aacaagggct ttggtaggga aaatggtagg aaaccatctt attttttacg
     2401 aaaatcagga actttagtaa ttgaataatg ttgatttata ggtattcctc atccatatta
     2461 tgttggctct ttattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga
     2521 tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc
     2581 atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga
     2641 ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa
     2701 cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga
     2761 tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa
     2821 taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact
     2881 gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa
     2941 attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt
     3001 ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt
     3061 cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga
     3121 ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg
     3181 cattgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg
     3241 cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg
     3301 ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga
     3361 cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata aagttatcca
     3421 ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc
     3481 cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt
     3541 cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa
     3601 ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa
     3661 acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag
     3721 caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta
     3781 atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata
     3841 cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg
     3901 cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga
     3961 tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat
     4021 cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa
     4081 actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt
     4141 ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc
     4201 aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata
     4261 gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa
     4321 cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca
     4381 aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca
     4441 acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca
     4501 tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa
     4561 tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc
     4621 tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt
     4681 cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg
     4741 gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat
     4801 aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca
     4861 agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata
     4921 ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct
     4981 tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca
     5041 tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg
     5101 atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg
     5161 gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa
     5221 atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt
     5281 taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg
     5341 attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa
     5401 agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac
     5461 agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg
     5521 caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc
     5581 atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg
     5641 tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg
     5701 tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta
     5761 atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac
     5821 tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta
     5881 tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga
     5941 aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca gaacttgtat
     6001 agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac tttccttttt
     6061 tctctattct tttcttgggg gttttggtac actgataggg aatttagata gaagggagcc
     6121 aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc agtaacaact
     6181 gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag aaaaaaagtg
     6241 ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa tgtgatcaat
     6301 cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt cttctccaaa
     6361 ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat gttatgcaat
     6421 tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca ccgctcagtg
     6481 gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt agacagcggt
     6541 ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg ggataacgga
     6601 cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat
     6661 ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt agaagaacgt
     6721 ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg gaaatttgtt
     6781 gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt actcaaacat
     6841 aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg
     6901 atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt cgatggatta
     6961 aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag ttttgatatt
     7021 ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag aaaagttccg
     7081 aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt gacaaagggc
     7141 taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg ctttttttat
     7201 ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt ataataccga
     7261 atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa aagagggatg
     7321 gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg aatggaaaga
     7381 ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac atgacaaatg
     7441 aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc gatttgagtt
     7501 ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac acgaccacat
     7561 tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg
     7621 gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga tttcagatcg
     7681 agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg gtaattggtc
     7741 aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga
     7801 ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc gcggcaggag
     7861 cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag aacatcgaag
     7921 atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac cgagaaacag
     7981 tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat agtctagaag
     8041 ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta gaaatgtgct
     8101 acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc gatgaagcaa
     8161 gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga
     8221 tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt ttgactgcac
     8281 gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg
     8341 aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg
     8401 atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca aaaggtgagt
     8461 cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa ttattatata
     8521 aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac atcatcacag
     8581 aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa atggaactaa
     8641 accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct gtgatgcaaa
     8701 ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca gcatgggttg
     8761 cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta atcgggtttc
     8821 ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc atcaataacg
     8881 gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac
     8941 aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag
     9001 ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc gaaatagcaa
     9061 aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc ggagcaaagg
     9121 tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc aaagcttccg
     9181 gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct acaagaatcg
     9241 gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat taattttttc
     9301 aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta ttgaagcgtt
     9361 agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg taacggaatc
     9421 aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa cgaattgtgc
     9481 cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta cgcatctagt
     9541 cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac aagtaatggc
     9601 agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg tgacgaaggc
     9661 aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat aactcatgtt
     9721 cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt tacagaaatg
     9781 aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt atttttaggt
     9841 gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc agacacaaca
     9901 actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa ccagtcagct
     9961 tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa tgatggcttc
    10021 caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca agcattgaat
    10081 gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat ccaagaacaa
    10141 gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga tggattagat
    10201 aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg tgttgcagca
    10261 gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc
    10321 gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga attaggaaaa
    10381 gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa aggacgttca
    10441 attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc aggcggtaca
    10501 ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg
    10561 gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa cggcgaaaag
    10621 aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga agatttggca
    10681 caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg attgaaagaa
    10741 gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc agttgatgaa
    10801 gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga agaaaactaa
    10861 aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg attagaacaa
    10921 gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca actccctgaa
    10981 gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca atcgttgccc
    11041 tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat
    11101 gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca atttacaagt
    11161 aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat ctacattgat
    11221 gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc gtgggaaaga
    11281 agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg tccatcagca
    11341 ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa gcgagcctag
    11401 tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag tttctgaaca
    11461 gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg gaatggaaca
    11521 acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt ttgatgagcc
    11581 gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga aggaattagt
    11641 caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca aagcagtagc
    11701 tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt
    11761 aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca aaacgatgaa
    11821 aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa
    11881 tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg
    11941 tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga ctggtttgcg
    12001 aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata aaaaaccccg
    12061 aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat acgggttagt
    12121 cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa aaccttacag
    12181 taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat tgatcgaaga
    12241 atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag gcggaaatca
    12301 gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa
    12361 tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc tgatcgaaca
    12421 aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt
    12481 ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg atccgaaaga
    12541 aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag aagcacgaaa
    12601 agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc cgtaatttat
    12661 tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg atggctgctt
    12721 ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt ggttcgcaaa
    12781 gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca ttaggttttt
    12841 ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca ctttgcgggt
    12901 ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt gttttgcttc
    12961 cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt ccaggtttac
    13021 ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac tatatcttat
    13081 tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc tcaagtttgg
    13141 actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta acacaagctt
    13201 ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt attttcgttt
    13261 ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga ttgaatcctt
    13321 ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg atggtcattt
    13381 ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct
    13441 ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc ttgcttggtt
    13501 ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg aagatcggtg
    13561 gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt
    13621 tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct aaagtcttcc
    13681 aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg aatcaaaacc
    13741 atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc ttacttctac
    13801 actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt ctgaacgaag
    13861 cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta gttccatcgt
    13921 tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg ctattttagt
    13981 gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag tcagtttgcg
    14041 tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt taggtgtttt
    14101 cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga atctggggta
    14161 tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct ttaaaaatac
    14221 attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga tcatcttcaa
    14281 gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg ccgacacatt
    14341 aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct tgttaggcgg
    14401 tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt
    14461 tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc ctttaggggc
    14521 aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg gcgcacaatt
    14581 accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt tgctgacaat
    14641 cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg ggaagaacta
    14701 tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg
    14761 tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat aatcatgttt
    14821 aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc agatgccgat
    14881 aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc aggattgaac
    14941 gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg tgtggaatca
    15001 gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg
    15061 atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg tgtgtttcct
    15121 aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg taaagtcgta
    15181 tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca ccaaatgaaa
    15241 acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa
    15301 gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga tataacaaaa
    15361 gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga accaggagac
    15421 ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca cacagtcctt
    15481 gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa cgatattttc
    15541 aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg cgttgaccag
    15601 ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt agtagatttt
    15661 gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat tgaagaattt
    15721 gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct aatgattaca
    15781 gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga atatgtaccg
    15841 ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg atattattca
    15901 gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc
    15961 ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc aagttaagcg
    16021 agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa attgattttg
    16081 gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat gctgtaatga
    16141 tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat gcaggaaagc
    16201 tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga ttccactact
    16261 atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc ggactagggg
    16321 cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag
    16381 atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg atcggtccga
    16441 atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg cctgactatc
    16501 aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa ggcgtatata
    16561 tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct cggacaatag
    16621 gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct cacagcggat
    16681 taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa gcgaatctga
    16741 gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa gcattggtta
    16801 aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca tattgaagcg
    16861 aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg agctaaatat
    16921 attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg tatgttaggc
    16981 tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat gggaatgcca
    17041 tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa attgattcga
    17101 gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt actagcacaa
    17161 ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca ttttcctcca
    17221 atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa gggctatacg
    17281 gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt gacagagacg
    17341 ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc tctttattac
    17401 ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga ccatttgatc
    17461 accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat gatcgaagta
    17521 ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct gcattaagta
    17581 ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga cttattattg
    17641 caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt ttaactctga
    17701 gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga
    17761 ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac aaatcgtgtc
    17821 acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg ttaagtcatc
    17881 atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga tgtcttcgtc
    17941 agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg cagctactaa
    18001 aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg gcaaagcgcg
    18061 ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta acattgcgta
    18121 acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag gaagagtatc
    18181 gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg tttctttttt
    18241 gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt tgatttgtgg
    18301 tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg cacgagttaa
    18361 aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc gtccgccttc
    18421 ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag taaattggtt
    18481 caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca tgtttagctc
    18541 tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg tacaattttt
    18601 tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag tagagaatat
    18661 aaaaaaacga acagtgccca cgcaactgtt cgtttttagg tattagatgc ataattgctc
    18721 gatttctttt tcaggtaaat tgatatattt ataacatgtt ccaactgaaa caccgcattt
    18781 tgaagaaata aattggatcg tttctttttt ttcataatag agacgtcgga tttttttgat
    18841 cgtttttgca tcgatttttg gtcttccgcc gatttttcct tttttacgag cttcatctaa
    18901 tccaactaat gtacgttctt tgatcaatgc gcactccatg tttgctaggt tttccattaa
    18961 ttggaaatag atttttccca tttcgcgcgt atcgattcct tcatcaagac taatcaagtg
    19021 cagcccgcga tctttgaata attgcgtgag ttctgttaat tgccgcgtag atttgcctaa
    19081 tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc atgtcagcga tcactgactc
    19141 caaatgaaca gcagtagaat cattttgttc cacttcatat gtctcatgga aaatttcttg
    19201 acaaccaaag tcagataatt gatcaacctg agttgttaaa tcaacgtcat ttatggttgt
    19261 gcgtgcataa ccaataattt tcatggtgat tcctttctag cagtgatttt ctaactggat
    19321 ataacagtaa aaatgtagca taaagtacgt caaaaagata gttttttcgc aaattttccg
    19381 ctgtcttttt ttattggatg atcaagcagt ttttctttat ttacgacgga ttgcgtcaag
    19441 aatctcaacg cctttttctg agccgacaac tactttagaa accatgttta ggaaaagccc
    19501 atgttctact accccaacaa gttcatctaa ataggtagct aagctttctg gctgttcgat
    19561 gacttctaga tgcagatcaa tgatgtaatg accaccatct gtggttaaga cttctccgtt
    19621 ttcgtctgta cgcaagacgg gttggaatct tttttcttca aaaatatgaa gtagctgttg
    19681 ggagccataa gggatcactt caactgggag tgggaacttc ccaagtttat cgactaattt
    19741 agaagaatca acgatccaaa tcgtttcttt tgagtacgta gcgactattt tctcgaaaag
    19801 caaagcagcg ccgccgcctt tgatcccttg aaaatcagca ctgatttcat cagcaccgtc
    19861 tattgtcaaa tcgactacgg ggacttcatc aatactcttt aacggtattc ctaatttttc
    19921 cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac cttcttcacg
    19981 catacggcga ccaatctctt ctaccatata ataggcggtt gaaccggtcc cgagacctac
    20041 gatcatcccg tcctttacga atttcgcggc ttcaatgcca gctaattgtt ttaaattcat
    20101 ctttgcgtcc accttttttt agtttgagca tcgtgctttc ctcactattt tagcgtgatt
    20161 tacggtaaaa agataccatt cttttttatt ccaataaagc tttttcaaaa aaaattgcat
    20221 ctcttgttga catgtttttt cattcgtgat attctaacaa aggtgcttta tctacaggtt
    20281 tctgtgaaga aacgtgctga ctgtttatca aagcatattt gataaggatg cagacattgc
    20341 atttattttt tatcacaaaa aaagaaccac ctggatgtgt ggaactagac gcaaatcgtg
    20401 ggaaggagga aacaaggaat gcctacaatt aaccaattag tacgtaaacc tcgtaaatca
    20461 aaggtggaaa aatctaattc acctgctttg aacaaaggat ataacagctt taagaaaact
    20521 caaactaacg taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg tacaatgaca
    20581 cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc aaacttaatt
    20641 gaagttactg cttatatccc agggattggt cataacttac aagaacacag tgttgtatta
    20701 ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt accatatcgt tcgcggtgct
    20761 ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg aactaaaaaa
    20821 cctaaagctt aattttaatt attaaacaaa tgaaacttgc ttctactata tagaagtaat
    20881 tactatgaac aatatttcgg aaggaggagt tacggatgcc acgtaaaggt cctgttgcaa
    20941 aacgtgatgt tttaccagat cctatttata actcaaaatt agtaactcgc ttgatcaacc
    21001 gtgtaatggt tgacggaaaa cgcgggattg cagctaacat tatctataat gcctttgact
    21061 tgatcaaaga atctacaggt aacgatccat tagaagtgtt tgaacaagca atgaaaaacg
    21121 ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac caagtaccag
    21181 ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg ttgggtagta aactacgctc
    21241 gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa agaaatcatg gatgctgcaa
    21301 acaacacagg tgcttctgtt aaaaaacgtg aagacactca taaaatggct gatgccaacc
    21361 gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt ggtcggcttt tataagtcga
    21421 cctcagatag tgtccttatt tttagcaact agatagtaaa gagaggagaa acaaggagaa
    21481 tgaccagaga attttcttta caaaacactc gtaatatcgg tatcatggcc cacatcgatg
    21541 ctggtaaaac gactgctact gaacgtatct tgtactatac tggtaaaatc cataaaattg
    21601 gtgaaactca tgaaggagct tcacaaatgg actggatgga acaagaacaa gaacgtggta
    21661 ttacgatcac ttctgctgct actacagcac aatggaaggg tcaccgaatc aacatcatcg
    21721 acacaccagg acacgtagac ttcacagttg aagttgaacg ttcattgcgt gtacttgatg
    21781 gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa acagtttggc
    21841 gtcaagcaac aacttatggt gtacctcgta tcgtatttgc aaacaaaatg gacaagatcg
    21901 gtgctgactt cttatattca gtatcaactt tacatgaccg tttgcaagca aacgctcacc
    21961 caatccaatt accaattggt gcggaagacg acttcacagg aattatcgac cttgtaacaa
    22021 tgaaggctga aatgtataca aacgatcttg gaacagagat cgaagaaaca gaaattcctg
    22081 aagaataccg cgaattagct gaagaatggc gcgaaaaatt ggttgaagct gtagctgaaa
    22141 ctgatgaaga acttactttg aaatacttag aaggtgaaga aatcactgaa gctgagttaa
    22201 aagaaggtat ccgccgcgca acagttaacg tagaattcta cccagtacta tgtggttcag
    22261 catttaagaa caaaggggtt caattactat tggacgctgt tcttgattac ttgccatcac
    22321 cgttagatat cccagctatc aaagggattg accctaaaac tgatgaagaa gtagaacgtc
    22381 ctgcagacga cagcgctcct ttctcagctt tagcatttaa agttatgact gacccattcg
    22441 taggtcgttt aactttcttc cgtgtgtact ctggtgtttt acaatcaggt tcatacgtac
    22501 aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg catgccaact
    22561 ctcgttcaga aatctctgaa gtttacgctg gagatatcgc tgctgctgtt ggtttgaaag
    22621 acacaactac tggggacaca ctatgtgatg aaaagagtct agttattctt gaatccatgg
    22681 aattcccaga accagttatc caagtcgcta ttgaacctaa atcaaaagct gaccaagata
    22741 aaatgggtgt ggctttacaa aaactatctg aagaagatcc tactttccgt gctgaaacaa
    22801 acgtggaaac aggcgaaaca atcatcgctg gtatgggtga acttcactta gatatcatcg
    22861 ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct caagtatctt
    22921 accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt cagtctggtg
    22981 gtaaaggtca atacggtcac gtatggattg aatttacacc aaatgaagaa ggtgctggtt
    23041 tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt ccagctgttg
    23101 aaactggttt gaaagatgct atggaaaatg gtgtcttagc tggctatcca ttagtagata
    23161 tcaaagctaa actttacgat ggttcatacc atgatgtcga ttcaaatgaa actgccttcc
    23221 gtgttgctgc ttctatggca ttacgtgcag cagctaagaa agctaatcca gtaatccttg
    23281 aaccaatcat ggctgtagaa gttgttatcc ctgaagatta tttaggtgat gttatgggtc
    23341 acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc ggccaacaag
    23401 ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta tgctacaact cttcgttctg
    23461 ctactcaagg tcgtggtaca tttacaatga catttgacca ctacgaagat gtaccaaaat
    23521 cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc tgaataattt cttgaaaccg
    23581 gatagtcctg caaagagatt atccaaagag actatatttt taatatagtt tcgtgtaaaa
    23641 taattatcga tgatatgggc atgtggtgta ccttgtgtac atcatatacc tatcagacaa
    23701 ataaaaaata tattgtattt ttaggaggac attttaaaat ggcaaaagaa aaatttgacc
    23761 gttctaaacc acacgttaac attggtacta tcggacacgt tgaccatggt aaaactacat
    23821 taacagctgc aatcacaact gtactatcta agaaaaacgg cggccaagct atggcttacg
    23881 atcaaatcga tggtgctcca gaagaacgcg aacgcggaat cacaatctca acagctcacg
    23941 ttgagtatga aacagataca cgccactacg ctcacgttga ctgcccagga cacgcggatt
    24001 acgttaaaaa catgatcact ggtgctgctc aaatggacgg agctatcttg gtagtttctg
    24061 ctgctgacgg cccaatgcct caaactcgtg aacacatcct attgtctcgt caagttggtg
    24121 ttccttacat cgttgtattc ttgaacaaag tagacatggt tgatgacgaa gaattactag
    24181 aattagttga aatggaagtt cgtgacctat taacagaata cgaattccct ggtgacgatg
    24241 ttcctgtagt tgctggatca gctttgaaag ctctagaagg cgacgcttca tacgaagaaa
    24301 aaattcttga attaatggct gcagttgacg aatacatccc aactccagaa cgtgacaacg
    24361 acaaaccatt catgatgcca gttgaagacg tgttctcaat tactggacgt ggtactgttg
    24421 ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa gttgttggta
    24481 ttgctgaaga aacttcaaaa acaacagtta ctggtgttga aatgttccgt aaattgttag
    24541 actacgctga agctggagac aacattggtg ctttactacg tggtgttgca cgtgaagaca
    24601 tccaacgtgg acaagtttta gctaaaccag gtacaatcac acctcataca aaattctctg
    24661 cagaagtata cgtgttgaca aaagaagaag gtggacgtca tactccattc ttcactaact
    24721 accgtccaca attctacttc cgtacaactg acgtaacagg tgttgttgaa ttaccagaag
    24781 gaactgaaat ggttatgcct ggcgacaacg taacaatgga agttgaatta atccacccaa
    24841 tcgctatcga aaacggtact aaattctcaa tccgtgaagg cggacgtaca gttggtgccg
    24901 gtgtcgttac agaaatcaaa gcttaattca cttaagtttt aaagtaaagc aacatttatt
    24961 cggacgtaat cgctcggaac gaaggtctta ggatctttgt tccgagcttt tttatttatt
    25021 gcaggatcag gaaaaaagac aggagtatta taaatagaag aaacacgaaa taaaagagtc
    25081 agtattttct aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa aagccttgct
    25141 ttgtgagttt atatatatta taatgacaaa ggtgcgttta ctttagtgaa ttgcacagac
    25201 gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc ggacgctttg
    25261 ccacgaacga gcgtgacgga aattttcgtg gagctatgtc tacttttcaa aataggcgaa
    25321 ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta tgaacaccgt
    25381 attttagatc aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg agctagcgtg
    25441 tctggtccaa ttccattacc aactgaacgc agtctttaca caatcatccg tgcgactcat
    25501 aaatacaaag attcacgcga acaattcgaa atgcgtacac acaaacgtct tatcgacatt
    25561 gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc tagacttacc atctggtgta
    25621 aacatcgaaa tcaaattata aaactaaaac aaaaataatg gaggtgtact catgaccaaa
    25681 ggaatcttag ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg tgaattaatt
    25741 ccagtaactg ttgtagaagc tactccaaac gtagtactac aagtgaaaac aatggaaacg
    25801 gatggctacg aagctattca agttggttac caagatatgc gtgaagtttt atcaaacaaa
    25861 cctgcgaaag gtcatgttgc aaaagcaaac acggctccta agcgcttcat tcgtgaattc
    25921 aaaaatgttg agctaggaga atatgaagta ggaaaagaaa ttacagtaga tgttttccaa
    25981 gcaggagaca ttattgatgt aacaggtact acgaaaggta aaggattcca aggggttatc
    26041 aaacgccacg gacaaagccg cggaccaatg gctcacggtt ctcgttacca tcgtcgtcct
    26101 gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa acaaacgtct tgctggccgt
    26161 atgggtggca accgcgtaac tatccaaaac ttggaagttg tacgtgtcga tgcagaaaga
    26221 aatgttatct tgatcaaagg taatgttcct ggagctaaaa aatctttaat tactatcaaa
    26281 tcagctgtga aagctaaata attgtaggaa gaaaggagga actaaggaat gccgaatgta
    26341 gcattattca aacaagatgg aagccaaaac ggtgaaatca cacttaacga agaaatcttc
    26401 ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg tgcgtcatta
    26461 agacaaggaa cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg acgtaaacca
    26521 tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta tccgctcacc acaatggcgt
    26581 ggaggtggcg tagtctttgg accaacacca cgttcttaca gctacaaact tcctaaaaaa
    26641 gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa caacttggta
    26701 gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag aattcaaaca agttcttgct
    26761 aacttatcta tcgactctaa agtattggtt gttctagaaa aaggtaacga ctttgctgct
    26821 ttatcagcac gtaacttacc aaacgtttct gtagtaactt ctgataatgt gagtgtgcta
    26881 gacgttgtat caaatacaaa agtgttagca acacaaactg ctcttactca aattgaggag
    26941 gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc agtgatcact gaaaaatcaa
    27001 tgcttgccat ggatgacaag aaatacactt ttgaagtgga cactcgcgca aacaaaactt
    27061 tagtaaaaca agcggtagaa gctgcttttg acgttaaagt caaaaacgtg aacatcgtga
    27121 acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg atatacaaaa aaacgtcgca
    27181 aagcgatcgt tacattgact gaagattcaa aagaaatcca attattcgaa gctgctgaat
    27241 aagcactaat agataactca ttaaattagg agggaaaaac gtggcgatta aaaagtacaa
    27301 acctacctca aatggtcgtc gtaacatgac aggttctgat tttgctgaaa tcacaacatc
    27361 aacacctgaa aaatcattgt tacaaccatt gaaaaaccat gctggacgta acaacaacgg
    27421 ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg tgatcgactt
    27481 caaacgtaac aaagataacg ttgttgcaac tgttcaaacg atcgaatatg atccaaaccg
    27541 ttcagctaac atcgcgttag tacactatga agatggagtg aaagcttaca tcttagcacc
    27601 taaaggacta gaagtgggta tgaaacttgt ttcaggtcca gaagcagata tcaaagtcgg
    27661 aaatgctctt ccattagaaa acattccagt tggtactgtt atccacaaca ttgaaatgaa
    27721 acctggtaaa ggtggacaat tgatccgttc tgctggtaca agtgctcaag tacttggtaa
    27781 agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa gttcgtatga tcttagctgc
    27841 atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac gaattaatca acatcggtaa
    27901 agctggacgc tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg taatgaaccc
    27961 taatgatcac ccacacggtg gtggtgaagg taaagctcct atcggacgta aagcaccagt
    28021 ttctccttgg ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag ctaaatcaga
    28081 caaacttatt gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg tcgcacattt
    28141 tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg atgagcattt
    28201 aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa aaagttatca aaacttggtc
    28261 acgccgttca acagttttcc caagttttgt tggatatacc atcgcagttt atgatggacg
    28321 taaacatgta ccagtttaca tccaagaaga catggtagga cacaaattag gtgaatttgc
    28381 acctacaaga acttatcgtg gacatgctgc tgacgataag aaaacgaaac gctaatttga
    28441 ggggaggata aatccaaatg gcagaacaaa tcacatcagc taaagcaact gcaaaaacag
    28501 ttcgcacttc acctcgaaaa gcccgtttag taattgacct tatcagaggt aaaagtgtcg
    28561 cagatgcaat ttcaatcttg aaattcacac caaacaaatc tgctggaatc attgaaaaag
    28621 tattgatgtc agcagttgcg aatgcagaaa ataactttga cttagatgtt gaaaacttgg
    28681 tagtaagcga agcttttgtt aacgaaggac ctacaatgaa acgtttccgt cctcgtgcaa
    28741 aaggatcagc ttctccaatc aacaaacgta caagtcatat cacggtagtc gtatcagaaa
    28801 aataaggagg gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg tgtaggcatc
    28861 atccgcgact gggatgctaa atggtatgct gaaaaagagt atgctgagtt cttacacgaa
    28921 gatttaagaa tccgtaaatt tatttcaact aaacttgctg atgctgctgt atcaacagtt
    28981 gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc acacagctaa accaggtatg
    29041 gttattggta aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa caaattaact
    29101 ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac cagatctaga tgcaaaatta
    29161 gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg cgctcaaaaa
    29221 caagccatcc aacgctcaat gcgtgctggc gctcaaggga tcaaaactca agtatctggt
    29281 cgtctaaacg gagcagatat cgctcgttca gaaggatact cagaaggaac tgttcctctt
    29341 catactttac gtgcggacat tgattacgca tgggaagaag cagatacaac atacggaaaa
    29401 ctaggagtta aagtgtggat ctatcgtgga gaaattcttc caactaaaaa aaacactgag
    29461 aaaggaggga aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt gaattccgcg
    29521 gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc attcggtgaa tacggtttgc
    29581 aagctgttga ttcacattgg atcacaaacc gccaaatcga agctgctcgt atcgcaatga
    29641 ctcgttacat gaaacgtggt gggaaagtat ggattaaaat tttccctcac aaatcttata
    29701 ctgccaaagc aattggggta cgtatgggtt ctggtaaagg ggcacctgaa ggatgggttg
    29761 caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa gaagtagctc
    29821 gtgaagcgtt acgtctagct tctcacaaat taccaatgaa aactaagatc gtaaaacgtg
    29881 aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca gagaattaac cactgccgaa
    29941 atgcttgatc aagaaaaaca attaaaagaa gaattgttta atcttagatt ccaattagca
    30001 acaggtcaat tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat tgcacgcatc
    30061 aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg aggccattaa gcgtatgact
    30121 gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa aatggataaa
    30181 acaattaccg ttgctgtcga aacaaagaaa aaccacccta tctatggcaa acgcatgaac
    30241 tattctaaga aatacaaagt gcatgatgaa aacaacacag caaaagttgg cgacatcgtg
    30301 agaattatgg aaactcgtcc attatcagct acaaaacgtt tccgtttgtt ggaagtagtc
    30361 gaagaagcag ttattatcta ataacacgag attttcctat atagattgaa tgcaaaatct
    30421 gaaaggagga tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc tgataactca
    30481 ggcgcacgtg aaattttgac gatcaaagtc cttggtggat ctggtcgtaa aactgctaac
    30541 atcggtgatg tgatcgttgc tacggttaaa caagcaacgc caggtggggt tgtcaaaaaa
    30601 ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg tacagacggt
    30661 tcatatatta aatttgatga aaatgctgca gtgattattc gtgatgataa aagccctcgt
    30721 ggaacacgta tctttgggcc agttgcacgt gaactacgtg aaaacaactt catgaagatc
    30781 gtttcactag caccagaagt tttataatct tgagacactt atcaaaggag gtgcgaaaca
    30841 gttatgtttg ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga caaaaacaaa
    30901 gaaggcgttg tactagcagc gttcccgaaa aaagataaag ttattgtcga aggtgtaaat
    30961 gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc aaggtggaat tgttgagatg
    31021 gaagcgccaa ttcatgtttc taacgtgatg gtggttgact ctactggtgt agctggccga
    31081 gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac cggtgaagtt
    31141 ttagataaat aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct aaaagaaaaa
    31201 tatcttaaag aagtaactcc atcattgatg gaaaaattta actatagctc tgttatgcaa
    31261 acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt atcaaacgct
    31321 aaaaacttag ataaagcagt tgaagaatta actttgatca ctggtcaaaa accaatgatt
    31381 acaaaagcta aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc aattggtgca
    31441 aaagttacat tgcgcggaga aagaatgtac gaattcttag acaaattggt ttctgtttca
    31501 ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag cctttgacgg tcgtggaaac
    31561 tatactttag gtattaaaga acaattgatc ttcccagaag tagattatga tttagtagat
    31621 aaagtacgtg gtatggacat cgttattgta acaacagcaa actctgatga agaatctcgt
    31681 gagttgttga cacaattagg catgccattc caaaaataat taaaggaggc gaactacgtg
    31741 gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa aattttcaac acaagcttat
    31801 actcgttgcg aacgttgcgg acgtccacat tcagtttatc gtaagttcca tctttgccgt
    31861 atttgcttcc gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa agctagctgg
    31921 taaaaagtaa attcgcataa gaaggaggta aaagatcaat ggtcatgaca gatccaattg
    31981 cagattttct aactcgtatc cgtaacgcaa acatggttaa acatgagtct ttagaagtac
    32041 ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt ttcgttcgtg
    32101 atgttgaata catcgaagat gacaaacaag gcgtgatccg tgttttccta aaatttggta
    32161 aaaacggcga acgtgttatc acaaacttaa aacgtatctc taaaccaggt ttgcgtgctt
    32221 atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt aggtatcgca atcatttcga
    32281 cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc ggcgaagtta
    32341 tcgcttacgt atggtaatca agtagaatct acaaggaggt gtctttaagt gagccgtatc
    32401 ggaaataaag tcgttgttat ccctgaagga gtaacagtta ctcaagacgg aaacaacgtt
    32461 acagttaaag gaccaaaagg tgaattgact cgtacttttt cagcagatat caaaatgaat
    32521 atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat gaaaacaatt
    32581 catggaacaa ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga aggtttccaa
    32641 aaagcgttag aactaatcgg ggttgggtac cgtgcacaat tacaaggatc aaaacttgta
    32701 ttgaacgttg gttactcaca tccagtagaa atcacaccac cagctggagt aactgttgaa
    32761 gtaccagcta acacacaagt aattgttaaa ggtgcgaaca aagaagaagt tggcgaatta
    32821 gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg tattcgttat
    32881 gttggcgaat atgtacgccg taaagaaggt aaaactggta aataatcgtt tagaaaaatt
    32941 gcatagaagc cagcagcagg aaaaaggcat ttggctgaag tttaccttca aaaaaataca
    33001 tttttacttg tgccgcagct tctatttttc gaaacgtatt tactgcttga aatagtagta
    33061 caataaaaat aaagaggtga caattgtgat tacaaaacca gataagaaca aaacacgtca
    33121 aaagagacat cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc cacgcttgaa
    33181 cgtttttcgt tctaacaaaa acatctacgc tcaaattatt gatgacgtag cgggtgtaac
    33241 gctagcaagt gcctctacct tggataaaga aatttcaggt ggaacaaaaa cagaagcagc
    33301 acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta aagtagtagt
    33361 ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg aagctgctcg
    33421 cgaaaatgga ctagaatttt agaagaagga ggaataccat tcatggttta tatcgatcca
    33481 aaacatttgg aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa agttgttaaa
    33541 ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa cggacacgta
    33601 ggatttggta caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc aattgaagat
    33661 gcgaagaaaa acttagttga agtacctatg gttggttcaa caatcccaca cgaagtaatc
    33721 ggtgctttta gtggtggtag aatcctaatg aaacctgctg tagaaggttc tggggttgct
    33781 gctggtggac cagttcgtgc cgtattagaa ttagctgggg tagcagatat cacttctaaa
    33841 tctttaggct caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg tttgaaacaa
    33901 ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat cagttgaaga attaatcgga
    33961 taaggaggac aacaataatg gctgaattaa aagttacttt aaaacgcagt attatcggac
    34021 gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct tacaaagatt aacagttcag
    34081 tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa tactgtttct cacctagtgg
    34141 acgttgaaga aatttaattt agaaaaatca taactagaac attaaggagg tgccaaacct
    34201 atgaaacttc atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa ccgtgttggt
    34261 cgtggtactt catctggtaa cggaaaaact gccggacgcg gacaaaaagg acaaaaagct
    34321 cgttcaggcg gcggtgtgcg tttaggattt gaagggggac aaacaccatt gttccgtcgt
    34381 ttaccaaaac gaggattcac aaacgtgaac cgtaaagact atgcagtcat taatcttgat
    34441 gtattgaacc gctttgaaga cggagctgaa gtaactcctg ttgctttagt tgaagctgga
    34501 atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta atggggaact aaacaagaaa
    34561 ttaactgtga aagcagcaaa attctctaaa gcagctcaag aagcaattga agcggctggt
    34621 ggatcaatcg aggtgatcta atgtttagac tattaaaaga tgcttttaaa gtaaaagaca
    34681 ttagatcgaa aatcctattt actgtatttg tgttgtttgt atttcgccta ggtgcacata
    34741 ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg ccatttttca
    34801 acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt ttccattttc tcaatggggg
    34861 tttcaccata cattactgct tctatcgtca ttcagcttct tcaaatggac atcgtgcctc
    34921 gatttgttga atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat caagcgactc
    34981 gatatctgac attagtattg gcatttatcc aatctgtcgg tatcactgct ggattcaatc
    35041 aatacgctca attaggtttc gtgaataatc ctaacgtagc aacttacgta acgatcggtt
    35101 tgattctaac agccggtacg atgtttgtta cttggctagg agaacagatc acagaaaaag
    35161 gtatcggaaa tggcgtatca atgatcattt ttgcaggtat catttctaga ttgcctgaaa
    35221 gtatcagaga aatttatgaa gattacttta taaatattga tccttcagac atatggaaat
    35281 ctgctatttt tgtggccatt ttactagtag cggtcttgtt gatcgtgaca ttcgtaacat
    35341 ttttccaaca agctgaacgc aagatcccga tccaatatac gaaacgcgta gctggcgcgc
    35401 ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg agttattcca gttatctttg
    35461 ctagttcgtt tattgctacg ccaaatgctg ttttacaagc attgagcggt agattcggtt
    35521 ctgaaaactg gtacaaagtg atgatggaga tctttaacta taatacagca cctggtgcta
    35581 cgatttatac gattctgatc gttgccttca ctttcttcta cgcctttgtt caagtaaacc
    35641 ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta tataccaagc gtacgccctg
    35701 gtaaggggac tgaagagtat gtctcaagat tactgatgcg tttaagtaca gttggtgcag
    35761 tcttccttgg tttagtggca ttgcttccaa tcattgctca aatggtttgg gatcttccac
    35821 aatcaatcgg attaggcggt acaagcttac tgatcgttat cggtgtagct ttagaaactg
    35881 cgaaacagtt agaaggctta atgctgaaac gtaaatatgt tggattcatt gagtaagaaa
    35941 gcgtgttgag gtttcccctc aacacacgtt ctcacacatt tattaggagg gaaaacaatg
    36001 aacctcattt taatggggct gccaggcgca ggtaaaggaa cacaagctga aaaaatcatt
    36061 gatgcttatg gtattccgca catctcaaca ggagatatgt tccgcgcagc gatgcaaaac
    36121 gaaacagctc ttggcctgga agcaaagtct tatatggata aaggtgcatt agttcctgat
    36181 gaagtaacaa atggaatcgt gaaagagcga ctagctgaac cagatacaga gaaaggcttc
    36241 ttattagatg gctttccacg tacgctagat caagcaaaag ctctggacgc aatgctaaaa
    36301 gatttgaata aaaaaattga tgctgtcatc gatatccatg ttggtgaaga aatcttggtc
    36361 gaacgtctag ccggccgatt tatctgcagc aattgtggag caacttacca taaaattttc
    36421 aaccctacaa aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt ctatcaaaga
    36481 gaagatgata aacctgagac ggttaaaaat cgtctggcta tcaacatcaa aaatagtgaa
    36541 ccaatcttgg cttattataa agaacaaggt ttgctaaaca ctattgatgg tgctcgtgaa
    36601 atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa aatagcacga ttgcccgtag
    36661 ccgacttgca ttatgcagtt actcttgtca agttcaataa attatgatag aatatgtcag
    36721 tccgacttct agagaatcct ctagatgatc cattttgaga attttgaggt gggaactgca
    36781 gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac tgtgcgtggc
    36841 aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa actttgccga atgcaatgtt
    36901 taaagttgaa ctagaaaacg gacaccaagt tcttgctact gtttcaggta aaattcgtat
    36961 gcattacatc cgtatcttac ctggtgacaa agtaacggtt gaattgtctc catacgatct
    37021 taaccgtggc cgtattactt atcgctttaa ataattgtac tccgtaaaac tacaggaggt
    37081 attatcatga aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa agtaattcgt
    37141 cgtaaaggac gcgttatggt gatttgccca gcaaatccaa aacataaaca acgtcaagga
    37201 taattggagg tgctataaat atggctcgta ttgcaggagt agatattcct cgtgataaac
    37261 gtgtagtagt ttcacttact tatatctatg gtattggtaa cactactgct aaaaagatct
    37321 tagctgatgc tggtgtatct gaagatgttc gcgttcgtga attaacgaat gaacaaacag
    37381 atgctatccg tgcagaagta gataaattaa aagttgaagg ggatcttcgt cgtgaagtga
    37441 acttgaacat caaacgtttg atggaaatcg gttcttaccg tggaatccgt catcgccgtg
    37501 gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa ggcccagctc
    37561 gcgctatcgc tggcaagaaa aaataattct taagtgaagg aggttaaaac ttcatggcag
    37621 caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt attgcacata
    37681 tccattctac attcaataac actatcatca tgatcactga tactcacgga aatgcattag
    37741 cgtggtcatc agctggttca ttaggtttca aaggaagcaa aaaatcaact ccttttgccg
    37801 ctcaaatggc agcagaaact gctacaaaag cagcaatgga acacggatta aagactgttg
    37861 aagtaacagt taaaggacct ggttctggac gtgaagcagc aattcgttca ttacaagcta
    37921 caggtttaga agtgactgca attcgtgacg tgactccagt tcctcataat ggtgcccgcc
    37981 ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc agtaacgtca
    38041 ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa gaatgattga gttcgaaaaa
    38101 ccaagaatcg caaaaattga tgaagaaaaa gattatggca agttcatcgt tgaacctctt
    38161 gaaagagggt atgggactac tttagggaat tccctacgtc gtattttatt atcttctttg
    38221 ccaggtgctg ccatcactaa tattcaaatc gatggtgtac tgcatgaatt ctctaccatc
    38281 aaaggtgtta gagaagacgt cactcaaatc atcttgaaca ttaaaggctt agcgctgaaa
    38341 atgtatggcc aagaagaaaa aacccttgaa atcgatatta ccggacctgc tacagtaact
    38401 gctggcgata ttatcgttga tagtgatgta gaaattctta acaaagatat gtacatctgt
    38461 agtgtctctg aaggagctac gttccatgct cgcttaacag tgaaacctgg acgtggttat
    38521 gttcaagcag atgaaaataa aaaagaagat atgccaattg gcgttcttcc agtcgattca
    38581 atctacacac ctgttcgtcg tgtgaactat caagttgaga acacacgtgt tggacaccgt
    38641 gaagattttg ataaattgac gatggagatt tggacagatg gttcaattga accgttggaa
    38701 gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg atatttttgt gaacttaact
    38761 gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa agaaaaaatg
    38821 ttggaaatga cgatcgaaga gttagacttg tctgttcgct cttacaattg tttgaaacgt
    38881 gcaggaatca atactgtaca agaacttaca aataaatctg agcctgaaat gatcaaagtc
    38941 cgtaacttag gacgtaaatc attagaagaa gttaaagcaa aattgcacga cttaggttta
    39001 ggtttacgta aagaagatta aaacctttta cgaaggaggg aaaccaacgt gagttatcgt
    39061 aaactaggac gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac aactgattta
    39121 ttaatcaacg aacgtattgt gactactgaa gctcgtgcga aagaagttcg ctcaactgca
    39181 gaaaaaatga tcactttagg taaacgcgga gatttgcatg cacgtcgtca agctgcggct
    39241 ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt cattgaatca
    39301 gctttacaaa aactgttcaa cgatattgca cctcgttacg cagaacgtca aggcggatac
    39361 acacgcattt tgaagacaga accaagacgc ggtgatggtg caccaatggt tgtcttagaa
    39421 cttgtctaaa atcacactaa taacatcact ttatagatga ttcttttaga gtgttatgat
    39481 gttggaagat atttttccga gtctagctca gcgctgctcc tcaagcgaat atcgcttggg
    39541 gagtaagcac tcatcataaa gtgttttttt ataccttcaa ttacctgtca tgacagatat
    39601 gtttgaaggc ataaaaaaac attttaagcg agaaataatt gaaaattttg tctttttttg
    39661 atagtgttag taggaacttt aggatgaaga aggagtaatc atggagccaa ttattgaatt
    39721 agaaaagata aattataaat accagccaga tgatcttcgc cccgcattga aagatgtctc
    39781 ctttacaatt gataaaggtg agtggatcgc tattattgga cacaatggtt caggaaaatc
    39841 gacacttgcc aaaacgatca atggattgct tctgccagaa tcagggatcg tgaaagtggg
    39901 aaatcaaata ttggatgaag aaaatatttg gacgatccga cagatggttg gaatggtttt
    39961 tcaaaatcca gacaatcagt ttgttggttc gacagtagaa gatgacgttg cttttggtct
    40021 tgaaaatcaa gggattccca gagaagaaat gctagtgcgt gtgaaagatg cacttgagaa
    40081 agttagaatg gctgagtttg cttcacgcga gcctgctcgt ttatcaggag ggcaaaaaca
    40141 acgtgttgcc attgccggcg tggttgcctt acgacctgat atcattattt tagatgaagc
    40201 aaccagtatg ctagatccag aaggtcgtga agaagtgatc tctacgatca agaagatcaa
    40261 agaagaaagt caattgaccg ttatatctat cactcatgat attgatgaag cagctaatgc
    40321 caatcggata ctagtgatga gacaaggcga acttgtccgt gaaggtacac caaaagaaat
    40381 tttttctgct ggtcctgaac tgatcgactt aggtctagat ctaccatttc ctgaaaaact
    40441 gaagagcgca ctgaaagaac gtggggttga tgtgcccagt gaatatatga cagaagaaag
    40501 gatggtggat tggttatgga catccgtttt gaacaagtag acttcactta tcagccgaat
    40561 acaccatttg aacaaagagc cttatttgac atcaatatga cgatcaaaga aaacagttat
    40621 actgcattag tagggcatac cggaagcggg aaatcaacct tacttcagca tttgaatgcg
    40681 cttgttaagc caacaagcgg tacagtccat attggagaac gagatattca gccggatacg
    40741 gataataaga atttaaagcc catccgaaaa aaagttggca tcgtctttca gtttccagaa
    40801 gcacagctat tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa aaactttggt
    40861 gtcagcgaag aagaagcact agtcctagca aaagaaacat tagaacaagt tgggctggat
    40921 gaaagctatt tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg tagagtagca
    40981 atcgctggtg tgcttgccat gaggccggaa gtacttgtat tggatgaacc aacggcagga
    41041 cttgatcccc agggcagaaa agaaatgatg gagatgtttt ggcgtcttca taaggaacag
    41101 cagatcacta ttgtattagt tacgcattta atggatgatg tagccaattt tgcagattat
    41161 gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga ggtctttcaa
    41221 aacattgaat ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga gtttgcagaa
    41281 gaattaatgg ctaaagggat gaactttgct accttgcctt tgacagctga agaattagca
    41341 gacgctatcg tccattatgc tggaggaaat gcccatgatg aataaattga tatttggtcg
    41401 ttatatgcct ggagattcct tgatccatcg attggatccc cgagctaaat tattagccag
    41461 tttttatttt atcggaatca tttttttagc aaataattgg gaaacgtacc ttttgttggc
    41521 atttttcaca ttactttctg tcttcttgtc aaaagtagat ttgaaatttt ttatccgagg
    41581 cattcgccca ttgatctggc taatcctctt cactgtagct ttgcaggtct tgtttacaag
    41641 tggcggagaa acttattgga gttggggtgt tttccatatc actgaatttg gtgtgatgaa
    41701 cggcctcttt attttctgtc gattcgtgtt gatcattttt atgtcgactc tactcacgct
    41761 tactacaccg cctttggaat tatcggatgc gattgagtac ttgcttcgtc cattgaaagc
    41821 cgtacggttc ccagtccacg aaatttcatt gatgctgtcg attgctcttc gttttgtgcc
    41881 tacattgatg gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg gtgttgattt
    41941 tggtgaagga aacctagttc agaaaatgaa agcagtcatt cctttgctga ttcctttatt
    42001 tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct atggaagcaa gaggctacaa
    42061 aggtggggaa ggaagaacga aatatcgtgt attgcattgg cataatagag acacgatggt
    42121 tatcattgct tttgtattat taactgtgct actgatcttt ttaagaggat aaacgttcag
    42181 tgggccgcta tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc aagcagtgta
    42241 tgacaagaaa tattagggtc tgtggcagac taaggtcaca ggcctctttc ttttatctga
    42301 aaagaaagaa cgaaaaaatt ttatagaaaa atcattattc cagataacct tttaggaaca
    42361 aaggagagaa aaacatggta cgttacaaag caatcattgc gtacgacgga acaaatttta
    42421 atggattcca aaaacagcca aatgggcgta ctgttcaaga agaagtagaa aaaacattac
    42481 aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc gggaaggaca gatgccggag
    42541 tacatgcgat cgggcaagta atccattttg attatccaga ggagcgtccg ttagaacgaa
    42601 tgcgtttcgc gctagatacc cagtcaccag aggatattgc tgtaagacaa gtagaaattg
    42661 tttcagaaga cttccacgca agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg
    42721 atatagggaa accacgcagt ccttttcgac gccattatgc aagttatttt ccttacccac
    42781 ttgatttgga aaaaattcag cgggcgcttc ctgatttatt agggactcac gatttcacct
    42841 ctttttgtgc atcaggaagc tcgatagagg ataaaatacg tacgatctac gaggctaaga
    42901 tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt taggggaaat ggcttcttgt
    42961 ataagatgat acggatctta gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg
    43021 aagatagtat cccagatatt attgcgaaaa aagatcgtaa tgcagcagga cctacagcac
    43081 accctgaagg actttatttg tatgaagtga aatatgaata agaggttatg atagaagtgg
    43141 tcagcttcaa gaaataagaa ggtatttacg aaaattactt ctcaaaattt tgcaaaattt
    43201 cggcttattt tcgaagaagc tacttctgga acaccgttta aggaagaggt cgtgaaatca
    43261 gcgttttgac ctgagtatta gagaaacaaa cgggaaaata gctcctcata ttttgccgtt
    43321 tgttgagcta ataccgaagg tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc
    43381 tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca tctttgatga
    43441 atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta cctatttttt
    43501 ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac agtctgaaca
    43561 ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc
    43621 tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc agacataaag
    43681 aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa
    43741 gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga atgcgtcttt
    43801 gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt tgtcataagg
    43861 aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag cttttctgcc
    43921 agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt gataggctac
    43981 aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc cataaaaact
    44041 tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt attgaccaga
    44101 aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac tagaactttc
    44161 taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg caggcacatg
    44221 gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag tgacgccagt
    44281 cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat agaatgtaaa
    44341 atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg tcaaattatc
    44401 tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta gtggcaatgc
    44461 attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga agccatattt
    44521 actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc catccatcag
    44581 caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga aacctaattt
    44641 atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga taccactgta
    44701 accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc ggtaaaacga
    44761 gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag gatcagtata
    44821 taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac tgttcgtatt
    44881 gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat tgaatgaaag
    44941 ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa
    45001 tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc cccatgccac
    45061 cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccacgat cgtcttgttc
    45121 tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca gataggcata
    45181 acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca atggaacaag
    45241 ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat ttttcaacgg
    45301 tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac ctgcgtaaac
    45361 gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt tcatgattag
    45421 atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt
    45481 tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa
    45541 agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg tcaatagacg
    45601 ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa aactggataa
    45661 aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca taacacgatg
    45721 aatccccaaa atcactaatg gaagataaaa aaatgcatcc agccacatga tgatctccga
    45781 atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat gcccccagcg
    45841 aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc cgaccttaag
    45901 caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga acaccagtgg
    45961 cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc ctagagaagc
    46021 attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc tggcatgaaa
    46081 attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc caggatagat
    46141 tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac tagctgctgc
    46201 gtagcgtccg ttttctctaa agaatgactt taaa
//
LOCUS       DACDHJ010000005        42615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDHJ010000005 DACDHJ010000000
VERSION     DACDHJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.005"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            342..1031
                     /locus_tag="IV223_000222"
     CDS             342..1031
                     /locus_tag="IV223_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8710533.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1018..2226
                     /locus_tag="IV223_000223"
     CDS             1018..2226
                     /locus_tag="IV223_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8710534.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2520..3791
                     /gene="serS"
                     /locus_tag="IV223_000224"
     CDS             2520..3791
                     /gene="serS"
                     /locus_tag="IV223_000224"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8710535.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="IV223_000225"
     CDS             complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="IV223_000225"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8710536.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6329..7024)
                     /locus_tag="IV223_000226"
     CDS             complement(6329..7024)
                     /locus_tag="IV223_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8710537.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="IV223_000227"
     CDS             complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="IV223_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8710538.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8223..8414)
                     /locus_tag="IV223_000228"
     CDS             complement(8223..8414)
                     /locus_tag="IV223_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8710539.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8435..9325)
                     /locus_tag="IV223_000229"
     CDS             complement(8435..9325)
                     /locus_tag="IV223_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8710540.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9312..10079)
                     /locus_tag="IV223_000230"
     CDS             complement(9312..10079)
                     /locus_tag="IV223_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8710541.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="IV223_000231"
     CDS             complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="IV223_000231"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8710542.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="IV223_000232"
     CDS             complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="IV223_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8710543.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="IV223_000233"
     CDS             complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="IV223_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8710544.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14695..15033
                     /locus_tag="IV223_000234"
     CDS             14695..15033
                     /locus_tag="IV223_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710545.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15201..15959)
                     /locus_tag="IV223_000235"
     CDS             complement(15201..15959)
                     /locus_tag="IV223_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8710546.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15977..16795)
                     /locus_tag="IV223_000236"
     CDS             complement(15977..16795)
                     /locus_tag="IV223_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8710547.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="IV223_000237"
     CDS             complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="IV223_000237"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8710548.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="IV223_000238"
     CDS             complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="IV223_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8710549.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17923..19257
                     /gene="dnaA"
                     /locus_tag="IV223_000239"
     CDS             17923..19257
                     /gene="dnaA"
                     /locus_tag="IV223_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8710550.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19455..20585
                     /gene="dnaN"
                     /locus_tag="IV223_000240"
     CDS             19455..20585
                     /gene="dnaN"
                     /locus_tag="IV223_000240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8710551.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20814..21056
                     /gene="yaaA"
                     /locus_tag="IV223_000241"
     CDS             20814..21056
                     /gene="yaaA"
                     /locus_tag="IV223_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8710552.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21043..22167
                     /gene="recF"
                     /locus_tag="IV223_000242"
     CDS             21043..22167
                     /gene="recF"
                     /locus_tag="IV223_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8710553.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22164..24110
                     /gene="gyrB"
                     /locus_tag="IV223_000243"
     CDS             22164..24110
                     /gene="gyrB"
                     /locus_tag="IV223_000243"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8710554.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24130..26601
                     /gene="gyrA"
                     /locus_tag="IV223_000244"
     CDS             24130..26601
                     /gene="gyrA"
                     /locus_tag="IV223_000244"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8710555.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26837..27136
                     /locus_tag="IV223_000245"
     CDS             26837..27136
                     /locus_tag="IV223_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8710556.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27186..27698
                     /gene="ssb"
                     /locus_tag="IV223_000246"
     CDS             27186..27698
                     /gene="ssb"
                     /locus_tag="IV223_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8710557.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27724..27960
                     /gene="rpsR"
                     /locus_tag="IV223_000247"
     CDS             27724..27960
                     /gene="rpsR"
                     /locus_tag="IV223_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8710558.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28103..30076
                     /locus_tag="IV223_000248"
     CDS             28103..30076
                     /locus_tag="IV223_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8710559.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30082..30534
                     /gene="rplI"
                     /locus_tag="IV223_000249"
     CDS             30082..30534
                     /gene="rplI"
                     /locus_tag="IV223_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8710560.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30808..32175
                     /gene="dnaB"
                     /locus_tag="IV223_000250"
     CDS             30808..32175
                     /gene="dnaB"
                     /locus_tag="IV223_000250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8710561.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32955..34247
                     /locus_tag="IV223_000251"
     CDS             32955..34247
                     /locus_tag="IV223_000251"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8710562.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35034..35897
                     /locus_tag="IV223_000252"
     CDS             35034..35897
                     /locus_tag="IV223_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8710563.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36026..37648)
                     /locus_tag="IV223_000253"
     CDS             complement(36026..37648)
                     /locus_tag="IV223_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8710564.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37897..38418
                     /locus_tag="IV223_000254"
     CDS             37897..38418
                     /locus_tag="IV223_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710565.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38604..40334)
                     /gene="spxB"
                     /locus_tag="IV223_000255"
     CDS             complement(38604..40334)
                     /gene="spxB"
                     /locus_tag="IV223_000255"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8710566.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40499..41317
                     /locus_tag="IV223_000256"
     CDS             40499..41317
                     /locus_tag="IV223_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8710567.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41420..41617)
                     /locus_tag="IV223_000257"
     CDS             complement(41420..41617)
                     /locus_tag="IV223_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710568.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41786..42364
                     /locus_tag="IV223_000258"
     CDS             41786..42364
                     /locus_tag="IV223_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8710569.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        12947 a         8192 c         8435 g        13041 t
ORIGIN      
        1 gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa caaataataa
       61 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
      121 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      181 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      241 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      301 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      361 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      421 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      481 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      541 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      601 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      661 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      721 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      781 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      841 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      901 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      961 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
     1021 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1081 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1141 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1201 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1261 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1321 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1381 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1441 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1501 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1561 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1621 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1681 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1741 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1801 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1861 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1921 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1981 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     2041 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2101 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2161 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2221 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2281 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2341 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2401 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2461 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2521 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2581 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2641 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2701 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2761 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2821 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2881 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2941 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     3001 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3061 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3121 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3181 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3241 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3301 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3361 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3421 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3481 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3541 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3601 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3661 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3721 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3781 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3841 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3901 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3961 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     4021 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4081 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4141 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4201 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4261 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4321 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4381 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4441 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4501 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4561 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4621 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4681 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4741 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4801 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4861 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4921 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4981 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     5041 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5101 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5161 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5221 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5281 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5341 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5401 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5461 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5521 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5581 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5641 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5701 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5761 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5821 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5881 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5941 ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     6001 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6061 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6121 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6181 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6241 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6301 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6361 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6421 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6481 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6541 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6601 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6661 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6721 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6781 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6841 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6901 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     6961 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     7021 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7081 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7141 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7201 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7261 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7321 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7381 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7441 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7501 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7561 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7621 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7681 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7741 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7801 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7861 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7921 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     7981 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     8041 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8101 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8161 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8221 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8281 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8341 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8401 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8461 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8521 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8581 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8641 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8701 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8761 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8821 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8881 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     8941 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     9001 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9061 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9121 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9181 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9241 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9301 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9361 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9421 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9481 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9541 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9601 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9661 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9721 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9781 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9841 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9901 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
     9961 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
    10021 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10081 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10141 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10201 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10261 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10321 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10381 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10441 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10501 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10561 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10621 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10681 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10741 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10801 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10861 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10921 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    10981 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    11041 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11101 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11161 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11221 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11281 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11341 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11401 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11461 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11521 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11581 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11641 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11701 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11761 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11821 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11881 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    11941 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    12001 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12061 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12121 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12181 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12241 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12301 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12361 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12421 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12481 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12541 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12601 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12661 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12721 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12781 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12841 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12901 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    12961 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    13021 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13081 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13141 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13201 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13261 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13321 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13381 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13441 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13501 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13561 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13621 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13681 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13741 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13801 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13861 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13921 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    13981 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    14041 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14101 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14161 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14221 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14281 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14341 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14401 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14461 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14521 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14581 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14641 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14701 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14761 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14821 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14881 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    14941 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    15001 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15061 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15121 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15181 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15241 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15301 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15361 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15421 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15481 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15541 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15601 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15661 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15721 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15781 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15841 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15901 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    15961 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    16021 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16081 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16141 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16201 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16261 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16321 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16381 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16441 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16501 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16561 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16621 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16681 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16741 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16801 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16861 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16921 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    16981 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    17041 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17101 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17161 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17221 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17281 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17341 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17401 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17461 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17521 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17581 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17641 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17701 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17761 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17821 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17881 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    17941 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    18001 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18061 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18121 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18181 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18241 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18301 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18361 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18421 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18481 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18541 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18601 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18661 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18721 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18781 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18841 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18901 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    18961 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    19021 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19081 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19141 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19201 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19261 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19321 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19381 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19441 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19501 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19561 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19621 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19681 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19741 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19801 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19861 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19921 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    19981 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    20041 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20101 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20161 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20221 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20281 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20341 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20401 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20461 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20521 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20581 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    20641 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    20701 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
    20761 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
    20821 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
    20881 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
    20941 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
    21001 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
    21061 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
    21121 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
    21181 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
    21241 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
    21301 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
    21361 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
    21421 agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
    21481 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
    21541 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
    21601 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
    21661 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
    21721 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
    21781 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
    21841 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
    21901 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
    21961 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
    22021 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
    22081 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
    22141 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
    22201 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
    22261 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
    22321 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
    22381 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
    22441 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
    22501 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgtaaacg
    22561 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
    22621 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
    22681 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
    22741 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
    22801 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatca
    22861 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
    22921 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
    22981 attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga acacatgaat
    23041 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
    23101 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
    23161 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
    23221 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
    23281 aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc agagctcgtc
    23341 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
    23401 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
    23461 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
    23521 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
    23581 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
    23641 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
    23701 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
    23761 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
    23821 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
    23881 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
    23941 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
    24001 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
    24061 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
    24121 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
    24181 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
    24241 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
    24301 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
    24361 taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc acagccattc
    24421 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
    24481 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
    24541 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
    24601 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
    24661 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
    24721 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
    24781 tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta tgaaacaggt
    24841 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
    24901 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
    24961 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
    25021 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc cgttatcttg
    25081 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
    25141 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta tatcgaacat
    25201 caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga agcaagagca
    25261 cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
    25321 cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt cgatttatcc
    25381 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
    25441 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
    25501 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
    25561 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
    25621 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
    25681 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
    25741 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
    25801 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
    25861 gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc gagtgaaagc
    25921 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
    25981 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
    26041 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
    26101 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
    26161 gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct gcgtgaggaa
    26221 gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt gatcacagaa
    26281 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
    26341 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
    26401 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
    26461 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
    26521 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
    26581 gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa gaataaccga
    26641 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
    26701 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta gagcttgtga
    26761 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
    26821 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
    26881 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
    26941 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
    27001 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
    27061 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
    27121 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
    27181 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
    27241 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
    27301 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
    27361 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
    27421 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
    27481 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
    27541 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
    27601 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
    27661 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
    27721 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
    27781 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
    27841 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
    27901 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
    27961 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
    28021 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
    28081 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
    28141 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
    28201 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
    28261 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
    28321 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
    28381 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
    28441 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
    28501 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
    28561 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
    28621 tggtatttta tctattgata actatgcaga tgccattgat aaattagatg acaaagagat
    28681 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
    28741 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
    28801 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
    28861 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
    28921 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
    28981 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
    29041 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
    29101 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
    29161 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
    29221 tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
    29281 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
    29341 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
    29401 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    29461 tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    29521 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    29581 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    29641 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    29701 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    29761 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    29821 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    29881 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    29941 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    30001 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    30061 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    30121 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    30181 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    30241 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    30301 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    30361 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    30421 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    30481 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    30541 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    30601 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    30661 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    30721 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    30781 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    30841 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    30901 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    30961 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    31021 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    31081 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    31141 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    31201 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    31261 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    31321 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    31381 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    31441 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    31501 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    31561 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    31621 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    31681 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    31741 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    31801 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    31861 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    31921 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    31981 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    32041 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    32101 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    32161 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    32221 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    32281 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    32341 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    32401 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    32461 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    32521 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    32581 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    32641 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    32701 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    32761 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    32821 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    32881 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    32941 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    33001 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    33061 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    33121 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    33181 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    33241 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    33301 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    33361 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    33421 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    33481 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    33541 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    33601 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    33661 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    33721 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    33781 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    33841 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    33901 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    33961 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    34021 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    34081 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    34141 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    34201 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    34261 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    34321 acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt ctcttgaaca
    34381 ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt tttccgaaca
    34441 aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa aatatggagt
    34501 gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca caacctctcc
    34561 actaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
    34621 gaagctgttt tttgaaattg ttccttgtga ctcggagcta aacggcaggc tcacaacctc
    34681 ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
    34741 aggaactatt ttttccgttt ttttctctaa tactcaggtc aaaacgctga tttcacaacc
    34801 tctgattcac ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaat
    34861 ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
    34921 cctcttattt tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag
    34981 atttgctata atagacgata agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc
    35041 aaattgtaac agattcttgt tgtgatctgc cataccatgt attagcaaaa gaaaaagttg
    35101 attttttatc aatgaatctc caattgaatg atcaagagtt agtagatgac ctcggaaaga
    35161 cattcaatta tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac
    35221 aaatcaatgt tggccgatat ttagaatttt tccgttctta tgcggaaaaa cagattcctg
    35281 ttttatatgt ggctttttcc agtggattaa gcggctctta cagtagtgcg cttcaagcgg
    35341 ttgaattgct gaaggaagaa tatgaagacc ctgagatcta tgtttttgat acaaaagctg
    35401 caagtattgg ggaagggctt ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa
    35461 gcatcgaaga aaccctagag tggctggaaa gtaattattt gaaagtccat tcatgggtaa
    35521 cagtagacga tctgaaatat ctagaacgag gcggtaggat ttccaaagca agtgctactc
    35581 ttggcagtct attgagtgta aagcctatta ttacggtgga taaagaagga aggctgacga
    35641 atgttgacaa agttcgagga agaaacaagt cgatccagac aatcgtcaat gagacggtaa
    35701 aaacaatcgt tgcaccactt gatcaaatcg tttatgtggc ttacgctggg gatctagaga
    35761 gcgcggagaa ggcaaaacgg cttttggaag agcagataaa gatgaaagat gtgaagcttt
    35821 atcccttagg accaacgatc gccagtcaca caggctatgg ctgtatcgcg attttttcga
    35881 tgggaatcag tcgataaaag cagtaactca ttctgtaaaa aagcccagaa attgattgta
    35941 acaaaaacag cgactcttaa aagatagatg agcatcctat ctcttagagg tcgctgtttt
    36001 tttatttttt aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat
    36061 ctgcattttc caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg
    36121 aaacggcaat gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata
    36181 agattgagcc agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta
    36241 aatggttcgt tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa
    36301 gcatgcaacg gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac
    36361 cagaaaacaa cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac
    36421 ttgcatattg acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat
    36481 cctttggtaa ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg
    36541 taatttcacc catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga
    36601 aagcgacctt gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat
    36661 caatcgttac acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt
    36721 tgaatccaac gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt
    36781 caagcatttt tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg
    36841 caataaattc ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa
    36901 gtttagccgc tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga
    36961 ttttaccaaa atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat
    37021 gggatacaac gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg
    37081 attggatatc cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata
    37141 gtgtttgtgc taataacact tttacctttt gtccaccagt taattcactc atttgctgat
    37201 ggtgcaattc ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt
    37261 cccatccatt aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt
    37321 cagtgaaatc ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct
    37381 tgtgtcccat gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt
    37441 tcaatacggc catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat
    37501 cccctgataa cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat
    37561 agcagtttcc gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt
    37621 gcaaactcac atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt
    37681 gacaagtctt atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct
    37741 ttgtgtttca cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta
    37801 ctataggatt tattagtttg caacagcata atttttgacg ttatttataa gagggagtac
    37861 tataaaactg taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac
    37921 tggggctcat tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac
    37981 cctgtcggaa atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg
    38041 atatttgaac tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg
    38101 cccttaataa tcgggatcat cgatattcta gttggcgtct ttttcttatt caatattggt
    38161 gcaggagtcg cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta
    38221 gcgttattta ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc
    38281 atcgtcaata ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct
    38341 gcgttgacat taagcttttt agttggattc tattttatgt tatttggtat cacacatatc
    38401 gtctatgcat ttagataagt aaaatcaaac aaataaaaaa gccggcttcc gattacagtt
    38461 ttactgttgt aattggaagc cggctttttt atcatggaca aatatgcacg aggtgatttt
    38521 taggtgtcga gacaaattta tgcaaaaatg tgagaagcta tatacacaaa ttttatctta
    38581 atactcgaag catagcgcag ttgtcacaac ctcttttcat ataattcatt caatactggc
    38641 aaatcatgga cttcatattt ttctttgaat gcttggattt cttcttcaga atattttctt
    38701 ggatctaaat gcagatcttc tactggcaat gggcgcttgt tattgatctt cacatcgatt
    38761 actacaggac gatctgattt tgcagctgca tcaaaggcaa atcgaagttg cgagtattct
    38821 gtgactgtaa agctatttgc cccgagcgct tctccaactt taccgaaatc tgcatcttct
    38881 aaaaatacac caaatttctt ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa
    38941 gaatcattcg aaaagacaac attaataatt ggaagctgat atttgacttg tgtaatgata
    39001 tcttgcatga ccatagcaaa cccgccatca ccacttaagg taaatacttg tttttctggg
    39061 tagcttagtt gtgcggcaat tcctcctggc acaccattac ccatagttgc gaaccatcca
    39121 gatgtcgtat gtttttgttt accgttcata ttcaataaac gaatagaatg gatagtcgtg
    39181 ttaccgacat ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat ttctttatag
    39241 acagcctctg gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa ccaatttacc
    39301 catttttctt tattttcttg atttgctagg taccagctat cagcagggcg agcatcccct
    39361 aactcgacaa gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa agatgtttca
    39421 tggcgtcgtc caaattttga cgcatcgata tcgatttgga taaattcagc agttggatta
    39481 aaaaatgcac gtccaaatgg gaagtcgcta ccgacaaata agatcagatc ggcttctgcc
    39541 aatgcttcat ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa gttttcataa
    39601 tcatcaggaa taattccttt tgctagaacg gaagcgacaa caggaactga aaaatgttct
    39661 gagaattctt tgatttgcgg aaatccgttt cgcgttcctt gaccaacata aagaactggt
    39721 ttttttgctt tttcaagata aggcaaagca gctagcaaat ctttttcttc tggtaatatc
    39781 acacttgttt tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca
    39841 agatcaacag ggatagtcac gatagccacg cctttatgct cataggcggc tttaatggct
    39901 tcatctacta catgcggaag actttgtggt gtcatgactg tacgattgta tacgctaaca
    39961 tctgcgaaaa taggattttc atttagttcc tggaaagagt tatagttcat ggatgtagaa
    40021 gcaacttgtc ctaagaacgc tactagaggc acatggtcca tctgtgcatc atacaaacca
    40081 ttgatcaaat gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac tttccccgtt
    40141 aattttgcat ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag
    40201 ttgatttcgg ttttttcttg atatagcgca tccataattg aattgaaaga tcctccggga
    40261 atgccgtaaa tatggtcgat accccagctt tcaagaactt ttaccattgc gattcctgca
    40321 ttgatttttg acatggtgtt tcactccttt agcaatgatg atacataaat tttttgtatc
    40381 tagagtatag aactttttat cgaaaatccc aactaattcg tcttttcaca aaaatgtagt
    40441 catgtttttg ggaatatgct agagtagtac atagatagat tcaagagagg ggaatggcat
    40501 ggttgaatgg ctattgagac ttgaaccttg gcagcaagca cttgctggga cagcttttac
    40561 ttattttatg acagctcttg gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga
    40621 agtgctaaac ttgatgttag gatttgcttc tggtgtcatg attgctgcaa gtttttggtc
    40681 tttgttagat ccggctatta caaaagcaga agaaaatgga gatattgctt ggctagttgt
    40741 aagtattggg tttggtttag gcggtgtgtt tttatatatg gcagataaaa cactgcccca
    40801 tatgcatttt ggaccacaac atgaagcaga aggattgcct acccatttaa aacggacgat
    40861 cttgcttgtt ttttctatta cattgcataa cattcctgaa ggtttagccg tgggggtagc
    40921 attcggtgca gcagcaactg cggataatcc gacagccgct atattagccg ctgtttcggt
    40981 ggcgctaggt attggtatcc aaaacttccc agaaggagca gctgtatcaa tcccgttgcg
    41041 acaagaaggt ctgagtcgta aaaaggcctt tgtttacgga caagcttctg gaatcgttga
    41101 accaattgct ggagtgattg gagcgctact agtaacgaaa gtggagctgc tgcttcctta
    41161 tgcacttgct tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat tgatccctga
    41221 agcacagcag acattgtcca gcaaacgtca ttttgcagtt tttggcgtca tgagtggatt
    41281 tatcattatg atgattttgg atgtggcatt aggataaata aaaaaaatat atatgatata
    41341 tcaaataggg ccgcgaactt atatcgcgac cctatttttt tagtaagtta tatcataatg
    41401 aagtgaatgg tatcatacat taataatagt tttttctatg tgtatttcct ttgatccagc
    41461 gaacaagcca gatgataagt gcgattggga ttagaacttt gaccgcaaac cataaaagcc
    41521 cgcttaggac actgacaaaa agactgatga cgaagctagc tgcaattagc actaaaagaa
    41581 ccatgaagat atttaagaac atattttttt gacgcataca aaaccctcct cttgttcatt
    41641 ctttactata gaaaaagtat aacagggatt gtttaaaaac acctccaact gaagactgat
    41701 ttttacttca tctacttaaa tgatcaaaca attgcttttt ctctggttta tcatctaaaa
    41761 tagagcggaa aaggaggaga atatcatgcc taaaaatcgt cttgaagcat ttacagatgc
    41821 tgtaatcgcg attatcatga ctattttagt tttagaactt catgaaccta acggagacac
    41881 gtgggaggcg ctcgctgcat taggttaccg attttttatc tatattgtca gttttatcat
    41941 tttagcgatt tattggaaca atcatcatca tttatttcaa gttgtacata agataaatgg
    42001 acgggtctta tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac
    42061 ttcatggatc agtgagtaca tcaatagcct ggctccagaa atgacttatg gttttgttac
    42121 cttgaccgct aatattgcgt attatatctt ggcaaaagaa cttgtcaaag caaataaagg
    42181 ccacgagaaa gcattgaagg cgatgttgaa taattacaag aaatcgtatc tatcgattgg
    42241 tttgaatgtg ttaggaattg tcttagggta tttagtagcg ccagtagccg tgctgatcgc
    42301 aaacatcgtg attctagctg cttggatcat cccaagtcgt accatcgaat cacaatatcg
    42361 gtaaaatgat ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa taaacgatca
    42421 aatatccgag tgatactagt attcaaagag ggatgcagat ggatcaaaaa agaattcatg
    42481 taggactgat tactagtttt gtcacgatgc ttatttattt ggatcaaggt catttgactt
    42541 ggacagtagt tggtgtcgta ggatttttga cattattcac agcaataaaa aaataaacat
    42601 ctttatgtaa tctga
//
LOCUS       DACDHJ010000006        39913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDHJ010000006 DACDHJ010000000
VERSION     DACDHJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.006"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV223_000259"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV223_000259"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8710570.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV223_000260"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV223_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8710571.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV223_000261"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV223_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8710572.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV223_000262"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV223_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8710573.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV223_000263"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV223_000263"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8710574.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV223_000264"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV223_000264"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8710575.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV223_000265"
     CDS             complement(8053..8523)
                     /locus_tag="IV223_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8710576.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV223_000266"
     CDS             complement(8569..8994)
                     /locus_tag="IV223_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8710577.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV223_000267"
     CDS             complement(9061..9330)
                     /locus_tag="IV223_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8710578.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV223_000268"
     CDS             complement(9341..10936)
                     /locus_tag="IV223_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8710579.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV223_000269"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV223_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8710580.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV223_000270"
     CDS             complement(14489..15049)
                     /locus_tag="IV223_000270"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8710581.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV223_000271"
     CDS             15402..16376
                     /locus_tag="IV223_000271"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8710582.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV223_000272"
     CDS             16546..17946
                     /locus_tag="IV223_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8710583.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV223_000273"
     CDS             complement(18024..18746)
                     /locus_tag="IV223_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8710584.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV223_000274"
     CDS             complement(18825..19331)
                     /locus_tag="IV223_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8710585.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV223_000275"
     CDS             19454..21253
                     /locus_tag="IV223_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8710586.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22931)
                     /locus_tag="IV223_000276"
     CDS             complement(21321..22931)
                     /locus_tag="IV223_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710587.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23186..23695)
                     /gene="mreD"
                     /locus_tag="IV223_000277"
     CDS             complement(23186..23695)
                     /gene="mreD"
                     /locus_tag="IV223_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8710588.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23699..24553)
                     /gene="mreC"
                     /locus_tag="IV223_000278"
     CDS             complement(23699..24553)
                     /gene="mreC"
                     /locus_tag="IV223_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8710589.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24708..25346)
                     /locus_tag="IV223_000279"
     CDS             complement(24708..25346)
                     /locus_tag="IV223_000279"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8710590.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25347..25997)
                     /locus_tag="IV223_000280"
     CDS             complement(25347..25997)
                     /locus_tag="IV223_000280"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8710591.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26010..26909)
                     /gene="rsgA"
                     /locus_tag="IV223_000281"
     CDS             complement(26010..26909)
                     /gene="rsgA"
                     /locus_tag="IV223_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8710592.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27074..29143)
                     /gene="pknB"
                     /locus_tag="IV223_000282"
     CDS             complement(27074..29143)
                     /gene="pknB"
                     /locus_tag="IV223_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8710593.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29140..29880)
                     /locus_tag="IV223_000283"
     CDS             complement(29140..29880)
                     /locus_tag="IV223_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8710594.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29893..31251)
                     /gene="rsmB"
                     /locus_tag="IV223_000284"
     CDS             complement(29893..31251)
                     /gene="rsmB"
                     /locus_tag="IV223_000284"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8710595.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31251..32189)
                     /locus_tag="IV223_000285"
     CDS             complement(31251..32189)
                     /locus_tag="IV223_000285"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8710596.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32182..32673)
                     /gene="def"
                     /locus_tag="IV223_000286"
     CDS             complement(32182..32673)
                     /gene="def"
                     /locus_tag="IV223_000286"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8710597.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32688..35096)
                     /gene="priA"
                     /locus_tag="IV223_000287"
     CDS             complement(32688..35096)
                     /gene="priA"
                     /locus_tag="IV223_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8710598.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35349..35654)
                     /locus_tag="IV223_000288"
     CDS             complement(35349..35654)
                     /locus_tag="IV223_000288"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8710599.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35659..36273)
                     /gene="gmk"
                     /locus_tag="IV223_000289"
     CDS             complement(35659..36273)
                     /gene="gmk"
                     /locus_tag="IV223_000289"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8710600.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36760..37044)
                     /locus_tag="IV223_000290"
     CDS             complement(36760..37044)
                     /locus_tag="IV223_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710601.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37062..37952)
                     /locus_tag="IV223_000291"
     CDS             complement(37062..37952)
                     /locus_tag="IV223_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8710602.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37972..38190)
                     /locus_tag="IV223_000292"
     CDS             complement(37972..38190)
                     /locus_tag="IV223_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710603.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38160..39860)
                     /locus_tag="IV223_000293"
     CDS             complement(38160..39860)
                     /locus_tag="IV223_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8710604.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT        10778 a         8699 c         7256 g        13180 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcac tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gttgattctt ctgttgctga agattcaggc gctgttgatt cttctgttga agattcaggt
    22021 gttgttgatt cttctgttgc tgaagattgt gtagctgatg atgattcttc agtcattgaa
    22081 gattgtgtag ctgatgattc ttctgttgct gagctttgtg cagtagatga agcttgagta
    22141 gcttgtgctt cagcctctgc ttgttcgcgt gcttcttttt cagctttttc ttgtgcagct
    22201 tgttgacgag cttgttctgc tagacgttgt tgttcacgaa tacgagcttg ctctgcttca
    22261 gcttctgctt tttgacggtt caagtcagct ttcttgtctt cagcagttgc ttgttcagca
    22321 gctaatgaag ttttcagtac gttcaaatca gcttgtttag ataaaagatc gcctttttga
    22381 gattccaaag cagcttggtt ttcagctaat tcttttagtt tagcatcgtt ttcagctttt
    22441 ttgtcttcta cagctttttt atcttgtttt tgttgttcca tcaagtcgtt gtttgctttt
    22501 accatcgttg tcattgcttg aacgcgtcca atagcatctg ctaatgaatc agcattcaat
    22561 acagcatcga tgtagtttga gcttgtgttg cttacttgtg cttcgcgtgc ttgtttttgg
    22621 attgtatctt cacgtttttc aatacgttct tgtaaatctg caatgtcttt tactaattgt
    22681 gcagattctt gacgcaatgt atcttgtttt gctaataggt cttgtgcttg tgtgttgatt
    22741 tcagaaactt ggctttctaa ggcatcgatt tgtgattgtg catctgcttg ttgatttttc
    22801 aagtctgcaa tcttttgatc ttgctgttgt atctgagaat caaaatcgtc cgccgcggca
    22861 gcgatcggtg atgctacggc agtcaaggtc attgaacata ccattactgc tgatattaaa
    22921 ctctttttca ctcttcattc ctccgactgg cttaatttaa taaatatcta aatatattta
    22981 agtctttctt aacgatataa aaattgtacc atagcagtgt gtcaacgata taatagttat
    23041 gttacaaaaa tgtttcaaaa caacttttaa aaatgacaaa actgttcgtt tcagaaaatg
    23101 atcatgaaaa agccgcgtta agtctagcat ttcttaagat aacaacgatt ctttttcgct
    23161 ttcgacctaa aaataaaaaa agaaattatt catcactgaa taatttctta aagggaaaaa
    23221 tgaataatac aaatataatc atgttcaaca gcagtgtagg tcctaaaaac cttgtaataa
    23281 aatacgtgtt atttactact gctaatttaa aaatcaactg gaccaccatc gtaaacaatt
    23341 cataacccgt gacaaagatg atcatactga aaaattcagt aaagatgttg acatggataa
    23401 cgtctttcat tacatacatc aaccatacaa ttaaagggag agctactgca tagataccga
    23461 taacgccaat atagtaagcg tcatagatag ccccgagaac aatcgtggta atcagtaaat
    23521 aacgtttttc aaacgccata ctgcaacata ataatgccaa tatcagaaag tgggcatttg
    23581 acatataggt gcctttcgac cactccccta gcattcttgt taaatgacca tctatcaaca
    23641 tcaaaaagaa caaaacgatt ggcagatagt acttcattgt ttctttcttc aacatggact
    23701 aatcctccgc tagccgttgg acaatcgtca caactgacac gtcatacatt tctgcataag
    23761 gtttcacata aacttcccga tccagcccat aactatctgg tttcgtttta ataaccgtac
    23821 cgatcggcaa gttagctgga gaattccctc caagaccgga tgtctggaca acatcccctt
    23881 ctttgatatc cgtatcacca gttaattggg tcaccactaa ggcatggagc ttttcatcat
    23941 agtttttaag caaaccaaac gcttcgccat tagccgaaga tacccgtact ggaaaatgat
    24001 tggagctttc attagaggat gacagtaatt cgattttaga cgaagccgta ttgacctcga
    24061 tcactcggcc aatcaatcct ttttgcgaca tgactgccat atttgcttcg ataccgtctt
    24121 tagaaccctt gtctacgatc agcatgtcct gccaactgtc aggcgaacga gtaatgacat
    24181 ttgccgttac tttttcgtaa ctagtcagcg tctgattcaa ctcaagttct tttttcaacg
    24241 cttcgatttc tctctcgtaa ttcttgtttt gctgtgaaac gtcatcatag ctatcgattt
    24301 tttcttttag ccgctcattt tcggcataag ttgaaaaaag attatggaca gaatctactc
    24361 catttcgtac ccaccgtact ggggcagaga ttgttttatc aaccattgca acactgtcat
    24421 taacgatgga ttggaagaaa ttgggctttc cttcattcgc tcgctgcgca gcggtcacac
    24481 ttagtatcgt caccacgatg ataacaataa ccaaggtaat aataatattt ttattaggat
    24541 tgaacttctt cacaaagaca cctcgaatta attagactct tgtgttcaat tttatcacta
    24601 aatgccgtga aacaaaaggt aagttgctcc tcttaagctt ttttttagca aataatagat
    24661 tctgtcattt ttagcctgaa agacaaattt ttttatttgt ttatggatca atctttgctt
    24721 tgaataactg caatcatacc atctgaaaaa gaaaattctg cggtatctga aagaaattcg
    24781 ttacttgcat aagatgtcgg atattctact tctttatttt ccaatacata cttgctgcct
    24841 tttaatgtaa gattcgagac gggagtcaag cagcaataag ctaaatactt catatctggt
    24901 atctgtctta ctatatgctc tccaggcaaa taataagaca aataattttg acaatctctg
    24961 ataataagct gatgggcaaa gggtttgaag cgaggttcca tacctaacca gagattggct
    25021 aaaagatgat ccaatcttcc gcccgtagct ccaatcaaag tgacttccgc ttgcgggtaa
    25081 cgttcaaatg tttttaagat acctaactgt gtatctgtat cgtctttttc tgcaggggaa
    25141 cgggtatact ctttggcttt tgcaaatact tccttctgct cttcgttcga caatgaatca
    25201 aaatcaccca ctgccagatc tactgggtag cctgcttgaa gcaggtacag cgcaccacgg
    25261 tctatcccga cataggcatc gtatttggac agcagagact gcggccagtt ttgcggatta
    25321 cctccagcag ctaataaaat tttcatttag tctaatgcct cttttaatcg ttcgatttgt
    25381 ccgactggat tttcggctcc atagatatat gatcctgcaa cgaagacatc cgcccctgca
    25441 tctttacaga tttttgctgt ttctggaacg attccgccat ctacttcgat tgcataagtc
    25501 caattgttct ctctacgtag atcagcgagt tccgtaattt tttctactgt ttcttcaata
    25561 aagctttgtc cgccgaaccc aggattgaca gtcatgacta gtacttgatc agccaaaggc
    25621 aaaacatgtt tgatcatgga gactggtgtt cctggattga taactactcc agctttaact
    25681 cctaagcttt ttgtcatttg aagtgcccga tgaatatgag gagttgcttc gacgtggaca
    25741 gtaatgatat ctgctccagc ttttgcaaat gctggaatgt agttttctgg atcaacgatc
    25801 atcagatgga cgtctaacgg taatttagta acaggacgga ttgcctgaac aatatttggc
    25861 cctaatgtga tatttgggac aaatcggcca tccattacat ctacgtggat gtaatctgcg
    25921 cctccttttt ccactaactc aatgtctctt tgtaaattgg caaaatctgc actcaaaatg
    25981 gatggtgcta tcttcattct tgtttcctcc tattttcgtt taccttcttt ttttgcatac
    26041 attggttttc ttttttcgat ttcttgtaag aattgcaaat aattgtcgta acgtgtctgt
    26101 gcgataccac ctgtttctac tctatgtttt acttcgcagc caggttcttt agcatgcatg
    26161 cactctctaa atttgcaatg gacagaagct tctacaaatt caggaaattg tctcggcagt
    26221 tctcctgctt caattgtcaa aaagtcaatc gagctaaatc ctggtgtatc tgctactaac
    26281 ccgccataga taggaatcag ttccacatga cgtgttgtat gccgtcctct tccaagtgca
    26341 tcagaaatct ctcctgttgc taaattcagt tccggtgata tttgattcag tagcgtcgat
    26401 tttcctgctc cagactgacc cataaagacc gatagccgtt ctgggaagta acggatcagt
    26461 tctgtcgtat cacccgtatg attgggtaca atcaccggat agcctatttt agtataggtt
    26521 tgcttgatct cttcgatttt ttctgggtcg tctgacaagt cggtctttgt caaaaaaata
    26581 atcggttcga tcgactcgta ttccaaggta actagaaagc ggtcaagtag attataagaa
    26641 aatgcaggct ctacgatact tgtgacaatg attccaagat caacatttgt gactggtggg
    26701 cgaactaatt gattttttct cggcaagatt tccagaagat agccgtctgt ttgattttca
    26761 ctctcaaaga tcacctgatc tccgacaagc ggcgtgattt tccgattccg gaaatttcct
    26821 cttcctcttg tttgataggt ctctccgttt gcgtatacat agtaaaaacc gctgattgct
    26881 tttctgattt gtcctttgat aagtgccacc tccgtttttg tttattttac catacgaaga
    26941 ggaaagtagg tgttattcca tgcaaacttc tcaaagtata tggcttgaaa attgatcaaa
    27001 agagatagaa acacaaaaaa gagaatgcaa caaatcaatg tcgcattccc ttctgatata
    27061 ttttattcag cacttatttt gaactttctg aggtgctagc tgatgaactt gtggagctgg
    27121 aagattcttc ttttgaagag ttgcttgtgc tactggagct acttgagctg ctagaactgt
    27181 ctgaagagtc tttttcagaa ctagaagacg ctggatctgg tcctttagag taaattacgt
    27241 tcacaaccat tcctttggta attgtcgttc ctgcccctgg tactgtacga atcactttat
    27301 ctttatccac cgtgtttgaa tattcggaag tctcatgccc gatatattct gcaccgacac
    27361 tagctaaata actttgtgct tcgctttttg tatagccgga gatatcattc aatttaggct
    27421 ctgttccttg gcttacgaca aaattgatgt aagtttcttc tgctacaact tcacccgagg
    27481 ctggatcctg actgatgatc gaacctgatg gtacagtgtc gctatagtca taagtagcac
    27541 taatattatt gatattgaat ccttgtgtaa caagccaatt acgaacagtt tcgtagtttt
    27601 ctcctacatg actactcaaa gtcactgttt ctggtccttt gctgactgtg aaagtaatcg
    27661 tgtctgtttt aggatcgact tctgtatctt ctgatggaga ttgcgaaatg atcattccgc
    27721 tttctacctc tgaactgaat tcctcttctt tttgaatcaa gtcagcagaa aaccctaatt
    27781 ctttcaaact tgcttcggca tcttcataac ttttaccact gtaatcatct aatttgattt
    27841 ttttcgtgcc gctactaata tacagagtga cttcgcgttt ttgcttgact gtcgttcccg
    27901 ctgccgggtc tgtcttcact acattacctt cttcgatttt gtcatctgca atttcttctg
    27961 tttttgttgc tacttctaat ttcgcttttg cgagtgtttc tctagcagaa gcttcagaaa
    28021 gatcagaaac atcaggaaca acaacttctc ctcgcccgcc agaagcaaag aatacagctg
    28081 ttccgattcc taaaacagca agaatagcta ataggatcag ccaccatttt ttctgtcctt
    28141 tctttttagg atcaggcaga acttcttcct cctctggtaa ggtttcgttt tctggcttgt
    28201 cttttggcag aggcatagat ttgaatgaat caggcattgg tgcatcttcc gtaaggggtg
    28261 tgataacttt tgtttcatta tccatcacat gaggctgcca tttttcttcg tttgcacgag
    28321 ctggtgataa tactgtagaa agatcatcag acatttcctc ggctgactta taacggtctg
    28381 caggttcttt agccgttgcc cgtaaaatca cgttttccag tgcttgtggc acatttggat
    28441 caagtgcttt tatagatgga agatcatctt gaaaatgttt taaagcaatt gttacagcag
    28501 attcaccatc aaacggtacg ctacccgtca acatctcata aaggatgatc cctaaagcat
    28561 aaatatctga ctgcttcgtt gccatacttc ctctggcctg ctcaggggac agataatgaa
    28621 cagatcctag catcgtattc gtctgcgtga tcgatgtttc agataaagcg atagcaatcc
    28681 cgaaatccgt gatttttaca acaccttcat tatcgatcag tacgttttgg ggtttcaaat
    28741 ctcggtggat gatttgatgg ttatgagcta aagaaatagc agaaaggatc tgctgcataa
    28801 tatttactgc tgtttcataa ggaacaggat aatgtgtttg gatatatcgt ttcaagtcca
    28861 ttcctttgac gtattccatc accagatact gcatgttgtc ttcttcacca acatcgtaaa
    28921 cactaacaat gtttggatga acgagttctg ttgctgctaa agcttctctc tgaaatcgtc
    28981 gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac tttaactgct acatcacgat
    29041 ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc accgctaccg atatttccag
    29101 tgatttgata acggccattt aattttttgc ccaactcgat catacactgt cctccttata
    29161 ttcgataaga agaacggtga tattatccgc tccgcctgct tcattggcac gatcaatcag
    29221 ctcagttact ttatgagaca atgggccttc ttgattgatg atcgttccta tttcttcttc
    29281 agacagcatg ttcgtcaagc catctgaaca taacagcagt cgatctttta gttgccaaag
    29341 atgagtagaa acatctactt cgactgttcc aggcatgccg acagaacgtg ttaaaatatt
    29401 tttccttgga tggttcactg ccatttcttc gctgatttct cctgatttga ctagttcatt
    29461 gactaaagaa tgatcctccg tcaattgcat gattttatct tcacgaatca aataagctct
    29521 gctatcccca acattggcaa tcgtgaacca gtcatctgac agagcggccg ctacgattgt
    29581 tgttcccata ccgttatatt ccggctgttc ttgtccacgc tgataaatca gcatattttc
    29641 ttcttgaatc gattgaatga accattgggc gattttttca tcgtcaacag cttcttgttt
    29701 ttcccaagct tcgcctagat tcgttaccgc catctgactg gcaatgtctc cggcacgatg
    29761 accacccatc ccgtctgcta atacagcaag cttgagacct gaacggctgg taaatacact
    29821 ggcatagtct tgatttgtgt tacgtcgtct tcctacatct gattgatatt caatctgcat
    29881 tttttcacct cattactttt tgcgcaaaca acaaatgaaa aatccatctg tgtaaaactg
    29941 atgcggatac aaagtcaaca ttttctcttc gattgatgat tgtagcatag gattggtcaa
    30001 aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa gcagaaacaa cgtcttggtt
    30061 ttcttctttt aagatcgtac aagtgctata agtcagaata ccggaagact ttaaagttgg
    30121 tgcgcagctt tctaaaattt ccaattggat ttttggtaaa ttcgctaatt catccggctg
    30181 ttttttataa cgaatatctg gttttcgccg aagcagccca agacctgaac aaggcgcgtc
    30241 tactaaaatc cgatcaaact gctctgcttc gaactgttta ctgacttctc tagcatccat
    30301 cttttgagcg tagacagcag aagagacacc tagacgtgct gcattatctt caatgagttt
    30361 gactttgtgt tcgtgtacat ctaaagcgac cacttctcca ccgcatgacg gatctaaaaa
    30421 agaagcaata tgcgtcgtct tacctccggg tgctgcacag gcatctaaga catgatgttc
    30481 aggctcgatt tgcatagacg gcgctacaag catcgaactt tcatcttgga tcgtcatgac
    30541 gccatcgtgg aacaatgagc tagaagctaa atgccctttt ttcgctacta taccgtatgg
    30601 actgacttgg cttttttcag ctgtgatctc ttcttcttgc aaaacagtga tggcctgatc
    30661 tctggaaagt cggcgtgtat ctactcgggc actagcatgg ctaggctgat atagcgataa
    30721 accgagcttt tcagtttctt cgtatccgat ttgatccacc agtcgtttcg tcatccatag
    30781 aggcaggctg attttgacag aaagtctttc gataggatca gcaatgcctt gcagatctgg
    30841 tgctccttga cgctgataat tcctcaagac accatttaca aatttccctg tcccaggatt
    30901 tccttttact tttgcgattt cgaccgcctc gtttaaaata gcatgtgcag ggactttatc
    30961 taaaaattct aattggtata cagaaagata aagaagcatc ttgacccagt catccacttt
    31021 ttttgcttta gcgatcaatg gtgcaacgta aaattccaat agtcgctgat ggctaatcgt
    31081 accatagatc aattctgtaa ataaacccac atctttttga tttagctcgc tttgtttgat
    31141 catctcattc aacaaaagat tcgagtaggc gccacccttg ttcactcgct ctaatgtcag
    31201 taatgccata tatcgtactg attttttcat ttttttaggg atttttttca ttatttcacc
    31261 tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga aatcatgtac ggcctgtttt
    31321 cctttaccag ctggctgtaa ttcattgatg gctaagacgg tctgtttacc gcaagcaatc
    31381 catagatttt ttttgtcttt ccggatgatc gtaccaggtg ctttgtctgt agtttctgcc
    31441 aatacttcaa ccgataataa tttccagcgt atgccctcat aggtcgtgaa tgcaattggc
    31501 caaggacgca ttccgcgtac ttggttgtca atttcttctg cagttttatg ccaatcgatt
    31561 gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt ctgactcgtc ttgaggtact
    31621 ggtttttgtc catccaaaat attgggcaat gtttctagca atagctttct tcctaaaagg
    31681 ctaagctttt caaacatcgt ccctacatca tcttgtttag tgattggtat gctttcttgc
    31741 gcatagattc cgcctgcatc catttttttg atcatttcca tgatcgttac accagtttct
    31801 ttctccccat tcatgatggc ataatgaaca ggcgcacctc cccgatattt aggaagtaaa
    31861 gaagcatgga cattgattgc ccctaatttt ggtacttgca ataacttttc aggcaagaat
    31921 tggccaaatg ccgcagtgac gatcacatca ggcaccaatt caatgatttc ttccatttct
    31981 ggcgaaccgg agattttctc tggctgcagc acacggatac cgtgcttcag agccgctttt
    32041 tttaccggtg tcggcgtaat tactcgtttc cgcccaactg gacggtcggg ctgtgttacc
    32101 actgcctgga tctcgtaacc tgattctatc agcccttcaa gaatcggcac agaaaaagca
    32161 ggtgtgccca taaatactag cttagtcatc aagatgttcc tccatatatt cttcaagttc
    32221 ttcttccggg atctgctgta tcattttctc aatgaacaag ataccgtcta agtgatcgat
    32281 ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc acttctatct cttctccgtc
    32341 tcgatcataa taacgcacgg tcacttcatc tgctcgcttg accgttccat agacatgcgg
    32401 aatactgaga caaccttcta cgtcaaggct ttcacctttt gcttcgatga tttctgggtt
    32461 aatcagttca aatttctcgc cttcatctac ttcgatcaca gcaattctct tgttttgtcc
    32521 tacttgagga gcagcgatcc ctattccgtc attcgcaatc attgtctcat ataaattgtc
    32581 taataaagcg attgtctcgt ccgtgattac atcgattggc tgagcagttc tttttaattt
    32641 atcattaggg tggatcaata taggatagcg cataatttgg ccctttctta aataaagttt
    32701 aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct gttctttctg gcttccattc
    32761 aaaatttcat ccaaaacatg aggaagattc ggttcatgtt tatatttgat gattagttga
    32821 taataatatc ggtttttcac tctcaaaatc atgctgggcg ttggccctaa tagcaagctt
    32881 tgagggctta acacttcttt tagctgattg gcaatctgaa agattttctt tgccgctact
    32941 tgttcttcag gatggctggc ggttattttc acagtaaagt aataaggagg atagcctcca
    33001 cgatgccgta catgcatttc ctgctgataa aactgctcat aattttgttt tttagccaat
    33061 tctatggcgt aatgttgagg attgaatgtt tggatcacga cttcgcctgc tttttccgct
    33121 cgccctgctc gtcctgaaac ttgggtaagc aattggaatg tatgctcact agaacgaaaa
    33181 tccggtaaat tcagggaggt atcagcattc aacaccccaa ctaatgtcac ttctggaaag
    33241 tccaaacctt tagcaatcat ttgtgtccct aataatatat ctgcttcttt tgcaccgaat
    33301 ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg tatctacatc catacgcaaa
    33361 atcctagctt gcggataaag ttctcggagt tcctcttcta ctttttgagt acctgttcca
    33421 tagtagcgga ttttattgcc gccacaatta ggacagcgat tgggaatagg ttcttcatgt
    33481 ccgcaataat ggcagcgcat tgaacgtgta tccatatgca gtgtaagaga gatatcgcaa
    33541 ttaggacatg gaagaacaaa tccacagtcc ctacacatca caaaagacga atatcctcga
    33601 cgattgagca gtaggacagt ttgttctttt ttgtctaatc gattttgtat tttttcctgc
    33661 aaattagcag aaaaagtaga ggtccgatga ttctgaaact cttcacgcat atctacgatc
    33721 tcaatagctg gaagctgtgc agctgctttg gctcggtggt ttaactgcaa taactgataa
    33781 acgccttttt gagctcgtgc tcttgattct aaagagggcg tcgcgcttcc tagaactatt
    33841 gggcaatgat gatatttact gcgccaaatt gctagatctc tcgcatgata acgcggtgtc
    33901 tcatcttgtt tgtaacttga ttcatgttct tcatcgataa tgatcagccc gatatttttc
    33961 agcggggcaa aaatagcaga tcgtgcgcct actactactt gcgcttcttc ccgctcaatc
    34021 ttgcgccatt catcatattt ttcaccttgg gaaagtccgc tatgtaagac cgcaactgat
    34081 tctccgaagc gtcctttgaa tcgctcgacc atttgcggag tcaatgcgat ttcaggcact
    34141 aacataatag ccgttttatt ttctgacaaa acatcagcga ttgcctgaag atatacttct
    34201 gttttcccgc tgccagtgat tccctccagc aaaaagacct gatctttttg ctgctgacct
    34261 gctgtgatga tttgttcgac agcatttttt tgttctgcat tcagttccaa tgccgtcgtt
    34321 tggtcaaata ctcggtcttt ataagggtcg cgataacgtt ctgattctat aaactccagc
    34381 cagccctttt cttttccttg attcaagaca gcagtactga ttcctttatt tttgaaataa
    34441 gcaattggtt cttcttggct cagttcagat aaacacaaaa gaagttcttt ttgtttcttg
    34501 gcattttgcc gtaattttgc ccattcttct tcgatttgct tttcagttaa caaagaacgg
    34561 accaaacgaa tcgtttttac tttatttcgt gtatggactt catatttgat atctactaat
    34621 tgctcttggc gccagcgaat caattgagaa aggctgcctt cttcttctgc ttccttccag
    34681 gaaatcactt ctcgctgatc aaacaatacc tgaacttctg gtgtgtctgc caaaggatag
    34741 atcaatttat catattctgc tttcatcaca ctcggaagca tcgtttgtag acaagtgatt
    34801 ttgaatgcgt aagtaatttt tttcatataa tcagctaatg aaagtaattc ttcattgacc
    34861 accggagcaa ggtctagcaa acgaataact gccttcaaag atggattagc tgattcttct
    34921 gatttttgga tagccataac aaatccttgg acatgacgat tgccattacc aaaagggact
    34981 tctactcgca tcccgacttg caaagctgtt tccatttcag agggaaccaa ataagtgaat
    35041 ggctgatcgg tctgcatagt aggtacgtcc acaatgactt cagctgttaa tgtcatggtt
    35101 cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa aagttccttg cctattttac
    35161 tagagatcga tggatttgtc ttatcttctg ccaaatccta acaaaaaatt aacagcagat
    35221 aagaaaaaaa agaactgtgt caaaaagttc agacacagcc ttttctacca gacaataagt
    35281 ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct ttttagatgt tcgctctatt
    35341 tttctttatt acattttttg ctcttcacgg atacgtgatt ccaattcgtg ttgttcacgc
    35401 tctttttgtg cttgacgttc ctcttcttcc atcttcaaac gttcacgttt taattcagga
    35461 tgtggatcat tgactacatt tcctgcatcg atttcttcta aagcttgacc gacgcttttt
    35521 actgaatcaa aagaatctag agtaggctgt gcacttgcat ccaattcatg cgcgcgttta
    35581 cttgctaaaa tcactagaga atattttgaa tttacacgat ctaatagtga gtcaattgaa
    35641 ggttttaaca tcataagact acatctcctt taacatttta atatatttac cgattacgcg
    35701 atctactcga aaatgttcac tggcaataat gtttttgatt cgttcaactg ctagcggcac
    35761 ttcatcattt acaacagcat agtcgtaaag agccatcatt tcgatttctt cttttgcaac
    35821 tttcattcgt ttttcgatta cttcatctgc atctgttcct cggcctacga tccgtgattt
    35881 taattccgct aaatctggcg gagtcaaaaa gatgaataca ccatcgggca ctttttcttt
    35941 gacttgtttt gctccttgga cttcaatctc caagaaaaca tcttttcctt catccaatgt
    36001 cttgttgaca tatgacaaag gagttccata ataattcccg acatattctg cgtattccag
    36061 catctctcca gcttcgatca ttgattcaaa ctcttctttg cttcggaaga aataatcaac
    36121 gccttcgacc tccccttcac gcattttacg ggtagtcatt gagatcgaat actgaaaatc
    36181 attatcctca ctctcaaaaa ttgcttttcg tactgtgcct tttcctacac cggatggacc
    36241 agacaacacg attaataatc cacgctctga catgacgacg tcctttcaac tagtcttttt
    36301 gtacttatcg tttattttgc actttttttc tgcaactttc aaggttatct ttaattatag
    36361 ctagaaattg gtaaaaaagc taccctgtac caagaggtcg taaaaaagct gtcctgcatc
    36421 aagtaataag aacagccaaa cataaacagc ttctcatgtt tttcgtttgg ttggggttat
    36481 tatcgcagat gcaagctttt gaacgccgtg tataagaggt cgtgaagaag ccgattagtt
    36541 tcgagtatga agaaaaattt tgtgaaaata gtttctcaca tttttgcata aatttggctt
    36601 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
    36661 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
    36721 ttctgtttat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
    36781 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
    36841 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
    36901 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
    36961 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
    37021 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
    37081 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
    37141 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
    37201 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
    37261 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
    37321 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
    37381 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
    37441 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
    37501 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
    37561 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcac gaagcgttcc
    37621 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
    37681 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
    37741 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
    37801 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
    37861 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
    37921 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
    37981 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
    38041 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
    38101 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
    38161 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
    38221 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
    38281 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
    38341 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
    38401 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
    38461 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
    38521 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
    38581 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
    38641 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
    38701 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
    38761 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
    38821 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
    38881 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
    38941 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
    39001 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
    39061 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
    39121 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
    39181 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
    39241 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
    39301 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
    39361 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
    39421 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
    39481 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
    39541 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
    39601 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
    39661 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
    39721 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
    39781 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
    39841 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
    39901 aaaggtcatg agt
//
LOCUS       DACDHJ010000007        34719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDHJ010000007 DACDHJ010000000
VERSION     DACDHJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.007"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            150..830
                     /locus_tag="IV223_000294"
     CDS             150..830
                     /locus_tag="IV223_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8710605.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            920..1954
                     /locus_tag="IV223_000295"
     CDS             920..1954
                     /locus_tag="IV223_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8710606.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1968..2303
                     /locus_tag="IV223_000296"
     CDS             1968..2303
                     /locus_tag="IV223_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710607.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2287..4767
                     /locus_tag="IV223_000297"
     CDS             2287..4767
                     /locus_tag="IV223_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710608.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4823..6382
                     /locus_tag="IV223_000298"
     CDS             4823..6382
                     /locus_tag="IV223_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8710609.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6379..7743
                     /locus_tag="IV223_000299"
     CDS             6379..7743
                     /locus_tag="IV223_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8710610.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7914..8984
                     /locus_tag="IV223_000300"
     CDS             7914..8984
                     /locus_tag="IV223_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710611.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8999..9667
                     /locus_tag="IV223_000301"
     CDS             8999..9667
                     /locus_tag="IV223_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710612.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9687..10265
                     /locus_tag="IV223_000302"
     CDS             9687..10265
                     /locus_tag="IV223_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8710613.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10666..11577
                     /locus_tag="IV223_000303"
     CDS             10666..11577
                     /locus_tag="IV223_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8710614.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11955..12872)
                     /locus_tag="IV223_000304"
     CDS             complement(11955..12872)
                     /locus_tag="IV223_000304"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8710615.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13074..13511
                     /gene="zapA"
                     /locus_tag="IV223_000305"
     CDS             13074..13511
                     /gene="zapA"
                     /locus_tag="IV223_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8710616.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13569..14117
                     /locus_tag="IV223_000306"
     CDS             13569..14117
                     /locus_tag="IV223_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8710617.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14232..16592
                     /locus_tag="IV223_000307"
     CDS             14232..16592
                     /locus_tag="IV223_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8710618.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16679..16993
                     /gene="trxA"
                     /locus_tag="IV223_000308"
     CDS             16679..16993
                     /gene="trxA"
                     /locus_tag="IV223_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8710619.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17175..18953
                     /gene="uvrC"
                     /locus_tag="IV223_000309"
     CDS             17175..18953
                     /gene="uvrC"
                     /locus_tag="IV223_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8710620.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19098..19997
                     /locus_tag="IV223_000310"
     CDS             19098..19997
                     /locus_tag="IV223_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710621.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19990..21252
                     /locus_tag="IV223_000311"
     CDS             19990..21252
                     /locus_tag="IV223_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710622.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21413..23311
                     /locus_tag="IV223_000312"
     CDS             21413..23311
                     /locus_tag="IV223_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8710623.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23641..24360)
                     /locus_tag="IV223_000313"
     CDS             complement(23641..24360)
                     /locus_tag="IV223_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8710624.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24697..25029
                     /locus_tag="IV223_000314"
     CDS             24697..25029
                     /locus_tag="IV223_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710625.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25016..27019
                     /locus_tag="IV223_000315"
     CDS             25016..27019
                     /locus_tag="IV223_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8710626.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27038..27508
                     /locus_tag="IV223_000316"
     CDS             27038..27508
                     /locus_tag="IV223_000316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8710627.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27529..28113
                     /locus_tag="IV223_000317"
     CDS             27529..28113
                     /locus_tag="IV223_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8710628.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28128..29114
                     /locus_tag="IV223_000318"
     CDS             28128..29114
                     /locus_tag="IV223_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8710629.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29104..29415
                     /locus_tag="IV223_000319"
     CDS             29104..29415
                     /locus_tag="IV223_000319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8710630.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29437..31218
                     /locus_tag="IV223_000320"
     CDS             29437..31218
                     /locus_tag="IV223_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8710631.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31211..32587
                     /locus_tag="IV223_000321"
     CDS             31211..32587
                     /locus_tag="IV223_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8710632.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32594..33229
                     /locus_tag="IV223_000322"
     CDS             32594..33229
                     /locus_tag="IV223_000322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8710633.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33259..34614
                     /locus_tag="IV223_000323"
     CDS             33259..34614
                     /locus_tag="IV223_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8710634.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        11478 a         5976 c         7445 g         9820 t
ORIGIN      
        1 aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca aaaagggtca
       61 taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc tactatactt
      121 aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg acattattac
      181 tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt caagcgactt
      241 caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt aaccctactg
      301 atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc cagacaggtc
      361 ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt gaaagcggaa
      421 caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa gtatcagatc
      481 gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt tcggtttcag
      541 attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga acatccactg
      601 ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta actgggttga
      661 gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt ctgggaacat
      721 ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca gcagctggaa
      781 cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag agtatgaaat
      841 aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg cagttgttgt
      901 tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt ttttttctat
      961 tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag caggagactt
     1021 tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt attttgattt
     1081 attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt cttcagatga
     1141 agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg gaacgatcga
     1201 ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc gtgacgtttt
     1261 gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg tacctataga
     1321 agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc atgtttcacc
     1381 taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa atcgtattgc
     1441 ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg atctgaaact
     1501 tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc gtttccaaaa
     1561 tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt atgaaaatca
     1621 gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga attttcactt
     1681 gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag aaatcatagc
     1741 aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca aaaaagaaaa
     1801 agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt tcccatggct
     1861 ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt ttttgtgggt
     1921 ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg aaaacatcga
     1981 tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag ccagtccaag
     2041 cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt ccgcatccaa
     2101 gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat aaagaatcta
     2161 atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt tatttgatca
     2221 ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag gaaaaggagg
     2281 atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt tacttcgctt
     2341 tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc aacgcctcct
     2401 gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg tggacgtagt
     2461 gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc taaattgact
     2521 gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt ccgttttcat
     2581 tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac tgcacaagga
     2641 tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga tgtattaaaa
     2701 gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa ggttactagc
     2761 ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc gtgggataca
     2821 aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa cgtggacgat
     2881 atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa cggagataaa
     2941 tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc tatgtctaaa
     3001 aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga tcgtatgttc
     3061 aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg cggaaacttg
     3121 aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa tagttacatc
     3181 tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga atacgtggat
     3241 ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat ggggctgact
     3301 gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa ctattttaca
     3361 ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac aagtggattg
     3421 aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt gctttctcct
     3481 gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc aacaaactta
     3541 acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt cacggccaat
     3601 tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc gaaaggaacc
     3661 caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc aaataacggg
     3721 ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata ttattttgat
     3781 tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta caaatggcag
     3841 tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt tccatctgtg
     3901 agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga tcccctgtct
     3961 gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa atctggggat
     4021 gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta tatcaatggt
     4081 tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg agtttctgtc
     4141 ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa ctatcaatta
     4201 aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc tgtactttcc
     4261 agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca aacatctgct
     4321 aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga tgattttaca
     4381 tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa aggcgatttt
     4441 tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact gacaggtaca
     4501 ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa attatacttc
     4561 aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat ttatgagagt
     4621 gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg tttactctta
     4681 gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat ggtcacatgg
     4741 gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct gtgataactg
     4801 tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat cagaaatgct
     4861 tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac ggcaaatcaa
     4921 acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg tacttacccg
     4981 cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag ctaacttgga
     5041 aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa tcagacatgg
     5101 aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta caaaatatca
     5161 gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg tcatggaact
     5221 taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt ctttaaatga
     5281 gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa ttcgttattt
     5341 ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag aatacagtga
     5401 tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg ctattacaga
     5461 agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa tgcgctgtac
     5521 gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa cgacacaatt
     5581 tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct ttccttggaa
     5641 tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat tagatacaca
     5701 taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg tagtagctca
     5761 attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg tttttccaga
     5821 agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt ttgcctattt
     5881 tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa ctgaagtaga
     5941 agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg ctattgttga
     6001 ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct atgcatctat
     6061 tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg atcttcctta
     6121 tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc tgaatacgat
     6181 caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt tgactagcca
     6241 attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta gcaacgaata
     6301 taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag aaaaattgaa
     6361 tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga aggcatcaca
     6421 gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat cgatgaagat
     6481 cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa aggaaagata
     6541 cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg ggcaaaactg
     6601 atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat cctcgcatcc
     6661 aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac aggttcctct
     6721 cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa aaatctagtg
     6781 caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat cttcggtgct
     6841 ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat tattcatcta
     6901 gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat caacagtttt
     6961 caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt gatcgattgg
     7021 aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat aaaggcagat
     7081 atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga aaaagtggct
     7141 ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga tccggacatc
     7201 tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca tctcttttta
     7261 cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt gagtggtcag
     7321 ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga ctacttttta
     7381 ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac cggttcgtgt
     7441 tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt aaatgcaaag
     7501 ctgatctacc atccggatac attagaaata attggcggac agctgataag ccaagaattc
     7561 ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac gattccacag
     7621 ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca ctacctcaat
     7681 gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga taaaagactg
     7741 tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc aatcgagaat
     7801 aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc gccaaaaatt
     7861 tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt taaatgtttt
     7921 tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca ggggtgatgt
     7981 ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta gctcaagata
     8041 atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa gatgcaaatt
     8101 caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa ggtgggaaag
     8161 tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca gaagaaggaa
     8221 aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc aatcttgttg
     8281 aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg aaagaagaat
     8341 atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg tttaaactaa
     8401 ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca accatcaatg
     8461 cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg attaacgcaa
     8521 tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc aaaatttcta
     8581 aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga tttatgatcg
     8641 gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta ctgacgatgc
     8701 aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga tttattgcgc
     8761 gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt ggtctattag
     8821 gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc aattggctgt
     8881 tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca ctattttctg
     8941 ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga ggaagaacat
     9001 gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc aagtagttaa
     9061 agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa tcatcggtcc
     9121 ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga cgcctagcag
     9181 tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg aacgcgttaa
     9241 actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc cttttctgac
     9301 agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc aaaaagaagc
     9361 aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc cagaagaact
     9421 atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg atccgtccat
     9481 tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag aagtagtcga
     9541 tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta ctcacgatac
     9601 acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat tgaaagaaaa
     9661 agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat acacagcaaa
     9721 aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc acttcaacaa
     9781 gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat ttcggtgata
     9841 aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga aaagctttga
     9901 gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa atggcgcagt
     9961 ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt gaaagcaatc
    10021 tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat cgtgaacctt
    10081 ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca aaagaaatcg
    10141 cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa agacctgtgg
    10201 aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta ttaggagaag
    10261 cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat gacgaagatc
    10321 gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt tattctctca
    10381 tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt tttaatttaa
    10441 taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta gatgaatcac
    10501 cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc attttagcag
    10561 taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact taagctgatt
    10621 ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa aaaaataatt
    10681 ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga tgtttttggt
    10741 caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac aaagacaaag
    10801 gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa tgaccgtaca
    10861 ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg agagctggcg
    10921 gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg ctatactgga
    10981 tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa accggaagag
    11041 ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac tggggcatta
    11101 agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat aaaagtaaaa
    11161 aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga tgaattagta
    11221 cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt tatcagttta
    11281 cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata ttattactat
    11341 ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct gcctctggat
    11401 tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca accttctatt
    11461 ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt aacagacagt
    11521 tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt tcagtaataa
    11581 ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg cttattatcg
    11641 cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg ttcaactctg
    11701 agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    11761 accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca acgttttgtt
    11821 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
    11881 ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt gacatgactt
    11941 ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt ggcaaaatga
    12001 agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt tgccgctact
    12061 tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc tgatgctttg
    12121 tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac agctaaatga
    12181 tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt ttctaaacgc
    12241 gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc ttctggtttt
    12301 tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc gactttcatc
    12361 cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa gacaagcagt
    12421 ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct atcttttaac
    12481 tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg tttcacataa
    12541 gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc actacctaaa
    12601 acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg cttggcttgt
    12661 ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc ttgaaacatc
    12721 acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc aaagctagta
    12781 taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg actaagttgg
    12841 tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt atacataaaa
    12901 ctataccgca actcacgact tttcgttttc taagagattg cgagacatag cattttttta
    12961 aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg tgaaagaacc
    13021 aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt cgtatggcgc
    13081 acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt atcggacggg
    13141 aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta gcagaattga
    13201 aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct gtcaatgcgc
    13261 tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa acagcagaat
    13321 taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc attgaagcaa
    13381 tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat accattaaaa
    13441 atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt aaacaaaaat
    13501 cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag aagagaaagg
    13561 aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt ttttctggag
    13621 cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg tcatttattg
    13681 ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt ccatacccag
    13741 ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt cacctagatg
    13801 aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg ctgcttaccc
    13861 gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga caggtagact
    13921 ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt ttgattttgc
    13981 agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag catagtttcg
    14041 ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag ctttgggtaa
    14101 cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg tacagagtag
    14161 tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat agtgaaagat
    14221 aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa gtcaaacaga
    14281 tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa ttggtaccag
    14341 tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga ttgaaagtgc
    14401 aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc catatgaaac
    14461 ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc agagtattgt
    14521 ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa atcgaatttg
    14581 cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc cgccgtttaa
    14641 aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag ttaaagagca
    14701 taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat gggatcgtcc
    14761 gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat gaccgctacg
    14821 ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac gatcaaagtg
    14881 cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat aatcgtttgc
    14941 gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa ctttctgctg
    15001 aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt aaaatggatc
    15061 tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg ggaatcagtg
    15121 aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag gaaaaagtag
    15181 tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca ggacctaaca
    15241 caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg gggcaagcag
    15301 gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa gtatttgcgg
    15361 atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct catatgacga
    15421 acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt gacgagctcg
    15481 gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta gatgatcttg
    15541 gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa gtttatggat
    15601 ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca ttaagtccaa
    15661 cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc tctagccgat
    15721 taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat gagagccaag
    15781 atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag accgaatatc
    15841 tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg aaagaagctt
    15901 acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa aaagcaaatg
    15961 aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt cgtaaaatgc
    16021 aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca aagacccaat
    16081 tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag aaggcgaaag
    16141 aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc caaagaggga
    16201 cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta aaaatgaatg
    16261 tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa cgagttatcc
    16321 atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt cgaggaaaac
    16381 gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt ctagctggtt
    16441 atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag ggaattactg
    16501 attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca aaccaaggcg
    16561 gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc agatggttcg
    16621 ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg tctttgatat
    16681 gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac ttgtattgat
    16741 tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt tggatcagtt
    16801 ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg atgaaaatcc
    16861 agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga aaaaagatgg
    16921 tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc aaatgatcga
    16981 tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa tccagttccg
    17041 tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt gagtataatt
    17101 acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta attgaatatg
    17161 aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt cctgatcagc
    17221 ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc aaagccaaaa
    17281 tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa acggaacggc
    17341 ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc gaagcattgc
    17401 tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg ttaaaggatg
    17461 ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg gtgattactc
    17521 gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc ggtgcggcga
    17581 atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa cctagtcaga
    17641 cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat tatttcgacg
    17701 tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta aacggaggac
    17761 acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa aacatggagt
    17821 ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc atgactcgcc
    17881 aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg attgataaag
    17941 gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa cgagatgttt
    18001 caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc ggtcagtttt
    18061 atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg atcgacaagc
    18121 ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga gaaaagaaaa
    18181 aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg tttgatctga
    18241 ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac gcgatgaata
    18301 tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg acgaatcctg
    18361 tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat cggaaatata
    18421 aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc atctatcgca
    18481 gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg atcgatggag
    18541 gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga atcgatattc
    18601 ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta ttcggtacag
    18661 atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc cagcgaatcc
    18721 aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt aaaaacagtt
    18781 ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa atgctgctta
    18841 aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta caagaaatcg
    18901 gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa tgaggaaagg
    18961 gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa gagcgtttac
    19021 gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat gtgatttctt
    19081 gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt gggaactaca
    19141 cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg ctaattggcc
    19201 aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg acacaagacc
    19261 aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat gtcatcggtt
    19321 atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa ctgctttatt
    19381 ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac ttttggatgg
    19441 aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct aaaggaaatc
    19501 agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc attttggatg
    19561 agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga atattggaat
    19621 tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat gtagaaaaaa
    19681 tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga aaagtacatg
    19741 agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta agccaacagg
    19801 aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat acgttagaga
    19861 ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact cagttcggct
    19921 acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa ttgaaagcag
    19981 gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa aaatgttaag
    20041 tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat catttatgta
    20101 gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt ggctgataat
    20161 gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt caaagcagtt
    20221 gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc gtatttagta
    20281 gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc attaggacaa
    20341 agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct tcgagcgagt
    20401 caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag tttgactcga
    20461 caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa ctcgacagtc
    20521 caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt gacgtatgcg
    20581 cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga agtgatcctg
    20641 tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt acttgttgca
    20701 ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca attcaaagat
    20761 atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat tggaccattc
    20821 ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt tttagcagct
    20881 ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt gccagtgact
    20941 tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga tccaaacaat
    21001 atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat gccgattcgc
    21061 ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt acttgtagcc
    21121 gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt actcgtttat
    21181 agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag gaatgaacga
    21241 aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt gttcccagct
    21301 tctttttgta tagacctatt ttatttattt tactatattg gatatatatt cattgaaaat
    21361 agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt taatggaccg
    21421 ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca cggaaatcat
    21481 caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga gtgatataca
    21541 tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa
    21601 gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat tcactatttt
    21661 tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag ataaatcgca
    21721 gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata cccggtcaaa
    21781 agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga
    21841 tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac ttgttttgat
    21901 gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat ctgtcatcga
    21961 tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca aagtgatgga
    22021 tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata ttttgtggat
    22081 tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg cagcacgcta
    22141 taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat tgttttcttt
    22201 tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta aaaatctatc
    22261 cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc ttcaattcaa
    22321 atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc gtaaattgct
    22381 ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct atgagttgaa
    22441 aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg aagaggaaca
    22501 aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc aaaaacatat
    22561 gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tgctgtttca
    22621 tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag accaatctta
    22681 tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg caaaagatct
    22741 gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg ggccattttc
    22801 gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca cagagcctca
    22861 tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt ggttcagtga
    22921 aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta atgggcacac
    22981 gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag tatttgtcat
    23041 tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt attcactatt
    23101 atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta ttgaacaaca
    23161 atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc aactaccacg
    23221 caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta cccagctcaa
    23281 aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat tgttccatag
    23341 actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac ggtgttcttc
    23401 tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg ttttttgaaa
    23461 ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat aaacggtgtt
    23521 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa gccattttca
    23581 caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc agaaagtcta
    23641 ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta cacggtgggt
    23701 aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc tttgaaccgc
    23761 ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa gatctttgac
    23821 tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc ttaacaaact
    23881 gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct gttgttcatg
    23941 ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt ccaaaacgac
    24001 attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt tcaatgcttc
    24061 caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta gctttggatg
    24121 aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa taagtttgtc
    24181 gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa taagaggcaa
    24241 tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca cttgatttcc
    24301 ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga ttggtttcat
    24361 ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt aaactatgta
    24421 agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaaatttcgt
    24481 gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag
    24541 aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat
    24601 tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa
    24661 aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg
    24721 gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact gcggcagcag
    24781 ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac aaactatcga
    24841 caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga attagaaaaa
    24901 gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa agatcaatat
    24961 caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga aacttatggc
    25021 cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt
    25081 acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa atagaacaaa
    25141 caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag aagcaaaatt
    25201 aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca tacaacacca
    25261 cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagattt
    25321 ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc ttgatttaaa
    25381 agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg aagaatggct
    25441 tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt
    25501 tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac tgagtaagct
    25561 tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt
    25621 tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg ggttcaataa
    25681 agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca agcagcaatt
    25741 atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt gcagtggcta
    25801 tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac gagaaaaaat
    25861 aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg tagatgcaga
    25921 agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag atatatacgt
    25981 cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa
    26041 ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga
    26101 agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg ggatgatggt
    26161 cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc aaaaactact
    26221 tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct cgataccatc
    26281 gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa aagaactgtt
    26341 tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat
    26401 tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg cagcaaagga
    26461 aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct ggcaagggat
    26521 cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata cagcatttgt
    26581 ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg ttccgattat
    26641 ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt acggactgac
    26701 cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta tttcaggcgg
    26761 aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg ctcgattcag
    26821 tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga cactgctaag
    26881 tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt tttatacagg
    26941 aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcacaa
    27001 gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact taattagtca
    27061 aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt tttcagggat
    27121 cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga caacaagtca
    27181 acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc aaggtttgta
    27241 tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt cagttgtaca
    27301 aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt tttcaggtat
    27361 tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac ctgaacatgc
    27421 tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag gtttcgttat
    27481 ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat gaacgccatt
    27541 gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc gttagaacaa
    27601 gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt tgaaacagaa
    27661 catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg tcagctaagg
    27721 aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttaaga
    27781 cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga acaaatccag
    27841 tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt cattgctgga
    27901 gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa tgaacttccc
    27961 tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat
    28021 caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg
    28081 agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg
    28141 aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac
    28201 ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca
    28261 gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga
    28321 gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata
    28381 cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg acaaatcaaa
    28441 atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca
    28501 ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata cgagaagtac
    28561 gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt
    28621 ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag
    28681 tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa
    28741 gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat
    28801 ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt
    28861 atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta aagcttgaac
    28921 agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac
    28981 cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac
    29041 tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg gtgagacaag
    29101 tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg
    29161 tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa
    29221 atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca
    29281 gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat
    29341 caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt
    29401 caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa
    29461 gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt
    29521 ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct
    29581 attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt
    29641 gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa
    29701 cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa
    29761 ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa
    29821 acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa
    29881 atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact
    29941 attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt
    30001 caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc
    30061 atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca
    30121 gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg
    30181 gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat
    30241 gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt
    30301 acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat
    30361 acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca
    30421 gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg
    30481 cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga
    30541 tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt
    30601 gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc
    30661 gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc
    30721 gattggatac aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc
    30781 cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag
    30841 cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg
    30901 acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat
    30961 gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt
    31021 ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt
    31081 accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa
    31141 aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga
    31201 ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc
    31261 ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg
    31321 cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt
    31381 cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt
    31441 agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa
    31501 agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa
    31561 tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca
    31621 tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca
    31681 taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt
    31741 cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga
    31801 ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc
    31861 tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta
    31921 tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt
    31981 gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct
    32041 ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc
    32101 ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga
    32161 cttgactggt tatattacag aaggacagat catcttgact agagagcttt acaagagtgg
    32221 gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg
    32281 acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc
    32341 acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt
    32401 agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt
    32461 ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt
    32521 gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg
    32581 gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt
    32641 gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga
    32701 actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga
    32761 agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc
    32821 tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa
    32881 aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga
    32941 cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg
    33001 gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact
    33061 gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat
    33121 ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc
    33181 agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt
    33241 ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct
    33301 gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc
    33361 ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca
    33421 acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt
    33481 gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt
    33541 ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta
    33601 aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta
    33661 aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt atttgtgcct
    33721 gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt
    33781 aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg
    33841 atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat
    33901 ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt
    33961 acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca
    34021 acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca
    34081 cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt
    34141 ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa
    34201 acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga
    34261 gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt
    34321 tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata
    34381 cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta
    34441 ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg
    34501 cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca
    34561 caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat
    34621 cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc
    34681 ttggatactt taagtgcaaa atgttcgttt attttttga
//
LOCUS       DACDHJ010000008        31495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDHJ010000008 DACDHJ010000000
VERSION     DACDHJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.008"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..548
                     /locus_tag="IV223_000324"
     CDS             15..548
                     /locus_tag="IV223_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8710635.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1038..1562)
                     /locus_tag="IV223_000325"
     CDS             complement(1038..1562)
                     /locus_tag="IV223_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8710636.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1555..2028)
                     /gene="tsaE"
                     /locus_tag="IV223_000326"
     CDS             complement(1555..2028)
                     /gene="tsaE"
                     /locus_tag="IV223_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8710637.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2151..3131)
                     /gene="pta"
                     /locus_tag="IV223_000327"
     CDS             complement(2151..3131)
                     /gene="pta"
                     /locus_tag="IV223_000327"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8710638.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3284..3964)
                     /locus_tag="IV223_000328"
     CDS             complement(3284..3964)
                     /locus_tag="IV223_000328"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8710639.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4090..4959
                     /locus_tag="IV223_000329"
     CDS             4090..4959
                     /locus_tag="IV223_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8710640.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4985..5500
                     /locus_tag="IV223_000330"
     CDS             4985..5500
                     /locus_tag="IV223_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710641.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5547..6110)
                     /locus_tag="IV223_000331"
     CDS             complement(5547..6110)
                     /locus_tag="IV223_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8710642.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6165..6344)
                     /locus_tag="IV223_000332"
     CDS             complement(6165..6344)
                     /locus_tag="IV223_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710643.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6421..6930)
                     /locus_tag="IV223_000333"
     CDS             complement(6421..6930)
                     /locus_tag="IV223_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710644.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7026..7406)
                     /locus_tag="IV223_000334"
     CDS             complement(7026..7406)
                     /locus_tag="IV223_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8710645.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            7736..8485
                     /locus_tag="IV223_000335"
     CDS             7736..8485
                     /locus_tag="IV223_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8710646.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(8669..9946)
                     /locus_tag="IV223_000336"
     CDS             complement(8669..9946)
                     /locus_tag="IV223_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710647.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10025..10669)
                     /gene="pcp"
                     /locus_tag="IV223_000337"
     CDS             complement(10025..10669)
                     /gene="pcp"
                     /locus_tag="IV223_000337"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8710648.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(10868..11812)
                     /locus_tag="IV223_000338"
     CDS             complement(10868..11812)
                     /locus_tag="IV223_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8710649.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(11814..12500)
                     /locus_tag="IV223_000339"
     CDS             complement(11814..12500)
                     /locus_tag="IV223_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8710650.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12974..13294
                     /locus_tag="IV223_000340"
     CDS             12974..13294
                     /locus_tag="IV223_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8710651.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            13448..14353
                     /locus_tag="IV223_000341"
     CDS             13448..14353
                     /locus_tag="IV223_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8710652.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14350..15123
                     /locus_tag="IV223_000342"
     CDS             14350..15123
                     /locus_tag="IV223_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8710653.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15304..16728)
                     /locus_tag="IV223_000343"
     CDS             complement(15304..16728)
                     /locus_tag="IV223_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8710654.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16939..17385
                     /gene="nrdI"
                     /locus_tag="IV223_000344"
     CDS             16939..17385
                     /gene="nrdI"
                     /locus_tag="IV223_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8710655.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(17450..19432)
                     /locus_tag="IV223_000345"
     CDS             complement(17450..19432)
                     /locus_tag="IV223_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8710656.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(19446..20342)
                     /gene="murQ"
                     /locus_tag="IV223_000346"
     CDS             complement(19446..20342)
                     /gene="murQ"
                     /locus_tag="IV223_000346"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8710657.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(20345..21406)
                     /locus_tag="IV223_000347"
     CDS             complement(20345..21406)
                     /locus_tag="IV223_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8710658.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(21617..22144)
                     /locus_tag="IV223_000348"
     CDS             complement(21617..22144)
                     /locus_tag="IV223_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8710659.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(22211..23713)
                     /locus_tag="IV223_000349"
     CDS             complement(22211..23713)
                     /locus_tag="IV223_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8710660.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23971..25092)
                     /locus_tag="IV223_000350"
     CDS             complement(23971..25092)
                     /locus_tag="IV223_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8710661.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25218..26159
                     /locus_tag="IV223_000351"
     CDS             25218..26159
                     /locus_tag="IV223_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8710662.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26309..27205)
                     /locus_tag="IV223_000352"
     CDS             complement(26309..27205)
                     /locus_tag="IV223_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8710663.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTSFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            27474..28814
                     /locus_tag="IV223_000353"
     CDS             27474..28814
                     /locus_tag="IV223_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8710664.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            29032..29475
                     /locus_tag="IV223_000354"
     CDS             29032..29475
                     /locus_tag="IV223_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8710665.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29979..31397)
                     /gene="nhaC"
                     /locus_tag="IV223_000355"
     CDS             complement(29979..31397)
                     /gene="nhaC"
                     /locus_tag="IV223_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8710666.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
BASE COUNT         9151 a         6520 c         5664 g        10160 t
ORIGIN      
        1 gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat catcagcctt
       61 atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt gatgaattct
      121 atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt cacggaatcc
      181 atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact atccaaccat
      241 attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca aaggtcttag
      301 cgggatgctt agattattat catcttgaac agcctaatta tctttcttta tgtacagtga
      361 aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact gtgtgcgaaa
      421 atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt gcatgtgcgg
      481 aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa cttgtgacta
      541 taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg tttcactccg
      601 agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt gtgactggtg
      661 accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag ctgttctgca
      721 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttggactt
      781 attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa tcagcgtttt
      841 gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc gttttttgag
      901 ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga ctgcgacacg
      961 acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga cagtttctca
     1021 ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc taaaaattca
     1081 cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc catttttcgg
     1141 taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg aaaaagtaca
     1201 cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag tcccattccc
     1261 caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg aaattgactg
     1321 tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag cagcaataaa
     1381 ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg tggtaacagg
     1441 agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc catcatcaaa
     1501 agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac ttcttcactc
     1561 atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt gctccccgta
     1621 tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta aaatcagttc
     1681 caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa cggaaagccc
     1741 atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta cccgataaat
     1801 atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg tgtatgtcgg
     1861 acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca acgttgtttt
     1921 acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg aatgaccaat
     1981 tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt ttcatcttct
     2041 cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa agaaaaaatg
     2101 aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg ttaatcaatc
     2161 aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt tgctccacga
     2221 gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc ttcaaagtta
     2281 ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg aaatacaaag
     2341 actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac tgaaggaaca
     2401 taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag ttttttagcg
     2461 attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc ttttgtcgag
     2521 aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt tgcagaatct
     2581 acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca atccgaaaag
     2641 acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt ccgactgatg
     2701 ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt cgaatggatc
     2761 gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc aaagtaattc
     2821 acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct acgttcaaca
     2881 aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat cgtaaagcta
     2941 gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt ccctactaaa
     3001 atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat acgagcatca
     3061 gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct taaactatca
     3121 aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca ctattccaga
     3181 aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat taagtttttc
     3241 acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact gtttcaggca
     3301 gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt ttcgagaagg
     3361 gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga gatgttatga
     3421 tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt ccccacaaga
     3481 taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct gttaatcttt
     3541 cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga acggtgttga
     3601 gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca ggtgcgattc
     3661 caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc tttacgccag
     3721 gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga tcttgtccta
     3781 aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag atatgataca
     3841 tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt agatgcagat
     3901 aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga atgatttctt
     3961 tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc acaagggaca
     4021 atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca gaaaggaacg
     4081 ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt agatgcccat
     4141 atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc cggtgttgag
     4201 ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga tgaggtagga
     4261 atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga aggaaattca
     4321 ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat tctagatgct
     4381 aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa tcaagcgaaa
     4441 cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac gtataaaatg
     4501 gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt ggacagtatt
     4561 cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt tcatacagaa
     4621 gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga tctagccgtt
     4681 acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa aggaattgct
     4741 gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac gattggcgat
     4801 aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat gggaaatgca
     4861 gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga atctggcgta
     4921 ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga aaagagggat
     4981 atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc ggacaattgt
     5041 caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa caaagggcga
     5101 tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc agataagcct
     5161 gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa ccatgcagaa
     5221 aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga cagtccaagg
     5281 tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca tgaaaatgga
     5341 taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg atgaagatgc
     5401 tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta aagacaaaag
     5461 tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac ctgaaatgcg
     5521 gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc ataaagatga
     5581 ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa ttttttagcc
     5641 tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt cttcgccaat
     5701 ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc ttctgataca
     5761 ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa caaacgtgta
     5821 gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat gattgattct
     5881 tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc ttctacgata
     5941 cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa aatctctaaa
     6001 gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag aatcactaag
     6061 atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat agtttcctct
     6121 ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt taaaaaatct
     6181 tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc agttcggcaa
     6241 taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt gacttcttcg
     6301 tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc tcaacttcct
     6361 ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa tatccttctt
     6421 ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga ctttataata
     6481 aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt tcccgtagta
     6541 gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt tttctatatc
     6601 aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc cagtggatgg
     6661 attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg cattcgtagg
     6721 attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt cttttgtttc
     6781 gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt cactaatatc
     6841 atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta ttacgatgag
     6901 ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt ttttttaaag
     6961 aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg acaattttgt
     7021 tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa cgtcattttt
     7081 tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga ctgcaattct
     7141 gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc acaagcttct
     7201 ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc agcacttcct
     7261 agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct gattacttca
     7321 aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac aatgatggct
     7381 atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta agctcggatc
     7441 tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc tgtttttcct
     7501 ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac tcttcctgtt
     7561 cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca gaaaattatc
     7621 ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc atacaataga
     7681 ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct catacatgaa
     7741 gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa attattatct
     7801 tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca tcacttggct
     7861 gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat ccatcaatgc
     7921 ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta ttatcgttgg
     7981 gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga tcacattgga
     8041 tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa tccgaaaatt
     8101 cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc ttgtttcagg
     8161 attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca ttgctttttc
     8221 tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat attactggtg
     8281 gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc atggatctgc
     8341 aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc ttgcatttac
     8401 cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg aagaagaata
     8461 ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta aagaacactt
     8521 ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct tcggaaataa
     8581 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
     8641 taaacgcttc tgtcccagcc tctatttttt atagtaattc cttcagatac tttcttgcct
     8701 catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag
     8761 cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag
     8821 attggatatc tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat
     8881 cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta
     8941 tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa
     9001 taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag
     9061 cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa
     9121 aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta
     9181 aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt
     9241 cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta
     9301 ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt
     9361 ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc
     9421 gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat
     9481 tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat
     9541 ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta
     9601 gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac
     9661 cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga
     9721 agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga
     9781 ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga
     9841 tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga
     9901 tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc
     9961 tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc
    10021 taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt
    10081 caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg
    10141 gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct cgaattgccg
    10201 ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc
    10261 ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt
    10321 tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg
    10381 gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga
    10441 acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct
    10501 gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt
    10561 tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat
    10621 tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct
    10681 ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta
    10741 aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa
    10801 aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc
    10861 tcttctatta tttaaataga atcatataag caatttggaa aactagcata aataacgcaa
    10921 taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca
    10981 cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg
    11041 caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc
    11101 caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca
    11161 tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta
    11221 ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta
    11281 aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta
    11341 aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa
    11401 caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa
    11461 agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt
    11521 ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg
    11581 gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg
    11641 caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt
    11701 tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga
    11761 tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc
    11821 ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt
    11881 gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt
    11941 acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg
    12001 aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt
    12061 tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc
    12121 ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca
    12181 aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat
    12241 cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc
    12301 aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt
    12361 tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt
    12421 acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa
    12481 aggctaagat aattttccat atattttcct ccataaaatg acaatatttt tttaattata
    12541 aagcaaaaca cataaaaaat atagttgttt tctcacgaaa tagacactat ttttaaatat
    12601 ttttcgttat ttttttatca caaaaattat cttacttatt gtaaggtaaa gcttttttta
    12661 atttattgcc cgataatata tagaaataag gtctagttac tgtttttttt aaatgattaa
    12721 taaataacaa cttctttcta tgaaattgca aagcattcat cttcttttcc gctaacgata
    12781 tatccgtcag tgcttggtac actttactaa aaagtatgag attgccgata tctggatagg
    12841 tgtgctatac tacagaaaaa gatatacaat ctgccaccgg gtaggataaa gaggatgatc
    12901 attccattag gtctttgaag ggatgatttt cctctttttg tgtatttttt tatagttaag
    12961 gaggaatgta agtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
    13021 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
    13081 gattggcatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgccgatgag
    13141 tctctcttat cctatctgga cagggattgg cgcagttggt tcggtcataa ttggtgtggt
    13201 cttgtttcat gataagctga atctttccac ttggttcttt gtggggatgc tactcatcag
    13261 tatcattggc atcaagatta ctagcggcca ctagacactt ggaataagta ctgatcagaa
    13321 aaaataagcc tttttattta atattcaatt tttttatgaa aaaataagaa ataagtccaa
    13381 tgacctagcg acttctccac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg
    13441 aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc
    13501 cctcagcgat gtcagtttta gcttatcccc tggcaaaatc gttggattag taggtcctaa
    13561 tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagatgg
    13621 aacaatctgt attggtgaaa atgagatttc tccctctagt cataaagggt tggaacaagt
    13681 aggagcattg atcgaatacc cgggtatcta tccattctta acaggctatg atcatctgaa
    13741 gttattctct gaaaccaacg atgtatctgc tatcgacact atcgtgaatc agctcaagat
    13801 ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac aaaaattggg
    13861 aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg
    13921 tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg
    13981 cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct
    14041 gatcctgatc aatcaaggaa agatcatcaa acaatgtaca atggctgaat tgaaccaaac
    14101 agcgcaagat ttcatcgtta tctctacaac aaatgatcta gctgctcgaa cgatcttgac
    14161 agaagctgga tatactttgg aagatcctga taaaatcaag cttttaaaag aaaacgaaga
    14221 attattagga accgtgatcc agctattgtc tgcacaaaat atccatgtga cagacgtcca
    14281 gcatctgacg aatgatttgg aatcttctct attggaaatt ctggattcag taaaggggga
    14341 agaagaaaaa tgacattgct tgtaaaacaa gaattattta aattgatcaa gaaaaagtca
    14401 actgctgttt tatctgttct attagttgtt ttgctcatag gaacagcact tctagccaaa
    14461 aaatacacga cgatcattga tccagttgaa atgactgctc agctttttag tgccacatct
    14521 tggattgtct ttatcatgat tgcagctgca agcacgatca tttcgatgga agcacaatat
    14581 ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagcaaa
    14641 tggatcacat tagttattta ttctgtttat ttataccttt tagctattat tgtcactgtg
    14701 ttgatgaaac tgattctttt cccttcgatt tccttcacgg agaaggtttc tactggtcaa
    14761 acactgatcc aaagcttgtt cacttatact ttgggtagtt atatcggttt atggctgatt
    14821 ttaagtttag tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc catctcagca
    14881 ggaatcgtct tttattttgc ttcgtcaatc atatctggta tccttattgc actcatccaa
    14941 aaatgggagt ggatcaaatg gaatccgatc agtatgttga atctgcaaaa ccaaattgga
    15001 aacgaagaga tcatgaagca attgactcat ctttctacta atcaaatgct attcggtaat
    15061 ttagcttaca tcgttttgtt cttagcatta ggatatctcg tatttaagcg aaaaaatgta
    15121 tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc cgattggctg
    15181 tctcttaaac accgtttatt ctgaaaaagg agctgtgaca acatttgtgt cacagctcct
    15241 ttcctagtat gtttttgaat aaacgacagg ctcacaacct ctctttatac ttcttttata
    15301 cttctatacg tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc cgaccatcgt
    15361 cacacctata agtaaaatct tatctaagtg gatatgttgt acaggctcgt tttgctcctt
    15421 acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa gcattacttc
    15481 attaaaggct acgactagca tagcccatac ttgaaaggca cttgctaaaa gccctactaa
    15541 aaattggacc cattctttcc gctgataaga aagtttcatt tggtacaatc ctaagaaaag
    15601 ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca aagttataag cactgtcagt
    15661 gaataataat gaaagtaaaa agacattcgt acataacgtc gtcaatacga tagcataagt
    15721 tggtgcccca tttttgttga gttttcccca tgctttaggc aaaagtccgt cttcagccat
    15781 gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa ctaaagaaat
    15841 gatcaaaccg ccgttgatca cgactgctcc ccatttacca accatcattt caaacacata
    15901 gcccatagca ggctgcgcct tgatcgtttg cagctgattc atcgtcattg tcccatatgg
    15961 aagcatggaa accaagacgt aaataataat caagccgaat atccctagaa tcgttgcttt
    16021 cccaacatct gattgttttt ttgctcgtga acctacgaca gaggctcctt caataccaac
    16081 aaatacccaa agcatcacca tgatgcaact caatacttga ctggaaaacg ggatcggctg
    16141 tccattcgca ttcaatataa tgtcgctaga aaagttatca atgaattgtt ggatcgaaaa
    16201 cgtgctgata gctactacaa taaataaaaa taatggcact aatttacaga atgtgacgat
    16261 agcattgacg aaactagcat tttcgactcc ccgattcact agccatgcat atccccataa
    16321 aaagactgta ctcatcacga tcgatgccag attttgccca ccttcaaata ctgggaagaa
    16381 atatcctaat gcgctcatca acaatgttgc aaaagctacg ttgcctaacc agcatgtgag
    16441 ccagtaaccc caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact
    16501 aaagatcccg ccttttaaat ctggacgttt tttattgata ttattgatcg acaaggagag
    16561 catcaacatt gctgttccta cgagtaccca actgaaaata gctgggccgc cagccgatcc
    16621 ggagatgtcc gtcataatac caaatacgcc acctccaatc gctgaaccaa ttactaaggc
    16681 agcaagtgga attaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc
    16741 caaaataaaa ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa
    16801 taaaaacttt aatagaaata taattctacc ggcttttttc tcattataca agcaaagcgc
    16861 ctagtccctt tccaaattac accaccaaaa aacttaaaaa agatgctata ctaaaaggaa
    16921 agaattggag gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgcc
    16981 tttgccaaac atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct
    17041 gaagttactt taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt
    17101 tttactttcg ttcctactta tttagacggc ggaaatggtt tagataacgg tgatacagaa
    17161 atcttgaccg aaacgatgcg tgaatatctg gaacatgaag acaatcataa actttgtctt
    17221 ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt attgtctaac agccaaacaa
    17281 tatgcacaaa catttggctt tcctttcttg gcagattatg aattacgggg aacaccatct
    17341 gatgtgacac gaatttatca gattttagca gaaaacaata aataataaaa aagaagaagc
    17401 tgaaaaatag atttcagttt cttctttcgt ttctattgtt tctatcatat taagctttga
    17461 catccatcac aggtgctttt gcttttgctt tacctgtttt ttccaagaca aagtttttta
    17521 cacgatccat attggttacc aagacgatca tggatgtttc tttgcctgct gctttgatcg
    17581 cttctaagtc agcttctgct acaagcgtat caggtgtcac ttgctgccct tctgttactt
    17641 tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga agcaagattt
    17701 ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca gagctgacta
    17761 ccccagtaac tggagaatag atatgtccgt tttctggttc tacaaagtag ccttcaccca
    17821 tcatcttttg tgcaaaaact ggatcacttg ctttatctaa agaaacaact gttccgtcag
    17881 caactgatga gaggatagtc acactttcag ctgaagtttg aggtactact ttttcaggca
    17941 cttcatttac tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg tctttaggaa
    18001 taccgaagaa gaatgtagca acgaagccac cagcataagc tgccaataat ccaaggatgt
    18061 agcctaacca tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa ggtccaatag
    18121 cgatagcccc gacatttccg atcattccga tcactgcccc gccgatacct ccaccgatac
    18181 aagcagtaat aaatgggcgt cctagaggta atgttactcc gtaaatcaat ggttcgccga
    18241 ttcctaggat cccgactggt aaagctcctt tgatcatttc tgttaattct ttatcttttt
    18301 tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc atcgcaagaa
    18361 ttggaagcaa tagcgtacga ccagtttcat caatcatttg cacatggatt ggagtcaaaa
    18421 tttgatgcag tccgaacata accatcggca agaataatgt gcctagaacg aagccggcaa
    18481 atgctccacc aacgccaagt acccagttga tcccaccgac catgccatca gaaatgaaac
    18541 ctgctaaagg catgatgaag aaaatttcaa tcagaccgat ggcaatcaat gaaagagttg
    18601 gtgtaacgat gatatctacc gcatcaggta ctactttatg cagttttttc tcaacgattg
    18661 acaatagcca aacagcgaag atgacaccta ggatcccacc ttgtccagct gcaagagctt
    18721 caccagtaaa taagtttttc agtggtgctt ccggattcat ccctgtcagc atcacaacgg
    18781 ccccgataac tccaccaaga gttggtgttg caccaaaaac ttgtgcagca ttgatacctg
    18841 tatagataac taggtagcta aacatcccgt ttttcaagat gttcatcaca tcgatatatt
    18901 gttgccatgt tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa actgcggcaa
    18961 tccctgccac gatcccagta ccgacaaatg caggaatcat tggcacaaag atattagaaa
    19021 tgtcttttaa taccgatttg attttagatg gtttttgttt cgatttttgt gccgctttca
    19081 tttcttgtgc ccgttcgttt acacggtctt ttccagatgc tgatgaattt gctgtagctg
    19141 tacttacaga agaaagtttt tctcctaatt tcacgcctgc caaatcaacc atttgctgtg
    19201 ctactttgtt cactttccca ggtccgatga tgacttgaag ctgttcatct tctactacac
    19261 caagcactcc tggaatcgct tttaattcag agacatttac aagatcatca ttgatgatct
    19321 tcatacgcac acgagtcatg cagtggacaa tgctttctac attgcccatg ccgcctgccc
    19381 ctttataaat ttgttttgcc agttcttcca aagtcagttc gttttttgcc atggtctttt
    19441 ccccctcata aggtcccttt tacaaatccg tttgcttcag ctaattttgc ttgagcttcc
    19501 tctttatcac tatttgtcaa aatcatgacg atagccagct taacattttg atctgcttct
    19561 tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat gattcgtttt
    19621 gagcgttcga ttagtttttc gtttgttggt ttgacatcga ccatcaagtt attgaatact
    19681 ttacctatac caatcatact gatagtagaa atcatattca agataagttt ttgtgctgtt
    19741 cctgacttca atcgagtaga gcctgtcaaa aattctggac ctgcatcgac ttcgattggg
    19801 aaatccgcgt gtttactgat ttcggcttgt ttattgcaag aaatcgttcc tgtgacagca
    19861 ccgatcgatt ttgcgtaatc taatccaccg atcacatatg gtgtacgacc gctagctgcg
    19921 ataccaataa ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc ttgccctagc
    19981 tcataagaat cttcggcccc ttcgacagct acagtcattg cttcttctcc accagcaatt
    20041 agacctatga ccatttccgg ttctacaccg aatgtcggta cgcattcagc agcatctaac
    20101 acacctagtc ttccacttgt tccggctccc atataaatca gacgtccacc ttttttaaag
    20161 gactcaatcg tgttagcaat cactggttcg atacttgtca gttctttttc aaccgcagcg
    20221 gccactttct gatcttcttt gttcattagt tgaacggctt gagaaatact catctcatct
    20281 aatccaaaag tttcttggtt tctgcgttcc gttgttaggt tctctaaatt gattttgctc
    20341 atttttatct tccttctttt cttataaatt gatagttcat accagctttg atttggttga
    20401 tcaatggtag ctcttctttg ataactttag ctactacatt cactttttca tccgctggca
    20461 aatcatattt ggttaattgg atctcaccca tatagcgcaa atacttttcg ttatctaaag
    20521 taatagaccc tttcatacgc gtagctgtat ttcttgctgg gataggcgga atctctcgaa
    20581 aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc gtatgcttcc
    20641 ctaatatata ttcgtaaaat tcttcatcta caggttccgt atggagcaag actgtctgtt
    20701 ctttttggaa agaagcaaat tgctcctgaa cgttttctga cagacctgca tctccgatgt
    20761 aaacaatatc cgtgttactt gctaatagat ctagagcgcc tgcaagagga tggatccctc
    20821 gatgttcttc caaagttggc aatccttgat atagtgggcc tctcaactct gtatctcctg
    20881 gtacaaaacc ttggacaagg agaccttgaa ttttgagcca ttgatttttt ttctgatacc
    20941 attctttgtc cagccctgtt tcgggacgtg gataataatt atgccaagct tcaatctgtg
    21001 agaaatcagc ttctgcttct ttcaattcat cgatatcttt aggcgtgatc gtgctagcat
    21061 tcaaactgat tttcaattga tgtgaccagt cggcaatctg ctgattggaa atatgataat
    21121 ccattcttaa acctgttaca ccaattgctt tcaaaggcgt tagatcttct aatgaaaaac
    21181 ccgctcgttc caatgcctct cctgaaatat cgaccatcaa ttctagttta ttctttttcg
    21241 cttcgcttcc tagttcgatc aatcgctttt gatatgccga aacatcatct tctggtatat
    21301 gcatggaagt gaaaataccg acaaagccat tggatgccat ttggcggata tactgtttct
    21361 tctcttctga caaagaatcg ttcataaata ttgaaaagcc aaacatatta tccctctttt
    21421 tctgtatccg tcttcatttc tatgactaga gtatatatct aaagaaacaa aatttcaata
    21481 cttttattaa aataattaaa tttcatttct aaaggttgag tttatattat caaaagaatt
    21541 gtcataacct aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat tttatttaaa
    21601 taacacaaaa acataattac tcataagaaa ccaattctgt attatctacc atgaatgtaa
    21661 agaacccctc attaggcatc ggatgaaaat aagcattgat cactcttatc aaggcattat
    21721 ggcaaactaa cagatagaca ttttcttcgt cttcgatgca ttctttcaat aaaggaacga
    21781 tccgtgcata cacatctaag actgactccc catttgggaa tcgtacagcg aacgctaaac
    21841 gtgctttttg aaagttctca tctttgcttg gcagtccatc ataatcccca aaatccattt
    21901 ctaccaatcg ttcatcaacg gtcagcggca agctgagacg atcagcaact gcttgtgctg
    21961 tatctcttgc tcgttgaaga ggggaatgga tgattttcgt gattggaaca ggcaaatcta
    22021 ctaccttttc tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg
    22081 cccgtccgca aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa
    22141 gcatgtgatt ctctccttcg ttggcaaagt tccccttaac cataccttgt tgttcttgag
    22201 attacaaggg ttagattctc tcaagagaaa taatctcagt agatacttta tccaggaagg
    22261 tttgatcatg ttccactagt aacatggcag gtttcacgtt caagatcagt tgttctaatt
    22321 gttcttgatt gaagacatcc aaataattca atggttcatc ccatgtatat agttcagctg
    22381 gctgtgataa agatttagcc aattccactt ttttccgttg gcccatactc atctgctcga
    22441 tcttgttatg aaaaacatct ctttccatcc caagctttcg gaggttgttc aaaaatgctt
    22501 ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg tcttcataat
    22561 tttggcttgc ataactaatg ctcagatgtt ttggctggta tttttctcct ataaccttgc
    22621 cgttgaatgc ccccagaaga taatggataa tggatgactt tcctgcaccg ttaggacctg
    22681 aaatcgccac ccgctgatga ggctcgattg taaaatgaat tggctcgaat aacagatttt
    22741 cataccctaa ttgaagatct tctacgctta aaagtcgctt atggtgagac gcttggctat
    22801 tcatcgtcaa agaatcgata tattcgatat cttttaatag tttttctttc tcgctgatct
    22861 gggtctccat ccgattcacg attgctttgg atcgtttcat cgtccgtgca gcatcagcac
    22921 ccactgctcc tttattcact cgtctagatt cagtatcgat gaatccgact tgtttctttg
    22981 ttttatctcc ttctcgggaa cgagaccatt cggctttttc agctgctgtt ttctttagcc
    23041 tactgacttc ttttttcaat ttttcatttt gagccatttc aaattcatca cgaagttttt
    23101 tctgttcttc atagatagag aaattccctt gataaagttc cagttgactt ttttcgattg
    23161 ctaaaacatg gtccactact tcatcgataa atccccggtc atggctgacc acgatgaagc
    23221 cttgtttttt ctttttcaaa taagccgcta cttgttttct accagagata tccaaatgat
    23281 tcgttggttc atcgattaac gggaaatgag tgtcatccac aaataaaagt gccagtagga
    23341 cttttgtctt ctctcccccc gatagtgtgc tgaattctct ccataagatt tcaggatctg
    23401 tttgcatcaa ttggagctct ctttcgattt cccatatctc aaaatccgta atatcattta
    23461 acacgtaata ggttaaacgt tctttatctt ttgtctgttg cgggaaatag gcaaattctt
    23521 gctgatggat tacttgccct tgataaggta gtttgttttg tagaatattc agtaaagttg
    23581 tctttcctcg accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca aaatttagat
    23641 ttgcttgttc aaataataat gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt
    23701 cgattttaga cattttccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc
    23761 taaagaggat gcaagggttg gatacacaca aaaagaccca tacatcgaaa aaaccaagcc
    23821 aatcccgaaa atctgcacac tgcttccttg tctccacaag gtacagtctg aatgcagctt
    23881 tcatcaatcg acttaatttt tcaatgcacg gatccctcac tttgtcctat atttgaaatt
    23941 acgatagcat aaagaaaatg attttgtcaa ctacttcggg aggatggtga aaaatctgct
    24001 aagtgtctgt cctaagattt ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc
    24061 taaacctatt ggacccaaat gaatacttaa gacaattggg agaaaatcag aaagtccttc
    24121 tagctcttgg tctctacttc cgcaaatagc gattattggt tttcgatatt tttttgcaag
    24181 cactgctaca ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc
    24241 tgtaatgatg acatctgctt gttcgatctt tttttccaat ccagtgattt ggctgactaa
    24301 atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc
    24361 tgctccactt cctggaatat catctaaatc cacctcccag aaatctttta tttgctgagc
    24421 aatctttcct gcttgtttat ctaagaaagc tagcgtcgta tctattcctc ctttttgtct
    24481 tccaaattgc caagcagctc cttgttgacc tgtatatgga ttggtaacat ctgcagcgat
    24541 aatcaatttt actttagaaa caagaacgtt tcttcgatca aaatgaagtt ttttagtaga
    24601 taataaagga tttccttctg tgtacccttc taaagaagca cccaaggatt ggagtaatcc
    24661 tagaccacca tcgctacacc cgcttcctcc taaagtgaca ataatttgtt ctacttcttg
    24721 aagcagtgca tctttaataa gttctcctaa ggcataactg gaagcaagtc ggatagtttg
    24781 atccgttggt ttatcaagta gatctaaccc caatgcttgt gcagattcga tgactgcggt
    24841 gttttttcct tgccaagttg tcaataaata gctgattttt cgctgacgga ataagagatc
    24901 catcccatca atggtacgcc atgttcccct gagcgcgtaa tagattgctt ctgcagtatt
    24961 ttctccgcca tctgcaatcg gcacattgat ttttcttcct ttacgatctt cccagccatt
    25021 tagaactgcc tgatttagtt caaatgaaga agcacttcct ttaaatgaat caattgccgc
    25081 caaaatatcc atctgtatcc ctcactttct tcttaagtaa gacacttttt ttcatttttt
    25141 tcaaacaaaa caatgctatt ttcttccatt tttctatata ctaaaaggga actcattgaa
    25201 taaaggagag aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa
    25261 tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt tggcctgctc
    25321 acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa gaagtggtcg
    25381 caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac gtggacttga
    25441 tcgtagcatt aacaaaagcc aatcaattag atgatatgtt ttgttcgatc caatccatta
    25501 ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa gatgtactgg
    25561 aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag
    25621 ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag aacttggaac
    25681 cagaaggcga agcatttaca aaaaaagttg tagaagtctt tcaagaagca aatttaaatc
    25741 cgatctatag ccataacgtt cgttattcca tttggcgaaa agcttgtgta aatggaacat
    25801 tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg caaaaagcgg
    25861 cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca aaagaaggaa
    25921 tcatactaga tcaagaagaa gtgtaccaac atattgcttc tacttatgat cctgacaaca
    25981 tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa
    26041 tcgactacat caatggtgcc atttggcgaa aaggacaaaa atatgatatt gctacaccat
    26101 attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa
    26161 aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta agcccaataa
    26221 aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg tttgtttcag
    26281 tacataatct cttcaaaatg taggtttttt atttgttttc aacagaggag aggtcatatt
    26341 cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa ttttctgcat
    26401 cataaaattc caatagctct gataggattg gcttaaatga tgtatgcaga aattccggta
    26461 attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa aaatgataat
    26521 gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatggat tcaaatgtcc
    26581 gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc gattcccctg
    26641 tttttttcag gtactcttca taagtaagat atccaggttg ttcattccct cgatgtggaa
    26701 gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag ctttctgctc
    26761 gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt tcgctagaag
    26821 aaaaattcat atgtttcgaa taaaatgaac tattatgttt cagcccaagt tcttttgctt
    26881 ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca
    26941 tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt gcattggtaa
    27001 atgggcgcac gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct
    27061 gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt aaatcttttc
    27121 cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta ttttttgcaa
    27181 ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca ctttttccta
    27241 tgatagcctg tttaaatagt aaaaagccag taaaacgttt cctatatttg tatgaaaatt
    27301 taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt cgcctaaaag
    27361 ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt taaatattgc
    27421 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
    27481 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
    27541 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
    27601 tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa
    27661 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
    27721 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
    27781 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
    27841 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
    27901 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
    27961 tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
    28021 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
    28081 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
    28141 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa
    28201 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
    28261 tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg
    28321 cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg
    28381 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
    28441 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
    28501 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
    28561 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
    28621 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
    28681 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
    28741 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
    28801 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta
    28861 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
    28921 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
    28981 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
    29041 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
    29101 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
    29161 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
    29221 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
    29281 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
    29341 cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
    29401 ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
    29461 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc
    29521 agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa
    29581 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
    29641 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa
    29701 atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct catattttgc
    29761 cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga gaggttgtga
    29821 aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc cacatttttg
    29881 tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc gtaaaaggga
    29941 ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa ttgcgctgtc
    30001 ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc tgtgaaggca
    30061 ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt
    30121 gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg cactaagtag
    30181 ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc aaatcctgct
    30241 ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa ttgttcacca
    30301 ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc
    30361 gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc gatcaacaat
    30421 ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact ttcgattcct
    30481 ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa ccctgattcg
    30541 atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat cattcctact
    30601 gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca tgcacacagt
    30661 aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc ttctagaaca
    30721 tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg
    30781 aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg cttgatgtgt
    30841 ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga taatggagac
    30901 atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc tggatttaag
    30961 cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca
    31021 ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac aaaccactgg
    31081 acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc ttgtatccat
    31141 acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc tggtttgatg
    31201 ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac tgcatacaac
    31261 atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa tccgacaacg
    31321 ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa aaaacttggt
    31381 gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt
    31441 taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata aaaat
//
LOCUS       DACDHJ010000009        31045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDHJ010000009 DACDHJ010000000
VERSION     DACDHJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.009"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..940)
                     /locus_tag="IV223_000356"
     CDS             complement(65..940)
                     /locus_tag="IV223_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8710667.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1049..2818)
                     /gene="aspS"
                     /locus_tag="IV223_000357"
     CDS             complement(1049..2818)
                     /gene="aspS"
                     /locus_tag="IV223_000357"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8710668.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2833..4125)
                     /locus_tag="IV223_000358"
     CDS             complement(2833..4125)
                     /locus_tag="IV223_000358"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8710669.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4519..5466
                     /locus_tag="IV223_000359"
     CDS             4519..5466
                     /locus_tag="IV223_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8710670.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5555..6814)
                     /locus_tag="IV223_000360"
     CDS             complement(5555..6814)
                     /locus_tag="IV223_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8710671.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(6985..7431)
                     /locus_tag="IV223_000361"
     CDS             complement(6985..7431)
                     /locus_tag="IV223_000361"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8710672.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7442..9655)
                     /locus_tag="IV223_000362"
     CDS             complement(7442..9655)
                     /locus_tag="IV223_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8710673.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9888..10652)
                     /locus_tag="IV223_000363"
     CDS             complement(9888..10652)
                     /locus_tag="IV223_000363"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8710674.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10653..11600)
                     /gene="prmA"
                     /locus_tag="IV223_000364"
     CDS             complement(10653..11600)
                     /gene="prmA"
                     /locus_tag="IV223_000364"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8710675.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11613..12101)
                     /locus_tag="IV223_000365"
     CDS             complement(11613..12101)
                     /locus_tag="IV223_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8710676.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12067..12738)
                     /locus_tag="IV223_000366"
     CDS             complement(12067..12738)
                     /locus_tag="IV223_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8710677.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12876..14162
                     /locus_tag="IV223_000367"
     CDS             12876..14162
                     /locus_tag="IV223_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8710678.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14234..14428
                     /gene="ssrS"
                     /locus_tag="IV223_000368"
     ncRNA           14234..14428
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV223_000368"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14506..14790
                     /locus_tag="IV223_000369"
     CDS             14506..14790
                     /locus_tag="IV223_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710679.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14863..15342
                     /locus_tag="IV223_000370"
     CDS             14863..15342
                     /locus_tag="IV223_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8710680.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15565..15708
                     /locus_tag="IV223_000371"
     CDS             15565..15708
                     /locus_tag="IV223_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8710681.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16286..17869
                     /locus_tag="IV223_000372"
     CDS             16286..17869
                     /locus_tag="IV223_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8710682.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18306..19490)
                     /locus_tag="IV223_000373"
     CDS             complement(18306..19490)
                     /locus_tag="IV223_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8710683.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19515..20522)
                     /locus_tag="IV223_000374"
     CDS             complement(19515..20522)
                     /locus_tag="IV223_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8710684.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20694..21053)
                     /locus_tag="IV223_000375"
     CDS             complement(20694..21053)
                     /locus_tag="IV223_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710685.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21025..21396)
                     /locus_tag="IV223_000376"
     CDS             complement(21025..21396)
                     /locus_tag="IV223_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8710686.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21413..21715)
                     /locus_tag="IV223_000377"
     CDS             complement(21413..21715)
                     /locus_tag="IV223_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710687.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21732..22202)
                     /locus_tag="IV223_000378"
     CDS             complement(21732..22202)
                     /locus_tag="IV223_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8710688.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22177..22455)
                     /locus_tag="IV223_000379"
     CDS             complement(22177..22455)
                     /locus_tag="IV223_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8710689.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22452..23501)
                     /locus_tag="IV223_000380"
     CDS             complement(22452..23501)
                     /locus_tag="IV223_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8710690.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23458..24387)
                     /gene="cpaF"
                     /locus_tag="IV223_000381"
     CDS             complement(23458..24387)
                     /gene="cpaF"
                     /locus_tag="IV223_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8710691.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24519..26273
                     /gene="ade"
                     /locus_tag="IV223_000382"
     CDS             24519..26273
                     /gene="ade"
                     /locus_tag="IV223_000382"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8710692.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26371..27033)
                     /gene="trmB"
                     /locus_tag="IV223_000383"
     CDS             complement(26371..27033)
                     /gene="trmB"
                     /locus_tag="IV223_000383"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8710693.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27103..27882)
                     /locus_tag="IV223_000384"
     CDS             complement(27103..27882)
                     /locus_tag="IV223_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8710694.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28089..29300)
                     /locus_tag="IV223_000385"
     CDS             complement(28089..29300)
                     /locus_tag="IV223_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710695.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29293..30030)
                     /locus_tag="IV223_000386"
     CDS             complement(29293..30030)
                     /locus_tag="IV223_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710696.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30168..30602
                     /locus_tag="IV223_000387"
     CDS             30168..30602
                     /locus_tag="IV223_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8710697.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30595..30939
                     /locus_tag="IV223_000388"
     CDS             30595..30939
                     /locus_tag="IV223_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8710698.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         9156 a         6569 c         5542 g         9778 t
ORIGIN      
        1 aaaaactgca gacaaacgaa cgtctgtcta cagcagtatt tcatgaattt ttgtcaaact
       61 cgatctaaat gccatcctca tagaaacgac caagtatctg gacgttttct gcaatactta
      121 caaaagcttg tggatcagaa gctttcattg cttctctaag tagaggcagt tcgtatcgtg
      181 taacgatcgt catcaatact gtctgcttat catgcttata agcaccttct gcttcattga
      241 tgatcgtgat ccctctacgc atacagtttt ggatttcatt tactactttt tctggatgtt
      301 tcgttacgat cattacctgc atcttctttt gtttagtata aacgacatca gttacttttc
      361 cactgacgaa aatagacaac gtactgtaaa acatatattg ccagccaaat agaattccgg
      421 caacaaaaac gatcaatgca ttgaagtaga tcgaaataga accaatcgac tttcctgttt
      481 ttttacgaat cgtgatactc acgatatcta gaccgcccga tgataggccg tttctcaacg
      541 ctagcccaat acctagtccc atcacagccc ccccaaacaa cgcacagatg attggatcgg
      601 tagataggac tgtttccggt aaaatatgga tacaaaggga ggtcaaagtg actgtgataa
      661 aagtgaaaat tgtaaacttc ttcccgattt ttaaccacgc caatacaaac aaaggtatat
      721 tcagtaaata caaagttgtt gataccggta cttcgaatcc tatcagtgat tttgacaaag
      781 tcgtcaaaat ctgagctaga ccagtaatac cacttgaata aatatgtcct ggctgataaa
      841 agaaattcaa tgcgactgat gcgatcaacg cataaacgat agataccgaa agcttggtcg
      901 tgtaatcatg ataagcccaa ttttcgaatg tttttttcat ggaaaagccc cgctttatat
      961 gttatggctt cattataaag tgtttttcaa ttaaatcaag gttgtcaaaa gaagcgtatt
     1021 cgacaaatcc tttttacctt tttatcggct attcttctat attcgttaca tcaatattca
     1081 attcgaacaa ttgtaaaggc gaaaccatac ttggtgcatc actcatcaca tctgctgctt
     1141 ttccgttttt aggaaaggca atgacttcac gaatattatc ttctccggct aataacattg
     1201 caaaacggtc caatcctaat gcaataccgc catgtggagg gaatccgtaa tctaaagctg
     1261 ttaacaagaa accaaattgt tcttccattt gttcttttgt aaagcctaga gtttctagta
     1321 ctttttcttg aagctcacga gtatgaatac ggatagaacc tccgcctagt tcataaccgt
     1381 tcaagacgat atcataagct tctgcataga cttttgcagg gtcttcagcc aataacggga
     1441 tatcttcttc ttttggcatc gtgaatggat ggtgagcaga gacatatcga ccttcttctt
     1501 ctgaaaattc aaattgtggc caatcagtta cccataagaa attgaatttc gattcatcaa
     1561 ttagtcctaa ttcttttcct aaacgtgtcc ggatagcacc taatgcagca gcgacgattt
     1621 cacttttatc tgcgccaaac atcagcaagt cacctggttt tgcatctgtt gctttaatga
     1681 ttgcttctgt tgcttctccc atgaatttag caattggacc tttcaatccg tcttcttcta
     1741 ctttcagcca tgcaagcccc ttagctccga attgtcctac atattgacct aactgatcca
     1801 tgtcttttcg tgagtagcga tctgcagcgc ctttagcatt caatgctttc acgacaccgc
     1861 cgttttcaag agccatttgg aagactttga attctacttc tttgactgta tcgcttagat
     1921 cgatcaattc catatcaaaa cgtgtatccg gtttgtcgct gccgtagcga gccattgctt
     1981 catcataggt catacgagga aatggcaatg tgacttcgat accgcgaact tctttcatta
     2041 ccttcgcaat caatccctct gtgtaagttt ggatttcttc tgctgttaag aacgttgttt
     2101 caatatctac ttgcgtaaat tcaggctgac ggtctccacg taaatcttca tcgcggaaac
     2161 aacgaacgat ttgataataa cggtcgaatc cagcattcat cagcaattgt ttaaacaatt
     2221 gaggagattg cggcaacgca tagaaatgtc cagcatggac acgtgaagga actaggtagt
     2281 ctcttgcgcc ttctggtgta gatttcccta ggtaaggtgt ttcgatatcg agaaagtcat
     2341 tatcatctaa ataatgacga atcgcttttg ttgtcgcatt tcttaatttg atgtttttag
     2401 tcatttttgg acgacgcaga tccaaataac gatatttcaa acgtatttcg tcactgattg
     2461 cttgatcatc ttcaatttga aatggcgggg tttttgcttg attcaaaatc acgatttcac
     2521 ttgcaatcac ttcgaattca ccagtggcca ttttaggatt tactgcattt tctccgcgtc
     2581 ttctaacttc cccagtcact tcgatcacgt attcactgcg acatttatcc gcgatttccc
     2641 atgcttcttt gttgatttca ggattaaaga cgatttgaac gattccttca cgatcacgta
     2701 ggtcgataaa gattactccc ccaaggtctc gtcttttttg tacccatcct tttaatgtaa
     2761 ctgtttgttc taattgttgt tcggaaacta atccgcaata gatcgttctt tgtgccattt
     2821 atttttttcc tcctactcga ataaagtcat ttcatcatag acttcatcaa aatgttcata
     2881 tatctcttct aatggaaaac ttttttcttt tcggcttgcc attgctttga cattaatcgt
     2941 ttgactagca agctcatctt caccgatcac caatactaat ttggcattcg ctttatctgc
     3001 tgtcttaaat tgcgcttttg ctttacggtt catgaagtcg cggtcagctg aaaaaccaaa
     3061 gtttcgaatc gcttggacca ctttcaatgc ttcgatattt gtttcgtctc cgagtgcgac
     3121 cacgtaagca tccaactcat taaatgcagg gataactact tcctctgcct ccatagtcaa
     3181 gagtaatcgc tcgataccca ttgcaaatcc gaagccaggt gttgaaggac cacctagttc
     3241 ttccactaga ttgtcataac gtccgcctgc gcaaatcgtt gcttgtgccc ccatttttgg
     3301 tgcgtcactc atgatttcaa aaatcgtatg tgtgtaataa tctagtccgc gaaccatatt
     3361 actgtcgatt tcataaggaa cagccaatgc atccagcatg gaaacaacgg tatcaaaatg
     3421 tttttgagca ggctcgctta gataatctaa gatagatgga gcttctgcca caaatttttg
     3481 atctcgtttg tctttactgt ccaatacgcg taatggattt tcatgaaggc ggcgtttaga
     3541 gtcttcactc agctctgatt caaaaggtaa aagatagtcg atcaatgctt gacgataggc
     3601 ttgacgcgtt tctttatcac ctaatgaatt gatgactagc cggatttgcg tgatccccaa
     3661 ctgtttgaag aaatcaagtg ccatcaccat gctctccaca tcagttgcag ggttttctga
     3721 tccaaatgct tctactccga tttgatggaa ttgacgtaac cgtccttttt gagggcgttc
     3781 gtagcgaaac attggtccca tataaaaagt tttgaacggt ttcgcatact ctgggccgaa
     3841 caatttatgc tcaacaaaag aacgaacgat tggtgctgta ccttctggtc gaagcgtcac
     3901 atggcgttcc cctttgtcat aaaaatcata catctctttt gaaacgatat ccgttgtatc
     3961 ccctacactt cgggatatca cttcatagtg ttcaaaaatc ggtgtacgca tttcattata
     4021 ctgataatct ctgaataata aacgtgctgt ttcttcgaca aactgccatt tttctgattc
     4081 gcctggcaaa atatcatttg ttccttttgg tctttggaaa ctcatagttt tctctccctt
     4141 tcttagctat gtaaataaaa aaatcaccct tgttcatcat gaacaagggt gaatgaaatt
     4201 catacacggt accacctaat tttgaaagat ttctctttcc tcttacgaat aacgctcgta
     4261 cgcgggacgg cttttcactc gtccatctcc agagtgtctt taggctaatg aaactttaga
     4321 tcattttcag cctcgatgat cctctcttgt aaagtttcaa aaacttactc gctctttcat
     4381 tgacattcga tattacttat aacctactaa aaaccagtct caaagtcaag agatatctga
     4441 ctatttcccc tcacaaaaac gttttagttc agctttttgg tattttgttt tagactagac
     4501 aaaaaggagg aatcagctat ggcagaaata agaatcggac attcacaagt ctacgcagaa
     4561 cagcttggat taggagcaaa tgccgttgga ggacataatc tttttgacgg tttggaagat
     4621 gagacaggaa aacaagtagt tcgtacagct ttgaatagcg ggatcaattt gatcgatacc
     4681 gcttatgctt acggaaacgg ccgttccgaa gaactgattg gcgaggtact gaaggaaaaa
     4741 gaatatgatc gctcacgtgt cgtcatcgct acaaaagctg ctcatgtacc aaataaaggg
     4801 cgaacatttg ataattctcc tgagtttctc aaacaatcgg tggaagatgc actaaaacgt
     4861 ctgcaaactg attacatcga cattttttat atccactttc cggatgaaag cacaccaaaa
     4921 aatgaatcag ttgctacatt gcacgaatta aaagaagcag gaaaaatccg agcagtcggt
     4981 gtgtcaaact tcacgttaga acaattgaaa gaagcgaatg cagatggata tgtcgatgta
     5041 gtagaagata aatacagttt gatccatcgg caggctgaaa aagaattatt cccttatctt
     5101 gaaaaaaaca aaatcagttt tgttccctat tttcctcttg cttccggttt gctgacagga
     5161 aaatatgaac ttggtgagga aaaacaattt ggtgaaggag atcctcgaaa aagaaatcca
     5221 gatttccaag gagaacgttt cagagaaatt ttaaccgctg tcgatgtact tcgccccatt
     5281 gctaaaagat accaagccac tcctgcgcaa cttgttttag cttggtacat gaagaatcca
     5341 cgagtgtctg tagttatacc tggagctaag cgtccagagc aagtatctga caatgtccaa
     5401 gctttagatt tgcatttatc caatgaagat tatcaaacca ttgatgaagc atttcgtgga
     5461 ttttgatcaa ttgaccttat caatttatag taatatgaaa aaaggaagat cagcactgtt
     5521 tgatcttcct ttttttcgat aagtttatgc taatctaatt cttgtaaagt agcgctcggc
     5581 tattcggata agtcggaaac tgattgaatg gaatcgtcca atcttgttca ggtacagaga
     5641 actcaaactt atttactagc tgatcggaaa acacacgtag actatgaaga gtgatccatt
     5701 ctcctgcaca gcgatgcatt tgtcgaaaat ttcccccgcc ttgtgcaatc atctcatatt
     5761 cttctttata ggaaatatct tttgctttcc ctacataccg tttgatcata aaggaatctg
     5821 gcgcttcaac tgttcgttca tcatggtctg ttccatataa atctaaaata acccaactgc
     5881 cttctggtat acgataacca tctacttcta cttctttcag cgaaatcgca ggtagcattg
     5941 ggaagaaagg ataatagcgt ctcatttctt gaataaacgg atcttgtaac gtcgaaaagt
     6001 cattttttaa ttgctgatac aaatctggtc tactgaaaag tgcatgtccc atcaatgccg
     6061 cccacacagt tagagcaacc gttggacgga tgatgttcaa taattcaaca gctgccactt
     6121 ccagaggtaa aagctgacca tctaaatctg tggcattggc aaaagcatat aatgccacat
     6181 tttcttttcc agccacagga ttttgtcgag cttcttcaat caagccttgc gcccattttt
     6241 ctgatttttt gcgattttcg acacctttta tgtgatcaat cggagaagta aacgttccgc
     6301 tgatcataga gatttgatat tctgctaatt tttcgacctc atctttagaa atcgctgata
     6361 aattgatacc tgcccactcg caaatagatg taaagaaaac catcttactt aaatcaaata
     6421 gttcaaattg accatgctgt gcttcaagtg cttgtgtcag attcttatct aagatacgat
     6481 gataatcttc cattctttcc ggtgtcatta gatccataaa aatatttttc cgatggtgat
     6541 gagcagctcc atccaaagtc tgtactccac cttgaccaaa aagtgtcttc aatactaatt
     6601 ttggcatagc accttttcgt ttgaaattcc ttggatcata aaatactttt gctgctgatg
     6661 atccgtagat cgtgatagct tctttattga aaatctttgc tttgacgacg ggagcgtccg
     6721 cttcatggcg taactcctcc aacatgttgt atcctttttg atacaatttt tttaaatctg
     6781 tgattttgat atctactact ggtacttctt tcatcttcag caacctccta taaaaaataa
     6841 cttgtataat ttaagcttaa ctttttttcc gactgctgac aaaagaagcg ctttttgcag
     6901 acaaaaaagc cacttaagtt agagtttatc agtctaactt aagcgacgtg tttcatagtt
     6961 tttactagtg aaaaccagat tactttattt gtttttagaa tcaataatga tcgttacagg
     7021 tccatcattg acgagagaaa ccgacatatc tgctccgaat tgtccggtag cgactgtgat
     7081 gccgcgactt ctgattcctt cattgaatga ttcgtataaa ggaatagctg tttctggtcg
     7141 tgcggcttcc acaaagcttg gtcgatttcc ttttttcgta tccgcaaaca gtgtgaattg
     7201 agagatactt aaaatttgtc ctcccacatc ttctacacta atattcattt ttccttgttc
     7261 atcttcaaaa atacgcattt gtgcaatttt ttttaccaga tagtccacat ctttttgcgt
     7321 atcggtttca tgaatgccta ataaaacaag taggccgtag ccaatttttc caatttcttg
     7381 ctgatcgact gacacagcag ctttacttac ccgttgaaca actgctctca tatctgtcct
     7441 cctatccatt cgtccggcgt acactgtaga catcaggtac tgctttgatt ttatccacga
     7501 tactctttag gtgggataaa ttctggatac caaccgtcaa atggatcgtc gccattttat
     7561 ctttattcgt ccgtgcttca acgctgttga gatttttcgt tagcgcattg actgtttgaa
     7621 gtacatcatt caataagcct gaacgattat agccgtaaat ttccaaatct gcatcatact
     7681 cttttcgtgt attcgatgta tcttcccatt cgacttcaat caaacgttct gctacatttg
     7741 gtttgtcagg ctgaacattc ggacaatctc gacgatgaat ggatatccct ctgcctttag
     7801 tgatataacc aacgatatca tcaccaggaa tcggattaca gcagcgactg atacgaatta
     7861 gtaagttttc taccccttga atgacaacgc caccttcatg acgtactttc attttgtcag
     7921 gttctttttt cggctgattc atgatttctt ctgcttcttg cttttgctgc tcgatttttt
     7981 gttctttacg ttctttttca gtcagacgat tggccatcgt caaggggcta acttctccat
     8041 accctacagc tgcatagaga tcatcttctg tttgataatt aaaacgatcg agtgcttctt
     8101 gcagtttgtt cttcgtcaaa atatctttag gcgtaaatcc tagatctgta atacatttga
     8161 ccacggattc gtggcctttg atcacatttt cttctcgatc ttgggctttg aagaaacgtt
     8221 tgatcttatt tcttgctttg ctagtagcaa ctaatttcaa ccagtcgcga cttgggccaa
     8281 atgaatttgg agaagtcatg atctcaatga tatctccgtt tttcaatttg taatcaagct
     8341 gcaccatttt gccatttact tttgcaccag tggttttgtt accgatatct gtatggatac
     8401 tgtatgcaaa gtccaatggt ccggatcctt ttggtaactc agtaacgtct ccttttggtg
     8461 tgaagacgta gactttatca ctaaagatat ctccttttac gccttccata aattcagaag
     8521 catcatagct ttcgtcttga agttcgagta tctcatggaa ccagcttaat tgtttcgtca
     8581 taccatctgg ttctactttt tcattttttc cttctttata ggcccagtgt gcagcaaccc
     8641 cgaattcagc gatttcatgc atttcctgtg ttcggatttg aatttctacc ggatttcctg
     8701 ccggaccgat cacggtagta tgcaaagatt ggtacatatt agcttttggc atggcaatat
     8761 aatccttaaa tctgccgggc attggtttcc attttgtgtg gattgctcct aatacagcat
     8821 aacaatcctt gattgaatct acgatcactc gaatagctag caagtcataa atttcgttga
     8881 actgcttttt ctgatctttc attttacgat agattgaata aatgtgtttt ggccgtccat
     8941 agatttctgc aaaaatcccc agctcttctg tcgctattcg aatatcttca acagtaccac
     9001 ttacgtattt ttcacgttct tctctcttcg tctgcatcaa gtggacgata cggtaatact
     9061 gttttggatt tagataacga agtgctgtat cttctaattc ccatttgatc cggctgatcc
     9121 ctagacgatg tgcaagaggc gcatagattt ctaaagtttc ctgagcgatc cttctttgct
     9181 tatcttctcg caggtgcttc aacgtacgca tgttatgtag tctatcagct aatttgacca
     9241 tgataactcg taaatcttga gccattgcta gcagcatctt tcggtggttt tctgctagct
     9301 gctcttcgtg ggatttatat tttattttcc ctaatttggt tacgccgtcc actaacatag
     9361 caacgtcatc gccaaattct tctctcagat cttctaaagt gatttctgta tcttcaacaa
     9421 catcatgtaa gaagcctgta gccactgtat ggggatccat ttttaaatct gctaatatac
     9481 ctgcgacttg gataggatga ataatgtagg gttcgcctga ttttctaaac tgtccgtcat
     9541 gtgcagcagt cgcgtattcg catgctttct caacaaatgc tacatgttcg ggacccatgt
     9601 attgactaac gagctttatg acaccgggtc ccgttaaaat ttcttctttt gtcacaggaa
     9661 tcgatccccc tttctttttt cggtaaaacg ggaatcggga caaatatctg tttgataatt
     9721 gtcccaaaat ccgtgcagtt atacgttttt ttacggaaat gttcgctttt taaaagcttt
     9781 ttcaaacgat ttttcgtatc cattgtataa ataattgttt ctattataca aattgttgac
     9841 tattttttca attctttctg tcgatactct tttattaatg aagaacgtta gctgaaagaa
     9901 ggattcatta attcccattg atagctagca gcacttaata aataaagagg tgctgtttct
     9961 gtacgaagga ttcttggtcc taatccacaa agtatccctt tgtgttctac aaaataatcg
    10021 acttcatcgc gggataatcc accttctggt ccaaagacag ctaagattcg actatctggt
    10081 tttgtttcac taagaatttt ggctgcattt gatttttcgc cttgctttgc tgattcctca
    10141 taagcaacca agaccacgtc ataatcatct agccgctcaa ataaatcttg tttcgtttcc
    10201 aataactcta ctgatggcac gatctgccga tgggattgtt ccgctgcctc ttgagcaatc
    10261 ttttcaagcc gttgttgtcg tttcatacgt ttttttgcat cccatttaac gacagaggct
    10321 gatgccggga aaccaagaaa agcttgcgct cctaattcag tggatttctg aacgatccaa
    10381 tccagctttt cccctttagg atagccactc gctatggtaa tcgaacttgg cagttccttt
    10441 tgattttctt cttttttaat ttctttcaga taaacagcct cttcagtaat ctccgtgatt
    10501 tctgcaacaa tggctatttg atcattgaat gccaaaaaaa cttcatcctc gggttccatc
    10561 ctcataaccc gtacaatgtg gtgatagttt tccccttcta aaacaaaata gtcttttgat
    10621 tcatatgatt cttttaaaaa ataccgctgc atctattcca cctctggttt tttcaaaata
    10681 attgcatacc agtctttttg ctggaaaatt tgatccacga taaacccttg ttctaacatg
    10741 gcatccagaa ccatttgctt cttctcttca atgattcctg aaacgatcag cgtgcctgtt
    10801 tgcttcagta aacgccaagc atctgggatc atcaataaga tgatatctgc taaaatgttc
    10861 gcaacgatta catcactttc aatctcgatt cctgtcaaaa gatcgttagc tgatacatgg
    10921 acatctttgg ctatttcatt catatccata ttttctttcg ccgaacgaac ggctacttcg
    10981 tctaaatcaa aggcatagac atcttttgca ccaagatatt ttgctgcaat acttagcacg
    11041 cccgacccag ttcccacatc tagcaatgtt tcaccaccac gcaagactgt ttctagtgct
    11101 gtcaatgtca aggaagtcgt gggatgagtg cctgtgccaa atgccatacc aggatctagt
    11161 gtgataattt tttctagagg atcttctgtc tggtagtttt cccagcttgg cgcaaccgtc
    11221 agcattcgcg taatacgcac cggatgatag tatttcttcc aggctgttgc ccagttgctt
    11281 tcctctactt cactaactgt tactttattt tttccaatag ctagaccaaa ctcaggtaat
    11341 tgtgtgatac gatctttgat aaaaggcaag atttccggta agaaagtcgt ttccggaaaa
    11401 taagccatga ctagtgcgcc ttctttcagc gatgtaaaat catttttatc taaaatctca
    11461 ccatattgat ccgactgaaa atgttcaaca tctaaagaat cttcaatcgc gacaccactc
    11521 gcccctgcct ccatcaaaat attagcaact gcttcgactg cttcactttc agtttctact
    11581 tttacttcat tccatttcat atctgtcact ccttaataac ttgggtacgg taaataagta
    11641 tctgcttcat tttgctgttt tacttctact aattctgcta gttgctgatt atcccagtga
    11701 agctcgaata cagcttcgtc atcgtcttca tccaaaaaat caagcaaatc gctttgccct
    11761 tgagctaata cttcatttag atattccact aaagaatcag ctactgattt tgccaaccct
    11821 ttttttcctt cataagggat tactgctaga taattgcgtt catcaaaaac agatttctga
    11881 ggattaaaaa gaagtacgcc gtcttcaaat tcgataattg gttcttgcga aacgatccct
    11941 tctgcatcat caatctcaaa ttgtgcttta ttttctgcaa acaagcgaac aacgatctcg
    12001 atcgtatgat tttttgtatc ccaatctaaa gctacatcat agtctgtcac ttgtttttca
    12061 attatttcat ctaaatatgt taatagatcc ttctttttca ttttctttcc tccaacccca
    12121 atctttctcg atttggtttt ttctttgtaa agagatataa ggatttcccg acacaacaaa
    12181 acgcaacggc ttttctgtcc atacaccttt gtttgggata ccgattctag gcaaagcatt
    12241 gatctttttt ggtgtctttt ttttctcaaa aactaagcgt aacgaactat ctgctataga
    12301 ctgaccataa agttcttgag gaattgctaa tgcttccact aattttcccg gaccgttgct
    12361 gatatctgga ccagtttttc cgccacgatt tttactcatt tgatcaatca ttgcggcggg
    12421 ttcgattgca cgtatcatta ctccttgagg aatcccttct ggctgagtaa tgatgttcaa
    12481 aatccgatga gtatgcatcg tgtaaaggta gatcgtacct ggtttttcat acattgcccg
    12541 cacacgcggt gttcttctta aaccatagct atgggcggcc tcgtcttctg gacctaggta
    12601 tgcttccgcg tctacaatat atccagctaa tcttccttct ggtgtgtcat gctccaaata
    12661 catccccaat aaatcccgtg ccacttctgg cgtcgtttta ttccaaaaaa attcatctat
    12721 cttttgttta tccgtcattt ttctcaccta ctttatcata cacaatatta gttggataag
    12781 ctatgaaaac aaaacaacag aaggctgttc tttctaggca tcttgctaaa tatgatataa
    12841 ttaagggacg taaaaacaag aaggaggaaa atcctatgca acagccattg gcttatcgca
    12901 tgagacctcg aacgatcgat gaagtcgttg gtcagcagca tctagtcggg gaaggaaaaa
    12961 tcatacgccg catggtcgat gctcgcatgc tctcttccat gattctatat ggacctcctg
    13021 gtactggaaa aacgagtatc gcaagtgcga tcgccggctc tactaattat gcatttcgca
    13081 tgctaaacgc cgctacagat tcaaaaaagg atctacaagt tgtagcggaa gaagcaaaaa
    13141 tgagtggtac agtgatcctc ttattggatg aagtacaccg attagataaa acaaaacagg
    13201 attttctgct tcctcattta gaaagtggcc gaatcatctt gatcggagcg actactgaga
    13261 atccttatat taccatcaat ccagcgatcc gcagccgtac acagatcttt gaagtcaaac
    13321 cattaacaga acaagacatc cagttggcag tcgaacatgc tttaagggat aaagaacgcg
    13381 gccttggaca acaagcaatc cagttagacg aagatgctct tcttcacctt tcgcgtgcaa
    13441 cgaacggcga tttgcggagt gcattgaatg ggctggaact ggctacacta tcgactcctt
    13501 cagataaaga aggaaggatt catcttacac tgtcgatcat tgaagaatgt gtccaaagaa
    13561 aagctcttac ccatgacaaa aatggagatg ctcattatga cgttatttcg gcttttcaaa
    13621 aatcgattcg tggaagtgat gtggatgctg cacttcatta tctcgcccgt ttagttgagg
    13681 caggtgatct ggcaagtatt tgccggcgtc tgatggttat cggttatgaa gatataggtt
    13741 taggtaatcc tgctgctgcg gctcgtacag tgaacgctgt attagctgct gaaaaattgg
    13801 gattgcctga ggcacgtatc ccactagcag acgtcgtcgt cgatctttgc ttgtcgccaa
    13861 aatccaattc tgcctacatg gcattagatg cagcacttgc tgatattcga gagggcaaag
    13921 ctggagatgt tcctgatcat ttgagagata gccattacaa aggtgcgaaa tcactcaacc
    13981 gtggcgtggg ttatcagtat cctcatcatt ttgatcaggc ttgggtcaat cagcaatatt
    14041 taccagataa actgaagaat gctcagtatt atcagccaaa agatacaggg aaatatgaac
    14101 aggctctggg tcaacagtat taccgtataa aagagtggaa aaagcatcct ccaaaaaact
    14161 agacaagaaa acggttctgt ttctgcgagc tctctcattg ctctttttaa attcatatgc
    14221 tactatagag gtgggaaatc tgtgatgttc gaattgtcct ctttgtgtgt tgaccgaaca
    14281 tttttattga tatcttggga cccgtcttgt gtcactgctg gtaacatgcc ttatcatttg
    14341 gtagttcgaa gctacgacca tgggacccac ctgcttgaat tcgcgggatc aatactacag
    14401 gacaaacaac ggcattaacg gagattacca tcttttaaag aataaagcaa tttgctcttg
    14461 gcaaattgct ttttctattt gttgcttatt ttggaggatt tgttcatgat taggatttta
    14521 atgattatcg ccactttagt attattattc gttagctatt atttatttaa taaacaggat
    14581 atcttttttg tattgatcaa caaaaatgat aaaaatcaag gattcctaca attttacgga
    14641 gctgcatatg ctgttttagg cgtaatgggt attttaacag cttttttcaa tcaacgcttt
    14701 attgccttgg tctttttact gatcgtaatt attgtttctg ccaccttcag tatccgattt
    14761 gcaaaaaaaa ttgctgaacc aaagcagtaa ataattctct ttttccctaa agtgttctac
    14821 aataaaagag tacaatacga ttgacttgag gagtgaatgc ttatgttaca acaatacaaa
    14881 aaaatcatgg ttgccgtaga tggttctgat gaagctgaat tagcttttaa aaaggcggtc
    14941 aatgtagcta tccgaaataa cggagaactt ctattagcac atgtcattga cacacgttct
    15001 ttccaaaccg tttcatcctt tgatggaatg cttgcagaac aagctactga aatggcaaag
    15061 caaacattgg cagattatga aagcaatgcg aagaaagctg gcttgaataa tgtaacctct
    15121 gtcgtagaat atggctcacc aaaacaaatt attgccagag aaatcccaga agataatcaa
    15181 gtagatttga ttatgttagg agccacagga ttgaatgctg tagaacgttt atttattggt
    15241 tctgtttctg aatatgttat tagaaacgca gcttgcgatg tgcttgttgt ccgaactgat
    15301 ttagaaaacc aattgccagc taaagaccac accaaagctt aacacgtaga cgacctgcca
    15361 actatcggca ggtttttttg cacaaaaaac cgtgataaaa acaaaagacg cggcaagcac
    15421 tcatcggtct ttataatgtt taaaataaag ggaaaacagt caatgagttc aagtaaaata
    15481 aaaaaaagtg aacaccaggg cgattagtcg ccctggtgtg gtttaaaacg acctaagcat
    15541 gccaaagctt attggttcgt caatgtggaa gtttccgatg aatctgatac gaaaaaatat
    15601 gaagtatcga tggcggatac cgattttatc gccaaactaa aattttattt cgggttcggg
    15661 taccgcgaga aggcaagtgc tacattcggt aaatcattca agaattgatg aaaggctacc
    15721 gcttgccatt gcaaccacaa cgatactcct gaacgcgatg ttggtgagat ttccaaagag
    15781 aagagctgtt taatgcaacg gtattaagca aatggcaaaa ggaagtcatg tagacaaagt
    15841 tttttatcaa acaccatatg atttcgcctg aacgctggtt tagctcagaa tacaatgatg
    15901 tggtccacga gacattaccc tcagtcagat aaaaaaagct ctctgacaga attacacatc
    15961 gataaatatt aagtaaatgt cagcgctagt tgaactttct gacactttca ggtacactgg
    16021 aaaagacaac tacacgaagg agggaaaata ctatgatgcg catgactaaa ataacaagta
    16081 atgaaaatta ccaacgatac catatcgcat ttagccatgc aattggtttt acagttgctg
    16141 ttgacgggac aagtttgtcc aaaacagttg aattcacgta aaatcaatgg ccatctcaag
    16201 gtatttttct catgataatc tgagtttaaa taacacaggc catggataac catggccttt
    16261 ttgagtggtc tcaaggagag agatcatgat catacaagca atttctatta aaaaaacaat
    16321 cggcgggaat ccattatttg aggatttatc ctgtcaaatt gaagctggtg aaaaaattgc
    16381 cttagtcggt atcaacggaa ctggcaaatc aaccttttta caaatcttga ccggcagaga
    16441 aggtgtcgac agcggtgtta tcagtcgcaa aaaaggctta aagatcggga cggtcgaaca
    16501 agaacttaca gtttccgaag cgaccgtcaa ccactacttg ctccattctg ccgctgagat
    16561 acaggacctc aaacagcaaa tgagtcgcta cgaagcattg atgacagaac cagatacaaa
    16621 cttagagaag tgtctggccc gttacggtga gctgcagcat cgctttgaag aactaggcgg
    16681 ttacgtatta gaagaccgga ttgcagcgat cttgcaaggc ttagggattc cacaccgtca
    16741 ggaagcacgg ttaacagaac ttagcggagg cgaacgagtt aaagtcgctt tagccaaaat
    16801 tttagcagcc gatgccgatt tacttttact cgatgaaccg accaatcatt tagatcttaa
    16861 cagtattcgc tggttggaaa actatttaca gaatacaaaa aaatcggttc tggtcgtttc
    16921 tcatgatcgg caatttctcg atcaagtcac cacaaaaaca tgggaactgg aagaaggtgc
    16981 attgattgag tatcccggaa actacagccg ttttcgagtc ttgaaagaag ctcgcttggc
    17041 tgagctgaca aaaaattacg aactgcagca aaaagaagtc cagcggttga aagtcatgat
    17101 tcgtcgtttt cgtcagtggg cacatgaagg agacaatgag tccttcttca aaaaagccaa
    17161 agaactggaa cgccgcctgg cgaaactgac actcgtcaaa ccgccacctc ccccgaaaaa
    17221 tcggctgcaa agcttgtcta atggtggaaa atctggcaaa gaagttttca tcattcaaaa
    17281 cttgcaccag cagtacgctg atcaagtact attcaaagac agcagttttg cagtctatcg
    17341 tggagatcat ttagccatca tcggcgacaa tggtgcagga aaatcaacct tactcaaatt
    17401 attactcggt gaggaaaagc cttacggtgg caccatcaaa ttaggtagta gcttgcaaat
    17461 cggttatttg ccgcagcagc tgcagtttgc cgatcccgat agccgtttgc tggcttacgc
    17521 catcacattg actggaaacg aagaagtcgc tcgacggcaa ttagccaaaa gtggctttta
    17581 tcaaagcgat gtgggtaaac ggatcaaaga tttatccggt ggtgaaaaaa tccgtttggc
    17641 gttgctgaaa ctctttttag aaaaaatcaa cgtcctgatt ttggatgaac caaccaatca
    17701 cctcgacagc tatgctcgag aagaaattga agaaatgctg caagactatc aaggaacgct
    17761 cctagcagtc agccatgacc gctacttttt gcagcaacac ttccaagaag cactcgttat
    17821 tgatcaggag cagatcagcc gccagccgct cggccaactg attgactagg acgttttttc
    17881 gcaacaaaaa acttgatttt catacctgtg ctttcacgat tcaggcaaag tacaggtgga
    17941 aaatcaagtt tttattattc gatcaatgaa tgtttcggat cggttttcta gctaaacata
    18001 aagcgaagaa taaagtcgac agcccacgag gctgatctaa aaatgttttt tctttttaca
    18061 tatgtaatgt cccttataaa gatgaatgaa aataaaatat tgggattttc ctgaaaatca
    18121 ataaataaaa tgccagtctt ttcccttttg accacttttc caagcaaaat tccctaagaa
    18181 gatcctgtaa aaaagaaggg aaattgaagt taaattacca ttttgcggtc ttctccgcaa
    18241 aaaaaacaca tgcaagaatt ccctcttgca tgcgtttcat cttttatcct aacgaatgct
    18301 ttggcttagc cgcgcagacg ctccacatca cgggcaatca ttagttcctc atctgttggg
    18361 atcactaata ctcttacttt tgattctttt gtagagatat ctacttcttc accacgcaat
    18421 tcattcaacg ctggatcgat ttcacaacca aaccaagaca tacctttgat gatttcacta
    18481 cgtacatgtc cgtcattctc gccgatacca gcagtaaaga caattgcatc aacaccattc
    18541 atcgttgtca cataaccgcc gatgtatttt cggatgcggt ccacgaaaat gtcataagct
    18601 aaacgaatat gttctttttc atagttcttt tcaagatcac gcatatcact agacaatcct
    18661 gttaggccta gcagaccaga tttcttgttc aaaacattga tcatttcatc aatatcaatc
    18721 tctaattttt ccattaaata tgggagaaca gctggatcga tatcacctga acgtgtaccc
    18781 attgtcaccc cagctaatgg tgtgaatccc atagaagtat ccatggattt acctccatcg
    18841 actgctgtga tcgatacacc atttcctaag tggcaagtga tgatttttag ttcttcgatc
    18901 ggtttgttca ataattttgc cgcacgttcc gacacataac gatgacttgt gccatggaag
    18961 ccatattttc ttactttgaa atcttcataa taagacaccg gtaagctata taaataattt
    19021 tgctttggca ttgttgagtg gaaagatgta tcgaatacag ccacactcaa aatgtcaggt
    19081 aaaatctttt taaacgcttt gatccccatt aagtttgcag gattatgcaa aggagcaaat
    19141 tctgctaatt cagcaatgcg gtccattact ttctcatcga tcactactga atccccataa
    19201 gtttctcctc cgtgaacaac acggtgccct actccggtga tttcatcata agagcttaaa
    19261 atttgcagtt caatcaactg atctaacagc atttttactg ctacttcgtg gttttcgata
    19321 tccatgattt gttcgaattt ttgattatcg ccgtacttga tggtaaaaat agaatcttgt
    19381 aaaccgatac gttcgacgat ccctttcgca actacggttt cttctggcat ttgatataat
    19441 tgccatttca aacttgaact cccagcattg attgcaattg ttttagacat atgactctcc
    19501 ttctcgttga atgattacag agcagatgat ttccagtttt taaactcgtt gaaaaattcc
    19561 gtcaccttct ctggttcttt taatgaagca agtttcacaa gcaagacttc ttttacttgt
    19621 tgtgcttgtg gtcctttttt ctgtaagatc aaaatacttt tttgcgattg tttattacgg
    19681 aacaattcat ctggcaattg aatcattcct tgaagataac cttcttcttt aaaccatttt
    19741 ttgatttctt cactttgatc cgtttctaaa aatccactcg gcatcaaaaa caaaccaaaa
    19801 ccatccggct tcacatattt cattgattgc tctagcaata aatgatgtgc ataactatgg
    19861 ccttcttcgg tacttgttaa aaactctttt gctttttcgt cattcggata ataaccgatt
    19921 ggcaaatcgc tgattgcaaa atcgacagga tctattaata attcctgcaa cccgtcttga
    19981 tgaaagtatt gaacatttgc ttgtgtcaaa tcacttgtag aagctgcgac agccaacaat
    20041 gtatcatcaa tatccacacc aattccttct gtttggtacc ccgcattcga taggtttaaa
    20101 agaactgtta ataacaagtt gcccattcct gccgcaatat ccagaatctt aaccggtgct
    20161 tttttatttg tgtatagttg ttcaataaga aaaacaaata aaaaaccgat actatcagga
    20221 gtgagttgat ggttcgcctg caaatgttct gtctggctgc ccttcaataa aagtaattga
    20281 gacagtctgc gccattcttc tggctcgaaa gaaagtttct tcaactcttc ataaagagct
    20341 gtgatccgtt gcgttgtctc ttttgtcggc actccatcca ccacacgtac ttgataatca
    20401 tctattaaat tttctgcatt ttcgacatag gcttctaaaa aggaagttcc caatgcattc
    20461 tgtaaaagct gaatagcttc caggttttga ttaaaccctt gttccatttt ttctggtaac
    20521 atggtgcacc tcatttctag atatattata acaatttttt cacctgttta attctatcga
    20581 acaattgaca aaatttcaat cgttttcact gttttttatt tgtgaaacgg caaacatttt
    20641 tttattaaaa acagtgttat aacagaaaag agggcgtttt cacgaacatt ccatcattct
    20701 gtactagtat ctgtatcatt ttttacttct tctgtgaacc gaaagataaa cggtgagact
    20761 tccacctcta agaataggga ctgttgtacc cactgatatg ttacagatcc agttgtgtag
    20821 taaaaaactc cttccgaact ttcggcaagc gtagactgtt ccgataaagc catctgcttc
    20881 attatttttg ctatataata atttttgatt cgtatattaa gatccattga caaacggtag
    20941 ctttctaatg ctgccatcaa taaaaacgta cataaaagaa ataaaagcaa gacagaaaat
    21001 aaaatagagc ccttataaca ttttctactg atcgtttttt gtccatatgg catatccatc
    21061 ttctccattt tcaaagtgta cataaaaaga aattgtgtgt tctttttcca ataattgaag
    21121 ctcagtgata cttgttaaca taggttgatg tccgccggaa gttcttcttt tacgtatcat
    21181 tgcctgatat ctttcgatga tatactgttt atcctcgatc gtgtagtgca gttgctgatt
    21241 tttcacagag acattgtgac tgctcttgag ttcttcttct aattgtgcta aaaatattag
    21301 ccactctttt tgatctttac gctgcaaaaa ttcacttact tggatggttt gctgaatcaa
    21361 aagattgaat gtgagcaata gactgctcaa tatcatcagt ccaattagac attcaatcag
    21421 cgtaaatgct ggaattactt gttttttcaa tactgatcgc ttctttctcc atattgacaa
    21481 acactttttc gattactcca ttattatcga caatttggat atcgtatggg atcatttctt
    21541 cccttcgctc tggtcttttg tgatacatcc gctgcgtctt gatatcttca taaatcattc
    21601 gaataaatag cagttcctcc ccacttttct tttctgcctg aagcaaaaac agctggccaa
    21661 agctcactaa gtagacacag acagaagcga tcataagcgc tgtaagggac tccaacaaaa
    21721 caaatcctgt tttattcaat tctttttgta tagcgaccac tccctatctg gaattgatac
    21781 gtaatcgtcc gctctttttc ttcccaataa aattgataag cttttagtga actttcattc
    21841 ccggttctag cagcaaaagt aatagaagca tggcgtttca aggtaatctc atcaggaata
    21901 tctaatactt cccaatttat tttatctgga ttcggaacat gaaaaatgat ttgattctct
    21961 tcactattcc aataaaagcc tgtttggatc tgattcacaa tagcgatttt ttgcgtagaa
    22021 tagattcgtt tttcaaattg gccgaaaaat tgatagactg ccaattcatt cttccatgca
    22081 gaaaatgcga gaactggaaa agaaaggatc gttgtaagta ataaaatcag taagagtgat
    22141 tcaaacaacg tatatcctga tgctaaaacc ttaccttcaa ggccgctgat agatttctgc
    22201 atgttctcgt ttgatatagc cttcattcaa caattcctcc acagtaggaa ctcttccatt
    22261 attttgaagc gcatagagct ctttttgtgt gtctaccaac tggatgatcg cttcacgact
    22321 ttcttggtct acatgattcc tataacgcga caaattaggg acgaacaaca acactaatac
    22381 actgatgatc agtaagacga ttagcatttc gatcaatgta aaaccgctat aataagatat
    22441 ttttctcttt ttcatggcaa aacctcctct atgcttccgt aaacaggtaa taaaacagcg
    22501 gcataaacta ataaaatcaa agccgctacg cccaaaaaaa tgattggctg aataaaatgc
    22561 atccagtgat gaagacgttt gaaaagctgt tttcttgtcc attcgctgta aaaaatcagt
    22621 tctttaccta aacttccctt tgcctctccc tgtaaaacaa ttttactgaa ttcttctgat
    22681 agaaaacgat aattctggaa ctgctcagcc aatgacatac ctgcctccaa tccttgaatc
    22741 aacttagagg caagatgctg aatcagactt tcttgtttcg tttcctttaa gcagactatg
    22801 atttgcctta attcgaaacc ttcatgaaaa agctttccaa tctctagtga caaataagcc
    22861 gattgataca aggaatatat cggtccaatc aataattgtc tgcttatcca ttcactctga
    22921 tcaacaggag acaacttcat cagacgatac cttactagta aagccaatag agtaaccgct
    22981 aggatgaata gtagaacata ccaatgaaag acttgtatca gctgaatacc ccaatgctct
    23041 tgcatgacca ttcctgaacc aataagctga ggcaaaacga acaaccgcat actaaacaag
    23101 atacctagca aaaaggtgag caatagcatc ggatatgcaa gcagcttttt caaatcattg
    23161 cgaaatcctt caacaagccg aaactgtttg gcaatttcca acaaagttgt cggcagattc
    23221 ccatgacgtt cagctaactc gatctgtacc agttgctctg ctccatatcc tatttgcgaa
    23281 agtacctcac taaacgcttg cccttccttt agctgatgtt ggatatttgc caaaataaca
    23341 ggtggaaacg aacggatcgt gagtaataaa taaagggatt cttgcagact aaaaccagat
    23401 aaaagcagtt ctcccatggt ttgtgcaaac tggttttgct ggcttcttgt taatccttta
    23461 gaatagttct gactgccaaa tattttcctg agcttcttca taactttcct cccatgattt
    23521 ttttactgat tcttcctcca taaattgata actccataaa acagaaggat ggtgattggc
    23581 atcttccaaa agttcttgat aaatgacacc agctaaacat tcagatagct cttctttttt
    23641 ctttgtcagt tcatggattc tgaccaatgt accttcaaga ttgcgtgcat gtacggttgc
    23701 aaaaacccgg tgccctgtta gtgctgctct taccgcagca tgtgcagttg attcatctct
    23761 tatctcgcca atgattagca agtctggacg atgccgtaat gcgagtctca aaagtgtatc
    23821 ataagtctgt gctattttcg gattgatctg tagctgcaga aaaaacggtt cttctatctc
    23881 tacaggatct tcgatagtaa tcacttgcaa tttttcttcc aaagccaacc gatacattag
    23941 agaagtcttg cctgatccga ctggtccgct gaaaaggtat aatccacgtc tgacggtttt
    24001 ctgtaaaata gttgcaaaat ctcgagaact aaagcaacga tggggtgttt caaaagtacc
    24061 gtataatagc cgaatcacta aactctctcg ttgaaggtaa tctcctacag aagacaaccg
    24121 cagacgaact tcctgttttt ctaaagaata agtaaatgca cctaactgag cttttctttt
    24181 ttctccgata tccatctgtc ccaaatactt aaatcttgag atcagttgct gtccatctgc
    24241 cgaagagact ttcttccaga atgttcgttt ttttccatag cgaaaataga tgtaaaatac
    24301 ttctgaagct ggcaatatat atacgtcttg ggcatgtttg gcaaaacttt cacttatcat
    24361 ttgatccgat aatcgtttta tttccattat tctcccctcc acaaaaaaca tacgtctttt
    24421 tgatcttttt gtttcaaata aaatgaattt acaaaaaaat attggcaaaa accgaattta
    24481 tgttatactt ataaaaattt atcttgggag gcactccatt ggaaacaaca ttaaggaaat
    24541 atatcgaagt agcaagtaaa aaaaagcctg ctgacctagt gatcaagcat gctaagatcg
    24601 tcaacgtctt tacgaaagaa atcatggaaa aagatgtagc aatctgcgaa ggaatgatcg
    24661 taggaatcgg tgaatacgaa ggaaaaagcg tacataatgc taatgggcaa tatctagtgc
    24721 caggattcat cgatggccat gtgcatatcg aaagctcact tttatcacca agcgaatttt
    24781 caaaagtatc cttgcttcat ggagttacta ctgtagtgac agatccccat gaaataggaa
    24841 acattgctgg gagtacaggt cttgagttta tgatagaaga tgcaagacaa acactcatga
    24901 atatcttttt tatgctacct tcttgtgtac ctgtaacccc ttttgagacc aatggtgcaa
    24961 atttaaatgc aaaaaaatta gccccttttt tacaaaaacc cgaagtttta ggcttagctg
    25021 aagtcatgaa ctatcaagca gtagcaaata atgaaacaga catcctagaa aagatccaat
    25081 tgatgcacca gcataagaaa aaaattgacg ggcatgcagc tggtatcggc atggaagagt
    25141 taaatgttta tcctgcagca ggtattcgga cggatcatga agctacaact gcaaaagaag
    25201 ctaaagaacg tttggatctt ggtatgtatt taatggttcg agaagggacc gttgcaaaag
    25261 atctggcatc gctactaccg gcgatcacac ctgaaaattc aagaagatgc ttctttgtca
    25321 ccgatgataa actgatcaat gatttagtaa aagaaggatc aatcgatcat atcgtccgtc
    25381 aagctattgc acttggaatc gatccattac aagccattca gatggcaaca ttgaatgcag
    25441 cagaatgttt tgatttgaaa acactaggtg cgattgcccc tgggtatcaa gctgatttct
    25501 tcttaacaga cgatcttacc aagctgccaa ttcatgaagt tttctataag ggaacattag
    25561 ttgttcaatc tgggcaactc caagaccatt tatttgctga tcattcaaat tcatatacgt
    25621 gttgtctgcc aaaactaaac gctcaaccat taaatccaca ttcgttagag ctgcttcttt
    25681 cttcttcaca agcccatgtg atcgaaatta tcccgaatag tctagtgact aaagatttgg
    25741 tagaaaaagt ggaccgacaa ggcgaacgat tcattccttc aatcgaaaaa gaccaattaa
    25801 agattgcggt gatcgagcgt caccatcaaa cgggaaacat tggattaggc atagtgaaag
    25861 gatttcagct gaaacaagga gcgatagcga caacgattgc ccatgactcg cataatctag
    25921 ttgtcgttgg gacaaacgac gaggatatgc tgtatgctgc taatcttttg attcaaaaag
    25981 gtggcgggat gaccgttgtc aacaaacagc aggaaatagc ttgtgtttct cttcctatag
    26041 gcggaatcat gacacaggaa cctttttcga tcgtctatga acagttgaat gaattaataa
    26101 ctaaagcaca tcaactagga gcagctaaaa cctttgatcc ttttttgaca ctttctttct
    26161 taacattatc tgtcataccc gaattgaaga tcaccgacaa aggattattt tcattttctc
    26221 gcttccaatt gattccacct tgtggaaaaa ccgatgaaat aaatacattt taaatcaaat
    26281 aaatcccaga aaatacatta catctgtaat gttcattttc tgggatttat ttatggatat
    26341 taatgttttt atgaaagcag aaagttagtt tcacagactt ttctttttgg tcttatcttg
    26401 aaattgagct tctactcgat aaatacgttg accttttgaa gaaaattttt cttcatactc
    26461 cgtcataata ttgccttcaa attgactttg atgtaaatct aaccatacct gtttgaggat
    26521 catgccatat tgagaaaaac ttgccagaga atactcaaac aatccttgat tatctgtttt
    26581 gaaatggatt tctccatttg gacgaagaat ctgttcatct acagcaagaa aagtcttata
    26641 tgtcaatcgc cgtttttcat gacgtttttt tggccatgga tcggaaaaat tcagatagat
    26701 ttgatctact tcgctgtcag cgaaatactc tgtcaacgct gatccatcca catgtagaag
    26761 tttcaggtta ggaagcggtt gttcgatcaa cttgtctagt gcaatcgaaa caacactgac
    26821 ctgcatttct actccaatat aattgatttc aggatgggct tttgccattc cagtgatgaa
    26881 ctggcctttc cccataccaa tctcaatgtg gattggatga tcgttttcaa acaattcatt
    26941 ccattttccc ttccacaatg ttggatcact aattacaaaa ttcgggtgag ccgctaacat
    27001 ttccgctgct cccggacgat ttcggacacg cattatttat ctccattcct ttcctctaat
    27061 taaaaaggtc tggccaaatc tccgcagagt ttgggccaaa cctcagccac tgaaaattcg
    27121 ttttaattgt aaaatttctt cgttcatccg tcgatgatct tcacgtttgc aatatctggt
    27181 gatttcttgg agcaaattca tggccgcata ccaatgaaat ttttcaagca tttcgtcagt
    27241 cgggcgtatg ccataactga tcagccactg attccagcta gacagtggca cataatttcc
    27301 taaaatcgtt gcaatatcca ccgcagggtc tgcaaacatg attgaatccc aatcgactag
    27361 atataaataa tttttacata ctaaccaatt acgatggttt acatctccgt gtactactga
    27421 taagttttcc ttatctattg acgggatctg ctcacgaaga aaacgatgga caatttgcag
    27481 aaattgattt ttctggagtt cttccggtaa ttctttttca tatttttgca gcatcttctc
    27541 cggcgtcatg acacgaccac cgatacgggt cagcatggtt ttaagagaat ttgaatgatg
    27601 taaatggtac aaaacatcaa tcacgtcgtt tctttgacca atctcattag gagtcagcaa
    27661 gcgaccttcc agccactctt gtgctgtcaa agtatcgcca ttccccgttc gttttgtcca
    27721 aacaagtttt ggtgtaattc cttcccttga aagagcagcc aacatcggag tcgtgtttcg
    27781 tttaataaag actttgtcct catcacgcat gcctatgtat gtttttcctg tgtcgccttt
    27841 gatgggacgt aaccgccact cttggtctaa ctgaaactcc atcatgcatt ctcccgtcct
    27901 cttactcttt gttttgcagt acttattatt ctagtcgcct gaacactaca cgtcaagcac
    27961 atatacgttc tttttcattt aattgccatt caaaaaacca gcttttctca aaaaaaatag
    28021 aacgaacagc attcttactg tcaacgctct atttttcatt ctcattttta ccctacacag
    28081 ctttgttttc agtctgacat cttcttcaaa cgagttggta catatagttt aacaaagagc
    28141 atagtaacaa tcaaataagc cgctagcgga acccatcgct gctgcccatc taatacgact
    28201 gcagcaatca aaccaaaaat aaccgacgcg ataattaata cccatgtgag tagatactga
    28261 atcgcctgct tcttctgatt cttatggatt ggataaagct gaacgagaac catatagcgg
    28321 aactggttcg ctaaaggtaa cagttggaaa ccgatcaaat ataaaaataa gaaaccgata
    28381 atcagactaa aataaaagtc gactactccg gcaatcaata acccacctat aattactaat
    28441 cggaaataaa gaccactaaa atcagaacct cttgccaatc ttcttgcaaa caagtaaaga
    28501 taagtgtttt ttgattctct tttaatatga gtcaatatag gatccaaata gcgtcttctt
    28561 ttgatctttg ccgtaatttc aggaacatct gtgaataaat tgataaattg atagatctga
    28621 tgcaacctat gttcttctac atgcaccatt ttctcccaat ccaatccttt ttcttgttgc
    28681 ttccacaaaa accagtaata tagacttgcc tgtactaatg cacctgctat tcctgcccat
    28741 attagccaat aaaatgatag aaacaaaaag atcagagaag aaaaaaacca gcctaatttc
    28801 caataacgaa taacttgttg atctagtcga aacaattctg ctctcttgat ttgcagatga
    28861 ctgaatttca aactccataa tatgatcaga tagacgaaaa acatcgaaaa ggattgctcc
    28921 gtactaacca ccactaatgg catggccaat ccgcatgcca atatcaaaaa gatcaacgga
    28981 aaaatgcaag aatagcgaaa agctcgttcc aaataggttt gcatttcttt ttctttagga
    29041 agtaaaaaaa caatatctgc tgcctctgca aaagtagcca tcttacccac gaataaacag
    29101 agcatccaaa atacagagat tatgagccct ccaattgcaa acggtggctg cagggttttc
    29161 aaccaattag aataatataa tcccacaccg ccaatcaaga aaagacaaac taaaacaaaa
    29221 tgatcgttca atacataacg catatatttc atcattttct tttgatgtct tgctaaacgt
    29281 ttttgaaaaa aactactcat gtgcctgctc ctttgttaaa gcaatataga tttcgtccaa
    29341 agaagaatcc ggcagctgaa attcttttct caacatctca agagaaccgt ctgctcgtat
    29401 ctctccgtta tgtaagacca caaaacgatc acaatatttt tctgcagtag ctaaaatatg
    29461 cgtagacatc aaaataccgg ctccttcttt tttcatttct accatcaatt caagcaatgc
    29521 attgatagct agtggatcca agcctaaaaa tggttcgtcg atgatatata aagaaggctc
    29581 gatcaaaaag gcgcataaga tcatcacttt ctgtttcatt ccctttgaaa aattagctgg
    29641 gaaccagtca agacgattag ataaccgaaa agtctccaac aacttttctg ctcttttcat
    29701 tgctatatcc ataggtatat cgtaggccat cgcagtgact tcgatatgtt ctctcaatgt
    29761 caattcttca tataaagaag gagtctctgg aatataccca atttgtttgc gatattcttc
    29821 cgtatttttc tttaaggata gatgattgat ttctatcgtt ccttttgaag cttccagcag
    29881 accgataatg tgcttgatgg tcgtactttt ccctgctccg ttcagtccaa tcaaccctac
    29941 tatctctcca ggctgtacat cgaaactgat atcttttaac acagggagat gtccgtaccc
    30001 ccctgtgaca tgatcgataa ttaaactcat tctttttcct ccgccattta tttttattta
    30061 ttataccacg aagaaaatga cggtattttg tttttttcct tacaatagct ttaacttatg
    30121 gtaaagttaa gctgaagtat ttattcttta tagaaaggat gttttatatg gaaaactgta
    30181 ttttttgcaa aatcatcaat caagaaatcc caagctataa aatatatgaa gacgacaagg
    30241 tctatgcttt tttagacatc tctcaagcaa cgaaaggaca cacattagtt gtgcctaaac
    30301 gtcatgtagc agatattttt gaatatgatc ccgaacttgc aggagaggtt ttttcgcgtg
    30361 tgcctaagat tgctcaagca ttggaaaaag cttttccaga aatgaaaggt cttaatatct
    30421 tgaataacaa ccgtgaacta gcctatcaat ctgtttttca ttcacatatc catttagtcc
    30481 ctcgctacag caaagaggat gatttttcta ttcactttgt caatcatcaa gatagttatg
    30541 gatcggaaga actaaaagct attcaagaaa caatcgtaaa gcaggtgtcc tgcgatgatt
    30601 aaaaactttt tcaaaggact gttatttgga gctgctgctg gcactgtcgg aggtctgtta
    30661 ttggcgccca ggagcgggaa tgaaacaaga aagaaactga tcgaagaatt ggaagaagca
    30721 acaactctga caaatgattt aaataacagc ctgaatcatt ttaaaagcgc attgatcgaa
    30781 acaaaagaaa cagctgaatc tgtgattcct gctttccaag aagcaatcaa aaaagatatc
    30841 gaagatttta aattccaagc cgaaccacgt atcacacaaa tcaatgaaca agtagaaaaa
    30901 ctaaaagctg atctgccaga gtatcctgaa caaatataat aatgattatt ttctcaacag
    30961 atttaactat ataagtgaga aaagtttgcg tatttcctag gaatgtgatg aaattttgaa
    31021 ttttgtttga aactaccacg acaac
//
LOCUS       DACDHJ010000010        30544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDHJ010000010 DACDHJ010000000
VERSION     DACDHJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.010"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            137..1006
                     /locus_tag="IV223_000389"
     CDS             137..1006
                     /locus_tag="IV223_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8710699.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1215..2240
                     /locus_tag="IV223_000390"
     CDS             1215..2240
                     /locus_tag="IV223_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8710700.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2279..3229
                     /gene="manA"
                     /locus_tag="IV223_000391"
     CDS             2279..3229
                     /gene="manA"
                     /locus_tag="IV223_000391"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8710701.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3323..5026
                     /locus_tag="IV223_000392"
     CDS             3323..5026
                     /locus_tag="IV223_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8710702.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5028..7061
                     /locus_tag="IV223_000393"
     CDS             5028..7061
                     /locus_tag="IV223_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8710703.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7072..7512
                     /locus_tag="IV223_000394"
     CDS             7072..7512
                     /locus_tag="IV223_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8710704.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7712..8839)
                     /locus_tag="IV223_000395"
     CDS             complement(7712..8839)
                     /locus_tag="IV223_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710705.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8842..9519)
                     /locus_tag="IV223_000396"
     CDS             complement(8842..9519)
                     /locus_tag="IV223_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710706.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9783..11549
                     /locus_tag="IV223_000397"
     CDS             9783..11549
                     /locus_tag="IV223_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8710707.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11564..12001
                     /locus_tag="IV223_000398"
     CDS             11564..12001
                     /locus_tag="IV223_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8710708.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12014..13165
                     /locus_tag="IV223_000399"
     CDS             12014..13165
                     /locus_tag="IV223_000399"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8710709.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13227..13925)
                     /locus_tag="IV223_000400"
     CDS             complement(13227..13925)
                     /locus_tag="IV223_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8710710.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13918..15270)
                     /locus_tag="IV223_000401"
     CDS             complement(13918..15270)
                     /locus_tag="IV223_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8710711.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15493..16893)
                     /locus_tag="IV223_000402"
     CDS             complement(15493..16893)
                     /locus_tag="IV223_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8710712.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17054..17791
                     /locus_tag="IV223_000403"
     CDS             17054..17791
                     /locus_tag="IV223_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8710713.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17916..18395
                     /locus_tag="IV223_000404"
     CDS             17916..18395
                     /locus_tag="IV223_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8710714.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18392..19159
                     /locus_tag="IV223_000405"
     CDS             18392..19159
                     /locus_tag="IV223_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710715.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19389..19958
                     /gene="lepB"
                     /locus_tag="IV223_000406"
     CDS             19389..19958
                     /gene="lepB"
                     /locus_tag="IV223_000406"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8710716.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20062..20664)
                     /locus_tag="IV223_000407"
     CDS             complement(20062..20664)
                     /locus_tag="IV223_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8710717.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20770..21239
                     /locus_tag="IV223_000408"
                     /pseudo
     CDS             20770..21239
                     /locus_tag="IV223_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21345..21929
                     /locus_tag="IV223_000409"
     CDS             21345..21929
                     /locus_tag="IV223_000409"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8710718.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21932..23005
                     /gene="prfA"
                     /locus_tag="IV223_000410"
     CDS             21932..23005
                     /gene="prfA"
                     /locus_tag="IV223_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8710719.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            22998..23837
                     /gene="prmC"
                     /locus_tag="IV223_000411"
     CDS             22998..23837
                     /gene="prmC"
                     /locus_tag="IV223_000411"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8710720.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23830..24009
                     /locus_tag="IV223_000412"
     CDS             23830..24009
                     /locus_tag="IV223_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710721.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24031..25056
                     /locus_tag="IV223_000413"
     CDS             24031..25056
                     /locus_tag="IV223_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8710722.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25231..26475
                     /locus_tag="IV223_000414"
     CDS             25231..26475
                     /locus_tag="IV223_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8710723.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26628..27575
                     /locus_tag="IV223_000415"
     CDS             26628..27575
                     /locus_tag="IV223_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8710724.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27709..28338
                     /gene="upp"
                     /locus_tag="IV223_000416"
     CDS             27709..28338
                     /gene="upp"
                     /locus_tag="IV223_000416"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8710725.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28477..28719)
                     /locus_tag="IV223_000417"
     CDS             complement(28477..28719)
                     /locus_tag="IV223_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710726.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28840..28912
                     /locus_tag="IV223_000418"
     tRNA            28840..28912
                     /locus_tag="IV223_000418"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28873..28875,aa:Thr,seq:cgt)
     gene            complement(29031..29669)
                     /locus_tag="IV223_000419"
     CDS             complement(29031..29669)
                     /locus_tag="IV223_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8710727.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29792..30424)
                     /locus_tag="IV223_000420"
     CDS             complement(29792..30424)
                     /locus_tag="IV223_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8710728.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10394 a         5372 c         6167 g         8611 t
ORIGIN      
        1 tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca ttttttttaa
       61 caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa gcttgacgta
      121 gaggggagca agaaatatga aagattttca attgggggct gccacctatt taaaaagtgt
      181 agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg ttgttggctt
      241 tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag ctggaagtga
      301 aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga tcaaaaagct
      361 gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc tttgtcgtat
      421 ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta tggggatttt
      481 gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata agaacgacca
      541 aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg aagaaaaatt
      601 cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta ctaacttaac
      661 aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg aagaagaggg
      721 catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta gcggaggcgc
      781 aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca tgatcgatac
      841 tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt tcttcttaga
      901 caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt ggttcgtcag
      961 tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt ttctttctta
     1021 acgattgtcg catgtttcgt cattttatga acggtcatac tatcgcttat tctagagata
     1081 gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt tttttgtgta
     1141 aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat ctgatagaaa
     1201 agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag atggaagaag
     1261 gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa acatttacag
     1321 ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg ttgtttgatg
     1381 cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta ttagaagatg
     1441 gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa aatcagacaa
     1501 tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata ccaaacagag
     1561 ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca ggtgagcaac
     1621 taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg atgctagagg
     1681 aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa ccattaggaa
     1741 tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca gaacaactgc
     1801 cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt ttggctgaac
     1861 atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt gtccaaatgt
     1921 atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg attgattctt
     1981 tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg gacttaaaaa
     2041 aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt acgtttttct
     2101 tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta catcctgtcg
     2161 atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt ttgaaagaga
     2221 aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag tttgaattat
     2281 gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag gaagtcgttt
     2341 acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt gggcgatcag
     2401 tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga aaagattaga
     2461 tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag tgtttccatt
     2521 attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc cagatgatgc
     2581 ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata tcatcgatgc
     2641 tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag aactagctga
     2701 gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta aaaaaggtga
     2761 tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca tgattttaga
     2821 aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa aagatgcgca
     2881 aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg tccctgcgaa
     2941 aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg tcacttattt
     3001 agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat ctttcaaaaa
     3061 acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg tcgttgacgg
     3121 aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg tttctgagtg
     3181 ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt tcccagatag
     3241 ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc gtccatcaat
     3301 aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca gcagtcgcgt
     3361 gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca tgccaaatct
     3421 aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt ctggcgattt
     3481 gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac ccatcttgat
     3541 cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg gtgcaattgc
     3601 ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga tgattatcgg
     3661 gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga aattcaaggc
     3721 tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg ttatttgtat
     3781 aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc tcgcctccgt
     3841 tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct tagaacctct
     3901 aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt taggaatcga
     3961 attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc ctggacctgg
     4021 tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag taaatgcgag
     4081 tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc catttgtctt
     4141 gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca cgttgatatt
     4201 tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac tcgtgatttt
     4261 tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa tcagtacatt
     4321 tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa aagagaacaa
     4381 aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg tcatttttgc
     4441 ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa agaaattagc
     4501 agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga ataaccaagt
     4561 aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa aagtacctca
     4621 aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt cacgaataaa
     4681 agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata tcacagaaca
     4741 aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg gctcacaaac
     4801 tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt ttgttatctc
     4861 aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg cacaaagaga
     4921 actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag ttgaacattg
     4981 ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg tatttatcgc
     5041 ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta tctatccaaa
     5101 aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta gataatcttg
     5161 aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc tcatttgtca
     5221 ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg aaaagtaatg
     5281 acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg acgacagagc
     5341 cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact ttttatgcag
     5401 atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt cggaaccagg
     5461 gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat gtattagaat
     5521 tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca ctgaattatt
     5581 tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc ggggaagaat
     5641 tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt ttgatgctga
     5701 cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca tataatagtc
     5761 tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc gcttctgaga
     5821 caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta aatgacttca
     5881 cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt gcttatttag
     5941 taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat gaagatgaaa
     6001 ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc gttttacaag
     6061 aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag gaagttgcga
     6121 atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc gaagaagaga
     6181 tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa gaaagcaata
     6241 tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg gaactacgat
     6301 tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct gatttaaaaa
     6361 aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta ccaggatacg
     6421 gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag cagctaaaag
     6481 aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac agcaataacg
     6541 agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa atcaatcaat
     6601 tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc gaaacgattc
     6661 gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg gttcatcgcc
     6721 ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat atggggttat
     6781 tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat ttgaaacatc
     6841 cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg ttagttatgc
     6901 tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc agtggagcaa
     6961 tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa atcgtttatc
     7021 agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa gatgatacag
     7081 ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga agcgttgacc
     7141 gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat ccaatcaatg
     7201 atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt gccacatggg
     7261 actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt gcctgatggt
     7321 gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt agtggcttta
     7381 aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc agacatcgac
     7441 caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat ttttgaacat
     7501 ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt aataacttca
     7561 accaagataa aaataagagg agctattttt gttgggaggt tcttattact gcggatgcaa
     7621 gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac ggtgacaagg
     7681 tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata tagatagtgc
     7741 ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata ccattcatga
     7801 aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc acattttgaa
     7861 aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca atcgaagaaa
     7921 acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg actagagaat
     7981 gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc cacaacaaca
     8041 ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc tcctgaccaa
     8101 tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt actagtatag
     8161 agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa atggtaaaac
     8221 ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag attacctgct
     8281 ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat aaaatcaaaa
     8341 aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag agatgcgctg
     8401 gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg agcaactcga
     8461 cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc agtcgatcca
     8521 gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc cctgtgtttt
     8581 ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc acaaattctt
     8641 tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca tagttatgat
     8701 tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg aatgcaaaga
     8761 gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag agattgcgtt
     8821 caaatgtaag taaagacata gttacacctc atataattta cgataaatag ctaaaatctc
     8881 ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg ctttagaaca
     8941 aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc cctctttctg
     9001 ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc cgttcaacgg
     9061 ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca agacatgttg
     9121 tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca tcttcaatcg
     9181 ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca aagtcaaata
     9241 atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa agaaagaccg
     9301 gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat cataagttgt
     9361 tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt tcggggcaat
     9421 caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca cacgtttttt
     9481 accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc caatataaaa
     9541 tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag ttattgtttg
     9601 catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc tgataccgaa
     9661 atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc caaagtacga
     9721 ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga gggagagaca
     9781 aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta caaaaattag
     9841 gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc tggggtgtat
     9901 taacggctct atttatccca gatggttatt taccaaatga ggctttcgct accatggtaa
     9961 gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga aaagtaattg
    10021 ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt gtcggaacag
    10081 atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat gccatcaaga
    10141 aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta gtcaataatt
    10201 tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg attggtccag
    10261 ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta tctgcccatt
    10321 tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg aataatgcaa
    10381 tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct ggaaaatcta
    10441 ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt gcttttactt
    10501 tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt cactttttag
    10561 gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta ttcttatcag
    10621 ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct ggactaagag
    10681 cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc aattcttacc
    10741 ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt tctgcagtaa
    10801 ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt caagagacaa
    10861 aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct acattctctg
    10921 gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg atgggcgctt
    10981 cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc aatcaagcaa
    11041 tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa ctacaagaaa
    11101 gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt ttgaattcac
    11161 ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca gtttctgaaa
    11221 ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc aaagagattc
    11281 tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg ctcaaagata
    11341 tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa gatttaaaag
    11401 atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta cagatggctg
    11461 cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa tacgaatcga
    11521 ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa gtttgccatt
    11581 cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc gctattgcgg
    11641 tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca tggtagatag
    11701 agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg gaacagatga
    11761 agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag atggtgtaga
    11821 ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg caggagtcgg
    11881 agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata tggacaatgt
    11941 tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag caattgctta
    12001 aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc gaggatttat
    12061 tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga atcacacgat
    12121 cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg aatcaaaaga
    12181 aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag aaaaagtaat
    12241 ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata tcttaccttt
    12301 tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa gcacaacatc
    12361 tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag aaaaagaagt
    12421 gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc caaatgcagc
    12481 agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc aagtggaacc
    12541 tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc aattaaaagg
    12601 ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta gtgtcaatac
    12661 tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca ttgatgaagc
    12721 aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa caggaagttt
    12781 gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg aaaaaatcat
    12841 tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc ggacaccatt
    12901 acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac aagaacataa
    12961 cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact atgatcctga
    13021 agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg aacaattgat
    13081 tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc aaaacgtgaa
    13141 ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt gacgaaattt
    13201 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
    13261 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
    13321 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
    13381 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
    13441 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
    13501 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
    13561 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
    13621 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
    13681 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
    13741 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
    13801 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
    13861 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
    13921 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
    13981 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
    14041 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
    14101 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
    14161 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
    14221 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
    14281 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
    14341 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
    14401 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
    14461 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
    14521 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
    14581 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
    14641 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
    14701 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
    14761 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
    14821 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
    14881 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
    14941 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
    15001 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
    15061 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
    15121 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
    15181 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
    15241 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
    15301 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
    15361 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
    15421 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
    15481 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
    15541 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
    15601 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
    15661 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
    15721 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
    15781 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
    15841 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
    15901 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
    15961 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
    16021 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
    16081 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
    16141 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
    16201 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
    16261 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
    16321 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
    16381 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
    16441 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
    16501 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
    16561 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
    16621 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
    16681 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
    16741 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
    16801 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
    16861 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
    16921 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
    16981 cagctttcga ttaaaataga aatggcttat aataaggcaa atcctagaaa aaagaaggag
    17041 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
    17101 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
    17161 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
    17221 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
    17281 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
    17341 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
    17401 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
    17461 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
    17521 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
    17581 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
    17641 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
    17701 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
    17761 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
    17821 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
    17881 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
    17941 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
    18001 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
    18061 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
    18121 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
    18181 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
    18241 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
    18301 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
    18361 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
    18421 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
    18481 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
    18541 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
    18601 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
    18661 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
    18721 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
    18781 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
    18841 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
    18901 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
    18961 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
    19021 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
    19081 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
    19141 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
    19201 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
    19261 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
    19321 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
    19381 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
    19441 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
    19501 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
    19561 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
    19621 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
    19681 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
    19741 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
    19801 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
    19861 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
    19921 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
    19981 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
    20041 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
    20101 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
    20161 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
    20221 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
    20281 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
    20341 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
    20401 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
    20461 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
    20521 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
    20581 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
    20641 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
    20701 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
    20761 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
    20821 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
    20881 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
    20941 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
    21001 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
    21061 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
    21121 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
    21181 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
    21241 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
    21301 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
    21361 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
    21421 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
    21481 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
    21541 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
    21601 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
    21661 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
    21721 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
    21781 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
    21841 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
    21901 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
    21961 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
    22021 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
    22081 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
    22141 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
    22201 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
    22261 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
    22321 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
    22381 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
    22441 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
    22501 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
    22561 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
    22621 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
    22681 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
    22741 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
    22801 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
    22861 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
    22921 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
    22981 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
    23041 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
    23101 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
    23161 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    23221 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    23281 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    23341 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    23401 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    23461 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    23521 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    23581 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    23641 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    23701 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    23761 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    23821 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    23881 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    23941 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    24001 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    24061 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    24121 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    24181 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    24241 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    24301 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    24361 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    24421 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    24481 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    24541 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    24601 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    24661 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    24721 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    24781 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    24841 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    24901 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    24961 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    25021 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    25081 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    25141 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    25201 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    25261 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    25321 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    25381 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    25441 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    25501 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    25561 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    25621 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    25681 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    25741 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    25801 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    25861 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    25921 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    25981 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    26041 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    26101 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    26161 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    26221 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    26281 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    26341 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    26401 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    26461 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    26521 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    26581 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    26641 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    26701 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    26761 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    26821 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    26881 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    26941 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    27001 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    27061 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    27121 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    27181 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    27241 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    27301 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    27361 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    27421 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    27481 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    27541 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    27601 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    27661 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    27721 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    27781 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    27841 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    27901 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    27961 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    28021 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    28081 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    28141 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    28201 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    28261 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    28321 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    28381 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    28441 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    28501 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    28561 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    28621 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    28681 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    28741 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    28801 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    28861 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    28921 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    28981 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    29041 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    29101 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    29161 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    29221 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    29281 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    29341 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    29401 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    29461 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    29521 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    29581 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    29641 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    29701 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    29761 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    29821 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    29881 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    29941 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    30001 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    30061 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    30121 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    30181 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    30241 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    30301 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    30361 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    30421 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    30481 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    30541 aaac
//
LOCUS       DACDHJ010000011        30216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDHJ010000011 DACDHJ010000000
VERSION     DACDHJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.011"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..204)
                     /locus_tag="IV223_000421"
     CDS             complement(<1..204)
                     /locus_tag="IV223_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710729.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
     gene            complement(192..821)
                     /locus_tag="IV223_000422"
     CDS             complement(192..821)
                     /locus_tag="IV223_000422"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8710730.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(828..1544)
                     /gene="pyrF"
                     /locus_tag="IV223_000423"
     CDS             complement(828..1544)
                     /gene="pyrF"
                     /locus_tag="IV223_000423"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8710731.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1541..2473)
                     /locus_tag="IV223_000424"
     CDS             complement(1541..2473)
                     /locus_tag="IV223_000424"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8710732.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2470..3246)
                     /locus_tag="IV223_000425"
     CDS             complement(2470..3246)
                     /locus_tag="IV223_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8710733.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3300..6485)
                     /gene="carB"
                     /locus_tag="IV223_000426"
     CDS             complement(3300..6485)
                     /gene="carB"
                     /locus_tag="IV223_000426"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8710734.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6490..7566)
                     /gene="carA"
                     /locus_tag="IV223_000427"
     CDS             complement(6490..7566)
                     /gene="carA"
                     /locus_tag="IV223_000427"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8710735.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7571..8851)
                     /locus_tag="IV223_000428"
     CDS             complement(7571..8851)
                     /locus_tag="IV223_000428"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8710736.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8870..9796)
                     /locus_tag="IV223_000429"
     CDS             complement(8870..9796)
                     /locus_tag="IV223_000429"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8710737.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9809..11092)
                     /locus_tag="IV223_000430"
     CDS             complement(9809..11092)
                     /locus_tag="IV223_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8710738.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11106..11645)
                     /gene="pyrR"
                     /locus_tag="IV223_000431"
     CDS             complement(11106..11645)
                     /gene="pyrR"
                     /locus_tag="IV223_000431"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8710739.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11880..12791)
                     /locus_tag="IV223_000432"
     CDS             complement(11880..12791)
                     /locus_tag="IV223_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8710740.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12788..13252)
                     /locus_tag="IV223_000433"
     CDS             complement(12788..13252)
                     /locus_tag="IV223_000433"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8710741.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13257..13955)
                     /locus_tag="IV223_000434"
     CDS             complement(13257..13955)
                     /locus_tag="IV223_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8710742.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13942..14328)
                     /locus_tag="IV223_000435"
     CDS             complement(13942..14328)
                     /locus_tag="IV223_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710743.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14475..16145)
                     /locus_tag="IV223_000436"
     CDS             complement(14475..16145)
                     /locus_tag="IV223_000436"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8710744.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16328..16561
                     /locus_tag="IV223_000437"
     CDS             16328..16561
                     /locus_tag="IV223_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8710745.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16660..17091)
                     /locus_tag="IV223_000438"
     CDS             complement(16660..17091)
                     /locus_tag="IV223_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8710746.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17170..17583
                     /locus_tag="IV223_000439"
     CDS             17170..17583
                     /locus_tag="IV223_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8710747.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17621..18769)
                     /locus_tag="IV223_000440"
     CDS             complement(17621..18769)
                     /locus_tag="IV223_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8710748.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18891..19487)
                     /locus_tag="IV223_000441"
     CDS             complement(18891..19487)
                     /locus_tag="IV223_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8710749.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19687..22329)
                     /gene="alaS"
                     /locus_tag="IV223_000442"
     CDS             complement(19687..22329)
                     /gene="alaS"
                     /locus_tag="IV223_000442"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8710750.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22717..23148
                     /locus_tag="IV223_000443"
     CDS             22717..23148
                     /locus_tag="IV223_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8710751.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23193..24542)
                     /locus_tag="IV223_000444"
     CDS             complement(23193..24542)
                     /locus_tag="IV223_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8710752.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24612..25775)
                     /locus_tag="IV223_000445"
     CDS             complement(24612..25775)
                     /locus_tag="IV223_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710753.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25904..26227
                     /locus_tag="IV223_000446"
     CDS             25904..26227
                     /locus_tag="IV223_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710754.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26410..27759)
                     /locus_tag="IV223_000447"
     CDS             complement(26410..27759)
                     /locus_tag="IV223_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8710755.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27828..28781)
                     /locus_tag="IV223_000448"
     CDS             complement(27828..28781)
                     /locus_tag="IV223_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8710756.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28883..30073)
                     /locus_tag="IV223_000449"
     CDS             complement(28883..30073)
                     /locus_tag="IV223_000449"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8710757.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8472 a         6644 c         5320 g         9780 t
ORIGIN      
        1 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
       61 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
      121 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
      181 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
      241 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
      301 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
      361 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
      421 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
      481 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
      541 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
      601 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
      661 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
      721 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
      781 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
      841 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
      901 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
      961 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     1021 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     1081 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     1141 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     1201 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     1261 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     1321 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     1381 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     1441 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     1501 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     1561 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     1621 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     1681 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     1741 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     1801 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     1861 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     1921 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     1981 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     2041 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     2101 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     2161 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     2221 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     2281 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     2341 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     2401 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     2461 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     2521 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     2581 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     2641 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     2701 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     2761 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     2821 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     2881 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     2941 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     3001 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     3061 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     3121 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     3181 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     3241 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     3301 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     3361 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     3421 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     3481 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     3541 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     3601 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     3661 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     3721 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     3781 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     3841 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     3901 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     3961 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     4021 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     4081 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     4141 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     4201 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     4261 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     4321 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     4381 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     4441 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     4501 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     4561 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     4621 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     4681 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     4741 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     4801 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     4861 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     4921 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     4981 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     5041 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     5101 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     5161 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     5221 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     5281 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     5341 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     5401 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     5461 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     5521 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
     5581 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
     5641 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
     5701 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
     5761 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
     5821 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
     5881 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
     5941 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
     6001 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
     6061 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
     6121 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
     6181 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
     6241 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
     6301 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
     6361 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
     6421 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
     6481 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
     6541 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
     6601 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
     6661 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
     6721 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
     6781 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
     6841 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
     6901 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
     6961 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
     7021 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
     7081 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
     7141 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
     7201 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
     7261 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
     7321 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
     7381 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
     7441 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
     7501 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
     7561 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
     7621 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
     7681 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
     7741 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
     7801 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
     7861 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
     7921 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
     7981 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
     8041 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
     8101 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
     8161 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
     8221 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
     8281 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
     8341 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
     8401 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
     8461 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
     8521 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
     8581 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
     8641 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
     8701 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
     8761 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
     8821 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
     8881 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
     8941 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
     9001 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
     9061 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
     9121 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
     9181 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
     9241 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
     9301 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
     9361 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
     9421 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
     9481 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
     9541 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
     9601 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
     9661 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
     9721 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
     9781 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
     9841 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
     9901 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
     9961 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    10021 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    10081 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    10141 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    10201 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    10261 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    10321 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    10381 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    10441 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    10501 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    10561 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    10621 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    10681 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    10741 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    10801 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    10861 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    10921 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    10981 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    11041 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    11101 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    11161 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    11221 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    11281 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    11341 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    11401 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    11461 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    11521 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    11581 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    11641 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    11701 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    11761 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    11821 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    11881 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    11941 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    12001 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    12061 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    12121 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    12181 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    12241 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    12301 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    12361 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    12421 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    12481 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    12541 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    12601 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    12661 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    12721 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    12781 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    12841 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    12901 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    12961 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    13021 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    13081 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    13141 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    13201 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    13261 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    13321 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    13381 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    13441 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    13501 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    13561 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    13621 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    13681 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    13741 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    13801 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    13861 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    13921 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    13981 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    14041 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    14101 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    14161 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    14221 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    14281 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    14341 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    14401 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    14461 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    14521 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    14581 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    14641 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    14701 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    14761 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    14821 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    14881 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    14941 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    15001 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    15061 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    15121 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    15181 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    15241 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    15301 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    15361 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    15421 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    15481 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    15541 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    15601 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    15661 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    15721 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    15781 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    15841 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    15901 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    15961 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    16021 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    16081 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    16141 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    16201 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    16261 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    16321 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    16381 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    16441 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    16501 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    16561 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    16621 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    16681 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    16741 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    16801 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    16861 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    16921 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    16981 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    17041 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    17101 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    17161 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    17221 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    17281 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    17341 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    17401 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    17461 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    17521 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    17581 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    17641 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    17701 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    17761 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    17821 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    17881 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    17941 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    18001 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    18061 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    18121 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    18181 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    18241 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    18301 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    18361 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    18421 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    18481 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    18541 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    18601 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    18661 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    18721 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    18781 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    18841 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    18901 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    18961 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    19021 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    19081 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    19141 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    19201 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    19261 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    19321 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    19381 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    19441 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    19501 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    19561 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    19621 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    19681 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    19741 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    19801 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    19861 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    19921 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    19981 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    20041 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    20101 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    20161 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    20221 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    20281 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    20341 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    20401 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    20461 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    20521 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    20581 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    20641 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    20701 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    20761 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    20821 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    20881 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    20941 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    21001 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    21061 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    21121 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    21181 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    21241 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    21301 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    21361 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    21421 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    21481 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    21541 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    21601 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    21661 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    21721 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    21781 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    21841 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    21901 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    21961 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    22021 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    22081 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    22141 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    22201 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    22261 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    22321 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    22381 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    22441 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    22501 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    22561 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    22621 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    22681 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    22741 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    22801 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    22861 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    22921 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    22981 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    23041 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    23101 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    23161 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    23221 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    23281 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    23341 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    23401 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    23461 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    23521 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    23581 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    23641 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    23701 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    23761 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    23821 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    23881 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    23941 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    24001 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    24061 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    24121 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    24181 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    24241 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    24301 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    24361 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    24421 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    24481 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    24541 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    24601 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    24661 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    24721 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    24781 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    24841 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    24901 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    24961 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    25021 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    25081 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    25141 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    25201 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    25261 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    25321 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    25381 aaataatatc ttaaataaat gccaaatggt atgagtaata aaacattaaa agctggttcc
    25441 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    25501 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    25561 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    25621 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    25681 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    25741 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    25801 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    25861 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    25921 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    25981 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    26041 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    26101 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    26161 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    26221 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    26281 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    26341 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    26401 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    26461 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    26521 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtctaaacaa
    26581 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    26641 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    26701 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    26761 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    26821 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    26881 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    26941 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    27001 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    27061 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    27121 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    27181 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    27241 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    27301 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    27361 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    27421 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    27481 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    27541 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    27601 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    27661 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    27721 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    27781 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    27841 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    27901 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    27961 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    28021 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    28081 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    28141 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    28201 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    28261 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    28321 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    28381 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    28441 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    28501 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    28561 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    28621 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    28681 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    28741 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    28801 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    28861 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    28921 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    28981 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    29041 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    29101 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    29161 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    29221 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    29281 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    29341 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    29401 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    29461 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    29521 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    29581 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    29641 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    29701 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    29761 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    29821 aaccattgat cgttcggact gctcccgctc ctacaccata agtttcggtt gcttcaatac
    29881 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    29941 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    30001 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    30061 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    30121 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    30181 atgagaatta tttaaccaca ttttttattt acagta
//
LOCUS       DACDHJ010000012        29461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDHJ010000012 DACDHJ010000000
VERSION     DACDHJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.012"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..920
                     /locus_tag="IV223_000450"
     CDS             51..920
                     /locus_tag="IV223_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8710758.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1085..2356
                     /locus_tag="IV223_000451"
     CDS             1085..2356
                     /locus_tag="IV223_000451"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8710759.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVICIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2353..3648
                     /locus_tag="IV223_000452"
     CDS             2353..3648
                     /locus_tag="IV223_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8710760.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3747..4010
                     /locus_tag="IV223_000453"
     CDS             3747..4010
                     /locus_tag="IV223_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8710761.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4455..4904
                     /locus_tag="IV223_000454"
     CDS             4455..4904
                     /locus_tag="IV223_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8710762.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4961..6379
                     /locus_tag="IV223_000455"
     CDS             4961..6379
                     /locus_tag="IV223_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8710763.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6379..7239
                     /locus_tag="IV223_000456"
     CDS             6379..7239
                     /locus_tag="IV223_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8710764.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7413..8186
                     /locus_tag="IV223_000457"
     CDS             7413..8186
                     /locus_tag="IV223_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8710765.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8333..8698
                     /locus_tag="IV223_000458"
     CDS             8333..8698
                     /locus_tag="IV223_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8710766.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8846..10054)
                     /locus_tag="IV223_000459"
     CDS             complement(8846..10054)
                     /locus_tag="IV223_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8710767.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10073..10351)
                     /locus_tag="IV223_000460"
     CDS             complement(10073..10351)
                     /locus_tag="IV223_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710768.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10544..11200
                     /locus_tag="IV223_000461"
     CDS             10544..11200
                     /locus_tag="IV223_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8710769.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11642..12439)
                     /locus_tag="IV223_000462"
     CDS             complement(11642..12439)
                     /locus_tag="IV223_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8710770.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12436..13620)
                     /locus_tag="IV223_000463"
     CDS             complement(12436..13620)
                     /locus_tag="IV223_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8710771.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13645..14892)
                     /locus_tag="IV223_000464"
     CDS             complement(13645..14892)
                     /locus_tag="IV223_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8710772.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15107..16852
                     /gene="dnaX"
                     /locus_tag="IV223_000465"
     CDS             15107..16852
                     /gene="dnaX"
                     /locus_tag="IV223_000465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8710773.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16872..17186
                     /locus_tag="IV223_000466"
     CDS             16872..17186
                     /locus_tag="IV223_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8710774.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17211..17807
                     /gene="recR"
                     /locus_tag="IV223_000467"
     CDS             17211..17807
                     /gene="recR"
                     /locus_tag="IV223_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8710775.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17871..18515
                     /locus_tag="IV223_000468"
     CDS             17871..18515
                     /locus_tag="IV223_000468"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8710776.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18530..18859
                     /locus_tag="IV223_000469"
     CDS             18530..18859
                     /locus_tag="IV223_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710777.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18873..19811
                     /gene="holB"
                     /locus_tag="IV223_000470"
     CDS             18873..19811
                     /gene="holB"
                     /locus_tag="IV223_000470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8710778.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19847..20671
                     /locus_tag="IV223_000471"
     CDS             19847..20671
                     /locus_tag="IV223_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8710779.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20664..21011
                     /locus_tag="IV223_000472"
     CDS             20664..21011
                     /locus_tag="IV223_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8710780.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21080..21952
                     /gene="rsmI"
                     /locus_tag="IV223_000473"
     CDS             21080..21952
                     /gene="rsmI"
                     /locus_tag="IV223_000473"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8710781.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22061..23182
                     /gene="dinB"
                     /locus_tag="IV223_000474"
     CDS             22061..23182
                     /gene="dinB"
                     /locus_tag="IV223_000474"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8710782.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23236..23838)
                     /gene="nrdG"
                     /locus_tag="IV223_000475"
     CDS             complement(23236..23838)
                     /gene="nrdG"
                     /locus_tag="IV223_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8710783.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24153..26342)
                     /gene="nrdD"
                     /locus_tag="IV223_000476"
     CDS             complement(24153..26342)
                     /gene="nrdD"
                     /locus_tag="IV223_000476"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8710784.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26668..27435
                     /locus_tag="IV223_000477"
     CDS             26668..27435
                     /locus_tag="IV223_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710785.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27425..>29461
                     /locus_tag="IV223_000478"
     CDS             27425..>29461
                     /locus_tag="IV223_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710786.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIE"
BASE COUNT         9436 a         5305 c         6168 g         8552 t
ORIGIN      
        1 agaattgtct taacaaattt agttttacaa aacttaggag gaattttatt atgccagtag
       61 tatcaggagc tgaattttta aaagctgctc gtaaaggcgg ttacgctgtc ggcggattca
      121 acacaaacaa cttggaatgg actcaagcaa tccttgaagc ggctgaagca aaacaagcac
      181 cagtacttat ccaaacatca atgggtgcag ctaaatacat gggtggttac aaaatctgta
      241 aagatttaat cactgattta gttgaagcaa tgaacatcac tgttccagtt gctatccact
      301 tagaccatgg tgattacgaa gctgctttgg aatgtatcga attaggctat acttcagtaa
      361 tgttcgatgg ttcacatcta ccatttgaag aaaacttgaa attagctaaa gacgttgttg
      421 aaaaagcaca tgcaaaaggt atctcagttg aatgtgaagt tggttcaatc ggcggagaag
      481 aagacggaat catcggtaac ggcgaattag ctgatattga agaatgcaaa caaatggttg
      541 ctacaggtat cgactactta gcttgcggta tcggtaacat ccatggtcaa tatccagaaa
      601 actggaaagg attagctttt gaccacttac aagcaattgc tgatgctgta ggtccagaca
      661 tgcctttagt attacatggc ggatcaggta ttcctcaaga tcaaatcgaa aaagctatct
      721 caatgggtat ctcaaaagta aatgttaaca ctgaattcca attatcattt gctgcagcta
      781 ctcgtaaata tatcgaagct ggtaaagata aagaaggtaa aggattcgac cctcgtaaat
      841 tattagctcc aggtaaagca gcaatcgttg aagatgctaa atctcatatc gactggttcg
      901 gttcagctaa caaagcgtaa gcatccacat aacagatacg atatatagga atggagaacg
      961 acttttgaag tcgctctcca ttctttttta cttaaaacgg cagaccatgc tcgtttcttt
     1021 tttttatcta tgataaaata caagcatatc catgaaaaaa gattaataaa caaggtggaa
     1081 acagatgaaa aaaatcgtaa tcaaaggaaa ccgaccttta gctggtgaag taacgatcag
     1141 cggtgcaaaa aatagtgcag tcgcattgat tccagctgca atcttagctg attccccagt
     1201 gatcttggaa ggtgtgccgg acatacaaga tgttcattca ttaattgaaa ttttagagat
     1261 catgggcgca actgtccatt tttccaacaa catactagaa atcgatcctc gagaagtcgt
     1321 atcgattccg atgccgcacg gaaaaatcaa tagtttgcgt gcttcttatt attttatggg
     1381 aacactgctt ggtaaatttg gcgaagcggt agtaggcctg ccaggtggct gttatttagg
     1441 ccctagaccg attgacctac acgtcaaagg atttgaagct ctaggtgctg aagtgacaaa
     1501 tgaacatggt gccatgtatt tacggacaga aaataaagga ttgcgtggca atcggatttt
     1561 catggacgtg gtttcagtcg gagcaacgat caatgtgatg ttagctgctg taaaagcaaa
     1621 aggacagaca gtaattgaaa acgctgcacg agaaccggaa atcatagatg tggcaacttt
     1681 actgaataat atgggggcaa aagttcgagg agcgggtaca gatgtgatct gtatcgaagg
     1741 agtcgaaaca ttacatggct gtcggcattt catgattcct gaccgcatcg aagcaggaac
     1801 ctatctagca ttagcagctg cagtaggaaa tggtatcaaa gtgaaaaatg tgatctttga
     1861 acatttggaa agctttatag caaaattgca ggaaattggt gtccatatga aaatcagcga
     1921 agatgaaatc gaagtttacc cctcgaaaga attaaaaccc gcaaacatca tgacctatcc
     1981 ttatccagga tttgccacag atctgcagca gccacttact gcgttactat tgatgactac
     2041 aggcacttca gaaatcatcg atacgatcta tgcgaaacgt gtgaaccatg tgccggaact
     2101 agcacgaatg ggcgctgata ttaccgtgga aggaaatatg atcatcgtga atggtccaaa
     2161 taaacttcac ggcacagaag tcgtggcttc tgatcttcgg gcaggtgctt gtttagtgat
     2221 tgctggacta ttggcagaag ggacaacgac gatttacaat gtagattata tcttacgcgg
     2281 ttatgatcat attatcgaga aactaacagc cttaggggca tcaattgaaa tgatagagga
     2341 agtgggagct gaatgagcga ttatttgaca atggcagaac tagagaacaa aacgttaaaa
     2401 gaagtttata gttatgccaa agaatttaaa attccttatt acagtaagat gaataaaaag
     2461 gaattatctc tagctgtgat tcgtgcacaa gcagagaaac aaggatttta ttatatggaa
     2521 ggaatattgg atatcgttgg tcaagatggt tacggttttt tacggccaat caattatgga
     2581 ccaagtgcag aagatatcta tatttctgcc tcacaaatca gacgattcag tttgagaaat
     2641 ggggacaaag tggcaggaaa agcccgtcca cctaaagaat cggaacgtta ttatggtttg
     2701 atgcacgtgg aaagcgttaa tggaaaagat ccggaagaag caaaagaacg tccgcatttt
     2761 ccagccttaa cacctcttta tccgcaaaga cagcttcaac tggaaacaac accgcaaaga
     2821 ctatctaccc gaatgatcga tatcttttct ccaatcggtt ttggtcagcg tggattgatc
     2881 gtcgcaccgc caaaagcagg gaaaacttct gtattaaaag aaatcgcaaa tggtatcacc
     2941 gaaaactatc cggaagtaga attaatcgtt ctactgattg atgaacgacc ggaagaagta
     3001 acggatctag aacgaagcgt gaaaggcgac gtggtatctt ctacatttga tctgcagccg
     3061 caaaaccata ctcgagtttc tgaattagta ttggaacgcg caatgcgttt agtcgaagat
     3121 aaacgagatg tcgttatctt gatggatagt attactagac ttgctcgggc ctataactta
     3181 gtcgttccac caagcggacg tacattgagt ggcgggattg acccagccgc attgtataaa
     3241 cctaagaaat ttttcggggc agcaagaaac atcgaagaag gtggcagttt gacgatttta
     3301 gcgaccgctt tagtcgatac aggaagccgt atggatgacg tgatctatga agaattcaaa
     3361 ggaactggaa atctagaatt acaactttca cgtgaattat ctgaacgtcg gatcttccca
     3421 gccatcgata tcaaaaaatc aggtacccga aaagaagaat tattgatgcc aagtgaagaa
     3481 ttagaagaaa tttggaaact tcgaaaaaat atgactggag attcattaga atatacggaa
     3541 caactgatcc gctttttgcg taaaacaaaa aacaatcagc aatttttcaa agaatttcaa
     3601 gatgtctcct ttggtaaaaa aaatccgacc agaaatctga aaagataatt gcaaccttga
     3661 ctgaaacatg ttaagatgtt aatgttgtaa atcgacaaat caactctggt tcagcaaatg
     3721 gagcagggca aaggagcgaa aaaatcatga aagaaaacat ccatccagat taccacccag
     3781 tagtattcat ggattcaaca actggtttca aattcttgtc aggttcaaca aaaacttcac
     3841 aagaaacagt tgaatgggaa gacggtaata catacccagt catccgtgtc gaagtaactt
     3901 ctgactcaca tccattctac actggacgtc aaaaattcac acaagcagac ggacgtgtgg
     3961 accgtttcaa caaaaaatac ggtctcaaag acgcaaacgc tgcggaataa taaaaacgct
     4021 gataactcaa cgtttagcag actatctgat agggatagtc tgttttttta tgttttttgc
     4081 caatttaaaa cttcgtttga tgctgaaaaa gtcgtaggat cataatgcac aaaacaatga
     4141 aaaactagta tatagtttta attatccact tgataaaaac accttatatt aacgaaatga
     4201 agaggggtta tttcgaagta aaaaaccatt tacctaaaat tagaagctaa gctttccttt
     4261 caaggaaggc tttttgtttt gttatagact tgcgattgtt tgtgagctag atcgttcatt
     4321 ttaatgactt atacacgcgg atgatgggtc tttttcctct gttcattcat aatattgaac
     4381 ggtacagcta gcgatgctat actgacctca gttccaagaa aacgttaaca ttttttgtgg
     4441 gggagaggtt ttttatgagg tttgatcaat tgatcgcaga agaactgatt gttttcgatc
     4501 catcgataga ttcaaaagga tcattatttg agcgaatagg ggatgtgttg aaaaaaacaa
     4561 ataaggtaag aaatgttcaa aaagtcgtta aggcactatt caaacgagag gaggagatct
     4621 ccacaggaat agaagatgga tttgggattc cccatgcaaa aagcaaatat gtgacaaaac
     4681 cagcactgtg tttttttcat acagccaaat taacggacta tagcgggata gatgaaatgc
     4741 caattgaata tatttttgcc atcgtagttc ctgaaaaatc agcagatatg catctagaga
     4801 ttctcagcac actctccaga cgattgatga atgcagattt tagaaaggga ataaaagaag
     4861 ccactacgcc agcagatatc atcagacaga ttagtcaaga atgaaaatag tagagtcttt
     4921 gtccgagtat cgatagtgaa gaaaaaggag gaaaataatc atgaaaatcg ttggtgtaac
     4981 tgcttgcccg actggaattg ctcatactta tatgtcggca gaaaaattag aagtaacagc
     5041 aagaaatcta aaccatcaag caaaattcga aacacaaggt gtaaaaacag aaaatcagtt
     5101 atctgaacaa gaaatcaaag aggcagatgc aatcattctt gcagtagata aagaaatcga
     5161 attggaccga tttgccggga aaaaagtaaa gcgtgtctct acaagccgag caatcaaaga
     5221 accgcaagta gtaatagacg aagcattgag agatattggc gtattcgttg tgtctaatga
     5281 aaaagaacca gctgcaaatg agaaaaagcc gacaatctat aatcatttca tgaatggtgt
     5341 gaattacatg cttccatttg tgatcgcagg cggaatcatc atcgcactga gttttgcctt
     5401 tgggattaca gcggctgatc ccaactcagc agactataat gtgttagcag ctgcattttc
     5461 aaggatcgga ggagatacag cgtttgccat gatggttcct gcgttaggcg cggggattgc
     5521 tacatcgatt gctggaaaag caggttttgc cccaggaatt gttgccggat tactggcctc
     5581 tacaggtggt tcaggttttc ttggcggaat gattggtggc gtgctagcag ggtatatctg
     5641 tgattttctg gcaaataaaa ttagcgtaaa aaaagaattt tctgctattt atcagctgat
     5701 tgtcgtcccc tttttatcga tcttaattat tgggcttttg atggtatttg tgattgaaca
     5761 accgatcaca tgggtgttgg atgctttgac aaattggctg aatagcttgg gaaatacatc
     5821 cgggctattg ttcggattac ttatcgggat catgatggca gctgatatgg gtgggccgat
     5881 caataaatcg acttcgactt tctcgatcgg tttgatgtca gctggagtaa atgcaccgat
     5941 tgctgcttgt atggctgcag gaatgacacc tcccctaggt attgctttag ccactttatt
     6001 attcaaacat aaatttacca aagaagaacg gacttcaggt caatcatgct ggattttagg
     6061 cgcttcgtat attacagaag gtgctatacc gtttgcggta tctgacccga tccgcgtgat
     6121 acctagtttg atgacaggtt cagcagtcgc tgcagggatt tccatggcag taggagttac
     6181 ttcaatggca cctcatggag ggatttgggt gatgctaatt ccaaatgtta tgaacaattt
     6241 acctatgtat gtcttggcaa ttgcatcagg tacagtcact acagccttta tgcttggact
     6301 attgaaaaaa acagtgaaag agaaaaaaca agacaagtgg acgaaaaatg aaaatctaac
     6361 aaatgaggtg gaagcgtaat gttatataca atgaaagagt tattgaaagt ggctaaagag
     6421 aatcaatttg cagtaccagc cttcaatatt tgcagctatg atatgatgaa agcaatcatc
     6481 gaagaagcag agagattaga agcaccagta atcttagaaa ttcatcctga tgaaatcgcc
     6541 tatttaagtg atgagtttgt tgcggcagta agagcctatg cttatacaag taaactgccg
     6601 atcgttatcc acttggatca tggcggtacg atagaagatg tcttacgggc gattcgaaac
     6661 ggctattctt ccgtgatgat cgatgcgtct ttgaaatcat tcgacgacaa tgtacagatt
     6721 acgaaaaaag tggttgcgtt agctcatgcg gtaaatgtct cagtggaggc tgagcttgga
     6781 acgattggga ataatggttc tgctgaagga ggtgctgaaa caatcattta tacagatcct
     6841 gatcaagcga agatttttgt gaaagaaaca ggaatcgata cacttgctgt agcaattgga
     6901 acagcgcatg gcttgtatcc gaaaaacaga actccaaaat taaatatcga cttactgaaa
     6961 gaattgaacc aacaaatcga tcttccattt gttttacatg gcggttcagg taatccggac
     7021 gcagaagtca gcgaagcagt aaaatacgga gttgccaaag tgaatttgag ctcagatctt
     7081 aaaagcgtct ttttcaatgc tttgagagaa atattgagtg aaaatccatc gatgtacgag
     7141 ccgaatgcaa tttatccagc agctaataaa aaggtacaag aagtggtcaa acataaaatg
     7201 gaaattttga acacagtagg caaggctagt ttatactaaa aataaaaatg tgtggtcaag
     7261 agggtatgac aatagtcttg tcataccctc ttaaccacac acgatgttca gaagtagttt
     7321 tttcgaaaaa tttcttctta tttctcgaag ccaatcactt ctgtcacaac ctcttttttc
     7381 ctgttactat ataaagtaaa tggggtgatt ttatgttgaa tgaagtggag aatagacagc
     7441 aggagattgt gaattatcta aaaaacaacc aatttgcaag agtcaccgat ttgatcgagt
     7501 tagtaaatta tagcgaagcc acgattaaac gtgaccttgt ttttctggaa aaaagtggat
     7561 tgatccgtag agttcgcggt ggcgccatgc ttattgacaa tcgaaaaatt gacgtgcctt
     7621 acctgatgaa gatcaccaaa ttagaagaag aaacagaaaa acgtaagatt gcagatacag
     7681 cttcttcttt gattcgagat gatatggtct tattcttgga ttccagtaca acctctttac
     7741 acttagtcag aaacctagca agatttgatg gtttgcagat cattacgaat ggcatcgtca
     7801 cagctgctat gttgagtgaa tatacaacgg caaaagtatc tgtattggga ggggcaattg
     7861 ttccgaaacg agcaacaatc aacggttcaa aagcctataa cgatgcattg tcatattatg
     7921 cggatttagc gataatgagt tgtcgcgggc tagatttcag ccaaggagta acagaaactc
     7981 atgaaggaga ggcaatggtc aaaaaagctt ttagaaaaca atcagaccgg cttgccgttt
     8041 tagttacaca gcaaaaaatt gcacataaat tcatgcatca agccattgct tttcatgaaa
     8101 tcgattatct ggtgacaagt aaagaactga caaaagaaca gtatgcgaaa gtaaaggaac
     8161 accatatcga atgtctgtac ggataaatta ttacgtgaat gttctaagtg gcttcctcat
     8221 ttttagatag gaccttatct ttgctcgctt ctctcagccg atggtataat aatgaaaagg
     8281 ttatcttgtt agataatctt acttttttat ccaggagaaa ggagtgcaga aaatgaaaca
     8341 tgtgaaggcg ttggtagtga aggcaatcat gatctgggcg attctgtggg tcgtgctgac
     8401 aggtttatac ggtgttagct ttatggattc aacgattgtt ggggtaatta ttgtagtcat
     8461 gatctatgtg ctaggggatc tgttgatctt acgtaaagta gggaacatcg cagcgacgat
     8521 cgcagatgct ggttcagctg cagttatctt gtggctctat ctatatttta tgaatgatac
     8581 agtcgatatt tggatgaaag tattgattcc tgctctattg atcggtgtgg cagaatggtt
     8641 cttccacaag tggttgctaa gtcaaggcat cgtaccagat gaacggaaaa tggaataaat
     8701 caacagttaa actattctta tcgataaatg aaagctttaa aaataaatag atgtttaatt
     8761 aagaataatc ataaactatt tttactcaaa aaaccgctca gattattttc agatctgaac
     8821 ggtttttttt atctattcat caagatcaaa tttcattttt caatgattca atcaattcgc
     8881 cctctgcttc atgactatgt tttcggccat taacaagata gagtgctgta catatcaatg
     8941 gaatgactcc ggcaatcaca tacaatcctc taaatgaagt aagagatttt agcatcccca
     9001 tcaaataagg accaacgcct aagcctaggt ctaaaccaat aaagtaggta gataaagcaa
     9061 tactgattcg atgttcgcgg acgattttta aacaaacagc ctgtccattt gacataaacg
     9121 taccataacc taggccgact aatgctccag agataagcaa tgtcacacta ttcgtcgtta
     9181 cactcagtaa aaataatcct gctgttaaga ataaatagct aggatacatc acgtattgtt
     9241 ctcctttgac atcgaaaatc cttccagtca atggacgtgt aaatgtgatg accaaagcat
     9301 atacgacgaa gaaaaatgac ccagcagcaa ctaagtggat tacttttgta taagaagata
     9361 aaaatgataa aacacttgaa taagaaagac ccatcaaaaa ggcaattcct gtaatgaaaa
     9421 gtgctttctt ttcaataaaa ctattgatat tccatgagcg aagagatatt ttttgttctt
     9481 cagataatgg aatatttttt acaggaaaga caaggcaggc aatcgtaaca aagaagacca
     9541 gtacaattga aaaccagata atgaaacgaa aatcagtcat attcagcaaa atcattccta
     9601 aaaatggccc aacagcggca gctaaactag tacttaatcc ataataattg atgccttccc
     9661 cttttttaga tgcagggata tatgcagtaa cgatagcatt tgtagctgta gaaactgtac
     9721 cgtagccgaa tccattcaag aatcgaacta aatacaatgc cccgatagtt ggcgtataaa
     9781 gatatgctaa tgttgaaagg agatagaaga tcgcgccgcc tcttaaggta aacttccgac
     9841 caaatagttc taactgtttc cccatgaata agcgtgccag caatgttccg atgatataga
     9901 tcccagaagc aaaacctgct tggctcaagc tggcgtgtaa ggtgtcttgc gcaatgactg
     9961 ccatgatgac catcaacaga taataaatca ggtagacaat aaaattgatc aatgtgattc
    10021 caataaaata tttattaaat aatttctttt ccatgtatgt tatacattcc ccttaagctg
    10081 aatatgcttt taatttttgt atttgaactt ctacatcgtg ataattggat ggatagcgat
    10141 caacaagttg gcgaataaat aaatctcttg tagacataaa cagatttaaa aattcttcgc
    10201 attcgtttgt tggattctca tccactgctt tgatcaattc gatcgagttt tgatacgcat
    10261 catttaaaaa gactaaagaa gctttcatat caaatcttgt ttgatatgct gtttctaatc
    10321 gattaatgat tgtgttcatc cagtaattca tgttttttcc ctctattctt aaaaaatttt
    10381 acttctgcag tataacgaat aaaaaaaagg aaaaaaatcg cataagtgat ggaaaaaggt
    10441 acttaacaaa tgttaatatc attagtaaaa tacctttgga ctttaaaaag ttttaaagta
    10501 ctgtatgatc tttttagatg aaagtttgga tgggggagca tcattggata gtagagtctt
    10561 aaaggaatat ttggataaaa atgattttcc gattatacgc aaaggatatc ataaatattt
    10621 gacattcgaa gggatggaag attcccatac gtatatcttg aaagagggaa cggtaaaagc
    10681 aagtgttatt tcaaatgatg ggagagaatt taaccttcgc tatatcaaag atcttgaaat
    10741 cgtctctctt ttaaaagacg aatattcaca attcatcgat tcgccgtata atattcgagt
    10801 tgaatcagaa tatgcagagt tttatcgaat caatcgtgtg cgcttttggc aaaatatcaa
    10861 ccaagaccca aagctacaac actatgtaaa agagtattac cgctatcgtc tgctttattc
    10921 gatgaaaaaa atgcagcaaa tgctattaaa cggaaagctg ggagctgttt gtacgcaact
    10981 ttacgaatta tatgatttat tcggtgtgcg gacagaagaa gggatgctga tcgatttttc
    11041 gatcacgaat gaagaaatcg ccaaattttg tggcatctca actgctagca gtgtaaacag
    11101 aattttaaaa caactgaaaa aagatggtat tatcaccgtt aaagaccatc atatcgttat
    11161 tttgaatatg gacttgttag aagacaatat tatcttttga tgaacaaaga ggttgtgaaa
    11221 tcagcgtttt gacctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc
    11281 gttttttgag ctaataccga gggtcagctg attgaacacc gtttaccaag aggttgtgag
    11341 cctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat
    11401 gaatattgtg acttgtgacc gaggagttgg taggagaaca ccgtttacca agaggttgtg
    11461 aaaaagctgt tctgcatcaa gtaataagaa gagccaagta aaaacagtct cttatgtttt
    11521 taacttggct ggacttatca ccgtagatgt aagcttttga acgccgttta tttgcaacaa
    11581 aaggggctgt gacacgattt ttgtcacagc cctttttttg cgaacattca tttagcaatt
    11641 tttagaaact tctgccgcca ccgccaaaag tcccgccacc gctagaatgg gtagtacttc
    11701 cgccaccgcc gcttccagga ggtgggcttt tggggattct tcttgtcgtg acaaaagaat
    11761 ttgtcagacg atcggttttt tcagttaatt tgatactgga tttttctcgg aatggatatt
    11821 tgtagatgcc tgatttcaat tgataacgcg aaacagtcac aaaataaaag acaagactaa
    11881 ggataagagc caaaacaaca gcaatgatga tttctactgt cgtcaaaacc ttatagcgtg
    11941 taattttgcc agtttcttcg tctacacgat aatgtcctcc tggaactccg tcttccacat
    12001 atgttgctga tttagacagg taagcttttg cagcattgta ataggcagca tttgtcatct
    12061 gatcataaac atcatctaag atcgaatcga ttctgctatc agtcaaataa tcgatcatat
    12121 taccagaagt cgaaatgtag atttcacgct gtcccatatc taatagcaat actgagccat
    12181 tttgatcatt tccgactgcg tctcttaagt aattatcagc aaagtctctt ggttcttctg
    12241 aattagatgt agtcgttacg atcaagactc gtcctttgat tttttcattc agcttttctg
    12301 cctgttgttc caaagattgg atttgatctg ttgtgaataa cccagcctca tcatttaccg
    12361 tagctgtatc tgcatgagta gccaccggcc agccaacact tactagcaaa agaaagaata
    12421 aaaaaaattt tttcatcata tcaagtaacc tccgatcaga aagaggatcg ctagcccagc
    12481 gaaaatccca agagcggtca gtcccaattt tttctggctg atcggcagga caccgctgac
    12541 ctttcctgtt tgtccattca ttgcgtagta gtagactttt ttacttgagt cattgcttcg
    12601 ataagtaaca agccataccg gcagtaatac gtaattattt acttcgtttg tgattgaagc
    12661 agaagttcgc acaccggtca atgttgtata tccatttgcg gtatctttta acagcttctc
    12721 tgaatagtct tttaattcag attgaatctg tgatttgatt gcctcatatt cgatatctct
    12781 tttttctgct tggaacccgg ctaaatactg gcttttaaaa tcaattgctt tatctaacgg
    12841 gaaaggttgc acgccttcga ccatcttttg ttgcgtattt tttgataagg cattttttac
    12901 tagttcttta aaagaaagct tgcctgctct tgaaacagca aattgcttcg tttctgtgta
    12961 ttcgatgtca cctatccgcc atatgcggat aactgtacca ttcgcctgca tggcaccatc
    13021 tacttcagca tctgtagccc aataagggaa atacacacct gtcaacttat cgatttgatc
    13081 cttattgaaa aaagctttgg gaacaaacca ttttttcttg gtccaagcga gaaatctgtt
    13141 gattgcttct tctttctcga cagcaaaagg aagtacttta tttggcaaga attctccgct
    13201 taaacgtccc gatagtacga cgggattatg gcagtagtaa caataagtcg cagcagttgt
    13261 cgcatcagtc acgatctctg caccacagtt gggacaaaga aaaagatcta tcgctgcggt
    13321 attatctgca gattcgtccg ctgtatctga aaaaccgcct gcctcgtcaa ccgctttgcg
    13381 ctcggcttca ttcatatcgg caagctgttc ttgggctgta aaagtaaaat cttcggtagg
    13441 tgaaggttct tctttgcttt cttcgccagc catacgcgct tctttttgct tttgctcata
    13501 ttggctgact tcttcttctg tatagatatt caggcagtat tcgcaatgaa atttttggtc
    13561 tttgggatca aaagtcaacg gaccgccgca attaggacat ttatgtgtaa gaacatccat
    13621 cagctgcaca cctttctaaa taaattaatt aacggggatt cctttaaacg gtttaccaca
    13681 gtttggacag aatttaggga tgccgttgga aagatccacg atctcattgc attcactgca
    13741 tcgcatttgt agcttaggct gaacgttttc tacaggtttg gcagctccgc agttggaaca
    13801 gaattttccg gaattttctg tcccgcaaac tggacatgtc caagtatccg catttccttg
    13861 tgcagtttgt tgattttgtt tttctgcttg ttgtttgatt tgttcttggt tattttgagc
    13921 tgcttgagaa agataagaac catttgcatt cattcccata cctacaccca taaagccggt
    13981 cattgctcct gcttcatttt ttccggctgc ttccatacca cgtgcaatgg aaccttgcac
    14041 gtatccttca cgtacgctag gatcacctag catcgcccct tcgttacgca tattgatcag
    14101 tttcacagaa tcatcggtgt aagaaatgct ggctacagcc acagaaacaa tctccatccc
    14161 acgtaattca cgccaagaat catccaatgc agtatccatg tatttgctta attccaaact
    14221 cttagatggt acataagaaa tccgttgacc atctgcagac atctgattga ttgctgattg
    14281 tagcgctgtt aggaattcag ctaaatactg ctcattgata tcattgattt ccacttgtgt
    14341 tttgttttta gggattgcgt ttgtataaaa taaaatcggg tcaacgacat gaatagaata
    14401 agtgccatgt gcccgtaaga ataattctgc gttatagaaa ttgtcaaagt agttcaacgg
    14461 cgcgcttgta ccgaatttga ttccctttat ttcttggaga ttgatataaa aaacttgttg
    14521 tttctgcggt gtgacgccgc caaatttgaa tcggtcaaaa gtttcagcaa ttgcttcttt
    14581 cagtgttcca ttgaacatgg aaggagctgc gtcattttta atggtgtaat aaccttcctc
    14641 agcggtataa tcgatgattt ttccaccatc gaccaaaagc atcatcatgt ttggatagac
    14701 atggacgact gttccgtctg ttaaaacatc ttctgttcct ttacgattgg agccacgttt
    14761 gtcatctttg cggactttga caccttttgt catgaccgtt gtatcactca tattgtctgg
    14821 ttcgatgact tctagccatt gatctgccag accgccgcca accatacttg ttgctgcttt
    14881 gataagaccc ataaaaaatt cctcctctga ttcagctagt gccaaacaat tattgtttat
    14941 ttattatacc ataggaagat gtgaaacttc tcggactaga gacggaattg aaatggaaaa
    15001 ttgcgtattg tttaaatacc tgaaaaagcc gagaaggata gtagtttttt tacctaatat
    15061 cacgtataat aagagatagt gaatttttta aaaaaggggg atccttatgg catatcaagc
    15121 actttatcga gtctggcgtt cgcagcgttt cgaagacatc gttggacaaa aagctgtgac
    15181 acagacacta aaaaatgcta ttgtttccca caaaacctca catgcctact tgtttacagg
    15241 acccagagga accggaaaga cgagtgcagc aaagattttc gcaaaagcaa tcaactgtcc
    15301 gaacagtaaa gacggtgagc catgcaacga atgcgaaatg tgtcgatcaa ttactgctgg
    15361 tacgcaagaa gatgtgatcg aaatcgatgc tgccagcaat aacggagtcg aagaaatccg
    15421 atttatccgt gatcgggcaa attatgcgcc tacaaaggca gcgtacaaag tgtacattat
    15481 tgatgaggta catatgcttt caacaggagc tttcaatgcc ttgttgaaaa cattggaaga
    15541 gccaaaagaa agtgtcatat tcatcttagc aacgaccgaa cctcacaaaa taccagctac
    15601 gatcatttca cggactcagc gatttgattt taaacggatc aatacggcag atattgtcga
    15661 acatcttgaa tttatcttga aagaatcgac tatcagctat gaaccagctg cattgaatgt
    15721 gattgcccgt tctgcagaag gcgggatgcg ggatgcattg agtattcttg atcaggcaat
    15781 ctcgtttagt gaaggaacaa ttactttaga tgatgccatg caagtgacag gtagcttaac
    15841 tgacgaaatg atggacagtt acttgagtgc ttgcgtgaaa aaagaagtac cagaagcatt
    15901 ggaaacattg aaccaaattc tcgcagccgg caaagaatca cgtcgctttc tagaggatat
    15961 gttacaatac tgccgtgact tgctgatgta tcagcaagca cctaacctag ttgaggaaca
    16021 atcgagtacg atcagaagta catttacaga attggcacaa actgtttcag cggatcagct
    16081 gttttcaatg ataaagattt tgagcgatac tcaaaatgaa attcgtttta ccaatagtgc
    16141 cgctatttat ttggaagtcg ctacagtcaa acttgcacag ctaccgtctg cttctgttcc
    16201 ttcagcagat gtagcatttg cagatgatca gatagtaaaa cagctacaac aagagattaa
    16261 ggaactaaga aacgaaatca aggaactacg cagtaataca ggaattgaga atacaaaagt
    16321 acagccggta cagcaaacaa atcataaaaa acagacaagt acctatcgtg taccgacgga
    16381 acgagtatat aaagttctgc gggaagcgac aagacagcat ctgaaccaag taaaagaagt
    16441 atgggaagac ttgctgatga gtctatctgt cactcagcga gcaatgctta aagcaagcga
    16501 accagttgca gctggaccta caggactagt gattgcgttt gattatgaga ttgtttgtca
    16561 aagagcagca aatgacgaag aattgcagct ttcggtacac aacaacttaa gccgaatgat
    16621 caaagattat gcgcctgatc ctgtttatat tacaagagaa agctggccag aacttcgaag
    16681 aaattatttg acgcaggcac aagaagaggg aacagaaaac tcttttgctt ctgaagactc
    16741 agaaggagaa atcgagttgc ttcctcaaga acaagaagta ccagaagaaa cacttgtagt
    16801 aaaagcagag gaactgtttg gctccgaagc tgttacagtg gtagatgatt aataaaaagg
    16861 agtagataat tatgatgcgt ggaatgggca atatgcaagg catgatgaaa caagtacaaa
    16921 aaatgcaaaa agaaatggca gaagcacaag agctgctaaa taaaacagag tttacaggta
    16981 aagccaccaa tgaattggtc aaagcggttt ttacaggcga tcgtcgcatg aaagatatcc
    17041 aaatcaatga agaagtcgtt gatcctgatg atatcgagat gcttcaggat cttgtcgtaa
    17101 tggcagtcaa tgatgcgatc gcacaaattg atgtggaaac tgagaaaaaa ttagggaaat
    17161 acgcaaaagg tttgccagga ttttagtttt aaactgaagg agtaatgaat atgcaatatc
    17221 cagaaccaat tgcgaaactg atcgacagct acatgaaact tccaggaatc ggtcagaaaa
    17281 cagcgacaag actcgccttt tatacgatcg atatgaaaga tgaagtagta aatgaatttg
    17341 ctaaatcttt gttaagtgta aaacgcgatt tgcatttttg tagtatctgc ggaaatatca
    17401 cagaagaaga tccctgcgaa atatgtagaa atccttcacg tgatcgaagc gttatcctag
    17461 tggtcgaaga gccgaaagat gtaatggcaa tggaaaaaat gcgagaatat cacggacttt
    17521 atcatgttct ccatggtgtg ttatcaccga tggaagggac aggtccagaa gacatcaata
    17581 tttcttctct gttgcagcgg ctgcatgatg cagatgtaaa agaagtgatt atcgcaacaa
    17641 atgcaacaac agagggagaa gcaacagcta tgtatctctc acgtctgatc aaacccgcag
    17701 gtatcaaagt gactcgtcta gcacatggtc tgtctgtagg ctctgatatc gagtatgcag
    17761 atgaagttac tttattgaaa gctgtcgaag gaagacgtga gttataaatt tctaattaag
    17821 tgttttttca gatacaatat aaaagcagca caagagatgg gggcaacaat gtgaacggac
    17881 tatttattac aatcgagggg cctgatggtg caggaaaaac cagcatcttg aatgaacttt
    17941 accctctttt gaaagaagta gcaaaaacag aaatcattca aacaagagaa ccaggaggca
    18001 ttccgattgc tgaagagatc cgagcagtta tccttgatcc aaaaaatgat cggatggatg
    18061 aacgtacgga agcactgctt tatgcagcag caagaagaca acatctagtc gaaaaggttc
    18121 tgcctgctct ggcaaaaggg aaaatcgttt tatgcgatag atttgtcgac agttctcttg
    18181 cttatcaagg tgcaggccga cgcatcggcg ttaccgagat tgctcggttg aatgagtttg
    18241 ctacagaagg tactacgccg gattttaccc tttatcttga cgtagattca gacaccggat
    18301 taagaagaat caagaaaaac agacaaaatc aaatcgaccg tttagattcg gaaggtttgg
    18361 aattccacca gcgtgtacgt catgcttact taaagctggc agaagagaat ccggaaagaa
    18421 tccacaaagt tgatgcaaga aaaagctttg aagaagtgct gcaaacaagt tatcatacaa
    18481 ttattgaaca gtatccgcaa ttttttgaaa actaggaagg tgaacgttta tgaaaattat
    18541 tttagcgatt atccaagaca aagacagcaa ccgtttatca aatgaattga ttgatgcaaa
    18601 tatccgtgcc acaaaactat catcgacagg cggcttcctt aaggctggaa acagtacgtt
    18661 tatcgtgggt attgaagatg accgtgtaga agaagcattg gaaatcatca aaaaaacgtg
    18721 tgaatcaaga aagcaatttg tatctacacc tgttacactg gatatttcga tggatggggg
    18781 cgtcccttat ccagtagaag tagaagtagg cggtgccaca gtcttcgtct tacctgttga
    18841 aggattccat caattttagg aggagctgag ctatgaatca agaaataaac ttggctcaaa
    18901 tgcaaccgat cgtgtatcag cagcttcaac ggagttttga gcatgggcgt atcgctcatg
    18961 cttatctttt tgaaggggag aaaggaacag gaaaacatga agtaggtata tggcttgcac
    19021 agcacctgtt ctgtacccaa atgaaagaca atctgccttg tggaaaatgc aacaattgtc
    19081 agcggatcga aaagaaagaa catccagatg ttttggttat tgaaccggaa ggacaaacca
    19141 tcaaagtcga tcagatcaga cgtttgcaga cggaattcag tcgcagcggt tacgaatcaa
    19201 gaaaaaaagt ttttttgatc aaagaagctg aaaaaatgaa tagcagtgcg gcaaacagtt
    19261 tgttgaaatt cttggaagag ccccctggag attttttagc ggtgttagaa acagatgcag
    19321 taggaagaat tctccctact attcaatctc gttgtcaagt tctgcatttt caagaacttt
    19381 cgaaagaagc actgatccat cgattgcaag aagagcagat tccattggaa aaagcaaaac
    19441 ttttggcttt cttaacaaac agtttaggaa aagcagttga aatctctcaa gatgaatggt
    19501 ttaatgatgc taaggacttg atttatcaat ggttcgttta cttgcaaaag caagatactc
    19561 aagcctttat ctatgtccaa aaaaaattgg taaaatcatt taaagaaaaa acacaacagt
    19621 ttacaggatt atctgtcttg atgttttatt atcaggaagc acagcgtcaa gcagtagcaa
    19681 acggcgaata tggcaaagtg aaacagataa accagataat tgaacggata ttgatcgcag
    19741 agcagaaact acgcagcaat gtcagttttc aggctgttgc ggaacagttc gttttgcata
    19801 cgatttttta gcttgccgca aaggatgatg atcaaggagg atggaaatgg tagaagtagt
    19861 aggtgtccga tatagagatg caggtcatat ctattatttt gcaccaggag actctgaata
    19921 tgtttatagt gacaaggtat tagtcgaatc tcagcaatcc ttacatattg ctactgttgt
    19981 ccttcctaaa aaagagattg atcccaatga ccttcctgaa gctatccgtc cgatcttgca
    20041 taaagcgtca caagctgaat tagaaaaaga caaaaaaaac aaaaaagatg cttatgatgc
    20101 attgaaaatc gcaaaagaaa aagtgcagtt gcgtgagttg gaaatgaaaa tggtcaaagc
    20161 cgaatacaca tttgatcgaa ataaattgat tttttctttc acagcggacg ggcgaattga
    20221 ctttagagaa ttggtaaaag atctggccgc tgtatttcgt actcgtattg agcttcgaca
    20281 aattggtgtc cgcgatgaag caaagataat aggcggaatt ggtccatgtg ggcgtccatt
    20341 atgctgttct tcttttttag gcgatttcgt gcctgtatca atcaaaatgg caaaagatca
    20401 aagtttatct ttgaatccaa caaaaatttc cggtttatgc ggacgcttga tgtgctgtct
    20461 gcaatatgag agcgaagaat acgagacagc gaaaaaagaa ctccctgact ttggaaaagt
    20521 aattgctaca cctgatggta aagggaaagt cgtagggttg aatctgctta gccgtatcgt
    20581 tagtattcgg ctgactggac gggaaaatcc tgtcgaatat gaatgggatg aattgaaaga
    20641 atttatccaa gtaggtgaga tgaatggata agagatcatt atacgacgga ttgaatcaat
    20701 tagaatcaga gttgagaaat acattaacag aacttgttga aatgaagcat gctttgcatg
    20761 agatcgtaga aaaaaataca actttggaga tggaaaatca acgtctgcgg gaacacctgc
    20821 gggaattgaa ccaagttgca caagctccag ctgaaaatgt gaagcaggaa ctttctaagt
    20881 ctcgtatgaa tcttgaaaaa atttatgaag aaggattcca tgtctgctac gatttatatg
    20941 gatcaagaag ggacaatgac gagccttgtg cgttttgtct agaagtgatc tatcgcgaaa
    21001 gcggtcgatg accaataaca agaggagact ggagcggaga aatactgctc cagtcttttt
    21061 attaaggaga ttgtgacaga tgtataatca aaaaagtttc aagggaaatc cggtatatgg
    21121 ggatttatat cttgtaccga cgccaattgg aaatttagaa gatatgagct acagaagtgt
    21181 tcgtatgctg caagaatccg atcttatcgc aagtgaggat acgcgtaata cacaaaagtt
    21241 attgaatcat tttgaaatcc atacacccca aaaaagttta catgaacata attataaaga
    21301 acgtgtccct caattgattg ctttgttaaa agaaggaaaa acgattgctc aagtcagtga
    21361 cgcaggaatg ccttccatca gcgatcctgg acatgagcta gttttagcct gcataaaaga
    21421 agggatttct gtgatctcta tcccaggacc tacagctggg atgacggctt tgattgcttc
    21481 agggctagtg cctcaaccat ttttatttta tggcttcctt ccacgtaaga aaaaagaaca
    21541 gaaagaaaca ttgaaaaagc taaaagaaca gacagttacg ctgatttttt atgagtcacc
    21601 ttatcggatc actgctacat tgacgaattt cttagaaatt tttggaaatc gccaagtcgt
    21661 tttgtgtcgg gaattgacaa aggtccatga agaatacctt agaggaagta cggaagagct
    21721 actcgcttat atcgaagaac atccagtaaa gggtgaatgt tgtctgcttg tagaagggaa
    21781 tttgttcgaa atgccagtga cggaagttga tgaagagttg ggaacgttaa aggaacaagt
    21841 agaagcaaag atcgcttcag gcgaaaaacc aaatgctgct atcaaagcag tagcgctgaa
    21901 aaatgggctg aaaaaacaag aagtctaccg tcagtaccat gaattagact gagatagtat
    21961 aagttgtcta taatgaaaga ctaatgagga aatcagctac taacttttca aatctcttga
    22021 acgcgaacga atgttcttgt ataatgaata agaggtgagg atgatggagc tgcaatttcc
    22081 tttacataat gatacatcaa gaaaaatcat tcatattgat atggatgctt ttttcgcttc
    22141 ggttgaagaa cgagatcatc ctgagtttcg cggacatcca ttagtaattg ccagacaccc
    22201 cagtgacact ggtgggcgtg gagtagtgac tacagcaaat tatgaagcaa gaaaattcgg
    22261 aattcattca gcaatgagtg cacaaaaagc gtatgagctt tgtccgcaag ccatctttaa
    22321 gcctggcgac catcataaat acagcgatat ttccaaacaa gtacgggaag tattctatcg
    22381 ttatacagat attgtggaac cagttagtat cgatgaagcg tatcttgatg tgaccgaaaa
    22441 taaaatcaat tgcaagtcag ccatcaaaat cgcccgaatg atccaagcag atatttggga
    22501 gagtgtccag ctaacatgtt ctgcgggagt aagttacaat aagtttttgg ccaaattagc
    22561 atcagactat caaaaaccta gaggaatcac ggtagttgcc cctcaagacg ctgtgccatt
    22621 tttaaaagct ttgccaattg aaaaatttca tggggtaggg aagaaaactg tacctagaat
    22681 gcatgagcta gggatttata caggaaaaga cctatatgaa tgcacagaaa tgatgttgat
    22741 tcgaaatttt ggaaaaatgg gctattctct ttatcgtaaa gtcagaggaa tccatgattc
    22801 tccagtaaac gttacaagag agcgaaaatc tgtgggaaaa gagcatactt atgggcagcc
    22861 gttgcaaacg gaagaagcag tgctgactca gttgcgccaa ctagctgaaa aagtagaaga
    22921 agcactgaga agagtccaaa aacatgggaa aacagtagtt ttgaaggtca ggtacacgga
    22981 ctattctact gtgaccaaaa gagtgacatt gccagaatat atctataaaa aagaagcact
    23041 cttttatcaa gcaagcctta tatgggagga aatcttggga gtagaaaaag gaatccgatt
    23101 gttagggatt actttgacga atctggaccc gatgacttat gaaaacatcg tccttccatt
    23161 atgggaaaat caagaaatat aaaaacagct ttgagagttg aaaccggaaa tccgttttct
    23221 ctcccaaagc tgtttttata tcaagtcttg ctttttgatt tgttcatagg tttctgttgc
    23281 atccgtacat ttttcccaaa tcacaacttg attggattct aaagattttt ggacgtcaat
    23341 gatccgctgg ttgctacttc cgcgaaattg tagttttagg tcacgtttgc ttaattcaaa
    23401 gcgtccgtca acaaggatat cgatttgcga aaggagttct aatttatctt ctgaatcttg
    23461 tagcaattca tcaaaggtat agcctgtcca tgaccagata tcttttgtcg taccgaactc
    23521 ttttcggata cgtcgtacga cttgcagaca aacatctgta ttcaaaaaag gttcgccacc
    23581 aagtaaagtt aatccttgca cgtaatcgtt gcgcaagtca gcgatgattt gttcttctaa
    23641 ttcaggtgtg aatggtttgc catatcggaa attttgtgca gctttgttga agcagccttc
    23701 gcaggcaaat aagcagccac taacgtaaag actgttgcgg acaccttcac catcgacaaa
    23761 attaaacggt ttgtagtcag caatatagtt ttggctgtag tcttttgcta accattcttt
    23821 tggttttgga tttctcataa aaaaacttct ttcatttaag gttcaaagat atagctattg
    23881 aagataaaat ctactcgacc acataaataa gaacaaactg acctaaatcc attgttcaag
    23941 aaagcttgtt cttattttga tagagggcgt tcaaagatac aaagccatcg aaagagggtg
    24001 gtgaaaaaag acttgtcata gcctcttatt ctgaataaac ggtgttcaaa agcttgcatc
    24061 tgcagtaata agcccaacta aacgaaaaat atgaggaact atttttgttt agctattctt
    24121 attacttgat gcaggacagc tttttcacaa cctcatttca tgtgtttcac acgtgaggaa
    24181 atttctttat ggcggccatg taccatcgga cgagcttgcg gatttcctaa atagccacaa
    24241 gtacgtttga ctacatcaca tgtttttgga tcatgatttc cacattctgg acattcgaac
    24301 cctcgttctg tcggtttgaa atcaccttcg aaaccacatt gatagcaatg atcgattggc
    24361 gtattcgtac caagataccc gattttgtca tatgcaaaat cccatacagc ttctaaagct
    24421 ttcggatttt gttggagcat agggtattca cagtagtgga tgaatccacc tgaacaatat
    24481 tttggataat ccttctcaaa ttccaatttt tcaaatggcg ttgggttttt acgcacatca
    24541 tagtggaaac tgtttgtata atattcttta tctgtgatat tttcgatgat gccgaatttt
    24601 tctgtatcca aacggcagaa acggtcagtt agactttcac ttggagttga atagacactg
    24661 aaatgataac catattcatc cccccatgca tctgcattag cttttaattc tttgacgata
    24721 tcaagtgtga aagcttttgc ttctgggttt gtttcccatt cgccaccgaa gaaagctgaa
    24781 gcaacttcat acaatccaat gtagcctaac gatacagtag ctcgacggtt tttgaataat
    24841 tcattgacag aatcggtacg tgaaagacgt ttcccgaaag caccatacat atataaaatt
    24901 ggcgcatttg ctggaatggc ttctttacaa cgttctacac gatagagaag cgcatctttc
    24961 atgatttcta agcggtcaga cagtaactgc cagaatttat tctgatctcc ttttgattcg
    25021 atcgcgattc ttggcaaatt caatgtaaca acacctaaat tcattcgtcc gacatttact
    25081 tcttgaccat tttcgtcttt ccacccttgc aagaatgaac gacagcccat aggtactttg
    25141 aaacttccag ttaattcaac gattttatcg taattcagaa tatcaggata cattcttttc
    25201 gttgcacatt ccaatgctag ttgtttgatg tcgtaattag gatcttcagg gtttaagttc
    25261 acaccttttt tcaaagtgaa gatcagttta gggaagatag ctgtccgctt ttcactccct
    25321 aatccattga ttcgaatctg taaaattgct ttttgaattt ctctttcaaa ccagttttct
    25381 cctagtccaa atcctaatga agtgaaagga gtttgtccat ttgaagtaaa taaggtattg
    25441 atttcatatt ccaaactttg catagcatcg aagatatctt ttttggtttt tgcttttgca
    25501 tattcttttt ggcgttctgg cgaatcgatc cattcttccg cgtcttttag atgtttttta
    25561 tagttcaatt ctgcaaaagg agcaagcaat tcatctgttc ggtcggcaga acagccacca
    25621 tattgacttg acgctacatt ggcaataatc tgtgagattt gtgctgtggc ggtctggata
    25681 gattttggcg attcgacttc ggcattgccg attttgaacc cattgttcag catgcctttg
    25741 aaatcaatca agcagcaatt cgtcatcggt gtataaggat gataatccaa atcatggtaa
    25801 tggatatcgc ctttttgatg cgcatttgct atatgtggtg gcaacatttt cagtccgatt
    25861 gattttccaa cgatcccggc tgtcagatct cgctgtgtat tgaaaacatt actgtcttta
    25921 tttgcgtttt cgttcacgac agtttggtcc ttgttgatta gcttttcaat cgaaaagttg
    25981 atatctgttg ctttactgcg agcaaaatct cgtttggtac gataattgat gtactcttct
    26041 gctaattcat attcgttttt ttctagtaat atatgttcca cgatgttttg aatctcatag
    26101 attttgacat tttcagcaaa ccggcttttg atttcttgga caaccaagtc tgcaatcatt
    26161 tgaatttttt cataagtaag tggtgataat gagccgtgaa ttttcttttc tgctttgatc
    26221 agtgcttcat tgatcttttg ttcatcgaaa tcaacatggc ggccatcgcg tttgatgact
    26281 ttcatctggg atgtctctgg ctgctgagta acgacagttt tttcttcata atttactttc
    26341 attatcttca actcctgcta aattgttctt gtctatcata aaacaaaatc ggggaaggag
    26401 caactaaata tcggctatat atagtattca atgtgctgta taattggatt tctatactat
    26461 atgtagtgta tattttattg tgaaaaaagg tgaaaacttg tagctggtgg atttgttttt
    26521 tgcatattta attttgctga ataaaataat tgaataaaat tttttattca gtccaagtaa
    26581 aagtgacaaa aacataagag caaattattc gtagatatgc tactatgcaa ataaatagaa
    26641 atgaaaaatg aaagaggagg agaagaattg gaaaagatga tcgaagtgaa acatctaagt
    26701 aaaagctacg gcagcaaact ttctcccgtc aaagccttag ataatatctc attcacagta
    26761 gaagaaggcg agtttgttgg gattatgggc ccaagtggtg caggaaagac aacgctgatg
    26821 aatatcttgt ccacgatcaa tttgcctacg atgggcagtg tctcgatcca aggaaaagaa
    26881 atcacaaaaa tgaaaaacag tgagctcagt gactttcgtc gaaaaaaact aggattcatt
    26941 tttcaagagt tcaatttgat cgatacccta aatgcaaaag acaatatcct attgccccta
    27001 gcagttgaac ggatgactaa agaggaaatg gaaaagagag taagacatgt agctcaattg
    27061 ttgaatatag aagaactgct caaacggtat cccgatgaac tttctgtcgg acagagacaa
    27121 cgaattgcag ctgctagagc acttgttgtt cagccacaag tgatttttgc agatgaacca
    27181 actggctctt tggactcgaa gtcggctaca gaattgttga actacttgaa gaccatgaat
    27241 caaaaagaaa aagcaacgat actgttagtc acccatgatc catatacagc aagctactgc
    27301 gatagaattt tatttattaa agatggtgtg attttttctg aagttgtcag aagagggaca
    27361 agaagagagt tctttgagaa agtaatagac atgcaggcga cgattggcgg aggaggaaaa
    27421 atgaatgctg tataaactag ccgttaaaag tttttttcgg cagtcacgag gctatcttgt
    27481 ttattttttc agtttgactt tatccgtaat gatctattat tccttcagcg caatgaccta
    27541 cgatcaatct ttagtcagaa gagcaagtca agatacttcg atagatggtg gattgagttt
    27601 tggtggattt atcattactg ttgttttgct ttttttcgta ttcagtgcca accgattttt
    27661 cttgaataga aggcaaaagg aaataggcat ctatcagctt tttggtatga ataaactgca
    27721 gatatcaggt atatatgtgt tagaaatcat gataattgga ttgttcgcct gtatttctgg
    27781 gatattactc ggtattattt tttccaaact cttttcaatg attttggttc gaatgatgga
    27841 tatggatctt actagtccat tttttatctc cattccatca gtagtagata ctttatttgc
    27901 ttttttcatc atccttttag cagtatctgt atattcaata tggaaaatat ggcgctatcc
    27961 aatgattcga agttttggag aaaaagaaca agcagatagt tcaacaatgc gcttcaggac
    28021 aaggcaccga ttactcgcag tcatcgggtt actactttta acgagcggat attttggagc
    28081 tagtcatttt agggaaatca cttcatggat gatcagttat cattatcttg atacattagt
    28141 tttctttttt ccttttttaa tttttttcgt ttgtgtggtc ggtacttatt tgtttttctg
    28201 ttatacattt cggttatttt tgatcatatt gagtaaatgg aagatttatt actatggtat
    28261 caattttttg ataatgggta acacgcaagt tcatttgctc aaaagttggc gtacaaattc
    28321 tctgattaca gtcattctcg gaatttcttt aatgacaatt ggcggcacgg ctagtatctc
    28381 tactatttta tcctaccagg agaaagtttc ctccccgatg gactatcagt ttgatgttga
    28441 gacagagaaa aaaatcaagc cgattctagc agaggaaggg cagaagataa ctaaagaaat
    28501 caccttgcat tataaagtca ttgggagtat ctatagtcaa aatctcgaag aacctatgtt
    28561 tcaaactgct aatctgatca cggaaacaca atatcaagcc ttcagaaaag ttaatccaga
    28621 tttgccgaac atttctttga aatcacctgg tcatactgtt ttgcttgatc aaatgcaaac
    28681 tctttttcgg aatatttcat tatatggatc tagtattgga ctgccagata atcaagaatt
    28741 gaagattcaa caaatgcttc caagtgtatt aggtgacgaa acaatggtat atctaagtcc
    28801 tgtactgatt gtagatgata ccgtttttga aaaagcaaaa ggtgtggatt atcaagtagt
    28861 caatgtagat gtatcaggtg gaaataatga aaaaatagat gaacgactga gcaatgacct
    28921 gtcgataaag tggctcaatg ctgtttatta ttcttatgat atcgtaaatg gaagtctaca
    28981 aggagaaatt agcacaaaga aacttccgga tgaccaaatg aaggaagcac aatttagcca
    29041 agaaagctct cgtttgaatt tctcttccag ctattcgaaa ttgcgtgcag ttaatcggcg
    29101 aatcggtatc aatatttttg tcacactatt tgtcgggatg atcgtgatta ttgctactgg
    29161 tagtattttg acagtccggc aattagcaga agcagaggaa gaaaaagaaa attatcacct
    29221 attgactaag ctggggattc cacaaagaag gatcagacgg atgatcttcg agcaaaacgc
    29281 acttaccttc tttccaccca tgatattagg gatcactcac gcagtatttg caatcaatgt
    29341 cttcactcag tatataaaaa ctgccgatta ctggttggct tatcttgtga gtggcttatt
    29401 gatccttatc tatcttttgc tttatgtcat tacgagtcag ttatattgtc ggattatcga
    29461 g
//
LOCUS       DACDHJ010000013        29103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDHJ010000013 DACDHJ010000000
VERSION     DACDHJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.013"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IV223_000479"
     CDS             complement(<1..1030)
                     /locus_tag="IV223_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8710787.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IV223_000480"
     CDS             complement(1112..1801)
                     /locus_tag="IV223_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8710788.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV223_000481"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV223_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8710789.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IV223_000482"
     CDS             complement(2328..2831)
                     /locus_tag="IV223_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8710790.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV223_000483"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV223_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8710791.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV223_000484"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV223_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8710792.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV223_000485"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV223_000485"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8710793.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV223_000486"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV223_000486"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8710794.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IV223_000487"
     CDS             complement(6443..7462)
                     /locus_tag="IV223_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8710795.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IV223_000488"
     CDS             complement(7635..8522)
                     /locus_tag="IV223_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8710796.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV223_000489"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV223_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8710797.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV223_000490"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV223_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8710798.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV223_000491"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV223_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8710799.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IV223_000492"
     CDS             9753..10397
                     /locus_tag="IV223_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8710800.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IV223_000493"
     CDS             complement(10453..10956)
                     /locus_tag="IV223_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8710801.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IV223_000494"
     CDS             11553..12518
                     /locus_tag="IV223_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8710802.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IV223_000495"
     CDS             complement(12617..13789)
                     /locus_tag="IV223_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710803.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IV223_000496"
     CDS             complement(13776..14921)
                     /locus_tag="IV223_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8710804.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IV223_000497"
     CDS             complement(14935..15867)
                     /locus_tag="IV223_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710805.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IV223_000498"
     CDS             15984..17063
                     /locus_tag="IV223_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8710806.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IV223_000499"
     CDS             17060..17689
                     /locus_tag="IV223_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8710807.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IV223_000500"
     CDS             complement(17799..18026)
                     /locus_tag="IV223_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8710808.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IV223_000501"
     CDS             complement(18129..20717)
                     /locus_tag="IV223_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8710809.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IV223_000502"
     CDS             complement(20737..21396)
                     /locus_tag="IV223_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8710810.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IV223_000503"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IV223_000503"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8710811.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IV223_000504"
     CDS             complement(22169..23035)
                     /locus_tag="IV223_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8710812.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IV223_000505"
     CDS             23144..23785
                     /locus_tag="IV223_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8710813.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IV223_000506"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IV223_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8710814.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IV223_000507"
     CDS             complement(25226..26125)
                     /locus_tag="IV223_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8710815.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IV223_000508"
     CDS             complement(26127..26924)
                     /locus_tag="IV223_000508"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8710816.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IV223_000509"
     CDS             complement(26902..27606)
                     /locus_tag="IV223_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8710817.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IV223_000510"
     CDS             27737..28312
                     /locus_tag="IV223_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8710818.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="IV223_000511"
     CDS             28430..>29103
                     /locus_tag="IV223_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8710819.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8561 a         6169 c         5232 g         9141 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DACDHJ010000014        28084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDHJ010000014 DACDHJ010000000
VERSION     DACDHJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.014"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..551)
                     /locus_tag="IV223_000512"
     CDS             complement(21..551)
                     /locus_tag="IV223_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8710820.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(555..1415)
                     /gene="rlmB"
                     /locus_tag="IV223_000513"
     CDS             complement(555..1415)
                     /gene="rlmB"
                     /locus_tag="IV223_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8710821.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1408..1803)
                     /locus_tag="IV223_000514"
     CDS             complement(1408..1803)
                     /locus_tag="IV223_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8710822.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(1800..3212)
                     /locus_tag="IV223_000515"
     CDS             complement(1800..3212)
                     /locus_tag="IV223_000515"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8710823.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3209..3745)
                     /gene="cysE"
                     /locus_tag="IV223_000516"
     CDS             complement(3209..3745)
                     /gene="cysE"
                     /locus_tag="IV223_000516"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8710824.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4067..5527)
                     /locus_tag="IV223_000517"
     CDS             complement(4067..5527)
                     /locus_tag="IV223_000517"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8710825.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(5697..6854)
                     /locus_tag="IV223_000518"
     CDS             complement(5697..6854)
                     /locus_tag="IV223_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8710826.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(6888..8258)
                     /gene="radA"
                     /locus_tag="IV223_000519"
     CDS             complement(6888..8258)
                     /gene="radA"
                     /locus_tag="IV223_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8710827.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8321..8848)
                     /locus_tag="IV223_000520"
     CDS             complement(8321..8848)
                     /locus_tag="IV223_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8710828.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9078..9884
                     /gene="proB"
                     /locus_tag="IV223_000521"
     CDS             9078..9884
                     /gene="proB"
                     /locus_tag="IV223_000521"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8710829.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            9881..11128
                     /locus_tag="IV223_000522"
     CDS             9881..11128
                     /locus_tag="IV223_000522"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8710830.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11204..11548
                     /locus_tag="IV223_000523"
     CDS             11204..11548
                     /locus_tag="IV223_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710831.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(11665..11991)
                     /locus_tag="IV223_000524"
     CDS             complement(11665..11991)
                     /locus_tag="IV223_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710832.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(12606..13808)
                     /locus_tag="IV223_000525"
     CDS             complement(12606..13808)
                     /locus_tag="IV223_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8710833.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            13925..14440
                     /locus_tag="IV223_000526"
     CDS             13925..14440
                     /locus_tag="IV223_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8710834.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(14639..15085)
                     /locus_tag="IV223_000527"
     CDS             complement(14639..15085)
                     /locus_tag="IV223_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8710835.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15132..16610)
                     /gene="msr"
                     /locus_tag="IV223_000528"
     CDS             complement(15132..16610)
                     /gene="msr"
                     /locus_tag="IV223_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8710836.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(16964..17593)
                     /locus_tag="IV223_000529"
     CDS             complement(16964..17593)
                     /locus_tag="IV223_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8710837.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(17577..18041)
                     /locus_tag="IV223_000530"
     CDS             complement(17577..18041)
                     /locus_tag="IV223_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8710838.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18528..19796)
                     /locus_tag="IV223_000531"
     CDS             complement(18528..19796)
                     /locus_tag="IV223_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8710839.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20027..20764)
                     /locus_tag="IV223_000532"
     CDS             complement(20027..20764)
                     /locus_tag="IV223_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8710840.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(20831..21400)
                     /locus_tag="IV223_000533"
     CDS             complement(20831..21400)
                     /locus_tag="IV223_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8710841.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            21528..22400
                     /locus_tag="IV223_000534"
     CDS             21528..22400
                     /locus_tag="IV223_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8710842.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            22742..23614
                     /locus_tag="IV223_000535"
     CDS             22742..23614
                     /locus_tag="IV223_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8710843.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            23611..25569
                     /locus_tag="IV223_000536"
     CDS             23611..25569
                     /locus_tag="IV223_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8710844.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(25901..26752)
                     /locus_tag="IV223_000537"
     CDS             complement(25901..26752)
                     /locus_tag="IV223_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8710845.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            26875..27342
                     /locus_tag="IV223_000538"
     CDS             26875..27342
                     /locus_tag="IV223_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8710846.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(27605..>28084)
                     /locus_tag="IV223_000539"
     CDS             complement(27605..>28084)
                     /locus_tag="IV223_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8710847.1"
                     /translation="AKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIGN
                     GTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIAL
                     AESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP"
BASE COUNT         8001 a         5965 c         5153 g         8965 t
ORIGIN      
        1 gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa tttttgggac
       61 aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat aaaatgcata
      121 tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata taattcataa
      181 gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa atcgcttgtc
      241 gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc gatataactg
      301 tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt ataattttgt
      361 tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc ttcatatttc
      421 gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt gtcctgcttt
      481 ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac aatcaacagc
      541 tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat aagcaaacca
      601 gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt cagcaattca
      661 tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat gactaaagca
      721 attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt gccaaatacc
      781 caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac gcgtgcaaca
      841 gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat tccaactgct
      901 cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg taaaatcgac
      961 ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc ttcttttttc
     1021 tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt aatcgcaaga
     1081 acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt aggaacccat
     1141 ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt ttctcctcgt
     1201 gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc tactgcggca
     1261 tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga aactgactga
     1321 tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc aaatccacgt
     1381 ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt tctgaataca
     1441 ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat agcccatgag
     1501 cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg cgctcgtatg
     1561 gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca aaagatgttc
     1621 ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat tcgttgccgc
     1681 acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac gaacatagat
     1741 ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg tgtaatctct
     1801 catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat ccctttttct
     1861 ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct tgcttgattt
     1921 cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt aaataaaata
     1981 ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac agcggcagaa
     2041 actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac cactgtgatc
     2101 ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc taaattttct
     2161 aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc ttggcggaat
     2221 ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc tttcatcgtc
     2281 gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa acgcaagact
     2341 tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag agatttactc
     2401 attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta attagcgaat
     2461 tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg aaattctaaa
     2521 tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc cataacggaa
     2581 cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga aatctcgcca
     2641 ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg ctgttcttcc
     2701 cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact taatttccca
     2761 taattttcaa atttacgggt acggtaatac acatcacctg aagattcata agcatagcct
     2821 ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat gactcttgga
     2881 tgcagtgtag caggttcgac attcaacgct ttcgtatctt cttcaaatgc tcggatgaaa
     2941 cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat gattttatcg
     3001 tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc aaaatagcgt
     3061 cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata attatacaca
     3121 gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa ttcttctttt
     3181 tttctggtca atgtattgta gatttttatc attctgatct cctcactttt tctcctttga
     3241 ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca tctttcaaca
     3301 caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct aatatctgcg
     3361 cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc gtttcttttc
     3421 ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt tctgcggttt
     3481 cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc gttgctcctg
     3541 gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc gccaataaaa
     3601 agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca tgaaaaccag
     3661 ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc tttgctgcat
     3721 caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat tttatgtagg
     3781 aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta tcaaaagacg
     3841 ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag ctaacgcctg
     3901 ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt ctgtgtttcg
     3961 ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca ccacccggta
     4021 tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag ttcagtactt
     4081 gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca ggcaattctg
     4141 gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt tttcctttta
     4201 cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca tctactactt
     4261 ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt tcaccagcaa
     4321 gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc gtagacagat
     4381 caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc ttcttcaccc
     4441 attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt agataaggca
     4501 gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg tttacccatt
     4561 gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga tcgaacattt
     4621 tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag cccaataatg
     4681 caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt tcgataaatt
     4741 gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg atgatcaatg
     4801 tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc agttgttttg
     4861 gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc aaatgatcat
     4921 cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttggatg acaaagtcgc
     4981 caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta aatgcgtatt
     5041 ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct tctttttctg
     5101 cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt tctcctcttg
     5161 cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat ttgtatgctc
     5221 gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt tcagattgac
     5281 ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt cccaaccaag
     5341 caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg tcagtatcct
     5401 caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg aataatgcag
     5461 ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt acgcgtactt
     5521 tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa attccgtcag
     5581 ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta ccttttctaa
     5641 ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt tcttttttat
     5701 tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg tcctgcatgt
     5761 gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt tacaacgact
     5821 tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt gccgtcatca
     5881 aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac attcatcgtt
     5941 tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt aagaacaggt
     6001 acattttgga attcagagac tttattcaag ttatagtcat tggttacaat gacaccatct
     6061 aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc aaagtctcca
     6121 tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat atctaatccg
     6181 cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg tagttcgtaa
     6241 agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt aatatcataa
     6301 atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa attttctcct
     6361 gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc gatttttttg
     6421 cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat tctcaatccc
     6481 agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa tggaatttcc
     6541 atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat cgcaccgatc
     6601 gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac ttgtttgata
     6661 atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag tgcgcctaac
     6721 aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat tgcctgccaa
     6781 gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag caaagtaatg
     6841 acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta gcggaacaca
     6901 cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt tggcgcttcc
     6961 cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt ttggacttct
     7021 ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc aatttcaccg
     7081 atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat actgacagcg
     7141 atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt taagtaagca
     7201 tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat cagagatacc
     7261 cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt aggtgtgact
     7321 aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac gatagcagag
     7381 ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc gacttcttcc
     7441 aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa tcggtttttt
     7501 actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa caccgtatcc
     7561 accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac atgaccgacg
     7621 ataaaaatgg ctatcccatt tgttttagcg gttttgagta gttctgcagt ggtttctctt
     7681 acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt ttggatggaa
     7741 tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc gttcatatct
     7801 gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc gcgtaatttt
     7861 atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat ggctgctaat
     7921 tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc accaatcaag
     7981 accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc taattttgtt
     8041 ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt tgctttttct
     8101 cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc ttcgaccatc
     8161 gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac atacccgcaa
     8221 ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc ttttatccta
     8281 atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg gaagaaccaa
     8341 atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac ggcaaaaaga
     8401 tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca aagttggtga
     8461 attggaacat gatatggcct tcatttgcct cattattgta ataatcactg tcaatcacac
     8521 ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg ttagcaagct
     8581 gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg gttggtttca
     8641 attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt tccaacccct
     8701 ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct tcaaaatcat
     8761 agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct ttatatttag
     8821 aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac tactcctatt
     8881 ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa ttctgctatc
     8941 ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac aaaaaaacta
     9001 ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa tgagtggata
     9061 tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt attgtcaaag
     9121 taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca atcgatcagt
     9181 tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta gtctcatcag
     9241 gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact ttgattcctg
     9301 aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat aatcaaagat
     9361 tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg gtagattatc
     9421 ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg gacacgatcc
     9481 ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg acaaaatttg
     9541 gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac cttttgatcg
     9601 tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc gaggctcaga
     9661 tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt ggtgctggta
     9721 gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg atatttgaag
     9781 caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt gatattcttg
     9841 caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact tgatgcaatt
     9901 aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa accgcaaaaa
     9961 tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga tcattgaagc
    10021 aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga tgcaagaccg
    10081 cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa aaattgcttc
    10141 gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg gattgagaat
    10201 cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc gtccaaatgt
    10261 cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc ttcgcggtgg
    10321 caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag tactggtcag
    10381 tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg aaactgcagg
    10441 acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg gagcaaaatt
    10501 aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta caggaaataa
    10561 tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca tcgtcaatgc
    10621 aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc attctgagat
    10681 cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg tctctttacg
    10741 tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag acacagattg
    10801 ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt tggatgaagc
    10861 aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga cagatagtta
    10921 tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg tcaatgcctc
    10981 cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta tctctactca
    11041 aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa aatatatcat
    11101 ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc ccgccttttg
    11161 ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg aaattttaat
    11221 gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag tcggtatttt
    11281 atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa aatggtcgca
    11341 gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta gttacatggt
    11401 tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt caatcgctta
    11461 tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc agctgaataa
    11521 aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag agtgccttga
    11581 agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg tcacagcctc
    11641 tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt cctcttcgtt
    11701 aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct catccatgga
    11761 caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct gttcccgata
    11821 cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac cttccgcatt
    11881 gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg aaaattcaat
    11941 gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca tctattatcc
    12001 ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc agtttcatta
    12061 ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt ccgaataaat
    12121 ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca tgagaaactg
    12181 ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac ctcttggtaa
    12241 acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat atgagaagct
    12301 atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca acctctgtta
    12361 aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
    12421 tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca acctcctgat
    12481 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgaga
    12541 agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc acaacctctg
    12601 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
    12661 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
    12721 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
    12781 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
    12841 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
    12901 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
    12961 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
    13021 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
    13081 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
    13141 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
    13201 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
    13261 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
    13321 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
    13381 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
    13441 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
    13501 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc
    13561 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
    13621 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
    13681 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
    13741 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
    13801 atttccatcc ctttctctct attagcatca tacatgattt attttacatg tcaatatgca
    13861 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
    13921 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
    13981 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
    14041 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
    14101 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
    14161 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
    14221 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
    14281 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
    14341 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
    14401 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
    14461 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
    14521 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
    14581 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
    14641 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
    14701 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
    14761 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
    14821 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
    14881 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
    14941 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
    15001 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
    15061 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
    15121 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
    15181 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
    15241 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
    15301 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
    15361 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
    15421 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
    15481 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
    15541 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
    15601 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
    15661 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
    15721 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
    15781 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
    15841 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cagtctgatt
    15901 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
    15961 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
    16021 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
    16081 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
    16141 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
    16201 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
    16261 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
    16321 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
    16381 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
    16441 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
    16501 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
    16561 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
    16621 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
    16681 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
    16741 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
    16801 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
    16861 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
    16921 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacatagtga
    16981 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
    17041 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
    17101 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
    17161 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
    17221 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
    17281 ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc atatcgccaa
    17341 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
    17401 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt ttcatattga
    17461 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
    17521 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
    17581 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
    17641 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
    17701 agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt ctgggctttt
    17761 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
    17821 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
    17881 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
    17941 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
    18001 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
    18061 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
    18121 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
    18181 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
    18241 tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc aaaaatttcc
    18301 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
    18361 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
    18421 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
    18481 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
    18541 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
    18601 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
    18661 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
    18721 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
    18781 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
    18841 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
    18901 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
    18961 gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata acgacctgtt
    19021 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
    19081 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
    19141 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
    19201 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
    19261 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
    19321 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
    19381 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
    19441 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
    19501 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
    19561 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
    19621 tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc tgtttctgaa
    19681 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
    19741 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
    19801 tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca
    19861 agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct ttttctgcat
    19921 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
    19981 ataactattt aacaagtaat tctctctgga tattatccgt tatggattat acttcctgtg
    20041 tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct atcgcaaatg
    20101 ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct tcatgaagtt
    20161 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
    20221 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
    20281 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
    20341 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
    20401 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
    20461 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
    20521 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
    20581 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
    20641 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
    20701 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
    20761 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
    20821 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
    20881 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
    20941 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
    21001 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
    21061 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
    21121 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
    21181 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
    21241 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
    21301 gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
    21361 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
    21421 ttactttata acggacggct tatgatgtct aatacttata aaacatactt aactattcct
    21481 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
    21541 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
    21601 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
    21661 tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt cgatatcttt
    21721 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
    21781 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
    21841 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
    21901 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
    21961 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
    22021 ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc catccgcagg
    22081 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
    22141 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
    22201 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
    22261 atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa gcttcactga
    22321 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
    22381 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
    22441 ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt gttccttcct
    22501 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
    22561 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
    22621 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
    22681 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
    22741 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
    22801 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
    22861 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
    22921 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
    22981 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
    23041 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
    23101 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
    23161 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
    23221 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
    23281 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
    23341 gaattattca agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca
    23401 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
    23461 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
    23521 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
    23581 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
    23641 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
    23701 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
    23761 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
    23821 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
    23881 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
    23941 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
    24001 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
    24061 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
    24121 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
    24181 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
    24241 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
    24301 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
    24361 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
    24421 aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg tgtaacagaa
    24481 ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
    24541 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
    24601 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
    24661 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
    24721 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
    24781 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
    24841 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
    24901 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
    24961 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
    25021 gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa caccgtggtt
    25081 aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat cgcaaatggg
    25141 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
    25201 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
    25261 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
    25321 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtaaaa
    25381 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
    25441 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
    25501 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
    25561 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
    25621 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
    25681 gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac tcttcttatt
    25741 acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa accagtttat
    25801 cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttca
    25861 tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga tccaatattt
    25921 cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct aaatcatcag
    25981 aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac atgtcagctg
    26041 cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca cttgcttcaa
    26101 taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct tcatagtcag
    26161 ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt acttttgctg
    26221 cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct gcagcataaa
    26281 cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct acataccaat
    26341 tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca gtgaattggt
    26401 gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta taagcgatat
    26461 aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct tgacgatttc
    26521 ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct ttcattgagt
    26581 ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt gcttctcctt
    26641 tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt tttaagaatt
    26701 ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc atatttgcca
    26761 ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt acacctttat
    26821 cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca actgatgaag
    26881 tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat gtttcaagat
    26941 acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc agcaaatgta
    27001 cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg
    27061 cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta tcgcagcatc
    27121 gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt
    27181 gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa
    27241 gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt ggaaaaagaa
    27301 aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct actgtgcctg
    27361 catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga caaaagcctt
    27421 gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga
    27481 taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa aatatggaaa
    27541 gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca cgacctctat
    27601 attatcatgg tttattgact accggtatcc agatttctcc catatatttc ccgttggtaa
    27661 ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct acttctctta
    27721 aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc gattctgcta
    27781 aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta gtgtttggaa
    27841 catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag ggttcgttga
    27901 ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt ccattaccga
    27961 taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc gtattgctac
    28021 tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg atcctatact
    28081 ttgc
//
LOCUS       DACDHJ010000015        27729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDHJ010000015 DACDHJ010000000
VERSION     DACDHJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.015"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            53..517
                     /locus_tag="IV223_000540"
     CDS             53..517
                     /locus_tag="IV223_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710848.1"
                     /translation="MELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQI
                     DDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHR
                     FIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            576..1016
                     /locus_tag="IV223_000541"
     CDS             576..1016
                     /locus_tag="IV223_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710849.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1028..1696
                     /locus_tag="IV223_000542"
     CDS             1028..1696
                     /locus_tag="IV223_000542"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8710850.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1760..2383
                     /locus_tag="IV223_000543"
     CDS             1760..2383
                     /locus_tag="IV223_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8710851.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2439..2825
                     /locus_tag="IV223_000544"
     CDS             2439..2825
                     /locus_tag="IV223_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8710852.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3038..4429
                     /locus_tag="IV223_000545"
     CDS             3038..4429
                     /locus_tag="IV223_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8710853.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5040..5408
                     /locus_tag="IV223_000546"
     CDS             5040..5408
                     /locus_tag="IV223_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8710854.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5588..6529
                     /locus_tag="IV223_000547"
     CDS             5588..6529
                     /locus_tag="IV223_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8710855.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6598..7329
                     /locus_tag="IV223_000548"
     CDS             6598..7329
                     /locus_tag="IV223_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8710856.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7473..9653
                     /locus_tag="IV223_000549"
     CDS             7473..9653
                     /locus_tag="IV223_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8710857.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTELIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9673..10155
                     /locus_tag="IV223_000550"
     CDS             9673..10155
                     /locus_tag="IV223_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8710858.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10171..10932
                     /locus_tag="IV223_000551"
     CDS             10171..10932
                     /locus_tag="IV223_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8710859.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11063..12397
                     /locus_tag="IV223_000552"
     CDS             11063..12397
                     /locus_tag="IV223_000552"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8710860.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12704..13189
                     /locus_tag="IV223_000553"
     CDS             12704..13189
                     /locus_tag="IV223_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8710861.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13182..13865
                     /locus_tag="IV223_000554"
     CDS             13182..13865
                     /locus_tag="IV223_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8710862.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13991..16636
                     /gene="polA"
                     /locus_tag="IV223_000555"
     CDS             13991..16636
                     /gene="polA"
                     /locus_tag="IV223_000555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8710863.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16681..17517
                     /gene="mutM"
                     /locus_tag="IV223_000556"
     CDS             16681..17517
                     /gene="mutM"
                     /locus_tag="IV223_000556"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8710864.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17481..18110
                     /locus_tag="IV223_000557"
     CDS             17481..18110
                     /locus_tag="IV223_000557"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8710865.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18162..18494
                     /locus_tag="IV223_000558"
     CDS             18162..18494
                     /locus_tag="IV223_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8710866.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18806..19297
                     /gene="nrdR"
                     /locus_tag="IV223_000559"
     CDS             18806..19297
                     /gene="nrdR"
                     /locus_tag="IV223_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8710867.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19320..20693
                     /locus_tag="IV223_000560"
     CDS             19320..20693
                     /locus_tag="IV223_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8710868.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20693..21619
                     /gene="dnaI"
                     /locus_tag="IV223_000561"
     CDS             20693..21619
                     /gene="dnaI"
                     /locus_tag="IV223_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8710869.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21748..22188
                     /locus_tag="IV223_000562"
     CDS             21748..22188
                     /locus_tag="IV223_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8710870.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22332..23033
                     /locus_tag="IV223_000563"
     CDS             22332..23033
                     /locus_tag="IV223_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8710871.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23030..24151
                     /locus_tag="IV223_000564"
     CDS             23030..24151
                     /locus_tag="IV223_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8710872.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24166..25398
                     /gene="pepT"
                     /locus_tag="IV223_000565"
     CDS             24166..25398
                     /gene="pepT"
                     /locus_tag="IV223_000565"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8710873.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25530..26438
                     /locus_tag="IV223_000566"
     CDS             25530..26438
                     /locus_tag="IV223_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8710874.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26473..26745
                     /locus_tag="IV223_000567"
     CDS             26473..26745
                     /locus_tag="IV223_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8710875.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26756..>27729
                     /locus_tag="IV223_000568"
     CDS             26756..>27729
                     /locus_tag="IV223_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8710876.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYV"
BASE COUNT         9308 a         4810 c         6045 g         7566 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtatcgagct
       61 ttctcttgaa tcttgggatc gagcagtggt tgaactgttt ccagaaggtt taatgatcga
      121 tacaaacgat gggaagatcc atctgactgc agcagaactg caaaaacaaa tgcgttatca
      181 aatagatgac gaaaattctt tttctatttt tagacgtcag caaaagattc taaaaagctt
      241 tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg acacagctgt tgaaaaaata
      301 tccaggtctg cttcacacgt ctattcaatt tcctcaattg attacgatga tccatcgttt
      361 tatacgtatg caacagctac catctaagaa gctgtttctt ccattgacgg acacatttcg
      421 agtagtcaac agagaagata aaaagaaagt gattgtgatt gactttacca gaaatcggaa
      481 tgtccttcac caagttgcaa tggaaaaggc caattaaggc tttttctttt tggatttttt
      541 tgtcataata aatattagat gatggaggag ataatatgat gcaaacatgg ttagaaaggc
      601 taatacgatt tcagcagaac tatcacaaac ttatgcaggg aagatatgcg aaatttgatc
      661 agttgaatcg cggtctattg atcgctgcgc ttgtctttag tttgctaagt cgttggttgc
      721 cctatagaat cggacaattg ttgttcatcg tctttttcgg atggatgata tttcgtttcc
      781 tatcaaaaaa aatctacccc agactaaatg agaatcaacg ttatttgaaa tatcttgata
      841 gagtcaaaga aaaatatcat cagataaaaa acaaaaagca agtcaaaatg gatcgaaaaa
      901 gttatacttt ttttgagtgc ccgaattgtc atcaaaagca gcgtgcacct aaaggaaaag
      961 gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt cgaaacgaat gtttaaggga
     1021 gaaaagaatg aaattagtat tattacgtca tggcgaaagc gaagcaaatt ttgaaaatta
     1081 ctggacagga tggctagatg tcgccttaac agaaaaagga aaagaacaag cagcagttgc
     1141 cggtcaaaaa atgcttgaag ccggacttca attcgatgcc gtttatacct ctgttttgaa
     1201 acgtgcgatt cttaccggac aagtcgcact agaagaaatg ggtcagttat ggcttcctat
     1261 aataaaaaca tggcgtctaa atgaacggca ttatggggat cttgtgggta aaaacaaaga
     1321 taagatgaaa gaaatatacg gaaaagatca agtaaaaaca tggcgcagag gatatacgga
     1381 agttccgccg ttaaccgcag aaaatagatt tgaccgcaga tatgatcact tggatccgca
     1441 tttgatccca tacggagaaa gtctggaaat gacagtgaat cggatcattc ccttatggca
     1501 agatcatcta gcaccaaaac taaaagatcg gcaagattta ctagttgtgg gtcatggtaa
     1561 tagcatccga gcactgacaa aatatttaga agatgttcct gaagatcaaa tggatacgat
     1621 cgatatccca aatgcacaac caatccagta tacattagca gatgatttga caattctgga
     1681 caaaaaaata ctttaaagaa cacctttatc taaaagtgag agatagaaaa aagaagcaaa
     1741 ggaatggaac aaaaggaaca tgaaaaatta tatatgggac tttgatggta cattgtttga
     1801 tacgtatccc gcaatggtag atggtgcatg gcaagcacta aaagattttg gtatcagtat
     1861 ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct actagttatt tgatcaatga
     1921 atcaaacttg aatgccagag agtttaatga attatttcac cgatatgaaa aagaaagtac
     1981 ggaagtttcc cgtccatttc cagaaacgaa acaagtactg gaaatgctaa aagacaacgg
     2041 cggtcgtcat ttcatcctga cccaccgttt gacagaatca acatggggcc tgttaaaaga
     2101 acatcgattg gcacatctga ttgaggaagt agtagggatc gatcaggact ttcctcgtaa
     2161 acctgatcca gcatctttaa attatttgat cgatactttt catttagaaa gaacagacac
     2221 gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg aaaaatgcag gagtcgctac
     2281 ttgtctttac gacatcgatc attttctggg tgagatcccg gcagattatg tggtaggaaa
     2341 tctgaatgaa atccttacct tgcagagcat cattaaaaag tagtatcccc tttcaaagga
     2401 gatatgttat aattttcata aaggagtgat aaaattatat ggaaatcaga agacaaaaag
     2461 aagttgtaga agcatttcat tatgatatga gattgccgaa ccaagaagta gaaacagatt
     2521 tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa ctatccgaaa gaacatagca
     2581 tcgtaggagc acgcttagag ttccgtttag tgtttgatac atatgtttta tctggaagtg
     2641 tgagtcagat caatcatatc ctcaatcgga aaattgaagg tcaaaatgat gtcacacaag
     2701 aagaagtgga cgagctagta gcaccgttat tcgatatcgt acgccgaatg tcttatgagg
     2761 tgacagagat cgccttagat cagcctgggg taaaattgaa ttttcaatca gaaaaacaag
     2821 aatagacagt aaaaacatac ctgcgataga ttaaagctat cacaggtatg tttttttatt
     2881 actaattgta agcaaaaaga atgtcatttc gataaaaatc gtctaaaata taaaagtcac
     2941 tgaaaaaaat tttttcagga ggatggttga agaatcattc tcgggaacct ttacttcctt
     3001 cgatggactt gtcattaata atatgaggag gagaaaaatg acaacactca tcacagtttt
     3061 agtggttcta gtttttgcgg cattattata tgtgttctat cgtatgcaaa agcgccatac
     3121 aaaattttca acacgcgtct ttacagcatt aggagttggt atcgtctttg gcggtctgct
     3181 tcaattgatc tttggtgtag acgacaaagt cgttactcaa tcaatggaat ggatcgggat
     3241 tgtaggtcaa ggatatgtat cattcttgca aatgctagtt atgcctttag tgtttatttc
     3301 gatcattggt gcattcacaa aaatgaaagt ttctgaaaag ctagggaaaa ttagtgcgac
     3361 tgtattgact tctttacttg gaacaacagc tattgcagca ttgatcggaa ttggttcaac
     3421 aatgctgttc ggactccaag gagctaaatt cacacaagga gctgcagaaa cagcacgaat
     3481 ctctgaactg gcaacacgtc aggaaacagt tgaaaactta tcgattccac agcagatttt
     3541 atcctttatc cctaaaaacg tttttgctga tttagcaggt agtcgtgcaa caagtacgat
     3601 tggagtcgtt attttcgcgg catttgtcgg aatcgcttat ctaggcgtga aaagaaaagc
     3661 gccaaaagaa gcagcattct tttcagattt agtcgacagt ctgtataaaa tcattatgcg
     3721 gatcgtgacg cttgttttgc gtttgacacc ttatggagta tttgctttga tgacaaacgt
     3781 tttggcaaca agtgattttg cagctatcgt caatttaggt aaatttgtcg ttgcttctta
     3841 cgtagcattg cttgctgttt tgattgtcca ctcactgatt ttgattggtg caaaagtcaa
     3901 tccaatcaac tatttcaaaa aagcctttcc ggtattgagc tttgcgttta cttcacgttc
     3961 aagtgctgga gctttgccat tgaatatcga gactcagaca aaagctttag gagtagatga
     4021 tgcaacagct aatttctcag caagttttgg tttgtctatt ggacaaaacg gctgtgctgg
     4081 cgtttatcca gctatgttgg caacgatcgt cgcaccagca gctggagtga atgtctttag
     4141 tttggaattt attttgatgc ttgtggcaat tgtaacaatc agttcttttg gtgtagctgg
     4201 agtcggtggg ggcgcaacat ttgcttcact gatcgtttta ggggcaatga acttgccagt
     4261 agcaatcgtt gggttagtta tctctgttga accattaatc gatatggcac gtacagcagt
     4321 aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt gcacgcatca aagatttaga
     4381 tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg aatgcatagt caaaaagagg
     4441 ttgtgataga agtggtcagc tttaagaaat aagaagaagt ttcctaaaaa tagtttctca
     4501 aatttttgtg aaatttcggc ttatttccga gaagctactt ctgaaacacc gtgaataaga
     4561 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     4621 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     4681 aagaggtcgt gagcctgccg tttcactctg agtcacaaag agcaatttca aaaaatagct
     4741 tctcatgttt gttgaatatt gtgtcttgtg accgaggagt tgtcaggaga acaccgttta
     4801 ccagaggttg tgacttgttt ttagtcacga cctctttttt tatttattcc atataaatac
     4861 acattattta ttccgaaaag tgaaagcgca aataacaaaa acaaccaact tatttattat
     4921 taaagaaaag cgtatttaag taggacgatt ctgttgagag ctttcgtaga ttttcccatt
     4981 ttcctatggt aaactgacaa agttgagaat gttagaacag atcgtttagg aggaaacaga
     5041 tgtttacgaa tgaaataaaa atcatgcttt atgtggacga tgtagaaaaa aatgcaacgt
     5101 tttggagagc agttggcttt gcggaaaaag atcgtcaaga aatggatggg acgcttataa
     5161 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
     5221 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
     5281 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
     5341 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
     5401 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
     5461 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
     5521 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
     5581 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
     5641 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
     5701 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
     5761 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
     5821 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
     5881 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
     5941 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
     6001 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     6061 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     6121 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     6181 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     6241 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     6301 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     6361 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     6421 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     6481 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     6541 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     6601 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     6661 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     6721 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     6781 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     6841 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     6901 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     6961 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     7021 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     7081 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     7141 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     7201 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     7261 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     7321 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     7381 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     7441 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     7501 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     7561 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     7621 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     7681 tagaaaaacg taaaactgaa ttgattcggc taatcgatga acaagggaaa ctgactcccg
     7741 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     7801 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     7861 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     7921 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     7981 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     8041 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     8101 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     8161 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     8221 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     8281 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     8341 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     8401 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     8461 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     8521 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     8581 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     8641 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     8701 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     8761 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     8821 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     8881 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     8941 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     9001 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     9061 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     9121 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     9181 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     9241 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     9301 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     9361 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     9421 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     9481 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     9541 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     9601 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     9661 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     9721 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     9781 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     9841 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     9901 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     9961 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
    10021 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
    10081 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
    10141 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
    10201 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
    10261 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
    10321 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
    10381 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
    10441 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
    10501 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
    10561 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
    10621 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
    10681 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
    10741 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
    10801 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
    10861 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
    10921 tctcgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
    10981 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
    11041 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
    11101 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
    11161 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
    11221 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
    11281 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
    11341 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
    11401 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
    11461 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
    11521 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
    11581 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
    11641 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
    11701 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
    11761 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
    11821 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
    11881 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
    11941 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
    12001 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
    12061 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
    12121 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
    12181 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
    12241 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
    12301 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
    12361 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
    12421 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
    12481 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
    12541 gaagctgttt tttcttggct gttcttatta tttgatgcag gacagctttt tcacgacctc
    12601 tgtaaactat aggaatctgt taaatcaagg gagcagaact tgccttttac gctacttttg
    12661 gtacaataat gtatgattaa aaataaactg gagggtttct atcttgaaca taggtaagcc
    12721 gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc gactcgttat ccttaacaga
    12781 atctattgaa gataaccagc ttttgctgta tttgggattg acgaatgatg caaatccttt
    12841 gtatatccaa catgactttg cacaaaaaac agaatatcaa cagccaatcg ttccatcagt
    12901 gatgctgatg gggatcatca caagtgcaat ttcaaagcat cttcctggac caggatcaca
    12961 tgttgtcaat ttttctgtca attttattga accggttttc cattatgaaa cactgacttt
    13021 tcagtttgag gtcataaaag tagataagat gaaggatgtg gtgacgattt ctgtagaagc
    13081 ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt atggtccaac cgccacaagt
    13141 tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg aatgaataat ccaagtacat
    13201 caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg atttttaggt atgggacttg
    13261 cccttagtgc agtcacttct tacttggctc tttgggtatt tcctttgcaa ttagcaactt
    13321 ttatcaataa ttttccacta ggctttgtgg gtctgtggat cgttgaactg attttagtaa
    13381 tggttctagg cgtaaaagca caaaagaatc cgagcttagc aatcggtgga tttattgcct
    13441 actctctttt gaatggcata acattagcgg ttactcttgc atactacgat attggaacag
    13501 taacgcaagc tttcttgaca gctactttga tgttcatggg gatgtctgtt gtcggagtaa
    13561 caacaaaaag agatcttagc ggggttggaa gagcaggaca ggcagcttta atcggagtga
    13621 tcattgcact agtcttgaac atcttcattt tgagaagtag cgtagttgag ctgttcatct
    13681 ccctactgtt agtcgtcatc tttgcaggga ttacagctta tgataaccaa agcatccgta
    13741 atatctatgt ccagacaaac ggaacagcac atagtggaat tgctgtattc atggcattga
    13801 agctgtattt ggactttatc aacttgttct tggcgtttct gagaatcttt ggacggaaca
    13861 actaaaaaag aaaaaaattc atcagttggg gctataccga attatttggt atggtcccgc
    13921 tttttctttt cgcattccgc aggattcttt gttagaatga ataagaactt tacagtaagg
    13981 agttaagaag atgacaaaaa ataaattatt actcgttgat gggaacagtg tggcttttcg
    14041 cgcattcttt gcgcttcaca attctcttga acgtttcaaa aatagaaacg gtcttcatac
    14101 aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt atgcaaaaag agcaaccgac
    14161 tcatgttctg gttgcgttcg atgcaggaaa aacgactttt cggacagaaa tgtatgcgga
    14221 gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa gaacaaatgc catatatacg
    14281 tgatttgttg acaggactag gggtacagta ttacgaattg cccaattatg aagcagatga
    14341 tattatcggt actttggcag aaaaagtaga caaagatcaa tttgatgtcg tcgtgctttc
    14401 aggtgatcgg gatttgactc aattagctag taaagaagta aaagtggata tcactgtaaa
    14461 aggtgtcagc gatatcgaaa gctatacacc agaacatgta gcggaaaaat atgatgggtt
    14521 gacgccaaaa cagattattg acatgaaggg actagccgga gatgcctctg acaatatccc
    14581 cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta ttgaaacaat atggctcagt
    14641 cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc aaaatgaaag aaaatctgat
    14701 aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg accatcaata cttcagcgcc
    14761 tgtggatgtc tctatcgact cattgaagta tgaagggaaa aatctcgata aactcgttcc
    14821 gttttataaa gaaatggatt tcaatcagtt cctttctaaa ttgaatatcg tagaagaaga
    14881 agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa ttcaaagagg aaatgtttac
    14941 tacggatatg gcgttgtacg tggaaatgat gggcgacaat tatcatacag aggaaatcgt
    15001 cggagtagcg tgggggacag aaaagaaaat ttatgtcacg aacgatctat ctgtattcga
    15061 aaaccgagcg ttccattctt ggatcactga tccgactcgg ctgaaaaaag tatatgatgc
    15121 gaaacgaact tatgttgctt taaaccgcta tactggaaaa acgaaaggaa tcgattttga
    15181 tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa agcacagata tcgaaggaat
    15241 agctgctcat tacggataca acgatatcca atcagatgaa gctgtatacg gcaaaggggc
    15301 gaaaaaaggt ttaccagaag aagaggaact cttttttgct catttagcgc gaaaagtagc
    15361 tgcaatcaat gcgttgacac caaaattgag ccaagaactg gcagaaaaaa accaagaaga
    15421 tcttttccgg aaaatggaac tgccattgtc tatcattttg gcagagatgg aaataagcgg
    15481 gatcaaagta gatgcgactc gcttacaaga aatgaagggc gagttctctg ctcgcttaag
    15541 agagatcgaa caaaaaatct acgaagaagc cggcgaagaa ttcaatttga attcaccaaa
    15601 gcaattagga gtgatcttat ttgaaaaaat gggattaccc gttatcaaaa aaacaaaaac
    15661 tggatattcc acagccgtag atgtactgga acagcttcgt gaacaagcac caatcgtaga
    15721 ggatatcctc acctatcgtc aaattgcaaa aattcaatct acctatgtcg agggactgct
    15781 gaaagtgatc caatcagatg gaaaagtgca tacccgttat gtccagacat tgacccaaac
    15841 aggacgatta agctctgttg atcctaattt gcagaacatc ccgattcgat tagatgaagg
    15901 gagaaagata cgtcaggcat ttgttcctag agaaaaagac tggctgattt attcttctga
    15961 ctattcacaa atcgagttac gcgtacttgc gcatatctca aatgatgaac atttgaaagc
    16021 tgccttccta gaaggtcaag atatccatgc cagcacagcg atgcgtgtat ttggaatcga
    16081 aaaggcagag gatgtcacac caaatatgcg taggcaggca aaagcagtga attttggtat
    16141 cgtgtatggc atctctgatt acggactttc acaaaatcta ggaatcagcc gaaaagcagc
    16201 acagcaatac atcgatacgt actttgaaaa atacccaggt gtcaaagaat atatggaaag
    16261 aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg ctgtatcacc gccgtcgata
    16321 tctgccagac atcaattcaa gaaattacaa tatccgttct tttgcggaac gaacagctat
    16381 caatacacca atccaaggaa gtgctgctga tattttgaaa atcgcgatga tcgaattgga
    16441 taaacgatta aaagaaacag gtcttcaagc tacgatgctg ttacaagttc atgatgaact
    16501 tgtcttcgag gtaccggaaa aagaacttga atcattagat aaattggtga aggaagttat
    16561 ggaacaagct gtttctcttc atgtaccact gatcactgac agcagttggg gaaaaacgtg
    16621 gtacgaagca aaataaacat tgtttaagac agagcaatat aatgaaggtg aaaagaaaaa
    16681 atgccagaat taccagaagt agaaaccgtt cgtaaaggat tggaacggtt agtagtagga
    16741 aaaacgattc aaaaagttca ggtcttgtgg cccaagatca tcgagcaacc agagacaccc
    16801 atctttgagg cttctcttgt tggcgaaacc atccaatcca ttggacgtag agggaagttt
    16861 ttaatttttc atctcgatca ttgtgaactg atctcacact tgcgaatgga aggaaaatat
    16921 caatttacga aagaaaatac accaatcgat aaacatacgc atgtactttt cttttttgag
    16981 gatggaagcc aacttcgcta caacgatgta cgaaaatttg gtcgtatgac aatcgtcgaa
    17041 aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg gacctgagcc tttgccagat
    17101 agcttcttat tagcagattt cgcaaatgga ctgaaaaagt cccacaaagc aatcaagcca
    17161 ttgctgctag accaacgtct agtgactggt ctaggtaata tttatgtaga tgaagcatta
    17221 tgggaagcga agatccatcc agaacaaccg gcgaacacac tgcgccccaa agaggtagaa
    17281 caattacgtc tatcgatcat tgatgttttg gatcgtgcaa tagaagccgg aggtaccacg
    17341 attcgcagtt atttgaatgc attaggagaa tcaggcggat ttcaagtagc actgcatgtc
    17401 tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc cgatcgtgaa aacaaaagtg
    17461 gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc taaaaatcgc aaagtaggat
    17521 ttgttttagg tttgacgggt ggaatagcta ccggtaaaag tacggcagcc aaagtctttc
    17581 agtctcatgg atttcctttg atcgatggcg atgtgattgc cagagaaact gttgagcctg
    17641 gaacgcttgc tctgaggaaa attgtatcag tgtttggcca agaggtattg cagcctgatg
    17701 ggcaactaga tcgagggaaa cttgggatga ttgtttttcc atcaaaggaa aaacggcaaa
    17761 aacttgatca gctattagat ccgtttatcc gaaaagcgat aaaagatcaa atcgcctctc
    17821 taagttccaa tcatccttta gtaattgtag atatcccatt gctctacgaa ggtcattatg
    17881 accactatat ggatgcagta gctgtcgttt atactacgcc agaaactcag cttcagcgtt
    17941 tgatgaaacg taatcaactg acgctagaac aagcgcagca gagaattagt agtcaacttc
    18001 caatcgaaga aaaaaagcag cgtgcggata ttttgttcga caacaatgga acaaaagaaa
    18061 aacttgtggg acaaatcgaa aattggcttt ttgctaatcg ctttctttga gtatctgtgt
    18121 caattttgct atcctgagta tatagaaagt gagggatagc gatgggatca ttaaaaaatc
    18181 aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg cgagatgctc aataaagaaa
    18241 atctaatcga tgaaggtcgt acgcaatcag aaacagcaga agaaaaagaa aagcagtacc
    18301 aagcaagaat cgatgaaata gaagaccagc aggcagaaga agaagcgaag cggcaagatt
    18361 tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa aacaccgctc ccagaaagtg
    18421 accacattcc cgatgagcaa aaaatcgagg aacagcgaat gaaacactat acaggaatcg
    18481 aagagaagtt ataaaatagt aaaaaatcga gatgtgactt gaagcagatg agaaggtgtt
    18541 catctgcttc aagttttttg tgctagatga ctaaatcagc ttttgtgtaa gttatttctc
    18601 agtaatcaag gtgatcagta ttggaaaatt gaaatttagt aaggacaggg gacacaatta
    18661 cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt ttgggtagga cagtcgcgtt
    18721 tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga aagacacatc ctttatataa
    18781 aatgcgatgc aagaggtgag acaaaatgag atgtcccaga tgtcaacata ataattcacg
    18841 agtaatcgac agccgtcaag cagatgatgg acgtgcgatc cgtcgccgtc gcgaatgtga
    18901 gaattgttcg ttccgattca ctacatttga acgaattgaa gctgctccgt tattagtaat
    18961 caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa attttaagag gactcatccg
    19021 ttcagcagaa aaaagacctg tagctatgga acaaatggaa caaatcgtca accatgtcga
    19081 aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg aatctaattg gtgaatatgt
    19141 gatggaagat ttagttgatt tagacgaaat cgcttatatt cgttttgcaa gtgtttatcg
    19201 ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa gatatcgtgg ataaagcaaa
    19261 agaaaataat aaagagaata ataaagaaaa caattaagaa acaaaaagga ggcatttcct
    19321 tggaaaatgc ttggaaagag cttcaaccaa aaaatattta ccaaggaaga aaagcggctc
    19381 cgctgagaga agacagcaag aacgccttgc tgtatcttta tcagccacta atcggcggag
    19441 aagccttgag tatctatttc accctgttat cagaaatctc tcaagaatct ggcaaaggtc
    19501 ctgaagggat gcatgcagat ctgcttagta gtctaggctg cggtatcccg caattttatg
    19561 atgcaagaaa aaagctagaa ggaatcggct tactggatgt ttattttaaa gaagatagtg
    19621 atctgggacc tcgttttatt tatgagctgt tagaaccact cattcctaaa gcatttttca
    19681 aagactctgt tctggctttt cttcttctgg aaaaagttgg tgaacgacga ttccagaagc
    19741 ttacccaatt ctttgaacct aaaaggcata cagcagaggg atatcaaaaa atcacaaaaa
    19801 aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc tgctaatcaa gaaaacatcg
    19861 aatcgctgaa taaatctttt gaagaatctg aatcagtagc attacttgaa gcagattcga
    19921 cattcgattg gcagtttttg aatgattggc tggcaaaaaa acatattgca agtcttgatg
    19981 atccaatcat caaacaagtc gacatgtatc atcagcttta cggtttcgat gaattgacac
    20041 ttggcgaact gatcagtcaa tcttatgact ttagtgaaca aaaagtatct gaaaaagaac
    20101 tgcaaaaagc cattttggca atgcagagac gtccggtgaa gcaaacaaaa gaaacagttg
    20161 cggtttctga aaacagtaat gaaccctcgt caaacgaaca tttgacaaac gcagcgcttc
    20221 agctgataaa agaagcacag agcattccac cgatgaaata tctggaagca atcaagtcag
    20281 aaaaaggcgg atatgtttct aaacaagaaa actggttact gcaagatctt gtttctcaat
    20341 caggtctttc cagtagcgtg atcaatgttt tgctgaatta tgtactagtg atcaagaata
    20401 atgctagctt aaatgccagt tatgtaaata cagtggcaaa tgaatgggca caaaagaaaa
    20461 tcgccacagc agaagaagcg atccagcaca ttcggcaaat aagtaatcaa gcaaaacaat
    20521 caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg aaatgttcga cgggaaaagc
    20581 ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat cagcagcgag aaaaaagcag
    20641 agattgaccg ccgttttaaa gaatacctgg caaaaaagga gggtgatatc tagtggaaga
    20701 cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat tatcaagaaa agtatgctga
    20761 aatgatcgct gaagtcttaa atgatgcgga tgttcaagct tttctaagcg cacaccaaga
    20821 agaattgaca caagcggata ttgaaaaaag ttacgctaaa ttgtatgaat tcgtgcaaga
    20881 aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct cccggctatg agccgaagct
    20941 gacgttgaat tttcattatg tggatgtcac ttatgtacca acggaagcat tgttagcacg
    21001 gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg gatatgccaa aagatattca
    21061 atcggcttct tttgaaaact ttgaacgtac agatggacga ggatatgcga gcttagaagc
    21121 cctagatttt gtagagaaat atactgctga tccaaaaggg tatcataaag gactgtatct
    21181 agtaggaagt tttggtattg gaaaaacata tttattagga gcaacagcga gagaattagc
    21241 gattgctgga ttcaccacga cgctagtcca ttttccgaca tttgccgttg agatgaaaca
    21301 ggcaatcgga aaagatcaag tggcagaaaa gttagatgca gtgaaacgtt cacctatttt
    21361 gatgatcgat gacattggtg cagatgcgat gagtagttgg atacgagacg aagtattcgg
    21421 tgttatcctt caatatcgga tgcaggaaca gttaccaacc ttcttctcat cgaattttac
    21481 aatggatgaa ttggaacagc atttgtccgt cactcaacgt ggagatgaag agccgctaaa
    21541 agcgaaacgg ataatggaac gcatacgtta tttgacaaaa gaaatcgaaa tgactggtcg
    21601 caaccgacgc aatggttgaa cagttgacaa gtccaactga catttgacct agtagtaaaa
    21661 gcaggtcagc gaatcattat atggacagaa atgaccggag acaatcaccg caacaagtaa
    21721 tgcagaaact taaacggagg attttttatg agggctattt tttctagtat atctgtagtt
    21781 ggtctatttt ttcttttagc aggctgcggt agcgcagata gcaacacagg ttctgttgat
    21841 tcgattgatg cgggtcaaac agtaaaaaca agcagtacga aaacggagag acaaagcata
    21901 tttgtcggaa cgattgaaag tatcactcaa gtagaagcag aagaagaaac gcttcagttg
    21961 aaactcgtcg atgtcatggc caaagaagat ccagcgaata tgggaacgtc attcagcaat
    22021 gacggagtga tcttaaatgc tactgctgaa aaaataagcg tggacataaa tgaattgaaa
    22081 atcggtgaca aagtccaatt tacattagtt gagatgccga tcatgacgat gtctatccca
    22141 ccgcaagttc ctggagattc gattattgat gtttcattga acaaataacc aataacattt
    22201 tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg tatatttttt aaggagatat
    22261 gggaattttt ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa
    22321 ggagtgaagc tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg
    22381 tccctgaatc cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat
    22441 tgatattaag aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg
    22501 aatcagcacg taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg
    22561 caaatggtct agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta
    22621 tgggaggttc actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg
    22681 gacaagaacg tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc
    22741 aagaaaatca gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg
    22801 aaatcattgt agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt
    22861 tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc
    22921 ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc
    22981 aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag
    23041 cggacgcact ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg
    23101 agacccagtc ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac
    23161 attagacgtt cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat
    23221 tgctaaacat cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga
    23281 aaaaatgtat gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat
    23341 ggatattatt tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga
    23401 gagttattta gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt
    23461 ggatcatgca cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga
    23521 ttataccggt acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc
    23581 acaaccagcg attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt
    23641 tattttgccg gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta
    23701 tgaggaacct gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg
    23761 acgagtaggt gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga
    23821 agcgttccaa ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa
    23881 gcgcattgcg atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag
    23941 agcagatgtc tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc
    24001 tgggttgatt gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat
    24061 cgagaaattt gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga
    24121 aacaaataca aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt
    24181 ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg
    24241 actccatcga ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag
    24301 ttaggcttag ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca
    24361 agcaatgttg atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat
    24421 tttaatgcag taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc
    24481 gtactagatg cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat
    24541 tatgaaggcc acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa
    24601 tcagggattg cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct
    24661 cacggtacca tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa
    24721 ttcgatgttg agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt
    24781 gagttgcagt atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat
    24841 gtacatccag ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt
    24901 cataatcagc ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc
    24961 catttgatgg cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat
    25021 catgatcgtg aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg
    25081 aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg
    25141 aaagaaatca ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag
    25201 cttggtatct ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct
    25261 tacatgggta ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc
    25321 gaatttgttt ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa
    25381 ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg
    25441 atggaatgtc atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc
    25501 ttttttgaag agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa
    25561 gttcagggaa agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa
    25621 agcagccaga attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat
    25681 tagtcaagaa agcagaagcc atagaagctg atactttagt aattataggt ggagacggca
    25741 cgattcatca tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac
    25801 ttccaggtgg aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag
    25861 aagctgctga cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg
    25921 atgaagtcat tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca
    25981 gtcaagaaga aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat
    26041 tgctgaagaa gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa
    26101 aatcccaact gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg
    26161 actcagcgac cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt
    26221 tccgatttat tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca
    26281 ttacttattt cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc
    26341 gaacggatgg agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat
    26401 taacagttta ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt
    26461 ggaagaagag taatggatgt attgttacac aatcctttgt ggttttacct tttcattgtg
    26521 aatttttact tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg
    26581 cgtgttcctg agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg
    26641 atctctcgaa aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct
    26701 gcaggtgtac ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc
    26761 gtctaaagga acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc
    26821 cattatttat tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac
    26881 cagtgttgtt gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg
    26941 gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt
    27001 cttgacctct agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat
    27061 ctcggagatt tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac
    27121 agcactccat atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt
    27181 catccaattc tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg
    27241 tatccttttg acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt
    27301 tcaaatattt tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg
    27361 ggtggataca tgcaatcagc agatctactc tctaagggaa acggtccaaa ccattatcca
    27421 agacaggact gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta
    27481 tgtacttcct tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat
    27541 ttcgtttatc agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg
    27601 ttcctttgaa tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt
    27661 ttcttcgctt ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt
    27721 gtatgtgat
//
LOCUS       DACDHJ010000016        27356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDHJ010000016 DACDHJ010000000
VERSION     DACDHJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.016"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IV223_000569"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IV223_000569"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8710877.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2259..2510)
                     /locus_tag="IV223_000570"
     CDS             complement(2259..2510)
                     /locus_tag="IV223_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8710878.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2656..3279
                     /gene="lexA"
                     /locus_tag="IV223_000571"
     CDS             2656..3279
                     /gene="lexA"
                     /locus_tag="IV223_000571"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8710879.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3459..3677
                     /locus_tag="IV223_000572"
     CDS             3459..3677
                     /locus_tag="IV223_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710880.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3966..4478)
                     /locus_tag="IV223_000573"
     CDS             complement(3966..4478)
                     /locus_tag="IV223_000573"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8710881.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4492..6789)
                     /gene="recJ"
                     /locus_tag="IV223_000574"
     CDS             complement(4492..6789)
                     /gene="recJ"
                     /locus_tag="IV223_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8710882.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6931..7365)
                     /locus_tag="IV223_000575"
     CDS             complement(6931..7365)
                     /locus_tag="IV223_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8710883.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7375..8163)
                     /locus_tag="IV223_000576"
     CDS             complement(7375..8163)
                     /locus_tag="IV223_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8710884.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8160..9101)
                     /locus_tag="IV223_000577"
     CDS             complement(8160..9101)
                     /locus_tag="IV223_000577"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8710885.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9278..9865)
                     /locus_tag="IV223_000578"
     CDS             complement(9278..9865)
                     /locus_tag="IV223_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8710886.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10086..11399)
                     /gene="obgE"
                     /locus_tag="IV223_000579"
     CDS             complement(10086..11399)
                     /gene="obgE"
                     /locus_tag="IV223_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8710887.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11687..12697
                     /gene="gap"
                     /locus_tag="IV223_000580"
     CDS             11687..12697
                     /gene="gap"
                     /locus_tag="IV223_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8710888.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12782..13717)
                     /locus_tag="IV223_000581"
     CDS             complement(12782..13717)
                     /locus_tag="IV223_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8710889.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13903..14862
                     /locus_tag="IV223_000582"
     CDS             13903..14862
                     /locus_tag="IV223_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8710890.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14948..16042)
                     /locus_tag="IV223_000583"
     CDS             complement(14948..16042)
                     /locus_tag="IV223_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8710891.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16231..16992
                     /locus_tag="IV223_000584"
     CDS             16231..16992
                     /locus_tag="IV223_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8710892.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17107..17547)
                     /gene="fabZ"
                     /locus_tag="IV223_000585"
     CDS             complement(17107..17547)
                     /gene="fabZ"
                     /locus_tag="IV223_000585"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8710893.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17767..18522
                     /gene="fabI"
                     /locus_tag="IV223_000586"
     CDS             17767..18522
                     /gene="fabI"
                     /locus_tag="IV223_000586"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8710894.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18632..19645)
                     /locus_tag="IV223_000587"
     CDS             complement(18632..19645)
                     /locus_tag="IV223_000587"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8710895.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19810..20730
                     /locus_tag="IV223_000588"
     CDS             19810..20730
                     /locus_tag="IV223_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8710896.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            complement(21010..21510)
                     /locus_tag="IV223_000589"
     CDS             complement(21010..21510)
                     /locus_tag="IV223_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8710897.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(21617..22135)
                     /locus_tag="IV223_000590"
     CDS             complement(21617..22135)
                     /locus_tag="IV223_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8710898.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            complement(22136..23491)
                     /gene="rph"
                     /locus_tag="IV223_000591"
     CDS             complement(22136..23491)
                     /gene="rph"
                     /locus_tag="IV223_000591"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8710899.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            complement(23494..24315)
                     /gene="racE"
                     /locus_tag="IV223_000592"
     CDS             complement(23494..24315)
                     /gene="racE"
                     /locus_tag="IV223_000592"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8710900.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            complement(24477..25133)
                     /locus_tag="IV223_000593"
     CDS             complement(24477..25133)
                     /locus_tag="IV223_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8710901.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            complement(25149..25796)
                     /locus_tag="IV223_000594"
     CDS             complement(25149..25796)
                     /locus_tag="IV223_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8710902.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            complement(25809..26624)
                     /locus_tag="IV223_000595"
     CDS             complement(25809..26624)
                     /locus_tag="IV223_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8710903.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            complement(26638..>27356)
                     /locus_tag="IV223_000596"
     CDS             complement(26638..>27356)
                     /locus_tag="IV223_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8710904.1"
                     /translation="HVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKSTMLRCING
                     LETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKLAPIKVLK
                     QDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEMLLFDEPTS
                     ALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLEDSRNVRD
                     FFENPNEVRAQQFISKVINH"
BASE COUNT         7855 a         5919 c         4793 g         8789 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggttttttta cctttatttc ttacactttc attctataag
     2221 atgtcacacg aaaaagcaat ctttttcagt attttttact aaaagttgtc atttcggttg
     2281 tgtaagcctt ttttcttttg gatttctttt aatttttccg gggtcacgtc attgccctca
     2341 ggatcgacga ctttcattcc ttcgatatga tgacgcatac cggaacgaaa agcttccaaa
     2401 tactctttgc gcaagttgat ttgttcttgt tcttcggctt ttgagagacc attttccttc
     2461 tttttacgtg caagttcatt gatccgctct attttttcag gtgacagcat gctcagccct
     2521 ccttttactc tccttcatat tatataaaaa ctgaaaaaaa acaactagat aaactaaata
     2581 aaatacgaac attcgtttga tttagcgctt tttatatggt actatttatt tatcaagtga
     2641 aagaaggtgt accttgtggc ccgacaaact gaatcaagac aaatcgaagt cttacgatat
     2701 atccatgaac aagtaaccca aaaaggatac ccgccgactg tccgtgaaat cggggaagct
     2761 gttcagcttt cttctacttc tactgttcac ggtcatcttg cacggcttga aaaaaaaggg
     2821 tttatccaaa gagatccgac gaaacctcgt gcaatcgagt tgacaaaaca aggtttagaa
     2881 aagattggga ttcgtccaac aactattcca gtacttggcg tcgtaacagc tggagaaccc
     2941 attttagcag tagaggatgc ttcagacttc tttccaatcc ctcctaatct ttcttccgaa
     3001 gaaggcagtc tctttatgct gacaattcgt ggagatagta tgatcaatgc tgggatattg
     3061 gatggtgaca acgtgatcgt tcggaagcaa gaaactgctc aaaatggcga tattgtcatt
     3121 gcaatgactg ccgaggacga agcgacttgc aaacgattct ataaagaaaa aaactttttc
     3181 cgtctgcagc ctgaaaacga cttgctcgaa ccaatcatct tagatcaagt atctatatta
     3241 ggacgagtag taggacttta tcgtagtcat atctattaat tgctactaat taaaagaaaa
     3301 ctttttaaaa agagtttgag acgttggtct caaactcttt tttcgcccgg gttcttcttc
     3361 tttcaaattt ttatcacttc cctctttaca aaccgaacaa atattcgtat actaaatata
     3421 cgaacaattg ttcgcataaa ttaaagaggt gagaattcat gaaatcaatg agacgtcttg
     3481 gcttagtatt aactgttttg tttttaggta ttattattgg taatttcggc atacgtccag
     3541 ctttacttgt gtttgttccg ttatttactt tgtggttcat gttgtgggat gaaaagaagt
     3601 accgtcaatc tgagcggcgc agaattcatg aagatcattt ctatcatgag ccctactaca
     3661 gatccaggca atcataataa ggattgactg ctaattattc aaaagacaag aagttaaact
     3721 tttacggggg tgtgacaaaa atcggtcaca tccttttttt cgaataaacg gtgttctaaa
     3781 gccagttttt cggtattaag tcaaattgcg gaaaaagtga ttttttaaac ttctactggt
     3841 ctttgcttgt ttccatttat aaaaaaatag cgaccctcaa aagtcagata gagaatctga
     3901 cttttgaggg tcgctttaaa atcgcttctt taagaataac atcaagaata gcttcttttt
     3961 tctgcttaat attccattaa tgtaatgatg tcatatcctt tgattttgtc gcgtccatgg
     4021 agatccatca attcgatcaa aaatgcgcag cctacgacta caccgcctaa ttgttcgatc
     4081 agttcgatcg tcgctttgat tgttcctcca gtagctagca aatcgtcaca aatcagcaca
     4141 cgttgacctg gtttaatggc atccttatgc aatgtcaatg tatctgttcc atattctaat
     4201 ccataagtca cttcgatggt ttctcgaggt aacttccctt ttttgcggac tggggcaaaa
     4261 ccgactccta actcataagc aacaggacat cctacgatga atccacgggc ttctggccca
     4321 actaccatat caattcgttt ttcttttgca taatttacga tttgtttagt agcttcccga
     4381 taagcgtcac catctgccat cagtggggaa atatctcgaa acacaattcc ttttgacgga
     4441 tagtcgggaa tactggcaat ataatctttt aaatccactc tttgtttcct cctaggttgt
     4501 taaccatttc tttatggtag gaatatcgct caaaagcaaa aattcctcaa ctttgatttt
     4561 attgagccgt cgctggtata atctgctctc agtcaaagga tgactttctg ggctagcgac
     4621 tttttgtaaa acaccgttct ctattgtaac aaatttcaat tcaaaaaaca cctgtatcat
     4681 aaaaattaat aatttttcag ggatacgcaa atattgagct attgctttta ttttatgacg
     4741 tatatcaatt cgttcttgag aatggatcaa tttaaacaat tttgcgtact gttctcttgt
     4801 gcctacccca ttcaaatagg cctcatcagg tgaaatcccc atcagataaa cacgattgaa
     4861 tgaataagac cgaaaaactt ctttcagatc cgctaaatca tctggacaat ccataacaac
     4921 tagagagttt ggttttcttt tttcaattat tgcctttaca gaatctagtc cttcgaaaac
     4981 aatcgcagct tcctggataa tctcaggcac gaatttcaac gtcgaagaat caaatgctaa
     5041 ataaaggcta ccttccgacg ttcggctctg ttcacggaaa cgttttgccc gccaatcaaa
     5101 tacttgcaga ccacccacag aaaaatcaga taccatcagc tgcggtttct tacgcccgtt
     5161 ccattcattg atcgaaagct ttcctaccac atctacctga ttattcagca gttcttcttc
     5221 ttgagtacca aaaccaaaag cgaccgcatc aagctgactc gatgcatcgg aaaggacaaa
     5281 tttcagatgc tgttggtttg ctccgattct tttgatattt tctgcttgga cttgtcgaaa
     5341 taaaaactga ggcaaaggat tatccgtacc aaatggcgcc aatagtttca gctcatcaat
     5401 tcgctctact gttacttcat ttggcagcaa tacttcatca attctcaact caggccctct
     5461 catcaagtca atttgatgat cgactatata ttggttcatc ttctcctgta gtatagtgac
     5521 attttcactt ggcatcgtta aaccgactgc tgcatgatgt cctccgaagt aagtaaataa
     5581 atctcgcatc tggtcaagca tttcaaaaag attcaaggct tcaatactac gtccagatcc
     5641 tttagctgat ccatcttctt ttaaagtaag gacaagcgtt ggtttacccg tttcattcat
     5701 tattttccca gcaacaatgc ccaaaacgcc ttcatgccat cctcggttta ccaatagatg
     5761 gatatgattc tccttattga ccatcgctaa tgcttcttgt gttatctgct ctacgattgc
     5821 ttttctttct tcattgatct cattcaattt ttttgcttga acggatgctt cctcttcatc
     5881 aaacgtcgca agtaacgaga cggcaggacg tggatcgccc atccgaccga ttgcatttag
     5941 tctgggagca atcgaaaaac cgatcgttgt ttcgtctgcg tccctcattt ttacgccgct
     6001 ttcttcaaat agcgcctgaa gtcctatccg ttctgaatga tgaatggcat cgattcctaa
     6061 tgaaacaagg attcgattct catcagtcaa tgaaactaaa tcggcgatcg ttcctatcgc
     6121 tactaaatcc aaaaattctg caggcggttc ttccaataat gcagaagcta ctttgaatgc
     6181 tactccgact cctgccaaat ctggaaatgg atagtgcccc gctggatgac gagggtgaac
     6241 aatcgcataa gcgtctggta aattctcagg tagttcgtga tgatctgtaa tgatcacgtc
     6301 tacaccagct tgctgagcgt atgctaccgc atcatttcct gctaccccgt tatcaacagt
     6361 gatgatcaac tggattcctt cttcgatctt ttcttggtat accgattggt ttggtccata
     6421 cccatgctca aatcgatttg gcaggaatac ttctacttct gcaccaagta attccagtgt
     6481 ttctttcatt accgttgcac tggtgatccc atctgcgtca taatcaccgt aaacgaggat
     6541 tttttctcca ctgattacag cttcttggat tcggctgatt gctttatcca tatcgtgtat
     6601 cagatacgga tcatgtaatt cttgttcctt tggatgtaaa aatcggttga tatccttttc
     6661 ctcatggtaa cctctttgcc acagtaactt accgacaaat ggagataatt tcagttcacg
     6721 gatcatttgg agaaaggatt cagaaggctc tgtctcgctt aacttccaat caaaatttgc
     6781 ttgtctcact ttctcactcc taacttagtt attatagcaa aggaaatcag caaaaaaaag
     6841 cagaaagaaa gaataaaaac agatcagatc cgctagacag atgttcagca ggacctgatc
     6901 ttttgtacga ccatttatga aatatcccta ttagtcaaaa taattgaagc tttgtttggg
     6961 cgattgagtt ttttgttctt ccggcgcttt caattgactg atttgtgcgg ttttcgcttg
     7021 taactccgcc tgataagcag cttccaactc cgcacgttcg tttctaaact cgcgttgctg
     7081 ttcttctttt gcttcttcaa ttttcttttg aagttctgtt tgatattctg acatttcctg
     7141 agtcagctgt ttgatttgct ttttttgctg ccatagtgtt gccgaagcag tcaaaaatac
     7201 aatcagtgca ccaatcagcg cagaaccgat gatcactaag atcaatggcg cagaaaagga
     7261 agtgaagcca aaacttacag gaacattttt attgttcaat acagcaaaaa gaacaataat
     7321 caaaactaaa acaaatccaa gaatcacatt gctttgcttt ttcaattcct tcacctactt
     7381 tttgttaaaa attccgctgg ctaaataatc accaatcgtt ggaaacagcg tataaaattt
     7441 tgatgcaact tctaaaataa acggttgatt gatttcccgt tttggatgtc tcatattgcg
     7501 gacgatcatt tttgccaact tggcaggttc taaaacgatt tgtccgactt tttccaaata
     7561 gcttccgctt ggatcagcct tatcgaagaa atttgtctcg attggacctg gattcactgt
     7621 cgtaacagct acgccaagtg gtttcaattc taatcgcaaa gcgttggaga agccaaggac
     7681 tgcaaatttc gtggcagaat aaaccgttga ttttgcagtt gccatttttc cagccataga
     7741 ggcgatatta atgatatgac cttgtcttct ttctgccata tcgatcgcaa atttttgtgt
     7801 caaaaccatc attcctaaga tgttgacttc aaacatatcg tatgcttttc caaggtcgaa
     7861 tgtaaggaag tcttcgaaaa tcccaaaacc cgcattgttt accagaatat cgacgcgccc
     7921 gacttcttcc atcacctttt catataattc gtccacgctt tcaggattgc tgacgtctac
     7981 cttgaaagca taagcctctt tgccgctcaa gcgtctgcac tcgtctctga caccttctac
     8041 aaatgccatt cttcgtgcgc aacttataac gattgctcct tgctttgctg cttcataaca
     8101 gatttgtgcg cccagaccgc ctgaactacc tgttactaaa acaactttat cttctaaatt
     8161 catgcctctt cctcctcgcg aaatggaatc tcaatgctat caaaatcttt cataatcgtc
     8221 gtgttttcaa acacttcttg tgcttctttt tcaagctcta aagctgcttt tcctaaatat
     8281 ctcgcactga tatgagtaag tagcagccgt tttgcctgcg cttcttttgc aacttcagca
     8341 gcttgatgag tcgtggaatg gaaatagttg tgcgccattc ttgcttcatc tttattaaat
     8401 gtgctttcat gtaccagaat atcgctattt tccgcaagca caacggaatt atgtgttttt
     8461 ctcgtatcgc ctaaaatagt cacgattcgt ccttttttat ctggacctac gaaatctttt
     8521 ccattgatcg tccgcccatc ttctagttgg atcgtttcac cttgcttcag tttgccgtac
     8581 agtggtccag ctggaatacc taattctttt agacgatcca cctgcaattc tcctttatga
     8641 tcatgttcaa ccacacgata accaaaactg tcgatcccat gatttagttt ttttgtataa
     8701 acagaaaatt gctgatcagt aaaaatcggg gctgtttcat ttagctcaat gaatttcagc
     8761 ggataactta aacgcgtttg cgaaatacct aaagagactt ttatgtattc ctctatccct
     8821 tttggcccgt aaatctctaa tggcgtatcc ccaccttgaa atgaccggct gctgatgagc
     8881 ccgggaagtc caaaaatatg atcgccgtgt aaatgtgtaa tgaaaatttt accgattttt
     8941 ctcggacgaa tagtcgttcg caaaatctgc atttgcgtgc cttcgccaca gtcgaacaac
     9001 caaacttcat tgcgctcgtc taaaagtttc aatgctatac ttgtcacatt tctatgtttt
     9061 gcaggaacac ctgcacctgt tcctaaaaat tgtaattcca taatctacct gactttcttt
     9121 aagttcctct ttaattttag ggtaaaaaga agggataatc aaccgtaagt cgagaatggg
     9181 ttcaaattaa aaataacgtt ctaaagcgat agcgttttct ttgaaaatta ctataaaaac
     9241 agcagcgaac ccgtttgaga tcctctgctg ttttttatta atttgaagat ccagaacttt
     9301 cattgtcgtt ggaagtttgc ggatgtttgt ccttccaagt cttttgtgca gcttctttcg
     9361 catgattttc agcttctttc gcatcatgca catctttttc gacatcttca taatccaagc
     9421 gagtaatttc tccgcctaat tccatcatta ccaattgatt cagtgggcga ttgtcctctt
     9481 catcagcaat caagatcagt cctgtagctc cttcaccgat tttatttcct acaaagtcga
     9541 acacggtctg ggcatctttt atttctttgg catcttgtgt cgcaccgatc attcctccgg
     9601 caaaccatcc taataaaatc cctagcggac cacctaatac accaaccaac attccgatta
     9661 atccacccgt agaagatttg ttattaccgg taaagtcgat aaaatcttct attttgaatt
     9721 gatgtgtccc attaggatca tggctgacaa ctgccatctg ttctccttta atcgaacgaa
     9781 cagcatgtaa ctttttgatt tctgaaaatg cctgatatgc ctggctttct acttgaaaat
     9841 ttaaaatgat tactcgtttg ctcattaaaa acgccccttt cataccttct tgttatttat
     9901 tttaccttta attgaaaaat aagaaaagaa aagcactctt tttttccaat aaaatagggc
     9961 tgtgacaaaa gtcgtatcac agctcctttt tccgaataaa cggcgttctt gaagctgatc
    10021 acttctgtca caacctccct taaatctgaa tctatatttt ttggtatttg tttttatttg
    10081 cttgcctatt cgacaaattc aaattcaaac tcaccgatac gtacaaggtc gccatctttc
    10141 gcaccgcgtg cccgaagagc ttcatccaca cccataccac gtaattgacg agcaaaacgc
    10201 atcactgttt catcatgatc gaaattcgtc atttggaata atttttcgat cttctcacca
    10261 gataagatcc acgttgcatc tgaatcacga tcgatttgga actcaggacc ttctggattg
    10321 aatccatagt gtacagtttc ttcttccagt tcttcttcgt acaatgggaa ttctggcgtt
    10381 acttctaaca aatctgctgt agcattcaat aacgcgtcca atccttgtcg agtcactcca
    10441 gaaattggaa atactgggat gtcatcagca aattcatctt ctttttcctt gttcagctgt
    10501 tctttgaatt tcactagatt ttccggtgca tccggcatat ccattttgtt tgccacgatg
    10561 atttgcggac gctctagcaa gcgtaagtta taagtagata attctttgtt gatggcaaga
    10621 tagtcttcat aaggatctcg tccttccatc ccactcatat cgatcacatg taaaattact
    10681 cgtgttcttt cgatatgacg aaggaattgt gtacccagcc caacaccttg cgaagctcct
    10741 tcaatcaagc ctggcaagtc tgctgcggca aaacttctgc catcacttgt agtaaccatt
    10801 cctagattag gtacgagcgt cgtgaaatga taagcgccga tttttggacg agcagatgaa
    10861 ataacggata gtaaagtcga tttcccgaca gatggaaaac ctacaagtcc aacgtctgcc
    10921 aatactttca attctagttc gatttttcgc tcttgtcctg gttcaccgtt ttcagcaatt
    10981 tccggtgcag gattccgagg agaggcaaaa cggatattcc ctcgtccacc gcgtccacct
    11041 tttgccacaa ctagtgtttg accattttca attaaatctc ctaaaagagc accagtctcc
    11101 gcatctcgga cagtggttcc ttgtggtact ttaacatagg tatcttctga accacgaccg
    11161 tgcatccctt tactcatccc attttcacct ggctgcgctt tgaaatggcg attgaaccga
    11221 aaatccatca atgtacgtaa cccttcatcg acgatcaaga tgacatcgcc gccacgacca
    11281 ccgtctccgc cagccggtcc gccgtcagga acgtattttt cgcgacgaaa tgcaaccatt
    11341 ccgtctccgc ccttgccagc tttcacatcg atcgttactt gatctaaaaa catggacata
    11401 attaagtcct cctattattc atttatttat ttgactgcct gaatttccac aataaacaaa
    11461 agtcttactt attttatcgg tgaaatctga ttttgtctat catttcagca aaaagattgt
    11521 ttaccttctt ctttttttat catttggtaa ggtttccaac cttttttctg ctaccaagta
    11581 taattttttg acaaaatatt ctttgcgtag cagaataaca gtgtattaat tgtattaatt
    11641 gaggatgccg tttcacagca cccctaataa aggaggaact tttgatatga cagtaaaagt
    11701 aggtattaac ggatttgggc gtattggccg tctggctttc cgccgcatca aagaagtctc
    11761 agatgatatt gaagtagtag caatcaacga cttaacaagt ccaactatgt tagcacaatt
    11821 attgcaattt gactctactc atggtactta tcctggcaca gttactgcaa cagaagactc
    11881 aatcgtagtg gatggcgaag caactcgtgt ttatgcagaa cccgacgcaa gcaaaatccc
    11941 ttgggcaaaa gaaaacggtg tagatatcgt actagaatgt actggttttt atacatctga
    12001 agaaaaagca aaagcgcatt tagatgctgg tgtcaaacga gtagttattt ctgccccagc
    12061 tggtgctatg aaaaccatcg tctataacgt caatgatgat acattagatg aaaacgacaa
    12121 aatcatctca gcaggttcat gtacaacaaa ctgtttagca cctatggctt atttcttaaa
    12181 taatgaattt gggatcgaag ttggtacaat gacaacagtt catgcctaca cttccacaca
    12241 aatgctctta gatggacctg taaaaggcgg taatttgcgt gctgctcgtt cagctgctga
    12301 caatactatc ccacattcta ctggtgctgc gaaagcaatc ggcttagtta tccctgagct
    12361 acaaggaaaa ttacaaggac acgcacaacg tgttccagtc gttgatggtt cattaactga
    12421 attggtttca gtattgaaaa caaaagtaac tgctgatcaa gtaaacgaag caatcaaaaa
    12481 acacacagtt gacaatcctt cattcgggta tgatgaccgt caaatcgttt ctagcgatgt
    12541 cattggaaca actcaaggtt cgatctttga cccaacgcaa acagaagtaa caactgctgg
    12601 tgatttccaa cttgttaaaa ctgttgcatg gtatgacaat gagtatggat ttacttgcca
    12661 aatgattcgc ttactagaaa aattcgctaa cttgtaattt gatagatcaa tcgataaaag
    12721 aagcctttga ctggatccag tcaaaggctt ctttcttttc atcccctact tttaaactcg
    12781 ttcaatgaac atattggctt aaaaattgtt ggactacttg ctgatatttt tcaggatctg
    12841 tcttgtatgc ttccgcatgc tcggctcctt ttacaacata cttttctttt ggtcccttcg
    12901 ttgcacgata gaccttatct aacatttcaa atggtacaaa tgtatcttcg tctccatgta
    12961 taaagagcat tgggcgctgg tttttcttca attgttccac tgcatttgct tcaccgaaaa
    13021 agtagccagc acggactttt gtcactagac tcgttacttg cataagcgga aatgctggaa
    13081 gactaaacat atcgtctagc tgataagcaa gctcatcatg aacggaagaa taaccgcaat
    13141 cttcaatgat tgctttcaca ttatctggta gtttttctcc gctagtcatc ataactgttg
    13201 ctgctcccat gctgacaccg tatagaacga tttcttgcga ttttccattg ttttctagca
    13261 ctttgtttat ccactgtaca tagtctttac gctctggcca tccaaatcca atgtaatccc
    13321 cttcgctttt tccatgtcct cttgcatcag gaacaaggac attgtaaccc atatcatgaa
    13381 acatctttgc gtaattggtc atcgtttcag cgtttcccat atagccatga gccacaagag
    13441 ctgttttttc acttttcgtt tcagcaggaa gataaatcgc tgataatttc agaccgtctt
    13501 ttgaagtgat ggtccaatcg cttcggtttt cttttgaact aaaccattta tgtgcatccg
    13561 tttctttgga tgtctgtgtt gtaccaggtg tatcttcgtc taaaaagtct ttctcagaag
    13621 gcacaacagc ataattataa aaataattcc ctgcaaacac taacgcaaca atcagagcta
    13681 aagcgacgcc taaaaggatt ttcatccaaa gtttcataaa tcatccatct catttctctt
    13741 ttttcatata accttgaatt agtataatca aagaatctct actatgcaat gttcattttt
    13801 ataacccgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
    13861 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
    13921 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
    13981 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
    14041 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
    14101 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
    14161 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
    14221 acactttatc gtttattttc taaaggaata tttgatattg acacactacc gatgaaaggt
    14281 aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca gcgctcaatc
    14341 catgatcgac ctgataatta tcctgatttc aattctgagt ttggtcacct tgaaggtgat
    14401 acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag attatctaaa
    14461 gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac tgcccttaat
    14521 caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa
    14581 gaattttcta actggaaagc cattagtaac caacatgata ttgatatata ttttgcggac
    14641 cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct gcgtcgtaat
    14701 ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc cagtgtcagc
    14761 aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat cgaaatattt
    14821 ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat aacctataaa
    14881 aaaacaaaat aaaaaagcag aaataactgg ctatttctag cggattattt ctgcaataac
    14941 taattatcta ttttttattt tcaattaatt taaagcttga ccatggtttc aaaaagtcaa
    15001 ggaggaataa ttcgtcatcg gagattcttc ctacaacatt gacacgtccg tcgtttttca
    15061 tatccttcaa ggcgatttgt gtttctcctt tgtattgacc gtacccttca ttgtcaatca
    15121 gtacatcccc atgatgcata tctcgcgtat tatgaggcgg gaaatcttta tctttgtagt
    15181 atacacgagt catcgttgaa cgaaggatat attctgagcg atcccctcga taactatgaa
    15241 ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc ttcttccgta tctactttca
    15301 atgttggata atccgcgtta aatgcttctg ccatttcttt caattcttct tcacttgcgt
    15361 acgcatttcc aatagaaata tcatcaatca atcctgtaag gattaaatgc ttcacttgtg
    15421 tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa gccatcttgc gtaggccaag
    15481 ggccaaactc agcagattgc gaattgacga atgccatcgt attcaaatta tattttctga
    15541 atttttctga acaaaaaaca aagtgttcgt atcctaaacc agagtatcga tgaggataaa
    15601 aattatgaga acctaataaa ttatccgtgt ttggcgaata actcatgatg ttgtcaacgt
    15661 aattcgtgcc cgaactcata ttgatctcga ttttgatacc aaaaggattg cgcgtcattt
    15721 ttgcttcttc tgctcctgtg aatccgatat ctaagcggac cccgtaggca cccatcttat
    15781 ggaaaaaaga taagtcatcg taagaaatct ccaactgttc aaataacgct ggattgatat
    15841 caaccattac ttccatgcct aggctattcg cgtaatcaac tacttcttta aattctgaaa
    15901 gaactttttc tttatcatca ttgatctgca gcaaactggt aaaaacccgt ttatacccat
    15961 acttatgagc taaatccaag tacgctttgt ccttttcaaa tgttgaccgt tctggataaa
    16021 tcgagattcc taattttccc atattaccat ttcctcctgc atcttttcgt ttttgtatcc
    16081 acttacatta tagcaaacac aaagtaaatg gtctacacct tttctttttc ttttaaaata
    16141 aatgataaat tccaataatt taagtaggta ttttccctct attttgttat cattaaagat
    16201 aacaaaatag taaattggaa aggaacagtt atgaagaaaa ataaagttat tttaagtatt
    16261 agtttagcag cagcattgct gtttgctgct ggttgttctg caaacaacac gacttcttct
    16321 agttcgtcta cagaaagttc aaaaagtaca tctactgcgg aaagcacgac tgccagtagc
    16381 gtcgatttga acgcattgga attaccacaa ttgacgaaag atgtcgccga aaatgaagat
    16441 ctggtcgaaa tggtgacgaa taaaggcaca atcaaaatca aattatttcc cgaacacgca
    16501 ccaaaagcgg ttgaaaactt tatgacacat gcaaaagacg gttactacaa cggactgact
    16561 ttccatcgcg tcatcaaaga tttcatgatc caaggtggcg atcctaaagg cgatggcact
    16621 ggaggggaaa gcatttgggg agaaggattt gaaacagaga tcaacaacca cctttataat
    16681 ataagaggtg cgttatctat ggctcgctca caagatccta acagtaacgg cagccagttc
    16741 tttatcgttc aaaatacaga tgatatgcat gatggtcttt taaaagacga ctatcctcaa
    16801 gcaatcatcg acgcttataa aaacggcggt tatccttcac tagatggcca ttatactgtc
    16861 ttcggccaag tgattgaagg aatggatgtc gtggatgcta ttgcaaatat cgaaacagat
    16921 agttcagaca aaccaaaaga agaagtaaaa atcgataaaa tcaatatact gcaagaagca
    16981 aaacaaaaat aaaaaaataa cgtctgaatc ctgcatagat gacaacgtat gctaggattc
    17041 aggcgtcttc tttttacata aaaagcaagt aagcttcctt ttgttttgct atatcaggtt
    17101 ttatagtcaa gcattttggt tgacgatgaa cgtgaactcg cactcacaga cttttttatc
    17161 atctacatat gctgcagata acgctgtacc gactgatccc ttcattttgg tgatttcaaa
    17221 atgacatttc agcacatctc ctggaacaac tttttgacgg aatttggcct tattgatccc
    17281 accgatataa gctctttttc ctagaaactc ttctgatttt aggataagta tcgaacctgc
    17341 ttgtgccagt gtttctagaa tcaacgcacc cggcatcact ggattgtcag gaaagtgacc
    17401 ttggaaaaaa tcttcattga ttgttacatt ttttgtcgca acgatctttt ttcctggttc
    17461 taaagtatct acataatcaa tataacaaat aggataacga ttaggaatca attccattac
    17521 ttcagtagca gataatacgt aagacataat taaactcctt ctttcaggca attgccattt
    17581 tcattacttt gactatcaaa gtatcatatt cctaaatatt tagcaatctt tttttaattt
    17641 aagaaaaaat agtttgacaa tcaaaacatt ttgactaata attaagaaat caagaagtta
    17701 ttttagctat tattctctat ttttttacga ctatcaaatt tttatcagaa acaaaggaga
    17761 gtcattatgt catttctaga aggaaaaaaa atcgtcatta tgggcgtcgc taacaaaaaa
    17821 agcattgctt ggggttgtgc tgaagcaatg ttgaaacaag gggcagaaat tatctatact
    17881 taccaaaatg aacgaatgaa aaaatcatta gtcaaattgg tcggcgaaga agcattcact
    17941 gtagaatgtg atgtttctag cgacgaaagc atcgaaaaag caatgaatgc catcggtgaa
    18001 tatgctgggg agatccacgg ccttgtccat gcagttgctt acgccaacaa agaagaattg
    18061 tccggaaatg tgtctgatat ttccagagac ggctatcaat tagctcaaga tatcagcagc
    18121 tactctttaa ttgctgtatc aaaatacgca caaccgtttc ttgcaccaaa cagcgggatc
    18181 gttagcatga cttatctagg ttcagaacgc gcaatcccga attataacat gatgggtatt
    18241 gcgaaagctt ctcttgaagc tgctatccgt tatttggcag ctgagttctc gcctaaagga
    18301 attcgggtca atggaatctc agctggggca atcaaaacat tagctgtcac aggtgttaaa
    18361 gactatcaaa aactgatcca gttatctgaa tctcgtacgc ctgatcagca aggtgtgacg
    18421 atcgaagaag tcggtaatgc ttgtgctttc ttaatcagcc cactttccag cggaatcatc
    18481 ggtgatatta tctacgttga caaaggtgtc catctttcat aaacttttgt tgcattcttt
    18541 aaagtaaaac atataaaaat caggaaaaaa ctttttaaag tctcttcctg attttttctt
    18601 tttacgtttt tttcaactca ttactttgaa attattccac gacttcaatc cagccttctg
    18661 gtgcttctat gtctccaaat tgaatacctg tcaattcatc atacaaccgt tttgttactg
    18721 gtcctacttc cgtttcacta taaaatacat gaagattgtt attgtgataa atcccaccga
    18781 ttggagaaat aacagccgct gtaccacatg caccagcttc tgcaaattga tctagatcat
    18841 caatgaaaat atctccttca attgcttgca gtcctagtcg ttcttcagct aactgcaaca
    18901 aggaatattt cgtgatactt ggtaagatag agggcgattg cggagtgatg aattttccgt
    18961 cttttgtaat tccaaagaaa tttgctgcac ctacttcttc gattttcgta tgtgtggctg
    19021 gatcaagata aacacaatct gagtaatcca gttcttttgc ttctgttcct ggaagtaaac
    19081 tcgctgcgta atttccgccg acttttgctg caccagtccc cttaggagca gcacgatcgt
    19141 aatcagatac aataaaattc gttggtttta gtccaccttt gaaatacgct cctactggcg
    19201 tacagaagac gacaaataaa tattctggag ctggatggac accaatgttt tcaccgaccc
    19261 caatcagtaa agggcgcaag tataatgtag cgcctgttcc atagggcggc acaaaatctt
    19321 catttgcttt taccacttgc ttgacagcat caacgaacat cttttcaggg accattggca
    19381 tcagcaatcg tctagcactt tgattcatcc gtcgactatt ttgttcaggt cgaaagagat
    19441 tgaccttgcc gtcttttcga cgataagctt ttagaccttc aaaacattgc tgaccataat
    19501 gaagcgctgg agagccttca tgaatcgtca ggtaattatc ttctgtcaat tctccttctt
    19561 cccattgtcc gtctttccat ttggcaataa aacgccaagg agttttgata taagaaaaac
    19621 ctagattgtt ccaatcgatt tgcatatact taccaccttc gttattttct ccacatttta
    19681 tcattatcca tattatttgt cattttattt tcagcttttt tccttcatta tagttatttt
    19741 tgctatttcc ttttttgaaa acttcttggt acaatacaga aagaatcaaa ttttagaaaa
    19801 gaggatttta tgttttcatt attagcaact gccggtacga cagactcttc tggcgagaca
    19861 ttgacagatc aagctgtcag caaagtaaat gcgtttcagc ggttttggga cgggattgat
    19921 tgggacgcca ttttggctgc ctttatccaa aaaagtttat atctgatttt tttgatcgtc
    19981 cttttttgga ttcttcaccg tgtgggaaaa tatctgattg ataagtcttt taatcagtat
    20041 tctaaaaaag ccattctaaa cgagagccgc ttaaagactt tacatagtct tttaaatact
    20101 atttttcatt atacccttgg tttctttttt atctatgcaa ttttatctgc tatcggtgtg
    20161 ccgatcggat cacttttggc tggtgccgga attgcaggtg tggcaatcgg tcttggtgcc
    20221 caagggttca tgagtgatgt tattaccgga ttttttatta tcatggaaca acagatggat
    20281 gttggggact acatcaagct agcaaactta acgattgaag gaaccgtcgt atctgttggt
    20341 atccgtaccc ttcagctgaa agcaacggat ggtactgtcc atttcatccc gaatcgaaac
    20401 atcaccacga tcagcaatct ctctcgtgca aatatgcaag tacttttaga tattcggatt
    20461 gttcctgaag aaggatatga caaaatctat gagatcattg atcgagtaaa tcgtacatta
    20521 gctgaaaaat accaagaaga gctccaaact gaaccttcta tctttggatt agtggatctt
    20581 ggaaacagca attttgcgat tcgtacggtc tgctacgcat tgaacggaaa acaatatgcc
    20641 ttaaaagaag agttcttgag ctcctatgtc aaagaattga cagcttctgg tttcacgatt
    20701 ccgaacagtc caattgctgt cggaaaataa tttggacaaa cgatgcaacg aatctcacta
    20761 aaaagagcgt gggacaaaag taaagaacac ttttgtccca cgctctaaac acgtataaac
    20821 ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
    20881 attttcgtga attccttctt atttcttgga gttaaacgct tttgtcccag cctctttttc
    20941 aagataaagt caaaactggt acgctgtttt ttctacttat tttttgatta tcttttagtt
    21001 tctcgttctt tagctcacga tatcgatttg ttcttttaat tcttcgattt tcggagacaa
    21061 tatattacga cgttctaatt catgcaccat gaagtttacg gctttaagca gctcttttct
    21121 tgtaatgatt cctttgaaaa tccgatcttc tgtgacaacc ggtaaaaaag gcgaatcgac
    21181 taacaaatgg agtacttctt ctagttccca attttcatca attgtcggaa catctgtttc
    21241 tgtcacatca gcaaccgtta gtccttctaa cgggtccatg ctgattgttt gcaagtcaat
    21301 cattttattg accacatctg ataaacttac tagtcctaca aagtgatcat cttttcctaa
    21361 tacaggaatt ttggaataac cgaccttcga taacacaagt aaaccatggg acaatggatg
    21421 atgatacata acatttgcca cattttctgc aggaatcaaa aacgtttcct gattttccag
    21481 cagcatttct cttactatag gtccaatcat ctgatggcct ccatttctat ttcattcaac
    21541 actactattt taccgaaacc ggatgcaaaa ttcataaaat aaagcttatt tttattaaaa
    21601 attagagttt ttttattcag cgctctctcg aaaaagaaaa ggccaactct tcaactgcct
    21661 gatgggcacg gttatagtat tgaacagaaa tttggtcatc taaactttcg ataagagcat
    21721 atgatgggat ttggatcggg cctctaggct gagaaatgct accaggattc aaaaacagga
    21781 ttttctgatc catctcacag cctatttgat gggtatgacc aaaaaacacc atatcggcag
    21841 ttgcagctcg agcttcgatt gctaactgtg tcaaaccaaa tcggacattc gataaatgac
    21901 cgtgagtcat gaatactgta tcctgaccag tttggatcac ttttttctgt tcgaatcctg
    21961 gtccataatc acaattacct tgaaccactt ggaatgtatc ccataatggg tcagaagctt
    22021 ctaattccga atcaccacaa tggaaaaaga tatccacttc agaacgataa cgatctacta
    22081 agtcaacgag gatatctctg tctccatgat tatcacttac tactaaatac cgcatctatc
    22141 tctctccttc cagccattgt ttccactcgg cactcaattt tttcattgct tgagcccgat
    22201 gactgatctt gtttttttct tcgctcgata attctgctgc tgtttgtttc ttaccttctg
    22261 ggataaataa aggatcatac ccaaagccat tttcgccaga tggtatacgg gctactcgac
    22321 cgggccattc cgcttctact acaaggctgt cttttttggg tgccgcgaat actaacgtac
    22381 aatggaattg tgctgttctt ttttcatccg gcacatctgt caattcatat aataatttgg
    22441 cgttattccc cgcatcactt ttttgttccc ctgcaaaacg tgcagaatag attcctggca
    22501 taccgcctaa agcatccact ttcaaacctg aatcatctgc taagactggc tgatcaagca
    22561 attgtgcaat cgtttctgct tttaatcggg cattttcttc aaatgtgctg ccagtttctt
    22621 ccacatctgg caattcagga tagtcaagta atgtttttac atgatatccc gcttctttga
    22681 acatatttcg aaactcttct gcttttcctg gatttcttgt cgcaatgatg attgtttttg
    22741 tttcttctat ccgctcatca ttttgagcaa actctgttag caaagcatgt ttttgctctt
    22801 tgatcaaatc ttcaatcgcg ttttttccaa aaaagagcat ttcattcaac tgttcaccat
    22861 caaacgttgc ttcttctcca gttccttgaa tctccacgaa ttttccagat tctgtcatta
    22921 caagattcat gtctacaagt gctgcggaat cttcttggta atccaaatct gtgacgcaag
    22981 tcccatctgg taagattcct acgctaactg cagcaagatg ttcttttatt ggatcttctg
    23041 acaattcttt ttctcttaaa agtttttcaa tggctaattt caatgccaca aatgccccag
    23101 tgatacttgc tgttctagta ccgccatccg cttggatcac atcgcagtct acgatgatac
    23161 tacgttctcc taatttttcc aagtctacta ctgcacgcaa ggaacgacca ataagacgct
    23221 ggatttccat tgttctacca gaaagtttcc ctttgctgct ttctctacga tttctcgtac
    23281 tcgttgctcg aggcagcata ctatattctg ctgtcaccca tcctgttcct gtttctctca
    23341 aaaaaggggg aacagaattt tctattgttg cagaacaaat aactgttgta tcaccaaaac
    23401 ggatcactac cgatccttct ggatatttga aaacatttgt ttgaagctcc agtttgcgaa
    23461 gctcttgggc ttttctcccg tcatgacgca tccttatttt cctcccaatt cgatatgttc
    23521 tacatttaag tgtccgattc caagccagtc ttcagctatt tcctcgaaaa gtttggcaga
    23581 gccagttgta taaaactggc ataatgtccg accattttgc ggtgaattgc tcaaattgaa
    23641 ataatctaac agcatagata cttcacctac tgtttctgct ccagaatcga tcagttgaac
    23701 attttctccc attacatttt gaatgatggg gcgtaataat ggataatggg tgcatcccaa
    23761 aatcaatgta tcgatttttt tagtggttaa aggagctaat gtttctgcca caattttttt
    23821 cgccaccgat gaatggtatt cattactttc gacaactgaa acaaatttgg gacaagcaag
    23881 acttgtcaca gtcaattctg gtactttctc tttcagtgct ttttcataag cttgactttt
    23941 taccgtacca atcgtaccga taatgccaac ttgtttattt tgtgtttttt ttactgccgc
    24001 tctagtaccg ggaaggatca caccgatgac tggaatagaa agagcagctt tgatttcttc
    24061 taaagccacc gcagttgcgg tattgcaggc gatcaccagc atcttgattc cttgctccac
    24121 cagataatcc gtcatttccc aagtatactg gattacctgt tccgcgggtc tagggccata
    24181 tgggcagcgt gctgtgtctc ctacaaataa aatattttca ttcggtaatt gtttcagggc
    24241 ttcttttact acagtcaagc cgccgacacc tgaatcaata aatccgatag ggcgattatc
    24301 tgtcaatcgt atcatccttt ttttcaataa attctatcct ccctattgtg tactttttag
    24361 cgtgaatttt caagtgctct tacttgctta aaacaatcga aatctctttt tttcgaatgg
    24421 aaagaggaaa agcggttgtg actttagttt gccacaaccg ctaaaagaat catctattaa
    24481 taactgtacc gtctcttttc caagtaaccg gaaagcagag aaagcgcgta acataccacg
    24541 aagtacataa gtgtcatgat gatgaacatc ggaagcacat aattggtgtt ctgtccataa
    24601 ataatacgtg cctgatgaga aagttcaggt aaagaaatga ttactgctaa tgaagtatct
    24661 ttgatcagtg aaatcaattg gctcacaatt gccgggatca ttgatttaaa tgcttgcggt
    24721 aacacgatcg ttcgcatcga ttctacataa gtcagtcccg ttgataatcc tgcttccatc
    24781 tgtccttttg gaacagcatt gagtccagct cggaaaattt ccgacaacat agctgactca
    24841 aaaatcgtta atgctgcgac agcggaccag aagatgttca tctgaatgcc gatttgaggt
    24901 aaggcaaagt aagtgaagaa aatgatcaat aataatggaa gattccgtat aatatcaatc
    24961 actacaccaa ggattttgga aaagaaaggg atattcgcaa aacgaatcac acctgtcaga
    25021 ccaccgatca agaaactaaa aatgatcgaa aacaatgaaa cttcaacagt aacctttaat
    25081 ccttccaata aaaaacggat attcatccat gaaaaagcgc cgctaaaatc catgtatttc
    25141 ccctccttct atgaacgaac cgcccatttt ttctcaagat aaaccattaa gtaagataac
    25201 ggtagcgtga taactaagta aaataaccca acgatgatat acgtatcgaa ggtattgaat
    25261 gtttcactag cgatcaagtc tccttgatac atcaaatcta aaccagctac gattgctaaa
    25321 atagatgagt tttttacgag gttgataaat tggttgccta atggcgggat aacgattttg
    25381 aatgcttgcg gtaaaacaat ataacgcatg atttctgaat aggtaaaacc agaagatagt
    25441 cctgcttctg tttgaccttt tggtacggtt tgaatccctg aacgtactgt ttcagcaata
    25501 aaagcagatg tatagatcgt taatccaatc gtacctgctt ggaatccatc tagattaaac
    25561 caataaatcg gtacgacaac ataaaaaaac atcacgatga ttaataatgg aatgtttcta
    25621 aaaaattcta catatgcctt tgctaaggcg ctcactactt tattctttga taattggaag
    25681 attgccatta acgttccgat aatcaaacta aacacaagtg ctaagacact cgaaaacaat
    25741 gtgatcttga acccgtcgac aaagattggt ccataagttt gtatcaattc gctcattgcc
    25801 tctccccctt actctacttt tccttcagtt tcatttggaa accatttttg atagatttta
    25861 tcgtatgtgc catctgcatg catttcctct agcgcttggt taacagcttt caagaagttt
    25921 tcttgccctt tgttgatcgc aatcccgtaa ggctcattag taaacgttcc accaactaac
    25981 tcatactctg gattttcatc tgcaatccct aacaaaatcg cattatccgt agtcatcgcg
    26041 tctccctgac cagattgtaa agcagtaaat gcttctgcat agttttctaa ttctaaaatt
    26101 tttgcatctg gtgcatgctg tcggatattc gcagccgatg tagaaccttt gactgctaga
    26161 accgttgtag aagcattcaa atcatctaca cttttgattt ggcttccttt ctttaccaac
    26221 aaagcttggc cagcatcgaa atagacatct gaaaagtcaa cctgtttttt tcgttcatct
    26281 gtgatcgtca tcgttgcaat aattgcatcg atatttccgt ttttcaataa tggaatccga
    26341 gttttagaag tgacttcaac aaattctgtt tttccattgt caccaaggat ctttttagtg
    26401 attgcttttg caatatctac atcaaatcct tgtacagttc tgctctcaat atccatcatt
    26461 ccaaataatc gagtatcata cttgacaccc cagatgattt cattggtaga tttgcttcgc
    26521 tctaaaatat cttcatttgc caacccattt cctttacatc cggctagtgc aagaaaagca
    26581 atgcctaaaa ggaaaatcag gcgtattttt tttaacctgc gcatcttttt cctcctatca
    26641 atgattgata actttactga taaattgctg tgctcggact tcatttggat tttcgaaaaa
    26701 gtctctcaca tttctgctgt cttccaatac ttctccatca gccatgaaga taacacgatc
    26761 cgccacttct ttggcaaagc ccatttcatg tgtcacgaca atcattgaca taccgtcatg
    26821 agccaatttt ttcataacgt ctaatacatc accaatcatt tccggatcca aagcagaagt
    26881 aggttcatca aacagcagca tctcaggatt catcgctaaa cctctggcga ttgccacccg
    26941 ttgctgttgt cccccggata acatggaagg ataagaatct ttcttatcta acatattgac
    27001 agttttcaaa aatttctccg cgtttttgac tgcagttgcc tggtcttgct tcaacacttt
    27061 gattggtgct aatttgatgt tttccagaac agttttattt gggtacaaat tgaaatgttg
    27121 gaaaaccatg ccgatgtttt ttcggacttc tgtcagtttt gtctttttat catgcaagtc
    27181 agtatcatta atcagcagct tacctgaaga gatcgtctct aatccattga tacagcgaag
    27241 catcgtgctc tttcctgaac cagaaggacc aatgacaacg acaacttctc ctttctcgaa
    27301 ttcgagattg atatttttta atgcgtggaa cttgccataa tatttatcca cgtgat
//
LOCUS       DACDHJ010000017        26506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDHJ010000017 DACDHJ010000000
VERSION     DACDHJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.017"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..938)
                     /locus_tag="IV223_000597"
     CDS             complement(<1..938)
                     /locus_tag="IV223_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8710905.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTK"
     gene            complement(1034..1227)
                     /locus_tag="IV223_000598"
                     /pseudo
     CDS             complement(1034..1227)
                     /locus_tag="IV223_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            1448..2662
                     /locus_tag="IV223_000599"
     CDS             1448..2662
                     /locus_tag="IV223_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8710906.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2684..4699
                     /locus_tag="IV223_000600"
     CDS             2684..4699
                     /locus_tag="IV223_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8710907.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4782..6155)
                     /locus_tag="IV223_000601"
     CDS             complement(4782..6155)
                     /locus_tag="IV223_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8710908.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6181..6465)
                     /locus_tag="IV223_000602"
     CDS             complement(6181..6465)
                     /locus_tag="IV223_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8710909.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6467..8482)
                     /locus_tag="IV223_000603"
     CDS             complement(6467..8482)
                     /locus_tag="IV223_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8710910.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8661..8966)
                     /locus_tag="IV223_000604"
     CDS             complement(8661..8966)
                     /locus_tag="IV223_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8710911.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(9055..10086)
                     /locus_tag="IV223_000605"
     CDS             complement(9055..10086)
                     /locus_tag="IV223_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8710912.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10293..11168
                     /locus_tag="IV223_000606"
     CDS             10293..11168
                     /locus_tag="IV223_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8710913.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11320..12162)
                     /locus_tag="IV223_000607"
     CDS             complement(11320..12162)
                     /locus_tag="IV223_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8710914.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12254..14131)
                     /locus_tag="IV223_000608"
     CDS             complement(12254..14131)
                     /locus_tag="IV223_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8710915.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(14128..15549)
                     /locus_tag="IV223_000609"
     CDS             complement(14128..15549)
                     /locus_tag="IV223_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8710916.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15552..18941)
                     /locus_tag="IV223_000610"
     CDS             complement(15552..18941)
                     /locus_tag="IV223_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8710917.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19198..20589
                     /locus_tag="IV223_000611"
     CDS             19198..20589
                     /locus_tag="IV223_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8710918.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20662..21309)
                     /locus_tag="IV223_000612"
     CDS             complement(20662..21309)
                     /locus_tag="IV223_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8710919.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21317..22003)
                     /locus_tag="IV223_000613"
     CDS             complement(21317..22003)
                     /locus_tag="IV223_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710920.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22009..23082)
                     /locus_tag="IV223_000614"
     CDS             complement(22009..23082)
                     /locus_tag="IV223_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8710921.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23304..23978
                     /locus_tag="IV223_000615"
     CDS             23304..23978
                     /locus_tag="IV223_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8710922.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23983..25323
                     /locus_tag="IV223_000616"
     CDS             23983..25323
                     /locus_tag="IV223_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8710923.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25472..26458)
                     /locus_tag="IV223_000617"
     CDS             complement(25472..26458)
                     /locus_tag="IV223_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8710924.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7654 a         5009 c         4891 g         8952 t
ORIGIN      
        1 tcttttgtga cgtaactcaa aaatacttcc attggtgtcc gatagtgtaa tgattttcta
       61 ggaatatgat ttcgtttcga tgccaccgat tggataaagg tttcagatac accattaaaa
      121 tccatagctt tatacaaacc atctttacgt aataagccat ttgaatgttc attcaatcct
      181 cgttgagaag gtgttcctgg atccgcaaaa taaattgaaa tatcgttggt attactaatc
      241 tttttccaat ttgagaattc ttttccacaa tcaaatgtga tggatttaaa taagtttttt
      301 gggacacaac gaaaccattg attcagccta ttttcaatgt ctttagcctg tctgccttcg
      361 ggtttcaagg taatgatgac tttagatagc cgttcaacta atgtgatgac cgcgctttta
      421 tgttttccac ccacgatagt gtcaccttcc aggtgaccaa attcatgatc aaactgggta
      481 tagtctgtct ttctgtctcg gattgaccgg cgaaaccctt gttttcctct tttttcttta
      541 tgaccgtttg gttttctttt tcccttcatt ggtaaatcgt gagtgtcaaa ggttttatct
      601 ttaaacattc gatacagtgt gcgtaccgaa caagcaatag gaaactctgc tcgaccaata
      661 aggacatcag gagcccaccc ttgaacaacc tttttttgaa tgtattcctg ttgctcatca
      721 ggtaaaacaa tcggatgtct tccgcagtta cgtttgtttt tcttatattg tttatagtag
      781 ctcattgcgc ttccaccaga tttaagaaaa cgatagacct tataaatagt ttgtcgagaa
      841 cgttgtaaca gattggctac ttttgaaaca ggttgtgatt gattgaaata agcttctatc
      901 attacgagtt cagttggtgt aagatggtta taggtcattt gtactcactc cttataattc
      961 tttcgtcgga actattttga gtgtagcaca aatgactttt tttagttgtc tagcttaatt
     1021 ttacaatcgg cgagatgttg attaaaatat cctcttgccc tgtgtattcg attctttgag
     1081 gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga tttttggcgg
     1141 tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt cacgctgcag
     1201 agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt tccaattgac
     1261 tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact gctattatat
     1321 gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac ttcctgtgat
     1381 tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt acaaaaatga
     1441 aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag tctctcttat
     1501 tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta cttatgccac
     1561 gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg tcactatttt
     1621 tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg tgatcatcgg
     1681 attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt acagtattgt
     1741 cttagcttca agagctggac taggtattgg ctttggattg atcaattctt tggcagtcag
     1801 catgattgct gccttttatc acggagatga acgagcctct atgattggtt ttcaaagtgc
     1861 atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag tacagttcgg
     1921 ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct tgtttgtttt
     1981 gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc caacaagaca
     2041 agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc tggtcatcgt
     2101 ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt ctctaggaaa
     2161 cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg ctgccggagt
     2221 ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt ctttactact
     2281 gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga ttgggtttgg
     2341 tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca accaagtaag
     2401 tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg ttggcgctaa
     2461 tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt ctcttgggag
     2521 taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg cgcttattag
     2581 ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg aagaaataaa
     2641 agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa atgaaaaaca
     2701 taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac tgctacaaaa
     2761 tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg atgattgcca
     2821 tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt ttaaagtgac
     2881 gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg gaaaatggct
     2941 gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat cagggctacc
     3001 agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag caagtaattt
     3061 tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa aagatccaaa
     3121 tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga ttggcggagg
     3181 aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat ctctggatga
     3241 ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta tggatatcga
     3301 ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta aacgcgcttt
     3361 tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag tttttcatga
     3421 atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac tgaatcagat
     3481 ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta tcgacggtat
     3541 caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga tcaatcaata
     3601 tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag gacaaaatga
     3661 aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga ttggtatcga
     3721 aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc ctaagatgca
     3781 atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt atgggagagc
     3841 aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc ttgctgcaga
     3901 acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata tcttagctga
     3961 aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat atcaactatt
     4021 gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta tcgattgcta
     4081 tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt tattcgattt
     4141 tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg acactatcac
     4201 acagattact actccatatt tcaaattcta tgaacaagat gctttatgca aactcggaca
     4261 gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat ctcatgatgc
     4321 ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt acagtatgta
     4381 tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa tctatctttt
     4441 aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat ttatgataca
     4501 acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag atggaaagat
     4561 ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag gcggaacagt
     4621 gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct taacaagcta
     4681 ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga ctttaaatct
     4741 tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg ttctttagaa
     4801 acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag taaaaagaaa
     4861 gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc aagtacaagt
     4921 cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag atctcctaat
     4981 actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc agaacctaat
     5041 accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc tccgcagaag
     5101 aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat agcaaacatg
     5161 gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt aggtgcatat
     5221 ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt tttagcaatc
     5281 ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag gacgataaca
     5341 aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt gtctccattg
     5401 cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac atatacgatc
     5461 gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact tttaggaatc
     5521 tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca tccactagaa
     5581 gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt gatgttcttc
     5641 ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa gaccccgcct
     5701 aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac tgctgttaga
     5761 caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct tgtgaatcta
     5821 gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg tgtaccgtat
     5881 tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg agtatgcaga
     5941 gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc agcaccgccg
     6001 atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc tgaaaatttc
     6061 ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga gggctgactg
     6121 acgattgaca caaacaaatt caacaattta tccattgata gttcctccct tgaatcattt
     6181 ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt caatgatcga
     6241 ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca aagaatgctc
     6301 aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat gacttacttc
     6361 aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca tctctaccat
     6421 aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat tcgtctcctt
     6481 ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct tgaagatttt
     6541 gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc tcattatcga
     6601 tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc agatccacag
     6661 gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct ggacgagcat
     6721 gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc acgcgctcga
     6781 tcatagcttt gatataactt tcttctattt tattttctga taatagcggc tgtgcggcaa
     6841 atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt tcttctgtta
     6901 ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg ttcattttac
     6961 gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg atataaggca
     7021 gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc agccaatctc
     7081 tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga tgcagaacat
     7141 agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata atatcgtaag
     7201 aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa aagatcatcg
     7261 ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca cttggacaga
     7321 cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg atctctccgc
     7381 caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct attcgtcttt
     7441 ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga tgcttaattt
     7501 gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag gcagggacca
     7561 agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg gccatcagga
     7621 attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt gcaggctcct
     7681 ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc tccgaatttt
     7741 gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg actgcaagag
     7801 aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt cgaatggttg
     7861 tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga agatctgata
     7921 gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg gaaagggttg
     7981 cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga gcagtgcgat
     8041 tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca gttttcctag
     8101 tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg atatctgaca
     8161 ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa acagacgttg
     8221 cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta tacacagctt
     8281 caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat ttctttatac
     8341 ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc gcctcgcttg
     8401 tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt gttttcagga
     8461 gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct agtatggtag
     8521 cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt acgactgtac
     8581 cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt ctcctgcttt
     8641 tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt cagttcttca
     8701 aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg attgggctca
     8761 ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc tttattttct
     8821 gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc accagaatta
     8881 gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct taatcgtttg
     8941 tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat actttttcat
     9001 cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa tatgtcatat
     9061 ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca tttgtaagat
     9121 tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt aaatcccttc
     9181 gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat aactcccttg
     9241 ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa agtcttcatg
     9301 atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt gtttcacgaa
     9361 catccctttt tttccgtaaa cgacgaaact tggacgttct ttgatgcgga aataactgga
     9421 ttttatcgaa attttcatat ttccatagaa aaaatccaga tcgaagtaat cattcattcg
     9481 tcctttccct aaaagttgac gtacatcata gctgatatca tctggttcgc cgaaataaga
     9541 aagaacttga tctactgtat gacaagcatg tccatataaa tagctgttcg cagtagaaaa
     9601 ttcatgcgta ttttctggta cttccggtct gaaataatca taatgcattt ctacttctaa
     9661 aatatcccct aattttccgc tttcgattac tttttggact gtcaagaagt cagaatcgaa
     9721 ccgtctgttt tggtaacatt ggataaatag acctttttct tttgctaatg caaataactc
     9781 ttctgcttcc tttgacgttt ctgcaaaagg tttctctact agtacattct taccatgttc
     9841 gagtgccatt tttccatatt cataatgaaa ttgactaggt gtagtgatga caactaattg
     9901 gatctcagga tcatcccaaa gatcagacag ctgatcagta tattcgatgt cttcgaatgt
     9961 tttccacact ggttcagatg gggaaaaaat ttttttgata ttgattttgt tcgtaagttt
    10021 tcttgaaaaa ggcaaatgat agcgatttgt actctttcca ttgccaagat aagcgattgt
    10081 aagcatgagt ggtgcctcct tttattgatg ttttcagtat atcctagaaa agcgcagaaa
    10141 gtatttataa tagctattct ggtaaaaaag ttcagaaaaa cacttgttat cattcggttt
    10201 ggtgaaatta atgttcaaag taaggataaa aatcaaaaag atagtagcgg aaccaagtgt
    10261 ttatcagtaa gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa ctaacaaaac
    10321 aagagttgtt taccacgaca gagaaacgga ttgtcgatta catccgtagg aatatcgaag
    10381 ctgcagtcta catgacgatt gaagaattag caaaagctac gtatacttcc cattcagcta
    10441 tcattcgttt atgcaaaaaa atgggattca gcggatttaa agaattccgt ttcgagctgg
    10501 ggcgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct aattttcctt
    10561 tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta tcgatccagt
    10621 caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc ggtaaagtat
    10681 tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc acggcaagga
    10741 aattccagaa taaaatggtc aagttgaata agtttttgat catggctgaa gaatacagtg
    10801 attcttcttg gaatgcagcc aatttgacta gcgaggattg cgccattttt attagctaca
    10861 gcggtcgcat ccatcattat gaacgaataa tgacctattt gaagcatgta gaggccccta
    10921 ccttgctgat taccggaaat cagcattcag aaatggccaa acaggctgat atgtgtcttg
    10981 ttatatcgca ggaggaatat gattttgcta aagtagcgac tttctcctct caaattgcct
    11041 tcgattacgt attaaatacg ttattctcag tgatatattc tcaaaatttt gaagagaata
    11101 tccgcaactt gaaaaacaaa caagaattga tgcaaaaggg ccttcttaaa gaatatcgga
    11161 aaacttaaga aaagatcctc atttagtaat aagaaagata gtgtttgata ggctctcttt
    11221 gatatcatca taaaaacagg ctgaagtttt ctttttcaaa cctcagcctg tttgtttacg
    11281 gaacgattcg aatagatata ttatagaaat gaggaatagc taacttctgt ttcttatttt
    11341 gaacgattga cgaaaaagat atataagaag gaggaaaatc agtatacatg cagctaacaa
    11401 tccatacagg aaaaatcgtt tgttctcgga tactttctgt acttcttttt gaacttcttt
    11461 tgcatgataa ggaacccggt gccctctgac aagtaagcga tgactgttga ccatataagg
    11521 tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg attctcagat cttttgtatc
    11581 agtcggttct accgtcttga tctgatcgac ttgataagcg agtgtccgtc cattgatttc
    11641 gatatagaaa gaatcaccta aagtcagttt cggtaagtct gtgaacaact ttgcttgagg
    11701 cagtcctcga tggctggaaa taacggcatg tgtattttct ccccccgtag gataagacgt
    11761 tccttccaat aatgaggcac ctttttctag cagcacagta ttcgtttcat caaaaattgg
    11821 caaatggaca ttgatctcag ggatcgtcaa aataccgatc gtatgtttct caaaataggt
    11881 tctatccttt ttcgcaggtt cttttttctt agtaaaggga tctgcacctg gatttccacc
    11941 ttcttttgct aattgctggt ttttctttgt catgttttct tggattttag ccatgacttc
    12001 ttcattttct ttgaccgctt gttgctgata atgagagatg atctgctgat ccagataatt
    12061 attcagcgcg tcgctaacaa atggataagc tagtatccct attcctgaga gaagcaagaa
    12121 aagcatcagc agatctagaa gtttcctttt ctttggtttc atttgtgttc tccttgtgat
    12181 gactgtttga taataaaaga aaaaggagca tttgcgtaat ccgctgctcc tttttccaat
    12241 tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc aaagtacagt ccagcaatca
    12301 gtagaaggac tactcctgca ccgatataca catagattcc cttaccgcct gttgaaggaa
    12361 gtgtaccttt gtgtttattt ggtatttttt caggagaaat caactgtcct gctgttacat
    12421 aagattgttc atcgatagta aatgctacac ggtttgttaa tggaacatat ttttctggcg
    12481 ctttcgtttc ttccagatag tacgtgccat attttagacc tgtcacatcg attaaaccgt
    12541 tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga tacccagctg acggcttttg
    12601 tggatgggtc gatcgataaa tatttcgctg tgtcactatc ttgatcacga acgacgaatt
    12661 ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc tactttaaca aatcgtttgc
    12721 ccccagtaac gacatcgact gacggtggtg tttgatcatt tgtatgcccg ttatcgacat
    12781 ttgcttggtt agaaaatcct ttggttggat ctgctttttc gttcaaatgc atatagtaaa
    12841 cgaatttcaa tgtaccgcca ggagttaatg aaggaatata attcggatca actgaaaccg
    12901 tgaatccgtc tgtttgctca gtgacagaat aatttactgg gtcgatttct ttatttccat
    12961 catataaggc ataagcatac gttcctgaag aatcattatc aaatgttaaa gcagcgtcat
    13021 gagtatcgat aagtttgaat gttgtgtact tgttttgcgt tccttcttta tcagcgatac
    13081 ctaatgggat attgacagaa atctcatatt gaattttttc accgattgcg acatcttttc
    13141 cattcaattg aggtgttgtt ttatcaactt tagacgtatc atttttgatg gtcttttcta
    13201 ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat tgtttgcttt tcaatcattt
    13261 ccgcattatc aggagctttt acttcttcta aattatattt tccaacttct aaatgattaa
    13321 cgatcaactg gcctttttca atagatgcct cggcaaagtc attgttgccg atgtcataag
    13381 aatgaccagt aatgaaatgt ttggctttgg tttgatcagt tgtccaagtg tacaatccat
    13441 ctgtgacact ttggatgtat tttttgacgc ttggtgttcc ttcttcttta gaaataataa
    13501 attctgctcc atttagtgct tcgttttcgg cagtaccgat ttttgtaact ttcaacgttc
    13561 catcattacc gactgtattt ttagggtaga gatggatagt atctagttct tctgtcccgt
    13621 atttataaga gccatctgct tgtttgatca tctcatatac agggaaagct aaaaccatgt
    13681 ttgcggcagc tgacactccg tcttttggtt cttctttgat cgtgtagact gcatctttcc
    13741 cattttgttt tttaggtaaa gataaagtga catttccatc agcatctgtc gttcctgaag
    13801 caactggtgt accaggagtc aaagactgga ctgcttgttt tgctgcatcc acggacgctc
    13861 ctttatctcg ttgcgcataa aattcttgag tgacgttata aactgaaaat gaaatatcgg
    13921 ctaatccttg gtattgatcg aattcgctca tttctttccc gctgttttgg attaaaggat
    13981 cgggtaaatc agtcattttc tttttatgaa ggatgacttg agctgctgtc tcctctgcaa
    14041 acactttttt tgcgccgaag ctgtttgtga gtaatggtaa aataaggaaa aggactccta
    14101 acataacgtt tattttttta tgatttttca ttttttcact cctgttcttt ttttgataaa
    14161 caaccagatt gcaaagcttg cgaataccat accaacaacg cctaaaagtg tatttttcgt
    14221 ttcgtttgtt tttggaagaa atggacggct attttctttt ttattgacta ctttcaatat
    14281 actgtccgcc tctttatcct cagaaagaaa agttgaaatg gtaaacggaa tttctgtttc
    14341 acttaaaata taaccagaag gtgcttcgat ctcttttaat cgataacttc ctgttttcaa
    14401 cccggaaatt gaaaaagcgc catttttatc ggaaatcagg tgaagcgcat ctgattcgtt
    14461 ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa tttcctgctt tgtttttaat
    14521 aaggaatact gcgccagcta atcgtttatc tggtttattt gtctcgattt tgacaaattg
    14581 cttactccct gtatatacag agactttctt tgtactgaca ccaaaaccaa aatccagtgt
    14641 tccgttgttg ttgatttcct tgttcgcctg tgctgtactg cttagctgca tctgataaga
    14701 aatggtcagt ttttttccag catatttttc caacgaaggg atcgaaaata aaacatcaaa
    14761 tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc ttttctccat tcgatgaaat
    14821 cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca ctatctgaca attcgaactt
    14881 ttgatagtca aggatattta caggaattgt cgtatgcaag gaataagtga ttttttcacc
    14941 aatcgtaaaa tcatttctag gctcttctaa taccttctca aaaggtggat cagggtagtc
    15001 gttttcttca tttttaggat acaaatggat agttgaaagt ttttgattgt tttgtccatg
    15061 tacaggaaga acaactacca tattctctgc cttttctttg acgacttctg gtactttgga
    15121 ttcaatgaaa agatacgctt tatctctttt ttcttgatct ttagcggcca gagaaaaaga
    15181 agcgatacca tcttcgttgt taagagttgt agttgttgct tctgcggtaa acataccgga
    15241 agacgcaccg ttttttgcga tctctgcttg tgcttcttct accgttttgc ccttttcccg
    15301 tagatggtaa aaagaatctg tgacatcata aacttggaat gtgacaccat ttaatcctcg
    15361 atacgtttgt aataaagctt tttcttgtcc gtcatttgga tgatttttcg gcagttgccc
    15421 gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt tctgtttctg tttctgttgc
    15481 tacctgagta aaagctggtt taaatagtaa caagaagaga cacatactaa gccaaccaag
    15541 ttttttcata tttagctgcc ccctctttct tttttcaaaa aaagatacca aatagaaaac
    15601 gcacaaccaa tcatccctac tagatagatt cctaaacggc caattcctcc cgtttcaggt
    15661 aatggtactt ttgcctgatt cgtgatgtct aaagaaatct ggttgttttt ggctcctggt
    15721 gacagaacag attcatgatc ttgtccatcc acttgaattg acccatcttc gtgtataact
    15781 atagtaactg gctcttttag accttggtat ccttctggtg taaaagtttc ggtcagcgta
    15841 taagttccag gttttaaatt ttcgaataga aaggtatctg tttcttgtcc atcttttggc
    15901 gattctaagt ccatttctgg tccctgcagt ctgaattttg ctccttttaa tgtctgtccg
    15961 ttgtcttctt ttttgtgtac cgttagatca aacgctttta gtcgattttg atgagtgagc
    16021 gtccatacat tatttgtcat ctcggtattt tttcccgtgt aaatgatttt tccatattta
    16081 tcgatttgga attcataatt tcccggcgaa gtatcatatc cggcaggacc agctgtttca
    16141 acgactcgat atttaccagg tgtgagtgaa tcaaaactgg caagaccttt ttcattcgtt
    16201 gtttggctgt caattggctg ataagtacca tttgcttctt ttttctgtag ggaaaatgct
    16261 gctcctttaa gatttatctc tttgtccgtc ccttgcatcg catacttacg tactgctaca
    16321 ggaacttcaa caaaaggatt ttcaatagtc aactggatcg tatcatcgga agtagtgact
    16381 gtttttccat caatggttac cgtaccatca gaagcgatct tgatttccca agtagtcttt
    16441 ttgcttaatc catgcccttc tggagctttt gtttctgtca gcgtataggt catttctttt
    16501 tgcaatttga cattttctgg aagagaatag gttccgtcgc catgatctgt tagtaaagta
    16561 tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa gatccttttc acctgtagag
    16621 gaatttttcg ttattttcaa gtttaacggt gtgaattgtt tagtattttt ccatgtcatt
    16681 gcatctattt gttgagaatc gtatcctggt acaggtaatt ctttgattgt ttggtaactg
    16741 aatgcttgac cttgattatt gtattgaggt agtgataata tctcttgata actttctccg
    16801 ctatttgcag ataatttgtc aatgtctgca cgttcccacg tatttgttgt atcttttgct
    16861 ggtttaatga ttcgaatata tccgtttttc caagctgcag catttgtcgt atgttcccgt
    16921 tgaatctcaa aagtaacttg atctggacga tcagctagat tgttgtcaaa ttcttcccat
    16981 aactttttga tgtgaagact gactccggga gctttagcag atggtatacc gaattcagct
    17041 aattcattgg tatcaatgtt tggctggaaa gttgtcctgc cgttcatttg ataccagaaa
    17101 tttggatgga agtcctcatt ttctgtttgg attctcactt ggtaatggat ttggatttct
    17161 tggtcttttc ctaaatagat ctgattgctc ttgatggtat ttccttctat ggaaatagat
    17221 ggagtgaccg ttgtaggact tgtccccaca ctcttgactg aaagtgtacc aggctgataa
    17281 atgaatggtt ctgcgattgg atcatttatt tgtccattgc ttacagttgc tgagatctgt
    17341 acagcttttt tggctaggta ttcagagata tctgttgcat gatcagctga ttcatagtaa
    17401 agatcgcctt tttcatctga tgaaaccatt tgtcgcatac gagactctac ttctgctttt
    17461 gagagaccag ctgccggatc gctttgaagt tggatgccaa gaccatgtat ttcgattcct
    17521 cgttctttga gtgccatcgc ttctccgatg gttgcgataa acgtactgtc gattcttctg
    17581 gatagattgt tttggtcagg tgcatcatag attcttgaga ttagagaagt atttcccggc
    17641 cgatcttgcg tattagaaaa ctgagttccg taataattgc tgcttgattg tgcgtgtacc
    17701 cgctgtactt tataggaaaa tgttggtaca ccatccgtca gcaaaacgat cactttttta
    17761 tgtccatttg gaacggatag catgcttcct gcatctctta gtgctttttg tgtaaaagta
    17821 ccaccatttg tccgtgaagg tgtaatggat tttacttgat ttttcactga atcaaatgaa
    17881 cccatctgta ctgcaccgtt actgtagcta tatccttcgc ttgagtagcc gacatatccc
    17941 atattgattt tgtctgtgat accgctatct gctaaagtat cgacaaaacg atcgacacca
    18001 atctttactt caccgatccg attattgtcg ttcatacttc ctgaccagtc tacgaccaat
    18061 acgagatcaa gaggcgtgat atctttttgt acatttccac gagcattcaa ataaacatca
    18121 aacaatccgg gtgtacttgt ttcctttgca tactttcgta tcgcatagtc tgcttgatta
    18181 ccggttcctc catattcgat atacgaatgg gtccgatcat taggatctcc attccaagaa
    18241 tttataccat cccattgatt tgttccgttt ttattgcctt gatgatcaag aacatttgta
    18301 tttcctgtag gttgccaact ggctttagga taagtgccct gatcatctgt tgtatattct
    18361 gtaggaatcg tttgattaga tggcacactc acagctggca aggataatgt aggcaatgta
    18421 aatgggctga cagaattttc gttttctttt gatgattctt cttctatttc gtctggcttt
    18481 ggtactgaca catctgtatc tttcttattt tctgtctcat caataatgtc atattcttta
    18541 ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg ctggatcgat tggaaaactc
    18601 agatcgatag tttgtttagt gtttgatgag atttccaacg taattttccc gctatttacc
    18661 tgataagcat agccatctcc ttcttttctt tccacatcac ttaagtaatt ttcgactaga
    18721 ccaatttctt tttctatctt ttcagcactg gtgttattcg catttagctt gatgttcagt
    18781 gctttttttc cagactgatc cgaaatgact tcagctgaaa ttgtttctaa aactgttgga
    18841 tcatctgtag tgtctgctat tgcagataat ggcacaaagt ttgataaact caatattatc
    18901 agcatgaaaa taactttcag tttcttccct gtggtcgtca tttttcccct cctttttctt
    18961 tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata aacaggcgtt acataatttt
    19021 atttatttta atatgataat tattatacga agggcgctaa agggaatcac accaattttc
    19081 ataccaattg gtttcattta tgaagttgat tccttcgatt aatgagcgtt tcattgaaag
    19141 aaaagggttg agggagaaaa acatatgatc aggttgttgg aatgaaacta tagaaagatg
    19201 gaaaaaatca ttcgaattct gtccatttct gacaagtgga tgactaaaaa agaacttgcc
    19261 aaacgaattg gtagttcaga atctacattt gtaaggtata tcgaagagat caaaatccgc
    19321 tggggaacgg atgtaacgat tcaatcttca caaaaattgg ggtatcgtct tgaacatttg
    19381 aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt
    19441 ttattaaatg agattattgt caatccgggt aaaacgattg agtattattg tgaaaaaatt
    19501 tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc
    19561 tatttattaa aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa aagtgagctc
    19621 cagttgcgta tctttgttac ctttttcttt ttaagttact acggaagacg cacgttgcca
    19681 tttccaatcg aaaaaaagaa gatcaaagaa attatggtca agaataattg tcagatgaat
    19741 ttgtttcagg aagaaagccc atatgagcaa tctttcttta ttatgtattt tgttgtgcat
    19801 ctcatgagag aatcccaagg aactccattt gaatggaaag ctggagactc ttatgtacag
    19861 ttgataaata tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag
    19921 tacagcagct atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg
    19981 gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa atatgtccta ctgttcgttt
    20041 tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt
    20101 tattttttct cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac
    20161 tttgcacagc agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt
    20221 caattttatt ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt
    20281 tggatgctta tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt
    20341 atcagcgact taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt
    20401 gctgtgcata aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac
    20461 aatcttctcg agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct
    20521 catatattga ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc
    20581 gagttatgaa taatcaaaaa acagctatgt ataaaccacc ctgataaaaa tttcaggaca
    20641 aatacacata gctgtttctt tttattgtat tccttcagca atccaacgag atttatagcg
    20701 aaagtctttt aacaactctt cttttgtcca attatttgca atgagatccg tcaaagtgtt
    20761 cattacgagg gtcatggaaa agtataggaa actaactgct tcttccgacg attgaatgga
    20821 gaaatgattg attcttaata attccagcca tttttcgttc attggagaat cagccgatag
    20881 ttctctacgt ttggttgtct gctgatcatt tcgctgggtt aacaagtata acctttgcca
    20941 atagatactt tcgtgatcta agtcgcagca ccactctaaa tatttttcga tccccaaaaa
    21001 gaaatgttgt ttgttttcgc taatgaattt taagatatct tgatggacta tgtgagaact
    21061 gcttttgatc gtatacaaat aagcatcttc caaatcttga aaatatttat aaaaagcacc
    21121 gcgcgacatc cccatcttgt caacgatttc tgagactttg acttggctga tgtgctgtga
    21181 gcaaaatatc tccagtaata ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa
    21241 aaaagtttct tttggcatcc acatctctcc tttatctctg attatacgtg gatagccctt
    21301 cattgtcatg aaggattcat tccttctttt tctcttcttc tgtcaagtac ccatcttcca
    21361 ttcgataaac gcgatcactc cattgaatca tacggacatc atgagtaacc ataatagtcg
    21421 ctttttgttt ctcatgagct tctttaacta gtaatttgac gacttcatat gcatgatctg
    21481 tatccaaact agctgttggt tcgtctgcta agatcaagct tggctcatta tataaagcgc
    21541 gagcaattgc cactctctgt ctttctccac cagaaaggtc tttaggataa ctatttttaa
    21601 gtgactggat atctaaagat tgcagcaagg aatcgatttt tgttgaatct tctttttttg
    21661 aagtaacttg gtcaactagt tcaaattgtt cctttaccgt taaaaaagga atcaaattag
    21721 agctttggag aataaatcct atttctttaa agcgtaaatc tgctcgtttt ttttctggaa
    21781 gctcagttag tgagtgaccg ttgatcacta tttctccgga agtcggtgtt tgcaatcctc
    21841 ctgaaatggt aaggaaagta cttttacctg aaccagaagg accaatgatg ctgacgaatt
    21901 ctccttcctt tacataaaaa tcaattgctt tcaaagctga gacctttgta tgaccagaac
    21961 caaatgtttt ggtaactttt ttcatcgtta atattttttt catttttatt aacctcctat
    22021 tgctgtaatt gggtctactt tggttatcgt acgtatcgaa aacaatccgc ctagcattgc
    22081 gacaatgatc aaaatcccac tatatagcaa ccattgtgac caaaaaacag caaaaggcat
    22141 cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa agagcaagca gaacgcctac
    22201 caccccaaca ataaaggact gtgctactaa agatttagcg atgaagaaat ttttgatccc
    22261 ttgtgctttc attactccaa aaatagcagt cttctgaagt gtcatcacgt acatgaaaat
    22321 accgaccacg gcagcaacca ctaagaacaa aaagtatatc atcgcattta aggtcatatt
    22381 ttgggcagaa tagccaggaa tactttcaat gaatgtccca atagttaata actgtagacc
    22441 cttcgatgct ttttctttcg aaattttagc tgaatccttt attacgatcc cattgatcgg
    22501 ctgttcttct tcagaagcaa acggctgttg tccatacttc aaagcagtcc acgtatctag
    22561 ggacgcatag atgacgggag aaactgtgta ataagtttca ggaaagatac cgacaatcgt
    22621 caaaggatcg tcgtaactgc caattttgat cgtatctcca attttatagc ctgaatcagc
    22681 cagattttga gagatgatga tttcattctt tgttttaaat tcatgccctt tcgtgagttt
    22741 tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta acattttctt tgttttttcc
    22801 tttgattgct ccgctgtaca atccgattgg tgctttctct cctccttcaa tgtatttcag
    22861 atctcctctt gttaattgtg aagctgcaaa tacttgattt gcatcctccg acaaaacaat
    22921 ctcttgtgcg tcccaatcat cgattgcttt tttaaactct tctgaaagtc cattcgctag
    22981 tcctgacaac atgaatacga catacgcaat caagaacata ataccgacga tcaagccata
    23041 tcgtaattta gaatagcgaa tttctttgat agctagaaac atttgttttt cctccctatg
    23101 atagattgat agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag tgacaatttg
    23161 tcacttgtgt tgtttttttc ttatcaataa attgtctaag acatctttcg ataaaacctt
    23221 aacatgagtc ataaaaaatt tatttaagcc ttatttatgc tttccatgat aaactaaagt
    23281 gaccaaatca atgaggtggg aaaatgatta aactattagt agtagaagac gataaagtat
    23341 tatccaataa tataaaagaa atactcgctg caatcggcga gataacacaa gtttatgatg
    23401 gtagtgaagg actgttcgag ttggaaagtg gagtttatga tcttgcaatc ttggatttga
    23461 tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga
    23521 ctccagtatt gattttgact gcaaaagata gtttggaaga taagattgaa ggatttgaac
    23581 gaggtgcaga tgattatcta acaaaacctt tctatcgaga agaactgatc atgcgcgtga
    23641 aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag tataaggata
    23701 tcagttgtga tttaaaaagc aatgaagtct tgtataaaga acaagttttg cccatacaag
    23761 gaaaagagtt tgaattgctc gtttattttt tacaaaataa gggaatcatt ttgacaaaag
    23821 agcagatatt tgaccggatc tggggatttg attctgatac gacgattaca gttattgaag
    23881 tttatatgag caacttacga aaacatctgc gaagtactgg attggataaa gaaatcaaaa
    23941 ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg caatgaatgg agatattaca
    24001 cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag catttgcttt tatctttttt
    24061 cttttgggat tcattacttt acagatatta aaccaatctg catacagaga aacagatctg
    24121 tcattagaga aaataaattt tgattctcga acagtacaat tagagataca aagatatcag
    24181 caaggcgatc cctttttaaa cccgactcaa acggaacaaa acagaacaaa catggaacag
    24241 ccattaatgg aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat
    24301 ggtgagatat tgaataaagc agctattggt gggcgtttca accaaatcaa agaattaaaa
    24361 atggatacga aaaatctgaa taccatccaa aatattgaat taaatgaaga cagtggctca
    24421 tctgccctct ctttccgttc gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc
    24481 tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt cattagcgac ttttcgcacg
    24541 attatgattt tgtgtatgat tgttttttgg attctctcta ttggtatcag ttattacttg
    24601 tctagtctaa gcatgcgccc tattattctt tcttggagaa gacaaaaaga atttgttgag
    24661 aatgcttctc atgaattgcg cacaccgtta acgattattc aaaatagttt ggaacatctt
    24721 tttaccaagc ctaatcatac tattattgaa gaatccgaaa gcatcgctca agcattgtca
    24781 gagacgaggc gcctcactgg attgacttcg gatttgctga caatcgctcg gaatgattct
    24841 gatcagcagc tgctttcaaa gcaaatgatc aatacacaag aatatatcga acaacttgtt
    24901 aaaccatttc aagagatggc catcatagat ggaaaagagt ttattctcca taacgatgca
    24961 acattagaag taccaattga tgaaaaaaaa atccatcaag ttttggttat tttgctggat
    25021 aatgcattaa aatatacgaa tccagaagat aagatcattt tggattcatc tgttaaccat
    25081 aaagaatggc tgattcacgt aaagaatact ggcccttcta tcagtgaaga agatcaagca
    25141 aggatttttg atcgatttta tcgggaagat caatctcggg cgaaagagac tggcggatac
    25201 gggcttggct tggcaattgc aaaacaggta atcgaacagc atcatggaca gatttctgta
    25261 tccaattatc ttccacaagg agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa
    25321 taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct tttttgttgc
    25381 gaataaacgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc tttaagctga
    25441 ccacttctgt cacaacctct ttagcttacg cttacgattt tttattgcgg tcttcaatgt
    25501 gtacatcacc gcttgtgtca atatccaatt cttctcggct aacatcttct gtcacttttt
    25561 caacatcttc atgttcttct ttatgaatcc caacttcttc acgaataact gtatgtttgc
    25621 ttacatctac ttgctcttct ttgattggga tagtaatcgt gtcatcttcc aatttttcgt
    25681 ctgttccctg tgaagattga tctgttactg gtttgcgttc gatgacgatt tcttcacgtt
    25741 tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg tcgacttctc
    25801 cagttgtcac atcatgtgta tttacatcta atttttcttc ttttagttga attgtatcat
    25861 catctagatt tggtttgcga tcgatgtctt cagtcttgct tgtacccatt gtatcagtct
    25921 cgccaggtac gtccatcgga tcagttcctg gtgtcatatt caatggatcc gttgtatttt
    25981 gaaagcctgt cgtgccacct acgtccattg gatctgttcc agtgccagtt tctaatccag
    26041 tccccattgt gcttgctggg ctaacagtat cttgtgtatc cattgtatct tctgttgttt
    26101 ctggctgacg atagcctttt actgtgatga caaattttcc atcagaaata tctcctctat
    26161 attgatataa aggatcattt tctggtgtag ccgtctcaga atcataggta tcaaaagaaa
    26221 aagtatcttt tattttttcc cataaagaac ggtcttcttc accatccatc cgattttcat
    26281 ctgtttcaac atctatacca gacaatccgt caaatcctag tctagatgct gcttcatcag
    26341 tagtataaat aacgatatca tctcgttcat acccttcgtt acgcaaccgt tcaattactg
    26401 ataatgcttc ttccctcgta ctatagcttc cgatgaccgt taaatttttc tcttccatgt
    26461 aaattcctcc tagcaaatag atttatgatt ggtatattgt tactta
//
LOCUS       DACDHJ010000018        26268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDHJ010000018 DACDHJ010000000
VERSION     DACDHJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.018"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..623)
                     /locus_tag="IV223_000618"
     CDS             complement(18..623)
                     /locus_tag="IV223_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8710925.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(1073..1693)
                     /locus_tag="IV223_000619"
     CDS             complement(1073..1693)
                     /locus_tag="IV223_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8710926.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(1695..2564)
                     /locus_tag="IV223_000620"
     CDS             complement(1695..2564)
                     /locus_tag="IV223_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8710927.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(2587..2994)
                     /locus_tag="IV223_000621"
     CDS             complement(2587..2994)
                     /locus_tag="IV223_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8710928.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(3013..4302)
                     /locus_tag="IV223_000622"
     CDS             complement(3013..4302)
                     /locus_tag="IV223_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8710929.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(4329..4631)
                     /locus_tag="IV223_000623"
     CDS             complement(4329..4631)
                     /locus_tag="IV223_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8710930.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(4656..6749)
                     /locus_tag="IV223_000624"
     CDS             complement(4656..6749)
                     /locus_tag="IV223_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8710931.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(7238..8002)
                     /locus_tag="IV223_000625"
     CDS             complement(7238..8002)
                     /locus_tag="IV223_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8710932.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(8113..8982)
                     /gene="mdcB"
                     /locus_tag="IV223_000626"
     CDS             complement(8113..8982)
                     /gene="mdcB"
                     /locus_tag="IV223_000626"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8710933.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(9007..10083)
                     /locus_tag="IV223_000627"
     CDS             complement(9007..10083)
                     /locus_tag="IV223_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8710934.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(10107..10700)
                     /locus_tag="IV223_000628"
     CDS             complement(10107..10700)
                     /locus_tag="IV223_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8710935.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(10704..12380)
                     /gene="mdcD"
                     /locus_tag="IV223_000629"
     CDS             complement(10704..12380)
                     /gene="mdcD"
                     /locus_tag="IV223_000629"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8710936.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(12370..12669)
                     /locus_tag="IV223_000630"
     CDS             complement(12370..12669)
                     /locus_tag="IV223_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8710937.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(12771..14417)
                     /gene="mdcA"
                     /locus_tag="IV223_000631"
     CDS             complement(12771..14417)
                     /gene="mdcA"
                     /locus_tag="IV223_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8710938.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(14424..15335)
                     /locus_tag="IV223_000632"
     CDS             complement(14424..15335)
                     /locus_tag="IV223_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8710939.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            15455..16312
                     /locus_tag="IV223_000633"
     CDS             15455..16312
                     /locus_tag="IV223_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8710940.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(16499..16861)
                     /locus_tag="IV223_000634"
     CDS             complement(16499..16861)
                     /locus_tag="IV223_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710941.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            17207..18646
                     /locus_tag="IV223_000635"
     CDS             17207..18646
                     /locus_tag="IV223_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8710942.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(18653..21205)
                     /locus_tag="IV223_000636"
     CDS             complement(18653..21205)
                     /locus_tag="IV223_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8710943.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(21319..22650)
                     /locus_tag="IV223_000637"
     CDS             complement(21319..22650)
                     /locus_tag="IV223_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8710944.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(22616..24094)
                     /locus_tag="IV223_000638"
     CDS             complement(22616..24094)
                     /locus_tag="IV223_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8710945.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(24399..26234)
                     /gene="tdc"
                     /locus_tag="IV223_000639"
     CDS             complement(24399..26234)
                     /gene="tdc"
                     /locus_tag="IV223_000639"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8710946.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         7603 a         5621 c         4504 g         8540 t
ORIGIN      
        1 ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa aataagtaac
       61 tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact aaaaaatccc
      121 atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat cgcacaagca
      181 aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt cgtatcaaaa
      241 actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac ttctttacgc
      301 acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga ccattctttc
      361 gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc acgaacatct
      421 acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc ttcgaaagtt
      481 tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc aaaacgctct
      541 ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag tagtcttttg
      601 tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt atgactatgt
      661 aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat ctgcaataaa
      721 taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg gctgtgaata
      781 aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa aatatgacga
      841 gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca caacctcccc
      901 actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca tcaaacatga
      961 gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg ctcacgacct
     1021 ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt tgttacttat
     1081 tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact tgagtatagt
     1141 ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt cggtcggcga
     1201 tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga tcttccaata
     1261 cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat tcacgatcac
     1321 gtttgtaccg acgttgacat aacacttcaa agtctgctgt taggcgaatc gtgatcacct
     1381 gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca ctaaaaggat
     1441 actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct ttgtaataga
     1501 aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta aacccgatgg
     1561 attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct ggaaatcgtt
     1621 cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc agaagaatga
     1681 aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt cataaacagt
     1741 ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac attatggcga
     1801 agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt ccagttgact
     1861 aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc aggataatct
     1921 tcttctttca cacctgttcc tccatggata actaggggga catcaactac tgcatcgatt
     1981 tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg attcgtaccg
     2041 atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc gtcttccggt
     2101 cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc gccgattgtt
     2161 ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac tgcttttgtc
     2221 ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga agtatatccg
     2281 ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg caagactgca
     2341 tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg catgccgata
     2401 tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc ttttgctgcg
     2461 cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac tgtaaaattc
     2521 aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta ttgccacctt
     2581 atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca tctcatccat
     2641 tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat ctgcacgctt
     2701 ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt tattttccaa
     2761 actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa atttaagtcc
     2821 aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa tctcttgttt
     2881 caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg ctttttcttc
     2941 ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa acatcatttt
     3001 ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat ataagagtgc
     3061 tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg aattaaagat
     3121 cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac cgccaatgat
     3181 cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc cggcaaagaa
     3241 attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag taaacatggc
     3301 aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg ggctataagg
     3361 aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca tagctatccc
     3421 tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca atacatacac
     3481 acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt gatctcctga
     3541 taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata gcgggaccat
     3601 cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg gcaacttgat
     3661 atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa caccggcttc
     3721 aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc cagtgatttt
     3781 acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc cgataaaacc
     3841 accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga ctgtcgtcat
     3901 cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt ttgtgaaacg
     3961 agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat ctctacctaa
     4021 acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga tcccttgcat
     4081 atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga tgatgctcga
     4141 accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga tgacttgtcc
     4201 tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg tgatcgatgc
     4261 ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtttttac cctcttttct
     4321 ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc ttcaatttca
     4381 ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac tgcgaacttt
     4441 tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat ggaaactgcg
     4501 gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa aacatcctca
     4561 gcagacatct gtgctgcaaa actacttcct aaacctgcac cgcatacgaa taaaatgttc
     4621 aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac tgaaaaaagc
     4681 agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt ctcttcgttg
     4741 ttgatcaatt caattagttc tttcatgatg ttcagatgcg agtaggagtc tactgctgcc
     4801 aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat tggttgctca
     4861 atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc atgggctagt
     4921 gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg aatcatcgct
     4981 tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc tagttcaatc
     5041 gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt caaaatatct
     5101 tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc gaataatttt
     5161 tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat gacatctatt
     5221 atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg tagttcttca
     5281 tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat cggatcgaca
     5341 actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag attgacatcc
     5401 aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc gatctgagga
     5461 aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg gttacagaca
     5521 acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat cgtcgaaaag
     5581 tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac ttcttcgatc
     5641 gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg gatgttttct
     5701 ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc agccatgtgc
     5761 ttatagagat tttcgaataa agcttcttcc tttagatgga aaggtacgtg tgtctcgttc
     5821 tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc gattgaattg
     5881 gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct ttctacttgc
     5941 gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga aaagctactg
     6001 tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt cctgattcgc
     6061 tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc ttcttgtttc
     6121 aacaacttat tggaatagat ctcctgtatt tttttgattt ccaccaaagg aaaataatgg
     6181 agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa gtcggtcatt
     6241 cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc tgaaccttta
     6301 taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac cagtgttgct
     6361 cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc ttcttctttt
     6421 ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc aaaaatacgt
     6481 tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa tcgagcttgc
     6541 ttttcctttt ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat ctccggaagc
     6601 tttttactgc tcaaccagat attcgcttca agaacttcct gacggatcgt ccgctgactg
     6661 acattgaact cacttgctag atgttttgct tttactggat attgggctgt catgattctg
     6721 tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt tcaagtatat
     6781 ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa agaaagtaag
     6841 caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa agacatacgc
     6901 ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa agctgtcctg
     6961 catcaagtaa taagaacaac taaactaaaa tagtgcttcc tatttttcgt ttggttgttc
     7021 ttattatcgc agatgcaagc ttttaaacac catttagtgg agaggttgtg acagagatgt
     7081 tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt acaaaatcgt
     7141 gccttattgc cgataggcta tctctggcac actgtttatt cagaaaaagg agctgcgaca
     7201 aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata atcggctgaa
     7261 taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat atctgagaaa
     7321 aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga tcgactgaat
     7381 aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc tgtcgataat
     7441 ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct gctttgtttt
     7501 tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt ctggcttttt
     7561 caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg atttccagaa
     7621 actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc gctttcagcc
     7681 ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct actgacaaaa
     7741 cagctaatgc aagaaatccc gtttgctcat aactagaagg acctgtacct ggaccataat
     7801 acccataaga atctctgccg taagaacgac tgccaaaata actgccccca tatgaacgac
     7861 caccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca tgaccacccg
     7921 cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac aaaatgattg
     7981 ctaaataatt gatttttttc attgtcttcc tcgttatctt ttattacaaa aaactatttt
     8041 tatgatagcc ttgctaaaag gaagagtcaa taaccagatg cataaactgt ttttttagca
     8101 attttgtctt atttagtcat tgagtaacat cggacaaatg tatgtgtcgg cagtccataa
     8161 ttcctctatc ttcagcatga acaagcttgc agacaagaga tctgcgcttc ctccaggaga
     8221 gatttggttt tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg gtaaagcact
     8281 ttgactagca gcatgagcaa gtttttgcat ttccactaac gttttctgat cactgcgatg
     8341 caatacgcat gtgtcatcta gcgaactcat caaaactaac aacatatgca tttgtttctg
     8401 tacatatgaa tcattatcta gctccttagc tgcttggatt gcaagcctga tatgcggata
     8461 ccctaatatc gcttcttcat atgcaccatt tacgccatat ttttgcttca ccctatgacc
     8521 atgagtggtt tttttatgac gatattttgt atcaggaaaa gcagcaagct tgctgacatc
     8581 atcaagtatt ttcgataaag aaaaaggctg ttccatactg attttttgtg caatgacact
     8641 gatcaatagc cccattgccc aaatcgctcc tttatgggta ttgatgccat ttgtagcaat
     8701 cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt ctttctctta actcctgatc
     8761 aatagcatga tgccatgcta tttgagccat ttcttcaaaa aaaggagtta gcgtcttagc
     8821 agacttaaga aataaagcat agtccatatc ggaatgtgca cccgcatcca gtgcatccac
     8881 taatccaggt tttggactta atgttacttc tgcgatcaat gcttcctcta ccaacgcagc
     8941 gagttgctga gtcactgctt tttttcgttc gtcatttatc atgacactca ctccttcttc
     9001 ctactattac ttgctgaaac cttttttgat agctacccaa atgatatgaa gtaaccagct
     9061 taaagcaaat gcacaacttg ccgtcattag ataagctaca agaatcatga ctcctgaagg
     9121 taactgcagc caactatctt taaacaataa taacagtact tggaaaacaa atacattggc
     9181 taaatacgta cgataagcat aaacagacaa ccatttgaac aatggcaatt ttggcgaatc
     9241 gtttacaatc attttggaag cgcctatgaa gacagcaaag atgatgacta aactgtataa
     9301 actttggatc gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg gatgtgcgag
     9361 caatgaacgg acagaaaaga acgcgataac taaggcaaca ggtaagaaaa gataacgtac
     9421 tctgtccaaa tattgataga ctttttcgtg ataaacaaat gcggctaccc cataaatcat
     9481 gaaactagca acaaaccgat ctaataagta ccagctgtca tgataaggcc cagtcaatac
     9541 ttgtgtttga tagaacagat accatgcgac atataaaatc gttgaaccaa tcaatactgg
     9601 ccatacatat tttttattag caaatacttt gtaaccgagc caaacaaaaa acggcatgaa
     9661 caattggatc tgcagcatca taacggtata ccataaatga ggcgctccat cacctgtgac
     9721 atatttcagt aaaaagcttg cagtatccgt atacggagtt tgttgttgga ctgctggaaa
     9781 tagccaaaga taagccgtcg tccatagaag atacggaacg actagttcaa aaaacttttc
     9841 tttcaaaaat tcccaataag acaaatgctg acttgttttt accatattat aaacaattgc
     9901 aaaaataaac attggtgctg tgtatttaac gatgacgtaa aaggtaccaa gaaaagctgt
     9961 atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca tcataacagc
    10021 cgttacagct gatactttct ggaagtcact tacgttgatt ttgatttttt tgttttcaaa
    10081 catctctttc tctctctttt tctttattac caaagttctt tggttaattt aggtccttcg
    10141 actgttttca atagaatttt tttctctggc tctctcaaat actcttttag tgaaaaaccg
    10201 ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg cgattgaatt
    10261 acctgaattg gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa atcactggtt
    10321 tcttttactg ccggaatccc agtcgataat tcaaatccta agctgccgcc gattccccat
    10381 ttacatcctg ataatatgtt tctagcagca tgaaattgct gataaacagg ataatccatg
    10441 attttctctt gtctatacga ttcttgttcg atgatgtccc acggatgaat gacttgtttg
    10501 acggcatgtt ttgagatttc tgttgaccat ctctgattgc gggcaggtcc acgtaaacca
    10561 atcggtagta tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa catcttttca
    10621 gttagaacga cccattcagg caaggtgaac ccttctattt catgtttatc aaaaaggaca
    10681 atatcatgtg cttgaatcat tttttatgca tcccattctt cgtccatttt ggcacggact
    10741 ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg atttgtcagc
    10801 atcgtgtcat ttgtattgcg gacatcttga atggcttcat tgattgcttc gatgactttg
    10861 ctgacatttt gttctgaagc agcttggtcg gtgatatctt ctaggaaatg atagatcgct
    10921 cctagttttg tataattatt gatatcgtaa gcgatagctg gaactttttc agcagctgct
    10981 tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac tttctttaga catcgcttgg
    11041 acggtaattt ctttattgtt taaagcaatc aagcggcttg attgtaatcc atgtgctaaa
    11101 aatgcacctg aaatagcatt tcctgcaatc aatccaataa ctggatgtcc agcttgacgg
    11161 agttttgcat aagcagcagc actattagcc aaagccacat ggataccgat caattcttcg
    11221 ttatagccgt atgcttggct tggtacatcg atcacaacga tgattggccg tttcactttt
    11281 ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa accttcaact
    11341 aaaccaactt caccgtttcg tacacggtac atcgggtttt taggatcagg tacgattgat
    11401 gtcacgatgg cttcttctat gaatttactt gatttaccat aatacacagt actaatagaa
    11461 gaagttggat ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac acttgctgca
    11521 gctggttcat ttccttctgc tacttctggt aagtcaattg attttgcttg atggttagca
    11581 attgcttcat tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga atcaagtaat
    11641 gataaataaa atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc cattgctgcg
    11701 atgacagcag ctttgattgc ttctacacta tcagtcacta attcatcaat gatttttgat
    11761 tctacccgtt gacgagatcc taatgtattc caaatcaagt ctttgtcact agaatcaaat
    11821 tctcgaacgc ctgcttcttg ttcaataact tctggtccat tcaaaccgac tctcgctttt
    11881 tttgttgcga ttaaataact cattaatgct gaagtaatcg acataccgcc aaatgatcct
    11941 acacgtcctg gaatcaaacc gatgactggt acatattgtt ttagagcaat gattacattt
    12001 ccgatttctg aaatagataa cagtccgtag tttgcttctt gcaaacgaac accacctgta
    12061 tctagtacaa tgactggaaa gatctcattt ccttttttat tttcttctaa cacatgttca
    12121 agtgcagcaa cgatttttgc accggaaact tcaccgatac cgccaccttg gaattttcct
    12181 tccatagcga ttactactgc ttgtttgcca ttgatcgtgc cacggctgac aacgatcccg
    12241 tcatcacttt cagggacgat tccttgtgct ggtaaacttg gcgcgatgat ttgatcgaat
    12301 ggatcaatca attcacgtcc attgtcaagt agagcgttca ctcgttctct ggcgtttaat
    12361 tctacaaaac tatttttcat ctgaaagttc ctccattgct tgcgtcaatc gtaggtacac
    12421 aactcctggt gtagcaccga agtcattgat cacgatatct gctgaaattg gataacgatc
    12481 aaaaaatctc gttaatacat tgttccatac ttcgccgaag ccatcgcttc ctgtacaaat
    12541 ttttacagta gattctgttt tttctgttgg aaacatcaag atttccaaat ctcctgaacc
    12601 gaccactcct acatggattt tcttattgat cggctgtttc gttggatatt tataagctaa
    12661 ttgttccatg gatctctccc ctttttgtaa aacagagcat acacgttctg cttattcgtc
    12721 tttcttcttc catctgggta aatggcgaca aaaggttttt ctcgccaatt ttatgaccaa
    12781 gttctgaatt tcattggtgg ttcatacagg ccatctgacc attcgatcaa atcatcgatg
    12841 gtttgtgcag ctaataatga acgtttcgct tcactacgtt tgatgcctag atcttctggt
    12901 aatttcacga tgccggctgc acgcaagcta tctagtgatt ctttcgtgct agtcattcca
    12961 agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc gttcttcttt tgtttttgct
    13021 ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg tagtatcttc gctataaatc
    13081 atcactggtg cattggctaa gcctgcttgt ttttgaacag ctagtgcatc taattcttcg
    13141 acaaagactg gttttttatt tgaaccatat gtttcaacca tttgaacaac taatttttgg
    13201 cctttcccta atggatcatc attgtcgcga agtgattgcc atgctggtgt agaatgacga
    13261 cgaccgccag gattatgtcc catgttaggc gcaccgccaa aaccagataa acgtcctttt
    13321 gttacggtag aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat aaacatatcg
    13381 atggcatatt gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt gccgtcttta
    13441 ccgacaaaaa agacatctgg gcgagcagca atatattttt ccataccaac ttcgccacca
    13501 aaactatgga tgctttcaac ccaacctgat tcgatggctg gaatcatcgt tggatgtggg
    13561 ttcaataccc agttcttcgc gattttccct tttaacccta gtgattcacc ataagttggc
    13621 agtaacaatt cgatagctgc tgtattgaat ccaataccgt gattcaaaga ttgtacgccg
    13681 tgttctgcgt agattccttt gattgccatc attgccatta gaatttgcaa ttctgtgatg
    13741 ttttgaggat ctcgtgtgaa taaagcttct aattgataag gtttatctgc tttcacgaca
    13801 acgtctaccc agtcaccagg gatatctaca cgtggtaaag aatcgacaac ttcgttgact
    13861 tgaacgatca cgataccatc tttgaaagct gcagcttcga tgattgttgg tgtttcttcc
    13921 gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc tactaaaaca
    13981 acatttggaa tcaaatcgat gaataagcgt ccataaagtt ccaaataagt atggatatca
    14041 cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg tgcaccagca
    14101 taagagaaat cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg ttctggtcgt
    14161 gagatactag acatgatcaa atgcagatca ttcacttttt ctgcatctac tttttcaaga
    14221 ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac tttgtcgccg
    14281 ggagcaatca gtttttccat tacttctacg attttttcag tttctacata cttgccattg
    14341 agcaagttgc tgattttttc caatcgctgc gctttttttt cgcgtttaga agcccagttg
    14401 cggtttggtt tttccataat tagtcatctc catttttgat atagaaacgt ggcgtcatca
    14461 ataccatgtt ggtctagatt gatcgtacgg atccctttat ctacaccaaa ttttgtatga
    14521 atcagctttg tcaaagtatt gccgggggga aattcaatcg tcagatccat tcctaattct
    14581 tttgccaagt ccatcatttg gttccactgc acagggaaag ctaggttttc caacaaatct
    14641 ttacgaatct cttctgcgtc ctgagtggct cttccatccg tattttttat atacaaacat
    14701 ctagcatcag atagatggac agactgcata tacggcttga atttcttgac cgtatcattc
    14761 atcaagagac agtgagatgg cacagggact ttcagcagct tcgctgtttg cgcttggtgc
    14821 cgtttggctt ctgccaacgt aacggataaa ccttcgatac tcccagataa cgtatgttgc
    14881 atcggacaat tttcatttga cagataaact ggatggtcag gatcaaatgt ttttgctaca
    14941 atttcctcag cttcgcttct cgtcacacct actactacgc ccattgcata accgcttgga
    15001 taagcttctt tcattaagac tgcgcggtga tagactagtt ccgctgcatt ctcaaatctc
    15061 aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc tgcaatatca
    15121 ggctgaatac cgtttttttc catttcatct aaataaaata ctgcttttgt cagtaatgct
    15181 agctggataa aaaccgtatc ttgatagttt tcttctttat caactaaccg ataccccgtc
    15241 acattttcta aacgttccat gtacatttca gggatatccg ctaacatccc taacttctga
    15301 ctgccttgtc caggaaataa gaaacctgta ctcatgagcc tcgcctcctc agcacttact
    15361 ttttccactg ctatcttagt caatttctgc tataataaaa aatgccaaaa aaagaaaaaa
    15421 ccataacaaa aaggtatgga tgggatggat aattatgaaa ttgcttcatc tgcagtattt
    15481 taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat ttatttctca
    15541 acctgccttg agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc ctttgtttta
    15601 ccatagcggc agaaacattg agacgaccgt ttatgcagaa gaattttatc catatgcagt
    15661 caagacatta gacacattag aagaaggaat caaattattg tcttcggtca atgagaagat
    15721 cgttacttcc gtcatattgt atttagaggt agcctctgtt tctattccga acttggtccg
    15781 aatttttatg gaaaagcatc ctgatatcca attaacgatc atgcaacatc atttaccaga
    15841 tgatattacc gatcctgtac tgtacatcac ttcggagatg aagcctggca tgaccaatat
    15901 tcctatcatg aaagaacctg tttatgtcgc cattcctaag aaacatcctc ttgctaaaaa
    15961 agaacatctg caattagaag atatccaacg cacccccatc ttgatgctgt caaagagcaa
    16021 cgccttccgt catacactgg atgcagcact tgaagcgaaa agtatcgaat tgacgatcag
    16081 ttcgacgaca gatgatcctg cgacattacg ttctattgtc aagcaaggat tagctgttag
    16141 ctttttccca aaagtttctt ggtcttacga taaaaatgat cctttcgtct tgcgggaaat
    16201 caaagatttg cccttgacac gaacaattta tttatcttct agcttcacag aagatcatcc
    16261 tttgatcaaa atgattgcaa aaacgatgcg ggaattttat ttaggaaaat aaaaaaaaaa
    16321 gagctgggac aaaattcttg tcgcagctct tttttcgaac aaacggtgtt ccagaagtaa
    16381 tttcttagaa aataagccga aatttcccaa aaatttgaga agcaattttc gtaaattcct
    16441 tcttatttct tgaagctaaa catttctgtc acaacctctt ctctattgtc ctagacattc
    16501 attgcgttga tttaaaactt ccatcaatca aatctgctag ttcatctagc ttttctattt
    16561 tacgtagatc aatagagatc caatgttctt tattcatgtg ataggctgga aaatacccct
    16621 cttttcctcg caaaattccg ccaagttcag gatcgacctt caaatcaaga atattgatga
    16681 tcctatggtc acttgcatca tcatagattt tttcttctgg aacagacatc aggaccccaa
    16741 accatttttt gcttttttga tgtctgaata cgcaataatc aggaaatttt ttaaaaatat
    16801 attctggaga caaattgtat gtttcattga tgtgatcgat tagttctttt ttcgttagca
    16861 tctgcttcca tctctttctt attgattcgc tttattcaaa gataccacgt aagaatagaa
    16921 aaatgaatac aagtagttgc taatcaaata cttcgatatt tttctttttt atcaactctt
    16981 aaatgttcaa tccgcctgtg ttggtcttgc taccatctac tattaaaatg tttattattg
    17041 tttgcccacc tattttttga ttttagtggt atcatttttc aagttcatga tgaaattctt
    17101 atcatcagta aatgctccag aaaaataaac aatgattgaa aaatcagttg aaacttgacc
    17161 accgtttatt ttctccgtat tcttagaata gaagggagtc tgattgatgt attcactaat
    17221 gaaaaaaatc attaccgaaa aagatattcg acgtcatgtt tctttagtag aacaattgct
    17281 gaatcataca aagataaccg taaatgaatt agctgaaatc atagggacaa ccgaacgaac
    17341 gattttttct gaccttcaat ctattcgctc tcagctgccc gaaggctggg acatcatcag
    17401 cgatcaagca ggaattagtt tgcaaaacca gcaaaattta ttaacaaatg acctttggga
    17461 gatttttttc aaacaatcag tcagtgtcga actgctgaaa aatctgcttt ttacaaaaaa
    17521 agttgctgtc cctgattttc tagccgacca tgggctttct tatgggacat tgaaaagaca
    17581 tgtgactaaa atcaatcagc gtttagcaag ttacgacttg cagatcgatt tgacgaaata
    17641 tacagcttgt attcttggaa aagagcgtgc gattcgtact ttttatcatc gattgctgat
    17701 tcctttcact cataacaact attttttcga ggattattcg atccatgaat cccattattt
    17761 tcaattttta agaaacctca gtcaaactga actagcggtt gaaacagaag agattttcgg
    17821 aacatgttgg ttctttatca atacgatccg tataaaagcg aattgccgat tagattcttc
    17881 tatccatacc aacagcacac tctcctccct gtatgactct gcattgaaaa aattgtattt
    17941 aacggaaggt atttatctta aagacactga gctttctttt gcttctttct gctttctgga
    18001 aagttggaat tacaacaata actatggaca agaaaccgct cgctgcctgc atcattctcc
    18061 atttttagag gtcctcgaaa cgtttgtaga ggaactagct tcagagctgt ctcttgatca
    18121 actaaaaaaa acatctctga ctgataactt ggctctcctc atattaaaat atcatgaatc
    18181 gccgatattg attgaagcat tggaccgtca ataccatcgt tttttagaaa catatgatca
    18241 gcaatatgca cacttgtatc aacagaaaga gaatttactg aacgatctaa aaaaagaaat
    18301 agaaatcaat gaaccaaatt actttctcca actcttgtct ctactgatcc aacaaacgat
    18361 tttttctatc aaaccgaatt tgttcaatgt ctattttatt ttccaaggag aaccttcatg
    18421 gaaggcgttc ttgcagcagg aattaaaaga ttacttagga aaacgtgtct gcttcttacc
    18481 tatcgaacta accgatcttt ccactatttc ttttgaaaaa acagatatct tgatttccaa
    18541 tttccctcta gattctctag atatccctat tgtatacatt tcttctattc caacgaaaaa
    18601 tgagctcaat cgtttgactg aactcacttt caaaactttt ttatagtaac ggctagcgac
    18661 tgtattctct agctgttttt tcttttttgc gaaacagttt gttcatgatc cctttttcta
    18721 tttctacaac taagaataga agaatcacat ttgctccaat caataactgt tgtgtcatcg
    18781 ttaaacttac tgtaccgatc agctgctggc cgactggtag aaaaataacc atcccttgca
    18841 ataaagcaag gatacctaat gaaagaaaaa gcgctttgtt ttgcaaaaga ccttggttca
    18901 atgacggctt ggacaactct ctgcaattga tcatgtaaac agcttgagct aagacgatat
    18961 tttgcagaag aatggtttgc tgtaagcttt gcccttcaaa ctgcatagcc aataaatagg
    19021 caggaaccat gatcagtagg gagacataga tgatccgaaa caaatgataa ccagatagga
    19081 ttccttgctt tggatctctt ggtgggcgct tcatgatttc tggatctggc gactcgaacc
    19141 ctagttcata agataaagtg attgtcgtta ccatattgac ccataaaatc tgtactgggg
    19201 acaaaggtag cgggcgattc agcataagtg cccaaatcaa gatcaatcct tgagctaatg
    19261 cagttggcaa gaagaaattg atggtttttt gcaaattatc caaaatccgc cggccctctt
    19321 ttacggcttt tgcaatcgta tggaaattat catctcctaa aaccatatca gctgcctgtt
    19381 tgcttacttc actacctttg attcccatag caattcctac atcggatctt ttcaaagcag
    19441 gtgcatcatt tacgccgtct ccggtcattc cgacgatttc tccatttttc tgcaaagctt
    19501 taacaattcg cagtttatgt tctggtgtcg ttcgagcaaa gacatcgact attggcactt
    19561 gctgaatcaa ctcttcatca gacataagat ccaaatcgat cccttctaaa actttggcag
    19621 tatgttttaa gccaatttgt tccgcaatcg cttttgctgt gtctttgtga tcccctgtaa
    19681 tcattttcac tgagatccct gcttgttggc attcctttac agctttgact gcactttctt
    19741 ttggcggatc gatcaatccg gcgatccctg caaaagttaa ttgggttaaa gtctcatgcg
    19801 tgagttcctg tttcgaatct acttttttat aagcaaatcc taaaacacgc tgaccttttc
    19861 tcgcaaattc tgcagctgtc tgactccatg cttcttgttc tgcagaagaa agagttgatt
    19921 tagcaaataa tacttctggt gcacctttga cgtaaatcac cgctcctttt tcctccgcat
    19981 gcatcgttgc catatatttg tattcagaac tgaacggaat ctttgcttct atttttttca
    20041 aaggtagatt ggctgtttct gtgtgtcgaa tcagcgcaac ttctgtcgga ttgccactta
    20101 aattttctgt tttctgttct tcttttgttt ttaattcctg acagtttttc ataatggaca
    20161 acacgcaggc ttcttctttt gcagctacat ccacaactgt catttcattt tttgtcaatg
    20221 ttcctgtctt atctgaacag atcactgtca tagaacctaa tgtttctacc gaaggaagtc
    20281 ctttgatgat cgccttttct tttgccattt catgtactcc aacggataga atcatggtca
    20341 aaacagctgg caatcctttg ggtaccattg acaccaccaa agcaatcgta gccgaaagaa
    20401 ggatatgcca atccatacca tatcgaaatg tcgtaaaaat aactaagaat aggctcaaac
    20461 aaagaatgcc ttgaaaaatt tgtttgttta atcggttgat ttttttaatc agaggcgtcg
    20521 tttgttgttc gaccgattgc aatgcttgat tgattttacc gatttctgtc gtgtttcctg
    20581 tttctacaac gacaccgact gcagaaccag attgtacaag cgttcctgaa aaagccatat
    20641 ttttctggtc gccgatcgat tcttcttctg tcaaaacggc atgacttttt tcagatgctt
    20701 ctgattctcc cgtcaaaatc gattcttcca ctaataaatc atgtgcttcg attaatcgga
    20761 tatctgctgg aacgacatct ccggcacgta agacaatgac atctcctttt accagttctt
    20821 tcgcagggat tttttcttgc atcccgtttg atagtactat cgcttcttgt cccatcagct
    20881 gcttcaaacc attcaatgat tcttccgctt ttctttcttg ccagtaacta acgagtccat
    20941 tgacgatcac tacgaataaa ataatacttc cttcaatata ttcactagaa gcaaatttca
    21001 aaaaagaggc aatgatcaac acgatcatca gcagatcagt aaaatgcttt gctatttttt
    21061 tccatcgttt tgtctgctgc cttgctttta tttcattata tccttgatca gtcaggcgtt
    21121 tctgtcgttc ttcttgcgac aagccttctg ttgatgtttg atagtttttc aatacatctt
    21181 ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt ttaattgata ctgttattat
    21241 acgcagggaa gttttttcag gcgtatcgat tatttcaggg gggtctgaaa taattcgttt
    21301 tgcaaaatgt atctgggcct aactgatatt tctattgctt attcttttat atacaaaatt
    21361 taagaataca aaccagataa ccaataaaaa aggaataaag cgtgtcgtgc tttcgaataa
    21421 caagctgaaa gcaattgaag taaaaaacac caacaaagca agatctttat atctttgtgt
    21481 agtgttccgt ttcttttctt taataaattg aacatgtgca cacaagatcg aagaccacac
    21541 aaatagaaaa caactcgttg tcatgcctgc caaaagctga aaaaacaatc gactatcagc
    21601 tacaaaaaaa tgtagacaaa gaccggcaaa aaataacatt cctgtaatta aaataccgtg
    21661 taaaggtacg cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt tttcttttgc
    21721 tgtttgatat aaaatcctgc tagtactgta gactgcacta tttgctgagg acaaagaagc
    21781 aaccaaaatc attagattga caattgctgc tccgccttgg caatggatca cttgcagcat
    21841 agtcactaaa ggactttctt ttaaatctaa acaatgccat ggaacaacaa ataaaatcga
    21901 caaaatcgtt cctatataga aaaagagaat acgccaaggc agacagttga ttgctttttt
    21961 caaattcttt tgcggatcac ttgtctcgcc aacggttaag ccgatcattt caattccagc
    22021 aaaactgaat aaaaccatag gaaatgagac taccattcca gcaaagccaa acggtagtaa
    22081 ttcatcgatc tgtccaaaat tttctttgat tccgatagac tgggtatgaa aatctgagac
    22141 actcaatcct atacccaata agataaaaag gaaaatcccc aagatcttga tgattgagaa
    22201 aaaagattcc aattcaccaa accacttgac tgcaacaagg ttgactgcca ttaagatcat
    22261 tagtgcgcta cctccgctta tcaaagctgg tacatgtaca aaccagaaat ggatatacaa
    22321 tcctattgct gttaattccg caagtccagc tccaagccag caaagccaat aagtccaatt
    22381 gacaaaaaat gcccactttt tgcctaaata atagtcaacg aaatcactga tagagttaaa
    22441 tgtactattt gctaaaaata aatcacccat ggcccgcatc aaaccataaa tagccaaacc
    22501 gcatatcaaa taaacgagaa taacagctgg ccctgctgat gcaattgcct ttgctgaacc
    22561 taaaaataat cctgttccaa ttgcgccacc taatgcgatt aactggatat ttcgattagt
    22621 tagagtccgt ttcattgccg gtttttccat caagttcacc tttttctttt tcaagcagca
    22681 tggcgacttc ctgttcccaa gaaggtttct ttattttttc aatacagagt ggtactacaa
    22741 acaaaatgac actgatggct gtgactgcaa ttagattgat ccatgttcca gctttcatcg
    22801 tcagtccgac agaaaatccg attgccgcaa ataaaacaat aacgacagtc caaactccaa
    22861 gattgctctt tttctctttt tttccaattc taaatggtcg attcaacttt gcatgtgtat
    22921 aacgcaatct caaaactgcg atccccatga tgacatagac gatacagtag ataacagtag
    22981 ttgaatttac gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa taaaaaagtg
    23041 caaagattga gataatgaca gactgagtca aaataacgga tttggatacg tcaaattgat
    23101 tcgtcttcca aaaattgaat tttggtggat acgcaccttt tctagcactt tctgtcactg
    23161 tctttgctgg tcctgaggcc caagcagata attggactgc tacaccaatg aagacaagta
    23221 aactaaacag attcacaata agatgtggca aaccaagaat ttggcaataa atcgaaatag
    23281 attgcgcgat attattcaat tccatttgtc cttttggtac gacatttgct acaactaaag
    23341 cattcaaaat attgacgaaa aatataaaaa ttaacgcggc aaatactccg cgaatatacg
    23401 ttttaactgg attttccaat cgagtgatat acacagagga catttcgata ccagtaaaaa
    23461 taaacatgat cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct ggtattaatt
    23521 tgtcccatga aaaactgcct aatgttccgg ttggtacgat cccaactttg atccacgcag
    23581 caaaccctaa taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa acaccgactt
    23641 ttccgccgat ttttgccata tcaaatttca aattcaaaaa agtgatgatc caataaacga
    23701 ctaaaatgac aactaatgta aatttgctgt tcaaaccaag cggcacatta tcgataatat
    23761 taccaaacaa cggagcgagt gctgaagcga ccatcaccat cccaggaaac atttggaccc
    23821 ataacagcca tgagacaacg aacccccatt tttttcctag agcattcgat acccaaagtt
    23881 ctggaccacc tttttgggga aacattccag caaattcacc agagatcaat gagattggaa
    23941 aagcgtacaa taatactgca accagcatgt aaaaaatcat cgtccagctc gttgctgcta
    24001 catctggaat atttcgtaca cttgctacaa gggcgcaggt cattccgata aaggtcatgg
    24061 tcgtgatttg ctttgatgaa ccagtatctt tcatctttat tccttctttc aatagcgaca
    24121 tatgtttttg ttttctttta tttttgttca agaacagaaa tgacaaggtt tcatcgattc
    24181 agtacaaaac ccagtatcac cgaaacatcc attgaacgat cttagtgaac tgggtttgat
    24241 acatgaaacg aacgatccaa cttatagaac gttttataaa aaagaatatc ttggcgagga
    24301 atatatgaaa aacaggggga aatacaattg ttttgatgaa gcaaataatt gtctgttctc
    24361 catatgtctc ttgttattct ttcacacaaa aatcagttct attggatgat ttcgtttagt
    24421 ttcttttcca ttgctttggc gatttttttt gtataaaaat tgaaaattcg atcatcattc
    24481 aaataaggag tcatgatagc cgcacgtagc agagtgattt tctgttcttt tttccattct
    24541 tctttcgtta gacccattct ttcaacaaaa cgaatcggag aatcaccgta atcttcttgt
    24601 gtaaatgtag tatgagaagt aataaaccgt tcaccataaa catcgccatc catataagaa
    24661 gaccgatcga acattttttc atttaattca ttgatagcgt tcaaatctgt acagccttgt
    24721 tctttgaata cccaattgac catattaaaa tcaggatgat tcaatggata gacttcgatt
    24781 gtttttcctt taacagtaaa agtcagatga tcgagaaatt ctctgaatct ttgagctgcc
    24841 tcgattgaag ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa tacacgatgt
    24901 gctgtccaga ctgcggcggc ggttgctcca gcttttgatc cttccaaaat ataagctcct
    24961 aacatatctg gagcctttac tgatttttca aacacatatg gcgcgaaata agaaatgata
    25021 tttctcatat ttttatgttt gattacgatt cctccagcag cataagggac atagcccatc
    25081 ttatgaggat ctatcgtgac agaatccgct tctgaaatag ctctgaaccc ttcatatacc
    25141 tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa ttcagctaag
    25201 gaagcatacg gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc atagccgcca
    25261 tatgcggcat caacatgtaa atagaaatag atcccttcat cttttaacct ttctcttaat
    25321 tggacgattt tatctacact gtctacttga ccttcttctg tcgttcctac gacagcaacc
    25381 acacctagaa ttgggatttt ttgtccagct aattcacgaa ttgttttttc caaagcatta
    25441 atatccattc gataatcctc ttgaactggg atagcaacca tctgatctaa gccgactccg
    25501 caaatatcta acgccttcat ccaagaatag tgttttgttt gagggaccag ccatttccct
    25561 aatctttgga tattttttcc gctacgggaa gaagctgctt tgacctcatc catttcttcg
    25621 tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag ataaattcaa aagttcccac
    25681 tcagacattt ttttcacttt ttcaggcagc acttccttga cagctaaagg aatcgatttg
    25741 atgcaacggg cgtaccataa tccttcaaga ttagcaatag aaccatctgc tgcaatgtgt
    25801 ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc tgcttccatt
    25861 tgcgaagttg ccatagaaga ttccaatgca acattatttg ggttccacag catcgcatag
    25921 ttgtaagcta ataaagcagg catcaatgtt tcagcattca tttgtcccca gtaacggcct
    25981 gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac tgacttcatc caagacctct
    26041 atcatatggt cgcgggtggc tagataatct ggagtgagct tatctccttc gctaattgca
    26101 ggcatatctg aaggaatata attttctctc catccaagat gttcatctac catcttgttc
    26161 aacaacattt tataaactgg tccattttca gctttgtcgc ctataaagac ggccttgata
    26221 tccatatctt tcatttttct tccctccata ttttgttggt caatgtct
//
LOCUS       DACDHJ010000019        25498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDHJ010000019 DACDHJ010000000
VERSION     DACDHJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.019"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..626
                     /gene="aac(6')"
                     /locus_tag="IV223_000640"
     CDS             78..626
                     /gene="aac(6')"
                     /locus_tag="IV223_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8710947.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(1186..3078)
                     /locus_tag="IV223_000641"
     CDS             complement(1186..3078)
                     /locus_tag="IV223_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710948.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(3075..4802)
                     /locus_tag="IV223_000642"
     CDS             complement(3075..4802)
                     /locus_tag="IV223_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8710949.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4818..5468)
                     /locus_tag="IV223_000643"
     CDS             complement(4818..5468)
                     /locus_tag="IV223_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8710950.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            5672..5968
                     /locus_tag="IV223_000644"
     CDS             5672..5968
                     /locus_tag="IV223_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8710951.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            6160..6780
                     /locus_tag="IV223_000645"
     CDS             6160..6780
                     /locus_tag="IV223_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8710952.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6872..8038)
                     /locus_tag="IV223_000646"
     CDS             complement(6872..8038)
                     /locus_tag="IV223_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8710953.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(8050..8403)
                     /locus_tag="IV223_000647"
     CDS             complement(8050..8403)
                     /locus_tag="IV223_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8710954.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            8531..9553
                     /locus_tag="IV223_000648"
     CDS             8531..9553
                     /locus_tag="IV223_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8710955.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9992..10426)
                     /locus_tag="IV223_000649"
     CDS             complement(9992..10426)
                     /locus_tag="IV223_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710956.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(10536..12395)
                     /locus_tag="IV223_000650"
     CDS             complement(10536..12395)
                     /locus_tag="IV223_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8710957.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(12513..13673)
                     /locus_tag="IV223_000651"
     CDS             complement(12513..13673)
                     /locus_tag="IV223_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8710958.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13980..15404)
                     /gene="araA"
                     /locus_tag="IV223_000652"
     CDS             complement(13980..15404)
                     /gene="araA"
                     /locus_tag="IV223_000652"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8710959.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(15420..16118)
                     /locus_tag="IV223_000653"
     CDS             complement(15420..16118)
                     /locus_tag="IV223_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8710960.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(16075..17736)
                     /locus_tag="IV223_000654"
     CDS             complement(16075..17736)
                     /locus_tag="IV223_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8710961.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17954..19354
                     /locus_tag="IV223_000655"
     CDS             17954..19354
                     /locus_tag="IV223_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8710962.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(19606..20685)
                     /locus_tag="IV223_000656"
     CDS             complement(19606..20685)
                     /locus_tag="IV223_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8710963.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20771..21730)
                     /locus_tag="IV223_000657"
     CDS             complement(20771..21730)
                     /locus_tag="IV223_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8710964.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21742..23265)
                     /locus_tag="IV223_000658"
     CDS             complement(21742..23265)
                     /locus_tag="IV223_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8710965.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(23275..23838)
                     /locus_tag="IV223_000659"
     CDS             complement(23275..23838)
                     /locus_tag="IV223_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8710966.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23844..24707)
                     /locus_tag="IV223_000660"
     CDS             complement(23844..24707)
                     /locus_tag="IV223_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8710967.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24721..>25498)
                     /locus_tag="IV223_000661"
     CDS             complement(24721..>25498)
                     /locus_tag="IV223_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8710968.1"
                     /translation="SFTDLNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLET
                     PTQIIVGLLAAVALSKNTRFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLF
                     SQVGLPIPQFFRDPTLAMPTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGAT
                     NRQQFFNITLPQIVPSLGFCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRG
                     FTAPYELGYASALSSVLFIIVATIAISTNVYMSRKEKQMK"
BASE COUNT         7493 a         5302 c         4537 g         8166 t
ORIGIN      
        1 gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag
       61 aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag
      121 atcagctttc tgatttactg agactgactt ggccggaaga atatggagac agctcggcag
      181 aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg
      241 agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc
      301 cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact
      361 tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag
      421 accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt
      481 ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg
      541 taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga
      601 ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta atcagcttca
      661 agaaataaga aggaatttac gaaaattact tcttgaattt ttgtgaaatt ccaacttatt
      721 tccgaagaag ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt
      781 cgagtagtaa gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa atttggctta
      841 ataccgagaa actggctttt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac
      901 tccgagtcac aaggagcaat ttcaaaaaac agcttctcag gtttgttgac tattgtgact
      961 tgtgaccgag gagttggcag gagaacaccg tttaaggaag aggttgtaaa atcagcggtt
     1021 tgacctgagt attagagata caaaacggaa aaatagcttc tcatattttg ccgttttttg
     1081 agctaatacc gaaggacagc tgattgaaca ccgtttattc ggaaaaaggg actgtgacac
     1141 gacttttgtc acagccccta aaaaaaataa taaatttgta ctattctatg aagcttcttc
     1201 ttcagaaaac tgactgttat aaagatctgc atagaatccg tctttttcca tcaatgtatc
     1261 gtggtttcct gtttctacga tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg
     1321 tattgtagac aagcggtgag caacaacgaa acttgtccgg ttttccaata ggcggttcat
     1381 cgctttttga atcagtattt ctgttcgtgt atctacacta gatgttgctt catcaaggat
     1441 caggacatct ggatttgcta agaatgcccg cgcaattgtg attagctgac gctgcccttg
     1501 cgaaatattg cttgcatcct cgtttaatac agtgttatat ccttctggca atttacgaac
     1561 aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc
     1621 gttcccatat ttgatattgt catagatact tcctgtaaac agccaagtat cttgcagtac
     1681 cattgaaaac tgtgcgcgca attcatcacg tgaaagatca cgggtatcta caccatcgta
     1741 acgaatacta cctccagaaa catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt
     1801 ccctgcacca gtcggaccta caatagcgac catctcacca ggtttgacat ccaagttaaa
     1861 atcagtcatt agcaagttat ctggtgtgta gccgaattga acattttcaa aacgaacttt
     1921 gtatggtgta tctttttctt ctggaaggcc agatttttca ttggacatct cttcttcatc
     1981 caacacttca aatacccgtt cggctgaagc aactgttgat tggatagtgt tcatcaaact
     2041 tgcaagttga gtgattggtt gagagaactg tgttgtatat tgcaagaacg cttgaacatc
     2101 ccctaatgtc atgttcccat ttgcaacttt tactccgcct agtacagcga cgaacacata
     2161 gcctagattt ttaacgaaat tcatcaacgg catgatgatt gccgaaataa attgagcttt
     2221 ccaaccagcg gcatacagat tctgattttc tttttcaaat acttcctggt ctttttcagt
     2281 atgattgaat gttttaacaa tggtatgacc accatatgtt tcttctactt ggttattcaa
     2341 taaacctaaa cttttttgct gatctgcgaa aaacacttgt gatttcggcg ctacgatcat
     2401 cacgatgatc aagcttaaag ggatcatcaa taacgcgact aacgtcaatt gccagctgat
     2461 tgaaagcatc atcacgatga ccccaatcaa tgtgactgca ctagttacaa attgagtcaa
     2521 attttgctgc agcgtgcttg cgatgttatc catatcattg attgctcgag acatgatatc
     2581 cccatttgta tgtgtatcat aataggaaat cggtacacga ttcattttgg cttccaaatc
     2641 atgacgtaat tcataaactg ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa
     2701 gttgaataca gcagaaatca aatacatcac gattactgtc gcaatgatct gcgcgatttt
     2761 atcaaagtct atcggaaatt ttgttacttg ttggccttgt tgcatctgtt gagcgccagc
     2821 cattacacct ttgaatattt ctgtggttgc tttccccaag acctttggtg tttggatttg
     2881 gaagatcacg gctgctattg ctagtacaaa tacagcgatg atcgcaacca tacgtttaga
     2941 catatagcca aacaatcggc gtaccgttcc ccagaaattt ttaggctttt caccttttac
     3001 accgagattt ctgccaggtc caccatgtcc tggaccgcct gctggtccat gagagggacg
     3061 tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt gtgaatgaac gatttcttga
     3121 taagtttcgt ttgttgctaa tagttcttca tgtgttcctt tgccaaccag ttttccttca
     3181 tctagcacaa ggatcatatc tgcatccatt actgtactta ctcgttgagc aactaaaacg
     3241 gtgatacttt ctttcatatt cgttttcaat gcttggcgaa gattggcatc cgttttgaaa
     3301 tccaacgctg agaaggaatc atcaaacaca tagatatctg ctgttttgac taatgcccgt
     3361 gcaattgcca gacgctgtct ttgtccacca gagaagtttc ctcctccttg ttccacatgg
     3421 ctatctaagc catcttccat ctctgaaaca aaatctttag cttgtgcgat ttctaatgct
     3481 ttccaaatca tttcatctgt tgcatccggc ttaccatact gcatattgtc tcggatcgtt
     3541 ccagagaaca gaactgcttt ttgaggaaca aagcccatta attctcgcaa attatattga
     3601 gtcatgtcac taatgtccgt tccatttatt tggatcgaac cagattcgat gtcatataat
     3661 cgagggatca aattgactaa ggtcgtttta cctgaaccag tcccccctat gattgcgacg
     3721 atctcgcccg acttggcttg gaaatcaata tcttccaatg caagtttttc tgctccatga
     3781 tatcgataat tgacatgatt gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt
     3841 ttaggatttt cagggtcatg gataccgatt ttttcatcca acacttcatt aatacggtca
     3901 gctgaagctt gtgcacgagg caccatgata aaaatagcgg aaagcatcat aaagctgatc
     3961 aagatctgca tcgcataagt cataaatgca atcagattac cgacttccaa agtcatatct
     4021 gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg taccgctcat gatcaatgtc
     4081 atcattggca tcatcaatgc gacgatcgta ttaactttga ttgccgtatt tgtataatct
     4141 ttattcgcta aatcaaaacg attttcttca aagcgtgtct tatcgaatgc tcgaatcact
     4201 ctgacccctg tcaatccttc tcgaaatacc aggtttaaac ggtcagtttt cttttgcata
     4261 cttttgaata atggaacagc aaagtacatg attccgccga tcaaaataat cattactggg
     4321 atgacaacta agaagatttt tgtcaactga tgatcttttt gataagcaag tatgctcgcg
     4381 ccaatcaacg tgattggtgc attgatcatc atacgtaaaa acatttgtgt aaccatttgg
     4441 atttgagtca catcattggt cgttcttgtg atcaaagagg ccgttccata tttatcaaac
     4501 gcatcttttg tcagactctc tgatttttta tagacatccg tacgcaattg cttacccagt
     4561 ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag cagctaagat actgatcaaa
     4621 gaaaagccga tcatcacaaa acctgtatgc caaatataat cgacatctcc ttgcgctacc
     4681 cctttatcaa taatattgga ggtaagtgtc ggcaaatata aatctgccag tacttggatc
     4741 accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga tccgttttac gatttttagc
     4801 atctagttcc cttctttcta tttatatact ccgtttttca gccaagaaag tttcaaattg
     4861 aattcactga ttgcttgctc tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa
     4921 tgcgcaattg ttgtaaatac agtattcatc actaattgga tcagtaattc caattcatga
     4981 tcatttttta ctttcagctt cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt
     5041 ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt
     5101 cttgaggcac ggtaatccat gttcataaac aagttacgat aaaaagaagc attctcgcct
     5161 tccaaaatct cataaatcat tttagaaaaa taggattcga atcctttgaa tagatcacca
     5221 tcggattctt tcattagttg taacatatct ttagagctgt cacgtctcat tgtctcaaag
     5281 taataaaaat aaagatcctc tttatcttca aaatactgat agaaactgcc tctagggata
     5341 tccgctaatt tgacgatatt agcaatcgaa gcttctttta agggtactct agaaaactcg
     5401 attctcgcag cctcgattag tcttttttgt ttttctttag gcaaatggaa aaatgtctgt
     5461 gtcggcatat ggattctcct ttctttcaag atgacacatt gtcatatgac gctatgtcat
     5521 tataacacct cctacctgtt ttgcaagaca tttgcatagt ttttttcatt atcatccgat
     5581 acattaaaaa agtgataaga ggaatccttc catttgaatt aatgagagtt gcccattaaa
     5641 atagccacat actaaacaaa ggatgagata aatggcccaa tcaattagtg tccaagactt
     5701 caataaattg cctctcgatg ctgacagcat cgttttagat acaagaaata ccttagatta
     5761 cacaaaaaac cacttaagcc actcgctttc tatccccgct gctatcttgc caagacaatt
     5821 aaaaaatcta gatccaaaga aactttatta tattctaagc tatacaggca ggcgatcaga
     5881 agcattagcc gatcaactta ctgctaatgg ttttcgtgct gtttatatca ttggtgggat
     5941 ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt agattcaaaa gaggctgtga
     6001 caaaaaatcg tgtcacagcc tcttttatac cctgtctttt tttagttatt tattcttcaa
     6061 ccttatcaat atgacttgat agccttttct ttttcgagta aactgatttt gttagtcata
     6121 gttataacaa tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc
     6181 aaaggtcatc ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta
     6241 acaagttata aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat
     6301 gatattccgg aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca
     6361 gaattcactt ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact
     6421 gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc
     6481 ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat
     6541 acagaaaacg ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat
     6601 ggcggtgtat acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt
     6661 gtaggcatcg atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt
     6721 gcagatgagt tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa
     6781 aaattgagat aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgtg
     6841 ccttggatat tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt
     6901 cttcataaag agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag
     6961 ctgctgcata aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag
     7021 tcagcagaac taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg
     7081 gagagatcaa gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga
     7141 tcatgaatgg acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc
     7201 ctaagtaagg gatcaggttt aaaaaacccg caataatccc caacgtgaca gcatactcta
     7261 aaccaatgac cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt
     7321 gtcctcttat ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt
     7381 tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca
     7441 atagataaaa taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc
     7501 cgacaaaact tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg
     7561 ccgaattcat gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg
     7621 ggtcccgcaa aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac
     7681 tgatgaagct cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga
     7741 ttagtgcaac aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct
     7801 tttcaaagta atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag
     7861 gcagacctac gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa
     7921 ataaaaagac gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat
     7981 ttaaaaacca gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa
     8041 ttcctcctgt tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg
     8101 atgaagatag attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga
     8161 tacgtgagct actgtcttta aattcccatt ggccatcgga ccgacataat cgatcgtata
     8221 tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga
     8281 tacttcttga atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat
     8341 cagcatgggt tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat
     8401 cattgcactc tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc
     8461 cgtgtggaat aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga
     8521 atcagctcat atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg
     8581 aatctatacg atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt
     8641 tgaagaaaat agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac
     8701 tagtgtggat ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc
     8761 ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact
     8821 atacggggcg aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga
     8881 actgaaagca gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga
     8941 tcatgcgcat gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct
     9001 tggtacagat cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac
     9061 taccttcact gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca
     9121 attcgcttat ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga
     9181 aacaggtgct tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga
     9241 aaatggaggg gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg
     9301 aggacataac tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa
     9361 gcaaatcatc tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa
     9421 ttttgtcgtg tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga
     9481 aacaggatta ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta
     9541 ttttgatcgt tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact
     9601 acgggaagaa ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga
     9661 agaggaatat cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa
     9721 tgtagaaaac taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc
     9781 gtctatcctg atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga
     9841 aaaggagtat ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca
     9901 ttatcataaa ggattttgat cactctatca taaaggtaat attacattta tattagataa
     9961 ctctagtttt tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt
    10021 tttggatggc agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga
    10081 atagatcttc atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg
    10141 ctacatatga aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg
    10201 gacgcaaagt actgactttc ataagcattg cccgatattt ttttgttcct tcttcaaata
    10261 acaggatgat ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg
    10321 cgtttctgat cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga
    10381 cgatttgatc gatggactgc acataatagc caatattcgg taacatgttc gttttcctac
    10441 tttctccatt gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg
    10501 aaaaaattta atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct
    10561 gctaaactat attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa
    10621 atctcttgtt gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac
    10681 agttctcgtt caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca
    10741 tttgttgtct gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt
    10801 gcatcgtgag tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt
    10861 tcatgcaatg tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga
    10921 tgatcccccc aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac
    10981 tgcttcagtg ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc
    11041 ccacttccat ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc
    11101 atatgtgtct gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt
    11161 gtcattactt ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc
    11221 gtataggtca tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc
    11281 tctcttttat acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg
    11341 tttaacgctg aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg
    11401 gtggtgagtt gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata
    11461 tttgctgttc caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct
    11521 acttcataat aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactatga
    11581 tctgagaaac tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta
    11641 tttttttcat cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa
    11701 tatccttcag gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa
    11761 ccagtgttat agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat
    11821 gcttttcgca acaaattatc gggattgttg aaattactga tataccctag cagtccaatt
    11881 gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga
    11941 aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac
    12001 agcatagtgc cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct
    12061 ggataaatcg gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg
    12121 atggctacga catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa
    12181 ataagaataa tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa
    12241 gcaaatttca acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta
    12301 ctaaaaacaa caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc
    12361 tgtttggtcg aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa
    12421 taaaagaatg ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag
    12481 aagaattaat gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag
    12541 cggacctttc gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca
    12601 aaatcagtac tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat
    12661 atatctgcga tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg
    12721 gttttctttt gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat
    12781 ggataattga ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact
    12841 gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc cccctagatg aggcaacata
    12901 agtatacggt cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga
    12961 tgttgttttt ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca
    13021 ttgataagta cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt
    13081 gtatcttctg tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct
    13141 aaatctgctt gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc
    13201 atccctaagc tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga
    13261 ccgatggttt tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt
    13321 tctaaaatat taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg
    13381 gaagataaga gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt
    13441 gtattcatga cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca
    13501 acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta
    13561 ctacgaataa ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt
    13621 aacttttcta tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc
    13681 cctccttact aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact
    13741 tcctagaagc aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg
    13801 acaagttact gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga
    13861 taaatggtgt tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag
    13921 caaatttcgt caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt
    13981 tatctaataa ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt
    14041 gttagattta gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca
    14101 ccttcatgga aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct
    14161 tcagtcgttg gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa
    14221 tctgccatcg atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga
    14281 tcttctctat ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc
    14341 gtcggatcta cttccatcat atgggattcc aagattgctt cttggccact agctagctcg
    14401 taagtataat cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga
    14461 tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa
    14521 cgttgtacag ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta
    14581 aaagcgttat agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt
    14641 acttttacat gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat
    14701 tccttgtact cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt
    14761 acaagatctc cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact
    14821 ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt
    14881 ccaaaacttt cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc
    14941 tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg
    15001 aacccatatt cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc
    15061 gttttccaag gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga
    15121 agctttgtcc cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca
    15181 cctgctactt cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta
    15241 actgctaatt cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg
    15301 accatttctt gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt
    15361 gatcctacaa caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt
    15421 tatttgtttt gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa
    15481 atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg
    15541 catacttcat ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta
    15601 aatggaccgt gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct
    15661 ttgaatgttt ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt
    15721 tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc
    15781 atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg
    15841 acgattcctc cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa
    15901 gagggattga gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct
    15961 gctgtcatct cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga
    16021 tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga
    16081 tttgcttggt atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg
    16141 caattgatga atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat
    16201 ctgatagcct gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa
    16261 cacgtcttct gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc
    16321 taataacgcg attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc
    16381 ctccatagca gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg
    16441 ccccactaaa cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat
    16501 tgcaccaaaa gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc
    16561 tggtgtacgt acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa
    16621 ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa
    16681 taccgtgaat aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa
    16741 aatactgacc caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag
    16801 ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc
    16861 taatacgatc atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac
    16921 actgttcgta gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc
    16981 tggtctcaaa tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc
    17041 ttcttctcct gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc
    17101 tttttcgatt aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg
    17161 atcgatagga aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg
    17221 ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt
    17281 attcaaaata gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa
    17341 tttttccgtt aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg
    17401 tactagtaac tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc
    17461 aatcgacccg attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt
    17521 tcggtaactg acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt
    17581 ttctagtttg ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat
    17641 caatacagct ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat
    17701 gacatctaac atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg
    17761 tcaaacctga tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta
    17821 cgtacatgtc aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca
    17881 taagttataa aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa
    17941 ggagagaata cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg
    18001 cgcatttggc ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt
    18061 tttacagcat gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc
    18121 ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg
    18181 acgacgaaaa atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc
    18241 atctgcccct cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc
    18301 tgttggcgct gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact
    18361 aaggggaaga ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta
    18421 tgttgcagat tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg
    18481 gctcgctgct gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc
    18541 gcgttttctt atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg
    18601 gacaccaaaa gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa
    18661 gacaaccgga acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc
    18721 cggtattggt gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat
    18781 tccgctgatc gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat
    18841 catccaagga atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt
    18901 caatcggcga actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc
    18961 agctgtttta ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt
    19021 atgtatttat gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg
    19081 cgaaatattc ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg
    19141 gattggttct ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga
    19201 aatcgttttc ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat
    19261 cgtaccagaa actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac
    19321 ttatcctaaa cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct
    19381 tcaagaaata agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc
    19441 ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg
    19501 gagaggtggt gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc
    19561 gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc
    19621 tttcactgtt tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc
    19681 tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg
    19741 cgtcaatgtt gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac
    19801 gatcgatagg tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact
    19861 agctatttga tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg
    19921 tactgttttt tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt
    19981 caaaaaacgc acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc
    20041 ttgaagatcg tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata
    20101 ttctgctgcc ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc
    20161 ttcataagca gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag
    20221 tgctaagttt gggttatact gattactttt cgtaggctca acgatcaaac catctacccc
    20281 ataagcaatc attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc
    20341 taaaagcaat gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg
    20401 aaaaatataa tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg
    20461 tgttttttct ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag
    20521 atagccctca tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata
    20581 tatttcttgc agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag
    20641 gatcttttgt tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt
    20701 ttatttgtac gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg
    20761 tttcttgtgc tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat
    20821 tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt
    20881 ggttcttctc tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg
    20941 taaacgagtt gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct
    21001 ccgataattt atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca
    21061 ggcccatggc atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat
    21121 tgcgagtaag gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact
    21181 caggtataga aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata
    21241 gataaagatt agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct
    21301 ttccttgaga agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt
    21361 gtcccttttc tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca
    21421 tgcggtgatg atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat
    21481 accattttcc attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt
    21541 cttcatgagc ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg
    21601 cccttcttaa ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat
    21661 ctgtatgttt atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg
    21721 tcttcatcat aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt
    21781 ttctatccct acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt
    21841 tattttttct ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta
    21901 gtgctctcct tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt
    21961 tgtgcgattg atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac
    22021 ataaggaaca ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa
    22081 tacttgacct ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca
    22141 tgcttgtcct ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca
    22201 actaatcttt acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa
    22261 tgtatcttcc atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg
    22321 tcttttttcc ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc
    22381 acttctcaaa tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc
    22441 gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt
    22501 agtcaagtct tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt
    22561 ctcataagct tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt
    22621 agagcttcca actaagattg tttcaattga atcatccact aacttcatga cttttgaggc
    22681 ttcatttgct aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc
    22741 tagctcattc cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat
    22801 tcttaaatca ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc
    22861 ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact
    22921 gatccattta aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa
    22981 atcaattttt tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga
    23041 tgtaaagttt cctccaggat agcgaatgac acctaattct aattccttta cataagagag
    23101 gacatcttgt ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt
    23161 atagacgaca cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc
    23221 taccgtaaaa tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct
    23281 ttcatttcct atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa
    23341 aataaaagga gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag
    23401 aatatttctc aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa
    23461 tgtgatatct ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat
    23521 aagtaaaact acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt
    23581 aacaagaaaa taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg
    23641 ctttattcct tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc
    23701 cattacataa aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat
    23761 tacccagaga atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt
    23821 agtctcaact attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca
    23881 aaatatttct gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca
    23941 gccattacag gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt
    24001 aaagttctca tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta
    24061 ttcatcagcg tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt
    24121 ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct
    24181 ggtaaagtaa taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga
    24241 atgatcaaac ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga
    24301 atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat
    24361 tttcctttga aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa
    24421 gtgatcactg tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc
    24481 gttgtccaaa ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga
    24541 aatgagataa tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac
    24601 gcaataacca acaatataac tagcatacta atcgttcgaa tgattttttt accactaact
    24661 gcttttcgac tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc
    24721 ctatttcatt tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc
    24781 aacaatgata aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt
    24841 aaatcctcga ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc
    24901 ggttgcaaca tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca
    24961 aaaacctaag gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt
    25021 tgcaccatcc atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa
    25081 gagaatcgtc atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat
    25141 agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta
    25201 aggaatcgcc cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa
    25261 tgaacaaata actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt
    25321 agataatgct actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt
    25381 gaaatataaa gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa
    25441 attttcaaaa cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaacta
//
LOCUS       DACDHJ010000020        25320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDHJ010000020 DACDHJ010000000
VERSION     DACDHJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.020"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     regulatory      15..112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            228..809
                     /locus_tag="IV223_000662"
     CDS             228..809
                     /locus_tag="IV223_000662"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8710969.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            812..2119
                     /locus_tag="IV223_000663"
     CDS             812..2119
                     /locus_tag="IV223_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8710970.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2412..2900
                     /gene="purE"
                     /locus_tag="IV223_000664"
     CDS             2412..2900
                     /gene="purE"
                     /locus_tag="IV223_000664"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8710971.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2884..4017
                     /gene="purK"
                     /locus_tag="IV223_000665"
     CDS             2884..4017
                     /gene="purK"
                     /locus_tag="IV223_000665"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8710972.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4030..5325
                     /gene="purB"
                     /locus_tag="IV223_000666"
     CDS             4030..5325
                     /gene="purB"
                     /locus_tag="IV223_000666"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8710973.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5505..5882)
                     /locus_tag="IV223_000667"
     CDS             complement(5505..5882)
                     /locus_tag="IV223_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8710974.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6138..6866
                     /locus_tag="IV223_000668"
     CDS             6138..6866
                     /locus_tag="IV223_000668"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8710975.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6866..7120
                     /gene="purS"
                     /locus_tag="IV223_000669"
     CDS             6866..7120
                     /gene="purS"
                     /locus_tag="IV223_000669"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8710976.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7122..7793
                     /gene="purQ"
                     /locus_tag="IV223_000670"
     CDS             7122..7793
                     /gene="purQ"
                     /locus_tag="IV223_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8710977.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7794..10016
                     /gene="purL"
                     /locus_tag="IV223_000671"
     CDS             7794..10016
                     /gene="purL"
                     /locus_tag="IV223_000671"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8710978.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10001..11440
                     /locus_tag="IV223_000672"
     CDS             10001..11440
                     /locus_tag="IV223_000672"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8710979.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11472..12515
                     /locus_tag="IV223_000673"
     CDS             11472..12515
                     /locus_tag="IV223_000673"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8710980.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            12512..13090
                     /gene="purN"
                     /locus_tag="IV223_000674"
     CDS             12512..13090
                     /gene="purN"
                     /locus_tag="IV223_000674"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8710981.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13087..14622
                     /gene="purH"
                     /locus_tag="IV223_000675"
     CDS             13087..14622
                     /gene="purH"
                     /locus_tag="IV223_000675"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8710982.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14638..15912
                     /gene="purD"
                     /locus_tag="IV223_000676"
     CDS             14638..15912
                     /gene="purD"
                     /locus_tag="IV223_000676"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8710983.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16034..16948
                     /locus_tag="IV223_000677"
     CDS             16034..16948
                     /locus_tag="IV223_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8710984.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17081..18646)
                     /locus_tag="IV223_000678"
     CDS             complement(17081..18646)
                     /locus_tag="IV223_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8710985.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFKENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18639..20090)
                     /locus_tag="IV223_000679"
     CDS             complement(18639..20090)
                     /locus_tag="IV223_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8710986.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20104..21120)
                     /locus_tag="IV223_000680"
     CDS             complement(20104..21120)
                     /locus_tag="IV223_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8710987.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21340..22428
                     /locus_tag="IV223_000681"
     CDS             21340..22428
                     /locus_tag="IV223_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8710988.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22482..24008
                     /locus_tag="IV223_000682"
     CDS             22482..24008
                     /locus_tag="IV223_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8710989.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24010..25215
                     /locus_tag="IV223_000683"
     CDS             24010..25215
                     /locus_tag="IV223_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8710990.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         8044 a         4312 c         5797 g         7167 t
ORIGIN      
        1 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
       61 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
      121 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
      181 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
      241 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
      301 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
      361 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
      421 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
      481 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
      541 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
      601 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
      661 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
      721 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
      781 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
      841 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
      901 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
      961 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
     1021 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
     1081 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
     1141 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
     1201 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
     1261 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
     1321 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
     1381 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
     1441 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
     1501 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
     1561 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
     1621 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
     1681 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
     1741 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
     1801 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
     1861 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
     1921 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
     1981 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
     2041 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
     2101 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
     2161 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
     2221 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
     2281 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
     2341 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
     2401 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
     2461 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
     2521 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
     2581 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
     2641 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
     2701 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
     2761 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
     2821 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
     2881 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
     2941 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
     3001 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
     3061 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
     3121 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
     3181 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
     3241 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
     3301 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
     3361 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
     3421 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
     3481 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
     3541 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
     3601 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
     3661 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
     3721 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
     3781 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
     3841 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
     3901 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
     3961 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
     4021 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
     4081 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
     4141 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
     4201 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
     4261 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
     4321 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
     4381 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
     4441 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
     4501 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
     4561 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
     4621 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
     4681 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
     4741 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
     4801 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
     4861 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
     4921 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
     4981 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
     5041 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
     5101 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
     5161 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
     5221 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
     5281 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
     5341 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
     5401 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
     5461 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
     5521 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
     5581 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
     5641 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
     5701 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
     5761 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
     5821 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
     5881 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
     5941 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
     6001 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
     6061 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
     6121 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
     6181 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
     6241 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
     6301 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
     6361 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
     6421 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
     6481 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
     6541 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
     6601 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
     6661 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
     6721 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
     6781 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
     6841 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
     6901 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
     6961 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
     7021 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
     7081 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
     7141 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
     7201 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
     7261 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
     7321 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
     7381 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
     7441 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
     7501 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
     7561 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
     7621 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
     7681 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
     7741 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
     7801 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
     7861 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
     7921 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
     7981 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
     8041 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
     8101 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
     8161 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
     8221 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
     8281 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
     8341 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
     8401 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
     8461 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
     8521 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
     8581 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
     8641 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
     8701 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
     8761 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
     8821 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
     8881 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
     8941 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
     9001 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
     9061 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
     9121 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
     9181 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
     9241 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
     9301 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
     9361 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
     9421 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
     9481 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
     9541 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
     9601 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
     9661 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
     9721 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
     9781 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
     9841 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
     9901 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
     9961 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    10021 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    10081 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    10141 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    10201 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    10261 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    10321 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    10381 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    10441 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    10501 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    10561 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    10621 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    10681 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    10741 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    10801 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    10861 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    10921 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    10981 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    11041 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    11101 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    11161 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    11221 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    11281 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    11341 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    11401 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    11461 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    11521 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    11581 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    11641 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    11701 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    11761 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    11821 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    11881 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    11941 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    12001 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    12061 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    12121 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    12181 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    12241 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    12301 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    12361 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    12421 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    12481 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    12541 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    12601 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    12661 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    12721 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    12781 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    12841 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    12901 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    12961 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    13021 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    13081 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    13141 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    13201 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    13261 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    13321 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    13381 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    13441 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    13501 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    13561 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    13621 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    13681 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    13741 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    13801 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    13861 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    13921 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    13981 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    14041 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    14101 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    14161 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    14221 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    14281 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    14341 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    14401 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    14461 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    14521 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    14581 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    14641 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    14701 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    14761 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    14821 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    14881 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    14941 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    15001 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    15061 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    15121 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    15181 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    15241 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    15301 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    15361 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    15421 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    15481 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    15541 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    15601 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    15661 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    15721 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    15781 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    15841 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    15901 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    15961 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    16021 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    16081 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    16141 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    16201 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    16261 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    16321 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    16381 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    16441 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    16501 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    16561 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    16621 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    16681 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    16741 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    16801 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    16861 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    16921 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    16981 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    17041 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    17101 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    17161 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    17221 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    17281 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    17341 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    17401 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    17461 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    17521 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    17581 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    17641 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    17701 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    17761 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    17821 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    17881 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    17941 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    18001 aaacagcaga gaagatcact tgttgattct ccttaaataa aaacttcaaa taatcactga
    18061 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    18121 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    18181 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    18241 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    18301 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    18361 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    18421 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    18481 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    18541 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    18601 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    18661 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    18721 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    18781 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    18841 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    18901 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    18961 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    19021 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    19081 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    19141 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    19201 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    19261 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    19321 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    19381 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    19441 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    19501 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    19561 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    19621 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    19681 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    19741 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    19801 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    19861 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    19921 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    19981 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    20041 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    20101 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    20161 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    20221 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    20281 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    20341 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    20401 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    20461 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    20521 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    20581 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    20641 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    20701 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    20761 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    20821 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    20881 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    20941 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    21001 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    21061 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    21121 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    21181 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    21241 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    21301 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    21361 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    21421 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    21481 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    21541 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    21601 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    21661 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    21721 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    21781 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    21841 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    21901 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    21961 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    22021 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    22081 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    22141 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    22201 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    22261 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    22321 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    22381 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    22441 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    22501 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    22561 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    22621 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    22681 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    22741 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    22801 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    22861 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    22921 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    22981 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    23041 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    23101 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    23161 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    23221 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    23281 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    23341 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    23401 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    23461 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    23521 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    23581 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    23641 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    23701 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    23761 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    23821 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    23881 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    23941 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    24001 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    24061 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    24121 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    24181 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    24241 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    24301 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    24361 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    24421 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    24481 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    24541 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    24601 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    24661 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    24721 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    24781 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    24841 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    24901 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    24961 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    25021 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    25081 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    25141 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    25201 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    25261 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
//
LOCUS       DACDHJ010000021        25136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDHJ010000021 DACDHJ010000000
VERSION     DACDHJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.021"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            59..3337
                     /locus_tag="IV223_000684"
     CDS             59..3337
                     /locus_tag="IV223_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8710991.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3878..5785)
                     /locus_tag="IV223_000685"
     CDS             complement(3878..5785)
                     /locus_tag="IV223_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8710992.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5915..6790
                     /locus_tag="IV223_000686"
     CDS             5915..6790
                     /locus_tag="IV223_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8710993.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6870..7844
                     /locus_tag="IV223_000687"
     CDS             6870..7844
                     /locus_tag="IV223_000687"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8710994.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8033..9169
                     /locus_tag="IV223_000688"
     CDS             8033..9169
                     /locus_tag="IV223_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8710995.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9306..9650
                     /locus_tag="IV223_000689"
     CDS             9306..9650
                     /locus_tag="IV223_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8710996.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9921..11207
                     /gene="efmA"
                     /locus_tag="IV223_000690"
     CDS             9921..11207
                     /gene="efmA"
                     /locus_tag="IV223_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8710997.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11345..11944)
                     /locus_tag="IV223_000691"
     CDS             complement(11345..11944)
                     /locus_tag="IV223_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8710998.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12348..13469
                     /gene="mnmA"
                     /locus_tag="IV223_000692"
     CDS             12348..13469
                     /gene="mnmA"
                     /locus_tag="IV223_000692"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8710999.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13715..15136
                     /gene="arcD"
                     /locus_tag="IV223_000693"
     CDS             13715..15136
                     /gene="arcD"
                     /locus_tag="IV223_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8711000.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15150..16268
                     /locus_tag="IV223_000694"
     CDS             15150..16268
                     /locus_tag="IV223_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8711001.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16328..17620)
                     /locus_tag="IV223_000695"
     CDS             complement(16328..17620)
                     /locus_tag="IV223_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8711002.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17770..18330
                     /locus_tag="IV223_000696"
     CDS             17770..18330
                     /locus_tag="IV223_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8711003.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18459..18836
                     /locus_tag="IV223_000697"
     CDS             18459..18836
                     /locus_tag="IV223_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8711004.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18769..20841
                     /locus_tag="IV223_000698"
     CDS             18769..20841
                     /locus_tag="IV223_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8711005.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21052..21999)
                     /locus_tag="IV223_000699"
     CDS             complement(21052..21999)
                     /locus_tag="IV223_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8711006.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22245..22709
                     /locus_tag="IV223_000700"
     CDS             22245..22709
                     /locus_tag="IV223_000700"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8711007.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22771..23814)
                     /locus_tag="IV223_000701"
     CDS             complement(22771..23814)
                     /locus_tag="IV223_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8711008.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24191..>25136)
                     /locus_tag="IV223_000702"
     CDS             complement(24191..>25136)
                     /locus_tag="IV223_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8711009.1"
                     /translation="IPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIFIFV
                     KLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVIFQV
                     ILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISLICY
                     AFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALFALS
                     QVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLDFLK
                     VAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         8223 a         4602 c         5284 g         7027 t
ORIGIN      
        1 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
       61 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      121 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      181 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      241 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      301 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      361 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      421 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      481 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      541 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      601 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      661 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      721 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      781 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      841 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      901 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
      961 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1021 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1081 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1141 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1201 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1261 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1321 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1381 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1441 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1501 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1561 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1621 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1681 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1741 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1801 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1861 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1921 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     1981 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2041 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2101 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2161 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2221 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2281 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2341 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2401 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2461 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2521 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2581 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2641 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2701 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2761 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2821 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2881 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     2941 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3001 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3061 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3121 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3181 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3241 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3301 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3361 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3421 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3481 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3541 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3601 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3661 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3721 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3781 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3841 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3901 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     3961 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4021 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4081 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4141 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4201 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4261 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4321 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4381 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4441 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4501 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4561 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4621 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4681 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4741 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4801 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4861 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4921 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     4981 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5041 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5101 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5161 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5221 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5281 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5341 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5401 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5461 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5521 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5581 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5641 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5701 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5761 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5821 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5881 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     5941 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6001 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6061 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6121 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6181 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6241 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6301 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6361 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6421 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6481 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6541 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6601 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6661 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6721 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6781 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6841 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6901 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     6961 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7021 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7081 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7141 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7201 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7261 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7321 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7381 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7441 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7501 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7561 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7621 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7681 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7741 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7801 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7861 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7921 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     7981 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8041 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8101 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8161 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8221 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8281 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8341 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8401 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8461 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8521 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8581 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8641 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8701 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8761 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8821 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8881 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     8941 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9001 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9061 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9121 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9181 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9241 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9301 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9361 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9421 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9481 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9541 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9601 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9661 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9721 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9781 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9841 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     9901 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     9961 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10021 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10081 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10141 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10201 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10261 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10321 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10381 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10441 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10501 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10561 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10621 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10681 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10741 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10801 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10861 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    10921 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    10981 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11041 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11101 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11161 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
    11221 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11281 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11341 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11401 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
    11461 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11521 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11581 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11641 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11701 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11761 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11821 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    11881 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
    11941 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12001 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
    12061 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12121 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12181 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12241 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12301 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12361 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12421 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12481 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12541 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12601 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12661 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12721 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12781 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12841 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    12901 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
    12961 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13021 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13081 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13141 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13201 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13261 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13321 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13381 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13441 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13501 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13561 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13621 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
    13681 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
    13741 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13801 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13861 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
    13921 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    13981 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14041 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
    14101 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14161 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14221 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14281 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14341 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14401 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14461 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14521 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14581 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14641 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14701 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14761 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14821 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    14881 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
    14941 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15001 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15061 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15121 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15181 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15241 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
    15301 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15361 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15421 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15481 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15541 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15601 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15661 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15721 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15781 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15841 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    15901 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    15961 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16021 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16081 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16141 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16201 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16261 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16321 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
    16381 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16441 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
    16501 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
    16561 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
    16621 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
    16681 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16741 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16801 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
    16861 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    16921 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    16981 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17041 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17101 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17161 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17221 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17281 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17341 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
    17401 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17461 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17521 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17581 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17641 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
    17701 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17761 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17821 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    17881 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    17941 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    18001 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18061 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    18121 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    18181 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    18241 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    18301 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    18361 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18421 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18481 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    18541 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18601 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18661 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    18721 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    18781 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18841 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    18901 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    18961 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19021 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19081 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19141 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19201 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19261 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19321 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19381 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19441 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19501 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19561 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19621 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19681 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19741 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19801 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19861 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    19921 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    19981 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20041 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    20101 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    20161 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20221 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    20281 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20341 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20401 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20461 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20521 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20581 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20641 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20701 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20761 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20821 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    20881 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    20941 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    21001 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    21061 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    21121 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    21181 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    21241 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    21301 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    21361 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    21421 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    21481 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    21541 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    21601 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    21661 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    21721 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    21781 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    21841 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    21901 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    21961 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    22021 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    22081 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    22141 tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag
    22201 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    22261 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    22321 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    22381 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    22441 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    22501 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    22561 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    22621 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    22681 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    22741 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    22801 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    22861 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    22921 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    22981 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    23041 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    23101 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    23161 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    23221 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    23281 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    23341 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    23401 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    23461 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    23521 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    23581 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    23641 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    23701 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    23761 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    23821 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    23881 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    23941 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    24001 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    24061 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    24121 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    24181 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    24241 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    24301 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    24361 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    24421 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    24481 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    24541 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    24601 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    24661 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    24721 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    24781 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    24841 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    24901 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    24961 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    25021 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    25081 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaata
//
LOCUS       DACDHJ010000022        24621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDHJ010000022 DACDHJ010000000
VERSION     DACDHJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.022"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(58..186)
                     /locus_tag="IV223_000703"
     CDS             complement(58..186)
                     /locus_tag="IV223_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8711010.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="IV223_000704"
     CDS             complement(189..1562)
                     /gene="rlmD"
                     /locus_tag="IV223_000704"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8711011.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1673..2728)
                     /locus_tag="IV223_000705"
     CDS             complement(1673..2728)
                     /locus_tag="IV223_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8711012.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="IV223_000706"
     CDS             complement(2738..4168)
                     /gene="gatB"
                     /locus_tag="IV223_000706"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8711013.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="IV223_000707"
     CDS             complement(4165..5637)
                     /gene="gatA"
                     /locus_tag="IV223_000707"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8711014.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="IV223_000708"
     CDS             complement(5637..5942)
                     /gene="gatC"
                     /locus_tag="IV223_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8711015.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="IV223_000709"
     CDS             complement(6048..8084)
                     /gene="ligA"
                     /locus_tag="IV223_000709"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8711016.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="IV223_000710"
     CDS             complement(8101..10335)
                     /gene="pcrA"
                     /locus_tag="IV223_000710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8711017.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10490..12049
                     /locus_tag="IV223_000711"
     CDS             10490..12049
                     /locus_tag="IV223_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8711018.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12160..14091)
                     /locus_tag="IV223_000712"
     CDS             complement(12160..14091)
                     /locus_tag="IV223_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8711019.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="IV223_000713"
     CDS             complement(14109..15023)
                     /gene="pfkB"
                     /locus_tag="IV223_000713"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8711020.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15020..15772)
                     /locus_tag="IV223_000714"
     CDS             complement(15020..15772)
                     /locus_tag="IV223_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8711021.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15931..16140)
                     /locus_tag="IV223_000715"
     CDS             complement(15931..16140)
                     /locus_tag="IV223_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711022.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16151..17479)
                     /locus_tag="IV223_000716"
     CDS             complement(16151..17479)
                     /locus_tag="IV223_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711023.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17844..18431)
                     /locus_tag="IV223_000717"
     CDS             complement(17844..18431)
                     /locus_tag="IV223_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8711024.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="IV223_000718"
     CDS             complement(18514..19764)
                     /gene="clpX"
                     /locus_tag="IV223_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8711025.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20011..21300)
                     /locus_tag="IV223_000719"
     CDS             complement(20011..21300)
                     /locus_tag="IV223_000719"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8711026.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21454..22386)
                     /locus_tag="IV223_000720"
     CDS             complement(21454..22386)
                     /locus_tag="IV223_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711027.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22543..23202)
                     /locus_tag="IV223_000721"
     CDS             complement(22543..23202)
                     /locus_tag="IV223_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8711028.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="IV223_000722"
     CDS             complement(23205..24230)
                     /gene="sppA"
                     /locus_tag="IV223_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8711029.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24243..24533)
                     /locus_tag="IV223_000723"
     CDS             complement(24243..24533)
                     /locus_tag="IV223_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711030.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
BASE COUNT         6545 a         5329 c         4330 g         8417 t
ORIGIN      
        1 aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt agcacgtcta
       61 tttattaaag attttagggc cttttctcat gtttttacga tcattcttag gtgtttccat
      121 ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt tttctttttg
      181 gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg
      241 aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa taacttagca
      301 tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg tgacatttcg
      361 attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac tacgatcaca
      421 gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt tccgacttca
      481 taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc aattgcttct
      541 ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa gccaatcgta
      601 cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc aaactcgatg
      661 gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga ctttgctgag
      721 attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata taaaactttt
      781 tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat gaccgaaacc
      841 acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa gaatttttct
      901 ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg gataatgatg
      961 tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa ccgttgcaag
     1021 atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata aaaatcttcg
     1081 atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg tccatcgata
     1141 cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat tccgaccgtt
     1201 ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc tacttgtttt
     1261 tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat acctatgcga
     1321 agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa tgtttcgact
     1381 tttccatatc caaacttatt gcctgctttt actactagta tttctacttc ttctccaggg
     1441 agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat tccttcataa
     1501 ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg ctgttctgtc
     1561 atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata aatcagctaa
     1621 ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt gattaattct
     1681 tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca tccgttaaac
     1741 gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca tctgtacctg
     1801 tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat
     1861 tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc attactggtc
     1921 gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag attattcttg
     1981 gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag atctcaaaga
     2041 tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg gcatctggcg
     2101 cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc gaagcatttc
     2161 cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact cgagggagca
     2221 tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt tctgatggaa
     2281 cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag taactgttat
     2341 cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct gctgctgctt
     2401 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
     2461 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
     2521 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
     2581 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
     2641 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
     2701 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
     2761 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
     2821 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
     2881 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
     2941 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
     3001 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
     3061 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
     3121 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
     3181 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
     3241 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
     3301 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
     3361 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
     3421 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
     3481 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
     3541 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
     3601 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
     3661 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
     3721 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
     3781 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
     3841 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
     3901 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
     3961 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
     4021 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
     4081 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
     4141 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
     4201 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
     4261 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
     4321 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
     4381 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
     4441 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
     4501 ttgtcccgct tttttgaagt aagcgtcata ataaccagca cttaaggaga atgtacctaa
     4561 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
     4621 atctaatgat ttcacatttt ctgagcgata accgtagcgg ataccatcga atcgttgcaa
     4681 gttggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
     4741 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
     4801 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
     4861 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
     4921 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
     4981 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
     5041 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
     5101 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
     5161 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
     5221 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
     5281 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
     5341 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
     5401 atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc
     5461 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cttcgacctc
     5521 aggctctgtt tctttgattc gttcaaatgt tgcttttgtc aaatcttgta cagtgatttc
     5581 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
     5641 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
     5701 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
     5761 acgtttactg tttcaaccac attcgacgta aatggaacac cttctgtatc tacttcttcc
     5821 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
     5881 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
     5941 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
     6001 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
     6061 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
     6121 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
     6181 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
     6241 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
     6301 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
     6361 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
     6421 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
     6481 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
     6541 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
     6601 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
     6661 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
     6721 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
     6781 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
     6841 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
     6901 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
     6961 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
     7021 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
     7081 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
     7141 aacttgtaag ctgctgccca tctcggtgct ttgacagtaa aaccaagttg atcttgtagc
     7201 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
     7261 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
     7321 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
     7381 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
     7441 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
     7501 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
     7561 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
     7621 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
     7681 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
     7741 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
     7801 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
     7861 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
     7921 tattgttcct caattgctac taattctgcg tattctttat cataaacata gtcttccact
     7981 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
     8041 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
     8101 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
     8161 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
     8221 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
     8281 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
     8341 gaatacaggt gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
     8401 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
     8461 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
     8521 gcgttcttct tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
     8581 ttctagacca atcaggaata caactgggaa ttctaatcct ttggctgcat gcaaagtcat
     8641 caatgtcact tgggaagcat cttcttctaa attgtcaata tcggaaacaa gtgccagatc
     8701 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
     8761 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
     8821 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
     8881 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
     8941 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
     9001 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
     9061 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
     9121 ttgaggattt gccagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
     9181 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
     9241 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
     9301 gcttcgaatc tcttcttgca tccgatctac gataaaacgg gtttcgtctc gttcattatc
     9361 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
     9421 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
     9481 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
     9541 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
     9601 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
     9661 ttggtactca tctacatgaa tataatggaa tttgttttga taatatgtga gtgaatcagg
     9721 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
     9781 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
     9841 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
     9901 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
     9961 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
    10021 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
    10081 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
    10141 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
    10201 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
    10261 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
    10321 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
    10381 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
    10441 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
    10501 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
    10561 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
    10621 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
    10681 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
    10741 caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat caatgaactg
    10801 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
    10861 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
    10921 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
    10981 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
    11041 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
    11101 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
    11161 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
    11221 tatttatcag aaggtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
    11281 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
    11341 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
    11401 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggtgg
    11461 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
    11521 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
    11581 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
    11641 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
    11701 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
    11761 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
    11821 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
    11881 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
    11941 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
    12001 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
    12061 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
    12121 agaagagact gggaaaaacc agcctcttct tttttatttc tatgcttctg ctgctgcagg
    12181 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
    12241 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccat gaggagccag
    12301 taatttgatc ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
    12361 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    12421 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    12481 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    12541 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    12601 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    12661 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    12721 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    12781 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    12841 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    12901 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    12961 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    13021 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    13081 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    13141 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    13201 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    13261 tactggtgca gtaccactaa gggctagatt gatcagctct tctggtttac ggataccatc
    13321 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    13381 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    13441 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    13501 cagagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    13561 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    13621 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    13681 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    13741 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    13801 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    13861 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    13921 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    13981 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    14041 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    14101 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    14161 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    14221 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccggcaat cattgaatcc
    14281 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    14341 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    14401 gctagcaatt ttttaccata cggaatgatt tcttctacag attggaaggt tgtattgtaa
    14461 agctcagcta attcgtgatt gtttggtttg atcaacaatg gtttcttaac aagtgcattt
    14521 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    14581 atcagttctt gataaaatcc ttttcgcagg ctagccggcg cgcttcctga caatacgacg
    14641 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    14701 atagatgggc ccaaaccgtt aatttcagtt tcagtatctg attttagttt gatattgatt
    14761 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    14821 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    14881 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    14941 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    15001 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    15061 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    15121 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    15181 aatttttcat ggctaaacgt ttaacagctg cctcttctgg gtccggagtc gtgaatcctt
    15241 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    15301 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    15361 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    15421 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    15481 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    15541 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    15601 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    15661 gtcttatcgt tgattcagaa gcattaaaaa ggtttacaag atcttgtgat ttcacgattt
    15721 tctgctgttc aagcaagtct aggatttttt gtctacgttc ctctgtaagc attttcacga
    15781 tcacctcaat acaaataata gcatattttt tttctactcg caatataaat cagtcaaaaa
    15841 cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaaa aagactggtt
    15901 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    15961 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    16021 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    16081 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    16141 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    16201 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    16261 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    16321 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    16381 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    16441 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgcgag cgattctcgt
    16501 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    16561 ctccttgttg aatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    16621 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    16681 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    16741 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    16801 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    16861 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    16921 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    16981 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    17041 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    17101 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    17161 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    17221 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    17281 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    17341 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    17401 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    17461 atttatcttc gccaatcatt ttctcacctc tttaaaatct ttttataatt taattataac
    17521 caacgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    17581 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    17641 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    17701 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    17761 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    17821 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    17881 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    17941 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    18001 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    18061 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg
    18121 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    18181 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    18241 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    18301 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    18361 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    18421 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    18481 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    18541 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    18601 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    18661 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    18721 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    18781 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    18841 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    18901 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    18961 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    19021 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    19081 tttacgtcct ccctgaggtg gcacgcttgc aacagttcct tctagaattt tcagcaaggc
    19141 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    19201 aattttatcg atctcatcaa tatagatgat ccctttttct gcacgttcca cattgtaatc
    19261 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    19321 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    19381 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    19441 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    19501 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    19561 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    19621 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    19681 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    19741 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    19801 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    19861 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    19921 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    19981 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    20041 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    20101 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    20161 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    20221 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    20281 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    20341 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    20401 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    20461 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    20521 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    20581 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    20641 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    20701 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    20761 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    20821 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    20881 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    20941 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    21001 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    21061 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    21121 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    21181 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    21241 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    21301 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt
    21361 taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt
    21421 tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa
    21481 aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt
    21541 ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt
    21601 atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttcattta acacggaact
    21661 aagcagcgca tcttcattac cgtaacgatc tgaaaataca cataattgct ggtaacttgc
    21721 ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt
    21781 caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct
    21841 tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg
    21901 ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg
    21961 aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa
    22021 ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt
    22081 acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata
    22141 aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa
    22201 tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa
    22261 gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt
    22321 tgctagtgct tgttgtgcta ctcgcattaa attctcatat ttttctggaa aatcaatcgg
    22381 cttcacgcag tgtcctcctc ttgctcatgt tcagctcatg ttcaatagta gcaaaacaaa
    22441 aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac
    22501 ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg
    22561 gttgaatgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcctag
    22621 atgttgcttt ttctgattaa agattgctgg aagataccct aacatcagca aaggaaattt
    22681 tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc
    22741 atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttgac catgattgaa
    22801 tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagatact
    22861 cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg
    22921 cgtaatagct ccgatacaca aaagatcgac caagaaagca aacattcgga taaaaaaacc
    22981 tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata
    23041 ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa
    23101 agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt
    23161 atctttttct tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa
    23221 taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga
    23281 ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta
    23341 gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc
    23401 tttcgcatag cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct
    23461 tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat
    23521 ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct
    23581 tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac
    23641 gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc
    23701 aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta
    23761 tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac
    23821 attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg
    23881 tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct
    23941 tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa
    24001 cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta
    24061 ctgtctccct cttccaacac atttgaagct agttcttcat ccccatataa ccaactgttc
    24121 aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc
    24181 acaaataatc ctgcagcaat cagaactgct gcccaacgtc tcttattcat tctttctcct
    24241 ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc
    24301 tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgtcaattc atcttctacc
    24361 gtaaactcac gagcttcagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc
    24421 cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg
    24481 gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact
    24541 cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa
    24601 cccattattt tttgacataa a
//
LOCUS       DACDHJ010000023        23917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDHJ010000023 DACDHJ010000000
VERSION     DACDHJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.023"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(289..459)
                     /locus_tag="IV223_000724"
     CDS             complement(289..459)
                     /locus_tag="IV223_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8711031.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(653..832)
                     /locus_tag="IV223_000725"
     CDS             complement(653..832)
                     /locus_tag="IV223_000725"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711032.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1219..2331
                     /locus_tag="IV223_000726"
     CDS             1219..2331
                     /locus_tag="IV223_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8711033.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2409..2702)
                     /locus_tag="IV223_000727"
     CDS             complement(2409..2702)
                     /locus_tag="IV223_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8711034.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            2870..3184
                     /locus_tag="IV223_000728"
     CDS             2870..3184
                     /locus_tag="IV223_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8711035.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3205..5184
                     /locus_tag="IV223_000729"
     CDS             3205..5184
                     /locus_tag="IV223_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8711036.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5197..5523
                     /locus_tag="IV223_000730"
     CDS             5197..5523
                     /locus_tag="IV223_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8711037.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5539..6057
                     /locus_tag="IV223_000731"
     CDS             5539..6057
                     /locus_tag="IV223_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711038.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6057..7409
                     /gene="celB"
                     /locus_tag="IV223_000732"
     CDS             6057..7409
                     /gene="celB"
                     /locus_tag="IV223_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8711039.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7493..8998)
                     /locus_tag="IV223_000733"
     CDS             complement(7493..8998)
                     /locus_tag="IV223_000733"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8711040.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9090..9680)
                     /locus_tag="IV223_000734"
     CDS             complement(9090..9680)
                     /locus_tag="IV223_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8711041.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            9859..11505
                     /locus_tag="IV223_000735"
     CDS             9859..11505
                     /locus_tag="IV223_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8711042.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11554..12273
                     /locus_tag="IV223_000736"
     CDS             11554..12273
                     /locus_tag="IV223_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8711043.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            12752..13699
                     /locus_tag="IV223_000737"
     CDS             12752..13699
                     /locus_tag="IV223_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8711044.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            13711..14631
                     /locus_tag="IV223_000738"
     CDS             13711..14631
                     /locus_tag="IV223_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8711045.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            14672..16129
                     /locus_tag="IV223_000739"
     CDS             14672..16129
                     /locus_tag="IV223_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8711046.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16474..17088
                     /locus_tag="IV223_000740"
     CDS             16474..17088
                     /locus_tag="IV223_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8711047.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17081..18805
                     /locus_tag="IV223_000741"
     CDS             17081..18805
                     /locus_tag="IV223_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8711048.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            18802..20253
                     /locus_tag="IV223_000742"
     CDS             18802..20253
                     /locus_tag="IV223_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8711049.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(20726..22879)
                     /locus_tag="IV223_000743"
     CDS             complement(20726..22879)
                     /locus_tag="IV223_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8711050.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(22881..>23917)
                     /locus_tag="IV223_000744"
     CDS             complement(22881..>23917)
                     /locus_tag="IV223_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8711051.1"
                     /translation="LYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRITSKRA
                     LTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFSKGLN
                     PVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKEFPVV
                     ILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRYLGYL
                     EALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQHGYHI
                     PEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSKFPVS
                     FIKRQSTKEK"
BASE COUNT         7590 a         4144 c         4771 g         7412 t
ORIGIN      
        1 aaataagtaa agacaatttt gtgctgcgtt gagaagtgaa aaataaacct ttgtagtaat
       61 actagttaat aaaaaatata caaaaacact gcgaaaatca ttcttgagtt tcgcagtgtt
      121 ttttgcctaa tgactcaagt ttattgggta atactttgat tttctttcta caaataatca
      181 gcgatcagtt gtaatttttt ctctagtttt aatggctctt tgtcaataag gagaaaaaca
      241 agtcgggtct tttgtctcgg aaagcttcaa ccaactcgtg aataaaggtt acgcctcctt
      301 gaaaggcgtg gaaaaaccaa gtaatcgatc aatcaccaat ttcgcatttg gtagaacatg
      361 tttggtgagc tgaaagtagg tggcgttcat gtctgtaact aagaattcta tttctttagg
      421 attagtgcag ctaaggaaat agtttgttaa tcgaggaatt ttttgtgtag gtaaattttc
      481 tcgattaatt tttctgtctc gccatccgcg gaaataaaac tcagtttatc ctcaacggaa
      541 acgtgagaac gaaattcaca accctcaaca ctcaagacag aattcttttg gaagactttg
      601 gtaaatagct tctcaactct ttcaatgtgc gaataacggt tgtcaaagat acctaacaac
      661 ttttcgaaat aaaaaataac gacacttttt cagtcagtaa agaagtgatt ttatgtctga
      721 catgatttga aatcgagtgc cttggacgga taaaataact ttgagcgttc caatggcttc
      781 gacagttttt acaggtatag cgctgctttt ttaggcgtat aaccattggc atatgattat
      841 attattcaaa gtggacaatg gtttccttct tcttgttttt caccaccatt aatttcctat
      901 ttccatctac cacagcagaa ccacaatttc tacagacaca tggagcaggg aagagaactg
      961 cagcaacgat caatgttttt ttcttctgta aggtttcgta agagacctct gtaatcatca
     1021 aatttttgtc tattgatctc agaatttttt tgatagaacc attcataaag catatcgttc
     1081 tctcagttgt ttattttgtg gtgatttaat catacaagaa aacgatatgt ttttttataa
     1141 aaacatgaat aggaaaaata gttgacaaga aaatgacaac gccatatgat gttccatagt
     1201 atttacaacc tgtcatagat gactgtaaaa aataaaagta aaaagaaagg acgacaacag
     1261 gtggattttt atcagtctct acaactagat ccgtttattt taaaacaaaa aatacgagaa
     1321 gcagcttcta aaaaagaaaa gtgcatgtat atttgttcat tgtttttacg atcattattc
     1381 atcgtattgt ttgccatttg ttttatcatt ataattacaa cattatttga atcgaagcat
     1441 aaaccatacg ctgtggtttt attctgtatg ttgatgagta ttcgttttgt tgactttggt
     1501 tataagattt cccattcgat cataggttta gcaatcgtca tgttctcact attagtagca
     1561 ccgtatgtcc agctgatcaa atggtcagtt atgggcatgt tgattcattt tattttacta
     1621 agcagcatat taatggcaac cgctagtgat cccaaaatgg ggaatgcttc tttgtacggc
     1681 ttttcatatc tctttattgt ttattcgtta cctaaagatt cgttgaacaa gaatttcttt
     1741 acacaaacaa gtagcttatt atttttgttt ttttgttggt tttcagttct cctttacaga
     1801 aaacatcgag aaaagaacaa agacaaaagc ttgtttaaag agattttttt gaaaggcatg
     1861 tattcgcagg aaaaaatttg gatgctcatc tatgcatttg gcattagtct tttgatcgtt
     1921 gcaggagagt atgtcccttt tcagcgattg atgtgggcag ggtttgcatt ttcttctatt
     1981 gtatctagct atggattgat gtcgattggt ttcaaagaac gagcagtaga ccgaatcatt
     2041 ggctcattga ttggatgtgt tttatttatt ggtatcagcc aatttattcc ttttaactgg
     2101 gtcggtatat taggtggtct agctttaggt gtttgttcta cctatagata taaaacagtt
     2161 tttaattctt ttggtgcttt ggcaatcact gccagtttat tcggggttcc aggagcagta
     2221 acgatccgca tttttgagaa tatccttggg gtatgtttag gaatcatgta tattggggtg
     2281 actgaaatac ttatccgaaa aataagagaa aaacacggtt tgaatcatta gatgagatcg
     2341 aataaaaaat aaggaaaaag tgaagataca catggatgat aaattgctat cattccatgt
     2401 gtatcttttt acggtaaatc agtgaaacgt tcgactttca tttttaattt atacttttta
     2461 cggagttcgt ttatttgagc catcatttcg gatttgtgat gcttttcgat tgcttcttca
     2521 tctttccaac ggtcgatcag taaaagagat tctggatctt ctagtggaaa gtaatagtca
     2581 tatttctcgt ttccttcttc tgcacgaatc gctttgacaa tgtttctttc gaccatttct
     2641 tctgcaaatt ttctggcttg tccatttgta cttgtataca aaatattcac tgtgatactc
     2701 actaaatccc ctcctgaaaa aattctatca ctagtatatc acggtgccag aaattattat
     2761 tcaagatatg atacacattt tttccgcaat gctacggaaa tatatatttt gaatagaaaa
     2821 cgcttacaag atatgcttgg tttataaaaa taaaaggagt gattgattta tgaaaaaagc
     2881 attgattatt tgtgcggcag gaatgtcatc ctcgatgatg gcctcaaaga caacggattt
     2941 ctttaagcaa aaaagagaag aggtactcgt agatgcagtt tcagctacag aaggcgataa
     3001 tatgattaag aatagtgatt ttgatttatt tctgatcagc ccgcaaacga cgatgttttt
     3061 ggataaattc aaaaaattag gagaatctgt tggaaagcct gttgttagta tccctttcca
     3121 agcgtatgtc ccaatcccta caggcatcca gaagttagca gaggtgatcg aagagaacat
     3181 atgatggaat aggagtggat gataatgaaa cagaaggaaa aattgctgtt gagatatttg
     3241 attgatcaca agaaggagta tgtcaccagt caacgtttag cttctgaatt gttattatct
     3301 gatcggacaa tccgtaatta cttgcagcgg atcaaagagt tagtagaaaa aaacggaggt
     3361 aagatcattg ctaaaccagg ttatggctac caactgcata ttttacaaag actgacattt
     3421 gacttgtttt tgagtcggca agaaatagta cctggatatg ctagagaacc gcaaggattt
     3481 tatgagtccg aagatcgaca aaagtatata ttgaaccaat tgcttttgga agatgccatt
     3541 ctttttatgg atgatttggc agaagagctt tatatcagtc gttccagttt gacaaaggac
     3601 atgcaagaaa tcaaggagcg attgattcca tattcactaa aaatcgtttc taagcatggt
     3661 caagggacat ggatcgaagg ggaagaaaga aatcgacggc attttatcat ggatactttt
     3721 tttggaaacc attatggaaa ttcattgaaa gaatatttag ggcaaagccg ttttttccca
     3781 gacatcagtt ttgaagagct agtcattatc attctcgatg aaacaagaga agctaagtta
     3841 aaagtttcgg attttatcat acagaatctg actttacact tggctttggg gatcaaacgg
     3901 ctccgagaag ggtttgaaat caaggaatta ggcatcgaac aagaagtttc taagcgagta
     3961 gagtatcaag tggcgcagcg gatcgttcaa cgtattgagg taatcgcagg tgtatcgttt
     4021 cccgaagaag aaattgctta tttgacatta catctcatgg ctaagtcgaa tcacaaagaa
     4081 aatcaaaatg atcaggaact atcaaaggaa ttagcgaaag ttctgaaaag attgtctcag
     4141 gaatttcatt tttcattagt agaggatctc cagcttttca atggactact cgatcatttg
     4201 tcaccgatga tgattcgatt gagccgaggt attcgcattg agaacccatt gactgaggag
     4261 ataaagaaag aaaatcccaa agtctttgac gcagtaaaaa ggcatttcag caacatgccg
     4321 gctttgaaaa actataccat caatgaagat gaatgggcct atttggcttt gcatctcatg
     4381 gcggcattgg aaaaagaaag agctgcgcat aaactacatg cattgattat ctgtgcaacg
     4441 ggttacggca gtgcccaact attaaagaat cgcgtagtca gtgaattcgg taaaaatatt
     4501 acagtggtaa gtgtaaaagg ctattatgag atcaatgaaa caactttaaa aggaatcgac
     4561 ctgattattt cttctattga tctttcgaca atgtttttca aaattccagt tttacatgtc
     4621 agtatttttt tgaatgaaga tgatgttcgt aaaatacgaa aggtagttgg agaatctata
     4681 ccctatcatt cctcagatag accgatacca gcattttctc tacagcaaaa aaagcagatt
     4741 tacaccgaac agttgtctga aaaatttttc aagacttata cgtacgaacc gaaaaaggaa
     4801 gaaattcttc atgatttgct gcaaaagctg agcataaatg aagaagaagg atatataaga
     4861 gaaatgcgaa agcagatcca gcaacgtgaa aatatgggac aaatcatatt cagtgataca
     4921 gtggcagtcc cacatcccgc tgttccagtt ggaatcgaaa caaaggtagc cgttgcattg
     4981 attccttctg ggatgcagtg ggatcaatat caagaaattc attttgtttt tttagtttcg
     5041 ccttcttatg tcgaaaatga aggaattacg gtcgtgacta aagcaatcgt taggttagtt
     5101 gaccagctaa aagtacagca ggaaatctta gctgaaccta tttttgagaa tttcagcagt
     5161 aaatttatca aactgatcga ataaggagga aaacagatgg ctgaaaaaat aaacagtgaa
     5221 acactacaag tgacttcttt tgatatcatt ttccatagcg gaaatgcacg gacactgata
     5281 catgaagcat ttcgctcgat gcgtaaagag atgtttgaag aagtagaaga gaagttgcga
     5341 caagcaaatg aagagattct tcatgcgcat cagtcacaaa ctcaacttct gaaagaatat
     5401 gccagcggga caaaaataga aatggaaatt atcatggtcc atgcacaaga tcatctgatg
     5461 acgacgatga cattattgga agttgcacag gaaatgaatt atttgtatca aaaaaataaa
     5521 tagaaaggaa ggatcattgt gcaggataca gtaaaaagga aaatcgaatt agaaaaaaag
     5581 caacttagtt tgaaaaacat gtatttcaat cgttatctaa tcgtacgtta cctgactgcc
     5641 tttttctttt tcatgaacat gcaatggatg attctgcttc tatcagctaa aagtttagga
     5701 agtagtttac cgatgattct gattttagct attttgcctg cagtaggaga acaagtaaag
     5761 ctttatcgaa aacaccaaac gaacgttcca tggacaaagt ggtatttttt gttccagagt
     5821 gtctttaatt tattagtgat tccagtgttg tttacctcag gatttacatt cttgtatcct
     5881 tttatggcaa ataacaatgg aggacagtta tttgttttca ttcttgttgt aagcggtatt
     5941 tttgccagtg catttataca gtatcgttta aagaaaattt cactcaacca agaccaacag
     6001 tatatccgta ttaaacaata tgaaaaagcg ttatatttag gaaaggagaa taactaatgt
     6061 ttgatttttt gcagaaatac ctgatgggac ctatggggaa aattgcgcaa cttaaaatcg
     6121 ttcgggctgt catggcagcg gggatggctt ctattccttt cacgattgta ggctcgatgt
     6181 ttttggtttt aaatattttg ccattaccgt tcccattttt agaaggattt tttaatgcaa
     6241 cttttttcaa agttagtgac ttgtacatga tcgtcaatac aatgacgatg gggattttat
     6301 ctatttactt tgccattgtg tttgcttatg aattaacgtc aattgaacga gatgaacaaa
     6361 aattaaatgt taatccgctc acgggggcat tactatctgt ttttgcattc tttatgtgta
     6421 tccctgaatt gatcatgact gatggtaaaa tcagcttgat tgcttctatc aatgagacag
     6481 aaactgtagt aaatggtatt aggatgggct cttttgtcga acgtttagga acttcaggaa
     6541 tctttacagc tattattatg tcgattattg cggtcgaatt gtattgtatg tgtgtcaaga
     6601 gaaattgggt cataaaaatg ccagatgttg ttccgccagg agtttctcgt tctttcactg
     6661 ctctgatccc aacatttgtc attgcgtttg tggtgatgat catcaatgga ttattagttg
     6721 cactaggaac ggatatcttc aaaatgattg ctatcccatt tgggtttgtt acgcagttga
     6781 cgaatacatg gcttggtgtc atggtgattt atttcttgat ccatgcattg tggatcgtag
     6841 ggatccatgg agcgaatatc atcacttctt tcttgacccc gatcgtatta gctaatatgg
     6901 cagctaacgc acaaggcgca aattatccgt tggcaggaga attcaacaat tcttatgtaa
     6961 ccgttggtgg atcaggtgcg actttaggat tgatcatctt tattgcattc ctcgcgaaat
     7021 ctgatcagct gaaagtgtta ggaaaagcct cacttgtacc aggattattc aatatcaatg
     7081 aaccaatcat tttcgggatg ccaatcgtct acaatcccta tttagcggtt ccgtttttct
     7141 tagctccgat ggcttctgct acactcgctt attttgccat tcggctggaa atcgtcaaac
     7201 cgatgcttgc acagatgccg tggccttcac cagtcgggat cggagcattt gttggtagtg
     7261 gtggtgattg gaaagctgct gtgttagcag tcatttgtgc aatcatcgca ttcattatct
     7321 ggttcccatt tatcaaattc tatgataata aattattaaa tgaagaaaga gaaaaggcag
     7381 cagaattagc tagtgaaaag ttaaaatgat ttctaagaaa aaaggaactg tgacaaaaaa
     7441 tcacagtccc tttttttctt agaaatggga aaccactagt atataaatac cattattctt
     7501 caatcaaccg cttcacgatt gcataatccc cttcataagg ttcatctaca ccattgattt
     7561 tttgataggg atccacgcct tttcccgcaa taacgactga atcttgcgga gcgctttctg
     7621 ctaaagcgcg tcgaatcgct tcaggacgat ccatttcata atgaacgatc aaatctggat
     7681 tagtaattgc ttcattgatt tcttccgcga tttttttcgg atcttcaaat gctgggtcat
     7741 ctgccgtcaa aaaggcgatg tcagctgttt ctgatagcac ttttccaaaa tcatgtcgac
     7801 gggagattgc tttgtttcct gggctgccta atacaacaat tacacggcca tctggatgtt
     7861 catttttggc aaatttcagc aatgttttca agcttagata attatgtgca tagtccacat
     7921 agacatgcgc tccattggat tggatcaatt gatccattcg gcctggtaca cgagcttctt
     7981 ttaatccgtc ttgtgcatct tgactggagg cacctgttag tgctgcggca atgatcgcac
     8041 tggctgcatt ttcttggttg aatcctccag ccagcaaaat atcataagca gtatcgcaga
     8101 tagctaagcg atcttcatga gaagataaag taaaaccgtg agtaccttta tctgaacgga
     8161 tcacttgata attgctttct tccgaacgac cataagtgat cgttggaatc tggaatagat
     8221 cggctgtttc cctaagtaaa tcaaaatagt cactttcatg atttaaaaca actgtttttg
     8281 aattcaagat caattgtcgc ttgcaataga aatagtcatc aaatgtcgga tgttcgatcg
     8341 gactgatatg gtcaggcgag atattcaaga agattccgac atctaaagtc aagccgtata
     8401 cgcgttgtgt tttgtaagct tgtgaagaaa cttccatcac tagatgagtc ataccatttt
     8461 caactgcttc tgacatcata cgatataaat ctaatgactc aggcgttgtc aaatgggatt
     8521 tgaaatatgt tttgccatct aacgttgtat tcatagtaga aagcaatgct gtttttttat
     8581 ttgttgtgtg atccaagatt gcttttgtaa agtacgcagc agttgtttta ccttttgttc
     8641 cagtgaatcc gatcactttc aatttatttt gcggatagtc gtaaaaagcc atagcaagaa
     8701 gcgccattgc tttacggata tccgttacga tgattcctac tgctgaattt tcgtatggtt
     8761 gttctgatac ataatattga ataccctgtt cgattgctga atccaaatat tcttctttga
     8821 aattcattcc tttacagaaa aacaaagtag aagcatcagc ttttcttgaa tcataactta
     8881 actgaagaaa ttcagctttt tctggtacat caagatgcca gcctcttgtt gaaacaaatt
     8941 cttttaatag attttctttt aatagtaaat gacggatgtc ttctaaagag aagctcattg
     9001 attgatccct cgctttattc aatttcttgg tttattatag cacactcttt ttaatatatc
     9061 gtttgtttcc gctatgacaa aatccagctt tattcaaatt ttcttgacac aggggacaag
     9121 cggatgagta ttttcataaa caatgaactg actgtaatag ataggagaac gaatagtgtc
     9181 tccaacaatc caaaaacata ataattcaaa aagacgataa gactattggt aagtaaaagt
     9241 ccttgcggca ttggaagccg aaaaaatttg tggaaaataa tcggcggtat cgttataccg
     9301 ccatttgagg ccccgactgt gtacaatgtg taaaccccaa gactgaacaa aagacttcct
     9361 gaaatcagtg aaaagaaatg tgaggagatc atttccactt ttggaacaat ctctaaaaca
     9421 acaggaaaaa gcagacttcc cgctacagtc ttaaaaaata ctgattttcc taataggaca
     9481 gcggctagaa gcagcatcat aagattgatg ctccaaacag taactgagcg ttggatagaa
     9541 aaggccgttt caactaaatg tccgatggcg cccactccgg cggaagctat atcgtgaggg
     9601 agcaagaacc aattcaatcc aactgtgata aagaaaagac ctaaaaatat aacgggtagt
     9661 ttttttattt cttgtgtcat tgatatctcc tataaatgtt agccataaaa ggctgtatat
     9721 ttctttactt taccaaaaaa acgaaaccga attcaaaaaa acaattgttc ttataaaagt
     9781 tatataaaat cgattgccag tttctgtttt accaattaaa atgatagtaa tgttttgtag
     9841 aaagttggaa tctgaccgat ggataatcaa agaaaaccag cattagatca attaacaccg
     9901 caagaaaaaa tggcacgagg ctctgcttgg atgaccgcca gtaatatcat ttcaagattg
     9961 ctaggcgctg tctatatcat tccctggtat gcttggatgg gcgaaaatgc taaggctgcc
    10021 aatggattat tcaatatggg atacaatata tatgcattgt ttctactgat ctctacagca
    10081 ggaatacctg ctgcgattgc aaaacaaact gcacgctata attcattgaa tgaatatggc
    10141 acttcacgac gattatttat ccgtgcatta cagatgatgg gcgggttagg cctgttgttt
    10201 gctttgttca tgtacatcgc ctctccttgg ctagctcatg cttctggtgg cggagaagaa
    10261 ctgattccaa cgatgcgttc tttgagtatc gctgtattgg tttttccttg tatgagtgtg
    10321 atacgagggt atttgcaagg caatcaagaa atgatgcctt atgcgctgtc gcagattgta
    10381 gaacaggtgg cacgtatttt ttatatgcta ttggctacat tcatcatcat gaaagtcatg
    10441 gatggtgact atgtgacggc ggtcacgcaa tcaactttcg cggcatttat cggtatgcta
    10501 gcaagtattc ttgtcctttt gtattttttg aagaaacatc aagcgtatac atcggcgttt
    10561 attcactaca gcgaaaacaa aataaatatt gcaacgaagg aattgttgtt agataccgtt
    10621 aaagaagcga ttccgtttat tattgtgggt tcaggggtaa cgatcttcaa attagtcgat
    10681 cagttcactt ttatgaagat catgtctgca tccaccgagt attccaatgc acagttactg
    10741 gatctttttt cgatttttag tgccaatcca gataagttga cgatggtagt tattgcattg
    10801 gcaacatcga tcgcgtctac tgggttaccg ttgattacag aagctgttac gttgaaagat
    10861 cgagtaggtc tggcaaaatt gacaagcgga aatttacagt tgttttcatt ttttatgttt
    10921 ccggcgacat ttggtgttat gttacttgcc tatccgctaa atacattatt ttatacaccg
    10981 gattctctag gaagtaatgt actgatccaa gcaagttttg taggactctt cctaggacta
    11041 tatatgctag tttccaacat gctgcaagga atgtttgaaa acaaggcggc aattaattat
    11101 ttggttattg ggtttctagt gaaactcctt cttcaatacc cagccattcg tttatttgaa
    11161 gtatacggcc ctttattggc gacaatgatc ggatttgctg tgtcttgtac cttgatctta
    11221 aagagaatcc atgaagtagc ccgctttaaa ccaagttttg tctggcgcag gacattgctg
    11281 atctttattc ttaccttgat tatgcttttg gcttcgtgga tcacaaagat gatttttggt
    11341 gcttttttaa acgaagatag caaattccag tctctattgt tgattcttgt agtagcaggt
    11401 ataggggctt tagtgtatgg ttatttagct ttgaaaatcc gcttggcaga aagactacta
    11461 ggaccaggaa tagcgcgttt acgacataaa ttaaaaataa aataagtgaa tcagaagagc
    11521 tagattgatg ctcttttgaa atgggggaaa cgcatgcgtt tagataagtt tttagcagat
    11581 atgggacttg gcagccgcaa agaagtcaaa ggattaatca aaaaaggaac gatcgaagtc
    11641 aatggacaag tggtgaaaag cgataaagcg caagtaaatg aattcgaaga tgcggttact
    11701 tatttaggag aaatcattac gtatcaaaaa gatttttact acattttgca taaacctgca
    11761 ggagtcattt ctgctactca ggataatcat gatcaaaccg taatcgattt attggaagac
    11821 caggattatc gggaagatct ttttccagtc ggccgattag ataaagacac agagggcctc
    11881 ttgattttaa cgaatgacgg ggtgtttgcc catcagttac tatcacctaa aaaacatgtg
    11941 gagaaagaat atctagctga ggttagcggt ataatgacag aagaagatat ccgtttattt
    12001 gcagaaggac tgattattga tggcgatgag cagacattgc cggcaaaact ccaaattgat
    12061 caagtagatc aagagacaga aacttcaatt atccgtctga ttttgcatga aggaaagttt
    12121 caccaagtta agcgaatggt caaagcagta ggaaaagaag tcttatattt aaaaagaatc
    12181 cgtatgggaa aattttggct gcctaaagat ttgaataaag ggcaatatcg acccatgacc
    12241 aaagaagagc tagaacaagt gagagaaaaa taattgaatg gatagctaaa agaataagca
    12301 gagtagttgt agcgccatct gaaaagcaga cttgtccgtc tgactttcgg atggcttttt
    12361 tgtgaaattt cggcttattt ccgtagaagc tacttctgga acaccgtttg ttcggaataa
    12421 gggactgtga caagactttt gttacagtcc ccattgttcc ttgtgactca gagttagata
    12481 gtaaggacac gatctcccta atactcaggc caaaacgctg aattcacaac ctctgataaa
    12541 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    12601 ttttcccgtt tttttctcta atactcaggc caaaacgctg atttcacaac ctctcctata
    12661 ctttgtttca ttttccctat actttgttta tttattctca ttattgaaag cgctataatt
    12721 acgttgcgaa tgaatcgaaa ggggttttta gatgaaaaaa cgaaaggggt tcaatcattt
    12781 ttttatgaat ctatggcgtt ataaagcact tgtcctgatg gctgtaccgg gtatgctttg
    12841 gatgattttt ttcttttaca tacctgtaat ggcgaatgtc gtagcattca aaaattttca
    12901 tatttcagca gatgggttct tggctagctt gagggaaagt ccttgggtgg gttgggaaaa
    12961 tttcaaattt ttattttctt cggatcaagc atttctcatc actaaaaata caattttata
    13021 taatgtagca tttatccttt tgaatctgct gatctcagtc gtatttgcaa tcattatgag
    13081 cgagttgaga aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt
    13141 tctatcttgg gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat
    13201 attcaatcag tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa
    13261 atattggccg tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat
    13321 tatttacttt gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga
    13381 tggagctagc aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat
    13441 gacgatttta acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta
    13501 taacattcct aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat
    13561 ctacaatggc ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca
    13621 atcagttgtc ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag
    13681 cgattctgca ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa
    13741 gtccgctctt tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct
    13801 atttcctgta ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata
    13861 ttgtcgactc acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac
    13921 ttgttcgacc agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact
    13981 gttttaggta cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg
    14041 tcaaactttc cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca
    14101 ccagggatgg tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt
    14161 tgggcattga tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc
    14221 tttaaacgtt cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa
    14281 ctgcgaatat ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt
    14341 ttatttgccg cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat
    14401 gataagctag ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg
    14461 acacaaaatt caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa
    14521 tctgccagaa tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt
    14581 ttccagaagc attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa
    14641 aatataaatc ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac
    14701 aggtgtttca gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga
    14761 ccaggcagaa gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga
    14821 taatttcgat caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa
    14881 agtcaatatc aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc
    14941 ttccggggaa aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg
    15001 agcctttgct gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt
    15061 agatgaagca tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa
    15121 tggaaatgtc tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga
    15181 tctttcaatt gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca
    15241 tgaaaaagaa ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa
    15301 ctttgattat cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc
    15361 gaaaatcatc aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag
    15421 ctggtacaaa caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact
    15481 agaaggaaat acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac
    15541 aattttgaca aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa
    15601 atctacagct caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga
    15661 aaaatcagta gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt
    15721 ttgggggatc gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt
    15781 gctagatggt tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat
    15841 tctatataca caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc
    15901 tgatgcagaa acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga
    15961 attaagtaat atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt
    16021 tgatccagat gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa
    16081 ggtattgaaa gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa
    16141 gacaaagggg ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca
    16201 aaagccagtt tctcggtatt aagctaaata tatgtaaaaa tgtggaaagc catattcaca
    16261 aaatttttct tactactcga aactaagcgg ctatttcaca aactcttggt aaacggtgtt
    16321 caatcagcta accctcggta ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc
    16381 gtttgtttct ctaataatca ggtcaaggtg ctgattgccc aacctttttt tgtataatga
    16441 gaaagtataa acttaatgtt tggaggagaa gatatgttag ggaaggtttt ggaagagtta
    16501 tttatccgga tctgggtcat tataaaacta accttgtatt tctggatata tacatttgcc
    16561 ggcgggatca tctttggatt aggtgcggca tggaaaacag tcaatgaact attttatttg
    16621 tacggattcg agtataaaga aatcacaata aaaagaggct ggaatatata taaaagaaat
    16681 tttttaagag gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac
    16741 aatttatatt tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta
    16801 ctttttgcct tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa
    16861 tatacgatct ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt
    16921 tcgtcttttc taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc
    16981 aaaggattga tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca
    17041 acacattggc gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct
    17101 attttcaaaa ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg
    17161 tgtaacgatg actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca
    17221 ggcagaaaac atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac
    17281 aaccttgagt aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt
    17341 gacgacctac ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa
    17401 cagtaccgat gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga
    17461 agaactaagt gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag
    17521 agaaaacaaa ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat
    17581 caagttgcct atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa
    17641 gaaagaacta gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat
    17701 gatttctgga acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac
    17761 gtttgaaaga caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat
    17821 tgacgatctg gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat
    17881 attagctgta ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct
    17941 gatcattggt atcattattt tagacaatat actgcttatc tttctttata aaacattcaa
    18001 acgatattca cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa
    18061 tttagagacg cggatcgaca taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg
    18121 tatcaacgaa atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat
    18181 caaacagcaa gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata
    18241 taatacatta gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga
    18301 tgttgtgtat gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac
    18361 tttaaaagaa gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc
    18421 taatcgagta gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa
    18481 atttgtgatc cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt
    18541 cgataatgcg ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa
    18601 agataatgga aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca
    18661 tcctaaagtg gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc
    18721 cagcttcggc agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt
    18781 ttctattaca ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac
    18841 atgattttga atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta
    18901 gcaacagcag agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta
    18961 gtagtaacgg atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa
    19021 aaagaacgtc ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg
    19081 aaaggaggta tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa
    19141 ttggttgaaa gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat
    19201 caacaagaaa tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa
    19261 ataagtgaag aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt
    19321 caactggcaa gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc
    19381 ttttactatc aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat
    19441 gaagttgatg gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc
    19501 agtatcgggg aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa
    19561 gacaatctac agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta
    19621 caagcagaaa caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat
    19681 aaacaattgg aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca
    19741 gccatgatgc cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg
    19801 gaattgattg atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca
    19861 aaaacggtgc aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag
    19921 aaaaagtaca gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa
    19981 ccacttacac tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag
    20041 ctgttcaaaa aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa
    20101 aaagcgaaac aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt
    20161 ggctacaaca acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct
    20221 aaagagtacc gagaaaacag tggagaatca taaaagaaaa agccaatgta atgaaataac
    20281 ttataaaaaa ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag
    20341 ctttttcatg acctctcgat aaacggagtt caaaagccag tttctcggta ttaagccaaa
    20401 tttatgcaaa aatgtggaaa gccattttca caaaattttt cttaatactc gaaactaaac
    20461 agctttttca cgacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc
    20521 aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga
    20581 acgctgattt cacaacctct caataaacgg tgttcaaaag cttgcatctg cggtaataag
    20641 accaaccaaa cgaaaaatat gaggatctat ttttgcttgg ctgttcttat tacttgatgc
    20701 aggacagctt tttcacaacc tcttgttaat tatagtttga taaggaaaaa ggcaaatctt
    20761 ttgcttgata tttcttaggg tgagggatga tccctaattg gtaagagatc gtgccgcctt
    20821 tcagcaaatc ttgatgggta aaatataatt tcgtatattc tttgtccatc cgtttgattt
    20881 tatggataaa ttgttgctgc ggccgattag gcgttgcttc aatcgtcaat tcctttccat
    20941 ttgacaaaag caaaacagcc ttgtcaatta atggcatacc gataacatat tcaccggttc
    21001 caggcgtgac aggataaaat cccagactat tgaagatata ccaagcggcc atacttccat
    21061 tgtcctcatc tccagggtaa cccattggag aatcgtcgaa ggtctgagtc atcaactgtt
    21121 taagcagtgg ctgtgccatt tcaggttgtc ctatataact gaacaagtaa ggatagtgaa
    21181 aacttggctg gtttgaaata gctaattgtc caaattctat cgcggccatc tcactcattt
    21241 cgtggatctc aaaaccatat cctcctgggt caaacgttgg gggctgattg cacagctcaa
    21301 tgagtttttc tttatatctc tcactgccac catattttcc gatcagtcct gaaaaatcgt
    21361 gataaacagc aaagctgctt tgccaagcac ttccttctgc ataatcttgt ccccaacgat
    21421 tagctgaaaa tggttctctg aacttgccat ctttatcttt tgaacgcata aatcctgact
    21481 catagtcaaa gatattttga tagttcaatg attgctgctg atatttcttc gtcatttcct
    21541 cttcttctag taccttggca acttggctaa tacaaaaatc gctataagca tagtctaatg
    21601 tgtgattgac tgattcgtgg tagtgattag gaacatagcc atatgtcaag taatcttctg
    21661 ttccttggcg tccataattt ggatgtttgc tttgaaccgt agcacctttt tccattgccg
    21721 ttagaaattc aggcatcaag tctcgtcgga tatcttttac agcagcatct gcaatgaccg
    21781 catcgatcaa tgtcccaggc ataagtccac gttcatcagg agacagccat tttggcaaat
    21841 agccactttc tctatagctg ttcaaaaaac cttccagcat ttcttcgtat ttttctacag
    21901 caatcaaaga ataaagagga taaaccgttt tatacgtatc ccaaaaccca ttatttgtat
    21961 agagaacacc aggttttata gatcgactag tcgtatcata gtggattttt tgcccactag
    22021 gatcaatttc ataaaatgtt tgtggaaata aaaacacccg atataaatta tgataaaacg
    22081 tccgcaattg ttgttgatca tggtgtgtga gctgtatacg atcaaaatag gactgccatt
    22141 gttcttgact gtgagcaagg taagtctctt tgctttggtt ttgttctagt tccaggttat
    22201 aagctgcttg ttccgagctg ataaaagatg tcccaaatcg gatagtttgt ttttttgtct
    22261 gttcaaaata caacgggatc gagtcgctat ttttgttgga aacagctaac tcatcaattg
    22321 ttaaaggata ttcaaatctc aatataaaat aaaaagaaaa atcaggatct tctgaaccag
    22381 caaaatgtgt gatttttcct tccactgtgt aaggatctat aacaaagagc tgatggcgtc
    22441 ccggaaagga taaaagtaaa ccgctgtcat ttttgctgta atcaatagat aagattccgc
    22501 catacatgct aggtatcaat gtagaacaga tgccataacg taattgacag atcgacagat
    22561 gagtcgggca aaatatactt tcttctgggc ggtaagaact ttgagcatgg aaaagggtgt
    22621 tctcctgtag ttttccattg atcggcgtaa acagcatatg actgaaatca cccatccaag
    22681 gacttggttg atgggtaaga cggtagcctt gaaaaacgcg gtcatcagga tgaaaccacc
    22741 agctgccgtt ttgatccgta gtttgcggtg caaaataatt catcccaaaa ggaacgccag
    22801 tataaggctg gcagttccca ttagagaaat ttggctgatt cgctgttgca tgtctagtat
    22861 ctatttgacc gatattcatt ttatttctcc tttgttgatt gtcttttgat gaatgaaact
    22921 ggaaatttac tatcggatgg tcgatctttt gtctctaacc aagtgaccaa agcatctccc
    22981 gcaagcttac ctaactgttc aaaatcttgt gcgatggttg tcagtggcgg gtcaacaaga
    23041 gctgctgctt ggatatcatc aaatccgata attgaaaaat cttcagggat atgataacca
    23101 tgctgtctca agagattcat ggtctggata gcgacaacat cgttttcaca aacaaaagca
    23161 gttacttcat acttagtgat ataagaaagc aattcatcta atgtcgcgct tttgtcatcc
    23221 agtgaagtgt ggaaagtcaa accagctttg tccaatgctt caaggtaacc aaggtaacgc
    23281 tgtcgaacgg attgtggatg agtctgcgta ccaaacaaat aagcgatttt tgaatgtccc
    23341 tgttcgatca aataagaggc agcaagcgca ccgccttgga aattatcgga aagaacggta
    23401 ggaaaaggta actcatagat ttttttatct aatatgacga cagggaattc tttcaatgaa
    23461 agctcaaaaa gtaagtcaag atgttgttct gtatcaagag cataataaat caatccgtca
    23521 aattcattga ttagctcttt tgctgtttta ttttgtagaa agtcaagcgt cgtcatcatg
    23581 acatcaatct gtttatcttg catcacaggg ttcaatccct tgctaaaatc accaacagac
    23641 aaatcagcga catatggaag aagaaacaga atccgtcgtg tttttgtagg agaagcaaga
    23701 atctgttccg gagaagcgga tttaacgaaa ctgcctttcc cgcgaacgcg ataaataagt
    23761 cctaattgct ctaattcagt caatgcacgt ttggaagtga ttctgcttac tttataggta
    23821 cgtgaaagtt ctttttcagt tggtagttgg ctgtctacag gtaattcacc agttaaaata
    23881 gccgtttgca aatcattaaa tattgtctgg tacaatg
//
LOCUS       DACDHJ010000024        23586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDHJ010000024 DACDHJ010000000
VERSION     DACDHJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.024"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(462..662)
                     /locus_tag="IV223_000745"
     CDS             complement(462..662)
                     /locus_tag="IV223_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8711052.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(967..2199)
                     /locus_tag="IV223_000746"
     CDS             complement(967..2199)
                     /locus_tag="IV223_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8711053.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2453..3022
                     /locus_tag="IV223_000747"
     CDS             2453..3022
                     /locus_tag="IV223_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8711054.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3200..3640)
                     /locus_tag="IV223_000748"
     CDS             complement(3200..3640)
                     /locus_tag="IV223_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8711055.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3798..4562
                     /gene="map"
                     /locus_tag="IV223_000749"
     CDS             3798..4562
                     /gene="map"
                     /locus_tag="IV223_000749"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8711056.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4594..5517
                     /locus_tag="IV223_000750"
     CDS             4594..5517
                     /locus_tag="IV223_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8711057.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5593..6732
                     /locus_tag="IV223_000751"
     CDS             5593..6732
                     /locus_tag="IV223_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8711058.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6725..7525
                     /locus_tag="IV223_000752"
     CDS             6725..7525
                     /locus_tag="IV223_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8711059.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7525..8352
                     /locus_tag="IV223_000753"
     CDS             7525..8352
                     /locus_tag="IV223_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8711060.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8330..9064
                     /locus_tag="IV223_000754"
     CDS             8330..9064
                     /locus_tag="IV223_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8711061.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9164..10030
                     /gene="rfbA"
                     /locus_tag="IV223_000755"
     CDS             9164..10030
                     /gene="rfbA"
                     /locus_tag="IV223_000755"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8711062.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10044..10616
                     /gene="rfbC"
                     /locus_tag="IV223_000756"
     CDS             10044..10616
                     /gene="rfbC"
                     /locus_tag="IV223_000756"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8711063.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10638..11666
                     /gene="rfbB"
                     /locus_tag="IV223_000757"
     CDS             10638..11666
                     /gene="rfbB"
                     /locus_tag="IV223_000757"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8711064.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11764..12615
                     /gene="rfbD"
                     /locus_tag="IV223_000758"
     CDS             11764..12615
                     /gene="rfbD"
                     /locus_tag="IV223_000758"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8711065.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12650..14683
                     /locus_tag="IV223_000759"
     CDS             12650..14683
                     /locus_tag="IV223_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711066.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14727..16007
                     /locus_tag="IV223_000760"
     CDS             14727..16007
                     /locus_tag="IV223_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8711067.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16217..17023
                     /locus_tag="IV223_000761"
     CDS             16217..17023
                     /locus_tag="IV223_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711068.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17035..18255
                     /locus_tag="IV223_000762"
     CDS             17035..18255
                     /locus_tag="IV223_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711069.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18245..19831
                     /locus_tag="IV223_000763"
     CDS             18245..19831
                     /locus_tag="IV223_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8711070.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19870..22008
                     /locus_tag="IV223_000764"
     CDS             19870..22008
                     /locus_tag="IV223_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8711071.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22376..23359)
                     /locus_tag="IV223_000765"
     CDS             complement(22376..23359)
                     /locus_tag="IV223_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8711072.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7440 a         4031 c         4826 g         7289 t
ORIGIN      
        1 gatatcaaac aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa
       61 atagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa
      121 aacttctgaa ttccatactt tataaaaatc tttgataaat ctattcgaag ggtcaaattt
      181 aaaccataat tgtgtttttc cttccatcag cataaaagtt cacctcaaaa agagtatacg
      241 aacgaatgtt ctttttgtga aggcggaatt taatatactg aataatagaa aacaagtact
      301 tgtgccaaat tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa atagaaaacc
      361 ttctatatta agatttcctg caataacaaa cacaacagaa gacgtgtaat agttagtcag
      421 gaacgtacaa ataacccctg tatcctttgc ggtacagggg gttattttgt ataagtagac
      481 attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct
      541 tgataaagta aaatagatgc atgcgtatgt cttagtccat caataaacag tcgaagagaa
      601 aatagggaaa aattgattac taaagaaaaa cttactaggt cgacggtgta gttgattttc
      661 atacttcaat agattcgaat taaacgattt cgattggctt ataccagtcc tatccaacga
      721 aagatttatc gttcttaact ctagcggtat aacttgaagc tttacgtaaa gccaatggga
      781 atgaactgga gatgactttt tattattttt agtaatagct gatgggttaa tacttacaac
      841 tttccttttc aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa taaaggatct
      901 aagccaatag cttagatcct ttattctatg ctttatatta ttttttgcat gtgtgcgaaa
      961 gtatactcaa gcccaatcgc catttcggaa aatcggtact gtagagccat cttcacggat
     1021 tccgtcgata tccatcttgt cagatcctac catgaaatct acgtgggttt gacttctatt
     1081 caatccagct tctgctaatt cttcatcgga catttctgta ccgcctttca cactgaaggc
     1141 ataagcagaa cctagtgcta agtgattcga tgcgttttca tcaaataatg tattgaaaaa
     1201 gatgatgcca gattgagaaa taggtgaagg gtcaggtaca agtgcgactt ctcctaaacg
     1261 acgcgcacct tcatctgtat caagcagttt tgctaagaca tcttctcctt gttcggcaga
     1321 aaaatcaaca acttttccat ctttaaaagt aaatttcata cccgagatga tggtgccagc
     1381 ataactcagt ggctttgtac ttgaaatgta accatctact cgatgactgt caggtgctgt
     1441 gaacacttct tccgtcggca tatttgccat gaatttttct ccacgagcat tatagcttcc
     1501 agcgccttcc caaagatggt ttttaggcaa tccgataatg atatctgtac cgggagcagt
     1561 atagtgaagg gcagaaaatt gttctcgatt caattcttct gctttttttg ctaatttttc
     1621 atcatgcttc ttccatgcta agacagggtc ttcttcatag acacgagtcg ttttgaaaat
     1681 ctgatcccat aatgcctcaa cttgtttttc ttctggcagt tctgggaaga cttttgctgc
     1741 ccattgttta ccagccgcag ccacgacagt ccaactgact ttatttgctt gagttgcctt
     1801 gcgtaaattc attagtgctt tgccattcgc tgattgataa gacgctactc gatggctatc
     1861 tacaccagca aatgcatcgg gatcggcaga aacaacgctg atcctgcttg ctcctttttc
     1921 aagccattca tccgcttgat cgattttaga ttgtggtaca ttctcaatac gatccgttgc
     1981 agcatgtaaa agaaattctc tttggatctg gtcgtcagtc cattgaacga taacctcagc
     2041 ggctcctagt ttataagctt cttgtgtgat caatcgagca aggggtgcct gttccacgct
     2101 gatctgtaaa acaactgtgt gtcctttttc tgtggctacg ccagtttctg caattaagcg
     2161 tgcatatttt tttagaagtt cattaaaatc agataacata ataaaaattc ctcctcacca
     2221 ttttttctgg taactattag catgatacca tttaattatg attcaaacaa tgaggcttta
     2281 aataaaaaaa ggaggttatt tttttaaggt taagtttcat gatatatgtt actacttgct
     2341 atttcagctt gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac atgttacaat
     2401 gaaattaatc aatggtggaa gcgttataaa aaaattaagg agattaaaaa atatggcaag
     2461 aaaaaagacg attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga
     2521 tggatttaca cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc
     2581 gatttatctt gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta
     2641 tgatcattta caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct
     2701 tgcttttaat tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtgctt
     2761 agaagaatat ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac
     2821 agtgaaacgc gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa
     2881 tgggacatgg atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc
     2941 aactgatgaa cagattgaaa agatgatcca agacagtata gatgctatct tgaaattcaa
     3001 agaaccaatc gaaatcgatt gaatatactc aaaagttcca caaatcagta ggtaacaaga
     3061 gatgtgtcaa aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga
     3121 agaactcttc catgaatgca atgaataaaa gagccataca gattgaataa atctggatgg
     3181 ctcttttatt gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat
     3241 tttcgatatc ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg
     3301 cgcctgtttt tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa
     3361 aagtatcacc agatccgcag acaccgtaaa tttttcccaa tagatccaat tcttgcaagt
     3421 cttcataaaa gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta
     3481 ctacgcaaat atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt
     3541 cgacttcgat atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt
     3601 ttcctgtcat gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata
     3661 attatttaac aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga
     3721 cacctgggga atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt
     3781 aaaaaggaga cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag
     3841 aatctggagc tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa
     3901 tcacaagttg ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg
     3961 ctgcgcaaat tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa
     4021 tttgtcatgg attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata
     4081 tgtgtatcga tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat
     4141 ctacacctga gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg
     4201 agcaagcaca agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag
     4261 aaggcgaaaa tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc
     4321 atgaagaacc agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag
     4381 gcatggttat cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc
     4441 ctaacggctg gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat
     4501 tggctattac taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt
     4561 gatctatcca gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa
     4621 aatgaaaatc tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg
     4681 agtacgacct ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt
     4741 gcttttggta atattttacc gtttctccac atcgatcaag agactgtact gacctattta
     4801 cgagaagtga ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg
     4861 acacaaagtt caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt
     4921 caaagtatca atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa
     4981 aattttttga tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt
     5041 agtggggtaa ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc
     5101 gtacaaatac ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg
     5161 gtagctttat ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag
     5221 ttgaagacga tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa
     5281 gtgtttagta tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga
     5341 agttttattg tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga
     5401 atcatcaatg cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat
     5461 ccaagccgaa aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa
     5521 caagagttct tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa
     5581 gtaggtgccc atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta
     5641 ttatcactga tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac
     5701 aaaccaaatg aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc
     5761 tttatttcat ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat
     5821 attgtaccga tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa
     5881 tatgagctga cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat
     5941 tttgtagcag atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta
     6001 ggctggctta gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat
     6061 ctgattgacg gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata
     6121 ggaatcattg gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc
     6181 tgtcttgtag caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat
     6241 ttaggcgata caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg
     6301 ctgaagaacg tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta
     6361 acagacacgt tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca
     6421 gataaaaaac atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc
     6481 ctgacgattt atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact
     6541 ggaagaatcg gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt
     6601 gaattgattg gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta
     6661 gggaataaag agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat
     6721 atagatgaat aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga
     6781 taaaattttt cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa
     6841 aatccgaatt tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag
     6901 gaaatatccc tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca
     6961 taatcacaat ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt
     7021 agtggatgaa gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc
     7081 gatgttagca cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca
     7141 gctggctgtt tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt
     7201 tgacaaacga ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt
     7261 acggatgatc tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg
     7321 gtttgatgag cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa
     7381 agcaggttac aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg
     7441 tgcgcatcga aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa
     7501 taagtggggg tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat
     7561 cagcaactat tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag
     7621 caaattcagc ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa
     7681 aaagaaaata tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat
     7741 tttgcgttgt ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg
     7801 aggctagtcg acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt
     7861 gctgcagttc cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt
     7921 tatatcaatc gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg
     7981 gcaatcaatt caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat
     8041 cctgcttttt ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa
     8101 aaaactgtgg agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa
     8161 aaagtttcaa gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa
     8221 tatgatcaaa ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag
     8281 ttcttgaccg tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat
     8341 tggaaggtgt gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt
     8401 aagagagcag ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc
     8461 tgatgatggg tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa
     8521 gcgaatccag cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat
     8581 ccagcagacg aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac
     8641 aaaagtgaaa aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc
     8701 tgatttaatc attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg
     8761 aaattccaaa gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat
     8821 ggctttccgg tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca
     8881 tgatatgtgg attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac
     8941 tctgtaccga agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca
     9001 aaaaatttct tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg
     9061 ttgataaaag atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata
     9121 ataacaaagt gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct
     9181 tgctgggggc agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat
     9241 gccgatttat gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat
     9301 caaagaaatt ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg
     9361 aaacggaaat gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt
     9421 ggcacaagcg tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt
     9481 aggggacaat atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa
     9541 agatacaggc gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt
     9601 agaatttgat gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc
     9661 aaattacgct gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg
     9721 tatcaaacct tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga
     9781 taaaggaaaa ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac
     9841 acatgaatca ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt
     9901 aaaagtggct tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt
     9961 gctggccctt gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc
    10021 cgctgaataa ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa
    10081 tcgtagaaat ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag
    10141 caaaatttgc agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag
    10201 ctgaagctgg tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat
    10261 tgattcgggt agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc
    10321 cgacttatgg tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg
    10381 tacctaaagg atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata
    10441 agtgtgataa ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt
    10501 tagcgattga ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc
    10561 atccgacatt aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat
    10621 taaggagaaa ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct
    10681 ctaatttcgt tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata
    10741 aactgactta tgcaggaaac aaagagaact tggccggact tcctagcgat cgcgtagaat
    10801 tggtcgtagg agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg
    10861 cagttgttca ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat
    10921 ttgtacaaac gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg
    10981 tacgctatca tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc
    11041 tgccaggaca tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt
    11101 caagtccata ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt
    11161 cttttggctt acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata
    11221 ttgaaaaatt tattcctcgc caagtaacga gtatcttaag cggtattcgt ccaaaattat
    11281 atggcgaagg aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt
    11341 ggctgatttt gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag
    11401 aagataataa gaccgtgatg gaattgatct tagaaatgat gggccaacca aaagacgcat
    11461 acgaccatgt aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat
    11521 tgcgagaaga gctaggttgg cagcctgagt ttacgaactt ccgtgatggg ttggctgaca
    11581 cgatcaaatg gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag
    11641 cagcatatgc caaaaatgga caataataca gaggaaattt cactgtatta atggaaaaac
    11701 acatgtgagg caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataac
    11761 catatgtttt tattaactgg aggaaatgga caattaggta cagaattgcg ccatttatta
    11821 gatgaaaaaa atctgccata tgtttcaaca gatgcaaaag acttggatat cacagatgct
    11881 gagaaaacaa tggcttatat tacagaatta aaacccgatg ttattttcca ttgcgcagct
    11941 tacacagcag tagataaagc agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc
    12001 gatggcacaa gaaatgtagc acttgcagca aaagctgtga atgcacaatt cgtctatatc
    12061 agtacagact atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa
    12121 acgaatccac taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag
    12181 attttagaag actattatat catacgaact tcttgggttt tcgggatcta tggacacaat
    12241 ttcgtcttca cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac
    12301 cagtttgggc gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa
    12361 gaaaaagcac catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgag
    12421 ttcgcaaaag aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa
    12481 tttccgcaaa aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg
    12541 ttaggttttg tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa
    12601 aaaatgaata agtaatttcc tttttatcaa atagaatttg gagcatgata tggaaaaaca
    12661 aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt cgaatttggt
    12721 gcttgacctg tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt
    12781 tacttttaat ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga
    12841 cgatgggaaa aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg
    12901 gctggtgcag acaccttttt acctctcaca agctgaagag gacttttcgt tggtgaacga
    12961 ggattattca ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat
    13021 tacggttatc ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact
    13081 ttccttttat tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat
    13141 gatattaacg aagaggaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc
    13201 tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg
    13261 actgatgatt gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt
    13321 gatgatgctt ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca
    13381 tcaagtgatg ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag
    13441 caagaaagtt tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat
    13501 tggcggagtc atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa
    13561 taccttatat ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt
    13621 ttctttcctt acaaattgga aaattccttt tgaagatatc aactattcga ataactctga
    13681 agtggccctg ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt
    13741 tggcaaaaaa gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt
    13801 ttctatcttt tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc
    13861 ttatgttcct ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat
    13921 ctggtttatc gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt
    13981 gttggcagta aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta
    14041 tttttcggat tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc
    14101 attatttggc tggagacgct tgttttacct cgcttttgtc gccttagtta tcgtatctgg
    14161 ctttttcgtc aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa
    14221 agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt
    14281 atataacttc accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga
    14341 tatagctgca tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta
    14401 tgctcataca agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga
    14461 tatgttttcg gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat
    14521 cagtaaacga ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata
    14581 tggaccggat aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt
    14641 accacaaaca caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata
    14701 atcctgaaat attagtagaa aggagagtga agataattca agcaatcgta caaaaatttg
    14761 tcaacaaaat gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga
    14821 cagcaggggt aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg
    14881 cttttagtgt ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc
    14941 tttatctagg tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa
    15001 atgagtcttt atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta
    15061 aatggatcga gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc
    15121 tcggaatcac gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct
    15181 gggctccagg caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt
    15241 tgatgactct tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgttc
    15301 gaatcatcgg tgggatagca gtcgttgcag cctttttagg tatttgggtc tgccaatcta
    15361 gaggtgcatt agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga
    15421 tgcgtgtcat tcgtgcgcca tttattgcct acacggttac gattctttta gctttgccga
    15481 tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt taccgatcga gaagatattt
    15541 ggcataaatt ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa
    15601 catttatctt ccagcgagga aaccaatttt taggaaacca caatagttat aattctattt
    15661 tgaatatcta tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg
    15721 gtcgcttaac gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt
    15781 ttgcggtgat gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga
    15841 ttatttatat cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg
    15901 aattctatgt tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg
    15961 aacaaccaga tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata
    16021 cgagagaggc tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct
    16081 tctgtaaaat agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag
    16141 aacgcagctc ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc
    16201 gcttggagga acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa
    16261 cagaaaatta ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt
    16321 tttaggaatc ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt
    16381 atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt
    16441 agttaccggg atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt
    16501 ttttcgggaa tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac
    16561 gacgaaaatc ctatccaatc tgtacaccca tttattcttc attctgattg gcatagctgt
    16621 cgcgcttata aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt
    16681 ttgcatgtgt gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt
    16741 acctgatatc atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat
    16801 attatggagc cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct
    16861 gtactatgtt gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca
    16921 tggaacatat actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc
    16981 agtgttccgt agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca
    17041 gaatatgcaa ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg
    17101 gatcgatttc gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg
    17161 ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac
    17221 ggatcgggaa aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc
    17281 acgatggaaa tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct
    17341 gagtattctg gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa
    17401 gaagtcgatg aaatggtcga tgatatcatt gaatttgctg acatcggaga ccatatttat
    17461 caacctgtaa aaacttattc aagcgggatg ttcgttcgtc tagcttttgc tgtagcaatc
    17521 aatgtgaatc ccgatatttt gattgtggat gaagcattgg cagtaggcga tcttgaattt
    17581 caactaaaat gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc
    17641 gtttctcatg atatcagttc gattcgtcgt ttttgtgatc gttcctattg gttgaaaaac
    17701 ggagaagtcg tcgaatatgg cgatacgatg gaagtaacga cgaattatga aaacttttta
    17761 aagaaaaaat cagttaaaac agtggatcgg aataaaaacc aagtagaacg gttcgccgct
    17821 ccagatatcg tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa tccggtggat
    17881 atgctggaac aaggagaaaa actatacgtt aaagtaactt atgaagtaaa agatgataca
    17941 atcaaaaatc cggttttagg aattgcgcta cgaacagtcg acaattttta tgtatgcgga
    18001 cttaatactc tactagatgg tatcaagatt ccttggaaaa aaggagagaa tatcttttat
    18061 ctggagtatg aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt
    18121 gaagaaaatg caacggttcc tttggtatat aagacaaagt acatgaactt gtttgtcagc
    18181 ggttcttata tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat
    18241 agcaatgaaa tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc
    18301 cgcacagatc aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gccgattaac
    18361 gaaacattta atcggagcaa aacaatgtga agtgagcatt gttgagttag ataaggaatt
    18421 gttcgatttt gtcagcgaat ttgctcaaga tggattttat ggtgatatcg aaagttttga
    18481 gtgggcaaac tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga
    18541 acatttggta gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg
    18601 agagatcctg attacattcc ctaacttggc gcataattct gtcatgatcg atttgttcaa
    18661 taaccaattg ccgtgggctt cttatggtct cctagatgaa acccataatt ctttttatac
    18721 tcatgatggc ttccaaaaag tttttgaaaa agcgggatta ttcattaata ttgaagatta
    18781 tttgtactta gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc
    18841 tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt
    18901 gatgaaacat ccagtagcac aaagttccat tgcagagcca caaaattcta attatgtaaa
    18961 agtgttagaa gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa
    19021 taatttcaca ggggaaaatg agacactaag tttccccgta tcagaaacaa cagaaagaat
    19081 ggtgtttcgc ttcgcgcaac aacctagctt tatcgaattc agcgcagaag ctgccggaga
    19141 aaaactaaca tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt gttatttgtt
    19201 tgatgggaaa gaattaccag agttcgttct aacggatatt tctgggaaag aagtgactat
    19261 ccattgtcac tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgttggaaac
    19321 gattcgtccc atggctgaag tcacaaagca gctttctgag aaaaatgacg aattagaaag
    19381 agaaaatcat catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac
    19441 aaatcgctat tgcacattgt tggaaagtga tgaatggaca ataaaacata gactaggacg
    19501 acgtaagaaa gaaacttcga aaaaaatcca agaaaaagaa ctttctatat gtattgacga
    19561 gaaaatctgg gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc
    19621 ggatcgccag cccttgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat
    19681 tcctgtttca cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg
    19741 atttgagtta cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca
    19801 aaatggccaa tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt
    19861 aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg
    19921 gccactggcg atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct
    19981 acgataaagg tagaaaaaga aaatgtgatt gctgaatcaa cacatgtcgt aagattggat
    20041 atcaatcatt tatataatct tgatgttaag acccaaagcg gtttcaaggt acgtttatca
    20101 gggaaaatga gaggaaaagc catcttagac ttccaaacag cgagacatca aaatggcata
    20161 gctgtgaaat taaacggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga
    20221 aaaaaaagat tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagcg
    20281 atccgtcggg tcaaattgga attgaagcca ggatcgatcg actatgcaga gtggatcagt
    20341 cgtcatgaga aaccagattt gaaagagcaa aggaaagaag cacaaggatt tgcgtaccgt
    20401 ccattgattt ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc
    20461 attgattcgg tactggatca aacgtatgat cattgggaac tatgtatcag tgatgatgct
    20521 tctactgatc cagctatccg aaagtgtctg gaatcctatc aagcaaaaga tgatagaatc
    20581 aaagtcgttt tccgaaaaga aaatggacac atcagtctgg caactaattc tgcattggaa
    20641 atagcagaag gagaatttat agcactctta gataatgatg atgaattacc gccatttgca
    20701 ttatatgaag tggcaaaagt gctaaacgta catccagaac tggatgtgat ctacagtgac
    20761 gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc agactggtcg
    20821 cctgatacat tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagcatt
    20881 gttaaagaac ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta tgatttggtc
    20941 cttcgtgtga cagaacaaat ccctgcagat catatttacc acattgaccg agtcttgtat
    21001 cattggcgga cgatcccagg atcaactgca agtaatggcg aagccaaaag ttatatctat
    21061 gattccggag tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa aggaagcgta
    21121 cgtcctggca gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg
    21181 gtcagtgtga tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg
    21241 atcatcgaaa aaacgtctta ttcaaattat gaaatcatta ttgcggataa cggaagtaca
    21301 gatccaaaaa tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgctttatc
    21361 gtagaattga ttgacattcc attcaattat tctcgaatta acaatttagc agcagaaaaa
    21421 gcaaacggca aatacttttt attcttgaat aacgatacag aagtgattga gccagattgg
    21481 atgacggtga tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg
    21541 ttttatccag atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca
    21601 gggcatgcat tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt
    21661 gacgtcaatt acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat
    21721 gctgttaacg ggtttgatga aacattagaa gtagcattca acgatgttga cttgtgcttg
    21781 aaagtttatg aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc
    21841 gaatctaaat caagaggtta tgaagatact ccagaaaaac aaagacgttt tgctggtgaa
    21901 atcaaaaaaa tgcaagataa atggccagca tatattgcgc atgatccatt ttacaatgat
    21961 aatttaacaa aagacggaat cggcgacttt tcgttgagac cagattaaac tataataaaa
    22021 agctgttatg ttgtgattcg ctcgcacata acggcttttt atagtttttt gtcaaaaaaa
    22081 ggcagaaggt gccgactcaa aaaaataagt cgaggttggg acagaagtgt ttaactccga
    22141 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ggcttattta
    22201 agaagaagtt gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt
    22261 tcttactttt gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag
    22321 ccaaagtttc acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt
    22381 ttaaaataaa tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt
    22441 taaaataaga agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc
    22501 cactattacc aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc
    22561 catatctcat attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta
    22621 tccacatggg tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac
    22681 caatttttgc cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta
    22741 gtggatcaca aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt
    22801 cttcagctgt ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat
    22861 acttattggc aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata
    22921 tgtgtaaaca gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata
    22981 aatctagtga tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt
    23041 tacatttgta gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt
    23101 tctccccgcg tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt
    23161 tgattaatcc ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg
    23221 acatgatatg acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta
    23281 ccccatcctt atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat
    23341 gcaactcttg tttattcata agatattacc tcactttctt ctataagcat aatgtcatta
    23401 aaaatcgatt tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca
    23461 gcgctttggg aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa
    23521 aaagcagcaa atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg
    23581 gagatt
//
LOCUS       DACDHJ010000025        23294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDHJ010000025 DACDHJ010000000
VERSION     DACDHJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.025"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(166..2511)
                     /locus_tag="IV223_000766"
     CDS             complement(166..2511)
                     /locus_tag="IV223_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8711073.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            2790..3692
                     /locus_tag="IV223_000767"
     CDS             2790..3692
                     /locus_tag="IV223_000767"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8711074.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            3905..5680
                     /locus_tag="IV223_000768"
     CDS             3905..5680
                     /locus_tag="IV223_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8711075.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5746..7332)
                     /locus_tag="IV223_000769"
     CDS             complement(5746..7332)
                     /locus_tag="IV223_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8711076.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7583..7807
                     /locus_tag="IV223_000770"
     CDS             7583..7807
                     /locus_tag="IV223_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8711077.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7949..9544
                     /locus_tag="IV223_000771"
                     /pseudo
     CDS             7949..9544
                     /locus_tag="IV223_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            9571..10410
                     /locus_tag="IV223_000772"
     CDS             9571..10410
                     /locus_tag="IV223_000772"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8711078.1"
                     /translation="MSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITSGKIEFRN
                     VSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEGQILIDDH
                     DIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFVQANKFID
                     ELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTETEGLIQEG
                     LAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYADMYKLQS
                     SGI"
     gene            10736..11587
                     /locus_tag="IV223_000773"
     CDS             10736..11587
                     /locus_tag="IV223_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8711079.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            11684..13564
                     /locus_tag="IV223_000774"
     CDS             11684..13564
                     /locus_tag="IV223_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8711080.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(13604..14506)
                     /locus_tag="IV223_000775"
     CDS             complement(13604..14506)
                     /locus_tag="IV223_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8711081.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            14737..16140
                     /locus_tag="IV223_000776"
     CDS             14737..16140
                     /locus_tag="IV223_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8711082.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            16140..17573
                     /gene="ascB"
                     /locus_tag="IV223_000777"
     CDS             16140..17573
                     /gene="ascB"
                     /locus_tag="IV223_000777"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8711083.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            17718..18644
                     /gene="trxB"
                     /locus_tag="IV223_000778"
     CDS             17718..18644
                     /gene="trxB"
                     /locus_tag="IV223_000778"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8711084.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            18858..20585
                     /locus_tag="IV223_000779"
     CDS             18858..20585
                     /locus_tag="IV223_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8711085.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            20657..20950
                     /locus_tag="IV223_000780"
     CDS             20657..20950
                     /locus_tag="IV223_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8711086.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(21042..21902)
                     /locus_tag="IV223_000781"
     CDS             complement(21042..21902)
                     /locus_tag="IV223_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8711087.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            21994..22422
                     /locus_tag="IV223_000782"
     CDS             21994..22422
                     /locus_tag="IV223_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8711088.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            22584..22760
                     /locus_tag="IV223_000783"
     CDS             22584..22760
                     /locus_tag="IV223_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8711089.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            22787..23233
                     /locus_tag="IV223_000784"
     CDS             22787..23233
                     /locus_tag="IV223_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8711090.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7435 a         4121 c         4961 g         6777 t
ORIGIN      
        1 tccttttttt gtgtcttaaa aaagagagaa agtggttgaa attatattta tcgtatataa
       61 aacaatccgt gtatagatgc tgtattttgt tcaccatcat caaaaaaata agggaatgtg
      121 acaatactat tgtcacattc ccttattact gtgtattcta ttaaatcaat catctttctc
      181 atctgattct ttggttgctt gattcgctct tgcataagct gaagcggtgt tccagtaatc
      241 tgtatagttt tcatcggtac cgccaacgcc aaacttaaag gcgcttttct tcggtccatt
      301 tttcgcccat agcgattcaa ctgtttttcc tggtgtttgg atggattttt tattgacagt
      361 aacttttcca ggtgtttgac cagtaaatcc agatactttt ttcttcatca catcagatga
      421 taattcgaat ttctcatcag tgccaaaaat gtcaggtttt gcttggtaag cttgattgat
      481 cagattggca agataattag ccgaacgtat ccttgctttg ctgtccattc cagtattgtc
      541 atcgtgccct gcccagctac tgatggtaat agacggagta gagacaatta gccatgaatc
      601 tcggtattca ttggtagaac cagtttttcc gacccaatct gcttttccta gatttccgtt
      661 taagcttgag atggcatctt tgaatggtgt agtgattttt gcattgatca cactgcgcat
      721 catgtcattc atgatcgatg ccgttgcttc cgaatagatc cggacagagt tgctttcgtg
      781 tttataaatt acatttcctg cgttatctgt gatggaatcg atgatatagc cttcttcata
      841 gacgccgccg tttgctaaag cctgaaatcc gttggtctgt tgaagcgtcg tgacatctac
      901 tgtacctaaa ggagcagatt caacgcccca attgtcattt gccgggtagt tcatctttga
      961 caagtagtgc tcgtaagcat attgtttcga tccaccggaa ttcaacacat cttgatataa
     1021 gtgataagcc gggatattgt tggaccattc tagtgactca cggacggttt ggaaggtgtt
     1081 cgatccttcg ttagtagcat tgacgatttt ctcgccagca ttttctcctt cttgccaagt
     1141 tgttgcataa tctgatacac gggattctga tccgataagc cccatgtcga ttgcaggtcc
     1201 gtaaacaaga actggtttga ttgatgagcc agcttgacgt tctgtatcga aggcatggtt
     1261 attttgattc tgactgtaat tccgtccgcc aacgaatccg tagatgcggc cagttctgtt
     1321 atccatcaat acatttccgg tttctacttg actgccagca ccatcatcta acatgtatcc
     1381 ataattttca acgccatttt gcaatgcagc atagacatct ttatcgattg tcgaatggat
     1441 tgtatatcct ttattttgga tcgtttgctg tgccttttga taataagcgt cactgatcga
     1501 tgaatcagac atatcagcgt tgtctttttc agccaattgt tgtgcaatga ttcttgttgc
     1561 ttcattcatc acagtataat acaagaattc tctgtcagtc tgttctgcga tttgttgtgg
     1621 tagaaagtct tttgtaaggt cataggcttt tgcttcttcg tattgttctt tcgtaatttg
     1681 gccttcacga tacatactga ataaaacaaa atcttttctg gcaaggccgg cagaaagatc
     1741 ttcttttagc gcacctgtgt tcgtgtatgg actatacgtg atcgggcttt ggggtaatcc
     1801 agcgatataa gcagcttgcg gcagagtaac atcttttgca tctacaccaa agattcctct
     1861 tgcagcttcc tggactccag caatgttttg tcccttatta ttacgaccaa atggtgagac
     1921 attcaaatac gtagcaatga tctcatcttt tgaaaagtat ttttctactt gtgcagaaag
     1981 aatgatctcg tttgctttac gtttgaatgt tgtttcatct gtaaggatct gctgtttgac
     2041 taattgctgt gtcaaagttg atccaccaga agagccaata ccagtagctt cagatactaa
     2101 tgctcgtaaa acagctttag gaacgtaacc ttggtgttta tcgaagtatt catcctctgt
     2161 gctgataatt gcagttttca ataaaggtga aattttatca gatgaaatag tcgtgcgcat
     2221 cagatctgtc tggatttttg atatctctgt attatctgca taaacaatct ttgacgtttc
     2281 tgtaatgttt cctagttctg tctgcagtgc ttttttagtc ggcaattgtg tgtcttcgac
     2341 gagataggca aaatagccag cgcctattcc aatacctaga gctgcgaaca tcaaaagaag
     2401 aacagtaata aaaacagtca atgattgaaa aactctaaaa ataatcttag gaacaaacca
     2461 tttttctttt tttcttctat tctgtttttt tcgagtattt ttgcgtggca aaagggtcac
     2521 ctcaactaat ggttttattt cgctaattat agcaaataat agaacaaaaa aacagacaag
     2581 gctgtttttg gcaggaattt cttttttaaa ggagttaaaa cgtgagaaaa tgatcttttt
     2641 tataaaagag ttcttaagct tttctttaga gaaaatttca gttactatgg agaacagggc
     2701 aaagaaaaat caatcatttt ctttgtatat catcttacca atatatagga attgttagta
     2761 acaattgcct ttcaaggagg aatccatcca tgttattagg ctcacacgtc agcatgagcg
     2821 gaaagaaaat gctgttagga gcagcagaag aagcagccag ttatggatct tcgactttta
     2881 tgatctatac aggcgcccct caaaacacta gaagaaaagc catcgaagag atgaatattg
     2941 atgccggaaa agcgtacatg gcagaaaacg gtctaacaaa tatcgtcgtt catgcccctt
     3001 atatcattaa tttggggaat acgatcaaac cagaaatgtt tccttttgcc gtccaatttc
     3061 tgagagaaga gattgttcgt gcggaagctt taggcgctac tcaaattaca cttcacccag
     3121 gtgctcatgt aggggcagga acagcagccg gaattgctag aattattgaa ggcttgaatg
     3181 aagttttgac aaaagaacag acggcacaaa tcgctttgga gacgatggca ggtaaaggta
     3241 cggaaatcgg tcgaacattt gaggaattag cgcaaatcat cgatggagta aaattgaatg
     3301 aaaaattatc cgttactttt gacacctgtc atacgaatga tgccggttat aatattaaag
     3361 aagacttttc aggtgtaatg gaagagtttg ataagatcat cgggttagat cggcttaaag
     3421 tactccatat caatgattct aaaaatccgc aaggttcgca taaagaccgg cacgccaata
     3481 ttggttttgg aacaatcgga ttcgacacgt tgaataagat cgtccatgat ccaatgtttg
     3541 acaatatttc gaaaatcttg gaaactccct atgtcggcaa agataaaaag aaagcttacg
     3601 caccatatgg aaaagaaatc gccatgttca aagcgcaaac attcgatcca gaagtgtttc
     3661 ctgagttatt agcagaagaa gtgatactct aagattttct atttgacagc agcaaaaaag
     3721 gaccaggcac attgccttgt ccttttttgt ttaagaaaca aaggagtact ttttgcagtc
     3781 tttttcatca tgcatcttct cccccctctt tttaaattta gacaaacagg gtatactggt
     3841 agaaaagtaa aaaaacgaaa agtttcacaa gcaagtggca aaacgtgatt ggaaggacga
     3901 aaagatgcta aacactttaa aacccttgat caaccaactg actaagaaac atacatactt
     3961 tattcttggc atagccctat tatttacaat tattcgtttg tatttatata gtaaagcaat
     4021 ctatggaaca gacatgtcgg caacctatga tgatcagttg ctgattcgct atgctcaaaa
     4081 tattttggac ggtaaatggc taggggagta tacgactaca acactttcca aaggaatctc
     4141 ctacagctta tttttagtac ttgcacataa actgcatttt tcttatgcag ttttattcgg
     4201 catgttcagt atttttgcca gtatattggt tgcactcgcg tttaaaccga tcgtgaagaa
     4261 caagtatctt cttttaagta tctattttta ttttttgttt tctccgatta gttttacaca
     4321 tgaatattcc actcgaacgt atcgtaatgc cctcgtgatt ccggctgttg agatcattat
     4381 cgcttgttta ttagctattt attaccgcaa atacggttct agcagaaaat tgctgccatg
     4441 gtttttaggg ctttctgtcg cttttccttt tttttggttc attcgcgaag attctatctg
     4501 gcttcttcct tttactgtag tagctttggg tatcagtttc gtccagatct tgctagggca
     4561 agacttttct ttcaaagaag taaagaagtt aaaaaaacga ctctttcttc tctcgaaacg
     4621 ggaatggctg aaactttcat tgtttttact tcctatagtt ggcttttcca tcgtctatca
     4681 gtggatcgaa tataaaaatg agacaacata cggtattgca accatcaatg accgttcgag
     4741 tggagaattt ggacgtttga tgaaacaact gatccgaata gatgatgaaa cgaacttgaa
     4801 taaagaaaac agtgagatat gggtctcaca tgctgcacta aataaagcat tagccgcatc
     4861 tcctacattc catactgttt ctacccgtat tgacgaatta tatacgactc acgcgtggac
     4921 tcaaggtgga aaagtaaaag aattacccgg agatatcatc ttttgggcat tgcgagatgt
     4981 agtaagcgaa tctggttatt accaaaacaa tgcaaaagaa accaatgatt tttggaaaaa
     5041 agtcaaccaa gaattagaag aagcctatca caagggaacc atcaaaaaga aaaaagagtt
     5101 ctatctgatg ggatcaggag atggaaagac gatgagtgat ctgccaatcg tattcgattt
     5161 ccttcaatca ggatacgctt atgcatggcg atataaagat ttccatcaag gaggaaatta
     5221 tagtttcgga acgcaagaac aagtcaaaca ggctcaagaa ttattaaacc ttccgctaat
     5281 ggagaattgg atcgatagta atgaaccgag aacacctaac ttccaattaa caaaatcagc
     5341 aaaagtcgcg aatgtcatca taaaaattta ccaagttctg gctattcccg tgctgattgc
     5401 ttcaatagca gggatattac ttatgatact aggaaccgta tttgcaaaag ctgatcgtgc
     5461 ctattatagt tcctctttga ttgtggtcac cggattaata ctgacacaat tggtttttct
     5521 tttcggtgtc tcatggtttt gttcttatgc gccagaaaaa aaggactatt ttttaagtgt
     5581 ctacactggt gcaggcgtgc cattaacgca attcatcgta gtaacaagca tgctagtttt
     5641 cagtaaatta aatctgaaaa aagctttatc ttctaaataa aaagaccctt ggtagctaga
     5701 tttttaacct aactatcaag ggtctttgtg tggaaaaatc agtttttatt cgatttcttc
     5761 taccgtttcc tgcaaaagct gttcatattt tttgtttcct ttatataaat caccaatcgt
     5821 ccatgccaac aggagaagga caagaacaat gattacataa gcgatgtgat cagccaatgc
     5881 cagttgagcg gaagtggctt gctcgccgaa gaaactttcg atctgtcctg gtaatcctcg
     5941 taagttcaag taagttagac taatcacaga gaaccagcca agaagtttta cgatgaatga
     6001 atttttaaat cggctaccca tctccgcttg gctgttggtc atcatcagta aaggaatcat
     6061 cgaaaatggc aacgcaaaag ccaagaacac ctgggattca ttcattaagt tattcaaagc
     6121 ggtatgttct tcgatcgttc cttttgtact tgtaaacaaa acacaaatca atacaggaat
     6181 cacagaaagc aatcgtgtga ccagtcttct tagccaaatc gggatacgca tatggatgaa
     6241 tccttccatg atgacttgac cggtcaacgt accagtaata gtagagttct ggccagaagc
     6301 aagaagtgct acagcaaata aagtggacaa gataccagat tttgctacag caatcaaaat
     6361 accattgctc atagctgatg gatcagataa agcttcatac agaccaaaaa aggaaggatc
     6421 ttggacagaa ccggttttga agacagcgac acccatgatc aatagcagtg cattgactag
     6481 aaaagccatt gacaactgga tattagaatc ccaagtagaa aaacgtacag cttgtgctac
     6541 atcttcttcg ttttggtgat cgatcttccg agtttgtgaa actgctgaat gaagatataa
     6601 gttatgcggc atgactgttg caccaataat ccctaaagct ccagttagcg gagtttcgcc
     6661 accaatgaca ggtgagtctg agaatgtatg gctgttaggg ataaagccag caaataaact
     6721 gtgccaatct gggttagaca agccaacttg atagacaaaa acgaataaaa tgacgaaaat
     6781 taagcagaca accagtgctt cgatcttacg aaacccgact tttgtcagta gtaagagaac
     6841 gaaaacatct aatacagtaa taaagacggc aatcaccaat ggaatatcaa aaagtaagta
     6901 gagggcgatt gctgcaccaa ttacttctgc aatatctgtt gccataatgg ctaattcagt
     6961 caagatccat aaaatgacac ctaatgtctt acttgtgcgt gctcggattg cctgtgccaa
     7021 atccatttgg ctcacgattc ctaattttgc tgccatatac tgtaaaagca tagcaataag
     7081 actagacatc aaaataactg acatcaataa atattggaag ttttgtcctc cggtgatcga
     7141 agtagaccag ttacccggat ccatataccc aactgctact aacgcaccag gtccagagta
     7201 agcaagaagt gttttaaaaa aacttttccc ctttggtaca tcaactgttc catttacttc
     7261 agctaaagat aaaccatttg tatgttcaat aagtcgatgc ttatgaaatt gtttattgga
     7321 ttgattattc aagaaaaaac cacctttcct atttatctac gattacattc tacacctcaa
     7381 aaagaaaaaa ttaaagaaaa agattcggta tgcctaattt attttcgtgg cgtgtcttgc
     7441 tagatgacga aagaaatttt gttctcctct aaaaaaaccg ctattttttg catgaaacta
     7501 aagaatacgc ctttcttttt tcaataaact ttagtaaaat aagtcattga gagcaattcg
     7561 gaaatagaaa ggagagattt atatgttatc aacaggtatt gtcgtactta ttgctattat
     7621 tgctttgcta atcggcgcag taggtggctt tttcctagcg cgtaaatata tgaaagacta
     7681 tttagaaaaa aatcctcccg ttaatgagga catgttgcga tcaatgatga tgtcaatggg
     7741 gcaaaaacca tctgagaaga aaattcgcca gatgatgcag caaatgaaga accaagggaa
     7801 aaaataggtt ctgacccaac gactttttac gggctgattt tttcagcccg ttttttattt
     7861 cgtaatttgt tagactattc agattattca gcaaaaaaga caagcacaga caatcctagg
     7921 ctcttgataa agacaaagga ggagtaaaat gtcgattttt agaaaattag gctggttttt
     7981 tcgtcaggaa aagaaacatt atttaattgg agttttctcg ctattcgcag tggccttggt
     8041 acagttagtt cctccaaaag tgattgggat catcattgat gagattgccg ataaaaattt
     8101 atcaataaag atcattttcg ggtggatctt cctattgatt atcgctgctt ttttacaata
     8161 tctttttcgt tatatctggc ggacgaatat ttggggaagt gcagctcggt tggagaaaga
     8221 acttcggcag cagttattca aacattttac agaaatggac aatctctttt accaaaaata
     8281 tcggactggg gatttgatgg ctcatgcaac gaatgatttg aatgcgatcc agaatgtagc
     8341 aggagctggt atcttgacct ttgcagattc agtgattacg ggcggaacaa cgatcattgc
     8401 gatgattttg tttattgatt ggcgcttaac actgattgcg atgattcctc tgcctttgct
     8461 ggccatcacc tcacgagtgc tgggatcgaa actccatgat gcttttcgtg attcgcaaga
     8521 agctttttct agtatcaatg ataagacaca agaaagtatc actgggatca aagtcattaa
     8581 aacatttgga caagaaaaag aagatctagc ggattttgaa gaaaagattg aagatgcaat
     8641 tattaaaaac aaacgagtca attttttaga ctccttattt gatccattca ttaccttgat
     8701 tatcggtctg tcttatgttg caacaattat tttaggtgga aagtatgtct tggaaggcac
     8761 gatctcgatc gggcagatgg tctcatttat tagttatatc ggtatgcttg tttggccgat
     8821 gtttgcgatt ggtcgattgt tcaacgtatt ggaaagagga aatgcttctt atgatcgcgt
     8881 caatgaactt ttgcatgaaa aaacgcatat cgtggaacaa ctgggtgcag tgcagacccc
     8941 ggcaaaaggc gagctgaatg tagctatttc ctcttttaat tatctgaaag aagaatcacc
     9001 agcattagaa caaatcaagt ttgcaatcca agaaggagaa acattaggta ttgttgggaa
     9061 aactggtgcg ggaaaaacga cgatcatcaa attactgatg cgtgaatacg atgattatca
     9121 agggaaaatc caatttggcg gacgttcgat caaagaatat tcattgaatg ctttattagg
     9181 atcaatcggc tatgtgcctc aggatcactt cttgttttcg atgaccattc gagataatat
     9241 ccgtttttct aatccaaaac tttcagaaga agaagtaaag catgcggctt ctttagcatt
     9301 tatcaacaat gaaatcaagg aattaccaaa aggttatgat accttagttg gagaacgtgg
     9361 agtctcttta tctggcggac agaaacaaag gatttcgatt gctcgagcat tgatcaaaaa
     9421 tcctgaatta ttaatgttag atgatgcact ttctgcagta gatgccaaaa cagaagaagc
     9481 tatcttgcat aatttaaaag aaataagaaa agagaaaaca actatcattg cagctcaccg
     9541 tttaatccaa tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct
     9601 ggtagtcgta tcttgaagat tatggataca gaagaatatg caccacaaca aaaccctgga
     9661 gcagatcaaa tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat
     9721 ggagagcaca atgtattaaa caatatctct tttgttgcta atccgggaga gaccgttgca
     9781 ttagtgggac acaccggaag tgggaaaagc tcgatcatca atgtgttgat gcggttttac
     9841 gaattttatg aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa
     9901 gagttacgta agaaaatggg gatggtcttg caagatgctt tcttgtttta tggggatatc
     9961 gcaggaaata ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct
    10021 gagtttgttc aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg
    10081 atcgaacatg gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca
    10141 atcgtgactg atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa
    10201 acagaaggat tgatccaaga aggattggcg aaaatgcgac aaggacggac aacgattgcg
    10261 attgcccatc ggctgtcaac gattcgagat gcagaattga ttttagtttt agacaaaggt
    10321 catatagtag aacacgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat
    10381 atgtacaaac tgcaaagcag tggtatataa aagatgttgt gacagtagca ttttgaccta
    10441 agcactagag tgaaaataaa acatagcttc ccatattttt catctgtgag agttagtgtc
    10501 gtagttacca gatttaaaaa agacgacgtc agctagtcag aagtgcctaa tagcccctaa
    10561 gaaagcgttg acaaaatcgt ttgctttcga taatacaaag aaaacgatac aacttggata
    10621 gtgattgcct aatgcaagct aaacctgatg agtctcccat ggtttcatgg acggcttttt
    10681 gggtttttct ttttgttttg gatacagccc tatatatcat ggaaaggagc aaaacatgct
    10741 gattgagaaa gtactgaata acaacgtcgt aataaccaga gatgaaagaa atcaggaaat
    10801 gatcgtgatg ggaaaaggtt tggcattcaa aaagaaagtg ggtgacccga tttttgactc
    10861 atgcatcgac aaaaaatttc agttgacaaa tgacggactt tcacaacagt tccaagaatt
    10921 ggcacaagcg attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa
    10981 agaggatcta caagtccatt tgaatgattc tgtgtatatt gcattgacgg atcatttgta
    11041 tacagctatc gaacgtcaaa aaatggggat ctttgtgcca aatgtattgc tgtatgatat
    11101 caaacaactg tttcctaaag agtatgccat tgggaaaaaa acagtgaaga aaatctcgga
    11161 taaataccac atcaagttat cagaagacga agccggattt atcgcattgc atttagtcaa
    11221 tgcacaaggc attcaaagcc agcaagacaa tacctacctg tttaccacaa caatacagga
    11281 tattttaaat atcgtccgct attactataa gattacattt gatgaagatt caatccattt
    11341 ttttcgattc gtcacacacc tacgtttttt tctgacacgc gtcctagatg gagagaaatc
    11401 agaagtggga gaagtttctg aggaattgtt ccaggttatt caaaaaaaat ataaggacac
    11461 gtttagttgc attgagaaaa tagcagactt tttaaccggt gctttcgact accagatgtc
    11521 cacggatgaa aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg
    11581 taaataaata aaaaaggagg acaaattaac aagaacgtat gaaaataagg gtagtgacat
    11641 tcagttggct aaaccttcca ctaattaatt ggagggaata gaaatgggaa aataccagga
    11701 attagcagaa aacatcgtaa agaatatcgg aggaaaagaa aatatccgta gcttgacaca
    11761 ctgtgtgacg cgacttcgtt tcaaactaaa ggaggaaaat aaagcaaagg atgatgtttt
    11821 gaaaaagatg gaagggatag tcaccatcat gaaaagcggc gggcaatatc aagttgtaat
    11881 cgggaatcat gtgccagatg tctatgcaga agtactgaca gtagccggga tttccgatga
    11941 agcagaagaa gaggtttctt caagtagtaa tctgttcaat cgattaatcg acatattaag
    12001 tggctgtttc cagccttttt taggtgcttt ggctgcagcg ggaatggtca aaggattaaa
    12061 tgctttgttc ttgtttctca aattttatac tgaaggatcg ggtacgtaca tcatgctgaa
    12121 tgggataggg gatgcaattt tttatttcat gccagtgatc ttagggtaca cagcagcgaa
    12181 aaaatttcat ctgaatccga tggtggggct cgtgattggt gcagcacttt gttatccgac
    12241 tgtacaagga gcagcactac aaagtggttt ccctgatggc agtcctgcgc cttatagctt
    12301 gctgggtctt ccggcttatg aaacattttt gggtatccca tgggtgggca gcagttatac
    12361 cagtagcgtc gtacctgtta tcttcattat cgcatttgcc ggacaagtcc aaaaaattgc
    12421 caagaaatgg attcctgcag tcgtccagac ctttttggtt cctttttctg tgttattaat
    12481 cgcacttcct gctggctttt tgattatcgg tccgattgtc agtatactga cagatctatt
    12541 aagcacggga ttccaagcat tgatggactt ttctccagct gtgtatggtg cagttctagg
    12601 gtttctttgg caagtactgg tgattttcgg acttcattgg agtattatcc ctcttgcgat
    12661 tatgcagcta gcgcaaaatg ggtggagtca gatgttagta ggcgtttatg gcgcgagttt
    12721 tgcacagaca gcagtggtat tggcgatgta tttcaagttc aaggatcaaa aaatgaaggc
    12781 gctttgcatg ccagctgtga tttcaggaat cttcggcgta acagaaacgg ctatttatgg
    12841 aatcacctta ccaaagaaga agccatttat ttactcgatg attggtggtg ctgtttcagg
    12901 aatctatttg atggcatctg gaacaatcag ctacacaatg ggcgggttag ggatttttgg
    12961 ggtggtaaac tacatcacgg aaacagacgc cagtggtatg tttcaagcgt ttatcggcat
    13021 tgctattgct tcggtaatcg ggttttcgct gacttttttc ttttggaagg atgaagcagg
    13081 tgaaacagat gatatacaga aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg
    13141 acaaatcgct ccactaaatg cagcaaagga tgcagctttt gcacaaggaa cactaggtcg
    13201 aggaatcttg atctaccctg aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat
    13261 gaccctgttc ccgacgaaac atgcaattgg aatggtttct gaaacagggc tggaattgct
    13321 gattcatgtc ggattggata ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca
    13381 gcaaggagaa agagtgaaac aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga
    13441 aaaggaagga tacagtttag aaacacctgt gatcgtcacc aattacacgg attatttgga
    13501 tattttggaa aaccaagggg agacggtcgg gccagacgat gcacttatca ccgcactgac
    13561 ctgaacggtt aggtgaagaa tggactaggc ttaactattc tttttattcc acttctttat
    13621 tttcaaaatt gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcgaattttt
    13681 gaccgacaga aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat
    13741 ataacacgtc tagaagataa caaattgatg tattgatcgt aaaattacca atttttgaat
    13801 acagtcgctc gcgtgtcgta agtcgcaaag cggctgtact cattgcagat agcgttgaat
    13861 caccgatact ggtcaaagct aaaattggaa catcattttg tttcaatatt tctgcggtac
    13921 gaaggatcac attgttttca cccgtataag aaatcagaat cccgcacata tcactcgtgc
    13981 aattgtacgc ttcatagaac gattcaccaa aagtggtgac aatcgtgaca tccattttga
    14041 tgcgcttcat tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa
    14101 tcacgatttt ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa
    14161 tgagagaaag tgtatcatga atcgtcatct gttctaaaga agccatttta tttgcgatcg
    14221 tcataatacc gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt
    14281 caagataggc tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca
    14341 ttttttttgc tacacggatg agtgtagacg ggtggacata gcatcgctca gcgatctctt
    14401 ggatggtcat ttcgccaagt gctggagaat cgcttaagag aaactggatc acttcatttt
    14461 ctgaaggaga aaaggagatt tctttgattt tttcacgtaa taacatgtag ttaccttctt
    14521 cttatttttt tacgtaaatt ataacttttt tgaaaatgtt ttgtaaacgg atttatacta
    14581 tataacttaa aatagacttt ttttgacaag atatttacaa aaatccacta gaaaacagta
    14641 acttttgtaa atgttactgg aaagaaacgg atttctattg tttgttttgt ttacaaaaaa
    14701 gataatgaaa gtgtaaaaaa gtaaaggagt gagaacatgg caaaaggaat caaaatcgct
    14761 acgattggtg gaggcagcag ttacacccct gagttgatgg aaggatttat caaaagatat
    14821 gaggaattac cgatacgaga aatctggctc gtagatgtag aagaaggaaa agaaaagctt
    14881 gagatcgtta gtcagatgtc tcagcgtatg tgggatgctt ctccgtatga agtcaaaatc
    14941 tatgcaactc tggatcggga agaagcattg aaagatgcgg attttgttac gactcagttt
    15001 cgtgtcggtc aattagatgc acgaatcaaa gacgagcgga tccctagtta ttatgggatg
    15061 attgggcaag aaacaaatgg tgctggcggg atattcaaag cttttcgtac cgttccagtg
    15121 gtcttggcaa tcgttgaaga catgaagcgt ttatgtccaa atgcttggct gatcaatttc
    15181 gcgaacccaa gtgggatggt gacagaagca gttatcagat atggcaaatg ggataaagtg
    15241 attggattat gcaatgtgcc agtaatggcg atgatgacag aaccagcaat gcttggcaag
    15301 aaaaaagaag agctgatcta caagtttgcc ggtctaaatc atttccattg gcacaaagta
    15361 gcagataata aaggaaatga ccgcacggca gaattgattg atcgtttata cgatgaagac
    15421 aatggattgc cgaaaaatat ctttgatatc ccgtttttca aagaacaatt aaaacaaatg
    15481 aatatgatcc cttgtggcta ccaccgctat tactaccgtg aagaagaaat gctggaacat
    15541 gctttagaag aatatcaaac gattggtaca agagcgcagc aagtgaaaga aacagaagca
    15601 gagctatttg aattatacaa agatccagaa ttagaccata aacctgaaca attgcaaaaa
    15661 cgaggtggcg cttattattc agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat
    15721 aaagaaacac agatcgtggt atctacaaag aatgatggag ctgttcctga cctgccggca
    15781 gattgtgtag tagaagtcac tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt
    15841 ggttctcttc ctgctgctga aagaggctgg ttgcaagtaa tgaagaatat ggaattactg
    15901 acgatcgaag cagctgtgac tggtgattat gggacagctt tacaggcgtt tacgatcaat
    15961 ccgcttatac gatcaggaaa gacagcaaaa cggatcatgg atgagttgtt tattgcacac
    16021 cagcagtatt taccgcaatt caaggaaaca atcagccgat taaaagaaga aggaatcgaa
    16081 gttcaagatg aactggcaag agaattatca ggtaatacac atataatgga ggaagtataa
    16141 tgggattcag aaaagatttt ttatggggcg gtgctacagc agccaatcaa tgtgagggcg
    16201 gttatgatga aggtggacgc ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc
    16261 gttttcctgt tatcaccgga aagaaaaaaa tgtttgaatt tgatgaggat catttttatc
    16321 cagcaaaaat tgcaatcgat atgtatcatc gttataaaga agatattgct ttgtttgcag
    16381 aaatggggtt caagacttat cgaatgtcca tcgcctggag tcgtattttt ccaatggggg
    16441 atgaaaaaga gccgaatgaa gctggcttga aattttatga ggatgtcttt aaagaatgcc
    16501 aaaagcacgg aatcgagcca cttgtgacga ttacgcattt tgattgtcca atgcatttga
    16561 tcaaagaata cggtgcgtgg cgttccagag aactcgttgg tttttatgaa aatctgtgtc
    16621 gagtcatctt caatcgatat aaaggacttg tcaaatactg gctgacattc aatgagatca
    16681 atatgatcct tcatgcacca ttcatgggag caggactgta ctttgaagaa ggagaaaacg
    16741 aagaacaagt caaatatcaa gcggcacatc atgagttggt tgcaagtgcc atcgcaacga
    16801 agattgctca tgaagtagat ccggagaaca aagtaggctg tatgctggca gcaggagcat
    16861 attatccata cacttgcaat cctgaagatg tttgggcttc tagaaaatca gatcgagaaa
    16921 attatttctt cattgatgtt cagtcacgag gagaataccc agcttatgca ttgaaagaaa
    16981 tggaacgaaa tggcattcag atcgaaatgg aaccagggga tgccgcattg ctgaaagaac
    17041 atactgttga ttttatctct ttttcttatt attcttcccg agtttcgact accgacaacg
    17101 aactcttgga acagactgca ggaaatatct ttgcttctgt caaaaatcca tatctgcaag
    17161 caagtgaatg ggggtggcaa attgatccat taggcttgag aataacgatg aatgatattt
    17221 atgatcgtta tcaaaaacca ttatttattg tagaaaacgg cttaggtgca gtcgatacgc
    17281 cagatgagaa tggttatgtg gaagatgatt accgaatcga ttacctggca gcgcatatcc
    17341 aggcgatgaa ggatgcagta gaactagacg gagtcgatct attaggctat acgacttggg
    17401 gatgtattga tttagtatca gctggtaccg gtgagatgaa gaaaagatac ggattcattt
    17461 atgtcgatcg tgataaccaa ggaaatggca cattagcccg ttcaaagaaa aaatcattca
    17521 actggtataa aaaagtaatt gattcaaatg gagaaaatct ttcgaacgat tgatcaatga
    17581 tttataaaag tctattgttt ttcagcggat ttttcgagta taataaagct attcgagaaa
    17641 cgcaagcaga acaatagact ttttttcgca ttggctgtaa agtatgctta cttgattaag
    17701 taaaggagaa tctacatatg tatgatgtaa tcgttattgg cgcaggtcct gctgggatga
    17761 ctgctgcttt atatgcttca cgttcaaact tatccgtatt gatgatcgaa caaggtgccc
    17821 ctggcggcca aatgaacaat acggcagaaa tcgaaaatta tcctggattc gattcgatca
    17881 tgggccccga tctagcttat aagatgtatg aaagtgtctc aagatttggg acagaaaatg
    17941 cttatggaat cgttcaagat atcaaggatc acggcagcca taaagaagtc atctgtgaag
    18001 ataaaagtta cgaaggaaaa acagtcatta tcgcaacagg ctgtatccac cgtaaattag
    18061 gcgtacctgg cgaggaagaa tttgctggac gaggcgtttc ttactgtgca gtctgtgatg
    18121 gtgcgttttt ccgtaacaaa cgtctagtag tcatcggggg tggggactca gcagtagaag
    18181 aagccatcta tttgacacaa tttgcttctg aagtagtgat cgtccaccgt agagatgaat
    18241 tacgtgcaca aaagatcatc caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt
    18301 gggatacagt cgttgacgaa atcgttggaa acgaaatggt agtgacaggt gtgaaagcac
    18361 gtaatgtcaa aaccggcaat gaatttgatt tagcagcaga cggagtattc atctatgttg
    18421 gactggatcc attaactgca ccttttagag aagcaggttt gacgaacgaa gctggctgga
    18481 ttccaacaga caacgaaatg aaaacgaata tgccaggcgt ttttgccatc ggagatgttc
    18541 gtgaaaaaga tttgcgacaa atcactacag ctgttggtga cggcggtatt gctggacaac
    18601 aagtgtataa atacttggaa tctttagaag aaacaactgc ataagtttaa aaagagtaga
    18661 agaacgggtt caattgttct tttactcttt tttttcgcgt gaaatttact gaatctgatt
    18721 tatgcgaaca cgtccaacac cttttgataa aagaatcctt tcttgattta agcgtttttt
    18781 ggtcctcata ctttcatatc taataagtga atgctatact tattaagaac catatttcag
    18841 aaagaggtgt tattttcatg tcttgggaac aagtgtatga acaatgggta catgaagaaa
    18901 atttagaaga aaacttaaaa aaacaattaa atgatctggg tgaagatcct gaaaaacttg
    18961 aagatgcttt ttatgcaccg ttggaattcg gtactgcagg catgcgggga atcttaggac
    19021 ctgggatcaa tcgaatgaac atctatacgg taagacaagc aacagaagga ctagcacgtt
    19081 tcatggatac acaaggtgaa gaagcaaaac gccgaggtgt cgctattgct tatgactcac
    19141 gccatcagtc accagaattt gcaatggaag cagccaaaac actggcacat cacaatatcc
    19201 cgtcttatgt attcgaaagt ctgcgtccaa caccagagct ttcttttgca gttcgtcatt
    19261 tgaagacgtt tgcgggaatc atggtaactg catctcacaa cccagcggct tacaatggat
    19321 ataaagtcta cggagaagat ggcggacaaa tgcctcctgc agacgcagat gcattgacga
    19381 aatatgtccg tgaagtcagc aatccgctaa aagtcgaagt attatctgac gaagaagcta
    19441 aacatagcgg gttgatcacg atcatcggag aagaagtgga cgctgcttat ctagaagaaa
    19501 tcaaagcagt gacgattgat cgtgaattgg tagaaacaat ggggaaagat ttgaaacttg
    19561 tgtacacgcc acttcatgga acagggaaaa tgttgggcga acgtgcgttg aaacaagctg
    19621 gattcgaaca attcgtacta gttcccgaac aagctgtggc agatccagat ttcactacgg
    19681 tcaaatcgcc aaatccagaa gaacattcag cttttgaata tgctatacga cttggagaaa
    19741 aagaaggagc tgacttgctg attgcaacag atcctgatgc agaccgttta ggcgcagcgg
    19801 ttcgtttgcc agatggttct tatgaagtat tgactgggaa ccaaatcggg gcattgtttg
    19861 ctcaatatat tttggaagca catcaatcaa gaggcacttt accagaaaac gcagcggtat
    19921 tgaaatcaat cgtttccagc gaattgccta cagcaattgc ggaacactac ggtgctacca
    19981 tgttcaatgt gttgacaggg tttaaattca ttgctgaaaa aatccaacaa ttcgaagaag
    20041 accattcaca tacttttatg tttggattcg aagaaagtta tggttattta gttaaacctt
    20101 ttgttcgcga taaagacgcg atccaagcat tgcttttatt agcagaagta gcagcttact
    20161 ataaaaaaca aggcaaaacg ttgtatgacg gcttgcaaac gatttttgaa acctatggct
    20221 attttgcaga aaaaacgatt tctgtgacaa tgagcggaca agaaggttct gcgaaaatct
    20281 ctgctttgat ggaaaaattc cgtaaagaag caccgacaga atttgccgga gcaaaagtta
    20341 ttcaaacaga agactttaaa gaattgacga gaacagaccg tgaaggaaac gtagaaaaac
    20401 taacaacacc gccatccgac gtattgaaat atacattgga agaaaatggc tggattgcta
    20461 tccgcccttc aggaactgag ccgaaaatca agttttatat tggtgtaaaa ggaagctcta
    20521 cagaagacgc agccgaaaaa gtagcctctt atgaagcgat tatcaaaaag atgacagaag
    20581 aataaaaaac ttgagactga tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa
    20641 gttggaggtg aatggattgc cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt
    20701 attcaaaatg ttgagtgatc ccactcgtct taaaattctg ctatatctga aagacgggga
    20761 acaaaatgtg acagcaatca cccaagcagt ggaaatggag cagtctgctg tatcgcatca
    20821 actacgttta ttaagagaaa atcatgtcgt caagtctcat cgcgagggaa aagcgatttt
    20881 gtatagtctt gatgattctc atgtactgga tattttgaac cagacactca aacatgtgga
    20941 acatcaataa cgataggtgc aaatagataa acactggagt gtgacaacga aatttcttcg
    21001 ttgtcacact ccagtgtttt ttaagaaaaa gacaaaaatg attaatcgct tttttctgtt
    21061 gtttcagttc cataataact atgatcttct aggtctaaag cattcaaaat aagcgaagcc
    21121 gtttcttcaa tagataaaga tgccacattg atcactacac atcctagttt ttcataaaga
    21181 tcattcgcaa aatcgagttc agctctaatt ttttcgatat cggaataagc agtatcaggg
    21241 tttaatccgt aagagcgcat ccgttcttta cggatattat ttaaaatatc tgggttattc
    21301 gttagtccca cgattttttt aggatcgatc tcccaaagct gtttgggaat atgtgcttgt
    21361 gggatcaaag gtaaattggc aacttttaaa tttttgttag ctaaaaacaa gctcaaaggc
    21421 gttttggaag ttcgggatac gcctagcaaa acgacatctg cttctaaaaa accacgagga
    21481 tctttcccat catcgtattt tacagcaaat tccattgctt tgatccgttt gaaatagttc
    21541 tcattcaata aatggagggc accaggttgc cgaattggct tgataccggt taaacgttca
    21601 atttctgata tcggtggcgt catcacatca aaactaaata attgatgttc ttcacaaaaa
    21661 gaacgtgtta tctcgatcag ttcttgattg attaatgtat gcaggacaag tgctttttct
    21721 tctaatgcat cttgcaatgc ctgctttaat gttttttcat ctgtcacgaa cgttctgcgg
    21781 aataggttga actcaacggt aggatactga gccatagtag cttgcgctag ttttgtcgca
    21841 gtttctccag cagagtcgga gatgacaaaa aaagtaacga tttctgtaga atcggaagac
    21901 atggataaaa cctcacttgc atttttttta tgtttattat accatactct agaatgcgta
    21961 cttcgtaatc attttgattt ggaggaataa gtcatgaaaa aacagacatt gatcgaacaa
    22021 tcattagaaa cattgaaaaa taacggtttt aaatatacta aaaaaagaga agcattgtta
    22081 acttatttag tgaagcgtaa ccgctacgta tctgctaaag aagtttttga ctatatgaat
    22141 caggaattca aaggggtaag ttatgatacg atttatcgga atctccatga ttttgcagag
    22201 atcgatttac tggaagaaac cgatttaaat ggagaaatga agtttcgttt tcattgctgt
    22261 cagggggaaa tcggccatca ccatcatttt atctgtacag tgtgcggaac aacgaaagaa
    22321 atccatatgt gtccgatgaa ttatttcgaa gaacagctag gtggatgcac gatcgaaggg
    22381 catagattcg agattttcgg aagatgtgaa aattgtcaat aagggtttaa ataaaataac
    22441 atttgataat tttaaaaaaa agcaagattt ccttgacggt caaaacagca ttagatataa
    22501 tatttacagg acagtcttgt tttttgagat atatttcaat aacaattacg gttacttttt
    22561 aagctcggag ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg
    22621 acgatgctct tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta caagaatctc
    22681 gtaaacgtga attctacgaa aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta
    22741 gaaaacgcaa aaaattctag ttctgtgatg aattggagtt ggtagtgtgt cactactaag
    22801 tagattaaac gatgacatga aagcagcgat gaaagcgaaa gataaggaat ctttacaagt
    22861 catccgaatg atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc acgacttgac
    22921 agaagaagaa gaactaacgg tgttgtctcg cgaaatgaaa cagcgacgtg attctttgca
    22981 tgagtttgaa gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat
    23041 agtcgaaaaa tatatgccgg aacaactttc cgatgaagaa attcgacaac ttgttcaaga
    23101 agcaatcact caaactggtg cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat
    23161 tatgcctaaa gtcaaaggaa aagcagatgg aaaccaagtg aatgcgattg tgaaagaact
    23221 attacaatct taattttatc atttaaactg cggacagtga ttgtccgcag ttttttgtgt
    23281 tcgtaaaaaa agaa
//
LOCUS       DACDHJ010000026        22761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDHJ010000026 DACDHJ010000000
VERSION     DACDHJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.026"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..167
                     /locus_tag="IV223_000785"
     CDS             <1..167
                     /locus_tag="IV223_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8711091.1"
                     /translation="LTIILTIPTIVGSIYGMNIKLPIAERDDAFIWLVLLTLFICIVT
                     TYYLKKGKFL"
     gene            240..356
                     /locus_tag="IV223_000786"
     CDS             240..356
                     /locus_tag="IV223_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8711092.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(411..2093)
                     /gene="recN"
                     /locus_tag="IV223_000787"
     CDS             complement(411..2093)
                     /gene="recN"
                     /locus_tag="IV223_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8711093.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2111..2560)
                     /locus_tag="IV223_000788"
     CDS             complement(2111..2560)
                     /locus_tag="IV223_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8711094.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2667..3485)
                     /locus_tag="IV223_000789"
     CDS             complement(2667..3485)
                     /locus_tag="IV223_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8711095.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3494..4384)
                     /locus_tag="IV223_000790"
     CDS             complement(3494..4384)
                     /locus_tag="IV223_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8711096.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4386..4610)
                     /locus_tag="IV223_000791"
     CDS             complement(4386..4610)
                     /locus_tag="IV223_000791"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8711097.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4615..5952)
                     /locus_tag="IV223_000792"
     CDS             complement(4615..5952)
                     /locus_tag="IV223_000792"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8711098.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5953..6807)
                     /locus_tag="IV223_000793"
     CDS             complement(5953..6807)
                     /locus_tag="IV223_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8711099.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6904..7353)
                     /gene="nusB"
                     /locus_tag="IV223_000794"
     CDS             complement(6904..7353)
                     /gene="nusB"
                     /locus_tag="IV223_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8711100.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7346..7771)
                     /locus_tag="IV223_000795"
     CDS             complement(7346..7771)
                     /locus_tag="IV223_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8711101.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8017..9081)
                     /locus_tag="IV223_000796"
     CDS             complement(8017..9081)
                     /locus_tag="IV223_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8711102.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9253..9498)
                     /locus_tag="IV223_000797"
     CDS             complement(9253..9498)
                     /locus_tag="IV223_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8711103.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9637..10011)
                     /locus_tag="IV223_000798"
     CDS             complement(9637..10011)
                     /locus_tag="IV223_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8711104.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10094..11008)
                     /locus_tag="IV223_000799"
     CDS             complement(10094..11008)
                     /locus_tag="IV223_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8711105.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11029..11832)
                     /locus_tag="IV223_000800"
     CDS             complement(11029..11832)
                     /locus_tag="IV223_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8711106.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11865..12854)
                     /gene="manX"
                     /locus_tag="IV223_000801"
     CDS             complement(11865..12854)
                     /gene="manX"
                     /locus_tag="IV223_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8711107.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(12957..13448)
                     /locus_tag="IV223_000802"
     CDS             complement(12957..13448)
                     /locus_tag="IV223_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8711108.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(13639..16473)
                     /locus_tag="IV223_000803"
     CDS             complement(13639..16473)
                     /locus_tag="IV223_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8711109.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(16880..17173)
                     /gene="rpmA"
                     /locus_tag="IV223_000804"
     CDS             complement(16880..17173)
                     /gene="rpmA"
                     /locus_tag="IV223_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8711110.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(17186..17530)
                     /locus_tag="IV223_000805"
     CDS             complement(17186..17530)
                     /locus_tag="IV223_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8711111.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(17557..17865)
                     /gene="rplU"
                     /locus_tag="IV223_000806"
     CDS             complement(17557..17865)
                     /gene="rplU"
                     /locus_tag="IV223_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8711112.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            18147..18788
                     /locus_tag="IV223_000807"
     CDS             18147..18788
                     /locus_tag="IV223_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8711113.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(18850..20481)
                     /locus_tag="IV223_000808"
     CDS             complement(18850..20481)
                     /locus_tag="IV223_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8711114.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(20604..20963)
                     /locus_tag="IV223_000809"
     CDS             complement(20604..20963)
                     /locus_tag="IV223_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8711115.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21122..21195)
                     /locus_tag="IV223_000810"
     tRNA            complement(21122..21195)
                     /locus_tag="IV223_000810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21159..21161),aa:Arg,seq:tct)
     gene            complement(21504..22187)
                     /locus_tag="IV223_000811"
     CDS             complement(21504..22187)
                     /locus_tag="IV223_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8711116.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(22315..22704)
                     /locus_tag="IV223_000812"
     CDS             complement(22315..22704)
                     /locus_tag="IV223_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAP8711117.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT         6535 a         5029 c         3798 g         7399 t
ORIGIN      
        1 ctttaacgat tattttaact ataccgacaa tcgtcggcag tatctatgga atgaacatta
       61 agttacctat tgctgaaagg gatgatgcct ttatatggct agttctatta acattattta
      121 tttgtatcgt aactacctat tatttaaaaa aagggaagtt tttataaaaa agccctcttg
      181 tggaaatgga aaaaagattg tacacttgaa tcaactaatt aatgacgagg ttgaaaacaa
      241 tgcgtgaaaa aaaacaaaca agtacttttg ctaaagtaac aaaatttgtc atttggacaa
      301 tgctgatttt aacgattgga tctgttgtat tgactgctgt aatgagtgtc atgtaaaaat
      361 aaaatatagc tatcagctgc tgaaaaggac ctgaaccact gcgaggtggc tcaggtcctt
      421 tttgcgagtt tcaatagttc ttttgcatgt tcgagtgtga gttttgtaat ctcacttcca
      481 gccagcatac gtgcaatttc ttcttctctt tcttttgttt tcaactctga aacagaagtt
      541 tgcgtacgtc catcaatcac tgctttcaca atataatatt gatagtcggc gactgcagca
      601 acttgaggca gatgagtgat gcacaaaact tgagagtatt tcgatatttg agaaatttta
      661 tctgcgatgg cctgtgcaac tcttccgctc acacctgtat ccacttcatc aaaaataata
      721 cttgtcacac cttgctcaga agaaaaaata gttttcaaag caagcaacat ccgagacagt
      781 tcgccaccag aagcgacttt caccaaaggc ttcagtggct ctcctggatt ggtcgtaatg
      841 taaaattcga tttggtcaaa tccatcatga ttcaattgtc tattttcttc ttttaagaaa
      901 cgtacctcaa actcagtgtt ttccatatac agacttttca attcatgcag aatggatttt
      961 tccaattctt ttgcgatttt tttacgcgct tcatgaagat ctgcggcttg ttgctgaagt
     1021 agaagctctt tttgctctag ttcctctttc attttgtcca attgaccttc tgaaaaatca
     1081 gaagaagcta gctcttcggt gatttcgtca tagtaagcca atattgactc aatggattcg
     1141 ccgtatttac gtttcaactg tcggattaat tctaaacgtt gcgtaacttc ttctaaacga
     1201 ctctcatcta actccaataa atcaatctga cgactcatat cgccgattgc atcttgcagc
     1261 aaatagtaag cactttggat attttcataa attgcttcat actccacatc tagatgggcg
     1321 atcccttgca gttctgaaac ggccaagcca acaccatcta cactgttttg ttcattattc
     1381 gtcagtgctc cgtaacctgc agctaatcca tcagcaattt tttgataatt gctaagtttt
     1441 tctctttctt cttttaattt ctcttcttct ccgacttgca aatcagcagc agctatttct
     1501 tcttgctgga aatggagcat atctattctc tgaacatagg atttttcatt ttgctgcact
     1561 tttcgtaccc ttttttccag ctcacgataa gagatatatt cttgctgata actctttttc
     1621 ttctgttgga aagctacatc tccaaaacga tccaataaag caagatgtga ttcaggctgg
     1681 agcagttcct gatgttcgtt ttgtccttgg atatctacta aataactgcc gattcttctc
     1741 agattagcta atgtgataat atgaccattc actcgacaaa cattttttcc agttaaggac
     1801 atatcccgtc gaacaatcag attgtcctca tctgtttcga tccctaattc aaccatcagt
     1861 tcagagaatc cttcttgttt tggtagttca aacaaccctt caagcacaca tttttctgct
     1921 ccttgacgta tataatcgct tgaacctctt cctcctgcta acagacctaa agcatcgata
     1981 ataatggatt tacccgcacc agtctctccg gtcagcgcgg tcattccttc gtgaaaagaa
     2041 agccgcaatt ccggaataat cgcaaagtta gtaatactga tttcttgtag catcacattc
     2101 gctcctcaac ttaaaggtaa tgcaggaagt ctttcttcaa taaaactgcc tggtcttcag
     2161 ttcgtgtaat catcaaaaca ttatcgtcat cattgataat cgaaaataat tctttcttgt
     2221 aacgtttatc aatcaaattt gctgctgccg aagcatttcc cggcaatgtt ttcaaaataa
     2281 caaatttttc catttggtca actgccacga aagcgtcttt taatagtttt tccaatttag
     2341 tatttacatc ttcgtttgtt tcagctggta tgctataacg gtagccgcca tctgaagccg
     2401 gtaccttgat cagtttcagt tcctttatat ctctggaaat cgtagcttga gtaacagata
     2461 ttcctttgct tttcaggatt tcaacaaatt cttcctgctt tcggatatct tgctcactca
     2521 acaatcgagt aatcaaacga tgccgatctt tttttctcat tacgtcttcg ctccttagca
     2581 tattcttctt aatcaatgat agcttatttc cttggtataa taaagaagaa tccgcatact
     2641 tctcacttga ttttcacagt tacaatttat ttatggtcaa actgttcgtg tgcatttgaa
     2701 accacttctg ccatattaat agctgaattg attgttccgc tttcgggtac tttcctcaag
     2761 tgagccaaga actcgatatt tccttctccc cccgtgatcg gagaataact taattccagc
     2821 acatcatatc caccttgtgc cgcaaatgac aaaatgtctt ctaaaacttt ttgatgggtc
     2881 tgagggtcac gaacaattcc gtttttccct acagcttctc tgcctgcttc gaattggggt
     2941 ttgattaaag caatcacttc tccctcttct tttaaaatag catgtaatgg aggcagcatc
     3001 agttttagtg aaataaaaga aacatcgata acagcaactt ctggaattcc taaggtaaag
     3061 tcttcgggtt ttgcataacg aaaattcact ctttccatta cgacgactct aggatcttgt
     3121 cttattttcc aagctagctg attgtagcca acgtctaaag cataactcat ttttgcgcca
     3181 ttttgcaaag ctgcatcagt aaatccgcca gtggatgcac caatatctaa caatgttttt
     3241 ccttcaacag atagatcaaa tgctttgagt gctttttcta attttaatcc ccctcgagaa
     3301 acataaggaa gttttttacc tttgactctt aattcactac tcactggaat tttttctccc
     3361 ggtttatcaa accgctcgtt tttttcgttg taaatcaaac cagccatcac tgaacgtttg
     3421 gcttgttctc ttgtttcaaa taatccttgt ttgaccgcta atacatctac gcgttctttt
     3481 tccattcttt ccctcatttc tccacatcaa attgcttgat catctggcgc atcagcccgc
     3541 catcaaaaga aaaaacgtcc tcttctaatt tgtctatgat cttgttggcg gataacagct
     3601 caatctccaa cgcctcacgt gtcttttcta ggcctaataa acgcgggtaa gtattttttt
     3661 ccatacgttc atctttacca gctgttttcc ctaatgtttt cgttgtacca atcacatcga
     3721 gtagatcatc tcttatctga aaagctaaac ctaaatgttc ggctaatcgt tgaagtagca
     3781 ataagatttc ttcaggttgc ttcgctaata tgccaccagc cagcaaggca taacgaatca
     3841 aatgccctgt ctttctttca tgaataaatg ctaattcttc taaagaaagc tttttcgatt
     3901 cgccctcgat atctgcagct tgcccagcaa ccataccttg tgatcccgca caaacagcta
     3961 attgctgaag cagcagaagc ttaggagaat tacctaagtg tgccatactg attagctgga
     4021 atgctcctgt aagcaaacca tctccggcca atatagctaa tgcttcgccg aatactttat
     4081 gattggtagg ttttcctcta cgaagatcat cattgtccat cgcaggcaaa tcatcgtgga
     4141 tcaaggaata cgtatgcacc atttctaaag cagctgctac ttgatagacg ggaacatcaa
     4201 taggctcatc aaaggaagca acggccgtta aaagtaataa gggacgtatt cttttcccgc
     4261 ctgctttcac tgaataactc atcgcatcat agagtcctct atccgttgta tgctcatcta
     4321 aaaaagacaa gatttctttt tctacagcag gtagatgaat agctgaaaag tcagttaatc
     4381 tcatctcagc tttcctcgtt tccgtcaaaa tcaacttctt catttgattc tgtcatcatt
     4441 ttcgtcaagg tctcttctgc tttagctaat gtatcttggc aatgtttgct taattccatt
     4501 cctcgtttaa acgaatctag agctgattct aatggaacgt ctccttgttc taactgcatg
     4561 acaatttttt ctaattcttg cagtgactct tcaaaagttg gattttccat atttttattc
     4621 tcctttgtct atctcttgta cttgtccttt aacgattcca tcttgataat ggatcgtcag
     4681 caaatcccct tgatgaatct cggtgacact ttttactact tgatcattta acgtcgtata
     4741 gctgtagcct cttcccatga ttttcaatgg actcaacaaa tcaagagatt gaacagcttg
     4801 agcaaaacgc tgctgctgtt gtttcatata ttgttggatt ttctcttcta agcgtttttc
     4861 tagataagca gtttcttggc gagctgcttt aactcgataa agcggagcca cttgttccaa
     4921 ttggtggctt aatgcgatcg tttgtttttc tttcgcatga taaatttgct gtgtagcttg
     4981 atataatcgc tgtttcaact ggtctaattt gatggtctga ccttcataca atctttcagg
     5041 ctgccgaaat acataggaac gtcgagcacg ctcgaaccgt tcttgtttgc gctggatctg
     5101 gcgtacaaac gcctgctgca atctagcttg ccgttcttga atgcgcatca actcttctga
     5161 caaaacagga acagctaatt ctgcagctgc tgttggagtg gcagcccgaa catctgctac
     5221 taaatcagcg atcgtggtat ctgtttcatg tcctacagat gaaatcaccg gcgtattagc
     5281 ttgataaatc gcccgagcaa cccgctcttc attaaacggc cataaatcct caatcgaacc
     5341 acccccacga ccgatgatca ttgtatcgaa attgcccatc tcttctactc tctggatatt
     5401 cctgacaaca ttgtccgctg cttggtttcc ttgtacaact gttgggaaca aaaccaattg
     5461 gacaattgga tatcttcttt tcaccgtcgt gatgatatcg cggataaccg ctccgcttgg
     5521 actggtaagg accgcgattc gctttgggaa acgtggcagc atttttttag gcccttgaaa
     5581 aaggccttcc ttcatcagct tttcttttcg ttcttccaat tcttgatata aagcacctat
     5641 tccatcaggc tccatgtgct ctacatagat ttgataagaa cctcccgctt catataatga
     5701 aatacgccca acgacaagga ctttcattcc ttcttttggc tggaatcgta atttttggaa
     5761 tgctcctttg aacataatag cagatatttt tgcatggtca tcttttaagc tgaaatattg
     5821 atgggcattg gcacgtaaac ggaaattcga tatttcgcct gttaaatata cgcgttctaa
     5881 ataaggatcg gcatcaaatt ttcttttcaa atacttcgtt aatgtagtga cagttaaata
     5941 ttcttgtgcc atctattcac ctgctccaaa agcttcggca gattttacgg tttgatacat
     6001 cagcatcgtg atagtcattg gccctacacc ctttggcaca ggagtgatcc agcctgcaat
     6061 aggctccact tcatcaaatt tcacatcacc gatcagctta ccgttagcat cacgattcat
     6121 ccctacatct atcactacag cacctggttt tacgaattct tttgtgacaa aatgtccacg
     6181 tcctattgct acgactaaaa tatctgcttc ttgtgccagt tcagccagat tttcggtttt
     6241 agaatgagca acagtaacag ttgcatctgc catcaagagt agctgagcca tcggtttacc
     6301 gacgatattg cttcgtccga taactactgc tcgtttaccc gccggatcga tagaataagc
     6361 ttcaaacatt ttcatgatgc cgtatggagt acaaggaatc ttgtccggtt tacccgcaag
     6421 gagtctgcct aaattcatcg gatgaaaacc gtctacgtct ttttcagggc ggatagctaa
     6481 caggatcttt tcttcatcta tatgctttgg caatggtaat tggaccaata taccatgaaa
     6541 actaggatct tgattgtatt tttcaatttc tgcaagtagt tcgtcttcag aaatcgtctc
     6601 aggataacgc tctaccaatg aatggattcc gatttctttt gctacacgtt ctttatttct
     6661 tacatagatt tggctggctg gattttcccc taccaataaa acgactaatc cgggatggat
     6721 accattatct ttcaattttt ggattttttc agcaatctct gcctgcattt tttcagctaa
     6781 ttcttttccg ttaatcaatt ctgccatcta accactccca gatatttttt gtgttttcat
     6841 tctaccatag aattttcccg atttataaca ggtttatgat gattcataga aaaaagctag
     6901 gacttacgcc ccagcttcta attctttcaa tacattggat aaaacaccgt tgacgaattt
     6961 acgtgaacgg tcatcactaa atgtttttgc caactcgatt gcttcattta aagcaacagc
     7021 cgctggtaca tctgtcacat aagtcatttc aaaaatcgcg atacgcaaga tcaccaaatc
     7081 catttttgag atccgattga tgtgccaatt gttttttaaa tgtttttcaa tcactttatc
     7141 caattcatct ttttttgcac agacaccgcc aactagcgta tcaagataaa caggaacaaa
     7201 ctctgattca tcttcgttga tcatgtcgcg gtggtctaat tcgattgcgt tgaaaatcgc
     7261 atcttctttt gttaggtctg cattgaagtc taatggaaat aaagcctgca atgccatttc
     7321 ccgaatctcg tgtcttgata attctttatt cattctcttc ctcttcgtct tcaaaaagtt
     7381 cttcgattgt tggttgttct aatttttcag gaacaacagc tactacatgg atattgactt
     7441 cagataaatc gatatccgtc atgaaaagga cttgctggct gacccgttcc tgcatttcca
     7501 ttgctacttt aggaactgaa acaccatatt ctagataaca gtaaagatct actttcaagc
     7561 catcttcctc gtttgctaag taaacacctt ttccgtgagc tgcacgtcct aataattcag
     7621 tgacattgct ggcaaaagtt ccgcgcattc cgtatacgcc ttctacttta gaagctgcaa
     7681 tcccgataat tacttcgatg acttcaggag cgatcacgat ctctcctaaa tcttggtttg
     7741 cgtttaaaat caaatttttt tcatctgcca tttatttatt cctccctttg cctaagcacg
     7801 agatacatcc gaattctcta tgatgcggca tagtgatcac gtggtaagaa aactgccgcc
     7861 gatcagctgc aaactgctat gaattggaga aaacacccaa ctttattatc tcttacagct
     7921 taaaagttct tctatagtgt atcattaaat cgagtgattt tctataacaa agtctgtgaa
     7981 cactgatttt tacgcttact tcttctattg atgaaactat aagatgatca attcttttgg
     8041 tgaacgagtc aacacacgat ttccatcttc tgtgatcaac aagtcatcct caatacgtac
     8101 tccaccgatt cctggtaagt agatccctgg ttcatcagtg atcacatttc ctggtacaaa
     8161 ttgcttttct gcacgagaag aaacgtttgg tccttcgtgg atctctaaac cgatcccatg
     8221 tcctgtagaa tgtccaaatg cttccccgta accatgtttt gaaatataat ctctagcaac
     8281 tgcatctaat tggacaccag tcattcctgg ttttgcagca tcaataactg ctaattgagc
     8341 atctaagact atttgataaa tttcttttaa tttttcccca ggatcaccaa tagcgaatgt
     8401 acgtgtcata tcggaaacgt acccttcgta atagcaacca aagtccaatg tgatcaagtc
     8461 accttgttcg atgatttttt tgcttgctac accatgcggc atagccgaac gcaatccgct
     8521 agcaacgatc gtatcaaaag aaacacctga agcgccaaga gaacgcatat aaaagtctag
     8581 ttgatttgcg acttcaatct cagtcatgcc cggttggatc attttcagga tgtgatcata
     8641 tgccatatcc gcaatatgac atgctttttc gataatagcg atttcttctt catctttcac
     8701 ttctctgagt tcttcgatca tctgtgaaac aggtactaat tccgcatcga tgatttcttc
     8761 taaaaccgaa tattctaaaa atgagactgt tgtttcctca aaagctaatg agtgcagttc
     8821 ttctttgcgt actaagtttg ctacctcatc aaaaataggt ccgacatttt taataatttc
     8881 aaacccttga gcctgcgctg ccgcctgctc ggtataacga aaatcagtaa taaaaaatgc
     8941 tttttccaaa gtaataacag ctaatcctgt cgtacctgta aagttcgtta agtaacgaag
     9001 attgtacgga ctggtaacca aaaatgaatc caaattttct tcttgcattt ttttgcgcaa
     9061 tttttctaca cgtagcatca taatgaaact ccctctttct ttgcacgctt ttttccatta
     9121 tagcaaactt ttcttcgtac acgacagtta aataaaaatt tccggaaaac ttctttttat
     9181 tttaaataaa taaagccaaa aaggagtcac agtctcgctg caactccttt tagtttacta
     9241 ttttgtaaaa acttatgatc gttttccaaa gaaaaatgag acaactgcta ccaaaatcac
     9301 tgcgcctaaa atagatggaa taagtgccat acctgcagct tttggtcccc attcgccgaa
     9361 aatcgcttgt ccaatacttg aaccgactag tccagcaatg atattcgtta cacagcccat
     9421 cgaatttcct ttactggtga tcgcacctgc aattgctccg atcacggctc caacaatcaa
     9481 tgtccataac caactcataa tataacctcc ttctttgcta atcaatttta gcatatggga
     9541 tttaaagggg acaaataatt ggttttgcta tttcatattt tcttctgcaa agtaaaaaaa
     9601 taaagaaaac ctcctcttag aagttttctt tattttttac ttgcgtttaa acatactacg
     9661 aataccacgt ttcactacat caaagaaact caatgattgt accattctat cttcatctac
     9721 atattctctg atcgcacgaa gcactttttt aggttctttg gaagaaaatg tgtatgtacc
     9781 attttttttc gtttgaatgg catatcgagg aatccatttt ccctttagca tgactgatgc
     9841 tagtacataa tccacttctt cccatggaat ttggatatat ttgcgtctat ctcgcgcatg
     9901 gtaaaactca aaaccattat cacctatcat gatttgcccg taatctgtca gacccgtaaa
     9961 cgaagtagca tcaattacca agtctacttt tgtattgatt gattgaacca ttttctccct
    10021 ccagatattt gtttatgaac agtatactat ataaaaaaag ctcgggacaa cgcccgagct
    10081 atatttttat ggattataaa agtccgatga cgtgtccaac aacaccgact acgaataaac
    10141 caaggatgat aacgattgga gaaacctttt tcttaagcaa ccacatacat aacaatgtta
    10201 atcctaatgc agctaaacca ggaatcaatt ggtctaagtt gtcttgtaat gttgttacct
    10261 tttcaggaga aagggctaag ccattattaa cttgttcaaa tgcactcttg attccttcac
    10321 cacctgaagg caatttatcc cattcgatgt aagcaccttt atctaatttt acagttgata
    10381 caactggtga gaatttgatt gatacccatc tttgtacaag ggcagctaat acgaacatcc
    10441 ctaaaattga agcacctttt gttacgtctt gtaacaatcc gccagaaagg tcatcagtaa
    10501 ttttagaacc agctttgtag ccaaattctt gtgtatacca catgaatgcc caacgaataa
    10561 tgttccaacc taagaagaag atgattggtc caaggatatt tcctcccata gctaatgaag
    10621 cacctagtgc acctagcatt ggacgtactg taaaccagaa cactgggtca ccaacaccag
    10681 ctaaaggacc catcatacct actttaaccc cttgaatcgc tacatcatca acaggcgcgc
    10741 cattcgcacg ttcttcttcc agagccaatg taacccctaa aattggagaa gcaatgtatg
    10801 ggtgtgtatt aaagaattcc aagtgacgtt ttaatgctgc agaacgttct tcttttgttt
    10861 tgtataattt tttgattgct ggaatcatag aaaatgccca gccaccgttt tgcatacgtt
    10921 cgtagttcca tgaaccttga atgaaggtag agcgccacgc aacagctaaa cgatcttttt
    10981 ttgataattt gattttttct tctgccattt tattttcctc cttcaagatt aatagtcatt
    11041 caaaatgtcg cctaaagggt caccagagtt gcttccgcct ccattatttg atgaaccacc
    11101 cattttagaa aggttcaagt aaataagagc taatgcaaca cctaaagcac ctagagcaat
    11161 caatgttaat tgagaaattg cagctacaac gaaaccaata acgaagaatg gccatacttc
    11221 ttttgtagcc atcatgttga ttaccaaagc gtaacctaca gctacgacca ttcccccacc
    11281 gatagccata ccatctgtta accatgctgg cattgattca aggaatgatt gcacagtttg
    11341 tgctgggata aatagcaacg ctgctgctgg gaccgcgata cgaacccctt gtaaacatac
    11401 agcaatgatg tgccacattt caaccttacg gatgtttcct tcttcagcag ccgcatccat
    11461 caaatgaacg attggaacag cgattgtacg tacgatcatt gttaagaaca accctgcaac
    11521 agccaaagga acggcaatcg cgatcgcaga aggaacacct ttaacacctt gtccacctaa
    11581 aactaaaata attgctgatg ctactgatgc taatgcagca tctggtgcta cagcggcacc
    11641 gatatttgcc caacctaaag cgatcatctg aagcgtaccg ccaagtacga taccagcttc
    11701 gaggttaccg gtaactaaac cgatcaatgt acatgccact agtggttgat gaaattgaaa
    11761 ttcatcaaga ataccttcca taccagctag aaaggcaact aatacgacta aaattattga
    11821 aataatagac atgataagcc tcctataaat tttaatatta tttattacgc ttttgctaat
    11881 tcagcttttg cttttttcaa aatatcatcc atgttatcgc gtgaatcatt aggtacttta
    11941 cgtacatcga atttcacacc tttttgttca agcttctcaa acgcttcaac gtcttcttgt
    12001 cccatagaca atactttact tacaacgact ttaccgaccg aatgcgccat tgacccaaca
    12061 tttaattctt tgatgtctac gccaccttca acagctgtca atacgtcttc aggattttca
    12121 aataaaagca atgctttggt atttccaaaa cgagggtctt ttgctacttc gatcattttg
    12181 ctaataggaa taacatttgc ttttacacct ggaggagcag cttgttcgat tagtctctta
    12241 cgcagatcat cctttgatac tgcatctgaa acaacgataa tacggtttgg ttgcactgct
    12301 tttgtccaag ctgtcgcaac ttgtccatgt aacagacgtg agtcaacacg tgctaataca
    12361 tatttgattt ttccgtctcc gaccacagtt ccttctggaa gtgctccttt aggttgtgcg
    12421 tcttcaactg ctgctttcgg tgcttctgcc ggttctaatt cttccggttt tacttttaca
    12481 ccgtcttttg ctgtttcaat gatgtgtgct gcaatttcat gagcagattc cattgaaaag
    12541 cgtgatgcgt aggcttcaat caacattggt aaattcaaac cgctgacgat tgcccattta
    12601 tctttgtgct cttcgaataa tgtgtttgat tgattgaacg gtgttccgcc ccataaatca
    12661 actaagaata acacttcgtc ttgattatca aaagaagcaa ccgcttcttc caatttggct
    12721 ctcaagtcat ctggaccttc acttggtttc aaaatcacgg ctttgacgtt ttcttgttcg
    12781 ccaaagatca ttgaaccgga ttgtaagatt ccttcagcaa attgtccatg acttgctagg
    12841 ataatcccta ccatatttat acctcctatt tacttttgtt gtaacaaatt caaaaatatt
    12901 aataattgct aaccattaca acagaattat ttgacctttt ccaacaacca ttcttgctac
    12961 acctttttga ttttcgctag tagttccatc aaaaggattt tcctgtcagc aggtactttt
    13021 cgaatctcta actccacttc ttgactgttc aagtaacgaa aggaatcaat atcttgttga
    13081 tttactgaaa caaaattggt aatcattgat ttgccttcag taaaactcat accgccgata
    13141 ttcaacgact tcaactgtac accgcagcgg acaaccgctt caacatcttg gggattggtg
    13201 aataaaagca tcacttttgc tcgtgataat aatccactat gatagatttg aatcattttc
    13261 tgcacggtta ttacatttgc tgtgatcccc ggcggtgcag cttcttttag tagaaacttt
    13321 ctcaatttat cttgagcgac tacatcgctg atcacaatga tacgttcaac acctgtttgc
    13381 ttcgaccaaa ctgttgcgac ttgcccgtgg attaaacgat catctatacg tgccaatcga
    13441 atatccatct gcaaaactcc ttcttcctca ctacttcaca gaaaccccta tcatttctgt
    13501 gtgaacaacc acctcctagc acacactact ataagcaaaa agcgtgccaa cttttttacg
    13561 atacactaat tgaatttaaa ggattcttaa ccaatacaca aaaacgatac actataaaag
    13621 tgtatcgttt ttgtgtattc atggcacagt gtattctttt ttatctaact gattatggac
    13681 tagacgtagc agataataca tttcttctat cggcactttt acttttatct gatcttccaa
    13741 cagaacaact gaggctgcca attggctata aagaggatca ttcgttgtat ttgtcaattc
    13801 gttttcttcc acagtcaatg gttcattccg aataatacgt tcgatcattc ctgctgtgtg
    13861 catcactagg ttgattttga atccatattc ttcatcgccg attcccgtcg tctgagagat
    13921 ttgtgtagaa aattgccaca ttggatcgat taacttacta ccattaataa agatgaagtt
    13981 atcagagata aactcaacac acgtattttt agctgactct tcatctaggg agatgaattc
    14041 agtttcctcc aattctgatt ccatcagcaa ctgatccaaa atgacttcga tattttgatc
    14101 aataaaacgt tctaaaggaa taaatggggc ttggatattc aaatcagtaa tcccagtagt
    14161 agctatgatc tgatattctt tttggataac tggcagttct tctttcactt ccacaatcga
    14221 caaagctaga acttctaatt ctgtttcttg acgcttgttt accgctcgtt cgatcaattc
    14281 tttgattctt tgtgctgtac cttcgccaga tgcgcagata gccaaaacag cttttttctt
    14341 atgaataggt tttgcttcct gaacagattc tacagaagat atttctgcgt aacctcgaaa
    14401 atttttcaat gattcatgca acatatctag atctgtccct aatactgaga cctttcgtac
    14461 agtttccaaa actagagaag ttgttaccat atcaacagta cgtactggaa ttccagtgtc
    14521 tctttgaatt tgcgaattaa acgacgcaag ggaccccata tctactaaga gtatgacccc
    14581 gcttccttca tctacttctt gtacattgcg ttcaattcgg tccaatgctg ttttcggatc
    14641 catatccaaa ggcatgtcta ctgctcgtac gttctctgca tctaacaact gttggacaac
    14701 ttggaccata ctgcttgctg tgctgtttcc gtgagctgca acgactacac cgattctgcc
    14761 gcttgcttga tttgcccgta acgagacgag tagaacggtc aagtaatagt cttcactttc
    14821 tggaatctcc acttgaaaat acgtttcgat ataactacgc acttctttcg caacagcata
    14881 ttcatctgga aaatcaaaag ccatctcacg gatgttatca ttcgtatgcc gttcttctcc
    14941 taagttgatt tttttcaaaa aagaactgat atgcaagctc atagcataaa taaagttttg
    15001 ctgaaactca tatggtaaag atcttttgac cattacataa atctgattgg tcacttctat
    15061 gattttcgga tctacaaatt ctgctaattt gctgtctgca ttaaaagaaa aaccatggtc
    15121 tttatagaaa gacttcagat ggatgttgat atctgtagaa atgaaatgat tgattgcttc
    15181 ttgatccaaa ccatccgact tcagtaaagc tgctttatct ccgatgatat catatagatt
    15241 ataaggtaac tcataggagt ccgattggat tttcatgatc gtatcatttg gtgctacagt
    15301 tatctttggc tccaatattt tagaaagctc tgccattgca ctgcggttgc ttgctaattg
    15361 aatcaatccg ctacgaatcc cttctgtcaa atcatcgatt gtgatcgaga tttcgggtga
    15421 ttgcatatga tttagaaatc ctcgtgcaca tactaactgg atatttgact tcaattgtcc
    15481 tatatttcca taggtcacac tgccgatcaa tgcttttgct acatcttctg tcaaagaaat
    15541 ctttcgctgc gtgcggtttg cttcgtgggc aatcatgatt tttactaaat cgactttctc
    15601 atttgcagga cgtttttcaa aagccggaag tttgatattg attgggatac gccgaacaaa
    15661 agtttctaat agagaagaac ctgggtcttc tgtggtcgca cctacgatcc gtacatttgc
    15721 ttcatgggat ttcgttgttt ccccaagacg actgtaagtt ccatgatcca taaagtaaaa
    15781 gatcatttct tgaccttcag gcggcaaccg gtgaatctca tccaaaaaca gcataccgcc
    15841 atccgcttga tcgataatcc ccattttttc ttcatctgcg cctgtaaaag caccttttgc
    15901 gtacccaaat aaatgactca tcaaaagctc agggttattg gcataatcag cacaattgaa
    15961 aacaatcagt tccgtttcct catctattac atgatgcgct tttgcaaaat gatacatcgc
    16021 gtgtgcaaaa taagtctttc ctgatccggt aggtcctgta atcaaacaat ttaatcctct
    16081 tggcggatac aaaatcgcag cttttgcctg ttccactgaa tttttcatgc tgccgttagc
    16141 tccaatgatt tttgcaaaga tatccttagt atctatttgg agtgtatttt tttcttcaga
    16201 gaccggcgtt tctttgtaac tgactacatg ttttgtaact ggctttacgt gcgtaacgag
    16261 tgtttgatat atgtaacgta ctggtcgtcc gtctgtcttt atcagttttc cttcgcgcac
    16321 tagctgattc agatctttac tgctatttgt tcgctgaatc cctaacactt cagcaatttc
    16381 ctgtgttgtt actccttgat catattctgc ctttgttaaa tgagcggttt tttctttgac
    16441 atacatataa attcggtcaa tgcgttttgc caatggtatc gctccttttc tatagactat
    16501 tgtacttagg cttcaaaaca taatactatg tatttatttc tgtttcgtca actatttacc
    16561 tgattttgtt ttgttgataa cacgcggata gatagaaatg ttcaatttaa aagaacgaaa
    16621 aatcccgata cgatttttat aatggaaaca agaaaaagaa cactgtttac catataatta
    16681 tttcactgat agcaaacaat atagcttcca tcaattgaaa acgatagtta tcaaaacatc
    16741 ctgattccaa attggcaaaa aaatattaag tgtaaaaaga caaagtaaac ttcgtagcct
    16801 ccactacaac ttttattttt ataaacaaaa aaggctgtga caaaagtctt gtcacaacct
    16861 ctttaatcat aagattcaat taagcttctt gagctactgg ataaacagac acttgttttt
    16921 tgttacggcc tttacgttcg aaacgtacta cgccatcaac ttttgcaaat aaagtgtcgt
    16981 ctccaccgat acccacgttt acacctggat agatttttgt accgcgttga cggtaaagaa
    17041 ttgatccacc tgtaactgtt tgtccatcag cacgtttagc acctagacgt tttgattctg
    17101 agtcacggcc gttagatgta gaaccgccac cttttttgtg ggcaaataat tgtagattca
    17161 tagataacaa catggtctgc acctcctatt tatcattaat tgttttaatt tggatatatt
    17221 ctgagttttc agcttctatc gattgtaatc ctaataaaag attttctaat agaatctcag
    17281 caatattgtt ttgttcttgt gtcattcctg aagtgacttc ggtataaaga taaccgcctt
    17341 cttcttcatt catttcaaca atcggctcaa aaccggctaa tgaggcaatc ccgttgacag
    17401 tgctgatcgc taacgcagaa acacccgcac aaacgatatc acttccataa ggaccagcat
    17461 cagcatgccc ggtcaaagtg aatgaaacga tctgacctgc gtcatttcgc ttaaatgttc
    17521 ctttgatcat atataggcct tctttctcaa acgctattaa gcgttgatcg cgtcgataac
    17581 cacttttgta tatggttgac gatgaccttg tttacggtgt gaatgttttt taggtttgta
    17641 tttgaaagta acgactttct tttgtttgcc gtgtttttca acagtacctt caacagttgc
    17701 acctgcaaca gttggagcgc ctacttttgt agattctccg cctactaaga taacttcgtc
    17761 aaaaacaact ttttctcctg cttcaacgtc aagtttttca acatagattg cttgtcccac
    17821 ttcaactttt acttgtttac caccagtttt aataattgcg tacatgctat aaagcacctc
    17881 cttctgatat acttagactc gccatccgaa gtgacagcat atgctgtgct tattacttgt
    17941 tctgagcggt tgtagctgtg gagtccacaa ttacaacagt actagcttac cagaacagca
    18001 accgttagtc aactaaattt aaaatcaaaa agtaaaacaa aaattcaagc aatacttctg
    18061 cttttctatc ctcacagttc taccaaataa aatagtcgca ttgtgtttct tttattctta
    18121 taataaaaga aaaaggagga gcataaatgg aatacccaaa agaactgata caagaagctt
    18181 ctttaagaat gaacggccgt cctgtagaag gatttatcgc tgggcaagga cctcttcatc
    18241 ctaaactgat gctcgtggga gaagccccag ggaaaacaga gattgatacg catgttcctt
    18301 ttagcggtca agctggaaaa gaattgatgc aggcactttc ttcaactggc ttaacacgag
    18361 aagaggtgta tatcaccagt gctgtaagga gccgcccgta tcgcgtgact catcggatca
    18421 ataaacggac acaacagtca gaaactgttt atccaaatcg gacacctact cgttccgaag
    18481 tatttgcgca tgcaccaata ttggattacg aactgatgaa ggtccagcca aaattaattg
    18541 cgacacttgg aaacattggt cttcaacgtt tacttggcaa agaatatacg gttaccaaaa
    18601 atcatggtca actctataca gggcctgtct tacaattaac agaacaaaag gatgcctacg
    18661 aatggtcaga aaaaaactat cgaattcttc cactttttca tccagcagct attttctaca
    18721 atcgctccct tgtacctgtt atccatcaag actggcaagt actagcttca cttttacagc
    18781 aatattaaaa agaacagaca agaccataaa atcttcagtt tacagtcttg tctgttcttt
    18841 ttcattgttt taatggctaa ttgcagtata tttcgcatgt aaagctgtca acgcatgagc
    18901 aactgtttga tgttgatgcg tgaatgtatg ttcccacaag tgagaaagtt gtttttctac
    18961 ttctggctgc aaggaagtat agataatcgt ttgtcctctt tgctctgctt tttcttgaaa
    19021 ttcatcaaaa actgcacgtg ccgtcaaatc aatacttggt acgcctcgca tggagaaaat
    19081 gacaccatcg tgatgattta gttcttccaa tttatttttg agtttttctg aagacataaa
    19141 aaacagtgga ccactaatat acactactgt ccagttattc agcttttccg tttctggcag
    19201 ccccattctt tgccaatcga tcgtttctaa tgaaatcgtg atagcagcgc ttttgacaac
    19261 aaaaaataca cagccgccga tcaccccgat cacgatagca atacttaaat caaagataac
    19321 tgtacaaatc atcgtaagga taaacaatag aactgctgac caatatttat tagtaaatag
    19381 ctccttgatc gtttcccatt cattcatacg ccaagccgtc acaatcaaaa cacctgcaag
    19441 tgctggcatt ggaatctgtg acatcactgg cgcaaaaaca agcatggaga gcagtaagac
    19501 aactgcatgt atcataccag ctaatctagt ttgtgcacca gatttgatgg ccacgcttgt
    19561 acgggcgatg gccgcagtag caggaatccc gccaaagaaa ggaagcaaca gatttccaat
    19621 tccttgcgcc attaattctt gattgctatc taagggctta tttgccattc ggccagccga
    19681 agcaccacat agcaagcttt caatcattcc taataaagca atgctaatag ctggaactgc
    19741 cacttgttgc aaggcagaaa agctaaaatc tcccatattc aaacgattac tactgatcaa
    19801 tgtttgtgga atcttaccca cagttgctac tggtaaatgg aacaatagca taactgctgt
    19861 tgccaaaatg atagccagca gcgaactagg cattcgtttc gcccattttt ttggatagat
    19921 aaacatccct aaaatgacta gccctcccat gatcaaagag gctgtgttta tcggaaatcc
    19981 tatcgtttga taactactta gtttttcaat aacgtttgta ccttccgagt gaacaccaaa
    20041 taaattatcg atctgaccta atgcaataat gatagcaata cctgaagtaa accccgtaat
    20101 tacgggtgct gggataaaag aagtcagtgt tcctaatcgg aaaagtcctg ctgcaagtaa
    20161 aaatagaccc gctaaaaaag tagcaagcaa tacaccttgc attccctttg ttgccgcaat
    20221 agacatcaag atagctgcca tcgcacctgt cggacctgat atttgataaa aaccgcctga
    20281 taaaccgcca ataacgatac cggcaatgat agccgtaatc aaacctgcgg ccgcatcagc
    20341 tccgcttgat accccaaaag ccaaggctaa aggcaatgct acagcagcta ctgtgatccc
    20401 agccaaaaga tctttctgaa atcttgaaag atcataacct gaaaattctt tttttagtga
    20461 ggaaacataa tttttgaaca cctatctaac tcctttttca tgaaattttc taaaatacca
    20521 ttcattagaa tatcatattt catgagataa agtaatattt ttcacaaagt ttcctgttag
    20581 ataaattctt attttttcta tatttataag aaaatagaaa gcaatgccaa cagagctagc
    20641 ccaccttgtt tgagtaaaat ccaacgatcg ctagtcaaac tgccatataa tgcaacaaaa
    20701 aaaatagata ttaatagcat acttgtgatt tctttagatg cggaagaaag ataagctcca
    20761 taaagtagtc ccactcctaa tagtccattg tacactcctt ggttcttaaa caatgtttga
    20821 acagattttc tgcttaactc ttctttactc attttaaaaa ccctacttgt cgtgtcagaa
    20881 tcagttctca ccgtttcgat atacatgata taaaaaaatt caattgcaac tagagtcact
    20941 aaaattctag atagtatcgt catatcttct tcctcttttc tgatgagttt tatttttttg
    21001 ctccttataa ttgtactcga taaggatgtt tttttatttt atttgccttt gaaaacaaac
    21061 aatattggca caaaaaacaa agaaaaaccg ttagaaacct cagtttctaa cggtttttta
    21121 gcgtcacagg agggattcga acccccgacc gtccgcttag aaggcggatg ctctatccag
    21181 ctgagctact atgacaagaa tcagcggaac atcaataatt atagataatt aaattgcttc
    21241 tgtcaatacg aaatagttat ttaattagca aataatgaat tattttggcc caactactaa
    21301 atcaatcttt taaaacgaga cattctcctt cagctgttct aaggttatca attgaaaaca
    21361 ggagctacat tgacttcatg ctactcctgt ttatttagat ttcctgtatc ctttaattga
    21421 cttacaaata tagaaacttt tacatcaagt tcgaattgag ccctcgattc aatcgattat
    21481 ctacaggctg ccataatcat gtatcacaac tcgattgttt ctgattcctt tgtcattaag
    21541 atcacaccat cacctaatgg caagattgtc gatgtcaaat cgggatgatc catcacaaca
    21601 cgaagaaatt catttaattt acgatggata gctcgtttgc cacggggaat ctcttcatct
    21661 gggagcaaga tcgttcctcc ttggaagatg tcatctacca tcaaaacgcc accttttctt
    21721 aacaaacgta agcattcagg taaaaattca atatattttg attttgcact atccataaaa
    21781 ataaagtcat acggaccaga caactccggc aagatatctg ctgcttgacc ttcaagcaaa
    21841 gtcactttat ctgtcagtcc tagacgttca tagttttttt tagctttctc gatcatgaca
    21901 tcgaaacgat caattgtcgt aacatgagcg ttttctccga ttgtcgtggc catgacactt
    21961 gatgaaaaac cgatagctgc tccgatctcc aagattcgtt caggctgaag ctgtccaagt
    22021 aaaaatttca aaaatacaac tgtttcatgg ggaataactg gtacttctgc tacatgagct
    22081 tcttcttcca gtttgcctag ctcacccggt aatttttttt gctgggtacg cataaaatcc
    22141 aatagttctt ctttgactac aggacgatgc atcatttcat tgcgcatact actcctcctc
    22201 ttcacacatt atacgaacaa ctgactaaat ctacaagctg catgagtgtt ttctcgtgtt
    22261 tcatgttcag aagagtcgtt ggatctctgg aatacattta tgtttttaac attcttacaa
    22321 aaatttctgt gttcgtctcc gcttcttttt caactctaca aaggtattcc atgctgtgat
    22381 tcctgttagg caaaaagcaa tcgtaaagaa taacccctga atccatgcaa atgccgtttt
    22441 tgaaaggact agtccaatat atggaatctg aaagatggga ctaccgataa tatttctctt
    22501 ttttactact ccaggctcaa tataagtctt tgtatcagct tttgtataaa aatcttcgtg
    22561 gttgttttca acggctatta cgcgacgcgt cgagattttt tttcctgaat tttcataata
    22621 tgtaataata tctccgacta aaatctcatt tgtatttttc ggtcgtacat atatcagatt
    22681 tccttgtgca taatatggac tcatttcttt gtcctcaata atataaggag tcaaaccaaa
    22741 aattcgggga accaagacga c
//
LOCUS       DACDHJ010000027        22641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDHJ010000027 DACDHJ010000000
VERSION     DACDHJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.027"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..336
                     /locus_tag="IV223_000813"
     CDS             <1..336
                     /locus_tag="IV223_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8711118.1"
                     /translation="LPIPGSILGILFLFLAFEMHLVDPEKIGATGDFLLNNLAILFVP
                     AGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGKTAEGVEALLGYVRKKKAERVSV
                     NEMEENQID"
     gene            329..1030
                     /locus_tag="IV223_000814"
     CDS             329..1030
                     /locus_tag="IV223_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8711119.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1030..2616
                     /locus_tag="IV223_000815"
     CDS             1030..2616
                     /locus_tag="IV223_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8711120.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2635..2856
                     /locus_tag="IV223_000816"
     CDS             2635..2856
                     /locus_tag="IV223_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8711121.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2853..3614
                     /locus_tag="IV223_000817"
     CDS             2853..3614
                     /locus_tag="IV223_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8711122.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3994..4503
                     /locus_tag="IV223_000818"
     CDS             3994..4503
                     /locus_tag="IV223_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8711123.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4578..5117)
                     /locus_tag="IV223_000819"
     CDS             complement(4578..5117)
                     /locus_tag="IV223_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8711124.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5257..5868)
                     /gene="rpsD"
                     /locus_tag="IV223_000820"
     CDS             complement(5257..5868)
                     /gene="rpsD"
                     /locus_tag="IV223_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8711125.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6184..6981
                     /locus_tag="IV223_000821"
     CDS             6184..6981
                     /locus_tag="IV223_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8711126.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            7009..7644
                     /locus_tag="IV223_000822"
     CDS             7009..7644
                     /locus_tag="IV223_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8711127.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7664..8437
                     /locus_tag="IV223_000823"
     CDS             7664..8437
                     /locus_tag="IV223_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8711128.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8845..9642
                     /locus_tag="IV223_000824"
     CDS             8845..9642
                     /locus_tag="IV223_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8711129.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9620..10033
                     /locus_tag="IV223_000825"
     CDS             9620..10033
                     /locus_tag="IV223_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711130.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10017..12662
                     /gene="mutS"
                     /locus_tag="IV223_000826"
     CDS             10017..12662
                     /gene="mutS"
                     /locus_tag="IV223_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8711131.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12680..14788
                     /gene="mutL"
                     /locus_tag="IV223_000827"
     CDS             12680..14788
                     /gene="mutL"
                     /locus_tag="IV223_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8711132.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14810..15370
                     /locus_tag="IV223_000828"
     CDS             14810..15370
                     /locus_tag="IV223_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8711133.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15455..17443)
                     /gene="treP"
                     /locus_tag="IV223_000829"
     CDS             complement(15455..17443)
                     /gene="treP"
                     /locus_tag="IV223_000829"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8711134.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17666..18379
                     /gene="treR"
                     /locus_tag="IV223_000830"
     CDS             17666..18379
                     /gene="treR"
                     /locus_tag="IV223_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8711135.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18456..18902
                     /gene="msrB"
                     /locus_tag="IV223_000831"
     CDS             18456..18902
                     /gene="msrB"
                     /locus_tag="IV223_000831"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8711136.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19072..19674
                     /gene="ruvA"
                     /locus_tag="IV223_000832"
     CDS             19072..19674
                     /gene="ruvA"
                     /locus_tag="IV223_000832"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8711137.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19687..20688
                     /gene="ruvB"
                     /locus_tag="IV223_000833"
     CDS             19687..20688
                     /gene="ruvB"
                     /locus_tag="IV223_000833"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8711138.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20717..21295
                     /locus_tag="IV223_000834"
     CDS             20717..21295
                     /locus_tag="IV223_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8711139.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21347..21829
                     /locus_tag="IV223_000835"
     CDS             21347..21829
                     /locus_tag="IV223_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8711140.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21946..22320
                     /locus_tag="IV223_000836"
     CDS             21946..22320
                     /locus_tag="IV223_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711141.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7370 a         4059 c         4902 g         6310 t
ORIGIN      
        1 ttacctattc ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat
       61 cttgttgatc ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt
      121 ttatttgttc ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg
      181 cctgttttac tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa
      241 acggcagaag gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta
      301 tctgtaaacg aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg
      361 cttaagtttg actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact
      421 gcctgttttg aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc
      481 tgggatcagc tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc
      541 tgcgacagtg gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt
      601 ttatcctgta atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg
      661 gttcggttac ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt
      721 aacgacggct atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact
      781 tgctattgtt gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct
      841 attcaagatc gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat
      901 tggtacagca aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat
      961 atgcgtcaac ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg
     1021 cttgttttga tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa
     1081 aaaggaggga aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta
     1141 tattataaga ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa
     1201 ttagcgcaag ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct
     1261 aaaaactatc agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct
     1321 ggttataact tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc
     1381 gtgcaaagta cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca
     1441 atgtaccaat ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat
     1501 gaatggaaac agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct
     1561 caaggcgaag aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga
     1621 gggaaaatct ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat
     1681 atcatggctt tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg
     1741 ctaggtgcct tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa
     1801 agacggctga tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta
     1861 tttcctaatt attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact
     1921 cgtgaagaat attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa
     1981 gcaaaaacac cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag
     2041 gaagagcagg ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc
     2101 agtcattttt tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt
     2161 gattttatta atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt
     2221 tttgaaagta gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg
     2281 gggcggtatc tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc
     2341 acgatccttt taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta
     2401 cggacatttt tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga
     2461 gaagttgatc ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac
     2521 tattttattg tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt
     2581 gaaatgattc aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca
     2641 tttgaagaaa ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt
     2701 ggatttgaac tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg
     2761 ccgtacttat tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt
     2821 gatattttag cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta
     2881 aacaggttgt atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt
     2941 ttcttgaaca agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa
     3001 tttctttgcg atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag
     3061 aactccaaca agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga
     3121 atacagcggg gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg
     3181 atctgattta tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg
     3241 atatgatccg ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg
     3301 ctggtggagg tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac
     3361 aactcgctct ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca
     3421 tccaaacacc aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg
     3481 caaatgaaac accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat
     3541 ttcttgcctc tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat
     3601 ggttgttaaa ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa
     3661 acaacggttg atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca
     3721 gttcaaaaaa ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct
     3781 cttgatataa acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa
     3841 atataaggag ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac
     3901 taccttctgc gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca
     3961 agataaaatt atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc
     4021 aaacgctgga caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca
     4081 gtgacgctgg ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg
     4141 ttcaactatc tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct
     4201 ttggctaata ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg
     4261 ctgcttgtat tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa
     4321 atggccgtga cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg
     4381 attttatatc aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca
     4441 tcaatgattg tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa
     4501 taaagatctg tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt
     4561 ccatgctgct tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag
     4621 ataccgatat aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt
     4681 ccactggcaa atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg
     4741 cagatcaatg cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca
     4801 tccgttcttt cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat
     4861 ccgccagttg gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga
     4921 ccaatcaagc caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga
     4981 aaagttgctg ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga
     5041 atggtcatct gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct
     5101 ttcaatgttc ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg
     5161 aaattgtaaa acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc
     5221 aatagatagc ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc
     5281 aacgacaaga gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa
     5341 tgaaccttcc aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc
     5401 tacagcttct tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc
     5461 cacttctacg tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg
     5521 gttaactaat tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt
     5581 gtctaaacgt tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt
     5641 gctagctttt acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag
     5701 tttttgtttt tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc
     5761 atgttgacct ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat
     5821 gcctaagcga cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc
     5881 caatgaatat aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt
     5941 gttcttcaat cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca
     6001 aactagggat gagttattat ttttctgctg cattatttta cacaaattac agtatatgat
     6061 agatcatcca cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt
     6121 ctttataaaa tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa
     6181 tcaatgaatc cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg
     6241 aatctttttg aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt
     6301 ttgactttag gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat
     6361 ggtctaggaa agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac
     6421 atgaacgccg aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag
     6481 agacttgctt taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt
     6541 ggcggtatat tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg
     6601 gacaacttta tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg
     6661 gtggaaggga tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg
     6721 aaagaggatg tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta
     6781 ggatatgaac acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac
     6841 ggacagatgg atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca
     6901 ggaaattata ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca
     6961 aaatcatcct atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta
     7021 gtttttccaa aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt
     7081 tcctttgctg gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag
     7141 agcaaaaatt tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc
     7201 gaaagagagt atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca
     7261 tttgaagact tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc
     7321 gactcttgga gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa
     7381 gtctttcaat tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg
     7441 caattaggtt ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat
     7501 cgtcaaacaa aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt
     7561 aagaccgatg ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt
     7621 cgagaggcaa gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt
     7681 tgcttttgat attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag
     7741 agaagcattg gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag
     7801 ccgatttcat gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa
     7861 tggagcggcc ggttttttag accatgaaca agtatataag aatttactag atcgcgacca
     7921 gttggatgcg ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt
     7981 agacaatatc aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc
     8041 tttcggtgct gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca
     8101 agcgttagca tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg
     8161 atttgtccgc tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc
     8221 gacaattctg cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg
     8281 cgatggacaa aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa
     8341 tgctgtgcca gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg
     8401 aatttggaag gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc
     8461 tgcatcaagt aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg
     8521 gcttattacc gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt
     8581 ggtcagtttc aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat
     8641 tacggcttat ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac
     8701 aagatgtttg tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga
     8761 aagagttcct tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat
     8821 gttaaaagag aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca
     8881 atgggacgag agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa
     8941 gtaacgattg taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat
     9001 aaaaaatttt tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac
     9061 cgcggaatct ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg
     9121 gaagggacgc ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg
     9181 atcaacatgc aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat
     9241 gagctggtag aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa
     9301 acaacaagtg aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc
     9361 ggtacacata cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat
     9421 ctgacagacg ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct
     9481 gtaatcgaaa agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga
     9541 tcgattttaa gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc
     9601 cagctgattc agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt
     9661 gcagcaggag ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa
     9721 tgaaacgatc attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc
     9781 tgagttgaga gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga
     9841 caaaccagca gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga
     9901 tcagcatccg cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca
     9961 tcatttaaca actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc
    10021 ctcaaaaaac taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc
    10081 aagatgcttt tttgttctat cgtttaggag acttttatga actattttac gaagatgcga
    10141 tcaaagcgtc acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac
    10201 ctattcctat gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg
    10261 aaaaaggcta taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga
    10321 tggtcaagcg cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat
    10381 tgtcggcaaa agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct
    10441 ttgcttatgc cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag
    10501 ctgttttaaa tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca
    10561 ctgataccct tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg
    10621 agatggaaga aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa
    10681 aagaagcaac cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc
    10741 atatccaaaa agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa
    10801 aattcaacct agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat
    10861 ggttgttaga tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc
    10921 gtccgcttat ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag
    10981 acagctattt tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac
    11041 gcctagcagg tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa
    11101 cttctttaga acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat
    11161 ggcaaagtct gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag
    11221 ctatccaaga tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca
    11281 atgagcagct cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac
    11341 ttgaagcaaa agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg
    11401 ttttcggtta ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt
    11461 atgaacgaaa acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat
    11521 tggagacaca aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt
    11581 tagctgtgag agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa
    11641 tcagtgcagt agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc
    11701 ggccagaact tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag
    11761 tagaaaaagt attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg
    11821 aaatgatctt gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt
    11881 tggcattaac tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt
    11941 tacctatttt tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac
    12001 aaagtacttt tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa
    12061 acagtttgat tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt
    12121 tggcccaagc aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta
    12181 ctcattatca tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg
    12241 ttggagccgt tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg
    12301 cggataaaag ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag
    12361 aacgagcagc agatatcctg catacgctag aaaatggcga taacggacac catactgttc
    12421 cagctgaagt agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag
    12481 aaaaagtaaa tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt
    12541 tattcagtga attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt
    12601 tactagaaat gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt
    12661 aggaagaagg cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca
    12721 gatcgctgcg ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa
    12781 tgcaattgac gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac
    12841 gatccaagtg attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa
    12901 gcggcatgcg acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg
    12961 ctttcgaggg gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc
    13021 gaatcaggaa gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca
    13081 tcgaccagca ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac
    13141 gccagcccgc ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat
    13201 cgtcaatcga ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa
    13261 caaaatgctg gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg
    13321 attatctacc gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt
    13381 tggctatgtt tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat
    13441 caatggacgt tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc
    13501 aaaattaatg gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt
    13561 agtagatgta aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact
    13621 aacagagcta attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag
    13681 tgcagcggat aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat
    13741 cgaaatcgat ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa
    13801 ttttgatcga gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag
    13861 aattgttgat aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac
    13921 cacttatcca gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt
    13981 tgtagaaaaa acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga
    14041 gaaaacaaat gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga
    14101 agaattgaca ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca
    14161 agccatcaag aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt
    14221 tggacagatg cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga
    14281 tcagcatgct gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt
    14341 atctgatgat ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc
    14401 tttgaaatta aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt
    14461 tggtcaaaac agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga
    14521 atcgattatt cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa
    14581 attccgtgaa gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta
    14641 tttgaatgaa gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt
    14701 caattgtccc catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat
    14761 gttcaaacga atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt
    14821 attagcatcc caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga
    14881 gattgaacct gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt
    14941 tcgtagaatg gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca
    15001 tttagtgatt gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga
    15061 agatctggct gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata
    15121 tacagctgtt gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt
    15181 cactttttat cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc
    15241 ggataaggca ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc
    15301 tggagattat tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac
    15361 attcatttaa acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt
    15421 tttttttatt gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga
    15481 ctgcttcgcc cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat
    15541 tattggtcaa agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat
    15601 ccatttcagc aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga
    15661 aaggaacacc ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct
    15721 cattagaaag aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta
    15781 ctggtgcata gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt
    15841 gtgagaaaac tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga
    15901 ataatgtttc ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg
    15961 gcgttgctgt tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag
    16021 caggatcaat cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa
    16081 tgatgacagc aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta
    16141 gtattcctgg caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa
    16201 acatgccagc taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat
    16261 tgattccgaa catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg
    16321 agatttgttc ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc
    16381 cttgggcgat atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa
    16441 tcagctgcat atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat
    16501 acaggaatcc gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta
    16561 cgatatttga gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag
    16621 ctaaaacagt tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga
    16681 tatatttgcg gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa
    16741 taacttgcgc ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa
    16801 tatctgctgc ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg
    16861 taattcctaa aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag
    16921 tgatacctac tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga
    16981 caccattcca aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac
    17041 cttcaagtac atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg
    17101 gtgtaaagat ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg
    17161 cagctgcttt tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt
    17221 tataaaaagt agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca
    17281 tcccttttac gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca
    17341 ggacaaaacg catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga
    17401 tatcatgtaa cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc
    17461 tcttaacttg tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg
    17521 caagcgtttt attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag
    17581 cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga
    17641 tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga
    17701 acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt
    17761 gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac
    17821 ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt
    17881 cgactttcct atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc
    17941 aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat
    18001 cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg
    18061 cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc
    18121 agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc
    18181 ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga
    18241 tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg
    18301 ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc
    18361 ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt
    18421 ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa
    18481 aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa
    18541 cagaacgacc atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata
    18601 tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc
    18661 catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg
    18721 gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta
    18781 ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat
    18841 tcattccata tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat
    18901 aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt
    18961 tattcaagcg attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa
    19021 atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa
    19081 tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt
    19141 atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa
    19201 ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat
    19261 tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa
    19321 agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc
    19381 agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg
    19441 atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt
    19501 gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct
    19561 cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat
    19621 cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga
    19681 gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg
    19741 gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa
    19801 gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg
    19861 ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg
    19921 accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca
    19981 atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct
    20041 agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta
    20101 ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc
    20161 gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct
    20221 catatggaat attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt
    20281 tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg
    20341 cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac
    20401 gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct
    20461 ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga
    20521 ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat
    20581 atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt
    20641 gctactgctt atgcctatga acattttggt tacgactatc atatatgatt tttgctttta
    20701 gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt
    20761 gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga
    20821 agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg
    20881 ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa
    20941 ttgggaagag caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc
    21001 aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa
    21061 atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa
    21121 actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac
    21181 gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga
    21241 tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa
    21301 aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt
    21361 atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg
    21421 aatagcatta gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta
    21481 tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga
    21541 tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat
    21601 cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca
    21661 ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga
    21721 cccatactat accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca
    21781 ttggtggaat atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt
    21841 tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa
    21901 aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca
    21961 gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat
    22021 tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt
    22081 cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat
    22141 cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa
    22201 gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa
    22261 caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa
    22321 tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat
    22381 aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca
    22441 tgacagcatt cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa
    22501 aggtcctgct agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag
    22561 ggttgtttgt tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt
    22621 tttcgaaagt tgttaagttt t
//
LOCUS       DACDHJ010000028        22522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDHJ010000028 DACDHJ010000000
VERSION     DACDHJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.028"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..640
                     /locus_tag="IV223_000837"
     CDS             <1..640
                     /locus_tag="IV223_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HAP8711142.1"
                     /translation="LTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALR
                     VQLPDLQEMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIEL
                     VTYFIRRIQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFY
                     YEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(697..927)
                     /locus_tag="IV223_000838"
     CDS             complement(697..927)
                     /locus_tag="IV223_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8711143.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1013..1900)
                     /locus_tag="IV223_000839"
     CDS             complement(1013..1900)
                     /locus_tag="IV223_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8711144.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(1989..3212)
                     /locus_tag="IV223_000840"
     CDS             complement(1989..3212)
                     /locus_tag="IV223_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8711145.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3229..4308)
                     /locus_tag="IV223_000841"
     CDS             complement(3229..4308)
                     /locus_tag="IV223_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8711146.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4439..6166)
                     /gene="ptsP"
                     /locus_tag="IV223_000842"
     CDS             complement(4439..6166)
                     /gene="ptsP"
                     /locus_tag="IV223_000842"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8711147.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6166..6432)
                     /locus_tag="IV223_000843"
     CDS             complement(6166..6432)
                     /locus_tag="IV223_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8711148.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            6911..9145
                     /locus_tag="IV223_000844"
     CDS             6911..9145
                     /locus_tag="IV223_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8711149.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9293..9613
                     /locus_tag="IV223_000845"
     CDS             9293..9613
                     /locus_tag="IV223_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8711150.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(9818..10041)
                     /locus_tag="IV223_000846"
                     /pseudo
     CDS             complement(9818..10041)
                     /locus_tag="IV223_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(10239..11819)
                     /locus_tag="IV223_000847"
     CDS             complement(10239..11819)
                     /locus_tag="IV223_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8711151.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12220..13596)
                     /locus_tag="IV223_000848"
     CDS             complement(12220..13596)
                     /locus_tag="IV223_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8711152.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(13724..14842)
                     /locus_tag="IV223_000849"
     CDS             complement(13724..14842)
                     /locus_tag="IV223_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8711153.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            14984..15418
                     /locus_tag="IV223_000850"
     CDS             14984..15418
                     /locus_tag="IV223_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8711154.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15496..16029)
                     /locus_tag="IV223_000851"
     CDS             complement(15496..16029)
                     /locus_tag="IV223_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8711155.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16123..17301)
                     /gene="mutY"
                     /locus_tag="IV223_000852"
     CDS             complement(16123..17301)
                     /gene="mutY"
                     /locus_tag="IV223_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8711156.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17294..18091)
                     /gene="recX"
                     /locus_tag="IV223_000853"
     CDS             complement(17294..18091)
                     /gene="recX"
                     /locus_tag="IV223_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8711157.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18198..19580
                     /gene="rlmD"
                     /locus_tag="IV223_000854"
     CDS             18198..19580
                     /gene="rlmD"
                     /locus_tag="IV223_000854"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8711158.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(19754..20332)
                     /locus_tag="IV223_000855"
     CDS             complement(19754..20332)
                     /locus_tag="IV223_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8711159.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            20654..21124
                     /locus_tag="IV223_000856"
     CDS             20654..21124
                     /locus_tag="IV223_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8711160.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21232..22458)
                     /locus_tag="IV223_000857"
     CDS             complement(21232..22458)
                     /locus_tag="IV223_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8711161.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         6657 a         4574 c         3883 g         7408 t
ORIGIN      
        1 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
       61 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
      121 gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
      181 ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag
      241 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
      301 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc
      361 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
      421 ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta
      481 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
      541 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga
      601 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta
      661 ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc
      721 catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa
      781 ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc
      841 aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat
      901 gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt
      961 gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt
     1021 ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat
     1081 tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca
     1141 atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt
     1201 tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag
     1261 accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt
     1321 ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct
     1381 gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac
     1441 caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga
     1501 tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat
     1561 ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtatttgtag
     1621 tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt
     1681 agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg
     1741 ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg
     1801 cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag
     1861 cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat
     1921 caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg
     1981 tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct
     2041 tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc
     2101 atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga
     2161 tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt
     2221 tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc
     2281 acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa
     2341 gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc
     2401 acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac
     2461 tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg
     2521 acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat cctgcttaaa
     2581 gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc
     2641 tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga
     2701 agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact tggacaaact
     2761 acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc
     2821 acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc
     2881 agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta
     2941 tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct
     3001 ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca
     3061 tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca
     3121 tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga
     3181 aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac
     3241 ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt
     3301 tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg
     3361 tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt
     3421 tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc
     3481 tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc
     3541 ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc
     3601 acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat
     3661 gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat
     3721 cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca
     3781 aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat
     3841 tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg
     3901 gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc
     3961 tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat
     4021 aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc
     4081 tcttctggct ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat
     4141 gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt
     4201 ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata
     4261 taataatacc ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact
     4321 gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt tttcttaaaa
     4381 aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt
     4441 attttacata ttcatgaaca agttcaacga cttcttccat catatcgcat tctttcaatg
     4501 cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt
     4561 taagaataga tgtcgcactc attgagaact catctagacc catacctact agcaatggaa
     4621 cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg
     4681 ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt
     4741 aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac
     4801 ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa
     4861 tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca cttatcaatt
     4921 tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca
     4981 tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg
     5041 tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga
     5101 atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca
     5161 ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat
     5221 attggtcatc ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca
     5281 gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt
     5341 ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt
     5401 cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt
     5461 catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt
     5521 ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct aaagaacgtg
     5581 ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat
     5641 tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt
     5701 cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag
     5761 caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga
     5821 acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt
     5881 ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg
     5941 catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct
     6001 gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat
     6061 cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa
     6121 cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc
     6181 ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc
     6241 agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt
     6301 caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt
     6361 ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc
     6421 tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc
     6481 ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat
     6541 tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc
     6601 cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct
     6661 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
     6721 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
     6781 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
     6841 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
     6901 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
     6961 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
     7021 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
     7081 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
     7141 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
     7201 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
     7261 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
     7321 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
     7381 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
     7441 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
     7501 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
     7561 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
     7621 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
     7681 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
     7741 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
     7801 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
     7861 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
     7921 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
     7981 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
     8041 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
     8101 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
     8161 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
     8221 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
     8281 ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
     8341 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
     8401 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
     8461 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
     8521 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
     8581 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
     8641 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
     8701 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
     8761 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
     8821 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
     8881 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
     8941 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
     9001 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
     9061 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
     9121 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
     9181 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
     9241 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
     9301 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
     9361 ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga
     9421 acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga
     9481 tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa
     9541 agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac
     9601 attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga
     9661 acacttttgt ctcacgctct aaacacgtat aaacggcgaa agcagaagca actccttcgg
     9721 aaataagctg aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct
     9781 tggagttaaa cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt
     9841 tcttttgtac ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt
     9901 cctctttatg gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt
     9961 ttacatctaa taatcgacat aatcgtaaca tctcttcttt ttctgttttt ttctttcacg
    10021 tctttgccat tctgtttcca tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa
    10081 taatttttat atttttttga ttagacattt ctatgatttt aatatagtaa aaagagccgt
    10141 cacggaaaat ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt ttggctattc
    10201 ttattacttg atgcagggca gcttgtgtca agacttcttt acatcaaact ttttaatttt
    10261 acattgggat atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac
    10321 ggttggtcga aacggtcacg agccaagata ttgcgacttg aactcatctt ctcatccaaa
    10381 tcttctggat cgatccagcg agcgattttg tgtcccattg gagacatgat tacttcggcg
    10441 ttatactcat ttaacatacg atattggaat acttcgaatt gtagctgacc aacagctcca
    10501 atgatatatt cttccgttag atatgtttta taaagctgaa tcgctccttc ttgaactaat
    10561 tgatagattc ctttgtggaa ggatttttgt ttcatgacat tttttgcagt cactttcata
    10621 aacagctctg gagtaaatga agggagttct tcataagcaa cttttagttt tccttcgaac
    10681 aatgtatccc caatctgata attgcctgta tcatatacac cgatgatatc gcctgctact
    10741 gctttttcga cgttttctct cgaatctgcc ataaattgtg tgacattgct taatttcagt
    10801 tttttatttg tacgttccaa ccagacatcc attcctcgtt cgaatgttcc agaacagatt
    10861 cggacaaaag cgattcggtc acgatgggct ggattcatat ttgcttggat cttgaatacg
    10921 aaacctgaaa attcttcctc ataaggactt accatctgat catcttgtgt tttgtgtcca
    10981 taaggagctg gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc
    11041 aatgcagaac caaaaaatac tggtgtctga tcgccacgag caattttttc ttgattaaat
    11101 gcatctccag cctctttgat tagttctact tcttctaaga cttgttcata taaggaattt
    11161 tcagttagag gatgattttc gggaagcagt ccatcttcat ccaattctac taatcgttca
    11221 tcctgataat tttccggacg ataaaactct acacgattat ggtagatatc ataaagacct
    11281 tctagccctt tacccatacc gattggccaa ttcatcggat aagattcgat atctaatagc
    11341 tcttctaatt cttctaacaa ttccaatggt tcacgaccat cacgatctag tttgttgata
    11401 aaagtaaata tcgggatgcc acgttttttt acaacttgga ataatttctt tgtctgtgct
    11461 tcgatccctt ttgcactatc gattaccatg accgcactat ccacggccat caaagtacga
    11521 taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca agatatttac tcgtttccct
    11581 tgataatcaa actgcataac agaactagta acagaaatcc cacgttgttt ctcgatttcc
    11641 atccagtctg atttggcaaa attacctgtt tttttgcctt ttacggtccc ggcttgacgg
    11701 atagccccac caaacaatag taattgttct gtaatcgttg ttttcccagc atccggatgg
    11761 gaaatgatcg caaaagttct gcgattgtcc acttgttgct ttaattttgg attattcata
    11821 acctaactcc atttcttcca ttcaattatt tatattttct ctaggcacag attgtccgat
    11881 ctcttaaaga aagacaattt catatagggc tgtgacaaaa gccgtgtagc agtctctttt
    11941 tccgagtaaa cggcgttcta gagacagcct atcagcaata agcccaccca agccaaaaat
    12001 acgaaaagct gtttttgctt ggctgctctt attacttaat gcaggatagc tttttcacaa
    12061 cctttgtcaa ctactacaac tgaatcagta tagcatggat tcagcgaatg aaaaagccac
    12121 caaaccataa gagtcaaata tttttttgtc agctgactaa aagaaaagac gtgttgcttg
    12181 tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc
    12241 ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg
    12301 atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag
    12361 cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc
    12421 tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc
    12481 ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt
    12541 ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa
    12601 aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat
    12661 ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac
    12721 gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc
    12781 agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata
    12841 aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc
    12901 gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt
    12961 atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt
    13021 ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat
    13081 cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc
    13141 tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt
    13201 taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc
    13261 cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa
    13321 ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc
    13381 tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga
    13441 tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc
    13501 aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg
    13561 cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc cttataaaat
    13621 taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac
    13681 aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa
    13741 ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga
    13801 tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat
    13861 ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa
    13921 ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca accacacaac
    13981 aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta
    14041 gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag
    14101 cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt
    14161 cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca
    14221 caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc
    14281 cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag
    14341 cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga
    14401 ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca
    14461 aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat
    14521 tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa
    14581 taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg
    14641 ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta
    14701 caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg
    14761 atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag
    14821 actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata
    14881 ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg
    14941 ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac
    15001 aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt
    15061 agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca
    15121 cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga
    15181 aaacggaaaa atgaaattac agcggatggc gattctttct gaatacagac accaagggtt
    15241 aggaaaaata ttgattgaag aagcagaaaa ctttgctaag aatcaaggct ataatacgat
    15301 tttattaggg gcacagtcca cagctgaaac tttttacgaa aaactaggat atacagctta
    15361 cggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa
    15421 atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac
    15481 tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat
    15541 atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt
    15601 ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc attttgctgt gcatctcata
    15661 ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata
    15721 gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata
    15781 ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata
    15841 aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg
    15901 atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt
    15961 tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg
    16021 aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta
    16081 caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt
    16141 gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga
    16201 atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc
    16261 tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg
    16321 agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag
    16381 attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca
    16441 ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat
    16501 attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg
    16561 aagagctgca ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa
    16621 actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca
    16681 ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc
    16741 acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga
    16801 agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc
    16861 gtcaactgca ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg
    16921 tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc
    16981 tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc
    17041 ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa
    17101 ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat
    17161 gatctcagag atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt
    17221 ttcttgttca taccattgaa taaactgatc ttgaaattct tttgtttctt tatctgtcca
    17281 tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat
    17341 ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt
    17401 caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta
    17461 tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg ttggatcact tcttgagaaa
    17521 agcctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgt
    17581 gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg
    17641 tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct
    17701 gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc
    17761 catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat
    17821 cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact
    17881 gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga
    17941 tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata
    18001 catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt
    18061 tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta
    18121 tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa
    18181 gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga
    18241 aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat
    18301 ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc
    18361 gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac
    18421 catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc
    18481 aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct
    18541 ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca
    18601 aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg
    18661 aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac
    18721 aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg
    18781 aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg
    18841 agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc
    18901 gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa
    18961 aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag
    19021 aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc
    19081 agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa
    19141 cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca
    19201 aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg
    19261 aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta
    19321 aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt
    19381 tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct
    19441 cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg
    19501 aatatttaca gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga
    19561 tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa
    19621 taagaacagc caaacaaaaa tagctcttca tgttttatga atactgtact tgtgaccgaa
    19681 gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag
    19741 ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat
    19801 cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga
    19861 tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt
    19921 caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat
    19981 cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat
    20041 taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga
    20101 ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg
    20161 gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt
    20221 atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga
    20281 acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac
    20341 ctccttcatt tgataaccac attgtaaacg ctttattgta ttaacacaaa cattatgctt
    20401 ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag
    20461 tagacgagaa aagtacaaat agaaaacatg agaatcaaat catatgatct ttttcaaaaa
    20521 catcattcat ataaacaaat gttcaacttt ttaataacaa caattatcaa tttttttctt
    20581 acttcacaaa ctaaatacag gtcttgctgt gagccttcat ggtaaactaa tattatagaa
    20641 aggagttagt aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg ttataggaat
    20701 tgtcatatta ggctttttat cagggttgct tagcggaaat cccggaaaat attactattc
    20761 tttgcaactt ccaccatttg caccacctag ctggatattt gggccgatgt ggaccttact
    20821 gtatatattg atgggtattt ctctttattt gttacttaat cacaacaaca aaaaacagcg
    20881 aaacaatctt gtcggtcttt ttgtgattca gttcattttt aattttatat ggtctgctct
    20941 attttttaat ttaagaaata ttttcattgc agcaatcgat attacacttt tagttatttt
    21001 cttgtcagtg cttatgtatc agttgtggct gcatcatcgc ctgacaatgt ggctcatgat
    21061 tccttattat ttatgggtac tattcgcaac tctattgaat tattcaattt attttttaaa
    21121 ctaagcgcta aaaagagacg tcctgtaatt taacacatag aaaaagtgcc acctaagtta
    21181 gattttttgt ctaatttagg tggcactctt tattaaccaa actatttttt tctagtaccc
    21241 acttgttgtt ccatactcat tattctggtc ataaccataa ccattttgtt cctgtccagc
    21301 gtaaggatca taactttgag tatcttgact tggtgtatag tactgacttg tatcttctgt
    21361 ttgctgataa taggtattcg gatcttggta ctgatcatat gtgttttctg aataggaaga
    21421 ataatcggtg tacccttcac tattcgatcc atcaccgttt gtcgaatcgc catcttccga
    21481 atcatcaaag aacatcattg aagcattctc atttttcaaa ttaggtaatg tatcgcttgt
    21541 ttttatattt aattgtttct tcagttcatt ttggatcgaa agaagatcat tttttcctaa
    21601 aatttgatag tagatatcat tgatcatttg tccttcgcct tgcaactggt cttgtttgat
    21661 tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc atgtcatccc aatctaagtc
    21721 cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta taattgctta cgccatctaa
    21781 acttaaaact ttattgacga ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat
    21841 atcgccttct ggatcttcgt gtcgcattcg agaataggct aaaccttttt ctccattcaa
    21901 tgtttgttca cctactggta catggacacc atccaaagta aagtcaattt tattattcac
    21961 tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca tccaaattga tcgttacata
    22021 atgatcaata ggaatatcga gcagttgctc aattgaatcc atggccattt cgacaccacc
    22081 aaatgcgtag gcatgattta atttatctac cgttccataa cctacgatat tcgtataaat
    22141 atctcggtcc aaactgatga tcgttgattt tttttgtttc ggattaactg ttactaccat
    22201 catcgtatca gaacgtcctt gttccgttct tcctaatcca ccggtatcta aacctaatag
    22261 caaaaccgaa aaaggttctt tgtcatcaat gcttactctc tcagaacgtt tggaggatat
    22321 tcgattagag tttttagaaa ttttgtttac tgtttggctc gcttctccca taaatctgat
    22381 tccgtatgct gataatccgg caatcagcaa aacgaatacc cctaacaagg ttaatagaac
    22441 tttttgccat cgcttcataa tacacctctg tttcacattt tctaaaatat tgtctcatat
    22501 aactgttcaa tcagcaaatt ca
//
LOCUS       DACDHJ010000029        21674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDHJ010000029 DACDHJ010000000
VERSION     DACDHJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.029"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..325
                     /locus_tag="IV223_000858"
     CDS             50..325
                     /locus_tag="IV223_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8711162.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            331..1098
                     /locus_tag="IV223_000859"
     CDS             331..1098
                     /locus_tag="IV223_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8711163.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1266..3095
                     /gene="typA"
                     /locus_tag="IV223_000860"
     CDS             1266..3095
                     /gene="typA"
                     /locus_tag="IV223_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8711164.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3356..5128
                     /gene="recQ"
                     /locus_tag="IV223_000861"
     CDS             3356..5128
                     /gene="recQ"
                     /locus_tag="IV223_000861"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8711165.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5242..5544
                     /locus_tag="IV223_000862"
     CDS             5242..5544
                     /locus_tag="IV223_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8711166.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5547..6710
                     /locus_tag="IV223_000863"
     CDS             5547..6710
                     /locus_tag="IV223_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8711167.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6736..10164
                     /locus_tag="IV223_000864"
     CDS             6736..10164
                     /locus_tag="IV223_000864"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8711168.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10185..11309
                     /locus_tag="IV223_000865"
     CDS             10185..11309
                     /locus_tag="IV223_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711169.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11299..11736
                     /locus_tag="IV223_000866"
     CDS             11299..11736
                     /locus_tag="IV223_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8711170.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11720..12058
                     /locus_tag="IV223_000867"
     CDS             11720..12058
                     /locus_tag="IV223_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8711171.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12177..12737
                     /gene="rsmD"
                     /locus_tag="IV223_000868"
     CDS             12177..12737
                     /gene="rsmD"
                     /locus_tag="IV223_000868"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8711172.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12734..13225
                     /gene="coaD"
                     /locus_tag="IV223_000869"
     CDS             12734..13225
                     /gene="coaD"
                     /locus_tag="IV223_000869"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8711173.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13215..14258
                     /locus_tag="IV223_000870"
     CDS             13215..14258
                     /locus_tag="IV223_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8711174.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14417..15145
                     /locus_tag="IV223_000871"
     CDS             14417..15145
                     /locus_tag="IV223_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8711175.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15261..15506
                     /locus_tag="IV223_000872"
     CDS             15261..15506
                     /locus_tag="IV223_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8711176.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15503..15721
                     /locus_tag="IV223_000873"
     CDS             15503..15721
                     /locus_tag="IV223_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8711177.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15857..16396
                     /locus_tag="IV223_000874"
     CDS             15857..16396
                     /locus_tag="IV223_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8711178.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16389..18662
                     /locus_tag="IV223_000875"
     CDS             16389..18662
                     /locus_tag="IV223_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8711179.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18700..19746
                     /gene="holA"
                     /locus_tag="IV223_000876"
     CDS             18700..19746
                     /gene="holA"
                     /locus_tag="IV223_000876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8711180.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19835..20089)
                     /locus_tag="IV223_000877"
     CDS             complement(19835..20089)
                     /locus_tag="IV223_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8711181.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20489..20758)
                     /locus_tag="IV223_000878"
     CDS             complement(20489..20758)
                     /locus_tag="IV223_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711182.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21228..21656)
                     /locus_tag="IV223_000879"
     CDS             complement(21228..21656)
                     /locus_tag="IV223_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8711183.1"
                     /translation="MLLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPWLQGKGINIG
                     VTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGVGLIASDPQI
                     TVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
BASE COUNT         7264 a         3688 c         4585 g         6137 t
ORIGIN      
        1 ccacttccta tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata
       61 tcagtatcca cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg
      121 ggaagatctg gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta
      181 tcagcagttt aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt
      241 tgaaaaagct tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa
      301 caaaactttg agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc
      361 tggcttgaag aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa
      421 aaatcaaatc gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc
      481 gaaaaaatcc attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat
      541 caattatcgg atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat
      601 tttgtgattg aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga
      661 aagcttggtt tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata
      721 ggcgtgtata aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc
      781 ttacttggtg tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg
      841 gaaagcagca tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg
      901 aaaggaaacc acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat
      961 gtcttattgg aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt
     1021 gaaaaaaggg aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt
     1081 ttgaaaaaag aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca
     1141 aaagattaat tttctcgaaa aaatgaaaac attttactat atttcatgaa aaaagtctgc
     1201 tataattagc agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag
     1261 ttacattgaa ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg
     1321 gaaaaacaac attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc
     1381 aattgcaaga acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt
     1441 tagccaaaaa cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag
     1501 gacacgcgga cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt
     1561 tggtcgtaga tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac
     1621 tggaacaaca tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc
     1681 ctgagcatgt cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc
     1741 agttagactt tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg
     1801 atccagcaga tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc
     1861 ctgctcctgt cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca
     1921 acgattatgt aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag
     1981 accaagtagc attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat
     2041 ttggtttctt tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg
     2101 ctgtctcagg tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag
     2161 atgcgttacc tatccttcac attgacgaac caacattgca aatgactttc ttagttaata
     2221 attcaccatt tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt
     2281 tgatggcaga acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg
     2341 catggacagt ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc
     2401 gtgagggata cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg
     2461 taaaatgcga accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg
     2521 ttatcgagtc cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg
     2581 gacaaatccg tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat
     2641 tcctttcaat gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa
     2701 tgcttcctgg tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa
     2761 aagcaacgac ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag
     2821 gtacagaagt atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg
     2881 tcaacatcac gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat
     2941 ctgtcatcaa aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg
     3001 atgaatattg cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa
     3061 atgctcgtga aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac
     3121 cagcttgtta taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt
     3181 catctttgtc aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta
     3241 aaaaacttat ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat
     3301 caacttgtcc ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa
     3361 cgacttatta aaacaatact ttggttatga tgagtttaga cctggtcaaa aagaaattat
     3421 ccagaaagtc attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc
     3481 aatctgttac cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt
     3541 aatctctttg atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt
     3601 tatcaatagc tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca
     3661 gcagtaccgt ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc
     3721 aatgaagcga tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg
     3781 ggggcatgat ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa
     3841 tcgtcctgta attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa
     3901 aagactttta aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct
     3961 acgatttcaa gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat
     4021 caacaaaaat caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta
     4081 tcatttgctt aaaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa
     4141 tgagcgaaca gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac
     4201 aaatgcattt ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat
     4261 tcctggctct ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc
     4321 cagtgaagcc atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca
     4381 gcagtcccaa agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac
     4441 agaatacgtg tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac
     4501 aagcagtccg tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac
     4561 gacagaagca cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca
     4621 aatgctgatg aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg
     4681 acatttatct acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat
     4741 tgaatatttg atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa
     4801 agtaacagaa agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc
     4861 caagaaagtc cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga
     4921 actgcgaacg gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc
     4981 cacattgaaa gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa
     5041 aggagttggc caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa
     5101 tgcggactct aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca
     5161 actggttaaa tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat
     5221 tagaatggga ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta
     5281 aatgatgaag cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc
     5341 tcacaatgca aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa
     5401 gtcggttttg ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt
     5461 gagctagaac gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa
     5521 aaagaagttg gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag
     5581 actatagcat cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata
     5641 gttctacgtc gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc
     5701 agagtatttt ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag
     5761 atgttttgaa aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc
     5821 tgattgttgt gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag
     5881 gattctcgat ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga
     5941 ctttatctaa aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac
     6001 ttgcaatggt tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg
     6061 cagttatctt tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata
     6121 ctttgattgt aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata
     6181 tcacaaacgg aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt
     6241 accaaaatcc tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg
     6301 ccatgttcaa cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat
     6361 ttttacaaga agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga
     6421 ttatggggat tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag
     6481 gtatccgttc aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt
     6541 tgatccaagt atttgtcaat cttggaggaa ttactggcgt tattcctttg acagggatca
     6601 cgttcccatt tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct
     6661 ttgtcctcaa catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga
     6721 tgtaggagtg gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt
     6781 gtatttcgtg cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat
     6841 gaatattccg tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa
     6901 ccgattgatg cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct
     6961 gacgctattc atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc
     7021 gaagaagaag gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat
     7081 aaaatcaaag caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat
     7141 ggaccaatcg cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt
     7201 atgatcaagg cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag
     7261 agtgcaagag agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat
     7321 gaagtgtatg ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg
     7381 gatacgcatg gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac
     7441 caaaaagtag tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc
     7501 tgccaggcag ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa
     7561 tttcttgttg aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa
     7621 catacgatca cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct
     7681 caaggtttgg atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat
     7741 ggttcagcca ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac
     7801 acagggaaaa tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga
     7861 aatgcttacg cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt
     7921 acgcatggtg ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt
     7981 agaatccgtg gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa
     8041 ttccaatcag gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt
     8101 cctcgactac gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat
     8161 ggttttccag gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa
     8221 gatctaatca caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc
     8281 gatgctttgg tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt
     8341 agagacgccc atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt
     8401 gcacgtttga ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca
     8461 acatttgatg tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt
     8521 cgttctttaa tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg
     8581 tattctaatt atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga
     8641 atcgatgtat ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc
     8701 caagctgtac gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc
     8761 aacgatccaa gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta
     8821 gaacaactgg gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag
     8881 gcagcttatc gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat
     8941 actcatgata ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta
     9001 gacatcgttg acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc
     9061 agcttgtatt atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca
     9121 cagaaattaa accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc
     9181 ttgaatgctc ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat
     9241 ctacagcaac aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg
     9301 tatcatactg tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta
     9361 gttggagata tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa
     9421 aaaggcgaaa cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg
     9481 caacccgttg gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca
     9541 ttgaatgaac gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa
     9601 ttggcagaaa aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat
     9661 ccgcaagtct ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg
     9721 gacacaccaa catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa
     9781 cgcggaaaaa cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat
     9841 cgcgtactgt tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg
     9901 atcatcagta cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga
     9961 gcaacgatgt ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa
    10021 ggtgatacgt tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt
    10081 gacggagaag tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt
    10141 ttgattgaag tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt
    10201 tgttttggct gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc
    10261 cgccaaaact tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt
    10321 ggaaatacca ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc
    10381 agctggaaaa ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa
    10441 caagatatta tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca
    10501 gagtcacaac tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag
    10561 ggatgacgat gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt
    10621 accatgatac agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa
    10681 ttgcttttga taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat
    10741 tttcagttgt gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg
    10801 gtgaaggcct tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg
    10861 ttaaagaacg aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca
    10921 tatacaatca aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga
    10981 caaggcctga ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca
    11041 gtgctcaaac gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta
    11101 aacaaaaatt agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt
    11161 cgtttttata ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg
    11221 attctgtagg cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg
    11281 gagaaagtag tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc
    11341 ctccaagtcg ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa
    11401 cagtcaatgg aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa
    11461 gaagcatttg aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa
    11521 cgagctgttc gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga
    11581 tatagtgaaa cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa
    11641 gtttctaatg aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt
    11701 ggatgcggag gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg
    11761 cgggctgatc gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt
    11821 gttgcattat gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt
    11881 ggaagaaaca gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta
    11941 ccgaccggat atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga
    12001 cgaaggtttc gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa
    12061 atctcttatg agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat
    12121 tgtccaagta tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc
    12181 ggataatatc aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca
    12241 gaccaactac agataaagta aaagaatcca tattcaatat gattggacca tattttgatg
    12301 gagagacagt attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta
    12361 gaggctgcag tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag
    12421 aaaacatcga aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca
    12481 aagcaatcga acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc
    12541 catatgcaaa acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt
    12601 ctaaaaccgc agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg
    12661 ctggattcca tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa
    12721 aagagggaaa tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg
    12781 tcacttagat acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt
    12841 tatcaatact tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa
    12901 agctgtttct catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga
    12961 aacagcaaaa gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt
    13021 tgaatatgaa cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt
    13081 cttcttatta gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact
    13141 gcactttggc ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg
    13201 aaagcgtgaa tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg
    13261 gaatgttgat cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag
    13321 ccacgatcga tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt
    13381 ctttctctct tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta
    13441 aaatgacccc ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg
    13501 aagaatataa tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac
    13561 aagcgttaaa acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga
    13621 gtgtagagaa aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag
    13681 tagacgggaa aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag
    13741 ttggacaaac aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg
    13801 atctgatcga gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata
    13861 ccgagatcga atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag
    13921 cgggattgat gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg
    13981 gtaagcaaat tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag
    14041 gaatcgataa aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag
    14101 atgatacgat ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag
    14161 aagcaaaagc tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag
    14221 tacaagaagc gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt
    14281 tgtgacacga gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa
    14341 aaacaaaaga aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact
    14401 gtttcttcaa ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa
    14461 aagaacaatg atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg
    14521 gatgtctatg atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct
    14581 attttataga gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag
    14641 tggagaagat ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat
    14701 gtacaccgat atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga
    14761 acgggtctat gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg
    14821 aatcaacttt tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga
    14881 agagctgcca gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga
    14941 ttccgagcct cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc
    15001 tgggatcgga tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt
    15061 ccagaaagtg gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact
    15121 aaaaaacttc gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg
    15181 gacgctaatg gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt
    15241 atttttggat aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga
    15301 atgtctatct ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca
    15361 ataacagtca agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata
    15421 aagtatttat tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa
    15481 cgtaccaaaa aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca
    15541 tgctttcttt atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa
    15601 gttgatcaaa gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt
    15661 atcgattttt atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta
    15721 atggatgacg gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt
    15781 cgttatattt aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa
    15841 cggaggaata taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa
    15901 agttactgat aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc
    15961 agtactgtta tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg
    16021 agaaaaaagg atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat
    16081 agatgaaggg tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa
    16141 tgcactccta cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac
    16201 acattttcca tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa
    16261 ttatctccat gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt
    16321 atctgtacac caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac
    16381 aggaaaacat gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa
    16441 ttacgtcgat gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc
    16501 ttgccggaat ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta
    16561 ttttgtgtag cataacggta tccatttctt ataggctaca agagtattca tttttagagg
    16621 aaaaaacaac atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt
    16681 ttttgacaat ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag
    16741 taaacacaga agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga
    16801 ctgctgaagg cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag
    16861 aagcttactt caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga
    16921 tcaccatgca aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct
    16981 ttgtccaatc tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct
    17041 ataaagatgc aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc
    17101 tgttcagtct atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc
    17161 gacgtatcgg atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt
    17221 atgtttctat tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc
    17281 gattgctagt caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct
    17341 tttggcttct tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt
    17401 ttttcatgag ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg
    17461 cctctcacgt cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt
    17521 ggcctatttt aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc
    17581 ttcctggtgt gttatttctc acactcttca atcgctggct gccggaaact ttacttttgc
    17641 tgatagaaga gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc
    17701 tcattatagg acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt
    17761 gtatggatag gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt
    17821 ttattttttt gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg
    17881 gtcaaggaga cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata
    17941 ctggaggtca gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg
    18001 ctgaaagaaa tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca
    18061 tcttgacaca cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta
    18121 ctatcaaaaa aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt
    18181 tatctgaagc tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca
    18241 gcggttatta tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag
    18301 attcgattgg tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga
    18361 atcaagcaat ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca
    18421 aattaggtca tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag
    18481 caaaacatgg gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag
    18541 tattggagac actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga
    18601 tcacctatcg ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact
    18661 agtaattctc atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata
    18721 caagcagagt tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga
    18781 acagaaaagt ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag
    18841 ggcgaagatg acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta
    18901 gtagctgaag cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat
    18961 ccttattttt taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg
    19021 acggcttatt tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa
    19081 aagttggatg aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat
    19141 gtccaacagt tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc
    19201 tcaatcaaaa tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta
    19261 tccaaaatga tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc
    19321 acggtaagag aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg
    19381 acccaatata ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt
    19441 atgcaagggg aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt
    19501 ttacagacaa aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg
    19561 aaaatccatc catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat
    19621 ttgctggtac agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa
    19681 atcgaaaaag aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat
    19741 cgataagaag aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt
    19801 tgacaaataa aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac
    19861 gagatttgtc gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa
    19921 tagctttaac agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat
    19981 cttcaaattt tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag
    20041 cgttttcgct tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct
    20101 ccgataactt aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa
    20161 aggcagcgtg ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag
    20221 gatttttttt cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt
    20281 gggaggcaat agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa
    20341 tgggaatgtt tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt
    20401 gaaaagggat acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt
    20461 ataaccagta tcctttgatc agtaaaggct attctggtgt accgattatt ttaatgtatt
    20521 catcgatatt ttttatcaac atttttttta tgaccaactg gttccgacgg ttccatttgc
    20581 cacttacttc cagcacatat ttatttaggg gcaaacgtaa gatatatttt gccaaatagc
    20641 gatctttaag caggccattg atcacacggt cctcaagctg caagttgatc ccagtcagca
    20701 tcattggctg ttcttgtaaa acgtacgtat caattaaaag accttcaaaa gtttccacaa
    20761 gcgtcttcct ttcttttcaa agtaacctga aaacaggcac taattcttcc attttgatat
    20821 tcgttgaagt ggatgcgatc attctttctt ttgtccactg taatctagta taacaaaaaa
    20881 gagcagaaga gccaaaaaga aaaaagagca tcgaattcta taggaagtct cattggcacg
    20941 gcctaaatta ttacaaaaca ttgattaaaa aaagcccctg catcgcagga gcatccccta
    21001 tatcgtttta ataggtcaat caaaaagcag tatcaaacac attttgtatc aaatcaggaa
    21061 ataatagata agctaagccc agaatcaaaa gtaccgctag aagaaaccag ccgattgttc
    21121 ctatgaaata ctttaatttt tcgaacattg tatcacctcg ttgtcagtat atcacggatc
    21181 agtaaattgg gggtgttaag aatctcctaa aatagacaaa atttgttcta ttttcctaaa
    21241 gcaaatactt gtaagataca gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt
    21301 cctcttaaga agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga
    21361 tcagaagcaa tcaatcctac acccttggaa gataagacag ccactagccc accgcaaaaa
    21421 acagctaccc agccaatagg ggatttaaag gcttctaata gatcacgtag accgatttct
    21481 cctgttgcga tagggattag aatagccgct gtgatgatcg tcaccccaat attgattcct
    21541 ttgccttgaa gccaagggaa aaaggaacgg gaggctggta tgagcttcat aatcaaaaca
    21601 aaagctgccg ctatcatcag actttgattt ttagctaaaa ttgctacaag taaaataatt
    21661 cctaaaaaaa gcca
//
LOCUS       DACDHJ010000030        20384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDHJ010000030 DACDHJ010000000
VERSION     DACDHJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.031"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IV223_000880"
     CDS             complement(<1..456)
                     /locus_tag="IV223_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8711184.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IV223_000881"
     CDS             complement(561..1424)
                     /locus_tag="IV223_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8711185.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IV223_000882"
     CDS             complement(1620..3719)
                     /locus_tag="IV223_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8711186.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IV223_000883"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IV223_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8711187.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IV223_000884"
     CDS             complement(5072..6913)
                     /locus_tag="IV223_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8711188.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IV223_000885"
     CDS             complement(7095..9488)
                     /locus_tag="IV223_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8711189.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IV223_000886"
     CDS             complement(9595..10455)
                     /locus_tag="IV223_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8711190.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IV223_000887"
     CDS             complement(10514..11035)
                     /locus_tag="IV223_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8711191.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IV223_000888"
     CDS             11355..12074
                     /locus_tag="IV223_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8711192.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IV223_000889"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IV223_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8711193.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IV223_000890"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IV223_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8711194.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IV223_000891"
     CDS             16434..16790
                     /locus_tag="IV223_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8711195.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17031..18035)
                     /locus_tag="IV223_000892"
     CDS             complement(17031..18035)
                     /locus_tag="IV223_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8711196.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18201..19052
                     /locus_tag="IV223_000893"
     CDS             18201..19052
                     /locus_tag="IV223_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8711197.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19069..19314
                     /locus_tag="IV223_000894"
     CDS             19069..19314
                     /locus_tag="IV223_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711198.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19501..20205
                     /locus_tag="IV223_000895"
     CDS             19501..20205
                     /locus_tag="IV223_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711199.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5764 a         4184 c         3566 g         6870 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    16921 acttttgtct cacgctcttt ttttgttata ttcgtctttt ccgatccttc tcttgacaca
    16981 attcatttaa ctgatcactg gtacttgaac acctaatatt ctagtcattt ttataaacga
    17041 ttcaatactt catccacatt tttgatcatc acggatatcg taccatccgg attattcaca
    17101 aattcaatca aatctgggtt gcgatataca tctactggta cgatcagttc gatcccgttt
    17161 gatagcttga atttttgttt tccaaatttt ttctcagaaa tttcttgtac ttcttttaca
    17221 ggaggtgctt ctgggacaaa tccttgctct tgtacttctt ctttaaaagc catttttgcc
    17281 gtgatgtttt ccttgaagac tttttccgca atcatctcat gatccaaccg acctttttct
    17341 tctatcgtat catagacagc ttctttcaaa tcagccatga tatcaaattc ttctgtctca
    17401 aatttttttc caagatgctt ggctactttt tggatcactt ttacattttc ttctaaagaa
    17461 ggtgcaggaa cactttcgat cactcttcca gaaaaataaa gctctttttt gccagaaaac
    17521 tcatattttt tttcaattaa ttcgtaggtc caatcagtca aattcacagc gaatgcttca
    17581 tctgcttttt gtgttttcgc ccctagaatt gctcgattca aaatcaagcg gttgtcgatc
    17641 cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac gataattgac tttcaataaa
    17701 gataagtaca tcactgtatc taattcatac aagacaacaa atacatctgc actaggtgct
    17761 tcttcacttt cttggtagat atcgaaccac cgctgaacga ttgcttcact tgcttccaca
    17821 aaattttctt gagcgatttt aagtgattca acgatcatgc tttcttctgc tagttgtcca
    17881 gtttttgttt gagcagaaga gattttttgg atcttctttt gaagaaaatc acgaatatat
    17941 tcttttgtca gatccagctc tttttgtgaa aagactggag aaccagtttc tctgtcaata
    18001 atatgcagaa ttgctttttt taaatagata tccattttct cattcccttc tgtcttagtt
    18061 tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt tcttttaaac aaaaatttca
    18121 agagtagaaa caaaatggat tctttttttc gaaaaatgcg ctacacttat agttgaacga
    18181 ataaaaaagg agctgaaagt atgaattcat taacaagcac ttatcgtcta tcaaatggtt
    18241 acgaaatccc ggtagtagga ttcggcacat ggcaaacacc tgatggggat gtcgctgttt
    18301 cctctgttaa agaagcgctg gctgcaggtt atcgccatat cgatactgcc caaggataca
    18361 agaatgaaga aagcgtcggc caagcgatta aggaaagtgg aattcctaga gaagagatat
    18421 tcttgacaac aaaattatgg aatgcaaacc acagctatga acttgtgatg tcttcttttg
    18481 aagaatcatt gaagaagctc caaacagatt atttagatct gtttttgatc cactggccaa
    18541 accctgttgc ttttcgtgat aattgggaac aagcaaatgc agatacatgg cgagcatttg
    18601 aagaactgta tgaagcaggt aaaatcaaag caatcggtgt gagcaatttc ttaccccacc
    18661 atctagatac actagcaaaa acagcaaaaa ttatgccgat ggtcaatcaa gttttccttg
    18721 caccaggcga attacagaca actgtagttg agtatgcaaa aaaacatgat atgatcctcg
    18781 aagcatatag tccattaggc actggtaaaa tcttcgatgt gcctgaaatg aaacaaatcg
    18841 ctgaagcaca tgacaagacg attgcacaag ttgctttgcg ttggtcgctg caacacgaat
    18901 tccttcctct tcctaagtca gtgacaccaa gcagaatcaa agaaaatact gaattatttg
    18961 attttgaatt aacagaagaa gaaatgaaac aaatcgatca gttagatggt gtagtcggca
    19021 aagcaaaaga cccagatact acccaatttt aagtcgaaag gtgattttat ggatccaatc
    19081 gaacgcttga attctctctc ggaagaagtt acccagactt ttcattccga ttttgtcttt
    19141 ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa actggacaca tgatcaaatc
    19201 atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg tcaaaccctg gcacgaacat
    19261 gaagtcattt attcacctga actacctgtt tttgccttga taccgaaaaa atagaaagaa
    19321 tggaacggat tatgtatata tacataatcc gttccatttt ttatttttct ttcaaaaaac
    19381 gggaaaatac gcaaattttt ctgttgtaat ttgcgtatac gcaagttttt ttgcattact
    19441 ccttttcatt tggctgtttt tttcttataa tcgaaagggt atgagcgaag gagagaacat
    19501 atgaagaaaa aacagaaaca aaaattatta aatcaattcc gtccttcatt ggatggtgta
    19561 agaggacaat tattccgttc tctagaagaa gagagcttgc gacgttatag tttgccgtta
    19621 caagtcatgc ttgatcccaa aaaccgtcaa atcttaacga tcggattagc tgggaaaaca
    19681 aatgaagata cacggatcaa cgtgccaatc gatgacaact tcaatactgt attaaagaga
    19741 attcgttcag gagaaaacgg gattttcgaa cgattccgtg acaatcttct tattgaaatc
    19801 gtgtcttact ggaatgatca acgattgaaa aacgatgaac caactgcgca agctgttgcg
    19861 acaccagtta cagaagaaac agtcagtgtg ccagaagaaa aaacggaaaa accaaaagcg
    19921 gacacaactg aaaatacacg ctcaactgat acgaatgccg ctttaagttt tgctgatttt
    19981 tcaaaagccg ttgctgagta tcctaaattt tacacagaac aaaatgaaca tgaagtagtt
    20041 attaatgaaa aaaatgctga tgaacctcgt aaattagcaa ctatttctat gactactgaa
    20101 aatgagttcg ttatcgaaaa agcactcgaa cggaaataca aagtcaaatt gactttgatt
    20161 cctttgatcg aacaatttgc agcgacagcg attactgatc gataaaaatt ctatttgtca
    20221 taaaaggctg ggatttaaaa tcccagcctt tttagtcata taataatttc tacagcaagt
    20281 aaaaagaaat tccttctaac atcaggctca aatatcagct tgacctgtcc aacaaatcac
    20341 ctatgatcct acaatgttag gcgacttctc ttctacctga agat
//
LOCUS       DACDHJ010000031        19610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDHJ010000031 DACDHJ010000000
VERSION     DACDHJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.032"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..258
                     /locus_tag="IV223_000896"
     CDS             4..258
                     /locus_tag="IV223_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8711200.1"
                     /translation="MDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRSSI
                     DRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            272..586
                     /locus_tag="IV223_000897"
     CDS             272..586
                     /locus_tag="IV223_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8711201.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(1316..1870)
                     /locus_tag="IV223_000898"
     CDS             complement(1316..1870)
                     /locus_tag="IV223_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8711202.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1996..3924)
                     /locus_tag="IV223_000899"
     CDS             complement(1996..3924)
                     /locus_tag="IV223_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8711203.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(4230..4946)
                     /locus_tag="IV223_000900"
     CDS             complement(4230..4946)
                     /locus_tag="IV223_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8711204.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4947..6770)
                     /gene="glpO"
                     /locus_tag="IV223_000901"
     CDS             complement(4947..6770)
                     /gene="glpO"
                     /locus_tag="IV223_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8711205.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(6784..8280)
                     /gene="glpK"
                     /locus_tag="IV223_000902"
     CDS             complement(6784..8280)
                     /gene="glpK"
                     /locus_tag="IV223_000902"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8711206.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(8416..9876)
                     /locus_tag="IV223_000903"
     CDS             complement(8416..9876)
                     /locus_tag="IV223_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8711207.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9866..11161)
                     /locus_tag="IV223_000904"
     CDS             complement(9866..11161)
                     /locus_tag="IV223_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8711208.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(11493..12170)
                     /locus_tag="IV223_000905"
     CDS             complement(11493..12170)
                     /locus_tag="IV223_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8711209.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(12200..13291)
                     /locus_tag="IV223_000906"
     CDS             complement(12200..13291)
                     /locus_tag="IV223_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8711210.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(13392..13826)
                     /locus_tag="IV223_000907"
     CDS             complement(13392..13826)
                     /locus_tag="IV223_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8711211.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(14410..15867)
                     /gene="ascB"
                     /locus_tag="IV223_000908"
     CDS             complement(14410..15867)
                     /gene="ascB"
                     /locus_tag="IV223_000908"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8711212.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15864..17759)
                     /locus_tag="IV223_000909"
     CDS             complement(15864..17759)
                     /locus_tag="IV223_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8711213.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17817..19592)
                     /locus_tag="IV223_000910"
     CDS             complement(17817..19592)
                     /locus_tag="IV223_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8711214.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT         5570 a         4220 c         3304 g         6516 t
ORIGIN      
        1 gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca aattcgtggt
       61 atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca attaagcgct
      121 gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct gaaaaattgc
      181 tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca agcgatcaag
      241 atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata ttgatgaatt
      301 agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt ctccagaaac
      361 gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact ctgccaatgc
      421 agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta tgcgtgctaa
      481 tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag gtgtccttga
      541 tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag tagaggttgc
      601 gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct acttctggaa
      661 aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga acagccaaac
      721 aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg
      781 aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt agagaaaaaa
      841 acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag gtcagctgat
      901 tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta ttaagaaaaa
      961 ttttgtgaaa atggatttct acatttttgc ataaatttgg cttaataccg agaaactggc
     1021 ttttgaatac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
     1081 aacaaacgga aaaatagctg ctcatatttt gccgtttttt gagctaatac cgaaggtcag
     1141 ctgattgaac accgtttacc aagaggttgt gagcctacca tttaactccg aatcacaaga
     1201 aataatttca aaaacaactt ctcctgtttt tggtttggtt ggcttattat cgagaagtag
     1261 ctactgggag tctatttatc aaaaaaagga atgcgacata ggtcacaccc cttttttatt
     1321 ttctttgaaa catctttcgg tactcgacag catacaagac aaaagattgt aaaagaccgt
     1381 aagtgatgac ggcaaaaatg actctcaccc acagcgttgg taattctggg aaaagcagca
     1441 aaaggatcat gccaacatag atgacagcag tcgctacttt gccataccac ttcgctccac
     1501 ttaatttgat atttttagat agcccgatta atccagctac aaacataaag ccttctttta
     1561 tcaagaatag accaaacaac agccaaagcc acggtcgata ccatgccata gacaaaatca
     1621 gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac cttccctaag tctgttacct
     1681 gattatatct ccgagcaatg accccgtcta gataatcaga caaacctgag acaacaatga
     1741 ccgctcctgc tatataaaat gactgtctca gcacaaaata aagataaatc ggcagcaaaa
     1801 aaatacggaa tatcgataaa agattcggaa tcgtttgtat ttctttacgc cagtctgtgc
     1861 ttaatttcat tttatcgccc tacctcctca gcactctttt ctttttattt tagcttacac
     1921 ggggaataaa accaatgatt tcactgtttt tatttttctt tacagccact gatattatga
     1981 gctaatttga gataatcact tgctgttttc atcgtttact cttctttctt cctgtcgttt
     2041 gcgcatttct tttactagta ctttatgagc tttttttgat cctcgctcta ggtcacgctg
     2101 ccgtttacgt gttttataat cgataaacag actgtctaaa tcgtaacctg tcgggtacag
     2161 atcttctgct gaacgcacca gacgaacacg ctgacgaggc acgtggatta tttttttgtc
     2221 taaatataca tcaatcgtat cttcacctat atcttcataa actaacgctt ctttttggta
     2281 ttcattgaca aatatccgat caccttttgc aaagagctgg cgttcttttt tattctcctt
     2341 cctatttgta actgctgtct taggaagaaa ttcttttttc tgtgtccgat aggctttttc
     2401 cgccaagtaa cgctctgctt gatggatcaa tttcatcgac atatccattt tatgagcaat
     2461 ccacaaagct tggctttctc ctgtttctcc aacccgcaac tgatatttag gacgaagtgc
     2521 ttcccgatcg aaagccatag cagccggcac aaaatcttca tgatctccgg cgaatttctt
     2581 gatttctcca taatgggtcg tggcaatgat caatgcccct tgctcataca tagattccat
     2641 gattgcgatc gctagtgcag ccccttcatt tggctctgtt ccactgccaa tctcatctaa
     2701 aagtaccaac gtattccttt ttatcttttt caaaatagca gcgatgtttt gcatatgacc
     2761 agaaaatgta ctcaacgcat tttctaaatt ttggtggtca ccgacatcga cgaaaatctc
     2821 atcaaagaca gcaagctctg tcccttcttt tgccggaact tgcataccga acatcgccat
     2881 caaagtcaat aaaccgactg tcttcaacac gactgttttg cctccggcat ttgctccggt
     2941 gataatcaag cctcgatagt ctttaccgat ttcaacatcc aatggtaccg catgctcagg
     3001 taaaaatgga tgtctgcctt gcttgataat gatatgttcc gatttattga ttctaggtgt
     3061 gcttccattg atttctcggc taaattttgc ccgagcaaag atgatatcta acaccgtgat
     3121 cgtttcgatc aataaatcga tcacttcttc gttttctgcg atagcaccag tcaatgccgc
     3181 caggacttga tattcttcac tgatttcttc agcttttaac aactgatagt gttcattcaa
     3241 tttagaaatc acagttggtt caataaaaac cgtcgttctt ttatttgatt catcgataat
     3301 cgttccatct attttatttt tatagctggc tttgatagga atcgtatagt attcgccttt
     3361 ttgaacaatc attgcttctt ggatcatttc tttgtttttc ggatgacgca agaatttcag
     3421 taattttgac tggatttctt tttcgattag ttgtaattgt ttgcgtactt tacgcaaatt
     3481 acgtgacgcg tcgtcactga ctttattatt ttttattttc tggtcaatca actcttctac
     3541 atttatcaaa tcaggaagat gtttgctata agcaaacaac aagggtgtct ggtattgatt
     3601 cttatcaaaa aactttgtga tcatccgact gcttctaaga aaatccgcat attcgatcaa
     3661 atcggttggc tgcagaatca accctttttt gacttgttct gtcagtgcat cgatcctcgg
     3721 caaccccata aatggaacat gctgactgct ttctaagatc aagcgtgctt ctcttgtttc
     3781 ttcttgacga tccaagacag tttgtaggtt tgtcgagacc gtcatatcgc tgattcgttt
     3841 tttgctatag tttccaattg ctcttgcttg gatttccttt ttgatccgat caaattgtaa
     3901 ttgactaatc atttcgttgt ccatattctc tccttttctt gaagagatac ctgtcccaaa
     3961 ataaataagg gcctagagat aaataaaagg atagaacaca gcttaaaaaa gtcatgtcct
     4021 atccttatta gaatacaaag atattgtttt cctgacaggt accgtttcat aagcacacca
     4081 aataaacttt gtttattggc tattcgactt actgattaga taccagctac actcacaaaa
     4141 cataccccat tcttctaggt cttatttatt ttccacttaa ctatagcaag acacattttg
     4201 gttgtcaaac tttagtatgt tttatagatt tacaccatta atgaatagac acctaccgcg
     4261 ataattgctc cgatcattgg acctgcgatt ggtacccatg aatatgccca atctgaagtt
     4321 cctttattag ctattggcaa taccgcatgt gctaatcgag gaccaaagtc ccgagcaggg
     4381 ttgattgcat atcctgttgg ccctcctaat gaaagaccga tggctaagat caatccacct
     4441 acagcgaata cgttcgttcc aggtgcaaat tcgttttgtc caaaagctag caacccaaga
     4501 actaatacaa atgtaccaat taattctgtg atcacatttg ctggataatt ccgtatagca
     4561 ggacctgttg caaacgttcc taaaatcgcg ctttcatctt tcgttgcatt ccaatgtggc
     4621 aaataagaaa gccaaacaag aatggcccct gcaaaagctc ctaaaacttg cgcaatgatg
     4681 aaaggaacga caagattcca actaattgcc cctgtcatcg ccatagctag agagactgca
     4741 ggattcaaat gagctgggcc catgaatcca gaaatataga cagccattgt tacagcgagt
     4801 ccccaaccaa atgcgatgac aatccagcca gaagcctgtg ctttactttt gtttaaattc
     4861 acagccgcac acacaccatc tccaagcaaa actaggatca gtgtcccaat aaactctccc
     4921 atgatttgag tcatatcact gttcatttat ttctccccct ttagttcccg cagatctgat
     4981 tctgcaatca atgcttctaa acgtttagtc tgttgcgcct tctcctcttc tgtccattca
     5041 aaatatccag caattgcatc gatcacaggt tgcttgatct catcaagctg atctcgttca
     5101 aacaacagat gatttgtacg acgaatgaga taatcgcctg gggccaaaac catttcttct
     5161 tctaaaccat aacgtaaccg agctgactca gcgagactca aaccaggata aggtgccatt
     5221 tcttttgcta attcaaagat acgtcgggca ttagtcccgt aaaaatcagc gatataagta
     5281 gcatcttctt cctctaaacc ggctgctact ccttcttttg ctaattctgt cactgtttct
     5341 tctaatttag ttggatcgaa gtttccacct gaaatctgat attttttaga gtcgatttct
     5401 ttcgtctcta ttccgtattc ttcttttaat aattggcgta tcaatcgcaa agctccttct
     5461 gccatcttac ggtaatcggt gattttccca ccagataaag tcagcaaacc atctggttct
     5521 ctttctaaag aactacctct ggaaatcgta gaaggtgctt tttcatcacg gctgttttcc
     5581 aaatggttca acacgtcttc tacttcagca cgagaaactt tgttttcctt atattcactt
     5641 actgtatcga caactttatt gaagctcttg tctgaaatcg atccattatc tccaccattg
     5701 taatcagaac cagaattacc aatcaacagt ggacgaagtc ctgcccagct tgcttcgata
     5761 tcattcaatg tgatatttgc ttctggatag cgatgattga tcacatccaa tagataatct
     5821 acatcttctt gtgtgacttt cggatccgta aaatctcctt ggtaatccgt atctgtcgta
     5881 ccaaagtaag tcttgttttc tcttggaata gcaaaaacca tccgcccatc ttgctttcct
     5941 gtatcgaagt atgtgggttg cggtacaggc agttttttcg catctacaac taaatggatc
     6001 cctttagttg gacgcatttt aggagagact ggacgcgtaa aattcaagtt tcgtacttta
     6061 tctacccaag gaccactcgt attgatcact aattttgctt tgatttcgat cacttcatct
     6121 gtcagcaggt cacgagcttt gacgccaacg atttgatcgc catcatataa aaagccaacc
     6181 gctttcattt tacttactag ataagcccca tcttcggcag cctttttgat attatcaatc
     6241 actaaacggg catcattgtt gcggaaatct agatagacac ccgcgccttt tagcccttct
     6301 tttttcaaaa acggttctct ttccaatact tcttctggtg tcagcgtata attctcatat
     6361 ttggttcctg tcacatttgc cagtttgtca taaaggtcca ttgctacttt gaccgagaac
     6421 atattgaagg ttgttgctcc ttcgccctca tagattggca aaagcattgg atctggtttt
     6481 gggatgtgtg gggcaatact ttgtacgact gcacgttcac caactgtgtc agctactact
     6541 tccacatcaa atgtcttcag ataacgaata ccgccatgaa caagtttggt cgagcgagag
     6601 gaagtccctt ctgcaaaatc ctgcatttcg atcaatcctg ttttgattcc tgatgctgct
     6661 gactggactg ccacaccggc accagtgatt ccgccaccga tgatcaacag atccaattct
     6721 tcttgctgca ttttttgaat gctatcttgt cttgtcttgt ttgaaaacat atggatttcc
     6781 tccttattca aatgcttggg tagcttttac tgcttttttc caaccgttgt aaagtttttc
     6841 tcggcgctct tcctccattt gaacttcaaa tagcttgcca gcttcataaa attcgcggat
     6901 ctcgtctgta tctttccaat aaccgacagc taatcctgct aaaaatgctg cccctagcgc
     6961 cgttgtttcc aagttttctg ctcgttggat cggtacattc aagatatctg tttggaattg
     7021 catcagatat tcattgtttg ctgcaccgcc atctactttc aatacaggaa tcttgattcc
     7081 agtgtcatct tgcatcgtat cgatgatatc tctcacttga taagcaatcg actgtaaagt
     7141 agctttaatg atatcttctt ttgtcgtacc acgtgtcaag ccaaacatag cccctcgtgc
     7201 tgcctgatcc caataaggag cacctagccc aacgaatgca ggaacaacat aaacctcatc
     7261 ttcgcttgta gagcgtttcg ctgcgtcttc tgaatcgcta gctttttgca acatttgaag
     7321 tccatctctc agccattgaa tcgaagaacc agccacaaaa atacttcctt ctaaagcata
     7381 gtaaaccttt ccatttatgc cgtaaccaat tgtcgtcaat aaattatttt tggataattg
     7441 cggtttttca ccggtattca tgacaataaa tgaacctgta ccataagtat tcttgaccat
     7501 gcctggttcg aatgccattt gtccgaataa tgcggcttgc tggtctcctg ccatccctga
     7561 aattggtgct tcgcttccat aaaaatgata accttgcgta taaccataaa cttctgagtt
     7621 agaagtgact tttggtaaca tcactcgagg aatatttagt aaatctaaaa tctcctgatc
     7681 ccaatctagc tgatggatat tgaacaacat cgtccgactg gcattcgagt aatcagtaac
     7741 atgtgcttgt ccatcggtca gtttccatac taaccaagaa tcgatcgttc caaacatcaa
     7801 ttcgcctttt tcagctcgtt cttgcgcgcc ttctacatga tcaagaatcc agcgtatctt
     7861 cgttgctgag aaataggcat cgatcactaa ccctgttttt tcgtggaaaa attcttgata
     7921 gccttcattt tttaagttat ctgcaattcc agctgactgt ctggattgcc aaacaactgc
     7981 atgatagatt ggacgtcctg tatctttttc ccatattact gttgtttcac gctggttcgt
     8041 tatgccaatc ccagcaatct gatttggttt gatccctgat tcgatgaacg ctccggcaat
     8101 aactgactgt acagaattcc agatttcatt tgcatcatgt tccacccaac tttcttgcgg
     8161 gaaatattgc gtgaactctt tttgagaact gccgatgtgt cttcctttct tgtcaaagat
     8221 aattgctcgt gagctagttg taccttggtc aatcgccatg atatacgttg attctgtcat
     8281 tgtgattccc tccattatat gcaaacgctt tctttaacta ctcttatcat actactgttt
     8341 taaaaaaaga aactgtcaaa attttattct ttttttcttt ttttgacagt tttttattat
     8401 gaagctattt tttaatcaat tcttttcttt tttcagcgtc tctattcttc ttttcaatcg
     8461 aataatgtcg taaggtgacg caaattctga cagataatat tcacactgtt ttttgtttct
     8521 ctggcaaaat gtgatcacca aatcatattc tttaccttct tcataacttc caatctcaat
     8581 accgccgatt cccctcagtt ctctgatcag atattgttga aaagcaatac gataaatcgg
     8641 cagactttct aaatcgattc caatcactac tggttttgca atataaaaat cgatcatcaa
     8701 aagaacattg gcatactgga tcatcaggta actaagctca ctatcttcag ctcttttcct
     8761 cggcaattgc tgcaccgctg tttcctgtaa tttcgccagt aactgtgtta aggaatgccc
     8821 gagcattttc tgctgctgtt ccaaaagccg atcacgctca tagacttcga tctttccaac
     8881 aaagaaaaac cattcattgt tgatctggga aatttggtag cctactgcct tttcttcttt
     8941 gattttcaaa cgacgaggac gataatgcca taacatcgtt tcccgaatgt aagtatctaa
     9001 aacagcggtt ggtaactttt tgctccgtgt caaatcatac tgataaaata tttcttcgtc
     9061 tactatcgaa aaagaaacaa aaaagctgta aaacatcatt gtttcgtata cattcagttc
     9121 tgctgctgtt cgactaagat gcatcacgat gattggatcg attgcttgat aaagccggtc
     9181 cgactgatag agtaatttct tttctttcaa agttgcgtac gtactttttt cattcaatag
     9241 tctttttttc gtaatcccga gccagcaagc aatctgtgct tctgctgatg ccgtaaacgt
     9301 tgtttctaat acacggtcca atcctagaat gaacggtctt ttttctggtg tattttgcaa
     9361 aaaaagcggt cgctgatctt ccgggatata ttggaatagt tcataataaa aatatcgtat
     9421 ctgtagctct tctccgtata attgaccgtt tttgatttgt aatccaaatt cctctaagag
     9481 ctggttcaat tctttgatct ttcgaaaaac agaagactca ctgatattaa acgctgtcgc
     9541 taatcgaacg atggatactt gctgatgttc taataaatag acgagcatct taaacttcaa
     9601 gctttcctgc aataaaaaca atagaatttt ttccaaactt acatcttcgt ccaacacaag
     9661 cagcgcctga tactcatttc gctgtacttc tacttttttt tccatcattc ggccaatcaa
     9721 ttcaatatct tccaaatatt gatcaaacga ggcttttgac agttggacat gctctctcaa
     9781 accattgata gaggctgttc ccccttgcag aacaaggttt cgcaaaatca ggatctgtaa
     9841 ctgctctttt ctttctaata atgcttcaat cttcataggc agtattcgcc acctttgacc
     9901 acaccatatc tggcacacgg tattctggct ggaaaaatag ctcatgttct tctaactgtt
     9961 caaacgtcca tccttcttct actgcaattg tcagttggtc aagaaggtgg aacatctctt
    10021 ttttgctttg gagctgtgct ccaagaatct ttttttcaga atcaaataga aatttgagtt
    10081 taactttgct ttgatttatt gctgtaagct tgtagtctac gtatcctgtt tcaatttttt
    10141 ggggataaaa aatgctctct tcttctgtca aacctacgct tcctaagaaa tagccaaacc
    10201 actgattgcc gactggacga taggttccct gatcctttgt ctgtgagcca ctaattgtct
    10261 tgctgacatt tcgtgctgta cggacagcgt tatttactag agaaacatac attttttcat
    10321 ttgttggacg aaaagttact tggatcgcat cgccaatcgc ataaacgtct ttttgagacg
    10381 tttgcaagta gtcatctacc cagatcgttc catcatcatt caatgttaat tgttcttccc
    10441 acatctgatt gtcggggcga gaatgattag ccagcaaaac aagatcatag ttctcctttc
    10501 ttttttccgt ctctacaata gcggtttcat tttcggtcaa agacacaact tgttcttcta
    10561 aaaaaagtga aagcccttga tttttcattt cctttactaa cggttctgtc atttcaggat
    10621 ctagataacg aaaaagaatt gactttcggc tttcgtaaag atggatttgt ttttttcttg
    10681 atgccaaccc ttcagctatt tcgattcctg cttgtcctgc accaataatt gcaattttct
    10741 ttgcattcat gatcttttgc tgaagagccg ttacgttttg tatcttttta actgggtgaa
    10801 taagcgttga ttctgcagaa atatttcgaa acttttgatt tgaaccagtc gctagaatca
    10861 aacgatcaaa atccaatttt tttcctcctg ctaaaacgac ttttttttct ttcaatccta
    10921 ttactgtctt ttctgtgtaa acatggattc gatagcggtc agtcagttct tctttcgtga
    10981 tccaagaaag ttgtttttca ctggtaacct ttccttttaa tatcaaattg aacccgctgg
    11041 gaatccatcc taacttcgat tgcttttcaa tcaaatatat ttcacttgtt ggcatcaatt
    11101 ggcgaagata aatagccgca tgaatccctg caaacgaccc gccgacaatt acaatgttca
    11161 tctgcattcc tctttcttat aaggtgttct actgctaact tctcaatcag aaaacacagt
    11221 attcataact cgctttcaac cttatgatac atagcgatgc agcagaatca accctctcca
    11281 tcataccgtg ttctaggtct gcttctcaat aatgtactag tggacctaat tgaagagtat
    11341 catttctgaa gagatgtttc aaaaaatgta ctcttataaa agaaaaaaac tcgcctttta
    11401 cgtacctctg tccaaatagg aaacagaaaa gaacgaaaag acgagtttta gcgtgtgtaa
    11461 tgtaatagtt agaatagaca catcatttat ttttaattct ttaatgcttc aacaactgct
    11521 tcgcctaatt gatgagcaga ttgaggattt tgccctgtaa gaagccgccc atcaatttga
    11581 accacttctg aaaaagctgc tccagactga tagtttgctc ctttttcttt caaagcatct
    11641 tctgctaaga atggtacttc attcgttgta ccatttgctt cctcttcttc attactgaac
    11701 ccagtcactg tacgtccagc taaaatagat tttccagact catctgtaac tggcaacaat
    11761 cctacaacac cgtgacaaac agctgagacc agcccttcat ttcgatagat ttgtgaagcg
    11821 actttcccaa gatctattgc ttctggaaaa tcccacattg ttccgtggcc accagcataa
    11881 taaaccgctg cataatcatt tgcatccaca tcatcaggac gtaaggtatt tcccaaggcc
    11941 cgatttctga aatcttcatc ttcataatag gcccaatcga tttcatccat attcaaaaca
    12001 ctttgcggtt ctaaaggtac atatccgcct ttaggactga caaaatctgc atcgatccca
    12061 ttttttacta atacatcata aaaatgagtc agttcactta accacaaacc tgtaggacgc
    12121 tcgatctttt cgtacttttc tacatttgtt aatacgatca atactttcat tttcccatcc
    12181 tctttctata aaaattgcgt tattgataac gtgctttcaa gaaattatat aatgggcttg
    12241 gcaaataaag ctctttatat gcttcttcaa aaggtttcgt tacaaattca tcctcacggt
    12301 tttccaataa tttctctgcc cagtcaggat cgacgatcat ttgctgtcca attgctgcaa
    12361 gttctgcata tttcaatacc tgctctacat cttcgcgatt gcggatccca ccgacaccaa
    12421 ctaaagggat ctttccttga atcgtgttat ggaccacttc aagaatcgat ttatcgttgt
    12481 atttatcaga acgagctatt cgatcataca tatttaatga aacatgcaga taatctagtt
    12541 ttgactcacg taatcgttca agaagccaga tcgtgtcttc aaaacggata cctggtgttt
    12601 catattcttc tggtgagaaa cgatacccta cgatgaatgg ttttttagca tattgttcaa
    12661 ccgctgagaa aaccgttgtt aataattctt caataaaacg ataacgattt tccaagctac
    12721 cgccccattg atcctctcgt ctattcgaat gcggagagaa aaattgttgg atcaaatacg
    12781 tatttgctcc atgcaactcg attccatcaa atcctgattc aatcgctcta cgtgtggctt
    12841 tgccaaatgc ttggattgtt tcaacgattt cttcttcact cattgctcgt ggtgtttccg
    12901 catcaggacg ttttgctgca actgcacttg cagatacagg ctgttctcca gataaagttg
    12961 ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat cgcttttgcg ccttctcctt
    13021 ggattgcttt cgctagctca cttaatcgag gaatcatttc atcatgagcg atgctgagtt
    13081 cacctggcca gccttttccg atatcttgga catttgcagc accagtaata acagcaccaa
    13141 gacctttaga acgttgttta taataagtga tctcatcttc ggtgatcact ccattgaaaa
    13201 agctgagttg tgtcgtcata ggcgacagca tcaatcggtt cttcaattga actcctgaag
    13261 gtaaagtaat tgattgatca aattgtgaca ttgtcattcc tcccataatt cgctctgatt
    13321 aacctatcga gaatcatcta tcattaaacc actcgattag ttgtacatgc aactaatttg
    13381 cttaaaaaat tttatttttt ttgtaactta tctgttgcat cattcattgt ctgggctaat
    13441 tctttacttg cttcatttcc caaaactgct ttgtatgact gatgaagttc ttcccagcta
    13501 ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa tgacaagcga
    13561 ccttcgctaa ctgttttgac gtatccttta tattctaatt tttcaacaaa acgagcaatc
    13621 gttgatgggg cggaatagag catttgtgca atatcttttt gtggtattcc atctttttct
    13681 tttaatatca ataaaataaa accataagtt ggtgccagac ctgtccttct aaacgcttca
    13741 tcagctaatt tattcaacat gcgatctagc tttttcacgc taaagaaaag acattcatct
    13801 aacgcttttt caattggctt ttccatttca tcccctccat tggcgtacat atcatagtat
    13861 gatttttatt ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc tagatttgaa
    13921 aaaaagaagt agccatcttc aaagaatttc ggcttgtttt cgacaaaact ccttctggaa
    13981 caccgtgaat cagaggttgt gaaaaagtcg tttagttttg agtatgaaga aaaattttgt
    14041 gaaaatggct ttccacattt ttgcataaat ttggcttaat accgaaaaac tggcttttga
    14101 acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata agaacagcca
    14161 aacaaaaata gttcctcata ttttgtcgtt ttttgagcta atgccgatgg tcagctgatt
    14221 gtacaccgtt tattgagagg tcgtgagcct gccgttgaac tccgagtcac aaggagcaat
    14281 ttcaaaaaac agcttctcat gttttatgac tatggtgact tgtgatcgag gagttggcag
    14341 gagaatactg tttattcgta aagaaggggc tgtgacaaga cttttgtcac agtcccttat
    14401 ttccttttat tataggattt cgccatttga ttggatgact tgtctgtacc aatcaaatga
    14461 ttttttcttt aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa catagatata
    14521 tccataacgt tttgacattt gtgaagtaga agcgctgacg atatcaatgc agccccacga
    14581 cgtataacca aataaatcta ctccatcttt gatagcttcc ttcatctgct caatatgctg
    14641 tttcaaatag tcaatgcggt aatcgtcttg aacggttcca tcttcttcta ctttgtctat
    14701 agctccgagt ccgttttcag ctatgaacag tggtttttga tacctatcat ataattgatt
    14761 caaagcaatt ctcaaaccct taggatcaat ctgccagccc cattcacttg atggaagata
    14821 cggattttta attcctccag ttattaagtt tccttggacc ccttcccaat tttctggatg
    14881 agcactgact gcactagaca tgtagtagct aaaagaaaca aaatcaacag ggtaggctgc
    14941 taagatttct tcatcaccta cttctatttt taagttgaca cctttttctt caaacattcg
    15001 ttgcgtatac gctggataat atcctcttgc ttgaacatca ctaaagaaca aagaagttct
    15061 ttgctgctca attgtttttt gaatatcatc cggatcacaa gaatacggaa caagaagctg
    15121 atagctgatc atacaaccga cttgtgaacc tttgataatt tcatgacaag cttttgtagc
    15181 taaagcactt gctataaact gatggtgtgc accttggtat ttcgcttgtt ccaaatgggg
    15241 atggttttct tcaattactc ctacgctaac aaaagggtga tgttttacac agttgatctc
    15301 attgaaagtc atccaatatt ttactttatt tttatagcgt ttgaatactg tttcagcgaa
    15361 acgaacgtaa aactgaataa gctttcgatt aggccatcca ccatattctg taaccaaata
    15421 taacggcatt tcataatggg aaagagaaac gactggctca atcccatatt ttagacattc
    15481 gtcaaacact tgatcataaa aaaataatcc cgcttcattg ggtgtttctt catcaccatg
    15541 aggaaaaatt cttgtccatg cgatagacat ccgatagcat ttaaaaccca tttcagcaaa
    15601 taaacggatg tcttctttaa agtgatgata gaaatcattt ccgtgtcttt taggataata
    15661 atacttgctg ttgggatcct gcgccgcttt tacttgctga tgcgtcatca tatgccattg
    15721 atctgtccat ttttctttag gtaaatcgct gtcgaatgta tacacatcag acacagataa
    15781 tcctttttca tctacatccc atgcaccttc acattgattg gccgctgtcg ctccgcccca
    15841 taaaaatcct tctggaaatt ttttcatatc gatcccccta aacttgctag ttttcttgtt
    15901 tgattatttt tcttatgact agtaatgata tttttgaatc tactactatt tgtaacaata
    15961 accattgtgg tcggatcata tccctctttg attaattctt gcaaatctac ttccatcaat
    16021 aaatcgcctt tacgtacttt ttttccatca gatactgcac tttgaaagta tttgccattt
    16081 aaattcactg tatcaatacc gatatggatc agtatctctg ctccttcttc actgactacc
    16141 ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc cgtttacagg actgtagatt
    16201 tttccgtccg ttggaatgat cgcaaaccct tctcctaaag atccactagc aaatgttgga
    16261 tcgtttactt cagataaatc aattatttca cctgctgcag gagtacttaa cactatatcc
    16321 tttgaggatt cttcttcgat ttcatccttg tagccgaaaa gaaaagttag catacaggaa
    16381 ctgatcatcg ctaagaaata actagcaact agccaaacaa atccttcacc aatataaatc
    16441 gggaaagaca aaatacttct tgtagcaact gccaaagcac cggctcctgc ggcaccagct
    16501 actccaccaa atatagctcc acatatacaa gcaatgtaaa atggtcgttt atatttaaga
    16561 gtcacaccat aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa tgcagataaa
    16621 gataaacttt ttactttctt atttttcgtt ttcaaaaata ccccaagtac tgctcctgct
    16681 tgcgcagcta cggcagtcat acatgctgga cctagcgtat cacgaccgta gaaagaaaga
    16741 ttgttggtca ttactggtac aagtccccaa tgtaacccaa aaatcactaa cacttgccaa
    16801 aatccgccga taacagcacc agataaaaca gggctaaagt tataaacagc taaatatcca
    16861 gctgcaatca gctgacttgc ataatctgca attggaccta caactaataa agtaattggg
    16921 accatcacga tgatacaaat gaatggtgtc aataaatttc gaatactttc gtgcaagctt
    16981 ttgcgaatct taggctcaat ataagaaaga acccatacag ctaaaattgc tggaataacc
    17041 gtacttgtat accttgctag aatcacgggc atccctagaa atcgcaaggt tatgctatca
    17101 ctatatgcac tgacaatcgt aggataaacg agagctgctg caataaccat agcgatgaaa
    17161 cgatctgtat tgaacttttt tgcagcagta tatgccaaaa atataggcaa gaaatagaaa
    17221 aatgcatcag ctgctgcatt caaaatgata taagttcctg attcagtagt taataaattc
    17281 aaactcgtac ataaggcgag tactcctttg atcaaaccac ttcctgccaa gacgcctaaa
    17341 gtcggggcaa aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc ttttttctca
    17401 gttcctctct ttccactaga tgcatcatct tctagtttca tttgtttgga taatacgtca
    17461 tacacttcat ttactgcatt tcctactacg acttgaaaca ttcctccagc ttcgactaca
    17521 gtgatcacac cttctagttg ctctaccttc tccttatctg ccttcttatt atcttttagg
    17581 gtaaagcgta accttgttgc acaatggtaa acattgctga cattttcttc gcctccgaca
    17641 aatcggacaa tatctttcgc taattgtgaa tagtccattt ttacttcccc cttgttaatt
    17701 aatgccgtag caatccttct tacaagataa gtatagttct tagaaaacgg tttctccatt
    17761 caattagtac cccttataat agcgaacgaa actgtacaat agtgtgtatg gtttacttag
    17821 ccgagcaaat cactcactgc ttgaaagaaa acaagtatat cgtttctctt atagtcaaag
    17881 gagacaaaaa aaacacgctt gatcttcctt tgctgatgta taagtggtat ctcaagctga
    17941 tactctgtga tttttgtttc tacttggctt ttgataagac agcacaacaa agtctcggca
    18001 tttaacggaa gcataactag gtcagaacta ggagagatta gttgctgaat ggaatctatc
    18061 tttttatgga tttttatttg attttcttct ttgtagtaat gatcaagcag ttttgttttc
    18121 tttttttctt caactatggt cagcttttca cgcaaaatct gataaatctc ccaagattca
    18181 aactcatcta gaacactagg tagataaatc aaatcatccc actcaaaaat aagctcttgc
    18241 tcagtagtca aaattgcatc atattccgtt attttgtttg attgattttt gaaaagatac
    18301 actggttcta tccggcattc tttgatccac atgttcatac tttgttgaat ctgttttttt
    18361 agtgtattca aaatactaat tggctggtta ctaacaatca gtatctcccc ttgttttcga
    18421 aaaggattca acgcttcggc aagatacaaa acaaaataag cgctttcagc taaattggga
    18481 cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat aaatttctaa aggataacgg
    18541 gagatgatct tttgtacaaa atgattcatg gtattatgtc cggcatccat cctcgttttc
    18601 atttgtttga tatgggttag caaaaattct gtttcatgga acaataacga aacaggaaga
    18661 tctaaaaaac gaataatcgt ttgctcaaat gctcttagat tcttctctat ttctttgttt
    18721 ttttcatcat taatcatcaa ataatttaac tctaatagtc gattttcaat tgcttgtttt
    18781 tcttgattgg aaaaagaata gggtagttct gatgataact tttttgtgag ggtctctacc
    18841 atcggaataa ataatgtata cctgtttgtt ttttctaaaa gttcttctct tcgaagaatg
    18901 gtcaaaacga gataccgaga aaatcgtatg aagtcctctc ctgaaacaat ataattatat
    18961 gatataagaa cctcttttaa tgtcttttgg acagtcggca tgatattgga aaaaattgta
    19021 ctgatttgtt gaggcaacct tgcgtgttgg atcaccgttt ctgttccaac aagagataag
    19081 aaaattcttt ttgaattttc ttcagcaatg atttttagtc cttttgtcga cgaaacttcc
    19141 agttctaatt taggattgcg ctcgatccaa cgcaaaatcg tttttatttg taatgatacg
    19201 gctgtttttg aaagaaagaa aatgtctgct atcttttgca tactgacata ccctttttga
    19261 aacaataagt acgtcacaat agttgaagct cggaaatcca tgctggaata gattttatgt
    19321 ttcgtcacct cttgtatgat ttgctgttta gtttcagatt caattgattc taaaaaataa
    19381 cctgaacgtt tatttgactg gataatgatt ttcttctcgt agatagcaag catatttttg
    19441 atttcagttt ggatcgtttt cttgttggta cctaaagctt cagctaattc ttcgcttgtt
    19501 atccaatgat tcttatttct taatactaca agaatttgga gttgtcgttt atttaagtcc
    19561 atttgcttac acctcctctt tgttataaac atacaggaaa aacctctcaa
//
LOCUS       DACDHJ010000032        19187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDHJ010000032 DACDHJ010000000
VERSION     DACDHJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.033"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..734)
                     /locus_tag="IV223_000911"
     CDS             complement(<1..734)
                     /locus_tag="IV223_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8711215.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMN"
     gene            905..1273
                     /locus_tag="IV223_000912"
     CDS             905..1273
                     /locus_tag="IV223_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8711216.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1443..1874
                     /gene="mraZ"
                     /locus_tag="IV223_000913"
     CDS             1443..1874
                     /gene="mraZ"
                     /locus_tag="IV223_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8711217.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1895..2854
                     /gene="rsmH"
                     /locus_tag="IV223_000914"
     CDS             1895..2854
                     /gene="rsmH"
                     /locus_tag="IV223_000914"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8711218.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2859..3260
                     /gene="ftsL"
                     /locus_tag="IV223_000915"
     CDS             2859..3260
                     /gene="ftsL"
                     /locus_tag="IV223_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8711219.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3261..5453
                     /locus_tag="IV223_000916"
     CDS             3261..5453
                     /locus_tag="IV223_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8711220.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5479..6441
                     /locus_tag="IV223_000917"
     CDS             5479..6441
                     /locus_tag="IV223_000917"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8711221.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6455..7816
                     /locus_tag="IV223_000918"
     CDS             6455..7816
                     /locus_tag="IV223_000918"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8711222.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7842..8930
                     /gene="murG"
                     /locus_tag="IV223_000919"
     CDS             7842..8930
                     /gene="murG"
                     /locus_tag="IV223_000919"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8711223.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8945..10165
                     /locus_tag="IV223_000920"
     CDS             8945..10165
                     /locus_tag="IV223_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8711224.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10321..11646
                     /gene="ftsA"
                     /locus_tag="IV223_000921"
     CDS             10321..11646
                     /gene="ftsA"
                     /locus_tag="IV223_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8711225.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11669..12910
                     /gene="ftsZ"
                     /locus_tag="IV223_000922"
     CDS             11669..12910
                     /gene="ftsZ"
                     /locus_tag="IV223_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8711226.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12927..13604
                     /locus_tag="IV223_000923"
     CDS             12927..13604
                     /locus_tag="IV223_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8711227.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13618..14217
                     /locus_tag="IV223_000924"
     CDS             13618..14217
                     /locus_tag="IV223_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8711228.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14232..14519
                     /locus_tag="IV223_000925"
     CDS             14232..14519
                     /locus_tag="IV223_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8711229.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14568..15350
                     /locus_tag="IV223_000926"
     CDS             14568..15350
                     /locus_tag="IV223_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8711230.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15371..16078
                     /locus_tag="IV223_000927"
     CDS             15371..16078
                     /locus_tag="IV223_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8711231.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16340..19138
                     /gene="ileS"
                     /locus_tag="IV223_000928"
     CDS             16340..19138
                     /gene="ileS"
                     /locus_tag="IV223_000928"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8711232.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
BASE COUNT         6587 a         3224 c         4161 g         5215 t
ORIGIN      
        1 tgattcattt gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt
       61 tccaccaata catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt
      121 tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg
      181 cttttttgga tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg
      241 gcttccaaag tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg
      301 acctgccaaa tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag
      361 gtctggctaa agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc
      421 tttttgggcg tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga
      481 cttgttttag caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga
      541 ttgaattctt ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac
      601 ggaatttcaa attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa
      661 taccagatcg tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta
      721 aaatatttga tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa
      781 ttggaataca tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa
      841 tgaaacagtt gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac
      901 gttcatgggc acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt
      961 gtttggttgc caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc
     1021 ccctgatttg gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg
     1081 gacaatttct atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc
     1141 tgttttccaa gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg
     1201 gcgtttggtt tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact
     1261 ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc
     1321 ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg
     1381 tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt
     1441 caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct
     1501 caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc
     1561 ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt
     1621 tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg
     1681 aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga
     1741 caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat
     1801 ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg
     1861 atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac
     1921 agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga
     1981 ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg
     2041 gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc
     2101 ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga
     2161 acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc
     2221 tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat
     2281 gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca
     2341 tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg
     2401 tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat
     2461 cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat
     2521 ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga
     2581 acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt
     2641 ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc
     2701 gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc
     2761 aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg
     2821 tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc
     2881 aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt
     2941 cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt
     3001 ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt
     3061 taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa
     3121 cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt
     3181 ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca
     3241 atttaaggaa agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga
     3301 aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta
     3361 ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg
     3421 ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag
     3481 caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt
     3541 attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg
     3601 aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa
     3661 aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg
     3721 gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa
     3781 aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg
     3841 tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg
     3901 gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta
     3961 tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag
     4021 ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa
     4081 cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga
     4141 aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa
     4201 tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg
     4261 aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca
     4321 acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc
     4381 atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg
     4441 tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac
     4501 tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta
     4561 cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt
     4621 tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat
     4681 atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc
     4741 tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg
     4801 tcgaaagcga aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat
     4861 cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata
     4921 cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt
     4981 atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc
     5041 cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa
     5101 aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg
     5161 atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac
     5221 aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg
     5281 gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga
     5341 aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg
     5401 ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga
     5461 aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt
     5521 gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt
     5581 caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg
     5641 ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa
     5701 aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc
     5761 ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag
     5821 cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa
     5881 ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt
     5941 attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc
     6001 gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg
     6061 catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt
     6121 ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg
     6181 ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg
     6241 attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag
     6301 cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga
     6361 tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc
     6421 acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa
     6481 taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca
     6541 tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc
     6601 acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt
     6661 ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt
     6721 agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt
     6781 agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat
     6841 gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat
     6901 tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga
     6961 attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac
     7021 caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa
     7081 atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc
     7141 agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga
     7201 agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat
     7261 aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc
     7321 tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt
     7381 tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta
     7441 taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac
     7501 gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat
     7561 tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat
     7621 ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc
     7681 agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa
     7741 tgccagctgg gatcaatacc caaattttga aactagagga aacagattca tggaagctgt
     7801 gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag
     7861 gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc
     7921 aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg
     7981 tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt
     8041 ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa
     8101 tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg
     8161 tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac
     8221 caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag
     8281 atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag
     8341 aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga
     8401 aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag
     8461 tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat
     8521 attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt
     8581 atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg
     8641 caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat
     8701 atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa
     8761 aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca
     8821 tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg
     8881 atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt
     8941 cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa
     9001 tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca
     9061 agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa
     9121 aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga
     9181 agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc
     9241 caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt
     9301 attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc
     9361 agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt
     9421 gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct
     9481 taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt
     9541 cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta
     9601 cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct
     9661 ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa
     9721 tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag
     9781 caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa
     9841 taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg
     9901 tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga
     9961 agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga
    10021 atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg
    10081 atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac
    10141 tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta
    10201 aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat aagtatattt
    10261 taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc
    10321 atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc
    10381 gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa
    10441 gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca
    10501 gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca
    10561 gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag
    10621 gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct
    10681 cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga
    10741 atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca
    10801 ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc
    10861 aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa
    10921 gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat
    10981 gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat
    11041 atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg
    11101 gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag
    11161 tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa
    11221 cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt
    11281 ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa
    11341 atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta
    11401 tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg
    11461 gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa
    11521 acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa
    11581 ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt
    11641 gattaattca tcctgggagg aataagatat ggaattctca attgataata atatcaatga
    11701 cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat
    11761 gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt
    11821 aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg
    11881 tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg
    11941 cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg
    12001 tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg
    12061 tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg
    12121 tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt
    12181 agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt
    12241 acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga
    12301 ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt
    12361 ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct
    12421 tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat
    12481 gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt
    12541 aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt
    12601 catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca
    12661 agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac
    12721 ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg
    12781 tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa
    12841 ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg
    12901 taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag
    12961 aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg
    13021 taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt
    13081 gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg
    13141 aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg
    13201 aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt
    13261 tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg
    13321 gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca
    13381 aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag
    13441 tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac
    13501 catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg
    13561 caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg
    13621 ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac
    13681 gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca
    13741 acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg
    13801 gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt
    13861 gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct
    13921 acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct
    13981 gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat
    14041 ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca
    14101 ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg
    14161 gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag
    14221 gaggaactac gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt
    14281 tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat
    14341 cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc
    14401 tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact
    14461 tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat
    14521 tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg
    14581 tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg
    14641 aacaggttca aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct
    14701 atattttaga aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat
    14761 atgaacaggc agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg
    14821 aagattttga aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc
    14881 atgggaaaat ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata
    14941 tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg
    15001 ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag
    15061 aaatgattat gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac
    15121 tagacagtgt gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg
    15181 aagctggtaa agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac
    15241 tattagatat tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga
    15301 agacgaaaaa agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg
    15361 tggtttactc atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat
    15421 gcggggatat aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga
    15481 ggaattgact caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact
    15541 tgagtacttc aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac
    15601 ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca
    15661 aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga
    15721 tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc
    15781 gacagaaact gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat
    15841 gcttgagtct caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt
    15901 ctccagctac gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga
    15961 caatgaaaat gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc
    16021 atttgataca caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa
    16081 cagaaaagca ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac
    16141 tgcatggtga aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc
    16201 ggctgacctc gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt
    16261 ggtaccacgg catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa
    16321 taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt
    16381 ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa
    16441 tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga
    16501 tggacctcca tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa
    16561 agacattatc attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg
    16621 ggatacacat ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga
    16681 aatgtccatg gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa
    16741 gcaacgagca gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac
    16801 attagatcct tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg
    16861 ctatatttac aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc
    16921 ggaagctgaa attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt
    16981 caaagacggc aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc
    17041 atggacattg ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt
    17101 ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga
    17161 aatcggttgg gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat
    17221 gactgcaagt catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac
    17281 tttagacgct ggtactggat tagtccatac tgctccagga catggggaag atgactatat
    17341 cgctggtagc aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac
    17401 agaagaagca cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga
    17461 tctattaaaa gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca
    17521 cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat
    17581 cgataaattc cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg
    17641 gggtaaaaca cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca
    17701 gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac
    17761 accagaaaca atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt
    17821 tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg
    17881 cgaatttaca aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga
    17941 agctgtttta cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc
    18001 agaccaatat cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt
    18061 agcaccatac aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat
    18121 gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga
    18181 tatccttcgc ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga
    18241 tatcttaagt caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt
    18301 agcgaataca gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc
    18361 tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg
    18421 gtacgacaaa tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga
    18481 tctatcgtca ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga
    18541 ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt
    18601 attgactcct atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga
    18661 ggagtacgtt cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt
    18721 gatggatacc tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga
    18781 agcaagaaac agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa
    18841 acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt
    18901 ttcaccagac tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt
    18961 atttgatgat gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg
    19021 ccaagttcgt aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc
    19081 aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa
    19141 gggaaaaggg ggtgtgacat gttctttgtc acaccccctt ttgttat
//
LOCUS       DACDHJ010000033        18445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDHJ010000033 DACDHJ010000000
VERSION     DACDHJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.034"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..820)
                     /locus_tag="IV223_000929"
     CDS             complement(<1..820)
                     /locus_tag="IV223_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8711233.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKN"
     gene            complement(793..1473)
                     /locus_tag="IV223_000930"
     CDS             complement(793..1473)
                     /locus_tag="IV223_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8711234.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1496..2434)
                     /locus_tag="IV223_000931"
     CDS             complement(1496..2434)
                     /locus_tag="IV223_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8711235.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2679..3530)
                     /locus_tag="IV223_000932"
     CDS             complement(2679..3530)
                     /locus_tag="IV223_000932"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8711236.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3684..6257)
                     /gene="mprF"
                     /locus_tag="IV223_000933"
     CDS             complement(3684..6257)
                     /gene="mprF"
                     /locus_tag="IV223_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8711237.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6398..7204
                     /gene="proC"
                     /locus_tag="IV223_000934"
     CDS             6398..7204
                     /gene="proC"
                     /locus_tag="IV223_000934"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8711238.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7283..8839)
                     /gene="rny"
                     /locus_tag="IV223_000935"
     CDS             complement(7283..8839)
                     /gene="rny"
                     /locus_tag="IV223_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8711239.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9250..10299)
                     /gene="recA"
                     /locus_tag="IV223_000936"
     CDS             complement(9250..10299)
                     /gene="recA"
                     /locus_tag="IV223_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8711240.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10408..11649)
                     /locus_tag="IV223_000937"
     CDS             complement(10408..11649)
                     /locus_tag="IV223_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8711241.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11889..12467)
                     /gene="pgsA"
                     /locus_tag="IV223_000938"
     CDS             complement(11889..12467)
                     /gene="pgsA"
                     /locus_tag="IV223_000938"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8711242.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12676..13560)
                     /locus_tag="IV223_000939"
     CDS             complement(12676..13560)
                     /locus_tag="IV223_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8711243.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(14338..15624)
                     /locus_tag="IV223_000940"
     CDS             complement(14338..15624)
                     /locus_tag="IV223_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8711244.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15617..16885)
                     /locus_tag="IV223_000941"
     CDS             complement(15617..16885)
                     /locus_tag="IV223_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8711245.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17137..17598
                     /gene="mscL"
                     /locus_tag="IV223_000942"
     CDS             17137..17598
                     /gene="mscL"
                     /locus_tag="IV223_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8711246.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17657..18103)
                     /locus_tag="IV223_000943"
     CDS             complement(17657..18103)
                     /locus_tag="IV223_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8711247.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18196..18441)
                     /locus_tag="IV223_000944"
     CDS             complement(18196..18441)
                     /locus_tag="IV223_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711248.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5263 a         4035 c         3229 g         5918 t
ORIGIN      
        1 tattttttct tctgaaggca accaatattc gtttgatgat atttacaatc aggaacaaag
       61 caataaagac taatgtgatt gttgctcctg gaggtgtatc tagataaaac gagctagtta
      121 gtcctgacag catccctaga aatcctacac aaacactgat agcaatcacg acattgaagc
      181 ttttgcctaa tcgcataccg attgctgcgg gaagaaccat gattgcggat atcaaaagtg
      241 caccggcaat cgggatcatc acagcaatag ccacaccagt aatcacattg aacatcatcg
      301 acatcaaatg aacgggtagc ccatccacgt gggcggtatc ttcatcaaaa gtcagaacat
      361 acatcggccg tttgaataaa aagaacaata taccgatggc aacgaataag attcctaaaa
      421 agatgacttg ctcccatgta atggtcacga tcgaaccaaa cagataggat tggatacttg
      481 ttgctgaatt ccctccgctc aaattcatca atactaacgc tagcgccaaa ccaccagcca
      541 ttaaaatggc aatggagatt tctgaatagg ttcggtagat cgttcgcaga tactccaata
      601 tcacagctgc taatacaacg actagcatgg tcgtaagtgt aggattaagg ttcaaaaaga
      661 atcctaacgc tacccctgct aaagagacat gagaaagcgt atcagccatc agcgattgtc
      721 ttctgatcac taaaaacaca cctagcatcg gggcgattcc ggcaatgaag atagcggcaa
      781 gaaacgctct tctcatgaat tcatatgaaa gcaacgccat tgagaatcct cctttctcac
      841 cagtcggatc tgtctgtccg catactgttt gatatcttca tgatcatggg tgatcattag
      901 aactgatttc ccatggacat gcgcactatg tcggagaaga cgataaaatt catttctgga
      961 ttcttcatcc atacctgttg ttggttcatc aagaacaaat aagtcagggt cagtagcaaa
     1021 aatacgcgct aaactgattc gctgcttttg tccgccagac aattcaccta ttcgccgatt
     1081 tcgcatatcc cacatcccta cagcttctaa agcacgctgt acatgttgat gatctttttc
     1141 agagagacgc ttgaaccagc ggcctcttgg atatcttcct gaacggacta attcgatcac
     1201 cgtactagga aaacctgcat tgaaagatgc aacttgttga ggaatataac cgatacttaa
     1261 tttttttcct tcttgatttt tctcagcaat ctttatcatt ccttttgaag gtttcagtaa
     1321 tcctaacgat gctttcacca aggttgactt ggctgctcca ttttctccag ttaaaatgac
     1381 aaattcaccc gcatctacat gataagagac atcttctagt acaggttcat catcatagta
     1441 aaaggtcaaa tcagatattt cgatataacg caccttacaa cctccattct aaaacttaat
     1501 tgatactttc ttttaaagct ttcagatttt tttccatgac agaaatataa ttttcgcctg
     1561 ctttgatttg ttcattagtc aagctttcta aaggattcaa cacttctagc ttcactcctg
     1621 tttcattcga caatgtctca gctaccttcg aagaggcatt ttcttcaaaa taaatcactt
     1681 tgatgttgtt tttcttcaca aattctttta gctcagctaa gcgacttggt gtgggttcct
     1741 gatctggtga aacgcccgaa atcgctactt gttttaagtc gtattgattc gctaaataag
     1801 caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc atcttttagc tcatcggagt
     1861 atttttgatc cagtgcttca agctttttga tataagcatc ggcattcttc gtaaaagttt
     1921 cctttttatc tggatatttt tcgcctaatt cttcagcaat cgttctgact tcttttatag
     1981 ctaataccgg atcgagccaa acatgtggat cgtattcatg tgaatgacca tgcccttctt
     2041 ctgattcttc atgttcgtct gcttcttgcg attctaaacg gtcgattcct ttagctgctt
     2101 cgatcatcaa ggtctgtttg ctatccactg aatcctttaa cgaatccaca aaaaattcca
     2161 tgtctgaact attatacacg aaaacatctg catcggaaat ttctgctctt tcttttgcac
     2221 ttggttcgaa atcatgcggc tccgttccag cagggatcaa aagtttcacg tttccttcat
     2281 cccctacgat ctcttttgta aaatcataca tcggataaaa agtcgtaacg atcgtcaaat
     2341 cctctttttt gtctgcttcc ttgttcgtat ttccacatgc tgcaaacagt aaagcaccta
     2401 tcattaccgt agctgtcaaa agatattttt tcatttatat gctcctttca atgaacaaat
     2461 cggaatttta cgatttatta acgtgaacta atgtatcaaa ataaaaaaac acagtcaata
     2521 gaaatccatc aatttctgat ttaagcatgt atattcttga ttaaatcgta aaatggatga
     2581 gttgctgctc atatctagcc atttcactac aataaaaaac tgaagaatac cattttagga
     2641 ttcctcagtt tttcatcacc tattttactt cttatatttt attgcagggt tcgtactaaa
     2701 taaacttcct cacaaaaacc ttttaaccca ttatatactc tctgcgctct tgaataatgt
     2761 tgacacaagg caaaaacggt tggtccgctt ccactcatca atgcagcatc tgctccgtac
     2821 ttgatcatac gctctttgat ttgatggatg actggatgcc gctttcccgt tatatcttct
     2881 aaactgtttc ccattttttt gatcatctgc tgataatctt gctctcggat ggcttcagca
     2941 agtccgtgga tgtcttgatg ttggatggct tgcgtatcta cttctctaaa cactgtacgt
     3001 gtagagacac tcattcttgg tttgaccaac acaacccagc actgtggcat agaaggtaat
     3061 acttggattt cttccccttt gccaccgaca aatgccgtac ttccgtagac acagtaagga
     3121 acatctgtac cgacttccaa accgatttct gccagttctt ctaacgaaca gcccagattc
     3181 cataattggt tgatccctct tagagcagct gcacagtctg tacttcctcc gcctaatcca
     3241 gctgctactg gaatattctt cttaacaaag atcttgacac cggttttgat attgtattta
     3301 ttttttagca ataaagcagc ttgataaaca ttgttccgtt catctaccgg taaaaatgct
     3361 ttatttgttt ctacaatgat tttgtcttct gttatttctt caaaaaatag atgatctgcc
     3421 aagtctacgc tcgacatcac catttccaac tcatgatacc catcttgccg tctatataat
     3481 acatccagtc ctaaatttat cttggctggc gctttttcaa tcaattccat cctctcaccc
     3541 attcgaaaag tctaagtcgc aagtattcta tcatagggaa aaataaaagt atagcttatg
     3601 tctgcctagt ggttgatttt gtcatcgttt caacagcttt cacagaattt tctcttcata
     3661 atccaaacaa gttctagtga ttcttataat tctgtgagca cttcttctgc caaactcgga
     3721 acatttcctt tttcttttcc tatcagactg agtagctgca tggtagcaaa tccgaaatta
     3781 cttcctttcc tataagcaaa ataacgtcct tcccaatgat cgacatattt taatttcttt
     3841 tcataagcag actggaagga atagaaaaaa ttaccataat tatatacgat atgcatgact
     3901 ttttcttgaa agaaagaaaa atctgtttct ccaacattag ctaatggagc taagcctaga
     3961 ttggcttccg agtagcccat tttctcgtat acaataaaca gattggctaa cagatagtca
     4021 gctaattcct cagattcttt aggtaagacc cgcaacaaat catatcccgc ctgcttcttc
     4081 gtcatcggct gagcggtgat gaagccaacg actttttgat ctttacggac aataccgaca
     4141 ccgctagata atacatatga tttatcaaat cgtcccacag agaagtattt ttctcgtgca
     4201 ccttgaagcc atttttgtga aacttcattt acagcttgat gtaattcttc cggcagttga
     4261 tcgtaccaag taaaatggta tccttctttc atcaaacgtt gatattcaag ttgctgactc
     4321 agccgagaca agtcttctgt tgctagatca acaatccctg cttcacctac tttcgtgaag
     4381 tgaaaaccca attcatgaag catcactacg tagctttctg atatcttata aaacgcaagc
     4441 tgatagccta aacaatctgc ttgtttcata aactgattcg ttgctgcccg catcatttta
     4501 ggattgccaa ctggatctcc taaaacaaag catttgtttc ctttgatctg ataaccaaaa
     4561 acgacttgat cttggtcgtc ttcttggtaa taaaaataac tgtaacctga caagcgaagc
     4621 tgatggcttg tagctgtgcc tccgtatgtt tccaataaac ggtcaaaacg gtctttttcg
     4681 tactcactgc caagcttcgt agtatcttca gctaaatatt gataaagcgc cactaaagct
     4741 agcaatgaga tgcccaaacc aataagccct gagaaccaca catcatctga aggaaacaaa
     4801 ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc cgctgtgata gcctgcaatt
     4861 gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa agattatagc tccccaagaa
     4921 taaacgaatt ttgttcgata aaattctttt ctggataacc acacagctaa cagaatcaag
     4981 acatatacca atatcaactg ccaagataac gtacgtgtga ttgtatttaa aataccaaaa
     5041 gatagtaaag caatcgtagg cagaaatgcc tttttgactt ttactgaaag tgctctagca
     5101 aggccgagta ataaaaagcc aaccaataga ttcactgtct gatctaaaaa attgaatgag
     5161 aacggcaata atacttgaaa tagccgactg attgcagaca gattagtgat tgttgataga
     5221 agtaccatca tgatccccgc aaaatagaca gcacttacga gcacgatatg cgcaaccttt
     5281 tggaaaaaca gtcgcggaat gttatctaaa aatcgattga ttttgactcc tgtatgtgtg
     5341 atgaacaata gaagtcccgt cataaaaggt agaacataat aaaacaaccg ataatataat
     5401 agccaaacta ctgctacatc ttgccgaaca gacaattgac tcattcctaa gatcatcaaa
     5461 acgtcaaaag tccccattcc acctgggacc atcgtcagca ttcctatcaa tgtagcaatc
     5521 atgaacatag gatagatcga aagcatcgaa atatctattc tcatcaactt gccgatgatc
     5581 aaaaataccg caagggctcc cgtccattgt ccaaaggatg ccagtagcag accaagcaag
     5641 ccttttggat aaaactcgaa gaacaatgtc tttctcttta gataagcaaa aaagaaaatg
     5701 actggtgcta aaaaactacc gccgaaaagc cagatccaat agcttcgaaa aatactttct
     5761 gactgaataa aaaagacatc aataaatgta agaaacgacc atacagacaa accagtcagc
     5821 ataaatagtg cgacttttga tacagtggct aaaaccattt ttctatttgt ctgctttcca
     5881 taaaaactag cccgcagact tgctcctacc acaccaccaa aaccggcgag attgttgatc
     5941 gtgtttgtta cccacgcagc aagaagaaac tcttttcttt ccatttttgg ttttccttgt
     6001 ttttctaaga ctctgatcgt cacccaatca tacaacaaca tcggagtgac acccagcagt
     6061 ccagccagaa tcatgagcag gatcgtcatg cggctttgtt gtcccatcct ttgccaaata
     6121 tcttgccaac tcatgccatt agcaatattt gcgacttgat ttgccacaaa gactaacacg
     6181 gagccgaaaa aaatcagttt taaaatcaga ctatgttcct ttatccattg cagcatcaag
     6241 tatcgtttat ttttcacatt ttcccattcc taacttgtat acttttgtgt cataccccaa
     6301 tgttatctta tttattttaa tttttccact ttagacctgt tctttgcttg cgatgataga
     6361 aaaagaaaga tatcctaatg ctaaaggaga ggataacatg aaagtaggat ttatcggcac
     6421 agggaatatg gcacaagcta ttattgatgg tttattaaaa aaaggaacag tctcgaaaga
     6481 agaggtgttc gtttccagtg gacattttga aaatgcacaa aaatttgctg ctgaaactgg
     6541 cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa tcagatatcg ttcttttagc
     6601 cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta agagaatata gccagaaagt
     6661 cttattcatt tctattgctt caggaaaatc attaatagat ttagaaacag cgcttgaaga
     6721 tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat gtcagcgtag gagcaggcgt
     6781 ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag cttcagcaag ctacgatgat
     6841 ttttgaagca atcggcagcg tctatccttt agcagaatca gatttcagta cattcattgg
     6901 ccttgctggc agctcacccg cattcgttta catgctgatc gatgcgatgg gacggactgg
     6961 tgtactgaat ggtttgtcca aacagacagc cactgagatt gctgcaaaag caattatggg
     7021 ttcttgtcaa aaattccttt caagtgataa aaatccatgg gaacttgtag accaagtttc
     7081 ttctcctggc ggtacaactg ttgcaggtgt agttgcctta gaagaagcag gattcatccc
     7141 agcaatcatc aaaggaatca atacgacgat cgagaaagat cttgagatgt tgaataaaaa
     7201 ataatgatag caatgttttt tcgtgcctta caaaaaaaga agagctgtgg cttttgccgc
     7261 ggctcttctt ttgtattttt tattatttag cataatcagt tgcacgagtt tcccgaataa
     7321 cgattacttt gatgtgtcca ggataatcca gttcttcttc gattcgttta cgaatatcac
     7381 gtactaaacg aacagcgtct aaatccgaga tttcttctgg cttgaccatt acccgaactt
     7441 cgcgtccagc ttgaactgcg taacttgatt ctacaccggc gaagctgttc gaaatatttt
     7501 ccagacttct taaacgacgg atgtagtttt ctaatgattc acttcgcgca ccaggtcttg
     7561 ctgctgagag cgcatctgct gcagctacaa ggacagagat aacagaagta gcttccacat
     7621 ctccatgatg agaagcgatc gcgttgatta ctactgggtt ttctttgtac ttggcagcta
     7681 attccgcacc gatctcaacg tgagatcctt caatctcatg gtcaagtgct ttcccaatat
     7741 catggagcaa cccggcacgt tttgctaatt ggatatcttc gccaagctct gctgctaaga
     7801 taccagctaa ttttgcaacc tcgaccgaat gcttcaagac gttttgtcca taactagttc
     7861 tgaatcgcag acgtccgatg atcttgatta gatctggatg aagtgtatga gcaccgactt
     7921 caaacgctgc ctcttccccg tattcgcgaa tacgttcatc catttcttta cgagatttct
     7981 cgaccatttc ttcgatacgc gctggatgga ttcgtccatc ttgtatcagt ttttccaagg
     8041 tcatccgtgc gatttctctt cggattggat cgaatccgga aagaacaact gcttccggtg
     8101 tatcatcgat gattaaatcg ataccagtca gcgtctcaag cgttcgaata ttacgacctt
     8161 ctcgtccaat gatacgacct ttcatttcat cattcggaag ggtaacgact gaaacagtcg
     8221 tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa tgataataag tttttcgctt
     8281 tgcgatctgc ttcttctttg gcacgctgtt cagattcttt taccattaga gttaactcat
     8341 gattcagctc ttcttcagtc gatttcatga tgacatctcg tgcgtcctct tttgataaac
     8401 cagcaatttt ttctagttct ttttgttgat cttcaatcat tttttcaacg tctttttctc
     8461 gctcttcaat taattgctgt tttgaactaa gtgcttcttc tttactttca agtgagttct
     8521 cgcgtttttc taacgagtca tctttacgat tcagcgtttg ttcgcgttga atcaaacgat
     8581 tctcttgaga ttttaattct aacttacttt ctctcagctc actttcaact tcagaacggt
     8641 atttctgatt ttcttcctta gcttctaaca atgcttcttt ttttagagtt tctgcttctt
     8701 tttttgcact atcgatgatt ccagcagctg atgattgtgc accagctatt tctttctcgt
     8761 gacgtgactt tgcgaccata aaccctaaac caagaccgac aattaaaccg atgatagcga
     8821 ggagaatgtt gaataccata aaatccacct ccatactatc tttttcattt ttatcatcta
     8881 tacagcttct atgataaatt attctaaact cgtcaatatg catcttcgca tatatgtgta
     8941 caaaaaacat gcacaacttt attctaatgt ttgtaccccg tagtgtcaac tttaaataaa
     9001 caaagaaacg gctttctact tattcattaa acgatggttt ttaaaataaa tgacactagt
     9061 ttcatatagg taaaccttta aattgagaaa cttttctatt aaattattct ttgtagatga
     9121 acagatattt ttattttatg tacccactcg tataattaaa taactcacga tttctttatt
     9181 caataaaaaa acggcaggtc aaggagagta cctactcttc agaccagccg tttttgttta
     9241 tcataatttt tattcttcta ctaaaggcaa ttcttcttgt cctttttcat cttctgggac
     9301 agcgacttct tcaccaatac cataagctgc tctaactaga gcagaaactt ctgccatcat
     9361 ttcaggatga ttagccatat agattttagc gttttcccgt ccttgaccaa tccgatcttc
     9421 tttataagaa taccaagcac cgcttttatc aacaatgtct ttttctactg ccatgtctag
     9481 aagttctcct tcttgtgaga tccctaaacc atacatgaca tctacttcag cgattttgaa
     9541 aggaggtgct actttgtttt ttacgacttt gatctttgta cggttaccaa caatatctgt
     9601 accttgtttc aattgttctg cacgacgtac ttccaaacgg atcgttgcgt agaatttcaa
     9661 tgcgcgtcct ccaggagtga tttctggatt cccaaacatc acgcctactt tttcacggat
     9721 ttggttgatg aaaatagcga ttgtttttgt tttgttgatc gaaccagaga gtttacgcaa
     9781 tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag tcacccattt cgccgtcgat
     9841 ttctgctcgt ggaactaaag cagcaactga atcgactact acgatatcta cagccccact
     9901 tgatactaaa gcatcagcga tctctagacc ttgttctcct gtgtcaggct gtgaaagaag
     9961 tagttcatcg atattcacac ctaatttttg tgcatattgc ggatctaacg catgctcagc
    10021 atcaatgaaa gcggccgttc cgccattttt ttgtacttct gcaatagcgt gtagtgcaac
    10081 tgttgtttta cctgaacttt caggtccata tacttcgatg atacgtccac gggggtaacc
    10141 gccgacacct aaagcgacat ctaacgcaag agagccactt ggaattgtag agatttgttg
    10201 gtcgattttt tcgcccaatt tcataataga gcctttacca tagctctttt caattttttt
    10261 cagtgcagca tctaaggctg ctttacgatc atctgccaat acgataatcc tccttatatt
    10321 tacaaatcag agcaacattt cattatcctt atcatatctt ttttagtgaa aaaaagcaag
    10381 aaaaaaccga acaaatattc gcatttatca ttttcttaaa agctgttttc tcaaaagatc
    10441 aaatccttgc ataaccgcat tatatctgat agcagcacga tctctggcaa agtgatattt
    10501 ttccgaaacc acgccgtctt tagatgaaat agcgatccat actgttcctg cagattgacc
    10561 ttctaacgtg tccggtccag ccactccggt gaaagctaca ctgtaatctg ttcttgctat
    10621 ctcacgtgct tgaatggcca tctgctccac acattctttg ctgactgttc catattctgc
    10681 taatagctcc ggatcaatct ccagaaaacc agcttttgtt tggagcgagt aggtaacgaa
    10741 tccgcctgga aatacttcag atacaccagc aatgtcgcct aatgcggctt ggaaggcgcc
    10801 ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt tctttcagta gttccactac
    10861 gacttttgct agcgaattat catcgccgta tccataaaaa tattccccta ctcgttcttg
    10921 gattttttct tctaatgcta aaagagcttg attccctgcc tgaacatcat ttgttttcac
    10981 tgtcagtcgt aatgtcactt catttggttt ggcatatggt gcgatcgttg gattgatctg
    11041 tgtctcaatc agatctttta gctcagtgac cagtcgcgat tcaccaatcc cataaaagcg
    11101 caaaacttta gaaattagtt tttcttcaga aggaaaacgt tcctctagta aaggacgtac
    11161 ttgttccaca aacattggtt tcaactcatt aggcggaccg ggaagaagga tatacgcatg
    11221 tgtatctgtt tgataaaaag tccctaaagc aagccctgta cgatttggta gaggaacccc
    11281 gccttcgata atctgggact gttgcagatt gtttttggtc atttttcgat gggttgtttc
    11341 aaaatatgcc aataattttt tgtatccttc cgtattttgt atgagagact taccgaggtg
    11401 agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt cctaatccgc cacataagag
    11461 tatgagttca cttcttgttt cagctagagc aatcaactct tctaaacgct gctgattatc
    11521 tcctacaact gtttgataat aaacttcgat acctagatca gctagctgtt ctgacaagaa
    11581 agtggcattc gtattcacga cttgtcctag taaaagttct gttccgacag ctattatttc
    11641 tgctttcatt acttcccgct cccttatata gtatacgctg tatttttata tttttttatt
    11701 taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca gctccttgtt ccaaataaac
    11761 agtgttctcc tgccaaaacc tcggtcataa gtcacaatat acataaaaca tgaaaagctg
    11821 ttttttgaaa ttatcccttg tgactcagag ttagacggca ggctcacaac ctctccacta
    11881 aacgatactt acattgatcc tttgaacaca ccgctgtttt tgatgaagta atccacacct
    11941 gaatagatag taaagatcag acaagcataa agcatgattt gatctactgg aataccgatc
    12001 aggctgaatg ggatattatt gataaacagc aagataatag caaccatttg tgtagccgtc
    12061 ttgacttttc ccggccaagc tgcagccatg acttctccat gttcgacaag tagtaaacgc
    12121 aagcctgtga cggctaattc acgacagaca atgatcgcta ctacccaacc aggggccttt
    12181 ccttggtcaa ccaacatgat aaaagcagtc ataactagca ttttatctgc caacgggtcc
    12241 gcaaacttgc caaaattagt gacaaggcct cgcgcacggg caatttttcc atctaaccag
    12301 tctgtgatac tagctaaagc aaaaataact gcacctacta gctgtgtgac agccagtgtg
    12361 gtgtctccta cgttaaattg tccccaatct aaaggtaaac ctacaaccaa aatgaaaatc
    12421 gggatcatac aaattctaag tacagttaat ttatttggta aattcacact atcgactcct
    12481 ttcttcccta ttctacgtga ctatcagcga agtttccacc gatattattt acttttccaa
    12541 atgaatttat tcaaaggagt atttttattg aaattgatct ttttgaatta acgaagaagg
    12601 accgcgacac agatttgtca cgatccttct tttttatacc tacaagtaaa cagatgcgta
    12661 aactaattta tacgatcatt ctgcgtattg aagagtaagg ttcacatttt ttggactctg
    12721 attgttttcc cctggattaa ctggtacgga ttctccattc gctttgatcg ttgcattgct
    12781 tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc ggtaacacgg tactctgtgt
    12841 ttcatttgct gccagtgtcc cttgataaac ataggcattg tctactagca cacctaacca
    12901 tacacgattg acagcagtaa attcaagggt tacaggcttg gtagcgtctg tgatatttac
    12961 tgcgatggca ctgccagaat cttctcctgt agctattgcc attttctttt cttcacttga
    13021 ttttgttgtt gattctactg tagaagactc tttggtctgc gtgctttgct ctgaggaaga
    13081 ggactcctcg actgatcctt ctactttaag cgaagaagct gtgtctccga ttattggatc
    13141 agcttgacga tcttgccaag tcatatagcc cacgacaacg acaatcgcca aagcaaccaa
    13201 tcctaataaa atgacaggaa gacttgtcca aaagcgactt ggatgcgatt cttcctgatg
    13261 cattgctttt ctagaagtac ttactgtttc aggctgcgga cgttcaggga ccgatcctgt
    13321 caatttgctt ttcccatcaa aaacatcgac taaatggtct ccatcttctc ctacagcttc
    13381 agcgtattgc cgaataaatg cccgcacgta aaatttacca ggcaattggt caaaatcatc
    13441 attgtcgatt gcttccaaat aacgtttttg aatttttgta atctgctgta attcatctaa
    13501 agatattttt ttattcaggc gtgcttgccg taatttttct ccgatggttt caaaagccac
    13561 tcgttcatct tctccagtct gtttgattct ccgaagcatc tgctgttgca acgaaagacg
    13621 ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc ttttttccga ataaacattg
    13681 ttctcctgcc aactcctcgg tcacaagtca caatatccaa taaatataag aagctgtttt
    13741 ttgaacttgt tccttgtgac tcggagttaa acggcaggct cacgacctct ttcttaaacg
    13801 gtgttcaatc agctgttcct cggtattagc tcaaaaaccg gcaaaatata aggagatatt
    13861 tttccgtatg tttctctaat actcaggtca aaacgctgat ttcacaacct cttccttaaa
    13921 cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cattaagaag
    13981 ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttgg
    14041 tacacggtgt tccagaagta gcttcttcgg caataagccg aaatatcaca aaaatttgag
    14101 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
    14161 ttgattactt cgtttgattt tattttaata taagttgatt aaaagagagc atgatttgtt
    14221 ttattttctc actaattttt ccaacacaac taagaatacc ataaaagctg attttggagg
    14281 aggttcctac tacaagtcta aaacgaaatt taagactttt cctcataaaa tactggttta
    14341 ttttggtctc atgtaaaact cgctgaaggc ctcttcactt ataaatgctt ccccagctgc
    14401 cagtacgtct ttcatttgga tcgaatcgat gatttcagga agatcaaata aagttcgatc
    14461 gccaaataaa ctttgcgtaa attgattggc aatatattcg atcgaattta atgattggaa
    14521 atattgtcca agcattttct tcttcagcaa atcgagattt gtttctgaca cttccggatc
    14581 atcagcgaat ccaagaatga tttctttgac tttttctgct gctttctctg gttcgtccgt
    14641 atctccgctg aagtccgcaa agtgaaattc acgatccaag ctgaattcaa aaccaaagct
    14701 gtcatcgata attccttgat tgtacatcgc tagatagttt cgagaagtat tgcctaaaat
    14761 catttggaac aacagattga ttgctgtttt atagcgtaac agttctgttc cttcttgcgg
    14821 caatgtgtct aatcctttaa taccaagtac aaatttatct cgtgtaatgg ctgcttccaa
    14881 tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa taacgtacaa tttcttgctt
    14941 tggagggaaa ttttttgctt cttggttttc ccgaataagt ttcatcaatt tctcaggctc
    15001 catcttacca actacaaata aaaccatatt gcttggctga taaaacgtcc gatagcacgt
    15061 atacaagtct tgagctgtga ttttgtcgat actttcaacc gtgcccgcaa tatcgatatg
    15121 caatgggtgt gtaggataca agttatttag tatgccaaaa aacatccgcc agttagggtc
    15181 gtcttcatac atttggattt cctgaccgat gattcctttt tccttgttta cagtttcttc
    15241 tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc agattctttt ctacctgatc
    15301 cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg aaagcattgg cggaagctcc
    15361 ttgtttccca aataattgaa agacatcacc atcttctttt tcaaacaact tatgttctaa
    15421 aaaatgggca attccatctg gaactttcac cttttctttt tcaccatagg gaataaattc
    15481 gttatcgatc gaaccataat tcgtactgaa caaaccatat gtcttgtgat agtcattttt
    15541 aggcaacaaa tacacggtca aaccgtttgg cagtacttca tgataaagtg tttcattgat
    15601 ttgctcatat tctgttttat tcatttgcat ttcctcctgc taaaaagaag attgcctgca
    15661 attcgatttg ctcagctact tgttgtatct ctttgatcgt tacgctctcc atacgtttca
    15721 accattcttc atcagataat tttgtttccg gcagccagct gtccaaaaaa gaagcttcga
    15781 ttgccgcttg cggagagtct agagaaagca gatattggtt tctcagcatt gcttttgtct
    15841 gagacagttc caactctgtt atttcaccat ttcgcaaact ttctaattgc tcgtgaatca
    15901 gatgaagtac ttgatttcgg ttcttttcat cgataccggt ttggacactc atgaatcctc
    15961 ggaacgtatc cacgctacta gaggcataat aagctagact ttctttctct ctgacattca
    16021 tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt aaagaccatt agcgcaaaac
    16081 gttctggttc atcgtaatac acatttgtct gataagctag gttcagcttt gcttgaacga
    16141 ttggctcacg tacttgtcgt tccttcacga tattcacttg aggttgggta taaaacagat
    16201 ctggataaac tgtttcccgc gactcaaatg gtaattgccc aactgcttca gctgctttgt
    16261 ccggatcaac atcgccgaca acaaaaatgt cgatttgatc atttgccatc atttcttgat
    16321 agtaggacgc tagcgaacta gcagtgatct tgtccaaatc ttcgactacg cccaaccctg
    16381 ggatcttttg ttccgggctg ttttcaaaat aaagttcttg caaggcatag gaagcaaaag
    16441 tttgtttatc ttccttcaaa ctttccaaat atagacgcag attgttcttt tccaagtcaa
    16501 acgtcaactg atcaaactga tgatttttaa tatttggata aaataaaacc tcttttaaaa
    16561 aatctaccgc ttgagaaaaa aggtttggat cattgacata ttcaccattc acaaaactga
    16621 tccctgcgtt tagccaatgt aaatttcctt tttttctaac tgataatcca aagcttgcgc
    16681 cataaagctc tgcaagttgc gcacttaact ttgtctgatc cgaataattc aaactatttg
    16741 tttccaacat gctgctgaga agtgaacgtt tcgtgatgac ttcttgttgc agacgcgcag
    16801 taaaacggat aaaaatccgg atcgttttgt attgttttgt tggtaagaca tgaagattca
    16861 cgccttctgc caattgatac accatattta tatgctcctt ttcacagttg attgcccgct
    16921 tactctttta atgggtcaac ctgccttttt tacttattcc tctgcttgaa atttataagt
    16981 tcaggcaatt cgccgttttt tctaaatgat gttacacttt atagtatagc atagatgttt
    17041 tttatcaaaa gaaacagacg gctaaaagcg tatattgttt ttttattttt tatttttcga
    17101 tatactaaag acaaataaaa gaagggaggg tcattcatga tcaaagaatt caaagagttc
    17161 atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg ttattggtag tgcgtttaca
    17221 agcatcgtaa accaagtggt gcaaggcttg atcacaccac taataggttg gtttgtcgca
    17281 atgatcacag gtacttctga tttagaagga gcactttcaa ttcttgactg gtcgccgaca
    17341 aaaggcgtca catttgcgtt tggtaacgtt attagtgcta tcattacatt tctgatcact
    17401 ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg cgaagaattt acgctccaaa
    17461 gaagagcaag tggaagtgga agaaactcct actgcagaag attatcttag tgacatacgc
    17521 gatcttcttg ccaaacaagt ggcagaagac aatacacatc cagtagagca aacaaatctt
    17581 tccgatactt ctaaataata aagaatctaa aaaaaagaaa gccgattttc agtgtcggct
    17641 ttcttttttt agattcttaa cttgctactt gttccttttt catgtcgata tgtggaatat
    17701 catcttccaa atacacatct gagattgcct tgaatccaaa cgactcataa aaagcttgta
    17761 ggtattcttg tgcgccgatc acgattggtt tttctggaaa atttttctgg atcatgttaa
    17821 tcgtttcttg aacaatcttt cttcctaaac catctttacg gttgtcctga tgaacaagga
    17881 cacgtccaaa atggatactc tccgcttctt catatactcg cgtgtaggca aggatcttcc
    17941 catcagtatc ttgaaaaaac gtatgccaag cttctccatc aatgtcatca acttcttgat
    18001 aaggacactg ttgttccaca acaaaaactg cgattcgttc tttgacgatc gcgtaaaact
    18061 ctgctgttga caattcttca aatttcttta cctgatattc cactaagcaa acgccccctg
    18121 cttttttatt cttagcatac cgattcgcac acaaaaaagc aaacgaacct tacgaatctc
    18181 aggctcgttt gctttttaaa ctgcttcagt taaaaattcg atctcaactg tccgagtaag
    18241 tacatctgaa tagctgtaag atactcgttc aaaagaattt tcttcaggat ccaaatcaac
    18301 aacgaatact gaaggatagg tttctgttaa aacgccgcgg cgttctgttt gacgctttct
    18361 tcctgtctga gcaaccaaca tgattgggct gccgatacga ccttctaaat ctttcttgat
    18421 tgaagctaaa gttgttggca tacag
//
LOCUS       DACDHJ010000034        18244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDHJ010000034 DACDHJ010000000
VERSION     DACDHJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.035"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IV223_000945"
     CDS             complement(109..564)
                     /locus_tag="IV223_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711249.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IV223_000946"
     CDS             complement(718..1926)
                     /locus_tag="IV223_000946"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8711250.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IV223_000947"
     CDS             complement(2038..2370)
                     /locus_tag="IV223_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8711251.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IV223_000948"
     CDS             2518..3393
                     /locus_tag="IV223_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8711252.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IV223_000949"
     CDS             complement(3475..4056)
                     /locus_tag="IV223_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8711253.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IV223_000950"
     CDS             complement(4062..5321)
                     /locus_tag="IV223_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8711254.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IV223_000951"
     CDS             complement(5323..6369)
                     /locus_tag="IV223_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711255.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IV223_000952"
     CDS             complement(6604..6879)
                     /locus_tag="IV223_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8711256.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IV223_000953"
     CDS             complement(7122..8432)
                     /locus_tag="IV223_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8711257.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV223_000954"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV223_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8711258.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IV223_000955"
     CDS             complement(9978..10658)
                     /locus_tag="IV223_000955"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8711259.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IV223_000956"
     CDS             complement(10707..11342)
                     /locus_tag="IV223_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8711260.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IV223_000957"
     CDS             complement(11408..12811)
                     /locus_tag="IV223_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8711261.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IV223_000958"
     CDS             complement(12798..13829)
                     /locus_tag="IV223_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711262.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IV223_000959"
     CDS             13860..14081
                     /locus_tag="IV223_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8711263.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IV223_000960"
     CDS             complement(14246..14983)
                     /locus_tag="IV223_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8711264.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IV223_000961"
     CDS             complement(15136..16476)
                     /locus_tag="IV223_000961"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8711265.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IV223_000962"
     CDS             complement(16713..18236)
                     /locus_tag="IV223_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8711266.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5140 a         3892 c         3092 g         6120 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttct
//
LOCUS       DACDHJ010000035        18107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDHJ010000035 DACDHJ010000000
VERSION     DACDHJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.036"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(13..1521)
                     /locus_tag="IV223_000963"
     CDS             complement(13..1521)
                     /locus_tag="IV223_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8711267.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1680..1871
                     /locus_tag="IV223_000964"
     CDS             1680..1871
                     /locus_tag="IV223_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711268.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2074..2991
                     /locus_tag="IV223_000965"
     CDS             2074..2991
                     /locus_tag="IV223_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8711269.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2984..4057
                     /locus_tag="IV223_000966"
     CDS             2984..4057
                     /locus_tag="IV223_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711270.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4205..4723
                     /locus_tag="IV223_000967"
     CDS             4205..4723
                     /locus_tag="IV223_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711271.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4704..5096
                     /locus_tag="IV223_000968"
     CDS             4704..5096
                     /locus_tag="IV223_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8711272.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5172..6107
                     /locus_tag="IV223_000969"
     CDS             5172..6107
                     /locus_tag="IV223_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8711273.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6240..7019
                     /locus_tag="IV223_000970"
     CDS             6240..7019
                     /locus_tag="IV223_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8711274.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7012..7329
                     /locus_tag="IV223_000971"
     CDS             7012..7329
                     /locus_tag="IV223_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8711275.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7573..9183
                     /locus_tag="IV223_000972"
     CDS             7573..9183
                     /locus_tag="IV223_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8711276.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9340..10188
                     /locus_tag="IV223_000973"
     CDS             9340..10188
                     /locus_tag="IV223_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8711277.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10419..10898
                     /locus_tag="IV223_000974"
     CDS             10419..10898
                     /locus_tag="IV223_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711278.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10931..13159
                     /locus_tag="IV223_000975"
     CDS             10931..13159
                     /locus_tag="IV223_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8711279.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13288..14931
                     /locus_tag="IV223_000976"
     CDS             13288..14931
                     /locus_tag="IV223_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8711280.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15004..15549)
                     /locus_tag="IV223_000977"
     CDS             complement(15004..15549)
                     /locus_tag="IV223_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8711281.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15572..16057)
                     /locus_tag="IV223_000978"
     CDS             complement(15572..16057)
                     /locus_tag="IV223_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8711282.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16047..16859)
                     /locus_tag="IV223_000979"
     CDS             complement(16047..16859)
                     /locus_tag="IV223_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8711283.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(16998..>18107)
                     /locus_tag="IV223_000980"
     CDS             complement(16998..>18107)
                     /locus_tag="IV223_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8711284.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5879 a         3298 c         3628 g         5302 t
ORIGIN      
        1 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
       61 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
      121 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
      181 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
      241 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
      301 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
      361 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
      421 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
      481 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
      541 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
      601 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
      661 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
      721 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
      781 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
      841 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
      901 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
      961 caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
     1021 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
     1081 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
     1141 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
     1201 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
     1261 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
     1321 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
     1381 aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta aatataattt
     1441 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
     1501 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
     1561 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
     1621 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
     1681 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
     1741 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
     1801 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
     1861 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
     1921 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
     1981 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
     2041 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
     2101 tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca agaatcgtct
     2161 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
     2221 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
     2281 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
     2341 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
     2401 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
     2461 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
     2521 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
     2581 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
     2641 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
     2701 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
     2761 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
     2821 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
     2881 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
     2941 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
     3001 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
     3061 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
     3121 aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
     3181 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
     3241 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
     3301 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
     3361 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
     3421 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
     3481 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
     3541 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
     3601 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
     3661 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
     3721 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
     3781 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
     3841 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
     3901 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
     3961 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
     4021 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
     4081 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
     4141 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
     4201 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
     4261 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
     4321 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
     4381 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
     4441 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
     4501 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
     4561 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
     4621 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
     4681 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
     4741 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
     4801 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
     4861 tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
     4921 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
     4981 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
     5041 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
     5101 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
     5161 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
     5221 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
     5281 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
     5341 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
     5401 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
     5461 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
     5521 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
     5581 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
     5641 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
     5701 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
     5761 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
     5821 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
     5881 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
     5941 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaggcttact
     6001 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
     6061 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
     6121 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
     6181 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
     6241 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
     6301 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
     6361 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
     6421 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
     6481 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
     6541 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
     6601 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
     6661 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
     6721 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
     6781 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
     6841 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
     6901 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
     6961 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
     7021 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
     7081 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
     7141 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
     7201 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
     7261 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
     7321 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
     7381 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
     7441 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
     7501 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
     7561 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
     7621 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
     7681 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
     7741 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
     7801 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
     7861 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
     7921 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
     7981 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
     8041 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
     8101 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
     8161 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
     8221 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
     8281 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
     8341 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
     8401 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
     8461 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
     8521 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
     8581 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
     8641 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
     8701 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
     8761 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
     8821 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
     8881 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
     8941 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
     9001 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
     9061 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
     9121 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
     9181 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
     9241 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
     9301 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
     9361 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
     9421 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
     9481 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
     9541 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
     9601 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
     9661 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
     9721 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
     9781 atcgatcgaa tccgagataa tacctacgct tatctgattg tagatgattt gaacaattta
     9841 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
     9901 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
     9961 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    10021 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    10081 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    10141 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    10201 aaaaaacata ccaaaaatat tccaacatca tcttggactt tctacatgtt actaatgagc
    10261 gtgtaaaaga acgagatggt gtttttattt ctatcaatga agctcttttt ctttgcaatc
    10321 ctcacttcaa tcctctataa tgtcgcatat agtttagtaa atgagtaaac tactaattta
    10381 cttatttact aaaaataaaa ggatgaggag ttaaaataat ggaaatacca acaaatttaa
    10441 aacataaacc agtaatcgtt gtagaagact atgataaagt tgatggaaga aatgcattaa
    10501 atacggatgc aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg
    10561 atatctcagg caaggtatgg cgacatactg gagaaaaatg gtcacgacag tcggaagaat
    10621 taccaatcac acgtatttta gatttagcga tcttagctgc acaaggaagt ttatacttcc
    10681 aagatgctta tcgttatgaa aaattttatg atccagaaaa tccagtcgta gacatcattg
    10741 gattacaagg gaaccgaatg acggtagaag tagataccaa aaatccaaag atcgatgaag
    10801 acatcatttt gtattacgat acactacaaa aagacggaga actgattggg gaacgattcc
    10861 gtattttaaa acgtctttta gatgagctag gatattaata gacagagatg atcaaaaggg
    10921 gaagagaagc atggagaatt tctcactaac agaaaaccga accttttcac ggctagaaaa
    10981 gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc
    11041 ctccgaaatc aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga
    11101 aggagatgcc gggtcgggga agacacaatt agccaaagca ctatctgctg atctgcaact
    11161 tccctataca aaagtgacct gttttgcaga tatggacaaa tcagatgttt tcggagcctt
    11221 attacctgta gtagattcta ataaggaaca agaccaagaa ttactagaag ctatttacca
    11281 aacagataca cttgaagctg tactgacact gatccagaac cattacggca ttgatccttt
    11341 ttctgctaaa gaaaaattag cagagctagt tcaaaggatc gaagctgatt atccagatgc
    11401 aatccattac agctattacc cttctgaaat cgttcgagcg attgaaaaag gatatttatt
    11461 agagattcaa gagccgactg tcatccgaga tgcctctgtt cttgtggcac ttaactcagc
    11521 gctcgaacca aatgggatgt tgaatttacc gacaggaatc attcgtcgcc atccagattg
    11581 cgtcattgtg atcactacaa atcgaaatta ccaaggaaat cgcccattaa atgaatcttt
    11641 aagagatcgg atgcaacatg cggaaaaaat ggatcttcct gccttagaag tcatggcaga
    11701 acgtgcgatg gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga tggcagagat
    11761 tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg
    11821 ttcttatttt tattgggcaa atacctataa gcaaggacaa gatttactcc agtcgattta
    11881 tccaaaagtt ttgtataaat tatccactga ttcagatgaa ttggcaattc ttgaacaagc
    11941 tttgtctgac agtggattgc tactggaact tcaggacata ttgagaaaag aaaaatgggg
    12001 tcatcctcaa tctgagcata caaaaggaag aaccattagc gccgaagaag caaatgaacg
    12061 aaacatcact gaaaatgctg aagaaattct gcgcttagat gaaaagaaat cgttgtctgc
    12121 tgaagaacca aatgagggag aagagaaaca agaagaaaca atagaaaaaa acgagattcc
    12181 tcctcaaatg caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca
    12241 aacaggagat gattctcaag aaatgtctgc ttctgacatg gaagcacatg ataaggaaat
    12301 caagaagcaa ctgaataaag aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga
    12361 aggtctcatc gttcaccgac cagctttcca aggaaaaacg ccggaagcac gtgagctaca
    12421 catgcaagta atgccaatag tagaatcact tagccggcaa attttagagt tactggataa
    12481 tgaacaaaca gagacgtacc aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa
    12541 agtagcatat ggtgatttga gaaattttga caaaaagaac ccgccacatg agcaaccttc
    12601 tttagcggta gctgtacgga tcgatgaatc tgggtcaatg atacgggatg atcggatcga
    12661 agcggcaaaa aaagcagcga ttgccattgc cgaattttct aagaaagtcg atatcccatt
    12721 gttgatttac ggagatacag cagaccgttc gtcaagagaa aagacttctt tgttttctta
    12781 taaagaattt gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag
    12841 gcaaaacaat cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa acagacaatc
    12901 cgcaacaacc aaactgttgc ttaatattag tgacggtcag ccaaaagcgt tgccggatta
    12961 tacaggcaaa aaagcaaaag aagatatcca gtctgttttg aaagaatatg aacgtcaagg
    13021 aatcctattt gtctccgcag ctattggcca agacaaagaa gaaatcaagt cgatctacgg
    13081 tgaaagtcgt tttgtagata ttactgactt gaatgaattt cccaagcaaa tgattcaact
    13141 gattgcccgc tatctatgat aaaataaagc tcaacgaacg cacttagcag attcgttgag
    13201 cttttcgttt ctagttaccg gtaacaagga tacttctaaa atagaatcgg ttataatcaa
    13261 gagggaccgt taaaggagtg aaacgatatg atacaagaac aaaaatggtg gaaagaagca
    13321 gtaggctatc aaatctatcc tgctagtttc aaagatagca atggcgatgg tcaaggagac
    13381 atcaatggga tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg ctttatctgg
    13441 atcaatccga tctatcaatc tccatttgtc gataatggtt atgacgtaag tgactatcaa
    13501 gatatagaag agagattcgg aacgatggaa gattttgatt tattgctaaa agaagcacat
    13561 gaattaggga tcaaagtgat tatggatctt gtcatcaacc atagttcgga tcagcatcaa
    13621 tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc gcgattatta catttgggtc
    13681 gacggggtag atggcaaaga accgaataat tggacatcga ttttcggcgg ttctgcttgg
    13741 gagtattctc atgaaactgg acagtattat ctgcatgttt ttgcaaaaga acaaccagac
    13801 ttaaattggg aaagcgagaa attgaaagaa gaactattca atatgatccg atggtggttg
    13861 gataaaggga tagatggatt tcgtttagat gcgatttctc atgtaaaaaa agatgaatat
    13921 tcagtaaaag ccacagagaa ccctttctca ccctttcaaa atgtctcagg aatcgaagaa
    13981 catttgaccg atttgaaaca tgtttttgaa gagtacgata tcatgactgt cggagaagca
    14041 agtggtgtca ctgcagaaga aggaccacaa tgggtaggga aagacggtta tttcgatatg
    14101 atattcgaat ttgatcatat ccatatctgg cagcaagaaa aagaagggca attagatgtc
    14161 cttaaattga aacttgcatt aagcgcttgg caaacttcat tggatggtat cggatggaac
    14221 gctttatata tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg agatactcgt
    14281 cccgattttt gggctatgtc tgcaaaagca atcgctatga tgtatttctt tttgcaaggt
    14341 acgccattta tttatcaagg acaggaaatc ggaatgacga atatgccctt tgaatcgatc
    14401 gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag
    14461 acaagagaag aagcattgga tatcattcgt gaaacaacga gagacaacag caggacaccc
    14521 atgcaatgga cttcggaaca atatgctggt ttttctacac atgaaccttg gctgatcact
    14581 aatccaaata ccaaaacgat caatgtagaa caacaagaat atgaaccaga gtcggtcctg
    14641 caattctata agaacatgat ccgcattaga caaaccaata aaggtctgat atacggttct
    14701 tataaagaat atcttcatga acatccacaa ctttatgtct atgaaagata tttagaagat
    14761 gaacaatatc ttattatggt aaacttaaca gaatcacttg ctgactatga acttccaaaa
    14821 gaagcagatc agtcctggac attactcttg tcaaattcct ctagcggaga atttgaagct
    14881 aaaggcatac tagcacctta tgaagctcgg ttatataaaa caaacaagta aaacttcctc
    14941 gttaaagttc attagttgga ctttgcgagg aagtttttca gattgctctg tcaattactc
    15001 atttcaataa ttcgctctag gaccaccgat atatttagga cgagagcgaa gggctgtcac
    15061 gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt acttggacat gtttgatatg
    15121 cataccgatt gatgtgtcac cgatatcgat accggcttgt ccaactacgt gttccacttc
    15181 gacaggatca ttgaactggt caaaagcagc aacagaacaa gcacctccag catgtagtgc
    15241 aggaacaaca gagacaattt cgaaattttt tgcttctgcc actgttcgtt ctactactaa
    15301 tgctcggttc aaatgttcac aaccttggac agctagcgca atttctttag gatccaacaa
    15361 ctcttttaac gtacggatga tccattgacc aacttccgca ctggaatttt ttccaatatg
    15421 tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg
    15481 tgcctcagcc aatatgtcat taactatttc agtcaattgg tcttttaatt ctttttctga
    15541 caacttcatt ttctaaaacc ttctttttca attaatattg cttccgtgct tgaatacgtt
    15601 gaagacttgc agttatagga aaggcaagtc ccattccaat cagattttgg aagatgtttc
    15661 ctggtataga agccactcca gccggccaat catataaaat ccaaccggcg ataccatagc
    15721 cgatgatcat aactaagctt cctaataaaa gccctaatcc ccacatacga gtagaagatt
    15781 tgctgaaaga accagcaaca tatccttgta acccgtggat cactaatgaa aaaactgccc
    15841 actgcggata tcctgaaatc aaatcgatca atagaccact ggaagcacca acaactaatc
    15901 cacccgttgg accataaaga aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa
    15961 atccattcgt agccggtaca gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa
    16021 tcagcatagc atagatcgtt aattgcttag ttttcatttc tcattcctcc tttttgaata
    16081 ccgccagacc agacacttcc ggtatgatcg ttcatcagca cgccattaga aatacaatgg
    16141 gcaacaaatt ttttactttc tgctactgct ttggtaaaat ctgcccctaa cacaagattc
    16201 gctgttattg cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt gtcgattttt
    16261 ttatgagaaa atacctcata atgatcaatt gaatagagaa gatccacagc ttcatctcca
    16321 gccatccttg tgccgccttt gatcacaatg ttttttgttc ctgaagcagt gagttcttta
    16381 gcgagatgga tcatttctgt taacgaagag ggaagagctt gaccagaaag taaagcagct
    16441 tctgccaaat taggcgtaat catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa
    16501 atatacaatt gattgatcac agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg
    16561 attgggatat tttgttttgt ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa
    16621 ttcaataacc caattttgat accatctaat gaagtcattt tatcaatcga aactaactgt
    16681 cgtttaagca attccaaaga tatagattcc agcataaatc gctcattaat atctagtgcc
    16741 cctatagaag tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt ttttaaatct
    16801 gtttggattc ctccaccagc aaatggatca gaaccgccaa ttgtcaagat tcttttcata
    16861 acattttcct ccatgaataa gagtataacg aaattttcaa aaaagaaaaa cagccaattg
    16921 gcatattttt atctcatcca atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc
    16981 tttctttcct taaatagtta ggacttttgt gattttaatt ctttctcgat ttgaagccgt
    17041 tgcactttca ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat agggacgttc
    17101 aagcgaggaa gttcatggac ttgctcccac catcgattcc aatccatttc tttatcagga
    17161 gctaaagcaa taatcggttg tggagaatga tttttatccc ttacaatcac tacttctgcc
    17221 aaaaaatcta acgaatctaa taaaaagtct tcaatagcta atgtactatt gatcgtttcg
    17281 atcaaatcca cttgccgatc tttcaaaaat aggtggccat gcttatccat aaaaccgtaa
    17341 tctccgctat cccaccaatc gccataaaca ttttcctgga accgtgcatc ttccttgtaa
    17401 taagtcaaag cccgtccttt agacagtaat tgtatatgac catctgtgtt ttcaggtagg
    17461 acattcccaa ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc aacgcccata
    17521 tctcttgcat cacttgtttt caaactctct agtgtatgag ccttaagaat cattggtccg
    17581 cattcacttt ggccatatac ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa
    17641 aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg agtgatagta tcggataccc
    17701 gaaaaagctt ctggatgctc cttagcagta aaacgccact gaacaaagtt attcggatgg
    17761 gtctctagtg caattggccg gtgagcttcc aacatatgaa caacttttga actttgtgcg
    17821 tttgctaaag gcatcatagg aaatcccatg gacatcaaag aagagatacc aatattgaat
    17881 ctggaatgca ctggagagat gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa
    17941 actgtttttt gccatttcgt ccgccatccc atagaatgag cagaatgaca aataagttta
    18001 gggataccag ttgtaccaga agtatgggtc atataagaaa tttcattttt gtctaaaaat
    18061 gattgagaaa cagacaaagc aggttgttcc attaaataaa gaatagg
//
LOCUS       DACDHJ010000036        17935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDHJ010000036 DACDHJ010000000
VERSION     DACDHJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.037"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            143..1135
                     /locus_tag="IV223_000981"
     CDS             143..1135
                     /locus_tag="IV223_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8711285.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1195..2616
                     /locus_tag="IV223_000982"
     CDS             1195..2616
                     /locus_tag="IV223_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8711286.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2609..4108
                     /locus_tag="IV223_000983"
     CDS             2609..4108
                     /locus_tag="IV223_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8711287.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4147..5541
                     /gene="uxaC"
                     /locus_tag="IV223_000984"
     CDS             4147..5541
                     /gene="uxaC"
                     /locus_tag="IV223_000984"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8711288.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5700..6383
                     /locus_tag="IV223_000985"
     CDS             5700..6383
                     /locus_tag="IV223_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8711289.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6385..7221
                     /locus_tag="IV223_000986"
     CDS             6385..7221
                     /locus_tag="IV223_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8711290.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7232..8986
                     /locus_tag="IV223_000987"
     CDS             7232..8986
                     /locus_tag="IV223_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711291.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9262..9603)
                     /locus_tag="IV223_000988"
     CDS             complement(9262..9603)
                     /locus_tag="IV223_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8711292.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9671..11842)
                     /locus_tag="IV223_000989"
     CDS             complement(9671..11842)
                     /locus_tag="IV223_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8711293.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            12053..12910
                     /locus_tag="IV223_000990"
     CDS             12053..12910
                     /locus_tag="IV223_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8711294.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(12981..13838)
                     /locus_tag="IV223_000991"
     CDS             complement(12981..13838)
                     /locus_tag="IV223_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8711295.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(13823..16525)
                     /locus_tag="IV223_000992"
     CDS             complement(13823..16525)
                     /locus_tag="IV223_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8711296.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(16538..17827)
                     /locus_tag="IV223_000993"
     CDS             complement(16538..17827)
                     /locus_tag="IV223_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8711297.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         5250 a         3660 c         3386 g         5639 t
ORIGIN      
        1 agaatagcat aattaaaaat aaaaatccaa gtaattttta acgtgtgcaa tcttttcata
       61 tcaaagatct attgacttat cgtagttatc gtaatatgat gaatgcgtta acaaaaagtg
      121 aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat gtcgctaaag cagctggcgt
      181 ttctcatacg acagtttctc gtgctttgaa taacagcagt ttaataaaaa atgaaacaaa
      241 acaaaaaata cgacaactag ctaaggaaat gaattattcg ccaaactata gtgcaaaagg
      301 tttagtgaat caaaaaaaat atctaatcgg actatttttc tcaagtttgc ataaaggaac
      361 ttcatcaaat ttcctagcag attccattgc aggcatccat tcagtattag acacaaatta
      421 cagtttatct gttgaaagta tcgatgttgt tgatttaaca gaaatcaacc tgcaacgcta
      481 cgatggactg atcgttatga gtcaaagtga tgatgatcag cctttcatcg actatctcaa
      541 agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc aatgatccag cggttatcaa
      601 cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta gattacggta ttggatgcgg
      661 acatacgaag atcgcttata ttggcggaaa gaaaaatttt cgttcgacaa aagaacggga
      721 aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct gtcaatccag cctatttttt
      781 aacaggagat tacagcttag aaagcggcct ccaaaacatg gaatacctat tatcactttc
      841 tgacctgcct actctggttt tttgcggcaa cgacgacata gcgattggtg cattgagagc
      901 tgtacattta gctggcctcc gtgttcccca gaacatttct ctgatcgggt tcgatgattc
      961 tccagtcgtc tcctatctca atccgccgct taccaccgta cgtaagcctt tgaaagaaat
     1021 gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa ggcaggacga tcgaacaatc
     1081 taaaattgag ctctccacct tgttgatcct tcgtgagtcg atcaattact tgtaaactac
     1141 aagtaatttt atataaaaat gttaacgtgt gcataacaca gaaaggaagt ttatatgaaa
     1201 aaactgacaa aagaattagt gaaaaaaaac gaagcacctg taaaagttct tcagtttggt
     1261 gaaggcaatt ttatgcgtgg atttattgat tggcagattc agcagctgaa taaccaacaa
     1321 ttgtttaaag gtaatgttgc aatcattcaa cctttagaac aaggtcttgg aaatatgatg
     1381 aaccagcaag ataatttata tacggtcatt ttacaaggac tcagcaatgg cgaaatcgtt
     1441 gatacttccg agattatcac cgtagtagaa caaatgatca atccttatga tgaatgggat
     1501 gcttatctat ctctagcaga aaatgacgac ttggcctata tcgtctccaa tacaacagag
     1561 gcaggaatcc aattcgtgga gactgataat ttaacagata ctcccccaca gagcctcccg
     1621 ggcaaactga cagccttttt ataccgacgt ttcaaattaa acaaagctgg ctttaccatc
     1681 attccttgcg aactgattga cagaaacggc gaacgtctaa aagaaatcat tttacgctac
     1741 gcagaaaaat gggatttaga acctgctttt gttgagtggt tagaaaaaga aaatgttttc
     1801 tgttgtagtt tagttgatcg catcgtccca ggttatcctc gcgaagatgc aggtgttttc
     1861 aatcaacggc ataactatga agatcaattg atggtcaaag cagaaccatt catgctttgg
     1921 gtgatcgaag ggcctaaaga attggagaat caactaccat tcaaacaggc tggcttgaat
     1981 gtcatcatca cagatgatat gactccttat cgtgaacgta aggtgcattt gcttaatgga
     2041 ccgcatacag cgatggtccc tcttgctttg ttggcggaat tggaaactgt agaagaagta
     2101 atgaaagatc ctttattctc cgcatatgtt gatcagttat tcaatctaga attgattcct
     2161 atgctttctt tacctaaaga tgaattagcc atttatgctg accaaatcaa agaacgtttc
     2221 ttgaatcctt ttgcccatca caaattagaa gcgatttcat taaatagtgt ttcaaagttc
     2281 agcacccgtc tgcttccagt gttcaagaag tatatcgaag aacaaaatca ggtaccaccg
     2341 ttaatcactg tttcactagc tgcgctactt cttatgtatc gaggcgatca agtaaagcca
     2401 catgatgatg aaaagacgat aagcgaattt actgatgctt ggtctgataa tggaacagct
     2461 attcctcgat tattgcaaaa cgctgcgctt tggggagaag atttgtctca aataccgaat
     2521 gtgacagata cagttcaaga aatagctgca aagatcgatc aaaaaggtgt ccggtcagta
     2581 ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc ctgcaaccaa caatgaaatt
     2641 aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt ccaaaacaaa cacaactgac
     2701 gattgatcag caaacgatca ccactcttga agatatcccg caaggtcata agattgcatt
     2761 gggtgatttg aaagctggag acaacgtgat caaatatggc tatccaatcg gtcatgtgac
     2821 gacagacgta actgccggtc aatggctgca cactcataat gtaaaaacca atctttctgg
     2881 tgaattggat tatcgatatg aaaaagatgt ccatccaagt cattaccttt ttgagaaccg
     2941 aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg attcgtaatg atttgttttt
     3001 agtacctacc gttgggtgtg tgaatggcat tgctgaactg atcgtcaaac agttcaagga
     3061 aaaacatcca gatctgggac agtttgacca tattacgatt ttaaagcatc cttatggctg
     3121 ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt ttagcggacg cagtaaatca
     3181 tcccaatgcc ggaggagttt tagtatttgg attaggttgt gagaacaata cagttcctga
     3241 attcaaaaaa attttaggtg aatatgatga agaacgagtg aaatttctag tggctcaaga
     3301 tgtttacgat gagatcgaac aaggtgtggc tctattagaa gagcttttaa cagcggcgga
     3361 aaatgaccag cgcatatcag tcccgctctc taagttgaat gtcggtttga aatgcggcgg
     3421 ttctgatggt ctatcgggaa taactgctaa tcctttactt ggtgcatttt ctgactactt
     3481 gattgctcaa ggaggcagca cgatcttaac agaagtgcca gaaatgtttg gcgcagaaca
     3541 agtattgatg gcacgagcgg aaaatgaaga agtttttgac tcaatcgttg acttgatcaa
     3601 tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt tacgaaaacc catctcctgg
     3661 taataaagca ggtgggatca ctacattaga agataagtct ttaggctgca cgcaaaaatc
     3721 aggaagctct cctgtcgttg atgtgctaca atatggcgaa aaaatcagaa aaccagggct
     3781 tagtttattg caagctcccg ggaatgatct tgtagctgcc tctgctcttg cttcatccga
     3841 ttgccagttg gtcttgttta ccacaggaag agggactccg tttggctcat atgtcccaac
     3901 ggtcaaggta tctacgaata caactctttt tgaacgcaaa ggtcattgga tggattttaa
     3961 tgcaggcgtc ttattagaac aaccaatgga agtaatctta gaatcgtttg ttcaaaaaat
     4021 catcgctgta gctagtggag aagaaacgaa aaatgaacaa aatgacgttc gagaaattgc
     4081 catttttaaa aacggagtaa cactttgatt ttcataaaac agtataaaaa gaaagaagga
     4141 agatttatgt ttttatctga tgatttttta ttaaaagacg attgggcgaa aaagctttac
     4201 cattcctacg caaaaaacat gcccattatt gattatcact gtcacttaga tccaaaagaa
     4261 atttacgaaa acaacaattt cagcaatctg acagaagctt ggttaggagg cgatcattac
     4321 aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa aaatcacagg aaatgcttct
     4381 gattttgaaa aatttttagc ttggtgtcaa accgtaccaa aaattatcgg aaatccgctt
     4441 tacagttggt cacatcttga attgaagcgt ttcttcggta ttgacttgtt gataaatgaa
     4501 gaaaatgcag agaaaatttg gacacaagca aatgaaattt tggcagctcc tgattttcgc
     4561 cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt gtacaacaga tgatcccgca
     4621 gacgacttgc actatcatca gttgcttgct catgaagaac cagatttgaa ggttctacct
     4681 tcattccgtc cagataaagc actcaatatt gaaaaagaag gatttaatgc atgggtcaca
     4741 ctactggaag aagcagctga ccaaacgatc accacctatc aagaactgat agaagtctta
     4801 gggcagcgga tagaatactt ccatcaacat ggatgtcgtg tttccgacca cgctttagac
     4861 atcttacgct ataaagaagc ggatgaaaca gtattagaag agatttttca aaaagcacga
     4921 gctaacaaac tattatcttc agatgaaatc gatgcttatc gtacagaaac actgatacat
     4981 ttgacccact tctatcattc tcataattgg acgatgcagc tgcacatcca tgcttatcgt
     5041 aactgtaata cacaaatgtt ggaaaaatta gggcccgata ccggctatga tggaatgaat
     5101 gatctttctt tgactattcc tctgcaaaaa ttattgaacc gtgcagaaga aacggatcag
     5161 ttgccaaaga cgatccttta ttcattgaat ccgaatgact atcctgcgat tttagctttg
     5221 atgggctgtt tccaaaaaga acagaatgga aaactgcagt taggttctgg atggtggtac
     5281 aacgatactc gagctggtat gcgcgatcaa ttgacacaat ttgctgatgg tagtgctttg
     5341 gggaattttg tcggcatgct gacagattca agaagtttcc tgtcctacac tagacatgaa
     5401 tatttccgtc gtgttctttg tgaattttta ggcgaaatgg tcatgcgagg agaagcacct
     5461 gaagatgaac aacttttagg aagtctagtt caagcgatta gttatacaaa tgctaaaaat
     5521 tactttggct tttttgccta attgattaat agtaacagta acgaatcaaa aacaggatag
     5581 tgaaataacg gaatccgttt atccacgttt tagagaagaa gacggaccaa atttgctccg
     5641 tcttcttctc ttgctgttct gcttcattgc ctataaacct ttatgaaaga agagataaca
     5701 tgcctactat ccatattgcc ggtgattcta ctgcggcaga aaaagatgaa aacaagcgtc
     5761 ccgagacagg ttggggagaa aaaatctctg cttatttttc acccgagatc cagatcaata
     5821 accaagcaaa gaatggacgc agcaccaaat catttatcca ggaaggaagg ttagcccgtc
     5881 tagcttcaca gtttcagccg aatgatttct tatttgtaca atttggacat aacgaccaga
     5941 aaatccaaga agaaagaggt acgcttccct tcggtagcta tttaaaaaat ctgcagatat
     6001 tttttgaatg cgcaaaaaaa gcgaacgtcc aaatggtctt actgacatct gtgacgcgac
     6061 gagattactt agaaaacgga acattgaatc cagatatact tggagactat ccaaaagcga
     6121 tgcgtgcatt tgctgagaaa caccatattc ctcttttaga tgtgttctca agatcacaag
     6181 aattgttcca gaccttctca aaagaagaga caaaaaaatt ctacctgcat cttatacctg
     6241 atacttgtaa aaattaccca gaaggactaa aagacaatac ccactttagt ccagaaggcg
     6301 ctgataaagt tgcccgaata attgttgaat tgataaaaga atcccgtctg cctcttgcta
     6361 attatttaca aaaaggagtt tgagatgttt ccattaaatt taggcatccg tgcccacgat
     6421 ttagatgttc gaaatcgtga agaaatcgtt caaaaagtat cacagcttca cttgtcccat
     6481 atccaatttg caccacaaaa agcatttcct gaacaactta aattaactga catgacactt
     6541 gggagtgctg cctattttgg tgaatacttc caaaaaaaca atatcgaact ttccatttta
     6601 ggatgctata tcaacctatc cagcaaaaat caagaagttc gtgcacaagc attggcaact
     6661 ttcaaacagc aaattgctct gtgctctgcc tatcaagcca agatgattgc tacggaaacc
     6721 ggtagtgtct ctgtcggtta cacaaaagaa aattttactg aagaagctta tcagatcgcc
     6781 agaaattcta tcattgaatt agtcgctcat gcagaaaatt ttggcgtaac tgttgctgtt
     6841 gaagcaggga tcaatcatcc aatctataac tatcagttag ctaaacgttt aatcgatgaa
     6901 gtagcttcac caaatttaaa aatcatttta gattgtgcga atttgatgca taaagaaaat
     6961 catgctgatc aggaaaaagt gatccgaggt gccttggaca atttacatgg ttatattaca
     7021 gcgcttcatc tcaaagacta tatcatggaa aatggaaaga tccgcatggt acctgttggc
     7081 aagggctgtt tagatttccg cccggtactg cattatatca aatatgagcg tcctctaatg
     7141 tacgccactc ttgaagctac tcctgagatt tatgttcctg aagcaataga acacttgcag
     7201 tttatttacc aagattgtta aggagaagat tatggaggaa cgaatacaga aaagttatca
     7261 aaaatatcat gcaaatgctt ttcagacatt gctgcatctt tatcagggaa attattggcg
     7321 ttttgccatg tcaggactct tttttattat caaacatctt ccatcttggt taatgccgat
     7381 cgctattgcc aatgtaatca atgcagcttc atcgaaagat tcttctcagt tgcaggccat
     7441 ttatttcaac gcttttttga tggcttgtct gattggtcaa aatatattga cgaattattt
     7501 tcatgtaaaa taccatagca cttcgatccg tcaagtagaa gctggactaa gagcgaccct
     7561 tatcaagaaa atccaagaac tatctattcc ttatcaaaaa gaattgcaat ctggacgaat
     7621 ccagtcaaag atcatccgtg atgtagaagc tatccaaacg ttgtcctctc aagtatttgt
     7681 cagcggatta aatatcgctg tcaatttatt cgtagcttta ggtatcacgc tttataaaag
     7741 tcctgttgtt ttcatgtttt tcttattgac tgttcctctc gccgcattcg ttactctctt
     7801 tttccgtaaa aggatcagac agtccaactc agacttcagg caagaaatgg aagagacttc
     7861 tgcaaaagtg atcgaagtga tcgaattagc accgattgct cgagctcatg ggttagaaaa
     7921 acacgaaaca actcgcttaa ataagcattt tactcaaatc tatgaaaaag gattccactt
     7981 agatatcctg caatctgttt tcggatcagt cagctgggct agttttcaga tatttcagct
     8041 gatttgtttg attttcactg ggatattagc tttacaaggg cgcatccaac ccggcgatgt
     8101 cgtgatgtat cagacgtatt tcacgacgat cgtcaattcc gtttctggaa tgatcacgct
     8161 gattcctacg atttcaaaag gcatggaatc tatcacttct attggcgaac tgttgattgc
     8221 tggcgatgta gaaacttatc acggtaagaa aaaaatccaa catttggatg gaaattttcg
     8281 ttttgaggat gtttcttttc tttatccgga ctcactcaaa ccgattatcg atcatttttc
     8341 tctagaggtg aaagctggag aaacaattgc ttttgttggg ccatctggct ctggaaaatc
     8401 tacactcctt aacttattga ttggatttat ccagccgact tcaggaaaaa tttttctaga
     8461 tgatcagcct ttggatgaac tggatatgcg aagtgtgcga aattatttag ccgttgttcc
     8521 tcaaaccact ttgttgttct ccgcttcgat caaagagaac atcacttatg gcttgaaaaa
     8581 cgtatcaaaa gaacgtttag atgaagtcat cgaagctgcg caactttctt cactgattga
     8641 ccagcttcct gatggtctgg ataccttagt aggcgaacat ggcaacaagc tgtctggtgg
     8701 ccaaaagcaa cgtatctcca ttgcacgagc gttgatacgt cagccaaaaa tcattttgct
     8761 agatgaagca acctcagctt tagataacca gtcagagaaa aaaatccagc aagccctgaa
     8821 ttacctgaca gaaacaccca ctacttttat tgtggctcat cggctttcaa cgatcaaaga
     8881 ggcagataaa atcgttgtga tcgatgaagg aaaaatcgtt gaaataggta cttacgaaaa
     8941 actgttagct caaaaaggct atttctaccg cttgaaaaat gaatgacaaa aaataggagc
     9001 tatgacaaga cttttgtcgt agctcctatt tctgaataaa cggtgttctc ctgccaactc
     9061 ctcggtcaca agtcacaata gtcatcaaac atgagaagct gttttttgaa attgttcctt
     9121 gtaactcaga gttaaacggt aggctcacga cctcttggta aatggtgttc aagagacagc
     9181 ctatcggcaa taagaagcaa ttttgtcatc gctcaaggcc gaacgtctct ttcacaccct
     9241 ctgttgtttc ataaaatagt tttattcgct gtaaaggtcg cgaactggtg tcatgatgat
     9301 acggttcaag tcattgatga ttgtactgaa gccttgctct ttttgcatca agtcattgat
     9361 tacttcttct gattggattt tcacagccat ttcttgtgct ttcattgcat cttcgtcgct
     9421 gaactcttgt ccactcattt gtttttcttg taattcgatt tgcatcgctt ggaagtcttt
     9481 gaacagtttg tgtgcttctt cgtttgcttg tactttctcg tacgcttctt tcaacgcttt
     9541 gaattctggt aattcacgga tctcacgttc gatttgattc gcgctatcgt agatgtttga
     9601 catgttatgc cctcttttct tttagtagtt actctctatt gtaacatggg gttattcgtt
     9661 ttcaaagggg ctactctcct tgatatttcc gccagaggtc tttagccgct tcaccaattc
     9721 cctgaacgcc ttctttcacc atatcgccga agtttttcag cccttctttt gcttgttcac
     9781 catattgatt caagttatct tgccaatttc cagactctcc attagaattt cctgtgtttc
     9841 caacttgatc tgcagctacc acttgtcctc ctgtggcata ggcatctgct acactaaatc
     9901 ctgtttttga agtatacgga aggatgcctt ctgccgctga tttgaagact ttcccgacaa
     9961 catttccact tgtcccttcc aaatagtgca gcttgctgga ttcttcgtaa cccatccatg
    10021 tcgaaatcac tacgtctggc gtgtaaccga tcatccattg gtcatttgct tttgtggcat
    10081 cgaaattagt ttctgtggtt cctgttttcc ctgctatcgt ataacctgca ggattcgcag
    10141 cagccgctgt tccatttgaa aatgtaccaa gaagcatgct ggtcatttca tcggctgttt
    10201 cctttgatat gacctgtttt ggttttttgt ttgtgttatc gacgatcacc gctccagtcg
    10261 aatcaacgat tttcgtgata agatgcggcg tatacatctg accatcattg gcaaatgcac
    10321 tgtaagcacc cgccatgatc aatggagata ctcctttttc cagtccacca agggctaagc
    10381 cataatactt atcagatttt gctaaaggca ggccgaattc ttctgcttta tcatatcctt
    10441 tttgtaaacc gatctcatgc aaaagccata cagtaggcaa attcaaactt tgtgcaacag
    10501 cttgatacat cggtacttct ccttggtatg tcccgtcaaa attatgcgca tcgtaataac
    10561 tttgcggttc gtctttcaag atactggaag gcttgtatcc agcttccagt gctggtgtat
    10621 atacgcttaa tggtttgatc gttgatcctg gtgaacgacg catctgtgta gcaaagctga
    10681 atcctctaaa gacatgatct cctcttcgtc ctactagtgc ttgtacacca cctgtattcg
    10741 gatctaaagc tactgaagca gattggacca tggcaccatc tgaagcattt gctgggaata
    10801 atgcactgtt ttgataagta gcttctaatt gcttttgata atcctgatct aaagctgtat
    10861 agattttata gcctttattc atgacatctt cttcatcaag accatactcg ttaactgctt
    10921 catcgatcac tgcatcaaaa taataaggat aacgataacc agaatctgaa ttatcgtatg
    10981 tgtcattaag atacgcgctg atatctgtct ggctttctgc ttgttcttgt tcttttgtga
    11041 tttttccgtt ttcttccatt acacttagta ctgtgttttt ccgatcattc gcattttttg
    11101 ggtgatcgat cggattataa ataccaggac ctttcagcat tcctgccaaa gtagcagcct
    11161 cagacagcgt aacattcgtt gcatctactc caaaatactt tctggaagcg tcttggacac
    11221 cccatacccc gttgccaaaa taggcattgt taagatacat ggtcaagatt tcttctttgc
    11281 tgtacttctt ttcgatctcg attgccaaaa ataattcctt cgcttttcga tcaaacgttt
    11341 ggtctaatgt cagatacgca tttttggcaa gctgctgagt gatcgtactt cctccgcccc
    11401 ctgatgttcc gccagacaag accattcgga ctgctgcacg ggcaatccct ttgatatcaa
    11461 acccgtgatg ctcgtaaaaa ttcctatctt ctgtcgagat tactgcatct tggatatagg
    11521 gagaaatcgc gtttaattct acatatgttc ctttttgggc gtacagtgtg ccagcttctt
    11581 cgtttttctt atcataaatg actgtcgatt ggcttaatcc tgattttaac gtttcgacat
    11641 ttgcttgttt tgctaaaata aagaggtaga tacttgtcac taagacacag atcaaaccaa
    11701 tcagcaaaat caatttgttc acatgatatt ttttccagat gcgctttcgc catcgatgga
    11761 attgtccgag atatggtctg atccaacgcc aaaaactgat caaagcagct tttatctttt
    11821 ctaaaatgct ttgaaaatcc atttgcttct tctcctgtct atttgattca atcgtcttat
    11881 tttaccatag tgaaagagtg ttttcttcct gcttaagttg tagttcaata tgttttttta
    11941 cgttagtgca agtagagtaa cataagaatg aacagaaaaa tgtaagtttt ttcttaaata
    12001 tgttttaaaa atggtacaat tagtctgcga ttgcactgaa aggagtacac ttgtgaaaat
    12061 cacgatcact ttacctatga accatcctga aacaacaatt cgcgacttgc ttgaaaagga
    12121 atggttagtt cctagaaagg ttcgacactt tttgcgtata cgtaaaaacg tttggatcaa
    12181 tcaacaacct gctttgttcc atcatgaagt aaagggcaat gaccagatca cattgttatt
    12241 cgaagaaaca gattataatt atcaagaagt tcaattagga gatgcgaagc acatccgtgt
    12301 tttatatgaa gatgagcatt tgattatagt ggataagcct gcaggaatga agactcatcc
    12361 aaatgaaccg acagaaaaca gtaccttact caatcattta gctgcttatc tcgacactaa
    12421 acagcaacga ccttacgttg tccaccgctt ggacaaagaa acaagcggtg ccgttttatt
    12481 tgcaaaaaat ccatttgtct tgccgatact tggaagaatg ttggaacaaa aaatgatcta
    12541 tcggagatat caagccatcg tatggggaga aattgccaaa gatatgacca tcaccaataa
    12601 aattggacga gatcgccatg atcggagaaa aagggtcgta gatgataaaa atggtcagag
    12661 cgccttgaca catgtcactg tcaacaaagt actttctgaa caaacacaag tctattgtgt
    12721 tttggaaacc ggacgtacac accagatcag agttcattta gctcataagg gacatccgat
    12781 cgttggagac cctctctacc aaaatagaac agctgaacga ctgatgctcc atgcattaga
    12841 actgcacatg atccatccat ttactcaaga aaaaatagtc ggtaaagctt tacctggatt
    12901 atgggaatag aaataaaaaa gaacagagtc ttatcagcag ataatgataa gactctgttc
    12961 tttttgtgta atttgtctgc ttacttttga tgttcgtgtt ctgctaacag caaactaccg
    13021 ataatcccac tttcattcgg cagtccccaa cgaacgatat attcacttac ttgaggtgtt
    13081 tccatataac cacccatcaa ttcaacaaat tgctgacgga ttttttgaag gagatgatcc
    13141 tgattcatta ccccaccgcc taaaataatc ttttcaggtg ctaacgtcaa cgtatagttg
    13201 atcagtgctt gagcaatata ataagcctga atagcccaaa caggatgatc ttgcggcaga
    13261 tcttgccctt tgatccctgt gcgtgcctca atagacggtc ctgctgctaa tccttctaaa
    13321 caatctttat gataaggaca agtaccttca taatggtcat ctggatggcg tttcacccaa
    13381 atatgtccca tttcaggatg tgcgatccct gaaaaaattt cttcattgat cacgacacct
    13441 gcaccgattc ctgttcccac ggtaaggtaa atacaactat tttttccttg tgctgctcct
    13501 tgcttcaatt ccccatatgc agcagcattg acatccgttg tccaagcagc aggaatattg
    13561 tagcgttcct tgattgtgcc aagaaaatcg aattgtgccc accctttttt gggagttgcc
    13621 aaaacgtatc cgtaattatc ttttgttttg tcgataccaa tcgggccgaa tgagccaatc
    13681 ccgattccct tcaattcata acgatcaaaa aattcaaata cctgatccaa tgtttcttct
    13741 ggtgttgtgg tagggatact taatttttct aaaatttctc caccatctga aaccgcacac
    13801 acaaattttg ttccgcctgc ttctattccg ccatacattc gttttcctcc tcgaatccga
    13861 ttgtccgaat ctcataaggt tggattattg ggacaaccgc ttgcttcgtt ggttcttcaa
    13921 gcagattcaa tatgacagtt tttccattgt ctttagttat ttccaatttt tctaaatgac
    13981 tagacatatt gaaacctctt actactactt tatcactaaa cttgctgcgt ttcaaagctg
    14041 taattgcaaa cgtttcactt tttatcgtta aatattgttg ttttgaaatc aatgttcctt
    14101 catgatgttt gatttgttga gtggaaaaag gaatctgtgc tgcgtatgca tgcaaataag
    14161 tagagaattt ttcttctggt ccatgtagtt caatgctgta attgaaccga tgctcaccta
    14221 gacactgtgc ttctggtgtt ggaaaatatc cccaatcgcc tagttctccc acactgcgaa
    14281 gtagcgtcac tgcgatttgt ccgtcttctg ttacttcata ttcattcaaa ccaaagtttc
    14341 cgactgttac accgtactct tcatcatgaa tattgacaaa tgcttgctga tgttgcggat
    14401 tcgtggggtt ttcccaactt ttagacacat ggtttgggcg cttcaccact tcaaaaatac
    14461 tatctgcttc gtgatgttcc acatgtaact ttgtaggaaa caagacacgt aagcgatgat
    14521 ctttcatctg attgtcgaga gtcgtttcaa agtcgatttt tttactatct tttctgattg
    14581 tcatgattgt ttttatttga aggattcgtt tttctttgga tcgttctgct tttcgataac
    14641 gaaattccac aaccatctgc tgttcttttt ctaaacgctc gtctgcagaa acagggattt
    14701 ccatttcctg tagaatactt atttttgcta tttccggtgt atctactaat acttttactt
    14761 cagagttttc tttgttactg gataggattg gctgatcaca gaaaggttgt ttaaagatat
    14821 actcatttcc tatatcgccg atatcttcaa aaatatgcag attattcaac tttcgtttgt
    14881 ttttgcgatc ttccattgta atggtaccat ttttttcaat cgtcaaatgt agagggccgt
    14941 tttcgatgat tcgtccactc tgatgaatca ttgtttcctt ttcttctgct ttcgcttccc
    15001 cttctacaag cgcgaatgtt tcccaagaaa atgctggcat gttttctaat ggtaaagtca
    15061 ctttgatcat tcttgccata tatggcacac ggaaacggtc tttcggcaaa tcataaccaa
    15121 attggacagt ttcttcagat aaaatagctg gtatagtggc cccacttttt gtttctacat
    15181 gatacttacg tttaggcaaa ttttctaact cttgatatag ttgttcaggt attccttcag
    15241 cgaatctttt tctttctaat tcgatggtta tttctgcttc accagtttta tccattcctg
    15301 cagtatttac aatgacaaat ggaaagctgt cttttgggaa tccggtcgta tcgattgctg
    15361 cggtcagctg ttctaaggaa tcttgtgcaa gatactttcc aacttcatct gctttttcaa
    15421 aacgaggtat catttcccga tggacagaat cgactgaaca accgcaaata ctgtcgtgag
    15481 gatgattttg cattaatgtc ttccaagcat aatctaattg atcatgggga tagtttccac
    15541 taacttcata agccatcgtc gcaagtggtt ctgtaatatt ttctagctgt cgctggacct
    15601 tggtattcca ttgttttaag taaacccgag cagatgctgt attggctaaa gtataccagc
    15661 catccgtttc ttgacttgtc aattctcctt ctactgagcc taagtcttct ggcacatctt
    15721 tttgaactgc ttccagatag tcagtaaaat tagaatgaat aaactcgtaa tctggaaaca
    15781 gttcatttgc gagatggatt gcttttgata tatctttttg taccggttga tgatcaacac
    15841 cattcatcat caataagtgg ttcgtagagg catattgttc tgcatccgct aatttttgct
    15901 tccaaaaggc aagagctgct tctttttcta ctggaatctc attcccgttg ctataccaat
    15961 tggcaaatag cagaccaaag attgctgttt gatcaggtcc tttccaccac atctctgagt
    16021 attgggaaga atagttttct gcttccaaaa cttgattatc aaagccaatt ggttttacac
    16081 cacggccaaa cgctgctgct gaaataccag cttgtttcat taattgaggc gtctgtccca
    16141 tatttccaaa tgtatcggga aaatagccta acatgacagg ggttccccat ttacggcttt
    16201 cttccatgcc aatcagcata tttctcacgt tcgattccga gctgataaga aaatcatctt
    16261 gcaagatata aaatggtcca attctcaatt ttccttcatt gattgcttgt tggattgcct
    16321 gtcttttttc tggccttact tgaagatagt catctaaaat aatcgtttgc ccatccaaat
    16381 gaaaactgtt gaaggacgga tcaacttcaa atagctctag tagatcgtct atcaattcaa
    16441 tcagtcgcat gtgatgctgt tcgtaagcca tgtaccattc acgatcccag tggctatgag
    16501 aaatgatata tactttcttt ttcatttacg atacctccta cttttctact ctaatatcaa
    16561 agtaatccat gactaattcg caaaacatca tatttgccca agaaaaccat tctctcgtat
    16621 aattttcagg attgttcaca tcgaatcctt cgtgcataag gtgtgttcct gcgtcagttg
    16681 ctacaagtaa attcaatatc cgctcttttt cagatttgtc tgacgttgtc attccttcca
    16741 tagctaatgc aatcggccaa acgtattttt ctggcgtatg ggaggatcca attccttttg
    16801 cgtactttcc ttcatagaaa tatggatttt ctttactcaa caatgttctt cttgtttgca
    16861 gataatcttc ctcttgttca gaacaatatc ccaagtaagg tgcggcgatc agattgggca
    16921 cattactgtc atccatgact gtggcgtttc ccattccatc tacttcatat gcataaatcg
    16981 tttgcccttt ttgattaacc gttcgtccgt gattctgaat cccctcttgg atctctttct
    17041 tcaatttctg tgtttgctgt acaatttctt tatcatcgag tatcgtttga aaaatttctt
    17101 ccacataacc taacacgacc accgcaaaca tattggatgg taccagatat ccataacggc
    17161 aagtatcatc gctaggacgg aaaccagacc atgtcattcc tgtaaaagct gtttttgcac
    17221 ctcgcccgtc attgatcagc gtatcttctt ttcttgtcgt gtctcgtaca aatgtataag
    17281 gagagtgttc atgatcttgc tctgttttga acacgtccaa gatttttttg ataccttcaa
    17341 taaaagattg attgaattga tcagtttttc ctgtattttt gtaaagaaga taggccaact
    17401 gtattgggta gcataaagaa tcaatttcat attttctttc ccagatccaa tcgttcattt
    17461 ttgtatgatc tgtttggtgg ccagcaccgt tagcttcttc gttgaaggca ttcgcatatg
    17521 gatcgatatt gatataaaaa aattgctttt tgactaaccc gctgatcatg tcagctaatt
    17581 cctcatcttc ttttgcaatc actaaataag gacgaacttg tgcagtagag tctcttaacc
    17641 acatcgctgg aatgtctccc gtcaataaaa aggtggttcc gtcattttgc tttttgactg
    17701 ttgtcaacag agtattggca aatgctgctg taaaattctt tgcccagtcc gcatggatat
    17761 ccccacattt ttgtgtgata tcttccataa atttctgcac cgattttggt atatctttat
    17821 aagccactaa taaaaccttc tttctatttg atatatcttt aaagattata cttcatacag
    17881 ccaattatgt ctacgctttc aaaaaatatc tttttattta ctttttcatc catta
//
LOCUS       DACDHJ010000037        17924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDHJ010000037 DACDHJ010000000
VERSION     DACDHJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.038"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            58..246
                     /locus_tag="IV223_000994"
     CDS             58..246
                     /locus_tag="IV223_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711298.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
     gene            complement(890..1843)
                     /locus_tag="IV223_000995"
     CDS             complement(890..1843)
                     /locus_tag="IV223_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8711299.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1866..2621)
                     /locus_tag="IV223_000996"
     CDS             complement(1866..2621)
                     /locus_tag="IV223_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8711300.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2618..3577)
                     /locus_tag="IV223_000997"
     CDS             complement(2618..3577)
                     /locus_tag="IV223_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8711301.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3570..4520)
                     /locus_tag="IV223_000998"
     CDS             complement(3570..4520)
                     /locus_tag="IV223_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8711302.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4670..5122
                     /locus_tag="IV223_000999"
     CDS             4670..5122
                     /locus_tag="IV223_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8711303.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5211..7478)
                     /gene="gshAB"
                     /locus_tag="IV223_001000"
     CDS             complement(5211..7478)
                     /gene="gshAB"
                     /locus_tag="IV223_001000"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8711304.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7665..8663)
                     /gene="ftsY"
                     /locus_tag="IV223_001001"
     CDS             complement(7665..8663)
                     /gene="ftsY"
                     /locus_tag="IV223_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8711305.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8674..9483)
                     /locus_tag="IV223_001002"
     CDS             complement(8674..9483)
                     /locus_tag="IV223_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8711306.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9492..13073)
                     /gene="smc"
                     /locus_tag="IV223_001003"
     CDS             complement(9492..13073)
                     /gene="smc"
                     /locus_tag="IV223_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8711307.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13093..13779)
                     /locus_tag="IV223_001004"
     CDS             complement(13093..13779)
                     /locus_tag="IV223_001004"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8711308.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14127..15908)
                     /locus_tag="IV223_001005"
     CDS             complement(14127..15908)
                     /locus_tag="IV223_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8711309.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15934..16848)
                     /locus_tag="IV223_001006"
     CDS             complement(15934..16848)
                     /locus_tag="IV223_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711310.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16875..17837)
                     /locus_tag="IV223_001007"
     CDS             complement(16875..17837)
                     /locus_tag="IV223_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711311.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
BASE COUNT         5017 a         3850 c         3163 g         5894 t
ORIGIN      
        1 gagggttctt atattgaagt aaaaacactc tttgtcaatg aagataaaac caaagcattg
       61 gatatttata aaaaattagc ttcaaaaaaa acgaatagtt ttggattgat attaagcgag
      121 tataaaatca aagcagaaga gagttatttc tatcaattac taaaacggtg gagtaagctt
      181 cccgccgatt tttatcgaaa aatgcagatc atcaactatc aaccgcttgc tgagacacat
      241 gcctaaatag aggttgtgaa atcagtgctt tgagctaata ccgaaggtca gctgattgaa
      301 cattgtttag aagagattgt gacaactgcg atttgctttg agtaataaga acgctggaca
      361 gaaaatagct tctcatattt tctagtctag tgggcttatt gccgagaagc agcagatgga
      421 acatcgttta gaagagattg tgacaactgc gatttgtttt gagtaataag aacgctggac
      481 agaaaatagc ttctcatatt ttctagtcta gtggacttat tgccgagaag cagcagatgg
      541 aacatcgttt aaagaagaga tcggtacaaa ataaaaatat ctaaatttaa gttttatcta
      601 agtttcgctt tgtacctttg attcaaatca aaggaggcga tcttgatgct gaaaaaatgg
      661 atcacactat tttttgccac tccccattct agaatcaacc cgtatgatgt agataatgaa
      721 ttcacgttga aaaaagattt ctcaaataaa taaaaaccat ccgtaaaatc atcaatacga
      781 ttttacggat ggtttttgat aacttctttg aggataggct cttgctattt aagaatgtaa
      841 gaaacattat tgttacatag tatgagagtt taatcctctg ttatttatgc tatttaaatg
      901 cttgattcac atcttcgatc atcagtttca ttgattctaa accgcccccg cttaaatacc
      961 agacatctgg ttcaagggag atgatctgct gatttttacc agcattcgtt tgtgctacca
     1021 gttcattggc agaaatatca tctttgctgt cgtctccacc aatagctttt gttctgtcta
     1081 ctacaaataa gatatccgga tttttttcca gcacatattc ataagaaaca ctttgaccgt
     1141 gagtggatgc ttcgatttga tcatctgctt gttcaaagcc aaataaatca tggatgaaac
     1201 cgaatctaga accagatccg taggctgata attgcccttc attcacaagt gtgactagtg
     1261 cttttttgtc agtagcttct gctttctttt tcgtctgatc gattgcacta gataattcct
     1321 ctaattgagt tttagcttct tcttgcttac caaagatctc ccctaaagca gtgacatttt
     1381 gctggaaaga ttcccatgga tttttcgcat ctaaagaaag gaaaatggtc ggagcaattt
     1441 gacttagatc tttttgaaaa tctctttgtc ggccagaaat gatgattaga tctggctgaa
     1501 gctgattaat tttttctaag tctggttctt ttattccacc tgctgattct acttttttat
     1561 aggatgaaag atactcagga aggctgcttg tagctgctcc aactacctta tctccaacac
     1621 caagagcatc catcgtatct aaagaaccat tgtcgaatac gacgactttt tctggattat
     1681 ttggcacctc gatttggtct ccattactat ctgttatcgt caatgtagtt tcagtttgtg
     1741 tgctgctttt ttttgtgtct gcctgactgg tgttgttgct atttccacat gctgctaaac
     1801 caaatgatgc tagaatcaag atacctacta ctaatttttt catctcttct ctcctaatgt
     1861 tagttttaga aataaagaca gaatctgcgg ccttcaatct cacaaatccg tatgtccatt
     1921 tcgtataatt catccaatac atcttttttt atcatttctt gggtcgttcc ttgcgtaaat
     1981 aactgtccat ctttcattgc aacaatttcg tctgcataac ttgcagcaaa gttgatatca
     2041 tgaagaacaa taacaattgt tttcccgatt tcttgtacta aattttttaa aatctgcatg
     2101 atttgaaccg caaaattcat atctagattg ttcaaaggtt catccagtaa aatataatcc
     2161 gtgtcttgcg ctagcaccat tgcaatatac gcacgttgga gttgaccgcc tgaaagagtg
     2221 tcaatcgatt gatccgctat ttcttctaac ccaaggtgtt ccagtgattc ctgaacgatt
     2281 tcctcgtctt ttgtcgtcaa ttttcctttc gtataaggaa atcgtccaaa ttctactaat
     2341 tctttcacgg tgatttggac atttatttca ttcgattgtc gtaggatcga taattttttg
     2401 gctaattcat tggatttcca agcctttact tcgttatggt ctaaatagat ttcacctgta
     2461 tcttttggta tcaaacgact gatcatcgac aacaccgttg actttccagc accatttgga
     2521 ccgataaaag cagtcaactt cttttcagga atcgttaact gtacatcttt aaccactgat
     2581 tttccatggt acagtttatt gaccttttgt aaatcaatca taggatactc ttcctttcgc
     2641 gaatcaattt tgtgacaaaa taaattcctc cgacaaattc aacgacgaca ctcactgttg
     2701 tattccagcc gaaaatctgc tctactgcga gctgtcctcc tgtcagaaac agcatagcta
     2761 ccagaaagct tccgataaat agctgaccat gacgataagt agaaaagaca cgataactga
     2821 tatttgcgac aataaaccct aaaaaagtaa ctggacccac aagagccgtt gatgtccctg
     2881 tcaaaagact gacacaacat aaaatcaaaa attcaaaacg atctgtgtct atcccgaggt
     2941 ttactgcttg ttcatttccg agatggaaaa cgtctagcac atgtcgatat cgaaataaca
     3001 ggtaccagca gccgataatg atcaaagaag caggcaaaag atactgactc tggatattgc
     3061 caaagcttgc aaataatctg ccttgaagca aatcatattc attcggatcc atgatgactt
     3121 gtaaaaaggt actgacacta ccaaaaagcg tacctaaaat catccctgac atcagtaata
     3181 aaaaaagatt cgctctcatt tttttcaaaa gaaagaaaaa taacaataga ctcagcccga
     3241 ccatcagccc aacgttcaat aagaaaaaaa ctgtacctgt ctgctgaagc atcgtcactc
     3301 ctccgataaa gaagaaaagg accgtttgga caaggacata taacgactca agtcccaata
     3361 ctgaaggagt caaaaactgg ttatgcgtaa ttgtctgaaa gctgattgtg gcactgctga
     3421 cagctagcga gacaaggata aatgcaaaaa ttttcttact cctcaatgca catgcaaaat
     3481 gccagtttcc atatgtatgg atcgtcaagt acattcctgt aacaaataaa acaagaacaa
     3541 gcataccaat aaaaatagat ggcttttttt taagcatatc gtcgtcccct tatcagaaga
     3601 taaaggaaga tagccccacc gatgattcca acgatcacac tgactggtat ttcataagga
     3661 aaaatcaaca cgcgagcgac aagatcgcaa aatactaaga acaaactacc tgctaaagca
     3721 gttacgccaa ttgttttgtg cacttgatct ccatatattc tagagacaag attcggaatg
     3781 atcactccga taaaaggaat gcttcctacc atgatcaaca tgactgcact agccaatgat
     3841 actaagccaa tgcctaaaaa ctgcatcttc tggtaatcta ttcccagatt tactgcaaac
     3901 gattcgccca gaccaatgat cgtaaatcgg taagcaaata gccaaaccaa gagcaacaat
     3961 ggaacaacag catacaataa ctcataattt cctcgtatca cagtcgcaaa attcccttga
     4021 agccatgaag tcatattttg taccaactgg aattgataag caaaaaaagt agcaactgaa
     4081 ccaatgatat tccctaacat gactccaatc aacggaagca tcaattgatt tttctggagg
     4141 aacaatcgcg taagggataa aaaaagcaat gttcctgcaa aagcaaagca aaatgctgtc
     4201 aaggatcgga caatcaagga tgcaccaggt aggaaaatca tagccactaa aatacctaaa
     4261 cgcgcactat ccatcgtgcc cgcagtcgtt ggtgaaacaa atttattttg ggtcaaatgt
     4321 tgcatgatca acccacaaat actactggtc gctcccacaa ggatcaaaca aatcgttcgt
     4381 ggcaaacgag tcactagcaa gatttgtaag ctttgcccat ctaactggag cagttctgac
     4441 aattgtacat tcgatactcc gatgaacaat gaaatactac ttaaaataac taataatgct
     4501 actagtcgtc cgtagcgcat ccaatccacc tttctaattg agaatgattt tcattcttat
     4561 tttaagtgaa tcgttgaaca aaagcaaccg ctttttcata tgttttaggt attattggga
     4621 atatgctata atcgtaagaa agacaaagag aagaaggagg gtatttttta tggcacaaga
     4681 tgatgtaaaa agccatttgg ataagggact ttatggtaca cctttagtga accctgaaga
     4741 acagcataaa tatttaggga cattcaggga acgctgctat cttagtatga ctgttgctga
     4801 aatgatccaa ccaaaaaaca aagaaaactt tttaaaagaa ctcaaaaaac atccagatgc
     4861 ttccatactc ttgaatgggg ctctacccct ttctgtccaa aaccaatata tccagctagc
     4921 aacgaaaaac aatagccgtt tcactgtagt caacgattac gtcactgcaa atcctgagga
     4981 attcggattg ctcttaacag caaaagaagc agtaaatgaa cctgtgattg atatcgaaaa
     5041 aaaataccca gaagaaaact cttcgccagc tgaaaaagaa aaaccaaaaa agaaaaaaag
     5101 cttctgggat cgtttatttt aaaacgaaaa aacacataaa tttttacatg caaaaaaagc
     5161 ttttgatcag attgacatca aataggacaa tctgtcaaaa gctttttctt ttaaaatact
     5221 tcaggatata acaatttcaa tacattcatc gtcaatcttc tgccttttcc tgcaaaagga
     5281 taaacatgca tatgcatggc tggattgaaa tttgcttcga tgatcccata tgctttttta
     5341 tcagttgtag gatcgatttc tttatctggt ataatcaaat cgataccact aatcttagct
     5401 cctagtgctt ctaccgcaga aacggcgatt tttttgtaag cttcagaaaa ttcatcagtc
     5461 atatcgatcg aatcaccacc tgtactgata tttgagttct cccttagata aacaatttga
     5521 ttggcttggg gaactgattc gatcgtcaac ccttgttctt tcaacatcaa ttgttctaat
     5581 tcacctaatt ggatcaattc taaaggtgca cgatgattcg ttccacgcaa agggtcacta
     5641 tttttttctt caacaagttc tttcactgtt cggatactgt caccaataac attagcaggg
     5701 actcgcaaca tgatggcttg gacttctccg tcgatcacaa agaaacgata ttccgttccc
     5761 ggcataaatt cttctactaa aaccgaactg tcttcacgaa aagcaatggc taaacctgct
     5821 tgataatctt ccagactagc tccttccttg aaaatcgtga tacctaaccc gtaattggtt
     5881 gatttaggtt tgatcacaaa tgcctgctcg gcaaatctcg gataagcttt taccgcttct
     5941 tccatggatg aaaattctgc gcctcctggt acccggaacc cagcttcttt taagactttt
     6001 ttcgttactg ttttgttttc catgataagt gggacgatat agctgtcctt acttgtcatg
     6061 tttgcatttt tcacatattc tacatggtct tggaattgca gacgcaaaaa ttgatccgac
     6121 tcatctaata ctttgacttg gacacctttt tggatggcat cgaacataaa tatctgagta
     6181 gaaagttcca tttcgcgaaa accggccaac tgatagggac gttcatggct ttctttataa
     6241 tattgatgtc ccaatgccac agcaacttct tgctgactcg atagctgtgc ttcttgatag
     6301 agtttcgctg ctaacgtttg ttcaggatga tccatttgag tcaatgcttc ctctactaaa
     6361 taaagagaat ccaaccaatc caattcaacc agcatttgct tcattccttc aagaatttct
     6421 ctaccttctt gatgaaattc tgtttgatct gatggttgtt ctagtgctac tttgttattt
     6481 cgagtcgtac cttctgctac ccattggtca cagtcttctt tttcttctgt ccataacatg
     6541 tacatcagga ataattgtaa gaaccgaact tgctctggat tgatacccaa tcttgcataa
     6601 ggattcaaat cgatattccg caattcaata taacgaacac ctgttgttgc taaatctgcg
     6661 actttctttc cgcctcggaa acgaagcggt gtataaaatt ctttctcctc cgaaagtttc
     6721 ccttcttcga tcattcgctc gatatctgct aaatactgtt tcatagaagc ataggaaact
     6781 ttcacatttg gatgattcgt atagcccagc gcactgttgc gaatactgcg aacaggttct
     6841 tgcggatgtg attcatctaa aaagtagttc ttctcactca ttggtgaagc accgaataaa
     6901 taagtgatca tccagcgata gcgcataaag tttctcgctg ttttcaaata aagttctgtt
     6961 ttgaattcgg aaaaatcttg aaattcttcc tgatggctga acaatgttct tagcaactca
     7021 tcgccaaatt caaaattgaa atgaataccg cttaccatct gttttcgctt cccgtattct
     7081 tttgctaaat aacgtcgata caagacatct tcgaaatttt ttaattttgc aataatgatc
     7141 tcttcgtctt tttccggtaa ggcaggtggc atacttaatg gccagatcat ctcttctttc
     7201 ggtatagaac gagcagtcac atcataaaca gcagcgagat actgaaataa ttcgggaata
     7261 gaatcggtaa caggggtgat catctctgtt tgtgtttcgc taaaatctgt ttggatatag
     7321 gggtgatagg atcgatcgcc aaagattgct ggatgatcgg tttttgctag atttcctgca
     7381 agatcaaccc gctgtccttc ccgttctata ccaaatcgag cttggtcgat aaaagggcgc
     7441 gcattgacat gcaataataa ttgcttaaaa ttcatcatta agtgacctcc aattcatatg
     7501 aatctaatta taatcaaaaa gtctaagaaa aagaacgaaa ttgtttgaga accgcttaca
     7561 aataagtata aaaatgaagg catcaaaaac acagctatgt taaaaacaat cattcaaaca
     7621 aggagctgca acttttgtca cagctcctgc tttttagaca tgggttattc ttcttttagt
     7681 aatcctttga acaatccaac gacaaaatca tttggatcaa acggctccaa gtcatcgatt
     7741 ccttcaccaa gaccgaccaa tttcactggt aaatgcaatt cattacggat agcaagtacg
     7801 ataccgccct ttgctgtacc atctaatttt gtcaaaacaa gaccggtcac atctgttgtt
     7861 tctttgaatt gttttgcttg aaccatcgcg ttttgaccag tggttgcatc taatacaaga
     7921 agtgtttctt ggggtgcttc tggtaattcc cgctggatta ctcgttttat tttttctaat
     7981 tcgttcatca agttcacttt attttgcagt cggcctgcag tatcaactag taaaacatct
     8041 gcctgctctg tctttgctct ttccaaagca tcgaatacga cagctgctgg atcgcctcct
     8101 gcattgccac gaacaacttc aacaccagcg cgttcgcccc agacaactaa ctggtcgatt
     8161 gctccagcgc ggaaggtatc tgctgctgcc atcaatactt ttttaccttc gtttttgaac
     8221 tgatgggcaa gtttaccaat acttgttgtt ttccctacac cattaacacc gacaaacaaa
     8281 ataactgtta gtccgtcttc ttggatattt agttcattta cttcattgat tccttccgct
     8341 tcatagagat ccacaagttt ctcgatgatc gcattttgga cttctgccga ttttttcgca
     8401 tttctgagtt tcacttcttg acgtaatgct tcggtgattc gtatcgctgt ttcaaaacct
     8461 acatctgcac cgataagtgt ttcttctaat tcttcaaaaa agtcttcatc gactgttcga
     8521 aaattggcaa acaactcatt catccgttca ccaaatgttt tacgcgtctt ttctaaaccc
     8581 ttgtcgtact tctcctgtac ttcaacttct tctgttttct cttcgccagt aaaagcacgc
     8641 ttgattttat caaaaaatcc catttgactc cccctactct aatacaaatt tttggatcac
     8701 tttggctacg ccattttctt cattgctggc tgtcacgaaa tctgctgctt ctttgacgaa
     8761 aggcacagca ttctccatcg ctacgcctag tccagcatat tggatcatcg gtaaatcatt
     8821 ttcctcatca ccgatcgcca tgatttcttc ttgtttcaaa ccaagatctt ttgccaataa
     8881 agaaatacca taagcttttg taacaccttt tggcataaat tccaaaagca tacttcttgt
     8941 tttgatgatc tcatacattt catggaattc tgctggtatt tctttgattt taggatctaa
     9001 ttcttcttgg tcataagcga ttactgcctt attaagcaca aattcatctg taatatcgct
     9061 tatatccgct ggttggaatt gcagcagctt attcaacaca ttgtacaagg attgcttcct
     9121 tggggcacta ggcagctgta aaaccacatt gtcagacaaa acatctagtg gcagatccaa
     9181 ttgctgagcc agtttgtata agcgttggat atctgatact tccatcactt tcttttccat
     9241 gatttcacca gtatcatttt tttgtaccaa accgccgttg aaagtgatgc tgtagtcacc
     9301 ttcatctacc agtcctaatt cttgcagata atgcgccatc gcagctaagg ggcgtcctgt
     9361 acatagaacg atcttgaccc cttgctgttt tgcttgtgct aatgcttgtt tattctcatc
     9421 tgaaatcagc ttttgttcat ttaataatgt accgtccaaa tcgattgcta ctaatttgat
     9481 cattttcgtt cctacacttt cgtttcgttg attttcgcct cttcattgat atcttccatc
     9541 cgtacagaaa cgatttttga tacaccggat tcctgcatcg tcactccgta cagtacatct
     9601 gctgcgacca tcgttccttt gcggtgtgtg actacgataa attgtgtgtc atcttggaaa
     9661 tcgctcaagt aacgaccaaa acgtttcacg ttggcttcat ctaacgctgc ttctacctca
     9721 tctaagatac aaaacggaac aggacagaca cggataatgg aaaagagcaa cgcgatggct
     9781 gtaagtgctc gttctcctcc agaaagcaaa cttagacttt gcagtttttt gccaggaggt
     9841 tgtacttcaa tctcaatacc tgttttcagc aaatcagttg gttccgtcag tactaattca
     9901 gctttaccgc cgccaaacat atttggaaag acaattttga attcttgacg gatagcttca
     9961 aatacttctt tgaatcttgt ccgcacttca tcatccatct catccatcgt ttcaaacaat
    10021 tggtttttcg ctgctaacag atcatctcgc tgtgttgcta agaatgtatg tctttcactg
    10081 acttgttcgt attgttcgat tgaatttaag tttactggtc ccagtttttc aatctgttct
    10141 tttaaggatg aaacctttgt tctactgtct tcgatatcgg ttgtttcttg atagtcttgg
    10201 gaagctttct caaatgtcag ctgatattct gactgcagat aggtcaaatg attatctaaa
    10261 atcatttcag ctcggtcttt ttggacttct aatttggatt gttccgacag tcgtgctttt
    10321 tgctcacgat tacgctctgc caagactgct tcaagcttat cgatctcctt ttgccttcta
    10381 tctcgctgct ctttggcttt tgccaactcg gcttttaatg tttctcgctg agcagataat
    10441 tcgttaattt gtttttccaa actttcttct gtgacttcat gatcagaaaa gtctgctgtc
    10501 aatgtcgcca actgcttttc tatcgcttct tgacgttcgg ttgcctctgc tttttggact
    10561 cgggcaccgc ggagctggat ctgcagatga ttaaactgct ctttcaagac agcaagatct
    10621 gcttgttctt gtgctttttg actttgtact tgtgctcttc tggcttccat ctggtcgctt
    10681 tcttgactta acgatttgat ttcttcgtcg attttttgtc gttgcgattc aagatctgtt
    10741 tgtttgtctt tcaattcgtt cgtttgcttt tcgtaatctt cgataaactg ctgcagttca
    10801 cgtgtctcaa aagttgagat ttgcttttct ttttcaaaac gttcaagttc atttatgatg
    10861 ttttgtagct gatttgtcgc ttcttgttct tcaaaacgta attgttcgcc tcgtgtacgc
    10921 aaaacttctt gttcttcggc taaacgagct gtttcttgtt gcagctcctg aacttttttt
    10981 tcttgtgctt ggagttgttt atctgcttct tcaaattcac tagtcaactg tttcaattct
    11041 tgattttgta caaacaaact gccttggttt cctcgtttat tcgctccacc tgtcatggat
    11101 cctccggcat tcatcacatc accttctaag gaaaccactc gatattgata gcggatcgtt
    11161 tgcgcgattg cattggcact agttaagtct ttggctaaaa ggatcgttcc tagcaggttt
    11221 tgaaccacag tttggatttg atcgggataa ctgacttgtt cactggcaat ccctataaac
    11281 ccttctacag ctgctgcttg tgtcaatata tgagccggta attgtcttgg tttgattgtc
    11341 gtcagtggaa ggaatgttgc tcttcctcct ctttgctgtt tcaaatacgt gatggcttgc
    11401 cgagcatctt tttcattttc aacgatgaca tgctgggctg ccccacctaa agctgcttcg
    11461 atagctaacg tgaaatctgc aggaacatcg atcagttcag ccactgctcc gacaataccc
    11521 gaaagctgct gtttatgctg caagaccaat cgaacccctt ggtaaaaacc aaaatagttt
    11581 tcttggattt cttgaaggct tttcttacga gcacgtagct gctggacttg attcattaat
    11641 tgatacattt taggctgttc ttttgctaat gcttcttgga actttttccc gttttgttgg
    11701 atcatttcta acttctcttt tgtttcagtt aaagatgatt ttagctgcgc ctgcttttgt
    11761 gtcaattctt ctttttgtaa gatcaatcgg tctactgatg cttctacttc cgattgcttg
    11821 gctaacgtct gcttgctttt tgctgtttct tgtatatatt gacgttctag atacttcagt
    11881 tcgtttccta cagctgcttg ttcttgcatc aaatcaacat attgatctct taattctgcc
    11941 aataattctt tcgttgattt ttggtatttt tctaattctt gctgtgtttt tatgagattt
    12001 gcttcagctt tttggatctc tgtttctttt tctgctgctg ctttcatcaa gctttcttga
    12061 agcttttcaa aatgtttaac ctttttttgt gcttctgcta atgaagtttg atattcttgc
    12121 gagctttttt gtgtatgctt tgtccgctcc tgcaaaacat ctttttgtcc ttctgtctgc
    12181 ttcagttttt ccgaaagatc taataatact tgctgatttt tttcgatcag acgatcggct
    12241 tgcgcatttt ctttacgctg cttcgccaaa atggattctt gttcttggat cgactcagat
    12301 agtttgccta attctagatt gaatttcgca agctgtgctt gtttgttgtc ccaatctttt
    12361 tttgctgtct tgatttcagc aaccatcaaa gagacatcgg tttgagtcag tgtttctttt
    12421 agccgtaaaa actcctttgc tgcctcactc tgggcggcca aaggagttaa ctgttcttcc
    12481 aattcgtgga tgatgtcttg gacgcggctg aggttgtctt ctgtttcaaa aagcttctgt
    12541 tctgctttct ttttccgctg cttgtatttc aagacaccag ctgcttcttc aaagatccca
    12601 cgacggtctt ccggtttact actgaaaatc gcttctactt ttccttgaga aatgattgag
    12661 aacgattctt ttcctaatcc agaatctaag aacaattctt ggatatcttt taatctgcag
    12721 ctttgtttat tgatgaaaaa ctcgctttcc ccagtccgtc gataacggcg ggttacactg
    12781 atctcattgt actccaatgg cagataatga tcactattgt ctaaaatcac tgtgacttct
    12841 gcaatattca gctgcttacg ggtatcggaa ccggcaaaga tgatatccgg catttttccg
    12901 cctcgcagac ttttagcaga ttgttctcct aatacccagc ggatagcctc tgtaatattg
    12961 ctttttccgc tgccatttgg tccaaccact gctgtgacgc tattttcaaa atcaatgact
    13021 gtcttgtctg caaatgattt aaaaccggct atttcaattc gtttcaaata caaagctttc
    13081 cctcacttac ttttacagtg ctgccaatgc attttccgcc gcagcttgtt ctgctaattt
    13141 tttcgatttc ccttgtccag tgccaatcaa ttgatcatct acataaactt cgatccaaaa
    13201 tactcgttca tgagctggac cttcttcatt gatcaatcgg tattcgattg atacatcacc
    13261 tgatttttgc aagacttctt gtagttttgt tttatgatcc atctcatgtg aaaaagcacc
    13321 tgctttgact ttaggaaaaa tcactttttc gatgaatgca tgggtcgtgt cgaatccctg
    13381 gtcaagatat aacgcgccta aaaaagcttc gaataggtcg caaagcaatg ctggtcgcgt
    13441 acgtcctcct gaattctctt ctcccttgcc caacaaaaca tattgatcaa agtgacattc
    13501 tttggcaaat ttcgacaagc tgtcttcgcg aacgatcgca gctcgtgttt ttgtcaattt
    13561 tccttctggc aaatgaggat ataaacggaa taaatactga gaaaccatca gttctaaaac
    13621 ggcatctcct aaaaattcaa gtcgctcatt atccgataat tgcagattgc gatgctcatt
    13681 cacataggat gaatgggtaa aagcttgttc aagcaaattt agatcgtgaa aaacgatgtt
    13741 atagttttct tttagttctt ttgtcagctg gttgtccatt ttccacattc cttttatgga
    13801 tattttttcg atacttcctt attatactgg ttttttgtca aatttgaaat ggttcggtca
    13861 aaaggtaagt aaagctttaa taatcctagt gattgacgag aagttgtgaa aaattcgttt
    13921 agtttcgagt atgaagaaaa aattttgtga aaatggcttt ccatattttt gcataaattt
    13981 gacttaatac cgagaaactg gactttgaac accgtttatt ggagaagtca tgaaatcagc
    14041 gccttgacct gaatattaga gaaataaaca gaaaaatatc aaaaaaccga aaaaggacat
    14101 cgcccttttt cggtcatttt tagtgattat gaacgtgaat ctgctgtaac agaaacattt
    14161 gcccatgggt ttggtacatc ataagaccaa tcccagtctt tgacacggtc gttgactggc
    14221 aatacttcat tacggaacaa cgttggaatc gcaaatgctt cttcaaatgc atattgttgc
    14281 catttttgat atgcttcttt ttggtactct tcatcaaatg ctttgcttga actgatatcg
    14341 ttgatcaatt ttgtattttc ttctgattca taacgagtgt agttgaacgc agcatttggt
    14401 ccccatagtc cacttggatt tggatcagtt cctgtattcc aagcagcttg gtaaacatca
    14461 atttcaggat catcgttttc taatttatca tagaaagaat tgaattcgat cagacgacct
    14521 gttgtgtatt ggacatccaa accaatttct ttccattgtt ggatatagta atctgccaat
    14581 ggttgcgctg tttccccacc ggacatcgaa gcaaagttga ttgttagttt atttccatct
    14641 ttgtcttctc ttaaaccatc gtcatccaca tctttgtagc cagcatcatc taatagtttt
    14701 ttcgctttgt ctagatcttg tgtgaatcct ttcatctcat catcatggta tgactggaat
    14761 actggaggaa tcaatgaagt cgcattgctt ctcaaacctg catagaaacg ttcacctact
    14821 gcattattat ctaatgcata gcccattgct tgacgtaatg atttatcagc catttttgaa
    14881 tctggattgt attccacact ctttgtatct gcattccatt tacctaactt gaatcctaga
    14941 taagtataag aagtttgttc acggcctaag tttgtatagc cagcaatttc tttataggta
    15001 tcgtaagtat ctgttggcat ttggaaaatc atatcatata atttagagtt caatgcttca
    15061 gttgcacttg ccatcggtac agatttcatc gtgatctttt tcaatttagg cgtaccattc
    15121 cagtagtgct cgtttggcaa gtattctact gattctcctg caacgatatt actcatgtaa
    15181 taagggccaa aagtcactgg atttttacgt acttgatcgc ttgattccat atctttgatt
    15241 gctacacctt ctaacgtatg ttttggtgct gcatatgacc agataccacc acccgattga
    15301 agcatgcttg ggtttacttc tttatattcg atttcaactg ttttgtcgtc tatttttttg
    15361 attccggaga ttgtgtcggc acttccatca tgatattctt ccatacctac gatgttacgg
    15421 aaagtgtcgt cataacggat accagtgtaa tctttattcc caatgatttc gtaagggaaa
    15481 atcacatcgt ccgccgttac atcttcacca tctgaccatt tgacgttgtc ttttactttg
    15541 attgtcgctt ttttcgtatc ttcatctaga tcaagcgttg cagctccgtc gtctgtgatt
    15601 ctgaaatcgt catcgtttgc aaatagcggt tcatgagaag gagccataaa agctgcatca
    15661 tatgaatctt ggtaaaactc ccactggaac aatcctttaa actgactgtc cgttgcgacc
    15721 gcagcttcta attcgccgtc ttcgattgca tcatcattat tttttgttgc aattggaaaa
    15781 gttaattcct ctttgttatt gccgttgcct ccagagttgt ttccagaggt atctgtacct
    15841 ccgcccccgc atgctgcaag aacgacagca gacatcagcg ctgcaaatcc aaaaatcgtt
    15901 ttcttcattc ctatttcccc cttcgcttct atactagcct aaccgttggc gcgcatctgc
    15961 agaacgcttg aatgcttgtc caacataatt gatactcaac atcaaaacta aaataaggat
    16021 tgatgcgggt aaccagatcc attgccgatt cactaagact tctccgttac ttgcataacc
    16081 gatcaatgta cctaaactag gtacatttgc cggcaagccg aaacctaaga aagtcaaagt
    16141 tgtctcgata ccgatgtttg ccgcaaagtt catcgtcaaa ttagtaatga ttaatgaact
    16201 tagattcggc atgatttcgc ggaacatgat tttaaaatca ttggttccca ttgtttttga
    16261 ggcactgaca taatctcttc ttgcttcaga taatgtcttg cttcggaata atcgggcttt
    16321 agctacccaa taaaatgcac tcatgatgaa tataaaggac cagacattgt atctggaaat
    16381 aatcgatacg aagacgataa tgatcagcat gatcggtaga atcatgataa aatcaacgat
    16441 ccgcatcaag atgttgtcaa tgatccctcc gtagtagcca gagatgatcc ctaatccaac
    16501 cccgatgatc gaagtaagga tcgtgatcgc aaatccgatc gtaacagagt ttctagcacc
    16561 tatgatcaat tgtcctaata catcacgtcc gccttcatct gctcccagaa tgaacttttg
    16621 tccttgtgtc gaaactgttc ctggtgcagc gtatttgtcg aagatactaa catacatcac
    16681 tttgtcttga tcggtcagca tcgcaccgat aaagatcact aataataaaa ccactagcaa
    16741 acctaatgaa aaaatagcaa ttttatcttt tttaaattct cgagcaatca ttcgtatccc
    16801 cataggagga atactttctt gaactgctgt attgtttttt tcactcatcg atccattctc
    16861 ctttctgatt ggttttattg aatccgaata cgcgggtcaa cgatactcat gatgatatct
    16921 gaaagtaatg tccccactag cgtcgcgatt cctaaaatca atactaagga tgtgatcacg
    16981 gtatagtccc gttggccgat ggaatcaata aatagttttc cgactcccgg ataaccaaaa
    17041 atcttttcga taactacaga accagcaatc aacgaagtga tttcataccc catttgagaa
    17101 gccatcggca acgctgcatt tcggaaaata tgtcgactga atactttatt cgtcggtacc
    17161 cctttggaac gggctgttct gacaaaatcg agcgatttcg aatcgattac ttcacttcgc
    17221 agatattgga ttgtgacggc agtcgccaaa aatgcttggg taattgatgg taacagaata
    17281 tatttcaaac ggtctatcca ttgagtgata aatccacctt gcaaacctag tgtttgtgat
    17341 ccacttgttg ggaaccagcc taaacgataa ccaaaaacaa atagcatgat caagccgaag
    17401 ataaataaag gaacagcaaa actaaagaag ttgtagatta cgacaatctt atccaagatt
    17461 gaattttgat accggccagc aagcatcccc agaggcacag caatcaaata agtaaggaca
    17521 actgtcaaga tcgatagata taaagtgttg atgatccgtt cgccgatcaa gtcagcaaca
    17581 gagcgtttgt ataagaaact ttgaccaaaa tcgccttgca aagcatttcc gatccaacgg
    17641 aagtactgga cataccatgg gtcattcaag ccagctgctt gtcgcatctg ttcgatcact
    17701 gccggatcct gatttggatt gatcaaaccg gtaaatggat cccccggcat cgcttgggct
    17761 aataagaaga tcagaacact taaaataata acttgcggga tcatcaataa aacacgtcgc
    17821 agaatcgttt tccacattag cttgcacctc catctttcaa cgccactttg tgtgtagttg
    17881 tgatcgtacg caaatcgtag actcgaccag tcgcatcata gtat
//
LOCUS       DACDHJ010000038        17508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDHJ010000038 DACDHJ010000000
VERSION     DACDHJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.039"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            112..1797
                     /locus_tag="IV223_001008"
     CDS             112..1797
                     /locus_tag="IV223_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8711312.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1811..3094
                     /locus_tag="IV223_001009"
     CDS             1811..3094
                     /locus_tag="IV223_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8711313.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3164..4033
                     /locus_tag="IV223_001010"
     CDS             3164..4033
                     /locus_tag="IV223_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8711314.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4030..4866
                     /locus_tag="IV223_001011"
     CDS             4030..4866
                     /locus_tag="IV223_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8711315.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4892..5959
                     /locus_tag="IV223_001012"
     CDS             4892..5959
                     /locus_tag="IV223_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8711316.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5956..6783
                     /locus_tag="IV223_001013"
     CDS             5956..6783
                     /locus_tag="IV223_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8711317.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6828..7853
                     /locus_tag="IV223_001014"
     CDS             6828..7853
                     /locus_tag="IV223_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8711318.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7850..10033
                     /locus_tag="IV223_001015"
     CDS             7850..10033
                     /locus_tag="IV223_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8711319.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10033..11163
                     /locus_tag="IV223_001016"
     CDS             10033..11163
                     /locus_tag="IV223_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8711320.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11160..11837
                     /gene="pgmB"
                     /locus_tag="IV223_001017"
     CDS             11160..11837
                     /gene="pgmB"
                     /locus_tag="IV223_001017"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8711321.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11921..12448
                     /gene="msrA"
                     /locus_tag="IV223_001018"
     CDS             11921..12448
                     /gene="msrA"
                     /locus_tag="IV223_001018"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8711322.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12649..13335)
                     /locus_tag="IV223_001019"
     CDS             complement(12649..13335)
                     /locus_tag="IV223_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8711323.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13415..14953
                     /locus_tag="IV223_001020"
     CDS             13415..14953
                     /locus_tag="IV223_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8711324.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15073..15540
                     /locus_tag="IV223_001021"
     CDS             15073..15540
                     /locus_tag="IV223_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8711325.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16059..16502
                     /gene="rplM"
                     /locus_tag="IV223_001022"
     CDS             16059..16502
                     /gene="rplM"
                     /locus_tag="IV223_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8711326.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16518..16910
                     /gene="rpsI"
                     /locus_tag="IV223_001023"
     CDS             16518..16910
                     /gene="rpsI"
                     /locus_tag="IV223_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8711327.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16998..>17508)
                     /locus_tag="IV223_001024"
     CDS             complement(16998..>17508)
                     /locus_tag="IV223_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8711328.1"
                     /translation="YALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVV
                     MTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRH
                     PDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEA
                     FQRGLNKCD"
BASE COUNT         5699 a         3215 c         3776 g         4818 t
ORIGIN      
        1 ttgtattttt gtgtatatca aaattattta aaaaaatgct attgacagta agcgttttcc
       61 ataatatgat caaatcgttg ggaacgctcc caaaaaagaa aggggaatcc gatgtataca
      121 aaacttttac aagcgattta tcgcgactca gatttgtctg agaaaaccgc ctctatgatt
      181 gaacaaaaag tcagtgttta tcagcagaag aaaggccagg caggcaaact gcattgggac
      241 gaagaagata ttttcttgat tacttatggg gatcaatttt ttgaagagca gcaaccaact
      301 ttgcgtacat ttaagaaatt ttatcaaaag tatttggcag atacatttag agttgtacat
      361 ttcttgccat ttttcccata tacatctgat gatggtttct ccgttgtaaa ttatcaggca
      421 atcaacgacc gtttgggcac atgggaagat atagaagaaa tgagaagcga aacacggtta
      481 atgttcgact ttgtctgcaa tcatatgtct gccgaaagta cttggtttca ggaatattta
      541 aacttgaatg atacgtacaa agattttttt attgatttgc cactggatac agatacaagc
      601 agcgtgacta gaccaaggac cagcccgtta cttacaacgt tcattgacaa aaacgggaaa
      661 gaaaaacacg tatggacgac ttttagcgaa gatcaagtag acttgaattt taaaaatcca
      721 gaagttttat tgaaaatgat cgatattctt ttgttttact tagaaaaagg cgcttcattt
      781 attcgcttgg acgctgtggg atttttatgg aaagaactag gaacttcttg tatacacctg
      841 ccagaaacgc ataaaatcat ccaattattc cgtgcaatcg ctgatgaagc agctccagga
      901 acggtattga tcactgagac aaatgtgcca cacaaagata atatcagtta ttttgggaat
      961 ggcgagaatg aagcacagat ggtctatcag ttttcgctac cacctttgac ccttcaagcg
     1021 attcgttcgg gtaatacaag taagttgaaa gaatggttaa aaaccatcga tttcgcatcg
     1081 aaaaaaacaa ctttttttaa ttttttagcc tcccatgacg gtatcggatt gaatccggta
     1141 cgaggtattc tgccggaaaa aacgatcctt gaattagtgg aacagctgaa gaaagagggt
     1201 acccgagtaa actacaagaa aaacccagat ggaacgacca gtccatatga gataaacgct
     1261 acgtatctag atgcgttaag ttctccaaca gatacgacgg aatggagaag caaacgcttt
     1321 ttagctgctc attcattttt ggtaacgtta ccaggcgtgc cggcaattta tattcaaagt
     1381 gtgctgggaa gtcgaaatga ttacaaaggc gtagaaaaaa caggagagaa tcgaaaaatc
     1441 aatcggcaaa aatttttgtt agcagatatt gagaaagagt tgcagacaga tccattaaga
     1501 acagcagtat atggtgaatt gacccagatg ctatctattc gaaaaaaaga aaaagccttc
     1561 catccgaata gtttgattta cttactggac tcagcagatt cttttatcag ttttattcgc
     1621 gaagcgaagg aagagaaagt cttagttatc cataattttt ccaatcaatc agcaggttat
     1681 cttttaccaa aaggagatta cctagacctg ttttctggac aaattatcca ttgcagtcaa
     1741 gaacgaaagt tgatattcca gccgtatgaa tatatgtggc tgaaaataat aaaataagag
     1801 gtggaaaaaa atgaaaaaaa ttattgttac tacctgtctg ctagctactg gatttcttct
     1861 tactgcttgt ggtgaaagtc aaagctctaa cgaatcacaa cagacaacga ttcaattcat
     1921 gcactcttct gtcgaacagg aacgattaag tgtgatcaat gaactggttg ctgattttga
     1981 aaaagaaaat ccagacatca aaatcaagca agtgccggta gaagaatcag cgttcaatac
     2041 aaaaatcgtt acgttagctc gttccggtaa attaccagaa gtaatggaag tcagtcagga
     2101 ttttgctaaa gtgatggata aagatgaact gattgatcag acggcagtcc aaaacgttat
     2161 cgaggaagct ggagaagata attattatga cggagccaaa aaccttgttc gttcagaaga
     2221 tggaaaaagc tatattgctg caccaattag cggctgggtt caaggtattt ggtatgacaa
     2281 agaaaaatta tcagaagctg gtttttcaga accagaaaat tgggatgata ttttaaaaat
     2341 tgctcaacat ttcacagata aagagaataa acaatatgga attgctatgc ctactgctga
     2401 aggaacaatg agtgagcaag cgttctctca atttgctttg tcaaatggag caaatgtttt
     2461 ggatgatgaa ggcgaggtaa cgatcgatac ggaaaagatg agagaagcat tgtcgtttta
     2521 tcaaaactta tcccaataca cgatgccagg ctctaatgat gtgacagaaa tcaaagatgc
     2581 tttcatgaat ggaacagcac caatggcaat ctattcaact tacattttgc cttctgttta
     2641 tgaagcgggg aactcagaaa atatcggttt tgctatccca acacaaaaag atcaagctgt
     2701 ctatggcaca gtatctggat tgacgatttc ttctggtcta gacgagaaac aaaaagaggc
     2761 tgctgaagca ttcacagctt tcttatctca accgaaaaac atggaaaagt gggtcttgat
     2821 gtctcctggt ggtgctcaac cagtgaataa acaagtagtc gaactggatg gttacaaaga
     2881 aaatgaagtc atcaagtctt ttggcgagct gccatctgag attgctagtg cttttgatga
     2941 agtacaagtc tttggactag tcggggaaaa gaatttcacg aaaatgggag atatcacaag
     3001 ctcaggtgtg atcggaaaag cagtgaacca agtaactgtc ggaaacgaag atgtcgatca
     3061 agctttagct gacgcacaaa aaaatacgaa ataacaagaa gttctgccaa tggtgttcga
     3121 aaaaagtaga tagctattgg cagattatca gggggaggtt tcaatgaatc gtccaaggaa
     3181 acattctgac ttaaaattag ccatgggact tttagcacca agcatgcttt tattagctgt
     3241 tttagtgctg attccaatgc tttccaatat tcagatcagt tttttcgata tgccgctgaa
     3301 tcctaactca gacgccgttt ttatcggatt aagcaattat cttgaaattt taagtgatag
     3361 tgaattttat cgttctttag gtttaacagt tgcttacaca ttcttagttg tagcaggcag
     3421 tacagcttta ggattagccg tagctatttt gcttaaccgc aagttcagat ttaggaaaac
     3481 ggtgcgttca ctgattttat taagttatgt tacgccatct atttctttga tttttgcttg
     3541 gaaatatatg ttcaataata gttatggaat cgtcaatgat ctgttagtag ataggttgca
     3601 gcttttcaac gaagcaccgc tgtggttcga ccagccagtt gctagcttct ttatggtcgt
     3661 tttgtttgct atctggagat actatccgta tgcgtttatt tcatttttgg ctattttgca
     3721 aacagtcgac aaaacgcttt atgaagcagc tgaaatggac ggtgcaaatg cttggcagaa
     3781 gttcaagatc gtgacactgc cggcaatcaa accagtacta gcaactgtga ttgttttacg
     3841 atctatctgg atgttctata tgtttacaga tgtctatcta ctgacgaata aagtcaatat
     3901 cttaggcgtt tatttgtatc agacagcctt tgcgttcaat gatcttggaa aagcagcagc
     3961 tatatcgatt ctattgttca tcattattgc agcggtcgtc ttgctctcac gaaagaaggt
     4021 ggacttgaaa tgaaaaataa aaagagcagg aaaatcttat tttacgcgac gattttgttg
     4081 ctattagctg tcaatttgtt tccatttttg atcatgctga tgacatcatt caaggattca
     4141 caagaagcaa ttgcaacgac acaaactatt tttccgaaag aatggacttt tgaacattat
     4201 caggatattt tcaatgctga gatttttcca ttcatcatgt atttcaaaaa tagtttagtg
     4261 atctccttga tagcctccgt aactgcagta gtgattggga tcagcggggc atacgctttg
     4321 tcaaaactac gcttcaaagc tcgctcgaaa atcaatgcta gcttttacac cgtttatatg
     4381 ttttccggta ttttgatcgt tgttccatta tttaaaatca tttccggtct aggtttgtat
     4441 gataccaata cagcactgat tatcacgatg gtcgttcaaa cacttcctac tgcgatattc
     4501 atgctaaaaa gttattttga cacgattccg actgatattg aagaagcagc aatgatcgat
     4561 ggtcttagcc ggatccagat cattattcgg atcattatac cattggctat ttctggaatc
     4621 atttctgtct tcgtttattg ctttatggtt tcgtggaatg attatttatt tgcttcgatt
     4681 ttcctgtcga cctccgaaaa atttacttta ccaatcggtt tgaacacatt attcagtaca
     4741 ccagactaca tttggggacg aatgatggcc gcttcattgg tgaccgcttt accagtcatc
     4801 attatgtatg cgttatctga acgatttatt aaaggtgatt tagtcgaagg cggcgtaaaa
     4861 ggataaaaag aaaagaaaga ggaaagaaaa gatgaaaaaa ttagttgcat tagaaccaag
     4921 aatagccgga ttcaaagatt acgaagaccg tgagatcgaa aagaacgaag tacgtatccg
     4981 agtaattttc gctactccaa aaccaggaac agaaattgtc gactttagag gattaagtcc
     5041 atttatcgat gaagaatttt cagaagaatg gcgcctattc ttgccaagag aagaaggaag
     5101 ccaacgcggt gtggtatttg gcgaatttca attaggaaat atgattgtcg gtacgatcga
     5161 agaaacgggc agtttggtga cagagtatca aaaaggagag ttagtctgca cttatggacc
     5221 cattcgtgaa acgctgatta caaacggtgt tgataattat aaactacgta aacttgatcg
     5281 gttggatcaa tggaaaaatg cagtatgtta cgatcctgct caattcgctt taggtggtat
     5341 ccgggaagca catgttcgag caggtgatgc tgtagcaatc ttcggtttag gcgctatcgg
     5401 gcaaattgcg attcaattag ctaaaaaagc aggtgctact accatcgttg ggatcgatcc
     5461 catagcgcag cgtagagaat tagcagaaaa atacggtgcc gattacacgc tagatcctat
     5521 gaaagcagat gtcggttttc gtctaaagga attgacagat aaacgtggag tagattcagt
     5581 tattgaaacg agtggcagtg ctgccgcatt acaagcttct ttaagaggga tcggctatgg
     5641 cgggatcatt tcttatgttg cctttgctaa accttttcca gctgggttta atcttggtcg
     5701 cgaagcacat ttcaataatg caaaaatcgt ctttgctcga gcatctagtg aaccaaaccc
     5761 agattaccca agatggaacc gtaaacgaat cgaggacaca tgttgggaat tattgatgaa
     5821 cggttacttg gattgtgaag aaatcattga tccagttgtg ccatttgcag aaagcgcaga
     5881 agcttacatg cgttatgtag accagcaccc agaagaaagt atcaaaatgg gcgttcaagt
     5941 ccgaggagga gaacaatgaa attcggtacg caagatcaag catttttccc aagagacatt
     6001 ttagaaaaat ttagatttat caaagactgt ggatttgaag aatacgaaat cgacggtaag
     6061 ttgttaatag aaaatatcga cacagtcaaa caggcaatca aagaaacagg gatcccagta
     6121 gtgacagctt gtaacggcta cgatgggtgg atcggtgact ttatcgaaga acggcgtctt
     6181 aatggtgtac gtcaaattgc tgagattttg cgtgcactaa gcgaggttgg aggcaaagga
     6241 atcgttatcc cagcagcttg gggaatgttc acttatcgat taccgccgat ggtttcaccc
     6301 cgaagcaaag aaggagacta taaagcagtc agcgaatcac ttcgttattt agataaagta
     6361 gccgaagaaa caggtacggt tatttatttg gagcctttga accgatacca agaccatatg
     6421 atcaatttgt tagcggatgc gcgtcattac attgactcta atcaattgaa acatgtgcag
     6481 atcatcgctg atttttatca tatgaatatt gaagaagatg atatttcaaa agcattgact
     6541 acgcaaaaag agtatgtggg tcatatccat ttagcagata atcatcgcta ccaaccagga
     6601 tctggcgcaa ttgattttac tcgccatttt caaacgctac ttcaaaatga gtatgaaggt
     6661 tatatggtat atgaatgtcg tttgcgagga gaaaatgaag tgaaaattta tcaggaatca
     6721 ttgcaatact tgaaaaactg tctggaaaca gcgcgtcatt taaatgattt agagaaagaa
     6781 tagagaaaga ataacaataa gtaaataagc aaaggaggga gaaataaatg aagaaagtag
     6841 caatcatagg cgctggacaa gtagcagaaa aagtacatgc tgcttattat caaaaaagat
     6901 cagatgtctt agaattggcc gctgtggttg atccagtcag ccagcgagca gaagactttt
     6961 gccgacgtac aggattttct catgcgtata catcagttac acaaatgctg aatgagattt
     7021 ctcccgatat cgtgagtgtc tgtacaccca atcgctttca cttcgaacat gtattggctg
     7081 ccctcgaagc taatgcttca gtgatgtgcg agaaaccacc agctatgaca gctgatcaag
     7141 caagaaagat ggcagagaca gccgataaaa acgggcagat tttggcttat ggattccagc
     7201 atcgtttttc tgacgaagca acaatgatca agaatcatct tgatgttctc ggggatattt
     7261 attacgtaga agcgaatgcg cttcgtcggt ctggtgttcc aggctgggga acgtttatcg
     7321 acaaagaact tcaaggaggc gggcctttga tcgattatgg cattcacatg cttgatacag
     7381 cgatgtatat tactggattt cctaaagtaa aaagcgttcg ggcgtacagc ttccaaaaaa
     7441 ttggtacgaa aaaaaattca gggaaattcg ggaaatggga tccaagcaaa ttcacagtag
     7501 aagatgcaat ttttggcaca ctggaattag aaaacggcaa gatcatccaa ctgaatactt
     7561 cgtttgcttt gaatatcaga gaagacaaac tgaatgtggc tttttgtggc gatcaggcag
     7621 gtgctaatct gtatccaggc gaactattcg aagatgtaga tggaaagctg gtttccttac
     7681 aaaagaccac gccagttaat gacccacatg aactaagtat ggctcatttt gtgaatcggg
     7741 cgctcggtga ccagactcag atgattgcag atggctggca aggatatgtc atccagaatc
     7801 tggtggaaaa tttatatcgt tcggcagaaa caggagaagt tattacctta tgaaagtaga
     7861 agaaaaacag tttgatttag agtatatcaa taagtatgcg agtcagatga ccattggaaa
     7921 tggatttttt ggtatcagag gaagccagga ggaagactat cggaatcaag taaggggaat
     7981 gtttgcagct ggtgtatata accgaccttt aggagcagaa tcagccgagt tagtgaatct
     8041 cccagatgtc atcagatggg aaataaaaat cgacggtgaa catttttctc ttgagacgat
     8101 gaaagttctt gcatacgagc gggcactaga tttagaaatg ggagagctta aacgctatgc
     8161 gctcattgaa actgctcaag gaaaaaaagt ggaagtcatt acttgtcgtg ttgctgatca
     8221 aaataatctc catcaggcag ccctgaaagt ggtcatcaag ccgttgaccg aagactgcca
     8281 agtagatgtg aaaacgggta tcgatggaca gcaaaccaat tttggtacac agcagttggt
     8341 tgaaaaagaa ctgcgagtat ttggtgacga tttgatcagt gcatcctacc agacaacaga
     8401 aagtaaaatt gatattggga ttgcagctaa attcaataaa gcaggtgtat tccaagcaaa
     8461 aaatcgccgg atcaccgctt ccttttcaga aaagatcaga aaaaatacgt cctttacgct
     8521 ggagaagatc atcgtgctct ctacctctct tcagcaaaca gacccgttta agaaaagtat
     8581 tgcccaagcc cagaaaagcg ttagttatac agaaatttta gagcagtcga tgtcttattg
     8641 gacagatttt tggcatcagc atcggataac gatccaaggc gattcttctt tcgatcagtt
     8701 agcgatggac ttcgctgctt accatttaca gattatgact ccccgcaatg atccaagtgt
     8761 tagtgtaggg gcgaagggac taactggcga aggttataaa gggcatgttt tctgggatac
     8821 ggaaattttt atcctgcctt tctttttata tcagtttcct gaagtagcaa agaatttgct
     8881 gctgtaccgt tatgatcgat tagaaggcgc acggcaaaaa gcaagagatt atcactatca
     8941 aggtgcactt ttcccatggg aaagcgcaca cactggtcag gaagagacac ccaaatttgc
     9001 tgcattgaat atcaaaaccg gaacaagaca agaagttgct tcagctgccg cagaacatca
     9061 tatcagtgca gaccttgctt atgccgttca agagtatgtc caagcaacag aggatcaatc
     9121 gtttttagaa acaaagggaa aaacgctcat tcatgaaaca gcagcttttt ggctgagcag
     9181 agctgtaaaa gtcgatgatc atttagagat acacagagtc attggtccag atgaatacac
     9241 agaatatatc gacaataatg catatacgaa ctatctggcg cattataatg tggccttagc
     9301 tgttaaatat caagctggcg atccgctttt tttgaaagaa tgtcaaaatt ttttagataa
     9361 gttatatttg ccagtgccta acaaagaaca gctgattccg caagatgaca cgtatctcag
     9421 taagccagtt attgatttga ccacttacaa agaacgtcct ggcagccagt ctattttact
     9481 tgattatgcc cgtagcgaag taaatgaact ccaagtcagc aagcaagcag atcttgtcat
     9541 gctgttatac ttattacctg agtatttttc tcaaccaatc gtttctaaga atttgcatta
     9601 ctatgaaaac aaaacaatcc acgactcatc gttgagtaaa gcgattcacg ccatagaggc
     9661 ttgccgtatc caagaaatgg aatgggcgta tcatttattc caagaagcgt gcctgattga
     9721 tttaggacca gatccgcatt catcagatga cggaattcat gctgcttcat taggagcact
     9781 atggctagca gttatatttg gtttttcagg tattacagta acgaatggaa ctctttctat
     9841 ttctccaaga cttccttcag catgggaaaa aatttctttt ccttttattt ggaaaaatca
     9901 caggttgcta tttgttttga caaaaaaaca aatagtaata aaaaaagaga cgaaagaaac
     9961 attacctatt tttgtcaatg gagaccaata ccagctggaa aaagaactga tcttggaacg
    10021 gaggggaaaa taatgcgtat tctgattgcg ccagattctt tcaaagaaag tctgacagcc
    10081 ttagaggcag cacaagccat tcaatcaggt tttcaagaag tttttgttca ggctgaatat
    10141 gatctgctac ctataggcga tggaggagaa ggaacggttt catcattagc agacagttta
    10201 aggttgaagc ggcaaacagc agtcgttact aatgcatttg gcaaaaacaa agaaatccag
    10261 tacgcttcag acggtcaaac tgcgattttt gaaatggcag aaatcgtcgg attgaaagat
    10321 gtcccactaa accaacaaca gccacttagg ttatccacgc gaggagtagg agaactgata
    10381 cgacaattgt tgcaacaagg tgttcgacac atcatcatcg gcgttggcgg aagtagtacc
    10441 aacgatggcg gcatcggcat ggcagaaggt ctaggttatg ctttttacga tcaagataat
    10501 caaaaagtag aagcactggg agaaaatcta ggtaaaattg ctacagttga taaaacgaac
    10561 gtgcctattg agctgattaa agccaaaatc actgtcatta ctgatgttac taatctttta
    10621 tgcggaaaag aaggcgcttc ttttgttttc ggaccacaaa aaggcttgaa agaaaatgaa
    10681 ttagcagtgg ctgaccaagc catgcatgat ttttatatga gatttgcccc tcaagtaatc
    10741 gaggcatcgg gcagtagtgc tggtggtgga atgggtgcag gactgttagc atttgcttca
    10801 gggaaaatag tctctggcat cgactacgta ttggatttac tggagttcga taaacgggta
    10861 cagcaagcag acctcgtaat cgtaggcgaa ggacgtctag atggacaaag tctaaaagga
    10921 aaagcgccag ttgggattgc tagaagagta ccagagggcg ttcctgtgat agctatttgc
    10981 ggaagtgttg gtaaaggaag cgaaaaagca gcagatgttg gcatttcggc cgtttttccg
    11041 atccttcctg cagctgtgcc tttacaagaa gctttacagc aagccactga taatcttcat
    11101 cgcacagcta aaaacatcgc atctttactt caagtcgttc attttcaagg aggtaatcga
    11161 tgaaaatcaa agcagcacta ttcgatttag atggcgttct tgtagacaca gctcgttatc
    11221 attacgaagc gtggctggtt ttagctaacc agctgtccat tccattcact gagaaagaaa
    11281 acgagcaact gaaaggaata tccagaaccg agtcactaga gcgattgctg tcatttggaa
    11341 aaatggaaca gaaattctca gaaaaagaga aatcagcctt tgctgagcaa aaaaacaatc
    11401 tatatcttca agcgattcaa aaaatggatg aaacatccgt tttgcctggt gcaatagctg
    11461 ttcttgagta cttgaaaaaa acaaacatca agatcggatt gggatcagca agcaaaaatg
    11521 cacgcctcat tttagaaaaa acgaatttga caagttattt tgatgtcctt attgacggca
    11581 cgcaagtatc aaaagcaaaa cctgatccag aagtttttct caaaggagca cagcaattga
    11641 atgtaccgcc aaatgcatgc ttggtcattg aagattcaga agctggttgt caagcagctt
    11701 tggctggtaa catgcatgta ttgggaatag gtgaaaacat aaatcttcct tctgctgaat
    11761 atgtgattcc tgatttaacc gtttttgatc aagtacgttc gttttggcat ttatcagaag
    11821 ccgtatcacg tatataatag tttccgtttt ttctgtcaca agcaaaacgt tatcctcctt
    11881 ttggaaatca aggtatgatg agaaaaaagg gggattttat atgacaaaaa aagcagtttt
    11941 cgcaggcgga tgcttttggt gtatggtcaa accatttgaa gagcagccag ggatcatttc
    12001 tgtgacatcc ggttatactg gcgggcatgt accaaatcca acttatgaac aagtaacaac
    12061 aggcacaaca gggcatacag aagcagtaga gatcgaatat gatccagaag ttatttctta
    12121 tgatcagttg gtggaaatct attggcagca aaccgatccg actgacgcat taggacagtt
    12181 cgctgatcgc ggtgattctt atcgtccagt gatctactat aatgatgaag aagaacgaaa
    12241 tattgcagag cgttcaaaac aagcattgca agacagcggg cgattcgata agccaatcgt
    12301 gacaaaaatc gaaccaagaa gcgaatttta tcctgcagaa gattaccatc aggattatta
    12361 caagaaaaac aagctccatt acaatcttta tcgagaaggt tctggacgtg caggtttcct
    12421 tcgtcgacat tggggcgtca agaaataaga agccgaaaaa gctacttatg gaacaccgta
    12481 tatcagaggt tgtaaaacca gcattttgag ctaatactga gggtcagctg attgaacacc
    12541 atttagtgga gaggttgtga acctgtcgcc tagctccaag tcacaaggat ttggtaagag
    12601 aacactatat atcagaggtt gtgacaagtc gtttgtcaca accttttttc aatacctaag
    12661 atgaaggaca atcagcaacg cactgctcaa aaaaggaatg aaaggcagct tttccaactt
    12721 ttttttatga cagataaagt aaacagaaaa aacggatatt ccaaacagac tagcaagaaa
    12781 caagatctga agcaactctt ctagagacaa gccaccactc caagaaagca tcaacagagt
    12841 atcgccagct ccgagatagg attgaccata gcgaaggcac acaccgccta cgataaacgt
    12901 taacagcaca ctttgccatt ggaagacgcc ggcctgcaac caaaaaatcc ataaaattgc
    12961 ccaagaacag aatagcgtgt gagaatggac catgaagtaa aataaatcca tcaacgaaaa
    13021 tagcaaagcc gaaagcatcc atatgaacgt cgaaaaattt cttgcggaag aaaaagtaaa
    13081 gaaccaggca aaaagaccac cacataaggc ttctgccaac aaatagtaga agggcaaccg
    13141 acacctgcaa tagcggcagc gaaatcgttg gacgatgatc gagagaatcg gaatcaattc
    13201 ataccaagaa agcgaacgac agcatttgac acaatgagat ctaggataaa taatagagtg
    13261 acctaacgga atccgctggg cggttaaaca taaaaatgat ccgatacagc aaccaatgat
    13321 aaaataaagt aacacgccat ctcccccttt tcaaaaccaa gatacgcaag cttaaactaa
    13381 atttttgtac atacataaaa tttgaggtga aaaaatgaac gtttcaatcg gattagatat
    13441 cggaaccaca caaaccaaag cagttgcttt cacagatgaa gcccaagaag cagcatccgc
    13501 ttattttcgc taccctttga tccaagaaac acaagggatg gcagaacaag atcaggaatt
    13561 gatctttcaa ggtgtctgca ccgttataaa agaagtaatt aaacagttgc cttcagacac
    13621 aatcattcaa tttgtctcct tctcctctgc gatgcatggc ctgatattgt tggatgaaga
    13681 gaaaacaccg ttatctcgaa tgatcacttg ggcagataac cgagcagcgg acgaagcaga
    13741 aaaactgaaa tataccgtgc ttggcaaaga attttatcgg cagactggga cgccgattca
    13801 tccaatgaca ccattgaaca aaatcaagtg gctgcaaaac agccaacctg aattattcaa
    13861 aaaaactgct tattttgtcg gtatcaaaga ctatatcttt tatagattgt ttggcgaact
    13921 gatcagcgac tacggaacag cttcaggaac tggatatttt gatatccatg aattccaatg
    13981 ggcagaagaa gtgcttgact acttgaacat tcgaatagag caattgccgc aagtctttcc
    14041 ttccacctat caagtaacag gtctaccatc aaaaacagca gcagaactag gtttgccaaa
    14101 tgatatatca tttgttttgg gaggggcaga cgggccgctt tctaatctag ggttaggtgc
    14161 ttttggtgat acgatcgccg ctttgaccgt aggaacgagc ggagcacttc gttttattac
    14221 tactcagcct cagttccatc cagaaatgga gacgttttgt tatgttctgg atcagaaaca
    14281 ttgggtagtg ggaggagcga ctagcaatgg tgctggcatc tttgattggg cagggcatac
    14341 atttttacag gaagtcgttc aatctgctaa agaaaaagga gaaaatccat atgatgcact
    14401 gctttcggct atcgcttttg tgccagcggg tgccaatggt ctgatttttc atccctattt
    14461 acttggtgag cgagcaccgc tttgggacgc agatgcgttt ggcagtttta tcggtttaca
    14521 gcggcaacat acagaaaaag aaatgatgaa ggcgattcta gaagggatct gtctcaacct
    14581 ttatcggatt ttaaaaggag tttgccagga aaatcagctc acagagatcc gtgctacagg
    14641 cgggtttgct caatctccga tttttcgtca gatgatggct gatgtactgg gttacccgct
    14701 tgttttccct actgtcacag aagcatccgc attaggagct gtgattttag gctggagaag
    14761 catcggaaag atccaaagta tcagtgaagc acaggaattg atagaattga aagagcaagt
    14821 tggagtacaa gaaactgatc ataaaaaata tcagaaaatc tatccattat ttgtttcgac
    14881 acaaaagcat ttagcacaga cttatcagca atttgctcaa ctgcgagaag aattagctga
    14941 ttctgaaaaa tgattggctt tacggataaa aaagcgaact gaaaatcgtt ctcaggtaaa
    15001 tgattttgtt gtacttttca ttgaaagtct actattattt taagtgtaag aaaatgttag
    15061 gaggcaagaa agatgaaatt ccaacaaacc aaagaagtat taaatcaatt agttgcagat
    15121 ctaagccaaa tgtctgttgt tgttcaccaa acacattggt acatgagagg accaggtttc
    15181 cttacattgc acccaatgat ggatgagttc atggatgatt tgaacgctca gttagatgaa
    15241 gtatccgaac gcttgatcac attagacggt tcaccttatt ctacattgcg tgaatttgca
    15301 gataacacaa aaatccctga tgaaattggc cgctgggatc gtactattcc agaacgtttg
    15361 gaaacacttg ttgcaggcta tcgttatcta gcagatcttt accaaaaagg aatcgaagtt
    15421 tctggagaag aaggcgacga tccaacacaa gatatcttta tcgaattcaa aacagccaca
    15481 gaaaaacgta tctggatgat ccaagctcat ttgggcaaag cgccagagat cgacgcgtaa
    15541 aaactgttgt tgaacttgac cgaaaaagag gttgtgaaat cagcgttttg acctgagtat
    15601 tagagaaaca aacgtcaaaa tagttcctca tattttgccg ttttttgagc taataccgag
    15661 ggtcagctga ttgaacaccg tttaccaaga ggttgtaagc ctgccgttca gctccgagtc
    15721 acaagaaaca attttaaaaa acagcttctt atgttttatg gatattgtga cttgtgaccg
    15781 aggagttggc aggagaacac cgtttattca aaataagggg ttgtgacaag acttttgtca
    15841 cagcctcttt ttgtttttca ttttgacagg aaacgaccct tttatataaa agaaagaaaa
    15901 gaagtaaagt ttcatataaa tttttcaatc aagcagaaaa acgttgacat tgactagatt
    15961 agcgggtaat atgttaaatg gtattgtttg ccccacaaag agccccggaa actcgaagtg
    16021 tatgtcaaac atcagaactt aaattggagg aaaataacgt gcgtacaaca tatatggcca
    16081 aaccaggcga agtagaacgt aaatggtatg tagtagacgc aactgatgtt ccattgggac
    16141 gtctttcaac agttgttgca tctgtactac gtggtaaaaa taaaccaact ttcacacctc
    16201 atttggatac tggcgatttc gtcatcgtca tcaatgcaga caaagtgaaa ttaacaggta
    16261 aaaaagcgac tgacaaaatt tattaccgtc acagcaacta cccaggagga ttgaaatcaa
    16321 tcactgctgg cgaattgcgt gctaaaaatt ctcgtcgttt gatcgaaact tctgtaaaag
    16381 gtatgcttcc taaaaacact ttaggccgca aacaatttac aaaattgaac gtatacggtg
    16441 gagcagaaca tccacatgca gcacaacaac cagaagtttt agacatcaca aacttaatct
    16501 aataggaggg aatttcattg gcacaagttc aatatatcgg cacaggccgt cgtaaaaacg
    16561 cagttgctcg cgtacgtcta gtaccaggaa ctggcaaaat tactgtaaat aaaaaagatg
    16621 ttgaagaata cattccacat gctgacttgc gtgaagtaat caatcaacct ttcgctgtta
    16681 ctgaaacaaa aggcgcatat gacgtatttg ttaacgtaaa cggcggagga tacgctggac
    16741 aatcaggagc tatccgtcat ggtatcgctc gtgcattgct agaagttgat cctgacttcc
    16801 gtttagctct taaacgtgct ggcttactta ctcgtgacgc acgtatggtc gaacgtaaaa
    16861 aaccaggtct taagaaagca cgtaaagctt cacaattctc aaaacgttaa tatcctttat
    16921 atatcaacgt ttctggcact tttcaattat ttggaaagtg cttcttttgt ttttaagaac
    16981 acatttaaga acacatatca atcacactta ttcaatcctc tctgaaaagc ttcaaaaaca
    17041 gccttatcaa ctatatctgg tgtgttcaca tatatctttg ttgtagaaat atctgagtgt
    17101 gttaaagcat ttgaaatttg attcatatct gctccacctt gtctagctat agttgcataa
    17161 gtatgacgga gtttgtgtgg agataaatga actaagtcag gatgccttct tttaattgaa
    17221 ttgattcgat agtttaggta atcggcatga acaggacaat ttacttctcc agatggttta
    17281 gtataagtaa agagatactg atctaacgtt tgcttaattt ttaatttcaa caattgatca
    17341 gcttgtaccg atttccattc acttagcaat gtcattacaa cttctggtac ttgaatgacg
    17401 gtatcttttc gaccttttgt aaactttttt ctttgatatt tatctaaagt atgtttaaga
    17461 cggaccgttt gtttttcaaa atcaatatat ttccattgca aagcgtaa
//
LOCUS       DACDHJ010000039        17204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDHJ010000039 DACDHJ010000000
VERSION     DACDHJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.040"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            166..1932
                     /locus_tag="IV223_001025"
     CDS             166..1932
                     /locus_tag="IV223_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8711329.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2034..3293
                     /locus_tag="IV223_001026"
     CDS             2034..3293
                     /locus_tag="IV223_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8711330.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3440..4741
                     /locus_tag="IV223_001027"
     CDS             3440..4741
                     /locus_tag="IV223_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8711331.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4744..5595
                     /locus_tag="IV223_001028"
     CDS             4744..5595
                     /locus_tag="IV223_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8711332.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5608..6423
                     /locus_tag="IV223_001029"
     CDS             5608..6423
                     /locus_tag="IV223_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8711333.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6574..7740
                     /locus_tag="IV223_001030"
     CDS             6574..7740
                     /locus_tag="IV223_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8711334.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7970..8617
                     /locus_tag="IV223_001031"
     CDS             7970..8617
                     /locus_tag="IV223_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8711335.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8701..9645
                     /locus_tag="IV223_001032"
     CDS             8701..9645
                     /locus_tag="IV223_001032"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8711336.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9780..11231
                     /locus_tag="IV223_001033"
     CDS             9780..11231
                     /locus_tag="IV223_001033"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8711337.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11252..12088
                     /locus_tag="IV223_001034"
     CDS             11252..12088
                     /locus_tag="IV223_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8711338.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12088..12747
                     /locus_tag="IV223_001035"
     CDS             12088..12747
                     /locus_tag="IV223_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8711339.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12873..13562
                     /locus_tag="IV223_001036"
     CDS             12873..13562
                     /locus_tag="IV223_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711340.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13568..14524
                     /locus_tag="IV223_001037"
     CDS             13568..14524
                     /locus_tag="IV223_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8711341.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14568..15080
                     /locus_tag="IV223_001038"
     CDS             14568..15080
                     /locus_tag="IV223_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8711342.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15336..15884)
                     /locus_tag="IV223_001039"
     CDS             complement(15336..15884)
                     /locus_tag="IV223_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8711343.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15973..17154)
                     /locus_tag="IV223_001040"
     CDS             complement(15973..17154)
                     /locus_tag="IV223_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8711344.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5652 a         3069 c         3449 g         5034 t
ORIGIN      
        1 gtagactaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa
       61 ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgcttctcta
      121 tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca
      181 atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt
      241 cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctatgtgg agatccatat
      301 accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc
      361 aatgttcatg attattggga aattgaagtg acgagcgaaa caagtcgctt acagtatgcg
      421 ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct
      481 tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa
      541 atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc
      601 gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt
      661 aaagatccag accgagaaga ctttttcggg ggagatttgc aaggtgtgat ggatcatttg
      721 gattacttga cggaccttgg catcaatggc atctattttt gtcccatatt taaagccaca
      781 tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa
      841 aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat
      901 gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga
      961 gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag
     1021 atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca
     1081 ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca
     1141 acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc
     1201 gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat
     1261 atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca
     1321 gtgatgaatt atgcatttac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca
     1381 gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac
     1441 gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa
     1501 ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct
     1561 tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa
     1621 tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt
     1681 gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata
     1741 gtaaatgatc gtgaagataa gctctatttc acacggaaac tagagggaca gatcatctat
     1801 gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt
     1861 agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg
     1921 atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt
     1981 gcccattata ataagggtgt aacagcacgt tgaatacaaa ggaggaaatg aagatgaaaa
     2041 aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag
     2101 cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa
     2161 cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa
     2221 aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat
     2281 tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga
     2341 tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt
     2401 acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag
     2461 taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt
     2521 ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa
     2581 aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag
     2641 gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata
     2701 atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa
     2761 agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata
     2821 aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca
     2881 attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg
     2941 gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc
     3001 ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc
     3061 ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg
     3121 tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt
     3181 ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat
     3241 ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat
     3301 gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg
     3361 agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa
     3421 gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat
     3481 cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg
     3541 tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca
     3601 tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat
     3661 tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt
     3721 gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg
     3781 gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact
     3841 tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac
     3901 tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg
     3961 ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt
     4021 cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat
     4081 tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat
     4141 tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt
     4201 caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt
     4261 tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat
     4321 cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca
     4381 atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg
     4441 cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca
     4501 gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg gcggtcccgg
     4561 aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac
     4621 gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat
     4681 cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta
     4741 acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact
     4801 tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt
     4861 actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact
     4921 ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca
     4981 ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat
     5041 acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt
     5101 cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa
     5161 gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat
     5221 acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa
     5281 cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg
     5341 atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct
     5401 aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt
     5461 tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta
     5521 ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc
     5581 ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca
     5641 ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc
     5701 ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat
     5761 cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa
     5821 ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa
     5881 acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc
     5941 gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa
     6001 tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt
     6061 acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca
     6121 atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt
     6181 ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat
     6241 acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca
     6301 gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata
     6361 actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa
     6421 tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt
     6481 tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa
     6541 cgtaataggg tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa
     6601 ttattttcac gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa
     6661 caatacggag atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca
     6721 atgaaagaga ttacaagtaa cgcatctttg gcactaggag aagcctatat ggatcatcga
     6781 atcgagatcg agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc
     6841 tttttacgag gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa
     6901 aaaaacaaag aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg
     6961 ctagatccaa ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa
     7021 caagcacaaa tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa
     7081 acgttgcttg atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat
     7141 aatgtcaagg caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga
     7201 ataaaagaag aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta
     7261 aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa
     7321 aatcttgaag gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg
     7381 atccatggaa tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt
     7441 ttccctggcg gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat
     7501 ttgcaactga ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg
     7561 acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat
     7621 cgaatgtggg atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat
     7681 gtgattcaat atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa
     7741 ttgaggcaaa agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca
     7801 gaagtgaact tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt
     7861 atattttcat aataaaagtt tggttcttta atttttaaac atgacagaaa attttttctt
     7921 cgttatactt gtaatagaat actattttat aacaaagggg atcgaatcaa tgaaaaaaac
     7981 tgttatttat actagtttat ttgcagcttt gtttttagct gcttgtcaat caaataccaa
     8041 tcaaggcaca tctgggaata cgtcttccag tagcgaacag cgctcttcac aggtatcgga
     8101 ctcctcaaca aaagcaagca catcttcgtc tagccagaca aaaagttcta gtgaagcaag
     8161 tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc aagataaata
     8221 cccagatact gcaattacta gtttggaatt agatagtgat tggggatctt acttctacaa
     8281 gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa
     8341 aatggagaca gagaatccag aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa
     8401 agaagatggt ctggatgttg ataaattgat cacgattgag gaagcagcaa aaatcgccgt
     8461 cgacaaagta ggaagcggta cggcaacaga ttgggatttg gataaagatt taggtactac
     8521 ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca
     8581 aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga aaacaagagt
     8641 tgatttttct tttagtcatg atacactaga caaaataact tgtttgaagg agcgatttac
     8701 ttgagtttag aaacgacctt tgccaatcat atctttgcca atcctttgat gaatgcatct
     8761 ggtgtccact gcatgactac ccaggaatta gatgaactgg ctcattcaga agcaggagca
     8821 tttatcacta agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt
     8881 gatgttcctt tagggagtat caattctatg ggacttccaa acttaggttt ttcttattat
     8941 ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt
     9001 tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt
     9061 gggtttaatg ggatcactga attaaattta tcttgtccca atgtgcctgg aaaaccacaa
     9121 ctagcttacg attttgaggc cacttatgaa acactgaaag aagtattcag catcttttct
     9181 aagccgctag gaatcaaatt gcctccttat tttgattttg ctcattttga tcagatggct
     9241 gatatcttga atcagtttcc attgacctat gtcaatgcta tcaacagtgt gggcaatggt
     9301 ttgtatatcg atacagaaca agaagcagta gtcatcaaac caaaagaagg gttcggcggc
     9361 attggcggag agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt
     9421 ttgaaaccag aaatccaaat cattggtaca ggtgggattc ggacaggaca agatgcattt
     9481 gaacatctgc tatgtggcgc aagcatgtta cagattggta cagagcttca taaagaaggg
     9541 ccggaaattt tttctagaat cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat
     9601 acgtcaattg acgaatttaa aggcaaatta cgcaccattt catagcaaag agacgaaggt
     9661 catgattgat cttcgtcttt ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg
     9721 tactcattgt atgttagacg aacttgtggg aaaataacag aggcaaaaag gaggataata
     9781 tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac
     9841 tagttacgga tatacagaaa aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg
     9901 cgcttgaacc attcgagagt aattcccacg agctattgat tccccagtca gaaaaagggc
     9961 cattggctgg tatcccattt cctttaaaaa tgctcggtca agaaaaaaag ggatggctag
    10021 ctacttcagg atctcgttta tttgaaacac accgagcttc tcatacatct aattatgtgc
    10081 ggcaagcaga agcgataggt ctagttcctt ttggacaaac caatgcgcca gagtttggtt
    10141 tcaaaaatat tacagaccca gtcatctatg gcccagcaag aaacccttgg aaccttgaat
    10201 gtacaccggg cggatcaagt ggaggagctg ctgctgcagt ttcatcagga atcgttcctt
    10261 tagctggtgc tagtgatggc ggtggttcta ttagaatacc cgcttcgttt tgcggattga
    10321 ttgggctgaa accttcgcga gggacgatgc cagttggtcc atatgcttgg agaggatggc
    10381 aaggagctgc tattgatttc ggcttgacag tttctatgag agacacggag gctttatttt
    10441 atgggatgag aagtatccat tccggtgcgc catatcaagt atcgccagtt gagtggcaaa
    10501 cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg atccactcac
    10561 agattagtga agaggcagta cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag
    10621 ggcatgattt gacagagatt ccttacccac tagatggtcg taaactgatc acgtcttatt
    10681 atcagatgaa tggcgcagaa acagcagcaa tgatccgctc gattgaaaca ggcatccagc
    10741 gagctgtcag aaaagaagaa cttgaaccca tttcttggac tttgttccaa tatggtcaga
    10801 agctctctgc cgctacttac atcgattctc tgcatgtatg ggatgaagca gcaattgtca
    10861 tggaaaacct acatcaagaa tacgatctat tcctttcacc aacgactgca tttaccgccc
    10921 ctaaaatcag cgaagatttg caaagtgatg cgataagagc acagatggaa cacatcagtg
    10981 attgttctga agtagaaggt ttagacgttg tttcttccat gtttgaggag agcttgaaga
    11041 tcacacctta tacacagttg gctaatctga ctggacaacc agcaatcagc ttgcctactc
    11101 atttgacaaa agaaaacatg cctctaggaa tccaatttat ggctgccaga ggaagagaag
    11161 atttgctatt tcaaatcggc tatctatttg aagcaaatca tcagttccat ttaccaaatc
    11221 catggaaata gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt ttgctctaat
    11281 aactagtgcc ttttttattt taagcgcttg cggtacagct gccgctaatc aggaagataa
    11341 cagctggagt aatgtagaaa aaaagaaaga attagtagta gcgacttctg gcacactgtt
    11401 tcccgcatct tattataatg atcaaaatga attagttggt tatgatgtag atttggcaaa
    11461 agaggtggct aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg tggatggaca
    11521 aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg gtatcacaaa
    11581 agaaagagaa aaaaaattct ccctttcaga gactttcaaa tattcatttg acagtatgat
    11641 cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc
    11701 agctggcgaa cccaatacta gctatatgcg tttagcggaa aaacacggag ctaaattagt
    11761 cacatatgac aatgccacga atgatcagta tttaagcgat gtagcaaatg gtcgaacaga
    11821 tgttattcta aatgattatt atctgcagaa aatggccgta tctgctttac cagatatccc
    11881 tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa agtggttttt tattgaaaga
    11941 aggaaatgat gctctgaaaa agaaaatcga tggcgtatta aaagagatga aagaagatgg
    12001 gacgacaaag aaaatatctg aaaaatattt tcaaactgat gtttcagtca aaccgaaaga
    12061 aacgatggct gagtcggtga gtgagtgatg tactttcctc taattgattt ttccctcatg
    12121 tggacttcac ttcctttcgt actgcaaggc ttggtataca cattagggat tggcttcgtg
    12181 tcgtttcttt taggaaattt ggtagggttg cttttgacag ttttaggtct tttggattgg
    12241 ctgcctctga atgtatttat tcgtttttat ttatcgttct ttagaggcat tcccgcacta
    12301 gtcctgctgt ttctgttata tttcgggtta ccttatcaat taagtgcctt aacggcttca
    12361 gtcatctgct ttacgattac cagcagtgca tttattggtg aaatctatcg aggatcacta
    12421 gcaggcgttt cttcaggtca gtgggacgct ggtttagctt ctggtttttc tttctttggg
    12481 gtattgaaat atatcgtttt gccgcaagct tttcgaatct cagtaccggc actcagtaac
    12541 gtagctatgg atctagtgaa aggtacttcg ttagcagcaa caatcacagt gccagaaata
    12601 tttcaaaaag caaagatcat aggcggtcgg acattcgatt atttttctct ctatatttta
    12661 gttgcactga tttattgggt catttgtatc gggatcgaaa aagtgcaaca gtatttagaa
    12721 aaacgttcta tccatcagtc aatctgaaaa atagaaatgg tgctagcgtg gctggcacca
    12781 tttctatttt ttgtttcttg tatattctgc ctagtatttt aaaaccattt catgtaaaat
    12841 gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac cccgctaacc atcgaaatag
    12901 aatcctcgtt ataccaatat tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga
    12961 tgcctgatga ccaaggaatt gtagatacgc ttagctgctt gacaaaattt gatggaagcc
    13021 gagaatggcg atgctatgag ctgaaagtga caaaagcaga ttttcgctct acagccaaac
    13081 tgtcatttgt tggacattat aattattttg ttttaccaag taaactatat gaagaagtaa
    13141 aaactgaaat ccctacagga ataggtgttt tagtgtatcg tccgtacacg gttgaagagg
    13201 agatgcctgc ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg
    13261 taccggaaga agctttgaca aaccgcttca tggcttcttt attcagggaa gtaagaaaag
    13321 caaagcaaat ggagcgaggc gtaaaatatt ttccaaaaga tcaattgtac aaagaattga
    13381 aaagacgttc agaaaatcaa gatccatttt ctaacgaacg atattatgaa cggtttttag
    13441 aggaaatagc tagtcaggca atcaatgagt tgcaagaaga agttatcgcg cttcagcatg
    13501 attatgaata tttgaggcaa caacagcaga tccgtcggtt gcctacagag ccattggagt
    13561 gaaaaatatg tactatcctt atttaagagg aaaacaattt gacctattag cattaaaaga
    13621 agcactgtcg cgaggattgc tctccaataa aatccaacca gtgatcgaac cagtacgtga
    13681 ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt
    13741 gatccaaaat ccccaagtag gacaattcaa attattcgat cagcatgtgc acagttggac
    13801 agtcaaagaa aatagctctg tcgtccctgc acagatctta acgccagaaa atctcagtga
    13861 agtactggat tcacctccag cgtttcttgt tttcgatgga cagcactacc caaaagatac
    13921 agaggtgtgg aaacagttgg caggagttga ttcgaaattt ttgataccag atactagtcg
    13981 ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa
    14041 gcatgttgaa agttatgcag ataaaacaga tgactttttt agtgatgact atcttttttt
    14101 ccacgcagac ggatatattg gattttctga tttcacgatc gaaggtagcc gttatttcga
    14161 taaagggttc cctagccgag cgcttgctat ccatctaacg tatattgatg cttacggcaa
    14221 tatacgcgtg aaacattttg tctctgacag taacgactct gctaaagacc aagcattgaa
    14281 gtttcttgaa gcaggagata aaatgaaaaa atggatcatg cgtcaccatc atcaactctt
    14341 gattacttca ggaatgattg aattattggc tctttaccag caacaaaaat tccctggact
    14401 gggagtgttg aaaaagtggt cacttatgca tcatttggaa cttattagtc acctgctaga
    14461 tcatccaaaa gattggctta aatattattc aaaagttcag gaactatatg aagtgatcca
    14521 atgattagat aatgtaaatg aaaaaggaat agaaaagagg aaactggatg tatattcggc
    14581 aaacaaggaa agaagaagta gaagaagtgg tagaaatcat taatgccgca gcggatttca
    14641 ttgcttcgca aggctctcct caatggcaaa acgggcagaa accaacaaaa gaaacgattt
    14701 tgaacgatat tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa attgctggaa
    14761 cagctgcatt agtctcaagc gtggatcccg tttacacaaa aatagatggt agttggaaag
    14821 aatcaactgg tccttattta tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa
    14881 aaaaactagc aaaaccgttc ttagaacatt tgttgacgat tgcagccagc aaaaatatcc
    14941 aagatatccg tatcgatact tatccagcta atgaaccgat ggaaaagacc atctattctg
    15001 ctggattcac ttatcaaggg atgattgaat ttccttttga acacggcgaa agaaaagcat
    15061 atcaaataat catcaattga ctataaaatc atctgagcaa agataaagac attaacacta
    15121 ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa
    15181 acaagaaaga atttccgaaa atgcttctca aatttttgtg gaattttagc gtattttacg
    15241 aaatagctgc tttctgaaac tcccttcatc gagaggtcgt gacatagctt ttgtcacgac
    15301 ctctcggctt atcctacgag tattattcga tacatttata tcaataatcc tttttttatt
    15361 ttatctgcag cttcttggaa cgctccatcg tttgacacga ctaaaagtga ggggatagct
    15421 ttcaaacgtt tcaactgtgc taaatcttct acataaaccg ccagtctggc tgaaatctgt
    15481 tcagatgaat ctccacgttt tttcaggcgc gattgcaatg tttctttaga gatcgtaata
    15541 aagataggac ggactaccgt cccgaaatac tcatataaat gccaaaaacc atttgctgtc
    15601 aaaacggcgt aacaatttcc tactgtcagt ttctttttaa tttctgcttt actggaacca
    15661 tatttatgtt gagcatattg gtcccactct gcaaactcat ttcccttgat catctgattg
    15721 aatgtattgt cagaaacaaa ataataatcc tcttgatctt tttccccagg tcttggtgat
    15781 cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt
    15841 tttccagaac cagaaggacc gataaataca tagcaataat tcattgtcat ttctcctttg
    15901 atgattgaac aagtgaaaaa gctttggaaa atatttccaa agcttttaaa agtaactttg
    15961 cttttttgta tattatacca gcaagccagt cacaatagca cttaaaaagc tgactaaagt
    16021 cgctgttagc aaaattttca atccgtgttt tgcgacaaga tttccttttt cttcatttaa
    16081 tcccttcatc gcaccagaaa tgatcccgat agaagagaag ttggcaaatg aaacgaggaa
    16141 gacagatata attgctgttg tacgctcagt aaaatggaaa ttaccatttg ctaattctgt
    16201 catagcaaca aattcatttg taaccagttt agtcgccatg atactaccgg catccactgc
    16261 ttctttccat gggattccgc tgatgaaggc aagaggggca aagatgtaac ctaggatctc
    16321 ttggaaagtg atgccgaata tcccgtggaa gatagcattg atcatggcaa tcaaagcaac
    16381 aaaaccgata agcatagcag caaccgtgat cgccacatgg aaaccatcaa gaatgtattc
    16441 tcccaacatt tggaaaaatg tctgttcttt gttttcttca atgatcagct catcttcttt
    16501 ttcttctaat acatagggat ttacaattga agccagaata taaccgccaa ataagttcaa
    16561 aacaagggca gtgatcacat atttagaatc gatcagtgcc atataagatc cgacgatcga
    16621 catagaaact gtagacatag ccgatacact catggtgaac aaacgtttct cagataggaa
    16681 gggtaattcc ttttttatgg aaataaaaac ttccgattgt cctaaaatag cagaagctac
    16741 tccattataa gattccaatt tgcccatccc gttgatttta cttaatccta gaccaacagc
    16801 tttcataaat aatggaagaa tcttgatata gcgtagaata ccgataatag cagaaataaa
    16861 tacaattggc atcaatacgg aaagaaagaa aggtgcactt cctttatttg ctacaccggc
    16921 aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa
    16981 tacagaaaca accgctgtac caaaagttgt acgtaataat acgaaaccaa gaacaagctg
    17041 taaaacgaac atcacaatta gtggtttcaa tttgattgct tttttgttac tgctgatcaa
    17101 aaaagcaagc cctaatgtga caaataaacc aataatacct aataaataac tcaaataatc
    17161 actcctattc caaattccgt tttattttac ctgttttgca aata
//
LOCUS       DACDHJ010000040        17166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDHJ010000040 DACDHJ010000000
VERSION     DACDHJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.041"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..444
                     /locus_tag="IV223_001041"
     CDS             7..444
                     /locus_tag="IV223_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711345.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            539..1423
                     /locus_tag="IV223_001042"
     CDS             539..1423
                     /locus_tag="IV223_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8711346.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1647..4814
                     /locus_tag="IV223_001043"
     CDS             1647..4814
                     /locus_tag="IV223_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711347.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATMGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4834..5379
                     /locus_tag="IV223_001044"
     CDS             4834..5379
                     /locus_tag="IV223_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP8711348.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5407..5862
                     /locus_tag="IV223_001045"
     CDS             5407..5862
                     /locus_tag="IV223_001045"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP8711349.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5859..6617
                     /locus_tag="IV223_001046"
     CDS             5859..6617
                     /locus_tag="IV223_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8711350.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6703..7443)
                     /locus_tag="IV223_001047"
     CDS             complement(6703..7443)
                     /locus_tag="IV223_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8711351.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7504..8757
                     /locus_tag="IV223_001048"
     CDS             7504..8757
                     /locus_tag="IV223_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8711352.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9079..9306
                     /locus_tag="IV223_001049"
     CDS             9079..9306
                     /locus_tag="IV223_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8711353.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9429..9755
                     /locus_tag="IV223_001050"
     CDS             9429..9755
                     /locus_tag="IV223_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8711354.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9823..10536
                     /locus_tag="IV223_001051"
     CDS             9823..10536
                     /locus_tag="IV223_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8711355.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10846..11697
                     /locus_tag="IV223_001052"
     CDS             10846..11697
                     /locus_tag="IV223_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8711356.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11818..13683
                     /locus_tag="IV223_001053"
     CDS             11818..13683
                     /locus_tag="IV223_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8711357.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGIFSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13731..15140
                     /locus_tag="IV223_001054"
     CDS             13731..15140
                     /locus_tag="IV223_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8711358.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15248..15949
                     /locus_tag="IV223_001055"
     CDS             15248..15949
                     /locus_tag="IV223_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8711359.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16108..16587
                     /locus_tag="IV223_001056"
                     /pseudo
     CDS             16108..16587
                     /locus_tag="IV223_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5723 a         2887 c         3702 g         4854 t
ORIGIN      
        1 agataaatgg agtggttttg caaatttctt ttatttctag aagttattca cgtatacgga
       61 tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta tataaaatat
      121 attcatcaat tttccgtttt ggtgttaggc acactaatta tagcggggat tgtcgcattt
      181 attattcaag gccgtgacat attaacagta catcaagagt ttttcagtta tgcatttggt
      241 attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta ttacattctc
      301 ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg tatacaatgc
      361 ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt catagtatgg
      421 atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca tcacagccta
      481 atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa agaaagtgat
      541 gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa aggaatttga
      601 gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa
      661 aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt atcatagtgt
      721 gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga
      781 acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg ctcgagcatt
      841 agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat cagtcaagcc
      901 attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa ttggctctat
      961 ccctgaatca atcaatgatg tagatgcgga gaagaagacg aaatttggaa aactagctta
     1021 tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc acgtagaagt
     1081 tgatggaaag aaggaagaaa ttgagagcag taccttgctg ataggactaa ctaactcagt
     1141 cggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc tccatttggt
     1201 ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc tattaaaagg
     1261 agtagatgaa tctacgaaaa atctagccta ccttacttgt gagcaattag ttgtgtcatt
     1321 agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata aattacctgt
     1381 gacaatcaaa gtattgccct cacatttaca ggtttattgt taaaatggag gttgtgaccg
     1441 aggagttggc gggagaacac cgcttgttcg ggataaggga ctgtgacaag acttttgtca
     1501 cagccttttt tttgtacaaa aaagaaattt acacctaata gaaaaatttt tccataaaaa
     1561 tacatatttt aaatatgaaa gtacaattgg aaatagatta aaacgataaa ataaaattgt
     1621 caaaacgtca gagaggatgg tggagaatga atcaatgtgt aaaaaagctg attgcgtcga
     1681 ttttgtttgt cttccctctc ttgatggtga catccgtttt tgccgatgta gagaaaacaa
     1741 acgaatcaac gcaatcagag aatcaaagtt ttgacaagac tactttttca gaagaatcga
     1801 ttacttctga aactacagaa tctcaaagtg aggcttctac taataaaagg caagaagaaa
     1861 ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt gaagcggcac
     1921 tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa gaactggcgc
     1981 agcttctcgc agacgaatca gtaactaaga tccgactgat tcagcctttg actttagaca
     2041 gggaattaga gatcaaacga gatatcgtta ttgattttgg tggattcgct cacaatttcg
     2101 gcacacacca tatttatatc aatgagatac ctcagtcgat cgagttccag aattttaaag
     2161 gaacagctgc acatccaggt ggattgacac caaatatcga tggaaatgcc attatcattg
     2221 cttatatgac cgatttttgg ggaagcagat atcattttac gggagaaata aaatttaccg
     2281 ggacacttga tttgtatgat gggtccaaat taggactgat ttacgctcct cgtgcaactg
     2341 tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa ggactgaatg
     2401 ccgctccggg taaagcctat ttttgtagaa gctatcaatt gaatgtgatc aatggtagtc
     2461 aactttatgg accgtatcta gggaaattct acggttttct agattcagga gatgagactg
     2521 gtagtggaaa aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc tctcttgatt
     2581 atgatcgggc agatggcgtt agcgatgaag gagaggcagt cgatacgcta cctggtaatg
     2641 tcatgtttga agtcagtggt tcgggaagcg aattttcggt aaatacaaag atcaatatta
     2701 ccaatgacaa caatcgcgga atcatccaaa tgagaggttc aggatcgaaa gtcactgtat
     2761 cgaatggtgg gaaaatcacc atcaatacag caactatggg cggatttcgc ttgcaaggag
     2821 attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg
     2881 acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct ttgatcgtag
     2941 aaggagccgg atcagttatc gacatcgata agcagagtgg gcgttccagc gcagttcgtt
     3001 ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg gtaagaaata
     3061 caggtgatgg caaatcttac gtgaacagac tcaatcaagg aaatcaagcg atccaatttt
     3121 atgacgcttc tggttttcta gaaagtccag gttcagctga ttttacagtc actggagaaa
     3181 aatcaaatat cgatatacgc gcagattccg gagcaactgt agatacaact ggagatattg
     3241 atttaaattt tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa acaggggaag
     3301 ataagaccgg catcatcagt ggggatgtgt tgaatgtgga cttagaatct cctacctatt
     3361 ttgatttcca aaacaaacgg attggaagcg agaccaatgg cataggcggc tgggtattcc
     3421 aaggaaatgg agattcttct ttaaagatcg cccattcaga aatcgcttta tggcgaaaaa
     3481 caggcgcaac attcgataac tttgaaaaag acccggatta tttttctgat atgacagact
     3541 tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca ggtttaacaa
     3601 atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataatc
     3661 agctcccgat gattgaatcc ttaagagtgc caacgaatgc agataaaaaa atctatgggc
     3721 atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag
     3781 aagtgacttt acgtattact tacgcagata cgacgaaatc atctgtagaa attactgctc
     3841 tcacgcaagg acaaacagat gaaaatgaag gggcaggata taacccatgg ggagaaggcg
     3901 aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga gagattaaaa acggaggatc
     3961 aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca gaactggact
     4021 ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc taatttaagt accacattga
     4081 tcaatgcagc ttctcgcacg atcacaggta caggtgaagc aggagccttg gtttcattga
     4141 aaaaaaatca agctgttttg tcagaaacaa ctattgatga tacaggggaa tttgttcttt
     4201 ctatcccaga aaatcaactt tttgcaggag atgtattaga aattgtatta aacgaccaag
     4261 caggagaagc tactgctaag ggtgtgcaga acaagccaag taccaacgat gaaattggga
     4321 atcacaatcc aagtgatacg ccagttgttt atcatgatgc cccgcctttt gcacctgcta
     4381 cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc ttccaaactc gattttggtc
     4441 agatcaaagc aactggtttg acacaagaag catttggcca aatggatata caggaaaaat
     4501 tatggattta tgatcagcga gaaacgaaaa atccgtggat cttaacgata aaattatctc
     4561 agcctttcac aaatgatgca ggattttcta tgcaggatgt cctatatttc aagaaaggag
     4621 aggagtatca attagtgaat gaagaagaac tggaaattgt cagaggacaa aattcaacga
     4681 atgtgcaagc agattattca gaatatttaa ataacggtcg ggcgtttaaa cttgtcttaa
     4741 ccaaagaaaa tcagatagct ggtaattact cagcagagat aacatggaca ttagcagatg
     4801 cgccgacaga ataaagatca aggaggatga ttgatgaatc gaatatggaa taaaagtcat
     4861 cgaaacaact gctctttact tatgatcctt ctccttggat tcatcctttg gcagacacct
     4921 gtccatgcct ccgaattcaa ttttgcggta gattttgaat taccggctaa tcagattgat
     4981 aagaacaaaa catatataaa tgtaaggatg gagcctggga aaacacagga aatcccctac
     5041 acggtcttta accgaacaga taaatcagta acagttgaga cattgatcca ctccgctacg
     5101 acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga tgaaacatta
     5161 agttatgata tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc tcctcaaagt
     5221 gcggtcactg agacactttc actgaaagcg ccagaaaaag agctggctgg tgtggtcgca
     5281 ggagggatca cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac agttggtgtc
     5341 gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa agagtcttcc
     5401 ccagatttga aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa tgtgattaag
     5461 gcgaatcttc aaaatccaaa tgcttcttat atgttcggct tgactacatc ggttgaaatc
     5521 tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat agatgtagca
     5581 cccaatacaa attttgattt acttgtccca ttagaaggaa aaaaattagc tccgggacat
     5641 tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca taccgatttt
     5701 gaaataactg atgatgcagc ggaccaattg aatgaaaagg atattgattt aaaaaatcag
     5761 ccagaaacat ttgactggcg atggctgata cttgttggag tcattcttat tggcttattt
     5821 gctggattat ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca attgcattaa
     5881 gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct gagaataaga
     5941 gctcagccac tacagaagga acgatttctg tgaccaaacc agatgctgca aatcctgaag
     6001 acgttgttcc agttcctcct gttgatccaa atgatcctga tactcctttt acacctgacg
     6061 aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat cttgattttg
     6121 gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaagc ggaacggaca
     6181 gtactggagc acaaacacaa gtacagaatt tcgtacaagt aacggataaa agcggaaatt
     6241 atgccggttg gaaattatca gtcaaacgaa cagaatttat tggtacaaca tctgatacga
     6301 ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat agcgcaacta
     6361 atagcagtgg aatgccatct accgtttctg gaactggcgc tgaaggaatc gaaatcccag
     6421 cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc acatggattt
     6481 attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt gttcctgtat
     6541 ccaattatgc tgtagacaac tacgtttcaa cattcacatg gtcgttaaca gatgctccta
     6601 cagaagagga agcgtaaaat catatcaacg atttcaggga gtggagccga ttcaaggccc
     6661 actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg aagcagtttt
     6721 ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa cgagtaagga
     6781 gtcaccgttg aaaaattcta acacataatg agcagaatca attttctgca attgcgataa
     6841 gttgatacag taagatttta aaagtaaaaa gtaagcatac ttttctaact ctgtcttcaa
     6901 ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca taacttggtt
     6961 tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga cgagacttcc
     7021 acctttttga tcaataaaga ttttatcgac atttacgatc tgctgttctt tccaattgat
     7081 ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga tatatccaat
     7141 cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg tgaaaaaaac
     7201 aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac ctgtgagtga
     7261 ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtgc ccaactggat
     7321 caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat gatgattgac
     7381 caattctctg atgatccatt cgctgtgaaa ctggttatct tctatgataa aatacttttt
     7441 catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca atggaggaat
     7501 aagatggtcg caacagtgac actttatttg acctttactt tgattttaga tttaggtatc
     7561 tatttattct tttcgaaaca actgctagta aaaaacagct ttggttgtat agctttgatg
     7621 gtggtgacac ttttacttcc tatccctaaa gtgagctttc cttacctagt tggcttatta
     7681 ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt gatcagtaat
     7741 cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat ggattattta
     7801 ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt tattttctat
     7861 ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt tacttatcca
     7921 actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat ttacttggta
     7981 ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt gtcgatagtc
     8041 atcagttttt tttctttgtt tttttccatt atgttgatca gtttccaagt aaaagaaaga
     8101 caacatcagg aatcgcttca actgtatcaa ataaatgaag aatattacca tcatctagag
     8161 gaattcaaac atgattataa aagtctactg ttttcattaa aaattactct tcaggaaaat
     8221 aaaaaagaaa cgttggaatt tttagaaata ttagaagatc attctgcagc tatttttgaa
     8281 aaaccgcaaa tacaacagct aacaaatatt gtatccccag ctgtcagggg tgtcttttta
     8341 aaatttctag aaaaagcaga atcggagaaa atcccgatcc aagtagttat tcctgtcgaa
     8401 gtgaaaacaa tcccgattga tctggttgtt tttattcgct gtttatctat ttttatttct
     8461 aacgcattcg atcatcgtgt agccgatcag agtccgatta cgatcaatct gctggaagaa
     8521 aaagagggat cacgttttag tatagcgaac aaggcaaatc caacgaattt gacgctatcg
     8581 gagatggtca aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct atacagtgcg
     8641 aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa tcgaattttc aaaacagaat
     8701 cactattttt ttgtagaaat gtatctggca tctagaaaga gcaaatcagc ctactgactt
     8761 actgtcaaag gataagtttg aggaaagtct acacggagat agagacaaca acacagcata
     8821 ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc tgtttctttt gctatgtagt
     8881 taaacaaagt agttgacact tccgctactt agtattagta cgaaagtata cttcatgata
     8941 ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa ctgctttgct
     9001 tacaccagat aaagggaaga gcaatatcgc ttattttagg cgtggagaag caagcaaatc
     9061 cctacgatca caaaaaaaat gctgacccag cagttaagag agttagaaca agatgaaagt
     9121 atccatcgaa aagcctataa ccaagtcccg cctaaagtag agtactcgct caccgaagaa
     9181 ggaaaatcat taagagagat tctagtagcg ttgtctgttt gggaagaaga acgtatcgaa
     9241 aaacaaaaag cctcaaggga agaagacacg ctactgcata cacatggtgg ctacctaaat
     9301 tattgatttt ctatgacatt tattttaaat aaaagaaaac aggagttttt ttaaaagcat
     9361 catctatttt ttatgtaaaa aaatggtatg attaatcaag taagaagtga ttggaagaag
     9421 gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt aattggcgac
     9481 aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct gaattgttag
     9541 aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa cgaatcaaag
     9601 aagaactggc agatgtattg atctattcat acatgatggc agacaacctt cacctggatc
     9661 tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct gtagaaaaaa
     9721 gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg ctgttggaaa
     9781 gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa aaaaggaaaa
     9841 gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg actagctttg
     9901 attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa atatgctata
     9961 gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag ttttgacttt
    10021 gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag aggaacagat
    10081 ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc ggttttcaaa
    10141 ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga tcaagaaatg
    10201 ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt gctgtttaca
    10261 ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga aaatgtgtat
    10321 acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt actgaatgaa
    10381 gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc cgtcacgcga
    10441 ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac agatgatatg
    10501 agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata agtcgttaca
    10561 gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct gaaaaaaaga
    10621 gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg tgttccagtt
    10681 tttttatttt ttatatgaaa aggcttgcat aaataaaaat tcgatggtat gatgattttg
    10741 aataagaaat ggattgttac tgtacggcag gcaaaaccag aattcagcta agaaacagta
    10801 ggcgaattct aggttttttt atttttcttg agagggcgaa gcaaaatgaa aattgataaa
    10861 attttgaata acaatgtcgt catttcgaaa aatggattcg gggaagaagt cgtttgtatg
    10921 ggaaaaggac tagcttttca aaaaaagatt ggggacgaga tttcgccaga agcggtccaa
    10981 aaagaatttg ttttaagaga ttcttttgct aaaaaccaat tccagcagct gatggctgat
    11041 attccggcag aggagatcga atgggtcaaa caagtagtcg aactggctga agataaacta
    11101 aaagttgaat tttcgcctaa tatttatctg acattgaccg atcatcttca ttatgcgatc
    11161 acacgagcaa aagaaaatat ccaactgcct aatccattat tatttgaaac aaaaaaattt
    11221 tatccgaaag aatatcaagc agcgaaagag gctcttttgt tcgtgaaaca aaagacgggt
    11281 gtggaacttt ctgaggatga agccggtttt attgcctttc attttgtgaa tagccaacaa
    11341 ggaaacgaag acatgcaagt gacgatgacc gctaccacaa tggtaagaga tatactgagt
    11401 atcatcagta aattcttcgg catcgtgttc gatgaagaat ctttgaatta tcagcgtatc
    11461 attactcatc tgcagttttt tgcccagcgt tatctcaaag gggaaggatc tgacgagcaa
    11521 gatgaatttc tctacgcatt agttcaagga aagtatccaa aagcgtttca ttgtgtcgaa
    11581 cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa tggggatcgc agagcagatt
    11641 tatttaacga ttcatattca aagagtagta agtgagaaaa aaagcatcca atcataagga
    11701 ttgttactgt atggcaggca aaacctggat tgaaaaaata ggttcacgaa tgtttgtgac
    11761 tgtttttttc aatctgggtt tttatttttt aaataaaaaa gaaagaagga aaaaacaatg
    11821 gataatcaag cagttggtcg tcgtgtatgg gaagcggtag gaggacaaaa gaatgtcaaa
    11881 agtttagtac attgtgctac gcggttgcga ttcagattaa aagatgaatc tttagcagat
    11941 acgcaaaagt taaaagaaga cccggacgtg atccaagtcg tacaaagtgg cggtcagtat
    12001 caagtagtga ttggcagtaa tgtagcagat gtttatcaat cgatcgtgga tgaggaaggt
    12061 cttgcagttt cggaggatgg aaaagaagag tccaaaaata ttttgaatcg tctgatcgat
    12121 attatctcta gtatatttac accttttcta ggggcgatgg cagccgccgg ggtattgaaa
    12181 ggattcttat cattggcaac ggtgatgggc tggctgacag ctgatagcgg tacttatcaa
    12241 atattgtttg ctgcagcaga tggtgtcttt accttcttac ctgtcatgct tgcttttaca
    12301 gcagctaaaa agttcaaagc caaccagttt ttagcagtag caattgctat ggcacttgtg
    12361 tatccagcta tcacagcagt tgctggtgcg ggagaagcta tcagtttctt tgggatccca
    12421 gtgattcttt caccatctgg ttatacttct tctgtcatac caatcattct agccgtatgg
    12481 gttcaaagta aattagagcc attcgtaaaa aaagtgattc cgcaatttct gcaaatgatt
    12541 ttagttccgc ttgtcgtttt agtagtgatg gttccattaa catttcttgc tttaggacca
    12601 atcgggacgg ttgccggtaa tgctttaggc ggtctcttca actcgattta tggatttagt
    12661 ccaatcgtag caggtctaat catgggaagt ttatggcaag tattcgtgat gttcggcatg
    12721 cattggggat tcgtaccgat catgtttttg aatatcgaac aatatgggtt tgatgtattg
    12781 atgccaatgc ttcttccagc catcttggca caaggtgggg ctgctttagc tgtagcattg
    12841 cgaacaaaag atacgaaact tcgagcatta gggatttttt ctacagtgac ttcgttattt
    12901 ggaatcactg aaccaactgt gtatggtgtg acattacctt tgaagaaacc attcattgcc
    12961 gcttgtattt ctggaggtat tggtggagct attatcggat tttccggagt aaaagctttc
    13021 tccagcagtt tggtcagctt attgacgatt ccgactttca ttaatacggt ggatggtgta
    13081 gagtcaaatg tgacagtagc agtaatagct acaggaattg cttttgttct tgcattcgta
    13141 ggtacattga ttttaggatt tgacgaacaa acacaagaca atcaattaga aaacaaacat
    13201 gcgaatgcag gagaaccgat tacttcagca cggcatacct taaaaagtcc tttaacgggt
    13261 aaagtactgc cattgagtga agtaccagac caagtttttt cttctggcgt gatgggaaaa
    13321 ggtatcgcta ttgatccaga agtaggagaa ctagttgcac ctgcagacgg agagatcacg
    13381 actatttttc caactggaca tgctgtaggg atcacaacga ctgacggagc ggagattttg
    13441 attcatatcg ggatggatac agtagaatta aatgggaatg gctttgagat cttagtcaaa
    13501 caaggggatc tcgtcaaagc aggagattta ttgattcgtt ttgacatcga agcaattaga
    13561 gcagctggct atagcgtaat cactccagtt gtcattacga atactgatgc atttgccgat
    13621 atacttgaat tagaccaaaa agagattatt gcaaacgaag atgttctggc aattgtaaaa
    13681 taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa tttttatggg gtggagcaac
    13741 tgctgccaaa tcaattagaa ggggcttatg atcgagacgg aaaaggacta tccgttgcag
    13801 atgctatgcc tggaggtaag cagcgctttg cgattatcgg cagtgaagaa tttgactgga
    13861 cgattgatca agaaaaatat atttacccga atcatcgagg aatcgatcat tacgatcgtt
    13921 tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa atgttatcgt ttttcgattg
    13981 cctggacacg aatttttcca aatggagacg aaggtactcc taacgaagcg ggattagaat
    14041 tttatgatca gctgattgat gagtgcttaa aatatgatat tgaacctgtg atcacgattt
    14101 ctcattacga aatgcctctt catttggcaa aagaatacgg tggctggaaa aatcgaaaac
    14161 tgatcgactt ctatgaacga tttgctcaga cagtattaga gagatacagc agtaaagtca
    14221 aatattggat gactttcaat gaaatcaatt cggcctttca ctttccagct ttaagtcaag
    14281 gattagtaaa aagcaatgga gcaggagagt accaaaatat tttccaagca tggcataatc
    14341 aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga attgcgttcg gatatccaaa
    14401 tcggctgcat gattatctat gcaacaactt acagcatcga tgccaatcca gtcaatcagg
    14461 cagcaacaat gatccaaaac caagaattca attttttctg cacggatgtc caagtacgtg
    14521 gagaataccc tgcatacaca gcgcgtaccc ataaaaaata tggagtcgat ccggaaaaat
    14581 tagagcaaac ggaaaaagac ttccagttgc taaaagaata tccagtggac tacatcggat
    14641 ttagttatta tatgtctacg gcaatcaatg agactgatcc acaagcagca acttcagaag
    14701 ggaacttgct gggaggagta aaaaatccgt ttcttgaggc aagtgaatgg ggctggcaga
    14761 tcgatccaga agggctgaga atcgcattga atgaattata caatcgttac cagaaaccat
    14821 tattcattgt tgaaaacggt cttggagcca ttgatcatat ggaagaggat ggcacgatta
    14881 ttgatgatta tcggatcgat tatttacgtc gtcatatcga agcgatggct aatgcagtag
    14941 cagacggtgt agatttgatg ggctatacgc cttggggatg tatcgatctt gtcagtgcat
    15001 caacaggaga aatgagtaaa cgttacggct ttatctatgt ggatctagac gatgaaggaa
    15061 atgggacact gaatcgatca aagaaaaaat cttttgattg gtataaagaa gtaattgcct
    15121 caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa agaacacact tctcacatgt
    15181 gatttgtgtt ctttttttat tttttatgtg ctattctttt agtaatagta aagcaaaggg
    15241 tgacgttatg aaaaggacac gtgtactata cttggaagtc gcagataaaa ttaaagaaga
    15301 tattttctca ggaaaatatc cagtcggttc gatgttgccg acagaaagtg aattagaaga
    15361 actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc gaactgctgg catcagatga
    15421 atatgtcgca aagaaaagtg gtcgtgggac gacggttcta agcaatcgtc cttacaatcg
    15481 attatccaaa gcggcaagct ttacgcaaat cttggagaac tcccgatatg aagtccggaa
    15541 agaaacgctg gatttgcaat tggtccatat aaagaaggaa gatcctttgt tttccttttt
    15601 cggtgagcaa gctgtttgtt ttaagcgctt atattatttg aacaatcagc cttatattta
    15661 ttttgttcat tatcttccac aagaaatgat gcaattcagc agagaagaat ttggcaaaga
    15721 atctttgtat cgtttgctga cgaaaaaagg atatactatt gactcatttt ctgatgattt
    15781 ctcggcagta tttttaacag aagaagaaca aggaattctg aaaacagacg cacagatcgg
    15841 catcaaacgg atacggaaat cgtctacgca aattggggaa atgattgaat attcggaggc
    15901 ggtctaccat acggagctgc atccttacca tattgattat gaaacataaa taaaaaaaga
    15961 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc
    16021 atatttttcg tttggttagg cttattaccg cagatacacg gctgtttctg gattaccatt
    16081 tattctggat aagggggtgt ggcacgattt tcagaaggaa tctatgaacc aatcactgta
    16141 ttgattttag cttcgatggt aaccccattc ggcttgacga tcgcttattt cttagggaaa
    16201 atcattcgta aaaatatact caatcgtcaa gaaatcgata cattgaagac cgcttttcct
    16261 atggggatct gtcagatcac agagggatgt ttccctatcg tattgaacga cttggcacgc
    16321 aatgtcatag caacgggagt cggtgggaca gtaggtggcg gattaagcat gttttggggc
    16381 gcagatagcc gtattccggc atcgggaatg tttgcagtcg ctacgatgac cagaccatgg
    16441 gcattcattg gtgcattgtt agctggttct tttgtcacag gaataaccat tttattattg
    16501 aaaaaaccag tagatccgaa tgcagaaatc atccaagaag aaaaagagga agaagacata
    16561 tcttgggatg atttgacaat tagttaggag aggcagaatg gattacaaga aaaaagtaca
    16621 atagatgcac cttgaggcag gaatggtatg aaagaatcag agttagaaag aatcgaatat
    16681 acagattttg gtttaggagc gatcgaaact gtccagagca tcggcaccct ccttgaaatg
    16741 acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg tctggtatac cttatgtcga
    16801 aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa aggaaagaaa gttattatac
    16861 agtaaaaaaa gaaattattc ttcatgctag agatcagtac acgttcgagc ccaatatcaa
    16921 acactagttc caggcaggcg caacaggtgc ggttatcact gaattttctt ccagcagtga
    16981 cgattcaagt gatattttta caaatccagc tatttatcgt taacaaaaag atttttagga
    17041 gaagctgatg aacaaaaatg atcaaatttt gatcgtttag ttcgtcggct tttttattta
    17101 gagaaacatc atttatcagt gaaacataac acgagaacaa aatgattttt tcgttaataa
    17161 aagaaa
//
LOCUS       DACDHJ010000041        17038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDHJ010000041 DACDHJ010000000
VERSION     DACDHJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.042"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(58..603)
                     /locus_tag="IV223_001057"
     CDS             complement(58..603)
                     /locus_tag="IV223_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8711360.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(615..1307)
                     /locus_tag="IV223_001058"
     CDS             complement(615..1307)
                     /locus_tag="IV223_001058"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8711361.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1326..1610)
                     /locus_tag="IV223_001059"
     CDS             complement(1326..1610)
                     /locus_tag="IV223_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711362.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1623..2186)
                     /locus_tag="IV223_001060"
     CDS             complement(1623..2186)
                     /locus_tag="IV223_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8711363.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2414..3076
                     /locus_tag="IV223_001061"
     CDS             2414..3076
                     /locus_tag="IV223_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8711364.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3200..4396
                     /locus_tag="IV223_001062"
     CDS             3200..4396
                     /locus_tag="IV223_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8711365.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4497..5873)
                     /locus_tag="IV223_001063"
     CDS             complement(4497..5873)
                     /locus_tag="IV223_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8711366.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5910..6776)
                     /locus_tag="IV223_001064"
     CDS             complement(5910..6776)
                     /locus_tag="IV223_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8711367.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6865..7890)
                     /locus_tag="IV223_001065"
     CDS             complement(6865..7890)
                     /locus_tag="IV223_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8711368.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7908..8582)
                     /locus_tag="IV223_001066"
     CDS             complement(7908..8582)
                     /locus_tag="IV223_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8711369.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8766..9659)
                     /locus_tag="IV223_001067"
     CDS             complement(8766..9659)
                     /locus_tag="IV223_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8711370.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9733..11430)
                     /locus_tag="IV223_001068"
     CDS             complement(9733..11430)
                     /locus_tag="IV223_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8711371.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11423..12736)
                     /locus_tag="IV223_001069"
     CDS             complement(11423..12736)
                     /locus_tag="IV223_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8711372.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12736..16254)
                     /locus_tag="IV223_001070"
     CDS             complement(12736..16254)
                     /locus_tag="IV223_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8711373.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16547..17005)
                     /locus_tag="IV223_001071"
     CDS             complement(16547..17005)
                     /locus_tag="IV223_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8711374.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4782 a         3835 c         2955 g         5466 t
ORIGIN      
        1 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
       61 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      121 atcgaaactt gccacggcat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      181 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      241 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      301 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      361 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      421 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      481 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      541 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      601 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      661 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      721 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      781 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      841 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      901 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
      961 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1021 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1081 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1141 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1201 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1261 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1321 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1381 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1441 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1501 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1561 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1621 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1681 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1741 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1801 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1861 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1921 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     1981 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2041 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2101 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2161 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2221 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2281 agattttctc taaatataga aaatccttct ttagtccgat actctttata gtagtccgta
     2341 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2401 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2461 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2521 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2581 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2641 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2701 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2761 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2821 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2881 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     2941 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3001 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3061 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3121 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3181 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3241 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3301 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3361 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3421 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3481 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3541 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3601 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3661 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3721 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3781 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3841 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3901 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     3961 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4021 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4081 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4141 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4201 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4261 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4321 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4381 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4441 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4501 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4561 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4621 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4681 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4741 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4801 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4861 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4921 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     4981 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5041 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5101 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5161 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5221 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5281 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5341 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5401 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5461 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5521 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5581 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5641 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5701 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5761 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5821 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5881 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     5941 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6001 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6061 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6121 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6181 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6241 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6301 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6361 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6421 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6481 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6541 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6601 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6661 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6721 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6781 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6841 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6901 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     6961 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7021 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7081 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7141 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7201 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7261 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7321 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7381 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7441 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7501 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7561 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7621 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7681 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7741 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7801 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7861 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7921 tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg
     7981 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8041 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8101 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8161 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8221 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8281 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8341 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8401 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8461 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8521 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8581 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8641 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8701 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8761 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8821 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8881 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     8941 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9001 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9061 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9121 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9181 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9241 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9301 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9361 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9421 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9481 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9541 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9601 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9661 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9721 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9781 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9841 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9901 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
     9961 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10021 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10081 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10141 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10201 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10261 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10321 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10381 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10441 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10501 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10561 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10621 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10681 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10741 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10801 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10861 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10921 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    10981 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11041 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11101 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11161 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11221 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11281 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11341 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11401 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11461 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11521 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11581 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11641 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11701 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11761 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11821 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11881 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    11941 cattagcagg agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa
    12001 aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga
    12061 agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt
    12121 cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct
    12181 gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa
    12241 ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc
    12301 cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg
    12361 tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag
    12421 gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa
    12481 atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct
    12541 cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc
    12601 gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca
    12661 tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac
    12721 acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata
    12781 ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga
    12841 tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat
    12901 tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc
    12961 ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac
    13021 ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg
    13081 aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa
    13141 gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat
    13201 ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg
    13261 gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa
    13321 ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc
    13381 ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac
    13441 ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat
    13501 agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg
    13561 agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc
    13621 agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc
    13681 accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa
    13741 cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag
    13801 atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta
    13861 aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct
    13921 ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga
    13981 atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc
    14041 ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga
    14101 aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc
    14161 aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg
    14221 gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc
    14281 ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt
    14341 cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc
    14401 gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt
    14461 ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct
    14521 gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc
    14581 cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata
    14641 taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa
    14701 attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt
    14761 gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa
    14821 atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc
    14881 ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt
    14941 cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg
    15001 ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga
    15061 tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg
    15121 gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt
    15181 gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa
    15241 tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa
    15301 accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata
    15361 agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt
    15421 attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc
    15481 ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac
    15541 tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa
    15601 aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt
    15661 agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc
    15721 cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt
    15781 ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa
    15841 tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga
    15901 actgatttgc tcatggttat tgatagctgt actattatgg taaaaattca ccataccgat
    15961 tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat
    16021 caacgcagca tttttagttt gactattggt cccagcggtt aattcactca tactcgctga
    16081 cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac
    16141 atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct
    16201 gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact
    16261 gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc
    16321 ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc
    16381 cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc
    16441 tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt
    16501 tgtaaacttc atctattctt tcatcaacca ccacttgaag aggtagttac ccttcgttca
    16561 catcggggat cttttctgtt tcaaaaatca atgtgccggt ttctgcttct tttgaataaa
    16621 cttttccgac ataccattct tgaccatcga tcgtctcgac ttttaggtct tctccatctt
    16681 catcatagga ggatagacct gttccacgaa cgttttttac ggtaccaatc ccggcggctg
    16741 ccgcatcgat tgctaaacgt attctacctt cattggcact ttcctttttt tcaaaagcca
    16801 gatgccattc aagggtttct tctttttcaa tatacttccc ttcaattttt gcatagtcct
    16861 catctaagaa tacctcatcc tcctttgttt ctgttgactc agcagctaca tccagggtcg
    16921 tcatatgata gactgactgg aaatatggtc caaatagcaa actgacagtt actaactgca
    16981 aaagcttcaa ttttaaccct ttcacatcct tatactcctt tcaaaaatat ttgtcttc
//
LOCUS       DACDHJ010000042        16697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDHJ010000042 DACDHJ010000000
VERSION     DACDHJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.043"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV223_001072"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IV223_001072"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8711375.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IV223_001073"
     CDS             complement(1172..1867)
                     /locus_tag="IV223_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8711376.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IV223_001074"
     CDS             complement(1880..2596)
                     /locus_tag="IV223_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8711377.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IV223_001075"
     CDS             2926..3534
                     /locus_tag="IV223_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8711378.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IV223_001076"
     CDS             3859..4182
                     /locus_tag="IV223_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8711379.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IV223_001077"
     CDS             4364..5734
                     /locus_tag="IV223_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8711380.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IV223_001078"
     CDS             complement(5864..7165)
                     /locus_tag="IV223_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8711381.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IV223_001079"
     CDS             complement(7519..9510)
                     /locus_tag="IV223_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8711382.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IV223_001080"
     CDS             complement(9654..9920)
                     /locus_tag="IV223_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8711383.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV223_001081"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IV223_001081"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8711384.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV223_001082"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IV223_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8711385.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IV223_001083"
     CDS             13264..13827
                     /locus_tag="IV223_001083"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8711386.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IV223_001084"
     CDS             complement(14080..14601)
                     /locus_tag="IV223_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8711387.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IV223_001085"
     CDS             complement(14604..15446)
                     /locus_tag="IV223_001085"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8711388.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IV223_001086"
     CDS             complement(15439..16164)
                     /locus_tag="IV223_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8711389.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IV223_001087"
     CDS             16340..16636
                     /locus_tag="IV223_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711390.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         4699 a         3489 c         3086 g         5423 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtatta
//
LOCUS       DACDHJ010000043        16195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDHJ010000043 DACDHJ010000000
VERSION     DACDHJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.044"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..612)
                     /locus_tag="IV223_001088"
     CDS             complement(<1..612)
                     /locus_tag="IV223_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711391.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLK"
     gene            complement(791..1510)
                     /locus_tag="IV223_001089"
     CDS             complement(791..1510)
                     /locus_tag="IV223_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8711392.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1641..2792)
                     /locus_tag="IV223_001090"
     CDS             complement(1641..2792)
                     /locus_tag="IV223_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8711393.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3009..3749)
                     /locus_tag="IV223_001091"
     CDS             complement(3009..3749)
                     /locus_tag="IV223_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8711394.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3753..4076)
                     /gene="rsfS"
                     /locus_tag="IV223_001092"
     CDS             complement(3753..4076)
                     /gene="rsfS"
                     /locus_tag="IV223_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8711395.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4133..4726)
                     /locus_tag="IV223_001093"
     CDS             complement(4133..4726)
                     /locus_tag="IV223_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8711396.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4710..5357)
                     /locus_tag="IV223_001094"
     CDS             complement(4710..5357)
                     /locus_tag="IV223_001094"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8711397.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5370..5681)
                     /gene="yhbY"
                     /locus_tag="IV223_001095"
     CDS             complement(5370..5681)
                     /gene="yhbY"
                     /locus_tag="IV223_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8711398.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5697..6806)
                     /gene="yqeH"
                     /locus_tag="IV223_001096"
     CDS             complement(5697..6806)
                     /gene="yqeH"
                     /locus_tag="IV223_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8711399.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6803..7330)
                     /locus_tag="IV223_001097"
     CDS             complement(6803..7330)
                     /locus_tag="IV223_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8711400.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7510..8295)
                     /locus_tag="IV223_001098"
     CDS             complement(7510..8295)
                     /locus_tag="IV223_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8711401.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8288..9157)
                     /locus_tag="IV223_001099"
     CDS             complement(8288..9157)
                     /locus_tag="IV223_001099"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8711402.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9158..10537)
                     /locus_tag="IV223_001100"
     CDS             complement(9158..10537)
                     /locus_tag="IV223_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8711403.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10545..10964)
                     /gene="fabZ"
                     /locus_tag="IV223_001101"
     CDS             complement(10545..10964)
                     /gene="fabZ"
                     /locus_tag="IV223_001101"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8711404.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10989..11465)
                     /gene="accB"
                     /locus_tag="IV223_001102"
     CDS             complement(10989..11465)
                     /gene="accB"
                     /locus_tag="IV223_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8711405.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11468..12703)
                     /gene="fabF"
                     /locus_tag="IV223_001103"
     CDS             complement(11468..12703)
                     /gene="fabF"
                     /locus_tag="IV223_001103"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8711406.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12722..13459)
                     /gene="fabG"
                     /locus_tag="IV223_001104"
     CDS             complement(12722..13459)
                     /gene="fabG"
                     /locus_tag="IV223_001104"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8711407.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13471..14388)
                     /gene="fabD"
                     /locus_tag="IV223_001105"
     CDS             complement(13471..14388)
                     /gene="fabD"
                     /locus_tag="IV223_001105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8711408.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14495..14719)
                     /locus_tag="IV223_001106"
     CDS             complement(14495..14719)
                     /locus_tag="IV223_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8711409.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14772..15734)
                     /locus_tag="IV223_001107"
     CDS             complement(14772..15734)
                     /locus_tag="IV223_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8711410.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15744..16184)
                     /locus_tag="IV223_001108"
     CDS             complement(15744..16184)
                     /locus_tag="IV223_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8711411.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4260 a         3701 c         2780 g         5454 t
ORIGIN      
        1 ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg gatattctgg
       61 gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt aaattcgatt
      121 ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc cacaatccaa
      181 aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt ttttcgtgtc
      241 tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc tccactctaa
      301 tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga
      361 acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg gtgcgcggtc
      421 caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc ctaatcgata
      481 gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag ttacgttcac
      541 agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat
      601 ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt tcacatgata
      661 cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac acaaaaaagc
      721 aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa
      781 aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat
      841 cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct
      901 cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta
      961 atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt
     1021 cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag
     1081 cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg
     1141 aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat
     1201 tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat
     1261 agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa
     1321 taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt
     1381 ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg
     1441 cataaatccg gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt
     1501 tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct
     1561 gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct
     1621 tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga
     1681 atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc
     1741 tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt
     1801 ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct
     1861 tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat
     1921 ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt
     1981 ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta
     2041 gaagataaca accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa
     2101 gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct
     2161 aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga
     2221 tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt
     2281 tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg
     2341 ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac
     2401 agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg
     2461 ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt
     2521 tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg
     2581 ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta
     2641 tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc
     2701 tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga
     2761 ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa
     2821 aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa
     2881 actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta
     2941 tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg
     3001 caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct
     3061 tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt
     3121 aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga
     3181 ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg
     3241 cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg atagctatat
     3301 tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt
     3361 tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata
     3421 tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg
     3481 agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca
     3541 atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag
     3601 gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt
     3661 aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg
     3721 taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact aagatcgact
     3781 agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa
     3841 acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca
     3901 atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg
     3961 ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca
     4021 tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca
     4081 atttttaatt gttctttact atctatgatg atttcctcct aatatgagca gcttattttt
     4141 ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa
     4201 gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg
     4261 caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta
     4321 cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg
     4381 catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa
     4441 gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga
     4501 ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa
     4561 tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta
     4621 ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt
     4681 tttctctaga ataagcagta tattcattac tatagtccat attcatacaa cccttcattt
     4741 tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc
     4801 ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga
     4861 tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc
     4921 agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag
     4981 taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt
     5041 ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct
     5101 aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt
     5161 ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca
     5221 gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc
     5281 tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc
     5341 ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt
     5401 tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg
     5461 atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta
     5521 ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg
     5581 atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct
     5641 ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa
     5701 atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact
     5761 ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca
     5821 gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt
     5881 aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga
     5941 atatttacat catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca
     6001 aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt
     6061 ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt
     6121 tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc
     6181 tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc
     6241 gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg
     6301 atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca
     6361 tgagatttct tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca
     6421 cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact
     6481 ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt
     6541 gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat
     6601 tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt ctcattataa
     6661 tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa
     6721 gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag
     6781 ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt
     6841 ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac
     6901 tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac
     6961 gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt
     7021 gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa
     7081 gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa
     7141 cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg
     7201 gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat
     7261 tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat
     7321 ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa
     7381 ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga
     7441 aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac
     7501 aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc
     7561 taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt
     7621 atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa
     7681 ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc
     7741 gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc
     7801 cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc
     7861 aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat
     7921 agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat
     7981 caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg
     8041 atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc
     8101 tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat
     8161 cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc
     8221 ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg
     8281 tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga
     8341 ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga
     8401 attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc
     8461 ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa
     8521 aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt
     8581 cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc
     8641 cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg
     8701 ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga
     8761 taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag
     8821 tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt
     8881 cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt
     8941 catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag
     9001 ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact
     9061 ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat
     9121 tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt
     9181 ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta
     9241 tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg
     9301 tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat
     9361 ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa
     9421 ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc
     9481 ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt
     9541 gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca
     9601 cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga
     9661 ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg
     9721 ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt
     9781 ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc
     9841 gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga
     9901 tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc
     9961 caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg
    10021 attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat
    10081 aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc
    10141 ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca
    10201 ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg
    10261 aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg
    10321 tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg
    10381 ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg
    10441 aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga
    10501 tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag
    10561 gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg
    10621 gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact
    10681 ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt
    10741 tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct
    10801 ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt
    10861 ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt
    10921 ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc
    10981 tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg
    11041 ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt
    11101 ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg
    11161 aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc
    11221 tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct
    11281 tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata
    11341 ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa
    11401 tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata
    11461 tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta
    11521 atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca
    11581 agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta
    11641 atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca
    11701 tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg
    11761 cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga
    11821 cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag
    11881 aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga
    11941 tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa
    12001 cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac
    12061 gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct
    12121 cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt
    12181 tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac
    12241 aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca
    12301 ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt
    12361 tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat
    12421 tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat
    12481 agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga
    12541 aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg
    12601 gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa
    12661 taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt
    12721 cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat
    12781 tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg
    12841 gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg
    12901 tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca
    12961 cagattttgt taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat
    13021 ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca
    13081 tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt
    13141 ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt
    13201 ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt
    13261 tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt
    13321 cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt
    13381 ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat
    13441 tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt
    13501 ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct
    13561 taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg
    13621 gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt
    13681 ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa
    13741 ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg
    13801 aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa
    13861 aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata
    13921 atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc
    13981 aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat
    14041 ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc
    14101 tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg
    14161 ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg
    14221 cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat
    14281 ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat
    14341 accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa
    14401 tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca
    14461 ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag
    14521 tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat
    14581 cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga
    14641 tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt
    14701 gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat
    14761 aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct
    14821 gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc
    14881 aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac
    14941 ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga
    15001 tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg
    15061 tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct
    15121 gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt
    15181 ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag
    15241 agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt
    15301 agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt
    15361 ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg
    15421 atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa
    15481 tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt
    15541 tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat
    15601 cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg
    15661 gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt
    15721 ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc
    15781 agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc
    15841 atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc
    15901 aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact
    15961 aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct
    16021 gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac
    16081 agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag
    16141 acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtc
//
LOCUS       DACDHJ010000044        15920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDHJ010000044 DACDHJ010000000
VERSION     DACDHJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.045"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IV223_001109"
     CDS             226..894
                     /locus_tag="IV223_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8711412.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IV223_001110"
     CDS             complement(1094..1480)
                     /locus_tag="IV223_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711413.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IV223_001111"
     CDS             complement(1452..2834)
                     /locus_tag="IV223_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8711414.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IV223_001112"
     CDS             complement(2990..4177)
                     /locus_tag="IV223_001112"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8711415.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IV223_001113"
     CDS             complement(4184..5215)
                     /locus_tag="IV223_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8711416.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IV223_001114"
     CDS             complement(5231..6685)
                     /locus_tag="IV223_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8711417.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6860..7765
                     /locus_tag="IV223_001115"
     CDS             6860..7765
                     /locus_tag="IV223_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8711418.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IV223_001116"
     CDS             7856..8533
                     /locus_tag="IV223_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8711419.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IV223_001117"
     CDS             8814..9710
                     /locus_tag="IV223_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8711420.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IV223_001118"
     CDS             complement(9765..11135)
                     /locus_tag="IV223_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8711421.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IV223_001119"
     CDS             11373..12119
                     /locus_tag="IV223_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8711422.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IV223_001120"
     CDS             12116..12955
                     /locus_tag="IV223_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8711423.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IV223_001121"
     CDS             12956..13906
                     /locus_tag="IV223_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8711424.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IV223_001122"
     CDS             14047..14799
                     /locus_tag="IV223_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711425.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..>15920
                     /gene="queG"
                     /locus_tag="IV223_001123"
     CDS             14818..>15920
                     /gene="queG"
                     /locus_tag="IV223_001123"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8711426.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVV"
BASE COUNT         5155 a         2989 c         3208 g         4568 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggatctactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagctat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat
//
LOCUS       DACDHJ010000045        15157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDHJ010000045 DACDHJ010000000
VERSION     DACDHJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.046"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV223_001124"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV223_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8711427.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV223_001125"
     CDS             complement(322..891)
                     /locus_tag="IV223_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8711428.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV223_001126"
     CDS             1077..1979
                     /locus_tag="IV223_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8711429.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV223_001127"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV223_001127"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8711430.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV223_001128"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV223_001128"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8711431.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV223_001129"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV223_001129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8711432.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV223_001130"
     CDS             8547..8732
                     /locus_tag="IV223_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8711433.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV223_001131"
     CDS             complement(8794..9990)
                     /locus_tag="IV223_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8711434.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV223_001132"
     CDS             complement(10160..11596)
                     /locus_tag="IV223_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8711435.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV223_001133"
     CDS             11889..12698
                     /locus_tag="IV223_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8711436.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IV223_001134"
     CDS             complement(12884..15037)
                     /locus_tag="IV223_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8711437.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4436 a         3209 c         2675 g         4837 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca agataaaatc aactttt
//
LOCUS       DACDHJ010000046        15104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDHJ010000046 DACDHJ010000000
VERSION     DACDHJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.047"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            256..512
                     /locus_tag="IV223_001135"
                     /pseudo
     CDS             256..512
                     /locus_tag="IV223_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            899..1126
                     /locus_tag="IV223_001136"
     CDS             899..1126
                     /locus_tag="IV223_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8711438.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1142..2308
                     /locus_tag="IV223_001137"
     CDS             1142..2308
                     /locus_tag="IV223_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8711439.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2543..3586
                     /gene="hrcA"
                     /locus_tag="IV223_001138"
     CDS             2543..3586
                     /gene="hrcA"
                     /locus_tag="IV223_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8711440.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3680..4243
                     /gene="grpE"
                     /locus_tag="IV223_001139"
     CDS             3680..4243
                     /gene="grpE"
                     /locus_tag="IV223_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8711441.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4294..6123
                     /gene="dnaK"
                     /locus_tag="IV223_001140"
     CDS             4294..6123
                     /gene="dnaK"
                     /locus_tag="IV223_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8711442.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6274..7440
                     /gene="dnaJ"
                     /locus_tag="IV223_001141"
     CDS             6274..7440
                     /gene="dnaJ"
                     /locus_tag="IV223_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8711443.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7808..8545)
                     /locus_tag="IV223_001142"
     CDS             complement(7808..8545)
                     /locus_tag="IV223_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8711444.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8547..10049)
                     /locus_tag="IV223_001143"
     CDS             complement(8547..10049)
                     /locus_tag="IV223_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8711445.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10308..12302
                     /gene="uvrB"
                     /locus_tag="IV223_001144"
     CDS             10308..12302
                     /gene="uvrB"
                     /locus_tag="IV223_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8711446.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12312..>15104
                     /gene="uvrA"
                     /locus_tag="IV223_001145"
     CDS             12312..>15104
                     /gene="uvrA"
                     /locus_tag="IV223_001145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8711447.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGH"
BASE COUNT         4948 a         2670 c         3316 g         4170 t
ORIGIN      
        1 tccttcctgt agtaatattt tttaaataac ctataggctt cttcaaaagt acatcctcca
       61 gtaaattcta agtactggag gattcttttt ctagtgttgc tttactcatg aaaaagtagt
      121 tggctacttc atcaaaattt ctgaactttt ttaatgtata ataaagagct cgatcattta
      181 ctacaaggat gctagctcct acattttcac agttttttag caagtaaatt tttctaaaat
      241 cacatcagga aatgcatgag attctttaag agaggcaatt atagggctat gattacggtt
      301 cgtgtaagtg acgccaaaaa agaatggctt tcttatatgg ctgaatttta tgggattact
      361 ttatctgaac ttctaaaaaa ctattcgatg gaacaactag aagatgaata caatcgtcaa
      421 acggctgaaa ttgcccataa acattggata agaagtggga aaacaacagt attcatggaa
      481 gaatccttaa tgaatttggt gggctggaat gacctgccgt ttaaaattca cgcccgaaat
      541 tcagaagcag ctctgattgg caaatacgtg tattggtaga gatagaagat aatcaattcg
      601 ttgttatcgc tattcaagta gggcaccgaa gaaatgtcta tgattaagag actgtttatt
      661 tttacaaaaa aataataatt ggaatctagt taaaactctt gaagtgagag atttgactag
      721 atttttttat gaataaaata aggacaaaat tgtttaggaa gaatcatgag tgtaaaaaaa
      781 tcatccaaga agtcgattct tggatttttt agtggatttg ttcgccaaat acgtttagta
      841 tgcgtatatg agaggttgta agcaaagcgt tgtggtctga gcacttaagg aggaatctgt
      901 gaaaatagct tttcatattt ttgcagattt tgacttaatg ccgaaggtca gctttgcgat
      961 taccgtttat cgagaggctg taaaagcacc gtgtagctcc gatgacaata agcaagcatt
     1021 tttagagcta ggtttccttg attctaaata tgcggcttat ttcaatgagt tggtgcttga
     1081 acgcgatttt aagaaggtcg aaaacaggca cgacttttcg acgtaaggag aaagaatttc
     1141 aatgatagaa acaataaatc ccgcagaaaa caaggtttct gcgtacattc atatcccatt
     1201 ttgcgagcat atctgctatt actgtgactt taataaagtt tttttagaag ggcagccagt
     1261 agatgagtat gtggaaatgc tgctgaatga aatgagatta atgctggaaa gtcatcctac
     1321 agcgaatttg gaaacacttt atgtaggagg cggaacacca acttctttat ctgctaaaca
     1381 acttgatcga ctattatcag gtgctagaga gatcctgcct tttcaaaatg gtaatgagtt
     1441 tactgtagaa gcgaatcctg gtgatttgac ccgtgaaaaa cttcaagtga tgaaaaacta
     1501 tggcgtcaat cgtttatcca tgggagtgca gacatttgac aatcgcttat tgaaaaaaat
     1561 tggacgtaaa catactgcag aagatgtgta tcaaacaatg caatttttag aagcagaaaa
     1621 ttttaccaat gtaagcatcg atttgattta tgcattaccg ggacaaacac tagaaggata
     1681 tcgggatact ttggaaaaag ctttggcttt ggatctgcct cactattctt tgtactcatt
     1741 gatcctcgaa aacaaaacaa tgttcatgaa ctgggtacga caaggaagac tcgaactgcc
     1801 tgatcaggaa acggaaacca gaatgtttga agagacgatt gaagcaatgg aaaagcatgg
     1861 acgtcaccaa tatgaaataa gtaactttgg gctctccgga catgaatctc aacataacct
     1921 catgtactgg aacaatgacc actattttgg gtttggagct ggtgctagcg gttatcttgg
     1981 agatattcgt taccgcaaca aaggaccaat ccaacactat ttacgcccat tacgagcaaa
     2041 tgaacgcccg gtattggaag aagaaaaatt gacacgaaaa aatcaaatag aagaagaaat
     2101 gttcttagga ctaagaaaaa aaacagggat tactaaaaaa catttctatg atcgctatca
     2161 aaaaacaatt gaatcggtgt acggtgatgt gctaagggaa ttagagcaag aagggttact
     2221 tgttaatgaa gaagaccgtg tttatttgac accaagaggg acttttttgg ggaacaatgt
     2281 gtttgagcgc tttttatttg aaaaatagtt tgaagaatat ttgtttttta tcgaaacttg
     2341 aaaagaaaaa gtttagaaag tttgacaatg aggtattgaa gtattgatat aactggattt
     2401 acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg gagtctttct
     2461 cttgacagga gaagggcttc ttgctatatt tataagtgaa ttagcactta gaatatatga
     2521 gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt tacgtctgat
     2581 catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattaa tgtctgaggg
     2641 agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag atgaaggatt
     2701 gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct atcgttatta
     2761 cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc tgattcgtca
     2821 gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg ctgagatatt
     2881 atctgagctg acaagctata cgactttttc attgggacct gaaataaaag accgccgact
     2941 gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg tcgtaacaga
     3001 tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa gtcaggaact
     3061 agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga tgacggtgta
     3121 ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaga cgacagaagg
     3181 gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagaaaagg tttatgtcag
     3241 tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt tcaaatcgat
     3301 gttttctttt atgaaagact cagacggact tacacaaatg atcactccga tggatagcaa
     3361 tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata tgagtatgat
     3421 tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt tagggcctac
     3481 tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag aactagcagc
     3541 taaacttgct gattactacc gctcgcttga cttgtcaaac tcgtaatgtg aataacgaag
     3601 ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc tgactgaata
     3661 atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag aaatgacaga
     3721 tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta tttcagaagc
     3781 tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa tggaagataa
     3841 atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa atgagcgtga
     3901 gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat caattgacaa
     3961 cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt tgaaaaaagg
     4021 cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa ttgaaaaaat
     4081 ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa ctgttccagc
     4141 gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct acaaattaca
     4201 tgatcgcgtg ttaagaccaa ctatggtcat tgttgcacaa taattgtgcg gatataaaac
     4261 ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg tattgactta
     4321 ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat catcgctaac
     4381 ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga aatccaagta
     4441 ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc aatcaaacgc
     4501 catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac accacaagaa
     4561 atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt aggtgaaaaa
     4621 gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg tcaagcaaca
     4681 aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga accaactgca
     4741 gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt agtattcgac
     4801 cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt ctttgatgta
     4861 ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa aatcatcgac
     4921 tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga caaaatggct
     4981 ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg cgtatcaagc
     5041 acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca tttggaaatg
     5101 acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg tacaaaagta
     5161 ccagtacgtc aagcgttaaa agatgcaggt ctgtcacaat cagaaatcga cgaagttatt
     5221 ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa agaaacagga
     5281 aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc tgctatccaa
     5341 ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac accattgtca
     5401 ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa tacaacgatt
     5461 ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc agtagacatc
     5521 catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg aagattccaa
     5581 ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac attcgatatt
     5641 gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa agaacaaaaa
     5701 atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat ggtgaaagat
     5761 gcagaagcaa acgctgaagc agataaagct cgtaaagagg aagttgacct acgtaatgat
     5821 gtagacgcat tgctattctc tgtggacaaa acattgaaag aattagaagg caaagtggac
     5881 gaagaagaag tgaaaaaagc agaaacagct cgtgacgaat taaaagccgc tgttgaagca
     5941 aacaacatcg aggaaatgaa aactaaacgc gatgcattga acgaaatcgt tcaaaacttg
     6001 actgttaaac tatacgaaca agctgcacaa caacaagctc aagaaaatcc agaagcagct
     6061 caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg cgacgataaa
     6121 taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt tttcttttga
     6181 aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata acaagcgacg
     6241 tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga ttattatgaa
     6301 gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta tcgtaagctt
     6361 tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa attcaaagaa
     6421 gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata cgaccaatac
     6481 gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg atttggtgga
     6541 ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga ctctttcttt
     6601 ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga tttgcaatat
     6661 acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat caagtataat
     6721 cgggaagaaa tctgtcatac ttgtggtgga aacggtgcaa aaccaggcac acaaccaact
     6781 acttgtcata aatgtcatgg tgcaggtacg atcaatgtag aacgccaaac gccacttgga
     6841 cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggca cagggaaaga aatcaaagaa
     6901 ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt taaagtgaat
     6961 gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg agaagctgga
     7021 gaaaacggcg gaccatttgg tgatttgtat gtggtttttc gtgtagaaga aagtgatatc
     7081 tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt acaagcagct
     7141 ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa gatccctgct
     7201 ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa gttacgtggt
     7261 ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa tctaaatgaa
     7321 caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt tgaagaacag
     7381 caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa gcggaagtag
     7441 tttttctagt aaaaatataa agtagacttg cccaaatttt ctatttctag taaaaacgct
     7501 aagtcattaa taattaaaaa ccagacgaac aatcatacgt tcgcctggtt tttttgtagt
     7561 tgtcatgttt gtaccaacaa aaaagccagc tttagaataa gataatcatc atagtttctg
     7621 aaggtggtat cgactatgta cttgatatcg tcaatgaacc gatggtactt tcttatgcga
     7681 tggaacaggt agagcgattg cttgtttgtg caggagaaca agcgatgcgg ctggtacagt
     7741 taagtgaaaa aacaagaaac taaaaatgcc cggtcgtcat gaagcgaccg ggcatttttg
     7801 gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
     7861 tttcaaagat ttgttgaggc gttccgtctt ctaaaaagtt tccgtcgtca atgaacatga
     7921 cacgattggc cacttcttta gcgaagccca tttcgtgcgt aacgataacc atcgtcatac
     7981 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttcg gggtcgagtg
     8041 cagatgtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
     8101 ccacacgctg cttttgtcca ccggaaagca tttcaggata catatctttt ttatcttcta
     8161 gaccaactgt ctgcagcaat ttgattgctt ttttctctgc ttcttctcta gattctttct
     8221 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
     8281 agtgctggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
     8341 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
     8401 aacgcaaaaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
     8461 cgttgatgga cacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
     8521 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
     8581 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
     8641 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
     8701 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
     8761 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
     8821 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
     8881 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
     8941 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
     9001 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
     9061 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
     9121 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
     9181 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
     9241 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
     9301 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
     9361 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
     9421 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
     9481 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
     9541 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
     9601 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
     9661 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
     9721 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
     9781 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
     9841 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
     9901 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
     9961 ttgtcagctt gtgcaacatg tccaaacgca agtgtcacaa gtcctgtcat taagaaaaca
    10021 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    10081 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    10141 cgagtttcta acattttatg ttataaaacg taacatgcat aataagataa aaaagtggcg
    10201 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    10261 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    10321 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    10381 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    10441 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    10501 cactaattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    10561 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    10621 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    10681 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    10741 catccgtttc atgtattttt ggattaggtt cgccttttga gtaccaaaaa caagtagttt
    10801 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    10861 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    10921 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    10981 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    11041 acgtggcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    11101 tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    11161 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    11221 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    11281 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    11341 cgcacgtaac catgcctcaa gtcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    11401 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    11461 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    11521 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    11581 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    11641 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    11701 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    11761 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    11821 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    11881 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    11941 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    12001 tgcgtctagc gatggatgaa acatcaagac gccgtacgat tcagcagaag tacaatgaag
    12061 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    12121 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtatgaagaa atgacgaaag
    12181 aagaaaaaga tacgttattg atgaaattag aaaaagaaat gaaaaatgcg gcgaaagcat
    12241 tggattttga aacagccgct aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    12301 aggaggacaa catggctaac gataaaatta cgattcacgg tgcacgtgcc cataacttga
    12361 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    12421 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    12481 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    12541 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    12601 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    12661 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    12721 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    12781 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    12841 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    12901 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    12961 ctcgtctatt tgattcttta gaggcggctc tgcgattggc agacggctat gctctggttg
    13021 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    13081 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    13141 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    13201 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    13261 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    13321 aagaattacc ggaagagcat caggatatca tcttgaatgg gacagatgat cttttccatt
    13381 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    13441 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    13501 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    13561 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    13621 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    13681 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    13741 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    13801 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    13861 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    13921 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatctga
    13981 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    14041 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    14101 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    14161 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    14221 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    14281 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    14341 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    14401 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    14461 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    14521 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    14581 atgttccttg tgaagtatgt catggcaaac gatataactc tgaaacatta gaagtgcatt
    14641 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaagatgct gtagaattct
    14701 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    14761 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    14821 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    14881 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    14941 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    15001 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    15061 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcac
//
LOCUS       DACDHJ010000047        14568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDHJ010000047 DACDHJ010000000
VERSION     DACDHJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.048"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..826
                     /locus_tag="IV223_001146"
     CDS             14..826
                     /locus_tag="IV223_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8711448.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            823..1623
                     /locus_tag="IV223_001147"
     CDS             823..1623
                     /locus_tag="IV223_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8711449.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1784..3052
                     /gene="rseP"
                     /locus_tag="IV223_001148"
     CDS             1784..3052
                     /gene="rseP"
                     /locus_tag="IV223_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8711450.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3120..4829
                     /locus_tag="IV223_001149"
     CDS             3120..4829
                     /locus_tag="IV223_001149"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8711451.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5041..9393
                     /locus_tag="IV223_001150"
     CDS             5041..9393
                     /locus_tag="IV223_001150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8711452.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9537..10010
                     /gene="rimP"
                     /locus_tag="IV223_001151"
     CDS             9537..10010
                     /gene="rimP"
                     /locus_tag="IV223_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8711453.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10034..11209
                     /gene="nusA"
                     /locus_tag="IV223_001152"
     CDS             10034..11209
                     /gene="nusA"
                     /locus_tag="IV223_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8711454.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11231..11524
                     /locus_tag="IV223_001153"
     CDS             11231..11524
                     /locus_tag="IV223_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8711455.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11521..11832
                     /locus_tag="IV223_001154"
     CDS             11521..11832
                     /locus_tag="IV223_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8711456.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11845..14151
                     /gene="infB"
                     /locus_tag="IV223_001155"
     CDS             11845..14151
                     /gene="infB"
                     /locus_tag="IV223_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8711457.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14178..14525
                     /gene="rbfA"
                     /locus_tag="IV223_001156"
     CDS             14178..14525
                     /gene="rbfA"
                     /locus_tag="IV223_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8711458.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4969 a         2576 c         3251 g         3772 t
ORIGIN      
        1 aggaggaaaa agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag
       61 cgaatttcaa tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg
      121 caatggtcgt tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat
      181 ggaaacagta aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct
      241 ttatgccttt tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca
      301 actacctgtc gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt
      361 tcatgtcatg ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc
      421 aattgagcaa acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag
      481 tagagcagag atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat
      541 acacgcagaa gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc
      601 taaagagtta caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa
      661 ttttctgtta tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga
      721 ttttgatggt gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt
      781 cggcggtgta aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc
      841 acagcagctg tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt
      901 gagcttacag cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga
      961 ttgacactct taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg
     1021 ccaaaagaaa gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat
     1081 cttatagtta tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat
     1141 gaagcaggtt ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc
     1201 aatgcacgaa cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact
     1261 gatatcggag catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt
     1321 tcgcccaaca aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca
     1381 tttctttatt tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg
     1441 cttctgatga cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca
     1501 atcaaacgtc attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc
     1561 ttagaccgtt tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt
     1621 tagtaagtat acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca
     1681 ttatattgat actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt
     1741 gttttataaa aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac
     1801 atttatcatc gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc
     1861 gaaacgatca ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg
     1921 gcatcaagca aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt
     1981 tcgaatggca ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct
     2041 tttactaaat tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac
     2101 aaatgctatc ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg
     2161 ttatgtgaat ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat
     2221 tattgaaaac gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc
     2281 taaattgtgg cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc
     2341 aatcgtgtta ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa
     2401 tcgcgtagga gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga
     2461 tgaggtagtc agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat
     2521 tacgaaaaat ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc
     2581 tgtcgacgtg acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat
     2641 aaaagcccca atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt
     2701 cagcggttca ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga
     2761 taaattaggc ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt
     2821 aacaactgtt attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct
     2881 tccgattcct gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg
     2941 gaaaccatta agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat
     3001 gttgttgatg gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt
     3061 gacaaatagg gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa
     3121 tgagacaatc aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat
     3181 taagccatca aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt
     3241 catatctgcc attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat
     3301 ttgaaaaaat cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg
     3361 aagaatccgg gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg
     3421 atcgaaaaat gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg
     3481 aaatcaattc atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg
     3541 acgaaaaacg acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat
     3601 attctttcca tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct
     3661 ataccgaagt attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg
     3721 caatgggcgg gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga
     3781 tctgttattc gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg
     3841 tatcaaaaaa atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa
     3901 aatcaattga cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg
     3961 tattgttcat tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca
     4021 atgacgtaaa actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag
     4081 aagcacaaaa atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg
     4141 acgtaaaagt atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga
     4201 acgaaacagg attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt
     4261 atgaagatct tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg
     4321 cctttactcg aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat
     4381 ctatgggagc aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt
     4441 atggtatcgg tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag
     4501 gaatcaattg gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa
     4561 aagacgaata ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct
     4621 atgaagtact ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt
     4681 tgattggctg tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag
     4741 tgaaaatcaa gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt
     4801 taccaatcct cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc
     4861 tgaatcagct gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa
     4921 tcgcagttcc aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga
     4981 aaaagaagag gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct
     5041 ttgtcagaaa aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca
     5101 gaaaaaaatc atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa
     5161 tcgcgtttat gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg
     5221 tcattgactc agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc
     5281 ttagcagatg accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg
     5341 gaaaatcagc aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg
     5401 ataaaagatc gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag
     5461 caacaatact tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt
     5521 atcgaaccag aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc
     5581 aaacaagaac aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa
     5641 caaaagaaaa aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga
     5701 cgaaatattc cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga
     5761 agagtcacac ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc
     5821 aagatcttga ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg
     5881 aacggcgaaa aagatgaaca agtatttgat gcaatcagtg tcggtagctg gttaaaggta
     5941 cgaggaagta ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc
     6001 atagaagtca aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag
     6061 cttcatgttc atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg
     6121 gcacaagcag gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa
     6181 gcctttccgg aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg
     6241 gaagcgaatg tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg
     6301 agtgaagcca catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac
     6361 acgatcatcg agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat
     6421 gaatttatcg atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg
     6481 gatgaaatgg ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt
     6541 tcaaaagata caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact
     6601 agctacgcaa ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta
     6661 gctagatatc tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt
     6721 ggtgttagct tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta
     6781 gcatggatct ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta
     6841 aatgagcata tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt
     6901 ttagcaaaaa atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc
     6961 gaatacttcg aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat
     7021 ctattgatcg gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg
     7081 gtagaagagg caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg
     7141 gtctatgcgc ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt
     7201 atccaaaatt tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat
     7261 gtacattatc tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc
     7321 ggagcgaatc cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag
     7381 atgctaactg catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat
     7441 acgaataaaa tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca
     7501 ccgaagattc caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa
     7561 atgtacggag atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct
     7621 attaatggta atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt
     7681 aatgaagatg gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg
     7741 atgacaggaa tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa
     7801 tattccgagt tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa
     7861 tgtccaaaat gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt
     7921 ttaggattcc atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa
     7981 gcagaagccc ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga
     8041 acgattggta cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac
     8101 aataatctgc agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc
     8161 aaacggacaa ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc
     8221 tatgatttta cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact
     8281 cattttgatt tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat
     8341 gacccaactg ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct
     8401 acggatgacc cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag
     8461 gaacaaattt attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt
     8521 cgagggatgt tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga
     8581 ctatctcatg gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat
     8641 gcgacactgg cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct
     8701 ggattagata gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac
     8761 aagatccctg atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc
     8821 gactggtata tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca
     8881 gcttacgtat tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat
     8941 tactgtgcat acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga
     9001 aaagatgctg tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta
     9061 aaagaaaaga atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc
     9121 aaatttggaa tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat
     9181 acgttgatcg caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc
     9241 gttgaagcaa gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa
     9301 gtttcaaaaa cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat
     9361 gaaaatcaat tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat
     9421 tttaagcaga gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt
     9481 tttcgctcac tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga
     9541 gtagcgtcgt tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg
     9601 aacttgtcga agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg
     9661 acaaagaagg cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa
     9721 aacttgatgc agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc
     9781 cgggagcaga gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg
     9841 tccatgtctc cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat
     9901 catttgatgc tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga
     9961 cttttgaccg aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag
    10021 gaatcagaaa aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa
    10081 agggatctca aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa
    10141 acgtcactat ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat
    10201 ccatgtctat gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc
    10261 attgaaagat gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga
    10321 agtaacacca aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca
    10381 gcgtgtacgt gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga
    10441 catcatgcaa ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa
    10501 aattgaggca gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga
    10561 tcgaatcaaa gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt
    10621 tagccgcagc catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta
    10681 tgatggaatc gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc
    10741 agttcgctca aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca
    10801 acgagttcaa gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa
    10861 tgaagatcca gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat
    10921 cttcgatgag acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct
    10981 agcaatcggt aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat
    11041 cgatatcaaa tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga
    11101 aacagaagaa ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga
    11161 aacaattgca ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg
    11221 aggcaaagag atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat
    11281 gaaacctaaa aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga
    11341 tccaactgga aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca
    11401 acaagcttgg gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt
    11461 ttatcaagaa cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa
    11521 atgaatacag ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga
    11581 aaactaatca cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg
    11641 gtgtttgtgg caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca
    11701 tattatgaag ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga
    11761 aaacctcgca aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta
    11821 attgaaggtt aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa
    11881 ctcaaaaaat ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa
    11941 aaccatatgg gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac
    12001 ccaagcacga aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg
    12061 aacgatcaac gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt
    12121 tatcaagatc gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac
    12181 caaaataata cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat
    12241 caaaaccgta ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat
    12301 aataaccgta atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa
    12361 ccagctgtac caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt
    12421 atgaacgtag cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa
    12481 ttatttatgc ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa
    12541 ctgttagcga cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat
    12601 atcgataaat tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca
    12661 gtagtaacga ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc
    12721 cattcacgtg taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag
    12781 atcgatattg atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca
    12841 agtatgcgtg cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat
    12901 gatggcgtca tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca
    12961 atcatcgtcg ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa
    13021 gaattaagtg agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag
    13081 atctcagcga aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca
    13141 gaagtagaag atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca
    13201 cgtcttgata aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac
    13261 gttggtgacc caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat
    13321 cttggccgtc gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat
    13381 gatgtacctc aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca
    13441 gcgggtgaag aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg
    13501 actttggaca acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc
    13561 atcaaagcag atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat
    13621 gtggaaggtg tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat
    13681 gtaaccttag cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg
    13741 caggcaaaag tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat
    13801 aaagccatcg aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa
    13861 aaaatcaccg gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt
    13921 gcaggtgcct atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt
    13981 gacggaatcg taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg
    14041 aaagaagtca aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa
    14101 gtagatgacg taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata
    14161 aaaggaggcc aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa
    14221 aagaagtcaa tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga
    14281 tcacagatgt gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat
    14341 ctgacttagc atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga
    14401 ttcgtcgaga gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac
    14461 ttgatgaatc agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag
    14521 actaatcaaa aaatcccgca aatcctgtga aatcaggggt tgcgggat
//
LOCUS       DACDHJ010000048        14500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDHJ010000048 DACDHJ010000000
VERSION     DACDHJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.049"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(360..616)
                     /locus_tag="IV223_001157"
                     /pseudo
     CDS             complement(360..616)
                     /locus_tag="IV223_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(803..1714)
                     /locus_tag="IV223_001158"
     CDS             complement(803..1714)
                     /locus_tag="IV223_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8711459.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(1733..2737)
                     /locus_tag="IV223_001159"
     CDS             complement(1733..2737)
                     /locus_tag="IV223_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8711460.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(2734..4857)
                     /locus_tag="IV223_001160"
     CDS             complement(2734..4857)
                     /locus_tag="IV223_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8711461.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(4862..7309)
                     /locus_tag="IV223_001161"
     CDS             complement(4862..7309)
                     /locus_tag="IV223_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8711462.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(7296..7685)
                     /locus_tag="IV223_001162"
     CDS             complement(7296..7685)
                     /locus_tag="IV223_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8711463.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(7745..8248)
                     /locus_tag="IV223_001163"
     CDS             complement(7745..8248)
                     /locus_tag="IV223_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8711464.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(8261..8485)
                     /locus_tag="IV223_001164"
     CDS             complement(8261..8485)
                     /locus_tag="IV223_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711465.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(8589..10499)
                     /gene="ltrA"
                     /locus_tag="IV223_001165"
     CDS             complement(8589..10499)
                     /gene="ltrA"
                     /locus_tag="IV223_001165"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8711466.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11245..11364)
                     /locus_tag="IV223_001166"
     CDS             complement(11245..11364)
                     /locus_tag="IV223_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8711467.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(11379..12563)
                     /locus_tag="IV223_001167"
     CDS             complement(11379..12563)
                     /locus_tag="IV223_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8711468.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(12822..13112)
                     /locus_tag="IV223_001168"
     CDS             complement(12822..13112)
                     /locus_tag="IV223_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711469.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(13102..14244)
                     /gene="dcm"
                     /locus_tag="IV223_001169"
                     /pseudo
     CDS             complement(13102..14244)
                     /gene="dcm"
                     /locus_tag="IV223_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT         4064 a         3201 c         2412 g         4823 t
ORIGIN      
        1 aaggtactgg tatcaattta agcacaataa aaaagctgaa aagtggtgaa agatcagtgg
       61 aaaagctgaa tcttttagat gctataaaaa taacggaatt tgctatgaaa aatggaaaag
      121 ctgagattga aatatggaga taattatcgt ctagtgaatt acttgccact tagctgaatt
      181 taacgatcct tggtgcttga agggggagat tcttgggaat agagcatact tgcaagccta
      241 tgtctttact aacaacgata tctcttatta accaagctat ccccgtagtt ctaaggcttt
      301 tacttttatc taagcaataa tcgtattcaa aggaaatttt tgacccttag aaagtctcat
      361 tttatcagac gtgagcttag gcattgctga attttgttaa ttcggtttta ggttgtgctt
      421 tgagcataat atgaaagtat tctttatcac gatttcattc atctaaggta atgtgatgtg
      481 attctgaaat tctttcaaag gcagtttttg cataagctga cattacatca tcaatgacat
      541 gtttacaatg tttcactact aaaataaggg ggtaaccaag aagaaaaact gaatgattat
      601 ttgtatctaa ttccatggta tatcaaccat ttcctatata gactgattat gacatggatt
      661 aaaataaaaa ggcactatgt cttaattgtc ggttttcgag ttaccgaatg gcattcatca
      721 cccacctata gaggatgggc gacttctgcc tgaaataagt tacactactt ataacttcta
      781 ataaaattag attgagtcat tattatttaa catcaacgat tttccagttt ccattttttt
      841 gtaatactaa tttgtactga aagtagctag tggtttttga tacattatca aggtatttta
      901 tctttgtact cactagaaaa ttttttccgt tttgtagata aactggattc tctaactccg
      961 aaaactttaa gtcaatattg attggtcgca tcgcaccatt ttcaacataa tactccaatt
     1021 ctttttcaga agctgatgga taaagtttaa aaaatgtttc taaaaaatcg gtgatttctt
     1081 ccgttgtttt ggcatcaatt tctgaatcgt tttctacttg ttttgcttca tattttgcct
     1141 tgtctggctt tccaatcatt gttggcaaac tagttactaa atgattcctg ttttcatctt
     1201 caaagattgt cactcgatat gcagaagaaa ttgattgcgt cttttttccc tctgtaatat
     1261 gttgctccac taaaaatgaa acgacaaatt cttccgagtg tttttcaaca tctagaattt
     1321 ttacagattg aacttcggaa ctcgtgggaa tatctgctcg aaccatatct tggattaaag
     1381 cgagaccgtt ttctgtaagg tactgttcga gattactcat tctcttttca attgcttctt
     1441 tgttattttt ccatgaaaaa tattctttcg caaaatcctc tgtaaaactt tcaacgccag
     1501 aagtattcac taccttctct tttataacca ctttttcatg agttgtatgt tgatcaatgg
     1561 cggtgaaatt tttataaacg ccaaaagcaa gactacaaga caataaaagc caaagggcaa
     1621 gcaccatttt tcgatgctta cccacggata caactcgttc ttttttcttt tttgggctct
     1681 tttccttttg attccgttca atttttatct tcattctttt tcctcttttc tcttatttgt
     1741 atcggccagc tccgataaga tgctgccgcc aatagttttc cgttagattc gtccacccca
     1801 ctggatctcc tgcatgaaac atgcgattct cccctgcgta aatgcccaca tgggtcacat
     1861 aagtaccggc atcataggtt gaatgaaaga aaactaaatc acctggtttg gcatctttta
     1921 gatcaatatg agtcatggca tcatattgat cctgagccac ccgaggaagt ttcaggtcaa
     1981 ctttggcata acaccattgg gtcaagccac tacaatcaaa ccccgtttct ggacttgagc
     2041 caccaaacac ataggccgtt ccttcatact taaatgcctc atcaaaaatt tcttggacta
     2101 ttttgtcatc aaactgagtt gtagttaggt attgcttcac taataaacag taaaacatat
     2161 tgccataatt ataccgccac ccaccatttt ccttaatcgc gattgggttg gggtaatcga
     2221 ctttttgacc acctgatttt tcctttgaaa aagattcagc taaagcaaaa ctatgcttct
     2281 tcccgttttt cccgacatga tctagatacc cgccaccata gttataggcc tgaataatac
     2341 tatcttgatc acaccctagt tctttggaac gcttaaccaa agacgcaaaa tagagacagc
     2401 cttgtttgat ggatgcctcg gtacttaatg aattaggtgg taatccggct gactcagaac
     2461 tttgcataac gtcttccatt gtgccaccag attccacttg aataatcgcc aaaagaattg
     2521 gaatatactc tgttatatcc gattctttgg catacttttc caccattgca cggtgtttca
     2581 atacttcttc tgagacacta gttccgccag gagaagtgcc agaactacca tttccctcac
     2641 tgtcttcacc aaacaacaaa cccacacaca tcaaaatacc cataaaagag atggtcagaa
     2701 aagttaatga aataacaccc actttcttca agttcatttt ttctcacctg accttgattt
     2761 tggacgatgg ttcattcttt tttgtacttt ttgattggtc tgctttggca gagtggacct
     2821 ctgtttcact tgttggaaag aatttttctt acttgctgat tggcttttcc cttttgaaat
     2881 gatttcttga ctagttagtt ttcgtttgat ttcgggattc agatttgctc tcacaatccg
     2941 ctttggtgct tcttttttca gcatcatatc tttctgaggt tgagctgctt tatctattgg
     3001 aatattcgcc cttctttttc cctgacgatt tgttgcttca ctcagtatct tgcgtttttc
     3061 gtccattttc gcatttcttc gttgtcgttg tttttctcgc aaatccactc gatccgcttt
     3121 ttcttggaac agtccgcgct tgaattcttc tggtgctttt ttcgactttt cgattccttt
     3181 atggagattg tatttcaaat tcgttggcgt attccttacc tgatccttta cttgtttagt
     3241 cttatctttc accaaagctt gggtatccaa aactttgccg atttttctcc cagccaaatt
     3301 cattcgggat tgtttcttat tatttgaaac tggattctcc ttagttaagt cagatagtat
     3361 ttcgtgatcg ttcggtaatc gtgaattctt tcgtagagaa gaaccggatg gcttaaattt
     3421 ggattttgat tgttcctttg ccaacttatt tccgactgct gcaccaccac caaaagccaa
     3481 agctcgtccc acatttcgat tgagttgccg caattttcga ttcattaaca tttgcggttt
     3541 tcgcatgacg cgtcttccca ttgactggct atcattactc tggaggttga acatactcat
     3601 aatgtcgccc aatttgaaat agataccggc aaaggtcaca atttgcagaa aagcaaccat
     3661 gaaaaatgga taggttgccg aaatattgaa aaacatcgtg gagatgctaa atgcagtcgt
     3721 aatcaccaaa gtaacacccg ctcgcatcat aatcgtattg aataaacgaa taatcgcctt
     3781 ctttcctaaa ctttcataag tcggcaacat ggacaataag aaactaattg gcagaaacat
     3841 cgcaaagata atgaagagaa tttgcgagaa caacataatc cctgttagaa gaaaaacaaa
     3901 gaaggaaatg ccaatattga acaataccaa aaagaccacg gtccctaaac ggttcatggt
     3961 cttggtaata ctgagattcg cattgtcatt gtcttcaatc tctgccttga ccgcttcttc
     4021 ccgatctttg cctttgtttt catctggact cacagataga atcttgttga cccggtcttc
     4081 accaatttct tctatgttgg aatcatcaaa ttgcagtagt aaccaaggtt gttggacttg
     4141 aatcgaaaac aaactatccc gaatcaaatc cacactatct tttccttggc tttctgaatt
     4201 gggtacaacg atctcggttc caagggtaag ggccgcttca ctaacatctg agctaaagtc
     4261 attgattttt gtgatatagg taggcgcata agcaatgaac gaacccgata gtagaaaaat
     4321 catcacaaaa ttaatcacgg ctcgaactgc tttcgtggtt tctcgtttta ataatccggt
     4381 atacgccaca tacaccccca gagctaaaat catcaataat agaaacccaa aataaaatcc
     4441 ggaggtctgg agtccatttt cagtaatacc agccaatgtt tggatattct ttccaatact
     4501 ttcagccgta tccgaaatga aatctaattt gtaagcctct tgaaccacat aacccgtggc
     4561 gttcgacaaa tacaaactca ccgtccaaat gaaattagta atggcataaa gaccatacat
     4621 cacgcttttc cctaagccat caccccaatt ccaaggcaac caatcccaag agctatccac
     4681 aaagaaatct agttgatagt tgttcagtga atatttggaa tatagatttc ctgcttcaac
     4741 tgtatcatca actagccctg tcgcctcggc taccgtacca actaaactca atagaactat
     4801 cagagtgagt atgctaataa aaacgattcc cacaatccga aaaagtttct ttttcatccc
     4861 ctcactccat tctctcttga acaggtggac gggtatcaaa ggcatggaag agttcttcaa
     4921 agactggatg tacctgaacc acgcctaccc gaccatacaa atcctgcatc aagcattggc
     4981 cattttccaa atctcgtagg cgtttttgat tgctttcatc ttctcgatcc aagccaaaga
     5041 attccaaggt tttcttgatt tcatgaatat cggtactgcg aaacgcaaac ttcaacccga
     5101 tattattctt caagcgttca tccagtaaat catcactatt ttgcgtgaca aagtaaaccc
     5161 cggcattcat agcccgccca gcacgaacga gcttattcga taatgttttc ccttgcgcta
     5221 cttgaagaaa actccacgcc tcatctaagt ctacaatttt gaagatttct cgattactat
     5281 ggataaagtc caaggcaaaa gtggaaatga cgattagcat ggaaacacta agcaattcca
     5341 tggtggtata ttcttcaaaa gtggtttctg catctggtaa aaccaaatcc gctacttgaa
     5401 tgatatttaa ctgcctatct aaactaatcg actgggtaat cgaaccatct gagaaaagca
     5461 aatgggcaaa atcataatcg gtaaaggact cgatgtgatc cgcgatattt tccgcaactg
     5521 gtgagccttc ttttcgaagc tcattaatta cttgtaataa tccccgttga ttgctttggg
     5581 taaccgtccg aatggcctta cgcaacactg gaaacttctc cccatctcga ctagaaattc
     5641 ctgttaaaaa ggtcagaata tcaattgcta gactttcaga atccttcttt ttctccataa
     5701 tcacataagg atcaagtaat ccttgatttt ttggcttact agttaagttg agaatattga
     5761 tttcatccgc aatttctggc aaggtttctt tccagccacc ccgttcccct tttggatcga
     5821 caatcacagc ttgtccacca aaaaggacgg cgtaataaac taagagattg ttactaaagg
     5881 actttccgcc accgagtgaa ccgaggaatg ccgctgctag cgcattggta actgaaccct
     5941 tgaccccttg tgaggccaat gccggtttca ggtaaacatt gcgccccgta tctacgttat
     6001 aaccaaagta aatgccttcc aactccccta acatctgagt ggcgccaaaa ccaagtccgg
     6061 cgagaaaatc agacgtaaca tactggatat aatcattcat gtatcgctta ctagaaggta
     6121 agaattcttc atgtagcccc atcatatcgc caaaaggccg gactagtttt acattggtat
     6181 catcataaaa atctagcact tcatcacacc ggcgctttag ttcatctact gattcggcac
     6241 ttactcgaac cacatagctc agtttgtaca tggattcttt ggactgatcc aaggtagttt
     6301 ccaattcatc tacggaatcc aatgcatcta agacattgga atttgtttca ttgtcagatt
     6361 gataggcatg gttgtccaag tcttttaatt cctttttctt attgcgaacg gtagcaagtg
     6421 cctttttgtt cgtcacaatc tccacattca tactagtgtc aattgggaat gtaaattgtt
     6481 gttgctggta atagaaaagt tctgaggagg gaaactccat ttcccccaca attgtattaa
     6541 ttgttaagta cgctacatat gattcatgat tctcatgttc catgcataag taccggggct
     6601 tttcttcaat caaacaacga ctgggacgta ataagtcgta acgtttcacc aaagtttccg
     6661 acttcagctt tttctttggt agctgaaatt catattcttc aaatggtgta cccttttcgc
     6721 catagatatg ttcaatcaaa tacatcagat cactcggtgt gactcgtctg actttaaatc
     6781 ttcgagccaa tttactttcc attagttttt caagcttggt atatcggcga atttcttcat
     6841 tggataagga aacaaaatcg cccatcaaat gatgattcac tccatagaca aattcttgaa
     6901 agccggagaa gaaagatttc ttgagattct ttagattgac ctcttcatct gtcgcaatca
     6961 gtttgaagcc aataaaaaag cgataatcaa tctgactatc gccaatcatg gatactaatg
     7021 cctctgtctg taaatcaatt cgttgtttcg ctacttctgt taatcgcccg gtaatttcct
     7081 tttttgatcg ttcttgcgtg gcacgaatac tactttctgt ggcaatttgt aaggcatgaa
     7141 tcttgccttc acgattttgc gcaatcaatt gacgaaagtt atcgtggact tgatatttct
     7201 gttcaggtga caaaaaggag taattgtaag gtattaactc atagtaggca aaacattctc
     7261 cctcttgatt aaagacaaga ttattttcaa tatacttaat cgggtacata gttcacactc
     7321 cttaccagag taatgggttc gtcccatcgt ttcttctgtt ctttgacttt ctttcctgcg
     7381 aaagtgacct taaatcgtaa gtgatagcta ataaaagatc gtaaaaatcc caatggtttc
     7441 ttaccgtcaa acgttttttg cgacatgaac caagtaactg ccaccggtat acctaagtat
     7501 tttaaaaagg ccccctctat catagatagt ggaggcacat gggcaaatac gatgacaaac
     7561 agcaaggata aaacaaacca agccatctgg ttaaaagtca caggaaaagg cagttgaaag
     7621 tcattaattg cataaatgac cttttcaact gcccaaatac tggtgtagct ttttattttc
     7681 ttcatcaaat cattccttta aaaagaagga tagtttagaa atcaactacc cttcaataat
     7741 tttgttaata tggatactca aaaacacctc gactcgttac caaataattg cctccaatct
     7801 ccaagtctct accacacgca tcaaaatcaa tatacgtctt caagtgttct ggtacttctc
     7861 caaaatcacc agcttctaca atcaagaaat atgctacatc actcatatca tcgcaatctg
     7921 gataatagat aatatcatcc acatgctcta ccatctcctc aaaagaattg aagaaagcat
     7981 gttgaatctc agaagcaact tcaccaactg gcgttccttc aagttcctcc gctaagccac
     8041 acaaccgatt aatttcctca attggcgtat actcatcaat ctcaaaggga agttcataat
     8101 catgaattgc atattcctcg tattcgtcat ttaacccaat tcgttctttc acttcatcaa
     8161 aatcgacagg tggggtaaac cacgccccca ccaattcacc ttcgttatat ttcccgaggt
     8221 tcgcaatata aacacgcatc tgttccatcg tgcttccccc ttacgcaccg acaattcggt
     8281 taaacaaact gagtaagaca tccttcaccc cagaagcatt aaagaccaat ccgaccgcaa
     8341 tcaaagcaat caccaaaaag ccaatcagtt tggaaaactc ccgcttaaat cctaaaaaca
     8401 aaccaatgac cacaattgcc agtaagacta aggattgggc attggttaaa aaccattgat
     8461 atatggatag gtaagttcct gatattataa atatctcaaa cttttccccc cattcacacg
     8521 gagcgtgcga ctttcaccgc actccgcgtt ccatcgtgtt tatattgaaa agtatttatg
     8581 tagtagacct atatcaagtt taattcttga ttgccagcta atactctata tttatttagt
     8641 cttatgagct ggactttatt tagcttcaat agagctatat atttttgtat tgtttcttct
     8701 gttttagcgt gtattagacg gtgtaccgcc tcttttacca aaataagatt tttataatcg
     8761 tcatttccac cagctccagt tggtacaata tgatgacaat gtatttcatt agcttgttga
     8821 aattcttcca gagttatagc acattttcca tgttgagcag aaaatagcga aattcgattg
     8881 tctgcgtatt ctattgtgcg attttccaaa gataatttca ttaactggta catcagtaat
     8941 ttatcaacac ttagattggt gtggataaat tttcttccct ctagagtgta gcaattcgct
     9001 aaatacacct ttgccattgg ttttctgtgt tggatatatc caacagggaa aataggttcg
     9061 ccctcgccag caacataccg aagcatttta gatttaccat atcttgttgc ctcctttgtc
     9121 gtaagttgtc tgcctgtttt tctcaatctg ccccttttgt ttgtacctaa tctgttagtg
     9181 aagatagcca ttacagctct attcagtttc atacaatcca gatttacatt cgttgcaatc
     9241 tgataataat tttgcatacc taataccatt gaattataaa gtcttgtttc ttctaattcc
     9301 gttttccctt ttgagggaga agcaattttc tttgcctgtg ataccagctt gtctttttgg
     9361 tttttgaatt gtttatcact tatatgtgat ttaacaacta acttctttct tctcggtatc
     9421 attttaatct tgaaaccaag aaagtcggag tatctttttc taacatttac aattcttgtt
     9481 ttttcttgtg atacttccaa ttttaatcgt tgttcaatcc attgagtgat tgctattttg
     9541 gctttttcag cactttcctt gtagcggcag aaaactctaa aatcatctgc ataacgaacc
     9601 atatacattt cctttaactt tgttttcttc atttctttgt aagcgttgct tttacatggg
     9661 ctaccgcttt tattgatatg aaccacatag ttttttgtaa caggattttc ctgccattgg
     9721 ctttctaccc aatggtctaa ctcattaaga acaatgtttg ctagtagtgg ggatattatt
     9781 cccccttgcg gagttccttt ctctggatat gttattgtac cgttagtcat tttgattgga
     9841 gcttttaata tcctttttaa aatgaatatc aagtgcttgt cccgtattcc catagcccat
     9901 atctgtttta tcagcttgga atggttcacg ttgtcaaaga aacccttaat atcaaattca
     9961 atcacataat gtagctggct gatttgtagg cggttatagg ttgcttttat tgcattttca
    10021 actgacctat taggtctaaa accatagctg ttttcactaa atttagcttc gcagattggt
    10081 tctaatactt gttttataca ttgttgtata agcctgtccc acatacaagg aatacctaga
    10141 ggtctggttt taccatttgg ttttggtatt tcttttcgcc tcactggctt aggtcgatac
    10201 ccatgtggac tgcctgcaac aatgtatctt acttttttga caactgtttc tgctggcaat
    10261 ttgccaatat ctttaattat tgtaccgtct gttcctgctg ttttactgcc tgcatttgct
    10321 ttgatatttc gatatgccag taatatattt tctctcgata ggataatatc cataagatga
    10381 gtgaaaatat ctccattctt acttttggca tataaatcat caaaggtgtt ttgcatttcg
    10441 taatattcag cgtgtcttaa atcttctaca cacaatgttt catttttatt tttaggcata
    10501 aggcttcact cctttacttg aaattgagtg agctttttta tcttactcgt aatccttatt
    10561 gaaacaacat aatacttttc taatatatac acgacttgag ggcattcctc cattctcatt
    10621 acaagaattt cttaggttcg ccctctgctt ttcagcatta gttcaactgc ttattgttct
    10681 tcgtcagttt attctcttga tgagaaccca atacctttcc tcgttccgat aatcttatct
    10741 atacatatat atccttaggt cactagctat aagcctgctg gcttgatacc gcctgtaacg
    10801 ttatatggca tttcataacc tatattctta ctttgccaca ccagctacga tttctcgtac
    10861 ctttgcattt ctacaaagtt caaatttaga cccgtacatt cgctgtttcg tcagttcctt
    10921 atagaacatt ctaaccatag atattttata gacccccgac ctgttggata cgctacccac
    10981 ctctggatag ttttcgatag aagatatatg ttcatcaact attacggtat ctttcagccg
    11041 tctttaccga gcttcaaaca ttctgagttt cctcatacaa tgcttgtcgg aggctcaggg
    11101 caggcgtttc agagcgttac ttcatcattc cacctgtcag attatcagtt ctcctaataa
    11161 ccaaactttt tcgttctttt attagtgcct tagcttttca ctaaggaacg agtcgcacat
    11221 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
    11281 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
    11341 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
    11401 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
    11461 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
    11521 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
    11581 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
    11641 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
    11701 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
    11761 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
    11821 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
    11881 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
    11941 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
    12001 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
    12061 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
    12121 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
    12181 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
    12241 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
    12301 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
    12361 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
    12421 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
    12481 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
    12541 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
    12601 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
    12661 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
    12721 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
    12781 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
    12841 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
    12901 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
    12961 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
    13021 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
    13081 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
    13141 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
    13201 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
    13261 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
    13321 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
    13381 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
    13441 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
    13501 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
    13561 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
    13621 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
    13681 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
    13741 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
    13801 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
    13861 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
    13921 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
    13981 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
    14041 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
    14101 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
    14161 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
    14221 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
    14281 acattttgct cttggctcat acattattaa tttgtcctac tattttttat ttaatccaac
    14341 taatttccgt tgtttttcat tggcctgatc ggctacatat ttaatcaccg ttttgaactc
    14401 ttttgcagta gcattttccg ataaatccgc aatgctctta ttgtatttct ttacaatttt
    14461 cgttacttgt tctttctcag tcatgtaatt gaacctccaa
//
LOCUS       DACDHJ010000049        14498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDHJ010000049 DACDHJ010000000
VERSION     DACDHJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.050"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IV223_001170"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IV223_001170"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8711470.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IV223_001171"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IV223_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8711471.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IV223_001172"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IV223_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8711472.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IV223_001173"
     CDS             2857..3732
                     /locus_tag="IV223_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8711473.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV223_001174"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV223_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8711474.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IV223_001175"
     CDS             4629..5063
                     /locus_tag="IV223_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8711475.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IV223_001176"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IV223_001176"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8711476.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IV223_001177"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IV223_001177"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8711477.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="IV223_001178"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="IV223_001178"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8711478.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="IV223_001179"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="IV223_001179"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8711479.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="IV223_001180"
     CDS             13334..14260
                     /locus_tag="IV223_001180"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8711480.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         5101 a         2508 c         3139 g         3750 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccg
//
LOCUS       DACDHJ010000050        14146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDHJ010000050 DACDHJ010000000
VERSION     DACDHJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.051"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..330)
                     /locus_tag="IV223_001181"
     CDS             complement(1..330)
                     /locus_tag="IV223_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8711481.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="IV223_001182"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="IV223_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8711482.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="IV223_001183"
     CDS             1251..2264
                     /locus_tag="IV223_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8711483.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="IV223_001184"
     CDS             2264..3541
                     /locus_tag="IV223_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8711484.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..6306
                     /locus_tag="IV223_001185"
     CDS             3604..6306
                     /locus_tag="IV223_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8711485.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6458..6775
                     /locus_tag="IV223_001186"
     CDS             6458..6775
                     /locus_tag="IV223_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8711486.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6805..7125
                     /locus_tag="IV223_001187"
     CDS             6805..7125
                     /locus_tag="IV223_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8711487.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7233..8693
                     /locus_tag="IV223_001188"
     CDS             7233..8693
                     /locus_tag="IV223_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8711488.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8761..8982
                     /locus_tag="IV223_001189"
     CDS             8761..8982
                     /locus_tag="IV223_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711489.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9013..9195
                     /locus_tag="IV223_001190"
     CDS             9013..9195
                     /locus_tag="IV223_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8711490.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9195..9608
                     /locus_tag="IV223_001191"
     CDS             9195..9608
                     /locus_tag="IV223_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8711491.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9731..10912
                     /locus_tag="IV223_001192"
     CDS             9731..10912
                     /locus_tag="IV223_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8711492.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            11301..11374
                     /locus_tag="IV223_001193"
     tRNA            11301..11374
                     /locus_tag="IV223_001193"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11335..11337,aa:Arg,seq:cct)
     gene            complement(11443..12582)
                     /locus_tag="IV223_001194"
     CDS             complement(11443..12582)
                     /locus_tag="IV223_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8711493.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12881..13516)
                     /locus_tag="IV223_001195"
     CDS             complement(12881..13516)
                     /locus_tag="IV223_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711494.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13629..>14146)
                     /locus_tag="IV223_001196"
     CDS             complement(13629..>14146)
                     /locus_tag="IV223_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP8711495.1"
                     /translation="IIFTTVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQF
                     EEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIA
                     DKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQ
                     WDMINSKVSKK"
BASE COUNT         4527 a         2392 c         2832 g         4395 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
     5341 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
     5401 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
     5461 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
     5521 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
     5581 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
     5641 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
     5701 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
     5761 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
     5821 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
     5881 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
     5941 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
     6001 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
     6061 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
     6121 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
     6181 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
     6241 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
     6301 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
     6361 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
     6421 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
     6481 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
     6541 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
     6601 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
     6661 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
     6721 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
     6781 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
     6841 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
     6901 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
     6961 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
     7021 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
     7081 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
     7141 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
     7201 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
     7261 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
     7321 atgcttttat cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
     7381 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
     7441 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
     7501 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
     7561 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
     7621 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
     7681 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
     7741 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
     7801 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
     7861 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
     7921 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
     7981 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
     8041 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
     8101 gaccaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
     8161 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
     8221 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
     8281 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
     8341 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
     8401 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
     8461 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
     8521 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
     8581 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
     8641 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
     8701 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
     8761 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
     8821 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
     8881 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
     8941 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
     9001 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
     9061 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
     9121 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
     9181 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
     9241 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
     9301 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
     9361 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
     9421 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
     9481 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
     9541 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
     9601 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
     9661 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
     9721 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
     9781 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
     9841 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
     9901 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
     9961 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    10021 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    10081 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    10141 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    10201 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    10261 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    10321 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    10381 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    10441 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    10501 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    10561 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    10621 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    10681 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    10741 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    10801 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    10861 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    10921 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    10981 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    11041 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    11101 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    11161 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    11221 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    11281 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    11341 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    11401 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    11461 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    11521 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    11581 aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa
    11641 agtaaattgc ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc
    11701 gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat
    11761 ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt
    11821 atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt
    11881 taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc
    11941 ccatgttaat cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag
    12001 cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa
    12061 ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac
    12121 tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc
    12181 tctaatatgg ttattaaact taataacggt agattttaca tggtctttag catacttatt
    12241 taaaaagcgt tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt
    12301 ctttatgttc attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc
    12361 tttataaagt tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc
    12421 tttaaggcct ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt
    12481 tgtcttaaat cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat
    12541 ttcgtattgc caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt
    12601 tctctgatac aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac
    12661 atcctttttg cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat
    12721 gtgtattaga tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata
    12781 actgaacttc cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt
    12841 gcattgctga taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa
    12901 taaattgaat tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta
    12961 atactatgga gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac
    13021 catctattag ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc
    13081 cacgactcat taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa
    13141 gaagataata ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat
    13201 aaagattttt atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta
    13261 tcccttcgaa tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa
    13321 tttgatcgcg aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt
    13381 tgaaacgctt ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag
    13441 gaattgctcc aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt
    13501 cattgctatt atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc
    13561 ctgatggcac tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg
    13621 tggttcattt attttttact tactttacta ttaatcatat cccattgagc ataggatttt
    13681 tctatgttcg atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta
    13741 aaggtatcag cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca
    13801 gaaatatcat tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta
    13861 gtcattgatt cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta
    13921 aaagctgtat tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc
    13981 cagtcatgat tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg
    14041 ttagatgtag tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc
    14101 tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa taatcc
//
LOCUS       DACDHJ010000051        13709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDHJ010000051 DACDHJ010000000
VERSION     DACDHJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.052"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(86..334)
                     /locus_tag="IV223_001197"
     CDS             complement(86..334)
                     /locus_tag="IV223_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711496.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            333..461
                     /locus_tag="IV223_001198"
     CDS             333..461
                     /locus_tag="IV223_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8711497.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            518..2212
                     /locus_tag="IV223_001199"
     CDS             518..2212
                     /locus_tag="IV223_001199"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8711498.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            2312..2485
                     /locus_tag="IV223_001200"
     CDS             2312..2485
                     /locus_tag="IV223_001200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711499.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            2863..3996
                     /locus_tag="IV223_001201"
     CDS             2863..3996
                     /locus_tag="IV223_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8711500.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            4027..4347
                     /locus_tag="IV223_001202"
     CDS             4027..4347
                     /locus_tag="IV223_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711501.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(4376..4840)
                     /locus_tag="IV223_001203"
     CDS             complement(4376..4840)
                     /locus_tag="IV223_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8711502.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(4972..6225)
                     /locus_tag="IV223_001204"
     CDS             complement(4972..6225)
                     /locus_tag="IV223_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8711503.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(6254..6571)
                     /locus_tag="IV223_001205"
     CDS             complement(6254..6571)
                     /locus_tag="IV223_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8711504.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(6597..6917)
                     /locus_tag="IV223_001206"
     CDS             complement(6597..6917)
                     /locus_tag="IV223_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8711505.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLEQAEK
                     LINK"
     gene            complement(7381..8796)
                     /locus_tag="IV223_001207"
     CDS             complement(7381..8796)
                     /locus_tag="IV223_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8711506.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(8813..10129)
                     /locus_tag="IV223_001208"
     CDS             complement(8813..10129)
                     /locus_tag="IV223_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8711507.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(10279..10977)
                     /locus_tag="IV223_001209"
     CDS             complement(10279..10977)
                     /locus_tag="IV223_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8711508.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(11266..12255)
                     /locus_tag="IV223_001210"
     CDS             complement(11266..12255)
                     /locus_tag="IV223_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8711509.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(12335..13168)
                     /gene="msrA"
                     /locus_tag="IV223_001211"
     CDS             complement(12335..13168)
                     /gene="msrA"
                     /locus_tag="IV223_001211"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8711510.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(13343..>13709)
                     /locus_tag="IV223_001212"
     CDS             complement(13343..>13709)
                     /locus_tag="IV223_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711511.1"
                     /translation="YSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEK
                     SQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISY
                     LVTLTAYLCLFFKIVFHKR"
BASE COUNT         4218 a         2803 c         2350 g         4338 t
ORIGIN      
        1 tttttctttt tcactctccc atttttctcc ctccatccat aaatcgtgga agtatccaaa
       61 acaacgtagc aagttaaaat aaggcttagt ccgtcatcag cttttcaaag aagcactgtc
      121 tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc atacacctcc tcaaatcttt
      181 tcttcttacc taccctattt ttatttacag gaaaatcaga cacttcttct tttttgtcaa
      241 aatttccttt ttcttctcgt cctatcattt ggcattctaa taaaaacgaa ctatccttgg
      301 atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca
      361 tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaagaca ggactattcg
      421 aagatgacag tcatttatat gatgaattcg aatcaatcta gcatcctatt tagttgaatc
      481 actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta aagcaataat
      541 gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag aaggcaaatg
      601 gaaaggggat cagatcacct tttattcatt ggatgatcac ggttcaaatg atggtgcacc
      661 gaccgacact gtagtggatg aatattggaa taaaaaccat ccattagaaa accaaaaagg
      721 ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt tgatggatct
      781 gttaagtcga ataccttctg ttactgagcc agggatgact gctgaagaag atacgagaga
      841 ttttgatgca aaacaccgaa cctttgacaa agcacgtttg atggaaggtg gcaaaggaat
      901 cattgatggc ggaaaactgg gattcaataa taaagaccgt attcttctga ctaaactgat
      961 tgctatgcct gattcagaag aagaaaaact agacaatatc actatcgaag aatatttcaa
     1021 agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa catttgcttt
     1081 cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga tttatgaatt
     1141 cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt tacaatcaat acgaaagtat
     1201 tatgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt tgaaccgccg
     1261 tgtcactgac tgggaattca aagaaacacc gatgcaagat gaaatcactg tgactggttt
     1321 gaaaattacc aatacactga ctcaagaaga agaatacgtt gtggtcgatg aagatacagc
     1381 agttattttc actaatgggt ccattacaga ttcagctact ttaggagatt acgaaacacc
     1441 agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag ccagtgaacg
     1501 tttctacaac ttgggaaatc ctgacaaatt ctttgctgat cgagatgcaa gcgaatgggt
     1561 cagctttacc ctgacgacga aaaaccattt gttgttgaat gaaatcaccc gtatcacgac
     1621 tcaagaacca ggaaatgcct tgaattcatt tatttcaacc acaccgatca cgccgttagg
     1681 ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa ccgcatttta cgtctcagaa
     1741 accaaatgaa acagttattt ggggttattt cctttatcca agacgacgtg gcgaatttgt
     1801 ggataaacag tatattaaaa tgaatggaaa agaaatgcta gaagaactga ttggacaact
     1861 ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa gaaacagaaa ttttcgacag
     1921 cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcgttgttca ataatcgtgc
     1981 caaaagcgat cgaccaaaag tcattcctaa acattcgacc aatctggcct ttacaggaga
     2041 atttgtcgag cagccttatc agatgatctt tactgaacaa agtgctgtac gttctggaga
     2101 aatcgctgct ttccattttg cgggtattcc tatgtcccgt cttgtcaaaa caccgcgtta
     2161 cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa atgtttgaat aatcaagagg
     2221 ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc aggctaaaac agcttcttat
     2281 gtttttgatt tggttggact tattaccgca gatgcaagct tttgaacacc gtttaccaag
     2341 aggttatgaa aaagctgccc tgcatcaagt aataagagca gccaagctaa aacagcttct
     2401 tatgtttttg acttggttag acttattacc gcagatgcaa gcttttgaac accgtttacc
     2461 aagaggttat gaaaaaggcg tttagtttcg agtatgaaga aaaattttgt gaaaatggtt
     2521 cctcacgttt ttgcataaat ttgacttaat accgagaaac tgatcccttc tgtcataacc
     2581 tctttttatt tatgtctgtt tctttttgaa aacaactatt ttttcaacgt ttactctctt
     2641 gccaatccaa catcatctaa cggatacaac tatttcaatg attaccatta cactaaaaaa
     2701 tcggaaacac acctctccga acacaaatac ataagactat agaatgtttc atttaagaaa
     2761 aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa atgaaattat ctgtagtact
     2821 aataatgcag acaattttat gaatctgaaa ggagcatctc caatgaaagc agtcacttgg
     2881 caaggaaaac agaaaatgga aatcagaaac gtagatgatc caaaaatatt agaaccaaca
     2941 gatgcaatca tccgtatcac agcaacagcc atttgcggct ccgatctgca tctttaccat
     3001 catggtgaag cagttttgga aaaagggtat gttgtaggac acgaaccaat gggaatcgtt
     3061 gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc aacgtgtggt tatccctttt
     3121 aatattagtt gtggtcattg tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa
     3181 tcaaatccag atcagttata tggcgggctt ttcggatttg gcaagttgaa cggtaatcat
     3241 tggggtggtc aagcggaata tctaagagtt ccttatgcag actctacttc atttgttgta
     3301 ccagataatg atcttcctga tgaaaaagtt ttatttctat cagatatttt gcccacagct
     3361 tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata cagttgtgat tctaggttct
     3421 ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc
     3481 gcagtagatc cagtacagca ccgtctagat aaagcggcta attacaatgg ggttgaaact
     3541 tatttcttag aagacacggc acaagctgga acagaattgt atgaactggc taaaggtggc
     3601 gcagatgtca ttatcgattg tgtaggaatg gacggattag agcctgtcaa agaaaaagcg
     3661 aaaaatttag taagcttaca aagcggcaca atctctccat tacagatggc atcgcaagct
     3721 gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata tgactccagc ttcttcttac
     3781 ccgctgcaag aattttttat gcgggatatc gctgtcaagc atggacaggc accaattatc
     3841 catctaatgc caaaaattta cgcaatgatt gcagaaggac tattcgatcc tacacaaatc
     3901 attactcatt cgctgccact atcagaagca gcacaagcat atgagatctt tgacaaaaaa
     3961 gaagataaaa atataaaagt tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt
     4021 ttctttatgc ccaaaatcaa acgaaatacc catgagatcg aaataggaaa agaagaagat
     4081 atcgaaatac gaggacgtcg cttccgcttc tatttccaaa atcgttacac attgatctca
     4141 ctggctgtcg atttattaac cgggattttc tatatttttg gaagccttgc ttccttaact
     4201 aaaatccccg atcgatatgg cacctatttt tatttggctg gtgctatttt cttgacgatt
     4261 cgtcctattc tcagaatcgt aaggaacatt tttatttacg acgaaaaaaa agcaatgaag
     4321 agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat ctcatgcctc ttttttcacc
     4381 ctatcgtatc actttgatac agacgagcgg tcgtcccact ttcttccttg accattgtca
     4441 aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc tgtaattaag taaagttttt
     4501 cgtacccaag ttgatgcatt tcttttctga tttccgctaa tatccgcttg gctatcccct
     4561 gcttacgctt ttccggttca acaaacaggg cacaaagatt tggtgtcaga tcagggcggt
     4621 tatggaagtc gttttcaatg acgcccgctc ctccgacgat ggtttctttt tcatctaaga
     4681 ccacatacca ttgaggaata ctttccttat tagcgattcc agtacgaata ctttcctcat
     4741 acacaacagc aggaactccc cattttgatg aaaaccaatt cgcgctttgt tttactaatt
     4801 ctgggtgttc tcttaatttg atccagttat atgtaaccaa tgtttaaacc tccgactaaa
     4861 ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat tagcaatgac aacaatagtg
     4921 atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt tttttgatta atcaagcatc
     4981 taaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggta gatagatcaa
     5041 aatagaaaga accaaattga cagcggctag tacaccacct gcaatacttt ttgttgccaa
     5101 tacaccgcca ataattggtg gagttaccca tggaggcata acggtagctg ctggcactaa
     5161 acctgtagaa gtcgcaaagt aagcaacact aactaagacc attggtgtca aaataaatgg
     5221 aatgaacatg atcgggttca acacgatagg taacccaaac atcgttggtt cattaatatt
     5281 gaagacacct ggcgcaatcg acaagtttgt tacaaccata tatggtttgt ttttacgtcc
     5341 tactaaataa atagctaaca gtaaacctaa agttgcacct gttcctccta agttgacaaa
     5401 gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta tgtccagctg cgattgcatt
     5461 aacgtttgct tcaatggctg gcgcattgat tgtttgcatc aaaggatcga ccatattcgc
     5521 tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat gcaagcagta aggcagatgg
     5581 ataagagttt gcaagtccca taaatggttc ttggaccact gtatagaaag aacctacgat
     5641 atcatcaaca ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag agatagtgat
     5701 cattgctggt agtaatgaag caaatgcttt tgcaactgcc ggtggcacac catcaggcat
     5761 tttgatgatc aatttttcat ttccgcttaa acgtacaaag atttctgttg aaataatccc
     5821 aacaataata gcaataaaca aaccgtttga gctcatacct aatgctccgc ctaaagcgaa
     5881 gaaagagcct aaagaaatca cacctgcgga aacaccgtct tttcggtagc cgttagctaa
     5941 atgataagca agcaagaaag caatcaagac agaaagaatg gcaaatgtac cgttccaagc
     6001 agcagccccg aatcctttcc atgattcacc accaaaaatt gagttcatca attcttggaa
     6061 cccgggaatc ggtaagttat tgatcattac tgctaatgct cctaagatca ttaacggcat
     6121 actcaccata aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa tttttgatgc
     6181 tacaggtata aaatgtttct ccataaatgc aacaaattta tccataaatc ttccctcttt
     6241 tctttactgt agattacgcg ttcaatcgac gatacagatc aatgatctct tttgctaaat
     6301 ctgtaaaagc tatagatgtc atcagatgat cttgactgtg gaccgtcaaa agcgtgactt
     6361 caacgtgatt tccttgtgct tcttgtgtca gcatctctgt ttgagagtga tgtgcttgga
     6421 ctaaagattc ctctgcatct ttgatttttt catctgctaa agtgaaatca ccttttttcg
     6481 cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg atcagaccca
     6541 tgatggcttc tagatttttt tgatcttcca tttattgtat cctccgataa tttttattat
     6601 ttgtttatta gtttttctgc ttgttcgagt actttttcac cattcatcat gccatagtca
     6661 gccatattga ttacatctaa aggaatgcct ttaggtgcta gcttttcagc aaattgattt
     6721 tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta catttttaga ttctagttga
     6781 ttatccgcat ctgaagcaga tacagcaaag atgtctgctt ccattccttt tgcttctgct
     6841 gccttttgca tttttgtgac taggagactt gtactcattc cagctgaaca tacaagcatg
     6901 attgttttct tttccatttt aaacacgttc ctttctatac ttacacttgt atataagcaa
     6961 gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat catcacattt aaggtctata
     7021 ccaagttgca tacacactaa aaaagaaagc gcacactaaa cacacaaatt aatttttatt
     7081 accttttttc ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc acaacttttt
     7141 tcaaataaac ggtattcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac
     7201 ataagatgtt gtttttactt tgctattctt attacttgat gcaggacagc tttttcacaa
     7261 cctctcaata aacggtgttc aatcagctga ccctgggtat tagctcaaaa cgctggattc
     7321 acaacctctt ttatttcaaa taaccagtaa atacaatgaa catctacaaa aaacttctat
     7381 ttatttatag atttcgtcag gcaaatccaa ttctctctta tcaatcaaag tctgatacca
     7441 cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc aattcgattc gaactaatcc
     7501 atatcgattc ttaaatgcat tcattggtga cacacagtca gtgaatgccc ataacatata
     7561 gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag taatgcaaat gctgtccgat
     7621 aaattcgata cggtaatcat cttgcacgat tcctttctca tccatatact gttcttcata
     7681 ttctcttccc ataccgtttt ctgttatcag ccaagggata ttatgataat tttcttttag
     7741 atacatggct atatcataca tgattttcgg atagatttcc catcccctgg aattattcat
     7801 tttcttccca ggtaaatcaa agacttcata atatcgttca ggatggaaag gtgtctcttc
     7861 attccattga taacgagggg cgcgaacgcg tttaggataa tattgattga ttccaaggaa
     7921 atctactgta tgatgctgaa tcgtttctaa atcctctgtt gcaggatcga aataaatcga
     7981 ttgtttttga cacaactcca ggaactcttc tgtgtagtat ccttttacca tcggatcaaa
     8041 aaatactcga ttataaaaca aatcatacat ataagcagct ttttggtctt ctggagaatt
     8101 ggatcgagca tagaccattt caggatttaa aatacagcca atccttccac cataatttcc
     8161 gctgcggtaa atctctactg ctttagccgt tgctaaaact ttatgataat tccacatcat
     8221 ccactttttt gtgttttgtt catgcggcca gcgaacagca tccaaatagc aacgcgtttg
     8281 gggaacgatt ggttcgttga atgtaaacca gttttttacc ctatcaccgt aacgatcaaa
     8341 ggcaatttga gcatacttag cataaaaatc aactactttt cttgaagacc agccatcata
     8401 tttttccaaa agaaaagctg ggacttcata atgttccaaa caaatcatcg gttctacgtc
     8461 tgcttcgatc aaagagtcga tcatttcact tatatgctga gcatattctt catctactat
     8521 acagttctca taatcagtaa aaaaacgagc ccaattgata gaagtccgat aatgtgtcag
     8581 atggatggat ttcatcagat ccacatcctg ctggtattgt tccataaaat tagttgctac
     8641 ggctgggcca tacccattat gccaaacaaa atgttcattt ttataccagc tatctataaa
     8701 cgaatcctgt ccttcttttt ttcctttcca accttccgtt tgccaagcag aagatgcagc
     8761 accaagaata aaattttttg gaatagtaat tttcatgatt tcctcctgtt ttttagatcc
     8821 catcattttc cacacgattt gctactttta caaatggcag atagatcaaa atagaaacaa
     8881 taatacaaac aagttgcgta ataactgcac cgatatttcc tgctgttgcc agataagcat
     8941 tgacgatcgg cggcattggc caagggacca ttacaactgc tttcgccgca aatcctaaag
     9001 cggttgcaaa ataaccaatc gttccagtga taagaggcgt taagataaac ggaatagcta
     9061 aaattggatt cagcatgatc ggcataccaa aaataactgg ttcattgatg ttgaagatcc
     9121 caggagcaag ggataacttg gtgatttgtt tcatatcttc ccgttttcca actaacaata
     9181 ctgcaatcag taaaccgatc gtgactccag acccgcccat actcatatac atatcccaaa
     9241 atggcatagt aataatgttc ggtacttctt ttccagcttc aaaagcttcc gtatttacag
     9301 caattgccgc taaaagcaac ggttcccgta tcggcttcac catttgattt ccatggatac
     9361 caatcaccca aaagacttgc gcaataaaca tcaatagcaa aatacccggt aacccttgta
     9421 cgacaccttc taatgggcgt tgtacgacat tgtaaatgat atcataaagg tacattcctg
     9481 taagttcttt gatgataaat cccgcagccg cgatacccga tacagtcaga atcgtcggga
     9541 ataaagcaga aaaactagca gaaacattcg ttggcacggt atcaggcatt ctcagctgta
     9601 gtttcttctg tttcctcaac cagcaataaa gttcaataga aaggatggag accaacattc
     9661 ccaaaaataa ccctttcgta tctgtatatt gtttagccaa tacgttttct actataacag
     9721 tctgattttc attcactaat tcataaacag ttggattaac agataaataa gacatcactg
     9781 cgatgatacc agggaataca ccatccactt tattcagctt ggctaattcg atccctatca
     9841 gaaaaacagc atagatggtc aagaaactta gtgtgacata gctgatggca gatgcgatag
     9901 gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc taagccattt tccgagtcaa
     9961 agaccattgc tgaaaacaat gtgccaaaag cacccgtaat aattaccgga atcaatgcag
    10021 caaatgcgtt ttttatcacc atgatatacc ttaaattatt gattctcgtt gccacgttcc
    10081 ctaaaacatt ggctaattta tccataaaac tcattccaga tcccctcatt cctgcttctt
    10141 ctccttgacg tcatctcttt ttgtctatta gtcagcgctt acatttcgat gaacagaaac
    10201 tatttactcc tcttttttac caaatataaa acaaaaattc atctgatgta tttatcataa
    10261 aatacatcag atgaactgtc aattagtttc tttttctcct attattttct gtaattctct
    10321 ccgatttatt gccatatgga cagtcattgc atcaaacgca gcaagtgcat tccgcctttc
    10381 gatggcctga acgatctctg tatgaatatc gatcgtctcg attttcagat gctggatatc
    10441 ataattttta ttaaacaaag caattgattc attaatgatt gggataatat gcagcaaggc
    10501 tacgtttccg ctagcttctg caatcatcgt atgaaaatcg atgtctaatt gaacatgttc
    10561 ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt aattttttca tttggacgat
    10621 ttgattcttc gttgcatatt ccgctgccaa acctgccatc ttaggctcca ataaatatct
    10681 cagttcaaat aagtctcgta tcatccgatc ctgatcttca atcaaagaaa aaccgagtgg
    10741 gtcattggca atccctcgct tatcactgac aaatgtcccc gctccttgcc gaacttcaag
    10801 gatattcctg gaagctagag cccgcacagc ttctcgcacc gtgcttcgcc ccacctctaa
    10861 ataatcggac aattcatatt cattcggcag tttatctcct acttgcaact gcatttcctt
    10921 taaatatcta aggatcttgt cagtagttac ttctactagg gttttatttt cttccaaacc
    10981 acatacctcc ttttcatctg atgtattttt tcgtaattat tataacacgt ttgaatcatc
    11041 tgatgatttt ttgtatacaa aattgattaa tccgttcata gacatacaaa cgaagaagat
    11101 atttaccata aaaaagacta ggataaaatt cactcctagc cttcgatcat ttttattttg
    11161 aacgacaaaa aatatgatgt tcaataacag acgatcttca ttcgattctt gatgtcatct
    11221 tttgtttttt ttaatatatg gataaaaagt ttgattacat cgtttctatt ctagaatttg
    11281 gtcgattctc aattcttctt ctggtgagaa atctaaatta tccatcattc gaacactttc
    11341 ttgtaactga cttaaccgac ttgctccgac cagtacactt gtaacagatt tttgtcgcaa
    11401 attccatgca agggccattt gcgctaatga ttgtcctcgt gatacggcga ttgtttgcag
    11461 tgctttgatt ttttccagag ttgatccaac ttgttcttct gaaaggaatg gtatttcctt
    11521 acgatgtgct ctagaatctt caggaatacc atgtagataa cgatttgtca gcaacccttg
    11581 agcaagcgga ctgaacgtga ttgcccctag cccttcttct tctaatactg gagatagatc
    11641 gtcttctagt agtgcacgag aaaacatatt gtagcgcatc tgatgaataa tgaacggtgc
    11701 ttcctttctt tttaaaattt cagtcatttt ttttgtatct tctccattat agttggatat
    11761 accaatatat aatgcttttc cttgtcttac aagttgcatc aaagcttctg ctgtttcttc
    11821 caaaggtgta tcagggtctg ggcgatgatg atagaagatg tcaacataat ctagccccat
    11881 tctttggaga ctttggtcac aacttgaaat gatggattta cgagagcccc attccccata
    11941 tggcccttcc cacatatgat aacctgcttt gctagaaata atcagttcat cacggtaggc
    12001 ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct gcagctccag cagggcctcc
    12061 ataattattc gcaaggtcga aatgggtaat tcccatgtcg aatgcgcctc ttaaaatctc
    12121 tctttgatta tggataggat cataatcacc aaaattttgc cataatccga gagataaaat
    12181 cggtaatttc aaacctgaat gaccggattt acgatattcc attttttcat aacgtgcact
    12241 atctgcaata tacaattttt ctcctcctca tcaaatcttt ttaaaacgct ttcttttttc
    12301 tatttttagt ttactacatt ttaaagaaaa ggagttaatc aaaatgattt tttcgtatat
    12361 ttttttgcca tttgtttttt actcgttgat agatcagaaa ttgattacgt gcttgctgga
    12421 tttttttata acgtttcggt tgttttttgt aaaattgctg atgatagttt tctgctggcc
    12481 aaaacgtacc ggctggttcg atttttgtga cgattggttg gtggaaagtt cctgaactgt
    12541 ctagtttctg ttttgattgt tccgctaatt ctttttgtcg ttcgacttga taaaaaatca
    12601 ctggacgata ttgcttccct ctatcttgaa attgtccaaa tgtatctgtg ggatcagtaa
    12661 tttgccaata gattgtcagc aactcgctat aacttatttc tcttgtgtca taaatgatct
    12721 ccacagcttc cacatgacct gtgtaaccac cacttacttg atcataattt ggtttttcaa
    12781 catgtccgcc cgtgtatcct gacaaaacag ataggatccc ttttttcgat tcaaacggct
    12841 cgaccataca ccagaaacat ccccctgcaa atactgcacg ttcttgatag tcagcatctt
    12901 gcatggcatg cttggaaaag tcatattttt gctccttatc aaatccttcc gtaatcaata
    12961 aataaaagtc cattacatca ggcgtcagat tcattcggat agctaatgga cgtagtgttg
    13021 cttctagttt tgataactgt tctttaacgg atatattttc tcttatgtca ttctttgtct
    13081 gaatcaatac ttttcgttcc caatctcgtg tattcggatt caaaatcaga ttataaagtg
    13141 tttgaatgaa tatttgttcc tcatccatag attctcctcc tttccgaaga agacaagcta
    13201 gtgactttag ctgtaattta tcataaagtg aggaaataag cgattacttc atccccctac
    13261 agatttttcg tataaaaaag agagaatcct tatcgacaga ttctcctttt taatcaattt
    13321 attttataaa tgaaaagtta atttaccttt tatggaaaac tattttaaaa aataaacata
    13381 aatatgcagt taatgtaaca agataagaaa taatagtcgt tactgtataa cttgtgctta
    13441 ctttaatatc agcaactcct ttgactccgc ttatttgtac caatccatta gcattcacct
    13501 gctcctttac aggttgatga tttatttcta cattcgttcc taaataagca tatacaggaa
    13561 gtgtgattac ttggctcttc tcattagtta ctctatatat gatagaacta ccagttgttt
    13621 tataaggaag gtttacccat tgactgccaa tctttgcttc gtgttttatt atatgctcct
    13681 tattctccaa tgaagcgata ggagaataa
//
LOCUS       DACDHJ010000052        13507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDHJ010000052 DACDHJ010000000
VERSION     DACDHJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.053"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(95..1351)
                     /locus_tag="IV223_001213"
     CDS             complement(95..1351)
                     /locus_tag="IV223_001213"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8711512.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            1931..3232
                     /locus_tag="IV223_001214"
     CDS             1931..3232
                     /locus_tag="IV223_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8711513.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3301..3900)
                     /locus_tag="IV223_001215"
     CDS             complement(3301..3900)
                     /locus_tag="IV223_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8711514.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4154..5035
                     /locus_tag="IV223_001216"
     CDS             4154..5035
                     /locus_tag="IV223_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711515.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5048..6658
                     /locus_tag="IV223_001217"
     CDS             5048..6658
                     /locus_tag="IV223_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8711516.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6735..7394)
                     /locus_tag="IV223_001218"
     CDS             complement(6735..7394)
                     /locus_tag="IV223_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8711517.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7677..8342)
                     /locus_tag="IV223_001219"
     CDS             complement(7677..8342)
                     /locus_tag="IV223_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711518.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(8489..9094)
                     /gene="dhaL"
                     /locus_tag="IV223_001220"
     CDS             complement(8489..9094)
                     /gene="dhaL"
                     /locus_tag="IV223_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8711519.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(9122..10105)
                     /gene="dhaK"
                     /locus_tag="IV223_001221"
     CDS             complement(9122..10105)
                     /gene="dhaK"
                     /locus_tag="IV223_001221"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8711520.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(10107..10502)
                     /gene="dhaM"
                     /locus_tag="IV223_001222"
     CDS             complement(10107..10502)
                     /gene="dhaM"
                     /locus_tag="IV223_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8711521.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            10656..10976
                     /locus_tag="IV223_001223"
     CDS             10656..10976
                     /locus_tag="IV223_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8711522.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(11088..11957)
                     /locus_tag="IV223_001224"
     CDS             complement(11088..11957)
                     /locus_tag="IV223_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8711523.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(12053..13357)
                     /locus_tag="IV223_001225"
     CDS             complement(12053..13357)
                     /locus_tag="IV223_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8711524.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         3753 a         2778 c         2476 g         4500 t
ORIGIN      
        1 attaacgaca accagcgttc tattttagga tgggtttcaa aattggaatg ttgtaatagt
       61 atttcccttt tttaaggtaa aaacttacta aattctaatc aattactttg gccaaaaagt
      121 aatttttctt accttttcga ataaccacaa acttaccatc gaattcatcg gatggattta
      181 tgacaaaatt taaatcggta acgcggtcac cgttaatact tatagctccg tttgaaacat
      241 cttcacgtgc ttgacgttta gatggttcaa tttttgtgga aacaagcagt tccacgatat
      301 tttcaggtgt gcttgaaatt tcaacgttcg gcattttacc gaatccttgc gcgatttctt
      361 ccgcatttag ttctttaata tttccagaga acaatgcttg cgttattttt tgcgcttctt
      421 tcaagtcttc ttcactatgc acaaatcttg taacttcttc tgccaaacgg cgttgtgctt
      481 cacgtttctc aggttctgtc tctacttttt tcgctaagtc ttcgatttct tcttgagaca
      541 agaaagtaaa gaatttcaag tatttcacta catcgcgatc atcttgattc aaccagaatt
      601 ggtagaactc aaatggtgaa gttttcttcg gatcaagcca aacggcaccg cctgcagttt
      661 ttccgaattt cgttccgtca gccttcagca tcaatgggat cgttaaaccg aacgcttctg
      721 cttctggtcc ttctaatttt cggattaggt ctaatccagc tgtgatattt ccccattggt
      781 ctgctccacc gatttgcaat tgcacatcgt atgttttatg cagatgcaaa aagtcgatag
      841 attgcaggat ttggtaagta aactctgtaa atgagattcc tacttccaaa cggctggcaa
      901 cgatatcttt tgctaacatc gtgttgatgt tgaagttttt accgtagtct ctcaaaaatt
      961 caagtaaaga gatttttgac aaccagtcgt agttgttcac aaatgtgata tttgcatctt
     1021 taccaaacaa ttttctcatt tgattggaaa gtgcgtccac gttatgttgc acttgttcca
     1081 tcgtttgcaa cacacgttct gttttgcggc cgcttgggtc gccgatcgtt cctgttccgc
     1141 cgccaatcag aatatatgga tgatggcctg ccaattggaa tcttttcatc atcataaatg
     1201 gaatcaaatg tccgatatgc atcgaatcac cagttggatc aacgccgcaa taaagtgaaa
     1261 tgctcttttc ttcaacgagc tcgcgaagtc tttcttcgtt tgtttgctga ttaatggcgt
     1321 cgcgccaagt taattcatca ataatattca ttttactgac ctccgtgtag tttttttatg
     1381 aaagcaggaa cctgctagca tatgtaatta attatttctt caggatagga taggaaataa
     1441 aaaaagtccc ttggcatata agccaaggga cgaaatctcg cggtaccacc caatttgtat
     1501 gtctagatat ccaacatacc tctcgaacat ttaacgctgt cgcgtggcaa tcactgccaa
     1561 agactcctga aatgtaattc gctttaaagc atgtactggt ttgcagcgac caccagcttt
     1621 ctgaaacatt ggctttaaca actacttcgt ttcgatcttc gtcttgttat tattaatatg
     1681 tgtctatcat agagcgcaac tttttctttg tcaacagaaa aaggatattt tttttaaatt
     1741 cctcattttt tcttttttga atccgctcta attccgaaca ttttattttc aattattgtt
     1801 aaaagaaata actttttctg tattttttct gcaatttatc gagaagtcac tttactttgc
     1861 ctcttattct ttcttcgtta caataaggaa tgggacaagt aaaaaaatga aaaagcaagg
     1921 gacgaggtat atggaaaaaa agcaaaaaat tattttagga gccacttctg ctttaactat
     1981 cgttatttta attctaagtt tgttttttgt catccgatca tctacaaaaa aagaaccaat
     2041 cattcctgaa aaaaaaataa cagaaaaagc aaaagaaccg acagatgata agcaacaact
     2101 agttactcaa gcaaaaaatc tggctcaaag ctacgattat gaccaagcga ttgcgttatt
     2161 ggaaaaagat agtaagaacg gatctactga aacacaacag cttcttcaaa cactaaaaaa
     2221 ggagaaagaa caactgatcg actggtctga tcctactcag atctctcatg tctttttcca
     2281 cagcttgatc gtggacccgc aaaaagcctt ccactctcaa caggcacaag gatacaaaga
     2341 ttatatggtg actgttgaag aattcaaccg ttcgatcgaa caactttatc aaaatgattt
     2401 tgttttagtt aatttgaatg atttgatcca aaaagacgaa caaggaaatc tttcctttaa
     2461 cgggcttgcg ctacctgaag gaaaaaaacc attggttctt tcacaagatg atgtcagcta
     2521 ttatgaatat atggataatt ccggttttcc tagccgattg gtcgtatcaa ataacaatga
     2581 aatcaaaaat gtctataaag agaaagataa agaagaaatc ggtgactttg acatggttcc
     2641 tctgatcgat gcctttgtca agaaacatcc tgatttttct tatcgaggcg caaaaggtac
     2701 cttggccctg acaggttaca acggagtttt aggttatcga acatcaaaat ctcaatatgg
     2761 agacaatgaa aaaacaaaaa aagagattaa agaagctaaa aaagtggcgg aacagttgaa
     2821 gaaagacggc tggagctttg cttcccatac atggggtcac ttgaatatga ctcaagcttc
     2881 actagaagat gtaaaaaaag acaatgaatt gtggcagcaa gaagttgcac ctatcctagg
     2941 aaaaacaaat attctgattt atccatttgg tgcagacatc agcgactggc agccttattc
     3001 agcagaaaat caaaaatttg cttacttgaa gagtcaaggt ttcgatattt tctgtaatgt
     3061 cgatgcttct acaccagctt ggggacaatt aggtacggat ttctatcgaa atgctcggat
     3121 caatatcgat ggcattcgtt ttcaatctga tttagacgga aaaaatccta ttcttgatcc
     3181 attcatcaat gtccatgatg tctatgacaa agaagcacgc gaatctgctt gattttacgt
     3241 catttatgaa ccaaaaaagc cgaactgaca ttttatcgtc agtttcggct tttttatgct
     3301 ttatttcttc ttcggtgcga ttcccttgat caggacttct gtccattctg ctgtatatgg
     3361 aagcagtttt gcgtatatct ccggcttaga aattccttct accattgctt gaaaatagag
     3421 gatcaagaac tcatccgaat atttcaaatc cacttttcct tctttccgcc ctcgatggaa
     3481 aagatcaagc atgatactga aactttcttg cgtatacgca tccatcgcgt cagaaaattc
     3541 ttttttttct ttttttgtat agtatgtcat caaatcgata taaaattgtt tgttcactcg
     3601 attgagataa cggattttgt tttcactcat ggcaatcagt gtttcttcaa atggaagatt
     3661 ttccgcaatc aacgctttcg cttcttgccc taaatcattc ataaatgcaa aaaatacttc
     3721 ataaatcagc ccttccttgc tgccaaaata tttaaaaatc gtcgctttac ttacctgagc
     3781 gttctcagct accttatcca ttgtcactcc agcggttcct atgtctgtgt tcatcaagtc
     3841 aaaagctgct tgtaaaatct ctttcttttt ctcttgtgct ctcttttcaa aaccgttcat
     3901 ttgtagagcc tcccgtaatt ttaaactttt ataatatgta tagttcaaat ttattctgag
     3961 gtttattata actatctatt gagaaagtat caagctagca attccttgtt tatgaattca
     4021 ttgacagaaa tttataaaaa gaatattatg tactaaaata ataaaaaaag ttttataaaa
     4081 aggtataaaa cctttttatt aaactattta atcttgttta gtatatattt ttagaaaaag
     4141 gagtggggtt tttatggcag aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg
     4201 tttcgaagct ttaaaagatg tctctttcac cgtacattca ggtgaagtcg ttggatttat
     4261 cggaccaaat ggttctggaa aatccacgac gattcggaca cttttaggga ttataaaaaa
     4321 agacggcgga acggcaacga tttttgggca agatgtatgg gaagatagtt tatctatcca
     4381 tgaacgtctt tcttatgttc caggcgatat ctctttgtgg ggaaatttga ctggaggagc
     4441 gatcatcgat ctcttcatga agcttcatgg aaaagggagt gctcaaaaaa aagatgaact
     4501 gattcaacgt ttctctcttg atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg
     4561 ccaaaaagtg ggcttgattg ctgctttggc tgttgattcg gatctctatt tatttgatga
     4621 gcctacttct ggtttagatc ctttgatgga aacgattttc caagaagaag ttgaaaaatt
     4681 gaaagaacag ggcaaatcca tcctactttc ttctcatatt ctcagtgaag tcgagcgact
     4741 agcggataaa gtcgtgatca tacgcgaagg aacactcgat gaagaaggaa cactcgatga
     4801 attgcgtcat ttgactcgtt cgaccatcac tatagaaaca gaagcagaga atagtcactt
     4861 agcacaattg aacggtgtcc atgactttgt gcaaaaaggt cacctcgctc aatttgctgc
     4921 tgataacgaa gctttcgatg tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt
     4981 tgaatctatt ccaccgactc ttgaggattt attcatgaga cattatgaag gatgaggagg
     5041 aagcgaaatg actgataaat tttcacgctg gtccaccctt ttttctcaaa accttagccg
     5101 tgactggaaa aagatcctga tatggctagt cggtgtcact gctttttctg gtggttttgt
     5161 ccctgcgttt gaagaattga cttccggaca aggattagct ggattatacg aaaccttgca
     5221 aaacccggca atgattgcaa tggtcggacc aactccagct tcgaatgctg ctgagtatac
     5281 tttaggagcg atgtatgcaa atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat
     5341 ctctgtttta cacgtggtga gccaaacgag aaaaaaagaa gaacttggtc tgattgaact
     5401 tattcgttcc tttcgagtcg gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat
     5461 tctgatcaat ttgcttttag caatatcgat cagctgtacg atgatttttt tccaagcgga
     5521 ttcgatcact gcagaaggtg cttggctttt tgggctatct gtaggtagta tggggatttt
     5581 ggggagtgcc gttgcactgt ttgctgcaca actgatgcca agttcagcca gttcgaatgg
     5641 tctgtcttta gcgtttttag gtggacttta ccttctacga gcaataacag atgtagctga
     5701 ttcctccctt tcgatgtgga atcctttagg ctggtcttat ttgacctatc catttacaac
     5761 taacaactgg attcctttgc tttatttgtt tttgttcagt ctaatcttga taatcagttc
     5821 atttgtttta gaggaacatc gagatttgaa caccggctat atccccgaac gtgaaggaaa
     5881 accgtatgct aaacgttctc ttttatcctt gcgtggttta ttattctatc taaataaagg
     5941 gatgacaatc agttggctta tcacttttgg tttgatgggg gcggcatacg gctctatcta
     6001 cgggaatatg caacagtttt tagaaagtaa cgacttaatg aaacagatgt ttatccaatc
     6061 tggcgtctca attgaaaaaa actttacaag cacgatcatg gtagtcttga tttgtctagc
     6121 tgctatttta ccaattgcta gcatcaatcg tttatttaca gaagaaaccc acggacgtat
     6181 gaatcagctg ttcgcaacaa aaatttctag gaaccaactt tattggacca atatgttact
     6241 agctattcta tttgctatat tggcaatcct gattagttct ggtgcactag ggctttctgc
     6301 tttggcttcg atgaaggatg cgaaagacat ggatctattg atgtttctta aagccgggtt
     6361 caatttcctg cctgccgttt tagtgtttat cggaattagt ggctttctat tcggatggct
     6421 tcctcgaatc ggtaaacttg cttatatcta cttagcttac tcctttgcac tcaattactt
     6481 tggcggaatt ttggatttac cagaatggtt ttccaaaaca gctgtcccta gctgggttcc
     6541 acggatgcca aacgattctt ttgatctttc cgtgttcctt acgttatcat gtatcagcct
     6601 tgcactgttc gtccttggtt ctattggtta taggcgaaga gacttactag aaggataata
     6661 aaaaagcatg tacaatttca tgctagcctc caaatctggg tcaaaaatcc aacatttgga
     6721 ggtcattttt ttatttacaa gtcttctaac tgctctttat caatgatcca gacttctcga
     6781 ttctttcttt ttacataacc attctcctct aataatttaa attttctaga taaagtttcg
     6841 ggggtcgtac cgataaatgc agcaagttct ttcattttca ttggcaggaa aaagtgatcc
     6901 ttccctgtcg ctttagctaa gtcaagcaga tagacagcga ctcgttcttc tactttttcc
     6961 atcatcaaaa aagttgtctg ctgctgtact tttgttaatt tctcaacgtt gattgctaaa
     7021 agtttcagac taagggcggg ataatgcagt aagagttccg agaaatcttt ttgtctcaat
     7081 atgcaaattt ccgttttttc tagtgcttcg ccaaataaag actcatttgg aacgcctagc
     7141 aactggctct ctccctcata tccgcccggc tctattactc gtaaaagctg ttcctttccg
     7201 ctagcagata attgatacac tttcatactg ccggaggcca cgatcactaa ttgaggttct
     7261 ccattcggcg caagaatctg ttctcctttt tccacatagt gatgtttgac aagtgcgtgt
     7321 actttttctt gatcttgctg tactaagtga ttgaacaaag gaacaaatga aacacataaa
     7381 tgatcatgac tcataatctc tcctccttct aaacagctat ttctaataaa gaaacataac
     7441 ttcccttatt ccaactgata attttctttt atcatagcat aaacgtcaag cgaaccagat
     7501 accagcaaat agagtggatt tctttttgtc ccttgcttta ttttccttct tttgagacct
     7561 taagtaattg attcctatca attttttaaa tatttacttt ttgaataatc aaaaacaaac
     7621 cttcactcgc aagccgagag taaggtttgt ttttcgcatt gcttatttcg tgttgtctat
     7681 tgaaattttt ccgcacctag cttttgatat agtggtgcta gttcttgtaa ggtttcagcg
     7741 gttatttgct caacgtgttc gatttcttgc ggatagaaga atagtttacc catttgaaag
     7801 gttcggtctc tcatcgcttt tttcaactct tcttgtttct ttgtatcgga caaatagtca
     7861 gacaaagcta agtggtcgtc aagttcatgt tctggttgcg gccatatgta ataatccttg
     7921 atatcgactg ttttgctcca ctctttcagt atcgcaagca actggttata cgctgcaatg
     7981 ctaccatatc gttcttcgct tttataatgt tggtacatca aatagatttg gtattgtttt
     8041 tgattcaaca aaactgctag acaggcattc tcgtcttgac gagattcgct tcggtacgcg
     8101 ctccaaaaat gactgcgtaa attccagcca ttcgtccagc tttcgatttt aggtttgctc
     8161 atgccgtaca tatcaggcaa aagtgcagct gtctggagct gaatctcttt ccatttttgc
     8221 cagtgttctt tatattcttt cttgatttgt tcaatcttat ctgttggatg gctctctttg
     8281 agctgtttga atgcgtagat tggtcgatca aacaactgat aatctgattt tccaagttcc
     8341 attgtttcgc ctctttccgt tatcttttgt attctacctg aattttgcga tatggacaaa
     8401 tagaggttgt gaaaaagctg ttctgcatca agtcataaga acagccaaac aaaaaactgc
     8461 gatgagcatc accgcagttt tattgaactt aaggttgatt gatcacatct aaaagcgttg
     8521 aaaataaaat ggcactagaa gcagctcctg ggtcgatatg tccgattgca cgctctccta
     8581 gataggaagc tcgtcctttt ttagctacca agactttggt gtgttctttt gcctggtcaa
     8641 tgatttcttt tgttaaattc cctgccttca atgcctctgt gactggcagc caaacatcta
     8701 acatcgtttt atcgtcagct tctgcctttc ctcggctttg aatacccgct gttccctgct
     8761 cgatcacttc gcctaatttt tcttgagaat caatagaatc gacctctttt gtcgctttca
     8821 tcatgttcat gaatgcagat ccgtataacg gaccagaagc cccaccgact ttcgatatca
     8881 tcgccataga gaatactttg aatgtatcgc tgattgtttc aggatgatcg gcttgaaaag
     8941 cttcttcata agctgaaacc cctcttccca tgttatttcc atggtctcca tcgccaatcg
     9001 gggtatctag atcactgaga tattgtttgt tctgattgat tgcctctgca aattgcgtga
     9061 gccattcttg gatttcttga acggttagtt tcatattatc acccttttat cttgagattt
     9121 tttaccatga aatcgtctgt acattgcttt ctaaagaatc tttccattgt gtctccgtca
     9181 agtcgatcag tgttagagaa agaccttgca tatctagtga ggtcataaaa ttaccgactt
     9241 ttcggaaaac tacattgacg cctctttctt ccaacagagc taaaacatca ttcatgaaca
     9301 cgaactgttc catcaaaggc gtcccgccca ttccgttgac tagtacacct gcttcttttg
     9361 gtaaattcga gtaatcatct aaaatcttag taacaagttc ctttgccagt actttagaag
     9421 gctgcatctt ttctcgacga tagcctggct caccgtggat accaacacca tactcgatct
     9481 catcttctcc tagttcgaat cctggtttcc ctacttctgg tacagttgct gctcgaagcg
     9541 ctagaccgat cgtcttagtt gcctgtacga ccttttctcc cagttctttc aactcagcta
     9601 gggaagcacc gtttcttgct gcatcgccca cgattttgtg tacaaggaca gtacctgcaa
     9661 ctccgcgttt tccagcagta tacgtactat tttcaacggc gatatcatca tctacgatca
     9721 cactttctac ttcaatatcg tccatagcag ctaattcctt tgccatatcg aagttcaatg
     9781 catcccctgt ataattctta acgatcagca aaactccttt tccactatcg gcttccttaa
     9841 tagccgtctg aatctgatct ggagttggag aagtaaacac atcaccaagc accgctgcac
     9901 ttaacatccc ttcccctaca aacccggcat gggatggttc atgtccgctt cctccaccag
     9961 aaaccaaacc aacttgttcc gctttctgat tcttggctac aacacgggaa gattctacac
    10021 gatgaacaag ttctggatag ctttttacta atccttccag catttcttcg acaatattgc
    10081 ttggatcatt catgattttt ttcaatttat tcccatcctt tctttccggc attttgtgct
    10141 tctgttaaga tttgctgagt atcatctgtt ataccggcag tgatggctgc tgcaaaagct
    10201 ccttccacta aaggcgcatc caccagatga taacgttgct gctgctcttc ttccaacatg
    10261 tcttttgcca gttccgagtt caataccgcg ctaccgatat ccgcaaaaac aaaataacta
    10321 gctgcatcgc ttgattcatt gactgcttct acgattttca tcgggtctga tccgatctcg
    10381 ccatttattc cgccgagaga aaaaatttgc acctcttcgg atttttgcat ctgctcaatc
    10441 atttctttaa taccatctgt tactttttga ctatgagaaa taagcaaaat actttttttc
    10501 acaagaatct tcctttcttt tacatagttg ctaacgattg gtttcgcttt cattaatgaa
    10561 caattcaagt atataataaa tttctaattt tgggaaaata tgtgtagtgc ttcttgaagt
    10621 ttgttatact ttaattaaaa gagaggagtg atcgaatgat tacaacaaca acgaacagtg
    10681 tagagggaaa agccgtagta gcttataaag ggatcgtttt tggtgaggtc attactggga
    10741 tccatgcatt taaagacttg gaggcggggt tgcgaaatat ctttggcggc cgctcaaaaa
    10801 gctatgaaaa cgaactaatg ggtgcaagag aagaagcact tgccgaaatg gccgaacgtg
    10861 caaaagcatt aggagctgat gcgatcattg gcgtaaaaat ggactatgaa gtattaggca
    10921 gcgacaatgg catgctgatg gtcacatgta gtggaacagc tgtctccacg agataagtag
    10981 gggacatctg ttttaataac taaagagaga tgggacatgt agtggcactc aagaattaga
    11041 tttttcagtc taacttcgag gtactctacc ttcccatcct ctctttttta tttctttttg
    11101 cggatgattc cgtaagctgc tgcgactaac acacccgccc ctgctaagga ataactgaca
    11161 gaagctgttt tttcatttgt ttttggcaaa gtctgacttg ttgatttgga tggctctgtc
    11221 gtttgctgaa ctgtttctgt cgtgcttcca gaggtcactg atttttctcc tgccgaatgc
    11281 aaaatatctt ttattcgttg gaaactagct gctaagtcag ctttttttgc ttcaaaatca
    11341 ttagcaactg tttgtgcttc gctgatcttt ccgtggattg cttgactggc cgctgtgata
    11401 tccgcttgag tttgacttag ctttccagaa gctgtttgag caacctgatt taattgctga
    11461 ttgaattctt ttgcttgttg gatcttcccg tcaagatctg tttggacagc ttccgctttt
    11521 gctttcgctt cggcaagctt tttctcaagt tgtgcacggt catttcccac tttattttta
    11581 agatcagcta aatcttgatc gattgctgct tggtcttctt tcaatttgtt ttttgcagct
    11641 tctaatgctt cttcgagttc ttttctttta ttttccaagt cttgcaaatg atcgatttgg
    11701 tcagaagagg tagaactatt tattgatgta tcttctgaag atgaaatcgt ttctcctgaa
    11761 ttatctgtcg gtacattgga agtcatttct gtttccgagg caccgttttc agcaccagaa
    11821 gagcttatgg ttgttgaagg ttctgtttga gcaggttcag aagtttccgc aaaagctgtc
    11881 tgtcccaata agaaaatact ggaaacagca attggccaga ttaacggctt cattcttttt
    11941 ttcatcactt ttcccatcct tactactttt taactaattt cttattataa gcaaaaatgt
    12001 ttggaagaac cataaacaca acaggctgtt tgtctaaaaa acggtgcatc ttttattttt
    12061 ttgtacgttc cgggcttttc catgtcccgc ttccataacc taaaatcgtt ttgactgctg
    12121 gaatgaaagt cgtcacgatc gttatcgcgt tcactatcca gaacaaaagc atatatagtg
    12181 gtgcgaaaag aaggtatttc aattttcttc gtcgatcatc tacaatcagc gaagctagca
    12241 actggaaaaa tcctgcgacc atttcaaagc agatgaacac aaaactgaat gtaaaagtaa
    12301 tataaatctg ttcgtagttt ccttgatata cataatgccc gattaatcca gcaaaaagaa
    12361 ctacggaaat acaaaagaat attgaccaga caatactcaa tgtttgatca atgaagatga
    12421 tcgttcttcc aatatgttta aaaggatgga gcatcacttt tttgaaattc gtcagccaga
    12481 cttctgttcc accttttgcc caacgttttc tgtgtcgata tagcatcttc aacgtcacag
    12541 gtacttccat gaaaaagata atctcaggag caaaaacaga taaccagtca tttaactgat
    12601 gatcccaagc aatactgatg tcttctgtcg ctcgatcttg tctgaatccg cccacatcga
    12661 tcaaggcttc ttttcgatac atcgtatttg ctccactgta cgcataaaga ccaccgaaaa
    12721 cagctgactg tgttcgttta atgatgccca cgatacttga gaattcaacc gtttgagatt
    12781 ttgcaatcaa tttggtacga ttttggacat ccatatttgc agtgaccgca gcaatatgtc
    12841 ttgcaccagg gcgaatgaaa taattcacat acctaatcag tgcatctggc tccggtaccg
    12901 tgtctgcatc attgcttaaa atcaatttcc cttttgcaaa ggcaagtccg atattaaacg
    12961 cgtgggcttt tcctttattt ttttcgatcc gaaccacacg aagattagcg tattttttca
    13021 tcagtctagc taatatttct ggtgtttggt ccgttgaacc atcgtctgtc actaatacct
    13081 catagttgtg ataattcaat ttcgtcatca aatactcgat tgtatattcg atcacaattt
    13141 cttcattatg ggctggcacc atgatcgtga tgaacggttc gacactcaag ggaacatcta
    13201 cccattcttt ttgcttatgt ttgtataaaa acacataaca ccagacacca ataaaccata
    13261 caaatccgcc taaaatcgga taagaaacga gaaaaaaata cataatagac aaacaagtat
    13321 ctaagaataa attacctgta tgggtgattg agatcatttc agttccaccc cattttcttt
    13381 atacaaatct gcaatgaatg tcgtatctag atttttctct tcggagacag aataaaaacg
    13441 gaccgtttct ctctcttctt ttgttccaaa acgttccgta tacacttctt caagtaattc
    13501 ttgtctt
//
LOCUS       DACDHJ010000053        13228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDHJ010000053 DACDHJ010000000
VERSION     DACDHJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.054"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            110..1849
                     /gene="yfcC"
                     /locus_tag="IV223_001226"
     CDS             110..1849
                     /gene="yfcC"
                     /locus_tag="IV223_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8711525.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2006..3190
                     /gene="iadA"
                     /locus_tag="IV223_001227"
     CDS             2006..3190
                     /gene="iadA"
                     /locus_tag="IV223_001227"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8711526.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3301..3504
                     /locus_tag="IV223_001228"
     CDS             3301..3504
                     /locus_tag="IV223_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8711527.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3605..4267)
                     /locus_tag="IV223_001229"
     CDS             complement(3605..4267)
                     /locus_tag="IV223_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711528.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4280..5782)
                     /locus_tag="IV223_001230"
     CDS             complement(4280..5782)
                     /locus_tag="IV223_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711529.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6330..8009
                     /locus_tag="IV223_001231"
     CDS             6330..8009
                     /locus_tag="IV223_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8711530.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSWGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8136..8615
                     /locus_tag="IV223_001232"
     CDS             8136..8615
                     /locus_tag="IV223_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8711531.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8834..9514
                     /locus_tag="IV223_001233"
     CDS             8834..9514
                     /locus_tag="IV223_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8711532.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9522..10853
                     /locus_tag="IV223_001234"
     CDS             9522..10853
                     /locus_tag="IV223_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8711533.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10868..11830
                     /locus_tag="IV223_001235"
     CDS             10868..11830
                     /locus_tag="IV223_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8711534.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11941..12279
                     /locus_tag="IV223_001236"
     CDS             11941..12279
                     /locus_tag="IV223_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8711535.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12358..13065
                     /locus_tag="IV223_001237"
     CDS             12358..13065
                     /locus_tag="IV223_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8711536.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4195 a         2335 c         2894 g         3804 t
ORIGIN      
        1 attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat
       61 ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac
      121 aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt
      181 cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa
      241 gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt
      301 tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca
      361 attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg
      421 attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat
      481 ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa
      541 aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt
      601 aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc
      661 tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga
      721 cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct
      781 attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc
      841 attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt
      901 aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag
      961 cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt
     1021 gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa
     1081 aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca
     1141 tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt
     1201 agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat
     1261 tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct
     1321 tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc
     1381 tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg
     1441 aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc
     1501 gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt
     1561 caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc
     1621 actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc
     1681 aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc
     1741 acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt
     1801 tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc
     1861 tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt
     1921 aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt
     1981 atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc
     2041 agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga
     2101 acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt
     2161 actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg
     2221 ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac
     2281 agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa
     2341 agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt
     2401 gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat
     2461 cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt
     2521 cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat
     2581 caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga
     2641 aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt
     2701 tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga
     2761 tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt
     2821 gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt
     2881 gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt
     2941 gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg
     3001 agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat
     3061 tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat
     3121 cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa
     3181 aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt
     3241 atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca
     3301 atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa
     3361 gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca
     3421 ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt
     3481 tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa
     3541 aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat cattatcctg
     3601 ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact tcatcacagc
     3661 tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt tcatgggcga
     3721 tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt ccagtaggct
     3781 catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg actcgctgct
     3841 gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca attcctacac
     3901 gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta gtgatttcca
     3961 ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa gcttggaaaa
     4021 taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt gaacgatctt
     4081 gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa agaaaagtcg
     4141 tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg aaattcagtt
     4201 ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat tgttctgtcc
     4261 gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat cttaatatcc
     4321 ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg gctaatagtc
     4381 ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa acagcaaatg
     4441 gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg
     4501 gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa
     4561 gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca actcctaatg
     4621 caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta cttctggatt
     4681 ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta atcataatga
     4741 tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt ttagaaaaac
     4801 ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt gattgtaagc
     4861 taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc attttatctg
     4921 gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt
     4981 catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag ttgaatttca
     5041 tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc atttcataag
     5101 tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg
     5161 tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg tcatctattc
     5221 cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt gcagttgctg
     5281 acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc attcctccag
     5341 caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca gaactagtga
     5401 tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa taactcttta
     5461 cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta agagaaaaac
     5521 tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt tcctgttttt
     5581 taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct ttttgtgctt
     5641 gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa
     5701 tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt gtacttttcc
     5761 atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt tgataataga
     5821 caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa catgaggttc
     5881 tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga aaaaagtatg
     5941 tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta gatgctacag
     6001 agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt tagtttattt
     6061 gttgtataat aatggggaac agctgaatac ttatataagt attctggcta tagcgtttcg
     6121 caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc aactgtcaga
     6181 acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat agtgattttg
     6241 catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga catctttaaa
     6301 cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc ccattaggcg
     6361 gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat atttttattt
     6421 tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc gtaatccctg
     6481 attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta acacatggac
     6541 atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta ccagtattcg
     6601 ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag
     6661 gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt gaaacagtag
     6721 tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat ctgaaaacaa
     6781 aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct atcccaatgt
     6841 atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta gccttactaa
     6901 gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa atagcagatg
     6961 aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta
     7021 atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga aaagttgtct
     7081 tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa aaacttcaat
     7141 taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat ccagaagaac
     7201 tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa
     7261 acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc actactacgc
     7321 caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat cgagctggtg
     7381 gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat cctaatgact
     7441 tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa ggagagtatc
     7501 gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac aaaaatatct
     7561 atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa
     7621 ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc ggaaacattg
     7681 ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt gtcactatca
     7741 gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcatgggga ttcgtttacg
     7801 taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca atcgttgaga
     7861 aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc cgagatcaat
     7921 taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca gtcattatgg
     7981 aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc tctctataga
     8041 gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag catccctcgc
     8101 tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac
     8161 gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat gtggtagaag
     8221 aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata tgtaaaactc
     8281 tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg aacgatcact
     8341 tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt cctggaatgg
     8401 attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt ggaataaaac
     8461 tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa gaagaagtaa
     8521 ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat ttagaaaaga
     8581 tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa gctaagcaga
     8641 gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct gtattatctg
     8701 ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt agcagctata
     8761 ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac agagaaaacg
     8821 cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa ttgaaatgga
     8881 aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga tagacttaac
     8941 tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg accacatagg
     9001 agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg ttgagattgc
     9061 tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg gaaaacatgc
     9121 gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct atgaagtaga
     9181 cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag aaggaaaaac
     9241 gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg cagaagaatt
     9301 cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc cagaagatgc
     9361 agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt
     9421 ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa
     9481 aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca aaacatgatt
     9541 tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta agaagcatcg
     9601 caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg
     9661 tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc aaattaaaaa
     9721 aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg gacgttttag
     9781 gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg atgactaaac
     9841 atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac cgggcggaag
     9901 tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga ttcaatacga
     9961 ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc acctcttatg
    10021 atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg atcaaaaaag
    10081 atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat ttgcagcaga
    10141 cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg cgttttccag
    10201 tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt ttagggaaat
    10261 cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa
    10321 tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc atgccggtcg
    10381 tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg aaagcgatgc
    10441 agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata tctggaaata
    10501 tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg
    10561 tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta cttagcgaaa
    10621 tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat atcttgattg
    10681 aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta gacattcgtc
    10741 cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg tttattgaaa
    10801 acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct tagaaagcag
    10861 gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg ataaaatcat
    10921 tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag gattagccga
    10981 actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg
    11041 gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt
    11101 catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc agttggcaga
    11161 aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc ttgtgtgggt
    11221 aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga tgtttcctga
    11281 atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg tcggagatac
    11341 agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt cgatattgag
    11401 aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt
    11461 cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag
    11521 aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag agacagcttc
    11581 gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt ttgttcagca
    11641 gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca atgaattggc
    11701 taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa aagatttagc
    11761 agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata agagcagaaa
    11821 aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt
    11881 ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag gagaaagaag
    11941 atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga tcgaggcttg
    12001 ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa
    12061 gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca
    12121 gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc
    12181 aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga aggatttgga
    12241 ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa ggtcttttca
    12301 cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga aacgaaaatg
    12361 tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat tcttggatta
    12421 gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata cgatcaagtg
    12481 agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca aagtcaacag
    12541 ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta caattcaggt
    12601 aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc tgcgataggt
    12661 gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt cccattgaag
    12721 attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt ccccttgatt
    12781 atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat tttcgctttt
    12841 tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc gtctcggcga
    12901 gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc gatggcaaga
    12961 cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac attcctgcag
    13021 ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa agaagttgtg
    13081 aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac catttatttg
    13141 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
    13201 tttcatatac agttcatgaa ccgctttt
//
LOCUS       DACDHJ010000054        13213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDHJ010000054 DACDHJ010000000
VERSION     DACDHJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.056"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            211..2682
                     /locus_tag="IV223_001238"
     CDS             211..2682
                     /locus_tag="IV223_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8711537.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2785..4521)
                     /locus_tag="IV223_001239"
     CDS             complement(2785..4521)
                     /locus_tag="IV223_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8711538.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4518..5222)
                     /locus_tag="IV223_001240"
     CDS             complement(4518..5222)
                     /locus_tag="IV223_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8711539.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5353..5856
                     /locus_tag="IV223_001241"
     CDS             5353..5856
                     /locus_tag="IV223_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8711540.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5816..6154
                     /locus_tag="IV223_001242"
     CDS             5816..6154
                     /locus_tag="IV223_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8711541.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6175..7593
                     /gene="ffh"
                     /locus_tag="IV223_001243"
     CDS             6175..7593
                     /gene="ffh"
                     /locus_tag="IV223_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8711542.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7705..8283
                     /locus_tag="IV223_001244"
     CDS             7705..8283
                     /locus_tag="IV223_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8711543.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8287..8808
                     /locus_tag="IV223_001245"
     CDS             8287..8808
                     /locus_tag="IV223_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8711544.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8887..9441)
                     /locus_tag="IV223_001246"
     CDS             complement(8887..9441)
                     /locus_tag="IV223_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8711545.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9694..9969
                     /gene="rpsP"
                     /locus_tag="IV223_001247"
     CDS             9694..9969
                     /gene="rpsP"
                     /locus_tag="IV223_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8711546.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9981..10232
                     /locus_tag="IV223_001248"
     CDS             9981..10232
                     /locus_tag="IV223_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8711547.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10357..11148
                     /locus_tag="IV223_001249"
     CDS             10357..11148
                     /locus_tag="IV223_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711548.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11318..11839
                     /gene="rimM"
                     /locus_tag="IV223_001250"
     CDS             11318..11839
                     /gene="rimM"
                     /locus_tag="IV223_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8711549.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11829..12590
                     /gene="trmD"
                     /locus_tag="IV223_001251"
     CDS             11829..12590
                     /gene="trmD"
                     /locus_tag="IV223_001251"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8711550.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12726..13073
                     /gene="rplS"
                     /locus_tag="IV223_001252"
     CDS             12726..13073
                     /gene="rplS"
                     /locus_tag="IV223_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8711551.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         4373 a         2414 c         2733 g         3693 t
ORIGIN      
        1 ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca
       61 caaaatatta tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc
      121 ttaatgtgag gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat
      181 ccagaaaacg aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa
      241 aaaaagaaag taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa
      301 gaaggattaa cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca
      361 gtcgatccca cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc
      421 aatttgatct ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg
      481 acttttttac cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc
      541 aaaaagatct tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac
      601 gggatagaac aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag
      661 actggatatc aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag
      721 gctttattga caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc
      781 ggcagtttca ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg
      841 tatatttccc agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg
      901 attgcttctc ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc
      961 ctgctttttt ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca
     1021 atggaagcag cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca
     1081 ttagcattaa gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc
     1141 attgagacat tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa
     1201 aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca
     1261 gatcaattgg aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca
     1321 atggaagctg ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt
     1381 cctttttctt ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta
     1441 ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct
     1501 ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa
     1561 ccagtattga cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga
     1621 aaagaagcaa gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt
     1681 tctggagata atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct
     1741 gatcagtacg ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa
     1801 tatacagttt ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag
     1861 cgtaacaata cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa
     1921 gcagactgca gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg
     1981 gtcttacttg attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt
     2041 gtgaacaata ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg
     2101 ctctcactgt tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca
     2161 ttgattagtt tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa
     2221 aagcgaatca gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta
     2281 acagatatga tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa
     2341 acagatgttt ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat
     2401 aaaatctgtt caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt
     2461 aacttttttt cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta
     2521 tcacttttgc tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc
     2581 acattggtag aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc
     2641 gtagctaaaa aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa
     2701 agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta
     2761 aaacgcacga gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc
     2821 tgttccaaga tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac
     2881 aatcgctaac cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta
     2941 aaaacgctca aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac
     3001 agaaaacgat agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga
     3061 atattggaca gcattttcaa tcagattttt gataattgga taaaggatat ctgttttagt
     3121 agtaaatggt tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg
     3181 ctgataattt tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta
     3241 agaattctcg ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat
     3301 cgcatctttt tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc
     3361 atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga
     3421 gacgttgcca acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata
     3481 cgccacccct agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc
     3541 taatgtctgc cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt
     3601 cacttgatga atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc
     3661 aggttgatag cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg
     3721 agaatcagca tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa
     3781 ttgctcttct gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt
     3841 aatcgtctga tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt
     3901 aggattttgc accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg
     3961 atagatcaga taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa
     4021 gacccaacgg cgaaaactct cagttctcgg taaaaaaacg cttgtcggtt ctgcgatacg
     4081 taaaatggct tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc
     4141 attcaaagta gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt
     4201 gatttcggga cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat
     4261 ctttccagta gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa
     4321 agcagttttg ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg
     4381 tgcttttttt gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga
     4441 aatcaattgc cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc
     4501 ataaattttt ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata
     4561 gtcaccaagt actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg
     4621 tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat
     4681 gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct
     4741 ttcggtgtta gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca
     4801 gtgatttgtc caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt
     4861 tcaattcgtc tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg
     4921 gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta
     4981 gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc
     5041 atgccatcca tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat
     5101 gctaattcta atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc
     5161 aaattgaacg tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc
     5221 attcaaatct cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa
     5281 agggctattt tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag
     5341 gaagagcagt caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat
     5401 ggacagaaaa aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg
     5461 gtctgtccag aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt
     5521 ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat
     5581 gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac
     5641 attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac
     5701 ggaagttttt ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag
     5761 caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga
     5821 aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga
     5881 aaaacaaatg aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc
     5941 ggaagaatac gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat
     6001 cttggaggat tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt
     6061 attggaaaat atgactgcat atattacaga taagtacccg gaagataaaa aactacttga
     6121 atatgtacag caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca
     6181 tttgaaagct taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa
     6241 gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc
     6301 gatgttaact tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg
     6361 gcagaagtgc tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa
     6421 ttgacgatca cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact
     6481 gttatcatga tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc
     6541 aattatttaa agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga
     6601 ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat
     6661 atgggaactg aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa
     6721 aagaaaaacg actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg
     6781 atggatgagt tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc
     6841 gatgccatga ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt
     6901 attacagggg tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg
     6961 atccgctcag tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac
     7021 ttagaagtat tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg
     7081 acattgattg aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag
     7141 aaaatgcggg aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg
     7201 ggtatgggcc cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga
     7261 atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct
     7321 atgacgccag cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt
     7381 gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa
     7441 tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa
     7501 atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag
     7561 aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac
     7621 tgaaaatgtc tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt
     7681 actctaaacg aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg
     7741 cggcaatatc atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga
     7801 aaattgatgc ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt
     7861 gaagcattcg atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa
     7921 acaaaaatca gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa
     7981 gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca
     8041 gagtatcttt ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa
     8101 gaagtcccaa caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga
     8161 tttaaattta tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat
     8221 gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa
     8281 taaagcatgc gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg
     8341 aaaacggatg atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa
     8401 gctttttatg aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa
     8461 aaatatttgg agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac
     8521 gcacaattac aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa
     8581 aatctacaaa tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa
     8641 ccgctattgg aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta
     8701 ggggacggga ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac
     8761 acgacacgat tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa
     8821 taaaaaaggc aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta
     8881 tgctttttac tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa
     8941 gaaacttaat gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc
     9001 gatatatgct tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag
     9061 atggtggttt gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg
     9121 tttatgatcc caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa
     9181 tactgctggc acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct
     9241 cacttcctca cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc
     9301 attgtaaaat ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa
     9361 tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa
     9421 aaatccaatt ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac
     9481 aaaagataag taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag
     9541 attacttatt tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat
     9601 ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc
     9661 ttgtgaataa aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt
     9721 atgggttcta aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt
     9781 gatggacgtt tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta
     9841 gttttaaaag aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact
     9901 gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca
     9961 aagaaataag gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc
    10021 cgctagtcac ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg
    10081 agtacaatct aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg
    10141 ttgctaaagc aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc
    10201 gtttgaatat catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag
    10261 tgcagctttt ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga
    10321 aagcgtatcc ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt
    10381 ccatttgctc tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat
    10441 ctatcaagca gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct
    10501 tccaccactg aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag
    10561 ttgaaagaac aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat
    10621 ctcaatatcg cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat
    10681 cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta
    10741 tatcaatatg ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc
    10801 gacacaaatg gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca
    10861 gcaggttcaa gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat
    10921 aatatagaag gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa
    10981 aacagtctac cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg
    11041 aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa
    11101 gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct
    11161 gatctgagcc catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat
    11221 ttttgcataa ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc
    11281 taaaataaaa cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg
    11341 aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt
    11401 cccagaagaa cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc
    11461 tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga
    11521 aggacatcct tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa
    11581 agacaactct gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca
    11641 tgtattagaa gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa
    11701 tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc
    11761 tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact
    11821 gattgacgat gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg
    11881 gggaatccat catcggtaag gctgtagata aaggattatt agatatcaat atctctaact
    11941 tccgcgatta ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag
    12001 caggtatgct gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg
    12061 caccggatgt caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga
    12121 aaatggcaga agaattttca caagaagaac acttagtatt catttgtggt cattatgagg
    12181 gatatgatga acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc
    12241 ttactggggg agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag
    12301 atgtgttagg gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac
    12361 atccccaata tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa
    12421 atggaaatca taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc
    12481 aacgtcggcc tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag
    12541 aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa
    12601 aatcttcttt acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt
    12661 aactattacg atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga
    12721 aaaccatgaa tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag
    12781 cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac
    12841 gtatccagtt atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt
    12901 atacagtacg taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac
    12961 cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact
    13021 acctacgtgc attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcattgt
    13081 tatcagactg agaaagaacc cttggtattc aagggttctt ttttcttagc atgtaatttg
    13141 aacaccctga tccagttttt tctttaatta ttggtagggg agaataatgg attagaataa
    13201 atctacctat aag
//
LOCUS       DACDHJ010000055        13093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDHJ010000055 DACDHJ010000000
VERSION     DACDHJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.057"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(270..2444)
                     /locus_tag="IV223_001253"
     CDS             complement(270..2444)
                     /locus_tag="IV223_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8711552.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2460..4802)
                     /locus_tag="IV223_001254"
     CDS             complement(2460..4802)
                     /locus_tag="IV223_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711553.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4824..7367)
                     /locus_tag="IV223_001255"
     CDS             complement(4824..7367)
                     /locus_tag="IV223_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8711554.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7377..7775)
                     /locus_tag="IV223_001256"
     CDS             complement(7377..7775)
                     /locus_tag="IV223_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8711555.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7856..8140)
                     /locus_tag="IV223_001257"
     CDS             complement(7856..8140)
                     /locus_tag="IV223_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711556.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8164..9162)
                     /locus_tag="IV223_001258"
     CDS             complement(8164..9162)
                     /locus_tag="IV223_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8711557.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9155..9502)
                     /locus_tag="IV223_001259"
     CDS             complement(9155..9502)
                     /locus_tag="IV223_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711558.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9518..10168)
                     /locus_tag="IV223_001260"
     CDS             complement(9518..10168)
                     /locus_tag="IV223_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711559.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10179..11351)
                     /locus_tag="IV223_001261"
     CDS             complement(10179..11351)
                     /locus_tag="IV223_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8711560.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11370..12242)
                     /locus_tag="IV223_001262"
     CDS             complement(11370..12242)
                     /locus_tag="IV223_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8711561.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12264..>13093)
                     /locus_tag="IV223_001263"
     CDS             complement(12264..>13093)
                     /locus_tag="IV223_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711562.1"
                     /translation="ENEELLARAEIGWCTLEIYTENHEIPVFEGSYDFPVEEFHLISL
                     IEQSLTENGQLSEEAQTFIQTLNSELPKFYQVKKDQKNERRPTKIKKEKKTPLSVETA
                     FTQSNGSLQKKKMSFQWLLFGFIVVVSCGLFIMQLSTQREIEQLTKKEHQLQERIAFA
                     EELATKQPQIDTFSRYFITNYYSGEMAEERYRKKLQSFLTEKVAKQISPQKEKVKSIL
                     SWEMKKEGKHWQIAYVITLETEKKTQETKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
BASE COUNT         3567 a         2839 c         2107 g         4580 t
ORIGIN      
        1 cttgtcttgg ttcttttctt gtttcaattc gatatacatt cctagagaat tttttgtcgt
       61 atctttccaa gaaagattca acttcttggc attttttaca atcatttctt gctagataca
      121 caaaaaatga ttgattcgtt ttttgaagtt ctagtacttc ttctaaatta ctattgatta
      181 gttttccatt tgattcttcg ttagttttta tcagtttaaa acaaccaaat aagaggaaac
      241 taacaataag tatcctaaca ataactttct tatttcgcat attgtttctc caaatttggt
      301 ttccgaatat agacgactcg cggtggttcc tggacgtctt cagctgttcg atacaccaca
      361 aattcgcctg ttctcaatgc tcgaatgcga ttcggattga atttaaagcg gtctacgtct
      421 cgtttcgtgc ctctttctcc tgtccaatca acctttccca tatcagaata acctgcttct
      481 tgtgtcgtca tttccgttaa ttcaatgtct tcttctgtcc ccattaaatc tgcccaataa
      541 tcaatttctg tcgggtcatt tgtcttccca ataaagaacg tattcacatt cccaatcaac
      601 tgttttcgaa ggacaggatc aattctatca atgtccgcaa gcgtttgagg ggcatacgcc
      661 gcatgcaccc cagcgcctcg tcccttattg actaagtcta taaggttatc atttagataa
      721 gaagctggtt cgtcaaatat tactaggact ttcgagcgat tggctgtttc tcctgcttgt
      781 ttatacactt ctgacgcaaa gaaattaata tctcccacaa gcatttgagc caatgtccga
      841 ataaactcac tataaatcaa tccgtttaac gagatataaa caatctttcc ttctttaaca
      901 acttcttgaa tatctaattc tctttttcct ccagaagtat caaagagttc accaagctca
      961 ctatccaata acatattgat attggtctgt aaattctcag aagtcgcata caaataaaac
     1021 gggttcttct tatgttcgta acgaacaaac aaccgtttaa acaacgtttg ctcgatttct
     1081 gtcatgagag aaatgttttc ttttaataat agataatacg tttctaagtc aacggtttta
     1141 cgatcatgtt caacgattcg ctcttcactt aactgttcca catcggcaaa atcacgttct
     1201 gttttgtcca tcgcttcatt ctttttcgtt tttttcttcg tggttttctt cttttctacg
     1261 accacttttt ctttaaataa atctggattt ttcaactgaa tacgatccgc aaacaaatgt
     1321 aagacgtttc gtggcaataa ataatgttgt aaccaaacta gatttctttc aattttatgg
     1381 ctcttttgga actcgtctaa aagctgaatc gttacctgta ataacgcttt ggctgctgtc
     1441 gaataataca cgctttctgt ttctgctaga ttggttaatt tatctcgaat ggcaatatca
     1501 ttgccatttt ttacaggatt ataacgcaaa tcgccaccgt ccacaaagac ttcaatcggg
     1561 cgattgtatg cctctgccac ttcttttaac gcttgtaaag tctctcctgc tcctttccca
     1621 tcgactaaga taacggggat acgaaactta cacgcatgtt ctgctagtaa ttggatcacc
     1681 gtcgtttttc ctgaacctgt agtcgctgga ataatcacat gttggttcaa ttcactaaag
     1741 ttcatcacat aagggagccc atactcatct gttcctaata gaatcacttt ggctaatgaa
     1801 gccagttgtt tattccgcaa agcaacgtct tttatgacaa ttgctacctg gtacgcttgc
     1861 tgatactctg ctgtatattt ttgggactcc tgaaatttat tcttccgacg ttccttgtat
     1921 tctttactat ttagcaaagc tgtttgccgt tctttctttg aaacaacttt tcttttttca
     1981 ataaaagacg catacgcaat caaacaactt cctgcacctg tcccacctaa aaggagggca
     2041 aaaatcgttt gtccattaaa ctgcaacgca tgagaaaaca agtttaatgg aaataataag
     2101 taacacgaca aataccctct aggaagaaca aaaataaaac aaaatagatt taatagcgta
     2161 aaacctaacg caaaaataaa aaaaagagag cttcttcgtt ttaagaagct ctcatactca
     2221 aagtcataaa tatgtgcacg tgtctctttt ttatctatca taaagacaat cgctaaagga
     2281 atactgccta caataaaaaa tggatataga caaacaccta ataaagtgac gataaaataa
     2341 agttgttgaa accgactttt tggcgtttct ttttcttgtt ttcttttgcg atcgacagaa
     2401 aacatttgct caaaatgtgt cggtcgtttt ggtgcattca tcatactatc gatttcctcc
     2461 tatcgttttc gttgtttcat ttctcttcga taaatcggtt gtttttctcg ttttcgtttc
     2521 atttttgctt gagttgctcg aaactcactc cgtgtcttcg gtgcttgttt tggtgatttt
     2581 gtttgtttca tggttgagta tgctttagat acggttgaag gcgctttggt ttgtgctgtt
     2641 actcgattga ctgctgacat atgttcccga accccctctg gacgtcgaac agatttcggt
     2701 gtggtagaac ctgttgctcg tggtacattc ccttttctta gtggtttttg taccgcatgt
     2761 ttttgtcctg caactgtccg ggttttcatc gaacttgttt ttgttacggg agtaagtgcc
     2821 tccttccgac gacgacttgt ttgtccctga cgacgttttc gatcgttcaa ttctgttcta
     2881 ggcgtatgct tctggccata agacttgtgt ttttgctttg gattcgctaa tgacaccacc
     2941 attgcagaac cctgattgcg atacggacta cgagacgcac cttttctact tctttctctt
     3001 agactaggat tgccttgcaa ttctcgttgt aataaatcgg ctgtttttct tgcctgtttc
     3061 tccattgctg ttcgttgcat acgttcttga ctttgctctt ctgcttgttt cgcttgatca
     3121 tgcaagtctc ttgctttttg tgccatacgt tccatacgat ttggtgcttt atgacgaggt
     3181 tgttgtgcgg attcacgccg aaaagcactt cgtgtggctt gtttcgccgt ttgtggcata
     3241 cgaacgtctg agccaccccc acgttttcgt tgacgttctt gtctcttccg tgcgttttct
     3301 ttttcttttt gtttctgtgc ttttctcgct tctttgcgtt ctttcaaccg ttgtttggct
     3361 tcccttctca attttgcttc tgccccaaat ggatttcccg caaaagctaa cgctcctcga
     3421 acacccattc ttccagtaaa tagactgcct aaaaagcctc ggaacaaata aattcccaac
     3481 ggcgcaaaca aataaacaat gatcaagaga attttttccc atggatcgtc ttgtgtacta
     3541 cttaattgga acccgtaact aagaaaagaa gaaaaaagga tacatgcaaa acctgtcatt
     3601 ccttttaaaa acgtcaaaat accaaaaact ttggcatagt taccaaacac attcattgtg
     3661 acaagaaaca atcctaaaaa aagtaagaac ggcaacatcg gtactaacaa tagttgcata
     3721 gttccaataa tcaatcgaaa catacttaag acaaaataaa caacggtttg aatcagattc
     3781 acgacaaaat aaaacaaggc aaatgctgta tttttccaac tattgtaata ttgtatgttc
     3841 ttgtttttca gtgtatctgc ttcatactct gtgactttgt catgaatttt ctcccctatt
     3901 tttgtaccta aatcattgtc caataaaaga ttgattcgat catattcttt tccagcatat
     3961 ttgactttct tctcacgaat tgtgtctaca ttggttgtgc cataatttaa caacagatat
     4021 ggttcataaa cattactata aaagacccga gccgccatca atttgcctgc cgtttccact
     4081 tttttatccg ctgttacctc ttctgtttct tttgtttcat aatttgtttc ttcttcaggt
     4141 gttaacactg gattgacctt cacgaacgcc gtttccactg tagaatctaa agaaaaaaga
     4201 acatcaaaaa gattcttatt tgtcccagaa tcttttaata cgcttaaccc agtaaaaata
     4261 aggatcgtca taagaaagac acggagtgct tgtttcattc gagcctctcc agcatatttg
     4321 attgccatca taattgccga aaaactgatt ccaatagttc ctgcaatact taacatattg
     4381 tccgcaattt tttgtgttaa gttttgaatg ggttctcgaa tggcggaaat aatatctaat
     4441 tcaaagagaa attcaaccat tgaagcattg aattctccta acatttttac gccactccaa
     4501 acaaaatttt tcatcactcc tgcaacattg tacgatagct tttttttaag gttcattgac
     4561 tcatagtcat tggtcatcaa ttcaaacgaa ttctttttgt aatttttata caattgatcc
     4621 acatcagtcg ttacttcttt tacttctact ttatcgttgg cattctctcc ttcagaacga
     4681 tcggtttcag tgacacccgc accatcattc atttgatcgg cagaaacagt agccccttgt
     4741 agacagaaaa aaagaccccc taataaaagg agcgtccacc atttcagaga tttttttcgc
     4801 aaaaaaacca cctctttttt gttttatcgt tgtttttcat tttctaacat tgcttgaatt
     4861 ttttcatctt gattttcagt agaagaaaca gccaataacc aactatcaaa caacacatca
     4921 atggcaatcg gttgatttct tcccaaatga tcctggaata aacaggttcc tgtttgtaat
     4981 tgttccatca tttctaaatt ggctgaattc acttccatat caaagaagga caatagtttc
     5041 tctcgtgctt ctttttgttt cggacgaaac gcaaatttgt aagaaattaa ctctttttta
     5101 tcttcttgat cgtaatcttt aaacgcttgc acaacaaggt aaatatccgt attattcgca
     5161 cgtccttttc gcaagctatc atcaatcaaa tagcttcctt gtgcggtatc ttcataccct
     5221 tttgcctcgt cataaataat cgcagcgtct tcttctttgt ttgtagaaaa gatatacgta
     5281 aacttcataa tgacttccat aattgccatt cctgcaagtt gttcgctatt taatcgaccc
     5341 gaatcaatct ctttttgtgt cggaatgcgt attccttgtg tccctaacac gttgatttgt
     5401 gaatcaaaac gtatcggcgt actgtgctca ttcccaatga gaatctttcc taaaccaacg
     5461 ctatgcccta aaatcaattt agctagttct ttgtcacttt ttcgaatttc ttcgataact
     5521 tgacttaacg aatgcttacc tggcatggtg taaactacat cgatggcgtc ttggatcacc
     5581 gttttcttgt ggctcgctgt ttctcgattg gtatctactt ctcctaacat tcctaacaca
     5641 tttttagcgg tttgaattgc ctcttctttt ggtaaaaaca atagaggatc aagcatccct
     5701 cgacagcttt catctgaaga taacgaaata aaattaattc gttcatacat agccacaaac
     5761 tctggtatgt gtccgaattt ttcaagtgcc tttaaaaaga acttttgggt ttcatttttc
     5821 ggatcaatat aaagaatttt ttgacctaaa aacatcagcc ataaaaaaat atattttaca
     5881 aggaccgatt ttcctttacc tggtggacca ataatcaacg tatttccatt ggtatgttcc
     5941 gcgccttcaa tacttttctt tgttaaattg ggataatacc atactagttt actagatagc
     6001 gctaacgctt ctttcgcaga tttaaacttt tttgcaataa caatccgtcc taaaggaaat
     6061 cctttcttat ttcctacttc tttctttaag tccaagccat aatctgccac aaaacctgtc
     6121 gtagcgacct gctcgtaact acgaaaacgt tgcgtacttc caattaagca ttgattaaac
     6181 aacgttagct gatccgctac cgatcgataa aatttgaaat ctgtagactc aatcgcaaat
     6241 tcaatatctt tgactcgttc ttccaattct tcttttgaag aagcagatac aaccagcgtg
     6301 acggtagaat aacacaatcg tttttctttg cgcttcagct tatcttttac atccattaac
     6361 cgttctgtac caaaatttaa tacatcgtct gaatctaaaa tgccgtcaac ggtctcatat
     6421 tctttttctt gttcataaat ccgtccagcc attttattaa tttttcgttc atcagatttt
     6481 gaatgctcaa atcgaagtcg aatatgcgta tcaattggaa aagataagct atcctgaaga
     6541 ttttgaataa agctactccc atacatggct aaaggagcgt ccgtttgact taaaaacgtc
     6601 gaataatgcg tttgattcaa ctgttcaatc gcaatgtatc ctgcttcatt cgaaatgacg
     6661 ccctcgcaaa tttccattgg cgtaagctgt ctttcaggca atttttcttc tgatcgatgg
     6721 aaaaaataat aataaatttg tccaatctct tgggcagata agcgctctat cggcttataa
     6781 ttggacaatt ccgtatagag ctccttttct aatgtttcat acgttggtaa cacactactt
     6841 ataggaactc gttgtcccgt aatacgatag ataaactttt taaaaacatg cttaagcaat
     6901 tgaaaatatt cgatcggatc agacactttc atttctgtag tgaaacgcac aaacagatac
     6961 gttcgatact cgtttaattg cacttcgtct tttaaaatct ttcctgcctg ttcaaagtac
     7021 actttcccaa gatctgcgca ttctcccttt accaaatctc gaatggtttc ttcaatgtgc
     7081 gtatctaggt taaattgacg tggaatattg ataaaatgat agtcatattc atcatgtcca
     7141 agtatgcctt cgccatcttg aagatagttc ttgaaaaaat caatgtcatt taaaggaaat
     7201 acttgggaag aaagttgata gcctacccac acatcatcat ttttggtcaa aacaaaattt
     7261 cggataatgg cgtctatcgg aaattctaat cgaggaagct tcgttttttt ctttttgaac
     7321 tgccaaatcc cgataataaa gacgaccaaa agaaacaaaa acgccattta ctcgactcac
     7381 tccttccatt tgtgtaggga atacgtcact ttttcatgga aaaacggcac atacatttca
     7441 tgttcaaagc gttgtttccg tttctgataa taggacgttc gcccaattaa atactcgaaa
     7501 aagtgtttat tgtcccattt cattgaaaac aatgtccaaa cgagaacacc aatcccaaaa
     7561 atcataatca accaggactt tcgaaacagt gtctgcaaga aactaatttg attcacatac
     7621 gcaatcaaaa ataggatcaa aagaatgaag agaaataccc cacctatgac taattgttcc
     7681 atgcgaatgc cattagaaat ccagggaatt ttttgtcctt taatcccgta aattttgtga
     7741 gggtgcttaa actcctttgt atagttgtat tccatgttca ctctacctcc ttttacaaaa
     7801 aaagaagcga ccagaagttc taatcttctg atcgcttttg cagtttgtct ttttcttatg
     7861 ataattgtat ccagggcaca atcatttgcg ttacccccac gttctcttca ctatccttaa
     7921 taaagataat gttctttagc cattcaccaa tatcaacggc attcgataac aataagacga
     7981 gcattatcaa aataaagacg ccaataatgg caaacgctgc ccgcgcaaag ccttgggtcg
     8041 cataagcacc aaccattaga acaatcgcaa cgattgccaa tagccaaatg acttgtccag
     8101 atacatcatc aaataacgat ttaagatcaa aaagtttcat ttccattctc ctctaagttc
     8161 attttattca tttaacgtag taacaaaata actatcgcca cttgttttta cttccattga
     8221 aaagtttgat tggaactcgt ttcctttctc aaaatagaaa cgatacttcc cttgcaaaaa
     8281 gtagtttcct tctttatttt ttactgcatg atccagttgt acattgatca attcgccgcc
     8341 ttctaaccct ttttcgttag aaatcaatgc caatttttca tcatttttga catataactc
     8401 aaaaaaccgt ttcacaaatg cttccagctt gatttcttcg ctttcagtga ccgcttgccc
     8461 agaagaataa aatcgatttt cctgatagtt cacagaattg gtattcttac tctcagaaag
     8521 attcaaaaat tgaacgccat caagtacttt taaaacattg ttttgataac tgactggaaa
     8581 agaaagttga ctgcttttcc aatgttctcc ttctgtaaaa gtaacctcat aaaataggcg
     8641 atacattgct tgtgtcaaat cgatttcttc tagtttaata aagcgaacat ctttcgctac
     8701 tcgattggta gccgtcgtta caaagcctag atcagacgct tccaatcctt gtgctaaata
     8761 atgattgacg gaagcctccc attcgtttcg tttttctggc gaataagtaa ataacctttc
     8821 aataaaatgt tgtccctcgt acttcaattg ttccgtagaa gacaaggttt ggttgacttc
     8881 agttaacaat gtttctggat tgacttgttt tttcactgct aaacgcgtta atgtatccac
     8941 tcgtccaaag tttaggagga gcacgacacc cattaacaga aacaaacaaa aaaaagcgcc
     9001 tgaaaataca ctcatggcac ttttttgcga aaattggatc attcgatctg acggttctgt
     9061 tttctttttt tggaacaaaa aacgtttctt ttgtttcttt tccttctgtt gtttcggtgg
     9121 cttagtttgg aacgcttgtg gattaaattt tgcattactc atgagaaatg ccataccgtt
     9181 ctaacaataa tttcggtatt ttgtccacaa tcactacttc gtggacaacc acacttgccc
     9241 cctccaaaaa ggcaagtgct tctttgtagg cattttgact gtattttttt gataaagcat
     9301 gacaaatttc ttgataaaat agttgactgc ctaccgaata ccgattattg tgtatccgcc
     9361 acaaaacgaa cacactgatt tgaatcgacg ggcgtaaaaa agaaaaattc tttccataag
     9421 aaaaagaacg tccttcttgg gtttcttttg cttgttctgt tggtacttct tgtacctctc
     9481 ttattgattc ttgtctctcc ataaccttct ccttttctta ttcttctaaa agctgttgat
     9541 taaagctact tcccaaattt tgcataaacg tgattttctt tgacgctgga tcaaaggtta
     9601 cgtcaaaaat attcatggca ttattccagg aactatcacc aattttattg cgatacgaca
     9661 ccacaacgag cccttggata gtcgcttttt gtgtgctttt taaataaaca gaacatttct
     9721 ctaattcaga agacaccccg tttgaatcac cataggaaac gccttctttg aaatattgct
     9781 tcatcagttt gtcagaaaga tatggtgcac aattgttttt tcgttcttga aaagaatcag
     9841 gctcataatt aaacagaacc tcgaacgctt ctgtcactgt tttttgaaag gtttgaatgt
     9901 ttcctttttc tgttgaatca ttgtttttca attcttcatt tgcttttttt aacgtcgtga
     9961 cctcgctttc tacttgttga agttgttctt tgagtgacgt tacaaggtct tcctgtcctt
    10021 tcttctcttc ttggaacttg tttatctcca ccgtcagttt ctcttccttt gctgttagct
    10081 gtagcttttc atgaataaga aaaaatgtac tgcctacacc aaataagaac aatccgccta
    10141 cacaaacata aataaatcgt ttcttcattt gacacctctt atttctttat cggtcgagca
    10201 aaacaaatat caccaccatt aagcaaatac ccaaaggata aattgacttg cccttcacct
    10261 acaccgttag tattcattcc cccttgttcg attactttcg tagaaagtcc atgccaatct
    10321 tccgctaaaa cagctgtgtg tccatcattt cccacaccag aacctaagtt cacaatgata
    10381 atatcgccag cttttgcctc gctctcaggt atttcttgta agtattgctt acttcctctt
    10441 gcgtcgctcg tcattgagcc ggtaaaccac ccaacgttcg caggcgtttt ataccctgct
    10501 ttcgttaaaa caagccacac aaagctcgaa caatcggcat agccattttt atctggattt
    10561 tcgacactac caaattgtgc ccgttgcggt tgtgaataat gaaaataacc aatcagactt
    10621 tttgctacgt ttaatacatt actatcggaa gaactgcttg ttcctgttgt ttctagacaa
    10681 taatcggtca agtctttact tcctgcgttt gccccatttt ctaatgtaat tgaagcgacg
    10741 ggatcactgt cattctttcc tagttttgcg taccattgat tcgcaaaagt aatacgattc
    10801 cccatcattg gtatccctgc acgctcaaat ttcacttgcc actgttctgt tgcttcctct
    10861 acgctttttg cttgtaatgc aacttgtgaa gctttttctg ttgtgtttag ttcatgaatc
    10921 aaaaactcaa cttgtacacc taaatctgac cactctttgt ttcgctcttt ggcaaaattt
    10981 ttcaaagcag tcgttcgccc tgccgtccat tgtgcaatcc catgcccaga taatggatcg
    11041 tttggtcgtt caatcgctgt gggactcata ctcgactctt gttgaaaatt gcctaacata
    11101 cccgctagac cctctaatgt cgtatttgga atatgttttt gtacttcctt tgcaatttga
    11161 agcccgtttg tctccatgcc ttggttcgtt aatgagatag tacttgcgac aatttcggtt
    11221 ggactacagt cgatttcttc ctcttcagaa ccactactaa tcaaaagtat cataaacaga
    11281 aaaccaaaca tacatagtaa tagtgtgggt agacaaccga acccacacca taaaagaagt
    11341 tttttcttca tgtttgatcc ttcattcgct tagttcattt tttgtgttgc ttttcttttg
    11401 cgccaaaaag caaaaccgct tcctgaagcc aataatccac caagtgctgt cgcaacgaga
    11461 gacacttctt cgccagtctt aggcaatact ttcatttttc catcagaccc tttcaccgca
    11521 atatttccgc ttggaagttg ttcgaccttg tctgaaatgg gcgtcaagcc atttcctgat
    11581 tctttataag cgacaccatc aacgacggag acaatttttt ctcctgaagc tgtttcaatt
    11641 ggtgtacttg gtgttggaat atgggttaat tcagacgcat tattggtttc aatttgaaca
    11701 ttttgtccac ctgtttgttg gctgccaata cttccatctg gttttactac gacgtcctgt
    11761 actggaggcg tagtgttcgt ccccccatta ttggtaggtg gtgtcgttgg aggagtcgta
    11821 tttccttcat tgcctcctgg attcgacggc ttttctggtg tctctgtatt accaccattt
    11881 cctgggtttt cctcaacggg tggaacttca ggtgttcctt cccctccgcc agtatctgga
    11941 ttttctggta cttcaggcgt ttctgtgcct ccacctgtat ctggattttc ttccgttggt
    12001 ggaatctctg gggtttcagg agtttctggt atttcgggaa tttctggggt ttcttctcct
    12061 ccatctgttg gtggtaaaac aggttcttca ggaacttcag gtgtttcttc cgttggtgga
    12121 ataatttctg tcggtggtac acttggatca cctggtgacg tagtatctgc atacgcactg
    12181 gttcctacca tacctagcaa tacagttgca ctaaataaac ttcctaaaat catttttttc
    12241 atgtgttttt cctcttctct tatttattga tttatttcaa aatcatctag ttcgggaagc
    12301 gtggaaacta gataattttt ttcctgttct tccaattgaa aagacacttt ttttgtttct
    12361 tgtgtctttt tctcggtttc caatgtaatg acatacgcaa tttgccaatg cttgccttct
    12421 ttcttcattt cccaggataa aatacttttg actttttcct tttgaggact gatttgtttc
    12481 gcaacttttt ccgttagaaa cgattgaagc tttttcctat accgttcttc tgccatttca
    12541 cccgaataat aattcgtaat aaaataacga ctaaatgtat caatttgtgg ttgttttgtc
    12601 gcaagttctt ccgcaaatgc aatgcgttct tgtaactggt gctccttttt cgttagttgt
    12661 tcaatctctc tttgcgttga aagttgcata ataaaaagac cacaagatac aacgacgata
    12721 aatccaaaca aaagccattg aaaagacatt tttttcttct gaagagagcc gttgctttgt
    12781 gtaaacgctg tttctacaga aagtggtgtt ttcttttcct ttttgatttt tgttggtcga
    12841 cgttcgttct tttggtcttt ctttacttga taaaacttcg gtaattcgga atttaaggtt
    12901 tgaataaagg tttgtgcttc ttcacttaat tgcccatttt ctgttaaaga ttgttcaatc
    12961 aatgaaatca aatgaaactc ttctactgga aaatcatacg agccttcaaa tactggaatt
    13021 tcatggtttt ctgtgtagat ttccaaggta caccagccaa tttccgctcg tgccaatagc
    13081 tcttcatttt ctc
//
LOCUS       DACDHJ010000056        13024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDHJ010000056 DACDHJ010000000
VERSION     DACDHJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.058"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..986)
                     /locus_tag="IV223_001264"
     CDS             complement(150..986)
                     /locus_tag="IV223_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8711563.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(997..1926)
                     /locus_tag="IV223_001265"
     CDS             complement(997..1926)
                     /locus_tag="IV223_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8711564.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2118..2471
                     /locus_tag="IV223_001266"
     CDS             2118..2471
                     /locus_tag="IV223_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8711565.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2545..3201)
                     /locus_tag="IV223_001267"
     CDS             complement(2545..3201)
                     /locus_tag="IV223_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8711566.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3427..5361
                     /locus_tag="IV223_001268"
     CDS             3427..5361
                     /locus_tag="IV223_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8711567.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5447..6670)
                     /locus_tag="IV223_001269"
     CDS             complement(5447..6670)
                     /locus_tag="IV223_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8711568.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6664..8562)
                     /locus_tag="IV223_001270"
     CDS             complement(6664..8562)
                     /locus_tag="IV223_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8711569.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8556..9674)
                     /locus_tag="IV223_001271"
     CDS             complement(8556..9674)
                     /locus_tag="IV223_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8711570.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9757..10590)
                     /locus_tag="IV223_001272"
     CDS             complement(9757..10590)
                     /locus_tag="IV223_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8711571.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10583..11500)
                     /locus_tag="IV223_001273"
     CDS             complement(10583..11500)
                     /locus_tag="IV223_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8711572.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11727..12851)
                     /locus_tag="IV223_001274"
     CDS             complement(11727..12851)
                     /locus_tag="IV223_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8711573.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4068 a         2606 c         2164 g         4186 t
ORIGIN      
        1 tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa aataaatatc
       61 aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc gtgtcagctg
      121 tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata ctgatttttc
      181 ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg cttgaatggt
      241 tgataatagt ctgattggat ggttccttct attttatacc attcatcatt tttaaccgtt
      301 actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac acaatggata
      361 atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg gtaaacaaat
      421 ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat tagctccata
      481 acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata cttttctttc
      541 aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt atccggcttt
      601 aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg gaaattaaat
      661 cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt ccccacgatt
      721 actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga caacccatgg
      781 tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca caaatacagc
      841 tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta gcgataatgg
      901 acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa ttcgaaatac
      961 ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg tgaatccaat
     1021 aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa aatatcgttt
     1081 catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg cagctacagg
     1141 tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt ctgagcaaag
     1201 agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg ttgagccgag
     1261 cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta atccgccaat
     1321 gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca catgcccagt
     1381 ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt catggacatg
     1441 atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag cgtctttttg
     1501 aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta tcacccgcca
     1561 gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca cgattggatt
     1621 gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc cttttttaac
     1681 aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa atcctaagat
     1741 cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca cacgttctgg
     1801 ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga aaggtaatgc
     1861 ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg gaataaaaga
     1921 aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat acgcttttat
     1981 tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta ccaaagcact
     2041 tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc acttctatca
     2101 taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa gacatactgc
     2161 aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt tgtcacgctg
     2221 ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt catttggatg
     2281 catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca gttgtcctga
     2341 tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt aaaaaaatcc
     2401 gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa gaaatcttcc
     2461 gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa gttttgcctt
     2521 catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg catttgcgtc
     2581 agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat tttggatcaa
     2641 tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt tgattcctgc
     2701 ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta aaattgctac
     2761 ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa cggggatacc
     2821 gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga tacgtatgct
     2881 attactttga tggaatttgt attttagtaa agcacttccc aagttcccta caccgatcag
     2941 cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca taagatcttc
     3001 tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat ctcgacggat
     3061 cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt ttttctttcc
     3121 agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag ctgtcgcctt
     3181 tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac atctttgatt
     3241 tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat attgtgaaat
     3301 tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat ggaatttatg
     3361 gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag tagaaaagag
     3421 gattccatga tattattaca agccaatcag attgctcgcc attttggtgc agaagtctta
     3481 tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt tggcagaaat
     3541 ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc tgatgaagga
     3601 aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac aggacttgat
     3661 tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag acaaatggaa
     3721 gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac acctgcttat
     3781 agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg aaacggctat
     3841 ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc ttcattttat
     3901 gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt agctcgcatg
     3961 cttttgcaac accctgacat tttgattcta gacgaaccaa cgaaccattt agatatcgag
     4021 acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt gattgtttcc
     4081 catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag cagaagaaaa
     4141 atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga acagttagca
     4201 agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga agacttcgtt
     4261 gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag aaagacatta
     4321 gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa ctttctattc
     4381 agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc tatcggatat
     4441 gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga agcgattgct
     4501 ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat tcaccagatc
     4561 ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta ttacgatcaa
     4621 ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga tgagcatcct
     4681 accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc tggcgaagat
     4741 gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc tttagccaag
     4801 ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca tttagatatt
     4861 gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact gctttttgtt
     4921 tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact ctctgaaaat
     4981 ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca ggaagaagaa
     5041 gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc cacgaacaaa
     5101 tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca acgaaaaatt
     5161 actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt ggaacaagaa
     5221 atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa ccaagattta
     5281 gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact aagcttggaa
     5341 ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg ctgttttggt
     5401 ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac caataagaag
     5461 accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc caaatacaag
     5521 gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa aagccatctt
     5581 cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa acagcagact
     5641 ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct aataatccac
     5701 aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca tcaaagatca
     5761 aatcccaata acaaatggta tcttctggca accgattcat aaaataattg gctaatattt
     5821 tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca aaaccgtaaa
     5881 tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga tgcgtacttc
     5941 catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt gaaccatctt
     6001 ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt ttatattttt
     6061 gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa caatccatga
     6121 tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata actcctgcct
     6181 tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc ttcatttttc
     6241 gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa ccaagatcgt
     6301 gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct gctttttcag
     6361 ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta attcctgtcc
     6421 aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt ataggacact
     6481 cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc gtgtgtctgc
     6541 gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa cattcattta
     6601 tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt tccccaccag
     6661 cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta gcccggttgt
     6721 cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat cccgatcttg
     6781 atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac cctctaatcc
     6841 aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca taatagctgt
     6901 ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc cagcttcact
     6961 caccctatag ttttccttgt cttcttctat gattattcct tctttctcag gagaacattc
     7021 aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt tgtgtacgcg
     7081 aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta aagtaggttc
     7141 ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat ttcgaacgat
     7201 gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat atgtgtccgc
     7261 tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat tcgctcgcgg
     7321 tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat tggtcaatgt
     7381 atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat tatcatgttg
     7441 tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca taggcatttc
     7501 tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa tcttattcag
     7561 ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag ggtaggtata
     7621 accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc atcttatatt
     7681 ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg cccacaacat
     7741 agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa aaggcacatt
     7801 tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag ctcggttctt
     7861 atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac cataataatg
     7921 aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat accagccatt
     7981 ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt ctttaactgg
     8041 ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc aattaccatc
     8101 tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt tttctcctgt
     8161 atctaatttt ttccataata attgttcatg atgatataac gctaataaca ttgcgggcat
     8221 ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat gggaaggatc
     8281 tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta attggtcaat
     8341 tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa ttttatttcc
     8401 atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat aaaacggctg
     8461 gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc ttaatagtgg
     8521 tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa ttccgactgt
     8581 tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg cacattataa
     8641 gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc atccatattc
     8701 caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc atgatacatt
     8761 tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc agcgcatact
     8821 ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg aatataacca
     8881 gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc tagatagtac
     8941 ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc atttcctctg
     9001 ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata tttccctgat
     9061 tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag atacttagct
     9121 gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt ttccctcgaa
     9181 gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc aggtttatgg
     9241 tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag taccgtagct
     9301 gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg tgcaatagcc
     9361 gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta cagggcagat
     9421 tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt tgtcttgtga
     9481 aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat tcctctgtcc
     9541 tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt ccccattctg
     9601 tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga ccattccttc
     9661 gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg gagttgctcc
     9721 tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag ctccaatgtc
     9781 attctcctta aatttagact gcgcccacac ataaatcaag accattggga tcactgaagt
     9841 tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag aaaccgtcat
     9901 atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga ctaatggaaa
     9961 tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa tcgtaggtga
    10021 taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat cgattctggc
    10081 agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa acactgaagt
    10141 tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc ctaaacgact
    10201 cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag atagcataac
    10261 tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat aacccgccat
    10321 tgtagaactg gctgtagctg aaactgtact gattacagca ataacaatcg aattccaaat
    10381 aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata gcatatcccc
    10441 aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg aagctacaat
    10501 catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga cgtacgtaaa
    10561 aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta aattgaatag
    10621 ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct tttccaaaat
    10681 caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct gaattttctg
    10741 gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag ccattgatag
    10801 tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt aataaaaaga
    10861 attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct ttagaaatcc
    10921 cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa atcatcgtga
    10981 tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg ttaattccta
    11041 gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg aatacggaag
    11101 ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa aatccttttc
    11161 cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc atcgttattg
    11221 gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac aatggatctc
    11281 ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca attttcataa
    11341 aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag aaaataacaa
    11401 aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct tttttcttgc
    11461 tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc tcctaatatt
    11521 ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc ttcttttaga
    11581 tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt caaataagac
    11641 gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt gattttattc
    11701 aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc tgcttctgtt
    11761 tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc taagcattct
    11821 tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt ggcatcttta
    11881 gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc ttttgaaaaa
    11941 ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa agacatattc
    12001 gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt ctcagcatct
    12061 tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa ctttgcagca
    12121 tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa agattgattc
    12181 tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa ggcaattcca
    12241 aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc ccaagaactg
    12301 atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata acaaagtgct
    12361 ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg acgcaaaata
    12421 ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata ttcatcagca
    12481 ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc tgccatggag
    12541 ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg tactgttgct
    12601 gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt gcggttatag
    12661 atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata tacttctaat
    12721 gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc gttagttttt
    12781 ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac caatttgtat
    12841 ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg ctttcactta
    12901 tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt acttgtcgtt
    12961 ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt tttatataaa
    13021 aaga
//
LOCUS       DACDHJ010000057        12959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDHJ010000057 DACDHJ010000000
VERSION     DACDHJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.059"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IV223_001275"
     CDS             133..3660
                     /locus_tag="IV223_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8711574.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IV223_001276"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IV223_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8711575.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IV223_001277"
     CDS             7448..7789
                     /locus_tag="IV223_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8711576.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IV223_001278"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IV223_001278"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8711577.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IV223_001279"
     CDS             9146..11566
                     /locus_tag="IV223_001279"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8711578.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV223_001280"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV223_001280"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8711579.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4404 a         2249 c         2741 g         3565 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatctt
//
LOCUS       DACDHJ010000058        12901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDHJ010000058 DACDHJ010000000
VERSION     DACDHJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.060"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            141..479
                     /locus_tag="IV223_001281"
     CDS             141..479
                     /locus_tag="IV223_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711580.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(684..923)
                     /locus_tag="IV223_001282"
     CDS             complement(684..923)
                     /locus_tag="IV223_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8711581.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(899..1246)
                     /locus_tag="IV223_001283"
     CDS             complement(899..1246)
                     /locus_tag="IV223_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8711582.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(1978..2559)
                     /locus_tag="IV223_001284"
     CDS             complement(1978..2559)
                     /locus_tag="IV223_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8711583.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2742..3368)
                     /locus_tag="IV223_001285"
     CDS             complement(2742..3368)
                     /locus_tag="IV223_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8711584.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3390..4721)
                     /locus_tag="IV223_001286"
     CDS             complement(3390..4721)
                     /locus_tag="IV223_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8711585.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4876..5277)
                     /locus_tag="IV223_001287"
     CDS             complement(4876..5277)
                     /locus_tag="IV223_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8711586.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5363..5569)
                     /locus_tag="IV223_001288"
     CDS             complement(5363..5569)
                     /locus_tag="IV223_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711587.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5664..7166)
                     /locus_tag="IV223_001289"
     CDS             complement(5664..7166)
                     /locus_tag="IV223_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8711588.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7184..7639)
                     /gene="argR"
                     /locus_tag="IV223_001290"
     CDS             complement(7184..7639)
                     /gene="argR"
                     /locus_tag="IV223_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8711589.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7737..8354)
                     /locus_tag="IV223_001291"
     CDS             complement(7737..8354)
                     /locus_tag="IV223_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711590.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8575..9948
                     /locus_tag="IV223_001292"
     CDS             8575..9948
                     /locus_tag="IV223_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8711591.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10023..11135
                     /locus_tag="IV223_001293"
     CDS             10023..11135
                     /locus_tag="IV223_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8711592.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11207..12844)
                     /locus_tag="IV223_001294"
     CDS             complement(11207..12844)
                     /locus_tag="IV223_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8711593.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3494 a         2739 c         2294 g         4374 t
ORIGIN      
        1 taactaaaaa aataatccaa gaaacattga acctatggat ccaaaagcta cccaaatact
       61 atagctaacc ccccgctacc ttcaattttg cagataaaac gctttgggag aaaatttcaa
      121 agacaacagg ccatcctatc atgcccgatt ataaacaaaa agctggctat tccttttgct
      181 ctgacaaatt tcaattggga aagttcacga aactagcagg tgaagaagtt tcaactgttc
      241 ggatcaaaga atgcccgctt caaatagaaa cattggttca acgaatttca actagacaag
      301 actttgctat tgtagaacgt gagatcgtag ctatttttgt agaagaaggg attttacatg
      361 atccaactca tatcaatgtg gataaatggc aacctcttat ctataaattc agagaatata
      421 caactgccaa ccagtcgtta actttgaatt ttagattcca agaattcaaa gagtcataat
      481 gataaacatc gttaggtata aataaaaaag actcgcagca cgagtaaaaa acaaatagaa
      541 gctatacatt agaaaggtcg actgtgaccc tatagatgca gtatatgcag cttatgagca
      601 tttcttctgt tctctttgtt tttgggatct gtgctgaagt ctttttcgtt ttttgccgct
      661 ctttctttga aaaatcgatt atgttatacc ttctccatta ctaattcagg catctgctga
      721 gtaatgattt ttcgcgcaaa agaaatatct gttacccaag gttcgatttg ttcttcagtc
      781 aagacaagcg gcattcggtc atggattggt gcgactgtgc ggttaggttc agtagtcatt
      841 atgattgatt ctgccatttt ttcatcctga tggctacgat aaaaaccgcc aatatacact
      901 attttttcct tatttgaaaa cataaatttg tctttttctt cattccactc attgggaaaa
      961 cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact gtctctgcac
     1021 gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc atgatttccg
     1081 gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac ggaaaaactt
     1141 ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct cgataataat
     1201 tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta atccctccca
     1261 atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg ctttcaattt
     1321 ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt attctcctgc
     1381 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt tttgaaattg
     1441 ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa
     1501 gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa
     1561 tttttcttaa tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca
     1621 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagcag ctatttttat
     1681 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcca ctaaacgatg
     1741 ttcaatcagc taaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
     1801 ccgtttgttt ctctaatact caggtcaaag cgctgatttc acaacctcta atacacggtg
     1861 ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaatttg agaagcaatt
     1921 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct ctaattatta
     1981 aagaaccttt tgcgaagtta acaatctttc tttgatcaat tgaaaattct gcctaagtag
     2041 tgatagatcc tgtgtgactt tcaacaataa gatacttccc tggatttcag caatcaacgc
     2101 tttggcttga cggtctgctt cttcatcgga cagtttttct tcagaaagtg ccaggatatt
     2161 ttgtttcgta tacttgaccc attgattgac taattctagt agcaacagac ggtattcttc
     2221 atccactgta ctcaactcaa taatcaaatt acctacagga cagccatgat aagtgatctg
     2281 tgtctcatga tcagcgaaaa tccagtccaa catttcttct aatgcttctt ctggctgttt
     2341 tccagcattt aagatgtctt cgaacaattc tgtctgccat acgtccatat gcgcatggat
     2401 caccgctaga caaacttctt tttttgaaga aaagtaataa taaaattgac cttttcctgt
     2461 atcagaagct gctagaatat ctcggattga cgtttcatga tagccttttt tataaataag
     2521 atctttggtc gcttccaaga tcttttcctt tgttttcaat gatattctcc tttttcattt
     2581 ctcatagtcc tatattatac agtaaattca tcattttttc ttcttaatcg tttaaaaaag
     2641 actgtgccaa attatctgac acagccctca ctataaaact gttctcatat agctagatgt
     2701 tcattgattc aagaactgcg aggacaaaag caattcctat cttatgatag ttgctctaat
     2761 acttcctctg ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc
     2821 attgcttctt ggccaggagc accaattgca tctgtcgcta ccacaacatt gaatccactc
     2881 tctaccaatt cacgcatatg ggaatcgcta cacaagtttc cgttcattcc accaagaatt
     2941 actgtgtcga ttccattttt tctcaattgg agcgccaaat cattgctttc tggtccaaag
     3001 attttatgag gagagctgat gatcgtattt tcatccaaat aaggtttcaa ttcttcgata
     3061 aaatcggctc ctgaaccttc ttccactgat tcatattgac tttttctagc aaacatctgc
     3121 tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa
     3181 aaataatgag gagaaataaa gagtttgtat cctttttctt ttgcttgttt taccatttca
     3241 atcatatttt cgatcgtatt gtatttttct aagatatctt ttgtcaaacc aaagccaccg
     3301 cctgctggtt tcaagaactc gacttgcggg tcagtgagaa ctactgctgt atttttatta
     3361 ttgaacatgg tggattctcc attcgttatt tattttaata catcatttag agcttcactc
     3421 attgtcggat gagaatagat ttgatctctc aataacgtat aaggaagttt tccattcata
     3481 gctagcgaaa tcaagttgat gacttcatga gaatcttccg catagatact tgcccctaga
     3541 atcaagtcag tttcagggtc gactaaaact ttgaatacac ctttaggatc ttctaatact
     3601 tgtgctttag ggacaccgct tgtcatatac ttgaataatt tgtaattgac gccttgtgct
     3661 ttggcttgtt tttctgttaa acctacattg gataaagtcg gagtgataaa tacactgtaa
     3721 ggcacagctt tccggttatt cgttgttcgt ttattctcgc ccttcaactg atccataatg
     3781 attcgataat catctagaga gatataagtg aactgcaatc cgcctttgac gtctccaatc
     3841 gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca cagcgattgc tccgcgatct
     3901 gttagtttaa catctgtatt ttctaatcca agtgatgctg tacttggttt tcttccagtt
     3961 gcagccaaaa cttttgacgc ttggattgtt acttcttggc catcttctgt aaaggtgacg
     4021 gcagctgttg attcttggtc tgctacttga tctacagata cacctagatg gaattcgatt
     4081 cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc acgtggaaga
     4141 aattcagaat gggcatcaag aacgacaact ttagatccgt attcattgaa cattgatgca
     4201 aattccaagc caatatatcc agccccgatg atcactaatt tttccggtaa ttgttcctga
     4261 tccatcgctt gcgtagaatc aatcaagtag cgacttgtat tcaatccttt gattggtaaa
     4321 atgattggtt cagaaccagt attgatgaaa atccgttcgc cttcaacaat cactttttca
     4381 ccattattcg tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc gtccatcact
     4441 gtcccagttg cctcatcgct gatcatatga taatttttgg cgcgaagcat gcccgtcaat
     4501 ttttctttcc gagcaacagc ctctgtaaag tcgattcctc tttctccatt gaaaatcaat
     4561 gatttagaag gaatacagcc gatgttgata catgttccgc catacatacg tgttgacttt
     4621 tcaacgacta aaaccgattc accttttgaa gctaacgcct tcgctaaagt cttaccgccc
     4681 tttccaaaac cgatcacaat attttgatat ttttccatca tttttccctc gcttcttttt
     4741 tacctaccga ttggtacaaa aatatgataa ccgataattg tctattctcc aaatcatctg
     4801 ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt cacgttttta gtctggttcg
     4861 atttccttta ttcttctaaa caatcgttac ttctacatcg atatttcctc tagttgcttt
     4921 ggagtaaggg cagaattcat gcgcatcttc tgcaattttt tgggcttctt cttcgctgat
     4981 gccatcgata tgcacttcta atactacacc gatctgatag ccgccatcat ctaaattata
     5041 aagagagaca cgtgctttta ctgtggactc tgctgtcatt cctgcttctt tgatcatatg
     5101 ttgtaccgcc ccattgaaac aagaagcata acctgctgca aataattgtt ctggatttgt
     5161 tccttctgca tggattcctg gaggagtgat tttcatatgc atcgagccat ttggtgcctc
     5221 gacttctcca ttgcgcccgc catggttgat cattgctgtt tcgtatgctt ttttcattgt
     5281 actgctcctt tcgattgacc atttgctact tggctataat tatagtgtat gacaagtaag
     5341 gcgctttcta caaaaattct gcttattcga ataccagaat gccgtcttct actatttttc
     5401 caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat atttgttcca aaataaatag
     5461 caatcttttc tacataatct tttatatgat tgattttaag atacatatat accagaaagt
     5521 tgaccccata aaccaatact gttaaaaaaa taaccatatt cgttaccatt ttttcctcct
     5581 tttgctggtc tccctaccag cactcgattt aaccatacca ctttttttgc cgtccaagca
     5641 actagccttt caaaaagact ggtttattct gctgttaatg attcaagatc agaaaaatgt
     5701 ccgaataatt ttgctaattt tactaacgcc aacttgttct ttccgaattc atcattttct
     5761 atctgtaata ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg
     5821 tatggctctt ctagatcata tcgaatacct tcttcatttt ccgggtcgaa gttcatccct
     5881 ttttcttctt ccaacagtcg cttcatttct tctaatatct tgtttccttg ttcgcgtggt
     5941 tcatcgctga acaattttaa tcggatttct tgtacttcta cgggctgttt caactctttg
     6001 atttgttcat ctaatgtgat accttttctt tttaattctg gcaaaagcat caggatcttt
     6061 gcgatgacat atgcaccatc atccagaaaa tagttttcct tgaatgcagc gtgtccgctt
     6121 gtttcgatgg ctagctgtgt atctttccca agacggttaa gttctaatgc tttattgatt
     6181 acatttcgat agccggaaat ataacgaact tggatgcctc ctttttcttc aatgaatctt
     6241 tgtagatgag aagaagtagg ggaattcgtc acgattcttg ttcctggatg ctcttttaat
     6301 acgattgtac ttaatactgc gatcaaatta tttcgattga tcactgttcc agaagcactg
     6361 acaactgcag aacgatccac atctgtatcg aaaatgatcc caagatctgc ttcatttgct
     6421 aaaacagcct gcttgatact ttccatcgct tctttattat caggatttgg gatatgatta
     6481 ggaaatcggc catctggttc caaaaattgg gaacctgttg aatccgcacc taagctattc
     6541 aatacttttt cagcaaagaa tccaccagca ccatttccgg catcaacaat gattttccaa
     6601 ccatttagtg gtttgctttc tagtgttcca atcccctttt taatttttgt gactaaatct
     6661 tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc gctttttatt
     6721 ttttgctgct gtgataggat aaaagcaata tcttcttttt ctgctcctcc ttctgaggta
     6781 aagaatttta ttccattata ataataggga agatggctag cagtcagcat aaccgcagca
     6841 tcgcacgaga actgttcaaa ttgagtggac ataaacatag caggcgtagt agctaattcg
     6901 aaatcgatga catgtatccc ttgttctttc agactttgga tcaacgcctc tttcagtgct
     6961 ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc attttttatt tggatcggtt
     7021 gatttcttct ttttctttat ccagtttgct agtccccagc cgatcttttt taccatgtct
     7081 gtcgtcaagt ttacttcata tccttcaact ggtaatgcga ttcctcgaat atccgatcca
     7141 ttttgcagcg ctttcatatc tttcatataa aagtcctcct catttattcc ctcatttttt
     7201 ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata atgaatgtat
     7261 ctacaccggc catcgttccg acaacttcag ggtaagccac ttcatctagc caattcgtta
     7321 ccacatcggc accatttcgt tcagtcaata cgataaccat gaactgaaca gactggatac
     7381 gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc atttaaagaa acagatggct
     7441 ggttaaataa tgtatagcgt actattttgt tttcattttg actttttact aacttcattt
     7501 ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc gcctgcttca tgcagccgcg
     7561 caagcagctc ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg atcagtgctt
     7621 gtcgctgttt ctttttcata ttagacacct ctcaatgttg attatagcaa aagataagta
     7681 atatttcttc atattttggt attttttact tttatttttt gttttcattc aggttgctaa
     7741 cctttttcta ttttaacacg caaaatcgtc ttcatttttt caggtgctga ttttcttgga
     7801 tcgctgatgt aaatctcttt gtgttcttta gaagctcttt catagccttc ttttgcgatg
     7861 aatgcttcta atttctcaaa cgttgctggt tcttcggcat agggcccgac gtgtaagatt
     7921 tgggcacaaa gaccttcttc catctttttg aatactactt gttctttcaa gtcttccgga
     7981 agtttgctag aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt
     8041 cggatcatca attgataaga aaaatgttct tttttcatca ctggttcatg caaatacttt
     8101 tcttgcagtc cccattgtcc ttccagtgga tagacagtat acatctgata atcaggaatc
     8161 agccaatttt ttttaggcga catgcgtatt gcatagctga ctgcatatag acaggctact
     8221 cttcgtttaa agtcttcctg attcgggtcg ccgatccctc tgatgctaaa gaatgatttt
     8281 ttaggaacct ctagtatttg cgggcttttt ttcatactat agctcttgtc tttacgccat
     8341 tcaatttttt ccatttttct tcctccgcga aattccattt agttatttat tatcatacct
     8401 ctttttgagt gacttggcta atcgtgttga cactattttt cagtttgtgc tagaataagt
     8461 gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc gagagagagg cggtttctat
     8521 ttttataata gaaatcactc tcttttttta cgaaattttt tagaggtgat gtaaatgaca
     8581 aaacaaaaac agctgctttt agtacctgca gtaattgcaa cagggatgat gtcttttgcg
     8641 ggcgtattga tcgagacggc catgaatgta actttcccaa ctcttatcag acagtttgat
     8701 ttaacgactg cacaagtcca atgggtaaca acgatttatc ttttgatgat ttcgatcatt
     8761 gttccgctgt ctacttattt gaatcgcaat tttagtctca aaaaactctt tttagcttca
     8821 aatctgcttt tcttggcagg tataatgatc gatttcttct ctcctacctt catggtccta
     8881 ttgtttggtc gattgctcca aggtgcagcg acaggtatcg ctcttccgtt gatgttccat
     8941 attatcttga catttgcccc cctcaatcga cgaggcgcga tgatgggtgt cggtactttg
     9001 acaacggcca ttgctccagc aattggcccc acttatggag gcattatgac ttcacatttt
     9061 acatggaatc atatcttcct gtttcttgtg cccgttttga ttctttcttt gattatcggc
     9121 tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt atttggattg gataagcgtt
     9181 ttaggaattt gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt gggaacagtt
     9241 tccagctgga ttgctttaat catcggcggt attggctggt tcctttttta ctatcgaagt
     9301 aaaaaagtgg agcatccact gatccgactg tctattcttt caaataaaac attccgcttg
     9361 tttttgattg gcttcttagt ttatcagttt ttattacttg gtgtatcttt tgtcttgccg
     9421 aatttccttc agatcgttca aggaagtagt ccttttgtcg ctggattagc gatgcttccc
     9481 ggcgcagcgg ttggtgcttt gctttctcct ttttccggga aaatgctgga tcaatatggt
     9541 ccgaagaagc caatttttgc tgggctgatt ctttctttga ttggttggct agcattattc
     9601 ctactcatag gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc
     9661 ggtatcggat tatcttacag caatatgatg accaccggaa tgaatgcact gaatgaagaa
     9721 ctacaaggag acgggaacgc aatgttcaat accttgcagc agttttctgg cgctgtggct
     9781 acttcattag tagcggtcat tatcaattac gtccagcatc atacttccca taactatgaa
     9841 gtgtctacta cattaggatc aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta
     9901 agctttgctc gattcgccta ttatctgttc tttgctaaaa aagcgtagat tccatatacg
     9961 gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt
    10021 ttatggaaaa aattgccatt atcggtggcg ggatcattgg tatgacccta gccagttact
    10081 tagatacatc aaaattcaat gttgctttat ttgatgaagg gacaggacag gcaactaaag
    10141 ctagcgctgg gattatctcc ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc
    10201 tggctaaaga cggcgctgct tttttcccta aacttataaa agatttccaa cttgatcaaa
    10261 cgatttacgc acaatcaggg acacttttgc ttcgacctga aaaagattta gctgaattgg
    10321 ctgttcttgc cgagaaacga aaagaagaag cgccagaaat tggaaacatc gaatggcttt
    10381 cacctgaaca gacatctcaa gcacttccgt tattgaaacc agcagtttca ctgcgtatca
    10441 gtggtggtgg tcgtctggat ggaaaagcat atttagaaca tcttggtcag cgtttagcac
    10501 aaaaacaaat ggagcagatc caggcaaaag tcactttgca taagaagagc gattcttggc
    10561 tcatcaaggg accgtttgga gaatacacag ctgatcgtgt aattttgaca cctggcccgg
    10621 cacttaagcc tttattacaa gaattaggct atgctgcaga tatacgtccg caaaaagggc
    10681 agctcttagc atttcagaca tcttataaga tgagtcaaga ctggcctgtt gctatgcttg
    10741 acggagaagc tgatttcatt cctttcatgg atggaaagat ccttttaggt gctacccatg
    10801 aaaacgatca aggctgggat ttggctccta ctagcgaagc ttataaggga ttgactaaga
    10861 gtacagaaca gttcttgact gatccagatc agataaaaaa ctggccatac gagttccgtg
    10921 taggtacccg tgcttatacg tcagattttt ccccattttt cggcgtactg ccagatgatc
    10981 caagcttact ggctgctagc ggtctaggct cttctggctt gaccacaggt ccttatatcg
    11041 gctatctatt agccaacttc ttaaatactg gtcattggcc tacggaagat tatcaaaagg
    11101 acatctccac ctatattacg aaaaatccat catagaaaaa cagctttttc aagtaaggat
    11161 cccgagcgat attgcgcatg gattacttct tgaaaaagct gttattttag ttcaatttct
    11221 gaatcaaatt ctccaaactt tcttttgctt catctggcaa gagatctttt cctatctgag
    11281 cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac cagattttta taggacgtat
    11341 cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg gatgtcttca aatggaccaa
    11401 acgacttgaa ttctgccgtt tgttgcacga ttctttcaac gattgacaat gtcgtttcag
    11461 gtttgatttt tttatcatcg aaatatccag tattcaggac atagcaggct gtttgctgtt
    11521 tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag aggatagaca cggaaaggat
    11581 tagcaaatgg ttcaatgact agctcatcta gctgtctttt tgtagtgata ttttccgcgt
    11641 ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc taaaactggg tcattgatct
    11701 tgataattgg cggaagcgaa tcatctttca tgatccaaaa aacagcatcg atcttttcgc
    11761 taagatgatc gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt
    11821 ttcgtatatc tcctgttact aggacttttt ctcctttact tgtacatgtc acgcccacat
    11881 tctgacaagt aaggaaagaa gaaatggccg gatcatcaga cggatagtct tgtgttttat
    11941 caaagtaaga aggttccaag gcagtagcaa aaccattttt tcgagaaata acgaaagcat
    12001 cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta atcgttgatt
    12061 ttccagaacc ggacaaccca aatacagcca tcgtataggt ctggtcatta ctagcatatt
    12121 gtttcatccc accgtggcag gcaataaagc cattgcgatt tcccaatgcc caagcaagcg
    12181 tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc tagcacaacc gctgtattat
    12241 ggagagcatc aaacaaaatg agtccatttg gaaatgtttc atgtgtccat tcgggatcac
    12301 aaaagatata aagatctccc tcatcaaagg aaacagactt ttgataagtc tctaagatct
    12361 tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa attgttctca aatccttctg
    12421 gaatcagcaa atgactttgc acagaaaatt cttcagatag gccaacgatt acttttgtat
    12481 ggtaaaactg gcgcttgcga ctttgataca cagcttctct taaaattttc tcgtatttgc
    12541 tttgttcctt gtctaagtcc tcaaagatcc gccgagcact tgctgttctt cccacaatgc
    12601 ttccatcatt gaatactagt gtcctaacat cagctggtaa atctagttct tcagcatgtg
    12661 agattgttaa atctgtgatg atcgttcctg gactcccttt tgctagttga taagctgtct
    12721 tcctatcaaa aacttcatga acattattac cgtaaaatgt cgtttctaca atcgatcgaa
    12781 tactggataa cagtggattt ccacttgtga tttcgtttaa ttcgagactc tcttttgtac
    12841 tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc tttattatac attattcgag
    12901 t
//
LOCUS       DACDHJ010000059        12883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDHJ010000059 DACDHJ010000000
VERSION     DACDHJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.061"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            372..941
                     /locus_tag="IV223_001295"
     CDS             372..941
                     /locus_tag="IV223_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8711594.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            928..1788
                     /locus_tag="IV223_001296"
     CDS             928..1788
                     /locus_tag="IV223_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8711595.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1978..2682
                     /locus_tag="IV223_001297"
     CDS             1978..2682
                     /locus_tag="IV223_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8711596.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2687..4522
                     /gene="walK"
                     /locus_tag="IV223_001298"
     CDS             2687..4522
                     /gene="walK"
                     /locus_tag="IV223_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8711597.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4519..5835
                     /locus_tag="IV223_001299"
     CDS             4519..5835
                     /locus_tag="IV223_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711598.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5836..6705
                     /locus_tag="IV223_001300"
     CDS             5836..6705
                     /locus_tag="IV223_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711599.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6760..7569
                     /locus_tag="IV223_001301"
     CDS             6760..7569
                     /locus_tag="IV223_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8711600.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7672..8301)
                     /locus_tag="IV223_001302"
     CDS             complement(7672..8301)
                     /locus_tag="IV223_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8711601.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8471..9763
                     /locus_tag="IV223_001303"
     CDS             8471..9763
                     /locus_tag="IV223_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8711602.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9839..10501)
                     /locus_tag="IV223_001304"
     CDS             complement(9839..10501)
                     /locus_tag="IV223_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8711603.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10726..11010
                     /locus_tag="IV223_001305"
     CDS             10726..11010
                     /locus_tag="IV223_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8711604.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11061..12686
                     /gene="groL"
                     /locus_tag="IV223_001306"
     CDS             11061..12686
                     /gene="groL"
                     /locus_tag="IV223_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8711605.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4293 a         2211 c         2785 g         3594 t
ORIGIN      
        1 gtacttaata atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca
       61 gaaaaatctt tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt
      121 atataagcat accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat
      181 tttttactcc tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa
      241 gaaggcttag ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt
      301 aaagcaacta gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag
      361 gagcggttat gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa
      421 cattccgtct gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca
      481 gaacgttagt tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta
      541 cctcttctat ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa
      601 gatataacaa agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga
      661 ctattcctga aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag
      721 cctataatct attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac
      781 aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa
      841 gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa
      901 ccgaatattt aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat
      961 tcaggtacgg atgtgccaga agaattgata aaaaaatacg agatgtatat gatcccattg
     1021 aaaattattt ataaagatca ggtatataca gataaaattg acattacctc tgaagagatc
     1081 tatcaacgtt tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca
     1141 gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc
     1201 agtggattga gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg
     1261 gaagcatatg tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa
     1321 gcagcaaaat ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag
     1381 ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt
     1441 ggacggatcg gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc
     1501 tcatgtaatg aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt
     1561 ttggataaaa cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta
     1621 gctgttgccc atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga
     1681 gagttcccac aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac
     1741 actggtccgg gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg
     1801 aaacactgag gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata
     1861 aagtattttg cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa
     1921 agcagaaata cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg
     1981 aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta
     2041 gccaaagaag gctatgatgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt
     2101 gctgaggtag aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta
     2161 gaagttgctc gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa
     2221 gattctgaaa ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag
     2281 ccattttcca accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg
     2341 gctgcgaagg aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc
     2401 catccagaag cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa
     2461 ttcgaactac tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta
     2521 ttacaaacgg tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta
     2581 cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt
     2641 cgtggtgtcg gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt
     2701 ccgctttttt cgatcagtca attttaagat cgctattacc tttattctga ttttattgat
     2761 ctcgatcgaa attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac
     2821 atttattaaa gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt
     2881 gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga
     2941 aaacagtgcg actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg
     3001 aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat
     3061 caatgatttc acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat
     3121 caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa
     3181 aagtaatatc gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc
     3241 ctcactaatt gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac
     3301 acagccgatc ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg
     3361 gaaagtcaaa gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt
     3421 agctgaacgt attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag
     3481 cgtgctatcc catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac
     3541 gatcaatgat atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc
     3601 gattttgaca cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc
     3661 aaacgaaatg gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg
     3721 agtagacttt tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt
     3781 gcatgatgtg acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt
     3841 ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga
     3901 aggtgcgtgg aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac
     3961 agaccgaatg attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa
     4021 tacccagctt caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg
     4081 gtttgatatg atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca
     4141 aagagatctt tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat
     4201 gaataacgcg atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac
     4261 gcataataac gtggttttaa gtatcaccga tcaaggattg gggattccta aaaaagatct
     4321 gaaccgagtg ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg
     4381 gacaggactt ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg
     4441 ggcagagagc aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta
     4501 tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg
     4561 atggttgcgt taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt
     4621 tcaatcaacg tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact
     4681 tccgaagtct ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc
     4741 aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag
     4801 ctttctgaag tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct
     4861 ggttttgagt tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat
     4921 ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata
     4981 caattcgatc aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat
     5041 gaagcaaata tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca
     5101 aaatggacag aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat
     5161 tcgatcaaac tgaaaaagta tagttatatc ctgtcacagc aaccctatac actttttaga
     5221 aatgcttttt tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt
     5281 ttttacggtg ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt
     5341 ggagagctgc cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt
     5401 agcaaattag gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc
     5461 gattatcgga tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt
     5521 ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg
     5581 aatacgattc aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa
     5641 atcatccagc agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt
     5701 ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg
     5761 tatgtgaaat ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga
     5821 ttggaggcga attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta
     5881 gggttgaatg tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc
     5941 attcgctcta atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac
     6001 acggggaaga tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat
     6061 cttagcactt cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt
     6121 gtgtttttag acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac
     6181 gtgatcgatc cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat
     6241 attttattcg gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca
     6301 ctagaaatcc aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa
     6361 atcgttcttt ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg
     6421 catttatctg atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca
     6481 atcaatacat tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag
     6541 ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat
     6601 gtagcgatcg aaacaagcga taaagtggtc caaattgaga gtgtcaacgc tttcagcaat
     6661 acgatcgtga caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat
     6721 gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct
     6781 tttaaaatca gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa
     6841 aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct
     6901 caaattgatc ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat
     6961 catatacatg gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag
     7021 cagacttggg aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta
     7081 tttgagatgg ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc
     7141 catgacgcag cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg
     7201 ttgacggaca ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt
     7261 tatttgatgg aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat
     7321 ttaaaacagc gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc
     7381 atgactgatg tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa
     7441 aacaatatga aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt
     7501 ggcgttggac atgattttga aatctatgat acagatccag atgaagcaac tgagctattt
     7561 tcgatttaaa cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt
     7621 tggttttatc ttctattttt atttttttag catattaaaa aaataaccgt attatcgact
     7681 gactacactg atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat
     7741 cgcgtaatct gccatgctga tcgtactgtc gccagcagcg tttctttgta aataatcatc
     7801 acttaacaaa taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat
     7861 ataagtccaa tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa
     7921 agctgttgga tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc
     7981 tgtatctaag aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc
     8041 aagcatctca atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc
     8101 gttgaaagca tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa
     8161 atccttttgt ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt
     8221 cacgtctaaa tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc
     8281 tccaatgagt gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt
     8341 tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg
     8401 ttcgtgatca ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag
     8461 gagataagaa atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt
     8521 gaatcttaga cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat
     8581 tgttggctcg ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat
     8641 agacaaaaca ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat
     8701 agccttttgg atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg
     8761 cttaccagtc ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac
     8821 tcagatagat actgtctgga caggtgctga agggatacta ggagcagtta caactgggct
     8881 catcatcgga tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga
     8941 gaagatacct gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt
     9001 cacttttacg cttatcggtt cagcggtttt tcaacagttg ttcagaatga ctgttgttca
     9061 aggagtatgg acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt
     9121 gcttttatta ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat
     9181 ggtcaatggg ttggttagtc ccgtcttgca agcgaattca ttagcgaatg cagaaatctt
     9241 agcctcagga caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct
     9301 cgatcagttc atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac
     9361 ggtttttgcg aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt
     9421 tttcaacatc aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc
     9481 tgttcctttt attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt
     9541 tggattcgtt cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc
     9601 gggtttctta gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag
     9661 ttttttcatt tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag
     9721 acagacaaaa aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct
     9781 gtcgctttag attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt
     9841 atactagccc tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt
     9901 gtgtgatcat cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac
     9961 ctaggaaaaa gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc
    10021 tgatcactgc tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc
    10081 ggaatacgaa ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg
    10141 cttccatgat catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca
    10201 atacagattc taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca
    10261 atccataaat aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg
    10321 gttcacgttg cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg
    10381 cagttgctgt gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg
    10441 gtgagataaa aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca
    10501 tgtaattcct ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc
    10561 aattatatca taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa
    10621 agcttgcaaa aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt
    10681 aagagtgcta acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta
    10741 ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt
    10801 ttagcttctg ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc
    10861 cgtttgttag aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg
    10921 ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca
    10981 ggaaaagata tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata
    11041 atcaatgagg tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag
    11101 ctatgttacg cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag
    11161 gccgaaatgt cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga
    11221 cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat
    11281 cagaagtagc atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc
    11341 tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag
    11401 gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct
    11461 caactgttgt tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg
    11521 ataaagttgg acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca
    11581 caatcgaaga atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg
    11641 atcgtggtta cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag
    11701 aaaacccata tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat
    11761 tgttagaaca aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg
    11821 gtgaagcatt gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg
    11881 taaaagctcc tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga
    11941 caggtggtac agtaattact gacgatctag gtttagaatt gaaagatgta acaatcgaaa
    12001 acttaggaaa tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt
    12061 ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa
    12121 caacttctga ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg
    12181 tagctgttgt taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca
    12241 ttgaagatgc attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg
    12301 gtacagctct agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg
    12361 caacaggtat caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa
    12421 acgctggtta tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg
    12481 gtttcaatgc tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa
    12541 ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga
    12601 cagaagcagt cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc
    12661 catcaatgat gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt
    12721 agtgaaagac agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga
    12781 aattcacgga aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc
    12841 ttctgcttcc gctgtttata cgtgtttaga gcgtgagaca aaa
//
LOCUS       DACDHJ010000060        12678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDHJ010000060 DACDHJ010000000
VERSION     DACDHJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.062"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            158..1003
                     /locus_tag="IV223_001307"
     CDS             158..1003
                     /locus_tag="IV223_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8711606.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1194..2381
                     /locus_tag="IV223_001308"
     CDS             1194..2381
                     /locus_tag="IV223_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8711607.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2387..2743
                     /locus_tag="IV223_001309"
     CDS             2387..2743
                     /locus_tag="IV223_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8711608.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2745..3083
                     /locus_tag="IV223_001310"
     CDS             2745..3083
                     /locus_tag="IV223_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8711609.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            3478..4512
                     /locus_tag="IV223_001311"
     CDS             3478..4512
                     /locus_tag="IV223_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8711610.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4505..5191
                     /locus_tag="IV223_001312"
     CDS             4505..5191
                     /locus_tag="IV223_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711611.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5215..6063
                     /locus_tag="IV223_001313"
     CDS             5215..6063
                     /locus_tag="IV223_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8711612.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6260..7030
                     /gene="sufC"
                     /locus_tag="IV223_001314"
     CDS             6260..7030
                     /gene="sufC"
                     /locus_tag="IV223_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017368843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8711613.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEA"
     gene            7043..8329
                     /gene="sufD"
                     /locus_tag="IV223_001315"
     CDS             7043..8329
                     /gene="sufD"
                     /locus_tag="IV223_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8711614.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8326..9561
                     /locus_tag="IV223_001316"
     CDS             8326..9561
                     /locus_tag="IV223_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8711615.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9548..10018
                     /locus_tag="IV223_001317"
     CDS             9548..10018
                     /locus_tag="IV223_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8711616.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10023..11414
                     /gene="sufB"
                     /locus_tag="IV223_001318"
     CDS             10023..11414
                     /gene="sufB"
                     /locus_tag="IV223_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8711617.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11512..12654)
                     /locus_tag="IV223_001319"
     CDS             complement(11512..12654)
                     /locus_tag="IV223_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8711618.1"
                     /translation="MATFFCYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4062 a         2378 c         2765 g         3473 t
ORIGIN      
        1 atgattacga gcaagcagac agctgattgt cttcatctaa gttactagtt ggatcagcaa
       61 ctgttttgtt gtacatttct attatagatg gttttttgta tatttaaatc aaatagtttt
      121 tatagtaatt atcataaaaa gttatggaag tggtgccatg tttaagttat taacgacttt
      181 tcgagcagta tatgaaacaa gaaatttttc cagagcagca gaagtactgt ttttatctca
      241 accagcagtc tcgaatcaaa tcaaacagtt ggagcgggaa ttgaatatcg atctttttca
      301 acgtaacgga agaaaagaga tgcttccaac taagcaagcc gacttattat atgaacggac
      361 actggttttg ttagaagaat gggaagaaac tcagcaaaaa ttgctcaatg aacaaaatga
      421 agaagaagta tgtcggatcg ttgcttccca cacctttgct gtctatcttt tgcctcagtt
      481 gatgaaacat ctgatcaaaa ggtttcctaa agtgcatttt gagatcgaaa tcacgaattc
      541 tcatgaatca ttagaacaag tagcgaaaca tgaatccgat tttggtttta tcgaaaagcc
      601 attagagact tccggcgtac aaagatactc tttgatggaa gaccagctag tcatcgctgg
      661 ggacccaaaa agcaaattgt ggcttgtaag agaagcaaac tctggtgttt ttcattacac
      721 gcagcgttat tttgaagaga tgaatatcca agataagaag atgatcgtga aaagtaacga
      781 tgtaattgtt gcgttgctga aggaaggaat cggtcgctcg attttgtcta agcgctctgt
      841 tccagaaaat gtgccaatga ctccgttgaa tttccatcga tatttttatt tcatccaacg
      901 aaccaatctg gtttctgatc agctgctaga gattgccgaa gagatccttc gatacaatga
      961 aagcacagaa acttctcaca gcgaggagaa tctcagctgg tagataattg catgtatcaa
     1021 aaaaagttct atgatacact aaagataagc agtaggcttt tgtactgcat ttgctcaagc
     1081 aactgcacaa acggaaagtt aatttttccc aaatgtctat ggaaagactt tccattatct
     1141 aactggaccg ctataataat cagcagtatg gtttttaaga aaagggatga aacatggaaa
     1201 aaaaattaaa agcttcgaac gataatcgta ttgattatgg cgtgatttta ccagtctttc
     1261 tgttatgttt gattggtctg ctctcattat atgtagcact tagccatgat ccgaacaatc
     1321 cagcagtctt gaaaggcgta gggatgcaag ttgtctggta tttagttgga gcggcagcaa
     1381 tcgttgtcat catgcatttg aattccaaat ggatctggaa actgactcct tacctttatg
     1441 gtttggggtt agtggtaatg ggactcttgc tcaaattcta tgatcagaat ttagctgctt
     1501 caacagggtc taaaaactgg tttcgattcg gtgcgtttac tttccagcca gcagagttga
     1561 tgaagattgc gtacatcttg atgatggcac tgatcgtaac taaacataat acgcaagtaa
     1621 aagaacggac gatgaaatcg gacttttggc tgattgggaa gctcttgatc gtgacaatac
     1681 cggttttagc attgatcatg gcccaagatg actttggaac gatgcttgtc tttcttgcga
     1741 tctttggcgg gattttcctg atgtctggaa tctcttggcg catcatcgca cctgtagtaa
     1801 tcttggcagt agtcgttgga gcaggaacga tcttcttggt tacgactgaa ggcggaagag
     1861 acttgcttta taaagttgga ttcaaatctt atcagtttgc acgtattgat tcatggctgg
     1921 accctttcca tgatacgtca ggaatgagtt atcagccggc ccaaggactt ttggcaattg
     1981 gtaccggtgg cttgtttggt aaaggattca acgtaagcaa tatctatgtg cctgttcgtg
     2041 agtccgatat gatttttacc gttatcggtg aaaacttcgg ctttatcggt ggtgcattcg
     2101 tcattttctt gtattttatt ttaatctatc gtatgattcg ggtgtgtttc gatacgaata
     2161 atgaatttta cgcctatatc gcaagcggtt tgatcatgat gctgctgttc catgtctttg
     2221 agaatattgg agcaaatatc ggattacttc ctttaacagg gatcccattg cctttcatta
     2281 gtcagggagg atcctcgatt ttagggaaca tgatcggtat cgggttgata ttatcgatga
     2341 gataccaaaa tgaagcacct gcaagatctt caaggaggta agaaaattgt acacctttta
     2401 ctggtacccg aaatgttcaa catgcaaaaa agccaaagcg tggttagacc agcatggcgt
     2461 tagttatcaa accgttgata tgatcaaaga agtaccgaag gcctcaacga ttgagacttg
     2521 gctagaaacg agtcaagtac caatccgtcg cctgttcaac acaagcggga tgaaatatcg
     2581 ggaacttgga ctgaaagatc aagtaggcga gtttacggct tcaaaagcga gccaagtctt
     2641 atcgacagat ggtatgctga tcaagcgacc actgatctta cgagaggacc agcttgttgc
     2701 aattggattc aatgaaaaaa tttatgaagg agaactgata taaaatggca ggcaagcatt
     2761 tgaagaaaaa ggacaacctt tggatcttat ttaatggaaa agaatactgt gttggtttga
     2821 caaatgaagc gcaagaagaa cttggagaca tcacgtttgc caatctgcca aaagcaggac
     2881 aatcgctaaa aaaagatgaa cctctgatcg aagttgaagc ggaaaaagcg gtcaatgaat
     2941 ttgccagccc gctaacgggt acagtttctt ctgttaatga taaaatcggt gaagatatca
     3001 atatcttgaa tgattcagat gaaatgaatg cgtggatctt aagtttcaaa gatgttgatc
     3061 caaaagaatt tgacgaatta taaaatcaga aaatcacttg aaaaaagttg acactgattc
     3121 ttccagttgc tataatcata agcaatccta tatgaaacga gaaagctttg aaaagaagag
     3181 tacatgtcac ggacgtttga agagagccct gtttgctgaa aatgggtaac gaaaatgaga
     3241 tggaagatgg tcttagagca gagtaagcga gcgtaagcaa acttactacg ggagtgcccg
     3301 ttacagcgcg acagtatacg gaaagaagct aagtccaagt actggaagag gctgttattg
     3361 cgaagtaacg gtaaatcaag gtggtaacac ggaagttcaa gctttcgtcc ttttgcttaa
     3421 ggcaagaggg cgaaggcttt tttgtttgaa aaatcataag agaaagatgt gaatggcatg
     3481 tcattaatcc aactaacgaa tgtcaaaaag acattcaatg ggaaaaacgg aaacatccaa
     3541 gccgtcaatg acgtttcact aaacgttgaa aaaggggata tttatggaat cgtcggctat
     3601 tctggcgcag gaaaaagtac attagtccgt ctcctgaatg gacttgagct tcccacgagc
     3661 ggcgaagtga tcgtaaacga tcaagatatt acaaaattaa aaaacaaaga attacgtaca
     3721 ttccgaaaga aaatcggtat gatcttccag catttcaacc tgttatggtc aagaactgta
     3781 cttgaaaata tccagctgcc gctggaatta gctggcgtac caaagagcaa acgtaaagaa
     3841 cgggcagaag aactgttgcg tttagtcggg ttagaaggac gcggacaagc atatccttca
     3901 caactttccg gcggacagaa acaacgcgtc ggtatcgcac gagcattggc aaatgatcct
     3961 gagatcctgc tttgtgatga ggctactagc gcattggacc cgcaaacaac agaagaagta
     4021 ttagacttat tgttagcgat caataagaaa ttgaatttga ccatcgtctt gatcacacat
     4081 gaaatgaatg tgatccgcaa aatctgcaac aaagtcgctg tgatggagtt ggggaaagtc
     4141 gtagaagaag gagacgtatt gactgtcttc agacaaccga aaaaagaagt aaccaaacgc
     4201 tttgtacaac aagacatcga accagaagct gattcgacag aactattagc tgaactcatc
     4261 aaagaaaatc caagcggccg tttggcaact ttgaccttta gcgaaagaaa tgccaacgag
     4321 ccagtcattt cccaagcgat tcgacgctat aacatcgatg tcaacgttgt gtatggaaag
     4381 atcaaacaag tgaaagaagg ttcctttggt tcattgactg tcttgatgac tggtgaaacc
     4441 aaagaattag atcaagcaga agtgttcatc aaagaacaag gagtaggaat tgaggtgatc
     4501 catcgtggat aaaagctttt cagaaacata tctagatttt aaacaaatca acccggaaac
     4561 gatgaatcag gctatccttg atacactata tatgacatta atctcaatgg tcttagtgac
     4621 gatcatcgga ttagttttgg gattgatcct ttactctatt ggacgcaaaa acaaaccgta
     4681 tgctcgaatc atttatggaa tcgtatcgat catcagtaat attttccggt caacgccatt
     4741 tatgatcctg atggttttga tcattccatt tactaaagca atcgtaggca ctatgctagg
     4801 tgcgcaagca gccattccag ctcttgtttt atccgcagca ccattttatg cccgtctagt
     4861 agagattgct tttcgtgaag tcagccctgg tgtactggaa gcagcagatg caatgggagc
     4921 aagttactgg gaaatcatct ggaaggtctt gatacctgaa agtattcccg cattgatttc
     4981 tggattaaca gttacaacga tctcgatgat cggatttact gcgatggccg gcgcgatcgg
     5041 cgcaggtgga cttggaggtc ttgcttggca ggaaggctac cagcgtggta atctaacagt
     5101 gacattcgtt gcgactttaa ttattttact aatcgtattt atcgttcaag gaatcggtga
     5161 tttcttaact aagaaaacag acaaaagata ataggatata gggggatgta ggatatgaaa
     5221 aagaaaattt ttggatttgc agcagcgctt ttgctgactg taggattagc agcatgcggt
     5281 aacaacagcg acagcaaaga ttcagcaaat aaagcagacg acacgacatt gaaagtcggc
     5341 gcttcaccga caccgcacgc agagattttg gaacatgtga aaccattatt gaaagatgaa
     5401 ggaatcgatc ttgaaatcgt caaattcgat gactatgtat taccgaacca atctttggaa
     5461 gaaggcgaca ttgatgccaa ttacttccag catattcctt atttgaacaa agaaatcaaa
     5521 gaaaaaggat atgactttgt caatgccggt gctgtccaca tcgaaccaat gggcttgtac
     5581 tctaaaaaag tcaaagatgt ttccgaatta aaagatggtg caacaatcat tacttcaaat
     5641 tctgaatcag actggggacg catcatcaca attctacaag atgctggctt agtcaaagtc
     5701 aaagatggag tagatctgga aacagcaaca ttcgatgata ttgaagaaaa ccctaagaac
     5761 ttgaagttca accatacgat cgacccatca cttttagctt ctacgtacca aaatgatgaa
     5821 ggtgacctag ttgcaatcaa cgcaaacttc gcttacggcg cagggttgaa tccattagaa
     5881 gatgccgtat tgcttgaaaa ggacaactct ccttacgtga atattattgc gactcgtaaa
     5941 ggtgatgaag acagtgacaa aatcaaaaaa ctgattgaag tgcttcaccg caaagaagtc
     6001 caagaatgga tcacagacaa atggggcggc tctgttaaac cagtaaaggc tgatgcaaaa
     6061 taaggtatgg aacaaaaaag atgagaaata agtctgtaat ggcttatttc tcatcttttt
     6121 tatacctatt ctaatttaga aatcaaaatt gtttaataat aaataaaaga tatttgattt
     6181 tagtaaggaa ggtcattgcg cttcattctc aattagtcta aaatgagtga gaacgaaaga
     6241 gaatttgggg gtataatgca tgtctgtctt agaaataaaa aatctacatg tgtcgatcga
     6301 ggaaaaagaa atccttaaag gcgtaaacct aacaatgaaa accggagaaa tccatgccat
     6361 catgggacct aacggcacag gaaaatctac cttatctgcc gctatcatgg gcaatccaaa
     6421 ctatgaagta acagaaggcg aaattttgtt tgacgggaaa aatgttttag acttggaagt
     6481 agatgaacgt gcgcgacttg gcttattttt agctatgcaa tatccaagtg agataccagg
     6541 tatcacgaat gcggaattca tgcgtgcagc gatcaatgcg aaacgtgatg aaaacgacaa
     6601 aatttctgtt atggcatttt tgaaaaagtt agatgaaaaa atggctttac taaatatgcc
     6661 agaagaaatg gctgaacgtt atctgaacga aggtttttct ggcggagaaa agaaacggaa
     6721 tgagatcctg caattgttga tgttagaacc tacatttgca attttagacg aaatcgattc
     6781 aggtctggat atcgatgctt tgaaagtcgt tgctaaagga gtaaatgaaa tgcgcggaga
     6841 caatttcggt gcattgatca tcacccacta tcaacgatta ttgaactata tcacacctga
     6901 tgtcgttcac atcatgatgg aaggccgagt agtcatgaca gggaatgctg acctagctaa
     6961 acgtctggaa gcagaaggct acgctggtat cagtaaagaa ttaggaatcg actataaaga
     7021 agaagcgtaa gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt
     7081 aaatgatttt tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc
     7141 actagcgaaa gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg
     7201 gtcgttattt gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt
     7261 tgatgcaatg aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca
     7321 actgcctgtg gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat
     7381 cgaatatcca gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga
     7441 ccaattgact gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa
     7501 aaatgtcgtg atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga
     7561 gcacttcttc aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga
     7621 acgtttccag acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt
     7681 catcgctaaa gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat
     7741 tacttcttat atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat
     7801 tggtgtcatg aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg
     7861 tgcacacgca gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga
     7921 tacacgagta accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat
     7981 ccgagaaaaa ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg
     8041 tgcagatgcc caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc
     8101 gaatcctatt cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg
     8161 acgagttgat ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc
     8221 agagcgctta gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca
     8281 agttcggaaa gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa
     8341 aagctacgca atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat
     8401 ctggataatg cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat
     8461 tacgaacacg ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc
     8521 catgattatg aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca
     8581 gaagtgctgt ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc
     8641 gaccgctata tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat
     8701 gttatcccgt ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta
     8761 acagaagaag gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa
     8821 atcgtctcca ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta
     8881 gctgaacttg tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg
     8941 cacatggcca ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa
     9001 atgtgcggac caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg
     9061 gaacctgttg aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg
     9121 aaagaactac cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta
     9181 ggccatgcaa tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa
     9241 gaactagccg cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt
     9301 ccacaagacc cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc
     9361 catgatgtcg cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt
     9421 gcgcaaccgc tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc
     9481 tacaacacaa cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt
     9541 ttccaacatg ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc
     9601 ttcccatccg catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa
     9661 ccccacatgt ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa
     9721 tatcgccttt gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga
     9781 cgccgtcatc ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct
     9841 tgtccaagga aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg
     9901 tgtagctaaa tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa
     9961 agcaatggaa aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga
    10021 acatgggcgt accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg
    10081 ttttcagtac aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag
    10141 gcgaaccaga atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc
    10201 caatgcaaac atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc
    10261 aaaaaccaag tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata
    10321 catttgaaaa gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac
    10381 aatatgaatc agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg
    10441 tctttactga cactgactct gctttgaaag aataccctga attattcaaa gagtactttt
    10501 caaaacttgt accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag
    10561 gtgggacctt tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact
    10621 tccggatcaa tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag
    10681 gagcaagcat ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc
    10741 atgccgccat cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc
    10801 aaaactggtc agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg
    10861 caaccgttga atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg
    10921 tttacttaga tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa
    10981 accaaatcca agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta
    11041 tcgtctctaa atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct
    11101 ttgccaaaaa cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg
    11161 aatggtcgaa atccgatacg atcccattca atgagatcca caacagccaa gtctctttag
    11221 aacacgaagc caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag
    11281 gcctgaccga atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca
    11341 aagaacttcc aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag
    11401 ggtctgtggg ttaataacta tttcattgct cgaaacgttg atattaaagc gtttcgagcg
    11461 tttttttatt tgtattttaa tggttggcta cgtttttggc tacgtttgga attattcgcc
    11521 cataaatttt gcaaatcggt caccagtttt cttaacagat tgtggtgtga catgagcata
    11581 gatattcatg gtagttttaa tatctttatg acctaatcgt tcctgtactt cttttatcga
    11641 cgcaccactt tcaaacaata agctacagtg ggtatgccta aatccatgtg gagtaatttg
    11701 aggctccatc ggtgtctttt tgtaaatcca gtctaaccaa tcgtttacta cttgggggta
    11761 gtacaacttg tttgtgtttg tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc
    11821 atataagatc attattttca tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa
    11881 atcatcaagc tgaatcgttc ttgatgaatt tttagtttta ggtacttgta gaactacttg
    11941 attaaactcg tcttgagcga tagttttacc aattgttatt gttttattga aatagtctat
    12001 atcttcccat tgaagcgcca atgcttcgct tttacgcatt cctgtataag ctaataaacg
    12061 gaaaaaagtg tacagtttaa ctgaattatt ttctttagta ttctgtaaaa acagttgtaa
    12121 ttgatctttc gtgtaaaact tcaagggttg ttcttcttca atttttcttg gaagcaatgt
    12181 tttagacata ggattatcct tacagacttc cattgctact ccaaatttaa atatttgagc
    12241 agttgttttt ctcaagaaac catattgttt taatgttttg tgccactcat tcacctgttt
    12301 ttgacaataa ctaacactta ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg
    12361 gttcttgcaa aaacgttgag caacggcaac agaagacggt tttactcttg ttctgtagtt
    12421 atcaatccat aattcataca actcttgaaa agttgtaaca tttattttaa actcttgttt
    12481 ttctttaaag tctacaagta gtctattaag tgctgtttgc gcctcagctt ttgacttgaa
    12541 tcttctacgc tttgtacgta tatcttttcc agtcttggga tcttttccta aataagtttc
    12601 aaatccccaa tatttttcgc cattttgtaa tttataacaa aaaaatgttg ccatttgatt
    12661 ctctccttac ttcatgat
//
LOCUS       DACDHJ010000061        12606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDHJ010000061 DACDHJ010000000
VERSION     DACDHJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.063"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            116..1999
                     /locus_tag="IV223_001320"
     CDS             116..1999
                     /locus_tag="IV223_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8711619.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2074..2358)
                     /locus_tag="IV223_001321"
     CDS             complement(2074..2358)
                     /locus_tag="IV223_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8711620.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2348..3082)
                     /locus_tag="IV223_001322"
     CDS             complement(2348..3082)
                     /locus_tag="IV223_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8711621.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3211..3834
                     /locus_tag="IV223_001323"
     CDS             3211..3834
                     /locus_tag="IV223_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8711622.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4026..5147
                     /locus_tag="IV223_001324"
     CDS             4026..5147
                     /locus_tag="IV223_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8711623.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5154..8282
                     /locus_tag="IV223_001325"
     CDS             5154..8282
                     /locus_tag="IV223_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8711624.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8301..11495
                     /locus_tag="IV223_001326"
     CDS             8301..11495
                     /locus_tag="IV223_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8711625.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11641..12348)
                     /locus_tag="IV223_001327"
     CDS             complement(11641..12348)
                     /locus_tag="IV223_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8711626.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4297 a         2218 c         2658 g         3433 t
ORIGIN      
        1 aatttatggg atgatagaaa aaaatagtag agaaaggaca cgattctact tgctattttc
       61 acttaatacc cgtatattac gaatgaaaaa agtttatgaa aagttggaat attgaatgga
      121 aaaacaaaaa aggttaaaaa acagccgcta catcactggg tttgatgggc tccgttcatt
      181 agctgtggtg ggtgttatct tctatcacct tctgccaact agtatgaaag gcggctattt
      241 gggcgttccg atttttttcg tcgtatctgg ttatttgatc acggatctgc tgcggcagga
      301 atgggaacaa aatggaaaaa tcaatatttg gcaattttat gtcagaagaa tgaagagact
      361 ctatccggca cttgttttta tgcttgtgac agcttcggct tatatcactt tgtttcagcg
      421 agggctgtta aacaatcttc gaggagttgt ggtcagcagc ttactgtatg tcaacaactg
      481 gtggcaaatc aataacggtt tatcttattt cgatcgattt gctaatgagt caccgtttac
      541 gcatatctgg tctttagcag tagaaggtca aaactatttg gtttggccga tcttgtttgt
      601 attgttgatg gtatttgttc gtaaaagaaa atggatcttt tatactgtgt taggtacatc
      661 cttcctctcc gccatcttga tgatgatctt gtatacacca ggaggcgatc caacccgtgt
      721 ttattacggt accgacactc gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt
      781 ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat
      841 cgctggagga atctcgcttt tggcactgat tctttgcttc ttctttttag atgatcatta
      901 caatttcatc tattacggag gaatgctcct agtgagtctg ttatgcgtag tgctagttgc
      961 agtgacagca caccccggag caagtttgaa tcgatggctg acaaatccag tatttacttg
     1021 gattggcaaa cgaagctatg gcatttatct gtatcagttt ccagttatga ttttttatga
     1081 agcaaaggtg actgatattg ccgatcatgt tttgttgcat actttagtgg aaatcacatt
     1141 gattctaatc atcagtgaac tttcttatcg atatgtggag cgtccgctag cacgttttga
     1201 ttatggtcgt acattccaag tgatgaaagg atggttcagc aaaccgatca tcagtaagag
     1261 caaaccatgg gttgttccag gcacattact tgtagcaatt gcactcgttg gtttcgttac
     1321 ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg caggcaaaaa tcgctgaaag
     1381 taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac accactagtt cggttgacca
     1441 agcgatcctt gataagtacg agttgtccaa ggcacaaggg aataaggcac aaaaattaga
     1501 actaacggca tttggtgatt cagttatgtt ggatgctgca acggatctac aagaacttta
     1561 tccaaaagta gtggtagatg gagatgtcgg acgacaatta tatgccagtg taccgtacat
     1621 tgaaaaatta aaggaacaag atttgttgaa agacactgtt ctgatcggac taggaacgaa
     1681 tggctcattc tcggaagctc agtttgatag tgtgatggat gcaatcggtg atcgaaaagt
     1741 atactgggtc aatgtccgag taccgacgaa gcgctggcaa aatgacgtga atgcgatgct
     1801 tgaacagatg acgaaaaaat acgacaatat gacgctgatt gattggtaca gctacagtaa
     1861 tgaccatagt gactggtttt atgatgatca agtccatcca aatcctgaag ggatgacaca
     1921 ttacattcac ttagtcagcg aagccttgct aggaggaagt tcaagtacga acagcagtga
     1981 gacaaacgtg acagaatgat agctacaaaa aacttcaggc aaaaacattt gtttttgcct
     2041 gaagtttttt tatatttacc gtgtttttaa cattcatata gattcaggaa gcaaaacggt
     2101 tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg agttttttga atgcagcttc
     2161 tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca tggatcatct taacaggtct
     2221 tcttttttga agacgagtat atttagcacc agtaccattg ttatgttcct ctaatcgtct
     2281 agaaagatcg gttgtatatc caccataaaa tgtctgatct ttacagaata atacgtaaaa
     2341 ataatgttca tttgccatag agcatctcac gaatctctgc agtgtattct ccatccatat
     2401 tgtaagtgat caatggtgga gcgactttga atccgtctaa tttgccttct ttgattcctt
     2461 caactaacaa aatatttgcc tcttttccag gttttgggta aacaaattgg atccttttag
     2521 gtgcaattcg atagtcttcc atgacctgaa tgatttctaa gaaccgatca ggacgatgga
     2581 ccatagtaaa gcgtccattt gtttttagta gcttacttga aatatcgatc acttcttcta
     2641 acgtcgtatg aatctcatga cgggcgattg ccaggtgttt attagggttc gttttatttg
     2701 ttggcagccc tttgaaataa ggtggattac agacgactaa gtcacaagaa ttatgtcgaa
     2761 cgacagataa gctgtctttc agatcgatgg tgtggacagt catttgttca tttaaatggt
     2821 taagcgcgat actacgtctt gccatgtctg ctagacgagg ttgaagttcg atagcgtcga
     2881 tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt
     2941 caacaatctt ccctttttta ggtacacggg aaaagtgagc aagcagcaca gcatctaaag
     3001 aaaaagaaaa cactttggaa ctttggataa tcttgatatc ttctgcgtat aattggtcga
     3061 ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga aaaaaatagt cgtctgtatt
     3121 atactatttt tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac
     3181 tatgaccata agaagggaaa ggaagagact atgttttatc gatttatgcg cggggtagtt
     3241 cgagtactat tagccttagt taatggaaat gcccgctatg agaataaaga agttttaccg
     3301 caagatgaga attacatact agttgctccc catcgtactt ggtgggaccc attatatctg
     3361 gcggtagctg cagcacccaa ggaattcagc tttatggcaa aagaagaact gttcaaaaat
     3421 ccagttctgc gatttatatt aactcgatcc aatgcttttc cagttaaaag agaaaatcca
     3481 ggaccaagcg tcattaaaac acctgtaaag attttgaaat cgacaaattt gagtttgatc
     3541 atgtttccca gtgggacaag acatgctaca gaacttaaag gcggaatggc attgatttcc
     3601 agaatgtcta aagcgaaaat cgtgccagct gtatatcaag gtccgttgac acttggggat
     3661 ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac caatcgactt atcagatatc
     3721 aagaaaacgg ataaagaagg tatggcggag atcgaacgac gaacacaagc agcttttgac
     3781 aaattggaca aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat
     3841 aagtttatgc gtgggcggtc ttttgaatct actgtcctgt tcagttcaga ctttttcaat
     3901 gaaaacgagg gagagacgtc aagtctgccc tctttttgcg ttccaatatg taaaatagaa
     3961 gacaggtgtc atgtaatgga tcaaagtgtt ataataatta gaaggacatt ttttgaggtg
     4021 attgaatgcg ttttttacat acagccgatt ggcatatcgg aaaaaaacta caaggttatg
     4081 acttgattga agaccagcgt catgtactat cttctatact agatatcgcg aaagaagaaa
     4141 aagtagaggc aatcgtgatt gccggagact tatatgatag gagtgtccca gctgttgatg
     4201 ccgtagaatt atacaatgaa ttgatggtcg attggaattt aaaggaaaag atcccgattt
     4261 ttgctatatc aggaaatcac gatagcagta cgagactttc agcgggtacg ccatggttca
     4321 gttattcgaa ctattttttg cgaacacagc tagcagaagc atttgaacca gtcgagttta
     4381 aaaacgtaca atttttcttt cttccttact ttgaaccaat tgcagcgaga ttatattttg
     4441 aagatgacaa tatccgaacg atcaagcaag cggtagccaa ggtgatagat aaaatgcagg
     4501 agctatttga ttcagaaaaa aaacaagtat tgattagcca tttttttgta atgggaagtg
     4561 taaaatctga ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc cctggtgaac
     4621 tacttacacc gtttgattat gtagcactag gacatttgca taatcaagct gctttgagac
     4681 aggataatgc tcgctatagc ggttccccat tgaaattctc tttatctgag atcaaccaaa
     4741 aaaaaggcgt atggatcgtt gacctgacaa caggcctgga gttgaatttt agggagatcg
     4801 aacccctgca tgatattgaa gaaataacag cgagtttcaa agaattgctg gatcctgcct
     4861 tttatcagtc cgtcaatcgg gagaattaca tctatatcta tctcactgat agaacgatca
     4921 tcccgaatat gatgaaccag cttcggcaaa tatatcctcg tattttgggg attgaacggg
     4981 caaacggtcg agcagaacaa acaaagcgca aacgaaaagt actagaaatg aaagatcctc
     5041 aaaaattagc agaagcattt tttagtgaaa tgacggaaga gcagctaact gcaaaccaag
     5101 aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac
     5161 cgaaaaaatt acaacttaaa aattttggtc cttttatcaa tgagaccgta gatttttcac
     5221 gtttgacaga agcaccgctt tttttgatca gcgggaagac aggagctggc aaaacgacga
     5281 tttttgatgg gatcaccttc gcactttttg gtgaaacttc cggaagactt cgctctggaa
     5341 aagagatgcg ttctttgttt gctacaccag acgaagaaac aagcgtgact ttttcttttg
     5401 aacatcagca gatgagctat gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa
     5461 aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt gaccattttt gatgggaaag
     5521 gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga tcagttgatc agagaactga
     5581 tgaatctcga tgcgaaacag ttttctcaaa tcgttttact accccaagga gaattccgta
     5641 attttttggt ttcatcaagc agtgataaag agactgtttt acgtcattta ttcggtactc
     5701 aatttttcca acagttcaat gagcgattaa aagaaaaagc aaaacaacag caacaaaaat
     5761 tggatcattt agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag
     5821 aggaaccagc agcagggttt gaagccgttt tagcccaatg gacaaaccat caggcaatac
     5881 tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc
     5941 aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga
     6001 tattaataaa caagcaaaag gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac
     6061 gatggattgg gtattatgaa tttacgcaac gattagtcga accaatccag cgcctacaag
     6121 aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca aagaaactag
     6181 cgatgactac gcagaattat gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac
     6241 aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg
     6301 tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt aacaatttca ttggataaag
     6361 aagaattgaa tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa
     6421 ttcttcaaga aaatttactg gatcaggaaa aactgcaaga agatcgtctt gcttttggac
     6481 agttaaaata tctagatgaa aagcagcaag aaatagaaaa acaaaaagca aaagacactg
     6541 ggtcgctcgc acagataaga gaagaactca aaaaagcaac tgaagaaata acaaaaaata
     6601 gtcagctatt gaaagaacag acagaacaag cagaacagct gaaaagccaa tgggcaaagc
     6661 agcaaattgc tcgtcttcag ctttttttgc gtccagggga gccatgtctt gtttgtggat
     6721 caacggaaca ccccatacaa caattaaaac acgaacagcc aaataaggaa gatatcttaa
     6781 aagtagaaca acaactggca gaagctgagt ctgctgtaga aagaactcag caaatcattg
     6841 ccaaaagtga agcacatgaa aataatcttc aacaacaatt atcagattta gaacaaaaaa
     6901 tcatggcaac gaatatacaa ttggctgaac aagaagaaca cttgcaacag gctttgaaaa
     6961 aggcatttcc tgagataaca gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat
     7021 ctttagatag acgaaaaagc aagatggcca atgcaagaat agagctggaa gaaataagac
     7081 aacaagcaga aaagctcgaa caacaattag ctgtctacaa acagcagcag acagaaaaaa
     7141 ataatcagct ccagcaactg caaggagaaa tcaaaagttt agaacaacaa ttgcaagata
     7201 cggatagtaa ctgtttagaa gaggagatac gacgacttga aaaacagtta gaagaagact
     7261 cgaatcaact agctgttgag cagaaagcag gagtgcaatt gttgcaagat cttcatacgc
     7321 ttcggacaca gcttgatctt caacatgaac aaatagaaaa aatagatgaa aaaatagaaa
     7381 aggaacaaga aagaatagct aaaaagttag aaagccagtc gtatttttca acaattgaag
     7441 aaatctgtgc atttgcacaa cagcaagcac aatataaaga aaaccaacgt acagttactt
     7501 cttatcatga agagcaatta gtgacagctg accggctgcg tcaattagaa aatatccagc
     7561 taactgagac attcccagat acagaagaga tcagccaaca gttagaagaa ataagacgta
     7621 tgatcgaaat aaagcaagaa aagatatacg gtttacaaga acaaaaaatt agtaatcaaa
     7681 aaatctacga tgaatgtctt tcgatttatc acacaagcca gacgaaatta gatgagctta
     7741 cgcaattaca gcagctttcg caaacattca acggagaaaa tcctaagaag actagtttgg
     7801 aacggtatgt gcttcaggtt tatcttcaag aagtgttaca agtagcaaat gatcatctcc
     7861 aacgtttaac gaaaaatcgt tatcaatttg agctagcgga taccattggc agttatcgag
     7921 ggaaaaccgg attagaaatc aatatctatg atgatcaggc aggtatgtct cgaagtgcgc
     7981 ataccctgtc tggcggtgag agttttattg cagcactttc tttagcatta gctcttgccg
     8041 atgtgatcca gacacaagct ggtggtgtag caatcgatgc attgtttatt gatgaaggat
     8101 ttgggtcatt agatgaagaa gctttggaaa tggcaatgga agctttagaa acgatcgaaa
     8161 gtgaaggaag aatgatcggc atcattagtc atgtaagaga attgaaagaa cgagtattac
     8221 aacaaataag aattgataca gaaggtagcg gacaaagcaa aatacgctat caacttggat
     8281 aaaaagggag attggtaact atgaaatgga gtattccaga aaaagtcatt gcacgtggac
     8341 gtcaatactt gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt
     8401 ggcatgcaga agtcttgggc agtgaattgt atatggttga tttggatggt acagcaaaag
     8461 agatagatta ctgccaatgt ccctattggg aggaacataa atattgtaag catacagttg
     8521 ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa
     8581 tgctagaacc ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc
     8641 taaaaacaca aaacaatcct caagcggtgc aacctttgat tattgaatac caagtagaaa
     8701 cgattgagac taatcagtat cacccagagc tttctatttt ggcactttac ttgcgtatcg
     8761 gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa catctatgat tttttacaag
     8821 cttacacaga aaaagaaact tacactgtta ataaacagta ccagtttcgt ctagataaac
     8881 gggcgtttca aaaagaagat caaaaaatac tcacgcatct tgcaggaagt gctcagacgc
     8941 atcaattatt aggtacgaac ggcttacaag tcaaaggaaa actagataaa agatatctgc
     9001 ttctcccagt agaacaagcc cgcttcctgt tagagcagat gaatcagact acacgtttgc
     9061 atctgcagat aaatgaacaa aacgatcaag gaatacaatt ttccgatcaa aacaaacctt
     9121 tatcattcaa agtggaaaaa tttggagaag attatttgct gcaagcattg aatgattttg
     9181 attttttctt tatgcattat cagtggggag ttatccaagg gacattttat tctctgacga
     9241 aatatcaaca aacggtttat caaacttgga aacaattgat tcgccgattg gagaaaccag
     9301 aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa agagattatc cctctgctgt
     9361 cagagatcgg cgacgtagct attgcccctg cagtagaaga ggaagtagca gatatccccg
     9421 tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg
     9481 tctatggcga agtcatctat tcgactgacc cgaagcatga ggtctcgaca gaacaggctt
     9541 ttcaggtgtt acgtgacaca gtagaagaga aacgagtact tgatctattc agaatgtatc
     9601 gctaccagaa aaatgaaact ggttatgaac gtccattgcc aagcggggaa gaactatatg
     9661 cctttttccg cacagaacta tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa
     9721 aattgcgaga gttgtattta gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga
     9781 ccggttcatg gctcgatatt cgattcgatg tgacagggat agaggagcaa gaaatcgatc
     9841 atgtcctgca aagcttgctg agaaatgatg ctttttatac attagaaaat ggtcaggttc
     9901 tttcttttga ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa ttacgggaat
     9961 cgatacgtac agaagaagga acgatccatg tacctaaaaa tcaaggattg atcattcaaa
    10021 atcaattgga aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg gtacaagatc
    10081 tcattcatcc agaaagatac caggcacagc ttcccaaagg attgaatgca acgatgagag
    10141 attatcaaaa gcaaggtttt cgatggttga agatgttagg tcattatcag tttggcggta
    10201 ttttagccga tgaaatggga cttgggaaaa cactacagac aattgccttt ttactttcgg
    10261 aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca
    10321 actggcaggc agaggtgaga aaattcgccc cttctttaag tatccaagta atcaatggga
    10381 ataaaaaaga acgtgaagaa ttactggcta aagacacaga tatccgagtg acttcttatg
    10441 ccagcttaag acaggatcta gctaattatc agtcgcaaaa aattgattat ttgattcttg
    10501 atgaggcaca aatggtcaaa aatagcagta cgaaaactgc acaagcattg agagaattag
    10561 ctgtacctca gcggtttgca ctgagtggga caccaatcga gaataattta gaagaattgt
    10621 ggtcattgtt tgcaacgatt atgccaggat ttttcccgac taaaacaaaa ttcagggaac
    10681 ttgccgcaga agaaattgcg caaatgattc gaccgtttat tttacggcga gataaacaaa
    10741 ctgtgttgaa agacttgccg gaaaaaacag agatgaatct atattctgca cttacagaag
    10801 aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg
    10861 attctgaagc attcaagaaa aaccggatcg ggatacttgc tggtctgaca agactgcgac
    10921 aaatttgctg cgatcctcgt ctgtttattg aagattacca aggcggttcc ggcaagctcg
    10981 aacaagtaaa agatctactg gtagctgcca aagagaacaa acgaagagtt ttgctgtttt
    11041 ctcaatttac agggatgctg actatcttgc aagaagagtt agccgagcta gggatctcga
    11101 cattttattt aagaggcagt acgaaaccac aagatcggtt aagtatggta gacgcattta
    11161 atgctggcga aaaagatgtt tttctgattt ccttaaaggc tgggggaact ggattgaatc
    11221 tcacgggagc agatacggtc atcttgtatg acctttggtg gaatcctgcc attgaagaac
    11281 aagctgctgg acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga
    11341 tctctgaagg aacgattgag gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt
    11401 tccaaaaagt gatccaaggt aatgaagaac agttgacgaa actgactgaa gaagacatcc
    11461 gcatgatttt aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat
    11521 gggtaagaaa gcctttctaa agaagcgatt gtttatttga tttaaaaaat agcaaggagg
    11581 ctgggattcc attttatccc agcttccttg ctatcatttt tttatttcta tttttaaagg
    11641 ttatacaaag attgtcttca ttgtctctaa cagctgatca aaagaagaaa tctcgatatc
    11701 gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt
    11761 agcacttttt gcacctagaa catcattgtc gtaattatcg ccaacgtata aggtctcttc
    11821 tggaagcata tgaaattctt tctcagctag ctgaaaaatc tctttttctg gtttttggaa
    11881 acctgttgct tgagaaatga gcatattgcg ggaaggaatc caattgttca attgaagttg
    11941 cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg ataattccta atgggacatt
    12001 cttttccttc aagaaatcga gggttttttt gacctcctca tgcaagcaga tattatcaag
    12061 ttcttcctcg taaatttttt gaaataagag gccatcttct tccgtaatat gagggtagtc
    12121 taaatctttt agtgattggc tgatccgatg tgctcgcata tattctagtg tccaatctcc
    12181 tgccattact tttgggaagt tttcatcact atgatggcgg aaccggatat atagtggatg
    12241 catatctgca tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa atggctgctg
    12301 ctgatcatac atcgtatcat caacatcaaa cacaactgat ttaatcaaaa ttttacacgc
    12361 ctttcggttt tcattgattt tttcacaaat ccttgactat tgtaacagaa aaaattgtct
    12421 aaaaaagctg ataaataaag gtttttctta ctatatttct atttccttga cagaataagg
    12481 tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac
    12541 aaattaatca ttatggtaga agtcgattga tttattttaa tgcaatctct gtggaatttt
    12601 cgaaag
//
LOCUS       DACDHJ010000062        12603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDHJ010000062 DACDHJ010000000
VERSION     DACDHJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.064"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            268..2913
                     /locus_tag="IV223_001328"
     CDS             268..2913
                     /locus_tag="IV223_001328"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8711627.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3223..4536
                     /locus_tag="IV223_001329"
     CDS             3223..4536
                     /locus_tag="IV223_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8711628.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4526..5179
                     /locus_tag="IV223_001330"
     CDS             4526..5179
                     /locus_tag="IV223_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8711629.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5234..5926
                     /locus_tag="IV223_001331"
     CDS             5234..5926
                     /locus_tag="IV223_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8711630.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6076..6276
                     /locus_tag="IV223_001332"
     CDS             6076..6276
                     /locus_tag="IV223_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8711631.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(6530..8209)
                     /locus_tag="IV223_001333"
     CDS             complement(6530..8209)
                     /locus_tag="IV223_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8711632.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(8211..8423)
                     /locus_tag="IV223_001334"
     CDS             complement(8211..8423)
                     /locus_tag="IV223_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8711633.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            8816..10546
                     /gene="efrA"
                     /locus_tag="IV223_001335"
     CDS             8816..10546
                     /gene="efrA"
                     /locus_tag="IV223_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8711634.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            10543..12303
                     /gene="efrB"
                     /locus_tag="IV223_001336"
     CDS             10543..12303
                     /gene="efrB"
                     /locus_tag="IV223_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8711635.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            12398..12595
                     /locus_tag="IV223_001337"
     CDS             12398..12595
                     /locus_tag="IV223_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711636.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         3978 a         2182 c         2741 g         3702 t
ORIGIN      
        1 tgaagcataa cgctgaacaa gaggagtaac tggcacattc attcttagag agagaactat
       61 ctgctgaaag gttctcaatg acaaccagtg aaggtagctt gggagtttct gtcttgaatg
      121 gagtagagat agacgagtcg tgatcgttaa ctcacttgag aggcaataat gattgattat
      181 tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa tacgaaagta ttttaggacg
      241 ttttttagtt taaaggagga aaacagcatg acggaagaaa ataacctatc aacaaaatat
      301 aatccgcaag aagttgaagc tggacgttat cagcattggt tagaacaaga tttgtttaaa
      361 ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa tcccgccccc aaatgttact
      421 ggaaaacttc atttagggca tgcttgggat acgaccttgc aagatatgct gattcgtcaa
      481 aagagaatgc aaggatatga tactttatgg ctgcctggta tggaccatgc aggaatcgcg
      541 acacaagcaa aagtagaagc gaagcttaga gagcaaggga tttctcgtta tgatctaggt
      601 cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag agtatgcgag ccacatccgc
      661 gaacagtggg ctaaaatggg cttatcttta gattacagta gggaacgttt tacattagat
      721 gatggattat ctgaagcagt acgtaaagtt tttgttacct tatacgaaaa aggtttgatt
      781 tatcgtggtg aatatatcat caactgggat cctcaagcac gaacagctct atctgatatc
      841 gaagttattc ataaagatat cgaaggcgcc ttttatcata tgagctatga actagcagat
      901 ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa cgatgttggg agatacggcg
      961 attgctgttc atccagaaga cgagcgttat caagcgctca tcggcaaaaa agtgattcta
     1021 cctttagtca ataaacaaat cccaatcatt gcagatgaat atgtagatat ggaattcggg
     1081 acaggtgtag taaaaatcac tccagcacat gatccaaacg actttgaggt aggtaatcgt
     1141 catgatcttc cacgcgtaaa tgttatggat gatgatggta cgatgaatga actagcagga
     1201 aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa
     1261 atcggtcgat tgattaaaat cgatacgatg atccacagtg tgggtcattc cgagcggaca
     1321 ggtgtcgttg ttgaacctcg gctgtctact caatggtttg taaaaatggc gccgttagcg
     1381 aaaaaagcca ttgataacca agaaacagat caagcagtgg aattcttccc tccaagattc
     1441 aatcaaacgt ttttacgttg gatggaaaat gtccatgatt gggtaatctc acgacaatta
     1501 tggtggggac atcaaattcc tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg
     1561 atggaagcac cagcggatag tgaaaactgg gtacaagatc ctgatgtttt agacacttgg
     1621 tttagttcag ctttgtggcc tttctcaaca atgggctggc cagatacgga aagcgaagat
     1681 tataagcgat acttccctac tagcacttta gtaaccggtt atgacatcat tttcttctgg
     1741 gtaagcagaa tgattttcca aagcttggaa tttactggcg aacgtccatt tgagaatgtc
     1801 ttgattcatg gattgattcg tgacgaacaa gggcggaaga tgagtaaatc attaggtaat
     1861 ggaatcgatc cgatggaagt aatcgaaaag tatggagcag atgctctccg ctggttctta
     1921 tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct atgaaaaaat ggatgcttct
     1981 tggaacttta tcaacaaaat ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga
     2041 atgaccgtag aagagatcga tttatctgga gaaaaatcag tggctgaccg gtggatcctt
     2101 actcgtttga atgaaacgat cgaacgagta actgagctat ttgatcgttt tgaatttggt
     2161 gaagcaggtc gacaactgta taactttatc tgggatgatt tttgtgactg gtacatcgaa
     2221 atgagtaaag aagtacttta tggagatgat gctgtttcaa aacaaaccac ccgtagcatc
     2281 ttagtccata cacttgatca gatcctacgt ttattgcatc caatcatgcc attcgttaca
     2341 gaagaaatat gggaacatat tcctcatcag ggaaattctc tagtagtggc agaatatcct
     2401 gttgtacgtc cagagtttga tgatgaaaca gcttctaaag ggatggaagt attgaaagaa
     2461 ttgattcgag ccgttcgaaa tattcgttca gaagtgaata caccattgtc taaaccaatt
     2521 acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat ttttagttga aaatacaagc
     2581 tatatcgaac gcttctgtaa tcccgaagag ttgacgatat caagtgagat cactgcgcca
     2641 gatctagcga tgtcagctgt tctgacaggt gcagaaatct tcttgccgtt agcaggattg
     2701 atcaatattg aagaagaaat caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa
     2761 gtaaaacgtg tccaaggaaa actttctaat gaacgttttg tttctaacgc gccggaagaa
     2821 gtagtagagg cagagcgtgc aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa
     2881 gaacgaatcg aaagtttgcg tactattcaa taagaataga aaaagagagt gtgaaatcag
     2941 ctagctatta ctaaaagcta gctgattgaa catcgttaca ggaaagagtt tgagcctcac
     3001 tcttagcttc gattcataag gagatgtgac ataaattttg tcgcatctct tttttagtct
     3061 actcttatta tgtaggagaa ggagggaggt ggatcatgta tcaagagtca ataaagtagt
     3121 tgataaaatc gttcctctct ctgtttcttt tctctttgtc atacttttat ttttcttatg
     3181 tgttctattt gttataataa aagagaaatg ggggaattgt tgatgacgat tgaagaagca
     3241 atagaatgga tccatagtcg attaccattt ggttcacgtc ctggccttga ccgagtcgaa
     3301 gctttattag aagtggtagg gcatccagag agaaaagtaa aaacgatcca tatcgctgga
     3361 acaaacggaa aaggttctac tgtagcttat ttgagaagcc tattagaaga ggctgggctg
     3421 acagtaggta catttacttc accttatatc gaaacgttca atgaacggat cgcaatcaat
     3481 ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa aaaaatatca gccactgatc
     3541 aaacaacttg atcaatttga agcagtagct ggtattactg aattcgagac attaacagca
     3601 atggcatttg attatttttt gatgaagaaa gtggacgtag cgattatcga agttggctta
     3661 ggcggcttat tggattctac caatgtagct gtaccaatgc tgacgggaat aacaacgatt
     3721 ggacttgatc ataccgaaat acttggagat acaattgaag aaatcgctgc acaaaaagct
     3781 ggaatcatca aacaaggcgt tccagtagta acaggaaata ttgccccaga agcttttact
     3841 gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat
     3901 aaaataaact atctgcatcc tgatgagatg tggggagaag tatttgaatt ttttaatgaa
     3961 ataggaaaga tccctcaatt aaccactccg cttttagggc aacatcaagc tgaaaatgca
     4021 ggagtagcca tcgaattgta ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa
     4081 aaagatgttt tgaagggatt gaagaaatct cgatggccag gaagaatgga gcgtttgagt
     4141 aacgagccat tagttgtttt agatggtgcc cataatacgc atgcaatgaa ccggcttgtt
     4201 gagaatttga aaaaagaatt caaaggatat caaatcaaca ttttgttttc cgccttacga
     4261 actaaaaatg ttagagaaat gctagaacga ttgcagcaag taccaaatgt ccatatttat
     4321 ttgacgacct ttgaacatcc aaaagccatc gaattaagtg aagtatcaga tttagcaagt
     4381 gagagagtat caatcgtttc attgtggcaa ttcggattag cagatatatt ggaaaagatg
     4441 acaaatgaag acgtattagt tgtgaccggt tcactttatt tcatttcaga agttcgccat
     4501 ttattaatac aattaggagg agatcatgaa gtataatgca gtgatttttg acatggatgg
     4561 tttgctgttc gacacagagt tagtgtatta cgaagccaca caagtagtag cagacaagat
     4621 gggttttcct tatgataaag acctgtattt aaaattctta ggtgtttcag atgaagaagt
     4681 atgggcgaat tatcatatga ttttctcgga atttggcaaa acaagtgtac aaagattcat
     4741 agatgattca taccaggaaa cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc
     4801 cggtgtgttg gaactcctct cttttttaga agaacatcag attcccaaag ttgtcgcttc
     4861 cagtaaccaa cgagcagtca ttgaactttt attgaagaag aacaatctgt ttgaacgatt
     4921 tgagacgatt gtttctgcag aaaatgttca acgagcaaaa ccagacccag aaattttctt
     4981 gttagctaat cagaagctag gtacagcgaa gaaagaaaca ttgattttag aagattcaca
     5041 aaatggtatt ttagcagctt ctgcggctga gatcccggta gtcatggtac cagatctgct
     5101 tccaccatcc caagaattga tcaaaaaaac agctggggtt gtttcttctt tacatgaaat
     5161 ccctgcattg ttaaaataaa aatccatctc ttttcgtatc ttattttaga taagatagaa
     5221 aggagatgtt tttttgggaa aacgattgat caaagaagta cctactagct ctttaccaag
     5281 ggaacgtatg gaaatttatg gcgcggaagc tttatcggat caggagcttt tggcaatctt
     5341 actgcgtacc ggtcaacacc cctatagtgt gatgtcgatt gctggaaatc tgttaaaaac
     5401 gtttggagga ctggcctctt tacgacaggc tactttgcat gagttagaag agattcgagg
     5461 gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc gaattaggtc gacgaattca
     5521 gacggatcat gaaagagcgg ctccaccggt tcgtaccagc tacgaattgg cccagcaatt
     5581 gattctggaa atgaaagatc acaaacaaga acatttggtt tgtatttatt tggacacgaa
     5641 gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg ctgaatcagt cagtcgctca
     5701 tcctagagaa attttccatt atgctgtacg ttattgtgca gcaaggatcg ttttagcgca
     5761 taatcatcct tctggtaatg ttattccatc gcagcaggat atgaatttta ccaaaaggat
     5821 acaaaagtgt ggagaaatga tggggatcac tgtactggac cacttgatca tcggacgcaa
     5881 acgatatttt agtttgagag aagaaggaat gatggaagag aagtaagttg ctttcttgca
     5941 aaaaaaatga agcaagtgta caattatatt gtaattttgt ttgggaatag aagttgaaac
     6001 atctgttgca cctctatcgt acacgagcaa tgttacaatt taaattagtg cttttggcac
     6061 gaggaggata ttattatgga aaacggaaca gtaaaatggt tcaacgctga aaaaggattt
     6121 ggatttatct cacgtgaaga tggaagcgac gtattcgttc acttctcagc tatccaaggt
     6181 gacggattca aaacattaga agaaggtcaa gcagtgactt tcgacgttga agaatcagac
     6241 cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt atctgtaaat caaattgatg
     6301 gtagacatca atttttacga gagaacaaag cgagtaacaa aaatacctgc cacaagagac
     6361 aatttctctt gtggcaggta tttttttgta tgaaaaagta aataaattta agaggctatg
     6421 aaagagccac cttgtatcaa ggaataagaa caaacaaaaa tggcttctca tgtttttcat
     6481 ttgttttctc ttatattcag gacaaggtgc tgatttcata acctcttatt tattcttcat
     6541 ctggtgtcat taccattggc aaaatcattg gatgtctttc tgtacgttcg aataagaaag
     6601 gctgaagcgc gccagtcatc gcttctttca atttatattc tgaacagtca ggtccattca
     6661 aacattctct taaagcgtgg aataagattc gctgagcttc attgatcatt tctcctgatt
     6721 ctcgcatgta gacaaatcca cgagagagaa tatctggacc agccaagatt tcttttttct
     6781 tgatatccac tgtcgcgaca gctaatacaa gaccttcttc tgaaagcagg tgacggtcac
     6841 gtaaaacgac attaccaata tcaccgatcc cgctaccatc gatatacaca tctccagcca
     6901 caaaatgccc agcaggacgc gcagaatcgc tagttaacgc taaaacatcg ccattttcca
     6961 tgatgaagca gttttctgca ggaacaccta catcttgtgc tagagaagca tgtactttca
     7021 acatgcggaa ttctccgtgg acaggaataa aatattttgg cttcatcaaa cgaagcatta
     7081 atttttgttc ttcttgtcca ccgtgaccgg aagtatggat gttattgatt tttccgtgaa
     7141 tcacttctgc accagcttca gacagcaaat tgatcaaatg attgacactt gttgtattac
     7201 caggaatagg tgaacttgaa aagatcactg tatcgtctgg ctggatttgg atttgacggt
     7261 gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc tccttgagaa cctgtacata
     7321 ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg tgcatctaca aatgtgccgt
     7381 ctggaacttt gatatatcct aaacgttgac cgttgacgat ggcattttcc atgcttcgtc
     7441 cgaaaacagc gattttacga ccagttgcca cagcggcatc tgctgcttgc tgcaaacgga
     7501 agatgttaga agcaaagctg gcaaaaatga tacgtccttc gatttttgaa aagatcttca
     7561 aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt tggaatctca gcatttgtac
     7621 tatcagataa aaggcacaag actccttctt ccccgatacg agccatttta tgcaagtttg
     7681 ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc agtcgctacg atatttccag
     7741 aaggtgtttt caccacgatc cctaatgcat ctgggatact atgggtcgtt ctgaaaaagc
     7801 tgacagaagt ctttctaaaa cggatgatcg tatcttcatt gatctcatgg agctctgcat
     7861 ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa cgctaatgcg agaggaccag
     7921 catagatagg aatatttgct tgtttcagca agtagggtat tccgccgatg tggtcttcgt
     7981 gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt ttgtatgatg tagctgtagt
     8041 cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg gaatttgatc ccggcatcaa
     8101 tcaaaatgat ttcatcttgg aattgtaccc catacgtatt tttcccgatt tcacctaatc
     8161 caccaatagc aaagacgcct gtctcgttat ttttgatgga aagtttcata ttaaaactcc
     8221 actaatgaga aatcagcatt ttctttttcg tactctagat gacttccttc tagaagttga
     8281 acgaattcaa tattgtatgg ggtattttct tccacacgtt ggcgagcgtc tacatcgctt
     8341 tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag ggttgcgtac ttttgtttct
     8401 tgataataaa ctttatagat cattgttttg tctcctttat ctgcgtaaag cagtgattta
     8461 gctagttgat ttttttagac aacacaaaac gaggggacaa gccacctggt ttttcatatg
     8521 ctttgactag aatactggat tcgagtcaaa gagacaagcc tcgtttttgc ttatctgttc
     8581 ggatattttt tcaaccgtac cttctgtata aaaaatatac aacaattaat tgttattcta
     8641 gcataaaaca gcaaataagt atagaaatca actagattga aagaaaaaat caatgtttat
     8701 catcccgcta taatcataat gactttttta tgagagaagt gttctattaa taggctcgtt
     8761 gtgctatcat ttttagtaat gaaaatgtaa caaaagatta aaaggagatg agaaagtgaa
     8821 actaatgtgg cattatacca tgcgttataa gaaattcttg tttttcaatt tcatatgcgt
     8881 atttggtttt attttgattg aattaggact tcctacgatt ttagcacgta tgatcgatgt
     8941 aggaatccgc aatgatgatt ttgattatgt gaagcaacaa gggatattta tgatcgttat
     9001 tactgtaatc ggtattattt tgaatatctt gttaggttat tttggttcga ggattacgac
     9061 taatatcgta gcagatattc gagatgattt gttcgaaaag atccaaagtt attcgcatca
     9121 ggaatacgaa aatctaggcg tatcttcatt gatcactcgt acaacaaacg atgcttatca
     9181 gatcatgttg ttcctgcaaa atattttacg tatcggtttt atggcaccaa tgatgttcat
     9241 tgtcagtttg tacatggtca tgcgtacaag tgtgacactc agtttgtatg tagtaggtgc
     9301 attaccttta ctattattgg cagtcgtagc gattgctaag gtgtctgaac cgttatctaa
     9361 aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg cgggaaaatc tttcaggatt
     9421 gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa aaacgttttg ctgtagtcaa
     9481 tgagaattat gcttctagtt caaaaagctt attccgtttg atggctgttg cacaaccggg
     9541 tttttatttc ttatttaata tcgtaatggt attgatcatc tggagcgggg cagtgcaaat
     9601 cgatgcaggc gagcttcgtg taggagattt gattgcgttt atcgagtata tattccatgc
     9661 cttgttctca ttcatgttat ttgctagtgt gttcatgatg tatccaagag cagctgtttc
     9721 agctggacgt atccaagaag ccctagatgc caaaccttcg attgtagaag atccaaatgg
     9781 tatatctgat acaaaaacta aagggtatat cgaatttcaa aatgtaacat ttgcttatcc
     9841 aggccattcg caagagccag tgatacgaaa tgtcagtttt acagcctctc caggagagac
     9901 cgttgctttt attggaagta ccggtagcgg aaaatctacg ttgatccaat tgatccctcg
     9961 cttttatgat gtgagtgaag gaaagatctt gatcgatgga gtagacgtga gagattatcg
    10021 gctaagcaag ttgcgagata agattgggtt tattccgcaa aaagcgctgc tatttactgg
    10081 aacgattgca gataatttgc gatacgggaa agaagatgct acgcaagaag aaatggaacg
    10141 tgcagctgag attgctcaag cttcagaatt catttctcga aaaccagatg gatttaatga
    10201 acatttatca gaaggtggtt ccaatttttc aggtggacaa aaacaacgat tggcaatagc
    10261 cagagccatc atacgccgtc cggaaatcta tatttttgat gacagctttt ctgcattaga
    10321 ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa gaaacgacag aatctactgt
    10381 attgatcgta gcacaacgtg tgggaacgat catgcatgca gataagatca tcgtattaaa
    10441 tgaaggagaa gtcgtcggta tggggacgca tcgggagttg ttggaaaaat gcccgattta
    10501 ttatgatatt gccgcttctc aattatcaaa ggaggaatta gcatgaaaaa aaccttttct
    10561 tccttaaaac gtttagctcg atatatcaga ccgtatcgtg agacttttct tttagtgatc
    10621 ctatttacag ttctgacagt ggctttcaac gtggctttgc catatgttac aggacttcct
    10681 actacagaaa tcagccgtaa tattgccaat ggcgaatcaa tcaatttttc ttatatattc
    10741 aaatgtctca tttggatcac agttgttgga atagggtatt gtatttcgca attactttct
    10801 ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg atttgagaag agacatagaa
    10861 gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa accagcaagg aaatattttg
    10921 tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt tacaacaagc ttttattggt
    10981 atcgtaaatg cgatattggg aattacgttg gctgcagcaa tgatgtttta tatccagccg
    11041 atgatggcgc tgatctctat gatcatgatt ccaacatcga tctggatctc aaaacgagtg
    11101 atcaatgctt cgcagaaata ttttcaaagc atgcagaatt ctcttggtga gctaaacggc
    11161 tacgtccaag aaaatatgac aggttttagt gtattgaaag tatatggacg cgaaaaagaa
    11221 acattggaag gattcaaaaa agttaatcac agcttgaaat actttgggtt tcgtgcagca
    11281 ttcatttctg gcttgatgat gcctctagtt caattaactg cctatgctac ttatattggt
    11341 atggctgttc taggaagctt ttatgttatt actggggcga tcgttgtcgg tcaactacaa
    11401 gcattcatcc aatatatttg gcaaatcagt cagccaatgg ggaacgtgac acaattgtct
    11461 tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat tcgagatttt agatgagcca
    11521 gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa cgattttagg agatgtgaca
    11581 ttcgatcatg tcgattttgc atatgatccg aagaaaccat taatccaaga tttgaatttt
    11641 gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga ccggtgccgg caagacgaca
    11701 ttgatcaatc tattgatgcg tttttatgac gtagacaaag gagcaataag gattgatgga
    11761 atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc tatttggtat ggtcttgcaa
    11821 gatgcatggc tttatcaagg aacgattgca gataatatcc gcttcggaaa gcttgatgcg
    11881 acagattatg aagtagttga tgctgcgaaa acagcaaacg tagatcattt tatccgaacg
    11941 atgccagatg gatatgatat ggccatcaat tctgagggag acaatgtgtc tttaggacaa
    12001 aaacagttgc tgacgattgc ccgagcagtg gtctccgatc ctaaaatctt gattttagat
    12061 gaagcaacaa gttcagtaga cacccgttta gaagcgttga ttcaaaaagc aatggatcgc
    12121 gtgatggagg gacggacaag tttcgtcatc gctcaccgtt tatcgacaat ccgtgatgca
    12181 gatctgattc tagtgatgga tcaaggtcag atcattgaaa aaggaacgca tgagagttta
    12241 ttggcacaag gtggatttta tgaaaaactg tataacagcc aatttgctga ggaagcagaa
    12301 taagtcatat aagaataaaa cagtttaaaa cttatttttt gttctctatt cttgataatg
    12361 gtaaagtaaa gttatcaaga aaagtgagga cacggagatg gaacagtttt taaatgaaaa
    12421 aattactgaa gccaaaagag aagttgatcg tttaacgcac gtctgtcaga cgaagccttt
    12481 tgattcagct gatttttttg aaaatgagtt tcaaatcgaa cgcttggcag cacaaataga
    12541 agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat atagatgtac aataaaaaca
    12601 ggt
//
LOCUS       DACDHJ010000063        12424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDHJ010000063 DACDHJ010000000
VERSION     DACDHJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.065"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            151..471
                     /locus_tag="IV223_001338"
     CDS             151..471
                     /locus_tag="IV223_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711637.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            923..1123
                     /locus_tag="IV223_001339"
     CDS             923..1123
                     /locus_tag="IV223_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8711638.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1270..4059
                     /locus_tag="IV223_001340"
     CDS             1270..4059
                     /locus_tag="IV223_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8711639.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4106..4633
                     /locus_tag="IV223_001341"
     CDS             4106..4633
                     /locus_tag="IV223_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8711640.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4654..5844
                     /locus_tag="IV223_001342"
     CDS             4654..5844
                     /locus_tag="IV223_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8711641.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5884..7182
                     /gene="asnS"
                     /locus_tag="IV223_001343"
     CDS             5884..7182
                     /gene="asnS"
                     /locus_tag="IV223_001343"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8711642.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8235..8909
                     /locus_tag="IV223_001344"
     CDS             8235..8909
                     /locus_tag="IV223_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8711643.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8906..9247
                     /locus_tag="IV223_001345"
     CDS             8906..9247
                     /locus_tag="IV223_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711644.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9277..9636
                     /locus_tag="IV223_001346"
     CDS             9277..9636
                     /locus_tag="IV223_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8711645.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9839..10018
                     /locus_tag="IV223_001347"
                     /pseudo
     CDS             9839..10018
                     /locus_tag="IV223_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10160..12241
                     /gene="cadA"
                     /locus_tag="IV223_001348"
     CDS             10160..12241
                     /gene="cadA"
                     /locus_tag="IV223_001348"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8711646.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4149 a         2155 c         2679 g         3441 t
ORIGIN      
        1 tatagagcct aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac
       61 aaaaaacatc atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat
      121 tgggaaaaac ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg
      181 tttctatccc taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct
      241 attacaggca cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg
      301 attcctgaaa cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg
      361 acctgtgatt atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt
      421 actcatactc agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca
      481 aaaaagaaaa actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca
      541 taggctatca gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga
      601 ccgttgaaac agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac
      661 atcagagcaa aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt
      721 ttcactttat gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc
      781 ttttttgtaa acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc
      841 ttgtttcaaa taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg
      901 atacacaagg aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa
      961 aggatttgga ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat
     1021 ccaaggcgac ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga
     1081 aggccaacgc ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa
     1141 ataaacgccc agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg
     1201 agagtatttt tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag
     1261 gaagatattt tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat
     1321 ccaaaagaag atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact
     1381 tcacgttttt cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta
     1441 acagggatca cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg
     1501 atttacaatc ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat
     1561 tttttgaacc atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat
     1621 acagtagaac ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg
     1681 tcagaaagcc ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg
     1741 acgggtcaac ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta
     1801 gagcagattg cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat
     1861 gtgattgaag aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat
     1921 gggctagctt tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg
     1981 acatatccaa gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga
     2041 aaagaacaga acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa
     2101 gatttgatga tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg
     2161 agttatttgg ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag
     2221 cagcagatca tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa
     2281 cctgtaatta tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt
     2341 gtggaaccgc cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg
     2401 acgcaaacga agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc
     2461 tttgcagata tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa
     2521 caagactttg ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat
     2581 catgcgtttt taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt
     2641 ttgttgatcg atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga
     2701 ttaagtatta actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt
     2761 gaccgaatac aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat
     2821 cgagaagaac tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt
     2881 ttagtgagcg gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag
     2941 atgttggatc agctgccact ggaatctggg acagccattg atcacctgca gcttttttat
     3001 caggagatcc agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc
     3061 tggacaaacc agatgcggat cgatttagca agtttatata gcttatttga tcaattagac
     3121 aagcagtact tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg
     3181 ttcgttcctg ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct
     3241 atgttgccgt cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg
     3301 ctgaagatcg gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct
     3361 cttaaagtga ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa
     3421 gaggcacctg ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca
     3481 cttacaggtc tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat
     3541 gttttgcaga aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata
     3601 ctagctcaag ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa
     3661 aaaagcgtat tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct
     3721 ttgcagatcg ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa
     3781 gctaggaacg cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct
     3841 aaagcagctt tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt
     3901 ggcgtattgc ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc
     3961 catgcattac cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac
     4021 atccatgaat tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa
     4081 ttaaaatctg ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa
     4141 acagacaaag gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc
     4201 ggtcatcgtg atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa
     4261 gaaagaagca gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta
     4321 ttggtttact cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga
     4381 aatcgccgtt atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg
     4441 actgactgaa aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga
     4501 aaaagctgct ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac
     4561 aaatgggaaa ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat
     4621 caagggaatt taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga
     4681 ttagaaccgt ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc
     4741 agagatgtgc tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa
     4801 gaagcagcaa tcgaagcaat ccgcaacggg aaagctagct attatacacc aacagcagga
     4861 atcccagagc ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat
     4921 gaaccttcac aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa
     4981 acgatccttg acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt
     5041 gagcaagtaa aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc
     5101 tggaaagtta ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc
     5161 atcaattctc catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc
     5221 ggtgaatggg cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta
     5281 gtttataatg gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag
     5341 actatcatca tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat
     5401 gcggtgggaa atcctgtgat catcaatgga atgatcgctg ttgcttccca gtcaaccagt
     5461 aatccgacag cagtcagtca atatgcagct gttgaagcat tgaaaggcga gcaagataca
     5521 gtggaagaaa tgcggaacgc ctttgaagaa cgcctgaata cgttatattc gcttgtggca
     5581 gaactgccgg gtgtttctct tgaaaagcca caaggagctt tttatctttt tcctaatgta
     5641 aaagaaacgc tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta
     5701 gaagaaacag gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc
     5761 atgagttatg ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt
     5821 attgagagta aaagtcaaaa ttaatcctaa agaaattcaa ggaatataaa aggagagtca
     5881 tacgtggaac aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc
     5941 ggcgcttggg tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac
     6001 ggaacagcct ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa
     6061 acagctaaaa atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac
     6121 agtcgttcaa aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc
     6181 cacgattatc cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat
     6241 ttatggctgc gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt
     6301 gcaacgtatg aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact
     6361 ggttctgcac cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct
     6421 tatttatctc aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc
     6481 ttttcattcg gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa
     6541 ttctggatga tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag
     6601 gaacaatatg tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat
     6661 gtactaggac gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt
     6721 acttatgatg atgcgatcga attactgaag aaaaatggat ttgatgacat cgaatggggc
     6781 gaagactttg gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt
     6841 atcctaaact atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa
     6901 gacgtggtga tctgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga
     6961 agcgaacggg caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat
     7021 gaaaaagaat attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt
     7081 tttggtctag gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa
     7141 gctattccat tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg
     7201 ttctgcatca agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc
     7261 gggacttatt accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa
     7321 gctgtttagt ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat
     7381 aaatttggct taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgcg
     7441 cctgccgttc agctccgagt cacaaggagt aatttcaaaa aacagttttt catgtttgat
     7501 gactattgtg acttgtgacc gaggagttgg ccggagagca ccgtttattc taaaaaagaa
     7561 ctgcgaactc tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa
     7621 gagaagaaag caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc
     7681 aatcaaacat ttacaaagaa ggtaattcaa atggaaatct gatttgatga tgcttctgct
     7741 gttgaaataa agcagcatct agcacctgaa aacaaactaa tagtaacatt cgaagatgga
     7801 gtaggtccgt attttcagca tgcaataatc catatgtaag ttcaattcac ccttgaaatc
     7861 atcggaccag ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa
     7921 agggacattt actagaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca
     7981 gtggtctgat tgacggaagc ttaggtgtga tcgatctctc tggaccaaac aacgaaggaa
     8041 tcaaggggaa tccggcacgt taagaaaaaa atagcataaa aaactagaca cttgatgagc
     8101 gttcattcaa ttgtttgaag ggactaggct ggtgtcattt tttatacata tattttctct
     8161 ttcgtcttga tagggcttat gggataatat aaggaagaaa atcatcgttt tattcaaaaa
     8221 ggagtttttg acaaatgaaa aaacgtatat tatttatcgg tgatagcatt acagacgcag
     8281 gaagagaccg agcagaagca actgatctag gtaaaggtta tccacttttg attgccggaa
     8341 aactaatctc ccaatatcca acgatggaat ttgaatgcat gaaccgtggg ataggaggag
     8401 acaaaattgc agatctgcaa gtacgctggc aagaagattg tttagcttta caacctgact
     8461 atgtatcgat tttgatcgga atcaatgata cctggcataa tactagcgat caggctatat
     8521 tcggaactga gaaatctgct cagcaatttg aatccgttta tcgaaaatta ctaaatgaac
     8581 ttcaaacagt gggaatctgc aacatcatct tgatggagcc gtttgtactt cctataaccg
     8641 acgaccgtca tggctggcgt aaagacttag accctaaaat ccaaattgtt cgtaaacttg
     8701 ctaaggaata tggcgcattg ctggttcctt tagatggtat attcaatgct ttagggattg
     8761 tttatggttt ttcgaaatat acaagagaag atggggtcca cccaacgatt ggcggacatg
     8821 agatcattgc acaaagctgg atagatgcaa ttgccgcagt cgattttttt agtggagata
     8881 aaaatgagcg aaagaaaagt gagaaatgat ggaaatacgg tgggcagtaa aagaagatct
     8941 acctcaaatc aatcagctga ttactgatca tccagaatca ttcgtaaaag caaaaacggc
     9001 agctgaccta aatgataaga ataaactatt gctcgttgct gtaacaaaag aaaaacggat
     9061 tatcggattt gcgcaagcgg acgtgcaaca gtcagaagaa cgattggatc agcgaccatc
     9121 aggaaaacta atccaacaca cattagtaac aaaagaaaat acggaagaaa tccaaaaaaa
     9181 atttgcagaa tatttgatac tggaagctaa aaaaagaaga ataaaatcat tttttgccca
     9241 gttttaatca aaaacagtaa tagaaggaga aatacaatga gttgtttagg aaatgtcatc
     9301 tggtttatat tcggcggact aatcggtggt cttagctggt tagcagcggg gatactttgg
     9361 tgtatcacga tcataggtat cccaatcggt atgcaatgtt ttaagatcgc ctccctttct
     9421 tttgcgcctt ttggcaaaga agtaatttat caccagtctg gcatgtcatt gattgttaat
     9481 atcatctggc tgatcgtttc aggcctacca attgctctcg cacatgtcgt cagcggcatc
     9541 cttctatgta tcacgatcat agggatacct tttgccaagc aatctttcaa acttgctcga
     9601 ttggcgttga tgccattcgg tgcagaaatt gtataaaaga gaataataaa ggttagataa
     9661 agcatagagt caggtgggat acatgtattt cacctgactc tatgcgttct cctgtataag
     9721 ttcttccatg tgacttttac gtcttttatt tatatggggt tactgtttat ggaaagttga
     9781 aataattttc attaaagaac tctttctata tttttgaaag cgtagtggag cttcgaatat
     9841 ctgggaagga cattttgcag ggattgaaaa aagatattgt cctatcgaaa aattaatgaa
     9901 tttagacaaa ttgccagcaa caggcttcaa acttagttgc tatcctgtca agatcaagaa
     9961 ggcaagtgcg ggttggataa gagcagtggc aatgatcgaa aagaaaaaga aagaataagc
    10021 caaaacatct gtatggaaga aattttccga tgcagatgtt tttcattttt tctttcggat
    10081 accttgacat atgaataacc gttcatttat aattagttat gaagagatat tcatatgttg
    10141 aggcgaggag gtcattaaaa tgaaaagtta cagattagaa ggtttagatt gtgcgaactg
    10201 tgcaatgaaa atcgaaaaag gagttcagaa gatcaatgga gtcaaggaag caacggtaaa
    10261 cttcacttct ggaaaactaa cgattgacgc agaggaagat catttggcta cgattgaaca
    10321 agaaataaaa aaagtagtga aggaattaga accagatgtc aaagtaacag aaatagataa
    10381 ggaaaaagta tctgaacatg gaaatgaaaa agagagaaat acgctgttcc ggattttatt
    10441 ttcccttgca ggtatcgctt tattgctatt atttgatttt aatgaaccaa ttcgcttgat
    10501 agggtattta ttgatttatc tgctgatagg atatgatgta gtgaagaagg cagtgatgaa
    10561 tatcgtcaaa ggaaaaatct ttgatgagaa ttttcttatg tctgttgcta cgattggcgc
    10621 tatgttgatc ggtgaatatc cagaagccgt agcagtgatg ctattttatc aaatcggcga
    10681 gtatttccaa ggattggcag tcagtcattc aagaaaatcg attcgcgaat tgatggcgat
    10741 ccgtccagaa acagctcacg tgcagacgac ggaaggattg atgaccgtga accctgaaga
    10801 cgtgcagatc ggtcagtttg tacttgtgaa gcctggtgaa cgtgtaccgt tagatggaac
    10861 gatcatcgaa ggagaatcat tagtcgatac gtcagcactt acaggagaat cagtccctaa
    10921 atctgtatat gttggtgaaa cagtattaag tggatttatc aataaaaata aaccattttt
    10981 agttcaagta gaaaaatctt atgaaaattc tactatcagc aaattattag aattagtaga
    11041 aaatgccagc agtaaaaaag caccagctga aaatttcatt actaaattcg cgcgctatta
    11101 cacaccagtc gttgtaggat tggccgtttt attagctgtt ttacctccac ttgttgtttc
    11161 tggagcggcg ttttctgaat ggatctatcg agcattgaca tttttagtga tctcatgtcc
    11221 atgtgcatta gtgatctccg taccccttag tttctttggc ggtatcggag gagcaagtaa
    11281 aatcggtgta ttagtaaaag gttcaaatta cttggagttg cttgcacaaa ctgagacagt
    11341 ggtattcgat aaaaccggca cattaacgaa aggtgacttt tcgatccaga cgatcgatac
    11401 aaaaatcgat ccaaagatat tcatgcaata tgttgcgagt gcagaacagt tttctacaca
    11461 tccgattgct caatctgttt tagaaggcta cgcaggtccg cttttaccaa cagctaatat
    11521 ccaagaattt gcaggtgaag ggatcttagc tgaagttgat ggcaaacaag tattagcagg
    11581 taatcacaaa ttgatggaaa gatttgaaat ctcttttcct tcaagtcaag aaatcggtac
    11641 attgctttat ctagcgatcg atcaaagtta cagtggttat cttgttattg cagatacctt
    11701 gaaagaagat gccgtagacg cattggttca attaaaaaag gctggtgtga agaacacagt
    11761 catgttgacg ggcgattcga aaaaaattgc tgatcatatc gggaagcaag taggcgtgga
    11821 caagatatat agcgaattgc ttccagaaga taaagtgcag cggttagagg aaatcttaca
    11881 aagaaacaat aaaaagaccg catttgtcgg agatggaatc aacgatgcac cggtgctagc
    11941 aagagcagat gtaggaatcg caatgggtgg attgggaagc gatgctgcaa ttgaagcagc
    12001 agatgtagtc atcatgaatg atcaaccatc gaagattgca gaagcaatcc atttagcaaa
    12061 gaaaacattg aaaatcgtca aacagaacat tgtctttgct attgggataa aaattttggt
    12121 acttgcctta ggtgcttttg gttttgcatc aatgggagat gcagtatttg cagatgttgg
    12181 ggtgacagta ttagctgttt tgaatgccat gcgaagcttg catgtaaaaa ataaacgttg
    12241 atcaatgaag aggacgaatg aacgtcctct tttttttata tccttttctt aatgaaaaat
    12301 gtaaagaaaa tagataggat tataaaataa ctaatgtttt taattttgca gaaaattcaa
    12361 aatagcttga aattcaataa ggtaaatgaa ataaaagaaa ataacttatt tttttgatgg
    12421 aaaa
//
LOCUS       DACDHJ010000064        12287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDHJ010000064 DACDHJ010000000
VERSION     DACDHJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.066"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..779)
                     /locus_tag="IV223_001349"
     CDS             complement(<1..779)
                     /locus_tag="IV223_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711647.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIK"
     gene            917..2431
                     /locus_tag="IV223_001350"
     CDS             917..2431
                     /locus_tag="IV223_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8711648.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2834..3817)
                     /locus_tag="IV223_001351"
     CDS             complement(2834..3817)
                     /locus_tag="IV223_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8711649.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3822..4469)
                     /locus_tag="IV223_001352"
     CDS             complement(3822..4469)
                     /locus_tag="IV223_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8711650.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4554..5186)
                     /locus_tag="IV223_001353"
     CDS             complement(4554..5186)
                     /locus_tag="IV223_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8711651.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5202..5753)
                     /gene="coaC"
                     /locus_tag="IV223_001354"
     CDS             complement(5202..5753)
                     /gene="coaC"
                     /locus_tag="IV223_001354"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8711652.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5750..6487)
                     /locus_tag="IV223_001355"
     CDS             complement(5750..6487)
                     /locus_tag="IV223_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8711653.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6484..7473)
                     /locus_tag="IV223_001356"
     CDS             complement(6484..7473)
                     /locus_tag="IV223_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8711654.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7731..8273
                     /locus_tag="IV223_001357"
     CDS             7731..8273
                     /locus_tag="IV223_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8711655.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8290..9375
                     /locus_tag="IV223_001358"
     CDS             8290..9375
                     /locus_tag="IV223_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711656.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9387..10193
                     /locus_tag="IV223_001359"
     CDS             9387..10193
                     /locus_tag="IV223_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711657.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10194..11021
                     /locus_tag="IV223_001360"
     CDS             10194..11021
                     /locus_tag="IV223_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711658.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11018..12091
                     /locus_tag="IV223_001361"
     CDS             11018..12091
                     /locus_tag="IV223_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8711659.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRK"
BASE COUNT         3847 a         2214 c         2543 g         3683 t
ORIGIN      
        1 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
       61 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
      121 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
      181 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
      241 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
      301 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
      361 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
      421 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
      481 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
      541 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
      601 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
      661 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
      721 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
      781 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
      841 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
      901 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
      961 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     1021 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     1081 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     1141 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     1201 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     1261 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     1321 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     1381 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     1441 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     1501 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     1561 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     1621 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     1681 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     1741 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     1801 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     1861 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     1921 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     1981 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
     2041 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
     2101 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
     2161 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
     2221 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
     2281 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
     2341 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
     2401 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
     2461 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
     2521 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
     2581 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga
     2641 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
     2701 ggcggaagca gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
     2761 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
     2821 gatcagcatg attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt
     2881 ccatatattc atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc
     2941 gaatcttgcc attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg
     3001 ctttatactg atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa
     3061 tcaatacacc aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc
     3121 aacgtccatc aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat
     3181 ccaaaataac atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt
     3241 gttttttata gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt
     3301 ctgatcctgc agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg
     3361 ctgttcccac accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc
     3421 caagccagta aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa
     3481 catagtctag cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg
     3541 cataaccacc tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat
     3601 cacttttatt ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt
     3661 tcaaagagac tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt
     3721 tgatcagtaa ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat
     3781 ctacagaacc aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat
     3841 tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa
     3901 atatccgttt cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt
     3961 tttgatgcct tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc
     4021 aatgtgactg gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca
     4081 taatgtttag taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca
     4141 taaacagcac tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt
     4201 ttgattgcgc catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa
     4261 atcaatacat cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca
     4321 aaagagacat acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc
     4381 cctgcaaaaa tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt
     4441 ccgtttgcac ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc
     4501 attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta
     4561 gtaagagaag ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga
     4621 cctgcgacga ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa
     4681 acagcattta aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc
     4741 atattcatca ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa
     4801 acggaatctg ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc
     4861 accccaatca agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct
     4921 gttccgatga ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg
     4981 ctacggatca tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct
     5041 aaaacgatcg aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata
     5101 gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt
     5161 aatgtaaagt tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta
     5221 agagtcagca tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa
     5281 tctccacaag ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat
     5341 gtttccagat tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca
     5401 atcaattttg gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca
     5461 atgccgtttg ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca
     5521 gcttgttttg ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct
     5581 gtatggactg cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg
     5641 cttttagaca taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca
     5701 gcgcttttgt aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt
     5761 cctccagtct aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt
     5821 ccactgttcc atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca
     5881 aatcatttgc gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca
     5941 gaagagattc ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct
     6001 gccattcacg tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct
     6061 cggtgttaga agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat
     6121 tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa
     6181 aactaaaagc aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt
     6241 cttttttcgt tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa
     6301 tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg
     6361 tggtatccgg attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat
     6421 gatttgtaat agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa
     6481 tcttcattta tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag
     6541 tttagatgct gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc
     6601 aatctgcaaa tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac
     6661 agaatcaatt ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag
     6721 tttgattccc ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag
     6781 aagactgctt agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc
     6841 taaagctttt gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag
     6901 actttttagc catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc
     6961 tagtttatgt aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac
     7021 aactttttta tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt
     7081 aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac
     7141 taaccagtct cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag
     7201 cccaaaacca gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac
     7261 gacagttccg gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga
     7321 ctcgctcatt gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac
     7381 ttcattttca gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc
     7441 ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc
     7501 ttttatcata ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa
     7561 ttaggtgaaa aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc
     7621 agcaaaaaaa attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt
     7681 ggtagtaaac aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg
     7741 gtgagaagtt aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac
     7801 gcacagattt gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa
     7861 tggaaacatt cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc
     7921 aagaagcagc gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg
     7981 aagaaaacgg gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc
     8041 ctgtcttgct gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg
     8101 aaacgtttat ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg
     8161 tggcaaaagc aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc
     8221 atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg
     8281 ggtaaaaacg tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat
     8341 gaaacagtat taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt
     8401 ggtccttccg gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct
     8461 acagaagggg acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga
     8521 caagtaaata cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac
     8581 gttgcttttg gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa
     8641 gatgctttgc gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct
     8701 ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg
     8761 ctattagatg aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa
     8821 ctgcgggatc tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa
     8881 gaagcgttgg caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt
     8941 ggtacacccg tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg
     9001 gagagcaata tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag
     9061 caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc
     9121 ccagaggatt tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact
     9181 cagctgtttc gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg
     9241 atggtccatt caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca
     9301 gaagatatcc atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac
     9361 agctacgaag aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt
     9421 atattttgtg gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt
     9481 tttttgatat gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga
     9541 catcgctttc tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc
     9601 tgatcatcag ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc
     9661 tgatgctgat tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg
     9721 ggattttcag tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga
     9781 aacagatatt attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat
     9841 ttatgattat gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta
     9901 gtagagattt aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat
     9961 taaatggtgt gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc
    10021 tgactcgtct gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt
    10081 tcttggttac acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta
    10141 tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa
    10201 aatttaaatg gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct
    10261 tctatctgat tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta
    10321 cttgggaaaa ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata
    10381 cgttcatgct ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta
    10441 tgggaatcta ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca
    10501 tcttattagt ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac
    10561 taggattcgg attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa
    10621 tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg
    10681 ctagagacct tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt
    10741 ctccaggaat catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg
    10801 ttacattttt cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg
    10861 caagacaagg aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca
    10921 tgatattggt cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga
    10981 aaaacagaag agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt
    11041 tttaggtatt ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac
    11101 aagtggcatg agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc
    11161 tagtttgatc acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga
    11221 ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat
    11281 accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca
    11341 ttcgaagatc aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc
    11401 agataatcag tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa
    11461 attctttgat gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga
    11521 cagtttgatg ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg
    11581 ctattctttg aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca
    11641 tttaacacca aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga
    11701 agaagcctcc gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga
    11761 acatttacat tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat
    11821 gccaaaaacc gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc
    11881 ggaaaatgcg gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa
    11941 agcgttattg ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat
    12001 ctcccatctt gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct
    12061 attcttagaa ttcaagatgt atcggaaata gcgagaaaat aagcaaattt gcataactat
    12121 aataaaaagg acttagtttt gatgggaaat ttttcaaaat taagtccttt tttatgtggt
    12181 tgaaacgggt atgatcattt taaggaagtg atctaagatg aaaggacgac tgaacaatta
    12241 tcatattaga aaagctttat tgtattccac aaacaaatta ctaagaa
//
LOCUS       DACDHJ010000065        12272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDHJ010000065 DACDHJ010000000
VERSION     DACDHJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.067"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..581)
                     /gene="frr"
                     /locus_tag="IV223_001362"
     CDS             complement(24..581)
                     /gene="frr"
                     /locus_tag="IV223_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8711660.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(584..1306)
                     /locus_tag="IV223_001363"
     CDS             complement(584..1306)
                     /locus_tag="IV223_001363"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8711661.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1442..2323)
                     /locus_tag="IV223_001364"
     CDS             complement(1442..2323)
                     /locus_tag="IV223_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8711662.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2422..3204)
                     /gene="rpsB"
                     /locus_tag="IV223_001365"
     CDS             complement(2422..3204)
                     /gene="rpsB"
                     /locus_tag="IV223_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8711663.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3563..4042
                     /locus_tag="IV223_001366"
     CDS             3563..4042
                     /locus_tag="IV223_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8711664.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4259..5350
                     /gene="phnW"
                     /locus_tag="IV223_001367"
     CDS             4259..5350
                     /gene="phnW"
                     /locus_tag="IV223_001367"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8711665.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5343..6128
                     /locus_tag="IV223_001368"
     CDS             5343..6128
                     /locus_tag="IV223_001368"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8711666.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6474..8165)
                     /locus_tag="IV223_001369"
     CDS             complement(6474..8165)
                     /locus_tag="IV223_001369"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8711667.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8587..9534)
                     /gene="arcC"
                     /locus_tag="IV223_001370"
     CDS             complement(8587..9534)
                     /gene="arcC"
                     /locus_tag="IV223_001370"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8711668.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9649..10668)
                     /gene="argF"
                     /locus_tag="IV223_001371"
     CDS             complement(9649..10668)
                     /gene="argF"
                     /locus_tag="IV223_001371"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8711669.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10759..11988)
                     /gene="arcA"
                     /locus_tag="IV223_001372"
     CDS             complement(10759..11988)
                     /gene="arcA"
                     /locus_tag="IV223_001372"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8711670.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3370 a         2540 c         2195 g         4167 t
ORIGIN      
        1 gcccctctgt ttttatgatt ttattaaact tccaataatt ctttttcttt atcagcagcg
       61 atcttatcta cttctttaat gctgttatct gtcaatgttt gtacgtcttt ttctaatccg
      121 cgcaaatcat cttcggtaat atcaccattt ttttgttgtt ttttatattc atcgatcgca
      181 tcacgacgga tgttacgaat cgcgattttt gcattttctg cttctttttt cacatcttta
      241 gctaattctt ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat gacatttcca
      301 tcattggttg gactgattcc gatatcactt gccataatag ctttttcaac atcttgaaga
      361 atgctcttgt caaaaggtgt gatcattaaa acacgtgctt ctggaatttg gattgatgcc
      421 atttggttca atggtgttgg cgcaccgtag tagttcacag taatacggtc caaaaggcta
      481 gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt gaagattttg tgctgctttt
      541 ttcatttttt cttttgcttc tgtcataatt gcatctgcca tttttatttc ccccttacag
      601 ttgttccgat attttcgcct aagattgcac ggcgaatatt tccgtgttca tttaaattga
      661 agacaagcag tggaatatca ttatccatgc ttagtgaact tgcagttgaa tccatgactt
      721 gtaatccttt ggcaataact tccagatgag tcagttcttc aaattttact gcatttgcat
      781 ctactttcgg atcagcagaa taaacaccgt ctacattgtt tttagccatc aggataacat
      841 ctgcgccgat ttctgccgct cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc
      901 cggtaccgcc tgcaaagata acgatccgtc ctttttctaa atgacgttct gctttacggc
      961 gaatatatgg ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac
     1021 ccacgttttc caacgtatcc tgtaaagcta aggcattcat aactgtagct aacataccca
     1081 tgtaatcagc ttgagcacgt tccatgccca tatgtgctcc gatttgtccg cgccagatat
     1141 ttccgccacc cacaacgatt gccatttcaa cgcccagctc gtgaacttct ttgatttctt
     1201 ggatgatttc tttgattact ggtggtttga ttccaaatcc tgcatcccca gctaaagctt
     1261 ctccactcaa ttttaagact acacgctgat atttcggtgt gaccatgttc attcctccaa
     1321 tattttctat tgccatttta ccatacttag atgatttcga cactctaaga ctggtttttc
     1381 ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga
     1441 attatttttt cacttggctc ataacttctt ctacaaagtt atcttcgcgt ttttcgatac
     1501 cttcgccaac ttcgaaacgt acgaatgttt taacagttgc gccttttgat gcaacgtatt
     1561 tttcaacagt catatcagga tctttaacga atggttggtc caccaaagcg atttctgctt
     1621 tgaatttctt caagcgtcct tctaccattt tttcaacgat attcgctggt ttgccttcgt
     1681 tcaatgcttg ttctgtaaga acagtttttt catgttctaa ttcagcttct ggaatttgtg
     1741 attcgtttac ataacgagga ttgatagctg ctacgtgcat agctacgtct cttgcaactg
     1801 tttcgtcagt tgttccatct agaacagtca atacggcaat acgtccgccc atatgcaagt
     1861 atccaccaaa tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg aagctgattt
     1921 tttctccgat aacttgagtt gcttcgatca agtcagattc aattgtacct ttatctgttt
     1981 tgattttcat tgcagcttcc atgtctgctg gtttgttttc agcaacaagt tctgcaattt
     2041 cttttactaa atcttggaac atttcgtttt tagaaacaaa gtctgtttct gagtttactt
     2101 caacgattgc tgcagtgtta cctttcacag caactgaagc caatccttca gctgcaatgc
     2161 ggtcattttt cttcgcagcc tttgccattc ctttttcacg taaaagatca actgcttttt
     2221 ccatgtcgcc ttctacttca actaatgctt ttttcgcatc catcattcct acgccagtca
     2281 tttcgcgtag ttcttttacc attttagctg taacgtctgc catgtttaac tcctcctgtg
     2341 tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg
     2401 aaacagctcc atcattcaaa attattctgc tgaagaattg tcgccttcaa caacatctac
     2461 gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc
     2521 ttcaccttgg tttccttcga tgaaagcatc agccattttt gaagtaatca atttaacggc
     2581 acgaattgca tcgtcgtttg aagggattac tacgtcgatt tcatctggat cgcagtttgt
     2641 atcaaccata gctacgattg ggatatttaa tttgtgtgct tcttgaacag caatacgttc
     2701 tttacgtggg tcaacgatgt acattacatc tgggattctt ggcatatcag cgataccgcc
     2761 taagaatttt tcaagacgtt cacgttcttt gttcaaacca gcaacttctt ttttaggtag
     2821 aacttcaaaa gttccatctt cttccattgc attgatttgt ttcaaacggc tgatacgttt
     2881 ttggatagta tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg
     2941 tcctgcacga gttgcttctt ctttgattgc ttcttgtgct tgtttttttg tacctacgaa
     3001 taatgctacg ccgccttctt cagcaacgct cttcatgtaa tcataagctg catctactaa
     3061 tttaactgtt ttttgcaagt caatgatgta gattccgttt ctttctgtga agatatattt
     3121 cttcattttt ggattccagc gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa
     3181 ttgtttcata gaaattactg ccatgtttgt ttcctccgat ttggttttat ttttcctctt
     3241 cttatcttct accttgccgg caaacttttt caagcaccta ccgacaatcc aataagaatg
     3301 tggatttgct gatgcataaa gcaccctcag ctattataca gacaaaagat ccgtatcgca
     3361 agagcaaata tacttttctg ctcaacattc aatagtttac ctgaaagata aaaagaattc
     3421 aagaaaaaca gtagatttct ctttgtttct ttattatcct ttttccataa aataatttta
     3481 ggtataaaag acgaatatta ttgggcaatc cttctttttt ttgttatgat acatgtgaaa
     3541 tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga catttgctga ttaaacaagt
     3601 cattgaagaa ttcccgatcc gtacgcaaga agaactatta aacaaacttg ggcattatgg
     3661 cgttaccgcc acgcaggcaa cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc
     3721 acctgatgaa aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac
     3781 taggactgag gaagaggatc ggctggttca aatgattgaa gacattgtat tgaaagtcga
     3841 acgagtccac tttttgacga tcgtcaatac cttgccagac aatgctcatt tatttgcatc
     3901 tgttttagat gagatcaaac ctccctctat tgtcagtaca attgctggat tcgatacgat
     3961 catggtcatc tcggaaaatg aggaggacgc aaaacgagta gaagaatttc ttcaccatcc
     4021 aactgaagta tcactttttt aatcagagca cgtattccta atatggaatg cgtgtttttt
     4081 tattttcaat attttcctta ttaaataaac ctttacactc tttacaaaaa tacatcattt
     4141 tttattcaat tttttcaaca ccttgattga atcacaataa tcatcattta tattggatgt
     4201 taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat
     4261 gtataaatta ttaactcctg gaccattgac gacttctgaa acagtgaaaa atgctatgct
     4321 gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga taagaaaaaa
     4381 attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa tgcaaggtag
     4441 cggctccttt ggggtagaat cggtattaag ttctgtgatc aggccagaag atactgtact
     4501 gatttgttca aacggcgcat atggacaacg aatgatcaac atgtgcgaat gtctgaaact
     4561 gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg tgattacaca
     4621 gctgattgaa gagaatccct ctatctccgc actagcaatg gtccatagcg agactacttc
     4681 cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaaaag atcttttgtt
     4741 tatagtggac gcgatgtcaa gctttggtgg gataccgatt cgtgtagccg aactagggat
     4801 tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct tttcttttgt
     4861 acttgctaat aaagcggaac tagaaaagcg aaaaaatgtg agtcgaagtt tgtcacttga
     4921 tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc
     4981 tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat
     5041 cgaaactcga tattcacgtt atctcgccaa taatcatgta ctacgacaaa aaatgacaga
     5101 gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct cttttcttta
     5161 ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc aaaacggatt
     5221 tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa atattggcga
     5281 gatctatccg gaagatattt tggaactgac agcaattatc aaagactata aggagaacgt
     5341 taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt ggctgtatgg
     5401 ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aatccaactg actgacaaag
     5461 aaatccgcga accgatggga aaattaaaat gggatcatat ccaacagctt ctttccctcc
     5521 cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa aaagaggtgg
     5581 aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag cattctacct
     5641 tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc aatattggtt
     5701 ccacgactgg atttactgct cctatgatga caatcgtagc agaaaccgca aaaaaagaag
     5761 ggtattcacc agacttcatg gcaacttctg aagacgtcga agggtatggt cgaccgtttc
     5821 cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat gaagtaatca
     5881 aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg ttgacagtag
     5941 gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa aaattagaga
     6001 tggggaaaaa agatcgttta aaacagtctg ttgctcttag attccaacaa gctggcgctg
     6061 atatttgtgt aaacaattta aaagagttaa cagaatacat tttgtacgct gacaagaatc
     6121 cagaatgact tttatacaaa atcagaggtc gtgaaaaagc tgtactgcat caagtaataa
     6181 gaacagccaa acaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga
     6241 tgcaagcttt tgaacaccgt ttattcgaaa aaaaaaggga gctgtgacac gacttttgtc
     6301 acagtcccct tatttacaaa caaacagtgt ttaagagaca acctctccac taaacggtgt
     6361 tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa gctattttct
     6421 cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt ttcttacatc
     6481 ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac agtcactgca
     6541 taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt cgtatgagca
     6601 tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac tgacggttcg
     6661 tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt aaccacttcc
     6721 cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa tcctgctttt
     6781 tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt ttctccttca
     6841 aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg
     6901 tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt ttccaaatca
     6961 gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa tacttcttct
     7021 aataagacga tctttccttt acgagttgat aattttttac caccttgcgt gatcaaacca
     7081 aatggaatat ggcgcatatc gtcagaccag tcaaagccca tttccttcaa gacagctttc
     7141 aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg tttgaagtcg
     7201 taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg
     7261 tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac gatctctgca
     7321 cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc atccatcttg
     7381 tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag atcatagatt
     7441 ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat agcctcttca
     7501 tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc tggttctgtt
     7561 tcagctactt catggaattg gacatataaa cgaagcaatt cattgattgg ttctgcttta
     7621 acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc
     7681 cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat aatgaaaccg
     7741 atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt ggcaatatta
     7801 ggagaagaca tgtcgattgg tactgttcct tgattcccga tattgctatc cccataatgt
     7861 tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt attcatgaaa
     7921 aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc gattttttct
     7981 gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct
     8041 ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg ttcgacttgt
     8101 tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag atctttatta
     8161 ttcatcttta ttcctcctaa agtgctccct tgattgttaa acaaaaaaaa tctctagcga
     8221 cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac tccaattgtc
     8281 ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa aacagaattc
     8341 tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga attcgctagc
     8401 caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa ctttcttgaa
     8461 ttatatcaga aaaaaaaacg aaaggctagt cgtttctgat aaaaaaacaa tccagatggc
     8521 ctaaaggaga agaaatacca cctggatagc tataaatcat ttcgtaaata cgaaagactt
     8581 aacaaattaa tcagctacga taatcgttcc ttcttcagaa gctaaaagat tctcgatatt
     8641 ttctaaagaa gtgatgattg ccttagcgtt tggttttgct tcaacaaatg caatagctgc
     8701 ctctactttc ggaagcatgc ttcctggagc aaattgcttt tcatcaatgt attgtttcat
     8761 ttctgaaaca gtaacagttt ctaatttctt ttgatctggt ttttggtaat tgacatacac
     8821 attgtctact cctgtcaata cgatcagcaa atccgcatcg atgatttcgg ctaatttttc
     8881 tgaagcaaaa tctttatcga ttacggcttc tcgtccttct aatccagtag ctgtttctac
     8941 aacaggaatc ccaccgccac caacagagac tgtgataact ccttgatcga ccagcgtttc
     9001 gatgacacgt gcttctttaa tcgagatggg tttaggggaa gcgactactt ttctccatcc
     9061 acgtccagca tcttctttga atgtcacagt ggagtcagca ttcatttgtt ctttggcttc
     9121 ttcttccgtg tagaatggac cgactggttt acttggattt ttgaatgaag gatcattttc
     9181 atccacaata acttgagtga ctaaagaaac cacatcttta tcgatccctt tttccttcag
     9241 tacttcgccc atggcatttt gtaaccagta accgatagat ccttctgtca ttgcaacaca
     9301 tgtatctaaa ggcattgccg gtgttttctc agaatcagca gcttgctgct gaatcaatag
     9361 gttaccgact tgcggaccat ttccgtggga gatgatcaat tcatctcctt gttcgataaa
     9421 tttcactaaa tactttgctg tttccattaa cgcttcttgt tgggcttttg cacttgcatc
     9481 cgtagacaag attgcattac cccctaaagc gacaactact tttcgtttgg ccatttcatt
     9541 cgtcctttct tcagttctac cgaattttct agataacaaa acgagccagc gttttgtgat
     9601 tttccctcaa tgctgactcg ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg
     9661 gataaacaag ttacctaatg ttgctgccat gatcgctttg attgagtgca tacgattttc
     9721 tgcttggtca aattgacgag catatttgct gcggaagact tcatctgtta cttccatttc
     9781 agtgatgcca aagcgttctt tcatttgttc accgtaaaca gtgtttgtat catggaaagc
     9841 tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta ccatttccat
     9901 attgatttgg taaggtttca acaatttgat acgttcttcg aatttatctt cttcacccat
     9961 tgatacccat acatcagaat aaaggacgtc tgctccgtct acacctttat caacatcgtc
    10021 ggtaatcatc aattgtgcac cggatttttc agcaaagcct tctgccattt tgacgatttc
    10081 ttcttcaggt tgtaattctt ttggtgcaac gatacgcata ttcgctccta aaattgcacc
    10141 tgtgactaat aaagagttag ccatattgtt tcgaccatct ccgcagtaag caacagtgat
    10201 accttctact gttccaaagt tttcttggat agttaaaaag tcagcgatca tttgtgttgg
    10261 atgccattca tcagtcaagc cattccaaac tggaacacca gagaattctg ctagttcctc
    10321 taccattttt tgactgaatc cacggaattc gattccatca aacatacgac ctagtacttt
    10381 agctgtatct tctgtggatt cttttttacc taattggata tcatttgcac ctagatattc
    10441 aggatgtgca cctagatcga tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt
    10501 tttttcaaat aaaagcgcaa tattttttcc ttctagatag tgatgtggga taccacgttt
    10561 tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt cttttgtaaa
    10621 gtctttttct gctaataagc ttcttccttg gaatactgat tctttcatat taaacattct
    10681 cctttagatt gtttttattt aagattaaag aggaaatccc gacggcagct gacagtaaga
    10741 ccgccgtcag gaaacaaatt acttcaagtc ttcacggaca agtggttggc tcatgcagcg
    10801 tggaccacca cggcctcgtg acaattcact tgaattgatt tctaacactt tgatgccata
    10861 gctacgaagc aattcgttcg atacataatt tcggttataa gtgactacaa cacctggagc
    10921 gattgccaat gtgtttgatc catcattcca ttgttctcga ggtgcaacga tctcatctcc
    10981 attaccagtt gggatcagta caagatcatc taatccaaga gcagctttca tcgtttcgtg
    11041 taagttatct gaatgagtaa tcttgatatc atcaccatct ggttcgattg taaagacatc
    11101 catcttaccg tctttacttt gaattgctgg atggatagtg aatttatcgt gatctaccat
    11161 tgtaaatacg gtatccaaat gcatcattgc acggttgttt ggaattttga ttgctaacac
    11221 tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct aatttttcta aagctttcgc
    11281 attagttcgt tgagaaatcc caacagctac tactttatcg cttaagatca attcgtctcc
    11341 gccttcgata tgatctggat tttcacgatc caaccatact tcgattcctt tatttgcaaa
    11401 tcttggatga tgtttcaaaa tatattctgt aaacatcgat tcacgacgac gtgcttcaaa
    11461 agtcatctta ttgactgtca tcccatttcc cattgatgcg gaagggtcgc gtgtaaagta
    11521 caagtttggc attgggtcca tgtaaaatgg atagtcatcg tctgctgaca gatcgtatag
    11581 actgctagaa cgtacttcaa tatcttttgt acgaacccca gccatgattt tatctaccat
    11641 ttcttgcgtt tccattgata gaaggaactc atgaagaccc tctcttacag cattcgatgc
    11701 aatatgagat tcatccaaca ttttattcaa gaattgttct ttgacattgc ctgcgtcaat
    11761 cgcttctgcc gctaattttt ccaagtaaag agtctcgacg ccttcatttt gcaatgtctt
    11821 tgcaaagttg tcatgttctt cttgagcaat cggaagatat ggaatatcat cgaacaacag
    11881 acgatccata atatctggtg tcaaattttc cacttcttgt ccaggccttt tcagtaaaac
    11941 tgtctttaat ttccctattt cagagaaaac gtgaataggt ttatccatgt tgtgttgcct
    12001 ccttcttttt gattacatgt ataggatatc ctccttttgt aatcactttc ataattttgt
    12061 ctcattcaaa atgagacatt cctcacatta atattcgata attaaataat taaacgtttt
    12121 tctattttta agcgtaattt attcattttt tattctgtat acactttcaa aaaaatattt
    12181 attcattgaa aaaaattttt tgaataaatt aaaaaaataa attatattag cgttgttagc
    12241 gtttactata tgtattattg ttgtttttta ta
//
LOCUS       DACDHJ010000066        11938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDHJ010000066 DACDHJ010000000
VERSION     DACDHJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.068"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            592..1920
                     /locus_tag="IV223_001373"
     CDS             592..1920
                     /locus_tag="IV223_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8711671.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IV223_001374"
     CDS             2122..2955
                     /locus_tag="IV223_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8711672.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IV223_001375"
     CDS             2971..3708
                     /locus_tag="IV223_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8711673.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IV223_001376"
     CDS             complement(3863..4831)
                     /locus_tag="IV223_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8711674.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IV223_001377"
     CDS             5063..5896
                     /locus_tag="IV223_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8711675.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IV223_001378"
     CDS             5930..6769
                     /locus_tag="IV223_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8711676.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IV223_001379"
     CDS             6795..7355
                     /locus_tag="IV223_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8711677.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IV223_001380"
     CDS             7554..9275
                     /locus_tag="IV223_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8711678.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IV223_001381"
     CDS             9440..10582
                     /locus_tag="IV223_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8711679.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IV223_001382"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IV223_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8711680.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4064 a         2013 c         2479 g         3382 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tttagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttat
//
LOCUS       DACDHJ010000067        11508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDHJ010000067 DACDHJ010000000
VERSION     DACDHJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.069"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..2154)
                     /locus_tag="IV223_001383"
     CDS             complement(79..2154)
                     /locus_tag="IV223_001383"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8711681.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IV223_001384"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IV223_001384"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8711682.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IV223_001385"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IV223_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8711683.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /locus_tag="IV223_001386"
     CDS             complement(4412..5311)
                     /locus_tag="IV223_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8711684.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5727)
                     /locus_tag="IV223_001387"
     CDS             complement(5326..5727)
                     /locus_tag="IV223_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8711685.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IV223_001388"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IV223_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8711686.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IV223_001389"
     CDS             complement(6198..8390)
                     /locus_tag="IV223_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8711687.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IV223_001390"
     CDS             complement(8414..9385)
                     /locus_tag="IV223_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8711688.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IV223_001391"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IV223_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8711689.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3106 a         2419 c         2103 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacag
//
LOCUS       DACDHJ010000068        11453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDHJ010000068 DACDHJ010000000
VERSION     DACDHJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.070"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2007
                     /locus_tag="IV223_001392"
     CDS             <1..2007
                     /locus_tag="IV223_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8711690.1"
                     /translation="ARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQPVPVQ
                     ADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSALSQAP
                     YYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRNTSFG
                     NGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPASGNAG
                     GGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITMDQLI
                     QWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKYTVQS
                     GDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTGNTSG
                     SSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQQLTV
                     SNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGITMDQL
                     IEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGNTMYT
                     VKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVTGSKT
                     YTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2434..4848
                     /locus_tag="IV223_001393"
     CDS             2434..4848
                     /locus_tag="IV223_001393"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8711691.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5196..6749
                     /locus_tag="IV223_001394"
     CDS             5196..6749
                     /locus_tag="IV223_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8711692.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7130..8914)
                     /locus_tag="IV223_001395"
     CDS             complement(7130..8914)
                     /locus_tag="IV223_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711693.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8929..10650)
                     /locus_tag="IV223_001396"
     CDS             complement(8929..10650)
                     /locus_tag="IV223_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711694.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10750..>11453
                     /locus_tag="IV223_001397"
     CDS             10750..>11453
                     /locus_tag="IV223_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8711695.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNT"
BASE COUNT         3719 a         2128 c         2322 g         3284 t
ORIGIN      
        1 gcaagaaaag aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg
       61 gcagcaatcg tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg
      121 gttcctgttc aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca
      181 tttatcacag aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca
      241 tcagtgatga tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa
      301 gcaccttatt ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg
      361 gatacgcttg aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat
      421 ccttcttttg ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc
      481 aacggttatt actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca
      541 gcttgtttga cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc
      601 atcgcaactt acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga
      661 gcgactacag gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct
      721 ggtaatacaa actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct
      781 gtttggggaa tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac
      841 atccaaaaca atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat
      901 ggctcttcga cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca
      961 aacggaggac aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag
     1021 atctcaaatg atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac
     1081 aatttcgttt atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga
     1141 gcaaatactg gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct
     1201 tctaatacag cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc
     1261 tcaaataaat acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac
     1321 tttatctacc caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca
     1381 ggtaatacag gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt
     1441 tcgtctcaag gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa
     1501 aacggcatca cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat
     1561 ccaggacagc aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact
     1621 acttctgcag cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact
     1681 ggtaatacga tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat
     1741 catatcacga tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca
     1801 ggacagcaag tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact
     1861 ggttcgaaaa cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat
     1921 ggaattacaa tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca
     1981 ggtcaaacgt tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc
     2041 acaaaaatat ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt
     2101 aaaaagactg aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa
     2161 ctcgcctttg agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta
     2221 caacgttcga ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta
     2281 taaaaagagc aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa
     2341 agcaaaagga aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa
     2401 acaagtgttt tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag
     2461 aaaaaatggc aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa
     2521 aagccaaaat tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta
     2581 gggcatcctg aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga
     2641 tacaacgtgc tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg
     2701 ctagatacag gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc
     2761 caaatcaatt ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg
     2821 gagtattata aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat
     2881 gaagcagaag tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa
     2941 gtgatcgatg ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa
     3001 tggatgctga aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat
     3061 tggccagaaa gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat
     3121 attgaattta aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat
     3181 actctattcg gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt
     3241 acgacccctg aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca
     3301 gatctaaaga gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca
     3361 atcaatccag ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca
     3421 tacggcacag gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct
     3481 caaactttcg gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct
     3541 tacacagaag atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa
     3601 gcaatcgata aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc
     3661 tatcgcttac gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc
     3721 atccattggg aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg
     3781 cctaagacaa aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag
     3841 gagtgggtca atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca
     3901 atgccacaat gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa
     3961 aaagaactag cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt
     4021 ggagcagaac atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac
     4081 gatatcggtg ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc
     4141 ttaggcgaaa acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat
     4201 gtggtcgaaa cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta
     4261 gatgcttcga ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt
     4321 gtatggcgtt tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat
     4381 gatggcaagc ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa
     4441 aatcttcatt tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa
     4501 gtggacgctc tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct
     4561 cctcacatgg gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca
     4621 ccatggccaa catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa
     4681 gtaaacggta aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta
     4741 gaaaaagcag cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga
     4801 aaagttattg cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt
     4861 caataaggac tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc
     4921 tcagattttt tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat
     4981 gttctcaaac acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat
     5041 catcaaattt ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag
     5101 agtacatacc aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac
     5161 gcataatgaa tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg
     5221 aagtattatc agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa
     5281 tgttcaagct tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt
     5341 tcctatatcg ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata
     5401 atttgtatca cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc
     5461 ttctacttat ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc
     5521 aaagagcaca acagttttta cataataaac caacttctca agatgatgac tatctttatt
     5581 ttgagaagac ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc
     5641 tgagatcact tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga
     5701 acacattcca tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc
     5761 ctggtggata cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt
     5821 tagctggaac aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg
     5881 ctgcttctca catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag
     5941 ggttgttagc tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca
     6001 aacttgatac agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat
     6061 ttgtgccgat tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga
     6121 ttcctgaagg ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta
     6181 tctattcttc tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc
     6241 aaacgtctat tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg
     6301 gcaacgatgg tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca
     6361 tttacactac tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc
     6421 agccagtaca aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa
     6481 gtttgtttta tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg
     6541 aaaactatca gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa
     6601 gcgcgaatac gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga
     6661 aaatgtttgc ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc
     6721 aactatcgca agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca
     6781 ccgagtcaca agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc
     6841 gtgattcgga gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt
     6901 acaagatcgt gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg
     6961 gattgtgaca agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag
     7021 tatcattagt attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat
     7081 cgatcatgga ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct
     7141 gaaattgact attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg
     7201 ttcctttttc gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg
     7261 ttgacaaacg gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt
     7321 gctgaatcaa tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga
     7381 caggaggatt cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata
     7441 acgtgccgtt ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt
     7501 gatcacattg tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc
     7561 cgtaggcgat attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc
     7621 caaaagaacg acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga
     7681 tctttccttt attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg
     7741 caccagttgg accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat
     7801 tcatcaatat tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt
     7861 tcggttgttc taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata
     7921 tctcaaatat cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag
     7981 cggtagaaat tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga
     8041 tcgtcacacc acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga
     8101 gttgattaat gaaacgaatg gccggataga tagcacagtt cataaattga gcacgtttaa
     8161 aagcgttgta ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat
     8221 tgaatgtttt gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac
     8281 ccagttcata ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa
     8341 caattgaact gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa
     8401 acagcatcag accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg
     8461 tcaataacac ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta
     8521 cttgatgatt gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc
     8581 gcaccctcaa tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag
     8641 tgatgctgct taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt
     8701 tgatatcact gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat
     8761 cgatcccgat tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc
     8821 atagaaacaa tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca
     8881 aagcacgcgt gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg
     8941 tgattttgca aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg
     9001 tccaacgatt tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag
     9061 gcgttgagca acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa
     9121 tgctgcatct gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc
     9181 tggtttgatc aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga
     9241 accgccttga gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa
     9301 ttgagcgatc ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg
     9361 caagttgctt aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg
     9421 ggagcggatc tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc
     9481 tatcgtaggg tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt
     9541 cccgccaacg atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac
     9601 tggatcagag atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg
     9661 atcaaaggtt ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg
     9721 cagtatttct tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat
     9781 agctgcgatc atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac
     9841 accgatttgt aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata
     9901 aacgattccc atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt
     9961 gatcatttga ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg
    10021 attatcaaag gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat
    10081 ccgatccatt tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata
    10141 aacaatccat acataaaaaa caacagagat caatgtaacc catgttagta atagagaata
    10201 acgaaacatc atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca
    10261 aaataaaaca atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga
    10321 tccagaacta aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt
    10381 ctcccgcgtt tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac
    10441 tgcagcgatt gttcctagaa acgctacaag cagcatttca actccaattt tttttataac
    10501 tgaggcttcg ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc
    10561 taatgtaccg gcgatttcta aacagatcat cacaaaggta aggagagcga gtcctttgtt
    10621 tttctgtaag aatgttttta agatagacat attattcctc cttttttcct gatacttttc
    10681 gattctatac cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg
    10741 tgagaaaaaa tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca
    10801 aaatcaacga tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag
    10861 aatgattatc gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat
    10921 agaaaattag atataccgat caaacagatg aagaatttat atggaaaaac attgacggag
    10981 ttatatgaga cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa
    11041 caaaaacaaa aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac
    11101 gaggaagaat ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa
    11161 gatattgtgg aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg
    11221 ccagcttcca gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta
    11281 tttactcaag acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg
    11341 aaatccgaga tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat
    11401 gaaaaaggat ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tac
//
LOCUS       DACDHJ010000069        11308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDHJ010000069 DACDHJ010000000
VERSION     DACDHJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.071"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(89..1552)
                     /locus_tag="IV223_001398"
     CDS             complement(89..1552)
                     /locus_tag="IV223_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8711696.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1616..4633)
                     /locus_tag="IV223_001399"
     CDS             complement(1616..4633)
                     /locus_tag="IV223_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8711697.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4784..5623)
                     /locus_tag="IV223_001400"
     CDS             complement(4784..5623)
                     /locus_tag="IV223_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8711698.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5768..6013
                     /locus_tag="IV223_001401"
     CDS             5768..6013
                     /locus_tag="IV223_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711699.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6076..6498
                     /locus_tag="IV223_001402"
     CDS             6076..6498
                     /locus_tag="IV223_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711700.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6592..6909)
                     /locus_tag="IV223_001403"
     CDS             complement(6592..6909)
                     /locus_tag="IV223_001403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711701.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6936..7142)
                     /locus_tag="IV223_001404"
     CDS             complement(6936..7142)
                     /locus_tag="IV223_001404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711702.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7123..7305)
                     /locus_tag="IV223_001405"
     CDS             complement(7123..7305)
                     /locus_tag="IV223_001405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711703.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7319..7669)
                     /locus_tag="IV223_001406"
     CDS             complement(7319..7669)
                     /locus_tag="IV223_001406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711704.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7695..7997)
                     /locus_tag="IV223_001407"
     CDS             complement(7695..7997)
                     /locus_tag="IV223_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711705.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8000..8233)
                     /locus_tag="IV223_001408"
     CDS             complement(8000..8233)
                     /locus_tag="IV223_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711706.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9219..9541)
                     /locus_tag="IV223_001409"
                     /pseudo
     CDS             complement(9219..9541)
                     /locus_tag="IV223_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9664..10779)
                     /locus_tag="IV223_001410"
     CDS             complement(9664..10779)
                     /locus_tag="IV223_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711707.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10785..11183)
                     /locus_tag="IV223_001411"
     CDS             complement(10785..11183)
                     /locus_tag="IV223_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711708.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         2971 a         2336 c         1744 g         4257 t
ORIGIN      
        1 aaacgaaaat gagagataca aatatatcaa atatttaaaa agtaaccaca ttttctatac
       61 cactccttat cagaaacagc cttattcttt agaataagac tgtttctggg ttgattaatt
      121 tgtcttttgt taaactacca tctaaggcaa gttctaaatg taaatgtggt cctgtactat
      181 ttcctgttga acccacataa ccaataattt gtccttgttc aactgtttgt cctacgttca
      241 cgcaatattc ctgttggtgc gcataaagag ccacacttcc gtcttcatgc tcaattgtga
      301 cataatttcc tgacaaagta tgatacgaag cttctatgac cgttcctgcc ttgctcgcat
      361 agattggttc tccttgattg gctgccatgt ctaatcctct atgaaattct tctcctcgcc
      421 aaccgaagga actagaaatt gaataatttt ttagaggtac catatagcct tctttgctta
      481 gttgtggacc agctacttct ttatcatatt tgattaagtc ataggtttcg attaacccat
      541 ttaatttttt atcgtagcta gtgtctgttg cgtatcttcc tgttaaaaat ttagtcgctt
      601 cctgataggt ttttgcctta cttttccaaa caccatcata aaaagaggta tttccaacta
      661 agccttcttt taacagtttg gcatagtctt ttaagctttc ttcatagctc tcatattgcc
      721 gaaaagttgc ttgggtcgta taaagtgtcc cattgcctaa atcttcttgt gtcgcaaaag
      781 agacgccctt tccgttatgt gtgcctttga ttccaaatag attgtagttt ggtgcctgag
      841 ccaattgact ttgtccgctt gcggattcta aaatggcttg cgcgatcata acagaagcgt
      901 ataaatcatt ttcttgtcca attttacgag cagattcacc gattttacgg ataaacgttt
      961 ctacagacgc gtctttttca aaatgaatag tcccttcgtt atcttgtgtg ctggaaggga
     1021 ctaattcttg tatcggtgcg cttggtgctt ggtggtagtt ttttgtctct tctgaaccga
     1081 ttgattgtgc ctgcggtacg tcattcatag gtacagtcac actctgttca ggtgcctttt
     1141 gtgggcgttc cgtttttgtt ggcggttccg cttctgtttc ggtacttggt ttttgaggag
     1201 tctgtgagct ttcatcagaa ggttgctcat ttggttcttc tgacggttcc tcctcaggtt
     1261 gttgattgtc ttctggtttg tctggttgat tcggtgtttc tggttgttct tcagaacctg
     1321 ttggtggagc gttcatttct ggattatctg gtgtatctat tgtatcttca gtcggtgttt
     1381 ctggttgatt ttcttctcca gtggaatttg ttgtcgtttc ttcagtgatc gttgcagatt
     1441 gcgaatttgt cagttgattg ttgccttgtt cattagccaa aaccgctggc gtatacccaa
     1501 atacttgacc tactaataac aaagcagata aacatttact cgtttttttc acaagattgc
     1561 ctccattctt caatcaatca tctagagaca gctgattgct gtctctagtc atactttaat
     1621 tgctttgttt cattttacga cgaaccagaa cagcccccac accaacaaaa agaaacagtc
     1681 ccaatgcaat taacacatcg ttcactgttt ctcctgtttt tggtaagctt tgttttggtg
     1741 tgctcgtcgg cgtttgtttg acaggggttg tcggcgtttc ttttttaatg ccttttgctg
     1801 tcaatgtttg tttttcattc ttcaaatcaa agtgacgtgc ttcttcctct ttatggtctt
     1861 tatccttata gacaatatga gttgcgacca atttctcccc gtcttttaac gtgcctgctt
     1921 tgtagtcaaa ggatacttta aactcttcag acgctttaga aactttacgt gcagtttcta
     1981 ccgtaccaac gactgtatct tttccatttt tatcaatctt atggaattga gtcacccagt
     2041 atttggtttc ccctaccgta gattctggaa attcttgcga tacatgatcg atcaactctt
     2101 ggtctttgtc tggatcaaat gtttggtcgc cattcactgt aataaagcgt gtttcgattt
     2161 ttggttcttt cgcagtcact gtttgatctt tattggttaa atcaaaatga cgagcgaatt
     2221 cttcttcctc ttctttgtcg ttatagacaa tgtgcgttgc gacaatcgtg tcaccgtctg
     2281 aaaccatgtc tgctttgtag ttgaacgtca catcaaatgt ttctttttca tcggtcactt
     2341 ttcgtgtttc ttttaccgta tctaatacag tgacttttcc atcggcagta attttatgga
     2401 attgcgtcac ccaatatttc tctgtcccaa ccgcagattt agggaatact tgggacactt
     2461 tatccactaa ttcttggtct tttttcgggt tgaaatcttt tccacctgtc gttgtcacaa
     2521 acaaagtaga aatttctggt tggtcttctt ctggaaccac gatttcttcg tttttcacag
     2581 taattggttg tacttctccg tctttgaccg ttactaaaat cgtttctgtt tgtcctgacg
     2641 cttgcccata atgctcaggg aacacaattt ccgtaaattt atacgtaccg tctggtaatt
     2701 ttagtttttc tggttcttct tttgtttcat acacaaagga cacattttta ttatgtgcag
     2761 tcataccttc aatcaggaat ttcgctcctg gtaattgtag atgattgatt gaatcgactt
     2821 tattgaagtc tacttcaccc gtttttaaca agttgtcaat cacatctaat tcaaacgtaa
     2881 gcacaggcgt cgtgttatcg ccataggcca cagagaacgt ggtttgttct tcagaaggtt
     2941 gacggtagtt gtctggcaca tttttttcaa tcaattggta tttgttttga tcgccattgt
     3001 agaatttttt gtttgccttc cagtgtccgt ctttgtcagt ttttactgaa tccaagactt
     3061 ctcctttttt cacaaggact gtttctttat ctggcgcagt aatatctgtt ttggcgataa
     3121 tatcaaattc gatattttct gcaggaacgg tatcataaac aggtttgcct tgattccagg
     3181 attcttccgc attatagact tctttgactt tctttccttc tggaatgaac tgttgtaagt
     3241 cattttttgc agttaacgtc acttctttgt ttggttcaat cgtaatcgta tgagtttgtt
     3301 tgtctagaat atacccagct ggtgctttga cttctttgac atccaattta ttttgaacat
     3361 attttccgtc tttttcatag aatgcaggga tttcttttaa ggcgatttcc cctttggcat
     3421 ccgttgtata ttgttttgtt tctcccgtat catgacgttt gatttcatac gtcacatttg
     3481 gaactttttt ccctgtgaca gcgtctagtt tggttagttt gaagtcgcca ttttttagaa
     3541 ctttaatagt tagtccagct ctattaggat ctggcacata aagctttcct agattttgag
     3601 caacaggatc tacataggca aaacttgaac ctacccctct agaatttgga tccagtgaag
     3661 aaacagaaat tcgtccgtca tttgagtttt gatttgcttt tataaacaaa ctatttcctg
     3721 attttcttac ttgaacgtta gctgtgtttt caacaaccga cccatcccat tgagaaaaca
     3781 caccttttgt atccttaact tctacttctt cccctacttt aaccgtcact gtttggctat
     3841 caaaagaagg atcttcaagt aaattgttta tttttctatc tacagtctgt ttgaaagctt
     3901 cataattaga ttgtccaata gttccataat taacaaatga accacctaaa acttcccaaa
     3961 tatatgtttg tgctaataca tagttatcca ttgaaccact agaatagtag ccaaaataac
     4021 caattaaaga aatctttttt ctaatagcac ttgtcgtaat taaattttcg agtggtgtca
     4081 catcatattg aatacccgca ctcgtgattg cgccttcttg aatgcaaaaa acatcttctc
     4141 cgtcaatttg ccagcgaacc atgtaatcat gagtatacat tgttccttca ggagtaattc
     4201 gagtcatatt cacatcttta cgaccatttt cagtcaatgt tagattggta gcacgtgtct
     4261 gtgcattctt ttccaagatt gcttgccatt cttcagattc ttgcgccaat cgttcttgtt
     4321 cagaaaaaga agtcgctttt tgaatatctt caatggttat aaagtatttc tcataacctt
     4381 cagggacctc ttgagtcatt acattgggcg gagaaacatc ttctgtcgtg tcctgcgtag
     4441 ttgtctcttc tgttgtttca cttgtggttt cagtactgat cgcttcttca ctactttttg
     4501 attcttcagt cttcattact tcttcagtag aatttgattc atttatcgtt tctgcaaatg
     4561 ccgttacaga tcctaacatg ggattgagaa atgtaacgaa aattgccaac caagttgcca
     4621 ttcttttaaa catatttttt tcgttccttt cattttattt ttcagtcttc taaagtaatt
     4681 tcttatggac ttcgacaccg aaacattttt atcctcctca aaacaaaaaa agactagaac
     4741 ttataattct agtcttcatc aaccacttac ttttctttta tttttagtag aaatctacag
     4801 tgtgtttttg tgacccgtca ctgtaaataa taacatccac cataaaacct ttgttgtacc
     4861 actgcgaatt tggatcgcta acctctgcat tagcccaatc ataggcttca tctacagtgt
     4921 taaatacctt tccactatta ccaatctctc caactacaat tgttggctct tcaggtgtcg
     4981 ttgtgccacc atttccgcct tgatcgcctc caccaggatt gcttggttct tcaggcgttg
     5041 ttgttcctcc acctgtgctt gggttagaag gagttgtttc tcctccagta tttccaccat
     5101 tacctggttg tgtaggtgtt tgagtagtgc caccattgtt ttctggtgta cttggcgttg
     5161 ttgtaccacc agttgttcct gtttgatctc cagctggtgt tgttggtgtc gttgtatttg
     5221 gtgtcgtttg tgtatttgaa gtcgttcctt gtcctactgg ttgattcgga tcaactttgt
     5281 ctttctcttg aatcactgtt tgagccacag tttcacctgc gctgttttgt acacttgcta
     5341 cattgccatt aaatactaat ttatttcctg catagattaa atccacattt gtaatcttat
     5401 tcatgtccgc taatttttgg acagtaatat ttgttgcttg actgattgcg cttagggtat
     5461 ctccccagac aattgtgtat tcattccctt taatttctgc tttgatttgt tccgccgaac
     5521 gaggtgtcca aactttgttt tctgctgctt cagcattcgt tcccacagca agactaactc
     5581 ctccgactac gattcctagt gccaatactt tcaacatttt cattgttttt cgcctccgat
     5641 ttgaataaat ttatttacaa aaacatagta ccaaacttat gtctatcagt cgatatattt
     5701 tttgtagatt tttttatttt ttgatatact aactttggta ctaaaataac taaaaggaat
     5761 gtcacacatg caacttaata atttaaatga aatacatgat tgggctcaaa ataatttact
     5821 taatcgtaaa caagctgcca aaatcactgg acaaaactat gatgcttttt ctagctcttt
     5881 acgtagaaat aatatcaaac catttctaga aataggcgac gaacctggtc cttccatcat
     5941 tcgcttatat ctaagagaag atattgaaaa atacgcaaaa cagctcgaag aaaaaagaca
     6001 gaaacgtcaa tgaggcgttt tctgtcttgg atttacaagt aaaacaataa taaacacttt
     6061 tacaacggag gttctatgaa taagaaatac attgtttcag ttggttcaat cgtactgatt
     6121 gtactcgcta ttattgcttt ttctgtatcc aacaaaacag aaacacagcc acaaaatacg
     6181 gttcaatcga ctactacaag tcaatctagc gatcattccg tctatccttc actaaatagt
     6241 ttaaaagaaa cgcatggaca agatttaatt gatcttggtt cttctcaaat tactactgtc
     6301 ttatttgaaa agaaaaatat tgcgtttatt actcctaaag aaggaactga ccttgaattt
     6361 tctatcaaag taacaaatac caaagaaaat ttattctcca ttcctgacat tcaatatacc
     6421 cctaacaagt taaagattgg ggatacgttt ggtttggcta aagtaaatga acaatacttt
     6481 gcttactcac tgaaataagt ttctaatagc aagaggaaaa accctcttgt tatttttatc
     6541 acataatcat tttataaaaa cgcttcgaaa atatcaacat ttttgaagaa actatttttc
     6601 taatttctca atttcttcca agggattttc gctaatgacc catacaggca cagattgctc
     6661 tcgaggctcc ccttcttcta accaccaacc tgcataatga acttcttcct cctcagcatt
     6721 accatgatac caaccaacaa aggattcatc tacaacgaat gaaatttctt cttctggatc
     6781 aaataattca tttttccatg cttcaattgc ttgttctttg gtgtactttt gtttgttaaa
     6841 tacaccacaa atttcgtcat ctacataaaa aatttcaaaa tcaaaattac tcgttccttt
     6901 tactggcatc ttaacttttc ctcctttgac tcacttcaat agatatacca ctcatgttca
     6961 ttccattgtt tactagttaa ttggagtttt atcttagggc tttgtgcact ctttccaaaa
     7021 acttgagcgt cgtttttttc ttctaaccaa aaaaggtaat ttcttacatt cggtactaaa
     7081 tgtttttcct gcatttcttc atgaaaaaca tataattctg cttcattttt caaccaattc
     7141 attattcatt ccctcattca cttcttcata ccgccattca gatagtgaga tttctttttg
     7201 tttctccact tgtcctataa aaatcgctgt attcaacaag ctgatcacac ttaataataa
     7261 agcaatgaaa ctaatggtta cacttactct ttcacatttt gtcattttgt ttccttcttt
     7321 actcgtttgt tatttcttgt aataggtcta ataactcctt caccgaaatt tgcaaatggt
     7381 tcgcaaaaat ttcaataaaa gcactcgcac acattaccag accttcaact gatatatcac
     7441 tgcgacaata aaattctgga gaaacactga tttctgattc tttcttacaa ttgaacgaaa
     7501 aaactatttt tgaatcaaat tccggtaatt cttccacaac tatgttttgg gattctacca
     7561 agaaagggag tgcctgttct aatgcttctt ctggtgtttg agcagataga ataattgctt
     7621 cttgttcttt tatttcattg ttctcaaata ctttataact ttttttcatt gttagttcct
     7681 ctttcattcc tctctcaatt agctaagccg ttactggtac gccattctct aaatttagta
     7741 tcgctaatcc ttaatgctcg tcgaatatca atcattcgat aacctattgc acgaagttct
     7801 ttatatcttt tagcagttag cgtctctaac gtcatttcag gcaatgggtt cgaatcaaaa
     7861 atagaattcg aaccgattat ttgggccaat tgttgtactt gtaacagctc ttctgaactt
     7921 aattcagtga ttgctcgatt gcctatcatt gcatacattt tctgtcgaat atacttcttt
     7981 tcttgtttag acaacatttc tatccactcc tattttcatt atcatttgtt acctccactt
     8041 cagtaaatcc acaaataaat gaattcatat tccaagtacc attcccttca actacaactt
     8101 ttctcctgtc ctctttcgga tattcaagaa tcagaccgtt taccaatatg tttttcacta
     8161 ctactaattt gtttgtatac cgtggaattg ctgttccgat atacttcact ttatttccta
     8221 cgctaattgt catttcctca ctcctattct tttttatcca aaactttttc ctgtcctatt
     8281 cctgccaacg aatatacttc actgcatgct taaacaaaac aaaatgttgc cctgttttat
     8341 ttccgtcttt tacttgttcc acatacagtt catatttggt cacacctttt aaaacgccag
     8401 atactttttt tcatccattg tcacaattag ttttttcatc aatcgattga tactgcactg
     8461 ctcatttttc ttgttcatat agtattcctt tctttttcaa aactagcctc ttttgtttac
     8521 tagttttcat caatcatcaa cgatttaaca gattttttct ttcgcagggt ttctagcgtc
     8581 agcaacccca aactctaaaa aagtgccttt gctcccttga aaaacgagcg gacgattgta
     8641 ctcgtcatca tatgtttttc atactacctg atccacgcaa ataacctttt tttgttctgt
     8701 gccacaccca tccatacgta tcgcttgaga ttcaccatac ttttttaggt ggtttatcct
     8761 tcctaagtcc ctgttagaca cgctgattaa cttagaaagt cctgtttgta tccaattttc
     8821 aaagatctcc caaaagaaat aagtaggttt acgtgtatcg cttctgttca aagtacctgt
     8881 ttgtacttga gtatttctac tcccctcgtt caagggattc cttccaccag ctagcttttc
     8941 ctttcatcat caaatgttcc tgaaaagcat ttacgtttcg tgtccgtgtg cttacttatt
     9001 cttttttcga tagcgattat ccgctcgcta ctcgctattt tatactttgt ttgttagctc
     9061 aaataaaaac taacaaacaa aaaaggctag tttctttccg ttttattttt ttaactgtcg
     9121 ataaatagtt gttagtttaa ttccaagttc taaaaccaat acgcccaaca aaaccacagg
     9181 aaaaacaatt ggggcaaaca agtaaaatcg gatagttttc atgtcttccc tctttctttt
     9241 tggtgtcgct gtggtcgttc ttgccgttca atacttcgat acatttttta ttcattattt
     9301 gctcgttttg ttttcctctg gactgctttt cttgcttttt gaatacttta tacgcttttc
     9361 gtgcctgaat taataggtct tgtcggcgtt tcattcgtga gaaaacaata tttgaaacca
     9421 tcaaatcctg gttcgaacaa ccacgattcg caataatttt cccatcaaaa aaaatagttt
     9481 tcacttgaga aaaaaacagt tggaaatctt gttttgttgc taattttttt gtcaaaatca
     9541 ctgtgctttt ccctgttgaa tctacaatga ttcttcgaac aatccgattc gataacttcc
     9601 aacgttgccg attaaacaat gttacttcac aacaaatatg acgtaagtga ttaggggtca
     9661 ttttcatgtg tccaattcct ccaaccaatt atgcatcgaa attttgggca attgtgacag
     9721 cccataaatc tgcgtttcca ggtcttcata actacaagaa cgcttggctt gaagtaatga
     9781 atattctgct tgatctgata gttttgttgc cattggatct agattcaatt ctcttacaca
     9841 acattcccaa aaattcaata gcgctctaaa ataaaatcct tttcgattct gaattgcttt
     9901 tccttcatga cgagcaattt tttcattttg aatacaccgt tgatacgttt tgcttaaatc
     9961 ttctgcaaat gtttccccta aaattcgata ctcgcttcca tcagataaac catgtgtttg
    10021 attatacttt cgttgccgct tttctataat tcgtttactg tcgtatatca tgtttagctg
    10081 ctctttcgct tcacgccatt cagctgaact gcgaacaatc catgtcaaaa catctttcgg
    10141 aacaaacgta ctttcgctgt aatgagcaat aaactctttt tgccaatcat cagccatttc
    10201 aacaggacta gtcgtatcga tttttgtatc tagattttct gtatctagat tgttagaaga
    10261 attattaaat aattgtgcag aattttcaca tggatttttt tcgtcaaaat catcgaaaat
    10321 cgctaaatta tcaacagatt cctcctcttc ttttccttgt gcagaatcta cactggttga
    10381 gtcaacagga cctaatgaat acacatcatt ttctgtcgta tctaattctc ctagatacaa
    10441 ccgagctttg tttctttttc ctgtttgttg atcaaatccc atatcctcta tttctagtag
    10501 ctcatattct ttcaattctt ttttgattgt attaacagaa cgtctgcaac aattcaatgc
    10561 attggctaat tcttgttccg taaattcaaa ataaacaaaa ccttgactat ctacttgatt
    10621 tcgttgaatg gctaaatcta agcgtgaccg aaaaaccata tacgcgattt ttgctttatc
    10681 agacaatgag cgatattttt cacttgtaat aaataccttt ggaaattgat agtataattc
    10741 attgtattta cgagttgcgg taaatctttt tgttgccatc gttattacac ctcatcttgt
    10801 ccatatatta agaaagaatt ttggtgttgg tagacacgaa catcttccat tgtagcaatc
    10861 aattctttta gataaattcg accgaattca attgttagta agtcgtaatc ttctaattca
    10921 tttttgattt cttcaatttc ttcttcagac acattgagta aaatggctaa ttcttgattg
    10981 gtataagaaa tatagacatg tttcttttca tcaatccgtt tttcaattaa ggattcttct
    11041 aagtactcat tcagtaagct ataggcgtat tttgcttgtg cagtcatcgc ataatatttt
    11101 gacgatttta taaagaatag ttttggcata tgaaaagttg cttgtgtcag cacatctgtt
    11161 gcgcgaaatt tgttttttac catatttttt cctcctagaa aaacacgcaa aaaaagctct
    11221 gtataaatcg aattacacag agtttcttct tgttgatatt taatttctaa aatacgtttc
    11281 gggtaaaaac taaacactta ctaatatt
//
LOCUS       DACDHJ010000070        11281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDHJ010000070 DACDHJ010000000
VERSION     DACDHJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.072"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(84..2093)
                     /gene="metG"
                     /locus_tag="IV223_001412"
     CDS             complement(84..2093)
                     /gene="metG"
                     /locus_tag="IV223_001412"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8711709.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2361..2639
                     /locus_tag="IV223_001413"
     CDS             2361..2639
                     /locus_tag="IV223_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711710.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2736..3557
                     /locus_tag="IV223_001414"
     CDS             2736..3557
                     /locus_tag="IV223_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8711711.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3842..4852)
                     /locus_tag="IV223_001415"
     CDS             complement(3842..4852)
                     /locus_tag="IV223_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8711712.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4864..5817)
                     /locus_tag="IV223_001416"
     CDS             complement(4864..5817)
                     /locus_tag="IV223_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8711713.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5807..6877)
                     /locus_tag="IV223_001417"
     CDS             complement(5807..6877)
                     /locus_tag="IV223_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711714.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6883..7926)
                     /locus_tag="IV223_001418"
     CDS             complement(6883..7926)
                     /locus_tag="IV223_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711715.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7927..8868)
                     /locus_tag="IV223_001419"
     CDS             complement(7927..8868)
                     /locus_tag="IV223_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711716.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8952..9299
                     /locus_tag="IV223_001420"
     CDS             8952..9299
                     /locus_tag="IV223_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8711717.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9369..11030)
                     /locus_tag="IV223_001421"
     CDS             complement(9369..11030)
                     /locus_tag="IV223_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8711718.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3182 a         2292 c         2056 g         3751 t
ORIGIN      
        1 taagaaggaa tttacgaaaa ttgcttctca aatttcggaa aaagggggta tgacacaact
       61 agtgtcatac cccctttgaa tttctatgcg atttctgagc catttggcat ggattttggt
      121 gcttcgatta cttgtaaaga gccatctggt gcttctgcag aaaggatcat tccttgactg
      181 atttgtccac gcatctttcg tggtttcaga tttgctacaa tgacgacttt tttcccgatc
      241 aattcgcttg gatcaggata aaattcagcg attcctgaca agatttgacg gtcttggctg
      301 tctccagcat ctagtcggaa ttgcagcaac ttatctgccc cttctacttt ttgacagttg
      361 atgacttctg ctacttttag ttctactttt tcaaatacat caaatttgat ttgtttttct
      421 ttcgttgaaa caagttctgt ttcttctgga tcccatttga ctgtatcttc gtttgtttga
      481 gtaccttcag acattttttc ttgaataaaa gcaacttctt cttccatatc taaacgtggg
      541 aagattggtg taccttttgc aactactttt gttcctgaag gaaattcgcc aaagtgtaat
      601 ccttcaagat tcattgtttc agagtccaat cctagttgat tgaagatctt ctctggcgtt
      661 tctgtcatga ttggctgaag taatatcgct gcgatacgca aagattcagc taaatggatc
      721 atgacactat tcaattcagc tttcttttct tcgtcttttg ctaataccca tggttctgtt
      781 tcatcaatgt atttgtttgc acgagaaatc agcacccaga tttcagccaa tgctgtattg
      841 aattccattt tttccattgc atcatggtaa cgtccgacaa cgttagcagc cgtcgtagac
      901 agttcactat caaatggagt gacaagtgag gcatagtcag gaacgagtcc atcacaatat
      961 ttgttgatca tagcaatcgt tcgattcaat aagttcccta gatcatttgc cagatcataa
     1021 ttgacacggg aaacaaaatc ttctggcgta aagacaccat ctgacccaaa cggaacagca
     1081 cgaagcaaat aataacggag tgcatctaaa ccataacgtt ccacaagcat ttcaggataa
     1141 acgacatttc cttttgattt agacattttt ccgtctttca tcaatagcca gccatgccca
     1201 aatacttttt taggtagagg caaatcaagg gccatcagca tgattggcca atagatcgta
     1261 tgaaagcgaa cgatttcttt accgaccatc tgaacatctg caggccagta tttttcgaat
     1321 agactttcat cttcggagcc atatcctagt gcagtaatat aattggataa tgcatcgatc
     1381 catacataca ccacatgttt cggatcactc ttgactggta ctccccaagt aaaagttgtt
     1441 cgtgaaactg caagatcctc taatcctggt ttgataaagt tgttgatcat ttcgttttta
     1501 cgagattctg gctggatgaa ttcgggatgt tcttcatagt attccaatag acggtctgcg
     1561 tatttgctca tgcggaagaa gtatgattct tcttttacca attctacttc gtgtccactt
     1621 ggcgctttac caccgatcac gttaccttcg tcatctttgt acacttctgc aagttgtgtt
     1681 tctgtaaaga attcttcgtc agaaacagag taccagcctt catattcacc taagtaaata
     1741 tctccctgtt ctaataaacg atcaaatatt ttttgaacag ctgctttatg gtaatcatct
     1801 gtcgtgcgaa tgaatttatc ataactgata tcaagcgttt tccataattt cttgacatca
     1861 gccgccattt tatctacata ttcttgcggt tttacaccta gttcttctgc ttttgtttcg
     1921 attttttgac catgttcatc cactccagtg agataaaaca catcaaagcc catcaaccgt
     1981 ttataacgag caattgcgtc acatgcaatc gtagtatacg agttgccgat atgaagtttg
     2041 ccgcttggat aatagattgg cgtagtgata taaaaagttt ccttttctgc cacggggctc
     2101 ttcctccttt atcgcatgat gcgaattcgt ttccttgcta gtataccata aaaaaagcat
     2161 gctgttagct ttttttcaaa atgaaaaaga ctggataaac gacttctatc tacgttcaat
     2221 ctatccttta aaaggatgaa taggttgaat aaatgcgggt ttttgttttg tttcatatct
     2281 actaaaaaac ggctttttat ttctcccctc acacgtggta taataaagac tatctatctt
     2341 agtaagggac tggaacaatc atgcagaaga aacatctttt ttttacactt agtattgctt
     2401 ttctttcatt agcacattta atattcagct atttttatat tcgtatgtat ggttatttta
     2461 acctccatgg gcatttgaac agttttatga cagcagcttg gattttgcgt tttatcattg
     2521 acgtatatat cgttatttgt ggcttctttg ccattcgtga agagcggtat aaagtattgc
     2581 cgttttatct gcttttcttt ttattcaatc tcatacttcc atttattttt catatttaaa
     2641 tgataaccta gatttttcaa gtaaatcctt ggtttttttt cagagtctgc tacaataagg
     2701 taaaaagtac ttttactgtc aatggaggtg gtcagatgaa ccgttttgct tctaaagaag
     2761 aagaaaaatt atattatgaa catgaagcaa gtgaagaaga atttcttaac tggtatcaaa
     2821 aacaggaacg tccagaatat gacaagccct ctttgactgt cgatatcgta ctgatgtgct
     2881 ataacaaaga agaagatcag ctgaaagttt tgttgatcca aagaaaaggg catccttatc
     2941 gaaattcatg ggcattgcct ggtggctttg tccaaaagga tgagtcaact ggagaaagcg
     3001 tcttaagaga aacaaaggaa gaaactggtg tggttatttc aaaagaaaat attgagcaac
     3061 tgcatacatt cagtacgcct aatcgagatc cgcgaggctg ggtcgtcaca gtcagctact
     3121 tagcctttat tggagaagaa ccgctaatcg ccggagatga tgccaaggaa gtacgctggt
     3181 tcacactaga acgtcatagt aacaagatcc atttatctag cggagaagtg gctatctcgc
     3241 ttgatttagt cactggagag tcttctggca aagatacttt ggcttttgac catagtcaaa
     3301 tcatcttgaa agcattcaac cgtgtagtca ataaaatgga acacgagccg caagttctac
     3361 aagttttagg taaagatttc acgattacgg aagctcgaaa agtatttgct aaattcttag
     3421 ggatcgatta taaaacgatc gatcattcca attttaagaa agctatgctc caatatttcg
     3481 atgagatcgg tgaacgtcct gtaggaatcg gccgtccttc aaaaatctat cagctcaagc
     3541 cggggcacca tgaataaggt tgtgagcaaa agattcagac ctaatcattc aagaaaaaac
     3601 gcaaaaacaa ctctttgtga gttacgtttt ttgtttcaga attttgatgt attcgtaaac
     3661 tttctgaata aaaaattaac aatagcagaa aaagctcgta cgaacttttt ctgctattgt
     3721 tttattggtt tgtcataatt agtgagcttt tcttcaaaag aaaaattcct agaaacaaaa
     3781 gtcattaaga ctttttatta acaaggctaa ccctaacgct cctatcagtt tcattcattt
     3841 tttatccctt gataaccgaa tgtctgccca ttttttctac tacttggctc tttcttacgg
     3901 aattgctttc ttctgctccg tcggtattga tcaaaagaaa ttttttatta taccattggg
     3961 acttataacg caattttgtt attaatttat gccagtcgtg aatagaaaaa tcagcatgtg
     4021 cctcttcttt tggaaaagat tctacaaata agtagtcata ttgtttgacc aatttcccag
     4081 ataattgttc aagataatct tctcgctggt tcttttgttt cagataaaga tccattactt
     4141 ttctcttttg tttctgataa ttgcttgcct gatctaacct gatctttcta tggtgagcgg
     4201 agcggacttt cacattcaat ttccgctgtg catgttgcag cttttcttct gtctggtgaa
     4261 attttggtaa agttacttct agaggtcgag atgtttggat caatgtcttc ggatgatagg
     4321 cgatcccgat ccatttagct aaagattctt tttttactgg cgaaacatca caaagtagtg
     4381 cgacataaaa ttcttcatta tatcgagcag aaatcgtagc ggagcgaatc gatccttcaa
     4441 ctggccgatg ctgatttatc ttgattagtt ctttttgttt tggtaacttt aagtagccat
     4501 tttccagata gatcgtacgg gtttggttgt tggtcgtata agattgccac atactttttt
     4561 tcgttttaag ctttgggaag ctggcacgtc ccttgaaata attgcgaaaa gcccgatcca
     4621 aattaagctg tgcatttgct aaagcaagac tgtctacttc ttttaagaat gggtattctt
     4681 tttttaatgt agcgggtgtg agcgaaaaat tggctactcc tgtttgttgc caacttttct
     4741 gccgcttttt cagcaaatgg ttataggtaa atcgagcaca tccaaaagta cgaatcaatt
     4801 gctgtttttg ttcttctgtc ggatacatac ggaatttaaa agcttttagc atctatgtcc
     4861 ctcctagtta gcaaccagaa gagattcctt ttgtcgtttt agtttttttg cgtaatatgg
     4921 gacttcctgc tcagttgcat agacaaaatg ttctggtgcg atttcacgaa gttgacgtac
     4981 ttgtccgtca tctggttcag gttgatattt gattcttctg cgttttctct catggtctgg
     5041 atctggtaga ggaatagcag ataacaaact ttctgtataa ggatgaacac cgtgattata
     5101 gatttcatca cttgttccca tctctaataa caagccgtta tgcattacac cgattcggtc
     5161 actaatatgt ttaaccatcg acaaatcatg tgcaataaat agataggtca attgatgttc
     5221 tttttggaga tcttgcaaca aattgaccac ttgggcttgg atcgacacat ctagtgcaga
     5281 aattggttca tcacaaatga taaaacgcgg ttttactgcc aatgctcgtg caataccaat
     5341 acgctggcgt tgcccacctg aaaattcatg aggataacga gtaccgtggc ttggattcaa
     5401 tccaacagtt ctcaatagat catcgacttt ttttgcacgc tcttcagatg atttagctag
     5461 tccattaaca tcaattcctt cggcaatgat atctctgact ttcatacgag gattcaacga
     5521 agcgtatgga tcttgaaaga tcatctgaac atcatgccgg aatttcgtca aacctttttt
     5581 atctttgatc ttcgttatat cttgaccgtc gaataatatc tcaccatcag taggttcatt
     5641 caagcgaatg atcgtacggc cagtagtgga ttttccgctt ccagattctc caactaaacc
     5701 gaatgtttct ccttcataaa tgtgaaagct gatgtcattt acagcatgta cttcattctt
     5761 ttttcctaca ttgaaatact gtttcaatcc tttaacttca agtagtttat ttggcatcag
     5821 caattcctcc ttgaccgtaa gtatgttttt gtttctcttt aaagatagcc cagcgacgtt
     5881 ggatttcttc tggcggtgtc actttgggtg cctgcggagc aagtagccaa gttgctgcct
     5941 tgtgtgtttt tgatagttca aaaaatggtg gttcttgttc tgcatcgatt ttcatcgcga
     6001 attcatttct tggatagaat gcgtctcctt ttggcgggtt caataaatcc ggtggagaac
     6061 caggaatagc atacaatcga tcgttcgcac tatctaatgt cggcattgaa ccaagcagtc
     6121 cccaagtata gggatgttgc gggttgtaga aaatttcttc cgatgttcca acttcaacga
     6181 ttcttcctcc atacatgaca gcgacacgat cagcgacatt cgctactaca ccaaggtcat
     6241 gagtgataaa gataattgat gtatcgattt ttgtctgaat ttctttcagc agctccaaaa
     6301 tctgtgcttg aatagtcaca tcaagcgctg ttgtaggttc atcagcaatc aggatttccg
     6361 gataacatac cagtgcaatc gcgatcacga tccgttgacg ctgacctcct gagaactgat
     6421 gcggataact gttgatacgt ttctcagctt cgggtattcc aactaattta agcaattcta
     6481 atgcagcttt ttgtccctct ttttttgaaa ctttattatg ctttctaagg gactcagcga
     6541 cttgcttccc aatgggcatc gtcggatcta aagaggtcat tggatcttgg aaaatcatcg
     6601 ctatttcttt accacggatt ttttgcatct ctctttctga ttttccaacg atatcgttac
     6661 ctttaaagag gatttgacca gcatctatat tggcgttgct tgacagcaag cgcatgatgc
     6721 ttctggtcgt cactgatttt ccacttccag attccccaac gatcgccaat gtttctcctt
     6781 tattcaaatg aaaattgaca ccgcgaatcg ctcgtacctt tccagcatac gtatcaaaag
     6841 agatttctaa gtctttcact tctaaaattt tcgtcatcgt cttcactctt tcattttagg
     6901 atcaaaggca tcacgcaatc catcagcaag taagttaaag ccaatcatga taaccgataa
     6961 agttactgcc ggaatccata atagataagg cagatacagg aatgtcttgt acccttcgct
     7021 caacatcgtt cctaatgaag ctgacggagg cgtaagacct aaaccgataa agcttaaaaa
     7081 ggcttcgaag aaaatagcag atggaatact aaacatcatt tgaataataa tgacactaga
     7141 gatattcggc aaaatatgtt tgaatgcaat tttcattctt gattcaccta atgtcaatcc
     7201 agctaaaaca tattcttgat cttttaattt cattgtttgt gcacgaacga tacgtgccat
     7261 tggtatccag tttgtgatgg ccatagcagc cacgattgaa aaaattcctg gttcaaaaac
     7321 gaccaacatc aaaatcatga caacgaggtt tggaattcct gataagattt ccaaaaaacg
     7381 ttgcatcacg ttatcgacac gtccgcctaa caaaccagaa accaacccgt aagctacacc
     7441 aatcgttaca tctagtaatg cagcaataaa tgcgatcagc aacgagattc tcattcccat
     7501 aaataagcgg cttaatacat cacgtcccaa accgtctgtt cctaaaaaga aatttacgtt
     7561 tgaagggacg tttgcttgag catatttatc aacaagttca ccaccaacca ttgcttttcc
     7621 attcaatcca tcgatattga ttcccggaat acgaggaggc aaattgatat aagccacgtt
     7681 ttgggcagtt ggatcatgag gagaaacaaa gattgtgatc acagagatca aaatgatgac
     7741 caatagtaaa accattgaaa taacggcagc tttgtttttc tttaaacggc gccatgaatc
     7801 ttggataaaa ttcaaagaag gtgcagtaat ctgttcacgt tcttcttcgg tatttttttg
     7861 taatggaaga aattcatccg cagggatatc tgcaactgtt tcataacgct ttggtatcat
     7921 ttccatttat cctcggctcc cttctgaaac acgaattctt ggatcaacta gaccgtacaa
     7981 tagatccacg atcaaaataa caaaaatcag cataaatgag taaaggatcg ttacagccat
     8041 gatcgtagga taatcatttg ttgtgattga cttaacgaat tgttccccga taccaggaat
     8101 agcaaagata ttttctacca ccagtgatcc ggtcatcaat gctacagcta acggtcctaa
     8161 taaagtcatc aatggaatca aactatttcg aagaccatgt ttaaaggcga tttcccaacg
     8221 gctcaatcct tttgcttttg ctaattccac ataatcacta tgcagtactt ccaccatttc
     8281 cgtacggata aaccgagcag agtcagcaag cggcgacatg gccaacgcaa ttgttggtaa
     8341 gattgtgtac acaaagccat cccattttgc gattggcaaa acttgtaatt tgattgcaaa
     8401 gatatattgt aataaaacag caaatacaaa gtttggaatg gaacgaccta atatcgctac
     8461 gagtgtagaa gatgtatctg cccatgtatt ttgcttcatg gcagagatcg ttcctaaaat
     8521 cgtccctagg actgttccaa agataattgc ctgtaaccct aactgcaagg atggtccgat
     8581 tcttccagct aacaaatgtg cgactggctg gtttttaaat tggaaggaaa ttccaaaatc
     8641 tccttgaagc aaattagaaa gataaatccc atattgtacg ataactggtt tatccaaacc
     8701 gacttgttta tttagcatct cgattgtttc aggactcaaa cgttcctgat tcgtatatgg
     8761 cgtgcccggc agcaattgca tcaagaaaaa ggtgattgtt gcaattaacc aaagcgtgat
     8821 gatcatgaaa aagacacgtt ttaatacata tttgatataa ctattcacta atgtttcccc
     8881 ctagcttttg acttctgaca gatgtttcgc tacattatta gataatgaac caaaaataaa
     8941 ggagcaatca aatgaaaaaa aaatctatcc cctacgcagt agcttttcta ctgattctgg
     9001 ttattttaat aaaaaacctc atcaaccatt cctttacact cattcaatta tctaatgatc
     9061 tattcttatg gtcattgccg tttctgatta tcggcggttt tttatgggtc ttttcttcgg
     9121 gtttcttcga tcattttcag cgatcggtcc atcttgcacg aacaaggaac cgaaagaaaa
     9181 aaccagaatt ttcctcgctc tcgtctgcca gctacggtat gtacagcttt tggctgatca
     9241 ttgccggaat actaattgct ctttctgcta ttttcatgct tttttctttg ttaggataag
     9301 tgaatatgag aacgaggttg cgacatttgc cgcagcctcg ttttttctca tgctcacggt
     9361 attaacgatt attcttcaac gtatgtccat ttgtaatccc atgaagcgcc tgccgcatgg
     9421 tgaacgatac ctttgatttt ttcattcact aggtgacctt ctgctttttg gtacaccgga
     9481 acgacaccca tatcatcaga gatgatcttg attgcttttt ggaaatcatc ccaacgggct
     9541 tgttcatcgc cagcgtctgt tgatgaagct gctttaactg cttcgtcata ttctgcattg
     9601 ctccagcgac cacggttgta tgaattacct gtgacaaaca aatcagtaaa gctgcttggg
     9661 tctgcatagt cagcagccca tccgcctagt actgcgtcaa aatcacccga agttgagcgg
     9721 tcaagacgta cagagaatgg aacaggtgtt ggtttgactg ttacgccatc caagttttct
     9781 tggattgagt tttgaacata ttcgattact tttttcgatg aatcatcatc agaagccatc
     9841 aaatcaaatt ctaaagaatc aacgcccaat tcttttttcg ctttttccca atattctttt
     9901 gctttatctt tgtcataaga aactaatttt ccagcttctt ttgcaaagtc ttcgttcgtt
     9961 gttggacttt ttaccatatc tgatgggatc aatcctgtag agacaactga tccgtcacct
    10021 agtacttgtt ttactaaagc ttcacgatcg atagaataag aaatagcttt tcttaggttc
    10081 acattgttga atggtgaatt cttttcacgt tgattgaatt cgatatagct tgtccgcgct
    10141 tctttaacag aagtataagc tggatcgttt gcgttttgct gtgctaactc accagacaag
    10201 atcacgtcat ctgcttggcc atctttgaat aagttcaatg cagtagaaga ttcttttact
    10261 acactgactt tgatttcaga aagttttact gtatctttgt cccagtaatg atcatttttc
    10321 ttataagtcc attctgtatc tgttcctggt ccatcaaaat cttctaatgt gaatggtcca
    10381 ttataaacag ctttatcact tgcagatgca taggcatccc cattttcttc cactacggat
    10441 tgtttttgtg ggaaaaatga tgggaaagca agcagatatt ggaaatatgg tgtttgtttc
    10501 gtcaatttga tttctaattc atagtcacca actgctttga tccctagttc tgatttatct
    10561 ttctttccag ctgtaatgtc ttctgcattt tcaactggcg catacaggta agcatattca
    10621 gaagctgtag ctgggtctac tgttcgttgc cagccataaa cgtaatccgc tgctgtaaca
    10681 ggatcaccat ttgaccattt tgcgtcttct cgtaatttga ttttgtaagt taatccgtct
    10741 tcactgactt ctgccaactc gctagctcct gccggctgtg gtttgctgtc ttcatcaaga
    10801 cggtaaattc cttcataaac gttattcaat gctgtaaaac tgattgtatc tgttgcaact
    10861 gataagtcag ctgtaggcat ttcttgaggc acgactaagt taaatacgcc tcctccatct
    10921 gctgaaccgc tttttgctga actactgctt gtggctgaac tgccgccata acatccagca
    10981 agtacaaggc cgcataccgc aaccaaacca aatgtaaatt tctttttcat tttacgtacc
    11041 cctttattgg aattttcaga ataattaata ttttctgaag ttgtttaaga gtttaatgaa
    11101 taattattaa aaaatcaaca cttttttttg tctttttcat aaaataatga ttttttcgtg
    11161 aggtttcaag gaatttttca gaaaaaatag ctttgtacaa ggttttccat ggtcttccaa
    11221 aagccattaa ataattttcg ttatattgtc tattattgcc atacaaagat aaaaaagaat
    11281 a
//
LOCUS       DACDHJ010000071        11235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDHJ010000071 DACDHJ010000000
VERSION     DACDHJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.073"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            197..1456
                     /gene="lysA"
                     /locus_tag="IV223_001422"
     CDS             197..1456
                     /gene="lysA"
                     /locus_tag="IV223_001422"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8711719.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYIQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1443..2243
                     /gene="dapF"
                     /locus_tag="IV223_001423"
     CDS             1443..2243
                     /gene="dapF"
                     /locus_tag="IV223_001423"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8711720.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2246..3127
                     /locus_tag="IV223_001424"
     CDS             2246..3127
                     /locus_tag="IV223_001424"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8711721.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3124..3840
                     /locus_tag="IV223_001425"
     CDS             3124..3840
                     /locus_tag="IV223_001425"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8711722.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3846..4541
                     /gene="dapD"
                     /locus_tag="IV223_001426"
     CDS             3846..4541
                     /gene="dapD"
                     /locus_tag="IV223_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8711723.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4557..5558
                     /locus_tag="IV223_001427"
     CDS             4557..5558
                     /locus_tag="IV223_001427"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8711724.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5555..6916
                     /locus_tag="IV223_001428"
     CDS             5555..6916
                     /locus_tag="IV223_001428"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8711725.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6876..8027
                     /locus_tag="IV223_001429"
     CDS             6876..8027
                     /locus_tag="IV223_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8711726.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8002..9171
                     /locus_tag="IV223_001430"
     CDS             8002..9171
                     /locus_tag="IV223_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8711727.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9346..10725
                     /locus_tag="IV223_001431"
     CDS             9346..10725
                     /locus_tag="IV223_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8711728.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11006..11235
                     /locus_tag="IV223_001432"
                     /pseudo
     CDS             11006..11235
                     /locus_tag="IV223_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3741 a         1954 c         2503 g         3037 t
ORIGIN      
        1 ttttatgtat aaatagcttc taatagttcg ggtattttgt ttttataacc atttttattc
       61 ttatttttaa aaaaatattt cgaaatatac tagacaattc agaaaatttt gtttaatatt
      121 attccaataa aattcaaatc tttatcaaat gaaattccaa taaaaacgaa aaaaaagtga
      181 aaagaggaat gaaaaaatga cagaattgat aattggcgga gtcacagcaa gcacattggc
      241 agaaaagtat aagacacctc tgtatgtgta tgatgaagaa aagatgagac aaacgatgct
      301 gtcattcaag cgaggctttg tatcgaaacg ttttgataca aaagtgcttt acgcctctaa
      361 agcattccaa acgattgaga tgctgcattt agttcgtgaa tatggattcg ggctagatgt
      421 tgtcagtggt ggggaaatct ataccgcttt gcaagctgat tttcctagag atactatcta
      481 cttccatggt aacaacaaga cgccagagga actgatctat gcacttgaaa atgatttgtt
      541 gcatattgtt gccgataatt tgatggaagt tgaattatta gcagaattaa gccaacgcta
      601 tttaaaaaaa atctgtatca tgcttcggtt gaatgtcgga gtagaagcac atactcatga
      661 atatatcgtc actgcgcata ttgattcaaa attcggcatg agctatgaaa gtaaagagtg
      721 ccaacaagcc cttcagttga taaaagaaag cagctatcta gagttagaag gatttcatgc
      781 tcatatcggg tcacaaattt ttgatgttac tgcttggtta gctgaaatcg acaaattgac
      841 gaattactta accgattttg accaacctct ttccttgaat cttggcggag ggtttgggat
      901 tcgttatata caaaatgatc agccgatccc agtcgaagag gcactaaaaa aactgattga
      961 atatacggaa gaagctttgg aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc
     1021 gggtagaagt attgttggag aagctggtac aacactatac acgattggtt tcatcaaaaa
     1081 gacaccacat aaaaaatatt actttgtaga tggcgggatg acagataata tccgtccagc
     1141 tctgtatcag gcagaatacg attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga
     1201 gaaagtgacg attgcaggaa aaatgtgtga atcaggcgat gtggtaatta aagaagctta
     1261 tttgccgaaa gcagaatcgg gtgatctttt agctgtttat tccacgggag cttatggata
     1321 ctcgatgagc agcaactaca atcgagcgac acgaccagca gttgtttttg tcaataatgg
     1381 ccaatcaaga ctgattgttc gcagacaagc attttctgat ctactaaggg gggaagtttc
     1441 ctatgatcct tcataaatat aatggctgcg gaaatgattt tatcttatta gattacgcag
     1501 aagacacaga ttatagtcaa ttagccgctt ctttatgcac aaaagaaaaa tatgatatag
     1561 acggattgat cgccgtaaaa atcgatccgt tggaaatgat ctattacaac aaagatggca
     1621 gccgcgcgcc gatgtgcgga aatggcattc gctgttttgc tcgatacgta aatgatcaga
     1681 acatgatgaa tgaacaggaa ttcaacgtag agacattagc aggcacaaga aaaattacgg
     1741 tgttggatcc ctcacctttt tattgcagcg tagatatggg agtgccagat gattctccgg
     1801 aaaaaatcaa agctaatcaa acttatccaa taaaaaacca ggaaatagag atcaatggtg
     1861 aaaaagtaac gatcactagt ttatttttag gaacgattca taccgttgtt tttgttgaaa
     1921 atgcgcgtga ggaagtctgg agaaaaagag gtgaactgat ctgtcatcat cctttgttca
     1981 aagaaaaaac caatgtcaac tttgtagaag tagtcaatga acaagaattg atcgtccgta
     2041 cctatgaaag aggagtaggt tggacacttg cttgcggtac aggttgctgt gcctcttatg
     2101 tggttgctaa ggaaactggg aaaatttcag cagatcaggt agttgttcac ttggaacaag
     2161 gagacttgat gatctctggt gaggatgcga tacaaatggc tggcccagca gttcatgaat
     2221 ggacaagaat attggaggaa tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc
     2281 gatgaatgaa gagggatcag tagattatgc tggtttggaa aaattgatcc aattccattt
     2341 agatgaacaa acagatggat tattagtttt aggtacaaca ggagaatctt ctacgctcac
     2401 acaatctgag gaggagcaga tccttcaact gacagtaaag aaagtggctg gccgtgtgcc
     2461 ggtgattgca ggagctggaa cgaataatac gaaagaaacg attgaaaaag cgaagcattt
     2521 tgcttctttg ggggcagacg cacttttggt catcacccct tactacaaca aaacaagtga
     2581 tgcaggacta gctgctcatt ttacagcaat tgcagaagcc tcaccgattc cgcttatttt
     2641 gtataatgtt ccttctagaa caggcatgtc gattccaatc catgtattag taaatttagc
     2701 agaacatcct aatatcattg gtttgaaaga agcctcagga gatatggctt atgtgatgga
     2761 tgccgctcgg ttgattggag aagaattctt cctttacagt ggaaatgatg atttgatcct
     2821 accagtaatg agtgtaggag gaagcggtgt aataagtgta tgggcgaata tccaacctaa
     2881 aatagtccat gaattagtaa aagatacgca ggacggaaga tggcagcaag caaaagaaaa
     2941 gcaattaaat gcacttgaac tgatccatgc cttgttcagt gagaccaatc ctattcctgt
     3001 caaagcagcc atgtcattat tagacttacc ttccggacct ctacgcctgc ctttagtttc
     3061 tctttcaaaa gagaaaaaga aacaattagc tcagcttttg ttaaaagaaa ggacggttga
     3121 aaaatgaaaa tcggactgat tggcagtgga cagatgggaa atagagtcga agaagttgca
     3181 caagaaagag gagatacggt ccttctttta cggacggcac ctaaagaagc aactacgctt
     3241 gcttttacgg aactgccaga actgcttatt gatttttctc atccagataa tttagagaaa
     3301 atcctttcgt atagcttatc atatcaactt ccgttagtca ttggcacgac aggttataca
     3361 caatcacagt tccaagaaat aaaagaacat gccaaacatg tacctgtgat gtatagtgct
     3421 aatttttcgt tcggcatcat ggtgatgaat caactaatca agcaagcagc tgcaatgttg
     3481 caaggctggc agatcgaact gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct
     3541 ggtactgcaa atatgctgct tcaaacaatc caagaagtgc aaaagctgca gcccgtatac
     3601 aactggcaag ggaaaaaaag agaagaaaac caaattggtg tacacagtat tcgtgcaggt
     3661 tctttacctg gtgaacatga tgtattgttt gctgctactg atgaaatatt tacgatcaaa
     3721 catgaatctt tttctaatcg aatctttgct gcaggcgcag tagaagcagc ggactggttg
     3781 aaagatcagt caccaggtta ttacaaatta gaagatttat taatggacaa aggggtgtaa
     3841 aaaacgtgga agctcaagaa atcatccgct acattcaaac ggcatctaaa aagacgcctg
     3901 tgaaagtcta tctgaatgta accgagccaa tcgaatttaa agaaagtaaa gtttttggtg
     3961 aaggaagcag cttcgttgtt tttggtgact atgagacgat tgctccggtt ttagaagctg
     4021 aatcagaaaa aatcattgaa atcgaaatcg aaactgctgc aagatacagc gcagttcctt
     4081 tgctagatat taaaaaaatt aatgctcgta tcgaaccagg tgctattatc agagatcaag
     4141 tgtcaatcgg caacaatgcc gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa
     4201 tcggtgaaaa cacgatgatt gatatgggtg ctgtcttagg tggacgagcg acagtgggca
     4261 aaaactgcca tattggtgca ggagctgtct tagcaggtgt gatcgaacca gcatcggcca
     4321 aaccggttat cgtagaagac ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg
     4381 tccatatcgg aaaagatgca gtcgtggcag caggagcagt ggttttagaa gatgtggcag
     4441 cagaaactgt tgtcggtgga atacctgcac gtgtattaaa aataagtgat gatcagacaa
     4501 aagaaaatac agcattgatt gctgcgttac gcgaattata gaggagatgg agaaaaatgc
     4561 gcgaaataga tacctgtatt gtgggggcaa ccggattagt cggccaaacg atgctcaaag
     4621 tgttagaaga atatgatttt ccagtaggac gattgaaatt attagcttca aaacgttcag
     4681 ccggtaaagt ttgtgtattc aagaaaaaga aacacactgt acaggaattg acagaaactt
     4741 catttgaagg atatgattta gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg
     4801 cgccaatcgc acgaagtcag ggactatttg tcatagataa ttcctcagca tggcgggaag
     4861 attcggatat tccattaatc gttccagagg tcaatctgga agactataca ttgaatcggc
     4921 tgattgcaaa tccaaattgt tcaaccattc aagcggtact tccgcttaaa gctttacaag
     4981 aagcctttgg tatccagcga gtcaattatt cgacctatca agcagtttct ggttccgggc
     5041 aaaaaggcat tgaagatctt catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt
     5101 ataatattag ccaaacggtg attccggaaa tcgatcagcc tttggaaaat gggtatacaa
     5161 aagaagaaca gaaaatgata aatgagaccc gtaagattct gcatcagcct gctttgccag
     5221 tatcagcaac ctgcgtacgt gtgccaatcg aaaatggaca tggagtctct gtggcagttc
     5281 aactgcaaaa agagtttacg acggaagaaa ttagagagtg tctcagccag tttccaggga
     5341 ttcaattaga agatgatcta gcagcacata aatatccgac gagcattctt gctagaggaa
     5401 cagatatggt ttatgttggg aggatcagaa aagacacttc tttagagaac ggattgttgt
     5461 tctatacgac tgccgataac atacgcaaag gagccgctgc gaatgccgta cagattgctg
     5521 cagctttaat gcagaaaata gcggaggtgg caagatgagt aatacgctga aagtagcgaa
     5581 attcggcgga acatcgatgt ctcatgaaga gcaattcaaa aaagtccgta caattgttct
     5641 ttcagatccc aatcgccaag tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga
     5701 tgacaaagta acagacttac tttatttgat ttatgcttac cttcagcatc atgttgattg
     5761 gacacccctt tggaaaagga tttgtgatcg tttcattcaa gtaagagatg ttcttgccct
     5821 gcagttacct attgaagatg aactagcacg catacagaaa caactggaag acgaaaagat
     5881 aactgaagat tatttagtaa gtagaggtga atatctgact gctcaactga tggctgagta
     5941 tcttggttac cagtttattg acgcaaaaga cctgattttc tttgactatg atgggaaaat
     6001 cgatcaggaa aaaacagcac aggaaatcac aaaagcattt aaaagatatc cgaaaatggt
     6061 tgttccaggg ttttacgggg cgaaccctgc aggaaaagaa aaattattgg gccgtggggg
     6121 aagcgacatt accggcgcga tactggcgaa gatgttgcaa gcggaaagtt atgagaattg
     6181 gacggatgtt tcaggaatca tgatggctga tccgcgaatc atcgatcatc ctaaaatcat
     6241 cgaggaaatc tcttttagag aactgagaga gatggcttat atgggggcta acgttcttca
     6301 tgaagcagct gttttcccag ttcaagaggc tgatatcccc atccaaataa aaaataccaa
     6361 tgatcctttc gctcaaggga caaaaatatc gaatcacgaa atagaaaagg aaaatggact
     6421 gacaggaata gctggacgca aggattttct ttccatcact ttattcaaac gtcatatgtc
     6481 cgatgaaatc gggtttatct ggaaagcaat gagcattttt gctaaacatg agatatcaat
     6541 cgaacatatt ccttcaggga tcgacaatat cggagtgatc gtctcagcag aagcgattgc
     6601 cgataagtta tttctcatca cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt
     6661 cgtgatcgaa gacttagctt tgatctctgt cgttgggggt ccgcataaag aattgattgg
     6721 attatctggg aaagtgttat cgatattgaa caaattagaa attcgcacat cgatcttatc
     6781 acaaggagca caggagttga atctgatcat cggtgtaccg aataatcaat atgaaacagt
     6841 agtgaagggt atctatgaag ggatggtgaa tacggatgct gaaagaatat cgaatggcac
     6901 tccatcaaat tcctgaaatc gggtttcatg aattcaagac aaaaaaatat ttatatgaac
     6961 aagtaaaaaa cagcgaggcc gttatccatg aaatcggcga aacaggacta ctcctttatt
     7021 ttgataatca gcaagaagaa acgattgctt ttcgaacaga tatcgatgca ctgccagtca
     7081 aagaagaaac gaatctctct tttgcttcta agcatgaagg ttatatgcat gcatgtggtc
     7141 atgacggaca tatggcgatg cttttagggt tgactgactt tataactaaa cacattgagg
     7201 atcttattta taatgtagta ctgatctatc agccatcaga agaaatttcc ggtggcgcac
     7261 aaagcgttat tcagtcagga ttgttggata aatatgaagt acaagcaata tttggctttc
     7321 atctatggcc tggactagct aagggagaag ttttctctcg accaggacct ttgatggcac
     7381 aaagcagtga aacagacatt atcattaaag gccagtctgc acatattgct tctagtgaaa
     7441 aaggaatcga tagtctggag gcagctgtac gatttttgaa gaatgtttat gattttgacc
     7501 ggcaattacc ggcagagtgg aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga
     7561 tccgaaatgt tttagctgga gaagttattg tatccggaag tatccgttct tacagttcag
     7621 atatgcaaaa ttatttgaag aggggattag accatttagc agaacagttt cactctacag
     7681 ctactatttc ttttcgctat aatgacggct atccagctgt aatcaaccat agaggcttat
     7741 atgcagcact gttagattca aacaaactac atgaattaga tgctccagtt cttcaagccg
     7801 aagattttgg catatatacg caacaatatc catgtgtatt tttcttttta ggtgtcggtg
     7861 atacccctgc tttacatgat ccgacgttcg attttgatat ggatgtttta gaaaatggtg
     7921 tagaatggta caaaacgatt ctttttacag aaaatcttct tgtaaaaatc gatagcagtt
     7981 caaaagagga gcgagtaaat aatgatgaat ttatccaatc gatttagtcc taggctttct
     8041 agaatagaag tatcaaaaat acgacaattc gatcagcaaa tatcgtcgat accagatgtg
     8101 atcaaactca ccctgggaga accggatttt ccaactcctg aacatgtcaa gcaagcagga
     8161 atagcagcga ttgaagaaga cttttctcac tataccggaa tgagaggatt agaggaactg
     8221 cgggaagcgg cttgtatatt tcagcaacag cgttatggat taacttatga tccgcaaacg
     8281 gaagttttga cgacagttgg tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg
     8341 gaagaaggag acaaggttct tattccagcg cctgcttatt caggatatca gccattagtg
     8401 gaactagctg gtgctgaatt gatcccgata gatacttctg acacagggtt tgtctgtcag
     8461 ccggaacaat ttgaacgagc atttgaacaa tatggatcag ctgtcaaagc agtcatttta
     8521 aactatccta acaatcctac tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct
     8581 gaggttttaa aaaaatatcc ggtatttgtt ataagtgatg aagtatatgc tgaattgact
     8641 tatagtggaa cgcatatgtc catagctact tatcttccag aacaaacaat cgtgataagc
     8701 gggttatcca agtcacatgc gatgactggt tggcgagtgg gctttatttt tgctcaaaaa
     8761 gcccttattg acgaactaat taaagtccat caatatctgg tgactgccgc tacaacgatg
     8821 tcgcaaaaag ctgcggctga agcgctaata aatggggtga aagatagcga gaatatgaaa
     8881 gagcaatatc aaacacgaag agactatctg gtccagcaat tggcgccttt aggatttgaa
     8941 gtgacccagc caaacggtgc attttacttg ttttgcaaac tcccagctgc cattcaaact
     9001 gactcttggc agttttgtgt agaattagcg gaacaggcaa aagttgcgtg tatcccagga
     9061 attgcatttg gcccagaagg agaaggttat atccggattt cttatgctag cagcatggaa
     9121 aaattacatg aggcctgcaa acggatcaaa gactttttaa cagaacagtg attcatagat
     9181 tttatcaaaa aaacaatcgt taattttgcg gtatattctg caaggagacg attgtttttt
     9241 aaatttagtt caaatgaacg cgtttctttt gattcataac taatgtaatg ttaagtttaa
     9301 attgtttaga atgattttaa aagaatattc aaggaggttt catcaatgga aaaaatcaaa
     9361 gttgtcattg taggtgcatc tcatggaggg catcaatcga tcttggaact attgacacga
     9421 tacggtgaaa atgtagatat cacgctattt gaagcaggag actatatttc cttcatgtca
     9481 tgtggtatgg agctgtattt agaagacaag gtaacagatg taaatgatgt acgtaatttc
     9541 agaccagaaa actttccgca atccaatgtt catattttaa ataatcacga agtaaaaacg
     9601 atcgatgccg ataaaaaaac agtaacggtt actagcgcat cagataacga aacaaaagaa
     9661 tatccttatg acaaattgat tttgagttct ggtgtcaaac caaacaatct gccagtacca
     9721 ggagcagatc tagaaaatgt ctacttgatg cgtggataca attgggcaac caaaatcaaa
     9781 gaaaaattaa atgatccggc tattaaaaaa atcgctgtcg taggtgcggg ttacattggg
     9841 atcgaagcag cagaagcaag ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc
     9901 gatagacctt taggcactta tttagatgct gagatgacag atattttaga acaacaccta
     9961 aatgaaaacg gtattgaagt tttgacaaat gcaaaaatcg aagcttttac tggtaacgga
    10021 aaagttgagg caatcaaaac aagtgaaaaa gaaatcccag cagatctagt cattcaagct
    10081 gccggtgtga aggcaaatac agaatggtta aaagggatcg tcgacttaga tgaacgtggc
    10141 tggatccaaa caaatgaata cttgcaaacg aatcttccag atgtgtatgc agtaggagat
    10201 gctacacttg cttattctat cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt
    10261 gcacgtagag aagcacgtta tgtagtaaaa catctttttg aagaagtacc aaacaaaccg
    10321 ttcggcggag tcgtaggttc ctctgcatta agtgtctttg actatcattt tgcagcaagc
    10381 ggattgaata gctttacagc agcaaaagca ggagtagatg tacgagtgtc ttattatgaa
    10441 gatacgttac gaccaaaata tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa
    10501 ttattctcgg atccaatgac ccatcaattg ctaggaggag cagtattatc tacctatgat
    10561 gttacaggtc aaggaaatgt attggcacta gcgatccaaa acaaactgac tttagaagaa
    10621 ctagcagaag ctgacttctt cttccagcca ggctttgacc gacaatggag catgttgaat
    10681 ctagccgctc aacaagcgtt aggagaagaa ccatttgtag aataagaaaa cgaaatattt
    10741 gaaaagaggt tgtgaaaaag ctgccttgca tcaagtaata agaacagcca agtaaaaaca
    10801 gcttcttatg tttttaactt ggctggactt attaccgcag atgcaagctt ttgaacaccg
    10861 tttattttca aaaaagggac tgtgacacga tttgtgtacc gacccccaaa agttagacca
    10921 aaaaatctaa cttttggagg tcggtatttt ttatggttaa atatactttt gaacttaaat
    10981 taaagatcgt tcatgattat ttggatggca aaggcgattg gaccgtaaaa atccagatca
    11041 agagattgaa gcggaaatgc ttaaaattcg ggagaagcac aaggattatg gctgtttacg
    11101 gatgaccaat gaactaagaa accgcggttt ctctatcaat aaaaagaaag ttcagcgctt
    11161 gatcaagaag ctaggaataa aagtaacggc atacacgcgt aaatcacgtc gctacaattc
    11221 ttatcgagga aaagt
//
LOCUS       DACDHJ010000072        11227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDHJ010000072 DACDHJ010000000
VERSION     DACDHJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.074"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..196
                     /locus_tag="IV223_001433"
     CDS             <1..196
                     /locus_tag="IV223_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8711729.1"
                     /translation="ETGEKFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNF
                     IRFFKKKESITPGEYRKKYL"
     gene            complement(654..1526)
                     /locus_tag="IV223_001434"
     CDS             complement(654..1526)
                     /locus_tag="IV223_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8711730.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            1826..2800
                     /locus_tag="IV223_001435"
     CDS             1826..2800
                     /locus_tag="IV223_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8711731.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            2822..3514
                     /locus_tag="IV223_001436"
     CDS             2822..3514
                     /locus_tag="IV223_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8711732.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            3480..4988
                     /locus_tag="IV223_001437"
     CDS             3480..4988
                     /locus_tag="IV223_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8711733.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            5062..6165
                     /locus_tag="IV223_001438"
     CDS             5062..6165
                     /locus_tag="IV223_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8711734.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSICWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            6168..7505
                     /locus_tag="IV223_001439"
     CDS             6168..7505
                     /locus_tag="IV223_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8711735.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            7492..8370
                     /locus_tag="IV223_001440"
     CDS             7492..8370
                     /locus_tag="IV223_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8711736.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            8367..9290
                     /locus_tag="IV223_001441"
     CDS             8367..9290
                     /locus_tag="IV223_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8711737.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            9287..9697
                     /locus_tag="IV223_001442"
     CDS             9287..9697
                     /locus_tag="IV223_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8711738.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            9701..>11227
                     /locus_tag="IV223_001443"
     CDS             9701..>11227
                     /locus_tag="IV223_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8711739.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     A"
BASE COUNT         3693 a         1887 c         2403 g         3244 t
ORIGIN      
        1 ggaaacagga gaaaaatttg gtgattttgt tcaaaatcat cgtttagagg tagcaaaaaa
       61 atggttgaaa gagactaacc tctccgtaaa agagattgca gaaagattgc aatatcgaaa
      121 tccgcaaaat tttattcgtt tctttaaaaa gaaggaatcg attacgccgg gagaatatcg
      181 aaaaaaatat ttgtgaatca agaggctgtt tttcttgtgg actgattcaa gcaataaaag
      241 aagttagaat aaccctagta tttgtattta tggatagtta aatttcaagg ctaagcacgc
      301 aaaaagaact gtgacaaaag tcttgtcaca gttctttttt acgaataaat ggcgttcaag
      361 agacaggcta tcgacaataa ggcataattt tgtgaaatat gggaagctat ttttcaaaat
      421 cgcttcttat tgctcctgtg ggttactcgt cgcaatgttt gtgagagttt ttatcatcgc
      481 tcaaggctga acgtctcttt cacaacctct gatacacggt gttccaggag tagattcttc
      541 gaaaaataag ccgaaatttc acaaaaattt aagaagcaat tttcgtaaat gccttcttat
      601 ttcttgaagc tgaccacttc tgacacaact accattttgc gttcagtaca taatcaccct
      661 ttgacagaac ctacgagtgc tcctttgaca aaatatttct gaacaaaagg ataaacaatc
      721 agcataggca ctgttgcaat aacgataaca gccattttaa ctgtttgtgc tggtggaatc
      781 acatcaacgg cactactttc tgcttgcatc ccacttgata caatgacgat ttggcgtaaa
      841 agaatctgta tcggccattt agatgaatcg ttgatataca aaatagcagt catataagtg
      901 ttccagtaag taacagcata aaaaagcgag atagtagcaa tagacggtaa cgctaatggc
      961 agcataattt tgaaaaatac accaaaatct gtgcaaccat ccatttttgc tgattcttcc
     1021 aaactatccg gcaaggcttg gaaaaagttt ctcataatga tcatattcat tgcattaaca
     1081 gccacaggca ggatcatggc ccagtaagaa ttgaccagcc ctagagaacg aacaactaag
     1141 aacgttggaa tcatcccccc gctgaacaac atagaaaata caacaagaaa gttgacgaaa
     1201 cctcgaccaa acaaatactt tctggaaaga ccgtaggcca tcagtgaagt tgcacacata
     1261 ctgacgatcg ttccgacacc tgtaacacca atcgaaacag caagtgctct aaaaatggtc
     1321 ggcgtagaaa gaatgtagcg atacgcgtct aaagaaaagg tagtaggaaa aaggataaac
     1381 tttttcgcga cgacttcttg tgtagaagca aaagatgtag cgatgatatt taaaaaagga
     1441 acgatacaga tcaaagctag caaaattaga aacgtattgt tgaaaagtgt aaaacttctt
     1501 tctcctatag agactttctt tttcatatag ataggctcct cctcaaaata aattaaatgt
     1561 ttctgtttct atatgttcaa tttatcatcg gtgaaagatt acgtatataa tcatttccta
     1621 tgctaagtgt cagatagaaa aaaatagaag gtttgctatg cgtagtttat ttgtgaaaaa
     1681 atacacttta tcgttttgta tagcacaaaa atcattgcta tgtaagcgtt tttacttacg
     1741 gtaaaaatga ttatatacgt aattcagata gttatctatc atgaagtcat aagcagcaaa
     1801 cattcacaaa actaaggagg ggcttatgga aagtgtaaag aaacaagaac catttcaact
     1861 gacgctatcg aaaaaagaag aaaagctcag aagacgaaaa aaaataattg caggaatcaa
     1921 aacgaataaa tttttatatg tgatgattct gccgggagtc ctttatttct tgatttttag
     1981 gtatcttcct atggggggac tcgttattgc ttttcaagat tatcaaccat ttttaggaat
     2041 aatgggaagt ccttgggtcg gtttcaaaca tttcattcgt ttgtttacag aaccgacatt
     2101 ctttacatta ttaagaaata cgttgatctt gttcggatta aatatcttcg tgttttttcc
     2161 gatgccaatc attttgtctt tactattaaa tgaagtaacg aacaaacgat tgaaaaacgg
     2221 gattcaaacg atcatttaca tccctcactt catgtcttgg gtaatcattg tttcgattac
     2281 ttatgtattt ttgaatgttg acggaggggt attgaatgaa ttccttgcaa caatgggatt
     2341 tgaaaaaatc agttttctaa catcccctga atggacacgc acagtatata tcggacaagt
     2401 gatctggaaa gagttgggct ggtctacgat catctacctt tcagcgataa ctgcagtcga
     2461 tacacagttg tatgaagctg cagaaatgga tggagcagga cgcttacgca aaacatggca
     2521 tgttacattg ccggcaattc gtccagtaat cattaccttg ctgattctaa aaatcggtca
     2581 gacattagac ttagggttcg agcatatgta cttgttattg aattcattga atcgaagcgt
     2641 tgcagaaatt ttcgacacct atatttatac agctggattg aagaatggac agttaagttt
     2701 cagtacagca atgggattgt ttaaaggggt cgtaggattg tttatggtaa ttttagctaa
     2761 taagattgcc aaaaagctag gagaagacgg cgtttactaa gatagaaaga gagggaaaga
     2821 tatgctgcag tcattagata aaataaacgg aaggctaaat cgtattttac agttggttta
     2881 tttgaatttt ctgtggggcg gtttagtcat cgcaggtttg ggagttttta ctttcggtcc
     2941 tgcatcgtat gcgatggttg cagtgattag aaaatggctg cgctcaaaag aaacgtttcc
     3001 tttgacgaaa acgtacttca catattttaa agaaaactat cgtgaaacct tgctgatgag
     3061 ctggatatat ggcgccatcg ggattgtttt acttgtggat ctttctttca tacacaattg
     3121 gtatcttagg atcgctttgt atattgctgc gttcatatat gtgctctcag cactctatat
     3181 tttcccaatc atggctcatt atgattggaa aggatatttt ttcaaaatga aaatggcatt
     3241 tttattcgga ttctcacatc ttcattactc attagtgttg atattaagta tggtagggat
     3301 ttatctgatg atcatccaaa caatgccagg tgttttaacc tttatcggtg gcagtctctt
     3361 cttttccgca gtcacgtgga cagcaaattc tatattttgc catttggaat ggaagaactc
     3421 atatacagaa aaaacagaag aaaacaaaaa tattattaaa acagtgaagg agaaaattta
     3481 tgaaaaaagc aacgctcgta aaattaggtc ttagttttgc aggaattcta gcacttgccg
     3541 catgcggcag cagttcaagt aacagttctt cagagacaga agatggaaaa gctaaaatca
     3601 cttggttaaa tattttgcat catgcttcac cgcctacaga cacgattgtg aacttgatcg
     3661 aagaaaaaac cgatacggaa ctgacattca actggattcc tgatgcttca aaagaagaaa
     3721 gattaacaac tgcattggct tctgatgaac taggagatat cgttacactt caaattgcga
     3781 tgttgaaaaa ctcttctgtc cgcaatgcgt taaaatcagg cgtattctgg gatgtatcag
     3841 aatatttaaa agactataaa aatttaagtc aaatttctga agatagaatc gaagctgcga
     3901 gtatcgacgg tgccttgtac ggcgtaccga tacaaaaaga ctacgctcgt gctggcttag
     3961 tcattcgaaa agactggttg gataatctag gactagatgt tccaactacc atggatgagc
     4021 tttatgaagt agctaaacaa tttacagaaa acgatcctga tggaaatggt gtggacgaca
     4081 cagttggttt tggcgatcgt gcgcctagtg atattcgtta taccagcttt aaacttttca
     4141 cttcttattt tggagcacca aatggctggg ctgtagacga taatggcaaa ttcactccag
     4201 aatttgatac agaagagtat atgaaagctt tgaatttttc aagagatcta tttgagaatg
     4261 gctatttggc acaagacttt gcagttaccc aaaagactga tcagcaagaa caatttgcac
     4321 aaggaagaac tggaatctac actggaatgg tggatatcaa gaatctgaag acgatgggtc
     4381 aaggtattca agatgatatg gaattagtgc ctgtaaacaa gatctcagat ggtgaaggcg
     4441 gcggttatca tgtttggtca gaaggaaatg gtattggcgg cttgctagca tttcccaaat
     4501 ctcaagtaaa agacgaagca caattgaaga aattacttca atttgttgat gatcttttag
     4561 aagaagatgt gtataaattg atgactggcg gtatcgaagg aacgcattat gaattgaatg
     4621 atgatggttc tatcgaattt atcgatatgg atctttggca acaagaggtg caaccgttat
     4681 caagctctcg tcctagtgaa gtgacgttct ctttcaaaga tacagaccct gaaaaagaat
     4741 tctctaatca attgatcaaa gaaaatgaag aatttgctgt aatggatcct tctgtgccat
     4801 tggattcacc gacgaacaat gaagtaggat cagaaattga aaaaatcgtc attgatgcta
     4861 cggtgcaata catcatggga cagcttgacg aagatggatt caaaaaagct gtagaagact
     4921 ggaaagcaca aggaggcgat cgaatcacac aagagtacga ggaagcatac aaagcagcgg
     4981 cacagtaata tagacgattg ggacaaatac ttcttggtat ttgtcccaaa tcctatttta
     5041 atttttaaag gagagcaaat aatggaaaac tggtcaaaga aattagcaaa aagtatcatg
     5101 gaaagaacac ctcgcttata tgaagaaaag tggtataaag gaaaatggtc ttatgactat
     5161 ggcgtcgtac ttaaagggtt ccaattactt tgggaacgaa cccaagaaaa aatttatttt
     5221 gatttcataa aagacaatat cgactatttc gtgcaagaag atggaacgat ccgtggctac
     5281 agcgttgaag aatacaatat cgaccatgta aatacaggaa agcttttctt tttattgtac
     5341 aaagaaacag gcgaagaaaa atataaaaaa gcagcagaac tcctttccca gcagttggca
     5401 aaccatccac gcacatccga aggagctttt tggcataaag aaatttatcc ttatcaaatt
     5461 tggttggatg gcttatatat gggttctcct ttttatgctg aatttatcca gaattttagt
     5521 gaagaaaaag attaccaaga tgttttgaga cagtttgaaa tcagttataa acatctattc
     5581 gatccaaaaa caaatttgct gattcatgca tgggatgaaa agaaggtcca accatgggct
     5641 gatccaaaaa caggtctatc ccatcatttt tggagccgat cgatttgctg gtatttgatg
     5701 gctgcagcag atacctatga attacttcat gaagaaacag attgttcttt gctgaaagaa
     5761 attctagaaa agacactggc tgcattactt ccatatcaag aagaaaacag cgggacatgg
     5821 tatcaagtaa caacagaaac aaaacggaaa ggaaattatc tggaagcttc cggcagtagt
     5881 atgtttgttt acgcaatggc taaaggaata cgtttgggac tgctgaacaa agaagaatgg
     5941 cttccacaag tgaagaaatc acatcaagga ttacttgatg aatttgttct ggaaacaaaa
     6001 gaaggctggc tgaacttgaa taaaaactgt caagtagcag ggttaggcgg agcagatcag
     6061 cgagatggtt catatgctta ctacatcagt gaaccaatta tttgtaatga tcaaaaaggc
     6121 gttggtgcat tcttgcaagc attgattgaa gtggaggcat tataaatatg tatgatattt
     6181 tgacatttgg cgcatcaatt gatgaattga atactgaagc aatacaacaa gccattgatg
     6241 ctgcagcaag tgacggaggc gggaccgtag ttgttccagc aggtgaattt ctaacaggag
     6301 ctttattttt aaaaagcaat gtcgaacttc atttaagcgc aggagcggtt ctgaaattct
     6361 ctgacgatcc aaaagattat cctgttgttc attcacgttg ggaaggagtc catcgaaaag
     6421 tttatgcttc ctgtatctat gctcaaaatg tagagaacat ctctgtcact ggtttcggta
     6481 cattagatgg aaatggaaaa aaatggtggc atacttttag aaatgaacca gataatctcg
     6541 cttatcctcg tcctaaattg atgagtttcc ataactgtca tcgaattact gtgaaagata
     6601 ttaaattgat ccagtcacca agttggacga tcaacccgat tctttgcagt aatgcaacgt
     6661 ttgataactt gacgatcttg aacccagcgg attcaccaaa tacagacgga attgatccag
     6721 aatcatgcaa aaatgtgcgt attagtaatt gccatattga cgtgggagat gactgtattg
     6781 caatcaaggc agggactgaa gatacgtacg aaaggatcgc ttgcgaaaat atcacgatca
     6841 caaattgcac gatggtccat ggacatggcg gtgtcgtatt aggaagtgaa atgagtggta
     6901 gtattcgtaa tattaccatt tcaaattgta tcttccaaga aactgatcga ggaatacgat
     6961 tgaaatctag acgaggtcga ggcgggatcg ttgaagatat tcgagtatcc aatatagtca
     7021 tggataatgt gatgtgtcca tttatactga atctttatta tttctgtggt ccgcgaggga
     7081 aagaaccgta tgtatgggaa aaaaaggctt atccgataga tgaacggaca ccagcattca
     7141 gaaggatcca tttttcgaat atcacggcaa ggaatgtcca tgcttctgca ggttttattt
     7201 atggattagc ggaacaattc atacaagaaa tcacatttga caatatcgat gtctcgatgg
     7261 cccaagatgc agttcccggt gttcctgcta tgatgacagg aatagaggaa atgagcagac
     7321 aaggatttta tattggcttt gcgggtaagg tttcctttaa ccagatgtcg attgaaaatc
     7381 acgagggacc agcatttcta ttagaaaata ctaaagaaat cagtattgag aaattccaat
     7441 caaatcaatc agaaaatatg gatgaactgg ttgttcgtaa aaactttgct tatgcagttg
     7501 aatgattttt taggtgctcc atctagcaga cataccccta gtggaaaacg attataccga
     7561 gaaaaacaaa acggttttat gcttgaatac tatcaattgt acttaaatga attggaaaca
     7621 gtaccagccc tctatgctta tccagaaaaa gaaggaccgt ttccgccggt cctttacctg
     7681 cattctcacg gcggagattt ttcagtcggg aaaagtgaat tgatccatgg tgctccttac
     7741 ttagcttctc catcttttgc agaagtactg acaggaatgg gatatgctgt ttggtcgatt
     7801 gatgcttggg gatttgaaga acgcggaggt atttctgaaa gcgagttgtt caaacaattt
     7861 ttattaacag gaaaaacgtt atggggaatg cgtatttatg atgtgatcag cttgatcgat
     7921 tatttggaga caagggaaga cacagatact caacggctcg caacgattgg tctttctatg
     7981 ggaggcttat taagctggtg ggtgacagca ttggattctc gtgtcaaagt ttgtattgat
     8041 ctagcggcac aggtggacat cgaaatactt ttgctacatc gccgcctaga tcatcatggg
     8101 ttttattact atgtgccaaa tctattgaca gcttatacaa cgttagcaat ccaagaaaaa
     8161 atcgctccgc gccctcgtct tagcttagtt ggaaaaaacg atacgatgtg cttggacgaa
     8221 ggcgtactga agctgagaaa agggctggac aaaaagtatc aatcaatggg tagtccagaa
     8281 aacttttctt ctttttcttt aacaggcggt catatggaaa cacaagaaat gagaaatatt
     8341 tggaaacaat tcataaggga gcatttatga ggatgaaaga caaactgata attggaaaga
     8401 atgctataga taccccgacg gattttgaaa ctatccaaga aggaatcgat tatttggcag
     8461 gcctgccgga tagccatcca aaggtcttga tagtaaaaga aggaagatat gatgaatata
     8521 tagaaagcag attatccaat tttaaacttg tgggagcagg aacagtagtt atctccggta
     8581 atcgttttgc aaaacaggag tatcttgacg gtagccagtt agggactttc cgaagtgcta
     8641 cttttttctt agaaggatcg aatgtcgtat tagaaaatct tcacatcgaa aatacagcag
     8701 gtccaggaga aaaggtagga caggctgttg ctttattcaa ttcaggagat cgcgtatctt
     8761 taaaaaactg tcgactatct ggttatcagg ataccttatg cacgggtccg ttgcctgatt
     8821 tgcaaaaaga tggtactcct ttttgtacac cggcttctgc caatcctgag tattgtcgtc
     8881 agacctatga atattgctgg attgaaggaa cggtggattt catatttggt ggggcagatg
     8941 ccgtttttca tcagtgtcat atccatagct tagccagtaa aaatcctgga tacatcacag
     9001 cagcatctac gccgaaaaat caaaaaacag ggtatcgttt tgaaagctgt accttgactg
     9061 cagaacctga tactagaaac gtctatttag gaagaccgtg gcgtccgtat gcaaatgtct
     9121 tatttgaagc gtgtaagata ggtgggcatg ttcacccaac tggttgggat aattgggatg
     9181 acaagaaaaa tgaacagact gtcaaattct ctgaacgtaa taacaattat gaaacatcga
     9241 tcaaaagacc tgaatggata catttagaaa agcagggggt tcagaaatga aaagaaaatg
     9301 ggaactattg ctaggaatgg tcggtggcag tctatctttg attttctttg gcgggttagc
     9361 agttacttta agtaatatgt ctgcgtcaga gtttaaaaaa agttatcagt cgcttgctgt
     9421 agatcattca actttgtctt tagaaaatac atttggatta ttacaggaca tgactggtct
     9481 gtttgccgtt gtgttgttta tttcacttgc ttttttagca gtagcattgt ttttgactgc
     9541 taaaggaaaa tacttaacaa ctgccactgg tttgtatttt ataacaggat tcatattgtt
     9601 gattggcact cagtttattg cgtttccttt tgcatttttt tatttcgcag ctggggcatt
     9661 cagtttatat cgagtaagaa tgagaaaagg agcctgaatc atgcagacta tcaaagtgaa
     9721 aaatcctatt ttaagaggat tcaatccaga tcctaatata ttaagagtga aagagcggta
     9781 ttacattatt gtctcctctt ttgaatggct tccaggaatc cgagtatatg agtcgaaaaa
     9841 tttagtcaac tggtcacata caacggatat tttgaccact caagtatctt tgcaaggaaa
     9901 tcctacaaat ggaagtatat gggcgccaca gatcagttat gctgatggat tgttttatct
     9961 tgtttacaca gatgtcaaaa gtgcaggccg tccgtttaaa gatgtccaca attatttgat
    10021 cactgcatct gagatcacag gtccttggtc cgaaccaatt tatttaaata gtaacggctt
    10081 tgatccttct ttgttccatg atcttgaaac gggaaaaaaa tggcttgtca atgaactatg
    10141 ggattatcgt ctggacacac cgaataaatc cagcggtatc gtattacaag aatatgatgc
    10201 agagaagaag aagcttgatg gtcccatcta taaaatattt gcaggtacag aattagcgaa
    10261 aacggaggca ccacatttgt attttcgtaa cgggtattat tatttgatga cggcagaagg
    10321 cggtacagga agaggacatt cagttacagt ttgccgagca aagaatatta ctggtcctta
    10381 cgagcttgat cctggttttc ctatgctgac agctagtgac aaacctgctt ctacattgca
    10441 atgtacagga catgggagct tagtacaaac cccgtcagga agatggtaca tgacatatct
    10501 ttgtaccaga ccattaaacg gtgcggctat tttgggaaga gaaaccgcta ttcaagaagt
    10561 gtattggacg tccgacgatt ggttaagact taaagatagc cagacaactc cgatggaaga
    10621 aataatgatt gagacaaatg aagaggtaca acaagaaaaa gatcatcagt ttatggatac
    10681 atttgaagga gagctaaaga agaactggaa tgccagacgc attttgccaa atgctgattg
    10741 gtgtgataca aaatgtagac caggtttttt acgtctgatt tccggtgaat ctcttcaatc
    10801 taccttcgaa catcatttgt tagctattcg gcaaacggat tttacttttg atgcggagac
    10861 agcatttgaa tacatgccta ataattttaa tcagatggct ggactggtac tgtacttaaa
    10921 tgaatccaat tatatttatt gttatgttac ttggaatgag gacaagggaa aatgcctacg
    10981 tatgatagaa tcagaaaacg gagtagcaca aatagaagat tggacgattc cactcacaga
    11041 aaagaagact tttttgaaag tcactgtaaa gaaagagaca gcacagtttc atttgaaaga
    11101 cgtatcaact gaagaatgga agaaagtagg tccgcaaaaa aacatgtgta tactctcagg
    11161 agggtttact ggaaatttta tcgggatagc agtgcatgat ctaaataaaa aaagaggaag
    11221 ttatgct
//
LOCUS       DACDHJ010000073        10940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDHJ010000073 DACDHJ010000000
VERSION     DACDHJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.075"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            204..782
                     /locus_tag="IV223_001444"
     CDS             204..782
                     /locus_tag="IV223_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8711740.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            782..1711
                     /locus_tag="IV223_001445"
     CDS             782..1711
                     /locus_tag="IV223_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8711741.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1708..3444
                     /locus_tag="IV223_001446"
     CDS             1708..3444
                     /locus_tag="IV223_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8711742.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKSLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3507..4343)
                     /gene="pheA"
                     /locus_tag="IV223_001447"
     CDS             complement(3507..4343)
                     /gene="pheA"
                     /locus_tag="IV223_001447"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8711743.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4340..4849)
                     /locus_tag="IV223_001448"
     CDS             complement(4340..4849)
                     /locus_tag="IV223_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8711744.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4842..6137)
                     /gene="aroA"
                     /locus_tag="IV223_001449"
     CDS             complement(4842..6137)
                     /gene="aroA"
                     /locus_tag="IV223_001449"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8711745.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6150..7241)
                     /locus_tag="IV223_001450"
     CDS             complement(6150..7241)
                     /locus_tag="IV223_001450"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8711746.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7258..8424)
                     /gene="aroC"
                     /locus_tag="IV223_001451"
     CDS             complement(7258..8424)
                     /gene="aroC"
                     /locus_tag="IV223_001451"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8711747.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8426..9490)
                     /gene="aroB"
                     /locus_tag="IV223_001452"
     CDS             complement(8426..9490)
                     /gene="aroB"
                     /locus_tag="IV223_001452"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8711748.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9523..10545)
                     /gene="aroF"
                     /locus_tag="IV223_001453"
     CDS             complement(9523..10545)
                     /gene="aroF"
                     /locus_tag="IV223_001453"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8711749.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         3050 a         2409 c         2006 g         3475 t
ORIGIN      
        1 cggtttcttt attattcaga tacttcaaaa atttctccta aaataataaa aaaatacaaa
       61 tcaactatga caaaaggaat aatcttagca tctagaccaa agatcgaaat ggtgaggttt
      121 ctttaaaata aaaatatcct tgcattttct cctagaaata agtattttta aattacaaga
      181 ctattcttaa ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga
      241 cgattttaga tggaatgaaa cagttgatca attggtctga gcacgggttt caattaatag
      301 ggactttttc cagtccgctg aaagcgcttg atttttcttt ggtacatgca atcgatatcg
      361 tggtcacaga tgtaatgatg cctgaattaa atggaatcga attatcgcgg ctattaaaac
      421 agcaacaacc tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta
      481 aagattcttt taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt
      541 tttttctaaa ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc
      601 caatcaccaa tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa
      661 ctcgtccatt gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga
      721 aaaacggcca ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt
      781 gatggatagc gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg
      841 ctatgtaatc aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca
      901 agaagttttt ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa
      961 tgaaatattc caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa
     1021 gaaccttgct tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag
     1081 tagttattta acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa
     1141 tttcttcacg acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat
     1201 caatcgtttc tatgcattac tcaacgcttt ggctgacgag catgttggga atgaagaact
     1261 ttctttgctc aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt
     1321 ttctattctt gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa
     1381 agacaatttc gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga
     1441 aatcaattta caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat
     1501 ctttactgaa aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa
     1561 agccaagctc tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg
     1621 ctataccgaa ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc
     1681 tcaataccgt aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt
     1741 gccaaacttc tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg
     1801 tttatttatc atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg
     1861 gtactaaaaa agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta
     1921 gccgcttttg attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat
     1981 atggaacagc ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc
     2041 catccaatga tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga
     2101 aacgacatgc tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga
     2161 gtgaatgaac gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc
     2221 gataagaaag cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt
     2281 tacggatatg ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta
     2341 gtaaatcctg agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca
     2401 aatgaagaaa atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa
     2461 acaacgattt caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca
     2521 ttactcagtc aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt
     2581 attttgtata atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat
     2641 cgacacacaa aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc
     2701 ttaaaagcta ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc
     2761 aatcaattgc taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag
     2821 aaacgttcgc ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat
     2881 acgctgacta gcttgaaatc tttgatttgg cagaaaaaaa cagatgaagc agttcaagga
     2941 atcgacagtt tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca
     3001 gtcaaggaag aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc
     3061 gaaagaatct cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat
     3121 atgatccttc aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa
     3181 ggattcatca cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat
     3241 aacggcgatg gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc
     3301 ggcatcagcc atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg
     3361 atccaatcag tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa
     3421 aatcaatcag aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta
     3481 aataaatcaa tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga
     3541 taacagccca atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct
     3601 actaatgtca ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta
     3661 gttttcaatg gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc
     3721 agcattttat gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt
     3781 actggagctg actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg
     3841 ttcaactcat tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt
     3901 tctaatgaag caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca
     3961 gcagttgttg attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt
     4021 tgtgattgtg ctagtgcttg tggatgcgaa aggatttttg taatctttgt tgctggattg
     4081 cctaaaagct gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt
     4141 ttggaaagaa gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg
     4201 acagccagat cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac
     4261 gcagtcaatt gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct
     4321 ggccctagat agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct
     4381 tttctggtgt ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa
     4441 gtcgttgtgc caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa
     4501 tagacgtttc atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa
     4561 gaacaatcgg ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac
     4621 cagtcgcgat aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc
     4681 gaaagctttt ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct
     4741 caatctttgt atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga
     4801 ttgttgtttt tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct
     4861 cctgacacaa gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca
     4921 gctccttcta gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg
     4981 attcgatgat cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg
     5041 atcagaccgt ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc
     5101 gcatcaattc tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt
     5161 ccttctgctt gagtagccac taaagctaga atgggcaatt catcaatcag acgcggaatg
     5221 atttctccac caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat
     5281 tggttctgtt cattgtgatt cgtctgggta atcgctgcgc ccatctcctc caacacatct
     5341 aagataccag ttcttgttgg attgaccccg acatttttta acaacagttg gctttccgga
     5401 acaagaagtc ctgctgctag aaagaaagca gctgatgaaa tatcacctgg aacagttatc
     5461 tgttgaccag ttaatttttg cggtccggtc actatgatcg ttttatcttc cactgtgatc
     5521 cttccgccaa attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg
     5581 attacggatg tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca
     5641 ctggctacag gcatattgta tcggataggt gataaagagc gggttcctgt cacagtgatt
     5701 ggtagatact ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc
     5761 atcactcgcc ccattggacg tttagacaac gaagcatccc cgaacagagt agtcgtaaaa
     5821 ggctgtccag ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtca
     5881 attgattcgg ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcacctct
     5941 tcctcttctt cgatctcgac acctaattgt tggaaggcat gtaaagtact cagacaatct
     6001 tctgcgcgca aaaaattttg tatagtcgtc gttccttcag caattgctcc aaacatgatg
     6061 ctgcgatggg aaattgattt atctgctggt atcctaacag tccctcttaa tccatgtatt
     6121 tgttgtaata attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta
     6181 gacataatga ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt
     6241 attcctaaaa tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct
     6301 tttccgatca gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat
     6361 aaatcaaaaa aggcaggaat cgcgccttgc ttatggacag gcaactggtc tcgactttct
     6421 tttgcccgat tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct
     6481 atttgtgtca tttcttgttg ccaatctgcc aatcgttcca ataatatctg cttattgctc
     6541 aacaaaatat ctgtccacat gacaggatct gaggaagcga tcctagtgat atctcgaaag
     6601 cctcctgctg ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg
     6661 actaagccag ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt
     6721 tctcttgctg taagagttac atattttgct ttggttccgc aaaatagttc ttgcagtttt
     6781 tcaacatctt ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt
     6841 ttagcatctg ctgcctgtat acctgattta tgagaaccag ccattggatg tccaccgaca
     6901 aaccgaagat ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc
     6961 gtaatgatga cgtgatcaga caagttgatt ctttcaagga catcaagata agctaaggtt
     7021 gtacggacgg gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca
     7081 cttgcaacgt aatcaacgat ctgctgttct actgcttgct cacgggtttt tgactgacta
     7141 tcccaaccaa ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca
     7201 ccaatcagtc caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta
     7261 gtaatgtttc aagtagtcac ggtactcctt cactgcagct ttcaactcaa caagcgaatc
     7321 acttgggaat tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac
     7381 gacgctagct gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc
     7441 ttttgtatcg atatcaacac tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac
     7501 cccgcgaatg acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt
     7561 ggtacgtcgg aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc
     7621 tggtaatgaa ccagaagtga atccgacccc aaattctacg cctttaaagg cattgatact
     7681 aaccacagct tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa
     7741 tccagctgga acgcctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt
     7801 ctttgtctga tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac
     7861 ttctgacgct tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc
     7921 ttggattcca ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat
     7981 tttcttggca attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc
     8041 aagcacattc cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc
     8101 tggacgcgga cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga
     8161 catgacagtt gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc
     8221 aattgttttg ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat
     8281 ccttcctcct ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc
     8341 tgacagaggt aatcctgctg gtagtccttc aataatggct gttaattctg gtccatggga
     8401 ttctcccgca gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg
     8461 tgggaacagg aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc
     8521 ttcctcttgc tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat
     8581 cgtacgtaat cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag
     8641 ttggagacaa gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc
     8701 cttctccatg actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat
     8761 gaccaaaatt taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc
     8821 gtttgatttc caacgtagca gtaatgattg gaacagcatg ttccagaaga tctttttcgt
     8881 ctttcagctc gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga
     8941 tttcagcgat tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa
     9001 tcaatacacc agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga
     9061 ctgctgtctt cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa
     9121 cgaaatggat cccacgcatg aacgtcgagg ccacgaatgc tgctagatca cccaccattc
     9181 ctccaccaag tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt
     9241 ataactcagc tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga
     9301 ctggttcaaa attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa
     9361 cgtttgtatc agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc
     9421 cagcctgttc tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta
     9481 cagtaagcat tcgtttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat
     9541 cttttccatt gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc
     9601 attcaacgat tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat
     9661 catgccatct gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat
     9721 tcctgtacca tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg
     9781 gacagcactt aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc
     9841 gataaaaatg acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc
     9901 attgatcgta ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc
     9961 agatagcagt ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc
    10021 agccaccata tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc
    10081 atcagccgct tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatatgg
    10141 agaagtacgt ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat
    10201 tcgcgcacat tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac
    10261 gaaactgccg tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa
    10321 ttctcgagaa gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata
    10381 gcgtgtaaat gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg
    10441 ttctgttcct tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt
    10501 ttttatttgt tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt
    10561 cttttgttta cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt
    10621 aattcctact aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat
    10681 gacccagata atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc
    10741 ttcaacgagc cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc
    10801 tacgtggctg taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt
    10861 ttgtacccac gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca
    10921 gttttgtaag ttttctagat
//
LOCUS       DACDHJ010000074        10797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDHJ010000074 DACDHJ010000000
VERSION     DACDHJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.076"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1355
                     /locus_tag="IV223_001454"
     CDS             <1..1355
                     /locus_tag="IV223_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8711750.1"
                     /translation="FQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALTLGI
                     AFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFFAVT
                     IGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILELKLG
                     DMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKIGIF
                     SENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLADAMS
                     EFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAGIIN
                     YSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKKGQL
                     ITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAELQK
                     "
     gene            1377..1637
                     /locus_tag="IV223_001455"
     CDS             1377..1637
                     /locus_tag="IV223_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711751.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1651..2403
                     /locus_tag="IV223_001456"
     CDS             1651..2403
                     /locus_tag="IV223_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8711752.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2497..3102)
                     /locus_tag="IV223_001457"
     CDS             complement(2497..3102)
                     /locus_tag="IV223_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8711753.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3155..4129)
                     /locus_tag="IV223_001458"
     CDS             complement(3155..4129)
                     /locus_tag="IV223_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8711754.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4507..5286)
                     /locus_tag="IV223_001459"
     CDS             complement(4507..5286)
                     /locus_tag="IV223_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8711755.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5353..6624)
                     /gene="celB"
                     /locus_tag="IV223_001460"
     CDS             complement(5353..6624)
                     /gene="celB"
                     /locus_tag="IV223_001460"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8711756.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6777..8174
                     /locus_tag="IV223_001461"
     CDS             6777..8174
                     /locus_tag="IV223_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8711757.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8255..8695
                     /locus_tag="IV223_001462"
     CDS             8255..8695
                     /locus_tag="IV223_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8711758.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            8843..9169
                     /locus_tag="IV223_001463"
     CDS             8843..9169
                     /locus_tag="IV223_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8711759.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9170..9919
                     /locus_tag="IV223_001464"
     CDS             9170..9919
                     /locus_tag="IV223_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711760.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(9999..10454)
                     /locus_tag="IV223_001465"
     CDS             complement(9999..10454)
                     /locus_tag="IV223_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8711761.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3415 a         1912 c         2205 g         3265 t
ORIGIN      
        1 ttttccaaac cattatcaat tatgttttga acttaggatc agccattttt gttcccctga
       61 ttattttgat acttggattg ttggcaggga tgaaattaaa aaaagcattc atgtctgctt
      121 tgactttagg gattgctttt agcggaatgt ctatggtcat tggctatatg tctaacgcag
      181 taagtccagc atcagaagca ttggctaaaa atacgggaat cagtttgcct gcacttgatc
      241 ttgggtggac aggtgcagca agcatcacat ggtcgtggag ttatgctttt atattttttg
      301 cagtaacgat tggggtcaat tttgtactgt tactttttaa ttggacgaag acgttaaatg
      361 ttgatatgtg gaatgtttgg gggaaagctt taactgctta tttagtttat tatgtttccg
      421 gtagtttggc tgcaggcttt cttactgcaa tggtccaagt tatattggaa ttaaagcttg
      481 gtgatatgtt tcaaaaacat atacaagatc tcacagggat tccattagta acagtgactc
      541 attttatgac atctgccgct gtcttgttgc tgcctttcaa tatgatcatg gataaaattc
      601 ctgctttaaa taaacgagcg gatacgaatg cattgaagaa gaaaattggg atcttctctg
      661 aaaactctgt aatgggattc attattggat tggggcttgg ctttgctgct gcgtatggcg
      721 tttcaggttc tcttaatcta gcgatccaaa cagctacagc attaagtttg tttcctatga
      781 tctctaaaat gttcatgcaa tctttgtctc ctctagcaga cgctatgtca gaatttatga
      841 aaaagcggtt taaggatcgt gaagtatata tcggattgga ctggccgata ttagctggtc
      901 gttcagaaat ttgggttact gctattttac ttgttcctgt gtttattggt tatgccatta
      961 ttttgccagg aaaccaagta ttgccattag cagggattat caactattcg attgcggtcg
     1021 gcggattact tttaacagga ggaaacttgg ctcgtatgct gatcttggga atcatcacta
     1081 tgcctattta tttgtacggg gccacttacc ttgcgccaat actcagtgat ttagcagaaa
     1141 aaagcggagc agtcgaggga atcaagaaag gacagttgat cacatggtca tccatagaag
     1201 ggccggagtt cagggtttta tttgcggagg catttaatag gaatattctc gctattgtag
     1261 gatgcgtgat ttttctagga ctgtttgttc tactttataa atatatgata aaagcgaaag
     1321 taccgagtga acgttatgct gaactacaaa aatagcattg aaatggtggg aaagtaatga
     1381 aaagagaaga gacagataaa ataaaatgga cagtcgctct ttgtggtaca cttctgctat
     1441 ttctatatgg actctttact cagaatatca tcatcaatct attggtcatt tttttcgcac
     1501 tagtgatcta taaatatgga aatcacgttt tattcagaga gtacgatgaa aaaagaaaac
     1561 ggaaaataga agaatcaatg aaaataaaag aagctaccaa agaaatattg agagaaaaaa
     1621 gttttataaa aagataatgg aggggtcatt atgtcagatg gacgtatgtt atttgagaga
     1681 gaacgacagg acatggcacg tatcgtaaga gtgattttcg aaaggaaaaa cacaaatgtc
     1741 gcaggaggga atttttcctt taaaaccact gatgatcaag ggaaagacta catcatcatg
     1801 acgccgacca tgatgtcaga agcctatcta ggagagattt ctcctgcaca ggttctagta
     1861 gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc atttaactcg tgaaatcaat
     1921 atgcatgaag cgatgtatga gactaatcca gctatcaaag ctgtcctaca tgctcatgcg
     1981 cctaatgcaa tgttctgggc gactactggg ttgcctatgc ctaatttgac agaagccaca
     2041 gctaaagtaa aagagattcc tgtcatggac tttgagccta attgctctga agaacttgct
     2101 gaattgacaa gcgactatgt aaaaaaacag catctcgaat taccgcatat gttgctgctc
     2161 aactctcatg gcgtattgat caacacaggt ggagaatcag gaatcgaagc tatccataaa
     2221 gcactgcaaa tccttgatac agttgaatgg aatgcagaaa ttgcctacaa gcagacgatt
     2281 tttcaagcat tagggattac tgacggtttt tattcaaaag gtgagaaaat cgggggattg
     2341 gaagagttga aacataaaac ggctatttat aataaatctg ttattgcagc tggtggagac
     2401 taacctatct gacacaaaaa actacgactt tcacaaaatc tgatccagat cgatatctgc
     2461 ttcaggttgt attatgtaag tcgtagttct tttcttttat ttagttaaga ggtggtctgt
     2521 ccattccctc gcttgcttga tttcgtccat cgtcaactga tgacctgtta cagttagctg
     2581 aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc aattctttaa aggaagcagg
     2641 ggaaacgatt ggatcgttat caccataaga aatccagacg gttttatcct ctaatgtgga
     2701 acggctttga tgaacaccta gagacatcgg atggaaaaac aaccctttat ttagctcggt
     2761 ctcacgctcc aataataaat gagcagcgat gtttgcgcca tttgaatagc cgacaagcag
     2821 ccagtcactt aacggtatca gttctttttc gctgatttct ttgatttttt catggagttt
     2881 atctgtttct ttttccaaac tgtccaggtc gaattgattc aatccattac gcttgaaaaa
     2941 acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa agcaatgtac tgtttggtgc
     3001 aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca cctgtgccat gaagtaagat
     3061 caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc attttgttcc ctcttttctg
     3121 tttctataga tgcaaatgaa gtagcatgaa gagattattc acgtttttta tttgcatcac
     3181 ttgtatcgaa aggacggaca taagcttcaa tggcttcacg acgttcttct aagaatggag
     3241 gaagagatag tttttctcct gctgattctg ctggttcatc ttcaaggaat ccaggaccat
     3301 cagttgctaa ttcaaacagt acgtgatgag cggctaggaa gtactcagat tggaaataga
     3361 aacgatcgac gaaaccagaa tttggcaatt caatcttatt gattacttga atccagtatt
     3421 ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg gatatttcca aatccttcga
     3481 ttgcttttgg caagtctgtc cgatgttcaa taatgatgct tgcaccattt cccccttcac
     3541 cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc tttgaatcct aagatttttt
     3601 caagaatcaa gcggatatgc tctaattgac tgacacggac aaagacaggt ccaagtcctg
     3661 tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg aatgccagct gctacacctt
     3721 gattgttttc gtcagagatc aattgataat actgattatc gaaatcttca aattcgatat
     3781 attttttgcc gaaacgttca ttgattgctg aatgagtaac gtcatatttt tcaaaacgtt
     3841 ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa agaagcacga gagatcgtat
     3901 ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa gaaagtcata tctgttcctg
     3961 gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc gatgtcatct tggttaactg
     4021 tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa gcggtagatt tttgtagcgc
     4081 tagatgtcat tgctgtgacg tggtgaattc ctctgatttg tgtatccatg ataaatttcc
     4141 tcctgttaac ttacaaaaag taagtagttg atttagacat agtatagagg gtgcatctct
     4201 attttgcaat taatttgtct tctaaagtat caggaaggaa tcaagaatgg aaaagaggag
     4261 ggacggatca ataaaataaa aagacttacg actagctgta tcgatttcaa aaaaactagc
     4321 tttaaaaagc taactttaga atcattacgg gtggtcataa gtctttttat gctgagttat
     4381 tttttcattt ttgagctaat gcttaggata gaatagaatg ttgatttcac gatctcttct
     4441 tgttacttga aactgcttac cactgtcacg atctcttggt aaacggcttt ttcacaacct
     4501 ctttggttaa gttgaacgca catccagcac agaatagttc cgaacatcaa atcggtttcg
     4561 gctggaagaa tattcaatcg gctgtccatc tttgagatag acgacttgct gaacttccag
     4621 tacaggatcg ttttctgcac agttcaaata ttgctgatca tattcatcac ttttatctgc
     4681 agaaatagca cgataagccc ctgcaaaatg gacattcaat tcttgatgaa gatgatcata
     4741 gatagaggct aacaaatgtt ctttttttaa acccggcaca agatggactg gcataaaggt
     4801 atgctctaaa acatatggct cgccatctaa gatccttaac cggtgaatat tgtagacagg
     4861 ttgctcggca tcgatcatca atcgttcttg gatatattga ttagggaatt caacatcaaa
     4921 atgaatgatt tgactcttca aagaacgatg tctgcgtttc atttggagac tcaaaccttc
     4981 atactcgtta gcgggcgatg tgtctttttg cataaatgga tgattcaaga ttttcgttcc
     5041 tgatcctcgc tgcgaataga caagtccttc cattgctaag atattgatgg cttttttgat
     5101 tgtcatccgg ctcgcaccaa attcttttac caagtcgact tgattaggca aaaaactatc
     5161 gagaggatac tcttttgaac ggatacgttc tcttaaaata tttgcgattt cttcgtattt
     5221 tggcacgtta ttcacctctt ctagagtata acgaaagatt atgcttccga caactcgaag
     5281 tagcatctgg aacattgttt attcgaaaaa agaggtcatg acaagttttt gtcacaaccc
     5341 cttaatgatc tattaagcaa tatctaaatc tggttgttta tctagtactt tcaggaatgg
     5401 gtaccagata aaggcaacga tgacaaagtc gatgacttgc agcataccac caagccatga
     5461 atttgttgct aagacaccgc ttgcaatgat tggcacagtc catggaacag agacgccagt
     5521 tggtgcaggg acaagtccag cagccattac caaataattg aacgtcgtta cgattagagg
     5581 tgccacaacc caaggaataa ggattgttgc attcaatacg atcggtagac cgaagatcgc
     5641 tggttcattt acattgaaaa taccaggtgc taaagctaat cgtccaacat cacgatactg
     5701 tttcttttta agaacaaagg ccatcaataa aacgacagct aaagtcattc cagagccacc
     5761 aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg atgtgtggta attcttttcc
     5821 atgttgataa gcttctaagt tatcaagcat cagtgtattc caaatcgggt ccattacaga
     5881 gttgacgatg atttgtccgt gaagtccgaa gaaccataag aattggacaa agaacaaagc
     5941 aatcaatgtt gcaggtaaac tacttcccaa accagttaat ggtttttgga tcacttcata
     6001 aacgacgtca tgtaagttag cattgaaaat accagtcatc acagcattga ttactaagaa
     6061 aatagttagt gtcactacag cagggatcaa agcggcaaat gattttgtta ctgcaggcgg
     6121 aactccatca ggcattttga tctgccagcc gcgtttcgtg attctgcagt agatttctcc
     6181 agccaagaaa gcagcgatca ttccaatgaa cattcctttt gcgccaagac gatcaagtga
     6241 aagcacacca gtcacatctt caccgccagc ggttttcatt gcaaaaggcg taagaatcaa
     6301 gaagctggca aaagacacgg caccaccgaa aataccttca acgtcataag attttgataa
     6361 ataatagccg ataccaaaag taacaaaaac cgacatgatg ctcattgttg cattttgccc
     6421 attaccgaat aaattcgata aagttccctt tgttgcgtca ctgaaaaaag gcaagttatt
     6481 gataacgaca acgatcgaac cgaacatcgt taaagggaag gaaagcatga aagcatcccg
     6541 cagcactgta aggtaacgat tttgccctaa taaattagct aagggcatga ttttctcaga
     6601 aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa tttatttgca atcgattaca
     6661 tacaaagtat aacgccaaaa attttaaatg tctagtcatt ttcataaaaa gtattgattt
     6721 attttttagg gttcgttatg attacggtat caagaaaaaa gagaggaagt aaaaaaatga
     6781 aaacctacac attcccagaa aatttttggt ggggttctgc tgctagcgga ccgcaaacag
     6841 aaggaagagt aagtgatgat ggtaaaggag aaaatatttg ggatcattgg tatgaattag
     6901 agcctgaaaa attcttcgat caagtaggac ctgaaaaaac ttcgcaagtt tacacaaaat
     6961 acaaagaaga tgttcagtta atgaaagaaa ctggccataa cacatttcgt acatctatcc
     7021 aatggagtca tttgattcca gaagggacag gtgaaatcaa tcaaaaagct gtttcttttt
     7081 atcgagatta ttttactgaa ttgctggata atggtattga accttttgtg aacctttacc
     7141 atttcgatat gccactacca ctgcaagaaa aaggcggatg gttatcaaga gaaacggtcg
     7201 acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt tggagatctg gtcaaaaaat
     7261 ggtttactca taatgaacca atcgtacctg tagaaggagg ctacttgtat caattccact
     7321 atccaaatca tatcaatatg aaagaagcgg tccaagtagg ctatcatgag aatctagcaa
     7381 gcgcaaaggt aatcaaagcc taccatgaag gcggacatga tggtgagatc ggaatcatct
     7441 tgaatctgac accaagctat ccgagagatg aaacgaaccc agaagatgta aaagcggcga
     7501 agatcgctga cgcattcttc aaccgatcat tccttgaccc tgcagtgaaa ggagagtttc
     7561 cacaagagtt gatcgaaatt gtcaaagaat tggctataat gccagagatc catgaaggag
     7621 acctagaact catccgtgaa aacacgattg acctgctagg aatcaattac tatcagcctc
     7681 gtcgaatcaa agcaaaagaa actccaatca atcacgaaca tagccccatg ccagatgatt
     7741 attttgataa ttacgacatg cccaataaaa aaatgaatcc atatcgtgga tgggaaattt
     7801 acgaaaaagg aatctatgat attttaacga atacaagaga aaactatggt aatatcaaat
     7861 gctatatttc tgaaaatggg atgggggtcg aaggagaaga acgatttgtt aacagcgaag
     7921 gaattattga agatgattac cggatcgagt ttgttacgga tcatttgaaa tacgtccatc
     7981 aagcaatcca agaaggaacc aattgtgtgg gttaccacat gtggacatgt atggacaact
     8041 ggtcttggac aaatgcttat aagaatcgct atggctttat cgctgttgat ttggcacaag
     8101 acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa aacggtctct gaaaaaaacg
     8161 gatttgatgc ctgatatttt ttgataaaac gttcacaaaa tgtttgttct aacactgtaa
     8221 tcgtgctaca ctaacagtga aggaggcgtt tacaatggta gatacttatc gtaatatttt
     8281 agtagcaatt gatggttctg aaaaggcaga aaaagcattt cttgaggcaa tcacgattgc
     8341 caaacgaaat caggcaacgt tgcacatcct gtatgtcaat gaagtaacag gcaattactt
     8401 tggtgatttt gcatttgtta caacaagcct tcaggaagaa ttggatgagg ttgcggaaaa
     8461 ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc ctgacagata tcgagacata
     8521 tgttctttac ggttatccaa agacactgat tgctaatttc catgaatcga aagaaccaat
     8581 cgatctgatt gtcatggggg cgacaggtct gaatgcggta gaacgtgcac tagtaggttc
     8641 cactacttcc tatgtagtca accacgcatc atgtaacgtc atggttgtaa aataaataaa
     8701 ttaagggaac agaaagcaaa aaagcataac gcttcttttg ctttctgttc cttttatttt
     8761 tttgtaaaaa attactttac ctgatgaagt aatcatgcta ttctaattac aacgacagat
     8821 ggagtaatat ggaggaaaga aaatgatttc aagtgatgga atacgcggat acaatgatgc
     8881 gattatttta tcgatcctta acaaaaacga ttcttatgga tatgaaattt ctaaaacgat
     8941 tcgtgagagg acaaatgatt cctatgtgat caaagagaca acactttact ccgtttttgc
     9001 ccgtttagaa aagaatcaac tgattgtttc gtatcctgga gaagtgactc atggaaaaaa
     9061 gcggacatat tataaaatca ctgaaatagg gcgtaatctt ttagaagaaa aaaagcaaga
     9121 gtggctgacg acgaaagacg tagtcgatca atttttggag ggaaattaaa tggatacaat
     9181 ccgtaattat ttagacagtc tttttatcgg agtacctcaa agtaccgaga tagataagct
     9241 gaaaactgat ttgttggcaa atatggaaga ccattatcat gaattgatgg gagaaggaaa
     9301 aaatgaacag gaggccatcg gtacagtaat ttcgacattt ggttcaatcg atgaattatt
     9361 agaagaacta gatgtcgaaa aaaaacatca agctgacgag acggaaacaa acacagcttc
     9421 tatttattta agcgaagcag aaaactactg gaaagaatac agagcggcaa gtctccaagt
     9481 ggcatcaggt gttctattca ttagcttatc ttttgcaagt tttcttttct tttgttcagc
     9541 aggatatgtg ttcatgggta tttcttgtct gattttcggt atagcattag ctgtcggttt
     9601 ttttattgct tctggtatga aaatcacacg tttaaatcac tttttgcacc atcggaaaat
     9661 acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa gagtatcagc gaagttttgg
     9721 cttctcactg attgcaggaa ttggattatg tattttttca cttttcccac ttttggcttc
     9781 tttaatgtgg tatatggatg gtagtatcgg tgcctctata ttttttgtta cggtaggaac
     9841 gggtgtcttc ttgatcattt atggcagtct agtccgtcat tcttatcgtc aattcactca
     9901 aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa tattctattt atccggcgta
     9961 gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt atatataata atttaaaaat
    10021 tcatcatcta aatccgtaga attttctaga ttgcttaccc atgtaaaatc gttatagaca
    10081 gatttcagtt gacttctgcg ccattgtaca gggataaaaa gacgcatgct atcttcattg
    10141 aatccgacta ataatttctt tccgtcagta atgatgggac gttgtaacaa agtaaaatgc
    10201 gagacgatca gatcgacaag ctctgaggtc gtacattggt cgatttggtc tttcaaattt
    10261 ttataatcaa tcgagcgttt agctaataga tcgtcaaatc cgttttctgt taatgacaat
    10321 aaataaaata tctctttcct tgttaaaggc tgtttaggtg tgaaaattcg ttctctgtaa
    10381 ggcatttgat ggtctttcag ccagtttgcc atcagatggt aactatgtgt gcgagaagaa
    10441 tagatcgtaa gcataaaaaa actccttaaa tagtccatta atttgttaga tctgttgaga
    10501 taaataatag gtaaggagct gtgcataagc attgatcaaa acaacccaaa catcattaga
    10561 aataactttc taaagtctta aacttgcgac gagaaacaac tagactaata gaaaaaatgt
    10621 cttagccatt tggcttcctg tttgtttttt tgcttatgct gcagctcctc gggaaagggt
    10681 tctaacaatt tgaagaaggt tgatgattga tctagtttaa attgtgtgaa atttgaaatc
    10741 tggtgttttt actagatgaa tcacctaaca actatgatta taacttatag aacttct
//
LOCUS       DACDHJ010000075        10475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDHJ010000075 DACDHJ010000000
VERSION     DACDHJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.077"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..515
                     /locus_tag="IV223_001466"
     CDS             <1..515
                     /locus_tag="IV223_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8711762.1"
                     /translation="DCNGQLDGETEMQRIVKKCGSTEVKSKSKPINMTVTITAHVPME
                     VYRRFNGLKQDERIKSGIYSYGPDSVGEDFSLAAEIVDDFEEKNKLIGMLACTSNTGL
                     TFSIENGADEVAALELETKVMQDEFGKFYHEAIVAELEEDLTDQWMTNLSADVIKKTS
                     VVTTTATQSQ"
     gene            535..897
                     /locus_tag="IV223_001467"
     CDS             535..897
                     /locus_tag="IV223_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711763.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            900..1082
                     /locus_tag="IV223_001468"
     CDS             900..1082
                     /locus_tag="IV223_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711764.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1099..4530
                     /locus_tag="IV223_001469"
     CDS             1099..4530
                     /locus_tag="IV223_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8711765.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            4581..5318
                     /locus_tag="IV223_001470"
     CDS             4581..5318
                     /locus_tag="IV223_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8711766.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            5328..7619
                     /locus_tag="IV223_001471"
     CDS             5328..7619
                     /locus_tag="IV223_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711767.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7643..9769
                     /locus_tag="IV223_001472"
     CDS             7643..9769
                     /locus_tag="IV223_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711768.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            9932..10378
                     /locus_tag="IV223_001473"
     CDS             9932..10378
                     /locus_tag="IV223_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711769.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            10380..>10475
                     /locus_tag="IV223_001474"
     CDS             10380..>10475
                     /locus_tag="IV223_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8711770.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         3658 a         1622 c         2226 g         2969 t
ORIGIN      
        1 ctgattgtaa tggacaatta gatggcgaaa cagaaatgca gagaattgta aaaaaatgcg
       61 gttcaacaga agtaaaatcg aaatctaaac caatcaatat gacggtaaca attactgcac
      121 atgtaccgat ggaagtttat cgacgcttca atgggttgaa acaagatgaa cgtattaaat
      181 cgggaattta ctcttatggt cctgattccg taggggaaga tttctctctt gctgcagaga
      241 tcgtggatga tttcgaagaa aaaaataagc tgattggtat gttagcatgc acttcgaata
      301 caggattaac attctctatt gaaaatggtg cggatgaagt agctgcgtta gaactagaaa
      361 caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt gcagaacttg
      421 aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt aaaaaaactt
      481 cagttgtgac aactacggcc actcaatcac agtaaaaaaa acggaggtag cgaaatgaac
      541 gaagattact caaaaattga actaaacgat ggaacaattt tgaatttaga acctaaactg
      601 aatatcaaga aattattgat gatcaataga gattttaaca cagacgagtt tgcaaaaatg
      661 actgtgggaa aaggatccat ggatatttct gttattcaag gtgcaaaggc tgtgtatatt
      721 gcttaccgcc aagcgaacat gactgattat atttcattcg atgaatttat cgataaatgg
      781 gattttgata tggctactgc cagctatatt tatcaattga tgatgttcaa acaagcacgc
      841 gatgcttatc aaaaagaatt tgaaaaagca aataaggaaa aaaagcttca aaagtaaaaa
      901 tgccaaagct cttagttgaa acgtgggtcg atgtctattc gatgttgacc gacgtttttt
      961 ctatgccttc agatttggtt ttaagcgata tctgtttaga tgacattttg caaatggctt
     1021 acaacaagag tgcttatgaa ggatggaaaa actatgcaat aaaccaatcc cagaaaaact
     1081 aaagaaagga ggtaaaaaat ggctaaaaag agaacagaag cagaagtaac attcatagct
     1141 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
     1201 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
     1261 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
     1321 actagccaac gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag
     1381 aaacttgaga gagaactgat taaccaacaa acagcgcaac aacgtttgtc aaacgaaatt
     1441 gataaaacga gtaatgcact agctcaagca aaaggcgaaa tacagacgta cgagtctaca
     1501 atgcaacagt tggatagtga acaaaaaaat gttcaagcta gtgcttctct gattgaatcc
     1561 gaatacaaaa aatggcaagc aactgctggt caatcagctt ctgaatccga gaaattagcg
     1621 aaagcccaag aatatgtttc tcaacaatct gaaaatgcag agaaaacgat agatatcctg
     1681 agacgacagt tagaagctac acagtctgag tttggcgcta catccacaga agcaatgcag
     1741 atggaggcga agcttaatga tgctgaacgt gaatttgaag agttaggaca agctgctaaa
     1801 aatgtagata caactaactt ggacgatatc ggaagcaaaa tagatatgaa taatctaatg
     1861 gaagcttctg acgttttaag cgacattggc gataagctta cagaattagg gaaacaagca
     1921 gtggactctg ctaatagtgt aggtagttcc cagagtaaaa tacaagctaa ttttggtttg
     1981 actaaacaag aggctgaaga attaacgaat gtagccagag acatttatta taaaggtttt
     2041 ggagaatcgt tagatcagtc cacagatgca ttgattttgg taaagcgtaa tttaggcgat
     2101 ttaaataatc aagatttaca aaatatcacg gaacaagcta tggtcctaga aaacaccatg
     2161 ggcgctgata tggatgaaac gttacgtggt gtaaatggct taatggtcaa tttcggcttg
     2221 agcgctcaag atgcaatgga tttaatggtt tcgggtactc aaaacggttt agataaaacg
     2281 cacgaattag gcgacaatat ggcagaatat agccaattat ggagtcaaat gggatattca
     2341 gctgatgaaa cgttcggaat gcttcaaaat ggtttagatg cgggtgctta taaccttgat
     2401 aaagtcaatg acttagttaa ggaaatggga atatcgttaa cagatggtcg atttgagcaa
     2461 aacatggata tgtttagtga aagtactaga aaagcttttg aagagtggaa aaatggcgga
     2521 ggaacacaaa aagacgttat taattccatg attcaagatt ttagcaatat ggatggtcaa
     2581 tacgaccaat taaataaagc ttctacaatt tggtctgcac ttggcgaaga taacgcgatg
     2641 aaagttgtcc aatctttgac tgatgttaac catacatttg atgatgttag tggatctgca
     2701 caaaaaatga atgaagattc tactactccg ttacaagaat taaacggaaa aatagctgaa
     2761 ttaaaggatt cattagctcc tataggcaac acaatcatag atgcactcga accagtaatt
     2821 gattttctag gaaagatggc tgatgcgttt aataatcttc cacaaccagt acaggatttc
     2881 attgttgcta taggtggtct tacagcagca tttggcatac tgttaccggc aatactagcc
     2941 gtgtcatatt tatttggtcc gatgatgctt ataattggag gaattatagc tgttatagca
     3001 ggcgttattg tggcaattaa gaactggggt gcaattactg actggtttag tggtttatgg
     3061 aaaaaattta ctgattggtt gggtgatact tgggaaagta taaaagacgg agcctcatca
     3121 gtttgggatg gggttaaaga aacctggtct ggatttgtag attgggttca agatatttgg
     3181 caaggagttt ctgattggtt tggagagcta tggagcggat tagttgaagg agcttccaac
     3241 atctggcaag gagtccaaga aacttggcaa acattcgttg attgggtttc aaatatttgg
     3301 aacggagtca aagaagtatg gtcgattatt tgggcagaca ttgtaggaat tgttcaaata
     3361 ccatggacat taataacgtc attgattcaa gctggtatta atattatcgt gggtattttt
     3421 gatgtagctg gacagttatt gggcgcagct tggcaagctg tttggacacc tatttctgat
     3481 ttccttaaaa atacttggga tactatgaca caatggataa gtatcgcttg gaatgggatt
     3541 gtaactacat tccatactat atttgatcca gtagtggcat ggtggaatgg tatatggaca
     3601 gctattagta ctacggcttc aaatatttgg aattcaatta gtgcaacagc ttctagtatt
     3661 tggaacagta tcaagaatac aatcactagc ttggtacaag cagctgctac agtaattcaa
     3721 aatatttggt caactgtatc tagttggtta ggtggaattt ggaattcaat cagctctaca
     3781 gcatcaaata tctggaatag cgtgactagt agtataagca atgctataaa cgcagctaaa
     3841 agtgccattc aaagtgtttg gaatagtata tcttcgtgga ttagcggaat ttggaacggt
     3901 atcaaaaata ctgctttgaa tctttggaat ggaattacaa gcactattag ctctaaagta
     3961 aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc taacaggtat tgtttccgac
     4021 atattcaata atgttaaaag tacaattgct aacatatggg aaggcatcaa aaagactgtt
     4081 agcgctccaa ttgattggat tagagacaaa atcagtggca tttttgataa tttgaatatt
     4141 tctataccac atattccgtt accggaattt atcatggaag gcagctttaa cccgctaaaa
     4201 ggtcaaatcc cccatttgcg tgtaaaatgg catgctaaag gaggtatctt tactaaacca
     4261 actttactag gtggaatgaa cggtgtcggt gaagcaggac ctgaagcagt tttacctttg
     4321 aaaagatctg ttttgcaaga aattggtgat cgtatcttga gtagcacatc agtttcatct
     4381 agggcacaaa cgattcaacc tgtgaataac tacgaattca atttcacaat tgatggtaac
     4441 gcagatgagg ttactatgaa gcaaacaact caacaaatca ttgatagcat tacaaaaatt
     4501 caaaatgata atgcttcggc atggcgttaa acaggagagt atttctcctg tttttttagt
     4561 attaaaaagg atgtgaaaaa atgactgatt gtatacattc tataatcgat ggatttcctg
     4621 attatttgca taaattggct ttagctgaaa gaccaaccat accttctcca aaaagacaga
     4681 gagttgaaac ttctgtttta ggtaggttag gtggcttagt acaagattac tcgtttgaag
     4741 atatgtcgtt tacattgcac tataactatt tagaggatgt ggaagaccat caagcgttca
     4801 agcaatcgtt ttatatcatg cgtcattggt taaactatgc aaagaaatta gaattctctg
     4861 acgatcccaa cgtctattac gttatccaga ctatcgatat tggggatgca gaaaacgata
     4921 ttgttgaatg gggagagttc gatgtaaata ttactgcgaa accattcgca agagttcaag
     4981 aagatgtacc tataaccgta gataaaccac agtcatttaa cttgctgaat aatagtttag
     5041 aagaaagttt tccaaagatt atcatcactc cttcagctac ttcatgccag ttcatcttaa
     5101 atgattatgt gtttagtttc gaaggcttag tagcaggaac tgatgtagtc attgatagtg
     5161 atttgatgct ttgctacgaa gagcaatcgg acggagatat tttagatcgg tccaacaaaa
     5221 tgaagaccat gcaatatccg acattgcaag tggatatcaa tcattttaat tgcactggtt
     5281 tgagcaaaat acaaatttat cgtaacgggt taaggtaggt gaaatagatg atcgataatt
     5341 taataactat ttacgataaa aatgacgcga ataatttagc tgaacattta tatgatacgc
     5401 aaggtttagg cgctttgtca gactggttaa cagctactgt tagcaataaa ctaaacggag
     5461 ccgagatatt tcagggtact tattcaataa acggaactaa tgcagatttg attgtagacg
     5521 gacgtattat tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta cggatttatt
     5581 atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa agctgaacct attttcaatg
     5641 atataagaaa atcggtgttg aataaatatg acagcggaac agaaaagatc actgctactc
     5701 aggcatggca aaacgcaaaa gttttagcga aaccagctat cccttcgcag ttttctttct
     5761 cgtcattagt agatacgctt gctaatgtga agatagaaaa ggcgaatttt ttagaattct
     5821 ttggtggaaa agagggatct attctagatc gatttcatgg tgagtttcta aaagataata
     5881 acacattacg tcatgaaaaa agtctaggta cggatcataa aatcaaagcg atttatacta
     5941 aaaacttaac tggtcttgac ttagagattg atgctcaaag tgttttagtt ggagtttatc
     6001 cattcattag cagctcttca gaaggagaag acgagatcac tctaccagaa gaagttattt
     6061 tcacggatta cgtggatgat tatcctgctg gatatgtttc ttttgttgat tttaaagaca
     6121 aagcgactga tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa acaaacatag
     6181 ataaacaaaa accacaagtg agtggttcga ttgaattagt accattgagg catcaaagag
     6241 gctatgaaaa atttgttgat ctagaaaaag tttcgatggg tgacggagta gatgtgtatc
     6301 atccacagtt aaaagtgaat atgtcagcga gaattgtgga atatacgttt aatgttctaa
     6361 ccaattcata cgataaatta gttgtaggaa acgtcaaaac aaacttctta gaaaacacag
     6421 agaataatgt cagcaatttg attaatgatg ccattgatca attgaaaaac ggtggcgaaa
     6481 tcagtgattt actcaatgat attgtagatc atcaaactga tatgattact ggtcaaaatg
     6541 gtggttatgt tttattagat cctaaagaag cgcctagtcg tattttgatt atggacacac
     6601 cagataagaa taccgcaagg aatgttttac aaatcaacaa tgctggtatt ggtttttcta
     6661 aaactggcat taatggaaca tatgaaacgg catggacgtt agatggcgga ttcaatgcct
     6721 cgtttattac ggctggtgaa atagtaggaa ttactattag aggtactaca ttaattagtg
     6781 atggtgctga ttatagaaca agtattgcta atggcaaaat gacttggtac tcaaaaaaag
     6841 ttaacaaaga tattatggag ctagaagcac gtgattatgt aagtgctgat gccggtattg
     6901 tatcatacac catgaaaact ggtggtggtt tcatgattag aaatccacag ggtaacttgg
     6961 tttttagtac gtgggataat ggtaataaca gaccgtttct atcttttggt gcgcccaatt
     7021 tcaggtatag caatgctagt tatgtaactt ctggcgacgg tagttcttta agcattaatg
     7081 gtagtgcggg taactcatgg gaatttaagg tagctggtag gactatgaaa tttactagtg
     7141 atggtatgct aacgttacca ggttgttttt ttggttcatg ggaagatggg aaacttgcta
     7201 ggtttgaaca atcaacggta caagtatata aagattttac tgttagaggt actaaaaact
     7261 caactgtacc aacagaacat tatggacaac gactattgaa cgcttatgaa actccagaat
     7321 attatttcgc tgattatggg gaagccgtta caggtgacaa tggtaaagtt cgtgttgata
     7381 ttgaccccat gtttgctgaa acagtaaatc taagtcggta tatgacacat gtgacaccta
     7441 cagaactagt tttgtgtgct gttactcatg aagatattga ccatttcatc attgaaacta
     7501 gtaagccaaa cgtattagtt agatggaatt tagtggcaca ccgtctaggg tatgaagata
     7561 ttagattaaa agaggataca gcatatgata gcacagtgct tgaccaaaaa cgtttttaaa
     7621 acgaagacaa ggaggtatat aaatggctag cagtttatat aatttggctt tagatttcag
     7681 caaagaatta aactacacca aagctattat ggctcgtcaa ggtgataaag ggattacggt
     7741 gacggttaaa ccgtttctaa atggcttgca gatggatacg agtggcggaa catttacttt
     7801 aaaaggaaca acaccatcta accgttacgt agataatgtt gcaactagtg taactagtga
     7861 agaagtcacg ttttctcttg atggcacatt tatgagtgaa gcaggatatt ataaacactg
     7921 ctacgtagaa tatagaaaag acaatcaaat tttaacaacg caagatatca tttttttctc
     7981 actaggagtg tctgacattt cgcaaggcca agccgatgaa tatgtttcgc aattagaaga
     8041 gttgattcga aagtacaacg aaacttttga tgcttttatg gctgaaatca aaggtagagt
     8101 ggatagctta aatcaacaga ttactgattt aactggtcaa gctaaaacgc tacaagacaa
     8161 gttagatgct ctgaaagaag aaatttctaa gttaggtaac ttacaagtga tgtacagtaa
     8221 cagcatcgac ttcggggact atgattattc ggagaacccg aatttaatgc catatattac
     8281 agaaccttgg gttggacctt tgttaggtaa tggacataca gttaaagata gtgttaaaag
     8341 ggttataact catactaaaa ctagaacagc taattccggc gacatattat cgcttggctt
     8401 aggtattccg tgtacagcgg aagctaataa taggtatctt ataaccactt tacgaccatc
     8461 tacgacatac acattaagcg ttactatgag tgtcggttca gattggactg gagagaccaa
     8521 tactataggg gttaggctta gatatttaaa tgaacaaggt ggtatagaac tgcccattaa
     8581 tgcattaata ccagcaaatg tagaacgcga taaaatggtc actcatacat ttactggtat
     8641 aacaaaagat aatgtaacta gcataacaaa ctgttatgtt gaaatattct cacttaatag
     8701 tgaatataaa ggaacggtta gtgtcagcta tgacgttaag ttaaaagcac actaccctaa
     8761 cttactagat ggcccttact ggttaggtaa agttccttta ggcgagaata ttgctgaccc
     8821 tacagttgtc tttccacaca aaactagtga atatatggtt tatggtagac gcaatacaga
     8881 aaactatatc gctgaccaaa catataccat atcaatgaaa gcaactaaac taacagttca
     8941 atcgtttgcc gtgtatattg ctgctggccg cgttaaagta ggagacatga agcctactga
     9001 ggggttagct aatacatggg agttaacttt tactgtaact aagcaacata tagatagtgg
     9061 ggtaactaat tatctggaaa tataccaata cccgtcagct acaaaaggag ctgttcaaat
     9121 tgagtggctc aaactagaaa aaggcaatac tcgaaccccg aatattagtg agtataaata
     9181 tcgtggtact ggtatgcgtg attcaaacaa tccaaaagat tatgtttggg atctagcacc
     9241 agaatatgtc gaagataatt tggccacaga tattaaaatt tctgaaatta ctggtaaagc
     9301 aaacaattat accgatggga aagtatcgga gattaattcg cagttgactg cttcaattaa
     9361 tgaagtagac accacagcta aggatgctca aacaaaagcg aatgctaatg cgactgctat
     9421 agatgaatta gacaataaga tcgatgaacg cattaatgat acagctacta ccacattaac
     9481 agttacaaac gggaataccg gatcagcaaa gctttatcgt gaaggaaaaa cagtttctat
     9541 atattttgtg gctttaaacg gaaaacgcag tggtggaaat gattcaacga tactaacaat
     9601 tccagaaggc tatcggccac caattagttt tgagcaactg gttggctcga tagaccgttc
     9661 tactttgaac agtgctcagt tatctattgg tgcagatgga gccattaaat ggcgaagaaa
     9721 ctcaagttat ggatcggatt atacctttgc aattacttac acgatttaga aaagcgtgaa
     9781 tcgatatgaa ggcagcatat agaccaattg aaccttacgg attcgagcaa atcattgtga
     9841 atgatgaaga acatttaccg gaagaatgca cagaagtcga accaccgatt ccaaattgga
     9901 aaccgaaatt caattactgg gagggaaata aatgaaaaac atttggaaat atggacgtac
     9961 tggcggagag tacgcaggaa aagtattgga cgacatgctt gtatccgttc cttacacaga
    10021 tcagccaccg cttgaaggaa ttcgtgctga tggcgaaccg ttaacaatcg ctgatcaaat
    10081 gtttgatcct aaattgaatc aatggattat tttagcgaac gcactagatc acaacgattt
    10141 aaacaatctc aaagcaatgt atgagtcgtt agaaaatgag aacggcgatt taaaacagat
    10201 caatgccaaa ctcatgctaa gcgatgtagc aattaaacag gaaaatactg cattgaaaga
    10261 aaaagcggat agtttagcac aaatcaattc aaaaatgatg cttgcttcgt tacaaaatag
    10321 caaagacatt tcagaaatta aagagcaact aaatccagct tcaaagggag gtgagtagta
    10381 tgtttagttt tagcgatgtg aaaatgatgt atgattgggg ctgtttcact aacgaacaag
    10441 taatggtttt cgttccgttg tgcattactg aagaa
//
LOCUS       DACDHJ010000076        10417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDHJ010000076 DACDHJ010000000
VERSION     DACDHJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.078"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..459
                     /locus_tag="IV223_001475"
                     /pseudo
     CDS             1..459
                     /locus_tag="IV223_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(361..1077)
                     /locus_tag="IV223_001476"
     CDS             complement(361..1077)
                     /locus_tag="IV223_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8711771.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1127..1336)
                     /locus_tag="IV223_001477"
     CDS             complement(1127..1336)
                     /locus_tag="IV223_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711772.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1613..2886
                     /locus_tag="IV223_001478"
                     /pseudo
     CDS             1613..2886
                     /locus_tag="IV223_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2993..4024)
                     /locus_tag="IV223_001479"
     CDS             complement(2993..4024)
                     /locus_tag="IV223_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711773.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4031..4861)
                     /locus_tag="IV223_001480"
     CDS             complement(4031..4861)
                     /locus_tag="IV223_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711774.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4858..5664)
                     /locus_tag="IV223_001481"
     CDS             complement(4858..5664)
                     /locus_tag="IV223_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8711775.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5670..6494)
                     /locus_tag="IV223_001482"
     CDS             complement(5670..6494)
                     /locus_tag="IV223_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8711776.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6484..7584)
                     /locus_tag="IV223_001483"
     CDS             complement(6484..7584)
                     /locus_tag="IV223_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8711777.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7762..8547
                     /locus_tag="IV223_001484"
     CDS             7762..8547
                     /locus_tag="IV223_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8711778.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8580..8963
                     /locus_tag="IV223_001485"
     CDS             8580..8963
                     /locus_tag="IV223_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8711779.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9039..9170
                     /locus_tag="IV223_001486"
     CDS             9039..9170
                     /locus_tag="IV223_001486"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8711780.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9139..9375
                     /locus_tag="IV223_001487"
     CDS             9139..9375
                     /locus_tag="IV223_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8711781.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9453..>10417)
                     /locus_tag="IV223_001488"
     CDS             complement(9453..>10417)
                     /locus_tag="IV223_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8711782.1"
                     /translation="TVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQIEK
                     GFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGINTV
                     LETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIEYPN
                     CEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLEYPL
                     ADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAAGSR
                     IELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3060 a         2452 c         2032 g         2873 t
ORIGIN      
        1 atggctgatt gaaaaagaat ataaaggcgt tcaagcgcag tttattaaag ggagattgga
       61 tgactcacct tcccattgtg aacactgtgg atcgattcgt attatccgca acggttccta
      121 tacgacacgt actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg
      181 tacccgtttt ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga
      241 tgaacatcat caactaacaa aggaactgaa gcaggcgatt ctcatagaac tgtacgaaaa
      301 tcaatcacgt aatctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc
      361 ttactcaaag attccacgac attggagccg ctcaaacggc attatcatcg actgtttaaa
      421 cgaccaatta gtcaaacaga aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg
      481 tacagcgaca ccttccatat ccagagcagt cgccacatca cggggatgga tgccatccaa
      541 attaaaagca accacgccag tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc
      601 gccgatcgct tgtaatttag gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat
      661 attctccata ccaatctcat tcaagtaatc gattgctcct gcaatgttcg gagtaccagc
      721 ttcaaatttc catggcagct ctttccatga actatcgtaa agattgacaa aatctatcat
      781 ttcaccgccg aattcgactg gctccatttg ttctaacaat tgacgtttgc catataatac
      841 accgattcct gtcggcccgc acattttatg cccgctaaag gcgaaaaaat ctgcatccaa
      901 cgcttgtaca tcaactgtca tatgcggtgc tgcttgggca ccgactggac tatcctcaac
      961 tacaacagcc tgttcaaccc tcagccgttt caagcacaac aaataacctt ctggatctgg
     1021 tttgccgacc tgaagatcat cgatcgtcac aaaggaatca attttttcct taatcatcgg
     1081 atgtgcttca aaaaaatagt ctagctcccg cagttcagaa ttgagactac tccgattttc
     1141 cagtgctgct gatccgctgc tcaaaatgtt cagcatccac tggtctccca accgcttgac
     1201 ttatcccttc ataaaaggac agttctgctg ccgtcccaac accttgttga tagtctgcaa
     1261 aaggaatttt atagccgaac tgctccagta aataggcttc agtaaatagt gatgaatact
     1321 ctcgtaaaac atccattcac tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt
     1381 ggtcatcttc tttccccctt acccaaaaca agcaaaagct gtgtcctgct ctctctacag
     1441 tatcagcata aaaactgccg tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt
     1501 tctaggactg atagtaaatc attctatcag tccttttgag cataaaaaaa caaaacatac
     1561 agaagctccg ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga
     1621 tcttcatctt atcaaactat tactaggaat caaagatgga aacattcaaa tcactcatgt
     1681 cgagaagaaa aaaatacatt caatcaacag tatcgttgtt tctgccacgc tcattaaaac
     1741 gattcagccc tgtcctcagt gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg
     1801 aaagaaaaaa tcgctggtcc ttctcaataa gtgtgcgaat caacggactt atcttgcttt
     1861 ggcaaagcaa cggtacctct gtcgtggctg tcacacctat tttacggcga atacctacat
     1921 tgttgaaaaa aactgtttta tcgccaaaca agtacattat aaaatattgg aggaattgac
     1981 tgaaaaacaa gcaatgacga cgatttctaa acattgcggc gtttcttggt caaccgtttc
     2041 gcgaacactc gcttctctgc tccccatgac aaacgtgaaa ataaattggt tgcctcgctg
     2101 ttcattggta gatgaatttc gttcattgaa aaatcaagtc ggtccctatt ctttttcttg
     2161 tatggatggc gatactggga aattactgga tattcttcct tcacgtaaga agaaagatct
     2221 catttcttat tttatgcaat ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac
     2281 agatatgaac gcctcttatg ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt
     2341 tgtggatcgc tttcacatcg tcaagcacct cattcgcagt tttaaggata tttgactatg
     2401 agtcatgaaa agcttcgacc gaaacgatcc aatacaagcc aaacactatc gacaagtgaa
     2461 ggctttatct cgacttctga tcaaacgaca ggacacgctt gtttatgata aatggacaaa
     2521 atggcgtaat tttggttggg cctatttaac cgaaagtgaa gtcgtagaac gtctactttc
     2581 tacttctgat gaactacgaa tagcttatgc ttactatcaa gaaattcttc aagcttttta
     2641 tgacaaagaa gcggacatct tctttcagct tgtaaaaaca atgcccaagt cggttccaag
     2701 agaactgcat catataaaaa aagcgtttat aaactatgaa tccggcattc gtttagcctt
     2761 agagttgcct tactcaaatg gcaaaataga aaatctgcat acccacatca aagcattgaa
     2821 acgaatagct tacgggttag gatgagaacc agaattttct taatcaatca tctgatcact
     2881 tattaaacat aaaaaaacat ctaaagcaga aaatcaattc taccttagat gttcttatat
     2941 cactctatca gtcctatttg acaaagagcc agaaatgttt gccgactata cactagcttt
     3001 gttgtgcaaa atgctgagta tcaatttcca cagggattgc tagaatatct ttcagctgaa
     3061 tgtataaggc atcgctaatt ttctcttccc gatcgttcaa ccgatccact aaaatttctt
     3121 tgccctccag cgtttgaaaa acataccgtt gaatgttgcc taaaataaat gattccttcc
     3181 atagaaccgg aatcttaacc ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta
     3241 aataataaat gcagcgattg cccttagcgg aaaactcgtg ggtatactga ttcagctcat
     3301 accattcgaa acgattatag ttgccgataa attccgcaac aaatcggctc tgaggattct
     3361 gatacaattc cttcgaggtg gaaacctgag ccacccgccc agcttccatc acaaaaatcc
     3421 gatcactgat tcgcatcgcc tcttcttggt catgagtcac aaaaatcatc gtcatcccta
     3481 gcgattgctg gatagcgcgc aaatcccgct gcagctgttt tctaattcgg gcatccaaag
     3541 cacttaatgg ctcatccagc agcaggactt tcggttcagt gaccaatgag cgagccagtg
     3601 ccacccgttg tcgttgaccg ccagaaagaa actggacatt tttatctgcc tgctccgtca
     3661 tatccaccaa tgatagcatc tcaaaaacct tttcctgaat cacctcagga ctcagcttct
     3721 ttaccttcaa accaaaggct acattttcaa aaacagtcat agttggaaac aatgcatact
     3781 gttgaaaaac aaagccgata ttgcgatcct tcgtaggctt atcggcaata ttctcattgt
     3841 ccagcacaat ctcaccgctc tttggttgtt ccaatcccgt gatacagcgc agcaacgtag
     3901 actttccgca cccgctggga cctagtaaag taaccagctc acccttttca atctgaattt
     3961 catcaatagc taaaatctgt ttttcatcaa aaaacagatt gagatcatgt atttctaaaa
     4021 acattttccg tcacaactcc tttaagggct tctttttcga ctgcttctct tccctcgtct
     4081 gcttaaggga cagctgttgc ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc
     4141 ccaccataaa cagcaaatac aaaaccatca cagcgctagc caaatggccg ttttcgttca
     4201 tccgccgcag catatagatg cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca
     4261 gcatgtactc cccaaaaacc gaagaaaaaa tcatcagaga aactagggtc acaccgatac
     4321 ggatgtttgg aaacagcacc tggataataa tcgtagacat cggggcgccc aaggtttctg
     4381 ctgcatccac taattccttc agattaatta agcgcatctg gttgttaaga ctaagataga
     4441 caatcggcaa cgccgtaata aacaatgcac ctaccaacac tacaaacatt gggataccag
     4501 tttcagcata cgttcgaagc aaagccgtta ccaaaatcac tcctggtaaa gcatacggca
     4561 gcaggagcag cttttccagc caacgattca aacgagggaa atacaaatga atccaaataa
     4621 tggtgggaat catcaacaac agaatcgtca ccaagaccac acctgctaat aaaagtgagc
     4681 gacccaccgc agcaataaat tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa
     4741 aatcactagg taaaagcgat tttgaccatt tagtggagct ggcatacaaa atcgttccaa
     4801 taatcggcag caagtaatac aggacaaagc agaacaaaat cggttttatt aactttttca
     4861 cgtctgcacc accttgcctt tcttttgaat aatcaacatt gccgttacca gcaggacacc
     4921 taaagcgact gccaacgcac tgcctaaatt aggcttggcg aacacatctc ccgacactaa
     4981 agccgcaatt cgaatcgtta aaagatttac attactgctg gtcagggcat acgctgtttc
     5041 ataggtcccc atgccattag caaataagat gacaaaggtg ctggcaatcg atggtcgcaa
     5101 aaaaggcaaa gccaccttga accaagcata cgctgatgaa gcgcccagca attgtgcgga
     5161 ctccagccat tcctttttca cttccttcat gctgggatac aaaaacaaga cccctaacgg
     5221 aatttggaaa taggtatacg tgatggtcaa tccccaccaa gaatacaaat tgaagtccgc
     5281 tagaaatggc aaaatcattt tcagcacacc tacattcccc aacaaaataa taaaagagaa
     5341 tgccagcgga atcccagcaa aattagctgc caaattggaa acaaaggtga ttttctcctg
     5401 caatttagtc gatagattca ttaagcataa acataaaata aacgtcccga ttaacccaat
     5461 cacactggag ctgacagcaa tcagtacgct gttaacaaag gcctgtgtgt aatatttgtt
     5521 gctgaaaata tcactgtaat tggccaatgt ccactggtta gttaaatcac tgtgcaagct
     5581 gcacagaacc ataaaaagca gcggcagcag taaaaatgca cagaagataa aggaggcagg
     5641 aaccagaacc aagcgttttt tcatctcttt catcccctca tcaattcttg aatgtcctgc
     5701 attttctctg acggtgcaat tcctaataac cagcaaaaca atggagccgc cagcagctgt
     5761 ggtacagttt gttccttcag cttttggcgt ttatttgaaa tcaaataaaa aggaacttca
     5821 cgatgtgcag ccaagctgcc gccatgcagt ccgtgcgcat ccatcccgtg atccgcagtc
     5881 accacgactt gataaccttt ttgcagccat tcaggaaggt aaatagccaa taatctgtcc
     5941 gcctgattaa ccgctgctgc atattcatga gaatcggcgg taaatttgtg accgacatca
     6001 tcaatattca tcgaatgtac gaccaaaaag tctggctgat actgattcac taaataatgg
     6061 gcatccgcaa ataaatgcga atcagggtaa gtgtcctcac tataaaagat gccatgctga
     6121 atcagtcgct cctcctgatg ctggatgcga tcaaccaaac gattataggg ggcttgattg
     6181 tacaactcgc tgtaccagtg gtaagcggcg gctcctgttt ttccgccagc ttccttaact
     6241 aattcaaaca gcgaccactc tttggatcgc tgagcatggt aattactata gatgccattt
     6301 tcataggctg gaacgccggt catcagtact tcgtataacg gacgtgaatt gctgggcagc
     6361 tcacttttta ccttcaataa gcttgcttgc tggtgctcaa ctaaatgatt catgaaccca
     6421 agctgggcga cggctgtgtc gtagcgacag ccgtctagta taactacaat cagtttgtca
     6481 agtctacttt gcataggtca gtacctccgc ctgccaaagc tctggaagct ctacagaggt
     6541 cttgttccaa gcttcattgt cttcaactgg caccgccgct tcataagcat cctccggcag
     6601 taatttttca gctacttctt taggcagctc taccttgcga ataggtcgcg catagccctt
     6661 tgccaaattg atttgccctt catcagataa aatatattcc cgtgctagct ttgccgcatt
     6721 gggatgaggg gcatttttat tgattaaggt tgtatacccg ctaatgatgg agccgtctgt
     6781 tggcacagtt acctcaaaac gctcggagtc aatttggtca cgataattta gtccattgaa
     6841 gtcccaaaca actgccacat cgatttcacc tttttcaaga tttgctacgc tggcatcaac
     6901 ggtggacaaa cggccttttt ctgccatttt tttgtagtaa ttcagacctg gctgaatatc
     6961 cttttcgtca cctccgttgg caatcgccgc agccaataca gcaaattgtg cttggttggc
     7021 aatcgtcaca tcccccacgc taacttgata gtcaccctct tgcagctcct tccagctagt
     7081 cggtgcttgc ttcacattct ctttatcggt gataaaggaa atggttcccg tatagcttaa
     7141 caaccaatcc ccatcatcat cctttgccca atccggaatt tcctcccaat agttggtctt
     7201 ataaggtaag gttaactctt tgctttcagc caacggaccg aagctgatac cgacatcacc
     7261 aatatcggcg gttccatttt ttccttctga ttcaaactta gccaattctt ctgcactgga
     7321 catatccgta tccacgtgac ttaagccgta ttctttttca aggtcgttcc aagtgcctac
     7381 ccaatttgcc caagtatccg gcattcccaa gctggcaatc tctccttctt ccttggcttt
     7441 ttctataatc gtatctaaag agtcgtctgc cgcagccgat tgcttcgttg cttcacctga
     7501 gccgcagcct gtcagcagga tacttactgt tgctgcacaa aataaaccga tagtcatgtt
     7561 acgaattttt tcattcattt tcattcattc ttctcccctt tcgataattc cattctaaca
     7621 aagctttgta atgagagaat cagtttcttg aaaaggtttc atgaacaatg taaaggttgt
     7681 atatggtttg taaatttcga cgaattcccg ttaaccttct aaagcttttg cggtatgata
     7741 gtagaaaaaa ggaggctccc tatgcagtat tatgatcagc atctccacac gtatttttcc
     7801 tttgattcag aggaaaaatt tgaaaactat ttagcctatc agccggagtt tttcgtttct
     7861 acggatcact ttgatttaaa caatccatct accggaaaag atgacattcc ggattatcca
     7921 gcctatataa acaaactgga ctctttaaaa gaaacctatt ccaccaaatt tctgcgaggt
     7981 atcgagattg gcgttgttcc aggacaagaa gagcggatcg tggattacct cgctcagcat
     8041 ccttatgatt taaagctggt aagtattcat caaaatggcc gctttgatta tatggacgat
     8101 gtggttttaa ccaaagatcc cattcaaact accaaggaat attttgatca gatgttgcag
     8161 gtcttgacgg attttccaga aggagatatt ttgactcact ttgattatgg attacgccgc
     8221 ttcaccttca cagcggaaga attcgcggag cattttgagg ctcagctgct aacgattttc
     8281 aaaaaggttg ttgaacgaga attggcgatg gagttaaatg ccaagagctt cttgaaatac
     8341 ggcaacgaag acctttaccg ttatgccgtt cctttgtatc aaagtgtcgg cggaaagctc
     8401 tttaccttag gctcggatgc tcatgttgcc gaagattatc agctgggctt taaaaaaatg
     8461 agtcagctgt tggcagactg tcaggtagaa aagttgctag tcattcaagg aaaagaacgc
     8521 cgcctgacgc cgctgccgat tttgtgattc ctatataata gaagaaagtg gtgaaaaaaa
     8581 tgagcaacct gtgggactac aatcaagagg cacccatcca ttatttaatt gcgcgacatt
     8641 gggatgcgtt gaaaattgag gcggtttgcc gatccttgtt agcagccgtt ccaaaacaac
     8701 aactggaaaa ttttttggtg gctgacagct tgcagcggga aaaagttcag gcttattttg
     8761 cagcctttaa agatcagcct ttggaatatt tgcatgcgca gtttcatctt ttttatcaag
     8821 tagccgcccc agatgactac aacgatttac gaggacagct gcaattgacc tttcaagcgg
     8881 atgaaaccgc ctataccgtt ttattgggaa tggctcgcct tggtgatcag gcgaaggtcg
     8941 agtggcggat tttcgatatt tgatggcagg atcaaggcgt ttcagttgca aaccttcctc
     9001 tctttcaatt agaaaaaccg cagcaggaag aagtttgtgt gctggtcacc gatgctggtg
     9061 aaattcgtct ccgcttattt cctgaccaag ctcccctggc tgtagccaac tggcgctccc
     9121 tagctgagca aggcgtttat gacaacacac ctttcggctc gtgtcattaa ggaatttgtt
     9181 attcaaggtg gcgctttgga tggttctggt gatgaagctg tttccagcta tgagggcttt
     9241 ttcgctgatg aagtgcatcg cggactttat cattttaatg gcgccttggc cttaggggac
     9301 cacggccccc acaccaacgg caaccagttt tttattgtgc aaaacacaaa ggcgcaggct
     9361 gacctgctaa tgtaaagcaa gcatacttgg agcttggagg actaccggag ctggatggtc
     9421 gttatactgt ttttggacag gtataccaag ggttagaaat cgtagcagcg atcgcagctc
     9481 agccgacaaa cagtgaagat gtaccgattg agccagtgtt cattcgacaa attaacttcg
     9541 aaaaaacatc gtaaaaagac actcactgac ttcagcaaaa gctctattct tgagccggct
     9601 gcagctagtg agtgtctttc atttatccat aagataaagc ttggattcaa taaataaata
     9661 aatgttttgg ttaaagctgt gaccagcaaa tgaggaaccg tgcgagccag ttgatctgcc
     9721 aatgcaaaca tttctttagg tgaaggcgct ggcgtgtctg ccaacggata ctccagtccc
     9781 aatgcttggt aaccgtgcat accatagcag tggtacggta gtaattctaa cggtacgaac
     9841 cgatcaagat ctgccacaaa acaagcaatc gtcagcagct cggtcaggtt catattgata
     9901 cctggaacca caggaactcg aattcgcact tcgcagttag gatactcaat taaaaagcga
     9961 cgaatattct tcaaaatttg ttggttatct aagccggtcc attgtttgtg gagcttgctg
    10021 gtaaacattt ttacatccac aaacaatgta tccacatagg gagctacctt ttggatttca
    10081 ttataagcac cacaaaagct ggtttccagt acggtattga ttccaatgta tttcgatttt
    10141 tgcaaaattt ctttagcaaa atccgcctga accagtgcct cgcctccgct aatggtcact
    10201 ccgccatccg agtgaaagta aaaaacagca tccttctcaa tctcatccat tacctcttca
    10261 gcggtcatgg tctggccaaa tcctttttca atctgcttgc tttgtgattc gggaatcgaa
    10321 caccaggcac agcgtaatgg gcagcccttt aaataaacaa cggtccgtaa accagcacca
    10381 tcatgtagag aaccctgttc tattcttaat actgttc
//
LOCUS       DACDHJ010000077        10416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDHJ010000077 DACDHJ010000000
VERSION     DACDHJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.079"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..116
                     /locus_tag="IV223_001489"
     CDS             <1..116
                     /locus_tag="IV223_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8711783.1"
                     /translation="VAGLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(178..1989)
                     /locus_tag="IV223_001490"
     CDS             complement(178..1989)
                     /locus_tag="IV223_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8711784.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1986..2351)
                     /locus_tag="IV223_001491"
     CDS             complement(1986..2351)
                     /locus_tag="IV223_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8711785.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2514..2909)
                     /locus_tag="IV223_001492"
     CDS             complement(2514..2909)
                     /locus_tag="IV223_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8711786.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2927..3889)
                     /locus_tag="IV223_001493"
     CDS             complement(2927..3889)
                     /locus_tag="IV223_001493"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8711787.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3889..4101)
                     /locus_tag="IV223_001494"
     CDS             complement(3889..4101)
                     /locus_tag="IV223_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8711788.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4122..4820)
                     /locus_tag="IV223_001495"
     CDS             complement(4122..4820)
                     /locus_tag="IV223_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8711789.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4840..5382)
                     /locus_tag="IV223_001496"
     CDS             complement(4840..5382)
                     /locus_tag="IV223_001496"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8711790.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5514..6518)
                     /locus_tag="IV223_001497"
     CDS             complement(5514..6518)
                     /locus_tag="IV223_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8711791.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6515..7504)
                     /locus_tag="IV223_001498"
     CDS             complement(6515..7504)
                     /locus_tag="IV223_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8711792.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7501..8307)
                     /locus_tag="IV223_001499"
     CDS             complement(7501..8307)
                     /locus_tag="IV223_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711793.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8473..9429
                     /locus_tag="IV223_001500"
     CDS             8473..9429
                     /locus_tag="IV223_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8711794.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9506..10024)
                     /locus_tag="IV223_001501"
     CDS             complement(9506..10024)
                     /locus_tag="IV223_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8711795.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10112..10261)
                     /gene="rpmG"
                     /locus_tag="IV223_001502"
     CDS             complement(10112..10261)
                     /gene="rpmG"
                     /locus_tag="IV223_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8711796.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3236 a         2284 c         1775 g         3121 t
ORIGIN      
        1 ccgttgctgg tttggttgct tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag
       61 aagcaatgat cgaagaaaac cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat
      121 tatttaaaaa tacgcatgaa gactttctaa aaaacagata agtcatcatg cgtattttta
      181 tttgtcagtc aatataagtt gtgctatttt atctgcctgt ctcagattcc atggatctgt
      241 ataaggtcta gctactgctg cagttagcca atccacacct tcaacaggta ttccatagca
      301 ataaaagtca gactggattt cccctgaagc atcaatcacg cgattcgtac ctcgctcaac
      361 atctatagct tctgaatgat aacttcctgt attatctgaa aatgaaaaag ggcgaacaag
      421 tttgttttcc ttcaaatttt ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg
      481 caatcgagct tcaatcaaat agcgactgct gatttcttta tcttgtagag aaataacata
      541 gcggttgtgc ttttgctgaa tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc
      601 tatcagtgct gctaattctt tcgtccgaat gattggcgga ccgatcgtta aaaagctatt
      661 tagatgagta aaccatcccc atagtatttc tttgtattcc ttagcagaaa aaacgtgcca
      721 ttcaatagcc tgccggattg gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa
      781 tgctcctgtg caattgcctt tttccgcttc ttttatctgc ccttcaataa atattcggct
      841 agcttctgaa aagggctcat tctgataggc aagaggagtt tctaaatacg accaatccca
      901 tctataaggg attagtgctg gatgctttcc gagacactcc tctgaagaga gggagagaaa
      961 atcatgctca aaatttttct ttggaatcgg caagtgatat tcttcgatga ttttcttgta
     1021 ataaaacaac tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc
     1081 cgactgatgc cattgctcca ggttttcttt tgttagtaac cgtggccatg ccattgctcc
     1141 gccttgcttt tgatttcttc ctcgagggta ataagggagc ccccttcttg atccactgta
     1201 tacaatcggt tccttgcctg atggatgata gaccagtttt cccttttcgt tctcgaagcg
     1261 ccctcctctt gatactgtaa acaatccgac ataatcaaaa aaacttaagc ctagacctct
     1321 taaaaagaca ttttctttag gcggtatctt cgaaagatct acatctgcag gattagaagg
     1381 aagctgatac agtaaaacat ttttctttgc gtagtctacc cacactcttt cttcttctgt
     1441 ctcatcattt tcccagtgac cgcttgctaa aacaagctga tcgatagaat agctttgctg
     1501 atctgtctgt aaaagataac catcttttct tttgcttact ttttttacta aagaataaac
     1561 aaactgaata gaagctggaa attcttcttg caactttgag aaaaaccagc gttgatagag
     1621 tccgtataaa catcgagtag attgctgatc tttttccaga cgattgattt cttctataat
     1681 tagttgctta tttttggtta ccgtctgttc gatataatct gtagcaggtc ctttactcca
     1741 ttgaaacaag ttgggtccct gtgttaccaa tcctccactg gacaatgttt catctgtaaa
     1801 aagcgtcact tgttgagaaa cagaattcat taataactcc ggtggctgat tcaaccgcca
     1861 aactcgacct ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg
     1921 attattatgg agaatccttt ctgctacact taatcctcta ggtccggcac caatgatccc
     1981 tattttcatt tgcatgcctc catttcgata aattttttca ataaaacagc atggttctga
     2041 tgttcatttt ttgcaccata aacaaaagta atgactggat gttctttcat aagttgactg
     2101 agtttaaaaa tagcttgctt cttttcttct tcgaaaatca attcttgctg gtatttcttt
     2161 gcaaatgaag caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc attactaggt
     2221 gcaatttctt tcaaccacag atctaactgc gctttttctt ttgaaattcc tcgtggccag
     2281 aatctatcca cgagtattct atagccatcc tctttttcag catcaaggta aacacgtttt
     2341 aatttgatca tagttgtttc cttctttcta ccttttcgct tagcttatat atgtagataa
     2401 tagcacaaaa taaaaaaaat gaacggcaca actatccatt gcgccgttca tgtgatcttg
     2461 gtcaaaatct aatgattgat tcggttttta aagctgtcat acaatcatct gttttatact
     2521 tttttacttt tgatttttcc agaccatccg tcatatccac ccttaagtaa ataaatctct
     2581 tgataacctg ctttacgtaa tttatttgct gtacggatgc tcaaactttt cttttgatcg
     2641 taaatataga caggctggtc ttttcgaata gatcctaatg aatctttcaa aacagtgtaa
     2701 gggatgtttc ttgcacctaa aatatgccca gcatcaaaca catctttttc gcgaacatcg
     2761 attacttgtg cttttctcat tccttctcga aattcttctt ctgtcaaagt cgttgctgaa
     2821 cgtttggcca ttactcgtag atagacttcc caaaggatga ttgcaagcac aatcaatagc
     2881 aaaatgatat taattaccca taaaaccatg taatgcctcc gtttttttaa gcaaatttca
     2941 aggctaatga ggcagcacca atgacaccag cttcattacc aagctctgct aatttgattt
     3001 ttgttgattc tcttacttgc gggaaagtaa attctttaaa atatttctct acacggctac
     3061 gtaaaaattc tcctgctgcc gatactcctc cacctaacac gatacttgac ggatttaatg
     3121 tattccccaa gtttccgcaa gctaaaccta agtagaagca tacgcgatca acaaccatca
     3181 atgcaaacgg atcgtcttct tgagccattt caaagatatc tttacttgtt acttcttctc
     3241 cgttgtctag cattgctttc agttttgaat cccctgcgta ttcttctgcc aagaaacgac
     3301 ctaggcgtac cacacctgta gcgctagata cagtttctaa acatccgcgt ttaccgcaag
     3361 tacattcgaa cccttcagga tccacagtga tatgtccgat ttctcctcca gctccgctca
     3421 cgccgtgaat caaatttccg ccagcaacga tacctccgcc aacacctgtt cctagagtaa
     3481 cgaatacaac gtctggatcg ttttctccag cacctttcca tcgttcacct aaagcagcaa
     3541 cattcgcgtc attatcgata gcgaatttga ttcctgttcc tgcctcgatc agttttttca
     3601 caaattgaac ttctgtccag tttaagttat acgcaccaat aaccgttcca gcttcgctgt
     3661 ccacgcttcc tggggtaccc atgccgattc cgataaagtc ttcggctttc atattatata
     3721 aattcaaacg atgattgatt gattcaatga tttcaggaac gatatgctta ccatcatcca
     3781 aaatattggt atcgatgctc catttttgct gaatctctcc ttctggtgtt aaaattgcaa
     3841 attttgctgt tgttccacct aaatcaatcc caataatctt cttgtccatt acaaatttcc
     3901 tttctttcgg ctttcttcaa ttcggtgctc tctttttaat atgcgccacg ctgtcatgta
     3961 tgtatcatga tcaatcaaac ggctatcata cagattacgc aattcgatag tcattaattc
     4021 aatgtcatag atgcgtgcac ccacatagat aaagatgcca aattttttga acagctgctg
     4081 tacatcatat aagctctcca tctttaagca cttccttcat attatacaag taaaccatat
     4141 ttttttaaac ccagtaaaat acatattaca aaaagaaata caaaaactag tgtagaaaga
     4201 atcctttggt gtatcgaata gcttgtctgt tgtttcggta gacccaaaac attccctaac
     4261 actaatccgc caattagccc accgacatgt ccccaaacat ctacagaggt atccagaaga
     4321 ccaaaaacaa atgtcaacac aataaataaa agaaaatgtc tgactagctg ctggatcgct
     4381 gggttatgtt taaaatgaaa ccctaagata aacaacgcac cgaacaaacc gaatatcgaa
     4441 gtacttgcac caccagacag aacattcgct tcgttaaaag caaagcttgc tgtatttccc
     4501 attatcccgc tgaacatata aatcaagaaa aaacgccaat ggccatagat tgcctcgatt
     4561 tgctgcccca taaaatacag tatcagtgag ttcattacga aatgcatcag tccaaaatgg
     4621 atgaagattg gtgtcactaa acgccaccat tcattgaagt gaacaagata aggaccatac
     4681 attccggcat aataagggat ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct
     4741 gtctgtatcc ctaaaaaagc ataggtccat aacggtcgtc ttaaccatgc acgcaatttt
     4801 atttgctgtt gataattcat gaaaacctcc catttcattt tagtcaataa acaattgttg
     4861 gatcttctga tcaaagctct ccggttgcca ttgttcattc aattgttcac gaaaaacaag
     4921 gctacatgtt tttttatgaa aattatttaa ataccggtcg taataaccac cgccaaaacc
     4981 gattcgataa ccggctctat tgtagataat acctggtaca atgaggagat cgatcatctc
     5041 cggcaaatag agcttctctg attgtggctc aagaacacca aaactcgaac gtacaagaac
     5101 ggactgttca tccatttcat aaaacgccat ctgacgtttc ggcaaagttt taggtacaac
     5161 tattttcttt tgttcttttt ctgctcgttg gataaccggc tgtgtatcca gttcaaattc
     5221 attcgataaa gtcgtcccta tcacttttgc tgacttccag tactcgctgg cgaatagctg
     5281 tttatagatt cgctgttctt tttccttttt cttgccactt ttggccagat ttttcagctt
     5341 ttcgattcct ttccggcgta atcttacttt caattggtcc aaaaactcac ccttccttct
     5401 ctcataaaat ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa taaatgcata
     5461 aatagaataa aaagaagacc tagaaatgat tggccttcct tttttaatga atttcaactg
     5521 ttcagcgcgt gtttttgtct gataaaaagt gcatacagaa aatatggcgc accaaaaagg
     5581 gcaatcaaaa ttcctgtagg tacttcccca cttagtagaa tcactctagc aaccatatct
     5641 gcactaagca gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca gttattttct
     5701 ttacgtacga ctagcttcgc agcatgagga gctagtagtc ctacaaaaga taaactacct
     5761 gtcaccgcta cactaccgct tgccaaagca acagctaaca acaaaaggat tttttgactt
     5821 ttagccacgt taaggcctaa tccttgcgcg cgttcttgac caaatgatag caatacaatc
     5881 ctctgtgcat aaagtcctcc tagcggaatt aagataaccg cccatggaag caatgcccaa
     5941 acataaggcc agctcgcacc ccagattgat cccgctaacc atgcattcac aaatccataa
     6001 ttttccttag ataattggat cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg
     6061 gcaataccgg acaataagat tcgattcata cccaaaaagc catttcgatc ataggccgca
     6121 agatagacac agcaagcagc taaaacacca cctagaaagg ctacaactag caatagaaac
     6181 ggcagcgaat agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc tgctccggca
     6241 ttaatgccca atatactaga atctgccaat tcatttcttg ttactccttg caagatataa
     6301 ccagaaacgc caagacaagc tcctgctaat aaactgatca gtaatcgagg aactcgaaat
     6361 tctctaacaa tcagcgccaa agaagatgat tctttcccta tgaacgtggc aaaaagctga
     6421 tccaggtaaa taaaaggcgt accaatcatt aaactaccta tagacaggat gacaagtaca
     6481 aaaagcaaga aatagaacgt ccaatatatt tttttcaccg ttgttccctc cttacttgta
     6541 tcagtaaaaa cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg
     6601 gattgatcat cctcgctgta aaatccgcca acataaccaa tccaccgcca actaaaaaag
     6661 agacaggtag aacataacga tagtccgttc caaccattcc gcgaacaact ggcggaacca
     6721 acagcccaat aaatgaaaca ggtccgacta acgaaacggc aatggctgct aacactgcaa
     6781 ctagacagaa aacaatcaac cgcgttcgtt taggatgact tccaagagct aatgcatgtg
     6841 tttcactcaa atgaatcaaa gatattgctt ttcctaatga aaaagaaacc aaaacagcaa
     6901 gtatatataa aggaaacaat actgctaatt gtttccatgt gatattggca gctccgccta
     6961 caaaccaaaa agcaagatcc tgctgcagat taaagaattg ggtaagtgct tggctgatag
     7021 cagtaaaaaa agaactaata gctacgccag ctaaaatcag ctgtaaagga ttcatgccca
     7081 ttttcgaaaa ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa cctgccaatg
     7141 acattagaaa cacagaaaaa tcagaagctt taggccaaaa agcaaaacta agggccattg
     7201 ccaacgaagc acccgcgttg attcctagta agcctgaatc tgctaaagga tttcttgtca
     7261 ctccttgcat caatgcaccg ctcaatgcaa aacaactccc tacaagaaat gctcccgcca
     7321 cacgaggcaa acgaatattt cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa
     7381 acaacgcctg ccaaaaatct gcttttccca cagtcgctgc gccatttgaa agagcaacaa
     7441 aaaaaataat caaaagaaca aaacagacca aaagcaggag tctatttttc ttcatagtcc
     7501 tcataattga tcctcctgct cttttggcaa caaatcatag gttagaaaaa ctggtttatt
     7561 tgttcttgga agcgtgacaa tcgttgcatc aatctcaaat atttttttca tacactcttt
     7621 tgtgatcact tcttcaggtg taccttctgt gatcaactta ccgtttttca ttgctaataa
     7681 ataatctgaa aaacgtgaag catgattaat gtcatgaatc gacatcaaga tcgttgtttg
     7741 tttcatttga ttgatttcag tcaataactg taggatctct aattggtgtg ccggatctaa
     7801 aaatgttgtc ggttcatcca agatcaagat atctgtttcc tgagcaagcg ccatagcgat
     7861 ccagacacgc tgggtttgtc caccagacag cgttgcgata tcccgcatac gaaatccagc
     7921 tagacctgta gcatccaacg cccactcaat tgcttcatga tcttgcgtgt ccatgccact
     7981 gaacagtttt tgataagggt atcgtccgta agatactact tcttctactg tcatacctgt
     8041 agggtgttca ctagtttgtg ctaataatgc aatttttttt gcaagttctt ttgaactgta
     8101 tgtagagata tcagagttat tgacaatgat ctttcctgat ttcacaggta gtatacgggt
     8161 aaaacttttc aataaagtag attttccact tccatttggc ccaatcaaac tagtgatttt
     8221 cccttcagga atcgtaacag acatgttttc aatgattttt ttgttttcgt agccggctgc
     8281 aatgtcagct gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa
     8341 ttatgacgct gtttcaacat ttcgtcaagc gctactttca tatctatagc caaatgaagc
     8401 ggttgacagc gttcgaaata tcaggaataa taaccaatga gaaagattct caattatgtt
     8461 aggaggaaag aaatgaaatt aaaaacaaaa gtattcacat tatctagcat attgctgctg
     8521 gctggagtac tgagcgcttg ctccccctct tcagataaag aaacagctac aactgccaat
     8581 tcgacgactt catccaatag tactcataca gtcacagata cacttggaca taaagtagag
     8641 attccaaacg cgcccaaacg gatcatcggc agttacttag aagactatct gattgcacta
     8701 ggggaaaaac ctatcgccca gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa
     8761 gatgatttaa aggatgtccc aacgatttct tatgaccttc cttatgagaa agtgttaagc
     8821 tttgagcctg atttgctgtt gatttcttct tccgctactg tcgaaggcgg gaaatacgaa
     8881 caatatagca aaatcgcgcc aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa
     8941 gaacaactaa aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt
     9001 tccgattatc aaaaatccat caagaaaact cgagaagaac tagcagataa aatcgagaac
     9061 aagacagctg cagtgttgtg ggtgaccaac aactcagcct ttatggtcag caaaaatcgg
     9121 tcaagtggaa gaatcgtata cgacgatctg gaatttggtg ttcctgatct agtggaagag
     9181 gtctccaaaa aagccacttc tgactggtct gctgtttcct ctgaaaagct agctgaatta
     9241 gatgctgact acatcatcct tgtgaacagt gacaaagatg ctgccatgtt caatgaacca
     9301 agctggaaga atctaaaagc agtgacagac aatcatgtac tgcaattcgg acctgaatct
     9361 agttggctat ataacggacc tattgccagt caggaaatgg tcaatgatat caaagaaaaa
     9421 ctaaattgat tttaactaaa aaaagcatct tcacgattag tcgtgagatg ctttttttct
     9481 tattaacaaa ctagacgcat agattttatt cttcttttga agcaacccta ttttttactc
     9541 tttcccattt ttgaaaacga tgggcatata gattttcttg atccgttatc ccctcatact
     9601 cctctataag ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg
     9661 tgctatcgat caatgttcgc cagatccgac cagtgtaagg aagaagttct tgaaaaatcc
     9721 gactaccacc cgaaacatag atatcttctg agatcgtttg actatacgac aaaagttcat
     9781 cgatcgaatg cattacttcc gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca
     9841 cgatgatatg gcgataagga agtgaaagat cccccattcc ttcataggtc tttcttccca
     9901 tcactaaaat tcgatccatt gtgtgttcgc gaaagaaacg catatcattt ggcaatcgcc
     9961 atggtaacaa accatttttg ccgatcagac catttttatc ttgtgcccac atggaaataa
    10021 acatattatc cgctccttat gattttagat gttttgagat aaaaaaagtc tcaattcttt
    10081 gaccagcaag gaattgagaa ctttaaaaac attattttgt ttcacggtgc aaagtaactt
    10141 tgcgttcgcg tgggcaatat ttttgttttt ccaaacgatc aggattgtta cgtttgtttt
    10201 tgcttgttag atagttacgt tctttacaag aagtacactc taaagtgatg tttacgcgca
    10261 tgtttttccc tcccattttt acaagaatcc tctaagatga ctctcagact tgattatctt
    10321 accatgattt tttctagatt gctagtgttt ttcacgattt tgttaagtaa aactacttga
    10381 cgataaatac aacggtttta cttctcaaac aaaatc
//
LOCUS       DACDHJ010000078        10308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDHJ010000078 DACDHJ010000000
VERSION     DACDHJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.080"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            90..359
                     /gene="rpsN"
                     /locus_tag="IV223_001503"
     CDS             90..359
                     /gene="rpsN"
                     /locus_tag="IV223_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8711797.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            374..553
                     /gene="rpmF"
                     /locus_tag="IV223_001504"
     CDS             374..553
                     /gene="rpmF"
                     /locus_tag="IV223_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8711798.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            585..962
                     /locus_tag="IV223_001505"
     CDS             585..962
                     /locus_tag="IV223_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711799.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            983..1132
                     /gene="rpmG"
                     /locus_tag="IV223_001506"
     CDS             983..1132
                     /gene="rpmG"
                     /locus_tag="IV223_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8711800.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1156..2139
                     /locus_tag="IV223_001507"
     CDS             1156..2139
                     /locus_tag="IV223_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8711801.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2169..2291
                     /locus_tag="IV223_001508"
     CDS             2169..2291
                     /locus_tag="IV223_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8711802.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2309..3832
                     /locus_tag="IV223_001509"
     CDS             2309..3832
                     /locus_tag="IV223_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8711803.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3856..4095
                     /locus_tag="IV223_001510"
     CDS             3856..4095
                     /locus_tag="IV223_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711804.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4288..4749)
                     /locus_tag="IV223_001511"
     CDS             complement(4288..4749)
                     /locus_tag="IV223_001511"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8711805.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4944..5162)
                     /locus_tag="IV223_001512"
     CDS             complement(4944..5162)
                     /locus_tag="IV223_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8711806.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5280..6896
                     /locus_tag="IV223_001513"
     CDS             5280..6896
                     /locus_tag="IV223_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8711807.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7137..8477)
                     /locus_tag="IV223_001514"
                     /pseudo
     CDS             complement(7137..8477)
                     /locus_tag="IV223_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8753..8908)
                     /locus_tag="IV223_001515"
     CDS             complement(8753..8908)
                     /locus_tag="IV223_001515"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8711808.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9204..9536)
                     /locus_tag="IV223_001516"
     CDS             complement(9204..9536)
                     /locus_tag="IV223_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8711809.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10050..10241)
                     /locus_tag="IV223_001517"
     CDS             complement(10050..10241)
                     /locus_tag="IV223_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8711810.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         3427 a         1648 c         2027 g         3206 t
ORIGIN      
        1 agcaactctt gttggtaaag tatcagtgtc aaaacgtaac gattacgatt tgaggtgtct
       61 aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa gtcgaaaatt gctaaagcaa
      121 agaaacaaat ggcgatgatt gaaaagtatg cagataaacg tcaggaatta aaagccgcag
      181 gagatcgcac cgctctcgct aaattgccaa gagattcaaa tccgaatcgt ctgaggcttc
      241 gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa gttcggtatg tcccgtatta
      301 agtttagaga acttgcccat caagggttga ttcctggagt aaaaaaagca agttggtaaa
      361 aggaggaaat atcatggcag taccagcaag aaaaacatct aaggcgaaga aacggttaag
      421 aagaactcat caaactgtag taaaaccaga aatttcattt gacgaagcga atggtgatta
      481 tagacgtagc catcatgtat ctttaaaagg gtactataat ggaaagaaag taattaaagg
      541 agcttcaaaa tagtttaata tataactttt aggagggaat caaaatggaa attatttacc
      601 cacctttagt tgaagaagga ttaaagtatt atcttgaaac gacccagcaa tcgctagata
      661 aatgcacttt ttatcgttcg atggtagaga gaggaattat tactgaaacg ggtttaccga
      721 cccaacaggc aattgaaaat ggattagtca aagattacta tgaggaccaa ggcttatcat
      781 ttgatgattt tttaagaatc tatccaattt ttgaagagta tgacgaggag ttatttcaat
      841 gtatcgatgg ttattgggag atacctgttg atatgaaaga aaatttagtt tcacaattgg
      901 aatctggcga gttaactttt gaagatgcac aacaaattca agcatatctt gaagatcgat
      961 aagtattgaa ggaggaagat ttatgcgtca aaatattatt ttggaatgtg tcgaaactgg
     1021 agaacgtctg tatctcacca gcaaaaataa acggaataac cctgaacggt tagaattaaa
     1081 aaagtattcg ccaaaattgc gcagacgggc aatttttaaa gaagtgaaat agctaagtgg
     1141 taaaaaggtg acaaaatggg cataccagtt acaatcgttt ctgggttttt aggggctgga
     1201 aaaacaacgc taatcaatca agccttgcaa gtctcaccct accccaggga agaaattatt
     1261 ataattgaaa atgagtttgg tgaagttggg attgatcaca aattattagt tcatagcgag
     1321 gaacgggtgt tacaactaaa taatggatgt atgtgttgca gtcttcgcag tgatttatta
     1381 gcaatattga tttctttact ggaaacaata gaagaacatc aacaaccaat ttcacaaatc
     1441 atcattgaaa ctacaggtat agcagatccc caaccaatca ttcaaacact tttaacagcg
     1501 cctcaactaa gaggaagagt atatatcgat agtttattga ctgttattga tagtgacagt
     1561 tttgaactta ttgaaagaga accacaagcc cttaagcaac ttgtcatggc agatcgcatt
     1621 tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa aaaaattaca gagagaaatc
     1681 aaaagtatta accctttatc cgatattcga atgttcacac atagtgaaac gattcaaaaa
     1741 aaggattttt ataatttgaa aaaatttaat catcccttag tttttgaaag tgattcatca
     1801 gaaaatcact ataatggcaa atactatcat caacaccatc aagtacatga gtttaaatca
     1861 attctcttaa cttcagatca agacataaaa aaagattttt tactccaatg gattgactgg
     1921 ctgattttaa ccaaccaagg gagcctgtat cgttttaaag gattgattgc tttacaagag
     1981 caggatttta tagttgccat gcaagaggta aacgaacagg ttaattttca atacaccact
     2041 cgacaaaaag accaaaccac tattattcta atcggaaaaa tgttggatga agaaaggatc
     2101 aaacagagtt ttgataaatt actggcggaa agcaaataat gataagtagt gattgggggt
     2161 aatttctaat ggagaaaacg gatatatcta gtgcatatcg acgacttaaa agcccgaata
     2221 ttaaaactag aaaaagggca ttaaaaatta tcaaagaaca taaacgaaat aaaatgaaga
     2281 aattagcata attaaggaag gaaaaagaat gaagaaaatc agtgtaggac ttattgggat
     2341 agcagctcta ggattattgg gagcttgctc cagcactaat gatgcaaaag tatcgaatga
     2401 taaagatgga aaattagaaa ttgtaacaac attctatcca atgtatgatt tcaccaaaaa
     2461 catcgtcggc gatgaagcga acgtggattt aatggttcca gcgggatcag aacctcatga
     2521 ctatgagccc tcagctaagg atatggcaaa agctcatgat gctgatgttt ttgtatatca
     2581 taacgaaaac atggaatcat gggtaccaaa agctaaagaa agttggaaga aagctgggcc
     2641 taatgtagta gaaggaacaa aagacatgat tttattgcca ggtagtgaag aagaagatca
     2701 tgatcacgga gaagaagatc accatcatga actagatcct catacgtggg tttcacccaa
     2761 aatggcaatt aaggaagttt caaatatcaa ggatcagtta gttaaattgt atccgaaaaa
     2821 agcaaaagta tttgagacaa atgctgaaaa gtatttgact aaactgaaac ggttggatgc
     2881 tgactatacc actagtctta aagaagcgaa acaaaaaagt tttgtgacgc aacacgcagc
     2941 ctttggatat ttagctttgg attatggatt gaaacaagtt cctattgctg gactaagtcc
     3001 tgaagaagag ccatcttctg gtcgattagc tgaattaaaa gagtatgtta aaaaaaataa
     3061 gatcaattat atttactttg aaaagaatgc taatgataaa attgcaaaaa ctttggccaa
     3121 cgaagctggg ataaaattgg aagtactgaa tcctcttgaa agtcttacaa aagaacaaat
     3181 ggataatggt gaagattatg tttcagtaat ggaagacaat ctaaaagcgt tagaaaaaac
     3241 aactatggtt gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg aaaagacagt
     3301 tgctaatggg tactttaaag atgccgatgt gaaggatcgc gaattgagtg attatacagg
     3361 cgaatggcaa tctgtttatc cgttgttaaa agatggaatt ttggatgaag ttttcgatta
     3421 caaagcaaaa atcaataaag acatgacagc tgccgagtac aaagattatt atactactgg
     3481 ctataagaca gatattgata ctatcaacat taaagacaac accattgatt ttgtagtcaa
     3541 cggtgaacat catcaataca catacaaata tgttggatac aaaattttga attatgaaaa
     3601 aggaaaccgt ggtgttcgtt ttaattttga aactgatgat gcaggtgctg gtcgttttaa
     3661 atatattcaa tttagtgatc atggaattgc accaagtaag gcagagcatt tccatatttt
     3721 ctttggtgga gaaagtcaag aaaaactcta taatgaattg cataattggc ctactttcta
     3781 tccagcttca ttgtctaaac atgaaattgc tcaagaaatg atggctcatt aaaattcaat
     3841 ttttggagga aatacatggt aagaaaagta ttattgtcta tggattccaa gaatattgat
     3901 gcttatcggc gttatcgatt tgcaaagcaa atgagattaa atgggagtac attagctgat
     3961 attgctgcaa ctttacatgt tagtattgac acagttcata gagtattaaa acgggatcct
     4021 gattgttgtt ttatcaaggt caatactgga agaagtagac gtttagcaaa acggcataat
     4081 agcttggcca attaattgat aataccagag tagatcattt ttcccttatt tggatttgat
     4141 ctgcttttta ttatgtcttg aattgtcaaa tcaagcgcaa cactttatca aagaagactt
     4201 cataaggtgt tctccaatct aaacactttc taggtctgtt attcagtttt tcaatcatct
     4261 gatttatcgt tgagttatct accgtagcta tctctttcga ttttggcaag tattctcgaa
     4321 tgagtctatt cgtattttaa tttgttcatt gttgccaagg gcatgaggat ctggaaaata
     4381 aaagggcata ttattcaatg cttctgtaac tgcgaaatga ttggagaatt cttttcctct
     4441 atctggagtg atcgttttga ccgcctcatt tggaatactg cttaaaagtt caatcatttt
     4501 ctcgctcaca taagcggctt tctttctggg gaccttcttt cctgctaaaa ggaagcatga
     4561 ttttctgtct gtcaaagtca ctaaacaagt accaccagtt ttgccagcta caatatcggc
     4621 tttccaatga ccaaaatcac ttctatcatc tgcggatttt ggacggccat ggatcgaatg
     4681 agtaaggcaa atctttccac gagactcgac atgatttttt gtatgtcttg tttttcctcg
     4741 atggtgcaat gaacggatca ggcctttatt gccgtgtgag aggggttctg tatcgaaaag
     4801 atcattgcaa attgcacgtt agatcgtgat aaaactaatt gaaaagtcca tatgttcatg
     4861 cttcaaacga ttcgagattt cctctggcga ccactgaaaa tcgacaaata aatgacgaac
     4921 aattgaccaa actcacggat catttagaat gtgataacgt ccgcattttt ttcttcgata
     4981 agcataattt ttttccgctt tggaaggtga atagtttttt tcttgtttct tctcagttca
     5041 tgtaaaatag taaacgggct atgctgaatg tcgttgccaa ttatactaat agaaaatcct
     5101 tcttcgtgaa gcagaaagat tctttctcgt tcgagtatag taagatgacg atactggctc
     5161 ataattattt tccttctagg agtgttctcg acaaacttat actagttgga tatcgattat
     5221 gactcatttt tatgtgttgc acttaaagtg taaattcaag gtatagaaag aggttcccta
     5281 tgaaaaaaat acctattaca atcttaactg gctatcttgg ttcaggaaaa acgatacttc
     5341 taaatcatat tctgtacggt gatcacggaa aggaaattgc tgtcattgta aatgacatag
     5401 gggaagtaaa tgttgatggg gtattagtcg acaagaaagg ttttaaaaga actggagaaa
     5461 aacttgtttc gatgccgaat ggatgcattt gttgtacatt acgcgaagat ttgattgttg
     5521 aactagatca attagtgcaa ttttctgact tgaatcaaat tgttattgaa gcttcaggaa
     5581 tcagcaaacc aattccaatt attcaagcaa ttatattagc taaaaaccct ttagacattg
     5641 atttgaccca acgattagag attggtgcca ttattatagt agtggatgta aaacgttttt
     5701 gggataattt cgaaacgggt gaaaagattc aggaaagaaa agctgaaggt aatgtagttg
     5761 aagaagaaca ggatattcgt ggcttgttaa ttgatcaatt cgagtgttgt aacatattac
     5821 ttttaaataa tagtgactta gtgtcaacca agacgattta caaaattaac ggtctaattg
     5881 ctaagttaca gtccaatgcc aaaataatta ggacaattaa aggtgaagta gacttatcga
     5941 aaattctaaa tacaaatctt tttaatatag atgagacttc ctattcagtg ggctggttca
     6001 aagagttaga gcttggctat caaaatcata ttcctgaaac agaagaatat ggtatctcaa
     6061 gttttatcta tcgaaatcgg ttactccttg acgcacaaaa atttgcccat cttacggata
     6121 aaaattttcc tggcaatatt acaagagcaa aagggtttct ttggtttgcc caacatagtg
     6181 atttttcgac gatctttgaa acatcgggta gctataaaag tattgaatca ttaaattatt
     6241 gggtagctag tttacctaaa attgagtgtc aaatgattat acaaaaaaat cccgaattga
     6301 aggaagcctg gcatccgttg tttggtgaac gcattaataa actagttttt attggtatga
     6361 agatggatca acaatgtatt aaggaacaac ttgagggttg tttaactgat tctcttaatg
     6421 cagataattt ccgaaaaatt tcaccatttc catggaaaaa gtctaatatg tttagccaga
     6481 tatcgttaat gaaattttta agaaaagctg gtgtcaagat aggcgatgtc ataattattg
     6541 gcattttgat tattacctct tttattccct tatttgtatt ctctatagaa aaggagaatg
     6601 aaacaactga agatgctatc aaatatgcca tcgttcgaat tgatggcaag gaaatggatc
     6661 gcttcgattt ggataagatc aatcataaac tcgtgaatta tcatcctgcg aagggacaat
     6721 ataatattgt tgagattaaa gatggaagaa ttcgagttaa agaagataat agtcctgatc
     6781 aaatcgcggt tagaactgga tggatctcaa aacccggtca aacgagtatt tgtttacccc
     6841 ataagttagt tattagtatt gagaaaaaag aaagcacaga ttactacatt tactaatctg
     6901 gaaatagatg aggaggtttt taataagaaa aagatattat tattaataat agcaggggct
     6961 ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac aggcacaagt
     7021 atctttaacg ggacctgcaa tggtttagtt aacagtcggt tagattactt attttggctc
     7081 ttcgtcaatg agtgttggtg aagaaattca atagaataat cgagatactc acacggctag
     7141 acagctatct tgtctagccg tgttgttgtt ctggctttag tcagttgggt agtattctta
     7201 gttttcttaa aatattttcc tcgatataaa aagattcgtt ctcggtagtt ttgaaagttt
     7261 ctatagccat aggcaattcg ttttagtact ttgatatggt tattcaaaca ttctataggg
     7321 ccgttggaat agggaacgcg caacgctcgt ttgatttctg tttggtattt cttgaaggtc
     7381 gtgaacactg gttggtagtg ctctggcaac tccttgtagt cttcttttag gagcgcgacg
     7441 aattcaggaa catcttgtct tctgataacg gaaagaaaat cctggtagac ttcgtagcct
     7501 cgctttaagg aatcatcata agataatagc cgatcgacta tctctgcttc tgtcagatga
     7561 tctcgaaatg acggacgcca gtagcgatgc tgacctttca attcccacgc attcttttga
     7621 agtagtttcc agtatttctt taagtgcttc gaccgacgat cccctttttt aaaggtcttc
     7681 attacttgaa ttcggtggtt ggtaaaagcc cgaccaatgt gttggctaat atgaaagcga
     7741 tcggcgacga ttcgggcatt cggaaaacac tctttcacca atgtgcgata gggtgtatag
     7801 aaatctgaaa ccactagacg cacttgttca cggactttta tagggtagcg caagaaatag
     7861 tcgcgtaaag gattgagttg ccgattttcc acaatatcaa tcagttgatg ggtcttgccg
     7921 tccatcatga caaagctcat ggctcccgag acatttttca ctgatttgaa ttcatcgaaa
     7981 cacaccactt ctggtaacag ctggctcaaa gaaatggttt taggccgaag gctgcgaagt
     8041 attcgaatga cagtggtcgg agaaatgtgc ttcatacgtg cgatcaaaga catagaaaga
     8101 ggttcgctta atagctcaag aatggcttgt ttgactctgc gagcaatgga gcagcgacga
     8161 tcactaactg aatcttctgc taaaaaagtc tttccacagt gtttacattt gaagcgctgt
     8221 ttgttaatcc gcagaatggt cttgtagtta ctgacatcat tcaataagat ggatactttt
     8281 ttccagccct atttaatgat cgtttgttca ttttgatagt ggcgatgaaa gcattcctcc
     8341 ggttgatagg tcaatgtgcc atcaaaaaca aaacatgttt cgtcattgat tcgctctttg
     8401 cgaaaggcat cttcatgaaa gataagattt aggtctagaa tatctagtaa ctctttggta
     8461 aactctgaat aggacatctt gaaacactcc tttgaatggt tttcgtcgac tttattctaa
     8521 aggattttaa ggtgtctgtt tgcgtttatt agaaaaaaga aaaagtattg gcaacagaag
     8581 gcgtcaccaa cactagatat tatagatccc ttattttctt cgcttcaaat tgtcaaggag
     8641 tcatcataaa ttttgtatgg attcttttgt ttgggtagta gtcttctaaa taatcttgga
     8701 ttaacacctt catctctatt ttacttttat atttgttgga gtcgattaat tctcatttga
     8761 ttgatcgatg aaacgcttca attactgcat tgtcaacagg agtcccagtt tggctcactg
     8821 aatgtgtgat gtggtggtcg ttcaacagct cttgataaat aaaagaccga tgctggctac
     8881 ctctatcgga atgaattacc gtaggcaatt ctccttaata aatttgtaat ggacggacga
     8941 caatctcttt tttcatgtgt gaatctacca tatagtaacg tagaatacga gttgctaagt
     9001 caatataggt ggacaaatag aggcgaccgt cattacatgg aatgtaagta atatccatca
     9061 cccaaacagc atcaatttgg ttctgttcaa attatcgatt caacattttt ttaccatttc
     9121 tttgattgtt ttttctctac catgagaaga tttgcgtcga tgaaatcctc ttgtgcataa
     9181 attcatttca cgcataagaa cagctacaac acgtttattg atttcgattc cttcatcgta
     9241 aagtttttga gtgatccctg gagatccgta tcgatctttg tgttcgtaga acactcgtct
     9301 tcctctttta gtcgtttatt ttcctgttca agaatgtaga aatctgcttt atcaggtaat
     9361 ctttttcctt gtccagcaaa agcatttttc ccgtatcgac gatattctga aacccatcgg
     9421 gaaattgttg gacgagagat attgtattct tcagcaattt tcttggtagt tacttttttc
     9481 tcaaggatat cctgtgaaat ctttagttta aatttacgat catatgtttt tctcaataga
     9541 gttcattcct tccggtaacg taactgatag tcaagaaaag tgtatcatag cccaacttag
     9601 tatcagggtt tgtcaactaa actgtggaag ttgatagata agagttttta acaaccaaaa
     9661 ttctcttgac tatttttgaa atcagacaaa ttttgattgg acacatagtt gaataaatct
     9721 accgtcgtta cggaaggtaa atcgcatgct tttcaattct agaggagaag tcctcgttga
     9781 agtaccatag cccagttctg gattcgacct cctgcccact tcatacgtaa ataaagtaat
     9841 tttaggagtg agactgtgac aaaagttatt tttaagctaa aaacgaatta aaaaacgttg
     9901 ataattaaaa tcattcgttt tagattctat gaatcattat agaacgctgt aaaatcgata
     9961 ctttataatt ataaagaaga tagtttcaaa tttattgttc gaaatttgaa actatctttt
    10021 tacttatgtc acagactccc ttagttgagt tattctttaa tttcgaattt cgagatagct
    10081 agatatattg caagcataca ttcttgataa caatcttgat catattttcc agttctccag
    10141 gattgcttat gaatcaaaga gctaaatctt ttgagaattg cctcaacagc ttcttcattt
    10201 ccagcctttg ctaaaatgaa atcttctttt aaagaattca taaatctacc ttcttcccat
    10261 ggacttataa tctatttctt tgggaaggag gagaacgagc aaagaagc
//
LOCUS       DACDHJ010000079        10222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDHJ010000079 DACDHJ010000000
VERSION     DACDHJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.081"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(305..1528)
                     /locus_tag="IV223_001518"
     CDS             complement(305..1528)
                     /locus_tag="IV223_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8711811.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(1559..2398)
                     /locus_tag="IV223_001519"
     CDS             complement(1559..2398)
                     /locus_tag="IV223_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8711812.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(2415..3095)
                     /locus_tag="IV223_001520"
     CDS             complement(2415..3095)
                     /locus_tag="IV223_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711813.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(3097..4170)
                     /locus_tag="IV223_001521"
     CDS             complement(3097..4170)
                     /locus_tag="IV223_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8711814.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(4817..5488)
                     /locus_tag="IV223_001522"
     CDS             complement(4817..5488)
                     /locus_tag="IV223_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8711815.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(5575..8253)
                     /gene="mgtA"
                     /locus_tag="IV223_001523"
     CDS             complement(5575..8253)
                     /gene="mgtA"
                     /locus_tag="IV223_001523"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8711816.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            8912..9349
                     /gene="ndk"
                     /locus_tag="IV223_001524"
     CDS             8912..9349
                     /gene="ndk"
                     /locus_tag="IV223_001524"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8711817.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(9371..10000)
                     /locus_tag="IV223_001525"
     CDS             complement(9371..10000)
                     /locus_tag="IV223_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator, Rgg/GadR/MutR family
                     domain-containing protein"
                     /protein_id="HAP8711818.1"
                     /translation="MLDRVQEDSFDPYKEISNNRRHFKKTEDLYYLVLNLHMVEKTEV
                     SETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFPWIFSDRFIENNYLKIKKRMRR
                     LAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNEDMKRSLAHKERSTVIYETLMAD
                     YYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSLEKKLLAIAEPSN"
BASE COUNT         2880 a         2232 c         1738 g         3372 t
ORIGIN      
        1 aaaaagacct gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca
       61 gatctgtttt tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca
      121 attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac
      181 tgcgtgaaaa gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa
      241 gaaaatattt tttcattttt ctgaaaatta tttttataac tttcacacac aagtttattt
      301 tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct
      361 tgattctttc taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt
      421 ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt
      481 ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga
      541 cttggctgac atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt
      601 cttcatctgg aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag
      661 taacgatgac atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac
      721 ttatcgcagc acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc
      781 gatttcctgc acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta
      841 ctcctccgtc tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag
      901 cgttgattcc attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg
      961 ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag
     1021 attctgtcgt tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct
     1081 caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc
     1141 ctggggtgcc atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc
     1201 tggcggctaa tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg
     1261 gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac
     1321 gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt
     1381 ctaattgttt aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt
     1441 cgtggatatc tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca
     1501 attggtttgt tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt
     1561 attttctccc gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta
     1621 ctttgactgt gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt
     1681 catcttcttt tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt
     1741 ctaagaagat ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga
     1801 tcactgaaat gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa
     1861 tgtctagatt ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct
     1921 tatccacttt gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa
     1981 catcattagg gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt
     2041 tagaataaat tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt
     2101 gttctttatt gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc
     2161 catctgctaa agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa
     2221 tgttctcctt tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa
     2281 tccctagttt tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc
     2341 ctgccaacca aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt
     2401 tttctcctcc actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt
     2461 aaaaaaacta agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga
     2521 ttataccctc tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg
     2581 gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa
     2641 ccttctaaca aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc
     2701 gcttcgatca ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac
     2761 ggaacaactc caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact
     2821 aagaaacgag tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg
     2881 atattgatca gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg
     2941 cgtgtcacta ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc
     3001 caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa
     3061 acattgggca aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt
     3121 gatacgacta tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa
     3181 ataatccaat gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc
     3241 taacggcgtt tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata
     3301 tttgctgtac agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc
     3361 caccagccat ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa
     3421 tgcttggtca agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt
     3481 gctgaagatc tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac
     3541 cttgttgcag atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc
     3601 taactggcga ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag
     3661 tgcactggtt gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat
     3721 tgctactcgt tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc
     3781 caaaccgaca agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc
     3841 agaatacttt aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa
     3901 atgctgaaag atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg
     3961 actcagattc gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat
     4021 cacacgaact aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc
     4081 tttttcaatc gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa
     4141 acatacagaa acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc
     4201 ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg
     4261 ttcaagctgt gttcacacac aactcctctt caagtacgat catactctag caagatatcg
     4321 gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc
     4381 aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg
     4441 tacttaccat ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta
     4501 tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa
     4561 gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt
     4621 gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc
     4681 tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga
     4741 tattttgttg ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa
     4801 attatttttt ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa
     4861 gatcttttcc ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga
     4921 caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc
     4981 ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag
     5041 ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg
     5101 tgtccaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag
     5161 tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg
     5221 catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct
     5281 tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac
     5341 tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa
     5401 attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac
     5461 catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca
     5521 tagtttagca cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa
     5581 gagttcttgg aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc
     5641 gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc
     5701 agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac
     5761 aaacatggca aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg
     5821 gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat
     5881 gaaaatcact cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca
     5941 aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa
     6001 gtaaaccatg aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc
     6061 tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa
     6121 caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga
     6181 aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa
     6241 agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat
     6301 cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc
     6361 cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt
     6421 tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc
     6481 atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt
     6541 aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt
     6601 gtccccagtc agaactttta cagctacacc atgttctttc agagctttca acgcttgttt
     6661 cgttgtttct tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc
     6721 atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac
     6781 tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt
     6841 tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc
     6901 aatcgcgcct tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat
     6961 acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt
     7021 atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt
     7081 ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca
     7141 atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca
     7201 taacacatcg attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt
     7261 tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc
     7321 aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt
     7381 tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag
     7441 catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt
     7501 ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact
     7561 tccactgatg acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg
     7621 atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc
     7681 ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc
     7741 tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt
     7801 tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg
     7861 attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat
     7921 aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa
     7981 tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat
     8041 ttctacaatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct
     8101 tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa
     8161 ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc
     8221 catctcaatc tctcctttgt gtccaaaaga cagctgttct cgttgctttt ggtctttagc
     8281 tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc
     8341 tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct
     8401 ttactaagta attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt
     8461 agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc
     8521 caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt
     8581 tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa
     8641 gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag
     8701 cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata
     8761 acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata
     8821 cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata
     8881 atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc
     8941 agacggcgtg agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact
     9001 ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca
     9061 gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt
     9121 cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc
     9181 aacaaaagct gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac
     9241 tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt
     9301 tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta
     9361 aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc
     9421 gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct
     9481 tttcttcccc taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat
     9541 agatcactgt acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac
     9601 gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa
     9661 agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca
     9721 aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata
     9781 gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat
     9841 aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca
     9901 aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt
     9961 tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact
    10021 ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc
    10081 gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc
    10141 cgactagaga agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt
    10201 tgactcgaag tttcttgatc aa
//
LOCUS       DACDHJ010000080        10077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDHJ010000080 DACDHJ010000000
VERSION     DACDHJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.082"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..572
                     /locus_tag="IV223_001526"
     CDS             66..572
                     /locus_tag="IV223_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8711819.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            589..1488
                     /locus_tag="IV223_001527"
     CDS             589..1488
                     /locus_tag="IV223_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8711820.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1575..2459
                     /locus_tag="IV223_001528"
     CDS             1575..2459
                     /locus_tag="IV223_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8711821.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2651..2722)
                     /locus_tag="IV223_001529"
     tRNA            complement(2651..2722)
                     /locus_tag="IV223_001529"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2687..2689),aa:Arg,seq:ccg)
     gene            2782..4116
                     /gene="rpoN"
                     /locus_tag="IV223_001530"
     CDS             2782..4116
                     /gene="rpoN"
                     /locus_tag="IV223_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8711822.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5356
                     /locus_tag="IV223_001531"
     CDS             4319..5356
                     /locus_tag="IV223_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8711823.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5397..6398
                     /gene="gap"
                     /locus_tag="IV223_001532"
     CDS             5397..6398
                     /gene="gap"
                     /locus_tag="IV223_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8711824.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6508..7698
                     /locus_tag="IV223_001533"
     CDS             6508..7698
                     /locus_tag="IV223_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8711825.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7802..8557
                     /locus_tag="IV223_001534"
     CDS             7802..8557
                     /locus_tag="IV223_001534"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8711826.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8673..9971
                     /gene="eno"
                     /locus_tag="IV223_001535"
     CDS             8673..9971
                     /gene="eno"
                     /locus_tag="IV223_001535"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8711827.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3526 a         1860 c         2096 g         2595 t
ORIGIN      
        1 taaaaaaatt gaaactttta gcgcaccttg tacgtcatat taagtgaggc agaaaggagt
       61 ggagaatgat ttttactgga tataagaaaa aacggcagat cgaaaaatac cgtcacctgc
      121 tgaatggact catcgaaact gattatcaaa aaatgttccg tatcacgatg aattacgtac
      181 atgataaaga agaggcctta gatgtgatgc aagatagttt tcataaagcc ctgaactatt
      241 ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt ttatcggata ttgatccgca
      301 cggcattaga tagttggcgc agacaaaaaa ggacgcctgt ggacttattt gaaattcaag
      361 atatccccca acagttacct ttagaaactt ccgttgcaga gctccatatg attttagata
      421 aaatagacag tccggaaaaa gagattctga tcctctactt ttttgaagga tttcgtttga
      481 aagaaattgc gactatcttg gatatgaatg aaaacacagt taaaacgaaa atgtaccgct
      541 cattgaatgc tttacgtcaa atcctagaat gaaggagaga agagtgccat gtattcccaa
      601 aaagaaaatt taaaaaaact aaagaaaact tataaagaac aagcaattcc caaagggctt
      661 aaagaggaga tccgccagcg ttttattatc gaagagcaat catttataaa agcaaagcgg
      721 agaaagagac gtctccaagg gttcggagta tctttggcta tcgtaatgat tacaacaagt
      781 agccttcttt ttaatagtca agttcgaagc tttgcagaag atttacccat cttaggatca
      841 gtcattgaat tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat cgatattcag
      901 gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa caattcatgg attaaatcaa
      961 aaatactttc gcgaaggaca agcagatttt gaaaaagctg aaaaagaata tggaaatttt
     1021 gaaacggatc attaccaagt tttgggagat tatcagaaaa tcgtagacga taaccggttt
     1081 ctcgtgattg aaaggcagat cacccaaaca gcagcagata gccatgttga aaaaagatac
     1141 gacacaatcg acaagaaaaa cagtgtccag ctctcacttc cacttttatt caaagatgac
     1201 acctatctat ctgttttgac aaaagaagtc aaacggcaga tggccgaaca agtcaaggaa
     1261 gatccatcta agtactattg gactgagcaa gatattcaag aagggacgat agaaaaacca
     1321 acacttgtta cgcctacacg aagtttttat ttgaacaaag agcatcaatt agttttgact
     1381 ttttctcaat acgaaattgc tcctggttat atgggtacac cagaatttgt cattcctaag
     1441 tccgtgacaa aaacgatttt ggcatcggaa gattatttgg atcattagac gagaagtagt
     1501 tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa taataagaag agaaaaagag
     1561 aaagaagaga tatagtgaaa agaaattatt tagttatcag tattattgct ttatccgtgc
     1621 ttttgttttt cgaaattact tcggtggcct ccttgatcac cgaagtaaaa ggacaaacac
     1681 actcttcgga gcattcgtca caatctttca aacaatcaga acaatcaacc attaaaagcc
     1741 aatcaactga aacagctaac gtcaaagaag ccattcaaga atcagcggaa aaggtacaaa
     1801 gtcagtcatc acaatcaaca caagacagta caaaaacgag ccgagatccg gctgtcaaaa
     1861 gcgtcgcaat cagctttgat gatggaccag gagcgaccac aacaccgcag cttctgcgaa
     1921 tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt aggagaaaat acggcgcagc
     1981 atcctgaaat cgtgaagcag acagctgaag cagggcacga gattggcaat cacacatacg
     2041 atcatcagaa tctggccact ctttctgccc agtcgatgac ggaggaagtg acgaaagcag
     2101 acactgagat caaaaaagtc actggcaaaa cccccacttt tgttcgtccg ccctatggtt
     2161 ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt
     2221 cagaagattg gaagacaaga aatcctgatt tgattttgca gaagatccaa gcaacagtat
     2281 acgatggggc aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc
     2341 aagtgatcga ttatctgaaa gagcaagggt atcgcatcac gacagttggg gatttattag
     2401 ggcatccaac ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag
     2461 tgagacaagt atctttaata aataacacat tttaataagt agttgaacac ataactacct
     2521 tacttgcaaa ggcttaacaa tatctataca ggtacaacaa aaaactgtcc accaattttc
     2581 tttggcggac agttttcact taaaaataaa gaagcaacta ttcgtaaatt atgctaaaat
     2641 agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga atcttacgtg
     2701 atatccacta cactaaggac gcaagcactg ggataattgt aactaaacag ataaaaaatt
     2761 acaaggggat tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa acagcagcag
     2821 acacagaaac tagccatgac gcaacagttg caacagtcca tccaagtttt acaatataac
     2881 agtgaggagc tgttggcatt cgttgagaat caagcaatgg aaaatccatt ggtggaagtt
     2941 gttgagccgg aatggcagcc agattacata aaagcttctt catcttctta tgaaggagaa
     3001 gagacaaatt acctaaatca gattcctgat acaaaagggt ctttatttga ttcattgatc
     3061 gaacaagtcc atttgaatta ccgagatact tttttgagga aaatcgttct ttatcttgta
     3121 gaatacatcg atttgaacgg atttttgacg attagtttag aagaagccgg aaaagagatt
     3181 ggagctacgc ctatccaaat gctcgatgcg ttgactctga ttcagcaatt agaaccagct
     3241 ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg agatgattat
     3301 gcgccggaat tagcttatat cgtgttggaa gaatgttttg aggaactggt ggagcgaaaa
     3361 tggaaagaaa tcgcacaacg tttttctgtt gatctacatg ctgttcagca gatattcgac
     3421 tatttacaaa cgttgagtcc aagccctggc cggattttcg accgtagcag cgaattgtac
     3481 attattcctg atgtccgggt attagtggac gacgataaaa atgtacaagt tatctcaaac
     3541 cgaaaaaatc agccaaatat acgctttcaa gagagttact tcaaacaaat gtcacaacaa
     3601 gcagataaag aaactgaaaa ttatttaaaa gaacggaaac aggaattcga atggttgaag
     3661 aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat tgtttcccgg
     3721 caaaaagctt tctttatcga caaggaacgt ccaatcaagc ctcttacgtt aaaggaagtt
     3781 gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg gaaatatttg
     3841 gaaacagact tcggtgtatt tgaattaaaa aaatttttca ctacacgaat tcctacgaat
     3901 agtacagaac agacagaaga cctttcagca gatacagcga agaaaaagct gcaggaatta
     3961 gtcgatcaag aagacaaaaa caagccgttg tctgaccaga aattggttga attgctgaaa
     4021 aaagatgaga tagcgatctc aagacgaaca gtagcaaaat atcgtgattt attaggaatc
     4081 ccgagttcat caaaaagaaa acgttatgat aactaatcag tcaaaagact agaaaaagta
     4141 tttcactttt tctagtcttt ttagttgaaa aacgagagca aaactgttag acttacagtc
     4201 gtgaggttga gatttccctt tattgatgtg aaattcgaat agttaatttc tttttttatc
     4261 gtatatgggt cgttttaagt ctacattgga cgtaaagtgt ccaactaagg agatcgctat
     4321 gctggatgaa ataaaaatga ttgaatctgt tgcaccagat attatcgaag tgttgcaaga
     4381 acgctataaa attttacgaa atatctattg gatgcagccg attggacgtc gaagtttatc
     4441 ggaaagtatg gggatcacag aacgagtatt gcgaacagaa acagatcttt tgaaaaatct
     4501 gaatttgatc gacacctcaa aaagcggtat gacgctgaca actaagggcg aagaagttta
     4561 tcaaagcctt gaaaacttca tggatcagct tttaggtacg catcaaaccg aacaacagtt
     4621 ggcagaatat tttggtaacc aacgctgtat cgttgtttca ggaaacagcg aagagcagac
     4681 caaagtggct gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac tacttccaga
     4741 aggcgagaat atcatcgctg tgatgggagg aactactatg gctgtggtag ccgaacaatt
     4801 gtctaatctg gaaaataaga aacggcataa cctgtttgtc cctgcaagag gcgggattgg
     4861 tgaagcgata actgtacagg caaattctgt cagtgcaaga atggctgcta aagcaaacgg
     4921 taaccaccgt gcactttatg ttccggaaca attaagtctg gcaacttaca attctttact
     4981 caatgagcca tctatccaag aagtcttaaa cttgattagt gaagcgaatt gtgttattca
     5041 tagtattggg cgtgcattac acatggcagc acgtcgtaag atgactgaaa aagaattagt
     5101 catgctgaaa caagcaaatg ctgttgcgga atcttttggc tactttttca atgaaaaagg
     5161 tgaagtcgtt tataaggtcc ctagaatcgg catagaattg caagatttgg aaaaagtccc
     5221 gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag cttatatgaa
     5281 aaatgcacca aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg agattttaaa
     5341 aggggtaacc ctttaaaata aatatttttt tgattttcat aaggaggaaa tcttaaatga
     5401 cagttaaagt aggtattaat ggttttggac gtattggacg tctagcattc cgtcgtatcc
     5461 aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt gacagatgct aaaatgttgg
     5521 cacacttgtt aaaatacgac acaactcaag gacgtttcaa cggaacagtt gaagttcatg
     5581 aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc aacccagaag
     5641 aattaccatg gggcgaacta ggcgtagata tcgttctaga atgtactggt ttcttcactt
     5701 ctaaatcagc agctgaaaaa catttaacag ctggtgctaa acgcgttgtt atctctgctc
     5761 ctggcggaaa cgatgttcca acaatcgttt ataacacaaa ccacgaaaca ttaactggta
     5821 aagaaacagt tatctcaggt gcttcatgta ctactaactg tttagctcct atggctaaaa
     5881 cattgaacga caaatttggt gttgttgaag gattaatgac aactatccac gcttacacag
     5941 gtgaccaaat gactctagac ggacctcatc ctaaaggtga cttccgccgt gcacgcgctg
     6001 ctgctgcaaa catcgttcct aactcaacag gtgctgctaa agctatcggt ttagtaattc
     6061 ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt tcctgtacca acaggttcat
     6121 taacagaatt agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc aatgcagcaa
     6181 tgaaagaagc ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt tcttcagata
     6241 tcgttggtat gacttacggt tcattatttg atgctacaca aactaaagta atgacagttg
     6301 gcgacaaaca attagttaaa actgttgctt ggtacgacaa cgaaatgtca tacactgcac
     6361 aattagtacg tactttagaa tacttcgcta acttataaga tagacaaacg tacaagtgta
     6421 aattatagac atgtacattt aaaagcgggg aagcaatcgc gcttcttccg ctttttttga
     6481 taataagttt attcaggagg aaataaaatg gctaagaaaa cagtgaaaga tatcgattta
     6541 aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga cggcgtgatc
     6601 actgacgaca ctcgtatcaa agcagcattg ccaacaatca actacgttct agaacaaggc
     6661 ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga taaagaagga
     6721 aaatctttag ctccagttgc aaaacgtcta ggcgaattat taggtaaaga agtaacattc
     6781 gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa tgacggcgac
     6841 gttgtcgtat tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga aagcaaaaat
     6901 gatacagaac taggtaaata ctgggcttca ttaggcgatg tatttgttaa cgatgctttc
     6961 ggtacagctc accgtgcaca tgcttctaac gtaggtatcg cttcaactgg tatcccaaca
     7021 gttgctggat tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt agaagaacca
     7081 aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt ctgataaaat cggcgtaatc
     7141 gaaaacttga ttcctaaagc agataaaatc ttgatcggtg gcggaatgac ttacacattc
     7201 tacgaagcaa aaggaatcaa gatcggtaac tctctagtag aagctgataa agtggaacta
     7261 gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct tgccaatcga caatgtctgt
     7321 gcaccagaat tttcaaatga tgttgaaaca caagtaatcg aaggagacat tcctgatggt
     7381 ttgatggctt tagacatcgg cccagcatca gtaaaattat ttgctgatac attacaaggt
     7441 gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa ctttgctaaa
     7501 ggtacaatcg gtgtttgtga agcaatcgct aacttagaag gcgcaacaac aatcatcggt
     7561 ggcggagact cagctgcagc agcagaacaa ttaggatttg ctgacaaatt cacacacatc
     7621 tcaacaggtg gtggcgcaag cttggaattg cttgaaggta aagaattacc aggactagca
     7681 gcaatcaacg ataaataaac gaccaagttt gctcaattct gacgagtaat cgatcaaaag
     7741 gtgaagggag ttttcatgac tccctagcca tttttcaata aattaaggag tgtgttcctc
     7801 aatgcgtaaa cctattatcg caggtaactg gaaaatgaac aaaactgcat cagaagcaaa
     7861 agaatttgct gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg attcagtcat
     7921 cggatctcct gcattattct tacaagaatt aatgacatca gctgaaggta cagaactaaa
     7981 aatctcagca caaaactgct actgggaaaa ctcaggtgcg ttcactggtg aaacttcacc
     8041 agcagctctt gctgatctag gtgttcatta tgtgatcatc ggacattcag aacgtcgtga
     8101 atatttccat gaaacagacg aagacatcaa taaaaaagca aaagctatct ttgcaaatgg
     8161 tatgacacca atcctatgct gcggcgaatc tttagaaacc tatgaagcag gcaaaactgc
     8221 tgaatggatc gaaggacaaa ttacagctgg tttagctgat ctttcagctg aacaagtgtc
     8281 aaacatggtt attgcttatg aaccaatctg ggcaatcggt actggtaaat cagctgacgc
     8341 acaaatcgct gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac tttatggaaa
     8401 agaagtatca gaagcagttc gtatccaata cggcggttct gtaaaaccag aaaatattgc
     8461 tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa gcttgcaagc
     8521 agattcattt ttgtcattgt tagacgctgt aaaataattg ttgaaaattt gcgaaaaatg
     8581 caagaaatgt ttgcactaat gagcaaaatt aactaaaatc gtatatgagg gaaatccctt
     8641 aggaaaaaac aaactcaaag gagagacaaa acatgtcaat tattactgat gtttacgctc
     8701 gcgagatctt agactcacgt ggtaacccaa caatcgaagt agaagtatac acagaaagcg
     8761 gagcttttgg ccgcggaatg gttccttcag gtgcttcaac tggtgaatac gaagcagttg
     8821 aattacgtga cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa gctgtcgaca
     8881 acgtaaacaa catcattgct gaagcaatca ttggctacga tgttcgtgac caaatggcta
     8941 tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa aggtaaatta ggtgcgaacg
     9001 ctattttagg tgtttctatc gctgttgcac gtgcagctgc tgactaccta gaagtacctt
     9061 tataccacta cctaggcgga ttcaacacaa aagtattgcc aacaccaatg atgaacatca
     9121 tcaacggcgg atcacatgct gataactcaa tcgacttcca agaattcatg atcatgcctg
     9181 ttggcgctcc aacattcaaa gaagctctac gtatgggtgc tgaagtattc cacgcattag
     9241 cttcaatctt gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt ggatttgctc
     9301 caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc gaaaaagctg
     9361 gctatgtacc tggtaaagac gttgttcttg ctatggatgc tgcttcttca gaattctacg
     9421 acaaagaaaa aggtgtttac gtactagctg actcaggcga aggcgaaaaa acaactgacg
     9481 aaatgatcaa attctacgaa gaattagttt ctaaatatcc aatcatctca atcgaagatg
     9541 gattagacga aaacgactgg gacggattca agaaattaac tgacgtatta ggcgacaaag
     9601 ttcaattagt tggtgacgac ttgttcgtaa caaacactca aaaattatct gaaggtatcg
     9661 aaaaaggaat tgctaactca atccttatca aagtgaacca aatcggtaca ttaacagaaa
     9721 ctttcgaagc tatcgaaatg gctaaagaag ctggctacac agcagttgta tctcaccgtt
     9781 ctggtgaaac agaagattca acaatctctg atatcgctgt tgcaacaaat gctggccaaa
     9841 tcaaaactgg ttctctatca cgtactgacc gtattgctaa atacaaccaa ttactacgta
     9901 tcgaagacca acttggtgaa gttgcagaat acaaaggttt gaaatctttc tacaacttaa
     9961 aaaacaaata attcttttct aatgaagata catgatcgac tattgaaaga tagtcgagta
    10021 tgatcactta gttaagtaat cgaactctct acttgctgaa atatcagcag gtagaga
//
LOCUS       DACDHJ010000081         9976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDHJ010000081 DACDHJ010000000
VERSION     DACDHJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.083"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            282..1412
                     /locus_tag="IV223_001536"
     CDS             282..1412
                     /locus_tag="IV223_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8711828.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1504..2259
                     /locus_tag="IV223_001537"
     CDS             1504..2259
                     /locus_tag="IV223_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8711829.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2317..4356
                     /locus_tag="IV223_001538"
     CDS             2317..4356
                     /locus_tag="IV223_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8711830.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4465..5616)
                     /locus_tag="IV223_001539"
     CDS             complement(4465..5616)
                     /locus_tag="IV223_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8711831.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5770..6300)
                     /locus_tag="IV223_001540"
     CDS             complement(5770..6300)
                     /locus_tag="IV223_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711832.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6399..6749)
                     /locus_tag="IV223_001541"
     CDS             complement(6399..6749)
                     /locus_tag="IV223_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8711833.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6906..8435)
                     /gene="ahpF"
                     /locus_tag="IV223_001542"
     CDS             complement(6906..8435)
                     /gene="ahpF"
                     /locus_tag="IV223_001542"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8711834.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8445..9008)
                     /gene="ahpC"
                     /locus_tag="IV223_001543"
     CDS             complement(8445..9008)
                     /gene="ahpC"
                     /locus_tag="IV223_001543"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8711835.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9152..9814)
                     /locus_tag="IV223_001544"
     CDS             complement(9152..9814)
                     /locus_tag="IV223_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8711836.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
BASE COUNT         3282 a         1876 c         1865 g         2953 t
ORIGIN      
        1 taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta
       61 aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg
      121 atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt
      181 tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt
      241 gtgaggagga gatgcggtga aaaaagatcg attggtggat tttgggtcct tatttgactt
      301 tgtccatgat cggtctttta gaagtataca gtgccagttc ttatcgttta ttgcaagccg
      361 atgaaaatac aaaaagtcta ttattgcgtc aactaatttt catatttttg agttggagcg
      421 tgatcttctt agctcgttca gtcaaactac actatttact tcaccctaaa atagcaggat
      481 acggtttagc cttatcgatt ttctttttag tattagtaag aatagggata ttcggtgtca
      541 ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat tcaattccag ccttctgaac
      601 tggcaaatct ttttttgatt ttttatttaa gctggttttt tcgtgacgga aatagtagcc
      661 caaaagatct aaaaaaacca ttcctgatta cagtaggtat aacttttctg attttatttc
      721 agccaaagat tgctggagca ttgatgatcc tttcgattgc gtgggtcata ttttgggcag
      781 cggcggttcc atttaaaaaa gggatctatc taatcgttac tttttctgca ttgctgattg
      841 gagcagcagg cggggtatta tatttaggaa ataaaggttg gcttccacag atgtttaatc
      901 atgcttatga aaggatcgcg actttaagag attcttttat cgatagtcat ggtgcgggct
      961 atcaaatgac ccattcgttt tatgcactat ataatggtgg catatttggg cggggattag
     1021 gaaatagtat cacaaaaaaa gggtatcttc cagagacaga aacagatttt attttttcga
     1081 ttattacaga agagcttggg ctaatcggtg cattgtgtgt gcttttctta ttgttctcat
     1141 tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa tcagcaagct ggattgtttc
     1201 ttttaggatt cggaactttg ctattcgttc agacgataat gaacgtggga agtatcgcgg
     1261 ggctgatgcc aatgactggt gtgccattgc catttgttag ctacggagga acaagttatc
     1321 tgatcctttc tttaggaatc ggaatcacgt tgaatatttc ttcaaagata caggccgaag
     1381 agttggcgct ctatcgacct gaaaaacaat aaagagaaac tgagaaactc tcagtttgaa
     1441 ttatcataaa gtacaatctt ttatttttca agcgagaggt tcttttagaa agaggaattc
     1501 gcaatgaaag tatctcaaaa aatcatatta tctattttgt tactgcttac attagtagtt
     1561 ggctattttt cgattgagtt tatccatgga ttttcgtctg caaaacagag ttcaactgtg
     1621 aaaaaggtag agccgaaaag tatccctacc acactaaatg tggctttgat tggttcagat
     1681 gctcgttcga aagacgaaaa cggtcgctcg gattcacttc tggttgcaca atacaaccag
     1741 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
     1801 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
     1861 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
     1921 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
     1981 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
     2041 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
     2101 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
     2161 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     2221 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     2281 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     2341 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     2401 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     2461 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     2521 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     2581 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     2641 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     2701 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     2761 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     2821 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     2881 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     2941 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     3001 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     3061 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     3121 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     3181 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     3241 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     3301 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     3361 gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc aactgttgcg
     3421 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     3481 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     3541 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     3601 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     3661 cttaaatggc aaaaagacag ttcttgggga tcttatcaag caacgcgtgt cagtagtgat
     3721 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     3781 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     3841 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     3901 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     3961 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     4021 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     4081 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     4141 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     4201 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     4261 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     4321 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     4381 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     4441 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     4501 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     4561 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     4621 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     4681 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     4741 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     4801 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     4861 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     4921 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     4981 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     5041 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     5101 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     5161 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     5221 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     5281 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     5341 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     5401 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     5461 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     5521 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     5581 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     5641 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     5701 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     5761 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     5821 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     5881 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     5941 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     6001 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     6061 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     6121 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     6181 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     6241 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     6301 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     6361 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     6421 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     6481 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     6541 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     6601 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     6661 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     6721 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     6781 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     6841 gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt tacaatcttc
     6901 atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc
     6961 attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc gaaaacgccg
     7021 gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt catttcgatc
     7081 gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac tcctgaaaca
     7141 gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc tacctgattc
     7201 gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc aagagagact
     7261 agtttatcct gtaagatttg atctgctttt aattctggta agaattctag aacatataca
     7321 tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc accaccgata
     7381 acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata agcaattcct
     7441 ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg
     7501 atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt aataaaatgt
     7561 tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg gtaactacgc
     7621 acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt accaatcata
     7681 ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac aagtcctgta
     7741 cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc accgccaatc
     7801 actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg gccagtgatt
     7861 ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc atttaaaaac
     7921 acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat cccaccttca
     7981 atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc ctgtacaacg
     8041 tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt ttgtgttatt
     8101 tttttgattt taagactcaa agattcactg attttcggtg cccgtccact aatttgtaac
     8161 atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa aacgattcca
     8221 ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc
     8281 caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag tttttgacta
     8341 ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag tgctaaatat
     8401 tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat ctttccaaca
     8461 agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg acaaacttca
     8521 cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc actagcgtca
     8581 cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg atcaataata
     8641 aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat acgagaaatc
     8701 tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc tgatgtatca
     8761 tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc aacacctaaa
     8821 gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca aacaaaagta
     8881 aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt ttcactgact
     8941 tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt tttcccaatc
     9001 aatgacatag taaaacctcc aataataatt atttatttat aattattgta atttagttgg
     9061 agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa cgaaggttaa
     9121 ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg ggataacgtt
     9181 gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag
     9241 cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt tccatgatcg
     9301 tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct aaatgggtat
     9361 tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa caagcttcat
     9421 gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact gctttttctt
     9481 gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg gaacaacatt
     9541 caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct gcagaaacaa
     9601 gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt tcttgctgac
     9661 gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt tcctccagtg
     9721 ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt ttacgaatcg
     9781 cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa tcatttctat
     9841 gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt aactagtaaa
     9901 tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa aattcacttg
     9961 aatacaaaaa ctaata
//
LOCUS       DACDHJ010000082         9972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDHJ010000082 DACDHJ010000000
VERSION     DACDHJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.084"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(487..954)
                     /locus_tag="IV223_001545"
     CDS             complement(487..954)
                     /locus_tag="IV223_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8711837.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(1029..1469)
                     /locus_tag="IV223_001546"
     CDS             complement(1029..1469)
                     /locus_tag="IV223_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8711838.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(1499..1792)
                     /locus_tag="IV223_001547"
     CDS             complement(1499..1792)
                     /locus_tag="IV223_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711839.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(1823..2017)
                     /locus_tag="IV223_001548"
     CDS             complement(1823..2017)
                     /locus_tag="IV223_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711840.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(2123..2575)
                     /locus_tag="IV223_001549"
     CDS             complement(2123..2575)
                     /locus_tag="IV223_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711841.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(2601..3062)
                     /locus_tag="IV223_001550"
     CDS             complement(2601..3062)
                     /locus_tag="IV223_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8711842.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(3263..4114)
                     /locus_tag="IV223_001551"
     CDS             complement(3263..4114)
                     /locus_tag="IV223_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8711843.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(4129..4929)
                     /locus_tag="IV223_001552"
     CDS             complement(4129..4929)
                     /locus_tag="IV223_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8711844.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(4972..5862)
                     /locus_tag="IV223_001553"
     CDS             complement(4972..5862)
                     /locus_tag="IV223_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP8711845.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(5864..6805)
                     /locus_tag="IV223_001554"
     CDS             complement(5864..6805)
                     /locus_tag="IV223_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8711846.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(7038..7373)
                     /locus_tag="IV223_001555"
     CDS             complement(7038..7373)
                     /locus_tag="IV223_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711847.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(7699..7887)
                     /locus_tag="IV223_001556"
     CDS             complement(7699..7887)
                     /locus_tag="IV223_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711848.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            7965..8189
                     /locus_tag="IV223_001557"
     CDS             7965..8189
                     /locus_tag="IV223_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8711849.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(8481..8693)
                     /locus_tag="IV223_001558"
     CDS             complement(8481..8693)
                     /locus_tag="IV223_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711850.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(8696..8908)
                     /locus_tag="IV223_001559"
     CDS             complement(8696..8908)
                     /locus_tag="IV223_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8711851.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            9183..9593
                     /locus_tag="IV223_001560"
     CDS             9183..9593
                     /locus_tag="IV223_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8711852.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            9606..>9972
                     /locus_tag="IV223_001561"
     CDS             9606..>9972
                     /locus_tag="IV223_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8711853.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDE"
BASE COUNT         2792 a         2069 c         1576 g         3535 t
ORIGIN      
        1 catggttgcc gaagcattga cctagcacac atgcttagcg tctacccttt ccgccacagt
       61 gacactataa aattattctt ggctgctact attttttatt ttgcccattt ttaaatccaa
      121 tcatatatac attaagacaa agcgcaaaaa ttgaaattat taatggaatc atttctcttc
      181 acttaccttt agttatcgtg tgaataatta aaaaaacaat agacagcaac ggatgataga
      241 taataagaac aatttagaag gagttgaaat tcacatcctt attcttaata tttccgttgc
      301 tgcctatcga agcttaatta aacgatgagg gagatttcct cccttacatt ttattttgtc
      361 gatcctgttt cctaattttt cgacactatc ataatacaac gttgaatagg taagtgattg
      421 gtataaaaaa ggtataaaat ggaaaccaaa ttggtaataa aagggtataa aaagtgtaaa
      481 aactggctac ttgaaagcaa ccagttctaa cgatgaagca aattggatga taattctgtt
      541 tgattctact ttaaccgatt cttcgctagt attattcctt tgagcagtta cataaatggg
      601 cagaccattg atataacgat catagaatat cttcttgcgc ctttcagtca catcaggctt
      661 atgcggatgc tgtatcgctg aatagcctcg aacaaacaat ttatgcagat actcaaattc
      721 ttcctgtgct tcttctttat cgatcagcat tctttctgct tcaaatatat gatcagctgt
      781 agaaggtgga accaaggaat aagatgctgt cacttttggt tctcgatgtt gccctactct
      841 acatctagct gatagatatg ctgaaagaaa cacagcgaca ttatgttttg tgcgttccat
      901 atcaacatct ttcgcgcttg gtgtctcata tttctttaca tcgaaaagta ccatcctctg
      961 attcccccaa ttatgatata atacttatgt cggaaatatt attcatagtc ggaggaatcc
     1021 gacttttttt attttgtacg tgaaatgagt tctcctgatt tatacgcctc agcaaattca
     1081 accaaagcta ctgccttcat tctctcgata gttctatcgg aatatcctaa tttttctcct
     1141 atttgataca ttgacatctt attaggaaaa caataactat aataaagtac ctggaaatgg
     1201 attctactta gccgtgttaa tgcttcgata accgaatctc gagtacttat cgcttcaatg
     1261 atttcttcgt tttgactgtc aattgtgaat ttcaaatttt tggtaacagt catcacggaa
     1321 aagtctattt tcactggtcc gaccaaacgt tctaaatttc tgtatctttt tagtaatcgt
     1381 ctagcatttt tccttgtttg cttaatatct atattctcta aaatgttcac aattgcctct
     1441 tctccttcca gttgtggaat aacgctcatt gttgtaggat agttgtaaaa aagcaaaatt
     1501 aagattttat aacttccatg tccaccaatc tcaccactgc taaattctct ttgcttttcg
     1561 ctaaccactt atcacattcc atcgtgtttt caatgcgaat gattgctgag tgattataga
     1621 cgtgttctac atatccacga aatggataga tgaatccttc tgcttcgcag cgaaccatgt
     1681 caccgacttt gaattttggt ttcttacgtg ttttaggatt ctttgtcggc atatctagca
     1741 ttaaaccgcc gatgccgtgg ccgctggagt aaaatccgtc ttttagtttc attccgcttc
     1801 ctcctcttta gctgtcaatt acttactcgc aagtcactaa aatggctact tcatcgataa
     1861 agtttccgtc ttcgtcttcc attccttgct gaatgctaat gtcggtaatc tgcttaaaaa
     1921 attcgttgtc atagccttgt gcgtttatca ctacttcgac gtctccgaaa acctctttga
     1981 gactattaat tttatttact acttccgatg cgtccatgac tacacccctt tccagttaaa
     2041 aatctctgtc gttaatagac tactgttcaa agtattcatc ttcactcatc cttctgcctc
     2101 ctctttagtt gtcattagtg gactaaatac tacctgtttg aaaaccataa tgtgcagttt
     2161 caacgccgtc ataatcactt atttcccact cagccccatc gggtatatca acgactttaa
     2221 gacttgcata tggtccactg gcctcacttc ctagtttttc gacaacagca acgaggtcct
     2281 tatcacctct gtttgaatca cttgaaaaac tggaatcatc agcaattccg tacagtcggt
     2341 caatttcgct ataaattcct gtaactttgt cgattttcgg gtcttttttg aaatagtaga
     2401 taaaatccat cgaacctagc ttttggcctg tgtatctcgt gtattcatca tctgagtcat
     2461 agctaaagtt attgatgtat gggaatactt caatattttt tcttttaaaa atttccattt
     2521 ttgcttcatg gctcaaccca aagccaccaa aacatttatt taatacaatc ttcattactt
     2581 ttcctccttt gtttcctccg ttagctgaat tttttcataa acacaagcca atgtgttttg
     2641 cttcgtttat taccaaacaa tggctgttct ccaatagcct ctaacacatc agaaagtggt
     2701 atctgttcct cgttccactt aaaaattaat gttcctgaag gtcgtaaaac tcgcatacac
     2761 tctgcaaaac cttgcctgat gtcttctggc caaagttcgt ctaattttcc atattttttg
     2821 gcaagccatg agttttcgcc gacatgtctt aagtgtggcg ggtcaaatac aaccatatcg
     2881 aacacatcat cttcaaaagg catatcgcga aagtctgcga ccacatctgg atcaacattg
     2941 atcacatgac ctgttgataa ttcttcatac tgctgacgac aatccataaa tgttacgtca
     3001 gaattgttct tatcaaacca gaacaatcta ctaccgcaac atgcatctaa tatttttggc
     3061 aattactcta cctccaataa ctctggattc tcgtagacgt tacctaaaac agtgagaaat
     3121 ctttctgtat cccgataaat aattagctta cttatcttcc gaaccattcg actctcttgt
     3181 tgagataatt cgtccatgtc acgatcacct ataatttgca acattatctg acgacagatt
     3241 cttttttctt gtttagtcaa attcatgcga tcgctccttt gactggtttt atgcgcttgt
     3301 ctgctgtttg agaaaatacc atcgtaaatc catcagagtt gacaaacatt cttgatattg
     3361 ttctcgtacc gtaagctttt ttcagttctt caccaagcaa gttcgttgtg ataatcgttg
     3421 ctaggttctg tcgtgcatcc aagaacgaat ttagcgtgtt tatgccaaac gctcgactat
     3481 ctgatgcatc ttttcctagt tctgaaccga tatcatcaat gatgactaaa tccgctgttt
     3541 tgatatctgc aatcaacgat ccctcaatcg tttttctgag ttcaggattg ttgtacgaaa
     3601 atttgatttg atccagcatt tcctgtaacc cgataaataa gattttttta tcgtagtttg
     3661 atcgcttcaa gacttcccaa gccgctgcca tcgctaggtg acttttacct gttccttgtt
     3721 ttccagttaa aacaaggtga cttggatttc ctaacaagac tgaattaaca aagcgtttgg
     3781 ttacttcaac cgcttgtctt gtttcttgat cgacaatttg ataattctgc aatgtgcaat
     3841 caaacaacgc ttgattaggc acaacagaac cacctttgaa aaaattgatt gctcttgctt
     3901 tcaggctctc gttgtaaatt cgttctgttt gtaagtcttc ctgaactcgc aatgctttat
     3961 acccacattg catacaggtt ggcttacaac gttctgagcc atcagggttc ttcgtacgcc
     4021 atccatacaa aggctgtccg cattcaggac attcgccacg ttggacaagc actttctgta
     4081 tcagcttttc catgatttcc ccaacagttt ccatgcctac gcctcctctc aaataggcaa
     4141 ctcatctgtg ctaaatttct cgtattcaag cgttttagtt tgttttgttt tataatcacg
     4201 atttgcttgc gctgccattg tgtcgtattt ttctcgcaat tttttagcag ataaaatatt
     4261 cgatgcccaa aacacattgt gctgactcca ttcaatcatt ccccgtacct ggttttcggt
     4321 ccgcttgtcg atctcgatca ttttccgaat gtcatctgcc caactttgca gattcggctt
     4381 tttgatttcc tgattctgac aaatctgttt gaataactcc tccgcgagaa tgtagtaaac
     4441 tgagtcggtg tcgtaaacac gctttttgcg tggttgcgac gatgatgttt tattattctt
     4501 ttcattctta taattctttt cattcttgtt tgtgtgcact tgttgttcac ttgttgtgcg
     4561 tttgatgttc atttgttgtt cactgctttg gtacaaagac cagtttttta ttgatataac
     4621 gctgtatttc gtagttgatt tgatgttcaa cattccgttt ttttcaaatt gttttagcca
     4681 tctccataca gaaccgctgt tcacttgatg ttcacgtttc acgccttcgt tcatctcaga
     4741 tgtgatagca tcgcgccctg tgacgaattc tccgctgttc acttgtatct cttttccatt
     4801 aaaaagtatc ttgcgattct catgactcgc tttcatcaaa cacaagttcc ataatttata
     4861 catataagga ttggtccaaa cgaatgaatt catcactttt cgatgtaatt tgacatatcc
     4921 tgtgttcatt cgtatttctc ctctagaaaa gcaagggaga aagctccctc attatttgtt
     4981 taacggcgga ttagatgcat caaataatcc agtttgtaca tcttcatttt cttcagatat
     5041 aacctctgct tcttttcttt caggaatatc ttcttcaact tctgtttcag caataatgct
     5101 gccgtcttct tgaacccttt ggactctctc atccgatgtg gtggcttctt gcatttcgat
     5161 ggacaagatt ccccatttag aaagaagatt tctcaaaaca gtttttcgtg ccattgcatt
     5221 gtaatcagat gcccacacac cacttaactt tgtcttatcg cgatctttat tgttagcaat
     5281 ccgatgagct tcgatttctt gtttggtcca atagacagtt tttttgaatc cattcagtaa
     5341 ctcgaaatag ccaacatatc caatgacttc atcagacgtt ctaccatttg gatcaaactc
     5401 aaactcttct gtcagtcggt tccagctttt tagttctcct tcgtaaactt cgatcacatt
     5461 taatgctttg tatttacctg atcgttgggc taattggata tatcctttat agccaagcat
     5521 gaattgagct ttcttttccc attttcctgt ttgcttgttt ttactattga atggaactaa
     5581 atatgcataa cctaaattct tatctagccc aagatttaat gttgcagcag ttaacgcacc
     5641 actcatgata gacatcggtt cactatctgc aagataactg tcattagata caagagtcat
     5701 aacattcgac ataaaagcat tagcattgtc atgaagcacc tcttcgaact tctttctcat
     5761 tgttggtgta ttcatcaaag ctttaagtcc taattgacct ggtgcaactt gtttctgtgg
     5821 ctttgctgcc aattgatttt ttaacgattc atttgttgcc atattatttg atctcccttt
     5881 cggttagcct tcttgattca gtaacgttat aaatctcttc atcatttgcg acatctggat
     5941 atttctctgc tagtttcttc gagttcatac gtctcgtacg gacaaatttc caactgatga
     6001 tgtttttttg agtgataccg atactggctt cacgtttacc tagctcgctg ataatctcgt
     6061 tgtctacttg acggatagct gattcaattt ctttcttagt ccgtttgagt tcgtttttct
     6121 gttcaactag ttcatcgaaa tgaattggta acgtcgtttg aacgtcttca acatctgcat
     6181 acttctcttt caagaaatca gctgtcgctt gactaccatc aataattggc tcgatacctc
     6241 caagaacgtt tgtttcccaa aactctacta attgttcagt gattgtatcg atcagctctt
     6301 gatctcgttc aatccgcttc cagataaatt tttgaccacc gattaagaca gcgatgtaac
     6361 aatagtcttt gtttagaacg ttcatgtaat gttgaacttg acagagatag ctaagcggta
     6421 cctcttcgcc ttcccattct ttaccgagaa attgattggc tgttttgcat tccagaatgg
     6481 cattttctcc cactacttcc cgatcaatat ttgctcttaa aaatggatgt aatggatgtt
     6541 caaatacttg gtttcttctg cgtacttttt tgcctgtacg ttcctgaaac tctttagcaa
     6601 ccacttcttc taaaacatta ccccaataag cgggctcact tcctgattct tcaagtacta
     6661 cttgtcctgt tttttctagc catagttgat aaggagattt ccatttattc aatcctaaaa
     6721 ttgttccaac atccgaacct ccgatgcctt tcttacggtc ttcaagccat tcttgatggc
     6781 tcatttctaa ggtagattta ctcatcttca tcctcctcat caattggtgc ttcatatggt
     6841 ggtttagcat aatcaggatc agttaaatag ttgtcaagat ttgctaactc gttcatgttt
     6901 aacctctttt ctgcttgcta acacgcaaag cacaagcatg gtaattgcga ataacaagcc
     6961 aaagattaca taacttttcg ctacaatcac taacgtgaaa aataaaataa tgagtagatc
     7021 aattgttttt tcattcatca ttgaaattcc cccttgagaa tacgagtcat cacatcagct
     7081 aaatcttcct tgttattaat aacaaaagta tgatttgaag gttgattttc ttcttcattt
     7141 ttcgcttgtt cgtaagcatc cacagcaatt ctacgcataa tatgtgctga caatgggaga
     7201 ttttcatcta aatgttcttc ttgcatagca agacaaagta aaatttctgt tgtctcacca
     7261 taaacgagac ttttaagttc cccttctttt tgggctgtac aaataataga aacaccttgc
     7321 ttttggcatt cacgtttcaa ttcttcgata agattttcaa ttttcttatt catgtgatat
     7381 aattctccta gttttataaa tttgtatgtg actctttgct ggctggcaga gtcacttttt
     7441 tatttgttgc catgcttttt gcttttcgat atgttgcttg cttaaaataa tagggcggct
     7501 atttccccac cgattatcag caatcacttt accgattttt agcgcttctt ctcgtgccat
     7561 agttgctcct ttcttttgaa tcaagcagat tgattaaaac catcaatgct gcgaacaagc
     7621 ttcccccgat aatactttgg tgcgctacaa tcactaataa ccccaaaatg aatcctataa
     7681 aaagtgtgtc tgtcttcttc ataatctaat ctccctattt tttatttcta gcattctcaa
     7741 atcctcaagt tcagaagcaa ttagttcagc ttgcctatct gatagctcat cggcttttct
     7801 aagcgctgca cggtcatctt gtaattgttt cctgcgttgt ttaatcaaac cgagaatttg
     7861 atgttcttgt tgcaaggtat atgccatatc acttctcctt tcgtttattg aatttctcca
     7921 tttctatttg ataaaatatt tctatagaaa tggaggtgaa aactatggga aaaaatcaac
     7981 atgttgtacc taattccaat ggtggttgga atgtcaaagg tgctggaaat tctaaagcga
     8041 ctgcacatac taatacaaaa tcagaagcag taaaaatcgc tagagagatt tctaaaaatc
     8101 aaggatctga gctatttatt catggcagag atggaaaaat tcaaagccgt gatagtcacg
     8161 gaaacgatcc acacccacca aaaggttaat catatttagg agttaatcga atacgataac
     8221 cgtcagcagg agttgcatcg gtcaatgtaa cttctgctac ttttttccct tcttctgttt
     8281 caataattaa tcttgtgtaa ttgttctcat tcaaaatccc tatttttgat tcttccttca
     8341 tgtcatccct ccttcgtctc tcgccgtcac cacctccaag aagaggagtt gccaaaatga
     8401 aaaaacgtgg actttggcaa acttgaaagg agagcagcaa ctcctctttt cagaagtggt
     8461 gaatttagat tttttagcta ttaacaaaca gctctaactc taaatcgatt tttagaacgc
     8521 catgttctaa actgttcata taaatcgaca tcaattgtta tttctttgga agtaactgcg
     8581 ttatacccct cactaaaacg tgatttttta aattccttca ttcttctttg ataagtactt
     8641 tcagaatact tatatttctc tttaaactcc gctttgctca aacgttgtac catttttacg
     8701 cctcctgttc tgatggctga tcattagaat ctacttctaa cttgatatcc aaaagttctt
     8761 cgatttgttt taaacgttcc ttaggttttc ttttttcgat gaagatatct cgtaaataag
     8821 taattgatat acccattcgt cgagctaact ctctttgact ccaacctttc tttactaaag
     8881 caattaatac ttgctgtttt aaagtcatgc ttcatacccc cttatcgaat attttctaat
     8941 atttttgtct aataagtttg acaaaaactg caaatatgct attatgtaag catacaaaat
     9001 acagcgcaca aaaagcgagc ttactgatgt ctggggagac tattagttta ttgcttttag
     9061 tctttgtatg ccgtaaatat cttgcttaca aaactagtat aactgcaaat tctctatttg
     9121 taaatagata atttgcagaa aaatactttt tttgtcggta ttttgaaagg aatgcatatc
     9181 ctatgctatt agaccgtata aaaatcttat taaaagaacg ttcaatgacg ttggcagaac
     9241 ttgaaagaaa gttaaatttc ggaaatggaa gcatgtctag atggaataaa agtactccat
     9301 ctggagataa aatacaaaaa gttgcagatt actttaatgt atcaactgat tatcttttag
     9361 gacgtactga taaacgccat tattatgatt taacagaaaa agataaacaa gatattgctt
     9421 tacaagctga aaaattactt gaaggcttga attcagatgc tgaaacaaat tattatggtg
     9481 aacctttaga cgatgaagat aaggaaaaat tatacgatgc cattcaactt gcacttgcct
     9541 taaccaaagt taaagcaaag aaaaaattca ctcctaaaaa atatcgtcaa taggtgggct
     9601 ttggtatgca tttaaacaaa tactggatat tagaagaaat agaaaaatta tttgtagagt
     9661 ttggatctga tgcgattaat ccatataaaa taatcaaata ttctgatata gatttaattg
     9721 aaactgattt agggaataaa acattaggac aaacgataca caataaaaga tgttatgtga
     9781 tattaattaa tcaaaaccta tcaccttcta tgaaaagatt tgtattatgg catgaaatag
     9841 cccacgttag actccataaa ggtatttgca cagcagcttt tagagcaaac aaccttagtt
     9901 gtatgattta tggtatagaa gctgaagcta atatgtttgc aattgaaatg ttaactagaa
     9961 caatagatga ac
//
LOCUS       DACDHJ010000083         9939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDHJ010000083 DACDHJ010000000
VERSION     DACDHJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.085"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            194..1150
                     /locus_tag="IV223_001562"
     CDS             194..1150
                     /locus_tag="IV223_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8711854.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(1455..2411)
                     /locus_tag="IV223_001563"
     CDS             complement(1455..2411)
                     /locus_tag="IV223_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8711855.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            2515..3117
                     /locus_tag="IV223_001564"
     CDS             2515..3117
                     /locus_tag="IV223_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8711856.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(3205..4704)
                     /locus_tag="IV223_001565"
     CDS             complement(3205..4704)
                     /locus_tag="IV223_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8711857.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(4720..5244)
                     /locus_tag="IV223_001566"
     CDS             complement(4720..5244)
                     /locus_tag="IV223_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711858.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5542..6291
                     /locus_tag="IV223_001567"
     CDS             5542..6291
                     /locus_tag="IV223_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8711859.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            6282..7067
                     /locus_tag="IV223_001568"
     CDS             6282..7067
                     /locus_tag="IV223_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8711860.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            7036..7752
                     /locus_tag="IV223_001569"
     CDS             7036..7752
                     /locus_tag="IV223_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8711861.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7854..8873)
                     /locus_tag="IV223_001570"
     CDS             complement(7854..8873)
                     /locus_tag="IV223_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8711862.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(8892..9935)
                     /locus_tag="IV223_001571"
     CDS             complement(8892..9935)
                     /locus_tag="IV223_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8711863.1"
                     /translation="MFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILSK
                     VVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQT
                     TLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAK
                     NIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPM
                     DYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTAT
                     NILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWI
                     VVLQMIHGIGLFS"
BASE COUNT         3004 a         2018 c         1817 g         3100 t
ORIGIN      
        1 tcaatatata cttgatgcac gtttatcccc tccttttaac tattctactt aagttttgat
       61 caaaaagcac ctcttaaatc gctgctttca ttttttttaa tgtttcaagt cctctcctct
      121 ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa
      181 ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca
      241 tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac
      301 ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta
      361 tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca
      421 gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc
      481 catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt
      541 gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat
      601 aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa
      661 agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga
      721 tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt
      781 tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg
      841 taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt
      901 cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg
      961 tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga
     1021 agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg
     1081 ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa
     1141 aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc
     1201 aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct
     1261 tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag
     1321 caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg
     1381 acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc
     1441 tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata
     1501 aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga
     1561 ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt
     1621 tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata
     1681 tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc
     1741 acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc
     1801 cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa
     1861 tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt
     1921 ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta
     1981 cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc
     2041 gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa
     2101 accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg
     2161 aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct
     2221 gttgccgtag ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc
     2281 ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat
     2341 aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt
     2401 cctatacgca tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag
     2461 ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa
     2521 tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt
     2581 atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt
     2641 ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc
     2701 aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat
     2761 gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca
     2821 gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg
     2881 ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt
     2941 atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag
     3001 aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca
     3061 aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt
     3121 tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt
     3181 gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt
     3241 tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat
     3301 ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt
     3361 tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc
     3421 gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg
     3481 tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc
     3541 gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc
     3601 ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg
     3661 ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc
     3721 gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt
     3781 gatttctttt tcttccagtt cgatttgttt ttggcgttct acgattttaa tctgcatttc
     3841 ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt
     3901 tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa
     3961 tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg
     4021 agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt
     4081 gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc
     4141 tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc
     4201 ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc
     4261 aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct
     4321 tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt
     4381 gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt
     4441 ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa
     4501 gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac
     4561 atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg
     4621 gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc
     4681 gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc
     4741 ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg
     4801 ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac
     4861 acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac
     4921 ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat
     4981 caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa
     5041 taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa
     5101 agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc
     5161 aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt
     5221 ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc
     5281 aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg
     5341 ttgtgtttat cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa
     5401 attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac
     5461 acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg
     5521 atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt
     5581 gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca
     5641 catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt
     5701 gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct
     5761 ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa
     5821 gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt
     5881 attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga
     5941 caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt
     6001 tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa
     6061 cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct
     6121 tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag
     6181 cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta
     6241 aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag
     6301 aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa
     6361 ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat
     6421 atataaactc catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg
     6481 gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa
     6541 attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt
     6601 ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg
     6661 attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag
     6721 ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg
     6781 tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg
     6841 gcggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga
     6901 agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg
     6961 gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa
     7021 aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca
     7081 gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga
     7141 aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat
     7201 gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat
     7261 gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc
     7321 cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg
     7381 atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa
     7441 ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat
     7501 ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa
     7561 gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc
     7621 aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg
     7681 gaaatcggcc agccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca
     7741 gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca
     7801 actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt
     7861 aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca
     7921 cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct
     7981 acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat agcagggtct
     8041 ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca
     8101 tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct
     8161 tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat
     8221 ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata
     8281 tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct
     8341 gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc
     8401 gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta
     8461 aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta
     8521 tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac
     8581 gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg
     8641 actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg
     8701 gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg
     8761 atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat
     8821 ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct catactttct
     8881 tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat
     8941 acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt
     9001 gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc
     9061 atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa
     9121 actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac
     9181 catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt
     9241 gctagccacg ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac
     9301 ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta
     9361 actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg
     9421 ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt caagccactc
     9481 atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa
     9541 tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta
     9601 gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa
     9661 aaaggttttg aaactagaat aagaataata taaataacta atccccaaat caagacattg
     9721 attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct
     9781 ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc
     9841 cccttttttc tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg
     9901 acaatcgaca tatctgtcaa agtcgatcga aaaatgtca
//
LOCUS       DACDHJ010000084         9889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDHJ010000084 DACDHJ010000000
VERSION     DACDHJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.086"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(114..2234)
                     /locus_tag="IV223_001572"
     CDS             complement(114..2234)
                     /locus_tag="IV223_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8711864.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2464..3642
                     /locus_tag="IV223_001573"
     CDS             2464..3642
                     /locus_tag="IV223_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8711865.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3658..4416
                     /gene="aroD"
                     /locus_tag="IV223_001574"
     CDS             3658..4416
                     /gene="aroD"
                     /locus_tag="IV223_001574"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8711866.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4439..4924
                     /gene="ybaK"
                     /locus_tag="IV223_001575"
     CDS             4439..4924
                     /gene="ybaK"
                     /locus_tag="IV223_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8711867.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4995..6248)
                     /locus_tag="IV223_001576"
     CDS             complement(4995..6248)
                     /locus_tag="IV223_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8711868.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6365..7714
                     /gene="gdhA"
                     /locus_tag="IV223_001577"
     CDS             6365..7714
                     /gene="gdhA"
                     /locus_tag="IV223_001577"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8711869.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7826..9172
                     /locus_tag="IV223_001578"
     CDS             7826..9172
                     /locus_tag="IV223_001578"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8711870.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9480..9866)
                     /locus_tag="IV223_001579"
     CDS             complement(9480..9866)
                     /locus_tag="IV223_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8711871.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3152 a         1797 c         2028 g         2912 t
ORIGIN      
        1 tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac tgtttattcg
       61 aaaagaggag cagcgacaat aattttgtcg ctgctccttt taaatttatt ttatcattcg
      121 gaagaacttg tggtcgttcc gtgaagttct tggtacgttt gcgttttata aagatcaaca
      181 gttgattgtc cgttgtgttc actaaagaca ctagttgact tgtcaccaag agatttttca
      241 gcttctttta atttctctaa gccattcttg tagtcataat cactggtatc aattgggtct
      301 aatccactat tcgtatagaa ccgaagaaga tcaccattat tgatttggtc agaaacagct
      361 aactgtttat caactttttc tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt
      421 tcagtaatag cttcaccagt ttcgttacta tagatcgtac caccgtagta agtatatttt
      481 ggtgtaacaa aatcaccatc acggaaagaa acaagctgtt catgatcttt cgagaataga
      541 tcttgtccta actgaatata attttttgta tctacaccta gtaagtgaag taatgtaggc
      601 aaggcatcaa cttgtccgcc gtaagtgtga ttgattccgc cctgtgtttg tccagggata
      661 tggatcatat aagggacacg ctgcatttgt gcattgtcgt agtttgtcca agtagaactt
      721 gtttttccaa ctaattcagc tagattttta tttcttgaat tcgagacacc gtaatggtca
      781 ccgtaaagga cgatgactga attatcatac aagccgcttt ctttcaaata attgaagaat
      841 tcttgtacgg cggtatctaa ataattggct gtcgcaaagt aaccgttgat cgtttcatca
      901 tttgtattgg ctaacgggaa tcctgcttcg tcattggtga attttgaata aggataatgg
      961 ttagatacag caatgaattt tgaataaaat ggttgttgca gacgttccaa gtattgtacg
     1021 gattgttgga aaaatggttt atcatgcaac ccgtattgga acgaattgtc atcattaaca
     1081 tcataataag aagaatcaaa gaaataatca tatcctaaat tcttgtatgt ctcgtttcga
     1141 ttccagaatg taccagcgtt cccatggaat accgcactaa tgtaaccttg tgtttgactt
     1201 aaaatatcag gtgcagcttg gaaagtattt tttccgccta attgtgtgaa caaggagcct
     1261 tgattcaagc caaacaatga attttccatc agcgtttccg catcgcttgt ttttcctgct
     1321 tttacttgat ggaaaaagtt gtcaaaactg aacgtgctgt tgctgtggta aagactattg
     1381 atgaaaggtg taacttcatg ctggagacca ttctcatctg tcaatttata atcgatcagg
     1441 aattgctgga agctttctaa atggatataa atcacatttt ttcctctagc gagcccgaac
     1501 aaatcgtcat ttggtgctgc ataatgttct tttacataat tttctacttc tttcatatca
     1561 ttgggacttg cttctgcccg cacttggttt gtctggtaag tttgaacacc gtcataaacc
     1621 atgaaagcat tcaagccaag atatttgacc aaataatcac gagagaatgt tcgagtcagc
     1681 aattctggac gatcagcttc cgccataaaa agatttcctg agaaaatcat aactgctaat
     1741 gtagagatcg caaaagccat tcttgctcgg atcgggcgag ggtccaactt gatttttttt
     1801 gcaatcagca gaacagtcaa gataataaaa tcgatccaat agatcacatc atatgggcga
     1861 aacagtcgaa tggcactttc tcccagacca ctcgctactt tcccagcacc taaaatcgtg
     1921 ttgatcgtaa tgaagtccgt gaattctcga taataagtga cattcgaaaa aacaagcaat
     1981 gtcaaaagaa aataaatgac catcatcgtg atataagcaa ctctagggcg tctgatataa
     2041 agagcaattc caagtaataa caatgtagtt gcaatagggt tgataaacaa gataaagtat
     2101 tgaatgccac tttcgattcc gagatggaaa tcaactaaat aggcaaaaat cgttttcaac
     2161 caaagtaaaa cgactagtaa agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt
     2221 tttatacatt tcaagataat cttcctttct gcacttctta atcacagaag tgtcctgaaa
     2281 ccatgttaca cagctcactt attctcattt attttactag tatgacaata tagtgtcaat
     2341 tgaaaccaga agtttatgct gtttcattaa ggttttcttt aagcttcatg aaaaaatgag
     2401 cagtaagaca agtcctatgt tttttgttat acttgggaag agaaagctaa agcaggtgaa
     2461 ggaatgaaag taaaagtaaa aaaaagagca agcaatcgat tgaaaaaagg atatccactg
     2521 atccaaaaag aagaccttgc tcatccccaa aaagaaacca cgcaatgggt ggaattagtt
     2581 gattcgcaaa accaatttat cgcaaaagga tatctaggaa agcagaataa gggaatcggt
     2641 tgggtattga gccagcagaa catcccattt gatcaatctt tttttgaaga attttttgtg
     2701 aagtcaaaag aaaaaagaac tttctttttt tcagatgatt ctactaccgc ttttcgtata
     2761 ttcaatggag aaggagacgg gatcggcggt ctgattgttg attattatgc aggttatgct
     2821 gttttttctt ggtataacga aacgttatat gcacatcgtg aattattgat tcaagctttt
     2881 cgtgaaatct acccggaaat cataggaggc tatgagaaaa ttcgatttga atcagtaaat
     2941 ctgcctgaga gccagttcat ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa
     3001 aacggagtaa aatttgccgt ttatttaaat gaaggattaa tgactgggat ctttttagac
     3061 caaaaagaag tacggggatc attagcagaa ggtttcgctt ctgggaaaac agttttgaac
     3121 atgtttagtt ataccggcgc cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca
     3181 acaagtgtcg atctagcaaa acgcagttta aaaaaaacac aggaacagtt tgaagtgaat
     3241 ggtttggatg tcgatcagca aaaaatcatt gttatggacg tattcgagta tttcaaatac
     3301 gccaaaagaa aacaattttt atatgacatg attgtattgg atccaccaag ttttgccaga
     3361 aataagaaaa aagtctttcg cgtagcaaaa gattatgggg aactggtcaa ggatagtctg
     3421 gatattttag cagaagacgg gatacttatt gcgtctacaa atgctgcgaa tgtctcgata
     3481 gaacaattcc aaacaatgat tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa
     3541 cttgctttat atcgtctgcc aaaagatttc cagacgacgg ataccttttt agaaggcaat
     3601 tatctgaaag tgtttgttta tcaaattaaa aataaccatt agaaggagcg atgcagaatg
     3661 gttcgggtaa agggattgaa tataggaagc ggtagaccaa agatttgtgc gcctcttatc
     3721 ggggccgtcc gagaggaagt attacgtgaa gcagttcttg cacgtgaagc aggggccgat
     3781 ttagtcgaat ggcgagtcga tcattatcgt gaagtttttt cttttgatga agtagcagaa
     3841 acattggttc ttatccatga gatgcttgat ggactgccgt tattatttac gtttcgtacc
     3901 ttagctgaag gcggagaaca agacatatcg atccgtcagt atcgcgacct ttatgaacat
     3961 gtgatacaca caggattagt agatatggtc gatatcgagt tgttcaaagt agaaagtcta
     4021 ggaaaaagac tgatagagga aatcaagtct ttaaacattc ctttaattat cagtagtcat
     4081 gattttaaag aaacaccagc agaccctgtc ttgctttacc gattgaatgt tatggaacat
     4141 tttggagccg atatcggaaa actggcagta actcccaata atgaacgaga tgttttgcgg
     4201 ttgatggagt tgactagacg ggcaaatgct tttgtctcca tgcctattat tacgatgtcc
     4261 atgggaaatc ttggtaaaat ttctcgtctg gttggcggaa cgacgggttc cgttatgaca
     4321 tttggttcct taagcagcga ttctacttct gctccaggac aattaccggt caaagcattg
     4381 aagcaaatta ttcaattatt aaatgaaccg ttgtagaaca agtttgaggt gatatttttt
     4441 ggcaaagaaa aaaatagtaa aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc
     4501 ctatacagag cacgaatatg agtgggacga aagccaccta agtgcgtcat cggtcgctga
     4561 acagcttcca gaaagccaat ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac
     4621 ggggcctctt gttgctgtga taccgggaga agccgaattg aatctaaaaa aattagcaaa
     4681 agtttcaggg aataaaaaag tagaaatgct ccatttaaaa gatttggaag caacgaccgg
     4741 atacatacgt ggtggctgct cgcctatagg tatgaaaaaa ctatttccta cttatttaga
     4801 tcaaattgca gaaagctatg aacaaatcat tgtctcggct ggcagaagag ggctacagat
     4861 ggagttagca cctcaagata tctgtgcatt aacgagcgga caatttgcag atataaaaca
     4921 ataaatcata cgtataaaag aaagaaggag gccgggatgt tgtttgtcca gtctccttct
     4981 ttctttttta tacattatgc tctttgcccg gcattcattc ctgctacatg ccctgtagcg
     5041 aaagccgctg tgatattaaa gccgccagta taaccattga catccagtaa ttctcccgca
     5101 aaatacagac catcaatgat tttactctcc aatgttttag gataaatttc ctttagagaa
     5161 acccctccgc cagttacaaa tgatttctca ataggaaaag ttttgttgat tgatagctta
     5221 aactctttgc atagttgaac aaaatcttga atttgttttt gagaggcttg ttgtcctgtc
     5281 agatgatcca tttcaagccg ctcaagaaag aaaactaaca atcgctcagg caatatacca
     5341 tgccaagcat tgaccaagtt ctttttggtt gctttacttt tttctgtcaa tatatgaata
     5401 agctcttgtt tggtttgtgt agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt
     5461 ttctctaatt ctttattgac aaaacttgaa caacgcagtg cagccggacc agaaataccg
     5521 aaatgggtga aaagtaaatc catcgtatgt tcagtcaata cacgaccttt tttatttaaa
     5581 acgcgtaaag tgatgtcttg taaagaaagc ccttgaagtg ttttctcctg aatatacggt
     5641 tcatccgata tcaaggggga ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc
     5701 ttcacgatct tgtacccatc tccagtagaa ccagtagatg gataagtccg tccaccagtt
     5761 gttaagataa tggcaggagc atggaactct tcgaattccg tgcgtatacc gtaaatttta
     5821 tgttctttgt ggatcaattt ctctactcga gtagagaata gaagcgtcac atctagttct
     5881 ttcagacgat tgagcaatgc ttcgataatc gtttttgatt tatttgttac aggaaacatc
     5941 cgtccatgat cctcttcttt taaatgtacg ccttgtgatt caaagaagtt catgatatcg
     6001 aaattattcc attgcgcaaa cgtactatat aaaaactttc catttccagg aatatgtgca
     6061 atcaaatcat caacaggacg attgttcgta acattgcacc gaccaccacc tgtcatcagt
     6121 agtttttttc ctgctttttt gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct
     6181 gcactgatcg ctgccatcat cccgcttgtt ccagcgccga ctacgatcac atcatacagt
     6241 ttttccattc cctcacctca aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa
     6301 atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa aggagcgtgg
     6361 aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac atcaaaagga
     6421 tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg ttatgtcttt
     6481 tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa cggaaccaga
     6541 aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc gagtaaatag
     6601 agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat tgcgcttcca
     6661 tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt tcaagaatag
     6721 tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa
     6781 atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt caaaacatat
     6841 cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag aaatcggcta
     6901 tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga caggtaaacc
     6961 tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat tagtgtatta
     7021 tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag tttttgtttc
     7081 cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag gcgggaaagt
     7141 tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag atgtgaaact
     7201 attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa accaacgtcc
     7261 ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag cggatatcgc
     7321 tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct tagtagaaaa
     7381 tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt
     7441 tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg gaggagtagc
     7501 agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa gagagcaagt
     7561 ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc aaacagcagc
     7621 cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat ttgaaaaagt
     7681 tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt ggaaatattg
     7741 tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag
     7801 agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt caaaagtagc
     7861 accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag cagcccacaa
     7921 cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg atttaccaac
     7981 gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa tccaatctga
     8041 ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat
     8101 cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa aagcaccaca
     8161 aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga tcgaagtaat
     8221 cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta ctgaaccagc
     8281 gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag aagaagcaaa
     8341 caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag aagcagatgc
     8401 agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct ctgtattaac
     8461 tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat tgatgcaagg
     8521 tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg aagcttacca
     8581 atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa
     8641 ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg gtgaatctga
     8701 aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg atttgcactc
     8761 tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg tgaaggttga
     8821 aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg gtctaggcta
     8881 cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc
     8941 tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg atgcttatac
     9001 attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt acttgaatgg
     9061 aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt tcgcacttct
     9121 aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt aagataaggg
     9181 tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg
     9241 tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg ctgtgacaag
     9301 acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct ctttagtttg
     9361 ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt ttttgttttc
     9421 tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat attcttagct
     9481 taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt aattactttt
     9541 ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc ggggtaagca
     9601 gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct tttttcaccg
     9661 ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc cccattatct
     9721 gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata atctttacct
     9781 acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat caatatgata
     9841 acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttt
//
LOCUS       DACDHJ010000085         9886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDHJ010000085 DACDHJ010000000
VERSION     DACDHJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.087"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1491)
                     /locus_tag="IV223_001580"
     CDS             complement(<1..1491)
                     /locus_tag="IV223_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8711872.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFV"
     gene            complement(1813..3165)
                     /locus_tag="IV223_001581"
     CDS             complement(1813..3165)
                     /locus_tag="IV223_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8711873.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3318..4394)
                     /locus_tag="IV223_001582"
     CDS             complement(3318..4394)
                     /locus_tag="IV223_001582"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8711874.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4590..6335
                     /locus_tag="IV223_001583"
     CDS             4590..6335
                     /locus_tag="IV223_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8711875.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6615..7052)
                     /locus_tag="IV223_001584"
     CDS             complement(6615..7052)
                     /locus_tag="IV223_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8711876.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7036..8409)
                     /locus_tag="IV223_001585"
     CDS             complement(7036..8409)
                     /locus_tag="IV223_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711877.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8731..9219)
                     /locus_tag="IV223_001586"
     CDS             complement(8731..9219)
                     /locus_tag="IV223_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8711878.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9241..9843)
                     /locus_tag="IV223_001587"
     CDS             complement(9241..9843)
                     /locus_tag="IV223_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8711879.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2888 a         2114 c         1788 g         3096 t
ORIGIN      
        1 tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc tgttattatt
       61 tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac gacgattacg
      121 gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca ttggcaatgg
      181 gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg tcgtttttag
      241 caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg ctgtttgcag
      301 gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa
      361 ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt
      421 caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag tcacagacat
      481 accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt ctggatcttg
      541 agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac gtgcagcaac
      601 gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata cgctcattcg
      661 tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg cttctaatcc
      721 gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta attcaggtgt
      781 ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg cgcggacata
      841 gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat gatgtggatt
      901 tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg agaaaagtaa
      961 tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca agaagcccat
     1021 attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa gtttcaatgt
     1081 gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat gtggacgatc
     1141 tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt gtacacgtac
     1201 ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta attcacgtgt
     1261 tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt ctaacattgg
     1321 taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc
     1381 agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg
     1441 ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca aatgaatcct
     1501 cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt tgttcggtca
     1561 aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct tagcccctgt
     1621 ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt
     1681 tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg caaaaccagt
     1741 ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg ttttctgcaa
     1801 gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata agcccatgct
     1861 gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga tagcatcaat
     1921 caatttattc ttttcttttt caaaatagaa cacaggtagt tgggggtatt tttggttcaa
     1981 ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga atttctcagg
     2041 atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta attccagcat
     2101 atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag tatctaatac
     2161 cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg caccatttcc
     2221 tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc cttggcgtat
     2281 ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag cattcgttac
     2341 attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa aatcggtttg
     2401 tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac cgaatgttgt
     2461 gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac
     2521 gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag cgattccttc
     2581 tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg cagcttttgg
     2641 ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca tttccaaaac
     2701 gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt
     2761 gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg cggttagatc
     2821 tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc ctactttttc
     2881 caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag gaataactgg
     2941 aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg ctccgtttgt
     3001 gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa cgaataaact
     3061 tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca gtgcagagca
     3121 ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga tctctcctaa
     3181 aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc tcacttccaa
     3241 gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa tcactccagc
     3301 ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg atggtatcga
     3361 ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc catattttcc
     3421 cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa ttcattcagg
     3481 aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct tccacctaac
     3541 atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc tggcacttcg
     3601 gctggaatct gcatttcgat tttattattg atatattttg cttcataatc atagaatgct
     3661 acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc taatacagca
     3721 acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc atactgatag
     3781 gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat gccgacacta
     3841 gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc acattgatca
     3901 aatacttttt taggattttc tttccattga ttcttaagta ctggtacata aggaacttgc
     3961 ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc acatgcactg
     4021 gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt
     4081 ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc ttctttgatt
     4141 tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact tgggtcccat
     4201 gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc tttgacccat
     4261 tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata aatggcattt
     4321 aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc gccatatagt
     4381 aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat ttactctcat
     4441 attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga gccagcttct
     4501 aaaagaacga acgtcattta ttttttgtca gctagattat ttgtcccgtt ttttataaaa
     4561 agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc agccgcttgg
     4621 cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta cagcaaaaaa
     4681 atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc aagataggac
     4741 agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca ttcaaaacat
     4801 tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc gaaacagaac
     4861 tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt gcctctgctt
     4921 ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc gttaagatcc
     4981 aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa cgagcgatcc
     5041 ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta ctccaagaag
     5101 taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat
     5161 tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat gacgcttttt
     5221 gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat catttaatga
     5281 acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag ctgaaacaag
     5341 aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat ggttttttcc
     5401 atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa gaacagacac
     5461 aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt gcttttcgag
     5521 cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc gattttggta
     5581 tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt ttagcgctgt
     5641 atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag caagaaggct
     5701 cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag tattatgatt
     5761 ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc
     5821 aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt gggacattag
     5881 aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct tcgccttttg
     5941 ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa agcggattag
     6001 acttattgga agggatgaag gctgcgccta agatccctca acagctccac catctattag
     6061 aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa gacaaattat
     6121 tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta gctgcattga
     6181 tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag gtcgtttcct
     6241 tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg gcaatcgatg
     6301 cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca
     6361 gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg tgaaaattcg
     6421 gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt
     6481 tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta tcctgtttcc
     6541 ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct actatcttct
     6601 gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga catatgatct
     6661 ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa ctttccacta
     6721 tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt tccagagtga
     6781 tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc ttgcgtgatc
     6841 aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc agctccgcgg
     6901 tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg gtccacactg
     6961 gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc ttcttttact
     7021 ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc ataaatgata
     7081 aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca agataagcag
     7141 ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata gatggattgg
     7201 ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact ggtggatcac
     7261 actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg gaatagctgt
     7321 atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc agaaactaag
     7381 cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag aagaactcag
     7441 tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc atggtccgac
     7501 tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg aaagtttttc
     7561 taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat tccctagcat
     7621 tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct tcttctggtg
     7681 tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag caagatcaaa
     7741 aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc caaagaatat
     7801 caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt tgaaatccag
     7861 ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat
     7921 agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat acaggagaaa
     7981 tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac cttgcgtctt
     8041 ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca gccctagcaa
     8101 aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa gcaatgaacc
     8161 agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac caaatcgtag
     8221 actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa atggcaaaat
     8281 cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa taaaaataac
     8341 cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt
     8401 ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt cgattttagc
     8461 attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa atacagaaag
     8521 aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc
     8581 tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt
     8641 atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg
     8701 cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc
     8761 gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc
     8821 atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct
     8881 ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg
     8941 accacgtact ttatttagca aatataattt acttaaataa atttttccat ttatctcttc
     9001 ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc
     9061 tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg
     9121 tttttgacca atgatcggca caaaaacttc cggccatact tcttgtatga ctgccatcag
     9181 ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca
     9241 ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac
     9301 cgaccacggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa
     9361 accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag
     9421 tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt
     9481 tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat
     9541 tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat
     9601 cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac
     9661 ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa
     9721 agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa
     9781 aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga
     9841 catctatatt ccccacatct tttttcattt attaaaactg cctcct
//
LOCUS       DACDHJ010000086         9865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDHJ010000086 DACDHJ010000000
VERSION     DACDHJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.088"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(328..552)
                     /locus_tag="IV223_001588"
     CDS             complement(328..552)
                     /locus_tag="IV223_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711880.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            752..3520
                     /locus_tag="IV223_001589"
     CDS             752..3520
                     /locus_tag="IV223_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8711881.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3602..4021
                     /locus_tag="IV223_001590"
     CDS             3602..4021
                     /locus_tag="IV223_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8711882.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4033..4503
                     /locus_tag="IV223_001591"
     CDS             4033..4503
                     /locus_tag="IV223_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8711883.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4520..5299
                     /locus_tag="IV223_001592"
     CDS             4520..5299
                     /locus_tag="IV223_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8711884.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5286..6107
                     /locus_tag="IV223_001593"
     CDS             5286..6107
                     /locus_tag="IV223_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8711885.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6124..7173
                     /locus_tag="IV223_001594"
     CDS             6124..7173
                     /locus_tag="IV223_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8711886.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7186..8190
                     /locus_tag="IV223_001595"
     CDS             7186..8190
                     /locus_tag="IV223_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8711887.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8823..9842
                     /locus_tag="IV223_001596"
     CDS             8823..9842
                     /locus_tag="IV223_001596"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8711888.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3304 a         1641 c         2062 g         2858 t
ORIGIN      
        1 ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa
       61 gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc
      121 aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca
      181 ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt
      241 cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac
      301 agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa
      361 aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat
      421 aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag
      481 ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt
      541 atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat
      601 aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc
      661 tgtctataat aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc
      721 cgttttctat gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt
      781 agagaaccag acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag
      841 gttagctgat cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa
      901 tgatgagcaa ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc
      961 ttttgaaaaa caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa
     1021 agaggaacag ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga
     1081 cgccagtttg aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg
     1141 attaccactt ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt
     1201 ctatcagtat tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa
     1261 ttgtgcacaa tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa
     1321 gggagcgtac actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg
     1381 gatcttgttt ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac
     1441 ctatcttgat caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa
     1501 aacacgtctc ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat
     1561 tcgaagaata cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg
     1621 gctagaactg atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc
     1681 agtcagtgga ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga
     1741 gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc
     1801 tgaaatccat gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa
     1861 aaatacagca cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga
     1921 gaagcaacag cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt
     1981 caagaaaaat aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca
     2041 attatttgat tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta
     2101 ttggacacaa tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt
     2161 cgatggaaac agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg
     2221 gttaccggaa gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca
     2281 gtcttatcca tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt
     2341 agatattgaa gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa
     2401 atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc
     2461 tagcagtatg gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga
     2521 tatgccacta gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa
     2581 cagcgatgtt tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca
     2641 tcagtttttt aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc
     2701 tttagcaatc acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga
     2761 agagactgtt cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc
     2821 caaagcactc attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact
     2881 gctagaaaaa agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca
     2941 attaaaagat aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat
     3001 tggcaccaca gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc
     3061 gggggaaaag atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa
     3121 ggaaatattc aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt
     3181 gactattcta gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga
     3241 ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag
     3301 ctgtctcatt gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa
     3361 atggaagcag tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga
     3421 agagcattat agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag
     3481 tgaagataca gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca
     3541 ctaattttgg catgcaactt gcttttttta gtatgtgtaa gtgaaaagag gagatgaaaa
     3601 agtggataga gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc
     3661 attagagctg atctatggga aaaatcattc tattactgta ttcgattgct atactgatga
     3721 gaattttgat ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat
     3781 cattgttctg acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa
     3841 tcgggatcat gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc
     3901 tcggttggac gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa
     3961 aaaaatgatc cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta
     4021 ggaggaagaa aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta
     4081 gctttttcat ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta
     4141 cctacgaatg agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta
     4201 gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat
     4261 aagttattca tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca
     4321 gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga
     4381 aaagcggtaa atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa
     4441 atagaagtag aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg
     4501 taagaaagga agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt
     4561 cgcacaatca gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct
     4621 gtttacagga atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga
     4681 gttagcattt atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg
     4741 tgggatttta gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt
     4801 actaggtttg ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt
     4861 gattccaatt atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt
     4921 cgagcatatg cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat
     4981 ggtttcttat atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt
     5041 cgttcaaaag gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct
     5101 tgctcgtttg ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc
     5161 ttcttactta gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt
     5221 ggtagtcaat attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga
     5281 agaagatgaa gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg
     5341 tattttggcg ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg
     5401 cttatgttta tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg
     5461 cagcagcttt gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga
     5521 ttttaggaat cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga
     5581 ctgcgatcga tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt
     5641 tcttttgggg aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag
     5701 gaaatatttt aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc
     5761 gctatctagc aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg
     5821 caaacggtat gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg
     5881 gagcaatgac tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa
     5941 atgctgtgac tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag
     6001 catttggcat agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg
     6061 gactagcttt ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag
     6121 aaaatgttta cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg
     6181 aaaaagaatg atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta
     6241 gcaacgggtt catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt
     6301 gatcttactg tcgatattca agaacctttt tattttttca attatggaaa actttctcct
     6361 tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca
     6421 gtaaaaatga tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt
     6481 ccacttgctt tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc
     6541 ttcgttacaa aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt
     6601 ggaataagta aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc
     6661 aaaataatca accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat
     6721 caaagtttat ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga
     6781 acagctaaag aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt
     6841 gagttagaag cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc
     6901 tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt
     6961 cggtacgttg gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt
     7021 ggattagcaa tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa
     7081 ctattcgctt ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa
     7141 gactttgata cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat
     7201 gaagaagaac aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa
     7261 atcatcgata aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga
     7321 acgtatgctt cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc
     7381 tttgtacagc atgctgcaga atattacact actggaaaca aaagattaac aaaagattct
     7441 atcgttattt tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa
     7501 atcaagaaag taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct
     7561 cagctatgcg attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt
     7621 atggcagcag atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat
     7681 gaacagctcg aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca
     7741 tttggcttat catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca
     7801 gggaatcaat ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg
     7861 cttccatcga aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa
     7921 gaaacaaccc cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt
     7981 gtggctaaac ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat
     8041 ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat
     8101 gtagtgacac aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata
     8161 cgccgctact atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat
     8221 aaagagagca gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta
     8281 taaaacacat tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg
     8341 ggtgccaacc attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta
     8401 aaagtccaag tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac
     8461 taaaacaaaa gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga
     8521 cagatttaga aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa
     8581 gtttcctaac ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag
     8641 gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga
     8701 ttatttcaag taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt
     8761 cccaacttta agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat
     8821 ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa
     8881 cattggcaga aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg
     8941 aaatcaataa gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga
     9001 ttatcggata taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg
     9061 ttccaacaaa agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc
     9121 caatcattat acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag
     9181 ttttggctga agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta
     9241 gccatgctga ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc
     9301 aaaaagcagc agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa
     9361 ataatgatgt gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg
     9421 ccggcgctat ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag
     9481 gattggcaga aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg
     9541 gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc
     9601 gtgccggtaa cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa
     9661 tggtcgttga gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg
     9721 ttgagatgcc aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa
     9781 aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt
     9841 aaatagatga tcaatagtta atcgt
//
LOCUS       DACDHJ010000087         9816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDHJ010000087 DACDHJ010000000
VERSION     DACDHJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.089"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            118..312
                     /locus_tag="IV223_001597"
     CDS             118..312
                     /locus_tag="IV223_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8711889.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            315..1517
                     /locus_tag="IV223_001598"
     CDS             315..1517
                     /locus_tag="IV223_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8711890.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2100..3731)
                     /locus_tag="IV223_001599"
     CDS             complement(2100..3731)
                     /locus_tag="IV223_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8711891.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3745..5418)
                     /locus_tag="IV223_001600"
     CDS             complement(3745..5418)
                     /locus_tag="IV223_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8711892.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5431..5748)
                     /locus_tag="IV223_001601"
     CDS             complement(5431..5748)
                     /locus_tag="IV223_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8711893.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5773..7173)
                     /gene="lacG"
                     /locus_tag="IV223_001602"
     CDS             complement(5773..7173)
                     /gene="lacG"
                     /locus_tag="IV223_001602"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8711894.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7410..8195
                     /locus_tag="IV223_001603"
     CDS             7410..8195
                     /locus_tag="IV223_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8711895.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8198..8890
                     /locus_tag="IV223_001604"
     CDS             8198..8890
                     /locus_tag="IV223_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8711896.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9164..9535)
                     /locus_tag="IV223_001605"
     CDS             complement(9164..9535)
                     /locus_tag="IV223_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8711897.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3120 a         2070 c         1714 g         2912 t
ORIGIN      
        1 agttgggaag tcctgaacaa gtgatggatt ttcaagaaag taccgttcat tatacaaaaa
       61 taaagaaata acactattac ccaatgatag tgttttcttt gaaagtgagt ttatacaatg
      121 aaaaatacaa tttgtcgaaa agatttagag gctctaggat ttaaaacaca tcaagctacc
      181 acaataatcc gacaagcaaa aacaagaatg gtaaatcagg gatatccata ttataataat
      241 aaaagagttg gggtagtacc ccgacaagta attgaagaaa ttataggtgt tcctctttca
      301 caggaggaat aactatgaaa acaaaatacc cttgtgtgta tcaagataaa aatggtcgct
      361 tttcttatct agttgagtta ggaacagatc aagtgactgg aaagaggatc cagaagaaag
      421 gaacaaaaga ttctctagga aaacgattct catcagctcg tgaagcacac aaagaagtca
      481 tgcgtataaa aaatgaatac atggttgtaa atggatttgc gaattatgat ttaacttaca
      541 aagaatttat ggagcagtct tacgttccac actacaaagc tagcgtacaa aaaagtactt
      601 ggagttctcg tgagtgtgga ttgattcaga ttactgaatt tttcggtgat aagaagcttc
      661 gtgaaatcaa tgtgcgagat tgtgaaaatt atcgaatttg gttattgaac gatagcggat
      721 actcacaagc atacagttca cttatgtacg gtatgtttcg taaatcatta gactatgcgg
      781 tcaccctgca atttctgaat gagaatattt ccaaaagaac aaaagcaatt ccaaaaggaa
      841 aatcaatcgt tccttactgg acaaaagaag aatttgaaga agttttggca aaaatttata
      901 aagatgattt ctacgaacat atgtgttttt taatgatctg gctatattac atgactggtg
      961 ttcgtgtaag cgaaggatta gcacttaatt ggaatgacgt ggacttcaaa cgtaaaaagt
     1021 tgagggttca tcacaccttg gaaatgacaa atcaaaaaga ttttattcgg aaaccttaca
     1081 caaaaactga aagcggcttg cggatcattt ctctggacga tgatacaatt gaagtgctaa
     1141 aaaagtggaa agttattcaa aaaaatcatg gtgtagaaca atttattctt agctacactg
     1201 acctcccact ctatcgttca actgttcaac gaattattca gcgttatgcc aaactagcta
     1261 aggtacccct aatacaagga aaaggattac gtcattctca tgttagctac ctaatcaatg
     1321 agtttaacgc agacattctg gtcgtgtcgc aacggttagg ccattctagc cctgaaatca
     1381 ctttaaagca ctacgctcat ctatggagcc gtaatgatga aggaattgca gaacaaatga
     1441 caggaaacat taaatttacc tgtgcaacaa aatctaaagt tgcaaacttt aatggaaatc
     1501 aagcagtaaa aatctaaccc taccatttcc ctaccaaaat gaggaatagg ctgaaaaagc
     1561 actgatataa tagcttcttt acgaggctac tcatattgag tgggaataaa gagccaacat
     1621 aatatggagg gggaatccct ataaatcaac attcttcaat agctaaaatg cttgattttc
     1681 attaaaaata agatggttcc ctaccaaaag agactataat ataagttaat ttgtttcatt
     1741 cttatcataa aaagaagttc tcgaaaaacg aactgttttc cgagaacttt tttctatcca
     1801 tctaagtgct caaatcatat tcacgacgga attctaaggt taccctttct taaacaaact
     1861 ttatgttatc tgcacaactt tttgcaattt tttcattgct tcttacgaaa tcgttgaact
     1921 ctctttagca taatacaaag tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat
     1981 gactggaaat cgtttctggt tatttttggc aaaggcgagg tgcaggttcg attcctgcct
     2041 tctacgctaa cgatgtggct gaagggaacc tccgatcttg aatcgaaacg atccgcagtc
     2101 tattgtttca ccaatgaaac aaaatcaacg ttcgtccaag catttcctga agccttgcta
     2161 taaaccccaa tttcacaaac acctgttgta attggaatgt ctttgattgt aatttttgtc
     2221 cattcacttg ttttaacaac cgcagtattt agttccgctc caccataatt tttgatatat
     2281 aactgattag tatcaaactc tcccccagac attacatagg cactcaatgt gtaagttcct
     2341 ttgcttatgc cactaacatt ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa
     2401 gttagtttgt agtccccaaa aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt
     2461 ccttgttgaa accaggtttg ccaattggaa ggactggtcg ttggtccatc gctttcgaaa
     2521 cttgcatttt taactagatt tatttgcgcc cctgcttgtt taaacgcacg caagcttggc
     2581 aaggcgtttc cagcaaaatc aaacatcgct tggttgtccc attcgtttcc catagtggat
     2641 tgaacactga gataattcat acctgcttgt gttgcccaac tcacatttcc gttgtaccaa
     2701 agaggttccc aatagaagaa gcccgccccg cgattattcg gtactgcctg aatccgatcg
     2761 gcaagatcca gcaaatactc tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct
     2821 gcttcggctt caccaaaagc atttggcata gtatcggcat tcgctgttgt atgcccataa
     2881 gctgtttcaa ctacgatcac ttctttacca taccgttggc tgatcgcatt catattggtt
     2941 tgaagttcat tcattgaacc gtgccaatac ggataatacg acactccaat taaatcaaat
     3001 gacacacctc gactcgtgat ttcatcaaac caccaagtaa atgctcctgt atcgccacca
     3061 tcagctaagt ggagcattac tggagttgtt ttaggttgag tgtctcgaac agcttggata
     3121 ccagacttca aaaaagcagc caatcgatca aattctcccc cgccttctcc ccaagacttg
     3181 ccataaggcc agagcattcc gctattcagt tcattgccga tctgaaccat atcaggataa
     3241 acgtccagct gcttcatttc cgacagaacg ttctttgtat agtcgtacaa tgccgtattc
     3301 atttcttcaa aggtcagacc ttgccatgct ttaggaagat tctgtttccc aggatcaacc
     3361 caaaaatcac tgtagtgaaa atccagcagc accttaagtc ccagattttt tgctcgtttt
     3421 gcaaggtcaa tcgtcgtttc cagatcattt gtccctgctc cataactttc tccattacta
     3481 ttataaggat cctgccacaa acgcagtcga acatagttga caccattatt ttttaaaatc
     3541 tctaaggcat cttttgaaat atcgtcttca taatattttg caccagattt ttccatatct
     3601 gacaggatcg aaatatctgc tccgcgaata aattcgctgg cagatgcttc aacagttttt
     3661 gaaacagccc ccaaagcccc taacacagct gcaaataaaa cagctttacg caaccaaatt
     3721 cccattttca ttatggctcc tcctttattg aaattggttc agaataaact gaacagcttt
     3781 ttcaggatct ctcgttaatc cgatatattc tgctcctttt gtcgcaacca gcttgattcc
     3841 taagcgatcg gtgtcttctt tgatatcatc atagtaagaa ttgacttgcg gtgctaagac
     3901 caccatatca aaatcctgca gcatttcgta atgagaacca taagcacccg ccattgcact
     3961 gatcggtgct ttcgattcag atgcaccttt ttttaaagca ttagctaaca tcgcacttgt
     4021 ccctgcgcca gcacaaagta ccaacacatt gatttcttta tttgcatcgc tgataacttg
     4081 actcgctgcc acaggtactt cctcacgtac atcggcagaa gcaccttcct cggttgcgat
     4141 cacctctgct ttttgtgctt ctttctctac taagacagca tcgtatgctt tgcaaaatgg
     4201 tagatagatc aggaagtcaa cgacgattag aaccgctgct agaacaaaag ctaagatacc
     4261 catgcccgtt cctagaacca aaccgatcgg tgctggagtt gcccaaggca aaacgtacat
     4321 aaagctgttc atgcccaata catcgacaaa aaacttgaac aggcaaacgt ttacaacagg
     4381 tgttaacaaa aatggaataa aaaaataagg gttcaaaatc atgggtgccg caaatagcaa
     4441 tggttcgttg acagcgaaac ttactggaat aaaggaagct ttcccaactg ccttcaactg
     4501 cttagactta gcaaagagta ggaagataaa aggaacaacc aatgttgccc cagttcctcc
     4561 catagttcct acaaaattcc ctaaacccac cgtcagtact ttagaagctt gttcaccgga
     4621 attgaacaat tgcagattgg tttccacatt ggcatagata attgccgcaa ttgccggctc
     4681 cacgatcgac ggtccatgaa caccgacaaa ccaaaacagc gccatcgctc cccagataat
     4741 agcgattcct aagtacccgt cagctgctgt aaatagcggc tgcagcaccg taatgattgc
     4801 ttctgcaaat gagatttgga agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa
     4861 tactgaaaaa gagaaaggaa aaatatctcg gaaggtttgc gaaatcgttc caggaacttc
     4921 tttaggcatt ttaatggtaa tatctttcag cacacaaaac ttatataaat taactgttaa
     4981 aaatgcagcg acaaaggaag ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc
     5041 accttcaatc ggagagacgc ttaacaacag gaaacttacc acagaggcca gcatcacaga
     5101 ggtggtgttg attactttgt ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg
     5161 agcagtggtt gctgaaacga gtaaccctac aacacccatc gaataattat agactttcca
     5221 tagccatgtg gagacagatt caggccattg gtaaccaaaa atctcaggaa ccgcagcaat
     5281 caaaatgaaa atgcttgaaa ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac
     5341 ggctcctaaa taactattac gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg
     5401 tttaatgatt ccatccatag cgttcttctc ctatccttga ttctgtctgt acagtacaat
     5461 aaagtcttga atcagatctc tcagcaataa agtggtcatt aaatgatcct gcccatggat
     5521 aaaaatgaat cccatctcca tctctttccc agatgcttct tccgctaaaa tttttgtttg
     5581 cgaattatgt gcaagggtta aattttcgtc ggcttctttc aacgccgaat caacatgttc
     5641 aaagtttcct tgtcttactt ctttcaataa tttcaataat gtactgcgag cgtcgcctga
     5701 atatgcaacg atctcaaaac caagcatttg taattcctct ttattcatct tttcttcctc
     5761 cattatcact tattaaataa tcgttttggt ttcactgacc aacttgtacc agtaagctga
     5821 ttcttttggg taacgcttct gggtttcaaa atcaacataa aacaagccat accttttgtt
     5881 atacccattt gtccatgaga aaagatccat aagcgaccac aaaaaatacc ccttcacatt
     5941 taccccggct gttatcgcct tgcttaagga ttccaaatag actctcaaat agtcgattct
     6001 cggctgatcc ataataatgc catcttcaaa ctgatcttta taccccatcc cgttctcagt
     6061 gatatagatt ttgttgtaat gtgggtaatc gcttttaatt cgcagcaaca agtcatatag
     6121 accttctgga taaatcagcc agtcccaatc agttcgagga attccttctt tataaatacg
     6181 ttctccgatt cccttcactt tataaacaga ggtccctttt tcacccgtac cgttatgatg
     6241 gattgcattt tctccatcgt aagctttgac aaaatggcat tggtagtgat tgatccctaa
     6301 gtagtcatta cgcgtggatg cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata
     6361 gctggcctga tttgcttcac atatctcatc aagagcagtc aaggtttcag ttgaataata
     6421 gcctaaatag gtagcatcta ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc
     6481 atgcttatct tccggcgcgt ctgttgccgc atatttcgtc tctaatgagt gaactacacc
     6541 aatttcaccc ggaagttcgt tttctttgaa atagttcact acacgggcat gagcaaccat
     6601 catattatga agacaggcga cgatctttgt aaaatcatat ttgattcctg gtgggaaaac
     6661 acctaacaaa tattgattgg ttgcaactgg atagatttca ttaaaggtac tccacacctt
     6721 gacttctttg aattcatgaa aacaaaaaat ggcataggaa acaaatgctt ctattgtttc
     6781 tcgattcaaa aaatctccat ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt
     6841 cacaaacggc gttacgtttc ttttcttaca ttcttcgaac acacgatgat aaaaagcgat
     6901 tccttctgga tttggtttcc cagctccatc aggaaaaatg cggctccaag caatggataa
     6961 tcttagacca tttacaccaa aacgttcaca caattcaatg tcctttgcat attgatgata
     7021 aaaatcactt gccggatcgg gactgaatcg cccttgttcc tctaaaaagt catcccaagc
     7081 aacagcgcct ttgccacctt ccttagtggc tccttcaacc tgataggctg ctgtggctcc
     7141 gccaaaaata aaatcttctg gtaatttaag catgtacatc tctcctcaaa tctataatca
     7201 gttttcgctc tttctaactg attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa
     7261 caaacacaaa taatcataaa aaccagaaaa cagcaataaa atctattgta acaaacataa
     7321 aaaccaccct aaatgtttac ttttgttcga attagattga ctgttgtccg tttatatgat
     7381 agattatcct taaattagag gtgaagacaa tgttgaaaaa agaaagattg cttactatcg
     7441 tagaaatggt caataaaaaa ggaatcctta ccgttaatga aatcatcaat caattagatg
     7501 tttctgatat gaccgttcgc agagatttag atgaattaga aaaatcgggc aagctgctgc
     7561 gtgtccatgg aggggctcaa agtctttcct acactttaga tcaagaactt tctcacactg
     7621 aaaaagccac tctgcagatt caagaaaaaa aagaaattgc tgccacagca gcctccttga
     7681 ttcacgaggg agaaacgatt ttcttaggac caggtacaac aatcgaacta ttggcgacgc
     7741 atttactgaa caagaaaatt cggatcatta caaataatta tccagttttt gatattcttg
     7801 ctcaagcaga tggaccagaa attctactga ttggcggtga tttccggaaa aataccggcg
     7861 catttgtcgg accgatcacc aacgacaatt taagaagact aaagtttaca aaagcattta
     7921 tcagttcaaa tggcgttcac aacgaagcga tctctactta cagtaccgaa gaaggagaag
     7981 cacagcagat cgctctaaat aattcccgta agaaatatct actcgttgat aataaaaaat
     8041 tcaatcgaga tgatttttat gtgttttata atttgcatga tttcgatctg ctcattacgg
     8101 atcgccagat ctcgcgggag gttcaagagc actatgcaca atatgtcgat ctgcaaattg
     8161 ctgattcaac cgaaaatacc actataggag cgtaactatg aaagggatta tatttgattt
     8221 caacggaaca atgtttttgg actctcatct gcatgaagct gcttggctgc atatgataca
     8281 tgatcataca aaagatggtt tatcagacga ggatatttta aaaaatattc atggtcggac
     8341 gaatacggag attttgaaac atttcatttc agagcatttg accgcctctg agatcgccgt
     8401 attgtcagaa gaaaaagaaa gctattatcg tatgctttgc ttgcaaaatg aaaacgagct
     8461 tcagttgaca aaagggcttg aagcgactct tgaccagctg actgaccaac agattccatt
     8521 aacgattgcc acggctactg tcaaagagaa tgttgctttt tattttgata ttttcgattt
     8581 agcccgctgg tttgactggg acaagattgt ctatgacgac ggctctttcc ctgggaaacc
     8641 gcaaccggat atttttctta aagcggcaga caaactgagc ttagctccgg ctgattgtct
     8701 tgttatcgaa gatgcttact caggattact tgctgccaaa cgtgcgggga tcggaacaat
     8761 tattgcaatt gatccattcg gtaaaaacaa aacagtattt gaacaggcac agctaaataa
     8821 agatggaacc atcaaagatt ttactggttt ttggacagag cacctgtctg ccacatccgt
     8881 taattcttaa acgattcaac taactaccaa agaaaagcaa ctgaaatgca cacctccttt
     8941 tataaaaaac tgccagtgaa acgacatgaa agatacactg ctatatcctt agtgaaatat
     9001 agcagtgtat ctttgacttt ttcattcttt taaccatatg acgcgttcat ctctatctat
     9061 aattaacatt aaaaatagtg ttttcacatt atttctcaaa ccaatcttaa aaatcatcag
     9121 gagtactttt ttgatatact tttattaatg ctgggcgtaa tggttaaaag atttccacta
     9181 gttcctctcc accaagctga gttgaaaaat gcttatctgt aaacaacata gacaaagagg
     9241 aaataaacgt ttccgtatct ttctctgtcc caaggatatt aattaatttt cctactaggt
     9301 aagtatcatt aaacttaaga tctgccttca catcaaaagt tttgataaaa gcttttatca
     9361 tgcgtacaat tttgtcttta tcgccataat tgatttcacc actcatatca atatagtcac
     9421 taatccctga agaaataatg gcaactgctt cttctttcgc cttatcagat aattcattta
     9481 atgactggga ttgattgatg acttcttgag ttttatctgt tacgtaatct ctcattatcc
     9541 tatcggcata ctccgcaaag taatcttgaa aaaattcttc tacagggtaa ccaacttcgt
     9601 tttcccaacc aggagaaatt tcattatcag tacttattaa atctattaaa aaccaacccg
     9661 ttctctgagt gttgattcta taatattttt aatttcctat ataaatcaaa aatattatag
     9721 aatcaataag ctaattaaga aacaaacttt agaaactcta gaagaacttc tgaatttata
     9781 tagaagtgtg tagctactta ctaaatatac aaaagt
//
LOCUS       DACDHJ010000088         9736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDHJ010000088 DACDHJ010000000
VERSION     DACDHJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.090"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..1068
                     /locus_tag="IV223_001606"
     CDS             37..1068
                     /locus_tag="IV223_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8711898.1"
                     /translation="MIAELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQG
                     TIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQ
                     DAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGI
                     VDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQ
                     LVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKAN
                     IKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLF
                     QQGRQQERE"
     gene            1079..3088
                     /gene="topB"
                     /locus_tag="IV223_001607"
     CDS             1079..3088
                     /gene="topB"
                     /locus_tag="IV223_001607"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8711899.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3434..4030
                     /locus_tag="IV223_001608"
     CDS             3434..4030
                     /locus_tag="IV223_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8711900.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            4043..4942
                     /locus_tag="IV223_001609"
     CDS             4043..4942
                     /locus_tag="IV223_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8711901.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKDGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4945..5076
                     /gene="ssb"
                     /locus_tag="IV223_001610"
     CDS             4945..5076
                     /gene="ssb"
                     /locus_tag="IV223_001610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8711902.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5098..5430
                     /gene="ssb"
                     /locus_tag="IV223_001611"
     CDS             5098..5430
                     /gene="ssb"
                     /locus_tag="IV223_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8711903.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5452..5709
                     /locus_tag="IV223_001612"
     CDS             5452..5709
                     /locus_tag="IV223_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711904.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5868..5990)
                     /locus_tag="IV223_001613"
     CDS             complement(5868..5990)
                     /locus_tag="IV223_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8711905.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6180..6404
                     /locus_tag="IV223_001614"
     CDS             6180..6404
                     /locus_tag="IV223_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711906.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6847..7053
                     /locus_tag="IV223_001615"
     CDS             6847..7053
                     /locus_tag="IV223_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8711907.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7053..7304
                     /locus_tag="IV223_001616"
     CDS             7053..7304
                     /locus_tag="IV223_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711908.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            7485..7757
                     /locus_tag="IV223_001617"
     CDS             7485..7757
                     /locus_tag="IV223_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711909.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            8202..8381
                     /locus_tag="IV223_001618"
     CDS             8202..8381
                     /locus_tag="IV223_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711910.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            8440..8796
                     /locus_tag="IV223_001619"
     CDS             8440..8796
                     /locus_tag="IV223_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711911.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         3500 a         1618 c         2007 g         2611 t
ORIGIN      
        1 ccagagtggc tgattgatta tgcagaaaaa gaagggttga ttgctgaact aaagccgaaa
       61 catgaacggc aaaatttttt agttggagac gaccgtttag atcatgcggt tgctttttta
      121 tggaaagatc cacagacagg agagacagta ggggcaagtt atcaaggaac gatcgtcgat
      181 tttaatcgtt tcggtaaacg gggaacgtat aaacacattg acaagaatcc tacgccaaat
      241 cacggattta atttaaaaat aggcgatccg aagcatttaa aattctttga aagtagtatt
      301 gatctgttaa gttacgctgc attgaatcgg gagaaactac aagatgcctg gttagtttcg
      361 atggacgggt taaaacatca tgtaatcagt cattacgttg aagagtcgat ttctgaatta
      421 agacgaaaac aaacatttcc tcaatcgatt gaaatatgtg tggacaatga tcgtgcagga
      481 catatttttt atgaaaaaga acagatgaag ggaatcgttg atccttttac caataagaaa
      541 atccgttgtg agcgaggaat tccaaatgat tggcaagtac ctaaagaata caaagctact
      601 tatgaagcag tcgcaaaaga aatgagtgtt gaacctgaag caattatggc catacacaaa
      661 actgaaacta atttacagct gacaaatcaa ttggtgtctg ctcatgatgt tcaatctacg
      721 tttggaaaga tgttggcgaa gggcgaacca gtagaaacaa ttgatttgaa agaagcatgt
      781 accacggtcg cgaaggaact gaaagtatgt gaacgcgcag atggaacata caactttgat
      841 cgtttttata gtagaaaagc caacataaaa gacgttaatg caggaatcct tctttcctat
      901 aaagcagaac aatactacaa aggctataaa aagcatgaac atgaatttgt acctgaagtg
      961 aaaaaggatt ggaatgatca attgaagcat gagatccagc aacaagaaat cagaaagcaa
     1021 aaacgtgcga tgttgtttca acaaggcaga caacaagaaa gggagtgaag tcgtcactat
     1081 gaaaaccgtc attttagcag aaaaaccgtc acaagcgaag gcttatgccg atagcttttc
     1141 taaagctacc cgaaaagatg ggtattttga gatccaagat cgattgttta caggagaaac
     1201 tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata tgtacgatga
     1261 aaaatggaaa caatgggcat tagaacattt gcctattttc cccaatcaat accactatca
     1321 tgttcctaaa gataagaaaa agcagtttag tgtcgtcaaa cgacagctgc agtcagctga
     1381 tacgattgtg atcgcgactg atagcgatcg tgaaggggag ctgattgcct ggtcaatcat
     1441 tcaacaagca ggcgccaatc aaggaaaaac ctttaaaaga ctttggatca actccttaga
     1501 aaaagaagcc atttatcagg gattccaaca gttacgagac gcagaagaga cctatccaaa
     1561 atttgaagaa gtacaagcac gtcagatcgc cgattggtta attgggatga acggtagccc
     1621 actgtatagt ttactcttgc agcaaaaagg cataccagga agtttttctt taggtagagt
     1681 ccaaacccca acactctata tgatttatca attgcaggaa aagattagaa acttccaaaa
     1741 agaaccatat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat ttgatgcgaa
     1801 gcttgatcca aatgaaaccc aagcgacaca agaagcattt gaagcctatt taaaagaaaa
     1861 aggtgttcaa ttaggaaaac agccaggtac gatccttcaa gtcgaaacag agaaaaaaag
     1921 tgcggctagc ccacgtcttt tttctttatc gagccttcag tctaaaatga atcaactcat
     1981 gaaagctagt gccaaagata ctttggaagc catgcaaggg ttgtatgaag gaaaatatct
     2041 aagctatccg cgaacggata ccccttatat tacggaaggg gaatatgcgt atctgttgga
     2101 tcacttagac gaatacaagc attttttaaa agccgaagcg attccgactc ctattcatac
     2161 accgaatagt cggtatgtga ataataagaa agtccaggaa cattacgcga tcattcctac
     2221 aaaaacggtt atgacagcag cggcttttga acaactaagt ccactgcagc aggctattta
     2281 tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aagtatacct atgaagaaac
     2341 aacgatcttg acgcaagttc agcagcttca gttaaaggca atcgaaaagg tgccgctaga
     2401 tctaggttgg aagaagttat ttggaaaaga gagcgacgga aaagagaagg aagaagagcc
     2461 gctacttcct aaagtgacaa aaggagaaat ggtgacggtg gatctacaag ttcttgaaaa
     2521 agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcaa tgaaaacagc
     2581 tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg aaggaatcgg
     2641 tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat atatcaaggt
     2701 agagaaaaat aagctcgtgg tgacgaataa agggattttg ctttgccaag ccgtggaaaa
     2761 agagccgctg cttacgagcg ccgaaatgac cgccaaatgg gaaagctact tgttaaagat
     2821 cggtgaacga aaaggaacac aaaccacttt tctagccaat atccaaaaat ttgtctctca
     2881 cttattggaa gtcgtaccag ggcaaatcca atccactgat tttggatcga cgttacaaga
     2941 agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt gtccaaaatg
     3001 ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg aagcctgtga
     3061 ttttaaaagg tcagaagggt tcatttgaag caacgattgt tttaaaagaa gacttcacta
     3121 caggctttga attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgccgcactg
     3181 caaaataatg gagggttaat cttatgcgta ataccacaca caaacaacaa gaaatcgaaa
     3241 atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg tgccagcaaa cgaagactgc
     3301 agcaattgaa gaaccagcag aggattcaaa aagaaatcga attagatcga taaaatcttc
     3361 ctaactattg tatacgtata aacttatgtt atactgtttt tataagttta tacgtataca
     3421 aaggagggag aaaatgacaa aagcaatggt atacaagctg ctagaagaat ttaatggaac
     3481 tttggctttg aaggatgcca aaaaagcggg catttctcct gtaacgatca aacgtatggt
     3541 agatcgaggg gagctggatc gagagtatcc aggttttttc accttacctg gtcaatttcc
     3601 tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc attatttcac atattaccgc
     3661 gttagatttg tatgatttaa cagatatgat ccctagacag atcgatctga ctgtacctta
     3721 cggttatcac gtttctgaaa aaggattgaa ggaattcgca gttgagctac actatacaaa
     3781 agcggaatgg tatgaattag gtaagattga aataaaaagt cgctatggga atcctattat
     3841 agcttatgat ccagaaagaa ctttatgtga catttggaat ccctggtata ccgttgagga
     3901 tgaaatcaaa gtaaaagcaa tcaaaaacta tatggagtcg gacagaaaaa atcttagaaa
     3961 attgaatgag tatcgacgaa ttttaccaac agacaaaacg atgcgttctt atattatggc
     4021 tttaaattaa aggagaagat acatgacacc tacgcagtta aaagcaaaag tgaagaatat
     4081 ttctagagaa aaagaagttg atccgcagct tgttttaaga cattttatga tggaaaaatt
     4141 cttggaaaaa atctcagagt ctccttatcg tgaaaatttt gtgttaaaag acggcttctt
     4201 gattggctca aagtatggca ttgaaaatcg cacaaccaaa gatattgata cgactttacg
     4261 ggaaatgaaa gtaaccaaag aaacattaac aacggttcta aacgaaattt tttcaacgcc
     4321 gacgaaagaa ggcattcaat ttgagattca aggaatgaaa gaaacgagag aagccgatta
     4381 ctatccagga tttagcttac gtgtgctggc acatttggaa aacatgcggc cagattttaa
     4441 ggtagatgtg actacaggtg actcgatcta tcctgcaacg atcactcaca gtcataaatt
     4501 gatgtttgag gatcgaacga ttcctttgga atcctatcct acagaacaaa ttattgctga
     4561 aaaattaagt gcaacctttg attttttaac agataattcg agaggaaaag atttttatga
     4621 tctctatact attcctaaaa tggagaagat cgatgagaag accttgaaag atagtgtgag
     4681 aaatacattt acacgtcgag gaaaaactga tccgttaaag atatactatg aaaaggatat
     4741 ggatgtactg aaaaatagtc acgagctaaa agatacctgg aagaaatatc aaaaaagcaa
     4801 tccttatgct gcgtcgatca cgtacgatga gacaatggat tcgattagta atttgatgga
     4861 aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct tatcaaagac accaacagat
     4921 gaatagagaa atggagagat aaagatgatt aataatgtga cattagtagg acgcttaacc
     4981 aaagatcctg atttacgtta taccgcaagt ggtactgcag tagcgacttt taccctagcc
     5041 gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag ccgattttat taactgcgtg
     5101 atttggcgca aaccagcaga aacaatggcg acattagcta aaaaaggtag cttgattggt
     5161 gtagttgggc ggattcaaac gcggacttac gacaaccagc aaggacaacg tgtatatgtg
     5221 actgaagttg tagcagataa ctttcaacta ttagaaagta aagcagcgac agagagccgt
     5281 gcgcacgctg atcaatcgtc caccagtcca agcaccgcca cctttgaaca acggaataca
     5341 gcgacgccta acaacaatgg attgaacgct tcacagaatc catttggtgg acaatcaatc
     5401 gatattagcg atgatgatct accgttttag tttgagaaag aaggagtaag catggcaaaa
     5461 aaaattagtc tgacgatgga tctattgata cccgtaattg catttataga cgggatttct
     5521 ttgggcgtca ctgaaaatcc tttggtaaat agaattgcga aaggatcact atttcttgta
     5581 ttcgtgtatc ttgtctgttc ttatctttta aaaagagcag gaaaggataa ttccttttgg
     5641 cgattgacta aagtcacagt agcagcgaca gctgttatac ttacggctat tctaccgctt
     5701 tatatgtaga gttaacggga aatcgttgaa cttcttgcga aatattgcta taatgaacat
     5761 gaaaaagagg agttgcgcta acaacccctc attaacagca ccgattaaag aacggtggca
     5821 gaatattagt tatttggaaa taaccgctga cctgctaaag tagacggtta tttctttttg
     5881 tcatttttaa gcatatttac aatcaaacta atcaaagcaa tggtaaacat accaaaacca
     5941 agaatggttt gaattgtctc atatgcggac aaaaggctta ctcctttcat agattttagc
     6001 acttacattc ataagcacca ccacctttcc agaagtcgcc accgtcttca aactttactg
     6061 tcttgactat tatagcatat ctaaaatcga ataaaaattc tcaattttag ctgtttttgc
     6121 tataatttta gcgaggtgat cgtgatggaa caatatattg gtttactcga taaattaggg
     6181 tgatcaagac acagccattg cttgtgcgat ttacattacg cacgagtcat gaaccaatca
     6241 attgtgtagt ggccaatata gaaatagcgg ataaattgct gattttggaa gaaggcaagt
     6301 atactatttc agtgacaggt cacttcaata aacgaaagca actagtcatt gaatcaatgc
     6361 aaattagaaa ccctgatcat tttacgcgat cgatgagcat ataaaagaag agctgcagtc
     6421 ctaaatgagg attgtagctc tttttgtttt acatagaaac agtaaaaatg aaaaggcctg
     6481 tttaggaagg cacaagcgcc ttctcaattg aaagaccttt ctctcactct aaccgccacc
     6541 gccgttcgag aagaaaggaa gtgaacgcta cgcgttcact aagataaagg gtcttgcata
     6601 gcaagacaaa atagaaaaaa tttcctctca ccaaaaggtt cgctcctgtt cgttatctct
     6661 ttattgattt gtcagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     6721 gggtaaatgg cacgctgcgc gcccttgtct tgtcgctttt cgttgattca ttctggccaa
     6781 caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa
     6841 tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac
     6901 tatcatgcac cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc
     6961 tattgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattat
     7021 attttaattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgactg
     7081 taaaagaagt cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag
     7141 ttttacttat tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta
     7201 tgctatttga agacctagtg aagattgcag gatatgaact gcattatcaa gaaaacttgg
     7261 aaacttatga aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata
     7321 tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa
     7381 tggaggatat gacgttccta tacaacgaaa cacgtgttcc aaaaaaacat tataaagaaa
     7441 aattgtcggt agctgtggaa gaaatgattg aataaggtgt cgaaatgaat cttattgcga
     7501 cgtactatcg aacactggaa gaactgaaaa agcaaaatgc aaaatggttt ttccaagcct
     7561 tactatgctt ggaagtaggt gtcaaaccaa gcactattaa accttctgaa taccaagctt
     7621 tagaactaac gtatgcgaaa tttatcgaaa cgaaaaaagc caagactgtt tcaagtgaat
     7681 ggttagatta ttttgaaaat atcaataagt atggcgcata ttacaccatg aaaaaggaag
     7741 acaacgaaaa tgaataaggt gatttatcaa ctaacaacgg attccatatc aacgatttga
     7801 aagaaaaaaa ggtcaataat catttatacc gaaaagtgag aatgtgtgaa acaaggacaa
     7861 aaaaagccta gtaactaagg aaatggttct tatttcgcac caatcgctcc aaccgccccc
     7921 gccgcttagg tggtgcgaaa taaacgggca aagcccgata acttaagaga aagaaggtga
     7981 tccatttgaa caagattatt cccttattgt tagtactgtt tgttggcgaa atacttatgc
     8041 gagatagtgc agctgtacag ttaaacctcg ctgtattttt ggtaatactg gtgttttgtt
     8101 tgatcttttg gcggtttgta ttagtcctcg tgagtctact attcaagatg attgcagcca
     8161 tatttttcat agggttattt cgatgaaagg agtgagtacc aatgccaatt gcaataggaa
     8221 ataaacggtt acccgtgaca ttagatgaaa agagacaaaa agaattgcag caactaaagc
     8281 agaagtatgg caaaagtgaa tctaagatta tgtgtattgc gttagatcta ttgatcgcgc
     8341 aagaaaaagc aggatttgat gtaccagcgc taaaaaagtg acgtcacttt ttaatttgat
     8401 ctatataagt gatatcactt ttaccaagga gggaattaaa tgttaaaaac taaaaagaat
     8461 tcgtacaaat taaagcttag tatactatct attatttgtc ttttaggcat gataattagt
     8521 ggtcatttgt atagagtggc atatacgcag tttattgagt acaaggttca ctctttagga
     8581 ttagctgggg atcgatacgt tgaatatgtc cattattcat taaaatgttc ttcatatatt
     8641 cttctttggc ttattttttc attgtttgaa ttgctaaatt taatagcaat atcttcatat
     8701 cacttagata agctaaatac caaatctagc cgttataacg aagtgtttaa ggaaaatgtg
     8761 aagttagaaa gtgacttaaa caagctaaaa aaataaaaag aagaaaacat aagttaacta
     8821 aaattatttt attaagaatg atggaatata aggataaggt taccaacata acgatcatta
     8881 cttctctgat atgctatgga cttctatcaa tcagtatttt gtctatagat tatggagtaa
     8941 ctatactgca agccttagtt ttggctatgc ctatgtatct atctttgttt atcatgcatg
     9001 aaatatataa gctatttgtt ttatcaaaaa atgtagaaat acgggtaaat attttggtga
     9061 atagttatca aaaagaactc aaagaaagaa gacggtggcg gtatttattg aaaggatgaa
     9121 aaagtaggat ttgacatact ggtattcaga aagtgccgtc acttttaaaa caatggaaaa
     9181 ggtggagata agaggaagaa aaaacaaact aaactatcat tagcagatat ttaacacagt
     9241 taaccgcaac tgaagatggt gttgttgaat ttgaacaatc tgaaattgcg gtagtagatg
     9301 atcgttacct taaaatgcct tacttctttg accaagcaaa ggtcatttgc ctttgtcact
     9361 acgatggggt acgtgatttt aggcgttaaa aagtagtgat tatatcaact ttacgagtaa
     9421 atataaattt agacatgaaa ctgaattatc caatggaaaa tcatggttta taatctagaa
     9481 aaattatagt aatgattgga ttatatatat tctttcgtat tttcgtacaa ctgtagcttg
     9541 tcaaaattga gaaatttaac cgattaggta ctttacatcg ttatttgggt aatttctaaa
     9601 acatgaagct taccaacgaa taaactgaga ctatcttatt aattgatcaa tataagaaaa
     9661 tatctttgag gagatgatgt actatgtttt cgtctatcca cgattggtta ggtggtcttt
     9721 tcggcggtgg cggtaa
//
LOCUS       DACDHJ010000089         9641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDHJ010000089 DACDHJ010000000
VERSION     DACDHJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.091"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            179..1177
                     /locus_tag="IV223_001620"
     CDS             179..1177
                     /locus_tag="IV223_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8711912.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1289..1798
                     /locus_tag="IV223_001621"
     CDS             1289..1798
                     /locus_tag="IV223_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8711913.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1828..2487)
                     /locus_tag="IV223_001622"
     CDS             complement(1828..2487)
                     /locus_tag="IV223_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8711914.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2489..3253)
                     /locus_tag="IV223_001623"
     CDS             complement(2489..3253)
                     /locus_tag="IV223_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8711915.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3261..3938)
                     /locus_tag="IV223_001624"
     CDS             complement(3261..3938)
                     /locus_tag="IV223_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8711916.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3975..5360)
                     /locus_tag="IV223_001625"
     CDS             complement(3975..5360)
                     /locus_tag="IV223_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8711917.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5570..5654)
                     /gene="ffs"
                     /locus_tag="IV223_001626"
     ncRNA           complement(5570..5654)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV223_001626"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5677..6192)
                     /gene="tadA"
                     /locus_tag="IV223_001627"
     CDS             complement(5677..6192)
                     /gene="tadA"
                     /locus_tag="IV223_001627"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8711918.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6299..7156
                     /locus_tag="IV223_001628"
     CDS             6299..7156
                     /locus_tag="IV223_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8711919.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7237..7572)
                     /locus_tag="IV223_001629"
     CDS             complement(7237..7572)
                     /locus_tag="IV223_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711920.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7639..9009)
                     /locus_tag="IV223_001630"
     CDS             complement(7639..9009)
                     /locus_tag="IV223_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8711921.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9087..9623)
                     /locus_tag="IV223_001631"
     CDS             complement(9087..9623)
                     /locus_tag="IV223_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8711922.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2822 a         1991 c         1618 g         3210 t
ORIGIN      
        1 atacttcata ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt
       61 cttttatttt tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac
      121 atttttgaga agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat
      181 ggaaatctat gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta
      241 cgcaggaatg cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca
      301 attagcaact ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa
      361 agcaagtgaa ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca
      421 tttaaaagaa gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa
      481 taaaggcatc cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc
      541 aagaaaattg aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt
      601 gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc
      661 tatcgattgg acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg
      721 cccggaattt cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt
      781 gcttactccc tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg
      841 gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa
      901 ttatggattc tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa
      961 tgccattact gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga
     1021 catctgtacc tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac
     1081 tgcagtgaac aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag
     1141 cacaagctta tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc
     1201 aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag
     1261 aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa
     1321 ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat
     1381 gtttttacaa aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa
     1441 cagcgtgcta acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat
     1501 attagcagaa aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg
     1561 cctttacggt atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg
     1621 ctttacgaat tatggttata tcgacaaggt gaaacctggt attttaaaag aactaaacag
     1681 ccacgatggc gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc
     1741 tgctgccagt cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt
     1801 caacaaaaaa agaaagaaaa cgtctattta gacgagttct ttcttttttg tttgtttcaa
     1861 gctatttttt cctaagaaaa gaaaaacagc aaaaaataat tctaataata caaaaaataa
     1921 gataaagcta tgcatgaaaa attcttctgg aagtacattg atgattggat cagtaacggg
     1981 atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc
     2041 aaatcctatc atcatgaaaa aaccgaaaac aactggcagt aacatgccga tttgaaacgg
     2101 acgaatcaac cgccaaagcc gatcattctt ttgcaaatat ttgataaaca aaatactcgg
     2161 gatcaaagtg atgaaaaaca cagcataatc cactaaaaac aaaattttca cttcataaaa
     2221 gtgatgggct ccgctagcag aaactgggaa atctggcaag ctgaggatgg ttttgaaggg
     2281 attattgaga taattcatta ggtgatcaaa atttttcagt aacgtctctt gatctaaaga
     2341 agtataatcc aaaatttgca aatgtccgat atcaaatacg tatagtggtc gaaagttgat
     2401 cgtcacacca actgccagac tgatcagtgt cagaaatagg ctgaacaatc cagcatattc
     2461 catccactgc catctcgaat ttttcatatt agaagctcca ttcatccagt gattgtttca
     2521 cataggtagc tgggataggc aacccaggta catcttcttc ttttgtaaaa cctgataaaa
     2581 cgagcaaagt atctatatta ttgcgtatcc cagcttggat atctgtttca tagttatctc
     2641 caaccatgat tacttcttct ttttgcagcc ctaatacttc aacggcttta tccataatga
     2701 tggcttctgg ttttccgatg tagatcggcg gcgtttgggt tgctgtctct acaaaagaga
     2761 tgactgaccc tgctccagga agaaggccgc gttcagtagg tatattctta tctgggttcg
     2821 ttccaatgaa agtagcacct ttttgaatag caagtgttgc tttaactact ttttcatagg
     2881 tcagataatt atctaagcct accactacat agtcaggagc ctcttcttcc catgtaaatc
     2941 cggcagctaa aatcaagtca atcaatcctg cttctccgat aacatagact tttttccctt
     3001 ttccatctgc tttcataaaa tcgattgttg ccaaagtagc tgtgtagact gtctctggag
     3061 ctacatgaat gtcaaactca tctgctagac gattagctac tgtttctggt gacttagtcg
     3121 tgttattggt aacaaacaag aaaggtaatt ttcttttttg caattcttcc acaaatcgtt
     3181 taccggccgg tatgggttgt gtcccacgat aaatcgttcc gtctaaatca attaaatatc
     3241 ctttatagtc cattcatcca ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt
     3301 ttttctactt gtttaggttg ttcttgtttt tttacttttt cagaggtttt tgctgactct
     3361 gtttttcgat ttttaataac cggttttttg cgattagatg cgactggtcc ttttttctca
     3421 tcgatatacg cacgattatg gttttttttc ttttttcggc gctgagttgt cttttctttt
     3481 ttaccgccga ttctctcgat gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga
     3541 tagtcttcca aggaatcgat tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa
     3601 aatcctttta ggcgtagttg ttcataaccc caatctccta cgatataatc atatcgggct
     3661 agtacatcac tgtaacgttc tccaagcttt tctgcattaa atccttcacg ataatcggac
     3721 acaagccgat aatgtctttc acctatcaaa aaattactgt catctaacag tgtcactagc
     3781 tcgcctttga ctttttctgc tttaggctcc tcaacgattt cttccaaagc agctgtcagt
     3841 tcttcggtga ctgttgtttc ctgttcagct gatgattttt tcttagcaat ttctgtttta
     3901 tgctcaacgt tctgctgttc attttgtttt gcagacatgc gttcaccacc tatctttttt
     3961 attatacctt gtttttattt gattgggtag tgagctttca agtaatctcc taccacactt
     4021 cggataaatt cagggaacaa aaattcattt tttccagcaa tctcaatcgt tggaaaaaat
     4081 ggaacaaaca tcaaatgatc tacggtagta aaagtataat ttttattcag atcgattggc
     4141 ttgttcttcc aaaagacttg atggttaata gaatcatatg tgattccatt atagaccatc
     4201 tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag gatagttacg taaaaagtta
     4261 cgattttttt ctatttctaa aactaagcgg atcagatcat ttccacccaa tgtgacattt
     4321 aacaagtgca tcggatgcgg caacgcagtg tgaagctgat cctgattgac ccgccctttt
     4381 ggaatttcag ttaaaaacag accactgttt aatattgctg cttccgtctt tcctcgttct
     4441 ttcaccgcct caaaggctac ttggataaac gaatgctcat tgaagatatc tagactcaga
     4501 gaaaatggca aatctgctac ttctttttct tttaataatt catgcccacg ttctagataa
     4561 ctatttattt cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg gatggctttg
     4621 gctgaatgct taaggatttt tttctcttca tcgatggtta aatggacttc accgacatac
     4681 tgcccgtact ttccagcggc tgctatttgg acatcattga ttacgtgacc ttctctgaat
     4741 aaatgatggg tgtgcgatcc taaaataaca tcaattgctg gtatttcttt tgcaatttgg
     4801 aaatcatcct gaatcccgag atgactcatc aaaactaaaa catccacttg tgaacgtaaa
     4861 tcttcgacta attctggcaa aatatcataa ggattccgta tgtcccagcc attcggatta
     4921 taagtaagcg gaaatggagc agtaaatgca attagtccga ttttcgtctg gtgtttcgtt
     4981 gtaatgattt tatatggttt cgtccatgcc ggtggctgga gcgtattttt atcgaataag
     5041 ttatccagga tgatatcaaa ctcagcatga tcgtataaat gattcaactc ttctttggaa
     5101 tttcctacgc cttcattgtt tcctatcgtc gctgcatcat aaccaacttc attcatcagc
     5161 aagatatttt cctgtccatt cgttgcttct gacaatggat gccaacgatc cacaaaatca
     5221 cctagatcta ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg tttacgttga
     5281 tccaaaaacc ttctgatcct tggccaattt tctaaatgag aatgcagatc attcgtatgt
     5341 aaaatcacta ttttttccat gtaccctctc gcctctcttt atctttatca taccataggc
     5401 agctttttct tccattgaaa caagctctat ttcagagttt ttcctgcctt gaagcaaatt
     5461 gtataagtac aatattcgat aaatcagtct ggctaaaaaa tcaggaacat tcatttttac
     5521 agtactataa aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat tataatcaga
     5581 cacatggcgg aacctgctta gtgctgcttc cttccagacc tgacacgctt cacaagccca
     5641 ccattgtcct agcctttcgg caaagaatag tataccttat ttttcattcg ttgaccagtc
     5701 tttttttaat ttcttttttc gctggcggag ctttcggaaa aagtcagtca aaatcatccc
     5761 acattcgtct tctaaaatgc caccttctac atatgcttgg tggttaaacc gctcgtcctc
     5821 caaaagattc atcaaggtac cggctgttcc gccttttgga tcatacgcgc cgaaataaac
     5881 ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac
     5941 aaaaagtgca gcatcttcca agcgccagct tcccactttt tcacaggctt ccttgattgc
     6001 aagcatttct gcatgtgtag tagcatcttg cgtggtttct ctaaggttat atcctcttcc
     6061 aatcactttt ccatccaata caaccactgc tccaatcgga acttcatgaa gcatttctgc
     6121 tttctttgct tctgccaatg caaggcgcat ccatttttct ttttctactg catcaagatt
     6181 tgtatttacc aattaccctt ccctgccttt gttttttctt gttttacttt agcatatgat
     6241 aaaaaggctg taactaactt ttttttctat ggtaaaataa atacaagagg tgaacaagat
     6301 gaaaatggat aaattacttg ctctttaccc acaagcacag aaaaaaaact ctccagcatc
     6361 agaagcggat atcctttctt ttgcaattga tgatgctttt atctggatca aacaagattc
     6421 attatctcaa caagaaacca gccttttgaa agcgttattc cctgtaatca acgatcaaaa
     6481 aaaacaccca tggtatcact atctttttaa agatgcttct tgtgttcaag aaaaaagctt
     6541 tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga
     6601 tactattttg gaattatttc gctattctgc tgatttcttt ttttatacag acacagatgc
     6661 gctcttagtc gaacaaataa accgtgatta tcttgatgct actgaaatca acggtatttt
     6721 tctatcttta gatgctgatt ttgatacgac aacgtctgta ttcgttggtt ctttccattc
     6781 acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt taacagagca
     6841 aaacaatctt tatactcaca gccgtacttt ttctatgcaa gatgtggctt tacattacta
     6901 tacaaaagat actgtaaaag atagtaagtt gatcaaaagt tacatagaac tgatcaaaca
     6961 aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc
     7021 tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga
     7081 acagtctggt ttcaatctaa aaaaagccaa cgatttactg ttttgctatc ttttattgct
     7141 tcatgaacat aattaaagat agattaaagg ggttgtgaca ataattttgt cacaacccct
     7201 tatttcgaat gaatagtgtt caaaagctag tttctcttac atcatttttt caacaaaatg
     7261 cattagccgg tcttttacat cttcggttga atctttgatt gaatcaccat aaacagagat
     7321 tttctttttt cctgatttga tttttgctaa cattcccatc aaagaatcca agtcactatc
     7381 tgaaagacca tctacgattt ctagtaattt ttcatttcca tcaaatttgt catagacgaa
     7441 tttttttact ttcgtccgag tagtcatatg atggatcttt tccattactt ttcccgatac
     7501 aacgactgct gtcgctacac tggcagcggc tgctacgctt aatccgattg ttacttttgt
     7561 tgatgttttc ataagtatca gctccttttt atctatctcc atcatagcat agacaaaaaa
     7621 ataaacgaac taatccactc agtgaaatct caatccttta gggaaaaagt tctttactgt
     7681 tccttttact tgtttcccaa atccgacagg tatcttatct aatactagca aaacccatcc
     7741 ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt ccttttgagt
     7801 gatcggcaag caaggtatat tctctatctt ttttgtagca agcgctaatg catagctagg
     7861 ttcaaatcga ttctttttga aatctcctaa atgcaaacct gtacgtacga cttttaaacc
     7921 atcgagagaa ggggctaact caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct
     7981 tccagtagct tcataatgaa aatcatttga aaactccgtc cagagttttt cctgctcttt
     8041 tgtcatttgc actttacttt tttttcgtgt cttcttttct ttatgcatct gattttgtcc
     8101 atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga tctttatgag gccatatacg
     8161 tattgttttt tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac taacggattg
     8221 agtcaaagga atctcctcaa tcgttactgg ataattttct acaagccagc taatgatctc
     8281 ttcattttct tctggtgcaa acgtacaagt cgaataaatc agttgtcctt tattttttag
     8341 catttttatt gctgaagaca aaatctcttg ctgacgtttt tgacagtata gcggagattc
     8401 ttctgtccac tcttttattg catttggatc ttttcggaac attccttctc ctgaacaagg
     8461 agcatcaacc acgatccgat caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc
     8521 atgatttgtc actattgcgt tcgaaactcc ccatcgttct atattttctg ataaaatctt
     8581 cgcacgtttc ggaaagatct cattcgtcac taataaccct ttccccttca tttgagcagc
     8641 taattgggtt gatttaccac cgggagccgc acacaaatcc aaaacttttt caccgggttt
     8701 tgctgcagca gctgttccaa cgatcattgc acttggttct tggctatatt catacccagc
     8761 ttgatggagg aaagactttc cattgacagt gccaagaaat ccctcattgc tgtagggagc
     8821 gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg
     8881 attccaccga aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc
     8941 ttcttcgcct aaaagaagac gatatttttt gataaattgc tgtggtaacg ttgtcgcttc
     9001 ctccttcatt ccaatgtttc gttagacatt ttacaatctt ttttacgaaa aataaaggtg
     9061 gctgatctac tttttcatgt tgctgcctaa ggtgactgtt cctttaagat attttccaag
     9121 tattcatccg tcacctcgtt tttatcccaa acagcgttca tcatttcaag aaaatcttga
     9181 gaagaatcat ctgagcgcaa acttcccaat gcattaagcc ataccaattc aagcgacttt
     9241 tcttctttga tcttcgtgtg acctttgaca tatgagaaaa atggccgctt aaatagcgaa
     9301 tccatgtcta aatcagattt tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa
     9361 tctttcaata agtagacaat gcttaaaaca tcatcggaac tgcattcagc taagtagttt
     9421 tcaaaataaa cagtgatttc ttctaatatc ttctctttta acccgctgat tgatttgaaa
     9481 tgtgtataaa tcgggtatgt agaacaaccg cagaactctg ctaactctct tgcagtaatt
     9541 gacttcattc catattcttc cacgtatacc atacttttga acagcaaata ttctttacta
     9601 taatttgtta ctctccccat cattcttacc tccgtctctt t
//
LOCUS       DACDHJ010000090         9494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDHJ010000090 DACDHJ010000000
VERSION     DACDHJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.092"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..797
                     /locus_tag="IV223_001632"
     CDS             21..797
                     /locus_tag="IV223_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8711923.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            812..1015
                     /locus_tag="IV223_001633"
     CDS             812..1015
                     /locus_tag="IV223_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8711924.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1031..1369
                     /locus_tag="IV223_001634"
     CDS             1031..1369
                     /locus_tag="IV223_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8711925.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1356..1535
                     /locus_tag="IV223_001635"
     CDS             1356..1535
                     /locus_tag="IV223_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711926.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1578..2048)
                     /locus_tag="IV223_001636"
     CDS             complement(1578..2048)
                     /locus_tag="IV223_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711927.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2135..2833
                     /locus_tag="IV223_001637"
     CDS             2135..2833
                     /locus_tag="IV223_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8711928.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3011..3352
                     /locus_tag="IV223_001638"
     CDS             3011..3352
                     /locus_tag="IV223_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711929.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            3345..4016
                     /locus_tag="IV223_001639"
     CDS             3345..4016
                     /locus_tag="IV223_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8711930.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4022..4708
                     /locus_tag="IV223_001640"
     CDS             4022..4708
                     /locus_tag="IV223_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711931.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            4711..5460
                     /locus_tag="IV223_001641"
     CDS             4711..5460
                     /locus_tag="IV223_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8711932.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            5472..5741
                     /locus_tag="IV223_001642"
     CDS             5472..5741
                     /locus_tag="IV223_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711933.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            5903..6205
                     /locus_tag="IV223_001643"
     CDS             5903..6205
                     /locus_tag="IV223_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711934.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            6202..6363
                     /locus_tag="IV223_001644"
     CDS             6202..6363
                     /locus_tag="IV223_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8711935.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            6360..6665
                     /locus_tag="IV223_001645"
     CDS             6360..6665
                     /locus_tag="IV223_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711936.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            6665..7021
                     /locus_tag="IV223_001646"
     CDS             6665..7021
                     /locus_tag="IV223_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8711937.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            6981..7226
                     /locus_tag="IV223_001647"
     CDS             6981..7226
                     /locus_tag="IV223_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711938.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            7223..7642
                     /locus_tag="IV223_001648"
     CDS             7223..7642
                     /locus_tag="IV223_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8711939.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            7639..8196
                     /locus_tag="IV223_001649"
     CDS             7639..8196
                     /locus_tag="IV223_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8711940.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            8193..8489
                     /locus_tag="IV223_001650"
     CDS             8193..8489
                     /locus_tag="IV223_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711941.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            8566..8979
                     /locus_tag="IV223_001651"
     CDS             8566..8979
                     /locus_tag="IV223_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8711942.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT         3524 a         1458 c         2033 g         2479 t
ORIGIN      
        1 aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa caaaacgaag
       61 ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt gcgagtgttt
      121 tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa gataagatgg
      181 ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac gaatctggtt
      241 tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa
      301 caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca gaaaaagtag
      361 aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta aaagaagaaa
      421 gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa atctcctatt
      481 tagatagcat attatcttct acagattcag taacaattag tcagattgca gcagattatg
      541 ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag aaaaaagtcg
      601 gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa tctcatacaa
      661 ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa tggacacaga
      721 aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct caaatggatt
      781 tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa tcactcaacg
      841 agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt acaccatgct
      901 tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg gtaaaaagac
      961 ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat tgtaaaggga
     1021 ggaacagcta atgcaatatc tagaagcaaa aatcccaatt ccagaaggct atgtaattat
     1081 ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat ggatgacgtt
     1141 gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta gagttttaaa
     1201 gaacccaaga tatagaaata ttatcgatat cgagaaaaac aaaaatggat ttgtctatta
     1261 tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg aatttttaga
     1321 aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat agagcgctag
     1381 tgttcagcgc accgctaatc atctacgctt taggactttg gggaagcagg caagcgttga
     1441 taggaacgat tgtttacatg gtttggattt ttatggggct tgatgaagct gaagctgagt
     1501 acagagcgaa aaagccaacc gagggggctg actaaaaatg aatagaaaag agaaactaga
     1561 atgtatatta ctattactca gtttaattct ttcactaatt tctctattgg gaagtttgta
     1621 tttttgatat caacaatttt gtggatttgg gttctattta gagtaaatat catttttgtt
     1681 tggtgattaa ggagtttatt aaaattaatt ttccctactt caaatgcaag gtaacaagat
     1741 atagcacttt taggaggaat atttagcggt acagcttcag agtatattct attatcatcg
     1801 actgatcctc ctttagcaat taatttctta aatggagtag ctgaccatct gcggtttaag
     1861 ttagcggatg aaagttctaa attaactaac atagctggtt ctgttgaata gttagaaatg
     1921 attacttttg tataaatcat atctgcagcg aagtaagaag cattaagttc aacttctagt
     1981 tgagggcgca ttccttttat ttttatccct gttaaaaacg tactcaatat aaaacttatt
     2041 attgacatcc attgaaaaat tgttaagtga aaaaaattca aaataaacac caccagtttt
     2101 taactaaatt ataccaaaaa ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa
     2161 taaagaagct gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa aacatcaaca
     2221 tgttttaaga gatattgatg cactaaaaaa agatgtgtcc aattttggac agatgtttgt
     2281 agaaggtaat gaaccagatt catatggcag aaatcgacga gttttcttca ttagtagaga
     2341 tggatttttc ttgctggcta tggggtttac aggaaagaaa gctatctact tcaaacaaaa
     2401 atacattgaa gcattcaacg aaatggaaga tgttattcgc aagaatactg ttcctcaaac
     2461 aattgaagat atgatgatct atcaattaga agaaatgaaa gatgttaaaa aagatgtttc
     2521 catgcttaaa gatactatgc gaattagcgg acaacaagag tttgaaatta agcaaaaagg
     2581 aaatatgaaa gttatgggag ttctaggggg aaaagaaagc cgagcttatg aagaaatcag
     2641 caaaaaagta ttctcaaaat tttggtctga atttaaacgt accttttcaa tcccaagata
     2701 tggcgagtta cctcgtaaga gattcgatga tgctgtttca tttattgaaa tgtggttgcc
     2761 agaaactgcg atccgtatgg aaattgatca actgaacaga caacagagac tttttggtga
     2821 tgacaatgaa tagagctgaa gcgctaagaa tagggacggc aattgctaat tgctggtgga
     2881 aatactataa accaatcatc ctaagccaac aacatattga caagcaaaaa gcatggcaac
     2941 aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa agaaaacaca tcataaggag
     3001 attttagcat atggaaaaag aactttccac tctagatcaa tatttgattg atcctgattg
     3061 gggcaagccg aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc ttttgacgaa
     3121 tgaagaacta gcttgggatc aagatgattt aggcaaccat gtaactattt gggatcatgt
     3181 ttatcttatc catctatcga agcattcgaa taaacctgaa tatatttacg tcatcgaaga
     3241 tggcttgatt gatgcgctag aagagtacga cagagataac ttgattgata tctcttatta
     3301 cggaccaggt aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag
     3361 ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag
     3421 gaaattttga ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac
     3481 aggcaacggt aactattaat gagtacaaac actacagggt tgtttctgga actcatcaag
     3541 actttcttgt tgaggaatat aaaccttttc ttatggatga aactgggact tatgtatctg
     3601 tctcagtaac ggttaaagga cacacggaaa ccgaattatt tcctgtttta gattatcgaa
     3661 accaaccagt tgttaaacca aatgcaatgc aaatcaataa ctcattgaag cgatgctttg
     3721 tgaaagcatt ggctctacac ggactgggat tatatgtatt tcaaggggaa gatattccaa
     3781 caccacctag aatcgataca aagaaattaa acatgctaga gacgattcta gaagctttca
     3841 atgagcagat gggtaaagat atgaccaaaa ccttaatcga atatgttaat gagcagacag
     3901 ataaattagg gctcttagct gataacgttg aaactattga acagttaagc tatgagcaat
     3961 gtgccttgat ggagcgagca atagcagcta agagaaaaga attagataag aagtgatatg
     4021 agtgtttaaa ccattaatcg attcatattc agcggttctg aaaaagttca aaggaaaaga
     4081 cataagcgca accatcaatg aggaagtgaa cattgatcga ctaaagacga tgtatgacgg
     4141 ctacgatggt gatcgagtca ttgaaattcg ttttattgat cctagacgtt tcaccgtaca
     4201 gcaacgaaac ttcatctatg cgctgatagg cgatattttt atcgatacag gcatgccaac
     4261 ggacttctgg aaggaattct tctactttcg ttttgaaggt gtcacagggc gcaaaataag
     4321 cctgaaagac gaatcgaata caactgtgag tgatgccaat gtcttagcaa atatcatctt
     4381 agatttcatc tttgaacatc atattccttt caaagaaggc tatgagattt tacctgcgaa
     4441 tcaagaatat tacttctaca aatgcatcac aaaaagagtc tgctgcatct gtggcaaaac
     4501 aggagctgac atcgatcact ttgacaaagc gctaggaaga cgaaagcgca aagaagttga
     4561 tcatgcagag tacacatttg cagcactctg cagaatccat cacacagaga agcacaaaat
     4621 aggtgtgatt aatttcaaaa ataaatatca aatcaaaggg atcaaattaa accagaaaac
     4681 aatcaaaaag ttaaatattg gagggtaaaa atgacagaac atcgaagtta ttatgcgatt
     4741 ataccagcca acgtaaggta cgacaaaaga cttaaaccaa atactaagtt gttatacgga
     4801 gagataacgg ccttgtgtaa tgaaagaggc ttttgttggg caggcaatga gtactttgca
     4861 gatttatatg gtgtgaataa agagaccata tcgcgatggg taagtgattt gattaagttt
     4921 ggatacttga atcgggaaat catttacaaa gagggtacca atcaaataat caataggtac
     4981 ctacgaatta atcaataccc tattgacgaa aaacgcaata cccctattga cgaaaaagtc
     5041 aaagataata atacatctat taataataca tttaataata caaaagaata tataagagag
     5101 ttaccgcctt cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg agagtataaa
     5161 aatgttcttt tgtcagatga gcagatggag aaactcaaaa cagaattccc taatgattac
     5221 caagagcgaa tcgaacgact gtcagagtat tgtgaatcat ctggtaagac ttataaaaac
     5281 tatttggcaa ctattcgaag ttgggcaagg aaagaaaaaa gtgaacctaa gaacgcaagc
     5341 agtggataca agcgcacagg aagacgagag aagcttcctg aatgggcaat cgaccaagaa
     5401 gcctatctca agaaaaaagc gctagaacga gctaatagac aatcaaaagc accattctaa
     5461 gaggtggaaa aatgaagatc gattatctag aactaattaa tgaaatagcg aattataaaa
     5521 agggcgagga attagacgtc ctgagagacg tatatgatca actcgaagaa gctggaattg
     5581 aaggaattaa gaatgatcgt tcgagttgga gtaagctcag atactatttc gcactttata
     5641 tcgatacaac acaattaaga aatttagcat ataccaaatt actatttgtt gattgtgtta
     5701 aaggcttgca aaaacatctt aatgaacttg agcaggtgta atcagatgga cctaaagaca
     5761 tttacagcac agattgaact aatgcatcaa gaagctttaa gacaaagtgt atcgtacgaa
     5821 gacaagtggc tcaacacgtt ccatggcgga cgtgagagcg cacttgatca agtactcaaa
     5881 ttactgaaag gagaatgtcg ggatggataa gaaagcggca atgcagcgaa ttatcgaatt
     5941 gacttattca gaagattggc aaaatgacaa agaagctgct tcagaagtga tgaggcttgg
     6001 aagagcgatg tgggcagaca agagcaacaa gccaagacca cgaaaaatcg caatttggca
     6061 cggtgacaaa cttctagtga tagggacagc tgaacagtta gcaagtctca caggcttgca
     6121 cgagaaaatc gtgaggaaaa gagcaaggtg tggctacaca gacgctaaga agagaacgtt
     6181 tagatacgtg gaggaatcat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc
     6241 cttcagcgtg agattgacga tctggaacga acaaaggcag tgatggtcaa tgaaacggcg
     6301 agaaaggcaa tcgatttgca catagagaat ttaagaaggg aaatccatcg attggaggaa
     6361 tgagcgtgga taagaaagca gcaatgaaaa gaattgctga attaaccaag tcagaatctt
     6421 ggcaagaaga caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
     6481 aaaagcccaa acggagaacg ccgagaaaaa ttgcaatctg gcatgatgac cgaattttag
     6541 taacaggtac tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg
     6601 atagagctag gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga
     6661 gataatggat ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat
     6721 tcagaaagat aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac
     6781 aggagcagga gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta
     6841 caacgaaatt caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag
     6901 caagaaactt catcaagatc gtttcaaatt tgtagagaag tataaagaaa tattagaaga
     6961 gtatttgagg aggacagcaa atgataaaaa aactcgttca attcagcatg gatttatatg
     7021 atatcgaatc aggagcaaca ctatctgtgg aatcggacca tctaatcata aattttggtg
     7081 gaaagcgcca gattattttg tgggtagttg atgatgtact gtttccagaa attgttcatg
     7141 atttcgaaga atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg
     7201 aaaaatacga ggaggacagc gaatgatacc gaagtttaga gcgtgggata aacgaaagaa
     7261 cgtaatgaga gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa actttattga
     7321 atattggata aatcctaccg aattgaaatc atatcacgtg cgaaacatcg acctcatgca
     7381 atccacagga atgaaagata agaatggtgt ggatatattt gaaggggata tagtattagt
     7441 cagcgtgcga aatggcttcg attacttaga taataaagtc tgtattgtca aaaattcaat
     7501 agattattcc ggattagttt gtgccactgt tgatgaagac ttagagtatc aaatttttaa
     7561 cacagagctg tttgaagaat acacgtacga agtcaccgga aatatatacg agaatagcga
     7621 gttattggag gaacagcgat gaataaacag gaagcaatca aaaagttaga aagtattaaa
     7681 gcgataggaa atgatgcaat agctgcttgc tataacgaga gtataaattc aggtattacg
     7741 ttaatgaaaa aaatagacga accgcagaaa ccagttattc cacaactcgt ggccggttgg
     7801 cttgagaaat ctacggaccc ttttacaaaa gctgaaaaaa tagcgtattt aatcaaatct
     7861 aaagatggtg attcatatta tttctgtgat tggtttgtac gagatggcat agtgacgcaa
     7921 gagcaaggag aagaattact cgcttgggca acaagacaat catatgaaac actattgagc
     7981 ctatacaacg gctacgaggt tgagaaagag ccgttatggg caataaagaa tgccgatgga
     8041 aactatctta ctaaatgtgc tttatgggga aaagatggag taaattatag ttttgaatgc
     8101 aatccatctc atcgattgct tttcactgat aaagcaacag cggatgctgc agcattgttg
     8161 gtgaatggaa cagtggaaga ggtggtagaa agatgaaact aaaagacgga ttttacgcta
     8221 gcagtcacgg tatcggcggt ttaatgctag atatgccgac aaagaaccct aaaacacgca
     8281 agaaatcaaa attcaaagtc ggtgacatgg tccgctgtga agcagaagag ttcgtttatc
     8341 cgttcagagg atacgttaaa aagatactgt caaactcagc aatcattcgt attgaaaata
     8401 caatgaagtg tgatcgttcg actgcaaaac acaagcatta tttagctgta gcgagattag
     8461 ttgacatgga agtaatcaag agcaaataaa aaaagccgga tcgctccgac tgattcaata
     8521 aatccaacac atttattata tcacataaag gagcggtttg acttgatgca attgttacga
     8581 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     8641 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     8701 ccgaaggcac cgaagcatgg caataaggca gaagacgcga ttattcagat gatggatata
     8761 gaaacggaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     8821 atactctact acagcttctg tgtgccagat agcttctcaa actacagaat tagccgtgaa
     8881 gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca
     8941 gaagcatata aacacggaaa aataattgct tataaataat ttggcggttt tttggcggaa
     9001 tgatggcggt ttttagacat ttaccagtgt tattatggta gtgtcgaaag attaggaaac
     9061 aggatcgaca aaataaacga agggaggcaa tctccctcat cgttttaaac ttcttcttag
     9121 atagacagca actaattatg aagaaaagga tgtgaattca actcctccta aattcttcat
     9181 cggttgctgt ttattttgat tagacagcag cactcacaaa actaaacatg ttcttgtcgt
     9241 ctttcaactg ctgctgtcta ttaatttatg tattggaggg aagaaaaatg aaattatcag
     9301 tagaaggcac tccagaagaa atacaagaat tgctccaagc tattggtagt agcaaggagc
     9361 aaaaggtaag tgtttctaat tcagatattg acggtatttg caattgttta aacagagaaa
     9421 aaatatctaa tccttactaa gagaaaatat gttcgttaat gaatttccaa ttttcctctg
     9481 tagctctacc ttga
//
LOCUS       DACDHJ010000091         9278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDHJ010000091 DACDHJ010000000
VERSION     DACDHJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.093"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            10..1434
                     /locus_tag="IV223_001652"
     CDS             10..1434
                     /locus_tag="IV223_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8711943.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1733..2440
                     /locus_tag="IV223_001653"
     CDS             1733..2440
                     /locus_tag="IV223_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8711944.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2427..2750
                     /locus_tag="IV223_001654"
     CDS             2427..2750
                     /locus_tag="IV223_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8711945.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2839..3876)
                     /locus_tag="IV223_001655"
     CDS             complement(2839..3876)
                     /locus_tag="IV223_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8711946.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4197..5180)
                     /locus_tag="IV223_001656"
     CDS             complement(4197..5180)
                     /locus_tag="IV223_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8711947.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5595..6365)
                     /locus_tag="IV223_001657"
     CDS             complement(5595..6365)
                     /locus_tag="IV223_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711948.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6358..7293)
                     /locus_tag="IV223_001658"
     CDS             complement(6358..7293)
                     /locus_tag="IV223_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8711949.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7609..9258)
                     /locus_tag="IV223_001659"
     CDS             complement(7609..9258)
                     /locus_tag="IV223_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8711950.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
BASE COUNT         2717 a         1946 c         1694 g         2921 t
ORIGIN      
        1 aaaaaaatta tgacaactat gaaaaagcta acttttccag atgatttttg gtggggttcc
       61 gcttggtcag ctgagcaagc agaaggaaaa ggagaaacag gaaaagcaga gactatctgg
      121 gatcgctggt ttaaagaaca accttaccgg ttttatcaac gaatcgattc ctctattaca
      181 accgatcaca tccatcaata taaagaagat atagaactaa tgaaaaaaac tgggcataac
      241 tcctttcgcg tttctatttc ttgggcacga atgtttccaa atggcggtac cggaaaagta
      301 aatcccaaag ccatttcttt ctatcgaaaa ttattttccg aaatgaataa taacggaatc
      361 aaagtatttg ccaacttata tcattttgac atgcctgcta ctcttcaaga taaaggagga
      421 tgggaatcac gtgaagtagt tgatgcttat gttcattacg ctgaaacttg ttttaaagaa
      481 tttggtgact tagtttgtca ttggtttacc ttcaatgaac cacttgggcc tattttaggt
      541 acttatcttg aagattttca ttatccaaac ctcattgact ttaaacgcgg agcgcaagca
      601 gcattcttca ctatacttgc acacgcaaaa gcaatacaag tatttaaaga actagctctt
      661 cctagtaaaa taggcgttat tctaaatttg agtcctactt ttcctcgtag tcaaaatcct
      721 gctgatgtga aagcagcaga aatcgctgat ttattttata cgcggagctt tttagatcct
      781 atggttaaag ggaatttttc taaaaagctt atcctgttat tacaagaata taatcagctt
      841 cctgaagatt atacgaaaga agatcttgta tgtatctacg aaaataccgc tcaaattctt
      901 ggactaaact attacgagcc acgtcgcgtc aaatctcgtt cttctatggt ggatacatct
      961 agtccatttt taccagagtg gtttttcgaa ccacatatca tgcctggacg acgaatgaac
     1021 gaataccggg gatgggaaat ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag
     1081 aaccattatg gaaacatcga agcatttatc tcggagaacg gaatgggcgt tgcggatgaa
     1141 gaacgtttca tggatgatac agggcaaata attgatgatt atcggattga gtatatcaaa
     1201 gatcatcttg cttacttatg gaaggcaata aatgaaggat gtaacattaa ggggtatcat
     1261 ctttggacat ttattgattg ctggtcatgg atcaacgcct ataaaaaccg atatgggctg
     1321 gtttcactcg accttactac tcaaaaaaga acaattaaaa aaagtggcga attttacaag
     1381 caattaagtg atacaaatgg atttagatac gatgtagact tgttagttga ataatattct
     1441 aaaattccac ttgaagtgaa cacattagaa aacaagaata atgagaaata ccaataacaa
     1501 atactttttc ttcatacttc atttatactt gtcagtataa gagactgtga caaatgtagt
     1561 gatcctcaga agttaggtct ggaaatctaa cttgagaaga tttttttaaa ctacgtccag
     1621 tcttttttat ttgtttaaaa ttgaagttga caagtcttca ttaaaaattt agaatattca
     1681 caagtttgac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt tattggatga
     1741 aaagttagat ataaaaactg cgatcaaaga cacgttacct accgtttttg gttatatcgg
     1801 tattggactt gcatttggta tcgttgggaa agctgccggg tttcatccgc tggtcgtcac
     1861 gttgatgtcc ctgctgatct atgctggttc tgcccaattt atcacggtca gcatgcttgc
     1921 tagccatagt ccactgctct ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat
     1981 tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc aaaatctgct
     2041 gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat tgaactatac
     2101 tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt atttgacgtg
     2161 ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc cgaaaaagtt
     2221 tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc aagtcatttc
     2281 tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt tgattctggt
     2341 atatgtcgga ctgattttta tcccaagtaa cttagtgatt ttagttgtaa cactgattgg
     2401 gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg ctgactattt
     2461 taggttgtac tttagcaact tggctttcta ggatcctacc atttgtctta ctgaaaaaat
     2521 tcgatttgcc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc attatgtctg
     2581 ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag atcaatctcg
     2641 aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa agcttattgg
     2701 tgatcgtctt ggtaggaatt ctttcccttt cgttgattcg cttattataa ataaaaaaac
     2761 aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa
     2821 aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt ttcctaatat
     2881 atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca tcccatctga
     2941 atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag caatgtgcgc
     3001 taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga acatatactc
     3061 ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct ctttgatccc
     3121 tacgatcgtt cccacatgtc caaatggtcg gacaaggtgt ttgatttgat caaagtagcg
     3181 ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt cacgaaaact
     3241 tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt tcagccattc
     3301 aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt
     3361 caaaatcgaa ccgacgcccc cagctccgtt gatcactaag atttttttcc cgcggttggc
     3421 attttcttct ggagtcagac caaatttttc aaataacaat tcataagcgg tcaatgaggt
     3481 caacggcaag gctgcggctt cttcatctga aagatttttt ggtgcaagag cgacaatacg
     3541 ttcgtcaacc agttggtatt cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa
     3601 aacgcgatcg ccgacagaaa atttttgcac ttcatcacct acagcaacaa ctttcccgac
     3661 tccatcaaac cctaagatac gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt
     3721 atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg aactgggtac
     3781 aggtatctgt tctttttcat caataaagct ttttggatca tcgatcgaca acccttcata
     3841 aaaaccgact accttcatta attccttttc tgacatacga attcctcctt agcttcatca
     3901 agttggagca tccatacagt tttcatttca atatatatag tatgctattt gttccacttt
     3961 tattcatcat aattattctc ttacttaaag taaactattt gcactttttt ggttaaaaaa
     4021 gaggttgcga caagactttt gtcacaacct cttttaaacg gtgttcaatc aactgaccct
     4081 cggtattcgc tcaaaaaacg acaaaatatg aggagctatt tttgtttggc tgttcttatt
     4141 acttgatgca ggacagcttt ttcacgacct ctaattcacg gtgttcaagc aagaaatcat
     4201 tgttcactga aaagcgttgg atgttctttt tcaaaggtgt ctaagatctc tcgtacttca
     4261 tcagtagatt tcgtattcat caactgaact cgcaagtcat ttgctcctgg aaatcctttg
     4321 atatagattt taaagaaacg atgtagtcct gtgatggaac gcgggatgat ttctgaataa
     4381 tgatcttgca gatctagttg cagacgaagt agtccgatca gttctttttc tgtatgttcc
     4441 ctcggttctt tttcaaatgc ataaggattc ttgaaaatcc cccggccaat catgatgcca
     4501 tctacgccat atttttcggc caattcttct cctgtctggc gatctgggat atctccgttg
     4561 atcgtgatca gtgtttgcgg agcgatttgg tcacgtaaag caatgatttt agggatgact
     4621 tcccaatgtg catctacttt actcatctct ttacgcgtac gcaaatggac agaaagattg
     4681 gctatatctt gatgaagtag gtgactgatc cattcttcca tctcagacat ttccgtatag
     4741 ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt tagccgcttc aatcaattgt
     4801 gccgctactt ctgggcgaag gatcaagcca ctgccttttc cacgttctgc tacattaggg
     4861 actgggcagc ccatgttgat atcgattcct tggaagccca tttctgccat agcaatgctc
     4921 atttctctaa aaaattctgg cttgtctccc caaatatggg caaccatcgg ctgttcatct
     4981 tctgtaaagg tcaatcttcc tcgtacactg tcttttccat ctggatgaca gaaactgtct
     5041 gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg catgctttac gacatggcga
     5101 aatacaacat ctgttacatc ttccattggt gctaaaacaa aaaaaggctt tggcagctct
     5161 ttccaaaaat tagtagtcat ttatttgtta ctctccattc tatcaagcag tgaagtcgca
     5221 atctttctca aaaacattgt cattatacta ggaaacaaaa aaagaagcaa agtgaacagc
     5281 ttaaaagatg tttctgattg agcaaaaaaa gactgtgaca aaaatcttat cacaattcct
     5341 tttccggaat aaatggtgtt caaaagcttg catctgcggt aataagtcca accaaacgaa
     5401 aaataggagg agctattttt gtttggctgt tcttattact tgatgcagaa cagctttttc
     5461 acaacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct caacaaacga
     5521 caaaatatga ggaactattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt
     5581 tcacaacctc ttttctaact tctcaatcct cttcctttat tgatcaaata ccaagtaacg
     5641 gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg atgttgctac tggcacatcg
     5701 attgttccaa ggaatccgta acggaaaccg gagatcatat aaacgatcgg attgactttc
     5761 gaaacagctt gccaaaatgg cggcaacata gagatggcat agaaaacgcc acctagataa
     5821 gttaacggct gcaagacaaa tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa
     5881 atcccgttga gcagtcctgc taacgaaaac aagattgccg tcatcaataa tgtaatgatg
     5941 accatgaacc atgaatacac atgaagtggt acaaagaaca aggaaatgat cgttacgaga
     6001 gagccgacga gaatactgcg acccactccg ccaatcacaa agccccaaat gatcacatgc
     6061 gtaggaacag gcgctaccaa gatttcttcg atgtttttct ggaatttttg agagaagaag
     6121 gacgaagaaa cgttggcata cgaactagtg atagcagaca tcataatcaa accaggcacg
     6181 atgaattcca tatatgaaaa accacccatg tcaccgatcc gaccaccgat catttttccg
     6241 aaaataacaa agtacaagga tgtcgtgatc acaggcggaa ccagtgtttg gacccagata
     6301 cgcaaatagc ggttcgtttc cttgatagct aaacttttta atgcagtaaa ataaagatta
     6361 aacatttttc tcctccgtat gattattttc ttctgtgatt ttcaaaaaca gttcttctaa
     6421 acgattcgac ttattcctca tagaaagaac tttgattcct tgtcttgtca gctgatcgaa
     6481 gattccgttc accccttgat ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa
     6541 tgtacaagcg tatccttcaa tcacaggttt ggtctcatat ggcgctaaat caaagataaa
     6601 tgtttcaaat tgcagtttgg atagaagctc tttcatacta gtattctcga tcagttcacc
     6661 tgattggatg atcccgatgt tgcgacaaag catttcagct tcttctaaat aatgcgtcgt
     6721 caaaatgatc gttgtaccat tttcgttcaa ttctcggaga aactcccaca tctcgcgtct
     6781 caattcaata tcgactcctg ctgttggttc atccaagatc agcaaacgtg gttcatgcat
     6841 caatgcccta gcaatcatca gtcttcgctt cattcccccg gaaagcatac gtgcacggac
     6901 atttctcttt tcccaaagat tggactgctt caaatatttt tcgctgcgtt tcatcgcttc
     6961 tttgcgagag acaccgtagt agcctgcttg attcacgacg atttgctgga ccgtttcaaa
     7021 gggattgaaa ttgaactctt gcgggactaa tccaatctgt tgctttgctc ggactagatc
     7081 cgtatccaga tcataaccga agacattcac ttttccagat gttttattga ctaaagaagt
     7141 aatgattccg atcgttgtag acttcccagc tccatttggt cctaatagcg catagaaatc
     7201 tccttctttc acggaaagat cgattcctcg caatgcttcg accccacttc cataaacctt
     7261 tttcaaatta tttatttcaa gcgcatttgc catttatttc ctatctcctt atagacatga
     7321 ttctttattg tatcatggaa tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc
     7381 aggttgctgg gctcaggaaa atctcctaat gaaagacatc aatcggacga agtcagacaa
     7441 ccttttcaga aaaaacaaat cgaagataaa ggattactat tcatatgtgc aacaaaagga
     7501 gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     7561 agcaaaagcc aagatttttg atccaataaa tactttcgat cacttcgcct atcccaatac
     7621 atgccactgc cgcttttcat caaataacgc ggcttccgtt tttgcggttt ctactaaagt
     7681 ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt cgggtccttg aaggtccttc
     7741 ttttaatttg aaatcaaaac tgattccttt tccttctgtt acttgttctt caaagtggac
     7801 attctcacaa taattcttta aaatctctgt caattcgata tcatgtgtgg cgacaaatgc
     7861 taaagatgga tactccgcaa gccaccgaac gactgaagcg gaagaagcaa tccgttcgat
     7921 cgtatttgtc ccttttaaaa tctcatctac gaagcagtag cagcgttctt tggaagccac
     7981 ttggtccaac aaacgtttga tcgatttgat ttctgaaaca aaataactgt cgccttcaaa
     8041 taaatcatct tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg taaactgttc
     8101 tgcaatcgct gtttggatcg tctgtgctag aatacagctg atggctatac ttttgacata
     8161 cgtagatttt cctgatgcat tggaacccgt gactaatgta ttcttctgcc agttgaccgg
     8221 attcactacc gcttctttta acaatggatg atagatatct ttcgctgtca caccgccctc
     8281 agtaaactct ggtcggcaag tgatgggcat ataagtacgg taattcaata ttgcagcggc
     8341 tacttctaat tctcccagca attcccacag cttgatggct tcagttgaat gcttttctaa
     8401 tttagtgaga acgaaatgat aggagataaa tggcagcatg aacatgatgt tgacatattc
     8461 aaaaatcaac tctgcttcac tcccactctt catacgaaat gaaaaaccta atttagggat
     8521 gtctcttaat ggtttagttt gctgtttcag ctcttcttga agtggtgtcg gcagttttgc
     8581 tagctgattg gcggctgaaa tcgttgaaac caaataactc atgctattca gctctgtttc
     8641 tagtgtttgt ttcttcacta gataatagac cgtattgaat actaacccac cgattgccaa
     8701 atagattcct agtacttgtc ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc
     8761 aagaaaaata aataaaccta cgcgtccaat ctttttactt tttccatccg ctagatattg
     8821 ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat tgatattgga ttttttctct
     8881 cagttgagga ttttcctgat aaaaagcaat cagtttttct aactgctgat cttcaccaaa
     8941 atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct acactagaat aggtactgtt
     9001 gattccttcg aatacttcga acatatccag gtcataccaa gtaagatcat cgatttcgct
     9061 atcccatgaa cggaacttct tctccgtgag ccatgcttcc ttcaagcttt cttctttatc
     9121 aaatctcggt tgataaggaa ttttttccca tttgctacga actgtttcct ttaatttcaa
     9181 acgtgaaaaa atgtctaaag caactaataa aataacagct gcaataataa ttaaaacgat
     9241 aaattctctt tgacccatat ccctctccgt tcctttat
//
LOCUS       DACDHJ010000092         9233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDHJ010000092 DACDHJ010000000
VERSION     DACDHJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.094"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            149..1051
                     /gene="gnd"
                     /locus_tag="IV223_001660"
     CDS             149..1051
                     /gene="gnd"
                     /locus_tag="IV223_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8711951.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            1053..2054
                     /locus_tag="IV223_001661"
     CDS             1053..2054
                     /locus_tag="IV223_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8711952.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            2051..2335
                     /locus_tag="IV223_001662"
     CDS             2051..2335
                     /locus_tag="IV223_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8711953.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2368..3165
                     /locus_tag="IV223_001663"
     CDS             2368..3165
                     /locus_tag="IV223_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8711954.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3179..4012
                     /locus_tag="IV223_001664"
     CDS             3179..4012
                     /locus_tag="IV223_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8711955.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            4030..4887
                     /locus_tag="IV223_001665"
     CDS             4030..4887
                     /locus_tag="IV223_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8711956.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4909..5418
                     /locus_tag="IV223_001666"
     CDS             4909..5418
                     /locus_tag="IV223_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8711957.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5455..5856
                     /locus_tag="IV223_001667"
     CDS             5455..5856
                     /locus_tag="IV223_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711958.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5968..6321
                     /locus_tag="IV223_001668"
     CDS             5968..6321
                     /locus_tag="IV223_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711959.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6355..6798
                     /locus_tag="IV223_001669"
     CDS             6355..6798
                     /locus_tag="IV223_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8711960.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6815..7900
                     /gene="uxuA"
                     /locus_tag="IV223_001670"
     CDS             6815..7900
                     /gene="uxuA"
                     /locus_tag="IV223_001670"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8711961.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7893..8606
                     /locus_tag="IV223_001671"
     CDS             7893..8606
                     /locus_tag="IV223_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8711962.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3105 a         1448 c         2052 g         2628 t
ORIGIN      
        1 tcataataag attatcattt tttccgttct cattaatgtt aaagatacaa ggagagtagg
       61 aaaaaatcaa acaaaaaata tttttgtaaa atctatttac aaaaaaattt ttttgtataa
      121 tgagggtata agaaatggag gcgctatcat ggaaattggt gtaattggtt tgggaaaaat
      181 gggattgaat ttagctttaa acttaaaaga gcaccatcac aaagtacaag gactcgacat
      241 ttcaagtaca gctgttagtc aggcaagaga aaatgacatt cctgcttatc aagagatctc
      301 tgaatttcta gctacttttg agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga
      361 gataaccgaa aacatgttaa gaaaactttt tccaaaactc ggaccaggag acattattat
      421 tgaaggcgga aactcttatt ataaagattc aatccgtcga gcagaagagt ttcagaaaaa
      481 tgaaatcggt tacttggatt gtgggacttc tggaggaatc gaaggagcac gacatcaagc
      541 ctgcttaatg attggaggag ataaagaaac ttttctatcc gtggaacaat tattcaaaga
      601 tgtagcaatt gaaagaggat atctgtatgc tggtgcatcc ggaagcggac acttcttgaa
      661 aatgatccat aatggaatcg aatatggaat gatgcaagca atcggtgaag gattccaact
      721 tgtacaaaaa agtgagtatg cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg
      781 ttcggtgatt cgcggctggc tgatggaaat catggaagat cagttaaacg aacatcccaa
      841 tttaagtaat tatcggggaa tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac
      901 agcgctagaa atggctgttc cagttccagt aattgcaact agtttgttca tgaggaatct
      961 ttcacaggaa gaagatagtt tttcagcaaa agtagtttca gcactaagaa atgggtttgg
     1021 cggacatgaa atagttgaaa agggtgagta aaatgccaga ttatcatata gcaatcgtta
     1081 attccagcag tttcggaaga gtctttccac accacttgga aagattaaag tcagttggag
     1141 acgttcattt ctttactgtt gatcaagaga ttccaggaaa agaactagcg gagttactta
     1201 aaggattcaa tatcattatt gcaagtgtta cgccattttt tcgacgagat ttttttgaaa
     1261 acaaagatga actgctattg atttcccgac atggtattgg ttacaacaat attgatttaa
     1321 aagcagcaaa agaacaccag acaatcgtat ctatcatacc tgctttagtt gaacgaaatg
     1381 ctgtagcaga aaataatata actaatttgt tgtccatgct tcgacgcacg acacaagcac
     1441 aagcaagagt caaggacgat cgatgggaag atcgggcgga gttcgtcggt aggacgctgt
     1501 ttaacaaaac agttggtgtg attggtgcag gaaataccgg aagcggagta atcgaaattt
     1561 tgcgtaacgg ttttcgctgt gatgttctag cttatgatcc ttacaaatca gatttataca
     1621 tcgaaagctt tggtgcaaaa aaagtagaac ttcaagaatt actggaacaa tcagatatca
     1681 tttgtttatg tgcgaatttg acagaagaga actatcacat gatttctgat aaagaagtcg
     1741 atcagatgaa agaaaatgtt tatctatcta atagtgcaag aggcgctcta gttgatgaag
     1801 aagcgatgat taatggtctg aaaagcggaa agatcgcagg atatgccaca gatgttttag
     1861 aagaagaacc gggacgtcaa gatcatccgt atttagcgtt tgaacaagta gtgattacgc
     1921 cacatatatc ggcctacaca agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag
     1981 atgtcgaaca gatcagtcag caccttcttc ctgaacgttc tgtccagaca aagagcaact
     2041 atataaaagg atgatgaaga aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt
     2101 ttatttatat gaagaaggtg gtatttcaaa agtttctgat ctcttgaaat catacggcgc
     2161 gaaaagagta ctggtcaccc acggaacggt atcatgggaa aaggcgctac ctaaattagt
     2221 atttttgaat gacgaaacga ttcaattttt ttatcatcgt tattctggag aatgtagtta
     2281 tgctgaagcc agacgaatag ctacaatcat caaaaaaatg aaatcgattt cgtagttggc
     2341 gttggcggag gaaaactgac cgatctagtg ggttatagtg ccaatcttgc aagaatcgat
     2401 ttcggagtca tccctacttt agctagtaat tgtgctccat ggacacctct tgctgtcatg
     2461 tataaggaga atggacaagc agaagggaaa acagaacact tttacagaca agctgctttt
     2521 tttataacag atccccaatt agtgattgat tcccctgttc catatttcat tgccgggtta
     2581 gcagatacac tagcaaaatg gtatgaatct gagctcatct tagaacaaga ttttttgcaa
     2641 gatgaatcat ttttgaagct agcacaagat actgcgaaga tctgcaaaga agagattttg
     2701 aatcattctg ctaaagcaat ccaagatatg gcacaaagaa aactgacatc ggagttcaaa
     2761 cagctttcag agattatctt tgctgttgct ggtttagttg gaggattagg agataaatac
     2821 gcaagaaatg cggcagcaca tgctatgcat gacgctatca gtaaatatat tccggaaagc
     2881 catcgttttc tacatggaga aaaagtagcg tatgggattt tctatcaact ggcactagaa
     2941 ggtcgctggg aaaaaataga cgagttatat cctttgtatc gggaattaga actgccggtt
     3001 tctctagaac aaatgggatt atttccgaaa gataaacaga taatagataa aatggctgaa
     3061 tttattgatt cgaaagagaa agtgcatttg attcctatag aggtaacaca gaaatcactg
     3121 atacagtcaa tgatggaact ggaaggttat atcagaaaga tctgaaggag gaggacacat
     3181 ggaaacatta agtgtagtac aaagtttgtt gattgcttta tgggtagccg cggttatgtc
     3241 ccgctggtta ggtggaggag ccacgttgac cttacgtttt tctcctttga tgactggatt
     3301 agtcgtcggg ttgatcatgg gggaagttgc acaagcgatg attgttacag ccgctttgca
     3361 aatgatttat atgggggttt tttctccagg aggatcaatg ccagctgaac catctattgc
     3421 agcagctatc gcagtaccag tcgcattatt aggggatctg tcgccagaag cagcgattgc
     3481 cgttgcggtg ccagtcggat tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa
     3541 tactttttta ggtaaatata cagacaaagc tgtagaagat ttgaatacaa aagctatggc
     3601 ccgatcaatt attctttatc caacggttgc ctcattttta ttattcgttc cgcttgtttt
     3661 cattgcttta tatttagggg cgccattgat cgctgatgta atcaaagctt tagagggaac
     3721 agtcgttatc cacattcttg aagtagtagg tggagggtta gcggcaatcg gtatcgcgac
     3781 aactgtatac gttatcggtc gaaaagatta tctggtcttt ttcttccttg cttattttat
     3841 gagtgttgtt ttgaaatctt tagatattac gatggttact tatgccattt ttgggattat
     3901 tatcgctttg atctttgtcc agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa
     3961 taacaattca gcatcttttg atgacgaaga tgactatgat gacggatttt aatttaggag
     4021 gaatgggaaa tggctgaaat aatggataat caaacaggtg ctcctgaaga aatcacgaaa
     4081 aaagatgtaa caaaagccta tctgagatgg cattttgcaa acgaaattcc tcactctttt
     4141 gaacgttact tggcgccatc actactatac ggaatgatgc caatcttgaa aaaactttat
     4201 aaagatgaag actctttaaa agcagcatat aaacggcagt tactgttctt caacactcaa
     4261 ttaagctggg gtggcggtgt gattactggc ctaatgtctt ctatggaaca gcaaagagca
     4321 gaagaagagt atgaagaaaa agagatcttg atgcaggatg acttgatgta taacacgaaa
     4381 gcgggattga tgggggcact agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa
     4441 tatattttta tcgcaatcgc ggtaccttgg gcacaacagg gaagtccttt aggcgcactt
     4501 tttcctttta tctgctttgg gttgtatcag gttttactag gggtattttt cgcaagacaa
     4561 gctttcacta tgggccgtaa tgctacaggc ctaatgcaaa acactggtgt tcagacagcg
     4621 attgaaatgc tatcagtttt agggttgttt atgatggggg tgcttgcggg aaactatgta
     4681 aaagtagaat cttcattgaa atttgcgata tccggacgtg agtttgttat ccaggaaatt
     4741 cttgatcaaa tcgtgccagg gatattacct ttagctgtcg taatgggtgt gtacttgttc
     4801 tatacgaaaa aaggattgaa agtcacacaa gcattattat ggctgactgg aatattaatt
     4861 gtgctagcag gaatcggtat tttataaaaa gaagggaacg tgaataagat gggtgtagtc
     4921 aatttagcgc gtgtggatga gcgtttgatc catgggcaag taatggtaac gttatctcaa
     4981 aaaagtggag tgaattccat ttttgttgta gacgaagttg tagcaaaaga taaatttatg
     5041 agagatcttt acaaaaatgc aggaagccgt acaggacaaa aaacaattgt cattacacca
     5101 gagaaagcta aattctattg ggatgaatat caatttaaag aatacaattg tattttgatc
     5161 gcaaaaacag tccaaacgat ttacgactta gtaaaacacg gaatccccat ggaagaattg
     5221 aatattggag ggattgctca aaaaaatcct gatacagatc ttttagtgac gaaatctgtt
     5281 tatttaaata aatcagatgc tgaaaaattg aaagaaatga atgaacaata tggcgtaaag
     5341 aatatttatt ttcaagccac tccatctgca cctaaaacag gattaaaaga tgtattgaac
     5401 aagtttgatt tgtcataaac aaatgcttag aaaaaagata aagagggcga aaaaatgaag
     5461 aagaacgagc tgtttcgtga ttgggaattt cgttatcgct atatttaccg taagcgccgt
     5521 acaaaaaaga gtaaacagcg ttttctttca gcattagtat cagatattta cagtatgaga
     5581 acagatgtga cagtcattgc ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt
     5641 ggagatatcg aaaaagcaga gaaagttatc tgtacttatt acgatacacc cgttcatgca
     5701 ttaggaagtt atttcatgtt tgattggaaa gaccaaagga aaaaaacaat ctactctata
     5761 ttattgtcct tcattttgtt gttcagttta ggttggtggg gaatgatgat atataacaaa
     5821 aatcctcatc acgtattcga tctgctatcc gtataaacag gtatcactgt actagcattt
     5881 gggagctact ttttcttatt gggaaaagca gctcgcggat ggcctagcag acaaactttt
     5941 attcgaaata cttcatccat attaacgatg ctagaaatga tccgtacgat tgatgatccg
     6001 agtgtagcgt atgcgtttgt tgatgaaggc tgttatggag aaaagggctt agacagcgtg
     6061 agatcgggta tgaaaaaaga ggctattctt ttttatctag actctgttgg ggcagataca
     6121 ccgctacagt tttcgggaaa ttatttttca aataaagaac agtggctgaa acaagttgat
     6181 aagttgaaag aaaaaaacgt caattatatt tttagtgcaa gaaaaaagca agcgcagttc
     6241 ttctatttga ccaaaacaga tttacgaggg aaaacattta attggcaaaa tgccaatcag
     6301 attattgctc tttttcgata agtaaaaagt gcaaaacaag gagtgagaag actaatgttg
     6361 gaaatcatga tcgttacaca tggtaagttg agcgaaggac tgaaagatgc ggcggaagta
     6421 attgtcggga catcgagcca tattgaaaca atgtgcttag agtctgggga ggacatccaa
     6481 acattcggag agaaggtcaa agaaaaatta actatgatca acacagcaga acccatgcta
     6541 gtatttgttg atctaattag cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa
     6601 ctgactgaag aaaagaaagc agccacttat gtcatagggg gcgtgaatct gccaatgttg
     6661 ttagaagcaa tcaatcatca gatgcttcac acaccgatag aagaagcggt ggaacaaatt
     6721 gtcaaacaag gaaaagataa tttaactgtt tggcatgctt cagcggaaga aaaaaaatta
     6781 gatgaggacg atttttagaa ggaaggctga aaagatgaag tggggattta gatggtatgg
     6841 tgaaaaaggg gattcgatcc cattaaaata tgttcgccaa ataccgggaa taaatgggat
     6901 aattggaact ttgctgacta aaatgcctgg agatgtttgg gagatttcag aaattgagga
     6961 gttgaaagca tctgttgaga gccaaggact ggaactgctt ggaattgaaa gtgtagcgat
     7021 ccacgatgca atcaaggcag ggacagatga acgagattac tatattgata actatatacg
     7081 aacaatcaaa aacttgtctc gatgtggggt tcgattgatt tgttatagct tcaaaccaat
     7141 ttttggatgg gcgaaaactg atttgtttaa tgaaaacaac gatggaagct attctctttt
     7201 atacgatcag aaaatcgtcg atcagatgga gccaggagaa atgtatcaat tgatacacag
     7261 ccagtcaaag ggattcaaat tgccgggttg ggaagaagaa cggttgaaaa aattcgctca
     7321 gctggaaaaa ctgtatgaag gaatgaccga agaagaatta tttgaaaatc tgacttattt
     7381 tttagaaaaa gtgattccag tgtgcgaaaa agcagatgta aaaatggcta tccatccaga
     7441 tgatccgcca tgggaacttt ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa
     7501 gaaaattcta aatgcagtag attctccata taatggacta acgctgtgca caggatcttt
     7561 aggggcaaat ccggaaaacg atttagttga aatcatccat gaggtaggtt cgcgcatcca
     7621 ttttgtccac ttcagaaacg tggagttttt aggagaaaga aagttcaaag aaacggcaca
     7681 tccctctaca gaaggctccc ttgatatgta tcatattatg aaagcgttgg tgcaagaagg
     7741 gttcgacggt cctatacgcc ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc
     7801 tggatatggc ctgtatgatc gtgcaatggg actcacttac atgcaaggtc tatatgaggc
     7861 gataagtaag gaaaaaccac tgggagagca ttatgaataa aatcaagctc ttcatctttg
     7921 atatggatgg ccttttatta gaaactggac gtttagctta tcgtgcttat gtaaaatcag
     7981 cacaaaagta tgattatgaa atgcgtaagg aagtctatta tttactaact ggccaaacag
     8041 aaatggcgat aagagaacaa atgggcattt tgtacggaga agatgttcca tatatcaaat
     8101 ggcgagaagc aatcaatcaa tataaagaaa aaatcgtgaa agaagataag agagtatata
     8161 cgaaaaaagg agcagaagaa attttatctt ttgcaaaaga aagaggaatc catacaatcg
     8221 tggcttcgtc taatacacgg gtaaaagtag aaatgtattt aaaaatggaa aatctatatg
     8281 ccttgtttga tcaaatcatt tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga
     8341 tctttttgaa agcttgttca aaaatgaata ttcctccaag tgaagcactt gtatttgaag
     8401 actcgattgc agggatcgaa gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag
     8461 atcatttgat agggttgccc gatcataatg ggaagcataa gttgaaagta gatgtaagtt
     8521 tatatgaatc caataaagca gactatgaat tttatgacct gcatcaagca aaacgatttt
     8581 tagaaagtaa agggttgtat ctctaataaa gaggactatt ttctagtcct ctttatttat
     8641 tgtcctgcac gactgaggga acagatgaaa aagaagtaat tttttatttt gaagtatcta
     8701 ccttatttgt agtgaacaat ttagaacaaa cacaaaacgc atctactgtt atctaccagt
     8761 agatgcgttt aaagagaaga acgagaatcg tccatatgag ggtttccact ccttatgcag
     8821 atttttttaa ttcttgcact gcttgttcga tcgtaacacc tgtcgctaca tgattggtat
     8881 tttctttgtc aaaaacgaca aatagattca aatctgtatc caaggttact cgatattgta
     8941 tatctttgtt atagaatgtc atagccattc tcctttcttt aaatacttgc tgaaaggcaa
     9001 cgaacgcgtt gataacgaaa ttccatttat aagtatacca ccaattgaaa ggttttcata
     9061 gttaagaaat tttaaagtca aaagttgcag ataaaagcaa ataaacaaga aaacaaaaag
     9121 tgaatcaatc gcggttattc aactttttct tcgattcgaa gacaatagaa gacaatgaat
     9181 tttatatttc attgcattat ccttgtgtta ttagaaaaag catgatatat taa
//
LOCUS       DACDHJ010000093         9203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDHJ010000093 DACDHJ010000000
VERSION     DACDHJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.095"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..2172)
                     /locus_tag="IV223_001672"
     CDS             complement(79..2172)
                     /locus_tag="IV223_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8711963.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(2921..3544)
                     /locus_tag="IV223_001673"
     CDS             complement(2921..3544)
                     /locus_tag="IV223_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711964.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(3703..5100)
                     /locus_tag="IV223_001674"
     CDS             complement(3703..5100)
                     /locus_tag="IV223_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8711965.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(5132..6103)
                     /locus_tag="IV223_001675"
     CDS             complement(5132..6103)
                     /locus_tag="IV223_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8711966.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            6477..6902
                     /locus_tag="IV223_001676"
     CDS             6477..6902
                     /locus_tag="IV223_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711967.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(7390..8091)
                     /locus_tag="IV223_001677"
     CDS             complement(7390..8091)
                     /locus_tag="IV223_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8711968.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(8107..8547)
                     /locus_tag="IV223_001678"
     CDS             complement(8107..8547)
                     /locus_tag="IV223_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8711969.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(8547..9122)
                     /locus_tag="IV223_001679"
     CDS             complement(8547..9122)
                     /locus_tag="IV223_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8711970.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         2652 a         1891 c         1514 g         3146 t
ORIGIN      
        1 aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg tctaactact
       61 gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg ttcttgttac
      121 tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg tcgaatgttc
      181 gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt catatttttt
      241 cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt cctgattcag
      301 cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga tttcctcatc
      361 gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag cttttcctcc
      421 taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta cttgatcatg
      481 ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga ttcccaatag
      541 atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga ctgtcccttt
      601 agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt tgtcaaattc
      661 atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat ctgtattcga
      721 gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac cagatttttt
      781 caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc gaacataatt
      841 atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt catccgtata
      901 ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt gtagatgctc
      961 aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac ctaacgtttc
     1021 gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt ttttatatac
     1081 atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg tatagccttg
     1141 cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa gttctgtgaa
     1201 taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag catcacttgt
     1261 ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt gattctggaa
     1321 aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa tcaaaaactg
     1381 ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc caaatgtttc
     1441 gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa tcccattact
     1501 gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat aaatagtata
     1561 cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct gcagcaattg
     1621 tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg aaagacaggt
     1681 aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt tcttgacctt
     1741 caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga catcatgggg
     1801 cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa agcctaatac
     1861 agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca agtacaacaa
     1921 aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg gctttttaat
     1981 atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc acaagataaa
     2041 gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa tgatcgtctt
     2101 cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc acaatttttt
     2161 aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt gctcctgaaa
     2221 aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc taaagattat
     2281 tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact aatgaagaga
     2341 taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt ctcggtattc
     2401 gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct tgatgcagga
     2461 cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc ggccacaagt
     2521 cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg gctcagagtt
     2581 aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc tcggtattag
     2641 attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa gcttgcatct
     2701 gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa ttgctccttg
     2761 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
     2821 cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct
     2881 aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca taggattgat
     2941 attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca cttcccgtta
     3001 tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt ttttgagaag
     3061 agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa cgatcggcga
     3121 ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt ttcaaacggg
     3181 ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat
     3241 attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga ttttcttctt
     3301 ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt attttatact
     3361 ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta
     3421 cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg
     3481 ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc
     3541 tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa aaaatgaaat
     3601 agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc acaaaatggt
     3661 aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct caaatccgtt
     3721 tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt ttccatcttg
     3781 tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc acgaccagtt
     3841 atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa tggcttgatg
     3901 gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga tcatgccatc
     3961 ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa taaagcagtc
     4021 gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt gttcatagat
     4081 ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt cgaaataatc
     4141 ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta cccggcgtgg
     4201 ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg tcaaatcctc
     4261 tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt cacttggaaa
     4321 ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg ctatttgcgc
     4381 tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca gattcaagat
     4441 gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta acgctgaagc
     4501 caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt ctggataatg
     4561 ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag taaaccattt
     4621 ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat aggcttcaac
     4681 cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat cgaaatgata
     4741 cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac gatgatagaa
     4801 acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc tccattggat
     4861 agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct cttgatactt
     4921 cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt gcggttcttt
     4981 tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc ttccttctgt
     5041 ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat attttttcat
     5101 caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg atattttcca
     5161 tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac agctgttctg
     5221 ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga aggatactca
     5281 gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg attctgacac
     5341 cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg acaaaaatag
     5401 cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt ctagaatagt
     5461 ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct ttggctcctg
     5521 ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg atttttttat
     5581 gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca ttcactaata
     5641 ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga atactcattt
     5701 ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc agcccatcga
     5761 tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga tcggcaaacc
     5821 cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct gagacaactt
     5881 ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag cttcgattat
     5941 tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc gcttctgcta
     6001 tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa actgttccca
     6061 ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct cactcctgcc
     6121 agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc tttgcatagt
     6181 gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt cctctcacta
     6241 ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat ttttttaaaa
     6301 gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc tttttaaaat
     6361 tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt ccatccgttt
     6421 gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga agtattttga
     6481 agaaattaat cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt
     6541 tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg
     6601 aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat
     6661 ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac
     6721 aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatca
     6781 ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa acaacagtca
     6841 tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc tacagtctat
     6901 agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc
     6961 aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat
     7021 aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat atgcgacaac
     7081 tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt tcaaaattat
     7141 gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg atttttatga
     7201 acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac agtggatcat
     7261 ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa tataaaataa
     7321 ccaaacgata gaaaggctta ttctacatca cgtagtaatt tttctttccc gtcgtttggt
     7381 tttttatttt tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa aatattttcc
     7441 agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacggatca ccttttttgc
     7501 atcttctgga ttttctacaa aatcgaattt atccccatcc acttgtattt taggacatac
     7561 atcaaaatct tcataccatt gattatagcg ggtgttcaac gtttcataat aggtatataa
     7621 cgtctcatcg ctttctagct gttcatagtc acgcccacgt tttttgatac gttctagcat
     7681 cgtgtccaaa gaaactttga tatggaccat caaatcaggc cgtttttttg gagcaacatc
     7741 atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt cgatatccgt
     7801 tacccgacct aaatcagcgt ttagatggaa caacaggcta tcttcataga tggatcggtc
     7861 tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa
     7921 gaagatttgc aataaaaaag cgtactgctc aggatttgaa taaaagagcg gcaatacttc
     7981 gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa gcatatccgt
     8041 caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca tacaaccctt
     8101 cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc
     8161 acgtacagct tggtctgatt ttaaatacgt atggagactt tgtgtaatca tcaagttcgt
     8221 aggtaccgtc tcttcttgta caatgatcat cggttttttc aacatagttg ccacacctaa
     8281 ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag caacaacaaa
     8341 ttctgcattt gtcagttctt ccatatcttt ttcgtatact tcctgcgccc atccagcgct
     8401 gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg tgactgtttt
     8461 gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct ctgaaaaaaa
     8521 tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa ctgattttta
     8581 atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa gattacttga
     8641 tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa ttgttgcaag
     8701 ccgctgtaat aaccgccaat attcaatatt ccgattggct tgtcgggcgt cagaccgatt
     8761 gctatccaag aaagaagttg tgagaattcc tccaatgtac ctagacctcc agggaaaacg
     8821 aggacagcat ctcccttttc aaataataat acctttcttt catcgatcgt ttctgtagga
     8881 ataaatgtag taacatcttt tccaggcaat tcagtttcaa aaagcccatt cggatagata
     8941 cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat aatcccggag
     9001 tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc taacgtttgc
     9061 gcaataaatt tgtaactttc tttattacca aaacgggaac cgcaaaaaac agtaagtttc
     9121 ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt tttttaaaat
     9181 aaaagactag attttgtagt ttt
//
LOCUS       DACDHJ010000094         9157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDHJ010000094 DACDHJ010000000
VERSION     DACDHJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.096"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            16..597
                     /locus_tag="IV223_001680"
     CDS             16..597
                     /locus_tag="IV223_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711971.1"
                     /translation="MNEHFSVSFAENPSKYFRRLVFLLILSLLLTVAMFLLPEKIREE
                     QVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKTYQDMLDVFNDPELM
                     EELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRITAGKGGITDFDKEL
                     IPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1046..2095
                     /locus_tag="IV223_001681"
     CDS             1046..2095
                     /locus_tag="IV223_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8711972.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2092..2973
                     /locus_tag="IV223_001682"
     CDS             2092..2973
                     /locus_tag="IV223_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8711973.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2976..3746
                     /locus_tag="IV223_001683"
     CDS             2976..3746
                     /locus_tag="IV223_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8711974.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4025..4879
                     /gene="aroE"
                     /locus_tag="IV223_001684"
     CDS             4025..4879
                     /gene="aroE"
                     /locus_tag="IV223_001684"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8711975.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5057..6220
                     /locus_tag="IV223_001685"
     CDS             5057..6220
                     /locus_tag="IV223_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8711976.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6337..6747
                     /locus_tag="IV223_001686"
     CDS             6337..6747
                     /locus_tag="IV223_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8711977.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6921..9149
                     /gene="topB"
                     /locus_tag="IV223_001687"
     CDS             6921..9149
                     /gene="topB"
                     /locus_tag="IV223_001687"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8711978.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         3169 a         1573 c         1981 g         2434 t
ORIGIN      
        1 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
       61 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      121 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      181 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      241 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      301 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      361 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      421 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      481 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      541 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      601 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      661 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      721 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      781 tatttcagca gaaaacaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      841 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      901 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
      961 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1021 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1081 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1141 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1201 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1261 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1321 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1381 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1441 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1501 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1561 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1621 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1681 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1741 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1801 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1861 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1921 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     1981 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2041 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2101 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2161 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2221 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2281 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2341 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2401 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2461 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2521 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2581 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2641 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2701 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2761 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2821 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2881 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     2941 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3001 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3061 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3121 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3181 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3241 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3301 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3361 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3421 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3481 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3541 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3601 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3661 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3721 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3781 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3841 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3901 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     3961 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4021 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4081 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4141 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4201 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4261 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4321 tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4381 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4441 tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4501 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4561 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4621 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4681 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4741 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4801 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4861 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4921 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     4981 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5041 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5101 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5161 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5221 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5281 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5341 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5401 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5461 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5521 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5581 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5641 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5701 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5761 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5821 tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg ttcttttgca
     5881 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     5941 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6001 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6061 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6121 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6181 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6241 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6301 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6361 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6421 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6481 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6541 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6601 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6661 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6721 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6781 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6841 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6901 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     6961 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7021 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7081 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7141 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7201 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7261 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7321 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7381 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7441 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7501 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7561 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7621 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7681 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7741 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7801 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7861 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7921 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     7981 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8041 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8101 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8161 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8221 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8281 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8341 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8401 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8461 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8521 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8581 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8641 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8701 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8761 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8821 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8881 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     8941 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9001 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9061 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9121 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaac
//
LOCUS       DACDHJ010000095         8990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDHJ010000095 DACDHJ010000000
VERSION     DACDHJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.097"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            94..318
                     /gene="nrdH"
                     /locus_tag="IV223_001688"
     CDS             94..318
                     /gene="nrdH"
                     /locus_tag="IV223_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8711979.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            477..2636
                     /gene="nrdE"
                     /locus_tag="IV223_001689"
     CDS             477..2636
                     /gene="nrdE"
                     /locus_tag="IV223_001689"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8711980.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2686..3651
                     /gene="nrdF"
                     /locus_tag="IV223_001690"
     CDS             2686..3651
                     /gene="nrdF"
                     /locus_tag="IV223_001690"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8711981.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3740..4879)
                     /locus_tag="IV223_001691"
     CDS             complement(3740..4879)
                     /locus_tag="IV223_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8711982.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5188..5901)
                     /locus_tag="IV223_001692"
     CDS             complement(5188..5901)
                     /locus_tag="IV223_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8711983.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6155..6856)
                     /gene="nagB"
                     /locus_tag="IV223_001693"
     CDS             complement(6155..6856)
                     /gene="nagB"
                     /locus_tag="IV223_001693"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8711984.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7090..8622
                     /locus_tag="IV223_001694"
     CDS             7090..8622
                     /locus_tag="IV223_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8711985.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8813..>8990)
                     /locus_tag="IV223_001695"
     CDS             complement(8813..>8990)
                     /locus_tag="IV223_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8711986.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         2998 a         1748 c         1584 g         2660 t
ORIGIN      
        1 cacaatatgt agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata
       61 gataataaga atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat
      121 aattgtatgc aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa
      181 gaagtcaata tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa
      241 agtgttcctg tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg
      301 cgtaaacttg ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg
      361 cttgagacag caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca
      421 atgcaatgat ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga
      481 gtcttaaaaa tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag
      541 tcaatggtca aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa
      601 atgtcaaacc aaatacgatg acttttccat ctattatgga aaaaatcaat tacttgatcg
      661 atcaagaata tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc
      721 tatatcaatt tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat
      781 tttattctca atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag
      841 atcgagtagc cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc
      901 ttgcagacga aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg
      961 gacgtaaacg tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga
     1021 actctatcgg gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag
     1081 ggattacttt atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg
     1141 caagtggtgt cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat
     1201 taggtcagcg tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg
     1261 ctttcctttc agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc
     1321 ttggcgtgat cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt
     1381 tatttagtcc atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta
     1441 cagctgagta tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc
     1501 gtgatcttga aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca
     1561 atattgatac agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat
     1621 gttcagagat tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag
     1681 tattaggaac agatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa
     1741 gccctaactt tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg
     1801 catctgatat cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg
     1861 gattaggtgc catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag
     1921 aggattcatt agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg
     1981 aaagcaacaa tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt
     2041 atgctgacgg cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa
     2101 aagtcaaagc attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga
     2161 aacaagcagt aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg
     2221 gctcaatttc atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg
     2281 aagaacgcca agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg
     2341 agacgattcc gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt
     2401 atgcaacggc acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg
     2461 aaatcccaga aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga
     2521 atattttaag acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct
     2581 ttactgatga tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc
     2641 atagatagat tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat
     2701 tatgaagcaa taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag
     2761 ctgacagagc aattttggct agatacacgt atcccacttt caaatgacct agatgattgg
     2821 cgtacattgt ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg
     2881 ctagatactg tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc
     2941 catgaagaag ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat
     3001 tcttctattt ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca
     3061 aatacgaatc cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt
     3121 acaccgctag aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt
     3181 ttttatactc ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc
     3241 aaactaatca tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta
     3301 ggattcaacg agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt
     3361 ctttatgagc tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc
     3421 tggacagaag aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta
     3481 gggatggatc cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg
     3541 atttcaacag gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta
     3601 ggtcatgtag aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt
     3661 ttaattcaaa aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac
     3721 actgaactct tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact
     3781 tttccgaaac atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg
     3841 tcttaatcac tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc
     3901 ctgctccaat caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata
     3961 acaacggctt gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat
     4021 acaaagcttg aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa
     4081 aagggccgac atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat
     4141 aaggctgatc gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta
     4201 agatcgtact gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta
     4261 actctttacg aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat
     4321 ccttgaacat aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca
     4381 taaatgaaat gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat
     4441 tactaagaga taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt
     4501 cccgctgcgc caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct
     4561 gggtcaattg aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa
     4621 ctaaaatgat tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct
     4681 tttcgagaaa aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg
     4741 gtaaaaaaat tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa
     4801 taaagtacac gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt
     4861 gtttaaaacg tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt
     4921 taacaaagag ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat
     4981 attttaatga gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta
     5041 tactgcagtc cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc
     5101 cagccaaacg aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag
     5161 gacatctatt ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt
     5221 gatatttttg tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag
     5281 atcaatcgta tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt
     5341 cacatcttcg attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc
     5401 ttttctatgg ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt
     5461 cactactgtg agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc
     5521 catgccgatg gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc
     5581 cgtcaccgat tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac
     5641 cacttgagca atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc
     5701 taaagcatct cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc
     5761 tcccatacaa actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc
     5821 aaaaccgatt gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat
     5881 tgtcaatacc atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga
     5941 gcagacaaaa aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg
     6001 tcatagtcct ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca
     6061 aacggcaaaa tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc
     6121 tgatttcaca acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat
     6181 cgatgatcac aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta
     6241 caggtccttc aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga
     6301 cgatttcttt acttttcata attgaaccga ttcccattga aacagctttt gttggtacat
     6361 cttctgcttt atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta
     6421 ctgaagtcgt tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc
     6481 cgattcctaa gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc
     6541 atgctgcttc cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt
     6601 tatcaaataa ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc
     6661 caacgtactc atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca
     6721 tttctttata taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt
     6781 tgttcatacc ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta
     6841 cacgaatgat ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt
     6901 ctttttttct ttttacaata attagtatag accaaattgc atttttttca ttttcttgac
     6961 aataaatagg aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca
     7021 tgaaaaccaa gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa
     7081 ggagttatta tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc
     7141 ctctatgaag gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa
     7201 aacggagccg gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac
     7261 ggacagatcc attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc
     7321 gatgaaacat taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag
     7381 gaagaaaaaa ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg
     7441 gaaaaagctg ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc
     7501 ttagttgctg agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg
     7561 aaaaatctaa gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac
     7621 ccagatgttc tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg
     7681 ctgacgcagt ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa
     7741 tttcttgatc agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat
     7801 actggccatc taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc
     7861 caatatcatg ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa
     7921 gccggttccc gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg
     7981 ctgacgcctc cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc
     8041 acgacattag cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca
     8101 cccatcaatt tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt
     8161 ggaaaatcta ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc
     8221 cacttcccag ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca
     8281 ttagaaacac cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta
     8341 cgtcgccaac tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca
     8401 ttaagtggtg gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat
     8461 ttactgattc tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa
     8521 gacgggatac agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat
     8581 caagatcttg tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt
     8641 gaaaaaaagg agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc
     8701 caattagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat
     8761 ttggctattc ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt
     8821 tattttcgag atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt
     8881 gaaaaacaag cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc
     8941 ctagcaatga accagccgca cctccaataa agtacataaa cataaataca
//
LOCUS       DACDHJ010000096         8949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDHJ010000096 DACDHJ010000000
VERSION     DACDHJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.098"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..169)
                     /locus_tag="IV223_001696"
     CDS             complement(<1..169)
                     /locus_tag="IV223_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8711987.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQ"
     gene            354..1193
                     /locus_tag="IV223_001697"
     CDS             354..1193
                     /locus_tag="IV223_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8711988.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1389..2363
                     /gene="sgrA"
                     /locus_tag="IV223_001698"
     CDS             1389..2363
                     /gene="sgrA"
                     /locus_tag="IV223_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8711989.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            2661..3017
                     /locus_tag="IV223_001699"
     CDS             2661..3017
                     /locus_tag="IV223_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711990.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3020..4177
                     /locus_tag="IV223_001700"
     CDS             3020..4177
                     /locus_tag="IV223_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8711991.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(4203..4643)
                     /locus_tag="IV223_001701"
     CDS             complement(4203..4643)
                     /locus_tag="IV223_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8711992.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            4803..5309
                     /locus_tag="IV223_001702"
     CDS             4803..5309
                     /locus_tag="IV223_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8711993.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5477..7390
                     /locus_tag="IV223_001703"
     CDS             5477..7390
                     /locus_tag="IV223_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8711994.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            7395..8396
                     /locus_tag="IV223_001704"
     CDS             7395..8396
                     /locus_tag="IV223_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8711995.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(8598..8804)
                     /locus_tag="IV223_001705"
     CDS             complement(8598..8804)
                     /locus_tag="IV223_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8711996.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         3187 a         1335 c         1842 g         2585 t
ORIGIN      
        1 tttgattatt tgatttaact tttgggttat cataaaaatc ctgaactgta agtgttccac
       61 ctttaaacgt tgcaatcgtt tcattggact tgctacaacc tgagaatagt aaaattgcac
      121 atgctgaaag gcacataatt agtagttgtt ttttcaaaaa atttcccatc ctttccttat
      181 taaaaacagt ctaacagaag aattagggag acagtctttc attttcgtaa ggttgctgta
      241 gatattctta gttcgtaaaa atctattctc tctatgatgg agaacattta catagttttc
      301 acgagacaag aaagaaaagg gttgttaaac ttaatataaa ggagggatga aagatgatca
      361 ggattattcg gttgattgga tctgttttaa tagtcttagt tggttttgtt ttaggaattc
      421 ttgtcaataa tgcacttgcg gatatgttta ttggaaatag tgaatggatt ggagcaattg
      481 ccggtacggt cggacattta gctgtttttt tactagcttt atttcttgct agtaaggtcg
      541 aaggaaaaag aatagaagat tatggtgttt ctgttcgagg aaaagactgg gggtatttag
      601 cgggaggtct cgttgttgga attggagtgt ttctgcttat tactagtcca ttatatttga
      661 cgggggcgta tcggttagat tcaggaaacg caaacattgt gccgctgata acgagtttta
      721 ttttatttat tgcagtgggg gcatcagaag agcttttatt ccgtggcttt tttcagcatc
      781 agatgcttag atttggtccg ttaatagcaa tgattggatc tgctgcatta tttgctcttt
      841 tgcacggatt gaatcctaac atgacatttc tggcagtttt caatatcttt ttagcaggtt
      901 gctttttcag cgcacttatc tacagtacag gaagtttatt tacggctatt ggtgctcata
      961 ttacttggaa ttggacacaa ggagctattt tgggtatacc agtcagcgga acgcaagaac
     1021 aaggtttttt ctccactctg atccaatcgc aaaatacaat aattactggg ggaaactttg
     1081 gggctgaagc atctatatca actacattat tcttgttagt cttgattgtt ggtttgttac
     1141 tctatgcata taaaacaaag cggatgaaga aatacactgc tgtttatcaa taaaagatga
     1201 agaaagcaag gctgatatcg gtcttgcttt tttaagtctg tcattgatta aacgaaaaag
     1261 taaacaaatt caagcgaata cacgacatct tttgttagat tttcacaact gactgtcgtg
     1321 ttctcatcat tttcatttta tataattcct ttatcaaata gaaggtaatt atatggagag
     1381 tgatagaaat gaagaaaaca gcgaccgtat tatttgttat tcttgggctg agtataattg
     1441 cagcaatgat tggaatcagt acttctgcaa atgaacgggc aaatgagaat ctttcattta
     1501 ctgtgaaaac tgatcggatt gtttatgaca tgacacaaca agtcattact attccagtca
     1561 aaccaaacaa aagcgtgaat gctagtgatg tacatgctgt tctaacatat ggttgggatg
     1621 gtaatggaag ttcagaaaaa gttattgggg aagtttattt aaaagacgta caatggaccg
     1681 ctggtattga gtatacaata atgatttctg cagaactatc gattgatgag atcaagagta
     1741 aagataaagt tgatttgatt gttttttatg atggacagat gacgatcacg gagaatttga
     1801 aaccaagcag ttggacagtt gttggaccgc caagcagtac ggatagcaca agtagtgaaa
     1861 gcagtacaga aaacacgagc ggtgaaagca gtacagagag tacgagcagt gaaagtagca
     1921 cagaaaacac gagcagcgaa agcagtacgg agagtacgag tagtgaaagt agcacaggaa
     1981 gcacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcatga
     2041 gtagtgagag tagcacagga agcacgagca gcgaaagcag cacaggaaaa gtaaataatg
     2101 aacttgattt ggtacctatc ataccaacta aggaacaaaa gagaaatgca gggagcaacc
     2161 taaaaagtgt gctaaaacag cctgattttg tttctcgtgg aattgatcat caagaaaaag
     2221 cgaaagaaac agataataga aaaagcgatt taccagaaac tggtagtaga accttgaaca
     2281 agctaattac atggatgggt gtgttactca ttttgattgt aggagcaagc tattttcgtt
     2341 ctctcttcca tcgagtgaaa taaagaataa aagcaacaga tctaaaattg aataaaacga
     2401 tagatttttg agtgaatatt tcattcgaga gtgctgtatt gcctggcgaa gttgtagctt
     2461 tgccagatct atttttgttt actaacatat ttgagaaaca ggaaataaca ggaacacaaa
     2521 tggaaaataa gagcaagaat aattgacaat gcacactgcg catggtagta tctctgaaaa
     2581 atcagtaacg aatctatttg tacgttattc atgtagctgt cggaataagt ctaaataata
     2641 gataaaaagg agtatgtact ttgtttgaag aatttattga tatcaatgaa aggcaagtat
     2701 atcaattttt gaactattgt tatgaaagag acgagaaatt atatgtggtg aaggatatcg
     2761 ctttagatct aaactacacc ctggctaaga tgaattcggt gattcaacaa gcagaatcgt
     2821 tttgcgaaag gtatccagaa tataaattat cttttctttc agaaaacaag atgataaaag
     2881 tcgagttttc ttctcagttt ttactgagta aagtttattc tatactttta gagggaacga
     2941 ttggttatat tttactggat tcactttaca aaggtactta tcaatcctta gaaaatctta
     3001 gtcaaaaaat tatataagtt tgcgtacgat tcaaagaaaa ttaagagaac ttaatactat
     3061 tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt aatccgatag aaggagaaga
     3121 ataccgtatc cgatactttt ttcacttgat gtattggcag tcattcgacg aaaatcattc
     3181 tgatatatta tcaagtataa aaaataaacg tcaactatta gaaaaatttg cgaaacatgc
     3241 gccatatacg cgtagtatag atagaaagaa atggatcagc ctactaagaa tttcgttgca
     3301 acgaatgaaa caaggccact cgattacgta tgtcccagaa gagatgaaca aatttgtgca
     3361 tccattgata acaaaagaag agtttacgca ttacatacta gagccttttt ttgaagcaaa
     3421 cttctggtcg atgaatcaca tcactccaac agagcaagct tacctttatt ttatgtttgg
     3481 tgtaatgaac acctatttgg aagaagattt gttggatact ccagataaag tacaagaaag
     3541 ttggggctgg attgaatcgt ctattgtaga aaattttgaa aaaaacttca agatttgttt
     3601 tacaacttca gaaaaaaatt atctatatgt caatttggca atgatccatt tgtattcaag
     3661 ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa tcagcagaag acagagattt
     3721 tcagcatata tttccagtga tgtatccaat catgcaacag ttttttagtg gggtagctcg
     3781 cagaattcca gagcttaata cttattatga aaagaacaga cggttgattt ttcaatactg
     3841 tatgttagtc agggatattt ttataaaaca cgaacaacct gtcatattct atattcaatc
     3901 aaagtatggt aaaacacagg aattatgggc aaagaaaagg atattatcta tgactgaacg
     3961 cccaataaaa tatagtgcat ccgctgatca gttacctgat attgtcatta gcgattatcc
     4021 tatcgacaaa aaggcatatg atcaaacgaa tacacagata ttttactgga atggacaacc
     4081 tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata aaaatcagga atgagaaaaa
     4141 tagatccata ttggaaaaat tggatttcgt taattaagaa aagcagattg tttatgccaa
     4201 aattaagaca ttgcaaaaag attattttct ttaaaatcga caagatgacc agaaagcaag
     4261 agttcgttga gctcttgctt tcttttagtt ttagaaagga acatcctgat tttatcttcg
     4321 ctaaatccag tcaaaagttt tgcagattgg tcgtagataa tcggtttttt caataaagaa
     4381 ggggatgtac gaatctcaat atatagttca ctcaaactga gttcatctat tctgtgaaca
     4441 agatttttat aactaataga atgagtagag agcatttcgg tcacgccgtt gtttgtcaat
     4501 tttagtaagt ggataaattc atttttggtt agtggttgac gaattaagtt ctgttcgata
     4561 aacggtatat catggacgat aagccaacgt tttgcgcgtc ttgaagacga gcagccagaa
     4621 cctgtataca atttaatcat catacattcc tccaatagct aagtatttat gaaataataa
     4681 catgaagaaa tagtcgaaaa gtgccattta gaggtgtaaa ataagaaaat gttgtcacgt
     4741 ataatggaac tagctagata agaaatacta ttaaatcaag aaaaaagaaa gaaggcacaa
     4801 gtatgtcaaa cacagccgac gcaatagtag tgatcgattt acaaaaaggg gtatgttttg
     4861 attcttcgcc gatacacgaa ttagaaacag tgacagcatt agtgaataaa agaatcaaaa
     4921 catacgaaac gattgggaaa ccagttattt tcgttcaaca cgaagatgct caattagtgc
     4981 cacaaacaaa accatgggag ctattgtcgg aattatctat tcctgagcac agttattttg
     5041 tgagaaaaac ccatgcgaat tcattttata agactaattt gagtgtttta ttaactgaat
     5101 tggactgtca ttcgatcgaa ttatgtgggg cacaagcaga gtattgtata gatacgactg
     5161 tgaagtttgc tcatggatta ggataccagc ttatgatgtg tagaggagca acgacaactt
     5221 atgataatcc ttttatgaaa gcaaaggaaa caatcgcttt ttacgagaaa atttgggata
     5281 aacgatttct gacatttttg gagcaataag ttgtttcttc gtttaaaaaa agcagaatgg
     5341 atactggttt atccattctg ctttattacg taaaatgaga aaataatcct gttctatttt
     5401 tatgggataa accatttatt ttttgtataa aaaatgtttg cgtttacata taattctcct
     5461 agaaacataa aaaggagtga atagcttgag aacaaaacaa gtattaaagg gagcgttggt
     5521 catcttattt gctgtaagta caagcgtccc gcaatggaca gtatatggag agagcatttc
     5581 ttccgaatcg gaatctcggg aggtagaaac cacagaaact actgatgctg gaaatgaaac
     5641 gaaatcgagt acttcacaaa cagaaacaaa tactgaaaca acggttgaga catctgttac
     5701 tagcacagat caagaaacga ccgctaccac agataaccaa caaacagaag aagcaacagc
     5761 tacaaaaatg tcaaacgacg aatcctcttc tgctaaagta agtgacaaag agaatgggac
     5821 agaaaatgat gatatcggta aaggtgaaaa tttcaatggt aaagaatatt tgaattactt
     5881 cggtaaacag atgacagaaa tacttaatgg accaacagaa gacatcaagt caaaattaat
     5941 agagttatta gtcactgagc ttgctgttaa tgattatggt gtagataaag agattatcaa
     6001 acatctgatt gctacttatc tattgtacga tatcgctttt gcaaaaaaca gtgtgttgat
     6061 gtcgatgctt ggaggtacag atgatgggac aacgcctaca gaaaacacag gcttctatcc
     6121 aaatacaaaa caacttcatc aaactgtact aaacaaacaa gatggcaaaa tgatggatgt
     6181 atatgcatat tatgtcgatc aaggttcaga taaaacagtc atgatccatg gcggatttcg
     6241 agggaattgg aataacggga tcgttacaga agaatacaat gatttttata aggccgggta
     6301 taatttatta tttgttgatt ccagagcgac tggtaatagc ggaggcgatt atgtgaccta
     6361 tggacaatat gagtcagatg acgtccttta ctggataaat caagaagttc gagagcggcc
     6421 atctcaaaag attttacttt atggtggttc gatgggagca gctacgatga tgtctgtatt
     6481 agcgaaagat attcctgtca atgtcaaagg aattattgaa aattgcggat ttgcatcaat
     6541 tgatgaacaa ctacgtttta cgtacagcca gacagtagcc cccgcactac cggatgccat
     6601 aaaaaatcag ttagacatca ttggggatca agagcatgaa gatttattta tgggcctttt
     6661 gaaacaatac tattttgatc aggagatgca tttggataca aaagctgctc tacctacgat
     6721 tggaatgtca gattcattgc ctaaattgat tatccatgga acagcagatg atgtagtacc
     6781 tgtgtctaac gcgcaaaaat tatatgagtt atcaggaggg tataaagatc ttttattagt
     6841 agaaggagca gggcatggaa aggcacagga agttgaccat gctgcctaca cgaaacatgt
     6901 aacggacttt ttgaaagtag tgttccatga tcagataaac gtaaagtatg tggatgaaaa
     6961 caataaaagt ctactcaaag accaggacga aatccagctt tacggagctt atggagaaaa
     7021 ttatatgaca gagcaaaaga cattcgaagg atacgaatta gcaaatgttg aaggaccgac
     7081 tgaagggatt tttaatgaga cgataccaac tattattttc tattacaaaa aaatcccagt
     7141 agctccacca aaaaagcaag atccgacaga gaatgcagac aataagggaa agaatgaacc
     7201 tcgagaggat caatcaagtg atgatacgca accgattcag atgacagtga aagtacaaaa
     7261 aaagacagat gcaaaaaaaa gaatgttgcc gaaaactgga gaaaagaaac attcgcttct
     7321 catttattca ggtggatttt tatctgttag cacgattatc acatggattt ggcgaagaaa
     7381 gagagggtaa atgaatgaaa tggttcaagc gaatagtgat tgcagtacca gttatcatcg
     7441 tagttatttt agtcactgta tttttgatta atcaaatcac accaaagccg attagcttgc
     7501 ttgttagaaa acaatttgac acaagtgaaa aagaacaagt atatgcccga ccagatgatt
     7561 tccagcaaaa aatcgaacaa atcgatataa aaaaagaaat atcctatcct tcaaagtaca
     7621 aaaataataa tatggatatc tacacaccga aagtggaaaa gaaaaaatta ccgattcttt
     7681 tctggatgca tggaggcgca tatgtaggag gggataaaaa tgattgtcgt gattatttgg
     7741 aatatctatg ttcagacacg cagcaaatca tcgtaaatat agattatgaa cgatcaccgg
     7801 aagcaaaaca tcctacacca gttatccaat tgaacgaagc cattcaagca gctaaaaaag
     7861 agataaaaga tcaagcagat tggtcgaata tttcgattgg tggaaattcg gcaggtgcac
     7921 aaattagtgg agaatatttg ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc
     7981 ttgatccgac tttggagaac aagcagatta cgaagtttat ttctttgtct ggtctgttgg
     8041 atccttctgc acttactcaa gtaagtgata aaatctctag ctttctttat gaaaaatgtg
     8101 gatgggctta ctttgataat aaggactttg aacaatcaga gaaggtcaaa agtttagcct
     8161 tggtcagaca tgcggatcgt tggacgcaaa acacattctt aacggatgga aatacaaata
     8221 cgtttacgaa acaaatggaa gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca
     8281 caaaagtaga ttatgcggag aaaaatgctg ttttgaacca tgaatatcag tttgatttca
     8341 gcaataaaca agcgcaagaa acatatcaaa agttagtaat gtttttcaag gagtaaagtc
     8401 ttatgatgca aaagaggttg taatctgtca cttagctcca agtcacaagg agcaatttca
     8461 ataaacaagc ttttcatggt ttatgaatat tgtaacttgt gactgaggag ttagagatgg
     8521 caaggggcac cttttattcg aaatttggga atatcacacg attattgtca cgatctcttt
     8581 tacgtaaatt ttataagtca gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg
     8641 aagcctgcgg aggcaagaag ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc
     8701 actaatattc cgttgttatt gatataaggc tgggaacaaa gatgaatagc aaataaaatc
     8761 aatgcactag cgaaaaaaag caaagaaata tggatagctt tcataagcag cacctccttt
     8821 tttataattg tatgataaat agattaagaa tctataaatg tgacaagttt gttaggatgt
     8881 gaataaaatg atcatctagc gaacttgaaa caaagatggc taatgataag caattaatga
     8941 ttcagcaat
//
LOCUS       DACDHJ010000097         8783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDHJ010000097 DACDHJ010000000
VERSION     DACDHJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.099"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IV223_001706"
     tRNA            2..74
                     /locus_tag="IV223_001706"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IV223_001707"
     tRNA            78..150
                     /locus_tag="IV223_001707"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IV223_001708"
     tRNA            155..225
                     /locus_tag="IV223_001708"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IV223_001709"
     tRNA            238..311
                     /locus_tag="IV223_001709"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IV223_001710"
     tRNA            323..394
                     /locus_tag="IV223_001710"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IV223_001711"
     CDS             660..1853
                     /locus_tag="IV223_001711"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8711997.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IV223_001712"
     CDS             1983..3455
                     /locus_tag="IV223_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8711998.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IV223_001713"
     CDS             3538..4326
                     /locus_tag="IV223_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8711999.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IV223_001714"
     CDS             complement(4393..5061)
                     /locus_tag="IV223_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8712000.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IV223_001715"
     CDS             5176..6888
                     /locus_tag="IV223_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712001.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IV223_001716"
     CDS             6891..8663
                     /locus_tag="IV223_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712002.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2636 a         1493 c         1952 g         2702 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg att
//
LOCUS       DACDHJ010000098         8744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDHJ010000098 DACDHJ010000000
VERSION     DACDHJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.100"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(240..2621)
                     /locus_tag="IV223_001717"
     CDS             complement(240..2621)
                     /locus_tag="IV223_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8712003.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKLAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2611..3258)
                     /gene="recU"
                     /locus_tag="IV223_001718"
     CDS             complement(2611..3258)
                     /gene="recU"
                     /locus_tag="IV223_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8712004.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3322..3861
                     /locus_tag="IV223_001719"
     CDS             3322..3861
                     /locus_tag="IV223_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8712005.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3951..4370
                     /gene="gpsB"
                     /locus_tag="IV223_001720"
     CDS             3951..4370
                     /gene="gpsB"
                     /locus_tag="IV223_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8712006.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4392..4759
                     /gene="rnpB"
                     /locus_tag="IV223_001721"
     ncRNA           4392..4759
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV223_001721"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4968..6134
                     /locus_tag="IV223_001722"
     CDS             4968..6134
                     /locus_tag="IV223_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8712007.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6180..7676
                     /locus_tag="IV223_001723"
     CDS             6180..7676
                     /locus_tag="IV223_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8712008.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7757..8440
                     /locus_tag="IV223_001724"
     CDS             7757..8440
                     /locus_tag="IV223_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8712009.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2756 a         1582 c         1853 g         2553 t
ORIGIN      
        1 aaagactagt gtgtatttag ccgatttaat tataaacaag taaatggata aagtatatgt
       61 aatgataaaa gaaaaatgga ttttctaatt tttaagctaa atgttagatc acacatgtat
      121 atgctggata gtcttataaa aaatcatgta atacagcttg gtacatgatt tttatttgta
      181 gctgtttttt acatagacac aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac
      241 tatctgtttc cagaagctga ggtgctgctg gatggtgtat ggtcatttgc agcatccgct
      301 ccgctagttg ctggctcact ggaagaagca ggaggagcag atgaagacgg tgtcgagctt
      361 tcttcggatg aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc
      421 gtgctttctt ttgaggactc agcagttgat tcgctttgac tagtagagct agagctttga
      481 gtcgttgtct ctgttgttga gcttccagga gtctggacat agctggaaga tggaatagtt
      541 gttgatggtg taattgcatt cgatctagct gtatattgat ctttgtagta cagttcaccg
      601 cccacacgaa ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca
      661 tactgcataa gctcgcgata aacatcagag gctacgtgtg acgattctga agtgacaggt
      721 gtcatttttt tattgtaacc tgtccacacg gaaatcgaat aatttggcgt atagcccgca
      781 aataggatat ctggatacac cccagaagaa ataccaagtt ttgcatactc gtcatctgtg
      841 taattggatg ttccggtctt accggcttga tataacccag cgatttgcgc attcgtacca
      901 gttccttcag tgatcgtatc ttttaaaatg tctgtgatca tataagctgt ttctggagac
      961 atggctgttt ttccatctgg ctcataagtt tcttctgttc cgtcttggaa gacgatttta
     1021 ttaacatatt gaggtttgta ataagtccct ccattggcga aagccgcata ggctgctgcc
     1081 aattttaagg aagaagctcc gtattttgtc ccatcttgtt cttccgtatt actagagatc
     1141 gcattcgatt gatggatcgt actgtattcg atcccaagat tttttaagaa agaagccact
     1201 ttatctgaac cgacttcatt gaataatttg actgctggaa cattacggga taagtaaagt
     1261 gcttggcgta aagtaatggt gcccatatat tgattatccc agtttcctac aggagtcgaa
     1321 gtcccttcat agttatacgg tgcgtcagta atagtttttc cagtagaata tttcaaatat
     1381 tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta
     1441 ttcactgcta agttgttgcc taaagttacg tcttctgcga tgtgacgtcc gccaatctgg
     1501 gcttttactt tcccggtatt ggtgtcgata agggtggagg caacttgcat ctcgtcgtct
     1561 gggtaagaga catactgatc cgtgttgacg atatcatata gtttcttttg ggcgtccaga
     1621 tccaaattcg tataaatctc taaaccgtct gtgtagacat tcttatctgt tttttcttgt
     1681 acttcattga tgacttcttt gacataatta tcgacgatct tagtattgtc atcggattga
     1741 gttagttctt gcagaccatc cgttaccggt acattaacag cttgatcata ttctgtttga
     1801 gagatttttt cgttttgaag catcgtatac aaaacagtat ctcgtctttt cttcgcctga
     1861 tctggataaa cataaggatc ataggcggaa ggggcttgag gcatcccagc aagcaatgct
     1921 gtttgcggta aagaaagttc tgataatttc ttaccaaagt acatctcaga agctgtctcc
     1981 atcccgtaca agccgtttga catatacact ttatttacgt agtaggtcaa gatctcttgt
     2041 ttcgattttt tttgttccaa tcttacagcc atccacgctt cttgggcctt gcgtttcaga
     2101 gtctgatcct cggcactggt ggagaagaag gaaagtttga tcaattgctg tgtcaaagta
     2161 cttccgcctt gcagtccgcc agaggtaaag ttagagaggg cagaaccgat gatccgaata
     2221 gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat cgcatcttct
     2281 aatgttttcg gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg
     2341 aatagttcgc catcttgtgt atatagtttg gaagatacgg tagcgtctaa ttttttatcc
     2401 gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg
     2461 atacagaaaa atgagagtat tcctaaaagg atcttgataa ataatccttt tttatgtgtt
     2521 ccagaggact ttccagaacc tttgccagaa ttcttcttca cactcttttt aggagtaggc
     2581 tgtttctgtc gtctagagct tctagtttgt tcatttgcca ttcttgcttt ctccttcaat
     2641 agataatgtt gataaataga agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc
     2701 agttttgatc tctattccta gttcttcgat caaagataaa ggtagtgatt tgcgtccttt
     2761 ttccttttgg gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa aacagcgctt
     2821 caaagacgaa aaccataaca agacaaagca gaccccattt tgttcgaggc atgctttcat
     2881 atgttcgatt tgatgggcat ggaagttttt aaagggaaaa gatgttttgt tcttggtttc
     2941 ttttgcttca aagtccaagt aaaagcctcg atagacgcca ttatagtctg tagttgaggc
     3001 ttgggtaaag taggcttcct tgatcacagc agcacttctc ttaggatagt ctactttcac
     3061 gatttggata ggggtaggct ttttatggat aaccgccaac ccgcgggaaa gataatattt
     3121 attactttca ttgattgctt cttcaaagcg catcccccga ttgccgaatt cagtgatctg
     3181 atttcttttt tcttgaattt tttcgacggg agaggcattc ttataataag gttggccatt
     3241 tggatagcgc aataccatct ttaatcacac tcctaggcca cattatagca aaaaagcgga
     3301 tagaaagaaa aggaatctga gatggataag tcaaaagtaa tctatgtgtc tgggtatcga
     3361 agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt
     3421 ttaaaaaaag aactccatca gttagcagaa gaagggctgg aatgggtact tgtcagtggg
     3481 aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga agaatatccg
     3541 gaactatctc ttggtatttt gtatcctttt tctgattttg gtaataactg gaatgaaggg
     3601 aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc tgttagccat
     3661 caaccctacc aatcacctag tcagctaaag aatcacactc gttttatact tgagcataca
     3721 gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa aaacgaagta tttttttgaa
     3781 gatgcacaga attaccaagc agatcatccc tacgaaattc ggctgatcag tatggacgat
     3841 cttcaaaatt ttagtgaata atcgatattg tgtttgattt atcaactttt ttcctataca
     3901 atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat atggcgaatt
     3961 tggtttatag tcctaaagac attttacaac aagaattcaa aacaaaaatg cgcggctatg
     4021 atcccgttga agttgatgaa ttcttagaca atatcatcaa agattatgaa acttacagca
     4081 aagaactcct tgctttacaa gaagaaaacg atcgattgag tgcaaaagta gctcaattat
     4141 ctaaaactca aggagcagct caaacacgcg tgcaacaaac agaggcacca aagagcgcag
     4201 cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta
     4261 aaaagcttga tcaacaagca agtgcagtca aaccagcaca acctaatcca aataactaca
     4321 caaatgcaga cacaagctta gatgataatg aaaaaacacg tcaattttaa tttttgatta
     4381 atgaatttgc ggttttcggg taatcgcggt ctgacttgat caggctgagg aaagtccatg
     4441 ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag ctaaggtact
     4501 ctttggagta acggcaggaa aacaagctaa ggcttcggct atgcttaagt atccttgaaa
     4561 gtgccacagt gacgaagcgg ctggggaaac ccatccggtg gaacgcggta aacccctcga
     4621 gcgagcaacc caaacaatgg taggggcgct ttttgtctgg aagaagaaca ggacaaaaag
     4681 gcagtataac tgcagataga tggttaccgc tttgtttttt ctccctggaa aagcaaagta
     4741 cagaacatgg cttatagaaa accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg
     4801 cgttataaga aacaagaaaa acatttgaaa gctatttttc aatacttcag atgaatcgtt
     4861 cttattacaa aacaaacttt tgtatcaccg gttattcgga atttgagtaa aagtgtgggg
     4921 taggactgat gtcccacctc ctttttttat aaataaggag attgtgtatg gaaaaaaaca
     4981 ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga agcactggtt ggtaaagaat
     5041 taagagatct tggaatcgaa tgccaagttg agaacggacg tgcacgtttt gaaggaacaa
     5101 tggaaacgat cgctacagct aatttatggt tacgtacagc agatcgagta aaaatcgtgg
     5161 ttggggaatt cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa gcattgcctt
     5221 gggaagattt tcttccacta gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga
     5281 aactatatag cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc
     5341 gtacctatta tcaccgtcca gcttctgtac cactgacgga aactggcgca cattttcagt
     5401 tggaagtagc tttgctgaaa gaccatgtga tggtaactct ggatacgaca ggaccaagtt
     5461 tattcaaacg tggttatcgc ttagaaaaag gtggagcacc attgaaagaa aacatggcag
     5521 cagcacttat tatgttgaca aattggcgaa aagaccgtcc attttatgat cctgtatgtg
     5581 gttcaggtac gctttgtatt gaagcagcct taataggcca taatatcgca ccaggattca
     5641 atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg gaaaatgttc
     5701 gatcagcagc cgaagaaaaa gcagattatg atattgaatt agatattact ggttcagata
     5761 tcaatggacg gatgattgaa attgccaaag ccaatgcaga agaaatcgga ttaggctctt
     5821 cgattacctt taagcaacaa gcagtcaaag acttcaaaac ggataaagaa tacggggtca
     5881 tcgtggctaa tccgccttac ggagaacgtc taggagaaga agaaactgtt cggaaattat
     5941 ataaagaaat gggcgatgta ttccgtccat taaaaacatg gagtaaatat atcttgacta
     6001 gcgacttagc gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga aaattgtaca
     6061 atggtgcatt gcgtacggat ctattccagt attggggaac tcgtccgcct agaaaaaaag
     6121 agaaagacga ataatcttac taattgattc attcaattgg atgtttggag gaatagggaa
     6181 tgaaagaaaa agagttttta caagaagtaa aagaaatcaa tcttctacaa caagctttag
     6241 gaatcctcga ttgggataca caaacaggta tgcctgaaaa agctagtgca taccgaagtg
     6301 aagtagacag ctatctttac agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag
     6361 aagccattcg ctatttttct gaacatccgg aagaattgtc ggaagttgga actgccgttt
     6421 ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt accggaagat cttatgagaa
     6481 aatatacagc tgccgtttct tcagcacacg gacaatggca aaaagctaga gaaacacaac
     6541 aattttctga ttttcaacac gcattgaatc aaaatattga attaacaaaa cagttgatac
     6601 cttactggag aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa tacgaacccg
     6661 ggatgactgt cgaaatcctt gataaggtat ttgatcagct taaagaagga attctttcga
     6721 tccgccgtac gctccaagaa aaagggactg aacctgaaac agacttttta aatcggagaa
     6781 tgacgaaaaa acaacagaag agatttgtag tgaaagtaat cgaacaactt ggttatgatt
     6841 ttagtcgtgg tcgtttagat gacactgtcc acccatttat gactggaatt aatccgaatg
     6901 atgcacgaat cacgacacga tggaacgaaa cagatttcaa aatggctgtt tttggagtga
     6961 tccacgaggc tggtcatggt atgtatgaac aagacatcga tgataaatat gcgtatacac
     7021 cggtttatga aggtacatca atgggtatcc atgaatcaca atcgttgttt aacgagatca
     7081 ttattggaag tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag
     7141 aaggaacatt cgatgatatt ccttttgaca aattttatcg ttcattgaac catacaaaat
     7201 caagtttggt tcgtatcgag gcagacagtt tgacttaccc tctccatatc atcattcgtt
     7261 atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta cctgaaatat
     7321 ggaatcaaaa atatgaagag tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc
     7381 tacaagacgt tcattggtca ggtgcaagct ttggttattt tccctcttat gcattagggt
     7441 acatgtatgc ggcacaatta cttcattctt tgcagcaaga aatagatgtg gaaaatgttc
     7501 tagcgtcaga cgattattct ccaatcaaaa actggatgtc agagcatatc catcaatatg
     7561 gtgcatctag aaaaccaaat caattaatca tggatgcaac acatgaaccg cttaatccgc
     7621 aatacctgat cgactatatg aaagacctgt attttaatgt atatcaggta aaataaaata
     7681 caatttacaa agatagctga aattcatcta atctatagat gttttcagct atttacttaa
     7741 gaaagaagtg gaacgaatgg ataaattaca agtgatcgct gattatagta tggaccaatt
     7801 agcgcatgat caaacaggac atgggagcga tcataccaaa agagttgtca agttagctga
     7861 aaaaattttg gagacggaac cgcaggcaga ccgctttgtg acgcttgcag cagcttacct
     7921 acatgatacg attgatgata aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg
     7981 tgtattcctt cgctctttgc caatagtaga ggaacagatc tcgatgattt tttccattat
     8041 cgaaaatatg tccttttcta aaaatctctc tgaagcggcc gaactatctc tggaaggtaa
     8101 gatcgttcaa gacgctgatc gtatcgaagc attaggagca atcggcattt tgcgtactgc
     8161 ttatttcggc ggtggacatg ggcacccgat ttttgatcct gaactgtatc cgcaaacatt
     8221 caaggacaaa gaaaattacc gaaaaggcac gacagtgatc aatcattttt atgaaaaact
     8281 gttcttgctg tctgataaaa tgaatactga ttacggatat gaagaagcca aacgaagaga
     8341 aaccttcatg cgtgaattct tagaagaatt ttttctggaa tggtctattg acgatgaaaa
     8401 ttttactcca gacaattgga tcaaaaataa caaaaaatga tggaggttgt gacagaagtg
     8461 gtcagctcca agatatttcg gatcattttc cgtaaaagtt acttctggaa cactgtttac
     8521 caagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc aaaaaatagc
     8581 ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat tggcaggaga acaccgttta
     8641 ttcggaagaa taggactgtg acaagacttt tgtcacagtc cttttttcga attcaacaag
     8701 taaaactttt aagaaatatt cagagagtaa aattaaaaat tgct
//
LOCUS       DACDHJ010000099         8603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDHJ010000099 DACDHJ010000000
VERSION     DACDHJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.101"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IV223_001725"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IV223_001725"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8712010.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IV223_001726"
     CDS             complement(1098..2201)
                     /locus_tag="IV223_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8712011.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IV223_001727"
     CDS             complement(2291..3226)
                     /locus_tag="IV223_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8712012.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IV223_001728"
     CDS             3528..5462
                     /locus_tag="IV223_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8712013.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IV223_001729"
     CDS             5919..6335
                     /locus_tag="IV223_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8712014.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IV223_001730"
     CDS             6349..6915
                     /locus_tag="IV223_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8712015.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IV223_001731"
     CDS             6938..7924
                     /locus_tag="IV223_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8712016.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="IV223_001732"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="IV223_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8712017.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1657 c         1752 g         2667 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DACDHJ010000100         8600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDHJ010000100 DACDHJ010000000
VERSION     DACDHJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.102"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(5..409)
                     /locus_tag="IV223_001733"
     CDS             complement(5..409)
                     /locus_tag="IV223_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712018.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(402..803)
                     /locus_tag="IV223_001734"
     CDS             complement(402..803)
                     /locus_tag="IV223_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712019.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(793..1146)
                     /locus_tag="IV223_001735"
     CDS             complement(793..1146)
                     /locus_tag="IV223_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8712020.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1136..1447)
                     /locus_tag="IV223_001736"
     CDS             complement(1136..1447)
                     /locus_tag="IV223_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712021.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1444..2319)
                     /locus_tag="IV223_001737"
     CDS             complement(1444..2319)
                     /locus_tag="IV223_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712022.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(2329..3489)
                     /locus_tag="IV223_001738"
     CDS             complement(2329..3489)
                     /locus_tag="IV223_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8712023.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3489..4175)
                     /locus_tag="IV223_001739"
     CDS             complement(3489..4175)
                     /locus_tag="IV223_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8712024.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4138..5316)
                     /locus_tag="IV223_001740"
     CDS             complement(4138..5316)
                     /locus_tag="IV223_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8712025.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5336..7030)
                     /locus_tag="IV223_001741"
     CDS             complement(5336..7030)
                     /locus_tag="IV223_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8712026.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(7008..7322)
                     /locus_tag="IV223_001742"
     CDS             complement(7008..7322)
                     /locus_tag="IV223_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8712027.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7425..7706)
                     /locus_tag="IV223_001743"
     CDS             complement(7425..7706)
                     /locus_tag="IV223_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712028.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(7711..8055)
                     /locus_tag="IV223_001744"
     CDS             complement(7711..8055)
                     /locus_tag="IV223_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8712029.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(8081..8248)
                     /locus_tag="IV223_001745"
     CDS             complement(8081..8248)
                     /locus_tag="IV223_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8712030.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
BASE COUNT         2476 a         1800 c         1247 g         3077 t
ORIGIN      
        1 cctcctattt atttaattta tcgaattgac gaatcagaag ttctgtaatt ggatcaagtg
       61 caagacctaa tcctcttctc ataaattcgt ccggctgatt tcttttagaa gtacctatcc
      121 ccaaatcagg atattttaaa tactcaaatt ttcttgtagg tctaatgatg aaacccaaat
      181 taatgtattg agtcttaagt ggacgactat tttttgcgtg ttggtgccct cttcttaaat
      241 ctgcttcaga aacaggaatt ttttctgtaa tcctatccac tgcaatagcc gaaccttttg
      301 atttcaatgc ttcgttaatc agtcgttcgc tctcgcttga atagcgttcc atccgcacaa
      361 gaagttcatc atgtccattt atttttagct cccaactatt tttagccatg acaatcactc
      421 ttcaataatc gtctaaacgt aaataccaat tgatcgatat agcgatcttg gttctctagt
      481 tttaaatgat tgggatccat tctctgaaaa cgaatcgaac gattttgaat caatgaaata
      541 atatctagtg aatctcctgt taaatcttct ctattttctg aatagaaagt tagatataga
      601 ttttgaccca cgctatattt tggctcagtg atcatttcta tttctcctgt ttcgagaatg
      661 aagtaattaa aatcatcagg tagctcatcc tcgcctacgg agtcttgaaa gagtttgagg
      721 ccaaaatgct cttctaagga agttttgata gcagaaattt gcttatttaa acgttctttt
      781 tctttagaat tatcaatcac catattcacc cacactttca agataaaaat agatataaaa
      841 attatcgtaa tcggcataga taacgttgta acgcatacta tcgattacga taaaatattg
      901 atctttatta aatttcttgg cgattggatg aaatggagtc tttactttct tagttaattt
      961 cgatcccatc gcatccatag ctgttatatc actatctctc atggaaagat ttctaaattt
     1021 taaagaagtg atttctgtat cttctacacc aatctttttt cctagttcat ttcttttggt
     1081 agtttgcgtc aaaatcttta accaaccatc gttgaatgtt tcttcgagtc tacgattatt
     1141 cgccattcac atcacctgca atatattctt gtagcgcata atgttgaatg aaacctaata
     1201 actcactagc gaaattttgt tcaaactcat ctaaagcacg attccagtcg tatctacatc
     1261 tttcgattag caatccgtat tctaagcttt caggagaaaa agaaagtgtt gtactcactt
     1321 tactttgaag ataaacagca tttttagcta tcatcttttt aattgactca tcttcttcgt
     1381 tccaggtaac gtaaatatta tccttcacag ctattagcaa ttcttcagtc acttgttcag
     1441 gcgtcatcta accaccgcct taattgcttt aacatatgcg taagagcatt ttttcttgtt
     1501 tacaaatgat aaatcttcat caaaaggcgt agaagtcacg tatctccctt tgaaaaataa
     1561 atcttcatcg tttgttgtta ctccagcatt gtgtaagatt tttacttctt taactttttc
     1621 tattggatca gtagcaaaac aaaagtctaa ttcctcgtga actttaggac caatattgaa
     1681 atacatcatg ttccaaagct gtgcccacat ctcggctgtc cagatttgta tatttgtttt
     1741 ttgccctcta aggtagcgat atagccgatt agaatccaga taaacctttt tccaataatt
     1801 cgctttaggg cggttaataa cccactgtgc gcctcctgaa ttagtgttta tagtttccaa
     1861 agattctact gtaacattta caatgtttgc catatctttt agaatatttt ctccgttttc
     1921 acagcttcta atataatcaa gacttagata actacagcag tcgctacaat accaaacatc
     1981 atctttagaa ggcaatttgc gcaaattaat tcttttattg aaaatgacat ccgaatcgat
     2041 atagaaatat cggtcgtcct cacgcgaatg atcttcttct aaatatttcc accataaata
     2101 tggtttaatc gaaggaatat actctttgtc gtcccgcaga tcatcgtaca catgaacttc
     2161 aacaccatat tccttctcaa aaaaaatagg aatctgatca tcgtgtctgc tgaaaagcaa
     2221 tacgatatct ttgattccta gtttcttcag attagttaaa caaacttcaa gctcccattt
     2281 aaaccgattg attgccggct gacaaagaat atacttcatt ctgatcacct acgcttgtgt
     2341 tgtagttgtt gtggttgttg gttttgtagt tgtagtagta gttcccaaag cgctaatatc
     2401 taatacaatg aaactatcgt tacgtttagg ttgaccgttt gcatattgtt tagctagata
     2461 aatgcgttcg tcttcaacaa aatggtattc atctgaagct tcaattttta gtgtagatcc
     2521 tacacccatg aagtaatctg aggctacccc aataactgct tttccttctg gcacagccgt
     2581 tgactgcaaa tctgaaactg gtactggcaa tacttgtacg tattctccat tagcagttag
     2641 tacagtctta gctgggaata ctttagacca gtaatcagtt ggattcacaa ttaggaccac
     2701 atcagaagga ttcacattac gataaatcgg atcattcaca ccttcgatat tgaattttga
     2761 tagtcgcgcc atcaaaccgc ccatagttac agcatctaaa gctgtaatag gttctgcttt
     2821 tttttcagca tattctccgc tagtttgttt gctcatgtca cgcatcattc cgactggcat
     2881 atctttacca gtaccatcaa caattgcttg ttctaatgca attctcaatg attctactaa
     2941 aacagtacgg acataacgat ctaaccatac tggacctaaa tcaagcattg ccttacatac
     3001 aggaatataa cctgatagct tgaactgctt catgttaatt acatcaaagc cattatctaa
     3061 aactttttta acagcttcgc aaagtttacc ccaccatgct ggattgactc cacgtgacac
     3121 aatccattct gttacaccag ttgtgttaac aaaagtaatt ttttgcaata gtggatgaga
     3181 ttgttctaaa tcttcaaata cacgttcaaa tacagtagct ggcactaatt cttcgacccc
     3241 tgcaaaacct tcgtttttca ctacttcgtt atagaatttt gtttcttgtg tagttaatac
     3301 acgctgacca cggttcatta atactaattg atcttgattt tttgctgttg cttcttctaa
     3361 aattttatcc tgaatttcct tagataagct taccatagct gcgctaaaag attcttcgtt
     3421 accatcttta aaagctttca tcaattggtc gcttgcagct gttacacctt ttaaattttt
     3481 aactgtcatt attttacatc tccttgtcca aatgttttat ttaatgctgc tgtaaatgca
     3541 gcaatttttt ctgctctttt ttctttaacg tcattcaaaa tttcttcaac gctttgttct
     3601 tttttagctt cagtacctga gctattttct gcatcgataa tttcatcgac caatccataa
     3661 ctcaaagctg tttctgcatc cataaacgat tctttttcaa gaagttcttg caatgcttca
     3721 tctgtgccat tgaatcgtgt tttatatgaa gcctttaccg atttatcaat tgattccagt
     3781 tggtcagcaa tcgtacggaa gtcatcgaca tttccttctc cgtatgtgga agcgcggtga
     3841 atcatcaatt gtgcattgtt gtagattttt atagtatcgc cagccattgc gataattgaa
     3901 gcagcactag cggctaagcc gttaatcaca acgttaactt ttgctttatt tgacttaagt
     3961 aagttcccaa tagcaatccc ttgaaatacg tctccaccgt ttgaattaat tactacttca
     4021 atttcttctt gatcacctag actatccaaa atatttttga ttcccttgtc agtattccct
     4081 tcaaagaacc aactagaacc aataaatccc tgaataaaaa tttgcggtac tgcgccttca
     4141 ttctttactg ctagaaatgt tttcattgtc gtcattcgcc tcacctcctt tcgatacttg
     4201 ttgattgttt ttagttataa atatttcatc tgccatcgcc ttatcagagc gatcatttcc
     4261 aacgcgttct cttccttcgt tgattgtaaa tactccattt ctaatgccta catcaatagc
     4321 gtcaaccaaa tctttgaagc tagtaatctt gatcatagtt gtatccacac gtacaaaatt
     4381 ccctgacaag tattcttcta cttcatagag actagcgtta aacgcatcct gaataagttc
     4441 agcaatcggt atgatttcga acattaaaaa agcgtccact tgatccgata acccactcat
     4501 gtctcccttt agtaggtttt tcggaacgtg aaacgctgct gctgtcatct caaagatgtc
     4561 gtctattaag ttttttatat ctcttgaatt gctttggaag tttccgctga aatcttctaa
     4621 tgtgtactca ttttgtaatt gaaataccgc acctgcatta tcagcttcca taaaagcctt
     4681 aaattgtgat gtcatcattt tattgatttg atcttgtgtt gtattgtctt gcggtcggaa
     4741 taaattccct ttcagtacgt atctacgagc gttagagcgc ttgtaaacat tcatggcact
     4801 agaaatgagt ttcccatacg cttgataata cgcatcgact agttgcctaa tttgttgatc
     4861 tgcgtatttt atatagataa catcactttc tagaaattct ctatcaagga ctatgttgtt
     4921 aatttgcact tgagaaaaca catcatcttt caatgcatat tctgtgacat cccaactatc
     4981 cgcaataaat atttcgctag aattattaga cggagaaacg atcaatactt cattgtagaa
     5041 tattaatctc ctgatcagtt tttttctaaa ttctgttgca ttatttttct tattaggagc
     5101 tacattcagc ctatagtaaa gatcattctt tttatttttt ccatcttcat atgacttgaa
     5161 ttccgcttta ctcatcgcat ttgcaatcaa atcaatacaa gtttcaatcg caaattttcg
     5221 atacacaaaa tcaacttgca atttacaaaa gtattcttct aaaggaaccg ttgctttttt
     5281 tgtgaagtat cctaccgcct tttgaaaaat ccccactttc tcacctcctt tcaagttaga
     5341 atactagagg agtaaatcca gttcctgtat tttctactga gctatttgtg actgttacag
     5401 gagcagaatc ataaatatca tctaaaaaat tcaaaccatg aaggaatgaa aaaaagccat
     5461 ccgtttttct agtttcaggt tctatttttt catagcgtat atttccatta gaaatatgct
     5521 cttcatatac attcatgcaa taccaacgca taatcgcatc gtcaccaaaa aataaacgtt
     5581 gattaataaa aaggtcatca accagatctt ttaacatacc atgtgtaaca gatccgcttc
     5641 gaacaatttc cacagtaaaa cctgcttctt ctaaagcggg cttcaatatt tttgcacggt
     5701 acatatccat agcgattttt ttaatataat atttattact catttcaaga aaccaaccta
     5761 caatataatc agcttctata ttttttccat gaacgatctg tgattttcct tgatctatag
     5821 aaatatctat aacctctcgt ttgatgtttt gtaatcgaag ggctgattcg tggataaaag
     5881 tatgttgtgt aaaataaaca tctttatcgt attttcctag caacccaacg ctggcaaaat
     5941 ctcgtctatc agcaaaatcg actgttccta tcacttcatc cattttttca ggaaattctt
     6001 tttctttcgt atgcagaaca tcatcatatg aagcaacagc aaatcgtgta tcttccatag
     6061 gtctgttcat tcgtttggtc atgaacgtaa gtcttaaacc agcattacgt tgcatttgag
     6121 agtattcttg aaacattttc cgttttaaat ctgcattgta attaatagtt ggacaagctt
     6181 tttcccacat gtcgggatca tcaacttcat tatcgttatc caagcgacaa ataaatggaa
     6241 acaaactaga aaattctgct ccatccttgt caattccaag ttctccagaa agaatcattt
     6301 ttgattcttc tataatgtca tcaagcggac caccacgaac atgaccatta gttgtatcat
     6361 aaaattctct ataatctcga attttaccac caccagaagt agccacattt atcattgaat
     6421 aatcttcatt ttcgtgaatt tcatcaaagc ggtttgcacc tggtcgcttc ccatcttttg
     6481 ttctagcatt tgccgtgtta taacgaagtt tgctgtttgt agcgatattt tgaataactt
     6541 ccttcgtagc tttaaatact tttttatcta aatcaggatg atctttaatt actttaaata
     6601 catcatcaaa actagtcttt gcttggcttt cattattggc atagatatca atatcataat
     6661 ttttaatacc gtgtttagcg gttagtagaa agaagttgtt ccaagaagca aaaccagttt
     6721 taccattacc acgtcccatt aatgaaagat atctattgaa cactagtgtt ttatcttttt
     6781 tccatcgaac accataaata aaacattgta gaaatttttc ccacggaatt aattcgaatg
     6841 gaaagtattg tgctggtata ttgattgaat cctctaccat ctgcttatcg aagtaaatat
     6901 cttctctagt aaagactctt tcttctagat aattttttag caataattgc tctttgcata
     6961 ccttgatagt gccttcttct atagctttga accaattttc aatatgctta taactcagga
     7021 attgattcat ttgcttcacc taccaattca ggagtaatgg caagtttatc caacatcaat
     7081 cccatttgtt tgttgacaga aacaagcaac gctactgatt cattcttttt accattctcc
     7141 agtctaatgc cgttctcgga tatatcttct tccagtgata tcgccgtttc ccataaactg
     7201 atataacgat caacattatc taagaatggc tcaatatttg ttttctgact ttccaattgg
     7261 cttattaaag agcggcgtaa tttttctctg tagcgatttt gagacaattc gtttttaaac
     7321 attttagccc tcctttcatg ataaagttcg aaaaaatctc ttttcctgac agccccctcc
     7381 gtttcatcac ccccaaaaaa tttgcgattt attttaaggg ggggttatct caccatcgga
     7441 atgaaagctt cagcgaagtc aatgtaataa ttaatctctt caatactata tccaaaaaca
     7501 ttttttattc tttcgacgtt attatcttta ttcaacgctt ctcttacttg attcactttg
     7561 tatttactac aacagttatc tgataacaga tccctaatac ctacatagcg aacgtatatc
     7621 aaacgtttaa ttaatccctg agtataagat gaatactctt caatcttttc tgtatcatac
     7681 tctctgccat tatcattgat gatcatgcac ttaccacctt tcacttgcat cgaagttagc
     7741 aaagctttct atcttcttct cttgtttatc taatgctgta agatatctgc catgaacttc
     7801 attatgatgt tcaacacata aacaaataag attatctaaa tctaaagcta agtcaggtct
     7861 atccttgact tcctttatat gatgaacgtt ctctactcta tgatacttac ctagtcttct
     7921 acactcttgg cattcatagt gatctcgttt catcgctttc tctctaagcc tgcgccattt
     7981 aggagactga tagaacttaa ccaaacgatc ttctcttgtc aactgtaata gccatctata
     8041 gaattcctcg gtcatgttcc gtctcctttc gcaatcttat ttaatgctta gctattcttt
     8101 tgccatacaa tggaataact tcattgtttt cctttcgttt atatgtatcg ctctttattg
     8161 gtcttctata ctcatatact ttttcgccat tacctttatg aacagtgatt acttcatact
     8221 tctgttctaa gtattgtggt ctatacattg ttgtttcctc ctttgtgcaa aaataaaaag
     8281 accactcaaa gagtgatcta atatgtaata gtaacttgca cgatgcacaa aacgcgtacg
     8341 aaattgcgca cccctatatt tttaaaccgc cgatgcctcg gttgcttaaa gtcgctggag
     8401 tgggattgca ccactcacga gaacttacca ggctctcacg aggctactcg ccatttaccg
     8461 ctgcgtcttc tacttccgcc acagtgaccg aagcttggtg gtgtacagat agcacactta
     8521 ctacattgcc gtacgtagca ccgtatagct tcttaacgat cttttttcgg tagtcgtaac
     8581 cgtcataggc atttaatgtc
//
LOCUS       DACDHJ010000101         8434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDHJ010000101 DACDHJ010000000
VERSION     DACDHJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.103"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(123..800)
                     /gene="nth"
                     /locus_tag="IV223_001746"
     CDS             complement(123..800)
                     /gene="nth"
                     /locus_tag="IV223_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8712031.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(807..1505)
                     /locus_tag="IV223_001747"
     CDS             complement(807..1505)
                     /locus_tag="IV223_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8712032.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1773..2957
                     /locus_tag="IV223_001748"
     CDS             1773..2957
                     /locus_tag="IV223_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8712033.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2963..3406)
                     /locus_tag="IV223_001749"
     CDS             complement(2963..3406)
                     /locus_tag="IV223_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8712034.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3632..3919)
                     /locus_tag="IV223_001750"
     CDS             complement(3632..3919)
                     /locus_tag="IV223_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712035.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4090..5442)
                     /locus_tag="IV223_001751"
     CDS             complement(4090..5442)
                     /locus_tag="IV223_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8712036.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5715..6161)
                     /locus_tag="IV223_001752"
     CDS             complement(5715..6161)
                     /locus_tag="IV223_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8712037.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6397..7323)
                     /gene="cysK"
                     /locus_tag="IV223_001753"
     CDS             complement(6397..7323)
                     /gene="cysK"
                     /locus_tag="IV223_001753"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8712038.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7463..8350)
                     /locus_tag="IV223_001754"
     CDS             complement(7463..8350)
                     /locus_tag="IV223_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8712039.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2287 a         1739 c         1406 g         3002 t
ORIGIN      
        1 gttaccatcc tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa
       61 acagcttttc tccataataa caagataatt catacattta tttgcaatta aagaaaccac
      121 gtctacaggc gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca
      181 tcttgacata ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga
      241 tatctaccaa aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc
      301 cgcatcagtg tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt
      361 tttgagacac gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc
      421 aatacgacat ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac
      481 gttggcactt gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt
      541 gctttatttc gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct
      601 tctgctaacg catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact
      661 gatacatctg tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg
      721 tgcttgagtt ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt
      781 gttttttgtt tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc
      841 ggcaattctt cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt
      901 cgtttttggt cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc
      961 aaaatgcggt caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct
     1021 agcttcaaaa tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac
     1081 tcgatcgaag aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt
     1141 tctttttctt gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat
     1201 agatcataga tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt
     1261 tctatcttga taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt
     1321 ttgattccca tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt
     1381 gccatatgca actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa
     1441 tttttaagta aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc
     1501 aacatgattt ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac
     1561 aggtttcttt tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt
     1621 ttgtttgttc atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat
     1681 ttcgctgttc cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc
     1741 aagttaacat tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta
     1801 aagtttttgg atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg
     1861 tacttccgtt tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca
     1921 ctttgctcat gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct
     1981 taagtgatca ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg
     2041 gatatttgct ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg
     2101 aaggattgac ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac
     2161 ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc
     2221 tgggtcctat tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg
     2281 gagcactctt tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc
     2341 caataaaaaa acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac
     2401 aacaattatt caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt
     2461 tggcagctgg ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga
     2521 aggctgggtt ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat
     2581 tgttcattat gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga
     2641 tgttcagtga aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga
     2701 ttttgttttt atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat
     2761 tcaatggcca actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta
     2821 cccaaagtat acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg
     2881 ctgcagcacc atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc
     2941 actacaataa aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga
     3001 taaaacgaaa tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc
     3061 gctgtaccca tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg
     3121 tcaattgaaa atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc
     3181 tagctatcag tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg
     3241 ctaattttgt cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat
     3301 ttagagtaaa tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat
     3361 tagctaaatc cagatattct tctattagca gttgtctttt atccatttct acaccttctt
     3421 tttctttcat tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg
     3481 aaaacccaat ctgaaaccaa gaaaaaaagg ggttgacaaa gcttgtcata accccttttt
     3541 ctgattaaaa gatagtaaag agacagtcat cggtaattgt gatgattttt tgtcatcact
     3601 taaggatgag cggccttttc aaaaactgtc tttaaagatc atcatcagtt tctagcacat
     3661 atggctgacc ttcatatgtg tactctgtat tcaatgtgcc gtcattttca tctgagtaga
     3721 tgcgtctact gaaaggaaaa cgatgagaaa cattatcttt ggcgtcattt gctggcatcg
     3781 tgtaatattc ctcttgcgtt tcattcaact gagagagtac ttgatctgcc aactcatcaa
     3841 tattgatcgt taatttcttc gaagcacttt tataatcttc taaaaatgca tcttgttgga
     3901 caaaatattc tttattcatc ttctttctca cctttctttt caaaatcaaa taaagctgct
     3961 tgttctttta tgtaacagct ttatctttgc tgcttattgt gacaaaaaat tcgaagaaag
     4021 acaactaatt gtacttttca caaaaaaaga cagagggtcg tgacaaaatt gtgtcacaac
     4081 cctttttctt tattgtgcag tttcgatttc gcctagcgct gcttgtgcaa gtaggttcaa
     4141 gtaattccat gggcggtcaa aatgtggttg gaagaagaaa tctgaaagtg cgagatcttc
     4201 tacagtcatt ttattttgaa cagctaaaga catggtattt gctgattgtg taatgtcgta
     4261 tttggacatg aactgagcac ctacgatacg attcgttcct ttttcataga ctagctccat
     4321 cattactttt tcagtagttg gcatgaactc tggacgataa ttatcttcaa atacagtcgc
     4381 ttggacttct agatcgttca aagttgcgct ttctttggtc acacctgtcg aaccgatcgt
     4441 ccagccaaat aggtaaagtc ctgaagttcc ttgagtacca cgatacgcca ttttatgatc
     4501 tgtcaagtta tttcccacaa gcaaaccttg acgaacggca tttgttgcca aaggaatata
     4561 gttttgcgtg ccacttggat tgtagttgac tactgcagag tctccagctg caaaaatgtc
     4621 tggattactt gtctgcatgt attcattcac tttgattgcc ccgtttggca gtgtatctac
     4681 ttttccttcg atcagttttg tatttggacg gaagcccaca cataaaatga ccatatctgt
     4741 atcaaatgta tctgttggtg tagctacttg tttcactttt ccttcttcat ctgtgataaa
     4801 ttcagtaaca ttttcgccta gcgcaagagt aacaccgcgg tctgtcaatt ctttttctaa
     4861 gatatctgtg aatggtttat ctagatattt attcaagatg cgatctaatc catcaatcaa
     4921 tgtcacttgt tttccagatt cgacaaaagc ttctactaat tcaataccga tatatccacc
     4981 accaacaatt actacacgtt ttgcttcttt cgcttgtgca ataatttcat ttgcttgatt
     5041 gtagtttttg cataggagta catttttcgc atcgattcca ctaatcggtg ggataatagg
     5101 ccatgaacca gttgtcatca ctaatttatc ataagaaacc gtttctgttt ctcctgtttt
     5161 cagatctttt gccgttactg ttttattttc tgtatcgacg tctgttacat cgtgttccat
     5221 tttaactgtt gctcctaatg aagataattc ttcaggattt gagtagaaca agcctgctgg
     5281 gtcctttact actccgccga cataaagtgc gataccgcaa gataagaaag aaatattgtc
     5341 atttctttca tatactgtca cttctgcttc tggatgattt tttaaaatac tttttacagc
     5401 tgcagtacct gcatgagtac agccaattac tactactttc atttatggtc cctccgatta
     5461 acattttgtc cttcgacaaa actactataa cagaaataaa acggatacaa cagtttaatg
     5521 cttgtgaatt cacaaaggtt tgtttgtagt ttcattcctt ttcaagcata agaacccaat
     5581 atctacaaga ttcttatttt aataatattc acttttatat ctaagattgt gaataaaaga
     5641 caaaaaaata aaaaagctag cgtttttttt acacagctag ctttttttag gtataacact
     5701 tgtagaaact gtttttacat ttgcgccttt tttgctgact ctttacaatc cggacagagt
     5761 ccatacaatt ccaaccgatg ttcattaatc tcaaaatcag tcagtctgct tgcagccatt
     5821 tctatatctt caagccctgg gtaatgaaaa tcaacaatct taccgcagtt ttcacaaatt
     5881 acatgataat gacgctttga actgaaatca aatcggctag aagcatcgcc gtacttcatt
     5941 tcttgaacaa atccaatcga agtaaataag cgaaggttat tgtaaacagt cgcaacactc
     6001 atatttggaa accgatgttc aagtgcttta taaatctcat cagccgttgg atgggaatga
     6061 tgttcaatca aatattctaa aaccgcgtac cgttgaggtg tgattcgaat gttctctttt
     6121 tttaactttt cgataacctc ttcgactaac ttattttcca tgtaaacacc ccacgttgtt
     6181 attctcgtct tatcatacta tttttacatt ttaaaatcca cttaacatga aaagaaaatt
     6241 caattttatt gtattttaat gaaaaaagac gtaaaacagt cgcgttcact attctacgtc
     6301 tctattcaaa taatttttct catcataaat ttttgtgtgt tcttttcatc tttaaaagtt
     6361 ttttacataa acctatctgg agaaaaacca agtgccttat atctcttggt aaagggctgt
     6421 agataaataa cgttcaccat tgtcgggaac cacagctaat accttcttgc cttttcctaa
     6481 ttcttttgcg acttttagcg cggcagcaat tgcagcgcca gatgagatgc ctactaaaat
     6541 cccttctttt cgcccaactt ctctagctgt ttccatcgct tcgtctccac taatagaaag
     6601 gactttatca tacacagaag tatccagtgt tttaggaaca aaacctgtac cgattccttg
     6661 gattttgtga ggtcccgctt ctttcccttc taaaatagca gattctgctg gttctacccc
     6721 gattaattcg attttcggat acacacgttt taattctcgg ccagcgccag tgatcgttcc
     6781 tcctgtaccg atacctgcaa caaacgcgtc caagccattt actccaaatg cttgatgtat
     6841 ctcaggacca gtcgtcttct catgcacaag aggatttgct tcattttcaa attgtaatgg
     6901 taaaaagtag ccgttttctt ttgccaaacg ttctgcttct ctgattgatc cagaaatacc
     6961 atccgcacca ggagtcaaaa tcaactctgc gccgtaccct ttcatcaata aacgtctttc
     7021 gatactcatc gtctcaggca tcacgatgat tactttatat ccttttgcaa ctcctaccat
     7081 cgacaatccg attcctgtat ttccagaagt aggttcaatg atagtatcac ctggtttcag
     7141 tagaccatcg tgttctgctt tttctatcat acttaaagca atacgatctt ttacacttcc
     7201 tcctggatta aaaaactcaa gctttacaaa aacatctgct gaatcttctg gtacgatttt
     7261 atttaatttg acgattggcg ttttgccaat caattcggtg actgagttat agatagctgt
     7321 catcttcaat tcctccttgt ctttgctctt cttacttatc ataatacaat ctgttcattt
     7381 atggcgagtg attcgtcttt gactagcaaa aaaaagacaa agatatacca tatatggtaa
     7441 tttctttgcc tttttatcta aatcaaacgt ttagttcttg accaactaat aaagaataac
     7501 tggacataga catgccattt ttcgccatta gatcatcaac agacatgcca aatttttgtg
     7561 caatatccca tagcgtatca cctgacacga ctgtatatgt ctgaccatta tttgtattta
     7621 catctgttgt tgaagatgat gacgttgaag tcgtcgtagt agtcgttgtt gttgtacttg
     7681 tggttgttgt agccgaacct gttgatggcg tatcaaaacg ggttaaatta taagtattaa
     7741 tcaatgagtt caatttggcc ccataatttg gatcggtagc ataacgacct gtcaagtacg
     7801 ctgttgcatc ataaaaactt gttgtattgc ttttccacac gccagcataa tgataatcgc
     7861 ccgtggaaaa actggttgaa cgaagaacat tcgcatggtc ttggaaggat tcccaataag
     7921 aagaatattt tcggaatggc tcattcatcg ttacccattg tccgtttaca tattctttgg
     7981 ttgccatata aacgacctgt ccgttatagc ttcctttcac accgaataga ttataatttg
     8041 gcgcactagc taatccggaa gtgccatatg cactttccaa caacgcttga gcgatcatga
     8101 ctgaagcata caagtcatta cttgcagcaa cttctgacgc agaccagccg atttgatcga
     8161 taaacgcttg aggatcagcg actgtttgag atccttgttc atctgcatga acatgttgcg
     8221 gtaccgctgt aaccatcatc ggcccaacta aaagactcgt tccaattaaa ggagcaatcg
     8281 cttttttatt gatcaccatt tttcttttag gtctatggtg ccgggcactg cgggtcttca
     8341 gctcttgcat tcaaattcct cctcaaaata aaaaaactta ataaaaagtt ctcttcttaa
     8401 aacaaactta attaattaaa ttatactttt aatt
//
LOCUS       DACDHJ010000102         8391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDHJ010000102 DACDHJ010000000
VERSION     DACDHJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.104"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            113..583
                     /locus_tag="IV223_001755"
     CDS             113..583
                     /locus_tag="IV223_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8712040.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            597..1370
                     /locus_tag="IV223_001756"
     CDS             597..1370
                     /locus_tag="IV223_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8712041.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            1384..2196
                     /locus_tag="IV223_001757"
     CDS             1384..2196
                     /locus_tag="IV223_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8712042.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            2250..3215
                     /locus_tag="IV223_001758"
     CDS             2250..3215
                     /locus_tag="IV223_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8712043.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            3331..6054
                     /locus_tag="IV223_001759"
     CDS             3331..6054
                     /locus_tag="IV223_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712044.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELYITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            6067..6699
                     /locus_tag="IV223_001760"
     CDS             6067..6699
                     /locus_tag="IV223_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8712045.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            7598..>8391
                     /locus_tag="IV223_001761"
     CDS             7598..>8391
                     /locus_tag="IV223_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8712046.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         2844 a         1266 c         1658 g         2623 t
ORIGIN      
        1 ataatggaaa aattggggaa agtgagctag aaaagcttat taaagagagt caacaatcag
       61 tgattgattg tagaaaatta ttagtagaac aacttactgg aggagatgac ctatgattaa
      121 gttagtacgt attgaccatc gtttgttgca cggacaggta gtattttctt ggagtaaatc
      181 attaaatatc aatcgtatta ttattgccaa taaagaggca gcgaatgatg aattcaaaaa
      241 aatgtctttg aatttatcta aaccgactgg cattaaatta acaatttttt cagttgaaca
      301 gttactagaa aaaatgccta aaatcgaaac acttaacgaa aatatcatgc ttatttttgg
      361 taatacaaaa gaaattcgtc aattttgtga agcctatcct aaagtacaag aaatcaacta
      421 tggagggata atcaaaaaag aaggttccaa gcaatttagt aatgccattt tcttgaatga
      481 atctgagcaa gaagatgcca aaaaattaaa agctcttggc attaaacaat ttattcaaca
      541 ggtaccaact tctaaaaaag aagatttgaa tacaatgtta taaaggagga atgaaaatgc
      601 tcgttcagtc aattggttta gggttaatcg gtgttttttg tattttagac tcaaggcttt
      661 taggaagaat gaattttgaa cgaccgttga ttgtcagtac gcttgttggt ttggtacttg
      721 gagacatgga aaaaggatta attgttggag cctctttgga attgatgtcc ttaggattag
      781 taaatatcgg agctgctgca ccacctgata tgaatatggc atcaattatt gctacagcct
      841 ttgccatttt atcaaatgcg aatgcagaag cagcattaac gattgccatt ccaatttcag
      901 tgttaggtca aatactaggt atcttaatgc ggacgatttt gtcaaatttg actcacgttg
      961 cggatcgctt tatcgaacaa gggaaattta gccaagcaag gagaattcat atactttatg
     1021 gctcgggatt atatgctctc atgtatttta ttcctatttt tctagcaatt tattttggaa
     1081 cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg gctaacaaat ggcttaacgg
     1141 ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt attacaaacg atgttaacga
     1201 aaaaaatggt gccattttta ttacttggat ttttaattac tgcttattct ggattgggaa
     1261 tcattggtat tgcagccttc gcatgtattg ttgcgtttgt catggttcaa gttcaaggta
     1321 aaaactcaaa tggtaaccaa aatgagccag atccattaga tgacttataa ggaggtagat
     1381 taagtgaaag agaaagatca aaatgcatgg aaatatcggc agttcttatg gcgttcatgg
     1441 gcaattcaag cctcatggaa ctatgaacga caaatgaaca tgggttttat gtacggaatt
     1501 gcaccaattc tagataacat ttacaagaat ccagaagatg aagcaatgaa gaaagaagct
     1561 tacgagcggc atatgatgta ctacaactgt acccctcaaa caagtgcatt tgtgttaggg
     1621 ttaactgctt ctatggagga gcaatactac gaagaccaag aaaactttaa tccggaatca
     1681 atcagtgcag tgaagacttc tctgatggga ccgctatctg gtgttggtga ttctttcttt
     1741 caaggaacga ttcgggtatt agcctttgga ctaggaatta atttagccca acaaggtagt
     1801 attttaggtc caattctagc aatgttaatt tcctttgtac cttccttttt agttacttac
     1861 tatggtggga aaattggcta taacatgggg aataaatact tgtctaaact gtatcaagaa
     1921 ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg ggttgatggt cattggttcg
     1981 atgattgcca gcatgatcgg gattacaaca ccaattcaaa tgggtaaagc ttttgtctta
     2041 caagatgtat tagatagtat tatgccacaa tttttaccat taggattaac cttcttcatg
     2101 tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt tggcaatctg tttagtcgga
     2161 ggaattattt ttagtttgtt aggtatctta caataaggga ataaccaata aatttggttg
     2221 aataatataa ataaggagga attcaattga tgaattcaaa agaaattgtt aaagaaatcg
     2281 tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg tggcgcttca aaagcggacc
     2341 tttatcctgc aaagtatttt ctagaccaaa atgcgaagca gttacggatt agtcattaca
     2401 cagccagcga atttaactat gcaacaccag catctgtaga tgagtcaact gtcgttattt
     2461 cagcttcttt aggaggtacg actccagaaa cagttgaagc taacgcagta gctaaagaaa
     2521 aaggtgctac agttattagt ctaacacgaa gcattgactc accgttaaca aaagatgcag
     2581 attatgtaat ttatcatcga tttgcagaga actatgctgc taaattagaa aaaacaggct
     2641 actgtttaga attggcagta gaacttttac atcaagtaga aggttatgac cattatgaag
     2701 caatgcaaga aggatttgct actatctatg atcttgccca aaaaagtgca gaatcagctc
     2761 gtaaagatgc gaagaaattt gctgaacgct ttaaagatga aaaagttatt tatgtaatga
     2821 gtagtggtgc tacacatgaa gtagcatact caacatcaat ttgcttgatg atggaaatgc
     2881 aatggattaa ttctggtacc ttccattcag gagaattttt ccacggacca ttcgaaattg
     2941 tggataaaga cgtaccgttt atattattaa tgaatgatgg aaaaactaga aatatagatg
     3001 ctcgagcatt aactttcctt gaacgctttg atacgaaaac agaagttgta gatgccttgg
     3061 attatggctt gtcttcacat attgctaaag aagtcttaga ctactttaac ccattcgtga
     3121 ttactgcagt atttagagtt tatgcagaag aattgtcaga agcaagagaa catccattaa
     3181 ctaagagacg ttatatgtgg aaactaactt attaaaaaat agatcaaact aaataaggtg
     3241 gcttgagaaa ttaagccacc ttgttttctt caatgtgatt ttatacatat ggtaaaataa
     3301 ctagtacata ttgtgaaagc aggtggcaag atgcagcgat taaatgacta tctttatcaa
     3361 ctaacattgc ttttgaaatc tacgccttca gataattatt cagctcgcag tatttcgcaa
     3421 gctttacagt tgaatagaag tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg
     3481 cttttcataa aagtaaaaag ttacccggtt ttatttttac atagaacagc tcttgaagaa
     3541 ctatatatca cactgaataa ctccgaaata gaatcaattg aaagcttgtt aacaatttct
     3601 caaaaacccg cattagatca agtaattggc tctaaaggaa gtttgaaaga agcaattgat
     3661 caaattaaaa cggcagtctt atatcctgga aaagggttgc ctttattatt gatcggcgct
     3721 agtggatcag ggaaaacctt tctagcgaat aaaatatatg aatatgcagt agaagaaaag
     3781 gtcattcaca catcggcacc tttcatagat tataattgtg cacaatatgt gagcaatcca
     3841 gagttactat cctctgcttt atttggctac acgaaaggag catttaccgg agcaagccag
     3901 gagcatactg gtttgctaga aaaagccgat ggaggagtat tgtttttaga tgaagtgcat
     3961 cgcttatctg aagaagggca agagaaatta tttacattta tggatacggg tgaattctct
     4021 cctatggggg ataatagtat tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca
     4081 gaaaatatat atacgacttt cttaccaact tttcttagga gattgccagt tattgttaat
     4141 ctacctagat ttcaacaacg tccgtcgttt gaacgtctgt cattgattga tgaatttttt
     4201 gtttctgaga gtaaaatttt agctaaggaa ctatctgttt ctgatgcatt gattcatttc
     4261 ttaatgaata gtgattttga aggtaacgtg gggaatatta aaaatattat caagtatgct
     4321 tgcggaaacg catatatcca ccagaagagt cagcaaacaa ttcaagttaa attattggat
     4381 atgcctttag agtacagtta taaattcaag gagcaactaa ataaacccag aaagaaaaga
     4441 gcagatcgaa tctactatcc agaagaaaca cgacaagtgc aactagaaag aaacaatcag
     4501 ctttttgttc aattttttga ccaattactt actggattct ctgaagtgat tgaaaataac
     4561 aaggatatta aactattttt agaagaaatg gttaccaagg taacacaagt aatggatacg
     4621 cttatttttc aagaggatta cgaaaaagag cagtctttat acgccattct aaactatcat
     4681 attcgtcaaa gtattgattt tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg
     4741 gtagtcgctt tggccaacta tctatatgtc aaggataatc aagtgatttt tgaaaatagt
     4801 cttcaagaaa agaaaaccaa agatcagctt aacgaatttt ttgctaatgt tcttgaaacc
     4861 ccttattggt atgcaaaaaa aatcttaagc cttttatctg agtgtttaga tcaacctgtg
     4921 tatgaagagg acattttctt tattacgcta tatttttata gtttgaatgt cacgaatgtt
     4981 aaaacaggta ttaagagtat tgtgctagct catggttatt ctacagctag tagtatggcg
     5041 aatgttgcca accgtatgct gaataagaac gtttttcaag cttatgatat gcctttagat
     5101 attacattag agaaaattga agcaaaattg attcaattcc ttggagatta tcgaacagat
     5161 tcaggcttga ttttattggt agatatgggt tcactaaacc aactaggcgg tttattagtg
     5221 aatcatctaa atggaccact gttaatcatt gatcatatca atacaccgtt attattggaa
     5281 gtcggccaac aagtattaaa caataaaagt atcaccgaaa taggagcaga aattgaaaca
     5341 agtcttactt tcaaaaagca gttactactt cccaagaaaa agaaaaagaa agccatcatt
     5401 acttgttgtt atacagggat tggtagtgcg acccagattc aggagatttt aacgaaatgc
     5461 ttgaaagatg cagttagaga attagtaatt attccgtatg attacaaaaa gctgttagaa
     5521 aacaagaatt ttgagactcc ttttcaatta tacgatgtca tcatgattgt aggtacagaa
     5581 gatcccaaaa ttaatcagat tccatatatt ggattagatc gcctgattaa tggggaagca
     5641 attggcgaat ttgttcatta cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa
     5701 aaagaattaa ttttcaattt ttcaattagt aaaattgtcg aaaatttaac cattttggat
     5761 ccagataagg tgctatcttt tgtacaaaaa gcaattaaag aattatcgaa gttattaaat
     5821 tttgattttt ctaataatca gctgtttttg ttgtaccttc actgtagttg tatgattgaa
     5881 cgaattgtac gtaaagaaag tgtggatgag caagaggaca ttgaagaata tatgcaagca
     5941 aatggagaaa taatggataa aattcatagc gctttttttg agattgaaag ggaatacaca
     6001 attgaaatac cagatttaga gttgcgatta ttgagcgata ttgttcaagg ataggagtga
     6061 attcgaatga caatgggagt aatttttgat atggatgggg tactcataga cagcgagaca
     6121 ttttattttg agagaagaat gaacttcttt caagaactgg agatagatcc aggatcaaac
     6181 cagattgatg attatgtggg gaaaacagaa gctgcaatat gggaaacatt agttcctgag
     6241 gacaaatcat taagagagga actttatccg aaatatgtag attatcgaaa aacgcatcca
     6301 attgatttcg ctaaagattt acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag
     6361 cgggatatac cattggcact ggcatcttct tctccagttg ttgagataga gagtatgctc
     6421 attcaatgtg aacttactga atatttttcc tttgttataa gtggtgagtc attaaaacaa
     6481 agcaaaccac atcccgaaat atatttaaaa gcgaaacagc aattagactg tgacttcttt
     6541 attgcagtag aggattcaac gattggtatt aaatcagcaa aggcagcaaa tatctatacg
     6601 gtggcactaa aacaaccaat gcaaatcgat cagagtgaag cagacataca aattgaaagt
     6661 ctacaagaac tggaacaaat tgttttagaa aaaggttagt gaattaatgg aaataaggct
     6721 atatatctgt gctagctatt ttgactaacc aaaaaaactt gctataattc aggtgtagta
     6781 aaaggtgctg gtaacacctt ttacgaaaat gcttcggcag tgataatggg attggctttt
     6841 taagtttttt attttgttcg caatccctaa ggtcatgtga atgatcctag ggattgctag
     6901 aatattttgc aacattctat ctatttcaga acgtaacctt ccccagttca ttgggattca
     6961 ttagcataca accaacattt caaaaagaaa ttgcactaca ccattattag ctattttgac
     7021 taaccccaaa aaacttgcta taattcaggt gtaggtaaaa ggtgctggta tcacctttta
     7081 cgaaactgct tcggcagtgg taatgagtgt ggcttaaagg actagtcctt taagcttttt
     7141 actttgttcg caatctctaa gatcaagtta atgatcgata agattgctag aatattttgc
     7201 aacattctat ttcagaacgt aacctttccc attccactgt gattcattgg caaccctcct
     7261 cacaaccaac gtttcaaaag aaattgtaaa gttgttgggg taccacgacc gaacaatact
     7321 acacctgaat gatagtaagt tgtctcatta agtatagcat atcctctttt tgggtaaata
     7381 gaaatcggtt acatttttta tccaagacca agttttttca tttggtctta tttgctatac
     7441 ccaaacaatt ccgcagtata gtcgacggta ttgattttat caggtgagta atagaaactt
     7501 gttttccttc cccaaaacat tacctataaa ggagaaaaaa tggtatcgta attatatagg
     7561 tataggatga aatttttttc taagaggagg gttgttgatg aaaaatttac agttaaaatt
     7621 tattgttgat ccatcgattg tacgctggtt tcggattatc aatcaatttg agcgcaatca
     7681 acttgccaca aaaggagaat tagcaaagag taatcatgta tctgaacgaa ccattcaaac
     7741 agatgtcaat aaaatgaagg actacttttc tgaaagtgtt caatttgttt caacgaaaag
     7801 tgggtattct tttcagaagg aaagaccggt tctttttctc aagcagaagg agcaactact
     7861 ggagaacgaa attttatttg agttgttagg gaatgtcttt tatggcgaat tggaagatat
     7921 gacggagcta attgatcgtt ttcatttatc tgaagcgact tttcgtcggt atttgaagca
     7981 gattcaacca gttttggaag aatacggctt agaattgagt ctttatcctc ttgatttaaa
     8041 aggagaagag gcaaatattc gcaagttttt taaagacttc tattatgaag gggagatgac
     8101 gccccatact cttgttccac caagggatct tcatgaactt gtgcaagcta cattctatga
     8161 taaatttgag tatttctcga ttggaacagg gacttctcct gctgaatttt attatagttt
     8221 gtatattgcg attgagcgtg tgagacaagg aaaaacgata gccattcctc aaggtctgat
     8281 tgagcgagta attgctagtg agaattttcg cttaatgtat tccttgaaag aagcgattca
     8341 aacaaattat caagttgctt tatcagaaga ggagttttgt tggttgtatt t
//
LOCUS       DACDHJ010000103         8390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDHJ010000103 DACDHJ010000000
VERSION     DACDHJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.105"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(41..1447)
                     /gene="lpdA"
                     /locus_tag="IV223_001762"
     CDS             complement(41..1447)
                     /gene="lpdA"
                     /locus_tag="IV223_001762"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8712047.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            complement(1455..3098)
                     /locus_tag="IV223_001763"
     CDS             complement(1455..3098)
                     /locus_tag="IV223_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8712048.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            complement(3123..4100)
                     /locus_tag="IV223_001764"
     CDS             complement(3123..4100)
                     /locus_tag="IV223_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8712049.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(4103..5212)
                     /gene="pdhA"
                     /locus_tag="IV223_001765"
     CDS             complement(4103..5212)
                     /gene="pdhA"
                     /locus_tag="IV223_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8712050.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(5673..7097)
                     /locus_tag="IV223_001766"
     CDS             complement(5673..7097)
                     /locus_tag="IV223_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8712051.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(7361..8338)
                     /gene="guaC"
                     /locus_tag="IV223_001767"
     CDS             complement(7361..8338)
                     /gene="guaC"
                     /locus_tag="IV223_001767"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8712052.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         2190 a         1878 c         1485 g         2837 t
ORIGIN      
        1 caactgattt ttcagttgcg tttcgcctgt attattcaaa tcaaatatgg attggtaaac
       61 ctaatgctaa ttccgaagca tccatgacga tttcgccaag agatggatga ggatgaatcg
      121 tcaaggcgat atcttcagca ttcattcctg attcgattgc taaagctaat tcagagacca
      181 tatcacttgc gccgactcca ccgatttgcg cgccaataag cacgttatct tcgatcgtcg
      241 taactagacg gatgaatcct tctgttttac caagcgataa tgcccgaccg ttccctgaaa
      301 atgggaattt atatgctttt gcttcgatac cagcatcttt tgcttctttg atcgtcatac
      361 ctacagaagc caattcagga tcagtgaagg ctactgccgg cattgcttta taatctacag
      421 ctactttttt gccagagatt gcttcagcag cgatttttgc ttcataactg gctttgtgtg
      481 ctaatgccgc ccctggaacg atatcaccga tggcatagat gttaggtacg ttcgtacgtc
      541 cttgtttatc caccgtgatc aatcctcgtt ctcctacttc gacacccgct tgttctaagc
      601 ccatatcatc tgtattcgga cgacgaccta cagtgaccat cacataatca gcagtaacgg
      661 attcttcttt cccgtccact gcatatttca ctgtcacgct gtcaccgtta tcgactgctt
      721 ctttcgccat tgcattcgtt acgactgtta cgcctttttt cttgaaatca tcttctacta
      781 attttactag atctttttcg taagttggta aaatttgtgg tgagccttca agaatcgtta
      841 cttctgctcc taagtttgcg tacgctccgc ccaattcggc accaatcaca ccgccaccga
      901 tgatcacaaa tttcttaggt acttctttca atgctaatcc gccagtagaa tctaacacac
      961 gtcctccgaa tttaaatcct ggaatctcga ttggacggga acctgttgcg atgattgcgt
     1021 tattgaacga ataagtttga gctgaatctg gatgaatgac gcgtagtgtg tgatcatcta
     1081 cgaagaacgc ttctccttcg attgtttcaa ccttatgttt ttttaatagg aaaccaacac
     1141 cagaagtcaa cgtatggaca actttgtttt ctttccattc ttgtgttttt gcaaaatcta
     1201 gtttgacgtt ttcactagtt actccaaaca tttcagaatc tagtgactct tgataatggt
     1261 gtcctgcagc tatcagtgct tttgaaggaa tacagccgac attcaaacaa acaccgccaa
     1321 tatactctct ttcaatgatt gctacttttt gtcccatttc agcagcgcgg atggcagcta
     1381 catagccacc aggtcccgca ccaattacaa ctgtatctaa ttctacagca aaatctccaa
     1441 ctaccatttg tttatcatcc ttccatcatt aatagctctg gatcagctaa aagacgtttg
     1501 atattattca ttgcttgttg cgcagttgct ccatctacga tacggtgatc gaaacttaat
     1561 gacagtttca tcacacgacc gacaacgatt tcgccttcag catttacgat tggttgttgt
     1621 gcaatcgttc ctacacctaa aatagctact tctggatagt tgatgactgg tgtaaaccaa
     1681 ccgccaccta cagatccgat attgctgatc gtaattgttc cattgcgcat atcttctgca
     1741 gatagtttgc cgtcatgagc taattttgct ttctcattga tttcgtctgc aatagcaaac
     1801 attcctttgc ggtctgcatc tttcacattt ggtacatata gtccgtgatc cgtatcagtt
     1861 gcaataccga tattgtagta atgtttataa acgatttctt ggtttgcatc gtcgatagat
     1921 gcattcaata ctgggtattt cttaacagtc gctgttaaag ctttcacgac atatggcaag
     1981 aaagtaagtt tcgtaccatt tgcagcagca acttctttaa atcgtttccg attatcccat
     2041 agtttagaca cttctacttc atcatgcaat gtcacgtgtg gagctgtatg cttactgttg
     2101 accattgctt tagcgatagc tttacgtgtt ggcgtcattg ctacacgttc ttccaagtct
     2161 cctaagttgg acttgaatgg ttttgctggt gcagctggtt tgctttctgc tgcaggagct
     2221 gcttctttcg gtgctgccgc ttctggcgct tctgatttcg ctggtgcact gcttggacca
     2281 cctgccaaga agttttcgat gtcctctttt gttactctgc caccttttcc agttgcagtt
     2341 acttgtgaga tatctacatc tttttcacgg gcaaattgac gaacagatgg catagccaat
     2401 acacgtttat ttggatcggc agcttcaacg acacttgctg aacctgatgt ttccactttt
     2461 tcttttggtg cttcagctga tggcgcacta gttgacgatg cactattgtg tccaggagca
     2521 tcgatttcta ctaatacatc cccaacattt gctacagtgc cttctggaac aatcacgttt
     2581 ttaactgtcc ctgtgacagg tgacggaatt tcttctactg atttgtcatt ttgaacttct
     2641 agtaatgtat catcttcatt gatcgtatct cctggtttga cgaaccattt gacgatttca
     2701 ccttctgcga ttccttcacc gatatccggt aacttaaatt ggaagactcc ttcgcttgac
     2761 gatccagctg attctgtatc aacagtgggt tcagcagccg gtttagctgg agtttgggat
     2821 tctgcggcta caccactgtc cccttcattg tcttcgtgtc ctggtgcatc gatttctact
     2881 aatacatccc caacatttgc tacggtacct tctggaacaa tcacattttt aaccgttcct
     2941 gtaacaggtg acgggatttc ttctactgat ttgtcatttt gaacttctag taatgtatca
     3001 tcttcattga tcgtatctcc tggtttgacg aaccatttga cgatttcacc ttctgcgatt
     3061 ccttcaccga tatccggcaa tttaaattga taagccatga ctttgtttcg tcccttcttt
     3121 tcttaaaatt ccgcgatttc ttttactttg tcttcgatat cttttgcatt tggcaaccag
     3181 acattttctg cttgtccaaa tgggaagact gtatcaggtg cagaaactcg tccgattggg
     3241 gcttctaatg aaaggatagc gcgttctgag atctcagaaa ctacttgcgc ggcaacgccc
     3301 gcttgtcgtt gtgcttcttg gacaacaact acgcgaccag ttttttccac ggattgaatg
     3361 atcgtttcta catctaatgg cgcaactgta cgaagatcaa tgatttcaac agatatattt
     3421 tcttttgcaa gattgtctgc tgctttgatt gcttcacgca ccattgctcc gtatgtgata
     3481 atagatacat ctgttccctc acgagtaact gctgctttat ccaaaggaac ttcgtaagct
     3541 tcatccggca cttcttctcg gaatgagcga tatagtttca tgtgttctag gaaaacaacc
     3601 ggatcgttac ttctaataga agaaatcaat agtccttttg catcataagg gttggaagga
     3661 atcactactc gaataccagg agactgagcg atcaaacctt ctaaattatc cgaatgcaat
     3721 tctggtgtat gaacaccgcc accaaatggc gaacgaattg tgatgggtag atttctagtg
     3781 ccgctcattc gatatcttgt acgagccatt tgagctacta cttcgtccat tgcttcgaaa
     3841 ataaaaccaa agaattggat ttcaggaact ggacgaaatc cttctaacgc aagaccgaaa
     3901 ctcaatcctg cgattccaga ttcagctaaa ggagtatcga atactcggtc ttctccgaac
     3961 ttttcctgca atccttcagt tgcccggaaa actcctccgt ttttcccaac atcttcacca
     4021 aaaacaacga cattctcgtc attttctagt tcaagtgcta aggcatctgt gattgcttgg
     4081 atcattgttt tttgtgccat gattatttcg actccttcgc ttcataaatt gcaatttgtt
     4141 ctttgattga ttgtggagat acttcgaaca tgtttttcaa gaaatctgaa accttttgtt
     4201 ttggtacttt gtcggcttca gcaatagctg ctttgatttc ttctttagtt gcttcgatga
     4261 cttgttcttc tttttcttca gaccaaagtc ctttttccgt caaatattta cggaagcgga
     4321 tcaatggatc ttttttctgc caatcgtcat ctgtgtcttt tgtacggtag cgggttggat
     4381 cgtcaccaga tagtgtatgt ggtccataac gataagtcag tgtttcgatt aggactggtc
     4441 catttccact gatcgcccat tcccgtgcca ttttagatac agtatatact gcaagcggat
     4501 ccattccatc gacttgaata ccaggaatcc cagctgcgac agctttttgt gcaagtgttt
     4561 ttgctgcagt ttgtttttca cgaggtgtag agatcgcata cccgttgttt tggatataaa
     4621 atacggcatt tgcatggtaa gcgccagcga aattgattgc ttcgtaaaaa tcaccttgtg
     4681 atgaaccgcc atcaccagta taagtgaaga cgacattttt cttgtttcgt ttcttcaaac
     4741 ctaatgcaac accggctgct tgtacatatt gtgcaccgat gataatttgt ggtggtagag
     4801 ctttcaattc ttccggataa gaattacctg cagcatgtcc tcgagaccat aaaaaagctt
     4861 gagataaagg taaaccatgt tttactagtt gaggtacatc ccgataacct ggaagaagaa
     4921 catcttcttt atcaaatgcg taagcactag ccagctgact tgcctcttgt ccagcagttg
     4981 gtgcaaaaaa gcctaggcgt ccttggcggt tcagcgctgt tgaacgttgg tctaatacac
     5041 gagaccatac catatgagtc attaattcaa cgagttcatc atcggataaa tcaggtacta
     5101 aatctttgtt aactacgttt ccatcttgat caagtgcttg atatactggg aaatctgcat
     5161 tgacttcatc tagcagtgct tggaagtcga tcggtgcttt tttgtttgcc atttgtcaca
     5221 cattcctctc tcatctataa gaaaatctct tcattctgcg attgaaacag tgaataaaac
     5281 tgtatcacta tttaaccgtc ttcattaata aattatcatg gatttatgag aaatacaagc
     5341 taaaagcaca aattttcacg atgaaaggaa attatgccaa tccgcatatt aacagcatta
     5401 gtgaataaaa tcacaataag gaaagcgttt tatatttcat ctgcattttt tctattttct
     5461 aatcaaactt acaaccatct gtttctattt aaaaacgttg gtgttataca gttttgttgc
     5521 aaacatttat tttatttaca catcgtgaac ggacgtacaa ataatggttt tttcttagtt
     5581 tctttatatc gtattctttt tccgaaaaaa aagaaacagc ttgaaattta aatccaagct
     5641 gttatattct ttaatctatt tactttgtga aattattctg ttaaccactt ttctagaatt
     5701 ttcatctttg aataaaacaa cggtatttgg ttttcatcca gcctactgta attgattctg
     5761 attccattta tatcttgccc aaaaagtaga cttggtgcaa ttccaagacc catatctact
     5821 actttttctg ctagttcggg gtctagtttt ttcccatccc aagtaatcca ggcatagtaa
     5881 ccacctaaaa ttgaatgaag cttgaaaaac gattgtgaat ctccccaagc gcttatcagt
     5941 cggctttttt ctttcaactt ttttcttaca tcttgcattt tcaaatcaat tgcttcatta
     6001 aatacattag ccactgtgat ttgtgtaagg atattcatcg aaaactccat catttcttgc
     6061 gcttgagcta gttgcgagat aaaagtctca ggtcctatca cccaaccgat tttcgttgtt
     6121 tttcctaaaa ccctagataa cgaaccgacg tataacacat tatccggatc aatcgttcgt
     6181 ataggaagga cttcttcttg gtttagaaat gaaagatccg taaatacatc gtcttcaata
     6241 attggaagcc gatactgtcg acacaattct actagctttt ctcgtcgttt ttgactcatc
     6301 acttttcctg tagggttttg aaaattcggg ttgataagca ctgctttcaa tttattttta
     6361 tggatcgatt ctttcagttt ttctaagtcg atcccttctt catccatcgg tatcccaaat
     6421 aaacgcgttc ctgaagcttt gaaaagaggt aatcggtaaa aaaaagaagg tgactctaca
     6481 gctatcccct ctccagctga aagaatcgtt tgtaaaatca aaaagattgc ttgctgaccg
     6541 ccaccagtca caagaagctg gttgggggaa aatgccaatt caaatttact ttccaaatac
     6601 cgtttgattt tgttacgtaa cggagtatag cctaaaagat ccatttcttt ttcttccgct
     6661 aagatttctt caagagaata agaaggaaac tcaaaattcg gcaaaagctc atctggcatc
     6721 tcgctagcgt ataaatcaag aaacgatgtt ttttggatct gctttgcttc tcttattttt
     6781 tgctcatacc agtccgtctg ctcataacga ggagagatca tttgccgcca atcgattctt
     6841 ggaacagcga atttccccca ttcagtctga ttaacaaacc atccgctgcc acgtttgctc
     6901 gttagtaccc caagctccct aagctcttcc aatgcatgga caacggtgga acgattcaca
     6961 ccaaaaagtt gccctagttt tcgttctgat tcaagttgac ttccaggatt gatccttccg
     7021 ttttcgattt ctctcataat ctgttccata atttgacgat aaatcggtcc ttgttttttc
     7081 tctacttttt cccacataag cttctctttt ctattgtcta ttttttcttt tgattttacc
     7141 gaatttacga gaaaaaagaa aaactttttt tgcaaaacat aagggactgt gacaaaaatc
     7201 ttatcacagt cccttattct gaataatgat gttcaaaagc ttgtatctgc gataataatt
     7261 ccagccaaac aaaacatata aagaactatt tttgtttggc tgttcttatt acttaatgca
     7321 ggacaacttt ttcacaacat cctaatattt tatttatttt ttagattgtg tctccattaa
     7381 aaatcgaatt tttcacgatg acatagtcaa cttttcgaat ggcttccaga tctcttcctc
     7441 cagcataaga aatagatgat tgaaggtctt gttgcatttc cactagtgta tctttcaaag
     7501 atcctttgtg ttggatccag attttctttc cttcaacatt tcgtttttcg cctttttgga
     7561 attctgaagc agaaccaaag tattctttat acacgattcc attttcgact tttgtttcgc
     7621 caggagattc ctcgtgaccg gcaaacaatg aaccaatcat gaccattgtc gcaccaaaac
     7681 ggacagattt tgcaatatcg ccatgtgttc tgatcccacc gtcagcgatg atcggcttgc
     7741 gagctgcttt cgcacaccat ctaagagctg ccagttgcca gcctccagtc ccaaagcctg
     7801 tcttgatttt tgtaatacat acttttcctg gaccaatccc gacttttgtt gcatctgcgc
     7861 ccgcattttc caattcacgg acggcttccg gtgtaccaac attaccggca atcacaaaag
     7921 tttcaggcaa atatttttta atatgctgaa tcatatcaat aactgagtta gcatgtccat
     7981 gcgcgatgtc gatagtgata taatcaggat ttagcgattc ttctgccaac ttctctataa
     8041 aggaatattc ctctttcttg acgccaacac tgatcgaggt aatcaatcca cgctttttca
     8101 tttttttgat aaacggaatt cgagctgctt catcaaaccg atgcatgata tagaaataac
     8161 cattttccgc taagaattct gcgattgttt catcaataat cgtctgcatg ttggcaggca
     8221 ctactggcat tttgaatgta tgtttaccta atgtgaccgt tgtatcacat tcagaacggc
     8281 tatttacgat acatttatta ggaattaatt gaatatcttc gtaatcaaat actttcatta
     8341 cattatcctc ctgatgttca catccagcaa aacacgaaca ttattaattt
//
LOCUS       DACDHJ010000104         8384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDHJ010000104 DACDHJ010000000
VERSION     DACDHJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.106"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(66..284)
                     /locus_tag="IV223_001768"
                     /pseudo
     CDS             complement(66..284)
                     /locus_tag="IV223_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            525..707
                     /locus_tag="IV223_001769"
     CDS             525..707
                     /locus_tag="IV223_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712053.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            691..876
                     /locus_tag="IV223_001770"
     CDS             691..876
                     /locus_tag="IV223_001770"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP8712054.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            1119..2639
                     /locus_tag="IV223_001771"
     CDS             1119..2639
                     /locus_tag="IV223_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8712055.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2826..3398)
                     /locus_tag="IV223_001772"
     CDS             complement(2826..3398)
                     /locus_tag="IV223_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8712056.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3405..4067)
                     /gene="rpe"
                     /locus_tag="IV223_001773"
     CDS             complement(3405..4067)
                     /gene="rpe"
                     /locus_tag="IV223_001773"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8712057.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(4083..4598)
                     /locus_tag="IV223_001774"
     CDS             complement(4083..4598)
                     /locus_tag="IV223_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP8712058.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4600..4884)
                     /locus_tag="IV223_001775"
     CDS             complement(4600..4884)
                     /locus_tag="IV223_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8712059.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4918..6246)
                     /locus_tag="IV223_001776"
     CDS             complement(4918..6246)
                     /locus_tag="IV223_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8712060.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6260..6715)
                     /locus_tag="IV223_001777"
     CDS             complement(6260..6715)
                     /locus_tag="IV223_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8712061.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6718..>8384)
                     /locus_tag="IV223_001778"
     CDS             complement(6718..>8384)
                     /locus_tag="IV223_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8712062.1"
                     /translation="IIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEV
                     KLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLL
                     FNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANG
                     LIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKI
                     DLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEE
                     FDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNV
                     EISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEE
                     PSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEECLDLMISELEANSLVTEEF
                     RASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSL
                     AEEDVSEIRGVYEELYQFIGQKEMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2746 a         1569 c         1352 g         2717 t
ORIGIN      
        1 tgacactttt agttgggagc gtcaataatt ttgtgtcaat ggttcttccc tactgtagat
       61 tgtttctaat tctccatcgg ttgactcagc atttgttcga gctcgccttc ggcctgttgg
      121 aaacctttat gactgcgacc taggaatttt tggttgtaag tatcaaaaga cgagactaag
      181 aaacgttcca tcgactcttc attttggaac tgttccttgc ggtggctgta tttcttgatt
      241 tgtttattga aagattcgat taaattagtt gagtaaatgc ttcgtacgaa catagttgat
      301 ttattcacca tacttcccat tacatcgtaa aacgctatat ttaagatttc aaacaactat
      361 attaatagga agaaaacttc atttttcctc ttagttctat aaaaagtgtg gctaatttga
      421 ggcaaaatat taaattaaat gtaagcgtgt cataggatag tatctcgtac gccgattatc
      481 tttcttctat aattaaacta tcaaatatag aaggagaaaa acgaatgaat aaaaaagata
      541 ttgtaccagt tcaattttca agtgcggctc aaaaatcgca aattgacacc aagccaaaac
      601 tagcttgcag aatcaaatcg gaagataagg aaatatttat ttataatgga atggataaat
      661 atcttttgta taccttgtta aaagagttga atgccaatgc tcattgatgc aactgcattg
      721 aaacatattt ttatcgtttg tggaaaaaca gatcttcgaa aaggaattga tggactagca
      781 gctttcgtta tagaagaata tgaccttgat gtttacgatc aagcgctttt cttattttgc
      841 ggtacgaaga aagatcgctt caaggctctt tactaggatc aaaatggatt tgtattaatg
      901 tacaaaagat ttgaaagtgg gtaccttcaa tggccgcgtt ccagaaaaga agtcttactg
      961 actggccttt caatcgaaga aaaatgtacg ctaaaagaaa cgaaaaaagg tgcgatatat
     1021 tagacgaaaa ccttgttaaa tcaaggattt tgtctgtttt tcgttgtcat aaaatggtat
     1081 aatagatgta agaaaatcgc tagaatggtg gtgtaaaaat gacaaaaatg gaatctgaac
     1141 ttcttgtaca aatcaagcaa ttaacacagt tgcttgaaat ccagcaacag gaaaatactc
     1201 ttcttagaga acaaattgct gaaatgaatc gacgcttgtt tggacgaaaa aaagaaacac
     1261 cacccgtaga tgggcaaatc gatttattag acgactcaac ttttaatgaa ccagagcaca
     1321 ctggacaaga aagccaggaa cccattacgg tatcttcttt taaacgcaga aaacgcaagg
     1381 gactcaaagc actttcatta gaaggtctgc ctgaagttcc tattcattat gaattaaaag
     1441 gtgccgattg tttgtgtgaa gactgtggtc atactttaca tgatatcggt gcgacaaggc
     1501 ttagagaaga gccattgtat gttcctgccc atatagaaaa acaagtcatc taccaacatg
     1561 cctatcattg cagagaatgt gaagcattag gagaaacaaa aatcaaaaaa gcgcctgttc
     1621 ctaaaccgtt gatcaataat agtctaggtt cctcttcaat tgtgacacag agtatttggg
     1681 aaaaatttgt taaaaaggtg ccggcatatc gacaagaaaa agaatggcat gcacttggtt
     1741 tccctcttga tcgtaaaaaa atcactaatt ggcatatcaa ggtaagtgaa atggttttag
     1801 agccaatcta caattgcctt catcaagaat taagcaatca ggaggtctta cacgcggatg
     1861 agacaagcta tcgggtattg aatactcata aagaaaagac cttttactgg ttatatgctt
     1921 caggtaaaag cgaagaaaaa caagttgtgc tctacgagca cgcaaattcg cgttccgttg
     1981 atgagccaaa aagtttttta gaatcattta gcggtttctt gcatactgat ggttacgcgg
     2041 cctatggaaa gctgccggta aacaatgttt attgttgggc gcatgttcgc agaaagtttt
     2101 ttgaagcatt gggtaaacaa ccgtccccaa aaagtcatgc caaaatagga ttagacttct
     2161 gtgacaagat gtttcgaatc gaacggacct ttaataagct tagcgcagaa gaaaggtttg
     2221 tagcgaggaa ggaaacgttg cagcctgtga tgactgaatt ttttcattgg tgtgagagtt
     2281 ttgcttattt gccaaaatcg cagcttggaa aggcggttca atacgcagtc aatcataaag
     2341 acggacttca gattgtgttg cttgatggac gcttggaact ttcaaataac cgagcagagc
     2401 gtgccatcaa agagttagtg atcggtcgaa aaaactggct attttctaaa agcttaaaag
     2461 gcgctagatc aaatggaatt atattgagta ttatccagac agctgttgcg aatgggttaa
     2521 acatccgcaa atatttaaat catcttttta cagagattcc caatctgtct tcgatgacac
     2581 ctgaagcgct acgcgcttat ttgccatgga atcagcagat acaagaaatc tgtaaataat
     2641 ataagccaca aataacctga tttgagtgta tcaaatcggg ctatttgtgt ttaggtacca
     2701 ttctatgaca cgcttacgct caaaactact gaattattaa gttatgtcta atcaaataaa
     2761 aaaaccattc tcatatttgc tgaataatta gcatttagag aatggttttt tatctttaaa
     2821 atctactact caaaatctgc atgtcgagca aacatcgttt ctgagctttc acctgtttta
     2881 tccattaaag ataacactaa actatcaaag gtgagaaatg ctaattgttc aaaacttgaa
     2941 cccataggtt gtgaaaaagt atcttcttct cgttgatttt catctttggt aatacctggt
     3001 aatacaagaa cagaatctgc taattgacca atcgacgagt cttttttcat ggtaactaaa
     3061 gcaatgctta caccactttg atttgctttg gccgctaaat tttttaaact acctgtttca
     3121 cctgagccag aacagataat taataaatct ccaggttgcg aatgtggtga actaatttca
     3181 ccgacaaggc tgactgaaaa acctaaatgc attaagcgat tcgtaaatgc ttgcatggca
     3241 attccagagc gtcctgcacc agctagaaag atgtgtttag cttgaacaat tttctcacta
     3301 aattgttcta actctacaga attaatacgt tttgagttat tttgtaattc taagataatc
     3361 gcttctaaat agtttttatt ttggtcttta ttggtcatga tcttttactt cacttcctct
     3421 aatgattgat ttagtttatc aatttgttct tgcggggtat ttttcccatt gaagatgaaa
     3481 ccgcctgata caaaaatatc cgcccctgct ttagaacaaa ctttaatcgt ttgattatca
     3541 atcttgccat ctacttcaat atcatacgta taatcatttt gtttgcgtat tacatctaac
     3601 tgttcgattt taactactgt ctcttgaata aaatgcttat tctctgttcc tggattgatt
     3661 gtcattacta atacttgatc gactaaagat aatactggtt caataaaact gactggtgtt
     3721 gcaggattaa aagtaatccc tacttttgct cctgcttcct tgactaactg aatgaccttc
     3781 attgcgtgtt tggtagcttc aatgtggaag gtaacaatat ctgctcctgc ttctacaaat
     3841 cttgtgacaa ttttttctgg tgtatcaatc atcaaatgaa catcaaacac aacgtctgtt
     3901 agtggacgaa tatctttaat aacacttggt ccaaaagcca tatctggtac aaaacagcca
     3961 tccattacat caatatgcaa ataatcaaca ttcttttctt ttaagatttc aaataactct
     4021 ttaacttctg cgataggtgc atttaacaat gatggagcaa ttgtcattct ctattccccc
     4081 gtttattctc ccataatcat gagattgatt gttctagttc gctctcttag ttgatcccaa
     4141 tcgacaaaat agacacggcc catttctcct aataaaagtt taccttcttg aacaattaaa
     4201 gtcattgagt tggttccata aatagatgaa cgtaaatgtg catcggtatt tagcatgacc
     4261 catttttctg cagggtaatt gggatcagat aatcttgttc caaaatcaat gtgttttggc
     4321 cccggactgt tgtattgatt ttcactcgtc atttttggaa taatcgtttc cattacattg
     4381 ttgatgtcta catgtaaata atcatcacca aaaaagtttg tgtcatgcat cgtttcttca
     4441 aaaaagagtg agcaagttgt atgcgggctc gaaattacta acattccatt ctcaatacct
     4501 gctgtatcaa ctgtatctaa aagttctttt cgaatatcat gatacgaagg acgccctgaa
     4561 actgtttcaa tagtcaaagt gtcatgatga aaacgcatat taaacaccta atctttcttt
     4621 tacatctgca taaatggcat ctgtaccaat acctgttaat aagcccaagc cattcgttac
     4681 tttagcttta attacatcag ggacaattgt tgtacttact acagcatcat attcatcaat
     4741 tttatttagc tcatcagcaa ttttagattg ataaattttc acttgatctg ttaatccttc
     4801 ttcttccaac cattttgata attttgctac tgcaattgta gaagtagcat gacctgctcc
     4861 acaaataact aataattttt tcatattgat ctcttctttc ttattttaat tttatgatta
     4921 agctttcttc actttgttca tgtagataga agcacctact aaaacaactc ctaatccacc
     4981 tattccaatt acaccaaagt aagatgctaa taaaacaaac aaccctactg gccataatcc
     5041 acataacaac atagttacta atcctgctgt ccccacatcg aaacttgcta atgtaaacac
     5101 atcagtgata attggtgcca accatgttgc catatataat gttgtaacaa catacaacga
     5161 acatcctact aatgatctca caatatttcc tttaaaataa ggaatcatta aacagatggc
     5221 gaaagcaaag aatgctaaat ctccaaaagg taatacttta tttcctggaa gaattagtgc
     5281 tagcaataat gagattggta caagcaatag agaagttgac ataatagttg gatgacccac
     5341 agttaacgct gcatccattc caatgtttac ttggcgacca tttaactttt tattcgtaaa
     5401 ttctttcgcc gcttctgcaa ttggcattaa accttccata aacattgcta ccattttagg
     5461 cataatcttc atcactgccg cagtcataat tcctaagttt ccaattcctg ctacgtcata
     5521 gcctgccata atacccacta caataccgat aattaggcca ataacaactg tgtcacctaa
     5581 aacaccgaaa cgttttgtta atgtttctgc atctgcatct attttattaa atccaggaat
     5641 acgatcaaaa acccaattta ctggtttggc aaatagtact cctgataatc ccatcatatg
     5701 tgtgatagag attcctggat agccgaaaaa ttcgctaaca gttttttgtg tacgatctgc
     5761 taagaaccat tgtaaaatta ctgcgacaat cgcgccaatt aagccaaacc aaaagtttcc
     5821 cgtaacagat tgagtaatca tacctaggaa aattgcgaac caaaagttcc aaatatcaac
     5881 attcaatgtt tttgtccaac ccaaaaccac aaatatgaag ttaagaatta atgaaattgg
     5941 aatcattaaa attcccaatg atgtagagta agccaaaggt ccaccaacac ctacgccaat
     6001 atctaggata ttcaggccac caccgtattg ttcccccatt tgttgaattg ctgttcctaa
     6061 accatctgat aataaactta aaactaatcc taaaccaata gaaccgatac caacagtaac
     6121 gccggaaaca aatgctttac caggttttaa tccaaataat atcccgatta caaaaataat
     6181 cagtggtaaa aagacgtttg accctaatcc aataaaccat tcaaaggcat ttaatacaat
     6241 atccatctct ctattcctcc taataaactc ctgcagaatg tactaactca ataaatgctt
     6301 ctttcgtggt acatgcaagt aattcattta ctttttcttc attttgaaat aggctaacta
     6361 agttttgtag tgtatcaatt tgttcatgag gttgtgacat tgccaacata aagactaaac
     6421 gtacaggtat ttcatgagat ttatctgtca tatctgaaaa taccagtggt tcctttaatg
     6481 aggcaaaaca gatttgagag ctatttacat gtgcggaatc tgtatgtggg atagcaaacc
     6541 caatttgatt tacttctaat ccagttggaa attcttgttc acggttggtg ataccttcta
     6601 agaaactatc tttcacgcaa ccagcgtttt ttaattgttc agacattact tgaaacaatt
     6661 ctttttgatc tttcacttct aattgaaata aacataattc tttatctaga tacattttta
     6721 ttctcctttt tgttgaaaaa ttcttagtaa tttttcgcta tcatgaactt gtaacaagcg
     6781 atcgaccatt tctttttgcc caataaattg gtaaagttct tcatatactc ctctaatctc
     6841 agatacatct tcttcagcta aactaaccat aatgactaat ttgacttttc gatcgcccca
     6901 attaatcgca ttttttaatg ctacaataaa aatgttggat tcaatgacca ttgatggatt
     6961 cgcatgcggt aaagcaacac cactttctaa attagtattc cccattttct ctctagagaa
     7021 gatagaagca cgaaactctt cagtaactaa tgaattggct tctagttcgg aaatcatcaa
     7081 atccaaacat tcttctttgc tttccacctg tactcctgtt tttactaaag atgattgaac
     7141 aaatttagat atggtgggaa ctttaaccgc tttaggaatc agttcatttt gaataaatcg
     7201 cgaaggttct tctattaaat atttagtata agcttccata atattcatat aatctgtttt
     7261 acttaccaat ggagataccc ttactaaggg tataccacct gcatcaaagg aaagagagga
     7321 aataatcaaa tcaacttccc caatttcagc taaatgaaac ttatctaaag ttgatatttc
     7381 tacattatct tttgctggta aaaaccgtcg aactttattg taaattaatt gcgcagaaga
     7441 aataccgtat tggcaaataa tgatgatatt atttgctttg gactgatctt caatagcaat
     7501 ctgaaaatga atcaaaatta aggaaacttc atcgtccgtt aaagtcacat caaattcttc
     7561 ttcaaggatg gacatggcgt accatactat gctaaagagt tctgaatatt gagatttgat
     7621 attttctaat aacggatttt gaattttaat accttttttt aaacgaagga ccattgctgg
     7681 cacatgatac aataaggatt gatataatcg atcattttga gtcaaatcaa ttttttcaat
     7741 ctcactcatt ctttcaatca atttatgaac gctcatagcg tactcttcat tgctggtttt
     7801 taaattattt gtcacacgat gtgcaaatag ttgacgacat agatattctt tatctgcgtc
     7861 ataaaatgga agattcaatt ttgccttcaa ttcttcaatt aaaccatttg ccacaatgta
     7921 cgtttccata tagcgaatgc tattgaagag gaattcttct tcaacagtta aatgatgacc
     7981 tttcagtaaa cggttacatt ggataactaa agtagaaatc aatgaccgaa catagtaatc
     8041 agaaaccttt tcagtcagtt cactgtaatc attgaatagc aaatcatgaa tgacactaat
     8101 aacttttgaa tcaaacaaca tatttaaaac ttgaaaaaat gtttttgatt ccatgtcatt
     8161 cgcataatag aatacaagtt gcttgattgc tcgttgaatt gcttgttcat taccagtaaa
     8221 actaattcct tctgcagatg actgcaactt aacttctcca ctgccaataa ttttattgat
     8281 aattctcagg tcattgtata acgatgtttt actaaccata aaactttgag acaaatgagt
     8341 taaagaaaca ggcttatcac caaacaaaac ttctttaatg atct
//
LOCUS       DACDHJ010000105         8336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDHJ010000105 DACDHJ010000000
VERSION     DACDHJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.107"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..1410)
                     /locus_tag="IV223_001779"
     CDS             complement(118..1410)
                     /locus_tag="IV223_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8712063.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1407..2030)
                     /locus_tag="IV223_001780"
     CDS             complement(1407..2030)
                     /locus_tag="IV223_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712064.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2033..2869)
                     /locus_tag="IV223_001781"
     CDS             complement(2033..2869)
                     /locus_tag="IV223_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8712065.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2886..3773)
                     /locus_tag="IV223_001782"
     CDS             complement(2886..3773)
                     /locus_tag="IV223_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8712066.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3781..5016)
                     /locus_tag="IV223_001783"
                     /pseudo
     CDS             complement(3781..5016)
                     /locus_tag="IV223_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5699..6382)
                     /locus_tag="IV223_001784"
     CDS             complement(5699..6382)
                     /locus_tag="IV223_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8712067.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6400..7017)
                     /locus_tag="IV223_001785"
     CDS             complement(6400..7017)
                     /locus_tag="IV223_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8712068.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7027..7851)
                     /locus_tag="IV223_001786"
     CDS             complement(7027..7851)
                     /locus_tag="IV223_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8712069.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7844..>8336)
                     /locus_tag="IV223_001787"
     CDS             complement(7844..>8336)
                     /locus_tag="IV223_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712070.1"
                     /translation="LDEYLLISPEQWQKWKDGEKNLPSEAMQKVKNLFTDYEWMLTQK
                     ILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKAAKVELIPLKNNQQAGRYLDLKVS
                     IQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWVNENLEETYVHEANGEREEQRKDF
                     SSE"
BASE COUNT         2459 a         1845 c         1411 g         2621 t
ORIGIN      
        1 catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa aagacagcct
       61 atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa tctattctta
      121 ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa tagaatgatt
      181 tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg cccgattcca
      241 cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat ctcctccgcc
      301 atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca tcggatagac
      361 atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg cattgatgta
      421 agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag aaacattgaa
      481 tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag tttcatattt
      541 gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac tgcggtcacg
      601 aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct cattggctga
      661 caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc gactgtaaaa
      721 tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc caccgaatgc
      781 aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat gatgggtaga
      841 tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc tgaaataatc
      901 tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat caccaatcac
      961 atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat aattaaaagt
     1021 gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag ctttttcctc
     1081 atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg cacgaatgat
     1141 ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc gtacgtcatc
     1201 cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc catacttgtt
     1261 cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa aaccgactag
     1321 ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg ctctcccacc
     1381 aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc ttttaagcaa
     1441 aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa atcaggctgt
     1501 atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt gacgtttttt
     1561 gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt atcttctttg
     1621 cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag acacaataga
     1681 aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc aactgcaaaa
     1741 ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat tttccctttc
     1801 ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga aggatacatc
     1861 atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa agtgaacaaa
     1921 catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa aatccgacag
     1981 acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat attcagccct
     2041 tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag aaaatcagaa
     2101 aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc gtactagatc
     2161 cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc ttgtttaaat
     2221 aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg atcgtcgtga
     2281 tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac ttgctgcatc
     2341 cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat tgcatgatct
     2401 ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca tacgtcccaa
     2461 ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt gcaggaatca
     2521 tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg attctcgata
     2581 atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact gcagagatga
     2641 caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc catccttttg
     2701 cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc ataatctctt
     2761 gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg actagaccta
     2821 atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt tcttcctcct
     2881 ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt gacagctccc
     2941 ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt aaaagaaaaa
     3001 gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc accttgggta
     3061 atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac caagttatac
     3121 aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca tttgttcgct
     3181 ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc tgcagcaaag
     3241 atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat catgaccagt
     3301 ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa aatattcact
     3361 aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact gccaccgatc
     3421 aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt cctcgttaaa
     3481 agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa tacatagaga
     3541 aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat ataattatcc
     3601 aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata ataaagtgac
     3661 aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga agcaaatacg
     3721 taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt catgccttcc
     3781 ttctgctgtt acttcttcat aaatgacttt ttcaactaaa agattgtatt ggtcttgaat
     3841 ttccggattc aaagcactat caaagacatt cacttcgccg ttgtccatat cggaaatcat
     3901 ttctaaataa tcaaatgtcg cttgcaaagt tgcatcatct gcacgagcga gctgttctcg
     3961 aatattcgac atgattggta caccacgttc tccttttaaa atttcattgg cttcttcact
     4021 gttttgaaag aaatcaataa acttagcagc ttcttcttta tgggctgagc tttttgccat
     4081 actcagcatt tgcgaagaac gtaaagggat accagattct ccatctgctt ttctgcgagg
     4141 tggattaatc aactgaatct ccatatcttc aggagcccct gacaatattt ctgtcaactg
     4201 gttacttgat agaaacacca ttgccgcttc tctatcccta accgcctgaa aagacagatc
     4261 cgtcgtactt gcagctccac cacctggttc tggatgggcc cctttgtcta ccagattttt
     4321 acgcagctgg aaaaaatctg ttagatagtc tggattttca aatcccaagc tgttagatag
     4381 atcttcccga ttataaaaat tcaatccttt ctcaacttgt ggtatttgaa taatacagcc
     4441 agctataaaa tcttcgaatc ttgaaatagc atagattcct aattcttcag tgatttgata
     4501 gctgatttgt tcaaattcat cccatgtcca atttgaagct ggttcaggga tatttaattg
     4561 ttcaaatata tttttattgt aagcaatcgc aaacgaattc accccgttag acagtccaac
     4621 taattgcccg tcgtaagtcg tcgttgctaa ataggattca tctgcattag aaatatcaat
     4681 tatcccttgc tccacatatt catctaatgg ttcaatctga ttgatgtatt gaggaaattg
     4741 attgttcagt tggaaaatgt cccagatatc atctgatgct gctagtgtac ctaattttgt
     4801 gtaatatcct tcataatcat aaaattcata atcgatcgtc acatggggat ttttttcttc
     4861 ataaagctcg attactttta ctgttgcatc gtgtcggact tgcgaacccc accaagccat
     4921 gttcagcgtg atttttttgt catcttcttg cccacaagcc gtcaagaaga ataacataac
     4981 acttaaagct aagatgattc tcattctttt cttcattctc tgcacctcta cttccattgt
     5041 tatttttcaa tatactgtat ttttatacag aacatattta ttaaacgatc tttttcaagg
     5101 ggaataaaaa ccaagacttg ccttatcgtt ctgcctagga tgaacaactc aatactttca
     5161 agaaaaatta gatagtgctg attcttcttt catcgctaaa aacacttctt gcttcaatga
     5221 tgggaggata attacgcaaa gaaaaaagga tatcagacaa ctgcccaata tccttgacat
     5281 agctaaataa gcatgcttct tcgtgtgcgc tcttccctaa aaaacagaaa aataactagt
     5341 aattctgtat gaaattaggt acaatataca cgtcgatgca caagcattga ctatggcaac
     5401 ttgattggtc agttcccgct gacttttcaa gccccgacaa tttagctgct actaaattgt
     5461 cgggtgcctt ttttagtttt tattcgtttt tcattctata gaaagcgctt caataaatca
     5521 agacattttt ttgttagaat gctaataata acaaaaggtt ttaactcttc ttatgtacat
     5581 tacaggtaaa aaaacaaaaa aatttctctt ttttttgaat tctgaacaaa tagtactgga
     5641 gtacaaaaga cgaggaagag ccactatctg ctcttcctcg tcttttgatt ctcacctgtt
     5701 attcatgatt ctttttctca gtttcccgat atttttccat tttacgacta aaaagctctc
     5761 cgctacttgg tggcgtatat gtgatggcat ttgctcctgc ttgaatggtc tcagcaatac
     5821 tttcttccgt tggaccacct gtcgcaatga taggaagttc aggatatttt ttacgaaaat
     5881 ggcgtactgc ttctgcagtt tctttacctg cactgatatt gataatcgaa ataccagctg
     5941 ctaacttttc atctatttct gtatatttcg acgttaccgt actgataact ggaatatcaa
     6001 ctactcgaca aacttctttt actgtctcta aaggcgtagg accgttcaaa acgaccccta
     6061 acgaaccttg cgcttctgcg aacatgctca tgtaggaaga acgagcacct tgtgtcaaac
     6121 caccacctac accggagaaa actggaatgt cagctgcctc aataatgctt ttagtaatcg
     6181 ctggatgagg tgtaaaagga tacacagcaa ttactgcatt cgcatttgta ttacgtatga
     6241 tcgcaatatc cgtagtaaag atgatcgaac ggatcaattt tccaaatatc ttgatcccgc
     6301 ttgcttctaa aatgatttcc ggtactctta cgatatcttt tctcaattcc gtcatgattt
     6361 ctggcgtaaa ttgttctgcc atcagccact tctcctttgc tatctccggt catactgatt
     6421 caattgatac gtctcgatga cctggatgat tttttgcgca taaccggggt ctgtcgcata
     6481 acctgcatct tgcaatgctt gtgcggcttc ctgatagttg gtggccgtaa tcactttttc
     6541 atacagcgca ggattccaat ctactccttg gacgaacagt tgtgtatggt catccatgga
     6601 ctgttcccat gaatcataca ctttgaagtc tccttggata gttatccatt cttcgttaac
     6661 aaattccttt gtttccaggc ttactttctt ctgatcccca taagccttga tcccgaaaag
     6721 attattgtat ttgctagcca aggtactttt tccccaattt gattctaata tagcttgtcc
     6781 taaaatgata ctaggaagca ctccataacc atcttgtagt tcttgggcat gcggaacaag
     6841 ccgttcaata aattgctgat gggattgcgc ttcattttct tgctgattat cagtaaaagg
     6901 agaagacaga ttcttcaagg aaaaaacaaa ggctaagagt atcactgcta caccggcaac
     6961 cactgctggc caacggatat tcttgcgctt ttgctttttc cacttttttt tcgccatgat
     7021 caattcctac ttttcattta attcattcag atattcttct agcgtcaatt ggtgtttggt
     7081 gatatactca gcgttttctt taccgacata acgtaagtgc caaggttcat atgtgatctt
     7141 agttatatct tcgtgtcctt ttggataacg aacgataaat ccatactgcg cagcattatc
     7201 tgcgatccat ttagctccag gctgatctcc gtaacttgca tccaatacag tagaaggata
     7261 actgttgtac cagttttgat cgacgacatc gactgccaga cctgtgtggt gttcgctgta
     7321 tcctggctcc gtaatcgttt ctttagtaat ctttgtcgcc tcctcttctg atacaccttg
     7381 acgactcatc acatcttgga cattttgttc gaaaacttgt tgttgggatg atacagaccg
     7441 gtaagctgag accatcacta atggaaatcc agctgcttca gcagccttgg caaatgcttc
     7501 atattctgga gcaatgcgtt tgtcgatcaa gtatccgtta cttaatgatg ccaattgagt
     7561 agactcatca atttcttttt ctaatttgtg atctggacca accaaaacta gcttccaatc
     7621 gtctgtttta acatctggca attgtttgga cgtacgatct tttgtttctt tgtcctttac
     7681 cttgctgctg gttgtttgac tgttcttttc tttactagca gttgttttgg tgcttgttcg
     7741 ttcgactgct tgattgccgg cttgaacatg cgaatcatca tcgctaaaaa taacatagcc
     7801 cattgctgcg atcataatca caactgctcc aaatcctaaa aatttattca cttgaaaaat
     7861 cctttcgttg ttcttcacgt tccccattcg cctcatgaac gtaagtttct tccagatttt
     7921 cattcaccca atcgagaagt tcgatttttt ctccttcgat ctgtttttga acatctgctg
     7981 gcagacgaaa attcaaaata tcatcatagc cccattcatc atattgaata cttactttca
     8041 agtcaaggta tctaccagcc tgctgattat ttttcaaagg gatcagctcg acttttgctg
     8101 ctttgctgct aagccatttt tgcgcgatgc gtgttttgat ttgcttgtac tctgcaacgg
     8161 caacgtttcg tttttcggga aaaataatcg tttgacgcaa aatcttttgc gttagcatcc
     8221 attcataatc agtgaaaaga ttttttactt tctgcatcgc ttcagatggc agatttttct
     8281 cgccatcttt ccatttttgc cattgttctg gactgatcag caagtattca tctaaa
//
LOCUS       DACDHJ010000106         8030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDHJ010000106 DACDHJ010000000
VERSION     DACDHJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.108"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..1224
                     /locus_tag="IV223_001788"
     CDS             70..1224
                     /locus_tag="IV223_001788"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8712071.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1246..1998)
                     /locus_tag="IV223_001789"
     CDS             complement(1246..1998)
                     /locus_tag="IV223_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8712072.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2362..5028)
                     /locus_tag="IV223_001790"
     CDS             complement(2362..5028)
                     /locus_tag="IV223_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8712073.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5369..6820
                     /gene="cls"
                     /locus_tag="IV223_001791"
     CDS             5369..6820
                     /gene="cls"
                     /locus_tag="IV223_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8712074.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRPIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7149..7964)
                     /gene="thiD"
                     /locus_tag="IV223_001792"
     CDS             complement(7149..7964)
                     /gene="thiD"
                     /locus_tag="IV223_001792"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8712075.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2324 a         1675 c         1444 g         2587 t
ORIGIN      
        1 tatttctttt aatcttttta tgatacctta gtaaagtaag ttttttatca taagtaaaga
       61 ggtgttgaaa tgaaaatagg gattgatcgt ctttcctttt ttattcctaa tttatattta
      121 gatatgacag agttggcaga aagccgtggg gatgatcctg caaaatacca tatagggatt
      181 ggtcaagacc aaatggcagt caatcgtgca aatgaagaca tcattacact aggagcaaat
      241 gctgccagca aaatcgtaac agaaaaagac cgtgagctaa tcgacatggt catcgtcgga
      301 acagaatccg ggattgatca ttcaaaagcg agtgcggtaa ttatccacca tttactgaaa
      361 atccaatctt ttgctcgttc ttttgaagta aaagaggctt gctacggtgg caccgcagct
      421 ttgcacatgg cgaaagaata tgtcaaaaat catccagaac gaaaagtact agtcatagca
      481 agtgatattg ctcgttacgg cttggcaagc ggtggtgaag tgacgcaagg tgtcggtgct
      541 gttgcgatga tgatcactca aaacccgcgt attttatcga ttgaagacga cagcgtattt
      601 ctgacagaag atatctatga tttctggcgt ccagattata gcgaatttcc tgttgttgat
      661 ggtcctttat ctaattctac gtatatcgaa tcattccaaa aagtttggaa tcgacataaa
      721 gaattgtcgg gtcgaggact cgaagattat caggcgattg ctttccatat tccgtatact
      781 aagatgggaa aaaaggcatt gcaaagcgta ttagaccaaa cagacgaaga aaatcaggaa
      841 cgtcttatgg ctcgctatga agaaagcatc cgttacagcc gacgaatcgg taatctttac
      901 actggttcat tatacctggg gctaacttct ctactggaaa attcgaaatc actacagcca
      961 ggagatcgca tcggtctatt cagctacggt tctggtgcag taagtgaatt ttttacaggt
     1021 tatctggaag aaaactatca agaatatctc ttcgcacaat ctcatcaaga gatgctagat
     1081 tctcgaacac ggatcactgt tgacgaatat gaaacgattt tcagtgagac gcttcctgaa
     1141 cacggagaat gtgcagaata tacttcagat gtaccttttt cgattacaaa gatcgaaaat
     1201 gatattcgtt actacaaaat ataataaaat cataaaaaga acccttcacg agtcgtgaaa
     1261 ggttcttttt ttatctgcgg accaaaggag cagtctatag gtcagcttag tcgtactaag
     1321 gatttctcgt ttttcactga atagtttgcg aatgtacctg gtcacaacag gatcttttgc
     1381 gtacgcgtca tagtatagct tcatcaattc ttcagtaatt gattcctgtg taaaataagt
     1441 aaaatctttt ttcttcactt tgaatccttc atcaattaag tatgtttcaa attgatccat
     1501 tacaactggc gatgtattag gatcttcttc tatcattcct agttgctttt cgataggata
     1561 aaaaacatca tcgattgatt ccactactct gattatcacg caccatttgc ttgacttttt
     1621 catcaatttt tctaattgaa taagatgtac caatgctgga tttgctgctg taaaaaccat
     1681 ttcatatgtc tcatttgatt ggataaattc ttctaacgac tgtttgataa aggaaacatt
     1741 tggcaactca tttttttctg cttctttttt tgcattttct agcattcctg aagaaaaatc
     1801 gatcaaatca tagcgattaa tttcttttgc taaggaaaga aatctgccag atcctccagc
     1861 aacgtctgct gctgtttcag caggcaggac tttctccgtt ttcagataat tttttacatc
     1921 ttcagcaata gttacttgcg attcttgctg gatttcataa tattcttcag caaattcatc
     1981 ccattctttt tcattcattt gatttcctct aattttttgt tatatgtttt cacttttcgg
     2041 aataaatcat tctcctcttt tatctattct atctcttttg atgaataata aaatgattgg
     2101 ctttccgtct atcataaata aaaaaaggga ctgtgaccaa agtcgtgtca aagtccctct
     2161 ttccgaataa acagtgtcca atcagatgac cctcagtatt agctcaaaat gctgatttta
     2221 caacttcttc cttaaacggt gttctcctgc caactcttcg gtcacaagtc acaatattca
     2281 acaaacatga gaagctgttt gttgaaattg caccttgtga ctcagaatta aacggcaggc
     2341 tcacaacctc gttgattcgt tttatttatt tccttgtact cgtacaatga tcaatttagt
     2401 gatttccatc aaaacaattg tcacaagtgc gattcccata catgtaaaga cttctgtcaa
     2461 tccaaatgct gatgggatag agaagacttc tttcagtcct gggatcattg ttaagcagta
     2521 aagcgcgcta catacaacta cagctagtaa gacacttctg ttagagaaga accctgcttg
     2581 gatagctgtt tgtgtgtttg aacgtgctgg gaatgtttgc aatgtccgac accaaatcaa
     2641 tgttgaaaat gacatggcta caccgaattc aggtgaatgc tgcatgccaa gccattgagc
     2701 aaagataaca gcaatcccaa tcagcacacc acgatacgtt acagaaatca atgttttgtc
     2761 cgtaaagatt cctgaatttg gatctcttgg cgggcggttc atcgtagcag gttcagcttt
     2821 ttctgtacct aatgcaattg ctggcagtga atcattgact aaattgatga acaacagttg
     2881 taatgctgta aatggattgg accagtcagc aactaaggca aagatgatcg caatgatcgc
     2941 acctaaattt cctgcaaata gataagaaat agctttcttg atattatcaa acacattacg
     3001 tcctacttct actgcgctga caatagaagc aaagttatca tccgtcaata ccatcgctgc
     3061 tgcatctttt gccacatctg tacccgttcc catagcaatt ccgatgtctg cttgtttcaa
     3121 tgctggtgcg tcatttacac catcacctgt catagctgag atatgacctt tattttgcca
     3181 agcacgaacg atacgaattt tattctcagg tgaaacacgt gcataaacag tgactttttc
     3241 taaaacttcg tttagttgat cttctgtcaa ttcatctagt tccgtcccgg tcaaagccag
     3301 gtttccttct tcaaaaatcc cgatttgttt tgcgatagca acagctgttg ttttgtggtc
     3361 accagtaatc atgattgttt tgattcctgc acttcttgct tctgttaccg cctgagtcac
     3421 ttcagcacgt ggaggatcga tcatagcaag caaaccaaca aggatcaaat cgtcttcatc
     3481 ttcaaatgat aaatcaaaat tatcaagtgg acggtaagca aaagctagta cacgtaatgc
     3541 gcgtgttgaa aaagcttcat tttgctcttg gaattttttc atcaattcat ccgtcatcgg
     3601 aacgacttca ccatttagca atactttttt actgcgttgg aagacgatat ctggtccgcc
     3661 tttaacaaac aagtaatttt tgttatcaat tgtgtgtcca gtagacatta attttcgatc
     3721 tgagtcaaaa ggaagttctg cttgtcttgg gtagttgttt cggatttcct gataaggctg
     3781 gtttaatttt tcactaaagt cgataaacgc tacttccgta gggtcgccca atttagtacc
     3841 gtcttcactg atcgatgcat cattcgctaa aacactgatt tcgatcaagc gacgttcgtc
     3901 agctgtccat tttgaagggt catttgtgaa atccccagta tcgccattac ctaaaaagta
     3961 gtcaacaact gtcattttgt tttgtgtcaa tgtaccagtc ttatctgtac agatgatact
     4021 tgtcgaacca agtgtttcaa ctgctggaag cttccgaata atggcatgac gttttgccat
     4081 tttattcgtt cccaaagata acacgattgt tacgatagac tgtaatgctt ctggaatagc
     4141 agctacggct acggctacgg caaacatcac tgcgctgacg atatctgcag tcatatctcc
     4201 tgtaccttct ccaagataaa tacgaaacag ttggatacct aaaatcaata aagacaatgc
     4261 caaaatagcc aaacttagtt ttttactgaa tttatccaga cctcgttgta acggagtgac
     4321 ttgctcagtt gttgattcca aaaggccagc aacttgtccg atctccgtat tattcccagt
     4381 tgaagtcact aagaatttac cgcgtccgta agttacgatc gttccactga ataccatatt
     4441 tgtacgatct ccgattggta ccgtacttgt gatatccgtt acttcttttt ctgcagaagt
     4501 cgactctcct gtcagcatac cttcatctac ttttaatgat cccgcatcta gtaaacgtcc
     4561 atcagcaggt acataatcgc ctgcgtctaa caacacgata tctcctacca caagttcgtt
     4621 tgccggaatc gttttttcct taccatcacg aatcactcga gcatcgggcg ctgaaaggtt
     4681 tttcaacgct tctaaagaac cttctgcttt ttttgtttgg acaacactga ttatagaatt
     4741 caacatcaat actgcaaaaa taactaagaa ttcgatgtag tcacccatga ccatctgaat
     4801 cagtgcgaca atcagcaata cgataaccat tgcatctttg aaagtttcta agaacaattt
     4861 ccaagttggc ttcttcggct tttcttttag ctggttcaag ccatctcttt ctaatttttc
     4921 cttcgcttgc gtggtgctca atccatcggt tgagctctgc gtttgctgca tcaacgagtc
     4981 gatcgtttct ttatagaccg cttgttgtgc ttgttccttg ttttccaaat ttcatccccc
     5041 attttttgat cttggcccta gtcctgtctg aaaaaaaaga gacctaagac aaatacgcat
     5101 ttgtcttagg tctcaccatt tcatacaata ccagaaaacc atttggttaa cttactgttg
     5161 atattatcgc aacaaacgtt gccggttact cccctaagga gatattcaac tgtactaata
     5221 tcctacctta ttaacctaca aaaagcaact actttagccc gatttaccta cattttcatg
     5281 aaaggagtga aaagtatatt caccttggta ggcttctttc ttctatcatg ctatactaat
     5341 gttattgtat gtaaggagga acatttttgt ggtatctagt attataaccg ccctttatct
     5401 attaaatgca cttattgctt tggtggctat tttgatcaaa ccccgagatg tagcagccat
     5461 ttgggcatgg ctcttagtat tgtttgccct tcctggggtg ggctttgtat tatatttatt
     5521 tttcggacgt ggattaacgg ataagaaaaa attctatctc cgacaaagtg acttgaaaga
     5581 attagaaaac tttcagtctt ttagaggaga taccattgaa cattacgatc ctgacatggg
     5641 cgataaagac aaacagcaat ttgttgactt cttctcttca ttaaatcgta tgccgctgac
     5701 aagaatgaat tctgtcactc ttctcacaga cggacaagag aaattggatt cactgcttca
     5761 agatctaaaa aaagccaaac attcgatcca tatcgaatat tacgcatttg tgacagataa
     5821 tatcggccag caagtcttac atgttttaga agaaaaagcc gcagaaggcg tggaagttcg
     5881 aatattatat gatgcatttg gctctcatgg cacaaaagca aaagatttca atcgtccaat
     5941 caaaaatggt ggacatgtcc atacatttgt tacctcacaa agggcattac ttcgtttccg
     6001 attgaattac catgatcacc gaaaaatcgt tgtgatcgat ggaaagatta gttataccgg
     6061 tggtttcaat attgccaatc aatatgtaaa tacaacaaaa aaattcggct attggcgcga
     6121 tacgcatata cggattttcg gtgccgcttc ttctttgctc cagcttcgct tcttaacaga
     6181 ctggaacgtc tcggtacctg aagaaaaaaa ggtcggctat catttgaatt atttctttaa
     6241 aaaagcagat cgagatgaat ctaagcttgc tgatacatcc atccagcttg tttcaagcgg
     6301 accgaataac gaaagggaac aaatcaagct ttcatttatc aaattgatta cttctgctaa
     6361 aaaacgtgtt tggatacaga caccttacct tgttcctgat gaaagtgtca ttgctgcttt
     6421 aaaaatcgca actgcctctg gtgtagatgt gaaaatcatg attcccaaca aaccggatca
     6481 tccttttatt tatcgagcaa cacaatatta tgctcggcag ctgatcaagg aaaatgtaca
     6541 aatccttgtc tatgagaacg gcttcctcca tgcaaaaaca ttgataatgg atgatgaaat
     6601 ctgcatggta ggttcagcaa atcaagatat tcgtagctac cgattgaatt ttgaaacaag
     6661 tgctgtcatt tacgatcctg agtttttaga agaacttgct actcagttca aagaagatga
     6721 gacacattgt tcatccatga caactgaaac agtcaaggaa atgtctaact ggctattatt
     6781 caagcaacaa atttctcgat tattttctcc aatcctataa tttttttatt caagtagaga
     6841 aaaatatact aagattaaag ggactgtgac aaaaatcttg tcacagtccc ttcttccgaa
     6901 caaacggtgt tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     6961 agctgttttt tgaacttgtt ccttgtgact cggagctgga cggcaggctc acgacctctt
     7021 tcttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat
     7081 aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcaactt tttcacaact
     7141 tctttctttt actctaattc accagctgcc caatgattga tggggccata tttgtgccct
     7201 acatttattt cttcacgaat tgcacggttt acatagtctt ttgccaatcg gatcgcttgt
     7261 tcaactgttt ttcctttcgc taactcagct gcaatacatg cagatagcga atcgccagtt
     7321 ccgttgatcc ggtcggtagg ataaaatggt gcatttaacc aaaatgattt tcctgattcg
     7381 aacaatacgt agtcattgac tgtttcttgc tgcgcatttt tagcatgatg tcctttgatc
     7441 ataacgtttt ttgcacccat atcttgcagt tcttttgctg cttgcacaaa atcttcttca
     7501 gctgctaatg tccgtccagt cagcttttct gcttcataaa aattaggtgt cagtacttcg
     7561 gccaaaggga caagcttttc tttcaagctt tgcaaagcag attcttctag taaaagattt
     7621 ccatgtttcg ttacgattac gggatccacg actaatggtc caaaatcata tttttggtaa
     7681 ttttttacaa ctgtctcaat caactttgaa tctgctagca tacctgtttt agctgcacgt
     7741 atttgaaagt ccttcgcaat cagttcaaat tctttgtcga taaaatctgt tggcaatgtc
     7801 acacttgctc cgattccgta agaattacct gcaacacatg cggtcatgac agaaacaccg
     7861 taaacatttc ttgtaaagaa tgtatgcata tctgcttgca tccctgcgct tccatcgcta
     7921 tctgatcctg cgatcgtcat tgcttgtaca tactgatctt ccatcaaatc gattcctcca
     7981 tttttataaa aagatttttc ataatactgt tactgtttct atcatatgtt
//
LOCUS       DACDHJ010000107         7998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDHJ010000107 DACDHJ010000000
VERSION     DACDHJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.109"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..1990
                     /locus_tag="IV223_001793"
     CDS             2..1990
                     /locus_tag="IV223_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8712076.1"
                     /translation="MQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAA
                     FFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRF
                     WYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLI
                     LLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNL
                     FKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEIN
                     QTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGG
                     GTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYR
                     RKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQ
                     LSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKI
                     DKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNN
                     FGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQE
                     KNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2050..2766)
                     /locus_tag="IV223_001794"
     CDS             complement(2050..2766)
                     /locus_tag="IV223_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8712077.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2993..3535)
                     /locus_tag="IV223_001795"
     CDS             complement(2993..3535)
                     /locus_tag="IV223_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8712078.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3540..3767)
                     /locus_tag="IV223_001796"
     CDS             complement(3540..3767)
                     /locus_tag="IV223_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8712079.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3846..5726)
                     /locus_tag="IV223_001797"
     CDS             complement(3846..5726)
                     /locus_tag="IV223_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8712080.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5915..6529)
                     /locus_tag="IV223_001798"
     CDS             complement(5915..6529)
                     /locus_tag="IV223_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712081.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6671..7231
                     /locus_tag="IV223_001799"
     CDS             6671..7231
                     /locus_tag="IV223_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8712082.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7536..7850)
                     /locus_tag="IV223_001800"
                     /pseudo
     CDS             complement(7536..7850)
                     /locus_tag="IV223_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         2430 a         1596 c         1297 g         2675 t
ORIGIN      
        1 tatacaaaaa atccgtttca ttcatcgttt catcgaatac ttcgcttttg attcgattga
       61 acaattaact cagcgttgga aaaaaagaat tcattggctt tttgttcata aaagttattt
      121 tttagttgca gcattttttt actgctttac cttcgtgcag attttcgttt tagaaccaaa
      181 agaaggttgg aaagaaacta tccaggttgc cttaaaaata tttactcagc gacaagcacc
      241 catgatcctg acaatcatca ttattatggg atttttcttc ttactattgt tgatttccaa
      301 tcgtttttgg tacgccttga ctgctacctt gatcattaac ttactgctca ctatttcgac
      361 agtaatcaaa atggaaatgc gggaagagcc tgtctttcct tcagatttaa aaatgctgac
      421 cggattgtca gaactattat cgatggttag cccagtctta ttaatcgtgg gcggattgat
      481 ccttctccta ttattgatta ctagtattat tgtacaacgt agactacaaa aacagtattc
      541 cttgaagatt cattggaaac gtcgatttat cagtatcgct gtattattag gatgtttttc
      601 gggtgtcttt ttcattaatc acaaaaattc gccttctttc ttgctgttta atttatttaa
      661 agtaaacaag actttcttta accagcaagg cgccgtaaaa gataacggac cagtcattca
      721 attcctaaac aatatcgaca ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa
      781 aattaatgga ataatgaaaa agtacaacac aaaggcagat gaaatcaacc aaacaagaag
      841 tgactggtta aacaatcaga cgatcgttct caatttaagt gaaagcttca gtgatcctgc
      901 acgtgtccct aatctgacag tacctacaga ccctattcct tacatcaaaa gcattataac
      961 agaaggaaca ggaggattga tgttatccgt cggttatggt ggcggaacag ctaacatgga
     1021 atgggaagga ctgacaggct tgagtatcag taacctgtct gcttcgttag ttacacctta
     1081 tacccagcta gtggagcgtc agaaaataag tccaaatatt accgatctat tcgatgaaaa
     1141 aatagccatc catcctttta cagctgctct ttatcgcaga aaacaagtgt ttgataaatt
     1201 tggttttgac aggttctatt atttagacag tccggataaa ttaacttata cggataaaat
     1261 agaacaaagc ccacgcatat cggatgattc agcatacaaa gaaaccttga aagctttaca
     1321 aagcaatacg gaaacgtcac aattcatcca attatccact atgcaaaacc atatgcctta
     1381 tgatgattac tatagtgaat tgaactacac tgctgaagga actgctgtca ttcctaatag
     1441 aagaaaagaa ttacaaactt atatgcaagg ccttcactat accgatacag cagtaaaaaa
     1501 attcatccaa gaaatcgaca aaatcgataa accgattacc tttgtttttt atggtgacca
     1561 tttacccgca atctatagtg ggaatgattc gaaaaaatat ggattagaac agcaccagac
     1621 agattatttc atttataaca acaagtatag tcgtgaacaa gcagctacgt taaataaaga
     1681 agttgttgct cctaataact ttggtgctat ggctcttgag catgcgaata tcaaagtaac
     1741 tccttactat gcgttgttga cagaagtatc taatcatcta cctgcattaa caattgatcc
     1801 tacagaaagc ttgtctaatc gttttaatgg aaaacaggtc tttgttacag aggatagtca
     1861 aattatccaa gaaaaaaatt taactgagaa acaaaaagaa attctagaag attatcgtct
     1921 gatacaatat gatcttgttg caggtaacca gtacagtgca acatgggcga cacaaaaaat
     1981 aaaagactaa caaataaact gagcaagggg ttgtgataaa agacatgtca caaccccttg
     2041 tttatctcat taatttgctt gtccataaac gatttgatta aagccatttt tcaattcatt
     2101 attcgctgtc tcgttgaagt aaagataatt atcgggattc ccctgatttt gttgtgtcac
     2161 caatactttt ggctgatttt gctgaaaccc gaatagatag acgtgctttt tcagatatgg
     2221 ttctgtctct gcatcggaat agacggctgt gatttgatag tctgcttgac ctgtcccact
     2281 ttctgaccat tctgcagaaa caggcactcc accaatagcc atcttccact ccccgttcaa
     2341 aatagcttga gggactttaa gaccatataa gtccacactc atttgattcg tataactttt
     2401 gtattgttgt cccattgttt ggccccattt ggtcataaat gcctctaatt gtgcggattt
     2461 gtctgcattc cacagtgttt gttcaacttg ttcgctgctt tctgaagagg aagatgaagc
     2521 ctctacagaa gaacttgaag tagatgtttg agttgtttca gtcgttttgg aagactctga
     2581 cgaagtagag gtatctgctg tttctgtact tgctgcagtt gacttgctct ctgtgctagt
     2641 tgttgtagta gttgcaactg ttgctttcgc tgtttctttt gttgattctt ttttcgattc
     2701 attcccgttg gtacaaccag ctaacaaaaa agctagacca attgttacta aaatcctttt
     2761 tttcaattaa aaaactcctt tcgattactc ccacttcttt attctatcaa ataagcaatg
     2821 tgattttatt gatttttcgt attttctcat caatctttca aatcttctaa aaactgagaa
     2881 gcacccgctg atgttgtctg taatacatca gcgggtgctt cttttatttt aatcttcgtg
     2941 attttccaat ttacctcacg ctttatattt gtatttgaag aaacatagac ttttagtcat
     3001 catcgtcttc ctcatccaaa tcttgtcgga cagtttttcc ttggagtctt tgtacttggt
     3061 agatgatttc tttattgaaa cttaaaagtt caacaagcaa ttgttgcaag ccatattttt
     3121 cttctttttc agctaatttg attgcccgtg tgataaagac attttggaaa tcaaaatctt
     3181 ttgcaaagtt ttccaacatt gttgaggctg gtaaatattt gttttgacca tgttcgtaaa
     3241 tttttccata acgcatgact tcttctaaag tagacggaac gatttcattt tcaatcacta
     3301 actcatgaga gattttattg aaccatctcg cttcttgatc agcatattct gaaaaaaggg
     3361 tacttaattc ttgcgaagat gtacccaaaa catacaattg tgcttggatc aatttcattt
     3421 cttctaattt aaaatttgct aatacatgcc ccaccatggc gccagcagtt ggtttatgat
     3481 ggtcgatatc tgcttgtttt gtttcttgaa caaatttttc ttccactata ttcattgatt
     3541 tacgcctctt ttacttttgc tgattggact tcataaccta atttttccac aactgcagct
     3601 aattgttcag ggtggacttc atctggatca aagtttgctt tgattttgct cgcattaaac
     3661 aaaactttcg tattgcttac cccaggctgt tgagaaaccg catgttcgat tttttgcata
     3721 cagcttggac aagctagtgt atctaattgc ataataattt ttttcatcgt agttcttcct
     3781 tcctttttct tgatagtctt attctagatt acagcttcca taaaaaaatt gattcttatc
     3841 aagttttacg atttttcttc tttgctttcg tgtccaaaat acaataaacg catcgcgtta
     3901 aagataacga tcaagatact agcttcgtga acgaacatcc cactagccat gtaaataaac
     3961 ccggcaaata agccgttgag taaaaataag acgacaccaa tggcaatcac tacattttca
     4021 cgggtattta aaacagtttt cttagccaag cggtaggcat gaaccaacga ttcaaaatta
     4081 gattgcatca agacaacgtc actcgtttcg atagcaacat ccgttcctga tcccatagca
     4141 atcccgatat cagccgcagc caaagaggga gaatcattga ttccatctcc aataaaagca
     4201 acgcgcaatc cttgttcttg gtattttttc acaaattgaa ccttttcgtc tggtaataat
     4261 tcagcatgga cttcatcaat gcctaacata tcagctacat aatcagcagt catctgatta
     4321 tcacctgtca gcatcactaa ctgttttgct cctgcttgtt tcaacttagc taattgttca
     4381 gcgacttctg gtcgaatcac atctgacaca cctatgattt gagtgatttt tttgtcgatt
     4441 gctacaataa tgaccgagct gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt
     4501 ggtgtgagag aaagtttgtg agcggcaatc aatttggtgt tgcccgcaca aactgtatga
     4561 ccatcaatct cagctgtgat tccttgtccc ttgattgtct gattatcttt gactgctaat
     4621 tgttgaaaat caatgtcttt ctcctgagca taatggatca cggcgcgccc cagaggatga
     4681 tcggataacg tttctaatcg agccactaaa gctaagctgt ccccttcatc ctctgtatag
     4741 ttcttgatag tggatacagc agtagttcct ttggtcaatg taccagtttt atcaaagaca
     4801 aaagtatcga ttttggaaaa agtatccatt gcttcaccgc ctttgactaa gatgccccgt
     4861 ttcgctccat taccgattcc tgctacattc gaaacaggcg caccaatgac taaagcgcca
     4921 ggacaaccaa gaaccaaaac agtgattgct aaacgaaaat cacgactaat cagaccaaca
     4981 accaacgcaa taagtaaaac ggttggagta taatactgag aaaatcgatc aatgaatttt
     5041 tccgcatggg atttggtatc ttgtgcttct tctactaatt cgataatttt ggcaaaggtc
     5101 gtatcatctc cgaccttagt tgcttcaatc gtcacgtagc catctgctaa aatcgttcca
     5161 gagaagactt catctccact gattttgacg acttgtcgtg attcaccagt cactgcggct
     5221 tcatccgcat accctttccc gtcaatgact cgtccgtcca caggaatagc tccaccagtt
     5281 tttactaaga cgatatcgcc ttcttccacg tcatcgattt ccacttcttc agtcgttcca
     5341 tcttcttgga caacactagc agtagatggg gccatctcgg tcaaagcctt gattgactcc
     5401 cgcgttttgg tcaatgtttt ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg
     5461 atagctgact ccttgtattc tccgatcata aaggcaccaa gcacagcaat agtaacgagt
     5521 aaatcaattg agataacttt gactttcact gcctgatacg catggatcat aataggtatg
     5581 accgcaataa tagaagctat ggctagagca atgttataga acaagacaaa accagtaaat
     5641 tttccgataa aaccaaaaat aatcaacaca gctgataagg cggtgatatg atttcgatgc
     5701 tcttggatcc acttttgtaa tttcatcttt ctccctcttt tcctttcatt cttacgagac
     5761 taagtataga tacttatcca tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc
     5821 ggttttcttt tggtagaata cactacaaaa aatagttagc aagaagactc actaaaagtg
     5881 atcttgccaa ctattgtcgt ttctatcgaa attttcattg aatgattttc ctgtacagca
     5941 tacatcggag atctatttct ttatctaaga tttttatctt gctaattttt tcctttagtc
     6001 tttcaaatcc acgtcgttca tagaattgat acgtacagcc actatctgta aaaaggaata
     6061 tttcttttcc tttctctctt ttttcaaatt ctgttagcaa cttggtaccg atgccttttg
     6121 ttttcaaatc aggatttgca gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta
     6181 aatatgctgc aagcatttgt tcatttgctg tctcgtaagc tcctttactt tctttagcaa
     6241 atatattttg taaaaaatcg aatatcttaa catatatttg ctgtccaaaa gaacgatgtt
     6301 tctttttttc tccttttatc tccgccagca aaacacctgc caactcgtcc tctgcataca
     6361 aagcaagaat ttgagttgct cgattcaact ccatatacaa aaaatatctt ccgtaaagat
     6421 tcagcagaag ttcattttcc atatatctct caaagttcat tccttcaaca gcaaaataga
     6481 tagctttttt gtaatctttc ttttctaagg gtttaaaagt tagtttcatc atttcgcctc
     6541 ctcaacttca atgattgtgt tatacttaca ttataataga cacagtgtct actatctata
     6601 atcaatcatc tataagtttg tcgattaagc gacagctttg ctttgatatg tcggataagg
     6661 agataggtaa atgaaaaaga cagatagacg cgtcaaaaaa acagaaaaag ctttggctga
     6721 aacactatcc acattattag taaataaaaa gattcaagca atcactatac gcgaactaac
     6781 agaaacggca gatgtacatc gttcgacttt ttatacacat tataaggata tctatgatct
     6841 atatgatcaa ttagaaagca acttttttcg ggatctaaat gaaattctct cttatgatcc
     6901 agcccatagc tatgaggaac tatacactcg cttgattgac tatgtttacg caaatacttt
     6961 tttatatcgc ttatttatcc tcaaccatgg tgatatttcg tttcagaaga aagttaaaca
     7021 agttattgaa gaaaactatc tgaacatatg gctatatgaa gacaagaaaa cgattatcac
     7081 agaagaaatg agattcttta ctacgtacca cgtccaaggt tgtctatccg ttctcaatcg
     7141 gtgggtagaa agcaattaca agtactccaa aaaagaatta ttagaactct tacagaaact
     7201 gaatgatcac atagagaaaa tcatgccttg aataaaaaca tactaagctt agttaagaag
     7261 aaccgtaacc ttctcgtata acaactgaat cttatttagc caaattgttt actgaagatt
     7321 ttttgagtaa aatttctata attcctactt attgaaaagt agtttgtagt gctgcttctc
     7381 cctctctaag tgatatatct tgttgtacca atcaaattca cctcatcatt aataaattac
     7441 ttttaggcta atttattaca acttataggt tgaagctgta accaatttat agtcatatta
     7501 tatttttatc atccagttta ttgaattctt ctcgtttacc tcaattcatt ttcaagggga
     7561 atcagctcat catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat
     7621 ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct
     7681 ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc atgtaatttt
     7741 ttctcagaaa caaaccactc attggaaaag acatgaatca aatcatcact tacttgtgta
     7801 aaatagcgac gtttaatgac aaagatatct tcattgctat ctagtgtacc ttcatttctt
     7861 tgtgcttaaa ttcaaagata attttctcaa taaagatttt tgattccagt aagattcctt
     7921 tactcctttt acaacatagc gttgtccttt gttttgaaac atcttcttcc cctctttgcc
     7981 attaaaaata gcccaatt
//
LOCUS       DACDHJ010000108         7992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDHJ010000108 DACDHJ010000000
VERSION     DACDHJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.110"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            384..572
                     /locus_tag="IV223_001801"
     CDS             384..572
                     /locus_tag="IV223_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8712083.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="IV223_001802"
     CDS             650..1663
                     /locus_tag="IV223_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8712084.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="IV223_001803"
     CDS             complement(1730..2764)
                     /locus_tag="IV223_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8712085.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2880..5105
                     /locus_tag="IV223_001804"
     CDS             2880..5105
                     /locus_tag="IV223_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8712086.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5123..7006
                     /locus_tag="IV223_001805"
     CDS             5123..7006
                     /locus_tag="IV223_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8712087.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6987..7961
                     /locus_tag="IV223_001806"
     CDS             6987..7961
                     /locus_tag="IV223_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8712088.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2578 a         1459 c         1695 g         2260 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attgcggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaaataa aaaattttta ttgacaaaat tgtttagtaa
     2821 acagtataca ggaattataa aaaaaagtaa acggtttccg ttttgttggg agtgaaatga
     2881 tggatattac gcatagaaaa acagataaaa atttcatctt atttgatgaa aagagccgag
     2941 tcttccattt acgaaataca tttctttctt atctgataaa gatagaagaa agcaatgtat
     3001 tggcgcatat ttattttggc aaaccagtca agcagtacaa agacaataaa aattatcccc
     3061 gaagagatcg tggattttca ggaaatgttc ccttaaatcc tgaccggtct ctgtcgaaag
     3121 atacactacc gcaagaatac tcttctcatg gcagcatgga ctttcgaacg cccgcatcga
     3181 tcattcagcg caagaatgga tcagacttac tggatctgag atatgacagc cattatatta
     3241 cagatgggaa gccagagatc gaaggcttgc ctcaaactta tgtgatggac aagtcagagg
     3301 cacaaacgtt ggtgatttca cttaaggata gagaaacagc gatttatttc gatttgttct
     3361 ataccatatt tactgataga gcagtgatca cacgatctgt aaagataaga aatgaaaccg
     3421 gagaaacaat caaactagaa aaagcggcta gtttccagct agactttgcc catactaggc
     3481 gatttgatga agtcattgct ttgccaggag cacatgtcaa tgaaagacag atttcacggc
     3541 aatctgtttt atctggaaca aaagtatttg aaagccgtcg aggaacgagc agtcaccata
     3601 tgaataactt tattgcttta gttcatcatc atacgacaga aaatactgga gaagcgatcg
     3661 gacttcaatt tgtttattcc ggcaaccatt ctttcgaact tgaaaaagat cagatcaatc
     3721 aattgagagt cgtcggtggg atcaacagcc atcgtttttc gtgggaattg aatgcaggcc
     3781 agtcttttca aacacctgaa atgatccttt cttactcaag tcagggattg aacaaaatga
     3841 gtcagatcca ccatgagttg ctacgtgaaa gaatcgcaag aggtcgtcat caatttgctg
     3901 aacggccgat tctggtcaat aactgggaag ccacttactt tgacttcaat agtgaaaaaa
     3961 tcaaggcgat cattgatgaa gcaaaggagc taggcatcga aatgttcgta ttagatgatg
     4021 gctggtttgg aaaaagagat gcggacaatt cttcattagg agactggttc gagtatgaag
     4081 gcaagttgac aaatggatta cgtgaaatag cagattatgc tcatagcaaa ggattgaaat
     4141 ttggtttatg gtttgaacct gaaatgatct cggttgattc tgaactgtat cgaacacatc
     4201 ctgattttct tatgcaggag ccaggtagaa tgccttctgc ttccagatca caacatgtac
     4261 tggattttac aaggttagat gttcggcaga cgattgagaa acaaatgaga aagattttag
     4321 atacgattcc tcttgactac atcaaatggg atatgaaccg ttcgctttca gatgtctatt
     4381 cgattaccct tgacccacag agacaaggag aagtagcgca tcgttatatg ctcgggcttt
     4441 atgaactact tgaacatttg acaactgatt atccggaaat cttatgggaa ggctgttctg
     4501 gtggaggtgg acggtttgat gccgggttta tttattatat gccgcagtca tggacaagtg
     4561 ataacacgga tgcagtggag cgtatgaaaa tccaatatgg aacaagcctt gcttatccga
     4621 tttcgtccat cacagctcat gtatcagctg taccaaatca tcagaccgac agatctactt
     4681 cgttgaaaac aagaggagaa acagctatga gcgctgtttt tggttatgaa ctggatttga
     4741 caaagctgtc tcctgaagaa aaaaagcaag taaaagacca aattatatcc tatcaaacga
     4801 ttcgtccggt gatccagtat ggccattatt atcgtttggc gagtcctttt gaagaaaata
     4861 ttgccgcatg gatgtttgtg tctcctaaac aagatgaagc tattgtgttt ttagggagga
     4921 tactagcttc agctcaacca gcgttccatg aagtatatct gatggggtta gatgatgagg
     4981 cactttatca ggaacagacc tcgaagcgga tattttcggg ggccgaattg atgacagttg
     5041 gactttactt ccccgatttt caaggtgatt tccaaacaga actgcttcat ttcaaaaagt
     5101 tatgagagag aaggaaaaaa gtatgaaagc aaatataaaa tggttagatg atccacaagt
     5161 ttttagagtg aaccaatcaa atgcacatag cgatcatgtt tgttatgcaa caagggaaga
     5221 agtagaaacc acatcctccc ttaaacagtc tttaaatgga tcatggcgtt tttgttattc
     5281 gccaaatcct tcggtacgac caaaaacatt ttataaaaaa gattttgatg cctgttcttt
     5341 tgatgaaatc actgttccta gtcatattga attagcaaat tatgacaaga tccattacat
     5401 caatacgatg tatccgtggg agggacatgt atttcgccgt ccccctttca cgacaagccc
     5461 tgagaaagag gcggaaggga tcttttcagc tgctaaagac aatccggttg gttcgtattt
     5521 gacgactttt cgattagaag aaggtctaaa gggaaaacaa atctcgattc attttgctgg
     5581 tgtagaacaa gcaatgttta tttggttgaa tggtcatttt atcgggtatg ccgaagattc
     5641 gttcactccg tctgaatttg atttaacacc ttatttggaa gaagaaaata ttctagctgt
     5701 ggaggtttac aaaagaagta cagctgcatt cttggaagat caggactttt tccgattttt
     5761 tgggatcttc agagatgtct tcttagttgc tcgaggacaa aaccatttag aagatctttg
     5821 gatcaaacct cgcttagatg acaaatgtca aaatggcaaa cttgaagtag atttgtcttt
     5881 tgagcaagta gccaaaaaca gtcagctgaa tgtagcgata ctagatcctg atgggaagga
     5941 aatctatcaa cagcaatatc cgttaaaaga gcaaatgacg attcgcatcg atggatttga
     6001 aaatgtggca ctgtgggggc attttcagcc caatttatat aagcttgaat tggagacgat
     6061 agacgaagaa aaaagagtaa cggagtatat tgactattcc ttcggttttc gtaaaatcga
     6121 aatcaaagat aaaatcatct acttcaatgg aaaacggatg attatcaacg gggtaaatcg
     6181 acatgagtgg catccgaaaa aaggacgcgc catcactata gaagatatgg aaaaagattt
     6241 ggctatcata aaaaatgcaa agatcaacgg cgtacggaca tctcattacc ccaaccagat
     6301 cacttggtat tatttatgcg accaagcagg aatccatgtg atggcggaaa cgaacctcga
     6361 atcccatggc tcatggcaaa aaatgggcgc atttgagcct tcttacaacg taccaggtgc
     6421 agtacctcag tggcaggcgg ctgttcttga tcgagcaaaa acgaatttcg aaacattaaa
     6481 aaaccatcca agtattcttt tttggtctct tggaaatgaa tcttatgcgg aagaaaacat
     6541 tcgacaaatg aacgattact tcaaaaaagt agaccccgat cgacttgtcc attatgaagg
     6601 gaattttcca aatccagcat tcgaagattc gatttcagat gtaaaaagct gcatgtatgc
     6661 gccaccagaa gagatcattg actaccttga aaataaagcg gaaaaaccgt tcatcttatg
     6721 tgaatatatg catgacatgg ggaattcgtt aggtggaatg gattcttaca tgacgttgct
     6781 tgatcgttat gaaatgtacc aaggaggcta tatatgggat tttaaagatc aggcgatcca
     6841 agtgactgat ccaatcacag gcagagaagt gatgcgctat ggcggtgact ttgacgataa
     6901 accaagtgac tatgaattct cagggaatgg gatcgttttt gcagatggac aagaaaaacc
     6961 cgccatgcag gaggtaagat attattatga aaaatacagt aaataaaagt catatggata
     7021 cggaaaaagt tgcaatcgtc ttcggcgact gtacattagg tgtcaaatcg gggaatacgc
     7081 attatatttt ttcttataca agaggcggac tggaatcgct caataaaaac ggaaaagaat
     7141 ggctataccg ggaaacactt ccagtttttt ggcgagcaac gaccgataat gacagaggta
     7201 gcggatttgg atttaaatcc ggtatgtggt taggagccgg attgtatcca aaagtcaaag
     7261 aaatagaagt actggtggac aataaaaaga tcgatttgcc gattgctcca gtaaataatc
     7321 aatattcaag taatgaatac gctgaaacgg ttgaaatcat ttttacactg gaaatcaaca
     7381 cagtaccaaa aacccaagtg acaatttcct accaaatcgc tggagagggt aaaatgcttg
     7441 tcaatatgca ttattatgga caaaaaggat taccggaatt acccgcattg ggtttaaggt
     7501 tcattatgcc aacgaaagca aaattttttg agtatgaagg cctatcaggg gaaacatatc
     7561 cggatcggat ggctggtggt gtcgaaggta tttataaagt ggcaggactc cccgtttccc
     7621 cataccttgt tccccaagaa tgcggcatgc atatggaaac gaaatgggta gaactaacaa
     7681 gaaatcaaac gttggataat acggataaag atcaagaaga tttttcgttg cgatttgaac
     7741 agcaggatac gccatttgct ttttccgctt taccattcaa agcagaagaa ctggaacagg
     7801 caacacatat agaagaacta ccgccagaaa gacgaacggt tcttacgatt tttgcaaaag
     7861 taagaggagt cgggggtatc aatagctggg gagcggatgt tgagtcacct taccgaatca
     7921 gcagcgaaga ggaccatgaa atcggcttta ttattcagta gcaatcttta gattattatt
     7981 tgaaaaacgg ag
//
LOCUS       DACDHJ010000109         7952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDHJ010000109 DACDHJ010000000
VERSION     DACDHJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.111"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..768)
                     /locus_tag="IV223_001807"
     CDS             complement(<1..768)
                     /locus_tag="IV223_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8712089.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFY"
     gene            complement(992..2932)
                     /gene="thrS"
                     /locus_tag="IV223_001808"
     CDS             complement(992..2932)
                     /gene="thrS"
                     /locus_tag="IV223_001808"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8712090.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3269..3448)
                     /locus_tag="IV223_001809"
     CDS             complement(3269..3448)
                     /locus_tag="IV223_001809"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8712091.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3495..5318)
                     /gene="cadA"
                     /locus_tag="IV223_001810"
     CDS             complement(3495..5318)
                     /gene="cadA"
                     /locus_tag="IV223_001810"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8712092.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5596..7875
                     /locus_tag="IV223_001811"
     CDS             5596..7875
                     /locus_tag="IV223_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8712093.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2339 a         1694 c         1444 g         2475 t
ORIGIN      
        1 atagaaataa aaaatgatat cgatcgtagc aaattgggcc aaaagtgaat tagttgccgc
       61 acttcgattc gtgatttctt ttggtctaga tgtttgtacg agtacatcgg ctatcttgct
      121 aagcgggtta tttccaaatt gcgtcaacgc aatgatttcg atattcatgc ttttcgccag
      181 ctcggctaat gcgactacat ctgcacttct acctgagttt gaaacaagcc aaagcacttt
      241 ctttttctca ttgctgacaa gttgcgggag caagacgtgg atatcttttt caaaaatgat
      301 tggcttaccg acacgagacc atttttgcag aatatcttct actactaaag aagaggcgcc
      361 aaccccatat aaaaaaacag tctctgccga ttccaaacac tctacgactt tttggatcgt
      421 ttcttcttct agaatatcta ctgtttcttt tatcgctact tgtgcattgt taccaagttt
      481 gtttttgaga gagtaaaagg actcatttgc ttctatatca ctaaaacctt catcgattcc
      541 agtctgtagt tctgttgaaa tcgtgatttt gaagtccgtc agactttgaa aaccgatttt
      601 tttggaaaaa cgtacaactg tcggcgcact caacccggaa gcttctgcta attcattagc
      661 tgtcattgta gggaccttct caatattacc gataatgtag ttggccaact ttttttctgc
      721 tgaagaataa tcttccatca tactttcaat acgactaatt acgctcataa gttcctccac
      781 ccttttaatg atagtctatg taagtatacc atatcagacc cttattcacg gtcgtatact
      841 ggataaaaaa caaaaaaaca atccgcccaa cagaatatct tatcccatca atcccacaga
      901 ttctattgag catctgtgtc tttgaggaat acctgttatc tattgttcgg attgctttct
      961 atctactact tttttacagg aaatttacct attagttttc tctgctgtaa tttttgactt
     1021 ctgcttccat agcatcaata aagatattca aatcttctac agctgtttct ttacttccat
     1081 aacgacgtac gttcactgtt gcatcttcca attccttgtc tccaacaact aattggtaag
     1141 ggatcttttg tgtttgagag gcacgtatct tatagcccat tttttcatta cgatcatcaa
     1201 cttctacacg caaccccttc atttgcaaac gttctttgat ttcataagca taatcgccat
     1261 gtgcatcaac agaaacaggg atgattgttg cttgtatagg tgccaaccat gttggaaaag
     1321 cgcctttgta tacttctgtt aaataagcga caaaacgttc cattgtagaa acaattccgc
     1381 ggtggataac tactggacga tgcgtatttt ccccgtcttc tccaacatat gtcaaatcaa
     1441 agcgctctgg caataagaag tctaattgta tagttgataa tgtttcttct gtaccaagag
     1501 ctgttttgac ttgaacatct agttttggtc cgtaaaaggc tgcctcgcct tctgcttcaa
     1561 agtaatctag ttctaattca tccatagcgg ctttcagcat tgcttgggct ttttcccaca
     1621 ttgcgtcatc atcaaaatat ttatctgtat tgtttggatc gcgatagctc aagcggaaac
     1681 gataatctgt aatattgaag tcagcatata ctgccaccat caattcaagc gtacgtttaa
     1741 attcatcttt gatttgatca ggacgaacaa atgtatgacc atcattcaac gtcatttctc
     1801 gtacacgttg taaacctgac aaagcacctg atttttcata gcgatgcatc attcctaatt
     1861 cagcgatacg aattggtaat tcacgataag aatggatcgt gtttttgtag accatcatgt
     1921 gatgagggca gttcattggg cgaagaacaa gcatttcgcc atctcccata tccattggcg
     1981 ggaacatgtc ttcttgataa tgatcccagt gtccagatgt tttgtacaac tcaacatctc
     2041 ccattattgg tgtatataca tgttggtaac ctagactaac ttctttgtct acgatataac
     2101 gttcgatcgt gcgtcggatt gttgcccctt ttggaagcca gaatggcaaa ccagaaccta
     2161 cttctgggga aaccatgaac aagtcaagtt cttttcctag tttacgatgg tctctttctt
     2221 ttgcttcttc acggagacgg atatattctt tcaaagcttt cttgtcaaaa aaggctgtcc
     2281 cgtatacacg ttgcatcatt tgattgtttg agtttccacg ccaataagca cctgccactg
     2341 ataataattt aaatacttgt atacggccag tggatggaac atgtggtcca cgacacaaat
     2401 cgatgaattc gccttgttga taagcagtaa tcacttcttc ttctggcaat tcactaatca
     2461 gttctacttt gtacggatca cttgcaaaaa tttctaatgc ttcctgtttt gaaagaacac
     2521 ggcgttcgat cggcagattt tctttgacga ttttcatcat ttctgcttcg attgccggca
     2581 aatcttctgc agttacttgg ttttgtccat tatctgtatc gtagtaaaac cctgaatcga
     2641 tagctggtcc aacgccaaag tggatatttg gatagaggcg acgcatggct tgggccatca
     2701 aatgagctgc tgaatgacgg actaagccaa gtgcatcttc atggtctggt gtgatgatct
     2761 caatacttgc atcttcctcg attggacgag tcaaatcaac gagttttccg ttcactttac
     2821 cagctaaagc tttttttgct aagctttttg aaatgctttc agcaattgcc aatgttgttg
     2881 ttccagactc aaattctttg actgcaccat ctggaaaagt aatttttatc ataatagttt
     2941 cctccttgaa atgttttgat tgatcagatc atcaagctga aaaacaaaaa aatcctagca
     3001 tctcttgaaa agagatacta ggacgaaaag ttcgtggttc cacctaatgt tcaaacgcaa
     3061 atgcgttcct caagcgttgt aacggtccga ccgcccttgc ttcaacaagg gtttcgaaag
     3121 tggtcatcct ttggttttct cctggaaaag tttcagccta gcttttcctc tcttatcgaa
     3181 gactctcaaa ttcagttggc tttctcatca atcaattatg tgtttattta accactaata
     3241 aatagaaaaa tcaagtggga aactaaactt attttttcca ttccccattt acaccgtatg
     3301 ttcctttttt catctcattg atgatgacat ggatgtgttc ttttggtgcc tgcgtgtttt
     3361 tatgaacagc ttccgtgata tctttcatca tattttctaa ttgttccgga ctgcgacctt
     3421 ctactaattc gacatgtacg aatggcataa tgctctctcc tctttctgat cgttcttttt
     3481 tcaactcgat tctatcatac tatgtttgtc ttgggtactg aatcaggaaa aatggctgga
     3541 tctttttttg ctctcaaggc actcaaaata gagaccgtat ccacgacttc ttgcaaaaca
     3601 gctccgaata atgcggggat tagtcccgta cttgcaatta acatcaaaaa aatacaaata
     3661 aagatgccaa tcagtacaga ctgtttcgct attctcatcg tatctcttga aatcactact
     3721 gcttttgcta cacgcaagag atcatctttc aagatgaccg catctgcact ttcactagca
     3781 gctgtagaac catgagcccc cattgcaata ccgatatctg cagcagcaag tgctggtgcg
     3841 tcattcacgc cgtctcctac catcgcaaca ggtctttgat ctttagggat actttctaaa
     3901 atggtcagtt tatcttgagg caagctacgg gcatgtacag aatctaattg gactttttct
     3961 gcaatcgttt gagcaatggc agatccatcc ccggtgatca tcatcaaatt tttcacatca
     4021 agttttctta gctccttgat tgtctttgct gcttctgggc gaatctgatc cgaaaatagg
     4081 atcgttcctg caaaagatcg atcaattgtt acatagattg ctgtattgga cgtttctatt
     4141 tcttcagctg aaacaaaaga ggcacgtcca atctttactt catgattatt gactactgct
     4201 tggattcctt gaccggtcac ttctttcagt tgactaacag gatacagttg gctgtttttc
     4261 acgttgttca ctaacgaacg agctaagata tggctagatt cctgttctgc actagctgca
     4321 tacagaagta gctcttcttt ttttattcca ttgactggct gaatcgcctc tacttccaat
     4381 atccctttgg taagcgttcc tgttttatca aaagcaatcg tttttgtacg tgcgagcttt
     4441 tcaatcgttg ttccagtttt tacaacgatc ccgtttttac ttgaacggct cattcctgca
     4501 actaaagcaa tgggcgcagc tagaatcagt ggacaaggtg atgccactac cagtacctca
     4561 gcaaatcgta aagggtcttt tgagaaccac caagaaatac caccgatcaa ataagcaatc
     4621 aacgtgaaag ggactgcata acgatcagct aaacggacaa atcgggcagg cttttctttt
     4681 gattcttcga ctagtttaat aatcatttga tactggctgt ctgcggcacg tttttcagcg
     4741 atcatttgga tcgatccgtc tccatttaaa gaccctgaca taatctgatc tccaggtttc
     4801 ttggtttccg ggcgggactc acctgtcaaa gaagattcat cgaccgaaga ttctcctttg
     4861 gtcactcgac catctgcggg tacaagttcg ccaggcttga ctagtaactg gtcatcaata
     4921 tttatttcat cgattactat atcctgcaat tttccttctt tgaatcgatg ggcaattctt
     4981 ggggtgttat ctaacaaaga ctgcagttct ttacccgctc gtttattcgc gtaatcctct
     5041 agactatctc cgcccgtaag catgatcaat atcatcaaac tagcccagta ttctttgacg
     5101 gctaatgttg caaggattgc ggtaatcgct aatacatcta cgccatatct tcctgaacgt
     5161 aacgtcttga tcatttccaa aaacatcaag aaggcgagaa tccctccagt aaatgcgaca
     5221 atcgcaaatg caagggccgg ctggtgaaaa acaaattcac ttaataaagc tatcccacca
     5281 ataagcaaag taagtaaaaa ttttttgaaa tgtgtcattg ttttttctcc tcttttatgt
     5341 gacttagaga ggttagtaat aaatttccag ctgcctcttt catcttaagc atagtccttg
     5401 actagcactt tttaaaacaa taggccttaa ttgaaaacaa ctatcactat cagcgtttcc
     5461 aaaattcatc agttctacaa acaaaaatat tcagcaagga ttactttttc gattcatcag
     5521 ttaaaaagta ccttttttcg aaaaactatg ctatactttt aattcgttat ttacggcgga
     5581 aggagcaaac atgcgatgaa tgagaaacaa ctagaacaaa cgcacgtgga tgaaacgatc
     5641 gaactaatcc agatcgaaca aaatatgctg gatacaaaac aacaagaatt gactcaggaa
     5701 atgaatcatt cgacaaaaga cacagctaat cacaagattc gagctggttc caatgaagct
     5761 ttttatgaat ctgctgttga atatcgccaa catgagcagg aattgctttt gaaataccat
     5821 actcttgaag cacagcaaaa acgcctgcaa acgttgtctg tcatgaaaga tagtccttat
     5881 tttgcccgta tcgactttaa agaagaccgt gaaacagaaa cattatatct aggtatcgct
     5941 tctttaagag atctggcaga agaaacaatc gttattgact ggcgcgcacc aattgccaat
     6001 ctttattatg aaggtgagat tggtcccgca ttttatgaaa ctgaaacaga tagaatagat
     6061 gtcgatctgc agttgaaacg acaatttaaa atccaagaag gacgtatttt atctatggta
     6121 gatacgtcgg aagtaatcaa tgatgacttt ttgctagaga tattggatga atcatcaagc
     6181 aaccatatga aaaacatcgt ctctacgata caaaaagcac aaaatgcaat cattcgggac
     6241 acaacaagta aagtaatgct gattgaagga atcgccggaa gtggaaagac atccgctttg
     6301 cttcaacgta ttgcttttct gttgtatcat aatcgcaagt ggctcgaggc tgaaaacgtt
     6361 cttctatttt cacccaacca tttgttttct gattacatct cgactgtatt gccttcatta
     6421 ggtgaaagcg gcgtgcctac acagacattc aaaaattatt tggtgcagct gctgccagag
     6481 ttttctttac tagatgaaca gcaacaagaa agtggttttt tgctagggga aaaagatccg
     6541 atacaagtga tgaaaagcgg attgacctta gtcgatcaga ttgatcgcta tatccaaggc
     6601 atcacaaact acggtccctt gttccgtgat atgaaaataa atggccgtac gatcctatca
     6661 aaggaatcga ttcgccaatg gtataaagaa acaaatgaac aattaccttt acatcataga
     6721 ctttctctat tacaaacgaa gctcctgaaa aaattaggcg gtctgcaaaa agacgaaaca
     6781 cgtcagcaat gggtcaaaga tctggcggaa gaacagctcc aagaactata cgcaacagat
     6841 ccaaatcttg aatatacaga acaaaaggag cgttcattac gtaaaaaact ggccaaccaa
     6901 atcgtacgta aaagattccg tcgaatccat cgagggatca tgaattatca atttgtcaat
     6961 ataacgaaac aatatctcca ctttctacaa actgtgccac tttctcttct aacacgacat
     7021 aaaatcagcg agaccaggtg gcaggaacat ttgacaactg ttcgtacaca cttgcgtcaa
     7081 agagaattgc gtcaagagga tgctgtacta ttctttttac tgatgcgccg aacatatcca
     7141 gtgacggtta cacacaaagc tcgctatatc tttattgatg aaatgcagga tttcgcacca
     7201 tcacaagtgg cattgctgca agccttgttc ccaaaagcaa atttgacttt ctgtggagat
     7261 ctaaatcaaa atgtctttgg aaatgaaacg atcgttggat cattggaaga ccttttcgtg
     7321 gaccgtgaag tcactcgttt ccaattaacg acaagctatc gctccacaaa agaaatcact
     7381 gattttgcta atcattttat tgtgaaagaa catcaagtag aaacaacagc aagaaaagga
     7441 gaccttcctt tgctcatcca aggaacaaca gatgatatca aattgaattg gttagcagat
     7501 accattcgaa cgactcaggg aaaagcccgg tattggcgta cagcaatcat agctaaaaca
     7561 caagaagaat gcgaagctct ttatgctcaa ctgccagacg agttgaaaca acaagtccag
     7621 ctgattttaa atgagagtga ctttatgaag cgttccatta ttttacttcc agccttttta
     7681 gcaaaaggtc tggagtttga ccgagttttc ttgtgggatg tagatgcaga acatttttct
     7741 tctcagcaag acaaattgat cctttacacc atgtgtacac gtgcgatgca tgaattgacc
     7801 ctttttacct ccaataatga aagtcctttg ttaaacaata tggatatttc atcttatgag
     7861 aaaaaagtac tgtaagtaaa aagacaactg ctatcattaa tgttagcagt tgtcttttta
     7921 tttacaataa taattgcaga aaaagtaatt ca
//
LOCUS       DACDHJ010000110         7929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDHJ010000110 DACDHJ010000000
VERSION     DACDHJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.112"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            241..516
                     /locus_tag="IV223_001812"
     CDS             241..516
                     /locus_tag="IV223_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712094.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            529..2601
                     /locus_tag="IV223_001813"
     CDS             529..2601
                     /locus_tag="IV223_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8712095.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2766..4652
                     /locus_tag="IV223_001814"
     CDS             2766..4652
                     /locus_tag="IV223_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8712096.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4664..5611
                     /locus_tag="IV223_001815"
     CDS             4664..5611
                     /locus_tag="IV223_001815"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8712097.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5631..6158
                     /locus_tag="IV223_001816"
     CDS             5631..6158
                     /locus_tag="IV223_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8712098.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6221..6874)
                     /locus_tag="IV223_001817"
     CDS             complement(6221..6874)
                     /locus_tag="IV223_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8712099.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7006..7848
                     /locus_tag="IV223_001818"
     CDS             7006..7848
                     /locus_tag="IV223_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8712100.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPISIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         2708 a         1328 c         1683 g         2210 t
ORIGIN      
        1 atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa catccttttg
       61 tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg gaatagaatg
      121 aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac aatgaaatat
      181 ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga gatctggaaa
      241 atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg cgccctttcg
      301 atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt gatgtatcgt
      361 cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt acagtgtgaa
      421 ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa gaaaaaagaa
      481 caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat ggaatttgct
      541 gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag ccgcattttg
      601 ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg tctgacagag
      661 tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat tgcaccattg
      721 ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt cgggacaatt
      781 ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc gacgttatca
      841 ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc gttaggtccg
      901 acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa aaaggcgatg
      961 catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc cttccagttt
     1021 gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat gagtctagta
     1081 gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa acaaaaaata
     1141 attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct gatcgaactt
     1201 ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg aatcattgct
     1261 gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt gtttgatgca
     1321 gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact gaatgcatta
     1381 gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg gggagacggt
     1441 ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat gctttttgtg
     1501 atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa aaagttcaaa
     1561 aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac agtcagtttg
     1621 gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca gcggtctttg
     1681 cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat cattgtcttg
     1741 cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt gatgatttta
     1801 gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa tacaaaagaa
     1861 tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga tctgtacttg
     1921 gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact gattttgtcg
     1981 atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc taatggttat
     2041 cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca gattttgtct
     2101 tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct ccatccgaaa
     2161 atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga agatatccaa
     2221 gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt agataacttg
     2281 actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa acttgaagga
     2341 ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga tagtctgttt
     2401 acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga atacgaagtt
     2461 gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa tttgttagaa
     2521 tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt tcgagcaaag
     2581 tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa
     2641 aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca aaaagaacaa
     2701 ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca agataaggag
     2761 ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt gaaaaagatt
     2821 tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga
     2881 acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg
     2941 atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg
     3001 aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc
     3061 aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg
     3121 atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta
     3181 cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa
     3241 tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg
     3301 aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt
     3361 ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt
     3421 attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt
     3481 ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag
     3541 aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg
     3601 tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga
     3661 acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag
     3721 ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag
     3781 aacatttaga tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg
     3841 cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa
     3901 tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac
     3961 cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg
     4021 gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg
     4081 ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc
     4141 tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa
     4201 cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc
     4261 atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa
     4321 aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc
     4381 agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat
     4441 tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag
     4501 aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc actcgattac
     4561 aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt
     4621 gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct
     4681 tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg
     4741 cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt
     4801 aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg
     4861 agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt
     4921 tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag
     4981 atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga
     5041 gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg
     5101 ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt
     5161 gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc
     5221 ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca
     5281 agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc
     5341 gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc
     5401 ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca
     5461 aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga
     5521 aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca
     5581 tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg
     5641 caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt
     5701 tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac
     5761 gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga
     5821 cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc
     5881 ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc
     5941 aagaatttct gccattttgt acgatattat accgtacagt catccatcat gtattcgatg
     6001 gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag
     6061 gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga
     6121 atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct
     6181 gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag
     6241 agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc
     6301 ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca
     6361 tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga
     6421 gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca
     6481 acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag
     6541 tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca
     6601 aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca
     6661 atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat
     6721 aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc
     6781 ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata
     6841 tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa
     6901 ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt
     6961 taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa
     7021 atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt
     7081 gtcatgccga tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac
     7141 ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca
     7201 ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt
     7261 tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat
     7321 ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt
     7381 caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa
     7441 gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac actagataac
     7501 ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt tttgaacata
     7561 cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg gcgaggaaag
     7621 aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt gccaaatgtg
     7681 cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt caaagagggt
     7741 ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc ggttatcgct
     7801 acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc gaaaagagga
     7861 taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa attaggaaaa
     7921 taattgttt
//
LOCUS       DACDHJ010000111         7911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDHJ010000111 DACDHJ010000000
VERSION     DACDHJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.113"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..271)
                     /locus_tag="IV223_001819"
     CDS             complement(<1..271)
                     /locus_tag="IV223_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8712101.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCL"
     gene            complement(304..957)
                     /locus_tag="IV223_001820"
     CDS             complement(304..957)
                     /locus_tag="IV223_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8712102.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            complement(991..2277)
                     /locus_tag="IV223_001821"
     CDS             complement(991..2277)
                     /locus_tag="IV223_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAP8712103.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            complement(2300..3301)
                     /locus_tag="IV223_001822"
     CDS             complement(2300..3301)
                     /locus_tag="IV223_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP8712104.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            complement(3328..4095)
                     /locus_tag="IV223_001823"
     CDS             complement(3328..4095)
                     /locus_tag="IV223_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP8712105.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            complement(4901..6214)
                     /locus_tag="IV223_001824"
     CDS             complement(4901..6214)
                     /locus_tag="IV223_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8712106.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            complement(6243..6923)
                     /locus_tag="IV223_001825"
     CDS             complement(6243..6923)
                     /locus_tag="IV223_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8712107.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
BASE COUNT         2332 a         1901 c         1174 g         2504 t
ORIGIN      
        1 gtagacaata ccctattgct aaagcttcta ccaatttcat tggattattt cttatgtgta
       61 catcatgtat cacaggattt tcaaatttta cactattaaa tttctctcga aaaaaactga
      121 caccttcttc aacatactgc ggttcttcgg tgaaagacaa taccgtcaca tcgtagtctc
      181 tagataaaac actgataccg ttgtacgcac caatttttcc gccattatct agaggatatg
      241 gaaattgagt tgttataaat agtacattca taattttatc ctttcaatat gggcttagat
      301 attttatcta ttcccaaaaa atttatcttc caacattaga caccaacagt ggtaaattgg
      361 caaatggtac tcctgaatca tatatgtttc tgtttcagga gctttaacac aaacatctgc
      421 tacccttgca agttctccac ctttggctcc agttaagcca attactttca taccaagagc
      481 tctagcaaca acagttgcat ttatcacatt tttgctattg ccggatgtcg aaataccaag
      541 gaatacatcg ccaggtcttc caaatccaaa caactgctgt gcgaaaacac ctaggccatc
      601 aacatcatta atatacgcag tggaaagcgc ttcatgagca acgagaggaa tcgccatcaa
      661 accatattca agattcttag ctaattcaga accctttaca ggatcaaccg caatcaattt
      721 atctgcaaat tccctactaa cagggcgagg cgtcttaaag cttttcatca gctcacccgc
      781 aatatgctcg gaatcagcag cagatccgcc atttcctgct atcagcagct tgccatcatt
      841 taaatagcat tcctccataa tcaggtatcc gtcaacaata tcctgcttga ttacttcaag
      901 agtgggatag cgtttaatca gcaaatcaat gtgtttctgt aatctattgt ctatcattgt
      961 ttatactctc ctttcgtgat aaaacatccc ttagcagatc ttatctgcaa attcactcag
     1021 ggttgctatg gtcatatcct gtccatactc actctcgcca accaatgcag tcttacagcc
     1081 ggcggcttgg cccgctttaa tatcattctc gccatcgcca atcatccaag actgactcaa
     1141 atctatgttg aagtccgtag ctgcctgaat taacattccg ggctttggct ttctgcattc
     1201 acaatcgaat tttagctctg ggcgctcttc ctcatatccc ttatgtggat gatgcgggca
     1261 ataataaact gcatccaaat aagcgccctt atatccaaga agtgtctcca ttttgttatg
     1321 gatctcttcc aattcttcaa aagaaacttc acctcttgca attactggct gattcgttat
     1381 tacaatagcc agatatccag cggcattgat ctttttcact gcatcttcag tacccttgat
     1441 tagttcgaac tcatcaatat gacgcagaaa gccaacatat tgattaatcg ttccatcacg
     1501 atcaaggaaa acagctttct gcttattctg aagatttttg cccttcactc ttccagcctt
     1561 aaaatcttca cagacagcat aataacgatc aggggtaccc atgtctttta cgtattctgg
     1621 actatcatag cagaacattt ttccggttcc tgccaacggc tttaataact gacggtccaa
     1681 gtcaatctta ggtgtgttta tctcgacatc aagaacagca ggactaatca catggagtcc
     1741 cgcatttact ctgttacggt aataagttgg acgggaatct tcctttacaa gccatctgtc
     1801 tacagagcca tccttatctg caatgattag accgctgtca tatggatgac tgttagggtg
     1861 agtaaacaat gtcaccaagc cacccttttc acggtgatat gctacaaatc ggttgaaatc
     1921 aacgtcaaac atcgcatctg cattcagcag gaggaagtct tcggtcaaat tatcctttaa
     1981 acgaaacaat gcaccggcat ttcctaatgg ctctttctcc aaaaagtatt caattttaac
     2041 gtcaaagggc tgaccagtaa ctggtgaaat tccagaacca tcgccaaaat agttcataat
     2101 gatatcgcca agatggctaa cggtaagaat aatatctgtg aatccctggt ctctcagaca
     2161 ttcgatttcg tgctccaata ccggtttgtt ttcaatttta atcatgggct ttggaatatc
     2221 gcttgcaacc gatgagatgc gagttccctt accgcctgcc ataataacaa ctttcatatt
     2281 cttatcctcg ctgatttctt tattttggaa tatacatttc cggtgtgtaa tgaataacct
     2341 gtgttccacc gttttcaaat tcaaacggaa tatacatcaa attactcatt gccgaacgca
     2401 cagcattttg tttttcaggc tgaacataaa aaaccaagaa accgccgcca cctgctccga
     2461 gaagtttacc gcccaatgca ccagcagcca tgcccttttc ataaagttca tcgatacttc
     2521 cagtgctaat cgcaccaccg gtagcctttt tcaatctcca agtgagatca agcagtctgc
     2581 cgaaatcatc tagatctctg ttcttatcag tcagaaccgc ttctgcttca tccacaagct
     2641 catacatttt tttcagtctt actcttttgt cctctgtgcc gctgacatta tttgccttct
     2701 gaacatcaga ggaaaatctg gtgaatccag taaagaacat catcaagttg ttgttaagct
     2761 gctttttacg ctcaggcgta ataataatag gcaatacttc atagccatct gagttgaaat
     2821 tgattcggtt aaagccacca taagctgctg caatctgatc ttgccatccg cctgcttcat
     2881 tacaaaggac tctttcaaga taaattgctt tatcaacgag tttcttcttg tcagcgtact
     2941 tgcccttcaa agcatagaaa gcattcaaca tacctacggc aaaagaacta gatgtaccca
     3001 gaccagaacg tgcaggaaga tctgcttcat atgtaaggcg aatttcatgc atatctagca
     3061 ttttcatcgc attacgaatt gcaggatgct cgatatcttc aatatttgtt actctctcag
     3121 tacgagaata tgataactct gtgctgtaat caaaaaatct aggcaaatga cgcacattca
     3181 cataacagta cttatcaaaa gtagtggaaa gcactgcacc gccattttct cgaaaataat
     3241 cttccatatc cgttccgccg ccaaaaaaag acatgcgaaa aggtgttttt gtaataatca
     3301 tacttcattg tcctcattga atatagttta ttttttccga tattttagtg ctaatttaaa
     3361 gatttttaaa tcatcaacca aatatcgttt tgccattctt ttagggtcct gagtaattct
     3421 aaacagccac tcaaatccat aatctgacat ccattttggt gctcttttaa cattccctgc
     3481 ctcaaagtcc aaactagctc ctagaccaaa agacacaggt actcccaatc tatcacagtt
     3541 ataaaacata aatttttcct gtttagggca acccaaaccc acaataagca tatctggatt
     3601 tgctcgttta atttgggaat caattttaaa catttcatct tcgtcttttt cgaacccata
     3661 aggaggagag tatgttccta caacatttaa tcccggaaat cttttcatca ggttatctgc
     3721 cgctttagcc gcaacgccct ctgccgcacc taaaaaatac attgtgtatc ccttttctgc
     3781 agccatcttg cacagtaaag aaaatatatc ggatcccgat attttttctt ttattggtgt
     3841 cttataaaac tttgaaatcc aaatcaatgg tttaccatcc gtcaaaatta aagatgcgtt
     3901 tttataaaca tcttgcaact cttttcctgt ttccaactga acaatgtgat ccacattcgg
     3961 agttacaaca tatccgcatc tcctatcttg aatcattttt tcaacctcta ccaatacttc
     4021 acccattgta agattgtcaa tttctgtgtt cataaatctc attcgtgaca ttagatatcc
     4081 tccctctatc cccatctaat tttcagtaag tccgcatcgc tcaacactta acatcttcca
     4141 atattcgttg gcaaaaacat acaatatctt ttttacaaat ctaatgagtt tcataacacc
     4201 atttgccgac tcaacttgtc cgtcccccga tatacataaa agaacttgaa aagattcctc
     4261 ttttaccgag aacgaaaacc ccgtctttac ttggattctt tcgggctcac agtatttaca
     4321 tctacacagt atttcacgag agccaccagg ataaaaatga atgagcttat ttcttttctc
     4381 caatttcaaa gtggcttcca tattcattac ctgaatcgct ttttcaaagt acaattctca
     4441 cttattatca ttcttatcta ctcgatcata atcgtaaact cgataggtaa catttgagct
     4501 ttcctgtatt tcagccacta atactccagc gccaatacca tgaactgaca taaaaaagat
     4561 ctcccttgtg tacttcttct ttatataaat gtttcccaag tgatctgtcc atcacagcat
     4621 ttcgtaacat ttcttctgtt acatcgtact caaaagcata aatcaccccc cggtgtagct
     4681 tccaatacat accacaactc ggttttacca ttctgtcctt catatttgat atttccgtac
     4741 gtagttatcg tctagatgaa ctaagcattc ccacgtataa gcttaatggt gttaagtcaa
     4801 ttttttccaa attccgtctt aaaccgttct ccaccccaaa gataatcttt acccacgggt
     4861 tgaaatttta gtatctccat aataactacc tcgcgaactt ttagttcaaa gaactcttat
     4921 acttgtcagc gacgctaata gtttcaccaa tctgaacttc gataatatcc agatttgtca
     4981 cggcttcgat tgtatgttta catcctgcgg caatagtcac cacatcaccc gcacgaagaa
     5041 tctgcttcat accatctaca atcgctttac cctgtcctgc tacaacagtc caaacttcat
     5101 cgcgagaatt gtggtagtgg tagctcaact tctgtccggc ctgcatagaa actttaacag
     5161 tcatggagcc ttcctgaacg tccataacgg tatatgtacc ccaggatttt tctgcaaaca
     5221 ttacttccgt actgattttc tcaacatacg gcttcatata gccactgcgc tctttatcag
     5281 aaatcagaat tccatcgcca cttgctgcaa ttaccatatc tttgcagccc atagcaagaa
     5341 taggaatatt caactcattc acaatctgag tattaacaca ggtatcatcc attacagctt
     5401 tccccttaat attatcagcc ataacttcag ccatcatgtt ccaagttccg acatctttcc
     5461 aatccccgtt ataacgcatc acttgaatgc tcgtttcttt ttcgaccaca gcatagtcaa
     5521 agctgatctt ggttaaagtc tcatacttgg aatacaaatc acgataatct tcaaaatcca
     5581 ccattgcgtg agctttttcc aacaggtaac caagtttgaa cgcaaataca ccggcattcc
     5641 aaacagcatt ctgagctaaa tatttctctg ccgttgcttt gtcaggtttt tccttaaact
     5701 ccttaacagt acttacttcg ttagaacttt cagggatgat ataaccgtac ttttcactag
     5761 gatatgtagg tgcaataccc atcaaagtca aattagccgt attctgctgc accaacttct
     5821 caagggcttc aacgccttcg taataggaat tatctacata aggatcaaca ggacaaaccg
     5881 caacactttc atcatcacct acgccaagtt catcgtgaag atatgctgca gctaacaaaa
     5941 ttgcagggaa agtatctcta cgacaaggtt ctacacaaat cgaagcctta tctcccaact
     6001 gattatggat tgcactagcc tgagacttac ttgttgcaat agtgaccttt gcatctgggt
     6061 caacagttgt aatctggcga taaactcttt gaatcataga ttcatattcg ccattttcat
     6121 ccttaaacag tttgatgaat tgtttgctac gaatatcgtt ggacagcggc cataaccgcc
     6181 taccgctacc acccgataat aaaataatat tcacatattt caactcctaa tactatatat
     6241 aatcattcac catatccaaa taatacagcc tgtaaagtca agaagataat cttccagtcc
     6301 gtgataaagc ttctttcatt gatgtacttc acatcaagtt ccacccattc ttcaaaggac
     6361 agttcattac gatgtggagc aatctgccaa tagcaactaa gaccaggctg tacgaacaac
     6421 cgctgccttt cgtagtcacc gtattgttcc acctctctcg gtaaagcagg tctgggtcca
     6481 ataatactca tatcaccttt aagaatgttc cagagctgag gtaactcatc gatacttgtc
     6541 tttcgaataa atttaccaac tctcgtgatt cttgggtcat ctttaatctt gaagaccgga
     6601 ccatccatct cgttttgtgc caacaaggct tctaattttg cttcagcatc tacacacata
     6661 gatcggaatt tataaaactt gaacagcttt ccatttcttc caactctatc ctgcacaaaa
     6721 atcgggctgc caccgggatc gtcaatcaca atggcgagcg ctgtaattag catcacaggg
     6781 ctcaatgcaa tcaaagctag agccgagaag aagatatctt gtgctctgcg tccaatccaa
     6841 tagaatctat gtcccctaag attctcttcc ctatcaattg ttacgtcttt ctgaacacct
     6901 ttcttttctt ctataccaat catctttttc cattcgtccc tctattttta caagtctcat
     6961 catctgtaaa cgacttaaag gcctgtgatc atcgaccact ctatctgaaa cagttctcat
     7021 cgaaccatgt tcattcctac taaaagcagc caactccaac gccggcacat tcaattttca
     7081 tatctatctc ctcctcgttt aggggagaaa tacgagttta ggtcattttt attgacctcg
     7141 tttggggcta tattagaatt tcacattgaa atgtacttac attctcaaca ccatcatttt
     7201 ccaaaaagta ttatgtaggt acatattgac ctctaatccc accccaaagg caggtttgaa
     7261 attcaatgat tgagctattc tcaccccaaa gatgagttta tcgaacaatt tattgggcta
     7321 ttactacccc aaactgtcta tttacagcaa atttatgatt ttatacattg aaattttcca
     7381 atccgaaaaa cccaaactaa ttttctgcgt tgtagacatt ggtaaatact tgaacaactt
     7441 cacggctatc atcatcacat cctacgataa taattctaat tggataaaga ttatcatcaa
     7501 taatttactt ccacgtaaaa attctgaact aaataaaatc tattgataat attttttctt
     7561 ccattttttg tataccctac aggtatagtg aagttaatta tccccaatgt ctctgaagta
     7621 acagacatca ctaattcaat aacacattca ctaccatagt ttgtaggagc agaaatgaca
     7681 acaaactcag cctccttatg tgctgttttt ccatctatcc aaattttatt acataggatt
     7741 cactaaaatt ttaccgttta ttttttcgat tctttttttg aaccgtccca tgaacatacc
     7801 gattcaacaa gctaaattat aaaaattggt gtgctgtcat tattttttgg aaaatttatt
     7861 aatggctatc aatatacttt tgtatcatac taaatagctc gtcagttcct a
//
LOCUS       DACDHJ010000112         7822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDHJ010000112 DACDHJ010000000
VERSION     DACDHJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.114"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IV223_001826"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IV223_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8712108.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IV223_001827"
     CDS             complement(2203..4389)
                     /locus_tag="IV223_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8712109.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IV223_001828"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IV223_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8712110.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IV223_001829"
     CDS             complement(4611..5051)
                     /locus_tag="IV223_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8712111.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IV223_001830"
     CDS             complement(5125..5664)
                     /locus_tag="IV223_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP8712112.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="IV223_001831"
     CDS             complement(6279..7745)
                     /locus_tag="IV223_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8712113.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2125 a         1725 c         1370 g         2602 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tg
//
LOCUS       DACDHJ010000113         7816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDHJ010000113 DACDHJ010000000
VERSION     DACDHJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.115"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1483)
                     /locus_tag="IV223_001832"
     CDS             complement(<1..1483)
                     /locus_tag="IV223_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8712114.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYR"
     gene            complement(1677..3299)
                     /locus_tag="IV223_001833"
     CDS             complement(1677..3299)
                     /locus_tag="IV223_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8712115.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQMFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(3748..5061)
                     /locus_tag="IV223_001834"
     CDS             complement(3748..5061)
                     /locus_tag="IV223_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8712116.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(5306..5530)
                     /locus_tag="IV223_001835"
     CDS             complement(5306..5530)
                     /locus_tag="IV223_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712117.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(5550..5918)
                     /locus_tag="IV223_001836"
     CDS             complement(5550..5918)
                     /locus_tag="IV223_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8712118.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(6115..6825)
                     /locus_tag="IV223_001837"
     CDS             complement(6115..6825)
                     /locus_tag="IV223_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712119.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(6843..7340)
                     /locus_tag="IV223_001838"
     CDS             complement(6843..7340)
                     /locus_tag="IV223_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712120.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(7377..>7816)
                     /locus_tag="IV223_001839"
     CDS             complement(7377..>7816)
                     /locus_tag="IV223_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8712121.1"
                     /translation="RDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPGIIQEK
                     LAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFIIRENNY
                     SNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
BASE COUNT         2271 a         1686 c         1255 g         2604 t
ORIGIN      
        1 atctatattc aaggttttta ccattagact ggataatttg tttataatat tcaaaactat
       61 cttttttcaa tctcctaaag cttccgctcc catcattttg tctatccaca taaataaagc
      121 catatcgctt cctcatttcc cctgttcctg ccgatacgat atcgattggt ccccaccata
      181 agtaaccaat gatttggcaa ccatctttta ttgcctcagc aatttcctga agatgtattt
      241 ttagatactc acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta
      301 gtccaatgcc attttcaact atgaataatg gtaattcata acggtctgtc aattcattca
      361 tgacataacg aaaccctaga ggatcaattg gccaactcca aggttcggga gatttttcct
      421 tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc tacgcgcatt tctgcttctt
      481 ttttatagac agcgctacga taataactaa atgcaatata atctgccgtg ttagcttgca
      541 agcactgttc gtctccatct aacattactg gtgcacagtc ttcttccttc catgtcctgt
      601 aaatataagc tggataatgc cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt
      661 taaattgcag tgaaccaaat acattctctg gatcacagtc atatgcataa gtcgctaatg
      721 cagaaaagct acacataata cccatcttag catttggatc gattttcttt aataattgag
      781 tagcacgggc gttcgctaga aacatatgat gacaggcttg ataaatcact ttttttgtta
      841 gtgtagcaat gggttcaagt ttatctctag cagggaatgt aaccaatcca gcagctgcat
      901 aaggcatacg aaaagcatta ttgatctcat taaaggtcat ccaatatttc actttccctt
      961 ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt
     1021 gccacccccc atactctcct accaatgcta agggggtttc ataatgacta agagtaatta
     1081 ctggctcgat atcatatcga tttaatgtat tgattagtct gtcataaaat acgagaccag
     1141 cctcatttgg cttctgttcg tctcctcttg gaaatattcg tgcccatgag atgcttgttc
     1201 gaaaagcttt taaacctaac tccttcagta aacgaatatc ctcttcaaat cgatgataaa
     1261 aatcaatcgc ttcatgagaa agataatgga gtgattcgtc taaagcaatc tcccaaatct
     1321 ttttttcttc attccactga atactaggat gctggttcat gatcccgata agcgtatctg
     1381 tcacattagg taatttgccg tcttccaacc atgcaccttc acattgatta gcagctactg
     1441 ctccgcccca taaaaaattt tctggaaata tttcctgatc cataactatc tctcctcgct
     1501 atcaacaatt tcttacttga agtatagctc gattcatcaa aacaagatat tctaactatt
     1561 tcggcagaag agaagaaaaa aattgaatgc tactcttcat ttttaatatt tatatataac
     1621 aaatacataa gacgatcccc gaaagaaaac actcgttaac catctaagca aacgatttat
     1681 tttacttcca acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt
     1741 ttcagaggat aatttgacac aataaccctt aaagcttcac gtggaacagt gaacatctgc
     1801 ttttcactgg acatattaca aataacgagc cattgctggt cttcatatat gcgagaatag
     1861 gcaaagacat gctcaggcgt cttttccact aattcatagt ctccccagac gatgatgtca
     1921 tgttttctac gcaaatcgat caatttcttg tacatataaa aaacagaatc ttgatctgct
     1981 aacgcttctt ctgcattgat ttctttataa tttggattga ctcgaagcca tggaattcca
     2041 gtagtaaacc cagcatgttc acgggcgttc cattgcatag gcgtcctagc attgtctctt
     2101 cctttcgcat tgattgattc caagatttcc tcttttgtaa atccagaaga aatcctttcg
     2161 ttatacatat tgattgtctc aatatctttt gcttccgcga tatcatcgat tgggcaattc
     2221 gtcatgccta actcttctcc ttgatagata taaggggtgc ctttcatcat atggagcaag
     2281 atagcaaaca tcttggcact ttcttttcga tacacatcgt catttcccca acgagaaaca
     2341 attcgcggaa gatcgtgatt attccaaaat aaactattcc atccttcatc acctaaagat
     2401 gtttgccatt tggataatac ttttttcagt tcagagatac tcaagggttt caaatcccat
     2461 ttgtctttgc cttcttgctg atctaatcct atgtgttcga attggaagac catggataat
     2521 tcatttcttt taggattgga ataaagttta gcgatttcgg tagttgctcc ccatgtttcg
     2581 ccaacagtca tcagattttt gtcaccaaaa gttgcttgat tcatttcctg aagatactca
     2641 tgcagtttag gtccattact tgtgatttct ttatccggtt ctttgccaat catatcgatc
     2701 acgtccatac gaaagccccc aataccttta tcgatccaga aattcatcat ttgataaatc
     2761 tcttggcgaa cctgtttatt tttccaattc aaatcaggtt gacgcttgct atacaagtgc
     2821 agaaaatatt gccccgtttt ttcatccaac tgccaagcag aaccactgaa tattgagcgc
     2881 aatctattag gctcttttcc attgaccgga tcacgccaaa tataatagtc ccgataagga
     2941 ttttctttat ttttacgggc ctcaatgaac cacggatgtt catcagaagt atgattcacc
     3001 accagatcca tgatgatacg gatatttctt ttctttgctt cttcaatcag ttcttccatt
     3061 tcttccatcg taccgaattc tgccatgatt gcttgataat tactgatatc atacccattg
     3121 tcatcattcg gagactcgta tactggagaa agccaaattg catcaatacc taaatacgcc
     3181 agataatcca acctggaaat aatcccccgc aaatctccaa taccatcttg atttgaatct
     3241 tgaaaacttc ttggatagat ttgataaaca actgaatggt gccaccattt ttgttccatg
     3301 attttcctcc tataaatgga tgtttctatt ttttgcttaa tcatttattt tagtctctct
     3361 cactaaaaat tgtaacgctt tcaaatagct attgcacctt gttatcggta acagaataaa
     3421 acaaaaaaac agctgaggaa attccctcaa ctgttttttg aaaggattga tttatgcagg
     3481 gaagaatgct gatttcacaa cttctttata aacggtgttc actcagctgt cccttggtat
     3541 tagctcaaaa aacggcaaaa tatgaggaac tattttttcg tttttttctc taatactcag
     3601 gccagaacac tgatttcaca acctctttat aaacggtgtt cactcagctg tccctcggta
     3661 ttagctcaaa aaacggcaaa atataagaaa ctattttttc gtttttttct ctaatactca
     3721 ggccagaacg ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccac
     3781 aaatacaaag aacagaccat acatgattgg agatacttct tttcctcttt tagctgctaa
     3841 catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa
     3901 aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat
     3961 ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg
     4021 tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt
     4081 agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa
     4141 tgcaccaaca ggagaagtac caaataagga acccgcaagc atagctgttg aatcagaagc
     4201 taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa
     4261 tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac
     4321 ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt
     4381 caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc
     4441 taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa
     4501 gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc
     4561 taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa
     4621 agcgataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc
     4681 aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa
     4741 tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa
     4801 aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat
     4861 taaaacacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat
     4921 tccagaagca cctaatacag ttggatttac aaataagata taagccattg agataaatgt
     4981 tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta
     5041 ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc
     5101 gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa acaaaacata
     5161 agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat taaatccctt
     5221 tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa
     5281 agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct
     5341 gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac
     5401 ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg
     5461 ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca
     5521 ttagatccac tgtttttcct cctttttatt caatcagacg actcagcttt tcctataaca
     5581 aaataattgc cttcagggtc agaaaaattg aaagtcaaga tgccattgtt atctgtgatt
     5641 tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa
     5701 gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg
     5761 cctaattcta attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg
     5821 attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg
     5881 tcattgacat aaagcatcac acgtgttttt tgtatcatca tttgaccctt ctttctattt
     5941 gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg
     6001 tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact
     6061 ttttttacaa aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa
     6121 ttctgcttct gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg
     6181 atcgattttt tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt
     6241 gatggattca atgaccagca ccgctgcttg tgtttcttcc gtcagcattg ttctttgggc
     6301 ttcacgccag ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc
     6361 cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc
     6421 ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc
     6481 aaaagacaag gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg
     6541 ttcttgattt gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt
     6601 gaatgtggta aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc
     6661 tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc
     6721 actttctttt gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc
     6781 aggaaatact tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt
     6841 gattagaata attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa
     6901 acatgttcgc tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg
     6961 tgttcccaaa caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct
     7021 gtatctagta aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc
     7081 tcgatttcta aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc
     7141 aggtaacccg caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt
     7201 agctcttctg taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc
     7261 ggtgaattat cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag
     7321 tctacatctg aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa
     7381 tagatccttt tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact
     7441 ttatttaaaa gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact
     7501 gcattggagt agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc
     7561 ggatagagtt tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg
     7621 gattctaatt tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct
     7681 agtttttctt ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct
     7741 cttgttaaat cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc
     7801 attccgatgt ctcgaa
//
LOCUS       DACDHJ010000114         7773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDHJ010000114 DACDHJ010000000
VERSION     DACDHJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.116"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(239..3892)
                     /gene="rpoC"
                     /locus_tag="IV223_001840"
     CDS             complement(239..3892)
                     /gene="rpoC"
                     /locus_tag="IV223_001840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8712122.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3971..7588)
                     /gene="rpoB"
                     /locus_tag="IV223_001841"
     CDS             complement(3971..7588)
                     /gene="rpoB"
                     /locus_tag="IV223_001841"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8712123.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
BASE COUNT         1999 a         1749 c         1534 g         2491 t
ORIGIN      
        1 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
       61 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
      121 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
      181 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
      241 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
      301 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
      361 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
      421 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
      481 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
      541 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
      601 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
      661 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
      721 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
      781 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
      841 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
      901 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
      961 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
     1021 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
     1081 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
     1141 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
     1201 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
     1261 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
     1321 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
     1381 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
     1441 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
     1501 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
     1561 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
     1621 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
     1681 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
     1741 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
     1801 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
     1861 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
     1921 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
     1981 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
     2041 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
     2101 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
     2161 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
     2221 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
     2281 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
     2341 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
     2401 ctagaaccat atcttgggaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
     2461 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
     2521 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
     2581 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
     2641 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
     2701 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
     2761 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
     2821 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
     2881 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
     2941 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
     3001 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
     3061 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
     3121 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
     3181 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
     3241 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
     3301 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
     3361 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
     3421 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
     3481 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
     3541 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
     3601 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
     3661 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
     3721 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
     3781 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
     3841 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
     3901 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
     3961 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
     4021 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
     4081 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
     4141 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
     4201 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
     4261 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
     4321 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
     4381 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
     4441 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
     4501 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
     4561 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
     4621 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
     4681 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
     4741 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
     4801 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
     4861 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
     4921 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
     4981 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
     5041 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
     5101 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
     5161 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
     5221 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
     5281 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
     5341 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
     5401 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
     5461 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
     5521 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
     5581 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
     5641 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
     5701 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
     5761 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
     5821 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
     5881 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
     5941 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
     6001 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
     6061 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
     6121 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
     6181 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
     6241 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
     6301 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
     6361 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
     6421 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
     6481 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
     6541 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
     6601 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
     6661 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
     6721 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
     6781 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
     6841 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
     6901 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
     6961 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
     7021 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
     7081 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
     7141 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
     7201 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
     7261 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
     7321 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
     7381 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
     7441 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
     7501 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
     7561 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
     7621 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
     7681 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
     7741 tcgtgagctt ctacttttgt ttccttatat taa
//
LOCUS       DACDHJ010000115         7737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDHJ010000115 DACDHJ010000000
VERSION     DACDHJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.117"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            402..1940
                     /locus_tag="IV223_001842"
     CDS             402..1940
                     /locus_tag="IV223_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8712124.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1937..2902
                     /locus_tag="IV223_001843"
     CDS             1937..2902
                     /locus_tag="IV223_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712125.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2889..4211
                     /locus_tag="IV223_001844"
     CDS             2889..4211
                     /locus_tag="IV223_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8712126.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4274..4378
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4452..5195
                     /locus_tag="IV223_001845"
     CDS             4452..5195
                     /locus_tag="IV223_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8712127.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVILQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5210..6202
                     /locus_tag="IV223_001846"
     CDS             5210..6202
                     /locus_tag="IV223_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8712128.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6214..6951
                     /locus_tag="IV223_001847"
     CDS             6214..6951
                     /locus_tag="IV223_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712129.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6941..7663
                     /locus_tag="IV223_001848"
     CDS             6941..7663
                     /locus_tag="IV223_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8712130.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         2523 a         1324 c         1670 g         2220 t
ORIGIN      
        1 acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg
       61 atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac
      121 tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga
      181 ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa
      241 aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt
      301 tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa
      361 agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa
      421 cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc
      481 tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc
      541 ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac
      601 ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg
      661 cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa
      721 ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc
      781 tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag
      841 ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat
      901 ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt
      961 ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc
     1021 gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc
     1081 agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg
     1141 aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat
     1201 taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg
     1261 atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa
     1321 tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata
     1381 agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag
     1441 tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct
     1501 ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag
     1561 gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc
     1621 gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc
     1681 agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc
     1741 aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac
     1801 tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca
     1861 atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag
     1921 taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga
     1981 tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt
     2041 ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa
     2101 accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa
     2161 aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt
     2221 caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga
     2281 ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat
     2341 gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc
     2401 aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa
     2461 ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt
     2521 tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg
     2581 agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt
     2641 tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt
     2701 caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac
     2761 gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa
     2821 gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga
     2881 ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg
     2941 ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg
     3001 gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg
     3061 cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact
     3121 gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa
     3181 aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt
     3241 catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc
     3301 aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt
     3361 tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg
     3421 agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac
     3481 ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat
     3541 ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct
     3601 atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga
     3661 gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg
     3721 gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata
     3781 ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag
     3841 ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg
     3901 tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag
     3961 atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag
     4021 ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta
     4081 gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta
     4141 aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt
     4201 tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac
     4261 tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa
     4321 cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg
     4381 aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag
     4441 gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta
     4501 tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg
     4561 ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat
     4621 taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg
     4681 caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg attttacaaa
     4741 tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc
     4801 caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata
     4861 cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag
     4921 ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa
     4981 aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag
     5041 caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg
     5101 ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga
     5161 aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt
     5221 atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag
     5281 tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa
     5341 tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt
     5401 aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga
     5461 caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca
     5521 gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac
     5581 cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg
     5641 gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt
     5701 ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc
     5761 agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga
     5821 actaaactat atcccattga aagaattaga tgaacggtta gattactata ctccggtaat
     5881 tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc
     5941 cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca
     6001 aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa
     6061 aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta
     6121 tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt
     6181 cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat
     6241 gtagtcattg aaaacctgaa agtattagaa gacatccatt tcactatccc taaagatacc
     6301 tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca
     6361 ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc
     6421 catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat
     6481 caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg
     6541 gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct
     6601 ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg
     6661 cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg
     6721 atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa
     6781 gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt
     6841 gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag
     6901 ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg
     6961 tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat
     7021 catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac
     7081 tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt
     7141 tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac
     7201 gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat
     7261 aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat
     7321 ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga
     7381 ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt
     7441 ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca
     7501 tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga
     7561 agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa
     7621 tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca
     7681 actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt gattaat
//
LOCUS       DACDHJ010000116         7728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDHJ010000116 DACDHJ010000000
VERSION     DACDHJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.118"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..1664
                     /locus_tag="IV223_001849"
     CDS             24..1664
                     /locus_tag="IV223_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8712131.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1818..3140
                     /locus_tag="IV223_001850"
     CDS             1818..3140
                     /locus_tag="IV223_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8712132.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3202..3633)
                     /locus_tag="IV223_001851"
     CDS             complement(3202..3633)
                     /locus_tag="IV223_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712133.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3697..4533)
                     /locus_tag="IV223_001852"
     CDS             complement(3697..4533)
                     /locus_tag="IV223_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8712134.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4723..5025)
                     /locus_tag="IV223_001853"
     CDS             complement(4723..5025)
                     /locus_tag="IV223_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8712135.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5051..5482)
                     /gene="ruvX"
                     /locus_tag="IV223_001854"
     CDS             complement(5051..5482)
                     /gene="ruvX"
                     /locus_tag="IV223_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8712136.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5479..5745)
                     /locus_tag="IV223_001855"
     CDS             complement(5479..5745)
                     /locus_tag="IV223_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8712137.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(5999..6724)
                     /locus_tag="IV223_001856"
     CDS             complement(5999..6724)
                     /locus_tag="IV223_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8712138.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7090..7160)
                     /locus_tag="IV223_001857"
     tRNA            complement(7090..7160)
                     /locus_tag="IV223_001857"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7126..7128),aa:Gly,seq:ccc)
     gene            7277..>7728
                     /locus_tag="IV223_001858"
     CDS             7277..>7728
                     /locus_tag="IV223_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8712139.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLG"
BASE COUNT         2279 a         1586 c         1413 g         2450 t
ORIGIN      
        1 ttaaaactta ggaggaatca ttcatgctta cagggctaaa cttattaaat aatccttttc
       61 taaataaagg aacagctttt acaaaagaag aacgggcaaa atacggaatc actggtatgc
      121 ttccaagtac tgtccaaaca ttggaacaac aatctgtcca agcttacggt caatacttaa
      181 gcaaacagac cgatttggaa aaacgtatct tcttaatgaa tctattcaat acgaaccgga
      241 cattgtttta taaattgatg ggcgaacatt tagttgaatt catgcctgtc gtttatgatc
      301 ctgtcgttgc ggattcaatc gaacaataca acgaaatttt cttgaaacca caagatgctg
      361 tatttctttc aatcgacgaa ccagaaaata tcaaagcaag tttgaaaaac gcagcagatg
      421 gacgcgacat tcgtttgatc gttgtgacag atgcagaagg aatcttaggt atgggtgact
      481 ggggtgtgaa cggtgttgac atcgctatcg gtaaattgat ggtctacact gccgcggctg
      541 gtatcaatcc tgcacaagtc ttgcctgtat caatcgacgc tggtactaat aacgaagaat
      601 tattgaataa tccattatac ttagggaatc gtcatgctcg tgtagaaggc gaaacttact
      661 acgaattcat tgatcaattc gttcaatcag ctactgaatt attcccagaa ttgttattac
      721 attgggaaga ttttggacga ggaaatgctg caacgatctt agaaaaatac gaagataaaa
      781 tcactacttt caatgatgat atccaaggca caggaatcgt tgtactagct ggtgtattag
      841 gtggattgaa tatatcaggt gagcaattga aagatcaaac gatcttgaca tttggtgctg
      901 gtactgctgg tgtaggtatc gcaaacatcc ttttagatga aatgatccgt caaggtgtac
      961 ctgaagaaga agcacgccaa catttctatc aagtagataa acagggttta ttgtttgaag
     1021 atacaaaagg attgactcct ggtcaaatcc cattcgctcg taaacgctca gaattcacta
     1081 atgctgatga attgacaaac ttagaagcag ttgtaaaagc aatccatcca acgatcatga
     1141 tcggtacttc tacccaacct ggagcattca ctgaagaaat cgtgaaagaa atggcggctc
     1201 ataccccacg tccaatcatc atgcctttgt ctaatccaac aaaattggca gaagcaaaag
     1261 caaaagattt gattgaatgg acggacggaa aagctttagt tggaacaggt atccctgctg
     1321 ctgatgtgga atataacggt gtaacttacc aaatcggtca agcaaataat gcgttgatgt
     1381 acccaggttt agggcttggt ttgattgctt caacagctac acgagtaaat gctgaaatca
     1441 tttctcaagc tagtcgagca ttaggcggaa tcgttgatgt aacaaaacca ggagcagcta
     1501 tcttgcctcc agtagcaaaa atcactgaat tctctcaaac gattgctgaa actgtcgcaa
     1561 aatcagttgt tgcacaaaaa ttgaatcgtg aagaaatcac ggatatcaaa gaagctgttg
     1621 aatctgctaa atgggttcct gaatataaat ctttagaaga ttaagattgt tatagtaaaa
     1681 gaacatacag aaactttgta tgacgtctaa aaagaaaaaa gcgactgcgg caaaacagca
     1741 gccagccaac tactgctctt ttgccgcttc tctttggctt ttttcatacg atacagaata
     1801 agaaaggatc tgtaaaaatg gcacaaaaaa caacaaccgc aactgcagtt gaggaaaaga
     1861 aaaaagaagg gttttggtca acaaaaatta ccggcgtaag tttgcccctt tatttgatca
     1921 tggtccttgt cctgatcatt gcaatggctt taggaaaatt accgacgaat atgattggcc
     1981 caatcgctgt tttggtcatt ttcggtaatt tatttcattt tatcggaaca aaaatcccta
     2041 tagtcaaaag ttatttaggt ggtggctccg tttttgcgat ctttgcctct gccgcgattg
     2101 ctacatttgg gatcttacct gagtatgtag taaaatccac agaaaacttt gtcagcaata
     2161 tgggcttttt agacttttat attgctgcct tgattactgg tagcatcctt ggtatgaacc
     2221 gtaatctatt gatgaaagct tctgttcgtt ttattcccgt tgcacttatt tcaatggtcg
     2281 tctccttttt tgccgtagga ctcgtaggta tgcttatcgg aaatggtttt gcaaattccg
     2341 ttttatatat ctcattgccc gcaatggctg gtggtgttgg agccggagct gtacctttat
     2401 caacgattta tgcaaatgtt ttaggtacgg acgcttcttc tatcatttcc cgcctgattc
     2461 ctgcgacagc aatgacaaat gtcctagcta tcattggtgc agcgttagtt gctcgtgcgg
     2521 gacaatcaat gccaaaattc aacggtaatg gtaaattaat ggaaaaaggc gaccttggcg
     2581 atggaaaacc tagaaatgta aaaccttcca ttccacaatt aggtgtcggt ttgatggtat
     2641 cattgacttt cttcatctta ggacaaatct gtaactattt tgttcctaaa gtccatgctt
     2701 acgcattcat gatcattatc gttgttatct gtaaaatcgc aaacctatta ccagaatatt
     2761 acgaagatgc agctatcatg ttcaacaact tgattgtcaa aaacttgaca gcagctgttc
     2821 ttgccggtat cggtattgcg ttattgaatc taaatgtatt ggcttctgct ttgacatggc
     2881 aatttgttgt cttatgtttg acaagtgtta tcgcgatttc agtagtttca ggatttgtcg
     2941 gtcgtttgtt tggtttatat ccaatcgaat caactattac tgctggctta tgcaacaact
     3001 cgatgggtgg tacaggaaat gtagctgtct tatctgcagc taatcggatg gaacttattg
     3061 cttttgctca aatgggcaat cggcttggtg gtgccatcgt tttgatcatc tcaggtttct
     3121 tgatgcaatt actttcttag tcaaaaaaaa tacctgatca tcaagtatcg gcttgatgac
     3181 caggtatttt ttgataaaaa tttacaggat tttcaaaaat cttttttgca ttttatcgga
     3241 atgataaccg atgcgttcat aaaatcggtg tgcttctact ctgtattctg cagaattaag
     3301 tcttattgct gatattcctc gtcttttcgc ttcttctacg ccagtcatca aggcctttcc
     3361 gattcctctt ttttcagcat gtttagacac agcaagtgcc ataatattga acatcggatc
     3421 gctgaaaatc gattcatagg actccgcttg tacatagcca gctgcttttt tagaagtagg
     3481 atcttcatag accaagaaaa aatttttttc tggctccttc aacagtttct tcatttgtct
     3541 caccgtaacg gttaatggta catcatatcc taattgttcc gcattgattt ctcgtagtgc
     3601 ttctgcatct cctaaacgca tttcccgcaa catccaaatc accagccaat ttttattttt
     3661 ttaaatgaat ttttttatca cagtatactc cttttttcac ttaaaaaaag acttttcagt
     3721 gattttgact tgcttgaatc cagatccttc taatgtttgc aagatgacga aagcattgta
     3781 aagattgatc aaaagaacca cgatgacaat cgcttgaaaa ataaaggtca ctttttttgg
     3841 agacaaaacc ctgctaaatc ttgctcccca caaacctcca ataacagcag cggggataac
     3901 ataaaacaac atagataaat caaagcgcat aaagctactt gttaaagcaa tcgttaccaa
     3961 ttttgccagt tgagaaaaga aaatcgtaga tagagaatat agtgttgctt cttttattgg
     4021 caatgcaaac atcagcatca acaaagaaac attgactggt ccaccaccaa tacctaaaaa
     4081 actagctaaa aagcccagaa cccccccaca tataaggtac caagtccatg acgtcaaatg
     4141 aaattttggc tgatgatgct tggtgtagaa gaacgcaaaa accaaggtca ataccgtcaa
     4201 aaaaatttgg atcagctgca catgcttctc gttttcaaat aactgtaaaa aaacttcgaa
     4261 tacaatgttg ccgactactc ctccaacaac agctccacta gatacccaac cgacaatctg
     4321 ccaattcagt gatttcccgc ttgatacttg tcgcacagtc gaaacaatgg acatcgtgaa
     4381 taccgcaact gttgagtaaa aagaaatccc tgcaacagaa tgggcgccga taagatcaag
     4441 aatcggtttg atcaacacgc ctccacccat cccggaaata gcgccaactg tgttcgccag
     4501 aacgatcacc acaaaataaa tcagaccaat catcttgtat ttcctttctc ttgtttatat
     4561 cttttagcga ttgttactct tttcttttat tatacagtaa aagaaacaaa cgaggcttcg
     4621 aaagcttttc tactttttaa ggaaatttag tgctaaacga aaaaaccggg ctctactttt
     4681 cagagagtcc agcttttctt acaccttatc gattcttttg ttctattctt cttccgccat
     4741 gaatgtattg aatacttctt cgatcatatc ccattctgcg tctgtttcga tttgttctaa
     4801 ctcgccttct gttccatctt cgttttcgat gtatgcataa gcttgtaatt ctacatcttc
     4861 atcttctgaa acaccagctg gatataaaag aacatagtta cgtccaaatt cttcttgtcc
     4921 gtcaactgtt agcaagattt cgtataatgt ttcatttcct tggtcatcca ccaatgtgat
     4981 gtgttcgtgt ccttcgtgtt cgtgggaatg gttatgttct gtcataagat tacctctttt
     5041 ctttttatta ttaaaataat tgcttacctg agccgtctaa ataattttgt aaaatcatca
     5101 cagctgctaa tttatcgatg actttttttc gtttcgctct tgaagtattt gcctgctcta
     5161 ctagcatacg ttctgcctga acagtggtta gtcgttcatc ttgataatca accggcaagg
     5221 agaatagttc tttgatcttc tctccatatg acatggaagc ttctgccctt ggtccgatgg
     5281 aattgttcat attcttaggt aatccaacta caaaacggtc tacttcgtac tgcgccacta
     5341 attccttcaa ccgatcaaaa ccaaactctt cttctgcctc attgattcga atgatttcta
     5401 ctccttgagc tgtccagcca aagggatcgc tcacagcaac gcctactgtc ttagaaccga
     5461 catccaagcc cattactctc ataaattcac tccatgattt gagaggtagt ctttgacaat
     5521 tacttccatg atttcatcgc gttcgtgacg gcgaatcaaa tttcgtgcat cacgataacg
     5581 aggaatgtag gccgggtctc cagaaagcaa gtagccgaca atctgattga ttggattgta
     5641 gcctttttct tccaaagccc gataaacaat cgctaatgtt tcgctcactt cttttttgcg
     5701 actatcgtca aaaccaaaac gtacggtttc atctgtaaaa cccatgattc tacacctctt
     5761 tcccgcaagg taattccagc ttaccttcta cacccttatt ttactcgaag aaagggaaaa
     5821 ctacaaacaa atattccaaa cttctgaata aattaagaaa cacgatttgt ctgatttttc
     5881 cttttttctg tggctttttc aaaagccctc tttccataaa gtatacgcta tatttctttg
     5941 aaagtatgtt tttacacaat tttttcataa agttcataaa aaattcgaat tgctgctact
     6001 aatttttcgt tttcgttgct tgccctgtct cataattgat ggtgtagcca tcttgaacat
     6061 tgtagataaa gacattgaat tcaatttttt tgtcttcaat gctttctgct tccaactgca
     6121 cacctcttgc taccagttca tttccctcga aatgaggagt gacacgatag cgtacatgat
     6181 gatttgtttc cttgatatat gccgcaatat cattttcgtg ggcaagcatg tacggagcat
     6241 tcaacgaacg tgtccctgtc attaagtttt taatgttatt attttgacca gtcaactgga
     6301 aaccaatcaa atggctgcgg ttgtataacc attgtccgtc acttaatttc ttttgtttcc
     6361 aacctgtcgg atcaatatac aaaggttctc tcttttcagt aggaaacaag tcttttccca
     6421 acatcgcatt ggcttgtcct actctgttca atttgtctat atttgaatag ctttcccatg
     6481 tccctttgga aagactaaga tcctcttttg taaactccgg ctgattccca ttgatttcta
     6541 tttcttgatg ttttccatca tattctggca catccgctac ttgagaagct tgcggtgtgg
     6601 aatcaaaaat ggttgtaatt tgttggaaga tatctgaatc tttttgtgtg gtggaaatac
     6661 cgagtgcccc tgcaatcaca gcagcgacga tccccacgat cgccttggtt gtttttttgc
     6721 tcatttcttt actcctttac taacagttcc ctaaatctta acatatgttc ccctataaat
     6781 aaatactgga aagaaataga aacagtaaaa aaataaaacc agtttttaga cggcttaaga
     6841 atacaaaaaa agagacggat tcatcgtctc tactcaatca caaaacacta tttacaaggt
     6901 atttattatc attcaaaaaa tttttcttaa tactcaaaac taaacggctt ctgtacgacc
     6961 tcttggtaaa cagtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagtcaaaaa
     7021 cataagaagc ttttttgctt gactattctt attacttgat gcagggcagc tttttcacag
     7081 cctcttttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa gctgggattt
     7141 taccactaaa ctacacccgc gatacaaaaa cagtatacgc ctctttcact ccttcctcaa
     7201 gtttttttat tctttacgct tccgatatgt tttgtagtaa aataaaacta atcaatcaat
     7261 tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca cttgtttact
     7321 cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag atacgatgag
     7381 tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc aaaccggtct
     7441 ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca accgttcgat
     7501 taatttagaa aaagtagtaa aagtaaatga tctttcacgt aaatatgttg cttcagagct
     7561 aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt ctttctttcc
     7621 tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg tgttatttgg
     7681 cggaaagatg accgatttac ctgtgacttt agtcattgga ggattggg
//
LOCUS       DACDHJ010000117         7662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDHJ010000117 DACDHJ010000000
VERSION     DACDHJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.119"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(86..571)
                     /locus_tag="IV223_001859"
     CDS             complement(86..571)
                     /locus_tag="IV223_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8712140.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1051..1692
                     /locus_tag="IV223_001860"
     CDS             1051..1692
                     /locus_tag="IV223_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8712141.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1711..2346
                     /locus_tag="IV223_001861"
     CDS             1711..2346
                     /locus_tag="IV223_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8712142.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2364..3179
                     /locus_tag="IV223_001862"
     CDS             2364..3179
                     /locus_tag="IV223_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8712143.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3498..4934
                     /locus_tag="IV223_001863"
     CDS             3498..4934
                     /locus_tag="IV223_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8712144.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5123..7570
                     /locus_tag="IV223_001864"
     CDS             5123..7570
                     /locus_tag="IV223_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8712145.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         2565 a         1297 c         1716 g         2084 t
ORIGIN      
        1 gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga cgcttgaatt
       61 gggaaataag aaaactactc tatcattaca aattttcata aactggcaga tgttctaagt
      121 aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta ttgatatttg
      181 gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact tggatttttt
      241 tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg cctaggaggt
      301 caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt tcagctagtt
      361 gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt tttccttttc
      421 ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg acatttttga
      481 ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt tttagtagtt
      541 cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt tttattagtg
      601 tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt aaagttaaaa
      661 caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc tggttggtgg
      721 aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa gcgtatagta
      781 ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga taaggctggc
      841 ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc
      901 ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca
      961 aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat ttgccaagag
     1021 gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt tatgccgcaa
     1081 ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat tggctcatta
     1141 ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct acgctacttg
     1201 cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact gatttttatt
     1261 ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc
     1321 tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat acaatcgatt
     1381 cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca aacggtgacg
     1441 aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa tgaagttatc
     1501 aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt aatgcgtgct
     1561 gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc
     1621 tatcttacat tgactggtat tctaacaatc gtaatgaaac aagttgaagg aaaattaaat
     1681 tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt gaacaaagca
     1741 tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg gaaaacatta
     1801 gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt ggctggctta
     1861 gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc attcgagctt
     1921 aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt tccacatctg
     1981 aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac aaaagaagaa
     2041 tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt attgaatatc
     2101 tatccttatc agctttctgg cggacaaaaa cagcgggtag cgattgccag agcattggct
     2161 atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc
     2221 cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca aattattgtc
     2281 acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt
     2341 cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct ttgatcgcag
     2401 gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat tgggcgacta
     2461 tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct atgggattta
     2521 gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca gtctttgaac
     2581 aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa tttgaactga
     2641 acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca agagaaaaga
     2701 aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca cctaaagaat
     2761 caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa aatgggtcat
     2821 ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc aaaaaccaag
     2881 atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc agacgggtcg
     2941 atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga aacgaactag
     3001 aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt ggtgcacgca
     3061 agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta gagaaaacag
     3121 gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg agaaattaac
     3181 aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa acagtgttct
     3241 cctgccaact ccttgtgact cggagctgaa tggcaggctc acaacctctt ggtaaacggt
     3301 gttcaaaagc cagtttttcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt
     3361 tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa
     3421 ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat
     3481 aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt
     3541 ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag
     3601 aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg
     3661 atttggcagt agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag
     3721 gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag
     3781 aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg
     3841 atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat
     3901 ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat
     3961 ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca
     4021 cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt
     4081 atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt
     4141 tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca
     4201 gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga
     4261 tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag
     4321 gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt
     4381 ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta aacggagttg
     4441 gtgcaggaaa aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg
     4501 gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg
     4561 acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac
     4621 tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg
     4681 acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg
     4741 atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga
     4801 acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat
     4861 ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg
     4921 gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct
     4981 tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag
     5041 aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac
     5101 ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa
     5161 gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg
     5221 gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt
     5281 gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt
     5341 agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg
     5401 acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt
     5461 atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc
     5521 cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat
     5581 cctttatcat tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct
     5641 attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg
     5701 tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc
     5761 tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag
     5821 gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc
     5881 ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc
     5941 ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct
     6001 agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga
     6061 gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca
     6121 agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga
     6181 tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg
     6241 tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca
     6301 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
     6361 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
     6421 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
     6481 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
     6541 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
     6601 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
     6661 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
     6721 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
     6781 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
     6841 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
     6901 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
     6961 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
     7021 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
     7081 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
     7141 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
     7201 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
     7261 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     7321 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     7381 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
     7441 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
     7501 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
     7561 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
     7621 catgatgata ccaaaagaaa aaatggtttt tttccatctt tt
//
LOCUS       DACDHJ010000118         7627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDHJ010000118 DACDHJ010000000
VERSION     DACDHJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.120"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IV223_001865"
     CDS             complement(16..708)
                     /locus_tag="IV223_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8712146.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            793..969
                     /locus_tag="IV223_001866"
                     /pseudo
     CDS             793..969
                     /locus_tag="IV223_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="IV223_001867"
     CDS             complement(1145..1714)
                     /locus_tag="IV223_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8712147.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IV223_001868"
     CDS             complement(1788..2111)
                     /locus_tag="IV223_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712148.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IV223_001869"
     CDS             complement(2265..3077)
                     /locus_tag="IV223_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712149.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IV223_001870"
     CDS             3431..4279
                     /locus_tag="IV223_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8712150.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IV223_001871"
     CDS             4424..5365
                     /locus_tag="IV223_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8712151.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IV223_001872"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IV223_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2273 a         1512 c         1372 g         2470 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacct
//
LOCUS       DACDHJ010000119         7618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDHJ010000119 DACDHJ010000000
VERSION     DACDHJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.121"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            10..2337
                     /locus_tag="IV223_001873"
     CDS             10..2337
                     /locus_tag="IV223_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8712152.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2463..2792
                     /locus_tag="IV223_001874"
     CDS             2463..2792
                     /locus_tag="IV223_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8712153.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2832..3836
                     /locus_tag="IV223_001875"
     CDS             2832..3836
                     /locus_tag="IV223_001875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8712154.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3833..5262
                     /locus_tag="IV223_001876"
                     /pseudo
     CDS             3833..5262
                     /locus_tag="IV223_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5468..6640
                     /locus_tag="IV223_001877"
     CDS             5468..6640
                     /locus_tag="IV223_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8712155.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6802..7257)
                     /locus_tag="IV223_001878"
     CDS             complement(6802..7257)
                     /locus_tag="IV223_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712156.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2573 a         1440 c         1519 g         2086 t
ORIGIN      
        1 agaggaggga tgaaaatgat taagaaaact caaaataaaa tccaacgtat atgtcaaaaa
       61 cattttgttt ctagactcaa agctgtatct aaaagaaaat acttacatcc cattatgatt
      121 tcgatcatct tgatttttac tttcttaggg agcttatcaa gtatttatgt aaacgtttat
      181 acagaaacgt caaattttgc taaccaaacg acagaggaga caaatagtcc accctattct
      241 gaaatacagt ccatagaaag caccaatcag tctagcaaca acgagacaac ctcaaccaca
      301 tcctcgactc aactgattga tccgacagaa aaagaaatca cagcagcaaa agcagcaggg
      361 gagaaagctt attcagaaac aggtagaagc cagacattta gtactgttgc ggccactgcg
      421 aatttcaacg gacataccat accagatgga tatacagtga tatatgccaa agatggagtc
      481 attgttgcgg ttaacggatc gatgagtgcg gataaaactg cgttacaagg cgtaaccaaa
      541 gcaatcgctt ttattgataa ccgtccagat atccataatc tgtttattgg ctccacagta
      601 aatggtacta ctatcacatt cccaaaaagt atctatatgg gggtggctac cgctggaaat
      661 acgatcgatt cagttacacc caatgcggat aaaagttatt cttttacacc ctcaacatgg
      721 ggagggcttc ctgccggagg aggtatgagt actttttcaa tcggtataca gttagcaaat
      781 gatattccat tcacggggta ctcgatcaaa aatgggcaga tcgttgatgg taagggaaat
      841 gtttcaaatt ttccaaccta tcaagcagca acggttcagg tcaatgcgaa tcggacagga
      901 actttttctt atacagtcaa tggtggagca gcaaaaatcg gaacgtacgg gactacatta
      961 tctggaatcc atgtgggtga caaagtggtt atcactccaa cagcagtaac gggctattct
     1021 tttgcacaaa caaacggttc tttcacagcc ctagaaagta acgtcgatac ggtgacctat
     1081 acaggcaaac aaacaccaat cactattaag tatcaagact attttggaca gatgattgct
     1141 ccttctaaaa taatgaatct tacttacgga agtgctgcac aagacctgac gactaatacg
     1201 cctactatca acggatatac ctttacagct gtttcagcga cagaaacaaa gaaccaatca
     1261 gcaatgagtg ttttagctag tttagataca aatggaaatg taatcgtaaa ggacgcaaat
     1321 ggaaaacaga tttctgaagt catcctatat tataagacaa atgcaacagt atcaatcaat
     1381 gcaaatggca gtaagtacta tgatggtctt tctgttgtac cgatcgttaa atatgccttt
     1441 aatgataaaa cagaatcaac atccctttta aacaatgatc tcagtattga tcaaatcaca
     1501 tggaatccaa ctgatttcaa agcgatgaat gaaaagggag ttgaagtaac tgcgcctact
     1561 gaaattggaa cctatacgtc ttggcaattg acaaaaagcg gactagctaa actagctgcc
     1621 agaacaaact atttatttac tatcgtgcag acatctgatg tctatacgat taagcaaatc
     1681 agcggtgcgg tagagctcag tggcagtaag acatatgatg gaaaagcagg tgtcccgtca
     1741 atccatgtca agctagcaga gggtgtcact agttcgctta ctccagtaca agtggcactt
     1801 agcagtatgg attacacagc agatgcacaa tcagcaaaaa atatggttaa tgctggcagc
     1861 tatacaatta aactaacgaa tagcggaata gacaaagtca aagcagcaaa tcccaagttg
     1921 tcatttactg atatagccaa cacatcagga acttatacca tctataaagc taacgcagtg
     1981 attactgtcg atgacgctgc ctttaattat gatgcccaaa gtcactcgat ttcggttgga
     2041 aatctccacg ttaccggtgt tgtcttaggc gaatcattgg attataccct aacagttaac
     2101 agccggacag atattggatc acaaatagta acgattagtt taggcgaagg aagtgtaaac
     2161 aataattata cgattatcgc aacagatacc gctaagttga cgatcaatcc gactctcttg
     2221 cttccttcga caggtgggat aggtctactg ccctttgtct ttttaggact gatctttatt
     2281 ttttctggct tcttttattt tatccatcgt aagaaaaaag caggtgaaca gcgatgaaaa
     2341 aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt ttttatccta ctggtctttt
     2401 ttataggttc tccgactgtg tcagctagag aatcacctat tacatttact ttgcataatc
     2461 gaatgttcga aagcgataag atgccaagta tcagtcaaaa cgacggaata aatatcccgg
     2521 ataactttca aggagtaaaa gggttgaacg gcgttacttt cgctatatat gatgtctcgg
     2581 atgaatttta caagctgcgg tcagaaggaa gtagtgtgga agacgcacaa agaaaactag
     2641 cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt gacaaaaaca gtagacgagg
     2701 aagagggcat tgcttctttt agtgcatcgg acaaagatga gcaaggtcgt gacgctgttt
     2761 accgatttgc ggaaataaag acttcagatt aagtaaaaga aaaatcagca ccatttgtcg
     2821 ttgtgcttcc tgtgacagat tcatctaatc ataaattgac gaccatccac ttgtatccaa
     2881 agagcgaaca aaaaatacct gctgcactca cattaacaaa aacagtagag aataagcaga
     2941 cagattttgc agatggggat aaagttcctt atctcatcac cgtaacaatt cctgagaaca
     3001 tcaacgagat aggaacctat acgatcaaag atacagctga tcctcagtta tggctagaac
     3061 cagaaacgat cgattttttg attggtggag acaagattca tacatttcac acacaaaaaa
     3121 caaaacatgg ttttgtctta tcagattctg gaaaagatct tagttcgttt gctgggaaaa
     3181 aattaacgat ccgttaccaa atgacattga aagaaaacag tgaaaaaacg acatttgata
     3241 acgatgtacg tttgacgact gacaatcaaa cagtagaaac acatcttgcc atccaaacag
     3301 gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca aaaactagca gatgctcaat
     3361 ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa cgggataaat cattgggtag
     3421 ccaaagatga ttcaagagca acgattttta cttccagtaa agatggaaca ttttctgtcc
     3481 atggattgtc ttttggaagt tacgaactag tggagattaa accgcctaaa ggatatatat
     3541 tgagtcaatc aaccattctc tttgaagttg aaaaaggcag ttatgatccg cttcatccta
     3601 taccattgga aatcatcaac gaaccaaaag caaatccgac gaataatgga aaaacgccag
     3661 ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg aggaagcttt ccaaaaacaa
     3721 gcgaagagat gcttggaggt ttctcaattt tagggttgtt gctagtcctt tcaacaggga
     3781 cagcttggtt ttataaaaaa caacaaaaag gaaacaatag gagggaaata aaatgaaaaa
     3841 catcaaaaca aaaatatgga cgtttttagg aactgctatc atgctgcttc ctttcgttct
     3901 cggcctagga actgcagagg tttctaggcc gtatcaccta caccagaaaa tgttacggtt
     3961 aatttgcata aattgaaatt cacttcagca ccagaaaatc aaataaacaa cggaacggaa
     4021 ttgacatttc cgaatagcga accgttgaac ggcgttgaat tcaacgtata tgacatcact
     4081 gctacgtact atccaagtaa agacacagca gttccagcag acgctacacc cttcgcaagt
     4141 gtaacgactt ctggtgaagg attagctaac ctgactcttc caggtaaatc tgatgggaaa
     4201 gatgctgtat acgtctttgt agaaacacca aagcccggtg tcgaaacgtc gccaaatatc
     4261 gtgttaagct taccctttta taacccgaat tcatcagacc ctgacaaacc tttagacacg
     4321 attcacctat atgctaaaaa tgtcatcaaa gaaagcaata tcaaaatcca gaaagctggg
     4381 aacttttcag gggtagataa gttagcaggg gcgcaattcc agatcaaaaa ttcagagaat
     4441 caattcgtga ccggttttga tgaaaacact ggtgtcgctc tctatgatcc tgctgagtca
     4501 gcgttgacat tcacaactga tactgaagga tccatcagtg tcaatggatt aatagacggc
     4561 aattatactt tagttgaaac aaaagcacca gatggttatc aattaaccaa tccacaaaaa
     4621 gaaactactt ttatagtaaa aaacggaaaa ttgcaacaaa caaccggtat catccatggc
     4681 gcagatgaag ccaatcttgg gaatccaaac ggtgtagtca atcgggaaaa tacaactacg
     4741 gataacacga ttactgtaga aaatgtccaa aactacggtg atttccactt catcaaacaa
     4801 gatgtgaaca cgcaagaaaa attagccgat gcagaatttt ctattctaag agcaaaagat
     4861 ccaactgtta aattaatgaa aaaaatagca aatcttgcct caggagaata cccatatgct
     4921 tggtcagatg aagtcacagg ttctgactgg gaagaagtga aattgatttc tgattcagaa
     4981 gggaaatttt caatcgaagg gctgaaacaa ggggcatact gccttcaaga aacaaaagca
     5041 ccagaaggct acgcattacc taagaatgaa gctgcgtaca ctgaatttac agtagatgat
     5101 gatccaacaa cttctgatga aacaacgatc gataaccaac ctaaaggaat cttgccttca
     5161 actggtggta tggggatcat cgtattcatc ttggtaggta ctgctttagt tggaggagca
     5221 gtgatctact tcaaaaaacg ccatcaagaa acagaagctt aaactattgg aaacaaaaat
     5281 catacgataa ataaacgata gtgattcaaa tggggagaaa ggttgtgaca agtggtcggt
     5341 ctcttggtat tcgctcaaca aacggcaaaa taggaggaac tattttgccg tttgttgagc
     5401 gaatactcag gtcaaggcgc tgatttcaca acctcttcat atacatatta gaaaataggt
     5461 ggacatgatg aataaaagca gaataagaat actggacatc ttcatggcaa tcattttagt
     5521 cgttgggatc gggatctttt cttatccttt tgtagaagat tcactgaatg attttcttgc
     5581 ccagcaaatg atcatccatt atcaaaagca agcatcgaat aagaattcag aggaaataaa
     5641 aaaacagcag gaaaaaatga caaagaaaaa ccaacagttg gcagagcaaa acgtatcacc
     5701 agggataggc agtttcaacc aagcagttga tgcaaaagca ttaaaagatt tacctagtaa
     5761 tgcctttttt atggaacata tgcttggagt catcgaaatc ccaaaaatca atgtcagctt
     5821 acctatattc gatcagacaa cggaaatatt tttgcaaaaa ggaacgtcat tattggaaga
     5881 aagttcttat ccgactggcg ggaaaaacac ccatgccgtt ctttctgggc atcgtggact
     5941 cccagaagca aaattattta cagatttgcc taagttgaaa aaaggagacc agtttttcat
     6001 ccagatcaat ggaaagacat tggcgtatca agtagaaaaa atccaagtgg tcttacctga
     6061 tgaagtagat tctttgggta tccaaaaagg cagagatctc gtgactttgc ttacctgtac
     6121 tccttatatg gtcaatacac atcgactgct tgtgacgggg catcgtattc cttatcaagc
     6181 aaaagaggca aaaaaagcga tccaaggaat tgatcaatgg aaaaaatgga aattttttat
     6241 ttggtttatc ggtatactac ttggatcaat cggcttagtt tggctactca tcgcttattt
     6301 agattctttg gccatcgcta agcgaaacta tcctttatct ttttatgtaa agaacacaaa
     6361 cggacgacca atcgaaggaa tggtcttttc tgtaaaaacg ctcaacggca aacactatat
     6421 tactagagaa aaagttccat ttgtgaaagc ttctgatgaa tacggactag tgagattttc
     6481 tgatttaaaa ggcaggaact atcgcttgca acatgaggag ctcctgctta aaatccatgt
     6541 aaaacataaa cattcgaaac agttttcaat gaaattgaaa aaaggaagat ataaactgcg
     6601 aaaagaaaaa gaggtgtact atttgattga gaaagaatga atgaagatgt ccaaaaaagc
     6661 agagggaaca gactaaatga aaaaagagag aagctgacga aatggacgtg taaactgcta
     6721 tttttggttt actatctgga acgccagctt ctattttttt atgtttcaga agaattttat
     6781 tattatagta taacttttga attagtttcc ataacatttg gagcaagggg ttaaaccacg
     6841 agcttgagct tcgcttaaag tggcaggggt atacgtacca tttccacatt tatgtgtatg
     6901 gtattttgaa ccggtcggtg tgacatagac agtttgttca ttattttgct caggagctgc
     6961 ggcttgtgcc tgctgagagg cctgttcagc tgctgcttgc tgtcgcgcct gttctgcttg
     7021 ttgctgtgcg attgcttctt gttgcgcttg ttctgcttgt tgtcgtgcaa tttcttcttg
     7081 ctttttccgt tcttcttctg cttcttttgc tttgattgtg gaatcaattg cagcaagacg
     7141 gcttgtcaac tcttgtttgt tttcaggtag cgcttggata gctgctgaag cagttgcata
     7201 gttttctttt gtaggatttg cttcagcttg tgacaaagca gattccgctt ctgtcacttt
     7261 tttttctagt gcagcttttt ccgctttttg tttctcttct tcttttttct tttcaatttg
     7321 ttcttgttct ttcgatgaat ctgccgtact ttcggagatc gtggtactgc tgctggaagc
     7381 ctgagctgaa cttgtgcttt gagtagtaga cgtagtagca ccacacccac tcaacaaact
     7441 tcctgcgacc aaaaacaatg caactaattt tttcactaaa tttcctcctt aagtaaaaaa
     7501 caaccgtttt caataaatgt ccttttcaat tgtttactta cttgttataa taattgtgtt
     7561 atgaatagtg attgaggaaa cttaatctaa tcccgttttg atacacggga ttttttgc
//
LOCUS       DACDHJ010000120         7319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDHJ010000120 DACDHJ010000000
VERSION     DACDHJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.122"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(73..693)
                     /locus_tag="IV223_001879"
     CDS             complement(73..693)
                     /locus_tag="IV223_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8712157.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(769..1206)
                     /locus_tag="IV223_001880"
     CDS             complement(769..1206)
                     /locus_tag="IV223_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8712158.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1369..2199
                     /locus_tag="IV223_001881"
     CDS             1369..2199
                     /locus_tag="IV223_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8712159.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2183..3550
                     /locus_tag="IV223_001882"
     CDS             2183..3550
                     /locus_tag="IV223_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8712160.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3569..4378
                     /gene="yidA"
                     /locus_tag="IV223_001883"
     CDS             3569..4378
                     /gene="yidA"
                     /locus_tag="IV223_001883"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8712161.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4421..4864)
                     /locus_tag="IV223_001884"
     CDS             complement(4421..4864)
                     /locus_tag="IV223_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8712162.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4998..5369
                     /locus_tag="IV223_001885"
     CDS             4998..5369
                     /locus_tag="IV223_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8712163.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5538..6320)
                     /locus_tag="IV223_001886"
     CDS             complement(5538..6320)
                     /locus_tag="IV223_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8712164.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6317..7288)
                     /locus_tag="IV223_001887"
     CDS             complement(6317..7288)
                     /locus_tag="IV223_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8712165.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         2223 a         1525 c         1266 g         2305 t
ORIGIN      
        1 aaaatcgtgt cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta
       61 agaaaataat gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc
      121 atcatcgtct tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc
      181 atcatctgtc gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc
      241 ttcatcttca tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt
      301 atcttctggg tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt
      361 gactttcttg cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt
      421 tacttcttca tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc
      481 taatgagata aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg
      541 aatttcactg tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc
      601 catgacatcg ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct
      661 tttgtttgca ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact
      721 ccttatcata caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa
      781 atccaaggtc aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat
      841 tcgaaccttc cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt
      901 agcaatctgg atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc
      961 aaatttcaaa cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac
     1021 taatttgatc gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat
     1081 gtacagcgta tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac
     1141 ttccccttgc tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa
     1201 ttccattatt cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt
     1261 gtcaaccaac ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta
     1321 gagtataata ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg
     1381 attcgactag atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat
     1441 atattaacgg aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac
     1501 tgcacagtca tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa
     1561 acactggaag atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta
     1621 tcagatgaag gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata
     1681 accatagatg acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca
     1741 ccgatacaag cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc
     1801 catggcaaaa aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg
     1861 atgtacttaa gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat
     1921 caatctgctt taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca
     1981 atggcaacac ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca
     2041 tttgcaaaag cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg
     2101 ctaaaaaaag aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt
     2161 aataacataa aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga
     2221 aaaagttttt cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga
     2281 tttgatcaat tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc
     2341 tttcactttt cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat
     2401 ttcaagacga atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg
     2461 aggctgggat gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg
     2521 tcatggtcct tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac
     2581 tgtagagatc atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga
     2641 aggttttcct gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt
     2701 ccaaatgatt tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta
     2761 tttcactgga acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc
     2821 ttatgaaggc gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt
     2881 tagccgatat caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt
     2941 aattttggag catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt
     3001 tgaactccat tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata
     3061 cctcaaactt gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga
     3121 tcctatttta aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac
     3181 tttttctggc aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt
     3241 cggatataat cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt
     3301 ttatcgccca gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac
     3361 tttgatagaa ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg
     3421 agatgagcga tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga
     3481 tttatttgac gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga
     3541 aataaattaa aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga
     3601 tggtacttta ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc
     3661 tgctgttgca aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca
     3721 ggaacagtta aacgagttga acctttttca agataatgat tacgttatta cttataacgg
     3781 ggcactcgta caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga
     3841 agatttccta gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga
     3901 tgacaaagcg atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag
     3961 tttagtcaag atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat
     4021 cgtcaaaatg atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc
     4081 gcaaactttc cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact
     4141 aaataaagaa gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga
     4201 tcaagatgaa atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc
     4261 tggtctagga gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat
     4321 cacttcatca aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata
     4381 aataaaaaaa ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga
     4441 tccatctctc gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag
     4501 ggctcaccca ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat
     4561 cagcaacaaa gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat
     4621 actcatcagc ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca
     4681 gctcacggac aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa
     4741 aaaaagcacc atttggagcc tcgattactg caatctgctg atctctttct atgacaatat
     4801 atgctgcata acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat
     4861 ccatgaatag tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct
     4921 catgaacaag cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga
     4981 aaaggagaga tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag
     5041 catcaatcga attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc
     5101 cagaaaacaa atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt
     5161 tgacttacaa ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta
     5221 tagcagctga tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta
     5281 ctgacttaaa aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg
     5341 gttataaaat cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat
     5401 aaaaaacaaa caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt
     5461 gcgtcttttt ttaacaacca ttgtagctat aaggttgcca ctctccacca atttattttt
     5521 aattcatttg aaagaaatca aataaacgag gtacctttag aaaacaaaac tgcttttcct
     5581 cctattatca cacgatcttc ttttaattca cattctagat ccccgcccct tgatgacatt
     5641 tgataagctg acaattttgt tttattcatt ctttctgccc aataaggaat aagattagaa
     5701 tgtgctgatc cacatactgg atcttcattg attgttaatt ttggaaagaa ggttctagaa
     5761 acaaagtctt tttgcacact ttctgctgtc acgatgactc ctattcctaa ttcaaaattt
     5821 ttgatagctg taaaatctgg ctgcaattta gccaccgttt tttcatcttc taatacgaaa
     5881 aataaatcac gtgctaagta ggcttcttta atcttagccc caatcgcttc ttcatattca
     5941 gacaatatcg gcactttttt cggcataata ctaggaaaat ccatgtaata ataatcttta
     6001 attcttgtca caaatagatt accgctttga gtagaaaatt taatcgtttc atcaggtatt
     6061 ttgtaatagt taaataaaac aaatgctgtt gctaatgtcg cgtgaccaca aagatcgatt
     6121 tctcgatctg gcgtaaacca tctaagctca aattcttggt ttttcttcac tgtaaatgct
     6181 gtttcagata aattgttttc aatggcaatt ttttgcatcg taccttctgg cagccattct
     6241 tctagcacat aaaccgctgc tggatttcct ttgaacacct catctgcaaa tgcatcaact
     6301 acgtaataat ctaagttcat tttaaagccc ctttctctaa tttgttataa atatattctg
     6361 cacaacaaag atcttcaata gctagaccaa ctgcgtcgaa aatcgtcggc ctcgattctc
     6421 ctttataaaa tgtttttttc tcagaaacta attcgcctaa tgtaccaaca atacaagatt
     6481 cctctaaatc tccttctgcc atggctataa gcaggtcccc gctttctgtt aaggcagctg
     6541 agtaatcatc tacatagatt tgagaagttt ccatcaacga tgtacttatc tctcttgtat
     6601 caggagtaaa tgtgcccaca gcattgatat gtgcgtcttc ccttacccac tctttttgaa
     6661 gaaatgcctc tttactagag gtcaacgtac aaatgatttc cgctggacgt actgcatctt
     6721 ctactgatat tgcatttata aacgttattt taggatactg ttttttagct ttagcgataa
     6781 aagtatttcg tctttcttct aaaatatcgt aaacaaacac tgtagtgatg ggacgaatgg
     6841 tagaaatcgc agtcaaatgc gacaatgctt gttgaccagc tccaattaaa gcaagtgttt
     6901 ctgcatgcgg atttgctaag taatctgttg ccagcgcaga tacagcagct gttcgaatcc
     6961 aggtgactgc atttgcgttt atcaatgctt tgggtagtcc gttttgagca tcaaacaaga
     7021 aaatctctcc taaatgagaa ggtaaatgat actgatgatt atccggaaaa gcggtaatga
     7081 cttttgtgcc aaaatatctg ttctccccca aataagcagg catcatcgca aacatatttt
     7141 tgccttttcc gtcaggtaaa atttccacca atcgaggatt ttggctgatt ttaccacttt
     7201 cataatcaaa aaaacatttt ttcattactt gaatcgcttc ttcaaaacta aaagaagcta
     7261 ctacttcctc aaaattaata attttcatag acaattccct cctcacaaaa gattaactt
//
LOCUS       DACDHJ010000121         7309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDHJ010000121 DACDHJ010000000
VERSION     DACDHJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.123"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IV223_001888"
     CDS             <1..376
                     /locus_tag="IV223_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8712166.1"
                     /translation="ADLFNCVPSQINYVINTRFTIQRGYLVESKRGGGGYIRIAKVRI
                     SDKKQLLEQINQLFDDTISEKNAFAIIQKLYEDQIITKKEGNLMLSAIAKNTLNFNEY
                     EDHTRARILRAFLERLSYEDGK"
     gene            383..2875
                     /locus_tag="IV223_001889"
     CDS             383..2875
                     /locus_tag="IV223_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8712167.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            3096..4019
                     /locus_tag="IV223_001890"
     CDS             3096..4019
                     /locus_tag="IV223_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8712168.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            4037..5278
                     /locus_tag="IV223_001891"
     CDS             4037..5278
                     /locus_tag="IV223_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8712169.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            5412..6758
                     /gene="gor"
                     /locus_tag="IV223_001892"
     CDS             5412..6758
                     /gene="gor"
                     /locus_tag="IV223_001892"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8712170.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         2530 a         1184 c         1600 g         1995 t
ORIGIN      
        1 ggcagacctc tttaactgtg ttccttcaca aatcaactat gtgattaata cccgttttac
       61 catccaacgt ggttatctag tagaaagcaa acgtggtggt ggtggttata tacgaattgc
      121 aaaagtaagg atttcagata aaaaacaatt acttgagcag ataaatcagt tatttgatga
      181 tacaatcagt gagaaaaatg cttttgctat tattcaaaaa ctttatgaag accaaataat
      241 tacaaaaaaa gaaggaaact tgatgttgag tgcaattgca aaaaacacat tgaatttcaa
      301 tgaatatgaa gatcatacac gtgctcggat tctccgagca tttctggaac gtttaagtta
      361 tgaagatggg aagtgagatt agatggaaga actttttact gaaagagccc aagcagtatt
      421 agaaattgca caagaagaag caaaacggtt gaaacatcaa tcagttagtt cagaacatgt
      481 tttactagcc ttagtggtcg agcaaaatgg aattgctgga aaagtattac gtgaaatgga
      541 tttgaatgaa acggaaattt atgaagaaat tgaacatctg attggttatg gtggtatccg
      601 ttcttaccct aaaggagtct ttcttccata ttcaccaaga atgaaacaag tttttgcttt
      661 agctagcagc gaagtgaaga aaactggttc tcaaaaagtt ggaacagaac atattttgat
      721 gagcttatta aaagatgaaa gtatcatggc tactcgaatc atgatcaact taggtatcag
      781 cctttcaaaa gcccgtcaag ttctgaaaca aaaaatgggg ctagctggtg attctgctgg
      841 aaaaggaatg aatcgtcgta gaaatgtaaa tgtacaagat aaacggcaaa caccgcaagg
      901 aacacctacg ctagattcat tagcacgtga tttgacgaaa ttagcaaaag aaaacaagct
      961 ggacccagtt gttggtcgtt cgagagaagt gaaacggctt atccagattc ttagccgacg
     1021 gacaaagaat aaccctgtac tggtgggtga acctggtgta ggcaaaacag ccattgctga
     1081 agggttagct caaaaaatca ttttaggtga agtgccagaa gacatgcaag aaaagcgcct
     1141 gatgatgctt gatatgggat ctttggttgc tggtacgaaa tatcgcggtg aatttgaaga
     1201 tcgaatgaaa aaggtcatcg acgaaatcta taatgatggt caagtaatct tattcattga
     1261 tgaattgcat accttaattg gtgcaggtgg agcagaagga gcaattgatg cttcgaatat
     1321 cttgaaaccg gcgttagctc gaggagaatt acaaacgatc ggagcaacga ccctagatga
     1381 atatcaaaaa tatatcgaaa aagattctgc attagagcga cgttttgctc gcattcaagt
     1441 agatgagcca actcctgaag aagcagaagt gattcttcaa ggattacgta caagatatga
     1501 agagcaccat ggagtggaga tcacagatga agctgtacga gcagcagtca atctttctgt
     1561 tcgttatatc acttctcgtc aactacccga taaagcaatt gatttgatcg atgaatcagc
     1621 agcgaaagtc cgtttggatc aaaccgatca cttaacaaaa tcaactgtga tcaaactaga
     1681 aatcgatgaa ctagtacaag aaaaggaagc agcgatccaa aaacaagact ttgaaaatgc
     1741 tgctcaatta cgcagacaag aaaaagcact acgtaaaaag ctgcaaaaag tttctgcaat
     1801 tgaagcaaaa caggagcaag gttattctga tcgtgtaaca gaagaagatg ttgctacagt
     1861 tgtatcggag tggactggtg tccctctaca gcagttggag aaaaaagaaa gcgaacgctt
     1921 gttagaactg gaaggacttc tgcatgaacg tgtcgttggt caagatgagg ctgtcaaagc
     1981 agtttcacgt gcgatccgcc gtgcacgaag cggattgaaa gatcctgatc gtccaattgg
     2041 ttcattcatg ttccttggac caactggtgt cggaaaaaca gaattggcca aagcactttc
     2101 agaagtcatg tttggaagtg aagatgccct gatccgtgtt gatatgtcag aatttatgga
     2161 aaaatacagt acaagccgat taatcgggtc tcctccagga tacgtagggt atgatgaagg
     2221 tggacagttg acagaaaaaa ttcgtcaaaa accatattca gtgattctct tagatgaagt
     2281 ggagaaagca catccagacg ttttcaattt acttctgcaa gtattggatg atggtcattt
     2341 aacagattct aaaggtagaa aagtggactt tagaaataca atcatgatta tgacttcaaa
     2401 tatcggagcg acacaaattc gagaagaaaa aaatgtcgga ttcaatgtcc aagatgttac
     2461 taaagatcac aaagccatgc aaaaacgtat cttagaagag ttaaagaaag ctttccgacc
     2521 tgaattcttg aatcgaatcg atgagactgt cgtcttccat tcattgagta aagatgaaat
     2581 ccatacaatc gtccaaatca tgagcaaagc gattatcaaa cgattgaaag aacaagatat
     2641 tcaagtgaaa atcacaccat ctgcaatcga tgtgatcggg aaagctggat ttgatcctga
     2701 atatggtgca cgtccaatcc gtcgcgcact tcaaaaagaa attgaagatc gattgagcga
     2761 ggctttgctt ggcggagaaa ttcgtttagg tgaccacgta acagtaggtg cttcaaaagg
     2821 taagatcaca ttgaatgttc gcggacctaa aaaggaaaca gtaggttccc tatagttact
     2881 tggcctgtgt agctgaattt ttataatagt aagacttgat taagaggttg tgacaagatt
     2941 tttgtcacaa tctcttttta tatttggaca gaatatatcg aactttattt tgattttttg
     3001 tgaaataatt ttcaaaaatc cgactttaaa tagaagaagt attggaaagt ttttagagct
     3061 atgctactat tatgaagttg taggaggaga aaattatgat cgaactcata accacagtag
     3121 aaactattga acaagcagaa cagttgcttg ctgcaggtgc agatacacta tatttcggtg
     3181 aagagcaatt tggattgcgt ctgcctgctt catttacacg agaagaacag cgacaattgg
     3241 tagaattagc acacaagtat ggtaaaaaag cgaatattgc tgtaaatggg atcattcatc
     3301 ctgaaaaaat gaaaaatgta cctgaatatt tggctttttt aaaagaaata aacgtagatg
     3361 tgatcacagt tggtgataca ggtattattt atacgatgaa aaaaaatcct gaattgcgta
     3421 taccgtatat ttatgatgct gaaacattag taacgagcgc acgtcaaatc aatttctggg
     3481 cgaaaaaagg cgcagttgga gcagtacttg ctcgtgaagt tccttttgaa gagatgaagg
     3541 cgatggaaga aaaactcgat attcctgtag aaacccttgt gtatggtgct acatgcatcc
     3601 atcaatccaa acgtccactg ctacagaatt actataacta tacgaaacaa gacgaacaaa
     3661 aagatcgtga aagaggtctt tttatctcgg aacctaaaaa agaagagaca cattattcta
     3721 tttatgaaga tagtcatggc acacatattt ttgccagcaa tgatctgaat ctctcgaatg
     3781 aattgaatga actttatcag catcattact gtacatggaa attggacgga ttatttactc
     3841 caggcgaaaa attcgtaaac atcactcgac tattcgccga agcaaaaaaa caaattttag
     3901 aaggtagctg ggatcaagaa aaagcggaaa aaatgactag cgaaatcagt aaattgcatc
     3961 ctgaaaaccg tgaattagat acaggattct tttatattga tccttctgct atcaaataag
     4021 gagtatgatg aaagaaatga cagtaaaaag aaaattgaaa cgaccagaaa tcctagcgcc
     4081 agcaggaaca ctggaaaagt taaaaaccgc gatccattat ggagcagatg cagtatatat
     4141 tggcgggaat gcatatggtt taagaagccg tgcaggtaat tttacttatg aagaaatggc
     4201 tgaaggagtt gcttttgcaa aagaacatga tgcgaaggta tacgttgctg ctaatatggt
     4261 cacccatgaa ggaaatgaag atggagcagg ggaatttttc cgcagtttgc gtgatgtcgg
     4321 tatttctgca gtgattgttt ctgatccagc tttgatcgaa atttgtgcga cagaagcccc
     4381 aggtctgccg atccatttat ccactcaagc ttcagcaact aattttgaga cattggaatt
     4441 ctggaaagaa gaagggttgg aacgtgtcgt tttagctcgt gaagtttcaa tggaggaagt
     4501 ggcagagatt cgtaaaaata cggataccga aatcgaagca tttatccatg gtgccatgtg
     4561 tatctcttat tctggtcgat gcacattgtc caaccatatg tcgatgcggg atgcaaaccg
     4621 tggcggatgt tctcaatctt gtcgctggaa gtatggattg ttcgatatgc cttttggtca
     4681 agaacgaaac agtttaactg atacaggcga agtggaagaa gaattctcga tgagtgcagt
     4741 ggatatgtca atgatcgaac atattccaga attgattgaa aacggcgtag acagcttcaa
     4801 gatcgaagga agaatgaaat cgatccatta tgtatctacg gtgtcaaatg tctataaagc
     4861 ggctgtagac agttatatgg aagatcctga aaactatgtc tgcaagcaag aatggatcga
     4921 cgagttatgg aaagtagcac aacgagaatt agctacaggt ttttactaca atacaccaac
     4981 tgaaaacgaa caattattcg gtgaacgtcg taaaatccca gtatataaat ttgtcggtga
     5041 agtattatct tatgatgagg caacacagat tgctacgata cgtcagagaa acttgttttc
     5101 tgtaggagat gaaatcgaat tttatggtcc aggattcagt catttcagtc aaaccgttga
     5161 agttatgcat aatgaagaag gcgaatccat tgaccgagca ccaaatccaa tgatgatttt
     5221 aactatgcca gtcgcacagc cggtgaaacc tggagatatg atccgaaaac gtaaataagg
     5281 aaaaagctga tatatccctg ttttaagcat cttcgcatac aaaattcaag aaaagtgtta
     5341 cacttgtttt ctgtagaaaa aggatcgtta aataaaggtt ttgattgatc tcttcaataa
     5401 ggaggaacaa aatgcaaata tatgattaca tcgtaattgg tggcggctct ggcggtattg
     5461 cttcagcaaa cagagcaggg atgcatggcg caaaagtttt gctgattgaa gcgaatgaat
     5521 tgggcggaac atgtgtcaac gtcggttgtg tacctaaaaa agttatgtgg caagcaagta
     5581 cgattttgga aacaattgaa cgagatgcct caagttacgg tatccaagca gatttaaaaa
     5641 atgttgattt cgccggtcta gtagaaagac gagaaaaata tattgatttt ctacacggtg
     5701 cttatcaaag aggattagat agcaatcatg tcgaggctat aaaaggttac gctactttta
     5761 tcgatagtca aacgatcgaa gtaaacggtg aaacctatcg tgctcctaaa atcttgatcg
     5821 ctactggagg ccgtgcaagt aaaatgacca ttcctggtgg agaatacgca attgattcaa
     5881 atggattttt tgaattaaca gagttaccta agagagcaat cgttttaggt gctggataca
     5941 ttgcggctga actttcaggt gtatttcgtg gtttaggtag tgaagtcatg tgggcatatc
     6001 gaaaagaacg accacttaga acgtttgata aaatgctttc ggacaatttg atccaaatgt
     6061 accaagaagc tggaataaaa acttacgcac atcatattgc taaagaaatt actaaaaaca
     6121 atgacgaata tacggtgcta tttgaaaatg gtgaaacctt aactggtgat tgtgtacttt
     6181 ttgccggtgg acgtgtaccg aataccgaaa aattaggtct ggaaaataca aatgtggaat
     6241 tggacaaaaa agggtttatc aaagtagata aataccaaaa tacaacggat gaacatattt
     6301 ttgctgttgg tgatgtgatc ggtaaattag atctgactcc tgttgcaatc gcagctggta
     6361 gacgtttatc agaacgttta tttaatggaa agaaagattc ttatcttgac tataaacttg
     6421 ttccgacagt cgtctttacg catccaccaa ttgcaacaat tggtttgaca gaagaagaag
     6481 cattggaaaa atatggcgaa aatgaattga aagtttatcg ttcacgcttt accccaatgt
     6541 actttgcatt aaatgattat cgccaaaaat gcgaaatgaa attgatctgt gtcggaaaag
     6601 aagaaagaat cgtcggctta catgctattg gtgttggtgt cgatgagatg ctgcaaggtt
     6661 ttgccgtggc tatcaagatg ggtgcaacta aagaagactt tgacaataca gtagccatcc
     6721 atccaacagg agcggaagaa ttcgtaacga tgcgataatt taatattaac agttccaaca
     6781 gctttttatt gaacaaacca tgtaaaacca gaaaatcagt atactgattt tctggttttt
     6841 tgttttttag ttatttatgt aagattgctt cgataattct caaaacgaaa taagtaggga
     6901 agttggagat tttgattatc ttcgtaactg acgaagagta aatgaatcca tttggtatgc
     6961 actcttaagt tcaaaatatt ggtatcgatg tttaaaagaa atagaaatgt atcgaagaac
     7021 agatgtttaa aaaagtgtga ctttgtataa ataaattaga agaagcaaac gaggatgtta
     7081 aaagaatacc atatagttac caaaccaaga aagttctcgt caagttcgct ggtacgaatc
     7141 aaatcaagtg aaaaaaggga ctgtgaaaaa aattgtgtca tagttagtca ctttttccga
     7201 acaaatagtg ttcaatcagc tgtccttcgg tattagctca aaaaacggca aaatatgagg
     7261 aactattttt ccatttgttt ctctaatact caggacaaaa cactgattt
//
LOCUS       DACDHJ010000122         7223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDHJ010000122 DACDHJ010000000
VERSION     DACDHJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.124"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV223_001893"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IV223_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8712171.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="IV223_001894"
     CDS             complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="IV223_001894"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8712172.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2466..4544)
                     /gene="topA"
                     /locus_tag="IV223_001895"
     CDS             complement(2466..4544)
                     /gene="topA"
                     /locus_tag="IV223_001895"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8712173.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="IV223_001896"
     CDS             complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="IV223_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8712174.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5579..6346)
                     /locus_tag="IV223_001897"
     CDS             complement(5579..6346)
                     /locus_tag="IV223_001897"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8712175.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="IV223_001898"
     CDS             complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="IV223_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8712176.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         1864 a         1606 c         1274 g         2479 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgccagaaaa
     1081 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     1141 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     1201 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     1261 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     1321 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     1381 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     1441 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     1501 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     1561 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     1621 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     1681 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     1741 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     1801 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     1861 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     1921 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     1981 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     2041 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     2101 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     2161 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     2221 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     2281 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     2341 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     2401 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     2461 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     2521 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     2581 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     2641 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     2701 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     2761 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     2821 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     2881 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     2941 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
     3001 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     3061 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     3121 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     3181 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     3241 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     3301 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     3361 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     3421 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     3481 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     3541 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     3601 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     3661 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     3721 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     3781 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     3841 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     3901 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     3961 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     4021 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     4081 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     4141 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     4201 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     4261 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     4321 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     4381 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     4441 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     4501 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     4561 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     4621 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     4681 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     4741 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     4801 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     4861 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     4921 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     4981 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     5041 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     5101 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     5161 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     5221 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     5281 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     5341 ttcctgccaa cttttgcgaa acagctcccg atgtttggtt attttaccta tcttaatgat
     5401 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     5461 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     5521 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     5581 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     5641 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     5701 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     5761 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     5821 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     5881 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     5941 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     6001 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     6061 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     6121 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
     6181 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
     6241 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
     6301 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
     6361 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
     6421 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
     6481 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
     6541 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
     6601 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
     6661 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
     6721 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
     6781 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
     6841 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
     6901 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
     6961 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
     7021 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
     7081 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
     7141 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
     7201 gttctctcca ttcctttcat acc
//
LOCUS       DACDHJ010000123         6992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDHJ010000123 DACDHJ010000000
VERSION     DACDHJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.125"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(251..838)
                     /locus_tag="IV223_001899"
     CDS             complement(251..838)
                     /locus_tag="IV223_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712177.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1013..2335
                     /locus_tag="IV223_001900"
     CDS             1013..2335
                     /locus_tag="IV223_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8712178.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2490..3266
                     /locus_tag="IV223_001901"
     CDS             2490..3266
                     /locus_tag="IV223_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8712179.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3259..3828
                     /gene="rnmV"
                     /locus_tag="IV223_001902"
     CDS             3259..3828
                     /gene="rnmV"
                     /locus_tag="IV223_001902"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8712180.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3821..4705
                     /gene="rsmA"
                     /locus_tag="IV223_001903"
     CDS             3821..4705
                     /gene="rsmA"
                     /locus_tag="IV223_001903"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8712181.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5156..5785
                     /locus_tag="IV223_001904"
     CDS             5156..5785
                     /locus_tag="IV223_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712182.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5909..>6992)
                     /gene="ugpC"
                     /locus_tag="IV223_001905"
     CDS             complement(5909..>6992)
                     /gene="ugpC"
                     /locus_tag="IV223_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8712183.1"
                     /translation="YKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGKSTTLRMIAG
                     LEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGLKLRKYDKAD
                     IKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMDEPLSNLDAK
                     LRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIGTPKEVYDTP
                     VNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGYEGKEVIFGI
                     RPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKVDARDFHKPG
                     EIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         2205 a         1242 c         1447 g         2098 t
ORIGIN      
        1 gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct tacattcata
       61 aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag cgttgatgtg
      121 ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat gtgtgattga
      181 aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc tttattttgt
      241 aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa cggcttggta
      301 agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc gtacgtccga
      361 taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca
      421 aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt gccatcactc
      481 caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc tttgtaaatg
      541 ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc
      601 caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg atcaatgcaa
      661 cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca acagtcatta
      721 atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct ttacgggaaa
      781 aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta aaatccataa
      841 actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa agaaatacat
      901 ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa attaaaatat
      961 tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg
     1021 caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg gaaccgtgat
     1081 cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt ctacaagttt
     1141 gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat tgacagtggc
     1201 tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg aagctgcgta
     1261 tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact ttcctgctaa
     1321 tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac aattatctac
     1381 cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt tgaatttgtt
     1441 agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat taataccaat
     1501 tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc agttatttcc
     1561 aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt tgttagcaac
     1621 gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc
     1681 ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag tcgtttatct
     1741 attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg gtaatttgaa
     1801 tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc tggtcactat
     1861 tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg ggatccgcgt
     1921 gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga caaagctttc
     1981 aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag cttgtattat
     2041 gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga tgtggctatt
     2101 cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg aattgccacg
     2161 tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg gtgctatttt
     2221 cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta taggtgttac
     2281 cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat
     2341 ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc tttttctatt
     2401 tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt gcttatgtgc
     2461 taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc catttaaatg
     2521 ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa atgggggtaa
     2581 cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg caattaagcc
     2641 atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct ggcagttata
     2701 cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt gtagcattag
     2761 gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa gataaagtat
     2821 ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt catactagag
     2881 aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat atcggtggaa
     2941 tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac ttagggatgc
     3001 atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa gaggctgcag
     3061 caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg gcaccagttc
     3121 cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa aagattgctg
     3181 aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat
     3241 tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt cgttgaagga
     3301 aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga aacaataggg
     3361 tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac tcgtggagtg
     3421 atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat tatggaagct
     3481 gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga
     3541 agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa aaaggtcgtg
     3601 acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct tttagactat
     3661 ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc
     3721 atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg tattacgccg
     3781 aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat
     3841 tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat ttaaaaaaag
     3901 tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca
     3961 aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat tgacggaaca
     4021 attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt tgatccctgt
     4081 attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag atattttaaa
     4141 gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt taaaagtcgt
     4201 agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg aatcagattt
     4261 ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta tctctgctga
     4321 accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata tggaagccag
     4381 tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag attcagcgat
     4441 tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa aagcattttt
     4501 ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata acttgcagca
     4561 ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg aaacagctgg
     4621 aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg cattaagcaa
     4681 tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg
     4741 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt
     4801 tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg ttgtgggcct
     4861 gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat gtttttgagt
     4921 tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac aaggagatgt
     4981 gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc ttatacttga
     5041 tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag ttattagtaa
     5101 gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag aaaaaatgag
     5161 aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg gggtcattat
     5221 cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg cgtctcatgt
     5281 gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg cgctaggtac
     5341 gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat tgtcacatct
     5401 tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa
     5461 tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga tcggtgtgat
     5521 tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta atatgcctga
     5581 aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga ttggtgggct
     5641 tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac tcagcaaata
     5701 ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg
     5761 gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc tcatctagta
     5821 atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg caccaacatc
     5881 ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt tctgtttctt
     5941 tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg cctggtttat
     6001 ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt gtgtaaagca
     6061 tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg gcttcaggtg
     6121 aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg aagataactt
     6181 cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga atacgaagct
     6241 taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag aagttcatgg
     6301 caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa acttctttcg
     6361 gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca gccatagtca
     6421 ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt
     6481 tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat aatggctcat
     6541 ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt
     6601 gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct aagatctctg
     6661 ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat ttcaagccaa
     6721 aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg aaaaccatgg
     6781 caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata tttagttctc
     6841 cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg
     6901 aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa tcagttacag
     6961 aataattttc tgcattgtca tattttttat at
//
LOCUS       DACDHJ010000124         6957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDHJ010000124 DACDHJ010000000
VERSION     DACDHJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.126"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..193
                     /locus_tag="IV223_001906"
     CDS             <1..193
                     /locus_tag="IV223_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8712184.1"
                     /translation="FRLTKATFLDCLKTLIYSISLPTIIATILMVFLPSFDTSAFIAF
                     AGIFIFAQAVKGWAKISIN"
     gene            371..1318
                     /locus_tag="IV223_001907"
     CDS             371..1318
                     /locus_tag="IV223_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8712185.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1386..1679)
                     /locus_tag="IV223_001908"
     CDS             complement(1386..1679)
                     /locus_tag="IV223_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8712186.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(1707..2372)
                     /locus_tag="IV223_001909"
     CDS             complement(1707..2372)
                     /locus_tag="IV223_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8712187.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(2512..3144)
                     /locus_tag="IV223_001910"
     CDS             complement(2512..3144)
                     /locus_tag="IV223_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8712188.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(3151..4218)
                     /locus_tag="IV223_001911"
     CDS             complement(3151..4218)
                     /locus_tag="IV223_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8712189.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(4215..4946)
                     /locus_tag="IV223_001912"
     CDS             complement(4215..4946)
                     /locus_tag="IV223_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8712190.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(5115..5594)
                     /gene="greA"
                     /locus_tag="IV223_001913"
     CDS             complement(5115..5594)
                     /gene="greA"
                     /locus_tag="IV223_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8712191.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(5730..6905)
                     /gene="mltG"
                     /locus_tag="IV223_001914"
     CDS             complement(5730..6905)
                     /gene="mltG"
                     /locus_tag="IV223_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8712192.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2025 a         1454 c         1087 g         2391 t
ORIGIN      
        1 ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc
       61 attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc
      121 cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat
      181 atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac ccttttagag
      241 gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa
      301 acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg
      361 gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat
      421 cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac aaccagagga
      481 tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc
      541 ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga
      601 gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat
      661 tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa
      721 ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa
      781 aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc
      841 attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa
      901 gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat
      961 cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga
     1021 tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt
     1081 gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc
     1141 tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc
     1201 aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag
     1261 ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata
     1321 gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc
     1381 tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca
     1441 agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca
     1501 tccatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa
     1561 ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct
     1621 ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc
     1681 ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca
     1741 tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca
     1801 ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt
     1861 tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt
     1921 tctgctaaag aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa
     1981 ttatgcgcaa tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc
     2041 ttttcaagta ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc
     2101 atttccttta ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca
     2161 attacatgat cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt
     2221 gccacaacag catgagtggc tatattcata tattcattga cccgatcttc attactatca
     2281 atagccagta cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt
     2341 ccaagaccta caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc
     2401 tcagtataac aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag
     2461 aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac
     2521 caaaccatgt ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc
     2581 caatatattg gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat
     2641 ttcctgatta ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag
     2701 ttcttcatgt aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac
     2761 ttctggttct aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg
     2821 tgcggtagag gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt
     2881 ttcgtcatca ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat
     2941 tgcttttgtt gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc
     3001 cggtcgaagt gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac
     3061 ttcaatgtct tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc
     3121 atctactaat aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat
     3181 ttctacactt gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc
     3241 tactcgttct ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa
     3301 ccctgttccg tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac
     3361 ctctaattca ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg
     3421 gaacaagtga tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga
     3481 aatctggatt ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt
     3541 ctcttctaaa ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc
     3601 gttgatgatc tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt
     3661 ttctgcttgc tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac
     3721 tgaatcatgc agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt
     3781 ttgtccatct acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat
     3841 cattttttct tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga
     3901 tgcacttggg atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg
     3961 cagcttttct tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac
     4021 aattaatcct acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa
     4081 acgtgcacgc agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa
     4141 aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct
     4201 ggatattttt cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata
     4261 atcttcaatc tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt
     4321 ttcaaagaaa attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat
     4381 cggatggcta cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc
     4441 acaatattat catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa
     4501 atgacggcga tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg
     4561 aataattgct gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc
     4621 atgatctgtt tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca
     4681 cctttcagac caataaataa aacggcaaat acagccatca tccataaagc aggactgttg
     4741 actaagctaa aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta
     4801 cgaggatgtt tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa
     4861 agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc
     4921 acaaaaaaac gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca
     4981 tcatacctat ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa
     5041 tctaaaatac acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag
     5101 atctttcagt cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct
     5161 ggagtagcaa tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga
     5221 gaatcattgg aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac
     5281 tcttcttctt ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca
     5341 tctgcatcta caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt
     5401 gtgattcttc cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg
     5461 gaaagatctc cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact
     5521 gtttttagtt cttctaattc tttttctaat ttttctttac cttctagagt cattggataa
     5581 actttctcta ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt
     5641 ttgaggaata tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta
     5701 aaaatgatca atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat
     5761 tccatatgtt cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg
     5821 aaataataat agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga
     5881 ttgtcaaaag gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca
     5941 gctgtatctt catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata
     6001 tctgactgaa gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta
     6061 cgatcatttt cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt
     6121 acagtcaaat ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg
     6181 ttcaccattt caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct
     6241 gggaataagt aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac
     6301 agttctggga atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa
     6361 aattctttct tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg
     6421 tacccttctg gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct
     6481 tcttgcaact gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca
     6541 gcttgaaagc cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta
     6601 ataatattgt ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc
     6661 tcgacttgga ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa
     6721 cgatacacgg taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa
     6781 atgatcttcc tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata
     6841 tctgctgtag ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct
     6901 gacaaaacaa ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatat
//
LOCUS       DACDHJ010000125         6932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDHJ010000125 DACDHJ010000000
VERSION     DACDHJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.127"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..590
                     /locus_tag="IV223_001915"
     CDS             117..590
                     /locus_tag="IV223_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8712193.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            587..2719
                     /gene="feoB"
                     /locus_tag="IV223_001916"
     CDS             587..2719
                     /gene="feoB"
                     /locus_tag="IV223_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8712194.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2721..2855
                     /locus_tag="IV223_001917"
     CDS             2721..2855
                     /locus_tag="IV223_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8712195.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2949..3593
                     /locus_tag="IV223_001918"
     CDS             2949..3593
                     /locus_tag="IV223_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8712196.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3780..4973
                     /locus_tag="IV223_001919"
     CDS             3780..4973
                     /locus_tag="IV223_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8712197.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4966..6693
                     /locus_tag="IV223_001920"
     CDS             4966..6693
                     /locus_tag="IV223_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8712198.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2230 a         1222 c         1557 g         1923 t
ORIGIN      
        1 taccgagagt attctatgtg agaatgattc tcatttattt gcgtaacacg taagaaaatt
       61 agaacatata atgaatgatt ttaatacatg atttatttca caagagagga atcaatatgc
      121 agaccttagc gcaggcaaag cagaatttcg tttatcaagt cgaacgaatc gaaacagaag
      181 aaaaaacaaa acgccatttg caaaatatcg ggatcgttgc cggaagtaaa atcgtcgtcg
      241 tcaatttatc aggcaacaat gccattttac tggtaaaaag tcatcgagtg gcagtaagtc
      301 gggaaatact aggtcaaatc tttgtcaata aaaagactgc ttcctctgaa agctggactt
      361 ctcttgatca attaaaagca ggagagactg ggatcgtcgt agcaatccac ggacaaggcg
      421 cagtgaagcg acggttaatg gatatgggat tgacgcgaaa tgtaaaaata gttatccgaa
      481 aactagcccc tctgggagat ccaatcgaac tgacagtgcg agggtatgaa ctgacgttac
      541 gaaagtcaga ggccgaacta atcttgatac aaagggagga aaaagaatga atatcgcgtt
      601 agtcggaaat ccaaacagcg ggaaaactag cttgttcaat ctcttgactg gttcgaatca
      661 atatgtaggg aattggccgg gcgtaacagt agaaaaaaaa gaaggatcgt tgaaaaaaaa
      721 tccgaacatt catattcagg acctacccgg catctattct ttgtctcctt atacaccaga
      781 agaagtgatt gcaagagatt atttggttca ggaacagcct gaactgatca tcaacattat
      841 tgatgctacc aatttagaac gaaatcttta tttaactacg cagttattgg atttagacat
      901 tcctatgatc attggtctga atatgatgga tgtcatcaaa aaagaaggaa aaaaaatcaa
      961 tctagaaaag ctttcctatg gtcttggtgt accagtttta ccaatcagcg tattgaaaaa
     1021 gcaaggaatc gaaaaactaa tcgaaaagat tcaggagtct gcaaaaaagc ggccttctat
     1081 tcctgttccc tgtacatatg atccacgttt agaagcagct ttacatgaaa ttgaagatgt
     1141 actaggagaa caaagcggca agaaacgctg gtatgcaatg aaatattttg aacgagatca
     1201 gaaggtaaga gaggattacg agatagcagt ttctcagcaa aaagagatcg agcaactgat
     1261 ccagttaaca gagaaactat tggacgacga tagtgagacg attctggtca atgaacgtta
     1321 tgaatttatt acccaactat gtacactctg tgttgtgtca aatgattctt tccaattatc
     1381 gatgagtgac aaaatcgatc agattgcaac gaatcgttgg ttagccttgc cgatcttcgc
     1441 atttgtgatg tggctgatct attatctggc tattcaaaca gtaggaacaa tggggacaga
     1501 ttggatcaat gacacattgt ttggtacatg gttaccagaa catgttggca gattgttagc
     1561 tgaatggcaa gtagcgggat ggatgcagga gctgatcttg aacggcatca tcgcaggcgt
     1621 gggagcagtg ttaggatttc taccgcaatt gattatattg tttttatgct tgagctttct
     1681 ggaagactgc ggatatatgg cgaggatcgc ttttgtcatg gatcgacttt tcaggaaatt
     1741 cggtctttca ggaaaatcct tcattcctat gctgattgct acaggatgtg gagtgccagg
     1801 cgtcatggcc agtcgcacga ttgaaaacga gcaagatcgt cgtttgacga tcatggtcac
     1861 aaccttcatg ccttgttctg caaagttgcc gattattgcc ttagtagcag gggccttttt
     1921 tcctcatcaa tcttgggtag ctccttctgc ctattttctt ggtatgactg caattatttt
     1981 ctctggaatc agcttgaaaa aaacacgttt gtttgctgga gatacagctc cttttatcat
     2041 ggaattgccc gcatatcatt ttcctcaatg gtcagcggtc ctaagacaaa cctatgaccg
     2101 cagcaagtct tttgttaaaa aagcaggaac gattatcttt gtttctagta tcgtgatctg
     2161 gttcagctct tcttataatt tttatgctca acctgtttct gaagaccaaa gtatcttagc
     2221 ctttttcggg cgaatcctgg cagttgtttt tcagccgctt ggttggggaa actggcaagg
     2281 aacagtcgca gcgattacag gattggtcgc taaagaaaat attattggca catttggtat
     2341 tttgtttggt catgcggaag tatccgaaaa tggcagagaa atctggcaag tgctacaaca
     2401 cacatatact cccgcagcag cttattcatt gttagccttc aatttacttt gtgcgccttg
     2461 ttttgcggca attggggcta tccatcggga aatgaatgcc aagaaatgga cttggatcgc
     2521 cataggctat caatgcggtt tagcttattt agttagtttt gtgatttatc agttgggaca
     2581 cttattggag ggtggacagg tagcgttagg gacgtatctt gctattttac tgatgatagg
     2641 actcgtttat gttttgttcc gtcagccaaa atccaagaac cagttttata ctatattgtc
     2701 attagaagga gaggaatagg atggcgacat tcttattaag tgtgttaatc tttggaagtg
     2761 caggcgcaat tgtctatact cgtttaaaat ccggtaagaa ttgtgatgac tgtcaaacag
     2821 cttgcccagt aaaaaaggaa cagatcaagc gatgatcagt cgttgcaaga ggctattctc
     2881 cccttttttg cttattttgc tagagcatag taagatgagg gaaagtaaaa aaagaaagag
     2941 gaaagaaaat gtctggaaag aaaaaaatcg tccgcccgcg ctaccagcag attgctgttg
     3001 atttagccga acgtatcgtt gaaaaccgat ataaagtggg tgagaagatc catgcccgct
     3061 caacacttgc cagtacgtac aatgcttctc ctgagactac ccgcaaggct atcaatgtgc
     3121 tggtagatct tggaatcatg gaagtacggc atggaagtgg ggcatttgtt gcttcgaagg
     3181 aaaaagcaaa agatttccta ttgcaatatc aagatgtaca atccatccaa gatacaaaag
     3241 cagaaatcat ggcaagtgtg gagaagcaac aagatgagct gaatcatttt tctcaattat
     3301 tggatcagct ggtcaatcaa acgaagcgaa tacataagat gaatccttta cttccgtttg
     3361 aattatcttt atcagaaaaa gcaatgcatc tggaccgttc aattagcgag ttgaatatct
     3421 ggcaggcgac tggcgccaca gttatcgcaa tttctcataa tgaagaactg cttgtttcac
     3481 ctggccccta tgctaaattt acggcagggg acatcgttca ttttattgga aatgaatata
     3541 cactacagcg tatgacaaac tttttttacc cggagcatca gcttgcagaa tgattgtgtg
     3601 aaatattaaa aaaatattaa cttggctaaa ccttttattt acaaagattt agccaagttt
     3661 ttttcagttt ggacaagcaa atttctttca tttttgacaa agtgactaca tgatgttaac
     3721 atagtgtggt cacttttttc agaaagaaaa atgaatttac ataaaaaagg agaatttgtt
     3781 tggtaaaagt agaagtaaaa catttaacaa agatatttgg taaaaagaca caagccgctt
     3841 tagatatgat gaatgatcac cagccgaaaa cggagatttt aaagaaaacc ggtgcaacgg
     3901 ttggtgtgta tgacgtgaat tttgacgtaa aagaaggaga aatcttcgtg atcatggggc
     3961 tttccgggag tgggaaatcc acattgatcc gtctattgaa tcgtctgatt gagccaactt
     4021 cgggcagtat ctatattgat ggagaagatg tatcgaagtt aagcaaagaa gaattgcgag
     4081 aagtccgtcg tcataaaatc aatatggttt tccaaaactt cggtctgttt ccacatcgaa
     4141 cgatcttgga aaatacagaa tacgggttag aagtccgtgg tgtacctaaa gaagaacgac
     4201 aagaaaaagc agaaaaagca ttagaaaatt ctagtttgct ttcatttaaa gaccaatatc
     4261 ctagtcagtt atctggtggg atgcaacaac gggttggcct agccagagct ttagcaaatg
     4321 atcctgagat cttgctgatg gacgaagcgt tctcggctct tgatccgctg atccggcgag
     4381 aaatgcaaga cgaattactg gacctgcaag ccaatgttca aaaaacgatt attttcatta
     4441 cccatgattt gaatgaagct ttacggattg gggacagaat cgccttgatg aaagacggcg
     4501 aaatcatgca gatcggaaca ggtgaagaaa tattgaccaa tccagcaaat gattatgtac
     4561 gtgaatttgt ggaagaagtc gatcgttcca aagtgcttac ggctcaaaac atcatggtac
     4621 ctgcattgac gacgaatatc gaatctgatg gaccaaacgt tgcattgaca aggatgcgta
     4681 atgaagaagt cagcatgctg atggctgttg acagaaagag acacctgaaa gggatcatca
     4741 ctgcagatca agcgttagaa gctaggaagc aaaaacgtcc attgatcgac ttcctagatg
     4801 aaaatgtgac ggtgattggg aaagacatgg tggtaagtga tattttcaat attatttatg
     4861 actcaccgac tccacttgca gttgtagagg atggaaaatt aaaaggtgtc gtcatccgag
     4921 gtagtgtgat tgaagcatta gcagaaacaa gcgaggtgag tgaacatgaa taatctattg
     4981 aactatcaat taccagtggc aaactgggta gaaaatatca cagaatggtt tacgacaact
     5041 ttttctggcc tattttcatt tttacaaacg attggacagg cagtgatgtc ggggattaca
     5101 aatttactgt tagtgattcc agctcctttg tttatcttgt tattgacggt agcagctttc
     5161 tttatttcta agaaacgtcc aggacttacg ttgttcacat taatcggtct atggtttatt
     5221 tataaccaag gtctatggaa tgatttgatg aatactgtca cacttgttct tttatctagt
     5281 gtgatctcga tcatcattgg ggtaccatta gggatcttga tggcaaaaag cagtaaagcc
     5341 caaagtatta tcaaaccgat ccttgacttt atgcagacaa tgccaggatt cgtttatttg
     5401 attccagcag ttgcattttt cggtatcggg atggttccag gggtatttgc ctcagtcatc
     5461 tttgctctgc caccaaccgt tcgttttacg aatttaggga ttcgacaagt tcctaaagaa
     5521 ttagtagaag cttctgattc attcgggagc acaagtcgtc aaaaactatt caaactagaa
     5581 ttgcctttag ctaaaagtac gatcatggca gggatcaatc aaacaacaat gctgtcactt
     5641 tcaatggtcg ttattgcctc tatgatcggt gcacccggct taggacgcgg cgtattatca
     5701 gcattgcaaa gagcacaagt cgggaatgga tttgtcaacg gggttgcatt agttatctta
     5761 gcaatcatca ttgaccgttt tacacagcat ttgaaccagc caaacaataa aaaagctgca
     5821 ggagcaacaa cacagaaaag taaaaaacgt caaggactga tcattggtgc tgtagtagta
     5881 gtgatcttag gtgcgattgg tatagggagt ttttcatctg ccaaagaaac aaaacgtatc
     5941 aacttatctt atgttgaatg ggatacagaa gttgcttcaa caaatgtagt cggtgaagta
     6001 ttgaaacaaa tgggctatga tgtaacgatg acgccattag ataattcaat catgtggaaa
     6061 tctgtttcaa atggcgagtc agatgccatg gtttcagctt ggctacctaa aactcatggt
     6121 tcacaatatg cacaatataa agatcaagtc gaagacctag gtgccaattt gacaggagca
     6181 aaagttggtt tagcagtgcc agcctacatg gatgtaaatt cgatcgatga gttaaccgat
     6241 caggcaggta agaaaatcat aggaatcgag ccaggagctg gtgtagttac ggctgctgaa
     6301 aacacgatcc aaaaatatga caacttgaaa gactggaaag tagagacatc ttcttcagga
     6361 gcaatgaccg ttgcactagg tcaagcgatc aaaaaacacg aaccaattgt agttacagga
     6421 tggacacctc attggatgtt tgccaaatat gatctgaaat acttagaaga tcctgaaaac
     6481 ggaatgggaa gcgaagagca gatccatacg atggttcgaa aaggattgaa agaagaccag
     6541 ccagaagctt acaaagtatt agacaatttc cattggtcag aaaaagatat ggaaaaagtt
     6601 atgttggaaa tcaacaatgg aaaagatcca cagcaagcag caaaagactg gatcaaagag
     6661 aaccaagaat tagtcgaaag ctggaaaaaa taaaaagaca gacacgaaat aaatggagct
     6721 tccaaaattg gatcaaatat ccaaatttgg aagctccatg ttcgtctcta tcttttttgt
     6781 ctataaaaaa agagaatgcg tagctcatct tcgaaacgtt caaagacgcc gacaaactat
     6841 tatgaattta tgggtgcgag tcaaagagat cgatcgtagg tatgaaagac taatgtgatt
     6901 aaagtttatt atgtacgaag tgcattctca aa
//
LOCUS       DACDHJ010000126         6891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDHJ010000126 DACDHJ010000000
VERSION     DACDHJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.128"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..1059
                     /locus_tag="IV223_001921"
     CDS             37..1059
                     /locus_tag="IV223_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8712199.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1070..3274
                     /gene="topB"
                     /locus_tag="IV223_001922"
     CDS             1070..3274
                     /gene="topB"
                     /locus_tag="IV223_001922"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8712200.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3520..4113
                     /locus_tag="IV223_001923"
     CDS             3520..4113
                     /locus_tag="IV223_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8712201.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4129..5028
                     /locus_tag="IV223_001924"
     CDS             4129..5028
                     /locus_tag="IV223_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8712202.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5031..5516
                     /gene="ssb"
                     /locus_tag="IV223_001925"
     CDS             5031..5516
                     /gene="ssb"
                     /locus_tag="IV223_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8712203.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5882..6142
                     /locus_tag="IV223_001926"
     CDS             5882..6142
                     /locus_tag="IV223_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712204.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            6583..6789
                     /locus_tag="IV223_001927"
     CDS             6583..6789
                     /locus_tag="IV223_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8712205.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT         2488 a         1201 c         1418 g         1784 t
ORIGIN      
        1 cccgattggt tgattgatta tgcagaaaaa gaaggattga ttgccgaact aaagccaaaa
       61 catgaacggc aaaatttctt agttcgagac gatcgtttgg atcatgcggt cgccttttta
      121 tggaaagatc cacagacaaa agagacagta ggggcgagtt atcaaggaac gttcattgat
      181 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
      241 catggcttta acctaaagat tggtgatcct aaacagttaa aattttttga gagtagtatt
      301 gatttactta gctacgctgc actgaatcgt gatcagttaa acgatacgtg gttagtctct
      361 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
      421 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
      481 catattttct acgaaaaaga acaattaatg ggcgcagttg atccatttac gaatcagaaa
      541 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caagagaata caaagtcatt
      601 tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag ctattatggc catacacaag
      661 acagaaaaca atctgcaatt aacgaaccaa ctggtttccg ctcacaaagt caatgcgtct
      721 tttggtcagc agctttctgt taacgattca atagaagtca ttagtttgaa agatatttgt
      781 agagaagtcg caaaagaatt aaaagattgt gaacgctcga atggtacgta tgattttgat
      841 cgtttttatc aaggaaaagg ggatattaat tctcagattc ttttctctta taaagcagaa
      901 caatactaca aaggctataa aaatcatgaa catgaatttg tacctgaagt gaaaaaggat
      961 tggaacgatc aattgaagca tgagatccat caacaggaaa ttagaaagca aaaacgtgcg
     1021 atgttgtttc aacaaggcag acaacaagaa agggagtgag atcgtcacga tgaaaactgt
     1081 cattttagca gaaaaaccgt cacaagcgaa ggcctatgcc gatagctttt ctaaagccac
     1141 ccgaaaagac gggtattttg aaatccaaga tcgattgttt tcgggtgaaa ctgtgatcac
     1201 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaattggaa
     1261 acaatggtca ttagagcatt tgcctatttt ccctacacat tatcactatc atgttcctaa
     1321 agataagaaa aagcaattca acgtggtgaa acagcaactt caatcggccg acaccattat
     1381 tattgcgaca gatagcgatc gtgaagggga actgatcgct tggacgatca ttcaacaagc
     1441 aggcgctgat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
     1501 catttatcag ggattccagc aattacgaga cgcagaagag acctatccca aatttgaaga
     1561 agcacaagca cgccagatcg ctgattggtt gatcggcatg aacggcagcc cactgtatag
     1621 tttactcttg cagcaaaagg gcataccagg aagtttttcc ttaggcagag tccaaacccc
     1681 aacgctttac atgatttatc agctgcagga aaagattaga aacttccaaa aagagcctta
     1741 ttttgaaggg aaagcccaag taaccgctca aaatggcata tttgatgcga agcttgatcc
     1801 caatgaaacc caagcgacgc aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
     1861 agtaggaaag cagccaggca cgattcttga agtcgaaacc gagaaaaaaa gcgcggcaag
     1921 tccacgtctt ttttctctat ccagccttca gtctaaaatg aatcagctca tgaaagctag
     1981 tgccaaagat actttggaag ccatgcaagg gttgtatgaa gggaaatatc taagctatcc
     2041 acgaacagac accccttata ttacagaagg agagcatgcg tatctgttgg atcatttaga
     2101 tgaatacaag cattttttaa aggctgaatc attccctact cctattcata caccgaatag
     2161 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacagt
     2221 tatgacagaa gcaacgtatg aaaagctgac acctttacaa caagcgattt atgatcaagt
     2281 tttaaagacg acggtggcca tgtttgcgga aaaatatacc tatgaagaaa ctacgatttt
     2341 gacacaagtc cagcagcttc agctaaaggc agtcggaaaa gtaccagtag atctaggttg
     2401 gaaaaagcta tttggcaaag agagcgacag aaaagagaag gaagaagaac cgctacttcc
     2461 taaagtaaca aaaggagaaa cggtgacggt agatctacaa atctttgaaa aagaaaccaa
     2521 gccaccacaa ccttacacgg aaggtacact aatcaccgcc atgaagacag ctggtaaaac
     2581 cgtggatagt gaagaagcac aatccatatt gaaagaagtg gagggaattg gtacagaagc
     2641 gacacgagca aatattattg aaaccttgaa gcaaaaggaa tatatcaagg tagagaaaaa
     2701 taaacttgtg gtgacgaata aaggaatttt gctttgtcag gctgttgaaa aagaaccgct
     2761 gttaacaagt gccgaaatga ccgccaaatg ggaaagctat cttacaaaga ttggtgagca
     2821 aaaagggaca caagctactt ttctagcaaa tatccaaaaa tttgtttccc acttgttgga
     2881 agtcgtccca ggacaaatcc aagccactga ttttggagca acgttacaag aggtgaaagt
     2941 agccagtgaa aagcaagaag cggcgcgtca tttaggatct tgtccaaagt gccaggaaaa
     3001 agaagtcata ctttatcaga aggtggcgac ttgcacgtct gaagcctgtg attttaaatt
     3061 gtgggtaacg attgccaaaa agaaactcac agcgacacaa ctgaaagaga tcattcagaa
     3121 cggccgaaca agtcaaccag tcagaggact aaaaggtcag aaaggttcgt ttgaagcaac
     3181 gatcgtttta aaagaagact ttacaacagg atttgagttt tctgaaaaga agaagaccac
     3241 ctacaaaaga aaagtgagac gtaaaacgag ttagaattga cggagggaaa aacgatgaga
     3301 aatacaacgc ataaagaaca agaaatcgaa aatcaaaaag ccaaaatacg ttcagagcag
     3361 gagaaaatgc gtgccagcaa acgaagacta cagcaattga agaatcaaca acggattcaa
     3421 aaagaacttg aattagatcg ataaatcctc ctgttgttaa tattaaacta acatgttata
     3481 gttatgttag tttaatatta acattcgaga gggaaagcga tgaaagataa agtcaagtgc
     3541 atttttgaga aatacaatgg taatctatcc ttgaaggatg ctagaaatga agggttatca
     3601 cctatgacgt tagatcgttt agccgaaaag gacaaaatag aaaaaatagc atcaggtttt
     3661 tataccttaa aagatagttt tgtggatgaa ttatatctcg ctcaaagtat ctatactaga
     3721 ggaatcttct ctcacgagac agcgcttgat ctttttcaag taggaacata tatccctaaa
     3781 aaaattaata tgatgtttcc taaaggatac aatgtttcga aagagaattt agatcagtat
     3841 gccattcaac cgcattacac gaataaagaa aactttgaac tgggaatcac agaggcagaa
     3901 agcttttacg gcaataaaat tttcgtctat gatttagagc gaacgttatg tgatatgtgg
     3961 aatcccagat ataaagcaag cgtagaagta aaacaagaag cattaaaaga atatatgaca
     4021 agtaccagca gaaatacaaa taagctaaga agatacatga atattttaaa atcaccaaaa
     4081 gaaatgactt tgtatatgtt gccactttat tgagaggagt aaaggtgaat gacaccgaca
     4141 caatttaaag cgaaaacaag aaatgttgca gctgagaaac atttaaaccc acaaattgtt
     4201 caaagatatt atatgatgga aaaattatta gaatgtattg ttgaatcgcc ttatcatgat
     4261 gattttgtat taaaaggggg atttctgatc ggatcaaaat acgggttaga aaaccgttca
     4321 accgttgata ttgatacgac ttatcgaaat tcgaaactaa caaaagaaaa attagaaact
     4381 gttttttctg aattaacagc tgctcctaca aaagatggca ttcggtttga attaaagggc
     4441 ttaacagaga cgagggaagc cgattattac ccaggttttc aagcaaagtt tattgctttt
     4501 cttgaaaatg cacgtgtacc ttttaaatta gatattactt caggtgattc tattattcca
     4561 gcagcattga tatatgaaca taaattgatg tttgaagata aaacaattca tattccagct
     4621 tatcctacgg aacaaatttt agctgaaaaa ttacatgcca cgtttagttt cggaatagat
     4681 aattcacgta tgaaagattt ctatgatctg tatatgatcc ctaaactaga acgtatcgat
     4741 gagataaatt tgtataagag tgttcaagga acatttaagg aaagaaataa tcaatcagtg
     4801 tttaagaatt tgtataatca ggaaatgcct caattgatga agaatgaggg attgagggag
     4861 aaatggaaaa gatatcagca agaaaactat tttgccaaag atatttcttt cgaatctacc
     4921 ttaacttctg ttgattcgct gatgcaatca attgtcgagc aagaggaagt agaacgaaaa
     4981 caaaatttta agagatcaca acaaatgaat caagaaatgg agagataaag atgatcaata
     5041 atgtaacttt agtaggtcga ttaaccaaag atcccgattt acgttacacc gcaagtggca
     5101 ctgcagtgac cacttttacc ttagccgtta accgtaattt tactaatcaa agtggcaatc
     5161 gagaagctga ctttatcaat tgtgtgattt ggcgcaaacc agctgaaacg atagcgacat
     5221 tagctaaaaa aggcagcttg atcggcgtcg tcggacggat tcaaacgcgg acttatgaca
     5281 accagcaagg acagcgtgtc tatgtgactg aagttgtggc cgataatttc caactattag
     5341 aaagtaaagc agcgacagaa agccgtatgc acgctgatca atcgtccagc agtccaagcc
     5401 ctaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
     5461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttctaagtag
     5521 cattttctat tggttgcatt tgctacggtt tatgttataa tttagacaac aaaaagaatt
     5581 ttcaatctat aaccaaaaac cactagctgg cgggctagtg gctttctttt acgtttctat
     5641 tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc aagccatttg
     5701 cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc
     5761 aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta
     5821 tagcataatt gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt
     5881 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt
     5941 ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga
     6001 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     6061 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
     6121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
     6181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     6241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     6301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
     6361 tctcaccaaa aggttcgttc ctgttcgtta tctctttatt gatttgccag accggcaaca
     6421 agttgccttt tatcaccaaa aagcgacaag gacaagggta aatggcacgc tgcgcgccct
     6481 tgtccttgtc gcttttcgtt gatttattct ggccaacaaa tcaataaaga aagaactaac
     6541 gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa acatgcgaaa gaaatttgat
     6601 tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca tactgttgaa
     6661 tgcgattgtg gagaaagagc gaaatgtatc aaaagctatc gtttgtatca atgtccaaca
     6721 tgcgggaaaa aatatacctt gtcttatggc gattacattt tgattgatga gaaaggaaag
     6781 aaacgctgat gaaaacacaa cacaaattat tgactgtaaa agaagtcgtt caaattttgg
     6841 tggacaaaga agtcatttct gaaaaagtag cagaagcttt acttattccg c
//
LOCUS       DACDHJ010000127         6839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDHJ010000127 DACDHJ010000000
VERSION     DACDHJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.129"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(205..735)
                     /locus_tag="IV223_001928"
                     /pseudo
     CDS             complement(205..735)
                     /locus_tag="IV223_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            807..2099
                     /locus_tag="IV223_001929"
     CDS             807..2099
                     /locus_tag="IV223_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8712206.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2311..3153)
                     /locus_tag="IV223_001930"
     CDS             complement(2311..3153)
                     /locus_tag="IV223_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712207.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(3190..4656)
                     /locus_tag="IV223_001931"
     CDS             complement(3190..4656)
                     /locus_tag="IV223_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8712208.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(4712..6556)
                     /locus_tag="IV223_001932"
     CDS             complement(4712..6556)
                     /locus_tag="IV223_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8712209.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
BASE COUNT         2092 a         1422 c         1139 g         2186 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taacatttcc atttcattta
      121 cctctttttc gtttaattat actaaagttc tatccaagcg acaagctgtg tataataaaa
      181 atatacccgt ggtgctagtt aattttaata ccctaaagaa tatctcaaag tcccttcaaa
      241 atgttgctgt gctcggctta aaagaatact gtacgataat aatatgcttt caaacttact
      301 ttctagccct tgaatagaac gtgatttgaa attttgaaaa cctagtcttt cgagagaaga
      361 aaaaacagtt tctactgctt ttctttgtct agtaagtaat cgatggtcta tcttccctag
      421 tttatccatg ttttttcgtg acggaactaa gactgtaatg cccttaaggg caagcttttc
      481 atggatttta tggctgacat agcctttatc tccaaggata tttggaaggt ttgcttcttc
      541 cgataaagtc tctaaaactt tcacatcatg aacactagga tccgtgattg aataagtaac
      601 tggaaagcct gttttagtga cgataatatg aattttaaga ccataaaaat aagcgttttt
      661 cgttgagtta tagcccctac agctctatac tttttgatgt tggtggatga caccagcatc
      721 ttttttattt tccaaaacat atagaaaaga caccttgaaa tcctttagaa ttaatttggc
      781 gaaccaaccc aaaaggagga atcaagatgt cttacactca ccttatcaaa gaaacattag
      841 atattctaga cttatcagtt acttttaacg aaaattgtct cacaaaagaa aaatacaaag
      901 ggcaaatctg tcatatttat cgtggaaact tgatttatac agcccaagaa tgcatccact
      961 gtaaacacca gattgcttca gatattgtgc gttggggtac aacaaccgtc cggttgttaa
     1021 tgaacgatgt ttctgaatgc cgtacctacc tcgaattgaa aaagcaacgc ttcaaatgca
     1081 aagcttgtca gcggaccttt gtcgcagata cttctgttgc gaaaaaacac tgtttcatca
     1141 gtgagaaagt ccgctggtct gtcgtgactc gtctgaaaaa aaatacctca atgacagaga
     1201 tcgccgctca gaaaaatcta tcggtctctt ctgtttaccg tattatgaag cgattttatc
     1261 gtccgttgaa tccttttcgg acaccgttac ctaaggtact ttgttttgat gaattcaaat
     1321 cagttcgggg tgtttcaggt gctatgagtt tcatcatgat ggatggacaa acacagcgct
     1381 tgttggacat tgtcgagaac cgacaactgc cttttttaaa acgttatttt agtcattttt
     1441 cacgagaaat tcgtgaagcg gtcgaatgga tcgtgattga tatgtacgct ccttatgtat
     1501 cgctcgttaa aaagctgttt ccaaaagcac agctgattat tgaccggttt cacatcgtcc
     1561 aacatattgg tcgtaccttt cgcaatcatc gaatcaaaga aacgaaccaa ctattaaaaa
     1621 gcaaagaaca aaaacactat caattaggta agcaattaaa aagatactgg aaacttcttc
     1681 aaaaagatga acgcaagctt gattatactc gacgcctttg gcgacctggc ttcaaagctc
     1741 atttaacaga gaccgatatt gtggatcgct tactgaaagg gagccctgct ttgcgtgtag
     1801 gctatcagtt atatcaagac tttctttacg ctgtaaagga acgagactac gtatcgtttg
     1861 aagaactact taccaataat atcatgcttc ccgaaggcta tcagacgacc ttgagaacgt
     1921 tccagaaatt tctgccacaa atcaaaaatg ctttacaaca aagttattca aatggcccac
     1981 ttgaatgtct gaacaaccat atcaaagtgt taaaacgaaa tgcatatggc tttcgtagct
     2041 tttataattt caaattgcgc atcatgattc gtcatggcaa cgcattgatt ttcaactaaa
     2101 aaagatcaag aggaactgat ccacttgatc tccaattatt ctaagatttc tctttaccaa
     2161 catcatttga caaagagccg agatttttta ttatcttacg ttaatccttt ttaaatgtct
     2221 cttatagata acaaacagct ggaaatcacc ttgtttttac tataatccat ctctaaagat
     2281 tttaaaagca tcaatagtat tactgagcgc tcacccatta gcatgtttca tcaaccgatt
     2341 gatgtggaca cctaggtata caacttcatc gttcggaatt gttttatgat aaactttttc
     2401 aatgtagtct ttgatcttag ctgcgctttc catggcagac gggtataaga tccacatttg
     2461 attgtagaat tcttcctcac catcatccaa caaagcatca gaataatatc tttctacaaa
     2521 gaaacgtaca tgtgtaatga agcgggcata atggatcgaa ttagtatcga tatctttctg
     2581 caacgaatac cggaccaaat tgacgatccc gccgatcatt tttgcatact tgaatccagc
     2641 gccgctatca ggatcttccg attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc
     2701 ctctttagga agatcaattc catattgctc gttcaaaagg gtgagtgcgt acttcccaat
     2761 ttgaaattct ttgggatagt agatttttat ttcccagtac aaacggtttg caatatttac
     2821 ccctttttgt gcgcgttcaa ctgcaaaatg caggtggtct gttaaggtta aatagactcc
     2881 aggattcagt tttttatcta atcctttttc tgcttcaaaa acgatagtac gagtgatttc
     2941 aaaaaattca ataggtattt cttcaactaa ctctaatagt tgtgaagatt tcttttcttc
     3001 aaaaggcaga aatattttat ctactttatc tcctgatatt tcatccccag ctttctgtcc
     3061 aaagcctatc cctttaccta atgcaatcat ttctttgcca ttttcttcaa caagtaccac
     3121 acttgaattc aatacttttt ttattgaaat cactcattat ccccctatcg ctgcttaaaa
     3181 agatcgagac tactcgaata aggatgcacc gtttgtttcg atcacttctt tgtaccagtt
     3241 gaacgaatcc tttttcaagc ggttatatgt cccatttcct tcatcgtctg catccacata
     3301 aataaatcca tatcgtttag acatttgaga agttcctgca ctaactaaat caattggtcc
     3361 ccagctagta taagctatca aatctacacc ttcatcgatt gcttctttca tttgttggaa
     3421 gtgtgcttta aagtagtcga tgcggtacgg atcatggatt ttaccatcta ctacctgatc
     3481 gactgctccc ataccatttt ccacaatcat caacggtact tggtaacgat cataaagttc
     3541 gatcaacgaa attttcaaac cttttggatc gatttgccag ccccaatctg ttgaaggcaa
     3601 atgaggattc ttaaccccta aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc
     3661 tgattctgcc atggacatgt aatagctgaa agaaacaaaa tctacggtat gtttctttaa
     3721 aatttctcga tcttcatctt ccattttaat ctgaattccc cgattttcca aatccttttc
     3781 gattagagca ggatattctc ccttaacttg aacatctgca taaaaattat tttctaagtt
     3841 ctttttcaag gtcagctcaa cgtcttctgg tgcgcaagtt aatggataag tcatcaattt
     3901 cgttaacata caaccaactt ggcttcctgg aattttttca tgagcatctt ttgtcactaa
     3961 cgcactcgca acaaattgat gatgcaatgc ttgatagata tcttgagctt cttgaccttc
     4021 tgcgcttttc tcttggataa ttccagctgt tgtaaatgga tgacggtgga tgctgtctat
     4081 ctcattaaat gtcagccaat attttacgta tttgccgaag cgatcaaaac aaacgttagc
     4141 gaagcgaaca aaatcatcaa ttactcgacg ctccacccag ccattatatt taactgctaa
     4201 agctaatggc atttcataat gagatagtgt cacaatcggc tcaattccta aacgttgcat
     4261 ttctttaaag acgttttcat aaaatgcaac ccctttttca ttcggttcca gctcttctcc
     4321 tgtagggaac aagcgagtcc aagcaataga gaggcgcaag gttttaaagc ccatttcggc
     4381 aaataaagct aaatcttctt tatagtgatg ataaaaatca acgccccttc tttttgggta
     4441 ccatttatca gatttgtctt cactagcttc ttttatagac tgcagggtga catgcatatg
     4501 agcagcaaaa tctttgttgt ctaaatttgg tttataagta gccatatccg ctactgaaag
     4561 acctttaccg tctacattcc aagcaccttc tacttgattt gctgctatcg cgccgcccca
     4621 taaaaatcct tttggaaaac ttgttgtgtt tgtcattatt ctattctcct ctattccttc
     4681 attcaatata ttttttttca ctatcttgtg tttaagctat cggactgata gctaagagat
     4741 cactatcatt ggttaccaat ttttctaggt taaatgaacg gatagtgtaa ttatccttat
     4801 tggttaccac acagataact gtggggtcga atcctgcttc tttgattaaa tcaatatcaa
     4861 atgcaatcag cttttgtcct ttggctactt tttcaccaga acgaacaaat gcctcaaacc
     4921 cttttccttc taactgaaca gtatcaatac caatatgaaa aagaagttct gcaccattag
     4981 ccgtttttaa accaatagca tgcttcgttt caaataccat tgtgatttct ccatcttcag
     5041 gagctactaa tatcccttct tcaggaatga ttgctactcc atcacctacc ataccactag
     5101 aaaaaacagt gtcttttact tctgatagtg agattacttt tccattcacg ggacttgaaa
     5161 gaacagcatc tgaaacaacg gccgtttcca tcttagtttc aacttcattg acaacttctt
     5221 tatctttata aagaaccaac gctgcagcaa aagcaaccac aaatgaaatc cccgatactg
     5281 caaatgccca aaccagattc attgggtcag ctttgtcagc gaacatcgtg atacttgcaa
     5341 tcccaggacc cactaaagca aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga
     5401 tcaggctaga aaacataacg gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa
     5461 gagctggttc agtaattccg aataatgcag atatacccgc agaaacagcc gtagatttca
     5521 attttttgtc ttttgttttg atactgacag caaagcatgc gccagcttct gaaatattgt
     5581 gtgccaatga agccgggaga taaagaagtt ctgcacccaa ttcactcatg gatgaaacag
     5641 cgtaagggat cattgcttta tgcatacccg ttacaaccat taatggcagt acagctgcta
     5701 agactccggt tgcaacccat ccaaattgat tgtacaaaga cacaatgata gaagagaaag
     5761 ctgttcctac attataacct aatggtccta gaagcaacaa agcaatagga actgtaatca
     5821 ataagctcat cataggtaca aagaaaatac gaattggttt tggtgaaact tttgtaaacc
     5881 atttttcaag ttgagcataa aataaaacag ttaagatggc tgggaatact tgagatgcat
     5941 acgctatatt atccaatcca aaagataaga atgtagcacc ttccgccaac atagctgtca
     6001 tttcaggaag gatcaatgca ccgacagcag atactgcaac caattgattg actttcaatt
     6061 tattagccgt agtcattgcc accagaatag gcaagaaata aagcggtgcc ccgccgacca
     6121 aatttaaaat ctgataggtt tgagattggt cactaatcag ccctaagata gaagctagta
     6181 gcaacaagga cttcaatact cctccacctg caatcgcagg aattaaaggt tggaaaatag
     6241 aaataataaa atctaataga actgaaccta atttttgttt ttgttttggt gcttcttcag
     6301 tatttgaaac attgtcacct aataacgctt tcacttcatc atacacttca acaacatcgt
     6361 ttccaatgat tacttggcat tgcacatttt ggcgaacgcc cataacgcca tccacttttt
     6421 ctaactggct catatttacc ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc
     6481 aatgctccaa atgggatatg ttttcttccc caccgatgta ctgcaatact tcttctgctg
     6541 tttgtttgta attcatggtg tttccctctt ttcttttttg agtgatgaac ttttgagtcg
     6601 aagtggccat cctcaacaca aaaaacaggc agaaactaaa taaattgaca acaaggcaaa
     6661 cgtgtattga aacgcataca ttgttattca acttatttag gtcctgcctg cattaccagt
     6721 cacatgcctg tttaagttat attcaattta acatattaga atgtaaacgt aaataataaa
     6781 atgatttttt aatcattttt tcattttatt cgttcaatat agttttcgtg ttctaaaag
//
LOCUS       DACDHJ010000128         6803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDHJ010000128 DACDHJ010000000
VERSION     DACDHJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.130"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..213
                     /locus_tag="IV223_001933"
     CDS             <1..213
                     /locus_tag="IV223_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8712210.1"
                     /translation="PTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAIPLTGSNGF
                     QTYVLISCLLLGAGALSAVVYFKKKA"
     gene            226..963
                     /locus_tag="IV223_001934"
     CDS             226..963
                     /locus_tag="IV223_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8712211.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFLLFLFIKK"
     gene            977..1804
                     /locus_tag="IV223_001935"
     CDS             977..1804
                     /locus_tag="IV223_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712212.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            1814..2062
                     /locus_tag="IV223_001936"
     CDS             1814..2062
                     /locus_tag="IV223_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712213.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            2093..4204
                     /locus_tag="IV223_001937"
     CDS             2093..4204
                     /locus_tag="IV223_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8712214.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(4242..6299)
                     /locus_tag="IV223_001938"
     CDS             complement(4242..6299)
                     /locus_tag="IV223_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8712215.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(6413..6625)
                     /locus_tag="IV223_001939"
     CDS             complement(6413..6625)
                     /locus_tag="IV223_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712216.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
BASE COUNT         2284 a         1111 c         1264 g         2144 t
ORIGIN      
        1 ccaactggtt accaaaaatt aacagaagat attgcattta aagtaaacaa aaactcattt
       61 aaagaggaaa atcgaatcac tgttaaaaat aatcaaaaag cagctattcc attaacaggt
      121 agtaatggtt tccaaacgta tgtattgatc tcttgcttgt tgctaggagc aggggcactt
      181 tctgcagttg tttactttaa gaagaaagcc tagaaggatc agctaatgat tattcgtaag
      241 cccccaaaaa gaagagtcat gcacaaactg atgatatttt tttggttgct gtcggcagta
      301 gcagtctatt taattccagc tgtggaaaca aatgtagcga aatttcaaca acagcaaaga
      361 attttagcca ttgaaaatct tgaaaaaaca gattcttctt tggatgttgc gaaaatagag
      421 ttaggcgatc ctgttggtgt tcttacaatt ccaagtattc ctttaaaact accaatttat
      481 gacggaactt ccgacaaaat tttagagaat ggtgtaggga ttacggaagg aactggcgat
      541 attacaggag gaaacggtaa aaatccttta atcgcggggc acagtggact ttataaagat
      601 aatcttttcg atgatttgcc ttcagtaaaa aaagggcaaa aattctttat taaggtaaat
      661 gaagaacaac atgcctatca aatttatcga attgaagaag ttaaagcaga agaattacaa
      721 aaaaattatt tagattatct catgcctgaa aaagaccaag ataggatcac gttaatgaca
      781 tgcacgccaa aaggtgttaa ctcacaccgc tttttagtgt atggtgaacg agtgtcattc
      841 agtaaagaag atttaaaact acagaaaaaa gcaaaaaata ggaaatcaat ttcattggat
      901 tggcgattag cactgctatt cgcttttttt gcgttcctat tatttttatt tataaaaaaa
      961 taaggaggag atcggaatga ataagagaaa atggctgttt gtttgtgtag cagctctctt
     1021 tgcaactgca gcttactcac agatatcttt tgctgatagc agtactactc caactaccag
     1081 tcagacaaca gatagttcaa caactgcaac aagtgacacg gacagttcga agaacactgc
     1141 agaagaaacg gcagattcaa gtacgagttc tactgaatca gcggaatcaa cgtcagaaag
     1201 ttcttcagag acttcagaaa aactgcaaga agtagaagat acgcaagtaa tcaagcaaaa
     1261 cattcatatt caaaaaatta tttcttcgaa tcgaatcaac aatcaaggaa ggcaagtgac
     1321 tcagcaagaa ggtgtcaatg gtgcaaagtt tattgtctac gatatcacga atatcttaga
     1381 agaaatgaca caagatgata atgagaaaaa tcaatcggta gaaataattg agtcaaaatt
     1441 gaaagatcga gcaaaaaaac tttcatacga tcagttgaaa aaagtgactg aaggagaaac
     1501 ccaaacaatt gatgatcagg aaggcgtgat tgatttttca attgaagtac ctgcaaacaa
     1561 aaaacaagca tactatatcg tcaatgaatc aagccctgaa aacatttcaa atagcgaaga
     1621 tattgtttta ttgacacctg tttcagatga aaagggtgaa tttttgacgg atgtttggtt
     1681 atatcctaaa tcaaagaaat cagagccgaa agaagaaatt aaaaaagttg tatcaactgg
     1741 tgtaaagaaa aacttttttg ataattgttg ggattttttc actcatttat tttttaacga
     1801 ttaggatgaa atgatgaaaa taataaaaat gttagttttt aatctatctt ttttagccgc
     1861 aacaatattg tgttgcggtt tagttttttc tcaattatca atttacccaa gatatcaacg
     1921 attgttcgtt cattttctaa atatccagca tgcttccatg ccactagaag tgattaacaa
     1981 gctcttaatc aatccaattt ttatggctag tttgtttctt cttgcaggat tggtaacgtg
     2041 ggtattagga aaaaaagaat gagaagctta caatcgttta acaaaataat agatggaggg
     2101 cattatggaa aataaaattc attgcactac gggaagagta tttactttag tgttgttttt
     2161 gttccttctt ctttttgcaa ggggaactgc agtccaggcc ttagaaaaag ttgatccgag
     2221 tcaaatcaaa gaaaataata atctggtact ttatcatgat agtgattcat tgagaaatat
     2281 cgaagtaact gggatcaatg gatttaagat actagatgag tattcaacta aaaaacaaaa
     2341 agtacttgct tggccaaaag tcaaagattt gaaaagcacg tcaaaaattg ttgcccatta
     2401 tcaaaaggtg ggcacttatc taggaaaaga aattgattgc gacgtaactt tttctaattt
     2461 tgtagcgcac gctggggtta accaagacgg attagctaaa acaattaatg ggaaaaagta
     2521 ttataattat ttgctctttg ataaaaacat ttatcgaggc tttttcatgc agtcaatcaa
     2581 cgttatgaat gttaaactag attttaagta tgcaggatca aaaacaaaag tagctttgag
     2641 tgaaccacag ccagaaatta attcaaaaat gagatcgatg agagcagctg gtattgagtt
     2701 gaatgataca tttctatcta tttacagttt aaatggacag tatcagcaac gtgtgtcttg
     2761 gtataatgac aataatgaat ctgtcggtga acgtaatatt gatatgagag aatttgttgg
     2821 gtacgaaaaa atggatcgct taccttattt tgtcacaaca gatacagcat gtgcagaata
     2881 caaagctcct gcgttatcaa caaacaattt aacttcaaaa gtagtgggag gacgtgcaga
     2941 aaaggcttat agctcgaatg atcatttcac cgatgttgta ggagctgata cttatcacag
     3001 aagtggtgta acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg gcgcaaatac
     3061 gaatagtatg atgtttagct ttgatactgc attgctttgg acaccacaac catcgaagcc
     3121 tacaaaagaa gtgtttaaca aagctaatac tgaagaggca gcacacaata ttgacaaaaa
     3181 agtgattcca caaggatcag atgtttacta tcatattcac caaaagtttg atgcattaac
     3241 agtcaacaca atgaacaaat acaaatcatt taaaatcact gatacctttg acagcaaaaa
     3301 ttttgatatg gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg ttgaagcgaa
     3361 tgggacttgg aaaaaagctg atacaggagg aaaattaact gtatcaggta atacagtcac
     3421 ttatactgct tctgacagct ttttagctaa tgctgcaaat tacggtaggg actacatttt
     3481 gcagctccat atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg tggctactgt
     3541 aacgtttaac aatgatccga acaaaggaac acaaaaaaca aataaagtag aaaactattt
     3601 ctctcttaaa ccaaataaaa aagtggaaca agatgggaaa gatgttgatg gtcgaaatga
     3661 tggtaaaaaa ggaacgccta cagctccttt aaatgcagga tctgaagtac aatatctagt
     3721 cacacaaaaa tggcatacaa aaggtgtaga cattgcgctc gatcattaca agcaatttag
     3781 tattcaagat ccagtggaag ctcgtttgac gtataaagaa ggttctgcgc gagtgataga
     3841 tatgagtaca ggaaaagata ttacttcaga aggtatacta acctatgata gccattcaag
     3901 aacgctgaaa tgggaagctt ccgctgattt cttaaataac aatcttttag atggacgaga
     3961 aattcaactg atatttacag ctaaaactcc tttgaatgaa gaattggata tcgacaacca
     4021 agcaaaagtt tcagtagaaa gtattgagaa agcaactaat gtagtaacga ttggggtaaa
     4081 acctaactta cctcaggtca ttgtgccaaa aactggatcg aatcatttag tgctaactgt
     4141 tattggaagt atattatttc ttactttagc atcattgggc tatgtagttt taaaactcaa
     4201 ataaatataa aaacaaggct gattttcagc cttgttttct tttatttgaa gattggattc
     4261 gcactaataa atacattatc tacgccaatt ctaccagcag agtcaactac aaaattatga
     4321 cctaatccga gaatactttg gttattataa agtaaatttg cattatgggc agggctattt
     4381 ttccatccta ccatggcatc gatagcacca tcgtctgata aaaggtcatc aatatacagc
     4441 ccagtttgac ttatgttttc tcccttagca ccatagcagc ctaagctttc caataaatct
     4501 tggaaagtac caccaccagg gcgatagtga ctgaagctga cttgtaattc tttagctcga
     4561 atatctgcag cctgttgaac aatatcattg aattgcattg gggcaacacc attttgttgg
     4621 cgataatcat ttaaccaagc aagttctttt tgacgcataa agttacgtaa ttcgtctaat
     4681 gaaatctttt tatatttgaa tgtgattgtt ttattttgat cgacgttatt aatggatcgt
     4741 tggccgtttt tatccacggt aacggtagga tcgtcatttc tgcacaagaa ataattttga
     4801 tcagtcatat ttttccaagg agtagagaag ctatcccctt cgtatactgt ttttgattca
     4861 ctagagagaa cagcgttggt atcagcattt acatgattaa cagtcacatt aaatttacga
     4921 gtgtagttga aaatataatt ttgactttca gtcacattgt ttaaggttac tgaatttgtt
     4981 ggttctaccc gatcgtctaa aacatatcct tgaatagaag gagcagttaa agtgacactt
     5041 tcaccgtgag tgacgttgat tatctgttct ttcaaaatat caactcgccc ctcatttttg
     5101 tagttgttat tgcacaacaa attgatttga tgcacttgat tataattgaa tgtgattgtt
     5161 gtattacctg tgattgtttt gctttgagta tcattaccaa tcactttgta gctaaaatta
     5221 tctttagcac cttcgctata tttaaatgct tttgccttag cagtgtaagt agaaccagaa
     5281 gtcacttttg cttgatctgt agcaatcact tgacctgttt gattgtcaat atggttaatg
     5341 gtaaccatat attcttttgg cgtttcaggt ttgtgttgca accctttaaa tgcagcatca
     5401 agactatttt tagcattgtt tacttgtgtt tgagaaacct tagaatcgtc tagtactttt
     5461 ttagcattgg ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa atctccttta
     5521 gcagttgatt ttactcggtt gtataaagct tgtaattcag ctttattcac agtaggaact
     5581 ggttttttct ttaatccttt gtaagcagaa tctaatttat ttttggcact atccacttgc
     5641 ttttgagtcg cttttgagtc atttaacact ttcttagcat tgtttaaagc ggtttggaat
     5701 gcgttccagg tattgtcggt atagtcacct tttttcgttc ccttaacgct cgtataaaga
     5761 gcttctagag ccgatttatt cactttgggt ttagaggtgt cagcgccata ccaaccgata
     5821 ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca acttgcagtg
     5881 tagtgatgtg agccagattt tacaccagga aggaaaaggc ggtagatcgg tttattattt
     5941 ttttcggcag aacgccaggc aggcccttca taagtccagc cgtaattttt agtcaaccat
     6001 ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa gacctttatt gtacaaacga
     6061 tagacaagat ggccaccgct agtcggtgct tcccaggcgg ggccttcata atttccccat
     6121 tttatcttta ctagatggtc acgttctttt ttgctagagg tgtaaaaatg ttcattatta
     6181 tttggattgt aaaggcgata catgacgtta gttgaagcag cgtcaacaga tccagcaaga
     6241 ctaagtgagg cagcaaatgt tccaatagct aaaacagttt ttggattaaa taatttcatt
     6301 ttttcaactc ctaaatttaa ttaaaaatgt ttacatgatt attatatatc ggttaaccga
     6361 tatagtcaat aaaaaataag aaaagcctta tataattttg gcttttctta ttttatttgt
     6421 tagttgaagt attaatatca acgatttctt cagccaattt atttaatttc atagcattta
     6481 tgaatctcat gttgtctata ctagagcgtt tgttggcaag gtcacttact gtagttaagg
     6541 gtacgccaga ttctatggat atagaacgaa ctgatttacc actatcaaac agcaaccatt
     6601 cgattttctt tttatcagcg tacatttcaa tgctccttct tgttttttct ttattatagc
     6661 tcttttttgt aatgaatcac aatatcaaga gaaaaatttt ctatagaaag gtggattttt
     6721 atgagtattc gttctccgtg tatccaaatg aggtttgttg ctactttcgt tatttgatat
     6781 actaaaaata aacaacgaaa gaa
//
LOCUS       DACDHJ010000129         6684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDHJ010000129 DACDHJ010000000
VERSION     DACDHJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.131"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(93..551)
                     /locus_tag="IV223_001940"
     CDS             complement(93..551)
                     /locus_tag="IV223_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8712217.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(564..1889)
                     /locus_tag="IV223_001941"
     CDS             complement(564..1889)
                     /locus_tag="IV223_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8712218.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1906..3318)
                     /locus_tag="IV223_001942"
     CDS             complement(1906..3318)
                     /locus_tag="IV223_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8712219.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3335..4099)
                     /locus_tag="IV223_001943"
     CDS             complement(3335..4099)
                     /locus_tag="IV223_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8712220.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4203..5078)
                     /locus_tag="IV223_001944"
     CDS             complement(4203..5078)
                     /locus_tag="IV223_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712221.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5333..5417)
                     /locus_tag="IV223_001945"
     tRNA            complement(5333..5417)
                     /locus_tag="IV223_001945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5381..5383),aa:Leu,seq:caa)
     gene            complement(5435..5506)
                     /locus_tag="IV223_001946"
     tRNA            complement(5435..5506)
                     /locus_tag="IV223_001946"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5472..5474),aa:Gln,seq:ttg)
     gene            complement(5516..5588)
                     /locus_tag="IV223_001947"
     tRNA            complement(5516..5588)
                     /locus_tag="IV223_001947"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5553..5555),aa:His,seq:gtg)
     gene            complement(5650..5734)
                     /locus_tag="IV223_001948"
     tRNA            complement(5650..5734)
                     /locus_tag="IV223_001948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5698..5700),aa:Leu,seq:caa)
     gene            complement(5755..5825)
                     /locus_tag="IV223_001949"
     tRNA            complement(5755..5825)
                     /locus_tag="IV223_001949"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5791..5793),aa:Cys,seq:gca)
     gene            complement(5871..5942)
                     /locus_tag="IV223_001950"
     tRNA            complement(5871..5942)
                     /locus_tag="IV223_001950"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5908..5910),aa:Gln,seq:ttg)
     gene            complement(5954..6026)
                     /locus_tag="IV223_001951"
     tRNA            complement(5954..6026)
                     /locus_tag="IV223_001951"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5991..5993),aa:His,seq:gtg)
     gene            complement(6029..6102)
                     /locus_tag="IV223_001952"
     tRNA            complement(6029..6102)
                     /locus_tag="IV223_001952"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6068..6070),aa:Trp,seq:cca)
     gene            complement(6109..6189)
                     /locus_tag="IV223_001953"
     tRNA            complement(6109..6189)
                     /locus_tag="IV223_001953"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6153..6155),aa:Tyr,seq:gta)
     gene            complement(6191..6266)
                     /locus_tag="IV223_001954"
     tRNA            complement(6191..6266)
                     /locus_tag="IV223_001954"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6231..6233),aa:Phe,seq:gaa)
     gene            complement(6302..6374)
                     /locus_tag="IV223_001955"
     tRNA            complement(6302..6374)
                     /locus_tag="IV223_001955"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6339..6341),aa:Asp,seq:gtc)
     gene            complement(6384..6456)
                     /locus_tag="IV223_001956"
     tRNA            complement(6384..6456)
                     /locus_tag="IV223_001956"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6421..6423),aa:Val,seq:tac)
     gene            complement(6472..6543)
                     /locus_tag="IV223_001957"
     tRNA            complement(6472..6543)
                     /locus_tag="IV223_001957"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6508..6510),aa:Glu,seq:ttc)
     gene            complement(6558..6647)
                     /locus_tag="IV223_001958"
     tRNA            complement(6558..6647)
                     /locus_tag="IV223_001958"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6611..6613),aa:Ser,seq:gga)
BASE COUNT         2009 a         1473 c         1089 g         2113 t
ORIGIN      
        1 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
       61 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
      121 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
      181 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
      241 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
      301 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
      361 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
      421 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
      481 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
      541 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
      601 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
      661 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
      721 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
      781 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
      841 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
      901 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
      961 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
     1021 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
     1081 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
     1141 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
     1201 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
     1261 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
     1321 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
     1381 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
     1441 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
     1501 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
     1561 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
     1621 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
     1681 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
     1741 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
     1801 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
     1861 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
     1921 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
     1981 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
     2041 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
     2101 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
     2161 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
     2221 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
     2281 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
     2341 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
     2401 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
     2461 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
     2521 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
     2581 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
     2641 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
     2701 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
     2761 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
     2821 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
     2881 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
     2941 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
     3001 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
     3061 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
     3121 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
     3181 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
     3241 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
     3301 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
     3361 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
     3421 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
     3481 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
     3541 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
     3601 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
     3661 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
     3721 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
     3781 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
     3841 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
     3901 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
     3961 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
     4021 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
     4081 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
     4141 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
     4201 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
     4261 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
     4321 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
     4381 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
     4441 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
     4501 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
     4561 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
     4621 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
     4681 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
     4741 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
     4801 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
     4861 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
     4921 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
     4981 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
     5041 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
     5101 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
     5161 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
     5221 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
     5281 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
     5341 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
     5401 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
     5461 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
     5521 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
     5581 acattcgcca tcataaaaga tgttgtttaa tttctcaata aaaaactaag atgaagacat
     5641 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
     5701 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
     5761 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
     5821 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
     5881 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
     5941 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
     6001 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
     6061 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
     6121 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
     6181 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
     6241 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
     6301 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
     6361 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
     6421 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
     6481 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
     6541 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
     6601 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
     6661 ctcaatactc tttctcaaaa tgag
//
LOCUS       DACDHJ010000130         6680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDHJ010000130 DACDHJ010000000
VERSION     DACDHJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.132"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(283..2988)
                     /locus_tag="IV223_001959"
     CDS             complement(283..2988)
                     /locus_tag="IV223_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8712222.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3294..4241)
                     /locus_tag="IV223_001960"
     CDS             complement(3294..4241)
                     /locus_tag="IV223_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8712223.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4431..4700
                     /gene="rpsN"
                     /locus_tag="IV223_001961"
     CDS             4431..4700
                     /gene="rpsN"
                     /locus_tag="IV223_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8712224.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4876..5238)
                     /locus_tag="IV223_001962"
     CDS             complement(4876..5238)
                     /locus_tag="IV223_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8712225.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5249..6370)
                     /locus_tag="IV223_001963"
     CDS             complement(5249..6370)
                     /locus_tag="IV223_001963"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8712226.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6386..>6680)
                     /gene="acpS"
                     /locus_tag="IV223_001964"
     CDS             complement(6386..>6680)
                     /gene="acpS"
                     /locus_tag="IV223_001964"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="holo-[acyl-carrier-protein] synthase"
                     /protein_id="HAP8712227.1"
                     /translation="EEKPKFIARILTSDEMKLFQSLPFHRQVEFLGGRYACKEAFSKA
                     WGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSITHTNETAFAQIILED"
BASE COUNT         1753 a         1439 c         1240 g         2248 t
ORIGIN      
        1 tactcgaagc atagcgcagt tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat
       61 ctgcggtaat aagcccaacc aaattaaaaa cataagaagc tgtttttatt tggtcgttct
      121 tattacttga tgcagagcag cttttttaca acctcttggt acacggtgtt caatcagctg
      181 accctcggta ttagctcaaa aaaacggcaa aatatgagga actatttttc catttttttc
      241 tctaatactc aggtcaaaac actgatttca caacctcttt ttttaatgaa aaatatggct
      301 ctcatgcgcc gcttctttga atttcgtcat tttttcttcg atgaacggat agattgccgt
      361 tcctacgatt ccaaagacaa caaacaaacc gatcatgatc cagattgctt tcgtagcatt
      421 tggccaataa attcctcctg cagattcccg taacaagttt accgcatgag taaatggcaa
      481 gaacggattg atcatttgga agaatttccc tgagacttgg atcggatagt tcccgcctcc
      541 gccagagatc gacagtacta agatgataat agcgatccct ttaccgacat tatcgaataa
      601 tgcagccaag acatagacga tcatcatgaa cgctaaggcg atcaagacag caaatgacac
      661 gctgtagacc ggattttgga catctactcc tagtaagaaa atattcccta atgtaacgat
      721 caagctttgc gcaatcgcca ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg
      781 tttcgtaaaa ttgtttcggt ctttctttcc taaatagtat tctgtcgtag caacacttga
      841 aagaagaaca gcgccgaccc acagacatag tgcagtataa aacggggtgc tagctgaacc
      901 gttatttgcg attggataaa gcgcattggt ttgtagctca acaggttgtg taaagaagtc
      961 actttctttt gttgcgtcta gtttcaataa tttgatgact tcgcctagat cgacttcttg
     1021 ttcgcctttt cggatcgcgt ctgctgcttt ttggatacca gctttgatat ttggccagtc
     1081 attgttgatc aagtccgcag cagcgttcac agctgtttcg acttctggca ttttttcatt
     1141 tactgttgtc aatgtaccgg tgatttcatt ttttagtgct ggccaatcat tttggataaa
     1201 atcagcggcc aatcctaatt tttgctgaat gatcggtaat tcattgttgt ataggtctgc
     1261 cccttcattg atgccattga cgatggtatc catatttcca ttcagcattg tattggcatc
     1321 atggatttct tgtccaattg caggcatttc tttttgatat ttttctaaaa ttgccactgc
     1381 attcgttaca gttgctttcg ttgagctgag aagcgaatca aagtcgatct ggtccgcttt
     1441 gctcagtgcg ccttgtgcag tcccaatcgt atcgatgact tggtctagta gagaagtgac
     1501 agtggaactg atttgacctg tattgatacc agcaatgatg ccattgatat tattaacaac
     1561 ggcatcaatt tgatctagca attcatcaat agccttcgtg tcttcatttt gaacagctgt
     1621 gttcaaagca gataggcggt tttgcagatt gcttagcaag ggttttaatt gatttagctg
     1681 atcaataatt ggctgtaatt tcgtatttcc agcacttgtt tgcaatttag tcaggaactc
     1741 gaccagttta tcgattgcat tttgttgttg agatattccg tcaataaagc cgttcaatac
     1801 attgctgatg tttttctttt catcatcagt cagcttacca tcttcaatag ctgttcttac
     1861 agtatctatt aacgaacctg tcgttgttgc cacttgtcca attgacttca atgtaacatc
     1921 tacactgttt gtgatgcttg gtatcgcatc tttcagctga gaggcacctg attttgtttg
     1981 gtttgctaga tcacttgcct gattttctaa atttttcaca tctggcaaga tatcttgtac
     2041 ttgttggatg atcgtcaagc cttgttttgc ttcatcgatt ccttggttca ttgtctcttg
     2101 gatcgaagca aaatcgccgt cgatctctgc tagttgctta ccggcatttt ggatctctgg
     2161 gattttttct tgcaaatcaa ggatgatttt tgcttgatct ttcaactcag gcattttgtc
     2221 attcaatgca acgacttttt cacccatctc atctacttta gggatgtaat ccacgaattc
     2281 atttgcttta ttcagttttt ccttgatttc aggtagttga ctttgtaatt cgaggacttg
     2341 ttttgtgtag ccgtcgatcg tgtccaaatt ttcatctgtt gataaaatca tatctttaac
     2401 tttattgata cttactaggt tcgtatcgat gtcgtaacct atctcattga atactttcaa
     2461 taaagtcgaa ctggctgttt tgataaattc atttgtgatt tgcgattgga tcgaacttgc
     2521 tcctttatcg gtgattttcg gtgcaatcgc attgattttt tcatttaccg tatattcgat
     2581 ttttggtttc ttgatatcac cactagtaaa actcagcaga tcttctgaga aatcctttgg
     2641 caagtaaata cctgcgtaat actttccgga acggactccg tcttctaatt gttcttttga
     2701 atcaacgaat tgccatccta attgtttatt tttatgaagc gattcgatga cttgctcccc
     2761 aatggccact tctttacctt gaaactctgc tggcttgtct gcactgtaaa cagcaattgg
     2821 cagctctcca gtatttccat atggatccca taatgctttg atgttaaacc acgcatatag
     2881 cgaagggatg atcatgatcg cgatgatcag aagtgttgca atcgggtttt tgaaaatccg
     2941 tttccagtca agcaaatata atcgaagcgt atttttgatt gactgcatag ttctccccct
     3001 tcttcttttt tcagctgaat tactttacca tgtatttcat acttttttca atcggctttt
     3061 ttcaaaaaag tcgaattttt ggacatatca cggcaattgt ctcattgtaa tctcttacat
     3121 attgagaaat aattaaggat tacagccttt actttatcat gaaataaaag ggaaagataa
     3181 ttttatgcca aagtttttat ctattatacg tatacgcaaa aaagctaaaa cattttttgt
     3241 ctttgcccct acaaaaatgt ttcagctttt ttcgtttttt attcctgtat ttcttatttt
     3301 tcgaatgaca ggatgatttt cccagcagga ggatgtcctt caactagctg atgtgcttgg
     3361 atgacatgct taagtgaagc agacagattc attgaaataa aaacatgaaa ggctcctttt
     3421 tggtaggctt tgattatgcc agcaaaggct tctgcatcta gatattcctt tcttggaaca
     3481 aacgattcat aaaagccttc ctttttttgc cgcaaatcta gatctgggag gtcatttaat
     3541 gcaacatacc tgccgcctgg tttcatgatc tggatgccgg tttctccttt gatacttcct
     3601 ttcgtcgcat cgattacaag gtctgcctga tcagcaaatt gcagacctgg atttgtttta
     3661 tcataagcag caaatgctga agctcctaat ttttttacct tttcctcatt tttactggat
     3721 gcactggtta agattcggat tcccttttca tggagaagct ggatcagact tgacccgacc
     3781 gcacctgatg ctcctagtat cattacagta tctgtcggct ggatttccgt caaatggttg
     3841 attagattat aagccgtaat tcctggcgtg accattccgg ctgcttcttc ccaactcatt
     3901 ttatctggaa tttttgctac ttttgctgat ggcaacacga cttcttctcc gtaagtcccg
     3961 ccgactgcat ggacagctac tcgatcacct acatgaacag ttgtgacatc tgaaccaatc
     4021 tctgtcacaa taccagcgcc atcattacct ggtacataag ggaatttcaa tgttcgtatc
     4081 tctttcattt ctcctaaacg caaggcaaca tcataaggat tcacactgaa tgcttttatt
     4141 tctacacgaa catgtccaga accgacttct ctgggatgag catcgatttc ttcaaaaaca
     4201 tcttgtgctt ctccgtaacc gttaattgca aatcttcgca tggtcaccat ctcccttttt
     4261 ctttaagttt acgtttatgc ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca
     4321 tgattcaaaa caaaatagtt gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag
     4381 cgtaaagatt acgatttacg ctaataaaaa ataattggag gaatttgttg atggctaaaa
     4441 aatcaaagat tgccaaagca aaaaaacagc aagaaacgat tgaacgctat gctgctttac
     4501 gccaggaatt aaaagcgcaa aaagattacg aaggcttgcg taaacttcct ttagatgctc
     4561 gtccagaacg attgaaaaat cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta
     4621 aattcgggat gtcccgtata aaattccgtg aattagcaca taaaggtttg attccaggag
     4681 taaaaaaagc cagctggtaa tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga
     4741 ccactccttg gatcatcaaa aaagatacgt tcgcttcttc tagcagaaat gctggtacta
     4801 gaagcagacg tatctttttt tacgcacagc tggtcctttt ttatatgtaa aggctgcatg
     4861 gttacttcgc tatatttatt tagtaacagg agtcagtgct tcgataccca cactaatttt
     4921 caatgctcgg tcaacttctg ccataatggg tcgatccaaa tggcagacct tttctttcaa
     4981 tcttgattta tcgatcgttc tgatctgctc caacaggata acggagtctc tttctattcc
     5041 tgttccagta gctttgatcc caatgtgagt cggaagctta ggtttagcca tttttgccgt
     5101 tatggccgca acaatgatcg tcgggctaaa atggttacct agattatttt gaataaccaa
     5161 aactgggcgt gttccgcctt gctccgatcc cacgactgga gaaagatcag caaaataaat
     5221 atctcctctt tttaccatag gatcctcctt attcttgata ttctcttggt atccgctgtc
     5281 ctaatccgca agccacttca tagtggatcg tacctaattg atctgctata tcctgcatag
     5341 tgatttcttt gtctgcattt ttgccgataa gcgtcacttt tgttccgaca ggaaattctt
     5401 gaggcaatcg gatcatcagt tgatccatac atacacgccc aatcgtttca cagtagttcc
     5461 cttctaccaa tagagaaaaa ccctgcatct ttcgtggcca gccatctgca taaccgatcg
     5521 gtatcgttcc gatccactct gcttcaggag tgatatatgt ttctccatag ccgattcctt
     5581 cacctgctgg gagttttttg acttgaatca attcagaaac cagctcaagt gctggctgca
     5641 atggataaac ttccggtagt gcgtgcccag agggattaag tccgtacatt gctactccat
     5701 aacgaatcat attgccgatt gtttcatcat gccataaagc cgttgcacta ttgctgacat
     5761 gtacatatcg aggaagttcg ggaagttttt tcagcacttc tataaagcgt tctttttgca
     5821 gattccaata tgtatcatct gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc
     5881 attctaatac acgactttct ttgatgaatg cagctgcctc ttttacttct tcaggtgtac
     5941 aaaatccgat acgacccatc ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta
     6001 gcttactttt ctctaaaaca gcagctgctg cttgcaacca ttcccgtgta cctgcagtta
     6061 ctgataaatc atgagcaaca gctacagtca gtgcgtcgta aggaaccata ttcaagataa
     6121 ggatgggctg agtaaatcca gcttctctca actcgactcc ttcatctaaa tttgaaacac
     6181 aaaagcccga agctccacct gcaactgctg cctcagctgt ttcaatagct ccatgaccgt
     6241 atccgttcgc ttttactact gcaaacaatt cttttccttg cggaagtctt gcaacttcat
     6301 ttgctacatt ttcagttata gcttttttat gtatcactgc tttcgttggg cggtgccaag
     6361 cgacaaccat cgtttttcct tctttctagt cttccaaaat aatttgtgca aaagctgttt
     6421 catttgtatg agtaatactg acccatacat tgccttcatg aggtgaacgc gtcacaacag
     6481 gctgaccatt ttcatttttt aaaatctcga catcttgaaa ggtaactttt ccgatcccag
     6541 ttccccaagc tttagagaaa gcctctttgc aagcgtaacg cccgcctaaa aattctactt
     6601 ggcgatgaaa aggcaaggac tggaatagtt tcatttcatc agaggtcaag attcgtgcaa
     6661 tgaattttgg tttttcttcg
//
LOCUS       DACDHJ010000131         6505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDHJ010000131 DACDHJ010000000
VERSION     DACDHJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.133"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(723..1286)
                     /locus_tag="IV223_001965"
     CDS             complement(723..1286)
                     /locus_tag="IV223_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8712228.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(1301..2344)
                     /locus_tag="IV223_001966"
     CDS             complement(1301..2344)
                     /locus_tag="IV223_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712229.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(2477..2881)
                     /locus_tag="IV223_001967"
     CDS             complement(2477..2881)
                     /locus_tag="IV223_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8712230.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(3114..3749)
                     /locus_tag="IV223_001968"
     CDS             complement(3114..3749)
                     /locus_tag="IV223_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8712231.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(4015..5067)
                     /locus_tag="IV223_001969"
     CDS             complement(4015..5067)
                     /locus_tag="IV223_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8712232.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(5080..6018)
                     /locus_tag="IV223_001970"
     CDS             complement(5080..6018)
                     /locus_tag="IV223_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8712233.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         2074 a         1334 c         1032 g         2065 t
ORIGIN      
        1 attgattgga ccagaaaaaa ttgaagataa ccctttgact ctagcaaaat ggaaggcaat
       61 atcccccgat gtagaaatag tatgaacatt tctaaaataa ttgattgaat aaagtccagc
      121 aataattgct aggagaatgg acaatattat ttttttcttt acttgcatta cttaattata
      181 tactcctttc atggattgct ttatctagta taacttaaat tttaaataaa tcgttattta
      241 tttttactct cacttaaaaa agcactaaaa ttataagaga gggaggtaat ggtctgaaca
      301 ccaagaattg ttccaacatc gaattgctta actaatgagt tcaaagctta attatgtggc
      361 tcttatattt accgagcgtg gattgcgatc tctttagcga aacaaacatc tttcataaaa
      421 aaaacagcca tatccgcttt cttggatatg actatttact actactttac tatctttcat
      481 tcttcttgtc tcacttatga taaggcgctg cgtatcatga gtgaatgata ttaggaaaac
      541 ttcataaaat aaatctttta tacatttcca cttctagatg gtcattcatg ttagaagaat
      601 caaactattt ttttactgtc gtaataatat taaaacacta gaacaaatca attaaattcc
      661 ataaaatata aataatagac ctagcatcat ctggtataaa atggatgcta ggtccgttat
      721 atttactcaa aattgattct acgaatagat cttcttatca tttctatttt agtagtcatg
      781 cgttgttgtt cttctaaagg tggtaatggt ataagtagtt tagataaatt tgtaccattt
      841 acatttggtt gcccagttcc gtaagaaatt ttttctaata acttccaata taatggcgag
      901 tctaagaagc agtctacgta ttctgataat aatttctcaa ctaacctaat tcgaatcaag
      961 taagaagcaa aaacagattc ctcacttatt tccttaacca agaaactttt tcccatagtt
     1021 cctcctgtcc ttgctatcaa aatgtcattt tcttccaatc ttaaatcaac tagtttagaa
     1081 ttactaatat cacagtaagg cacactggac caatttacac gtccctcttg tatatctgtg
     1141 attctcaaaa acttaacatt gccttgtttt ttcgccgaat ctgtatagcc atattgaatc
     1201 ttagtagaaa tacttcctaa ataaacccat agtcgaacat cgctagatga tttttcttgt
     1261 cgttctttat gaacaacagt gatcatcttg agttgtccga ctatttttta gttgcagcaa
     1321 tctttttggg acgcttattt gcatgaagca acgttgctaa gtctagtaat aaaacattgg
     1381 ctaacacagg attggctttc tcgcgatacg ctgcggcgcc actttcaaac aatactgcta
     1441 gacgatcttc tacaacaacg atttgttcca aatacggtgg aaatttgatt cgttttagga
     1501 tccggctagg agagtcccgc tcggacacta agagttccga ggtataagga ccataagaac
     1561 gactggctaa gagataatgg ccataaaaag ccacaccttg aatcttatca gatgtttttc
     1621 tgctcattgt cacggttggt tggattttgt ctttgcggcg aattgctgtt ttcatttcag
     1681 ctatcgtcgg caatagatag aaagtcaact gtccttcacc ttttagtgtg aagttgccag
     1741 ataccagttg atcattgttt tgagtcaaat acgatgcttg tggaatctct gagagatcag
     1801 ttgtcagcca ataagcgatt ggctgtcccg tctcttcaga tgtatctgct aagatatcga
     1861 tcaaacgtaa agcggctact cgtccagtag cagatcctgt gattgtcaac cataaaagtt
     1921 cccgttttgt atcatatact aagcccccta catgtggccg tttaggtaaa atcagcagtt
     1981 tgatcggctg tcctgtttct ctatccagta catgaataat agaatgatgc tgatggtcat
     2041 agcagtaggc agaaataaac acaaaatctt gagaaaaagc caacccttgc ggcgtcatcc
     2101 attgacaaac ctcattttcg ccggtttccg gcaccaccat tcttgtttct gttaatccag
     2161 gaatgttata ttgttttgct tcagcctttt cccaagctgc ttgaacaata ggataccgtt
     2221 ttaacttttt ttcaatatcc tgatgtatcc gttgctgctt ccaatgctta aactttagga
     2281 catagtaaac aagaaggcca atgaccagca acactaaaat cattattgct acccgctgtg
     2341 tcattttttt gcctccttgt tctattcgat caatcgtcca tataccgcta ttaattaccg
     2401 taactacatg tttcttttac tagcgtttct ttataggatc tttcataatc tcatttatag
     2461 taacattaag aatcccttat tctactatag tcacttccac atcaatattg cctcttgttg
     2521 ctttagagta tgggcaaaat gcgtgggctt cttctgcgat tttctctgct tggtccttct
     2581 caagtcctgg caaagaaact tctaaaacga ccccgatttg gtagccccca tcttctaatt
     2641 gaaataaaga tacccgtgct ttgacttcag aatcagactc aagattattt tcttccagca
     2701 tgtgttgaac cgcgccgttg aagcaagatg cgtatccagc cgcaaaaagt tgttccggat
     2761 ttgtcccttc actatggatt cctgggcggt cgatcttcat gtgcatactt ccgtttggtg
     2821 cctcaacttc gccatcacgg cctccacgat tgatcattgc tgtttcgtac atttttttca
     2881 ttgagatcat cctttctttc gtcgttctat attaagtgta acgagtattt tttaatctga
     2941 caaaggtaat gcttgcattc ggcgacatca gttagacaac atcgatgatc agtgatagaa
     3001 gcagtttatc tatacaaaaa aagaagggtt ctatgatatt tggtgtcctt actcgccttg
     3061 agtaacaaca ccaaatatca tagaaccact taatcagctt cacactgctt tttctacttt
     3121 cgtagttgaa aaaaacttaa acaaatcatc ggcaaatagt taaagaaaaa acttaatcgt
     3181 tgaaagaagc ccaattgctt aaagaaggca aactgactaa tcgacggaat ccgtgctaat
     3241 ccgtaaagac ctgcaattag taaactaata actgttaaaa caagataaaa atggctaagc
     3301 gtaccttgac cactttgacg ataaagcaga acaagtaaca atgggaaaag tagaaagcct
     3361 gcatagccta gcccagaacc tatattatgg atccaagtcg atagattaca agagctttca
     3421 ttcgtattga tactgaataa gccagtaaaa atacaatcac ctatcccata taaaccaact
     3481 gttgcaacaa caagtattgc aagtgttttt gatgttccaa caaataggtg atacaacgca
     3541 ggcaacgaaa gaacaaaaaa aagtccagat acaactgacc agaccaagaa agcacgtctg
     3601 actggactat ctacgtcacc aaacacacta attacggtag tcatttgatt cagtttggga
     3661 taaaaaaggc caaggatata cggtgtcgat aaatcactca tcacacctaa taataaaaaa
     3721 taaaaaccat atttttgcaa aaaaatcatt ttcttcccct actttttagt gattattcga
     3781 tacatccaac aatcaaattt tttctcattt taacacatat ttttattcta gttgtctttt
     3841 gaacaatcaa taacataaag gtgagtttct tctacatttt aaaaaatcga atggcactga
     3901 agagttcagt tgtcagcatc aatctcctac tcaaaaaggc aaaaaaacgt aagtcagatg
     3961 ttgttgacat ctcacttacg catccattga ttgaaaattt cttagtaaaa tctactagcc
     4021 taaaaatttg aagacttctt ctcgatcata cagtttaaat actttctcgc ataaaaattg
     4081 agtaattagt gccgcaaaaa cagggatgac taaccaacaa agaacaacaa gaaagccatt
     4141 tagccctgca tctaaagaag ccaacggacc aacaagtccg accacgccga aaccagcaga
     4201 agcaggcgta cctgaaatag aaaatagagc gacaggaata gctgtaatca ctgcactaac
     4261 taaacaagga ataagaatca caggttttct aaatagatta ggcatcatca tcttcatagc
     4321 gcctaaagca atcgcaattg tcacacctga cttattaacc ttccaagagt tcactactaa
     4381 aacaaaagtg gttgcagcta cgcccatcgc agccgcacca gcagaaacac catctagttg
     4441 gattgccatt ccgattgcta ctgttgaaat aggagagata atcaaaaagg caaaggaaca
     4501 accaatcaag atagacatta acagcggttg taaggtagtg aagttattaa tcaatgatcc
     4561 aattgcagaa gtaattgctg ttacaaaagg gaataaataa aaaccaagta aacctgcacc
     4621 tacacctaca acaatcggtg ttaacacaat cgacactgac ccaaaactat ttccaatcag
     4681 taaaatcagc ccgacagcaa tcgatgctgt cagcatcgta ttaataatat cgcctgtacc
     4741 agcaccaacg taaacacccg tttcacccaa atcaggtgtg aatttgacta caccagaacc
     4801 aacaaaagaa gctccggcta caaccatcat tctcatcgga tcaaatccaa attgtaaggc
     4861 aatcaaacca ccaatgatca aaggtgttgc tagttgaaaa ataactgcta actgtgcgat
     4921 cgttaccgca aagggatacg cactaaaata ttttaaaatg gcacctaata ctgcattagg
     4981 aatcaatcca ataatgattc ctgttgctgt accagcgagg actttattca gaaaaatttt
     5041 cggtgatatt ttttgtgaag tttccattca atctctcctt tattttacgt tcaaactatc
     5101 ttcttttgca tggacaagct cagtcaagaa atcacaataa ggcgtcgcaa caccatattt
     5161 cttccctttt ctagaaattg cgccattgat ataatcgatt tctgttaatc gatggttctt
     5221 aatcaagtct tgatacattg aaggataatg taacccaatt gtttccgggt caaaacaaga
     5281 ttcacaatgt gcaatgactt ctgggacatc tagttcaatc ttctctactg ctgctacttt
     5341 ggcaaactca ttgacaatcg tcgccaccat tttatgagca gtcgatgttt ttcctagctc
     5401 agccatatta acgtctaaga ttgtgcatag tccgttcatt gttccgttca cacatgcttt
     5461 gcgataaata gaatagtgaa tattgtcaga aaaatgagca tttaatcctg attcagacaa
     5521 tttgtctgct aatttttttg cagctgcttc ttttccatca cctaaatttt gtaattctac
     5581 tgaaccactt ccaaataatt tgacctgacc aggaccttca agacctgccg tccacatggt
     5641 atttccaata tagatatttt ccattggtac aaatttctca ataatatctt catgcccgat
     5701 accatttaat agacataaga cctctgtatc ttttttgatt aatgattgga tatcctgcag
     5761 cattttttcc agctgcatcg ctttggtaaa tagaataatc agatcaactt gatcttcttt
     5821 ttctacttcg gattgaagaa cgattggtag ttttgcttct acctctttac cattaaaatt
     5881 tgcttgtaat ccatgttctt tgatttgttg gacatgttct gcccagccat cgattaataa
     5941 tacttcgttc ccactttggt gaagcatcaa cccaaagcga cttcccattg cgccagcgcc
     6001 tgcaattgct attttcattg attcttcctc ctttttcttt ctattatact catttgagaa
     6061 tcttttcaca acctcgtttt gttattcttc tcttatttat catgtttata tccaccattc
     6121 aagttatttt atcagcaaat caataaaaac tggaatagaa tcccaatttc atcgtaagag
     6181 aaactagact agcaataaga aaatagaaac attcatagaa aaaatctcac tcctctgatt
     6241 ataaaagagg gggcattaac ctcgtgtata ctaaaacctt ccattcattc gatggatgat
     6301 tttctttttg tgaaaaaata ttcattgtag tacgatgttg cgcaacacag acggatggta
     6361 ttgcttatta tttattaaaa tcgaacaata cacttattta aaaacttctt tcccagattt
     6421 tcttccattt ttacagttat aactagtatt atcatgaaaa atagcctttt attgtctatg
     6481 ggaataataa tttaaaattg gtact
//
LOCUS       DACDHJ010000132         6426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDHJ010000132 DACDHJ010000000
VERSION     DACDHJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.134"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            34..3222
                     /gene="fss3"
                     /locus_tag="IV223_001971"
     CDS             34..3222
                     /gene="fss3"
                     /locus_tag="IV223_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8712234.1"
                     /translation="MLSLAVQAIVGAIAPTITFADEITHPQTVTVELDLAHQYAVEGT
                     FSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKNPLPDMSDKAKLVS
                     VLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIDDYQK
                     KPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVNGNQLIITPNANSK
                     ESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVETEGSLKIKKVDKE
                     SGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTT
                     PMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSLAGNTFAIRKDSPT
                     GEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELKYANQT
                     VAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTLFTAKDGKAVKWSE
                     AFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYSLDETKYPVS
                     IKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGFNDLTFKVSPLEGT
                     NEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFK
                     ENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNRLMLYDLPEEEDSL
                     TSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDVDATKAAQEKDEKA
                     KPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYENKEAFEAGDKPVA
                     KDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFDDVSITHDVLDGSK
                     EAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDTGKYPEGTKFTFAE
                     INYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVPSGSQESSPTVKTF
                     PQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3335..3649
                     /locus_tag="IV223_001972"
     CDS             3335..3649
                     /locus_tag="IV223_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8712235.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3662..4036
                     /locus_tag="IV223_001973"
     CDS             3662..4036
                     /locus_tag="IV223_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8712236.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4046..4381
                     /locus_tag="IV223_001974"
     CDS             4046..4381
                     /locus_tag="IV223_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8712237.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4464..5804
                     /locus_tag="IV223_001975"
     CDS             4464..5804
                     /locus_tag="IV223_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8712238.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5882..>6426
                     /locus_tag="IV223_001976"
     CDS             5882..>6426
                     /locus_tag="IV223_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8712239.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDV"
BASE COUNT         2153 a         1085 c         1435 g         1753 t
ORIGIN      
        1 aaaaaacaaa aaaagaaatt atgtgtatta actattttga gtttggctgt tcaagcaatt
       61 gttggagcaa ttgctcctac gattactttt gctgatgaaa ttacccatcc acaaacggta
      121 accgtagaac tagatttagc acatcaatat gctgttgaag gaacttttag tgatggtcgt
      181 ccaatgtcag aagtcactgt tccgcattat gcagtgtaca acggctttaa acaagatatt
      241 ttctgtattg aaccaggagt cccgatttac aatgagttca ctcctggcta tgagaaaaac
      301 ccgttaccag atatgtccga taaagccaaa ttagtttccg ttttatggaa aaaagtaggt
      361 acagatgcag atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat
      421 acccttcact caataaaacg atcagatggt agtgccgtaa atatcgcagc cattgaggca
      481 aaaataaatc aagccattga cgattatcag aagaaaccaa ccttccataa tagtacggct
      541 aaaacagtat tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag
      601 ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg taatcagttg
      661 ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa gaaatctgct
      721 ggtaccggaa cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg
      781 attgataaac ctaataccta cacaatcaaa attgatgtgg aaacggaggg ttctttaaag
      841 atcaaaaaag tcgataaaga atcgggtgat gttgtaccag gaacggtttt ccatttagac
      901 tttggaaaaa ctttacctgc aaaagacgtg actactgaca aagaaggcat tgctacattg
      961 gatgagattc ctcatggtac aaaagtaacc attaccgaaa aatcagtacc ggcgccttat
     1021 acgattgata ccacccccat gactaccacc attaaagcag gcgaaaccat ttacgtgact
     1081 tcaaaaaacg cacgggaaaa agggcaaatc attctggata aaagcggagt tgaaacaggg
     1141 agtgatcttt ggaatgacaa ttattcctta gctggtaata cgtttgccat tcgcaaagac
     1201 agtccaaccg gtgaaatcgt tcaagaaatg accacggatg aaaacggtca tgcggaaaca
     1261 ccaaaagaga ttgctaatgc gttggaattg ggaacctatt atgtgaccga aactaaagct
     1321 agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta tgccaatcaa
     1381 accgtagccc ctgttaccag taacgtaaaa gggcaaaatc aagaagtcac tggcgaaacc
     1441 actttgacga aagaagacaa agatactggg gataaagcac aaggtaaggc agtctttgag
     1501 ggtgctgaat acaccttgtt tactgctaag gatggaaaag ctgttaaatg gagtgaggct
     1561 tttaaaccag agttagcgaa gggaacgaaa ggttctgatg aaacggtcac tttgacttta
     1621 gacgaaaaga accaagttgc cgttaaacat ttagctatca acgagtatta ttggcaagaa
     1681 acaaaagcgc ccgaaggata tagccttgat gaaacaaagt atcctgtatc catcaaaaag
     1741 gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa agaacaagtg
     1801 attcgctttg gctttgattt ctttaaattt gctggatcag ctgcgggcac taccgaaact
     1861 ggcttcaatg atctgacctt taaagtgtcg ccattggaag ggaccaatga aatcacaggt
     1921 gccgaagatg aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag
     1981 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt
     2041 caaaagatta cgccattaga aatccgttct acttttaagg aaaacaaaga agactttgtg
     2101 aagagtgagt atgtctttac cattaccgaa caagaccaaa aacaaccaat taaaacggtg
     2161 accgttcctt acgagaagct gacgaacaaa gcattttctg ttagtttgaa ccgtttaatg
     2221 ctctatgatt tgcctgagga agaagatagt ttaacttctc ttgcgacttg gaaagacgga
     2281 aacaaagaat taacttctct ggattctacc gagctagttg ataaattgag ttacaatcta
     2341 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga tgcgacaaaa
     2401 gctgcccaag aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac aagcgcaacg
     2461 ttggcgaaca aagagaaaac aggaacttgg aaaattcagc ataaattaac cgccgaacaa
     2521 gttttaaata aaactattgt cttattcaac tatgtgtatg aaaataagga agcttttgaa
     2581 gcaggcgata agccagtagc gaaggatgtt agcttgaata accaagccca aactgtcagt
     2641 tgtacagtgg aacaccatgt ttctatccaa acaaaggccc acctagaaaa cggttctcag
     2701 acctttactc atggtgatgt ggtggatatg tttgatgatg tgtcgattac tcatgatgta
     2761 ctggatggct caaaagaagc tttcgaaaca attctgtatg ctttattacc ggatggtaca
     2821 aacaaagaaa tttggaaatc tggcaagatt gattatgaag taaatgataa agaattcacc
     2881 aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac caagtttact
     2941 tttgcggaaa tcaattacga taaagatggg aatatcaatg gaaaacacaa tgaagatttg
     3001 aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa ccatactaag tacgccgaaa
     3061 caaccggaaa caccaactgt tccaagtggc tctcaagaat ctagtcccac agtgaagacc
     3121 ttccctcaaa ccggggagaa aaattccaat gttttactgt tcattggttt tactttgatt
     3181 tttgcgacgg cgggctatta tttctggaat cgccggaact aaggtgatga gatgatacaa
     3241 gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc gctatttttg
     3301 tatccaaaac taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg tcgtgccaga
     3361 tatggctgaa acctttggaa agttaagtta tgccggtgaa ggagaaatta taacagaagg
     3421 atatggacgg aatactacgg tgattggtcg cagttatcat ttgtattcca gtaagcaacg
     3481 ggccgatgat attgaggtgg tagtagctgc ggaagccggt gaaaaggatt ttgatcaaga
     3541 ccaaccgcta aaagccgtga atccccattt ggttgccaaa ggctatgaga ttgagaatcg
     3601 tgggtttact gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa
     3661 gatgagatta acagaaggta ttgtcgtaga ttcaggttta acatttggga agttgcgttt
     3721 ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacaattg gtaatgaggt
     3781 aaaagaacga acctataatt taaaatcctc tgcacaaggg cgaatgattc aagttagtat
     3841 cccagctaat gtacccttac gcgagtttgc ttatgatgca gaggtagagt tggtcaatcc
     3901 aatcgtggat accgttgcca attatgtttt tcgagaagga actaccgtta attggttcat
     3961 taaggcggat gatttagtct tgaagcggca gccgaatcaa ggaaatccta cgaaccaaaa
     4021 tgaaagaaag aaataggtga agattatgga aattaatgtt gaacatattt tagattgtct
     4081 cgatcaatat ggaaaaggcg agctgactga ggaacaactc gttaaagcat taacctctga
     4141 tgaaaaaatg tttctgatta tacatcaagg tttacttgga gaaaatagcg acagtgaaga
     4201 agactctgat gtactagact ggttaggaga agaagagtct ttctttatag ttgtcgaagt
     4261 aaatgaaaac ttatgtcggc aggcagaatc cattttggaa gaaattggtg tggagatgcc
     4321 ggatgctatt gaaggctttt taaatcaatt ggttgaaacg aaacaactcc cctcctcgta
     4381 gcagtaaatg actagcataa ctttttgaaa ttgcctaatg aaatcctatt gggctttttc
     4441 gattggaggt ggaaaccatc aaaatgtaca aaggacatcg catacgcgca ggagatcagc
     4501 acttggttta tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg
     4561 tcttttattt tcaagagttt agacaatttg atatttctaa gctatcactt tctactattg
     4621 aaatcgtttg gtctataaaa gatttagtat gtctgcttgg aagtctcgct ttttcaggag
     4681 ctatgatatt gctttatatt cacttttttc ttgatcattg gcgaagcttg tggcatcgac
     4741 aaaagctagc tcggatgatt ctagagaacc attggtatga agtgaaacaa actcaaagtg
     4801 aaggcttttt caaagatttg aatagtagcc gaaccagaga gactatcagt tacttcccca
     4861 aaatctatta tcgaatgaaa gatggattac tgtctattcg cgttcaaatt tcacttggga
     4921 aataccaaga tcagctttta aaattggaaa agaagttaga aagcggactg tattgtgagt
     4981 tagtagaaaa ggaactcaaa gactcctatg tggagtatac tttactgtat gatatgatcg
     5041 ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg gactctgcga ttgatgaaaa
     5101 atcaagtatg ggcttatgat tctttacccc atatgttaat cgctggtggc acgggtggag
     5161 ggaagaccta tttcctcctc accattatcg aagcactatt gaagtcagat gcagaattgt
     5221 ttatcctcga tcccaaaaat gcagatttag ctgatttagg tacggtgatg cctcatgttt
     5281 attcacaaaa ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt atgatggctc
     5341 gtagcaaggc aatgaaagaa atgtctaact acaaaacagg agagaattat gcctatcttg
     5401 gacttccacc caacttttta atctttgatg aatacgtggc ttatatggag atgttaacga
     5461 cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg ggtcgtcaat
     5521 ctggtttctt tctgattttg gcttgccaaa ggccggatgc gaaatattta ggagacggaa
     5581 tccgcgatca attcaacttt cgcgtggctt tgggtcgtat gagtgaactc ggatattcca
     5641 tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca catcaaaggt cgaggttatg
     5701 tggatacagg tggcagtgtg attagtgagt tttatacgcc acttgtacca aaagggtatg
     5761 atttcttaga ttcaattagt aaattagaaa agatgaatga ttgagtgatc ttcaatactg
     5821 actttatact tcaagtaacg tataatactt ttatatagta ggaagaaagg ggataattaa
     5881 tatggaaagt aatgattctt atttagagta tgcaaaagca aataaggata agtttatttc
     5941 ttatattatc aaagggaaat cccccgaagt tggacgagac gcagttttta tggcagggag
     6001 tcctggggct ggaaaatcag aggttgccat tggattagct gagaattatg ataaccatgt
     6061 gattattgat gcggattact ttcgcaccca gtttcctgat tataatggaa aaaactctag
     6121 tctctttcaa aaagcatctt cttggttggt agagcaggca tttagatatg tcttagatca
     6181 tggctattct tttattctgg atgcaacttt tgcaatatta agtgctgaaa aaaatatctc
     6241 gcgggcgttg aaaaaacatt ttcgagtgac gattttctat gtttatcaag accctaaaat
     6301 cgcttggaaa tttacacaag aacgtgaaat tgcagaggga cgtcatgtgc cgaaaaaggc
     6361 atttattaac gcttttttca aatcaagaga gaacattgca aaagtaaaga gtaaacatcc
     6421 agatgt
//
LOCUS       DACDHJ010000133         6359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDHJ010000133 DACDHJ010000000
VERSION     DACDHJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.135"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..1645
                     /locus_tag="IV223_001977"
     CDS             50..1645
                     /locus_tag="IV223_001977"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8712240.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            1635..2852
                     /locus_tag="IV223_001978"
     CDS             1635..2852
                     /locus_tag="IV223_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8712241.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2865..6014
                     /locus_tag="IV223_001979"
     CDS             2865..6014
                     /locus_tag="IV223_001979"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8712242.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2229 a         1052 c         1314 g         1764 t
ORIGIN      
        1 aataagaggg tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga
       61 attaaatcaa aaattattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga
      121 atataaaaat tatttattgg gactgatttt ctataaatat ttatccgatc gattgttgga
      181 acaagtggtt cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac
      241 aatgttgtat cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgccacaat
      301 cgttgatatt ttgggatatg caattgcacc agagtatctt ttcaacgtct tagcggatca
      361 agcaaaacaa gcaacgttcc aattgaacga tctaaacaaa gcctttgtgc aattggcatc
      421 tacctacaat caatttaatg ggttatttga tgatgtggat ctccaatcaa aaaaactagg
      481 aacagatgag caacaacgaa atgtgacgat taccgaagtc atcaagaaat tgaatgatgt
      541 agatgtttta gaacacgatg gggacgtgat cggggatgcg tatgagtttc tgatcagtca
      601 atttgcttca gaagcaggga agaaagccgg agaattttac acacctcata tggtttctga
      661 tatgatggcg caaatcgtaa cgctcgatca aaaggaacga cgattcttta gtgtttttga
      721 tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt aactacttaa cacacccaga
      781 taacgttaaa tatcatgggc aagaactaaa tacaacgaca tacaatctag caaaaatgaa
      841 cttgatttta catggtgtcg acgccgaaga aatgaatctc cgcaatgggg atacgttgaa
      901 taaggactgg ccaacagatg aaccttacac atttgatgca gtagtaatga acccacctta
      961 ttcggctaat tggtcagcag atacgacttt cttagatgat tctcggttta atcgttatgg
     1021 gaaattagcg ccaaaatcca aagcagattt tgcgtttctt cttcatgggt tctatcattt
     1081 gaaggaaaca ggtaccatgg ccatcgtctt accacacgga gttttattcc gtggcgctgc
     1141 agaaggggtc atccgtcaaa aattactaga agatggcagt atttatgctg tgatcggtat
     1201 gcctgcgaac ttgttctttg ggacatcgat tccaacgacg gtgattgtgt tgaagaagaa
     1261 tcgtcaaact cgagatgtat tattcattga tgctagtcgt gaatttgtta aagggaaaaa
     1321 tcaaaataaa ctatcggaag aaaacattca aaaaatcctc gaaacctacg ctgaaagaaa
     1381 agatgtcgag aaatacgctc atttggcaac ctttgatgaa atcaaagaaa atgactataa
     1441 cttaaatatc ccacgctatg tagatacttt cgaagaagaa gaaccaattg atatggttca
     1501 tgtcggaaat gacatcaaga agatacgaca agaacagcaa gtgctagaaa aaaaactttt
     1561 agaagctatc tcttccctgc aaacaacacc tgaaaatgaa gcgtggttac aaggtgcgct
     1621 ggaggtgttc aaacatgagc aatgatacac aaccagaaat ccgtttccca ggatttacag
     1681 atgattggga acagaggaag ttgggggact tcgttgtaga ttatgttgaa aagacaagtg
     1741 tacaaaatca atttcctatg ctaacttcat ctcaacaaaa agggatagtt ttgcaagaag
     1801 actattttgc aaatcggcaa gtaactacag aaaataatat tggttacttt gtactaccta
     1861 gaggatattt cacgttccgt agtcgaagtg ataatgatgt gtttgtgttt aaccgtaatg
     1921 acattattga tagagggatt ataagctatt tttatcctgt atttacttta aaatctgctg
     1981 attccgactt cttcttaaga cgtattaaca atggaatcca acgacaatta tctattcaag
     2041 cagagggaac aggtcaacat gttctgtctc taaaaaagtt taaaaatata gtagcaatgt
     2101 ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt caaacaactt gacgacacga
     2161 tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac gaaaaaagga ttcttacaaa
     2221 aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg gtttcctggt tttacggaag
     2281 attgggaaga gcgtaagttg ggagaattag gtaaaactca atcgggaatt ggatttcctg
     2341 atgccgagca aggtggttct gaaggtactc cattttttaa agtttccgac atgaataaca
     2401 ttggtaatga gtatgaaatg agaaatgcaa atcattatgt atctaatgag caaattgagc
     2461 gtaaaaaatg gaagccaata aaagatgtgc cagcgattat atttgctaaa gttggcgcag
     2521 caataatgct taatagaaaa cgtttggtca caagcccttt cttaatagat aacaatacca
     2581 tggcttattt atttaataat acttgggata tctattttgg taaaatattg tttgaaacta
     2641 taaatttgcc aagatactcc caagtagggg cattgccttc ttataacagt tctgatattg
     2701 agaatatcag tgttaaggtt cctgtaaagg atgaacaaca aaaaatcggt actttcttca
     2761 aacagctaga tgatacgatc gctcttcatc aacgtaagtt agatttacta aaagaaacga
     2821 aaaaaagctt cttacaaaag atgtttgtct aggagggaag aagaatgaaa attagtcacc
     2881 gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact tggagaaggg cataatcagt
     2941 ggacgtatcg tccggatcta aaatcagaag aagacctttg ggtcaattta cgccaaaaaa
     3001 ttatctcaaa taatcaagca gaactgaatg attttccttt gaccgataaa gaatttgaaa
     3061 cgatcaaaac ggaattactg ctgcgaacga aaaccccatt tgatgctgcg aaatggttaa
     3121 aaggtgaaaa tgggatggct cggatcacga ttgaacggga agattcgcaa ttgggttctg
     3181 tctcacttat cctttattcc aatcaagaca ttggtggagg aatctctact tatgaagtgg
     3241 tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg ggatcgacgt tttgacgtca
     3301 ccttattaat caatggatta ccgattgtcc aaatcgagct gaaacaagta actgcaaaag
     3361 atggtgttta tcaggcattt aaccaaatca aaaaatatgc agaagaaggt atgtttcgga
     3421 acaatatctt ttcgacctta cagttgtttg tggtttccaa tgaacaaacc actcggtatt
     3481 ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt atttagttgg cgaacgaaag
     3541 acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca agtgttaaat atcccggatg
     3601 ctcatcgttt gattgcggat tatacaatcg tcagtgaaga tcaagacaat aaaaccctca
     3661 tggtgttaca tccttatcaa gtacatgcga ttcaagcctt gtttattgcc gcaaataaac
     3721 atcaatcagg gtatgtgtgg catgcgactg gttcaggaaa gacgttgact agttttgtat
     3781 ctactaagct tttagcacga aaatcgggga tcgatcgaac gatcatgtta gtggaccgca
     3841 aagatttaga taatcaaacc accacagagt ttacaaaatt tgcatcggaa tttaatactg
     3901 ggatttcttc cgggaacgcc aaagcgaata gcttgatcgt tggaacggga agtgccaaag
     3961 aattaagtga aaccctgttg gcagatgcca atgcaaatgt tgtaatcatt acaacccgtc
     4021 aaaaactaga tgcggcgttg aaatatgcaa agaaacaaga agagaaaaaa ggaaccaatc
     4081 gcttccaaaa attaatgggc caacatatcg tgtttgtagt agatgagtgt catcgagcgt
     4141 taagtgctga aaatatggaa gaaatcaaaa aaatgtttcc gaaatccact tggtttggct
     4201 ttacaggtac accgattttt cctgagaatc ggaaacaagc caaagggcaa ttggcacgta
     4261 caacccatga tcagtatgga gaggtcttgc atacctatac cattaagaat gcgctagaag
     4321 atggctctgt cttaggattt caagtagagc atgaaaacac cgtggagccg acttcactag
     4381 agaataaaat ctatcggaaa ttaaaagaag tagaaacata tgcagagtat tcatcagaac
     4441 agatcaatcg tatgattgat caaatggaac ctgtcaaaaa agaaagttat ttggacccag
     4501 ctgtttttga agcagatgaa catatccaaa aagtcattca taaaatgttc cgtccagata
     4561 atgcctatac aaaatttgat tttcgaaatg gcaggccaac gaaatctgca atcttaacca
     4621 cgagttccat tgacatggcc aaaaagtatt atcgggccat caaagagatg acgaaagagc
     4681 ctgattggtt aacaaaagag tttcccaatc agctcattcg tgaaggacga acgatggagg
     4741 atcctgattt tccacgaatc gctattacct attcattaga agaaaatagc cgagattcaa
     4801 gtgcccaaca agaagaaatg caaaaaatta ttgaggaata taatcagtat tatgatactt
     4861 cctggtcatt agcagacatt gagcgttata acggagacat caataatcgt ttggcacgaa
     4921 aaagagcgga attcaaacaa tttgggaaac aaatcgattt agcgattgta gtggatcgtc
     4981 tattaacagg ttttgacgcc cctacgattc aaaccctatt tgtggatcgt aatctagagt
     5041 atgcaggatt gatccaagct ttttcacgta cgaatcgtac gtatcctgat aagacaaaag
     5101 gattgattgt gacgtttcgt aagccagcga caatggagaa gaatgtcgag gatgcgacaa
     5161 aactgtattc aaaagcaaaa gaagaatcag gtttgattta tccaagttat caggaatcca
     5221 aaaaaagatt caaacaagca tatggtaagt taaaagaggt ttcatccatg gaatccattg
     5281 atgaacatac gccacttgaa atccgtatcg agtacgtcaa agcatttcaa gaattgaatc
     5341 gtgcttatga atcgttagtg acttatgacg attacaatga tgacatggtg aaatctccaa
     5401 ctttgaacaa tcaaattcaa cttttggaag aacaaattgg cgtgtacgag accgtgaagg
     5461 gttcattaat agaggaagaa ccagaaaaag aaggagaaga tttttcaacg atcgaatttt
     5521 atagtgaaaa taccactaag ttgtatgata ttgattcaac ttatattgat caattactag
     5581 gaacttatgc tgcgaatagt tcagatgcac gagaagaaat tgaaaaagct ttggctaaat
     5641 tgaataaaac agaaggagtc aaagaagttt accgacaaat tttaaatgca tttgatgtgg
     5701 ggactttaga tagtaatgaa gatatctttg tcattaaacg tcgctatttt acacaagcga
     5761 gtgatgattt gattcatgtc ttttccaatg agtggtttgt ttctgagaaa gaattacatg
     5821 cttctaatct gcaatatcta ccaggtgaag atccaattcc taatatgaaa gcaattatca
     5881 acagtaagga ttacgaagga tataaagcca aacatccaga agcgaagcca tttaaatacc
     5941 cacaagaaat gaagcgagca tggcgtaaaa tgttagatga tgagttgatt ccacttgaga
     6001 atgaattgag gtaaacgaga agagttaaga aaattgtacg attcaaagat ggtagtcaag
     6061 tggaagtgta gaaaataggg atagatgtct tttatttgtt cacattttat aacgaagtat
     6121 ttaagacagc tattgaataa tgtttcctcc acctttttca ttcatttacc ccaccccgtt
     6181 gttatatgac cgtgttttga gaggaaccaa taaaaagcat aggctaacta ggggttcctt
     6241 tttcttttgt ttttttggaa tccgcttaag tgaaatgtat ctgaaatgaa acggatataa
     6301 agtgattttt tgctataatg acaaggtttt agctgtttca gataccatgt aacccaaaa
//
LOCUS       DACDHJ010000134         6345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDHJ010000134 DACDHJ010000000
VERSION     DACDHJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.136"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..879
                     /locus_tag="IV223_001980"
     CDS             22..879
                     /locus_tag="IV223_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8712243.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            872..1774
                     /locus_tag="IV223_001981"
     CDS             872..1774
                     /locus_tag="IV223_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8712244.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1807..2157
                     /locus_tag="IV223_001982"
     CDS             1807..2157
                     /locus_tag="IV223_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8712245.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2191..3129
                     /locus_tag="IV223_001983"
     CDS             2191..3129
                     /locus_tag="IV223_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8712246.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3207..4100
                     /locus_tag="IV223_001984"
     CDS             3207..4100
                     /locus_tag="IV223_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8712247.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4138..4701
                     /gene="amaP"
                     /locus_tag="IV223_001985"
     CDS             4138..4701
                     /gene="amaP"
                     /locus_tag="IV223_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8712248.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4715..4903
                     /locus_tag="IV223_001986"
     CDS             4715..4903
                     /locus_tag="IV223_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8712249.1"
                     /translation="MKELISTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            4916..5818
                     /locus_tag="IV223_001987"
     CDS             4916..5818
                     /locus_tag="IV223_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8712250.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKNGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5850..6092
                     /locus_tag="IV223_001988"
     CDS             5850..6092
                     /locus_tag="IV223_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8712251.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT         2287 a         1097 c         1283 g         1678 t
ORIGIN      
        1 ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt ggtaactgct
       61 tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt agaaaattgc
      121 aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga atatagtaaa
      181 gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca attcctgaca
      241 gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac taccgcttac
      301 ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt cctttactta
      361 gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc gttaaatgga
      421 aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag attaggaatc
      481 gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga tcaatggaat
      541 aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga tcgaatcatc
      601 tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca acttcatcca
      661 aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat
      721 gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac aggacatgat
      781 aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa aaaattagca
      841 cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag cagtagtcgc
      901 aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag tattagataa
      961 ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg attttgaaag
     1021 taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg tggaattctt
     1081 gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc
     1141 ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac gtatcttata
     1201 tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg
     1261 tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg aaaaaatgaa
     1321 aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa
     1381 atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata atccagaaaa
     1441 attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt ggttaccact
     1501 tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac atccaacagc
     1561 agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc atttttcagg
     1621 acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt acgaaaaata
     1681 tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa aatatgcaaa
     1741 agttcaacca aaaacagatg tagttgttaa ataatagaag acaacaactg aagaggggga
     1801 gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga aaaggttggg
     1861 gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct aagggacaaa
     1921 ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga atttggagaa
     1981 tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt agagcaaaat
     2041 tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt ttcattagaa
     2101 aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt cagctaatag
     2161 tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga tgaagaaaag
     2221 aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag tgcaaagccc
     2281 gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt cgtaggaacc
     2341 gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact agagcatcca
     2401 ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa aaaattaacg
     2461 ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag aaaaaccgaa
     2521 tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga aaagatcatc
     2581 acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga gatgaaaaag
     2641 cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga actgtataaa
     2701 caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga tcaacgggaa
     2761 acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga tgaacagcta
     2821 ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat ctatcgagat
     2881 ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct aaatcatatc
     2941 atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt cgcagggatt
     3001 tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt tatactcgta
     3061 gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg gaaagattat
     3121 acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt tggcaataca
     3181 atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac ccgcgaaact
     3241 tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta cagcagaata
     3301 tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa ctagaaaacc
     3361 gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg attgcatttg
     3421 ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa gatgcagagg
     3481 aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt cttactacct tttgatcttc
     3541 gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt ggcgggctag
     3601 atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct gagttaccac
     3661 ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct ttagtgaaag
     3721 aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct gttcaagcat
     3781 tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc gctaacttta
     3841 cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt gtagcacctg
     3901 gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg atccctgaat
     3961 ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca ccagtttatg
     4021 ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga gtaactggtg
     4081 gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg
     4141 aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc tgtgcttttg
     4201 tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa gtttcgattc
     4261 ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct
     4321 attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat cggaaacttt
     4381 ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga aggaatggtt
     4441 cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt caaagcaacg
     4501 aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc actcattggt
     4561 aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt gggaacaaaa
     4621 gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa acgaagcaat
     4681 gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactaattt cgacatacaa
     4741 gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga tcaccgtggg
     4801 ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta
     4861 tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa catccatgaa
     4921 taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc
     4981 agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc
     5041 agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag aagagaagaa
     5101 acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta aagttccaga
     5161 acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc cttctgacca
     5221 tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa aacttggttc
     5281 tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa tgcgcggtga
     5341 gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga
     5401 cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac
     5461 agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag cagttgatat
     5521 ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt
     5581 aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca acgctaacgt
     5641 agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc aagacaaagt
     5701 aactgacgca gcgaaaaaaa acggtgaatt tgcttcgaat caaacagata aagtaaaatc
     5761 agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg ttcagtaata
     5821 aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt atcgtcggag
     5881 gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg gggattattg
     5941 caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta ggttcttggg
     6001 gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc attttagtac
     6061 taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa
     6121 agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat
     6181 ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg gaataagagg
     6241 ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca attagatttt
     6301 taaaagtttt tccttaaatg tgtctttatt gatacagaga agctt
//
LOCUS       DACDHJ010000135         6261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDHJ010000135 DACDHJ010000000
VERSION     DACDHJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.137"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2452
                     /locus_tag="IV223_001989"
     CDS             <1..2452
                     /locus_tag="IV223_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8712252.1"
                     /translation="QKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQ
                     VERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEV
                     DIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEE
                     NSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPL
                     RKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQ
                     EYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEI
                     EIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEA
                     MIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASI
                     VLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIK
                     LASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLT
                     SSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKL
                     FEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEH
                     IQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSI
                     QKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQV
                     LPTYEILLPSSLKSYFRL"
     gene            2634..2873
                     /locus_tag="IV223_001990"
     CDS             2634..2873
                     /locus_tag="IV223_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712253.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2889..4262
                     /locus_tag="IV223_001991"
     CDS             2889..4262
                     /locus_tag="IV223_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8712254.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4266..5900
                     /locus_tag="IV223_001992"
     CDS             4266..5900
                     /locus_tag="IV223_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8712255.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6063..>6261
                     /locus_tag="IV223_001993"
     CDS             6063..>6261
                     /locus_tag="IV223_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712256.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2186 a         1025 c         1274 g         1776 t
ORIGIN      
        1 acaaaaaaag cattctgtga gagacaatga tattgaaaaa attcggtttg tatttattga
       61 tattgaccct aaaaggaaag agggaagtgc aacagatagc gaaaagaaaa aggcagaaaa
      121 tgttatggaa caagtagaac gttatttgaa agaaaagaga atagaatcat ttgtaaaagt
      181 tgatagtggg aatggctacc atatttttct tcccataaat gaacaaccaa ataatcacga
      241 gacgattctt actattcaga attttcttca attacttcac cgaagatttg gagtagaaga
      301 tgaagttgat attgatactt ctgtttataa tccttcaaga ttatgcaaat tagttggtac
      361 accagcggtc aaaggaaagt ccacaaaaga gcgtccgcat cgtttatcta gtttcctttc
      421 tattcccgat aacatagata gaagtaattg ttttaaaatt cttgaacaga tagttgaaga
      481 aaattccatg atgtcaacct ctagagtaaa aagccatcca tcttttagag aagtgttacc
      541 ttttattcaa gcagactata aacaatggtt agctcactat cctgaattaa cttataccat
      601 aaaagagggc gatcataaag gagttacctt acttatattt gacgaatgtc cattaagaaa
      661 acacacgaat aattctaatg gttcttgttt gtctgttacg gctgataata agattcgatt
      721 tcattgtctg catgcttcgg ataaaaataa agatattcaa gactttgtaa aaaaatatcc
      781 gttgccaaaa cctgtaaatc cttctgtaga atcattagaa tccaatcaag aatattattt
      841 tgaccagttt aaactgaatc aagaaggagt atttttggtg aaagaaggtc aagaagaaaa
      901 agttgcttgt ccgatgttta ttaagcaaat acgcaaaaat atcgaacaaa aagtcattac
      961 acttgttctt gcctattatg tcgatcattg ttgggatgaa atagaaatag aaggtggatg
     1021 tttacaaact aataatttta aacaattagc aaaacatggc ttaatcatta aagctagaag
     1081 agaagctgaa gtcgttgact ttttacagat tcaacgggag aatgctccaa tcttttttga
     1141 acatgaatct cttggctgga aaaaagaaaa tgaagcaatg atttttcagt tacaagattc
     1201 ttttggacgt gaaattcctt ctgtattaac taaaaactca tgctaccaat taacaactaa
     1261 tggtacagaa gcggaatttg acgcattggt tcataagtat gttttaggaa cgaacttaga
     1321 acttgcttgg tcgattggta ttgcttctat tgtgttaggc tatttaaacg ttacgaaggc
     1381 taatactttt ttgagtttac taattaacct acagggcaag agcacaacgg gtaaaaccac
     1441 tacgttacac tttattgcct ctttatttgg aaatccaact acggttctta gaaatatgaa
     1501 tgctaccaat aaagccatta taaagctagc atcaaacaat cagggaggtg ttccactaat
     1561 actagatgag ttaggagcag caactcaaaa taactttagc tcgttgatct atcaattagc
     1621 ttcaggagaa gaaaggctaa gattagattc taattgtcag cttatgccac aagagacttt
     1681 ttctgttttg acccttacct cttctgaaga gcgtttggaa aattatttca atgagaaatt
     1741 aactggtttg aaagttcgat atatcgaatt ttctgatatc aagtttacaa aggatgcgaa
     1801 tcacgcagag aaagttaaaa gtctttcttc taatactttt ggttggggta taaagttatt
     1861 tcttttgaaa ctctttgaac aagggattac aaaaattgaa gcaacttttg tcgaagctca
     1921 aaaaaaacta tgggctgttt tacctgaaca ttctttgaag caacgcattg gtaataatct
     1981 agctttgatt tatagtggag ccctcctaat tcaagatata ctgggctatc ctttacgatt
     2041 agagcatata cagacggtgc ttattaattc gtacgaacag ttaattgtag atactagttt
     2101 aggaagtgag caaacggtat ttgaccgagt gaaaacattg ttggtgatga atagccataa
     2161 atttttaaca aacagtgaca acaaacatac gcccagtacg atttggggaa aagtatcaat
     2221 tcaaaaaaat ggggatatta aggtaaatat ttttcctatc ccgtttgagg acttgttaag
     2281 gcgagaattt caagtaaagg atctaaccta tctttttagg caattgcttg attcgggtaa
     2341 tatgtgtgca gaaaaaggga gaagaacgaa acgtattcac atgaatcgtc aagttcttcc
     2401 aacatacgag attctattac cttcttcact caaatcgtat tttcgattgt aatttagaat
     2461 aaaccaaagt atgaaagtga ggcaccatgt tatggaaata tttcttattg atttagcaaa
     2521 acaactgatt acttatctgt atgatagcga gcaaacccaa acggaagtaa atgaagcttg
     2581 atctatgctt catttacttc attcaatcaa acatgaaaga aaggaaaatt ctaatgacta
     2641 aaaataaaat gacactaaaa gcagaggttc ttctgtatat tcaagaacat ttttcaaatc
     2701 aagccttttt tacaaaacca atttacttgg atttcgaaat aagaggggta tctgctggat
     2761 ctatcggggg tactcttcaa gcgttgaaga acgaaggata tttagaaaat cactttgtgc
     2821 aacgttcgtt caatagaaga gtcgtgaaaa aatggtattt agttcattca tagagagggg
     2881 aatcaagcat gcgtgtggct tgttatgatc gaacgtctgt ttatgaagaa gcgaaaatta
     2941 gtaatgacgc tcaatatgat gcagtgaaag agtatgtttt agagaaatat gaacaaattc
     3001 cccaacgatt ttcagacaaa ggagaatcag gggaaaaagc ggagcgtcca gcttttcgac
     3061 agcttagagc ttgggtttgt gcgtcagaag aagagggggt gatctatgtt tggcgttatg
     3121 atcgattggc acgtcaagcc aaaatagcct tagaatttat tgagttgtgc caacaacatc
     3181 aggttcaagt gtattcgatt tctgaacctc ttcctgcttc tttctcacac gcgtcaacga
     3241 ataaactatt tgtgcaatta ttgttcactt ttgcagaaca acaacgaaac gtcattattg
     3301 agaatgttcg tgccggactt tcctataaaa agcaacaacg gcaatatttg tcttctgcgg
     3361 taccatatgg gtatcgttta gtgaatggga aaatccaaca agaagtacac gaagcaagga
     3421 ttgttcgcta tatttttcaa ctttatttga ccaaaaaata tggctacaaa aagctttgtc
     3481 aacgattgac gcaacaaaag cttttcttcc gcgaacgtcc ttttcagccc tatcatattt
     3541 atagtatcct aaaaaatcca ttgtactatg gcgaaatcaa aggaggatct ttggggaagt
     3601 atctaggaac ctttgaaccg attctctcga aagcaacctt tttacaagcc caagagattc
     3661 gccaatcaag gtgtacagct aaaaaagata cgtatctgta cttattacgt caaaaaattc
     3721 ggtgtccctt ctgtggtcgt catctgagtt cgaagtatca atggaataca aagaagacga
     3781 agacgcttca ttattatcat tgtaccgacc gaagctgcca aggaattttt cttcgggcag
     3841 aaagggttga gtcagaagta ttggaaaaga tacaggcttt ttttcaatac gaaggcgtct
     3901 atcaagcctt gattgcccaa ttaacgcaac aattagccga aaagcgaaaa gatgaaaaac
     3961 gcaaagtaat ccaacaaaag aaacaagaag cacaatggat taaggcattt gaagagggca
     4021 attgttcact ggaagaactc aagcaacaac tgcaagctct tcaaaagccg atgtctactt
     4081 cttcgtttct ttcactccaa cagtgtgaag caaaagtgaa agaatggctt caactaaaag
     4141 acctctctag tcgacaatta ttatgtgact atgtttgtcg gattgaggta acaaaggata
     4201 aaacagtttg tgggatttac ttccgacaac tgacacaaaa tattttagaa aggaacgaat
     4261 gatctatgga aatgatacaa accaaacgag tcgcattgta tattcgcgtg agtacacaag
     4321 aacaagcttt agaaggacat agtattcaag cacaagaaca aaatggacga cagtttgccg
     4381 aacggatggg ctatgaagtg gtagaagttt atgccgatga aggaatcagt gggaaatcca
     4441 caaaaaatcg gttcgcctat caacgaatga tgaacgatgc tcggaaccat cgttttgatc
     4501 tactagtgat ttggaaattg acccgtctcg gacgcaatat gttagatgtc ttacaaacag
     4561 tcgaggaatt attgcgatat gacgtaggat tacattcgat ctcggaacaa tttgacgtga
     4621 ccacttcgac aggtaaattg atgttacaac tccttgggag tttcggtgaa tttgaacgca
     4681 accaaattgc ggagaatgtt caaatgtcaa tgcaaagctt agtgaaagat aaaaagcgtt
     4741 atgcaggagg ccgacgttta ggctatgtca gtggtattga cacgaatggt caaaaacaat
     4801 tggtcattga accagaagag gcgaaaattg ttcgtctgat ctatgcaaaa tatttaggcg
     4861 gagcagggta tcgagcgatt gccaattttt tgaatcgtca aggatatcaa acagtcaaaa
     4921 agaatgcttt ttctaccacg gcagtcaaag atatcttacg caataaaatt tatgcagggt
     4981 atttagaata tgcgcgctac atcaattggg aaaccaaacg acggaagggg aagaatccga
     5041 gaccgatttt agtgaaagga acccatgaac cgattatttc ggaagaagat tatcaaaaag
     5101 tacaagaacg ccttgcttta gaaggaaaac aacccaaatg gaaccataca ggtgaaaatg
     5161 tattgacagg cttgttacgt tgtccagaat gtggcgcacc gatggcagcg agtaatgtca
     5221 caaataccct aaaggatggc acaaagaaac ggattcgata ttattcttgt tcagtctttc
     5281 ggaataaagg agcaagtgtg tgtcatgcca attcgattcg tgccgaacaa gcggaaacat
     5341 ttgtcgccga ccgattaaag gagatcgttc agctaccaga agttcttccc cgattggtgg
     5401 cagcgttaaa tgaagaaata gtgcgacaaa gccaaccatt ggaacaagaa ctcgtggtgt
     5461 tactggaacg aaaggaagaa ttgaagacta aaattgaaaa atgggaagct gctttggaag
     5521 atagtcctga attgtttccg atgttaaaag accgcttaga cgaattgact gaaaaacgac
     5581 gacagttgca cattcgagaa aatgaaatct tagggatctt tcaacaagga gagccgattc
     5641 aagtcaaaga tgtccaacgg atattgacta gtttggatcg ttttcttgcc cagagtgaga
     5701 agaagcaaat caaagccttg tatcgtacct ttatagagaa aataaccttt gatcctaacc
     5761 ataaggagat gttggaaatt acgatgaaat tcacgcatgc cgtggtacaa caattgaatg
     5821 agcaatatca aatagccgta tccaaaacaa aggatacggc tatttttgtg ttaaaaacac
     5881 cctttacgtt aacgatctag tctaggttat atcaaacaac gttagagggg cgctaacgct
     5941 tctctagcga tttgaaatcg ttagagaagt agttagggag gtccttatca taaataggga
     6001 tatttattaa tgaatatttg aaatataatt gtatataaga attaaaaaga gtggggatag
     6061 atatgaaaaa gatgggtaag tgtattttat gtgaaaaata cactgaactt actaaagaac
     6121 atgtaccacc taaaaaatca gggaatactg gaggtaaaaa gaccagaact ggtaatttag
     6181 atgatttcct taagagcgat tttacaaaag gtgattttcc gaaggggatt aaaaggaaac
     6241 ctcaaggaaa tgtttattat a
//
LOCUS       DACDHJ010000136         6217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDHJ010000136 DACDHJ010000000
VERSION     DACDHJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.138"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(13..201)
                     /locus_tag="IV223_001994"
     CDS             complement(13..201)
                     /locus_tag="IV223_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8712257.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            471..833
                     /locus_tag="IV223_001995"
     CDS             471..833
                     /locus_tag="IV223_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8712258.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            885..2567
                     /locus_tag="IV223_001996"
     CDS             885..2567
                     /locus_tag="IV223_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8712259.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2684..4720
                     /gene="recG"
                     /locus_tag="IV223_001997"
     CDS             2684..4720
                     /gene="recG"
                     /locus_tag="IV223_001997"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8712260.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4769..5770
                     /gene="plsX"
                     /locus_tag="IV223_001998"
     CDS             4769..5770
                     /gene="plsX"
                     /locus_tag="IV223_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8712261.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5892..6134
                     /gene="acpP"
                     /locus_tag="IV223_001999"
     CDS             5892..6134
                     /gene="acpP"
                     /locus_tag="IV223_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8712262.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2065 a         1080 c         1422 g         1650 t
ORIGIN      
        1 agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag ttgacaccca
       61 aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag gtttaacagt
      121 acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct tgcgacctgt
      181 aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga gcaatagaat
      241 aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag tacattttca
      301 tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat acctgaaatt
      361 ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg ctatgataaa
      421 atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc atggctgtaa
      481 aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca acagttgttg
      541 gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat caaattaaag
      601 ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt gtccgccaag
      661 aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg aaaatctctg
      721 aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta ttaggcgtaa
      781 ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac taggcaggag
      841 actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt gtaacagaaa
      901 taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg caagtaaatg
      961 cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg ggaacaaata
     1021 tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca gaaaaagtag
     1081 gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga aattccggcg
     1141 ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt gtctcattaa
     1201 atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat aaagcagtaa
     1261 tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa gctggagaaa
     1321 agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta aaaggagcaa
     1381 agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc ggtgtcgtag
     1441 acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca ttgaatggag
     1501 aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat atggtcaatg
     1561 cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat tttggttact
     1621 gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca ttcgattacg
     1681 aagaattccg taactattta gatggtatag gagattcttt acttgtggtc aatgatgacg
     1741 agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat tatggacaaa
     1801 aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat gagacgattt
     1861 tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt atcccgtatg
     1921 gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt ttaggcgcta
     1981 attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt gttaaagcga
     2041 ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac atcttcatgg
     2101 cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct tcaagaacga
     2161 tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta gaagaaaaca
     2221 aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact cacgcgatca
     2281 gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc atgatcgatg
     2341 gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca ctagaacaaa
     2401 tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga acagttgaac
     2461 aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa atcgagatcc
     2521 accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt ctgtgtcaga
     2581 atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc aggtatgatc
     2641 gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt ctttagatga
     2701 tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg ccagcttggg
     2761 catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag atattcagga
     2821 aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa tcgtagtctc
     2881 gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc gtatgatgca
     2941 agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag aaaaagtcat
     3001 tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag ccttgaacgg
     3061 gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc acgtcaacaa
     3121 atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag aatacggcac
     3181 attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc ttgaccgagc
     3241 ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc aagccaaacg
     3301 acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat tgaaaaaagc
     3361 agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat tgaaacaatt
     3421 tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga atgaaatctg
     3481 tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg taggtagcgg
     3541 aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt tccaaggtgc
     3601 gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc aacagctgtt
     3661 tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa aagaacgacg
     3721 tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca cacatgcatt
     3781 gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg agcagcaccg
     3841 ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag atgtcttatt
     3901 tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg agatggatgt
     3961 atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct gggtaagaac
     4021 acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag gacatcaaat
     4081 gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa atgcagtgga
     4141 aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct tgttacatgg
     4201 taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca accagatgca
     4261 gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg cgaccgtcat
     4321 gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc gtggacgtgt
     4381 tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa atgaactagg
     4441 tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa gcgaaaaaga
     4501 tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc tgcctcaatt
     4561 tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag aagaggctgc
     4621 agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt tagcgcaaag
     4681 cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc tatactttat
     4741 actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa tgggcggaga
     4801 tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt ttccagaagt
     4861 agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa atgaaaaaaa
     4921 catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag taaaagcaat
     4981 tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa acggtgaagc
     5041 agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt tgttcatcgt
     5101 aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag tagtagggaa
     5161 agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca aaccagaaca
     5221 tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag gtatcggacg
     5281 tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg aagtgacgaa
     5341 aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa acgtagaagc
     5401 tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta caggcaacgc
     5461 tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa aatctgctat
     5521 tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg gcttacgttc
     5581 tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg gcttgaaagc
     5641 accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata cgatccgcca
     5701 aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat ttgaaaaaag
     5761 cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag gtggacattc
     5821 ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta tgattcttgg
     5881 aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt atttcaaatc
     5941 attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat gatttaaaag
     6001 ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc ggtacggaaa
     6061 tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat tatatcagca
     6121 gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat gcagcttttt
     6181 atccttcttt ttttaatctt tttgacacgt aaaagtc
//
LOCUS       DACDHJ010000137         6145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDHJ010000137 DACDHJ010000000
VERSION     DACDHJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.139"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..1035
                     /locus_tag="IV223_002000"
     CDS             25..1035
                     /locus_tag="IV223_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8712263.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1104..2048)
                     /locus_tag="IV223_002001"
     CDS             complement(1104..2048)
                     /locus_tag="IV223_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8712264.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2032..4752)
                     /locus_tag="IV223_002002"
     CDS             complement(2032..4752)
                     /locus_tag="IV223_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8712265.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4749..5969)
                     /locus_tag="IV223_002003"
     CDS             complement(4749..5969)
                     /locus_tag="IV223_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8712266.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
BASE COUNT         1790 a         1189 c         1017 g         2149 t
ORIGIN      
        1 ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc agctactagt
       61 gcgttggctg ttttaactct agcagcttgt tcaggcgata ctaataaaga tatcgcaacg
      121 atgaaaggcg gaacgatcac tgtatctgat ttctatgatg aagcaaaact tgaatcatcg
      181 aaccaatcct tagtacaacg gatgatcatt tataaagtat tcaataacaa atatggcgat
      241 aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc attaggtgat
      301 acttttgaat cacaattaga agctgctgga tatacgaaag atacttataa agaatatatt
      361 cgtaacaatt tagcttttga agctggcttg aaagctcacg tagatattac agatgacgat
      421 ttgaaaactg cttggaaatc tttccatcca gaagtagaag cacaaatcat caaattatca
      481 agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga tttctctaaa
      541 ttagccaaag acaaatcaac tgatacagag acaaaagaag atggcggtaa agttaaattc
      601 gactcaacga caacaactat tcctgcagaa gtaaaagaag cagctttcaa attgaaagac
      661 ggtgagatct ccgatgttat tactacaaca aatcctactt cttatgcgac agaatactac
      721 gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata caaagatcaa
      781 ctaaaagata tcgctactga aactaaacta agtgacaatg cattcacaac taaagtaatc
      841 ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg atgcctttga aaatgtgttg
      901 agtgcattca caacaacttc atcaagtacg aaagactcaa gtgaaactac agcttcaaca
      961 aagagttctg acacaaaatc aactgattca acaaaagaat cttcaactga agaaacaact
     1021 gattcatcaa aataaaagaa aacaaaaaaa gcgaagactt atgtctcgct ttttttgttt
     1081 tgaatgaaaa aaataaattt ttgctataaa tcttttggta catagaaact tcggttgtcc
     1141 ataccgaaaa tccgatcggt gtattttcct ggttctgttt gacgaatcga agtcaacatc
     1201 atctgcatcg aagcatcgat attgtcgatt tgatgtaata tttctgcttc cattattctt
     1261 ggacgtacag gtgagccata ttctaataag ccatggtgtg ctaaaaccat atgacgtaat
     1321 acgagaacat cttcgtcagc ttcgctgatt ttcaacgcaa gacaggcttt agttatttct
     1381 tcgtcaatca ataccagatg accgatcaaa tttcctgcaa gcgtatattc tgtcgacata
     1441 ggaccggaaa gttcaatcac ctttcctaaa tcatgcaaaa tgataccagc atacaacaat
     1501 gctcgattca gctcaggata ttcatcggcg attgatttcc ctaagtgaag cattgtcaaa
     1561 gtatgataag ctaatccact ggcaaatgca tgatggtttc tttttgctgc cggaaactca
     1621 aaaaattgtt tttgatattt attaagtaga tagcgaacaa tacgattcca atgcgcattg
     1681 gtgatctcaa acaacaattg attgatttct tcttccattt cttctttctt caaaggggct
     1741 ctttccatgt ataaactagg ctctcctggc tcatctaccc ttgttaagcg catatgaaga
     1801 atctttactt gagggtttcc ttgataaacc tctcttttac cgtttagaaa aacgactgtg
     1861 ccggctgtaa atttattgat ttcctcctcc gaagcatccc agtatttacc atcgatagta
     1921 cctgaagtat cttggaaagt aaaagcaata aatttttttc cgtttttagc gattcttaca
     1981 tctgcacttt tgatcaggac aaattgttca aactgctcat cgattactaa atcacgaatt
     2041 tttttcatta tttgctcctt ctaattgttt tacgatttgg ttcatctcat ggtaatagct
     2101 taccatttct ttatcagaag aaaaacaaat tatttgatac ttttctccaa atttagctaa
     2161 aagttcagct aattgatact ttcgctgatg atcataatga agccagccat catcaatgat
     2221 gatcgggcat atcgaacggt tagcttctaa tgagaggtaa gcaaatctca tcgccataat
     2281 cagctgatct ttcgtacctg tagataactc atagatggga aattctgtat atccgtctgt
     2341 taaagtcagt gtatcatttt tcagtaatat cttctgataa tttccttttg ttaaaatacc
     2401 taaataatca cctgctgctc ttaacaactg gggtaattgt ctttcactca tctctgttga
     2461 aagatcacct aagaatgtcc cggcgatttc aagtgctccc cattcctcca gtaattcctg
     2521 taattttgct ttttgccggc tagcttgttg ataaagttcg tccaatgtgc cgtctctttg
     2581 caactcttct acttgaagtt ggagtcgttg ccgattttca cttgcttctt tttcttcttg
     2641 ctggagttgg gattgctttt ctttcagttg gcgcagtctt acagataatt tctcttttgt
     2701 cagctcttca ggaaatagtg gagtaagcat ttccgataat tcattgtatc ttttcatctg
     2761 ctgttcggct tgtttggctt tttgtaaaaa gacaggtact tccgaaggat aagataagcc
     2821 tgcttgagtc aattgttcgc tgtattcttg tatccatact ttttgtttct ttttcaattg
     2881 attgatttct tgcttcaata aagtattttg ctgatagctt ctggcaaatt tcatctgttc
     2941 catctcttgt ataaaacggg aaagcaatac cattttttct gaaattgtct tatcttgtat
     3001 cggtaaccat tctttaacaa actcaaattt ttggtcgatt tgtcgtagtt tttcttgtat
     3061 ttcctgttct cttttatgtt tccgctcaaa tacgccatat aatttctgat aatctatgac
     3121 ttcctgtcca tattctttca tgagtttgag cgtatccatc tttcctaagt gccgatgatc
     3181 cgcaaaacga tatacttctt gctgaagaat ctgtttttct tctccagttc tcttttgttt
     3241 ttcttctgct tcagctaatt gagctaaggc tagatcaagt tgtcctagtt tttcttgcca
     3301 cattcctttt atctcagaga aatcattttt agttttaatt tttggttgcc ttttgtagaa
     3361 gaaatagcca atcatgcctg caccaattag taaaaggatc actttcaagg gcattggtac
     3421 aaatatacct gcaatgatta ttgctccgcc agccatcagc atatacggtt tagatgaaga
     3481 gactggtgct atctctgact gattttgtaa taatgctgga taggtctctt ccaagtcatt
     3541 cacttctgct tcaatcatct cttgctgttc tttagctatt tggagacgca cagaaatctg
     3601 gctttctaga tcagcaatcc ttctttcttc agccagaaga tgcttccatt cttcttcata
     3661 taaaaattct tccggtaacg gatttttctg ccaatgccac ctttgttcca aaatcttcaa
     3721 ttctgaattt gtttctctag tctcaatttg taatctttgc cattcttcgt ttaattgttc
     3781 cgctgtaaat tgaagttgct ggagatcttt tagttcctgc tcctgctcta aataaaaata
     3841 atatctggct gactgctgat ccatcccgct atgcttttcc agtgttgctt ctaattcttc
     3901 caaacgctca tttgtttgct gataccgttt ggagaaagac tccagatcta aaagaagttg
     3961 ctcatcagga agacctgtag tgctgttttt tttgagagat tgccattctt catataaaga
     4021 aaaattaagt tcttgctgtc tggctaatga aaattgtttg ttcgcctctt ttaattgtgc
     4081 caataactgc tgctgttgcg actcgtattc ctgttcttgt tggatcaact tgccaaaata
     4141 agcttcctgt tcttgttttt gatgaatttt ttcttgcagc tctttccatt catttaattt
     4201 ttgattgatt agtaattttt ggcctttttt cttgaataat tgttgtgctt tctgataata
     4261 ttcttgccgc ttttggaata cttgagaact gcccgtgatg cctaaagaaa tcagcgcatc
     4321 atgtagctct ttttctctca aatgatctag ttccatcaac tgctcttgct ggaaagtaaa
     4381 tacctgctgg aataactctt tcgtcaaagg tgcgatcagt ttttctagta actcatcact
     4441 tcccacttga tcccccagcc acaccttcga ttttccgcga ttttgctgct tgtagcgctc
     4501 gacagcaaat tcaccatata caggatgttt aagccatatt ttgccgccat aggctgagcc
     4561 gtcttttggt gtataatcct tttttttacg gccttttgaa gggaaaccaa ataatattgc
     4621 ttgaataaat tgataaatcg ttgattttcc tgcttcgttt cccccataga tcagctgatt
     4681 gcctgaataa aagtcaaaac ttttttgacg ccattttcca aaagcagcaa tctcgatcct
     4741 taaaatcttc attcctgatc ctcctcaaat aaaaaatctt gattaagcag ctgcttggct
     4801 ttttcaagtg tttcttcttg atattccggc agttcgctca atattctagc cgcttcttga
     4861 tgactatata cttcatttaa aatttgttgg aaatcttgag gtgtctgata gtattgaaat
     4921 aactgttcca tcaatttagc agaagcagca agtggggtct tcgtataaaa agtattctct
     4981 atgagagaaa tacgccaaat aaagaaatca tttatcctat ctgagaattc ctctaacaaa
     5041 tagtctaata gctctcctga ttgtatccgt tccaaaacat ctgtattgag atggtcataa
     5101 tcttttaatt ttatctcaat taaactaaat ccatcctcta cttttgaaag ctgctgacga
     5161 atgtgaacaa tgacctcttt tgtcgttctt gctttcctca atgatatttc ctttgtcttc
     5221 caatagacct ctgctacttt aatcggatgt atttggcaac ttcctttact taattcaact
     5281 agtagaatag atgtagaagc ttgttccttt ttagtatgac cttgaggcgc acctggataa
     5341 acaattgttt gcttttcatt taagaccgtt ggcacatgga tatgccctaa agcccaataa
     5401 tcgtatccct tttcttgcat ctttgagggt tggaaaggag catagtttcc tttttgagag
     5461 acgccaggtt ccccgtggta caacccaata tggtaatctg tgagttctct agaaggaaat
     5521 tccatcactt tgtctttttg aatccacggt tgacgataac taaatccact gagagagaca
     5581 gtttctccac tttttatcgt tatttttttt gtctctaccg tttcggatgt aaataaatgg
     5641 acatttttag gaaaggagaa ccaataccgc tctttttgat agaaatcatg atttccgaat
     5701 attataaata caggtatatg gttcttttct aaccgtttca tctgttctga aaaatgtttc
     5761 tgtattttta atgaaggacg attttgatga aaattatctc ctgcaagtaa aacaaaatca
     5821 acgttattta ttattgcctg ttcaatgata tttgataaaa cggtcagatt cgtcttcaat
     5881 agtttttcct gtacagtttt atcaagagtt gtcagtcctt caaatgaccg atccatatgt
     5941 aaatctgccg catggataaa tttcaccatt aaaaatttcc tccttttttt cttttatcat
     6001 accattttca atgttttttt tcatcaataa ccgaaaattt gttcgtatta tgcaaagaaa
     6061 ttacgataaa tggccgactt caaaaaataa gtttattcga aaaaaagatt gtgacatgat
     6121 ttttgtcaca atcctaaata tctat
//
LOCUS       DACDHJ010000138         6080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDHJ010000138 DACDHJ010000000
VERSION     DACDHJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.140"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..330)
                     /locus_tag="IV223_002004"
     CDS             complement(<1..330)
                     /locus_tag="IV223_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712267.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSY"
     gene            complement(430..939)
                     /locus_tag="IV223_002005"
     CDS             complement(430..939)
                     /locus_tag="IV223_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8712268.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1140..2009)
                     /locus_tag="IV223_002006"
     CDS             complement(1140..2009)
                     /locus_tag="IV223_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8712269.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2029..3150)
                     /locus_tag="IV223_002007"
     CDS             complement(2029..3150)
                     /locus_tag="IV223_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8712270.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3152..3547)
                     /locus_tag="IV223_002008"
     CDS             complement(3152..3547)
                     /locus_tag="IV223_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8712271.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3566..4363)
                     /locus_tag="IV223_002009"
     CDS             complement(3566..4363)
                     /locus_tag="IV223_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8712272.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4365..5153)
                     /locus_tag="IV223_002010"
     CDS             complement(4365..5153)
                     /locus_tag="IV223_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8712273.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5167..5643)
                     /locus_tag="IV223_002011"
     CDS             complement(5167..5643)
                     /locus_tag="IV223_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8712274.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5715..>6080)
                     /locus_tag="IV223_002012"
     CDS             complement(5715..>6080)
                     /locus_tag="IV223_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712275.1"
                     /translation="VLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGI
                     SFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYY
                     SVDIPIHEVKYVYDYVKRA"
BASE COUNT         1874 a         1317 c          869 g         2020 t
ORIGIN      
        1 gtatgacctc atttcaccta atgaatcaat tatttcttgc cctttagctc taatatattg
       61 atctctatta cctatctcac tcttatttaa caaaagtttt ttcatgtcaa atttaatcgg
      121 ctgttttatt tttgtaaatt cgcttctact actaatccaa tccgagatta atttttcaac
      181 atgtcccaga gtaaactctg atttattcgc taaatacttt ttttgttgta ttaaagacat
      241 ctttccccga agctcaaaat tgagcctaca tcgattctct atctcttcaa aaagcatctt
      301 atcttcatca ttcttatatt catcattcat aaaaccaatc cagctcctcg attaagataa
      361 tattttaatc tcatacaaaa actagctctc atcaaaacaa ccattgtttt gatttctcat
      421 gaaatttctt tatacttttc tccgattata aattcctaac cccttgagaa aattacgcat
      481 atatttatct ccgcaaggct taaaatttcg ttggccttct tttctcaaaa atgccccgat
      541 ttcgcctttt gatggataga tttctgcctc agcaaaaacg tctaaaatat catcagtggt
      601 catagacaag gcgattttta atttttttag caacacattg ttgatatatt tgatttcctt
      661 gcttttttgc ggcgtgtcta ctatcggctt atctttttgc ggaccacgcg ccaagagaat
      721 caggccattt aggaatgcgt caaaaacttg gttgttgatg gttttttcat acacattatt
      781 ttctgcatta tctttgttct tatcctgtac tttggtcagt accgcctgag cttcttcttt
      841 agtcagtggt acaccgccta gctcaaaagc tttaatcaaa tccgtatctt tcaaatcaat
      901 cgcataacgc aatcgcaata acctatcatt gttattcatt tgttcctccg aagtatttag
      961 tctttacttt cttacgtact ttttgtcttg ctaagatttt taggatcaat gataaaaacc
     1021 gcatcactta tgataaggcg ctgggtatca tgagtgaatg atattgctga atacaaatag
     1081 tccgaccaca ttaagttgat cggacttcat aagcatttta ttgactaata cttttttgct
     1141 taagaattga ttctaacatc ttttgctaaa gttaagcatc ctatagttcc tgcattatct
     1201 gctaacttag gtgtgacaat gtattcttcg atatttggcg tatcgacata gccgttaatt
     1261 agttctaaaa aacgtctttg tactttcttt ttcagatgtt cttgttgcat gacaccacca
     1321 ccaaaaatta taatatctgg tgaaagcatc agggtcgtat tacaggcaca ttgtgcaata
     1381 taatccgcct cgatttccca gaacggatct tctatagaaa gatcttgtcc cttggttccc
     1441 gtacgcatct cgatagcggg tccagatgcc aacccttcga gacaatcatg atgaaaagga
     1501 caagtaccag agaaagtatc tcctgaatgt ctcttaacta acgtatgccc catttctgga
     1561 tggctaaacc cttcaacaaa aatcccatct tgtatagctc ctccaccaat tccagttcca
     1621 acagtgtaat aaacaacact ggataaacct tttccatgac ctgaaacata ctccccgtaa
     1681 caagcagcat tcacatcggt tgtccaggca attggaattt cgaaatgctt attcaacgtt
     1741 ccaacaaaat caaaattttt ccaggcagtt tttggagtag aggtgatatg tccataggtc
     1801 tttgattcac gatggatatc aatcggtcca aatgacccta ctccaatgct ttctaattgt
     1861 tctttatagt gattaaagaa atcaattacc aaactcattg tttcattcgg tgtagttgta
     1921 ggaaaactca ctctttctaa aacctttaaa tcatcattgc caatggcaca aacaaattta
     1981 gtcccacctg cttctattga gccatacatt tcttactcca ccgtcctttt atctaggaat
     2041 tcgttcttcc ctgtctaata gtttttcgaa aaactcatgt ggttcctttt ggtaccaata
     2101 ggcaacagaa gaaatatcgt cttgacgctc ataaagcttg atgtcatcat ttcctagggt
     2161 ttggaaagta acacgtaaat tttctttgaa actaatgggg tcgggtaaat gccagcggta
     2221 caacccatgc atcggtaaag catcatcgcc aaaaccatga actggatttg gattaggttt
     2281 tccagcacta aaataatccc gggtgtgatc tcttttagat tgaaaaggat agcccataaa
     2341 tagcgtatta aaattttgag catatgccct ttctccaaag ttctttttat ggaaagccca
     2401 agcgccgcca aaataatctt ctgctcctgt agatgttatt gtagggaaat cttcatctcc
     2461 atctatataa aacttaaatt ctccttcccc ccaccagtat ctttccaatg cgcacaaagc
     2521 aaagtaggtt cctacataat acccagtgcc tttgattcca tcaataaggg tgtaatcttt
     2581 tccttttgtc gtgatgggtt cacggttcca tttagcatga aaatacatcg catcatccgg
     2641 tacttcagtc aatgcataat taaatgtata aaaaatgtgc ttaataaact caggatgttc
     2701 attcgttaaa gtaatcttag ctttttttcg aaatggcatc tcaaaatagc aattcatccc
     2761 tgatgttgga ttcactacga ttggtaagga attcacttcg catcttgtcc caaacccatt
     2821 acaaaaaaaa tctcctaatg gactttctac agaaggagtg gattcatcat cccaataaat
     2881 acgcaaaatt aaatcacgta agacgaaact cccagcgatt gttttgtctg tcactgtcat
     2941 ccaaaaatga cgtaaaattc cagggccttc catttctgct aaagtaattg tctctccagc
     3001 atttatcgga acagaaggtg acccttttct cccaattcct agatgactcg ctgtcattgc
     3061 cgctttccct ttttcaccat ttttattttc tggtgaaata gttctacttt cctctccctt
     3121 gtggagccat atattattta aacaattcat tttattcgtc ctctatcctt tcttcacttt
     3181 tttgattatt catatagatg atttgttgtt gtgcctccgc aataattgct ccaatatcta
     3241 aattatcatt tcctaactgc aaagcgattg acattgccaa gggaagattc attcctgcaa
     3301 ttacgtgcac tctgtcagaa atataggtaa aaaacttttg atttacactt cctccagcca
     3361 tgtcagttaa aacaattatt tcgtctgtag tatccactaa ctctttaatt tgttcatcta
     3421 tatcatcaat ttcttcgaca tatgcacata agctactaat ttgattatgc atacctgtta
     3481 aaaactctag tgtctcttta aaaccctcag ctagtattga atggctagca atgatcaatc
     3541 ttctcataat tcaaatcctt ttcctttacg ctaatattcc aaatgcagcc ataaccatcg
     3601 caaaaacaat aacgagtaga atcaaaatgg tcatactgat ttttttctta ctgagtagat
     3661 aatatgctgc agcagtaata attactggaa ttaatgttgg taaaattttg tccaacattg
     3721 gttggatttt catactaact tcaccaaaag tgaatttaag tggcgttgaa acactgataa
     3781 ctgaagcaat taatgatcca actactgtta gccccaatac tgaagcagct tcagttaaat
     3841 aattaatctt ttcaccgtat tcagtaacaa tcttcattcc ctgcttataa ccaaattcaa
     3901 agcctctcat tcggaaaaag aagattacca tgattaaaat gaaccataaa aagattccta
     3961 caggattatt ttctaaagcc atataagccg caattgatcc catgatggtt gggatcatcg
     4021 tcatcaaaag agagtcccca ataccagcaa ttggacccat cgtaccaact ttaaagtcat
     4081 taatcgcatc ttttgcttct aatccttgct tttcctccat tgcaacacaa gcacctaaaa
     4141 taatggctgc cagccaaggc tgtgtattgt aatactgaaa gtgattattt aaagcttcga
     4201 tgtattcatc atcatctttg tataattttc ttaaaatagg tgacaatgcg taagtcacag
     4261 atgcaccttg ctgtgtttga taattaaagg tacaaactga catcaaccaa cgatagttcg
     4321 ctttcctaag atcctttttc gttacttgat atttttcgct cattttattc atcctcccca
     4381 ataaacccaa catcagaagc tcccgcagaa gcagtgattt gtgcatcttt ttcagctcta
     4441 tgatacacat taaatgctaa agcgaatcct atgatcgccg ctccaataat aggtaccttc
     4501 aaaaaagcgg ctaagaaaaa tccaacaagt aaataactaa tgtatcgacc tgtaggcata
     4561 aagcgtaaca acatcgcaat accaacagca ggcaacattt ttccggctaa tgttagccca
     4621 tccataaacc aattaggtaa aacatccaaa acgtaattaa cagcagactg tcctaaagta
     4681 acggcaataa atactggaat tgctgcagat aaaccaaaga aaattggccc caaccacaaa
     4741 acactattca ttttcttaaa ttctcgttta ttcgcatatt cctgtgattt gcgaacaaca
     4801 aaaccattta agattttgac aattacatct agttgcaccc ctaacatacc tacaggtagt
     4861 cctattgcta atccggcttc tactccaagc tggctaccaa atgccgttgc aataattgcg
     4921 cacgttccat agtccggtac agatgagcca ccaaaaccgg caactcctaa tgacatcaat
     4981 tgaactgtac cgccaactat caaacctgtt tttatatcac ccatcaaaat tcctgtgaat
     5041 aatccccaaa aaactgcgta atttacaaag atcatcggaa tcccataata atcaacatgt
     5101 ttaataaatg ctaatatagt cagcataata atttgaaaaa tagtaaaatg cattatcctt
     5161 actcctttat cctaattttg attgaatttc ctgtgcctta tccgccggaa caaattgtgt
     5221 aacaatttct acaccagaat ccaacaattt tttatacgtg ctgatatttt catctgatgc
     5281 ataggctcta gaagatagct gtaatgcatt atctttaaca acagtaccgc caacaattag
     5341 tcggtcaatt ctaataccgt tatctactaa atgaaggatt gtttccggct ctttcacgat
     5401 gacaaatact ttttcctgat cgtatttccc ctttttaaaa ttcgcaatcg ctgtttcttc
     5461 agtgataatt gatacagcca ttccagcagg tctagctaaa cgcatacttg atttttggat
     5521 gtcgttattt gcgacagaat catcgatcac catcactctg tttgctttag acattggtgc
     5581 ccattgcgtg acaataatcc catgcaataa acggttatca actctcgaca taacaatact
     5641 cataaatact tcctccttta tctttcattt tcttacaagc aattaccatg ccaaaacaaa
     5701 aagaacttga taaatcaagc tctttttaca taatcataaa cgtatttaac ctcatgaatc
     5761 gggatgtcga cactataata atgttcaacg acactgaaag attgcttaaa atgtgtctga
     5821 aatttttgaa attcctcact cgtttcattg aaataaattt catcttccaa agtattttgt
     5881 tttactaatc gttcaattaa gcaactaatg tgaacatata atccgaaaca cgtattatta
     5941 tcaaatgaaa ttcctagatc attttgtaag atgtatatag catctgaaac ccgttctaat
     6001 aatttagtcg gatttaatat ggtcaattgc tccattaaat tcgttagact aaaattcttc
     6061 agaatattat tgttcaaaac
//
LOCUS       DACDHJ010000139         6048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDHJ010000139 DACDHJ010000000
VERSION     DACDHJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.141"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            147..396
                     /locus_tag="IV223_002013"
                     /pseudo
     CDS             147..396
                     /locus_tag="IV223_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(640..1470)
                     /locus_tag="IV223_002014"
     CDS             complement(640..1470)
                     /locus_tag="IV223_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8712276.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            1678..3012
                     /locus_tag="IV223_002015"
     CDS             1678..3012
                     /locus_tag="IV223_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8712277.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3053..3964
                     /locus_tag="IV223_002016"
     CDS             3053..3964
                     /locus_tag="IV223_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8712278.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            3961..4815
                     /locus_tag="IV223_002017"
     CDS             3961..4815
                     /locus_tag="IV223_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8712279.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            4812..5429
                     /locus_tag="IV223_002018"
     CDS             4812..5429
                     /locus_tag="IV223_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8712280.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            5431..5949
                     /locus_tag="IV223_002019"
     CDS             5431..5949
                     /locus_tag="IV223_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8712281.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1797 a         1087 c         1222 g         1942 t
ORIGIN      
        1 aaacccgttg tttctgtcag tgaagcagcg ggtttttaga tttattgtct ttagacctaa
       61 ttgagcgcgc cgagcgattg aaatacaaaa ccacttgcta tgcgggtgga gcaataaagg
      121 gaaatagttt agcccgtaca aagtagatgt gtaaatacca cattgtattc acaccaaagt
      181 atagaagaaa aataatttat aatcaatatc gagccaatat tcaggagatc atcaagagat
      241 tgtgcaaatc taaaatataa atttgggtat cgacactttt gggtggaagg atactatgtg
      301 acaaaagtag gacaaaatga agcgaccatc aaaaagtaca ttcaagaaca agaaaaacat
      361 gatagtgcgt tagataagct gagtgtacga gagtaagacg atccttttaa gggctaagga
      421 gtagtacata aaccttttaa gaggtgggca aaactggcaa aggcaatatg gcttgaacat
      481 aaaatatgaa agccagcgtc tttaggcgct ggccggtgat ttgagcttat agctctagag
      541 aaaaccaccc ttttgaaggg tggttatgat taatgacttt agtcagtttt gctcacaaag
      601 cgtgtggaac aaaaaacggg tgtttctacg ggtagatatt tatttcttga atggaggtgt
      661 cccatacatt tttggtgtta ctggttctaa ttttggtgca gcgccttcga tcttaggttc
      721 tttcaaccat tgatattcgc cttcgccatc cagtgctttg ccactagccc aagaaccttc
      781 actactttgg tctccctctg agaagttata gaagtcataa gcaatgtctt ttcgttcttt
      841 gtcacgcggg aaagtacttg ggactaagat tgaaccttct ttttcttcta attctttcac
      901 tgcacattcc catagatttt gatgatagct atctcttgca aggagaacag acagcatatc
      961 ttttacgcca cgatcatcga tcatttcgta aatacgtgct acttgaagtc ttccttgtga
     1021 ttcggcattt aaattagctc taaagtctgc taacaagttg ccactcgcaa tgatataggc
     1081 accgctccat ggattgccca cgctgtcagc aggacgaggt cctaagccag ctacgatcgc
     1141 gtgctgtgga ttcattccag agataatggc accgattgtt ggatcttttt gcatcgctgc
     1201 ttcttgatct tcaacaggcg caccctctaa taaacgagca accattgtcg ccaacatttc
     1261 cacgtggccg atttcttctg ttgcaatgtc catcagcata tctttatatt tttcttctcc
     1321 tcggcagctc cagccttgga acaggtactg catagctaca ctcatctcgc catattggcc
     1381 gccgatcagc tcctgcagat atttcgcgat taatggattc ggacgatctg gttttgcttc
     1441 aaactgcaat tctttctggt gtctaaacat caaaattcct cctaatctat ttttaataca
     1501 ttctcattct gctacgaaag attaaaaaat tcaaagaaag cgctcgcgaa ttatataaag
     1561 tattggtatc attaataatt tttttagaat cataccttat aaattgttat tttcagaaca
     1621 aaatgatatc gttatattaa aaccggcatc ggttacatta aaatttggag gtaaaatatg
     1681 aaaaaattat tgtttacagg gttgctggca agttcagcat tgtttttatt aggaggatgc
     1741 ggatcaagta atgcaagcga cgccagttca agtgcagcaa attcttctga aaaaacaagt
     1801 gatggaagaa aaaatattga tttctggtcc ttttggggat ccggtgcacg aaaagaagct
     1861 atcgaagaaa tcattgagga tttcaatcaa tcacaaaacg agatccatgt aaattataag
     1921 tatcagccat ggggtgacat ctggactaaa tctttatctg caataacagc tggaaatgca
     1981 cccgatgtta ttgtacagga cataaattcc gttgcccaaa gagcagaagc acagcaagca
     2041 actaatctct ccaaatatgt agaaaaagac acttctaagg atttttatcc gcagctttgg
     2101 gatacagtag aatatgaagg tgatccttat gcgatcccat tcaatacaga tacacaagtc
     2161 attttttata ataaaaagtt atttaaagaa gctggaatca gtgaacaaga tctaccgaca
     2221 acttgggatg aattagaaga aacagcacgt aaactagata aaaaagaagg aaaagaattt
     2281 acacgcatcg gtttttatcc attatggaat ttaggggctg atgtatgggc gctaaacgca
     2341 gataacggga ctagttggtt tgacaaagat gaaaaagtca agattgatac taaaaataaa
     2401 gtggaagcct tggagtggat ccaagactgg caagattatt atgggaaaaa tacgatcaat
     2461 aaattagaag ccgaatttgg gtctggtgta tctgatccat ttatttccgg attagtagcc
     2521 atgcgtgccc aaaatatcaa ttattactct agtttgatgg aaaatgcacc ggatgatttt
     2581 gattttggcg tgatgcagct cccagaaaaa gaagaaggtt caggccattg gtcgtggggt
     2641 ggcggattcg tcttggaagt accatacgga gcaaaagatc ctgaggcctc ttatgaattc
     2701 atcaaatatc tttcttctcc agaagtacaa gaaaaattcg gcgaaaaaag ttttgatatc
     2761 atggcgagca agacagccaa tgagaattta gtgaataatg acaacctaac ggataaagga
     2821 aaaatgatct atcaaatggc ggacgaaaac tttaaaaata cggtgatcac accagttcct
     2881 ttagatgctc cggattacac gacattagtg aatgaacaga ttgaccagat tcttttagga
     2941 acaaaatcac caaaagaagg attggcagat gcacaaaaag cagtagaaaa cttagtcaag
     3001 caaaatcaat aatccatttt tgactgtcta ggtacagagg aggcaagaaa atttgaagcg
     3061 tttatctatc cgaaagaaaa aagaagcaag atgggcctat ttatttatct cgccttttat
     3121 tatcgggttc ttggtattta tgctagggcc tatgcttttt tcagttattg gtagtttcac
     3181 cgattataat ttgacatcca agatgaattt cattggtcta gcaaatttca aaagaatgtt
     3241 cgttcacgat gatttatttt ggaaatcgtt atacaacacc gtttatttcg tcatcttcaa
     3301 tgtaccgctg acgacgattt tcagtgtgtt tttagccaca gtactcaatc aaaaaatcaa
     3361 aggaatcagt gtattccgta cagctttcta tttaccggca gtcttatcag gagtagcagt
     3421 ctacatctta tggatgcagc tgctttctcc atctgcagga ttgatcaaca catttctggg
     3481 ctggttccat atccctggac cagcttggct gtttgatcca caatggacga aacctgcgtt
     3541 gattttgatg aaaatatgga gttctggagg agcaatgcta ctttacttag ctacattaca
     3601 aaatgttcct agatctttat atgaatcagc agaaatcgat ggagcaggcg tttggggaaa
     3661 gttcaaaagt attactttgc cattaatcac tcctattatt ttttacgatg tgatcacctc
     3721 tacgatcggt tctttccaaa tttttcaaga agcttatgtc atgacgaaaa atggaacagg
     3781 cggacctgca aattctttgc ttttttataa tctgcatatg tggaacaagg cgttcgtagc
     3841 ttttgatatg ggttatgcga tggctatgtc aatgatcctg tttctgatcg tgttggttct
     3901 gacgtttgtc aatttgaagc ttgcacctaa gtgggtctat tattctggag gtgacggcaa
     3961 atgatggatt atttcaaaaa gaccagtgtg aatcggcaaa caaaaaaaat actaagttat
     4021 gtcctcatga cagtaatagg aatcattttg atcattccat tgctttggat ggtgtttact
     4081 tcattgaaac caatggaaga aatcgttcgt tatcctccga cctttttccc agaaaaaatc
     4141 gtatgggaaa actatctgga tacgattgcc gcctttcctt tttggcgcta tgcaagaaat
     4201 acattattca tcactgtgct agtagtgatc ggtaatgtcc tttcaaattc ttttattgct
     4261 tatggatttg cgaagcttga ttttcctggg aaaaagctga tgtttgcttt ggttctttct
     4321 acgatgatga tcccggggtt tgtaacgatg atccctcaat atgtattatt ctccaagatc
     4381 ggctgggtag gtacttattt gcccctgatc gttccatcgt tcttcggcaa tgcgttcaat
     4441 atatttctga tgcgtcaatt ttacttatcg atcaataatg aattgatcga agctgctgaa
     4501 atcgacggag cgaatcattt gtatatttgg agtcatttga tgcttccatt gacgaaacct
     4561 gccttgatta ccattgcgat caattccttc aacgcagctt ggaatgattt cttaggtcct
     4621 ctgttatata tccaagatca agaaaaatac acgttacaga ttggcttgca agtttttcag
     4681 aaccaagcaa cgacacaatg gaattatctg atggcaggag caacacttgt cttgatcccg
     4741 acgattttgc tgttcttttt tgcacaacgt tatttcattg aagggatgga tttgacagga
     4801 ggttcaaaag gatgatgaat cgatcgattg gtaaaaaagc caatgattat ttcttagaaa
     4861 tcattgaatg gattccacgt cttatccatt tacagctact atggttcctg tgcgtcttgc
     4921 ctgttttcac attaggaact gcaagtcgaa cagttctgta tatgatttat cattacaaaa
     4981 aaaatgaaga gaaaaagctc ggttcactat tctggaaaaa gtttcgtgag aacttctctc
     5041 tttatggaaa gcaagatagt ttagcaagtg tttattttct tcttctattg atcgattatc
     5101 agattttccg ctattggggt gggggaattg gctatacatt gatgtatact acgttgtttt
     5161 tgcttctatt aagcgcgctt ctctttagtt acaaaatctt gcttcaactg gaacaaaaag
     5221 aagcttcttg gattactgca tttttcttgt tttttaatga tttcaaaagc gcgcttttct
     5281 atttggcagg cacgttgatc cttcttgtcg cattctggtt tgcaggacca atctattttg
     5341 ctttattagg tttcagtttc ttgttttatt ttcaagtcct gctttttatc catcgtacgc
     5401 aagaaaaggt tcttaaaaag gaggaataac atgttgcgtt tgattgctaa aatcgatgat
     5461 agtattatca acaagttcgc caagcaacgg aaaacggata gtcgaaaaat caattttttg
     5521 aaatttatca gtaatatctc taatggtggt tttatttggt tgctccttgc tgctgtttta
     5581 gtcttgatac ctgatactag agaatatggt ctagccattc ttatcggcag tttgatctat
     5641 ttttttgttg gtttgctttt tatcaaaaaa acgacaagac ggaaaagacc ttatgaaaaa
     5701 aatcgtcggc ttgtccttta cacaagtaaa ccgaagactt cttcttttcc ttcaggacat
     5761 acagcgctag cctttttatc agtagctgcg ttgttcgaat taagtccagt tgcaggaata
     5821 ttagcctttt tatttgcttg tttgatcgct tattcgagga tttttttact tttacattat
     5881 cctactgaca ttttaggagg agctatttta ggaagtgtta ttggcatcct atgcgctgcc
     5941 gttatctgaa ttaattgtac gaataaagca tgagtgcgta aatgcgcatt tgtgctttat
     6001 ttttttgttt cgaggaaagc gtagttgttt attcatttgt cttaataa
//
LOCUS       DACDHJ010000140         6018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDHJ010000140 DACDHJ010000000
VERSION     DACDHJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.142"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..142)
                     /locus_tag="IV223_002020"
     tRNA            complement(59..142)
                     /locus_tag="IV223_002020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:cag)
     gene            670..1170
                     /locus_tag="IV223_002021"
     CDS             670..1170
                     /locus_tag="IV223_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712282.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1360..1704)
                     /locus_tag="IV223_002022"
     CDS             complement(1360..1704)
                     /locus_tag="IV223_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8712283.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1858..2778)
                     /locus_tag="IV223_002023"
     CDS             complement(1858..2778)
                     /locus_tag="IV223_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8712284.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2965..3456
                     /locus_tag="IV223_002024"
     CDS             2965..3456
                     /locus_tag="IV223_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8712285.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3480..5855)
                     /locus_tag="IV223_002025"
     CDS             complement(3480..5855)
                     /locus_tag="IV223_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8712286.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
BASE COUNT         1847 a         1186 c         1004 g         1981 t
ORIGIN      
        1 atgttatttt ctttctcatc gctccaggtg taccttctct ttataaaaaa acaaattatg
       61 cggctaagaa gactcgaact tccacgaggt caccctcact agcgcctgaa gctagcgcgt
      121 ctgccaattc cgccatagcc gcatcacatc ttttagttta ggattacatg tgttttttgt
      181 caagaacata tttttgttct ttttcatttc tgtacgccct cccttatgcc atctacacaa
      241 ataattcatc cgagcaactc catcatctat tgagggttgg tgaaaaaatc tgttttatcc
      301 atatgatcga tttcaatgtt aaacgaacga ctctgggatt agtaactatt ttattttttt
      361 cttcatttct tttttcctct ttttctcgat tcagctatct tctttcattt ggatattgat
      421 tatttcttct gttgattact atccttctgg aattctagac gctgaagtaa ttcacgtttt
      481 ctttcttcta gagatcataa gtaacgaatg aacgaacttc aaataatttt tttattgtaa
      541 ttcatatttg tattgttcga tgattcttaa tacgatatga cacaataaat aaaaataggc
      601 atggctattt acaacaatag tgctatagta gaaaacaaat gaataaggtt acttaaagag
      661 gtgaatacga tgaaactgat caaactatgg gaagcagatt taaagaaagc ttatcagcta
      721 caaactagct ttggaccaaa tgagagtgga tttatcaaca atgcttatgg ctatacattt
      781 gctgaatttt tagattatgt aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct
      841 gagagttacg ttcccgcaac cgtctttatt ttagaaagtg atgaacatga gtatgtagga
      901 atttttaatt tgcgccataa actgaacgaa acattaagaa acggcgctgg ccatattggt
      961 tacggcatct cccctaatta tcgtaaaaaa ggatatgcca cacaaggatt agcattagta
     1021 ctacaagagg caaaaaaaat cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat
     1081 cttggcagtc ttaaagcaca acaaaaaaat ggggctgcga tttatcgtga agatgagaaa
     1141 gaatactata cgaaaatcaa actgatgtaa aaaagaagga ttccaaaagg aaagggattt
     1201 gaatgtcctt tatcaataga acacatttat ttaattaact tataatatag cagacaaaat
     1261 tttataaaca aagaaacctt ttatctcttt tcttctagaa aggataaaag gcttttttgt
     1321 ttttcctatt tgcttctcat agctagtcag ccaagatttc tatcgtatcc ccaacgcaat
     1381 ccgtgcataa cggctcatct tatctgtcgt ccatgctgga taccagacta gtttgacttc
     1441 tgctttcgtt acttctggca cttcttttaa tgcgtcatgg atagattctg ttaaaatatc
     1501 tgctaatgga cagcccatag ttgttagagt catcttaatg actgtttctc cattttcagg
     1561 attgaattct atatcataaa tcaagcctaa attgacgatg tcgattccta attcaggatc
     1621 aatcaccatt tccaaggcag caagaatacg ttctttgatt agttctattt cttgtccaga
     1681 ccgttcttgg tttgcttcgc tcatcgtacg acactcctta acacgatttt tatttatcat
     1741 actctttttt tagctgcttg taaatgagaa tttagaagta agattctcgt ttaaatttat
     1801 aaaaatgttc ttattagatg taatagatta aatttattct aaaataaacc tgattcctca
     1861 ttttttttgt gaacgatttt ttatttctgg caatacgcca gttgttcgat acattttttg
     1921 ttcgttttca tttgcaaacc ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc
     1981 atcgtcgtcg atttcatgaa cgatctgatc aagatcatac atttcgtagc gggtgatgac
     2041 catcatgatc atgttccctt gtttttttga atagccaccg acagacggca gtaatgtcat
     2101 tccacgtacc atccgttttg ctagttcttc tgttacctct tctggcttga cggtaacgat
     2161 cattgctgtt actttttgat gactcgtgtg gatggcatca attacttgag tcattgcata
     2221 aatggaaata atcgtataca aggcactttc ccagttaaag aagaagcctg cgatgactac
     2281 aatgatacca tttagaatca gcatgtagtt tccgactgtt tttccagttg tttgtgaaag
     2341 tacaagagag atgatatcca tcccaccagt tgtaaagccc atttttaaag ataatcctac
     2401 gccaagacct aataaaacac cgcctactag ggcattcatc aaaatattat cagtgatctg
     2461 tccgactgga agcagcatcg tcatcaccga tacacataca acattcacaa aactaaggat
     2521 cgttgctcgt tttcctactt tgagaaaccc taatacaaac acagggatat tcaaaagaaa
     2581 aataaatgtt cctgtgtcta tcttgatatg aaaccattca tataaaagcg aggaaatgat
     2641 ttgagcgatc ccattcatcc ctgcagaaaa gacattggct ggaatcaaaa aataatttaa
     2701 agcgaccgca gcggtcaatc cggttatcaa aataaccact attttcttta atctttcatt
     2761 tttatgatac gcagtcataa ttttactcac aagcacctct cccctacttt ggtattccaa
     2821 atgtaagaat aacagtttat ctattaaaat aaaatacttc gaactcaaat gagaagaatt
     2881 ttagcaaaca ggtaagaatc tgccctaatc ctatgataag atagatgtat ggtaaatatt
     2941 tatatgtata tcaggagggt tcctatggca aaagatgcta gcttcgacat tgtatcagaa
     3001 gtcaatacag aagaagtaaa aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc
     3061 tttgacttca aggattctac agtagaaatc aaattagaga acagcaatct agttgtattg
     3121 agcgatgatg atttcaaaat cgaacagatc aaagatgtat tatttggtaa gctgatcaaa
     3181 agaaatgtgc caatcaaaaa tatccatttt tccgaatcag aacacgctct aggtggcaaa
     3241 gctcgacaaa ctgccgattt ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact
     3301 acagctatca aaaatgaaaa actgaaagta aaagcacaaa tccaagaaga tcagattaga
     3361 gtgacaggta agaaccgtga tgatctacaa gctgtgattg ctttattacg taaacttgac
     3421 ttaccaatcg agttgcaatt cactaactat cgttaatcaa aaaacgaggc tgacgtctgt
     3481 cattcctcgt ttttttgatt tactgcttct tttgataacc aaaaacgctg tattccttct
     3541 tccaattccc caacacttcg tacttctctt gctgtttgcc aatgttgtgg gaataaacgt
     3601 cgataagaaa aactagaaaa tcgttgatct gccaatacaa cgattccttg atccgccatg
     3661 ccacgaatca ctcgaccacc agcttgcaag actttgttca ttccaggaag ctgataagcg
     3721 taagcaaatc caaggtgttc cttctcatca taatatgatt tgatcagttc ctgctcatga
     3781 ttcatctgcg gcaaacctac acctacgatc atactgccga tcaatcggtc ccctctcaaa
     3841 tcgattcctt ctgaaaaaat cccgccaagt acgcaaaatc caatcaatgt ttcagcggga
     3901 tttgtttgaa attttgttaa aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg
     3961 attagcacat tgctgtcggg atattgctgt ctgaatgctt ccaccgcaag atctaaatat
     4021 tgataagaag gaaagaaaac gaaataattc ccgacctttc catcagcaaa agcatgaatc
     4081 gtctctacta attttgccag actgtgttcc ctttttcgat aagtcgtctc taggtaattt
     4141 gcgatgaaaa cttgctggtt tccttctggg aaaggactcg gcagtttgta agccagactt
     4201 ttttgtcccc ccagtgtttc ctgataataa gagagtggtg aaaaactagc agaaaaaaga
     4261 atactgcttt ttcctttatc caacgactgc tcaagaaaca agcttggatc aatacaaaac
     4321 tcttttacta tcagatcgcg atacgttttt tcgactgttg tttcgtagtg atcatcataa
     4381 aattcactga ccttcaaaaa atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt
     4441 gatgggtgct caggaaattc tgccagccat tctttaattt tttcgcttaa ttgataacct
     4501 gcctttacta aagattcagc cggtgctttt tgatgatgat aatcccaatg atcttctttt
     4561 gctatttctc ggatcgaatc aaattctttt aatagtttgt tgaaacggcg atgaagtttt
     4621 ttgaattcct ttggtatagc ttccttactt cgtttcgttt tttcataaga tagttctgcc
     4681 gaatacatct ctcgggaacg attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag
     4741 tcctcgtttt tttcatcttc aaaaaaacgt cttaaataaa cagttggatc aaataaataa
     4801 ttgtaatccc caatgattac gtcacaccag atactgacat ctaatgatag ttcgaaagga
     4861 caaagtgtat gctttcttgc ataagtttcg atgacttcac gagtgatctg attctcatga
     4921 ttcaaaagat cccataaacc ttcattaatc cgattgtaat aaccattggc atatgggcac
     4981 tgatctggat tacaggtcgt ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg
     5041 acacttttta cttcagctcc tgtatctttt aatgctgtca tagcatcttc agctacttgt
     5101 ctagttattg ttttagccgt tagataaaag atcttttctc cctcttcttc ccctacagct
     5161 ttcaaagcgg ggaataacgt agagattgtt tttcctgttc cggtaggtgc ttctacaaac
     5221 aatttctgtt tcgtacggat cgttttataa gcagctaccg ctagttcacg ctgacctttc
     5281 ctatactcat catgggggaa tttcaaagcc atcaaagagg cattgcgtac agtgcgccag
     5341 ttttcttgaa aaacaagcca ttctttataa tcttcagtca gctttttaaa aaaagtttct
     5401 aactgctctc ttgtctgatg ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag
     5461 taagtcaatt gcagatttat ttcttgtaaa tcatgttctt gactatagat ataggcatac
     5521 acccttgctt gatgaaagaa aagttccact tgttctggtt ctaaatcttc aaatcgtggt
     5581 tcggaggttt tgatttcatc tatataataa ataccgtctc ttttgaaaat accgtctgca
     5641 cggccttcaa cgatcaggta atattcatct aattgaactg ttgtttttaa aaagacttct
     5701 ttttcatagt tttctcccgc atctttttgg agcttgcggt ggattctcgc tccttctaat
     5761 gcagtatggt tacttttttt acgattgtcg atacttccac gtcgcaaaat aaattctact
     5821 aagtgccgca cagatatttt tgactgtttg atcatttcca ccacctcttt ctctctaaga
     5881 ttacctgaaa aaacctagtt tagcaaaaag agctctcttt ccaaacataa aaaaacaccc
     5941 tgctgattag gcagggatat aggatataga atttggattt ttggatgaat ctaatgtaac
     6001 aaggacatat taaaatgc
//
LOCUS       DACDHJ010000141         5910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDHJ010000141 DACDHJ010000000
VERSION     DACDHJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.143"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IV223_002026"
     CDS             complement(368..1486)
                     /locus_tag="IV223_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712287.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IV223_002027"
     CDS             1957..3264
                     /locus_tag="IV223_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8712288.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IV223_002028"
     CDS             3383..4582
                     /locus_tag="IV223_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8712289.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IV223_002029"
     CDS             complement(4609..5877)
                     /locus_tag="IV223_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8712290.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1814 a         1252 c         1058 g         1786 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact
//
LOCUS       DACDHJ010000142         5807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDHJ010000142 DACDHJ010000000
VERSION     DACDHJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.144"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..1129
                     /gene="ulaG"
                     /locus_tag="IV223_002030"
     CDS             62..1129
                     /gene="ulaG"
                     /locus_tag="IV223_002030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8712291.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            1147..1608
                     /locus_tag="IV223_002031"
     CDS             1147..1608
                     /locus_tag="IV223_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8712292.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            1628..3112
                     /locus_tag="IV223_002032"
     CDS             1628..3112
                     /locus_tag="IV223_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8712293.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            3155..3454
                     /locus_tag="IV223_002033"
     CDS             3155..3454
                     /locus_tag="IV223_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8712294.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            3469..4107
                     /locus_tag="IV223_002034"
     CDS             3469..4107
                     /locus_tag="IV223_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8712295.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            4112..4972
                     /locus_tag="IV223_002035"
     CDS             4112..4972
                     /locus_tag="IV223_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8712296.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            4962..5672
                     /locus_tag="IV223_002036"
     CDS             4962..5672
                     /locus_tag="IV223_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8712297.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
BASE COUNT         1881 a          937 c         1322 g         1667 t
ORIGIN      
        1 atgctgatac acattaatat ttacttgtaa gttggaacaa cgatatgggg ggagcaagca
       61 aatgagtaaa actgtcaatg aagtaacaaa agaaaagtgg ttagtagaaa catttcctga
      121 gtggggcaca tacttgaatg aagaaatcga aacagaagta gtggatcctg gaacttttgc
      181 tatgtggtgg ctgggctgta cgggaatttg gctgaaaagt catgaaggaa cgaatatttt
      241 atgtgatcta tggtgtggga cgggaaaacg tagtcatggg aatggcatga tgaaaaaagg
      301 ccatcagatg atgcgtatga gtggcgttca aaatatgcag cctaatttac gtactcagcc
      361 tttcgtaatc gatccttttg cgataaaaaa tgtagacgct ttaatcgtta cacatatcca
      421 ttccgatcat ctagatatcc atacagccgc agcagttcat caaaattgtc ctgaagcaac
      481 attcattggg ccaaaagaag tggttcgcac gtggcaaaaa tggggaattc ctgaagaaaa
      541 aacgattaca gtaaagccag gtgatgcagt caaaatcaaa gacatcaatg tagtagcttt
      601 agaagcgttt gatcggacag cgttggtcac ttgtgaagat ccagaagagg tattaaaagg
      661 aaaaatgcct caggagatgg atcaaattgc agtaaactat ttattcgaaa catcaggagg
      721 gaatctttat catgcaggag attcacatta ctccaattta tttgccaaac atggtaatga
      781 acacaaaatc gatgtctgtt taggggccta tggtgaaaac ccacgaggga taacagacaa
      841 agttacttct gttgatatgc tgcgcatggc ggagtcattg aatgcaaaag tagtgattcc
      901 tgtccactac gatatttggt ctaattttat ggctgatcca aaagagatca ctgaaatttg
      961 gaaattcaaa aaggatcgcc tgaagtatgg atttaaacct tatatctggc aagtaggcgg
     1021 gaaattcatc tatccgacaa acaaagatga tcttgagttt aatttttatc gaggatttga
     1081 agatgtcttc tcgattgaaa atgatacacc gtttccgtcg tttttataag aaagaagggg
     1141 agtttcttgc tgaaatactt ttttgaccat caattgattc attacccaga taaagaaatc
     1201 acggattggc gtgaagctat cgccgaaagc tgtcttgtat tgatcgataa ggaaattatt
     1261 gatgaaacgt atgtggagga gatcatccat tgtgtagaaa aatacggtcc ttatattgtg
     1321 atcgcacctc aagtagcaat gccgcattct tcagaaaaaa gtgatggtgt ttttggaaca
     1381 gctatttcat ttaccaagtt gaaacagcca atccgttttg aaagtgaagg ggagaatgag
     1441 gagaaaacgg ctagtttgtt ttttacactc gcagcaaaag atccagaaga acatctagaa
     1501 aatatccaaa aattatccga attattaatg acagatggat tggttgagcg tctcatctct
     1561 acgaactcca tcgatgatta ccaagttgtg atggcagaat ataattaatt gatagaggag
     1621 aaaaaagatg ggggaatttt tgttaagcat ttggacgtat tttgcaacaa atatcttgac
     1681 gcagccagca tacctgattg gttttatcgt gttattaggc tatattttgc tgaaacgccc
     1741 gcttcatgaa tgcatcgctg gattcttaaa ggcaacggtc ggttacttta ttttgactgt
     1801 tggctctggt ggtttggtca ataattttcg gccaatcttg gttggactaa aggaacggtt
     1861 caatttggat gccatggtta cagatcctta ttttggacaa aatgctgtgg atgctggatt
     1921 aatggagaca tttggccgta cctttggaga tgtgatgatt ttattactga ttgcatttgt
     1981 tttaaatatc ttattagtcc gttttcagaa atatacgaaa ctgcgttccg ttttcactac
     2041 cggaaacgtt caggttcaac aagcagctac cgctttttgg attttactgt tttgctttcc
     2101 tgatctaggt aggatacaag cattgatttt tatgggactg attttaggtt gttattggac
     2161 agtagcttct aacttaacag taggaatcac gcaggaattg acagaaggtg ccggatttgc
     2221 agtggcacat cagcaaatgt tcggaatctt tatatttgca aaattagctg agtggatgaa
     2281 aaagcgggat gaaaagaaaa atagatcaat caagcaagac aaaaaaattg aagatatcaa
     2341 attaccagga ttcttgtcga ttttcaatga gaatatggtt gctacttcgt tactcatgct
     2401 atttttcttt gggatcattc tgattgtact aggcaaagat tatttgattc aagcgcaatt
     2461 catgcaagaa gggcagagtt tcttattcta cattatgacg acttctttaa attttgcggt
     2521 atatctggcg atcttgcaat taggtgttcg gacattcgtc gatgaactaa ctcaatcttt
     2581 ccaaggaatc tcaaacacga ttttaccagg agctgttcca ggaattgacg tagctgctac
     2641 ttttggcttt ggttcgccaa atgcggtaac gattgggttc ttgtttggtg ctttgggaca
     2701 gtttttgatg attggtttac tgattttatt gaaatcacca acgatcgtca tagccggttt
     2761 cattccgttg ttctttgata atgccgttat agcagtttat gccaataacc gaggtggatt
     2821 caaagccgct tgcctgttcc cgtttctttc aggaatcata caagtaggcg gttcagcatt
     2881 attcgctaca tggattggga tgtcaacata cggtggttat atcgggatgt tcgactgggc
     2941 aacagcttgg ccattgttta cagtaatcat gaaagtccta ggttatgctg gaattgccgt
     3001 agttgtcatt ggtttattgg ctattccgca gttacaatat cggaaaaatc ctgatggtta
     3061 ttttttgatt gtagaagact atgacacata tgcagaaaag cagagagtat aaaagaaaat
     3121 ctgattagac aacactcata atggagggat taagatgaga attttagtat cgtgtgcaaa
     3181 tggatcagga acaagcttga tgatgatgag aagtgtggaa aaagcaatga aagaactaaa
     3241 cgtaccaatt accaaaatcc atcattgtgc catttcagag ggaaaaagtt cagctggcca
     3301 atacgatgta gtttttacgc cagtcaattt tttgaatatg tttactcagg cagagaaaaa
     3361 aggagttaca gttatcggta tcaaaaatgt gatgtcagtc aaagaaatca gtgaaaaatt
     3421 tgctgaaaca aagtggtacc aaaaaaacaa gtaaaggaag gaactaagat gagcaggccg
     3481 aatttgcaaa ttgcgttaga tcacaatgac ttagaacatg cattaggaga tgttatgaaa
     3541 gttggtgatg tagtagatat tattgaagtg ggcacaattc tttgcttaca ggaaggacaa
     3601 aaagcgattc gctgtattcg cagtatgttc ccagataaaa aactagtcgc tgatacgaaa
     3661 tgtgcagacg ctggaggaac agtggcgagt aatgttgcaa aagcaggagc agactggatg
     3721 acagtcattt gttgtgcaac gattccaact atggaggcag cacaaaaaga aataggtgaa
     3781 ttgcaagtag aactctacgg caattggact tttgaacaag ccatggattg gcataacatc
     3841 ggtatccggc aggtaatcta ccatcaaagc cgtgatgcgc tgttatctgg tgaaacttgg
     3901 ggtgaaaaag atttatcgaa gatcaaaaaa ctgattgaac taggctttaa tgtttctgta
     3961 actggtggat taaatccaca cacattacac ttatttgaag ggatcgatgt gtataccttt
     4021 atcactggtc gtggtattac agcagcaaat gatccgatga aggccgcaca aaacttcaaa
     4081 gatgagatca ttcgtatctg ggggtaagga aatggctcaa attgggattt atgaaaaagc
     4141 gttacccaaa gatatttcat ggaaagaacg tttttccctt gtaaaagaaa tggacttcga
     4201 ttttatcgag atgtcgattg atgagacgga tgagcggtta gcacgattag attggtctga
     4261 agaaaagata gctgagctga gagaagagat gttcagctcc ggtgtacgta tccattcgat
     4321 ttgtttaagt gcgcatcggc gatttccgtt tggttctgct gatccagaaa agaaaaaagc
     4381 agcgaaacaa ctaatgaaaa aagcaatcca tttggcgcat aatctaagcg tcaaagtaat
     4441 ccaaatagct ggatacgatg tttattatga agagaaatca atgaattcca gagaagactt
     4501 tatcgaagga ataaaagaaa gcgtaaagga agcttctaaa tatggcatca ttctttccgt
     4561 ggaaatcatg gatgatccat ttatgaactc catttcaaaa tttttagaaa tcaaagaaca
     4621 gattcattca ccttttctac aagtatatcc agacctaggc aatttgtccg catggccgga
     4681 aaataatccg gctcgagaac ttgagaaagg gatcgattat atcactgcta tccacttaaa
     4741 agatacttac cctgtaacaa aagaatattc aggccagttt cgtgatgttc cttttggtag
     4801 cggttgtgtt gattttcttg gattattaag acatttgaaa cgtctagatt atgatggaac
     4861 atttctgatt gaaatgtgga gtgaaaaaag ccaagacttt cgaagagaaa tccagcaggc
     4921 aaaagactac ttaagttcta aattgaagga ggctggctat gatgtcttgt gaagaaaaaa
     4981 ttgcagcaat gaagcaacgt gtccttgaag caaatctggc attgccgcga tacggattag
     5041 tcaaattgac ttggggcaat gtgagtgaag tggaccgtga gctaggagtc atcgtcatta
     5101 agccaagcgg tgtcagatat gaatgcatgc aagcagatca aatggtggta acagatttgt
     5161 cgggaaatat aatagaagaa gatagcttaa aaccatcttc agatctcccc acacatgttg
     5221 ttttgtatca aacttttgaa gatataactg ctattactca tactcattcc acgcactccg
     5281 tcatgtgggc gcaagcagga cgcgatttgc cagcttatgg cacgactcat gctgatgctt
     5341 tttatggtaa ggttccttgt acacgccaat taacgaaaga agaagtaaga gaagcttacg
     5401 aagttcatac agggaatgtg attgtcgaga cgtttaagga aagaaaatta gatccaaacg
     5461 aagtccctgg agtacttgtc tatggacatg gaccgttcac atggggggat tcacctatga
     5521 aagcagtaga aaacagtttg atcttagatg aaatttgtct gatggccaaa gaaaacgagc
     5581 tgatcaaccc caatatatgt gaaatcccgc aatatttact agataaacat tattttcgca
     5641 aacatggaaa gtatgcatac tacgggcaat agagatgcta gggtaagggt agaaaattaa
     5701 aagataacac ttgatagaga gaaacagagg ttgtgaagaa aatttatcac aaccttttta
     5761 tttatcaaaa agtatgcatt ctaaaaggaa agaggaattt taaggaa
//
LOCUS       DACDHJ010000143         5805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDHJ010000143 DACDHJ010000000
VERSION     DACDHJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.145"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(89..1993)
                     /locus_tag="IV223_002037"
     CDS             complement(89..1993)
                     /locus_tag="IV223_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8712298.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            complement(2191..2424)
                     /locus_tag="IV223_002038"
     CDS             complement(2191..2424)
                     /locus_tag="IV223_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP8712299.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(2571..3425)
                     /locus_tag="IV223_002039"
     CDS             complement(2571..3425)
                     /locus_tag="IV223_002039"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8712300.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(3410..3748)
                     /locus_tag="IV223_002040"
     CDS             complement(3410..3748)
                     /locus_tag="IV223_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712301.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(3976..4593)
                     /locus_tag="IV223_002041"
     CDS             complement(3976..4593)
                     /locus_tag="IV223_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8712302.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            complement(4825..5397)
                     /locus_tag="IV223_002042"
     CDS             complement(4825..5397)
                     /locus_tag="IV223_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8712303.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
BASE COUNT         1652 a         1318 c          977 g         1858 t
ORIGIN      
        1 aagcggaaaa tcccagattt aaaaatctga gtttccgctt ttgtcatttc aaggatggac
       61 tattgtctca gcctcttcta atttattctt aagatatttt tactttctct gtaggtgctg
      121 atacttcttt ttgttctttc cgacctttca acttatactt cattaatctc atgccattga
      181 aaattaccac caagatacta ccttcatgaa ccaacattcc gatagacata ttcatccatt
      241 cgctgaagaa gacgctggtc aacaagacca acactactcc aactgaaatc acgatatttt
      301 gtttcatatt atttgcggtg gcttttacta atcctaatgc gtgaggcaag ttgctgaagt
      361 tcgagttcat taaaacgaca tcggatgttt caatcgctac gtccgttccg cttcccattg
      421 cgattccaat gtctgctaaa gctaaggacg gactatcgtt aacgccatct ccaacaaagg
      481 ctacaatttg accacgttgt tgaagttttc cgatataagc cgatttgtct tccggcaaca
      541 tgtagccgtg agcttcggtc aaaccaagtt cgccggcgac cacatcaacg gttccttggt
      601 tatctcctga aagaacgact agatttttca cgcccaagtc ttttaattcc tgcaaattag
      661 ctttcacacc cggacggact tgatcgcgaa tgcccatcag tacttttaat tcaccgtcga
      721 ccgctgtcaa aacgagagag ttcccttgtt gttcaaaccg tttgacatct ttttgcactt
      781 ttttgctgag agtgacattt tctttttcca tcaaggccac attcccaaca gccactctat
      841 gtccagctac acttgaaacg attccgccgc ctttcacaac ttcagttcct tcaacaggat
      901 aaaagtttgt ttctccaatt tgatttaaga ccgcttttgc taatggatga tctgattctc
      961 gttccacact ggccagatag ccaagcgttt cagcaggatc tttttcatac agttcagtcg
     1021 ctgcaacggt tgggtttccg acagttaaag tacctgtctt atcaaataca attgtatcca
     1081 cattactgaa gtcttgaatg acttcactcc cttttaaaag aacaccgtta cgagcaccat
     1141 tcccaatacc ggcaacgttt gagacaggca ctccgataac taatgcgcct gggcaaccta
     1201 gaaccaagat ggtgattgct aactcaatat tttgactgaa cgcccataca acaattgcca
     1261 gaaccaagac agctggtgtg tagtatttag aaaagcgatc aatgaaccgt tccgcttcgg
     1321 atttagaatc ttgtgcttct tccacgagtt caataatttt tccaaatgtg gtgtcctcac
     1381 caactcggtc agctctgatt tgaatcgttc cgttttctaa aatggttcct gcaaaaactt
     1441 ctgcatcagc tagcttgttg accggaatag cttcacctgt aatgctagct tcattgatat
     1501 gcccttcacc cgtaaggact atgccatcta ctggaacttt cgcacccgtt ttaacgagca
     1561 agatatcccc ttcatctata tcatccactt ctacttcttc aaattcgcca ttatccattt
     1621 gtttcaaggc gctttcgggt gccatttcag tcaattcttt gatagcagat cgcgtttggt
     1681 tcaatgttcg ttgttccaag tagtgtccga ataagaataa gaacgtgaca atagccgatt
     1741 cttcataatt ttgaataaaa agcgcaccaa tcgctgcaat actgactaag acatcaatac
     1801 tgatgacttt gactttcatt gcttgaaacg cttgaatggc aattggcgta atcccaaaga
     1861 cagaggcgat gatcaaagac cactctgaaa gtcctacgtt ttctaaaaca aagtgactga
     1921 agaaaccgag tacgattaac aatccactga tgacggtgat aacatttttc ttgcttaata
     1981 tatattgttg catgcttttt ccctccaaat ttaatttctc ttttattgat aaacacagta
     2041 taagataatt gcctaagtaa ttaattgact gacatcaagt ttagaaagtt ctcttttatc
     2101 gtttctaagc acctcgaatc ggattgcaac aaaaaaagca atcctggaaa aatagctttt
     2161 cttccagaat tgcctagttc atttaatctg ttaaacggcc ttgacttttg ctttaagaac
     2221 cggatagcct acgttttcga tagctttttg aatctcttca atcgaagtga cagctgaatc
     2281 aaaattggtt ttgactttgc tggagttgaa taatacttta atgctgtctt tatcaatacc
     2341 gttaactgat ttcacggcac cttcaatttt ttgcatacaa cttggacaag acaacgtttc
     2401 taattgcaat atagcttttt ccataattcc tatctccttt atgtgttttt attttatcgg
     2461 cagcagatta atgaacactg ccttttcctc tttacaactg tagtatagct cacttttagc
     2521 aaacgaaaat tgacgcatat caagttttgc acttcttttt atctttttgc tcaattcacc
     2581 atcttaatag tctttttctg ccccttattg ccatcagaat aataagaaat tggtggataa
     2641 tcttgtctgc gtataaacca tactacaatc gcgccaataa acagggccag agacaaggct
     2701 tgcgaaacgc ggatccactc gccaatccat aaactgtccg ttcgcatacc ttcaatgaag
     2761 aaccgaccca ctgagtacca caaaacgtaa ctcaaagcga cttctccttg acgcaaaagc
     2821 tgttttcggt tccgtatagt gacgatgagg acaaagccca acaaactcca caatgactcg
     2881 tataaaaaag tcggatggta gtaggtacca ttcatattca tttgttcaat gataaattca
     2941 ggtaaataaa gattttccaa gaattgacga gtaaccggcc caccatgagc ttcctgattc
     3001 ataaaattgc cccaacgacc aatggactgg gctaacaata cattgggcgc taaaatatca
     3061 agaacgagtg ccaaagaagt accttttttc tttgcatacc aatatagcgt accccctcct
     3121 gcaatcaatc ccccataaat cgcaatgccg ccattccaaa tcgcaaggat ttgtcctgga
     3181 ttttgcagat aataaccaag ttcaaataac acataataaa gccgagcgcc gaggagtcca
     3241 atcggaattg cccataaggc catatcgata atggtatcct cttccagtcc ttttttcttt
     3301 gcttccttgg tgctcaactg gagagccgct aatatgcccg ctgcaataat gactccatac
     3361 caatgaatca caacagaacc aatctccacc acatggggat tcaaaacgcc taatacgaat
     3421 cccattctat ttgctccatt tcttcaattt ttcgcgtcat tctagtcatg gtgatgctga
     3481 acaagattcc ggctaagctc catggaaacc aaacagtgaa tacactttta tctccctggt
     3541 cactcgcttc gtaatacgcc acttttccgt gtccgattac gccggctcct tttacaactt
     3601 cctcttcttt ttccatagct aacctatctg cctcaacaac gttagacgtt tttgcttcgc
     3661 cgccaatcgt gttcttggtg aaagggacga caaagaatgc ccccgctgct gacaacgtga
     3721 tggcaactat cacccaaaaa caatgcacat tcaccctact tatttttacg ttttgttcga
     3781 caaccctatc ttaaagaaaa ataaaacaaa aaaacttgag ggttatcatg tttgtgcttt
     3841 tatttttcca atgaatgaag gtcgttctgt cttaaaaaag ccaaaaacgg acagatggaa
     3901 gcaaaagcac tgagttctgt cccgttgtta atgaagagcg acagaacacc aacgtgtaga
     3961 aaaaagtgtg tccgattatt cgctgtaaag caataaatca tctaaatcct gaatcttgat
     4021 ccttttccca gacagctgtt gaatcaatcc ttcgtcctcc aattctccaa atttgcgact
     4081 gatggtctca ggtgtcgttc ctaaatagga agcaatgtcc ttttttgcca tcggcagggt
     4141 gatggtggga gagttgccca tttcaggttc cacattttct gccaaaaaca aaactaaacg
     4201 ggtaatgaca ctctccacgg ctacttgtgt cgtttgacgt tctgaacttt ctaaccgttt
     4261 agacatttcc gccaatagtt ttaaagaaat agctgggtat tgttccagaa aatcttggac
     4321 atctttttgc tgaatcatac agacaacggt atctcgggtt gcttcggcat attcctcgtg
     4381 tactgcatcc gggttgaaca aggtccattc tccggtgaaa tcaccagggt tcaagatccg
     4441 cactagctgc tctttgccag agtctgccaa tcgataaata cggattttcc cacgattgat
     4501 aatatataaa gtatcctctt tttcttgtga acgaaacaga aactctcctt tttgatactg
     4561 tttcgtgtgg gctttttcag caatgtaatt catcgcgcta tcctctaagt gattgaatat
     4621 tggaaccaag cggatacagg ctgcgtggtc gccaatttca tgatgggtat gtttagtcaa
     4681 ttcattctcc ccctttattt attttgttta tattcgctgc ttttattata taagtctctt
     4741 actacaaaaa aattgatgca catcaagaaa atggaaaaag gactggaaag aattccaatc
     4801 cctaaatcag cttctacttt taccttaatc ttcatcctca tcaagagcgt catcatcttc
     4861 taatgcttct ttaccaatga acgcttgcag catccaaatg ttcttgtcta agtcattttt
     4921 aaaagcaatc aatgtatctt ctaaagcatc gtcaccctcg tcttgagcta aacgaatcgc
     4981 gcgaatagtt aggtcacgag tgcttctgaa gtcctcgata atattttcaa ccatgtcttc
     5041 tgctttagcg tatttattaa ctgcatcttc cgaaagcatc gaatattttt caaattcaac
     5101 cgttgtcgaa gcaggtttat gcccagaagt aattagacgc tccgcaagtt tgtcgaacca
     5161 tttttcattt tcgttataca actcttcaaa tttttcatgc aacgtaaaga aattggctcc
     5221 ttttacatac cagtgatatt ggtgcagttt cacatgtaat gtatgcatgt tgcctaaaag
     5281 atgatccgca atggccgctg cattaatttt cgtatgatga acatgttcct tgtgttcttg
     5341 ttcttcttgc aatctttctt ggggtgttct ttcttgagta gctttatttt cagtcatgat
     5401 ttattcctcc tataattttg gtttgtttaa aatagctttc tttgattcct cctttttctt
     5461 tactgaattt agtataggat agatttccgt taaaaaaatt gacgctcatc aagaaaatta
     5521 tttcttaata gacgccaggc ttctctatta aattcaactg aaataatcaa aggaatgaag
     5581 tcgttccctc caaacaaaaa tttcattaaa gtagggccta aaatgtcatt tttatactta
     5641 attggcacag ttttttctaa ttatttgtca tattcatagt caactatagc tacaaaaaca
     5701 actaattttg gagatagatt gagatattta aaaatctctc atgaaaaatt agttgctttt
     5761 gtcacttagc gctgaaaatt tatgccattt atctagaaaa atgac
//
LOCUS       DACDHJ010000144         5660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDHJ010000144 DACDHJ010000000
VERSION     DACDHJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.146"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..213)
                     /locus_tag="IV223_002043"
     CDS             complement(<1..213)
                     /locus_tag="IV223_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712304.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIE"
     gene            complement(398..796)
                     /locus_tag="IV223_002044"
     CDS             complement(398..796)
                     /locus_tag="IV223_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8712305.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(793..1566)
                     /locus_tag="IV223_002045"
     CDS             complement(793..1566)
                     /locus_tag="IV223_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8712306.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1563..2246)
                     /locus_tag="IV223_002046"
     CDS             complement(1563..2246)
                     /locus_tag="IV223_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8712307.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2413..3300)
                     /locus_tag="IV223_002047"
     CDS             complement(2413..3300)
                     /locus_tag="IV223_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8712308.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3297..4166)
                     /locus_tag="IV223_002048"
     CDS             complement(3297..4166)
                     /locus_tag="IV223_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8712309.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4303..5589)
                     /locus_tag="IV223_002049"
     CDS             complement(4303..5589)
                     /locus_tag="IV223_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8712310.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1746 a         1260 c          932 g         1722 t
ORIGIN      
        1 ctcgatataa tcttttactg gtaattccaa ttctacctta aaaaagtgca aaacagccga
       61 cagcccttct gcttgataga tttctcctaa tgttagcccc tctagatgga actctggtga
      121 aaaatgcagc aatgttttat tttcctgcaa ttgaacatat agaaatgtag atcgctcaat
      181 cagagtatct tttaaaacaa ctaaaaaatt catttttctc cccctttttt catcattttt
      241 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      301 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      361 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      421 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      481 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      541 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      601 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      661 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      721 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      781 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      841 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      901 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      961 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
     1021 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
     1081 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1141 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1201 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1261 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1321 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1381 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1441 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1501 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1561 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1621 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1681 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1741 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1801 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1861 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1921 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1981 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     2041 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     2101 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     2161 aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa tatagcagca
     2221 atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa gtttacctta
     2281 aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt ctcaacataa
     2341 aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa ggaacgcgct
     2401 tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct tttgcaaaac
     2461 aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga tcattccata
     2521 gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg
     2581 agattgcaaa gctactaagg gccataaata attgttccag ctactcatga atgtgatgat
     2641 tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa aaatacttag
     2701 ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag atttaacatt
     2761 ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga caaccaagaa
     2821 actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata aagggatcat
     2881 caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta ggtcttttcg
     2941 tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca atgcaaacac
     3001 agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt
     3061 ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt ttcccgatgt
     3121 gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga aaatggaaat
     3181 aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg aacgaagttc
     3241 taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca tatttttcat
     3301 cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa ggaaacaata
     3361 acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact caaattatag
     3421 atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat attttgtact
     3481 tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt
     3541 tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc gtcaatagat
     3601 gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa aatcatattg
     3661 tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca aattggatga
     3721 gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt gtttacaagg
     3781 ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga agtgatacat
     3841 ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc atttaaaagg
     3901 ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa atacaggaat
     3961 gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata attacctaac
     4021 ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa agccaaaacc
     4081 atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa agcatgcccg
     4141 tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt gattatctac
     4201 atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg tcttttcgtt
     4261 tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag ccaattgtgc
     4321 ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat tgatgattcg
     4381 atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt aattcacttc
     4441 tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc
     4501 agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata ctagtccgat
     4561 ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt ctaggaaatc
     4621 tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc cgccaaggtt
     4681 agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa tcttccattt
     4741 tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag ttgggctaga
     4801 agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg tttgttcgac
     4861 tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag atgcattgtc
     4921 ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct gttgcataat
     4981 catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc ccgtcttttc
     5041 tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca tatcctcttc
     5101 tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta cgcccgaatc
     5161 gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat acggtgcaaa
     5221 atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag aagtcaaata
     5281 gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg tattcccgct
     5341 cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa caacttctat
     5401 ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga tattgaatgt
     5461 ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac tagcacttcc
     5521 gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa ttaactttct
     5581 gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg ctacattcat
     5641 cataccaaaa tttgaaaaaa
//
LOCUS       DACDHJ010000145         5634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDHJ010000145 DACDHJ010000000
VERSION     DACDHJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.147"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(214..921)
                     /locus_tag="IV223_002050"
     CDS             complement(214..921)
                     /locus_tag="IV223_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712311.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(986..1822)
                     /locus_tag="IV223_002051"
     CDS             complement(986..1822)
                     /locus_tag="IV223_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712312.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1840..3249)
                     /locus_tag="IV223_002052"
     CDS             complement(1840..3249)
                     /locus_tag="IV223_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8712313.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3263..5086)
                     /locus_tag="IV223_002053"
     CDS             complement(3263..5086)
                     /locus_tag="IV223_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8712314.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINRPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5366..5593)
                     /locus_tag="IV223_002054"
     CDS             complement(5366..5593)
                     /locus_tag="IV223_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712315.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1714 a         1232 c          937 g         1751 t
ORIGIN      
        1 cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc agtagcttat
       61 cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg taaataaatg
      121 aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt aatcagttac
      181 ctgtccgttc ttaaatacca cagaataatc tttttaccca ttttcaagta accgaagtgg
      241 aatgtcttta ccaacttgct ctgtcactgc attactgata cctgttagat tagctaataa
      301 atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg tgattccttt
      361 atcttcattt acaagcttcc aagtaggtgg tatagctgtg tatgtttcga tgaatgaatc
      421 aattctttca tctatcgtct tcccagtatc tacttctgtc tcattactct tagatgaatc
      481 gattctttca tccatcgtct gctccgtaac tgcttctgtc tcattattct caccggaaac
      541 tctcttcaca cccaaactta tatattgtcc atctttagtg tgcatagtta aaattcccac
      601 gcttttacta ataaattcac tataaccagt tctttcaata gagacttcaa tgtatttcac
      661 actatcatac tcaaaagaaa aatagacatg taaaagcatt ttgttgttta catctacata
      721 ataatccttt gactctactt gagaccttcc tatacggaat acagctttac tgtctgatat
      781 ctctatacca gcatccttat tattaatctg ccaatctcca agtagctctt ctggcaagct
      841 agtagtttct tgtgaacact ttaccacttc actggtagag gtcgaaacat tcacctccgc
      901 actgttagaa gaattttcca aggaatcagg agccttagca caaccaacaa gtctttatat
      961 attgtgcagc cgatattttc ttcattcagt acacccttga aatatgcagt gttagataaa
     1021 gcagttcgtc ctgatttgct gaggcaccca tctcttcttg aacgtactcc ttgattttca
     1081 aggccgcccg atactctttc ggaaaacgct gcttcactga agccagcaaa aaatcatcct
     1141 tctccggcaa ctcttccttc ttaatcattc gggcaataaa aaactgtaaa tgcgtcacaa
     1201 atcttaagta aagcatcgaa tctttatcaa cctttttttc gggaaacaga tatcctagca
     1261 tagccaagat atcctttagt gctcttaccg agagcatggt ttcttccata tttgcttgtt
     1321 cactttgggc atttacaatg tgaaacgcaa tgtttccagc ctcctcttct ggcaactgaa
     1381 cattcatttt tttgttgatc aattggatag catagcagcc gatctcaaat tcttttgggt
     1441 aatacttttg aatttcaatc agcaggcgat tttgaaaaac aatgcctttt tgactgcgtt
     1501 caatggcatg cttcaagtga tcaaccaagg taaaaaatag acttttattc agcttaaagt
     1561 caaacttggc gttggcataa tcgataattc gcttgacgca aataattaga tcatccggag
     1621 cagcctcaaa cagtttaatg taatcctttt ttagagcatc agaaagtgaa tctaatttaa
     1681 atactttttg gattttgtca gccggaacca aatcgccttt ctttagttga aatccaatcc
     1741 ctttccccat caaaattact tctttgcctt gcttatctaa gcaaagaatt aaactgttgt
     1801 ttaacgtttt aattactctc attagattca ccgttctctc taagctaagc catttcttgc
     1861 gattactttt tgataccaaa agaagctttc tttttttatt cgttttaatt ctttaagatt
     1921 ctcatcggtt cggtcaacat atataaaacc ataacgctta ttgaagcctt gatgggtact
     1981 aacaacatca atcgctgacc aaggtaaata accaaaaaca tctacaccat ctgttaaagc
     2041 tgcttgaata gccagaatat gtttggctag ataatcaatt cgatcctggt cattaattcg
     2101 tctatcttcc tcaactatgt cgggtgcccc atagccattt tcagtaatta aaataggtaa
     2161 atcatagcga tcgtacaatt tcctcaatgt taatcgtagt ccagtagggt caactaccca
     2221 gccatatggc gttttatcta cataaggatt ttcagctgcc cggtagacac cttcttctcc
     2281 caacatgatc tgttgatccc ccgctctagc ggaaacatct gatccgtcat ttttgctggc
     2341 agcgatggtg gcagttgaat aataattaat cgcaataaaa tcgggtttac cggaggataa
     2401 aattgcctga tcctctgcct caattttagg atataatcca cgatcattca aatagttttc
     2461 aaacaaataa ttgtattttc cccgaactgc tacatctaag aaagaccagc ctctcaatgt
     2521 ttcccaatta tgggctgcaa ttgcatcttc tggactggag gtagcttgat acatagccgt
     2581 taaattgatt gctggaccga tttttccttt aagattcaac tgatgataca agtgaattac
     2641 tctagcttga gctaataaaa catggtgatt aatttgaaag agttcttttt ttgaaggcag
     2701 ctctccactt ggtggcaaac caatcgctcc aggatgaagg atcatcgtgt tttgctcatt
     2761 aattgtcagc caatagttca ccttgtcacc atactcagta agcaatacct gtacatagtc
     2821 cacaaaataa tcgatagttg ctcgatttaa ccaaccgccc tgctcctcta gaaaataagg
     2881 taaatcgaaa tggtacatgg tgacaatcgg ttcgattccc tgattcaggc attcatcaat
     2941 tacttgatga taaaaagaaa ttcccttttg attggtttta ttattttcaa tatccataat
     3001 tcgcgtccaa gcgatggaaa agcgaaaagc ttttaagcct aattctttaa acagctgtat
     3061 atcttcttta tagtgatggt aaaaatccga ggcaacttca aaattagcta ccccttctgt
     3121 atgctgatac ttgtcaataa tagacggttg tttcccatcc tcctgatagg caccctctgc
     3181 ctggtaggca ctaatggagg caccccataa aaacttctct ggaaatgctg aaagctgttt
     3241 gtattgcaca aaaatcctct ccttaactaa tttgcatcaa tggttcgtgt tcctttaagc
     3301 ttccgcttgc ttcactaatt cctgcgtaat tttcactgtt ggtcactacc acaatcactg
     3361 tatcatcgta gccagcgttt ctaatggcag cgaaatcggc gttgattaat aattgtcctg
     3421 ctgacacctg atctcctgct tttacaaata cttcaaaggg ttcccctttt aaattcacgg
     3481 tgtcgatccc cacatggatc aataattcaa taccttcttt agaacgtaga ccaatcgcat
     3541 gtttagattc taaaatagta atgacttctc cctcaacagg tgaataaatg ctgttttctt
     3601 ctggatgaat ggctacgccc tttcccaata gtcccttaga aaagacagca tcgttcactt
     3661 cagaaatagg acacacggtt ccttttaccg gattttcaac aatgactgct tcggctattg
     3721 ccgtttgctc tgattcatca gaagtatcgt caaatccaat tagataagca atgataaagg
     3781 ttgcaacaaa ggcgattaca cacgtaataa ttgcgtgaac aatattcatt ggattttctc
     3841 caataaaggc tggtaaggct gctaagccag gggaaacaaa ggcatatctg actaaaccgc
     3901 tcaagccagc atacaaacca gcaattgcac caccaatcat ggaagcaact aatggttttt
     3961 tcagcttcaa ggttactcca tagagtgaag gctcagtaat tcccattaaa gcagtgatcc
     4021 cagtggacac agctagctgc ttcaactctt tgtttttagt tttcgcggct acagcaaagg
     4081 tagcacctga ttgagcaata ttagaagcca gcattcctgg cctattgatc atttcagaac
     4141 ccgtactgct caattgcata gtagcgattg gcgtcattgc ccaagctgta cccgtaacca
     4201 ccaaaaatgg ctgcaaacca cccatcaaaa atggaattaa ccaagctgct ttgccgttaa
     4261 tccattcagc ccccattgct ataagatcgt tcagcaacgt tccaaacggt ccaatgacaa
     4321 ccaacgctaa aggccccata attaaaaata ccagcatcgg cttagtaaag aatttaataa
     4381 acgaaggcga aattcgatcc gcaaagggct ctacatagga catgatccaa acagtaaaga
     4441 taatcggtaa aactgatccc gcataatctg cagctaatac cggaattcct agaaaagaaa
     4501 cggcatcgcc agtagaaaaa agctgtgaaa tatttggatg aagcaaaatg ccagcaacag
     4561 taatcgccaa aattgggttg acatcaaact ttatggctgc cccataggct aatagtactg
     4621 gcataaaata aaaggctgca tcagcaatag tattcaaaat gatgtaagtt tgactactgt
     4681 ctgatagtac accagtcaaa gtcaagacgg ccaaaatggc ttttaccatc ccggcacctg
     4741 ttatagctgg aatcatcggt gtaaaggtgg tagaaatgac actaatcaat cttgaaataa
     4801 tactttcttg attgtctgaa gaggggccct ttttaattga agttcccact tccttttgaa
     4861 tttccttgaa ggctgtttgg acttcattgc caatcaccac ttggtattgt ccaccgctat
     4921 ccaccacact aattacacca agtgtttttt ctaaggcttt cgtatcggct ttccttttat
     4981 ctttgagttc aaaacgcagt ctggtggcac aatgggttaa gctgaccaca ttttcttttc
     5041 ccccaacctg ttgcaataca tttcttccta cttctctgta atccattatt ctctctcctt
     5101 aaagcagtaa aaaagaccaa gtcaaaccac gccccaatca acattgaagt gttagtttgt
     5161 cttggtcttg cctgcataat cagtaacatg ccaataacgt tattttgctg taatccaata
     5221 atagcacatt atgatagcgc aatcaacagg gaatttaatt ttttatcttt tcgtttgacc
     5281 gtatagggta atataacagt tagatcacat caactagggt atatctgcgt atacttactg
     5341 ctaaaataag tatatctaat attaactata ccagtaaaac cattcctaca taaccagcgg
     5401 gaaattccca aaatcgtgaa agcatcaaaa atgcatataa cggatcacac aacttcaaca
     5461 attctgctct tgggaaaaat tgatgaattt ggtgagtaaa ggatggaata aacagcattc
     5521 caacaatata ggcaacaata gtgatacttg tttttttggt aaaaatcagt aagcaatagc
     5581 caatttcaat cattccgaga tataaaggta tcgatagaat gatttgggct agta
//
LOCUS       DACDHJ010000146         5628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDHJ010000146 DACDHJ010000000
VERSION     DACDHJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.148"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            177..1982
                     /gene="glmS"
                     /locus_tag="IV223_002055"
     CDS             177..1982
                     /gene="glmS"
                     /locus_tag="IV223_002055"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8712316.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(2054..3409)
                     /locus_tag="IV223_002056"
     CDS             complement(2054..3409)
                     /locus_tag="IV223_002056"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8712317.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3433..4602)
                     /locus_tag="IV223_002057"
     CDS             complement(3433..4602)
                     /locus_tag="IV223_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712318.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(4599..5486)
                     /locus_tag="IV223_002058"
     CDS             complement(4599..5486)
                     /locus_tag="IV223_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8712319.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
BASE COUNT         1578 a         1241 c         1084 g         1725 t
ORIGIN      
        1 ccaggtctgt gtaaacagat gacgaggaga gggtttatcg aaagattcgg cggatggccc
       61 tagggactgc actctacaga aaatccacaa aaaatatctg caaagataaa acaaaaggat
      121 tttcctgcag ctggaagcat tcatacgaat tcattataga aagtaggatg ttttttatgt
      181 gcggaattgt tggaatgatt ggattaaaaa atgtaacacc aggattgatt gacggtctag
      241 aaaaattaga ataccgtggt tatgattcag ctggtatctt tgtctcagat ggcacaactg
      301 attatctggt caaagcacaa ggcagaattc aaaatttgaa aaacaaaata aatattgata
      361 ctacaggaaa tataggtatt ggtcatacac gctgggcaac tcacgggcaa ccttctgaag
      421 aaaacgccca tccacatact tcccaaagtg gacgcttcgt gctcgttcac aatggcgtaa
      481 tcgaaaactt tgaagaactt aaaattgctt atttagcaaa tgaccacttt attggtgaga
      541 ccgatactga gattatcgct catctaattg aaactttcgc gaaggatact acaacgcaag
      601 aggcgttttt aaaagctttg cgggtcatca aaggttctta cgctttcgct ttgattgatc
      661 ggacagcacc tgatgttatt tacgtcgcta aaaataaaag tccactatta atcggactcg
      721 gcgatggatt caatgtgatt gccagcgatg caatggctat gcttgctcat acgaaggaat
      781 tcgttgagat tgaagatgaa gagatggtta ccgtcacatc agaaaaagtt attattcaaa
      841 attttgcagg tgacatcgta gaacgttctt cattcgaagc acaagtagat tcttcagata
      901 tcgagaaagg tacctatcct ttttatatgc tgaaagaaat cgacgaacag cctattatca
      961 tgcgtcgtat cgctcaaaaa tacgtaacag aagacaatcg tgtctctctt gatcaaaagc
     1021 tggtcgatac attgtctgat agcgatcgaa tctatatcgt tgcttgcgga actagttacc
     1081 atgcggggtt agcaggtaaa cagactctgg aaaaattgac acagatccct gttgaggttc
     1141 atttagccag tgaatttggc tataacacac ctcttctttc aaaaaaacca ttctttattt
     1201 tcttgagtca aagtggcgaa acagcagata gccgccaagt tctggtaaaa atcaaccgtc
     1261 tcggctaccc ttcacttacg attacaaatg ttgctggttc tactctttcg cgggaagcat
     1321 cctttacact gctattgcat gctggacccg aaatcgcagt agcatcaacg aaagcttata
     1381 ctgcacaaat agctgtacta actttattag ccaaagctat cggtgataaa aaagaatttg
     1441 aaacttctct tgtattcgat atcactcatg aattgagttt agttgctaat gcgatggaat
     1501 cggtcatcgc ccaaaaagat tatttagaag agttggcaga agtatatctc agcgatacaa
     1561 gaaatgcctt ttacattgga cgtggcgctg actacaacgt ttctcttgaa gctgctttga
     1621 aattgaaaga aatcagttat atccaggcag aaggttttgc cgctggcgaa ttgaaacatg
     1681 gaacgattgc tttgatcgaa gaaggaacac cagtgattgg aatcatcagt gaagaagtga
     1741 ctggtgcgca tactcgagga aacctgaaag aagtcgaaag ccgtggcgct aaaacactag
     1801 ttattgtctc agagggatta gaaaaggaaa gtgaccagct ggtgctacca actgtccacc
     1861 cttatctaac tactctaacc acagtcgtac cgactcaatt acttgcatat tacgcaacgc
     1921 tattacgggg attcgacgtt gacaaaccac gtaatttagc gaaatcagtg acagtagaat
     1981 aataaaagaa caaggaccag tgtcagacat ctgacactgg tccttgttct tttatttttt
     2041 atgaaaatat ctcttaatcc agtccgattt cttctttgac tactgccgcg attttatcga
     2101 catagtaatt aactttctca tcagtaggcg cttctgccat cactcgaagt aatggttctg
     2161 taccggaagg acgaactagg attcttcctt ctgatcccat ttcagcttct gcctcttcaa
     2221 tcactttttt gattgccgga acatccatcg caccgttttt atcacttact cggatattca
     2281 ctaatttttg cggatagatt gttacctctg ctgcaagctc tgataatttc ttacctgtct
     2341 gtttcatgat attcagtaac tgaattcctg aaagcatccc atctccagtt gtgttaaaat
     2401 ctaagaaaat catgtgccca gactgctcgc cgccaaagtt ataatcgttt ttgcgcattt
     2461 cttctacaac gtaacgatca ccaacttgtg taacgacatc tttcaaaccg attccttcca
     2521 ccgctttgtg gaatcctaag ttactcatga cagtcgtaac gatcgtatct tttttcaatc
     2581 gatttttttc tgccaagtat ttagcacaga taaacatgat tttatcaccg tcgacaatgt
     2641 ttccaagttc atccaccgca ataatacgat cgccatcgcc atcgaaagct agtccagcat
     2701 ctgctccttt ttcaacgacc atctcagcta aacgttctgg atgggtagag cctacgccat
     2761 cattgatatt taacccgtct ggatttgtgc ccatggtata aaaatctgtt tcaagatcgg
     2821 caaacaaacg attaacactt gttgaagttg ctccgtttgc tgcatcgaca caaatggtca
     2881 aaccggaaag gtcaccgttg atcgtttgta caaggaattg agaatatttc aataagcctt
     2941 ctggaaattc ttctacagtt cccaatcctt cagctgatgg acgaggcaat tcatccactt
     3001 cagcatctaa taatgcttca atttctagct cttggtcatc tactagttta aaaccatctg
     3061 caccgaaaaa tttgattcca ttatcttgtg ctggattatg agaagcagaa atcatgaccc
     3121 ctgcgcttgc tttttgcaaa cgtgtcagat aagcaacacc tggtgtagag atcacaccta
     3181 gctggaacac ttcgataccc accgataaaa gtcctgaaat cagcgcttct tctaacattt
     3241 gacctgagat tcttgtatcc cgccctacta acacgcgtgg gcgttttcct tcttcttcat
     3301 gctggcttag gacataacct ccgcaccgtc ccagcttaaa tgcaagttct ggcgtaagtt
     3361 ccttgttggc ttcaccgcgt acgccgtctg ttccaaaata tttacccata gttgtaaaat
     3421 tactccttta acttaatttt ctgaggaatt tgttctgctc atttgtgttg attcgcttgt
     3481 ttctgatgtt tcttctgtac ttgtggtcga gctgcttgtc ccagattccg aactggaaga
     3541 aatggttgtt gtcgatggtt ccgttcctga atcagcagat gaaatacttc tactcgtact
     3601 tgtattttct tgtgtccgac tgctcgtcga agcagagcta ctgcttgacg tttgcaacac
     3661 gggtgtaatc tccacactaa ctgattcggg cttgatactt aacccctctt ctactggtat
     3721 ttcaatttta cgagttgttc gatgattgat acctgagaca ttgatcggta cagcaatact
     3781 gtcaccgatc gatgcaagca aatcgctaga gccgcttact tctgcttcca gttttgaaaa
     3841 actgaaatca tagctttcta ccccggcagc tggtgttcct tgctgtatcc cataaagtcg
     3901 taccgacttg cttggcttga tcgaatcagc tctcactgtt acttgcactg gatcagagac
     3961 gatacttaat acttcccctg ctgcattcag cgcctgtatt gggacctcac tattaatccc
     4021 atcatctgtg accatcgaag cgtccacatc agctactact cgtttgattt ctgccaatgt
     4081 tttgtcacct gtagtgatag agactttttt aggactaacg gaagtatcat caagttcata
     4141 tccagatggt gtgaacgttg aaggaatcaa cgtttccaca tcgaaatctt ttattacctt
     4201 tttctcaacc gttactgtaa tagtcgatgg ttcgatcttt gctgtgacag cactgctcaa
     4261 attttgaatt tttagcggaa cctcatgtgt tccttcgccc agcttagtga tatctgctgt
     4321 gacgcggaac atccgtgtat cttcgtttgc ctctgcattc aactgaactc ggtttgcact
     4381 ggaaagtttg acagtcactg cattttcata tccatggata tagtagcgct ttgaatcata
     4441 agaaatcttg attggcaccc cagtaactgt ttgattatac gcttcatttc cagaaagcgt
     4501 atttggaacg gactggccat ttgcattgaa gtacagcgca atactgaaca gcaatgcaat
     4561 aaaactgtaa agaatgcttg tccgtttctc ttttgagatc atttgcgatc ccctccctta
     4621 gtcataccgt ctaggaaaga ttgaatcacg tttttcttgt tttttgtttt ctcttcagca
     4681 atcagttctc tacgcaaaat tgccaaatat tcttgctggg ataagccagc catcagttca
     4741 ttatctaatg taatgctgac atccccagtt tcttcagaga caacgatggt aatcgcgtcg
     4801 ctgacttcgc ttataccgac agctgcccgg tgtcgagtcc cgaattcttt tggaatcaac
     4861 atactttctg atagaggaag ataggcgctt gctactgcga tctttccttc ttttacaata
     4921 actgcaccgt catgcagcgg tgtattagga ataaagatat tgatcagcag ctctccagtg
     4981 atatctgcat ctaatgcaat tcctgtctca atatactcat ctaatccagt atgccgttcg
     5041 atagtaatta gcgcaccgat ttttcgcttg gacatatatt ggatggcttt gtcaaaagac
     5101 aagatcatct tctcatcttc ctgctgttcg ctttttgatt gtttgaaaag cgaactgcgt
     5161 ccaagatgtt ccagtcctct ccggatttcc ggctgaaaaa tcacgatagc agctatcacc
     5221 ccgtacgtaa tgacttggtt catcagccaa gacaaggtgt gcagaccaat gatttccgct
     5281 acgattcgga taacaataaa aatagctaca cctttcagaa gctgaatcgc ttttgtgcct
     5341 cgcagcagtt ggatcagttt gtatacaaga taccagacaa ccagaatatc aagtatgttc
     5401 accaaaaaat tcagagagaa caagtcaccg gaaaacattt gctgccaata gtccaaatta
     5461 aaaagttccc cgatttgaaa tgacataaaa agcctctctt ctcttttgag atttcctctc
     5521 tagtatatca tatgttttaa ttttagaggc tgatctgctc gtatttttct tgagaaaaac
     5581 gcataatttt caggaaaatt gcaagtatgc gttttatttt tctctgca
//
LOCUS       DACDHJ010000147         5547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDHJ010000147 DACDHJ010000000
VERSION     DACDHJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.149"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(274..939)
                     /locus_tag="IV223_002059"
     CDS             complement(274..939)
                     /locus_tag="IV223_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8712320.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1118..3100
                     /locus_tag="IV223_002060"
     CDS             1118..3100
                     /locus_tag="IV223_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8712321.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3104..3742
                     /locus_tag="IV223_002061"
     CDS             3104..3742
                     /locus_tag="IV223_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712322.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            3770..4198
                     /locus_tag="IV223_002062"
     CDS             3770..4198
                     /locus_tag="IV223_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712323.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            4655..>5547
                     /locus_tag="IV223_002063"
     CDS             4655..>5547
                     /locus_tag="IV223_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8712324.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
BASE COUNT         1894 a          852 c         1043 g         1758 t
ORIGIN      
        1 taccaatcta tattacttac tagattggtc gttaatctat tcggatcgaa tgagcaaaat
       61 atttttgtga atgtccatcc ttgattatga ttccaaaaag aatcattggg aggctgtggc
      121 gtaaatatta tactataagc cagaaatacc tatcattctg aactttttta tttaggaact
      181 ttcatctcaa ggctttttca attaatttgt acccctgttt tttggataag atcatgtgca
      241 ttgatttttt ctatctctac gttcatctcc tacttaatgc ctatctggct cctgagggct
      301 tctttttaag gcgtcgtcat agtcttggct gaggatttct tggtaatctg agggaatggc
      361 ttcccaagtg agcaggcgaa cgttcatgac gtgttttcct ttgtgtttga ttacagcatg
      421 gtttttctct ttttctacct ctccagcgtg aaaagacagc catgtaccat ggccaaagat
      481 atcattacat acccaacgtt cgatggaatt gtccacgtat cctacaggga gtgtggtata
      541 gccgatttgt ggtttcaaaa aaggattgaa tcgatcgatc gtttcatttt tgtattgata
      601 attgataaga aaaagataga ccaaacaaaa gacgagcaga aagaaagata gtgtcttttt
      661 gagtgagaag ggttgttttg atgaataaga tttcttgtta acaagaggtt catctggcaa
      721 tgttggttct attgattcgg tagctgattc aatagaagga aaatcaatcg gcagacctaa
      781 agtatgatgt aaaagtgaca gcatttcaaa gtctggcatt gtacgagaat tttcccaact
      841 agaaatcgtt ttatcggaaa caccaatttt ttcgcctaat tctttttgcg taagcttaca
      901 atttctgcgg taagttcgta ttttttctcc ggtgttcatc ttcttctcct ttcgattcta
      961 cagtatatag atttattcta catgacctat acacttgata gatacgtttt tttgatgaaa
     1021 aacggtatga ttttatcaaa gaatcattta agtaagggag agtggtgata atcaaaaaaa
     1081 gttcacagtt ttactcacaa tcatagtgac cggtatcatg agtggttttg ccttggttag
     1141 tataaataaa caggtactct atcggacaaa tgaatttttc catcaaacag atacttatac
     1201 tccgattcga ttgccacaac attttattgg gcaagaagaa tatgagcagc taatcatggt
     1261 atttgaaaat gtctcatcag aaactgagct ttcttatatg aaaaaaacag taaatgaatc
     1321 caatgaagta gatacatctt ggaacttcaa gatacagcca aagcttgaaa tgacattgta
     1381 ttatcaaggg gatgtcaaat cttctcacga gaaggtaccc tattctaata cctcctttcg
     1441 cgtaacgtcc attgaagaat cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat
     1501 gacgagagaa aaagaagcct atgaacgggt catttcacta ttcactgatc gattcaatca
     1561 agcatttcac actgattatg ccactaatat ttttgaagac tttgaaaata gctattcgta
     1621 ttcggttatt tcaaatacag acgtatataa ttatcgagtg tatttaacta ttggattgat
     1681 tcttttattc cttttgttat catccttttt cttattgatg aataaagaaa tcagtttatt
     1741 gttgaataat gacttttcat tacatcaagt attgtggtgt ttattgggga aaaagaaaat
     1801 cattattaca ttcataattg caggaatcat tggtggtttt atttttatgt attataagga
     1861 gcaatttctt tatttgttcc agatgtacgt tttcttgttc agcggaatat atggtatctc
     1921 tttgatttct cttttattag ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat
     1981 ccaatacatt ctttttacat ttggattgtt gttttctctt cttttgaccg ttgccaatgt
     2041 cgaattagca actatcgttt tttcttcttc acagttagct gtttcccatg aaccaccaaa
     2101 agaaatcgct ggtgaggact atcatgtatt ttatccagta accatcggga aaaaccacgt
     2161 ggattttatt tattccaatc gttttgcatc agcagcagat caggaattat atgaaacttt
     2221 gaataaaaac ggaagtatat tagtcaatgt atcagattac ttgaatgaag aaaatgaaca
     2281 gtttggcaga gggataaaaa taaatctgaa ttatcttaag aaatttcctt tgattgatgt
     2341 ctctggaagg ttgatccaaa taaccgaaaa agaaacgcac cctgttatat tgatccctga
     2401 acgatataga catacagatt taaaaaatga cctggcgcaa ataacagaat attatcaaga
     2461 aatcagtgaa aaagaaccga aatttctatt tataaaaaac aatcaaccca tttatacatt
     2521 tattccttct atcccctgga tcgaacatta tccggttgtt gaaatactga cattgaaaaa
     2581 cagtatgtat ttggaaagaa atattctcag cggagaaatc tatccaccgt taaagatcaa
     2641 gataggctct ttgtcgaaag aaaggttatt tgagctgatt gaagaaagtc agttacgtga
     2701 taacttgcag ttatcatttc cgtatactca gactgaagag atagctgtca aagtgttaag
     2761 cagatcattt cattacttga tacagatatt tttactcact ttcttttctt tcttcttgtt
     2821 gagctatgtc atgttatgca tatattttgt ctacaattgt cggaaaatcg ctatctttcg
     2881 ttcaagtggt tattcgctct ttgaaacgta taaagatttc ttcatgatga acctcatcaa
     2941 atggggaacg acaagtgtaa tcttcctttt tcttatagaa agagagccta aatatctttt
     3001 taatattttt ttctttagct tcattgattt tattctttca gttgttttta ttttgtctac
     3061 agaaaagaaa agtcagttat tgctgatgaa tggaggttaa caattggagc cgttgatagt
     3121 gataaaaggt gcgaaaaaaa tcattcgaaa caaaacgatt cttcatttgg atcgtttcac
     3181 tgcggatgct ggggatcttg tgactgttgt tggagaaagt ggagcaggta aaagtacgtt
     3241 attgaatata ttaggattga atgatcagtt cagttcagga caattctatt tgttcgatca
     3301 agagacgaaa caattaactg ggagagagaa gcgcttgata aaaagaaata ccatatcttt
     3361 tctttttcaa gattttgggt tgattgaata tgaaacaatc gattttaatt tagatattgg
     3421 attgaagttt tcgaatttaa atgtttcaga taagcgaaca gcaaagtatg aggcattgaa
     3481 aaaagtaaat ataaacaaga agctaagtac gcctatctac acattatctg gtggtgagaa
     3541 gcaaagaatc gcctgtgcga gagtcttatt gaagaatagc aaaatcattt tagccgatga
     3601 accgactggg tcattggata aaagaaataa ggataaagta gttgaaatct tatcgcaaca
     3661 agcttcccaa ggtacgttag ttatcgtagt cacgcatgat ccatatctga ccaaaatagg
     3721 aaacaagaag atcatgcttt aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc
     3781 agttattatt tgtgcactag ggataggttt tgttggagga aacgctatgt catctgaagt
     3841 ggaagcaaat tcagttcacg gtgaaatcta ttacaaatct ttaaagccag gagagattca
     3901 ttttgatgct aaaggtaata ttattaagtc aaatttacca gtagaccggg aaatttctag
     3961 aacagcaatt aatcatagct ctggaagatg ggtttattat agtgatacaa cttgtacgag
     4021 aaaaacaggt cattctaacc actatagtcg tgtctataat cgtcacggtg caaaagcgaa
     4081 agttggaaac tctattcgat cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc
     4141 gacaggaaat agaactgaca cattctcatg ttggtacaat ccaaccggtt ggtattaagt
     4201 atccaaaagt ttatataaaa tagaaaaaac atataatgaa acagcgttaa aagttatttt
     4261 tggtagtttt aatatattca tttttagttg tataaaaata tatcataata aaaacaacaa
     4321 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
     4381 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
     4441 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
     4501 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
     4561 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
     4621 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
     4681 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
     4741 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
     4801 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
     4861 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
     4921 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
     4981 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
     5041 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
     5101 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
     5161 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
     5221 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
     5281 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     5341 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     5401 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     5461 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     5521 tttgagtaca ttcacttcag attctag
//
LOCUS       DACDHJ010000148         5523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDHJ010000148 DACDHJ010000000
VERSION     DACDHJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.150"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1082
                     /locus_tag="IV223_002064"
     CDS             <1..1082
                     /locus_tag="IV223_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8712325.1"
                     /translation="DTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDGL
                     GSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEIK
                     TREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVTP
                     YIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRGA
                     RTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNFV
                     IDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDYL
                     NTVKLVTELVKRLDSETVEKIRSYE"
     gene            1082..1885
                     /locus_tag="IV223_002065"
     CDS             1082..1885
                     /locus_tag="IV223_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8712326.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            1955..2695
                     /locus_tag="IV223_002066"
     CDS             1955..2695
                     /locus_tag="IV223_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8712327.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2706..3710
                     /locus_tag="IV223_002067"
     CDS             2706..3710
                     /locus_tag="IV223_002067"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8712328.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3755..5518)
                     /locus_tag="IV223_002068"
     CDS             complement(3755..5518)
                     /locus_tag="IV223_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712329.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         1769 a          918 c         1129 g         1707 t
ORIGIN      
        1 tggatacaaa agaagaacag ctacttattg atttaacttc tgcaaaagga gtacctggaa
       61 atgaagaaga ggtaagagaa gtattcagag aatatgccaa accattcgct gatgatattt
      121 tttatgacgg tttaggcagt gtgattgcca aacatggtgc tggcggaggc cccaaggtct
      181 ttatttctgg acatatggac gaagttggtt ttatggtaac aaaaataacc gaaaaaggat
      241 tcttggaatt ccaaactctt ggaggttggt gggggcaagt catgcttgct cagcaagttg
      301 aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg
      361 ttttaacacc tcaagtaaga aataaacctt atgaaataaa ggatatgttc attgatattg
      421 gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg tcctggagat atggtcactc
      481 catatattga atacaaacga atgaatggca gtaaatattt actggcaaaa gcatgggata
      541 atcgtatcgg gacagctgtt tctctgcgag tattagagaa tctatctaaa gaagcacacc
      601 caaatgtttt attcgcagga agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa
      661 caagtacaca ccttgtaaat cccgatattg cttttgcttt agatactggt acagcaggag
      721 acactccagg aatgacacca aaagaagcag attcgatttt aggaaaaggc ccacagattt
      781 tgatttttga tgcatctatg atcccgcata aaaaattatt gaattttgtt attgatgtag
      841 cagaggaaat ggatattcca ttccaatata cagtgatttc tggcggagga actgatgcag
      901 gacaaatgca tttgacaaga gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt
      961 atctgcattc ccatacatca gtgattcatg aagatgatta tttgaataca gtgaaactag
     1021 tcacagaatt agtgaaacgg cttgatagtg aaacagttga aaaaattcgg agttatgaat
     1081 aatgaataat agaataccta tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa
     1141 tcaatgggga atcaatactt ggcttgtttt cttatctata atatttacga gcgtcatcca
     1201 gaactttttt gacggtttca gtccttttcc ggaagaaagc atgcagcagc acttattgag
     1261 ttttctagtc gataatttac ttttattcgc attcaactat gcattgtttt acattgcgct
     1321 agtagttgta cggggtggca gaccaaaaag taacctttta ttttcagttt tccaaagaaa
     1381 gtattttggt tcgatacttg gcatcaattt gatcaattca gtcatcaact ggctactcaa
     1441 cagtttaatc tttctgccca tgtttttaat cagcggagtg agtacttata tgcagctgct
     1501 tttcagttca aatgcggtac ctacgggtgt catgccagaa gattttcttg atatcagctt
     1561 tgttttatcg atgataggga tgatttttgt ttcagcactc atcatgagta ttgttggcgg
     1621 tttattccaa tttgctgcat ggacaaagtt cgactatcct gatttgtcgg ttattcaatg
     1681 tttgaaatat ggctggtttt tattgaaaga tagaattggt acgtatattt tgttgcagct
     1741 gtcttttatc ggctggtatt tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt
     1801 cattgtttat gtgaatgtgt cagttgcaga attttatgaa caagcacgga ttgaaaaagg
     1861 aggtccagac gcctatttta cttagcatgt aagcagttta ttcatcaaaa aaataacttt
     1921 tgtggtagga taaatctttg agggggaatg tacgatgaaa ttaactgttt taggatgctt
     1981 aggagcgtat ccttataaaa atcaagggac aacaggttat ttgcttcaat ctgacgggtt
     2041 caatttgttg atcgatgcag gtagtgctac gctgatcaaa ttacaagaac accttgatcc
     2101 acttgatcta gatgctgtga tccttagcca ttatcatcat gatcatattg ctgaccttgg
     2161 tgtattacag tattactggc aacttcatcc taagagaaat ccggagacat tgctttcaat
     2221 ttatggacac acaaaagatt ccattcactt tgaagaattg acgttggaaa atgtctcaaa
     2281 aggacatcct tattttgaag ctgaagaatt aaaaattggt ccttttctga ttacttttat
     2341 ggaaacaatc catccagtag tatgttacgc catgcgtatc gtagaagaaa caacggggaa
     2401 agtattcgta tttacaggtg attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga
     2461 tgctgatttg tttctagcag atacctatct ttttgaagga aatgaacatc atcatgccca
     2521 tttcacttca aaagaagctg gagaacttgc gaaatcagct aacgtaaaga aacttgtact
     2581 tacacaccta cctcagtttg gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc
     2641 aggcaaaggg attgatatag aacttgctgc agttgacaaa gtgtttaaca tttaggagga
     2701 agaacatgat ttttgtacca aatgaaaacc acgacccaag aatcaattta gcaattgaac
     2761 aatatttgct gcaagaaatg cctttagatg aacctatttt gctcttttat atcaatgaac
     2821 cttcaatcat catcggcaga aaccaaaata cgcttgaaga aatcaatcga gattatgtgg
     2881 aagaaaatgg cattcatgtt gtccgtcgct taagtggagg cggagctgtc taccatgatt
     2941 ttggaaacct gaactttagt tttatcatgc ctgatgatgg ggattcattc cgtgactttg
     3001 cgaaagtaac acagccaatc atccaagcac ttcatgaatt gggtgttgct ggggcggaat
     3061 tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg
     3121 ctaccaacgg tcggatgttt gctcatggaa caattatgtt cgactctgat atcaatgaag
     3181 tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc
     3241 gttctcgtgt aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa atgacaacag
     3301 aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa
     3361 aaacgtacga attgactgat gaagattgga aaaaaatcaa tcgaatctct gaggaatatt
     3421 atcgaaattg ggattggaat tacgggaagt ctccagattt caatttctct cgccaaaaac
     3481 gtttttctat cggttcgatt gaagtccatt tgaatgtctc agaaggaatg atcaaagaag
     3541 caaaaatctt tggcgatttc ttcggactag gagatatctc tgacgtggaa aaaaaattgg
     3601 ttggacagaa atatgataaa gcatctctat caaaaatagt cagccaaatt gatgtgaaga
     3661 aatattttgg tgcgatagaa ccagaagaat tgcttacctt actttattga aaataaaaaa
     3721 tagcggaatc cacatatatg gattccgcta ttttttatac aggattcggg taatttcgag
     3781 agagttgaac agtgatccga tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt
     3841 aacgatccgc tacgtctttc atcatcatta agtcaaaacg ttcctgatca tctaattctt
     3901 ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc actatgttcg caacaaatga
     3961 cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt tcgatacagt ggattttcga
     4021 tggcatgagt ttctctaaag gaatgctcta aagcgtggat caaaagttga gccagatcgt
     4081 catcgttgat aggaagttct tcgggaagat ctgcttggaa ggttatctga atattccgtt
     4141 tggcagctaa cttctggtaa cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag
     4201 aacacagcat ttcgaaattt tttgcttctt ctaatatctt ttccacatag tttttagctt
     4261 ttgtttggtc cgtatgcatt aaatcttgca gattctccat ctcttggaca aattccaggc
     4321 gaagtgtatt tacttcttgg atatgctgtc gaagctgctc ataatgttgc tccaacaaat
     4381 catttttgat ttttaagaaa tctacttgtc tccgatcttt gtcggtattt aagatgtgat
     4441 cgattagatt gtagctgata ataacgatta gaatgataat atataaaatc gtagaccaat
     4501 tgatcgttgt ttgatagata cctaatgccg tttggatgta aatcaagcaa tggataatgg
     4561 ctaccaagac gatccaaggt gtacaagcta cataaaaacg gttattctta tatgcttcgc
     4621 caatgcatgc aagtgaagtg actaaagtgc ttataacact agcaatatta gcaatcggca
     4681 ttaattcagg aagttctgca ggagtaaaag ttgcataaag cagaacgccg ataaaaatca
     4741 aaagttggaa cgtacaaaaa attccgtacc attttttata gtaaaacatt cggctccaat
     4801 agtagcagat gatgaaagct gacgttaaga taataaataa atgagataaa aaagaatgaa
     4861 cgaatggttc aaaaagaata ccacttaaaa tatcttctga tacagcttca aatcctaatg
     4921 ccaaagcaaa gcaacttaag agaataagtg agtattcaag acgtttttgt ttatacatca
     4981 agtagatcgt atagaaaaag gtccctatag cgataaaaac agcgattatc atcgtcaata
     5041 cttgcggcac tgattgtgaa taaataaaac taatcaatgg tccagttgtt cctagataaa
     5101 ctttcggtgg cagtccagca tagttctgat aaggagatga gactgtaagg gtcaattttt
     5161 ttccgatata atttgctggt aaatctatca cagccaatga gagtccggga ttgctttctt
     5221 cctgataatc tttccgttgg tataaaatct tttcgcctag atatacagta atccattgat
     5281 ggtttccttt gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa
     5341 tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa
     5401 aatcaggatc atcttttgta tagaaaaccc acgaatcttt tatttctttc atttgattgc
     5461 tgtccgcagg agaaaaaata aaataaaaga aataagcaat tcctgttaaa aataacaatg
     5521 ata
//
LOCUS       DACDHJ010000149         5513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDHJ010000149 DACDHJ010000000
VERSION     DACDHJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.151"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            408..2963
                     /locus_tag="IV223_002069"
     CDS             408..2963
                     /locus_tag="IV223_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712330.1"
                     /translation="MKVIKSKINWLSISFLILTAMIIFTSNVAEGSIFNWFDDTESQT
                     EFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLFSFVES
                     TGLNKVYQSVLNTIVIGLMVLSLMIIGYKMVTGKGTLDLKSVGMNVVMSIALILLMPT
                     LIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMINKTNDRNN
                     LTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVATKFSDNFLSTF
                     VDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLVFATDL
                     ETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNAGEGISTNIFIKTIAYICAV
                     FVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSD
                     SGEGEDRKPEKNFGETLSKKAGKTGKVLGYAHERGLSGLASDGATIASERATKPFKSM
                     RDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAK
                     VENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELI
                     NQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQETKNSNTGLEKLVK
                     ENLQNSTSSQGGRSVNVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDVRENLQ
                     SSSTSQPKTVDVRENVQNSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAK
                     KFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            2977..3174
                     /locus_tag="IV223_002070"
     CDS             2977..3174
                     /locus_tag="IV223_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712331.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3178..3789
                     /gene="mobC"
                     /locus_tag="IV223_002071"
     CDS             3178..3789
                     /gene="mobC"
                     /locus_tag="IV223_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP8712332.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            3774..5165
                     /locus_tag="IV223_002072"
     CDS             3774..5165
                     /locus_tag="IV223_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8712333.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
BASE COUNT         2084 a          782 c         1089 g         1558 t
ORIGIN      
        1 cacaacataa agcatgcgtt tttcaagcaa aaacagatca aggactattc gattacacaa
       61 agttttttct aaagggactt caaattagat ataaaaatca gcttctagaa aatatagaaa
      121 ctacagatca attcaataaa gccttcggaa ttaaagagtt acctaaaatc cctatgcata
      181 attggttaga aggagaaaaa acatgattaa aaatcgtcta atagatcaaa tatggtgtct
      241 tgatcaaaac acagatactc tcgaactaat cgagagtatc tgcttcaata ctgtagttct
      301 cgatttaaga tctgaaaatg acaaatgtgt aactcacatc ataattgaca gaaaaacagc
      361 tcagaagctt tctgagagcc taaaaaaatg ggctgaggga ggtaacaatg aaggtaatta
      421 agagtaaaat taattggttg tcgattagtt ttcttattct aacagctatg attattttta
      481 cgagtaacgt tgcagaaggc agtattttca actggtttga tgatactgag agtcaaactg
      541 agtttttaac taatccactt tatcagaatt atttgaaaaa atataacgga aatttttaca
      601 gccagttcgg tatgtggata ggctggtctg ttataaaatc aatgttcgct gttgtcgatg
      661 gcattcaaaa tatgattccc gaggttttag atttgtttag ctttgtagaa agtaccggat
      721 tgaacaaagt ctatcagtca gttttaaata caattgttat tggattgatg gtactctcac
      781 taatgattat tggatataaa atggtgactg gaaaagggac tttagactta aaatcggttg
      841 gaatgaacgt agttatgtcg attgcattga ttcttttaat gccgactttg attcacagtg
      901 gaatagagtt ttctaaaaca ttttataatg atacgactac gattacaaat tcggacgaag
      961 gcgtggcgtg gtcgctgatt gaacaagggg tcactgattt agcttatatc aataaaacag
     1021 atcagtattc aatgattaac aaaacgaatg accggaataa tctaacaaaa gaaaattttt
     1081 ggaaaacgga tcttacgcaa gtattgacag ataatgtaat tgataaaatg gaaaaagaga
     1141 actcaacagc tgataacctg cgttatgaat tagttgaaaa ttctgataat gaatttgttg
     1201 ctactaaatt ttcagataat tttttgtcaa cctttgttga tggtatcaaa tcaggatatt
     1261 atcgctatca agcgaacctg tggggaattt catttggatt gattgcttta gcggtagcgt
     1321 atgtttttag tgctttcatt ataattacag caatactcga gctagcattc aaacgtgtac
     1381 tcggagtttt agtatttgct actgacttag aaacaggaca acgatcaaaa caagttttgt
     1441 cagatatttt acagtgctat ttgacagtcg gatttcaagg attcggattg tctatgtttg
     1501 caatgttcat aaattatcta aacgctggcg aaggtatctc aacaaatatt tttataaaaa
     1561 caattgctta tatctgtgcc gtgttcgttc taataaaggg tagtggaaca gtcatgcgtt
     1621 actttggcgt ggatattggt ttaaaagaag gctatggcca gcttgcttca gcattcggca
     1681 tgggtgcaat gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa ggaagcggaa
     1741 atggaaatgg atcagacagc ggagaaggag aagatcgaaa accagaaaag aatttcggcg
     1801 aaactttatc aaagaaagct ggcaaaactg gaaaagtttt gggatatgct catgaacgtg
     1861 gattatcagg tttagcttca gatggagcta caatagctag tgaaagagca acaaaaccat
     1921 ttaaaagtat gcgtgatatg gctaatgata ctaagaataa gttcaaagaa ggtttagatg
     1981 atggtacagt gtccgctatt aataaaaata gcaaacctat gttagctaaa aataaagaag
     2041 atgaaactgg aaaatatgct gattccatgc caacacgtct ttcggataga aaagatggtg
     2101 ctaaggttga aaatgccgat aggattatgt ctagttctga acgtatgcgt gaagcaatga
     2161 aaaataatgc tgaatcgaat aataatcctg taagtgcaat tcagcaaaaa gtccaacaag
     2221 atattgaaga acgtaaaaat gctatgcacg gtcaagctaa gtctgctgaa gaattaatta
     2281 atcagaagcg acaggaagcg aaatacacgc ctgaagcaat gaatcgtgag gaaatgttaa
     2341 ggaaacgtgt agaagggcgt acaggcgcta atatggacga aaaagaagcg ttgaccaaag
     2401 aaagaatcca agaaactaaa aattcaaata ccggattaga aaaattggtc aaagaaaatc
     2461 tacaaaattc tacaagtagt caaggtggcc gatcagtaaa tgtccgagaa aatattcaag
     2521 gttcattgaa tggtcatgtc ggacggaatg ttgatgtaag agagaattta caaagttctt
     2581 caaatggtca aggtactaag acaattgatg ttcgagaaaa tctgcaaagt agcagtacaa
     2641 gtcaaccaaa aacagtagat gttcgagaaa atgtacaaaa ttctacaagt agtcaaggat
     2701 tcaaaacagt agatgttcgg gagaacgttc aaaaagatag tggagaaatg aaagaaaaaa
     2761 cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg
     2821 aaacagtgat tattgatagt gaaatccgtg aaaatgataa gggatcaaaa ccaagacgaa
     2881 ggtttaccta tgaagacaac gagttattca gagatacact taatgatcct aatccacttt
     2941 tcgatagttt acttaaaaaa taaagaggtg attgtcatgc aattaattct acagattagt
     3001 acacatgatt tcttagaact tgacgaatta tgtgaatcag taaagaaagc aacaaatgaa
     3061 aaaatcacgg tcactaatta tattgaactg atgattcaag aagaattaaa gatgaaaaat
     3121 gaaatagaga gcaatttcaa atgttgctct ctagaaaaaa atgtgagggg ataatcaatg
     3181 gatgataaac aaaaaatgta cgaaaaaaat cgcaaacata gtgaggcctt aaattttcgt
     3241 gtcgataaaa agacttatct tgaactgaca ttgattgcaa aacaaagggg aatttctaga
     3301 gcagacgttt gccgtgagcg gttaaaggga ctcaaatatg aaccgccgtt gttagctgaa
     3361 gatgataggg tagttttaac caaacagtta aatcaaattg gtaacaattt gaatcaagcg
     3421 gtaaaggcat taaatatcat tgctaaatat tttagagaag aatctgaaga taaaaaagaa
     3481 aagagcctac attttagttt tgatgctagc cgttacaaca tttttaaaga tgaacttgcg
     3541 aatagcatag caaaaaagtt gatgattagt tatccagatg acattttttt ggagtccaat
     3601 cggagtgccc cctctttttt taaattatca gaagtagaaa aagaatattt cgaatattgg
     3661 atagaaaagc aaactagacc gggtacgaaa gaagagtatt cccaaaaagt tgatcggaat
     3721 ttaattcact caattcagat tattgcaagt ttaaaagatg aggtgaaaca actttgggag
     3781 ctactataac atcgaaagga cgcaagaaaa atttatcctc gctattacaa tatgcagcta
     3841 atccttcaaa aaaagaaaaa tgtgttgcag tctcaggaat aaattgtagt gatgatagtg
     3901 aggaagcact gcaagaaatg aatttagtac gtaccaagat gaataaatct aaagatatag
     3961 cacaagcgtt tcatgttatt cattcgtttg ataagagtac ttctaaagag ttaagtattg
     4021 ataaaatgca cgaaatagct gtcgaatttg cagagaaagc ttttccaaat tcgcaaatca
     4081 ttgttgcaag tcacaatgat aaagatcatt ttcatagtca tcttgtcatt aataatatta
     4141 acatggaaac atgtaaaagg ttgcgtattg accctaaaga tttagaacta atgcgaaaaa
     4201 ttaacgatca agtcctcaat gaacatggtc ttgaatcagt gaataatgag tactatgaaa
     4261 acttagaaaa gagaaccaat ctttattcaa acgatccttc taagatgaaa tatggaaaaa
     4321 ttaattatca agagtttgtg caaaatgcga tcgaagcaac gttggaaaaa tgcgaagtga
     4381 attcttttga agatttttcg gatcatttgg caaaagaata taacattgaa gtttatcaat
     4441 tttcaaaaac aaataacaaa cttggctatg ttctttacaa acaaggtgta aatttctatg
     4501 agcgtagagg tgagttaatt actttaggaa gagataaaca atctagaaaa gaaaaaagtc
     4561 agtatgtcga acgtactttt agtgcaagaa agttagggaa aagatattca tttgaatctg
     4621 tttctgaaca acttgaaaag aatttagaag aatcacatat aaaaaatttt tttaatatgg
     4681 aaagagagca agttatgtcg ctaattagtg gagtaaatta caaaactggt gagttaaatg
     4741 taccttttgt ggaattagat tccatcgcac aaaagcaatt tagcgaccgt gaaggcgatg
     4801 aaaaattcat taaatatgat gacttcttgc gagaggtaga caagcatttt gtaaatacaa
     4861 tgtctttaga agatgatcaa gaatacacga ttaaacttca tacggatgtg aatcctcaaa
     4921 ctttacgtag tgcgaaaagt aaaacaattt ctattaaaaa gactgatcag aaagagttat
     4981 ttctaccggc aaagggtttt gaaaattgtt ctcatacacc ttctgctaaa cagattcact
     5041 atcaaatcag tcgcataatg aatgacgatg taaaaatgta tcgtgctaaa aaggattatg
     5101 aattcacaca acaaatgatt gaaaatcagc aaaaaaggca acaaaatttt gaaatggaat
     5161 tctgaatggg cttgttagtg gactttccag ttttttgcct atgaagagtt gcttggaaag
     5221 aagcagtttt taggacatac gccagctacc catctacttg ccagtcaatc cgccccgctc
     5281 acgctaggct actttctggc aagtcagatg gtgggtaaat ctctgaggga gcaagctccc
     5341 tacagactcc cttccaaaaa agaaaggtgg aagaaaaaaa tgaactatcc atattttaaa
     5401 gtctcggctt cagatgaaac taaagaaata ttcaacaatt tttacaacca aaacaaagga
     5461 ttttttggtt ccaaggcaaa tatgtttcgt gttatggttt caaatttacc agt
//
LOCUS       DACDHJ010000150         5429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDHJ010000150 DACDHJ010000000
VERSION     DACDHJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.152"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..427)
                     /locus_tag="IV223_002073"
     CDS             complement(152..427)
                     /locus_tag="IV223_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8712334.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(399..1352)
                     /locus_tag="IV223_002074"
     CDS             complement(399..1352)
                     /locus_tag="IV223_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8712335.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1964..3457
                     /locus_tag="IV223_002075"
     CDS             1964..3457
                     /locus_tag="IV223_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8712336.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3592..3888
                     /locus_tag="IV223_002076"
     CDS             3592..3888
                     /locus_tag="IV223_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8712337.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3989..4840
                     /locus_tag="IV223_002077"
     CDS             3989..4840
                     /locus_tag="IV223_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712338.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4861..5073
                     /locus_tag="IV223_002078"
     CDS             4861..5073
                     /locus_tag="IV223_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712339.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1869 a          836 c         1014 g         1710 t
ORIGIN      
        1 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
       61 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
      121 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
      181 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
      241 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
      301 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
      361 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
      421 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
      481 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
      541 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
      601 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
      661 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
      721 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
      781 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
      841 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
      901 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
      961 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     1021 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     1081 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     1141 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     1201 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     1261 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     1321 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct atactaaata
     1381 tatttagtat acttcatata cttaatatat tcgatatatc taatatactc caaattgata
     1441 agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct atattcagta
     1501 tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt
     1561 gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta caaagtagca
     1621 aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg aaataattga
     1681 ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca cgctcgtctc
     1741 aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata ccttttccga
     1801 ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaaagttt atttagtgta
     1861 cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg agattaaagc
     1921 taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga gcgaaattta
     1981 ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca ccatgaaacc
     2041 tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt
     2101 aatgaatgtt gggcgtggta tggtcattac ttcggaagaa gctattttag agaacgaaaa
     2161 gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg atgattatcg
     2221 aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga
     2281 tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag atcaagtggg
     2341 ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt ttgtactaaa
     2401 gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat
     2461 ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc taggatgtaa
     2521 ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag aaatgaacta
     2581 tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac
     2641 aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt
     2701 agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg gagaaggacg
     2761 tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt caggttatgc
     2821 attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg aaccgctatc
     2881 tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc aagcagcaag
     2941 cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa aaaataatga
     3001 tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc aaagaaaata
     3061 tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa aaagtcttgc
     3121 tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa
     3181 gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct tctacaaggt
     3241 aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt
     3301 tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag aggtatttgg
     3361 actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct tgaaccctct
     3421 taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg caaattgccc
     3481 tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc tacgtgtagg
     3541 tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg tatgtcgtta
     3601 aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt gggattatct
     3661 gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc ttgggttacc
     3721 agggaaaaaa cagttgcttc tttgccgatt gagtttcttc atgggttgtc cttagcttct
     3781 ggggagacta tggatcaggt atatgattgt ttgtgtgtgt tagagcagga gtatattgag
     3841 tataaaatag ctaacgagtt aagaaaaagg aagaagtata tccagtagta gtttttattt
     3901 acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag gaaaacgtta
     3961 aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg aacacacctg
     4021 gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat tatggccatt
     4081 aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt actcgattgg
     4141 agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag catcagacat
     4201 ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta ctcaattagt
     4261 catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc tattcgattt
     4321 aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat ctggaagaac
     4381 aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg gacacatgta
     4441 tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga aacttattaa
     4501 acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg gttatgagta
     4561 caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc acacgagtat
     4621 ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg aaatgaaagc
     4681 aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc tacgatatgg
     4741 catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat acattttttg
     4801 gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa tcgtttgaaa
     4861 atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac cttaaagaag
     4921 gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa aacgggtacg
     4981 atgaacattg aataccgaac agaatattta gaattaacga atcaaactga ttttttgttg
     5041 attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa ttgattgatg
     5101 aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct ttatttgtaa
     5161 agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg ctgtgcctat
     5221 tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag tgaaaggata
     5281 gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc ttttcgctat
     5341 cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga tgagaaaatc
     5401 gtaagagaaa ttgatcgaag atgtgacga
//
LOCUS       DACDHJ010000151         5368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDHJ010000151 DACDHJ010000000
VERSION     DACDHJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.153"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(115..481)
                     /gene="ssrA"
                     /locus_tag="IV223_002079"
     tmRNA           complement(115..481)
                     /gene="ssrA"
                     /locus_tag="IV223_002079"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(652..1425)
                     /locus_tag="IV223_002080"
     CDS             complement(652..1425)
                     /locus_tag="IV223_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8712340.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1569..1919
                     /locus_tag="IV223_002081"
     CDS             1569..1919
                     /locus_tag="IV223_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8712341.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1900..2616
                     /locus_tag="IV223_002082"
     CDS             1900..2616
                     /locus_tag="IV223_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8712342.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2616..4064
                     /locus_tag="IV223_002083"
     CDS             2616..4064
                     /locus_tag="IV223_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8712343.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4134..4643)
                     /locus_tag="IV223_002084"
     CDS             complement(4134..4643)
                     /locus_tag="IV223_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712344.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4633..5358)
                     /locus_tag="IV223_002085"
     CDS             complement(4633..5358)
                     /locus_tag="IV223_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712345.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1816 a          945 c         1069 g         1538 t
ORIGIN      
        1 atgctatttt gaatgccatt ttcattcatt taacgaaaat ctgcgaaaat aaaaaatgct
       61 caaaccctta taaaataagg atttaagcat ctagcgaaat ctgctgaaat ctcacaatgg
      121 agacggcggg agtcgaaccc gcgtccaaac acatcgccac ttaaaaatct acgttcatag
      181 tcaactcatt taaggattca ctttacagct tgccgagtga caggcattct gtatcgctag
      241 tctgatgttc tctttttcaa cttacagacg gaaagttgaa acgtatccca ctaaagttag
      301 gacccttact cgagcacatg ggcgatgccg agaggatcta cgctaactgt ttttaggcag
      361 ctaaagcgta agagttgttt tcgtttttag cagttatatt taactgtaac gtttttacgt
      421 agacgtaacc tacgaaacgc aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc
      481 cgttagctaa caagggttac gtgtattttt catacaaaat tagtatagca tatattggga
      541 taaattcaat aaaaaagcga atcatatccc attatttata taataactgc ccctttctta
      601 agaaaaaata agcgattttc ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag
      661 atcaaaatac ttcaatgcgt ggacaattcc gccttctgta tttttctttg tgataaaggt
      721 agaaagttct tttaatccgt cataggcgtt gcccatagcg attttgttgt cgcaagcttc
      781 caacaaggca aaatcattcg gaccatcgcc aaatccaaaa gtaggaactt tttccagctg
      841 aagttctttt tgcagacgtt tgacacctgc cccttttgag attcctttct tcactgtatc
      901 gatcgagtaa ggaccattcc gataaaaagt tagctcagga aaatgttcgt gatagtattc
      961 gtcacctgat tgagataaga tcagcaacat atttactgtg ttttctttaa agcctaaagg
     1021 atcaatttcc ggaacatctg aatgaatgaa gtcgtaagca ttgatcattg tttggctatg
     1081 accggtacac cagattttct gatcgttata gtaagaaagt tcatgaccgt tcaactgtac
     1141 atgatcatac attttttgac attcttctga agaaaatgtt tgagaataga tttcttcgcc
     1201 gtctactcga atgaaagaac cattcatggt gaccgcagaa gtaatgcccg catcttccat
     1261 gatagagtgg atttctgctt ctgtacgtcc tgtagcgatg acggggagaa cattgttttc
     1321 ttttaatgcg ttcatagctt ttgccacttc gggtgtgatt tttgatttcg catctagtaa
     1381 tgttccatca agatcaaaga aagtgattgc acgataattt gacacaaata aaactccttt
     1441 tttattggaa taagtttatc ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa
     1501 ttcctgaaca tattggttca gtcaacaatt tagagtatac tataagaaga gaatttagga
     1561 gggaaagaat gatttttttc agtattggta tcttattctt aataattggc agtctttttc
     1621 ggattctgcc ctcaaaagga aatctgcctt tttatgggta tcattcgcct cttgccgcaa
     1681 aaacagacgc tcattggcgg ctggctcaaa agacaagcgg aaactggttt tttcttatgg
     1741 gcttactgat ggcattgatc ggctattatt taaagacatc tgggcatacg aattattttc
     1801 tgattgaaat gcttctgcta gtatttccaa ttatgcctat ttttattatg acagaaaaga
     1861 aattacagaa atatgacctg gaaactggag ggaacgataa tgaatatttt aatgattgaa
     1921 gataatcaat ctgtttctga aatgatgcag atgtttttct taaatgaagg ttgggaagca
     1981 actttcaagt atgatggaaa agaagggctc gatgcctttt tagaaaaccc acaacattgg
     2041 gatatgatta cactagacct gaatttgccc acactggacg gtatgctagt aggaagagag
     2101 atccgcaaag tttcaagtac tgtgccgatt atcatgctga cagctagaga ttctgaaagc
     2161 gatcaggtga tcggcttaga gatgggggca gatgattatg tcacaaaacc tttcagtcct
     2221 ctgacgctta ttgctcgaat gaaagctttg catcgccgta gtgagttggc agaagcaaga
     2281 gaagaagcag tagtttcaga tgatcatttt gatatcgaga ctagtcattt caagatgaat
     2341 acgaaaacac gagaagcgta tctggacaac cggccaattg aaggactgac gcctaaagag
     2401 ttcgatctat tgtttacttt agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg
     2461 cttgaattag tctgggatta tcagtacttt ggtgatgaac ggacagtgga cgcccatatc
     2521 aagaaattac gacaaaaaat cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga
     2581 gtcggctaca aattcgatga ttctggtgtt gcctaatgcg ttatttgtat cagcaactct
     2641 tagctttctg cggaatggtc gcgttgatct tattgatcgt tggagtttct tttacacaac
     2701 tgacaaaaca aacgattgaa gaaaacaatt accagcaact gtttggatat gctgaatcag
     2761 ttgaaaagac aacgcaaacc tatgccgata cgctcccgca gtttagtcaa gatcaggcgt
     2821 ttcaaaatgc attgctgctg acagagcaag tactgacaga acaaaatgtg aactttatat
     2881 ttatcgataa aaacgaacgt gtcatttatc cgactaatac agctttactg aattttacct
     2941 taacagctga tcaatggaaa aatctgagga atggacgtca agaaaaattc acatcgaata
     3001 aaaatatttt aggacaagca caggcgacct cttacgcact tgtgccattt aatttgaatc
     3061 atgaattcta tggagcactt gtagttagtc aacctgcgcg aaatatcgat aacagcgttc
     3121 gtccaattac actgaacctg ttcaaaggat ttattttttc cagtatcgtt gcagttattg
     3181 ccagctatgc ttttgcagca ttccaagtta agcgtatcaa tcggttaagg aatgcgacga
     3241 aagaagtgac gaatggtaat tttgatgtac agctccccgt acatgacaaa gatgaatttg
     3301 atgagttagc agatgatttc aataaaatga cgaattcatt gaaagaatct caagcagaaa
     3361 ttgaagaaca agaaaatcgt cgaagacaat ttatggcaga tgcttctcac gagatgagaa
     3421 cacctcttac gacaatcaac ggacttctgg aagggttaga atacaatgca atccctgaaa
     3481 atcagcgaga aaacgcgatt aaattgatga aaaatgaaac agagcgtctg atccgtttgg
     3541 tgaatgaaaa cttggattat gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa
     3601 aattcaatgg aacagaaaca ttgaaaaata ttgttgcaca gttagaagct aaagcagaag
     3661 cagcaggaga tacattgaca ttgaaagccg ttgatgatat tgatgtttac gctgattatg
     3721 accgatttgt ccaaatcatg gtcaatatta ttcaaaacgc gatacagttt actgaaaacg
     3781 gccaaatcac gattacttta gaaaaaggct atcttgaaac gatcatcacg attgaagata
     3841 cgggaattgg aatgtctgaa caacaaatga aaagtatctg ggatcgttat tataaagtag
     3901 atccttcaag aaaaaataca aaatatggtg aatcaggatt aggtttacca atcgtgcagc
     3961 aacttgtacg cttgcataaa ggaaaattag aagtagaaag cgaactagga aaaggaacaa
     4021 aattcgctgt tcgtttgcct gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa
     4081 aagatcatga atcgatcttc tataccgctt gtttttacta taaaatgatt gatttagccc
     4141 gcgggctcta aacgatcaat ataaggactt tgagcaatag tagcatcatg agtaaagagt
     4201 aagatacctt gattttcttc atgagctaaa taagaaagca agtggagtag ctcttggatc
     4261 tccaatacac taagttcatc aaatgtatct tcaataacaa tattgtttcg cttggaaagc
     4321 aaggcatgaa gtaattgcag ctttactttt tcaaaagacg tcaactcagc tgccgcacaa
     4381 ttaagcaagg aaggagagat atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt
     4441 tctttttccg gtacatccaa caggacatgg tctttaagat tcagataagg aaacatcggc
     4501 caattttttt ggatgatcgc aaaattatcc agttttgcaa caagaacctc ttttttcaag
     4561 tgctccaata ttttatgctg tttttcgaga ggacacaaaa taacggtcaa gtgttcagca
     4621 tgaatcaaat attcaggttt cattacgtcc aggcctccta ctttgcaaat gatggataaa
     4681 aaaagtgaaa gggaatagac taagaatgag acagcatcca ctgagcccaa taagagtttg
     4741 ggcaaatccc ttaagtatat ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa
     4801 agtctgttga tccatcggaa ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc
     4861 tggcagttca gtagtcttta agacaagaaa attgatcctt tgaagaagcg cttcataaac
     4921 tgactggaag cttacgatcc acaataaaac caagatacaa caaagaaaga gaggaaagaa
     4981 cagctgccag aaaataaaaa ggaataattt ggttttagaa agaccagtca ggtgccaatt
     5041 gatcaattcc tgcttgtttt gcctgactga atagtaaaag aaaaagataa aaaggaaggc
     5101 aaatagaaaa aataaactga atatgccttt tatataaaat cggctagcta attctaaagc
     5161 cggaagaagc tctgtagatg cagaagcgcc aataaatgaa agccgatctt ggacttgatt
     5221 ggctaaagcc gattgcaaat ggtaaagagt caggcatgct atcaataaaa aagcagaaaa
     5281 gcttgaaaat aaacaataga gtattgtctg ctttttgtaa taaattaatc gagcagtagc
     5341 atagcggatt attttcatac ttttcacc
//
LOCUS       DACDHJ010000152         5312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDHJ010000152 DACDHJ010000000
VERSION     DACDHJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.154"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(115..1050)
                     /gene="whiA"
                     /locus_tag="IV223_002086"
     CDS             complement(115..1050)
                     /gene="whiA"
                     /locus_tag="IV223_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8712346.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1084..2082)
                     /locus_tag="IV223_002087"
     CDS             complement(1084..2082)
                     /locus_tag="IV223_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8712347.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2079..2963)
                     /gene="rapZ"
                     /locus_tag="IV223_002088"
     CDS             complement(2079..2963)
                     /gene="rapZ"
                     /locus_tag="IV223_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8712348.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3098..3829)
                     /locus_tag="IV223_002089"
     CDS             complement(3098..3829)
                     /locus_tag="IV223_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8712349.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(3833..5098)
                     /locus_tag="IV223_002090"
     CDS             complement(3833..5098)
                     /locus_tag="IV223_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8712350.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         1509 a         1148 c          889 g         1766 t
ORIGIN      
        1 atgttactcc agtaaagatg gaattatagt ttgacggaag aaaagcaagt ataactttta
       61 aaaaaaactg atacattcgc atctcctgct tcatgtatca gttttttctt tggattaaac
      121 tgcttcttca cgcagttttt ccgcaaattc atttattttg cgaattcggt gattaattcc
      181 cgattttgat atcgcacctg agggaatcat ttcccctaat tctttcaagc tgacctctgg
      241 atgttgaagc cgaagttctg cgatttcctg aagtttttca ggaagggcat gtagaccgac
      301 ctttgcttcg atcaattcga tgttttcgat ttgtttagaa gccgcatcaa tcgtcttgtt
      361 cagatttgct gtctcacaat tgactagacg attgaccgaa tttctcatat cccgtacgat
      421 tcgaacatct tcaaatcgga gcattgagtt ggttgcacca atcaatgtta agaaatcagc
      481 aattttttct gctcctttta aataagaaat atagccattg cgccgttcaa gcgttcgagc
      541 atgtagaccg taataattta acatatcgca aatgtcttgg ttatgctgtt cataaataga
      601 aaatatttcc aaatgataac ggctcgtttc cggattattg actgaacctg aggccataaa
      661 agcaccacgt aagtaagaac gcattttttg cgaatttccc ataatctcgt ccgaaacgtg
      721 tccattgaac atcataccat ccatgatatc aagatccatc aacacatctt ttgtgccttg
      781 cttcaaccgg acgatataga cattgttctt ttttaatttc atttttttac gaacgagtag
      841 ctcacttcgt acctgataat gatctttcaa aagagaatag atacgtctgg caattgcagc
      901 attttctgtt tggacgttca aaataaactg ctggttcaca atacctaaag agccgttcat
      961 tcgcagcaaa gcagctaatt ccgctttcgc atgttctcga tgaacttcca aaccagttag
     1021 ttctttcttc acatctgcag caaatgacat cctattctcc tcctttctcc aacaataaat
     1081 tgcttaatat tttgaaccga aaactaaacg gaacaattct tctactactt tgtctccatc
     1141 gtggaagaca ccaccatctt tcaattccaa aaagtcggtg gaaatcactc gacatccttc
     1201 atttctcaat ccgttgaagt cgtgtgcgac ttgtactaga tactcatcat agatctctgg
     1261 gtccatataa tcttcaggca ctttttccgt attgaccaat actgtatcga taaaaggctg
     1321 attcaaatgc ttgtgtaaaa cacgtatatg atcggcatct gtgaaatgtt ccgtttcccc
     1381 tttttgggtc atgatattac agatatagac ggtttcagca gttgtcttga gcattgcttc
     1441 gccgatttct ggtatcacta aatttggaag gatactggtg aataaacttc cgggtcccaa
     1501 aacgaccatg tcggcgtcca taattgctga aacgactttt cgagccgctt tggcttcact
     1561 tttatcattt gtgttgcaga catacacacg gtcaatggtt ttacggtcga tcgcgatttt
     1621 agattctcct actgcagtcg ttccgtcttt aaagacagca tgcaaggtca aaggagtttc
     1681 tgaagaagga taaacatgtc catctacatg catcattttt gctaataatt gaatcgcctc
     1741 ataagtgctt ccccgcatct cggaaactgc ggcaatgatc agattaccga tcgtatgatt
     1801 ggctaaaaac tgatcttcct ttttgaaacg gtattggaaa atatcttcat agaattgtgg
     1861 catatccgat aacgccacta agacatttct caagtcacca ggaggagcca tagcgatcga
     1921 ttctctgatt gcaccgctgc ttcccccatc atctgcaaca gtcactactg ctgtgatatc
     1981 tgctccttga tttcgcaaac ttttcaggat aactggcaga ccagtgccac cgccgaccac
     2041 tacgatcttt ggttttctta tccggtaggt tttcattttc acgaccggtt caccgtctct
     2101 ttccttttat ccttgtcccg atgtgtcaca ttgactttat agtttttccc taaagcattt
     2161 gccaaacgtt cagtcaatgc cacagaacgg tgttgaccgc ctgtacagcc gattgcaact
     2221 gtcaagctgc tttttccttc tttaatgtat ccaggcaata cagtttccag aagatccaaa
     2281 aatttatggt agaatgattg cgtttcatca aaggacatca cataatcata gactggctga
     2341 tcttttccgg tcaatggtct tagttccggg atataatgag ggttgggcaa aaaacgcaca
     2401 tccatcacaa tatccgaatc gatcggcaac ccgtatttaa aaccaaatga tacaaattcg
     2461 atccgaaatc cttggtctcc ctttgatttg aatgactcat tgattttttc acgtaattgt
     2521 cgtggtgtca atgtcgtcgt atcaatgatc acggaagctt gggctttcaa gtcgtctagt
     2581 atcgcccgtt ctttacgtat cccttcagtg atcaaaccgt ccatcgccat tggatgtgct
     2641 cgtcttgttt ctttgtagcg agccactagt tcttcgtctg aagcatctaa aaataagata
     2701 gaagtgtcga tgaagttcgt attttcgatt tcgatcagca tgttttggat ttcttcaaag
     2761 aaggaacgag aacgtaaatc cactcctaaa gccatttttg tgactttgcc tgattctttg
     2821 attaattccc agaattttgg aatcaatcga ggcggcatat tatcgataca aaaatagccc
     2881 atatcttcaa aactttggat agctactgtt ttccctgcac cgctcatacc tgttatgatg
     2941 actaattgca aattatctgt cataattacg aactcctctc acattgtgca tagtaaaatt
     3001 atatcattcc gggcgtatca ttaccagttt tctttttctt ttacaaaaag atttggacca
     3061 aatatctttt aggatatttg ccccaaatcc aattttttta cttttccgat gctgtatcca
     3121 atgccttgct tctttcagct agcgctcgtt caatcgtccg atctgctaaa atagattggg
     3181 catcgatcac tggataatgg ttatcttcag caaattcatg catcaatgac aattctgtgc
     3241 aacctaagat aactttttca cagtttaggc gctctaccgc ttcttctaag atttcgtaat
     3301 atagctcttg attcaaataa tccgattcct tgatctcatg atagatcaga taattgattt
     3361 tttcttgaag aacagtatca ggaatcactg tttcgaatcc aagatttttg acttcgcgtt
     3421 catagattcc agctttcata cttccttctg ttcctagaat cgctactctc cctgtcgtat
     3481 gctgacggac aagttcattt gctgcttctc ttggcatatg aaggatagga atatccgttg
     3541 ccgcttgtaa ttcttcaaaa aagtagtgtg cggtgttaca agtcaacaca atgaagttcg
     3601 gctgcaataa attttgtttc tcaatatcat ccagcagaaa cggcattgga ttttcttctg
     3661 atcgatctaa aatataagct gtacgatcag gaactgttgc atgattgaat aagacataat
     3721 tcaaatattc ttgatctttt gtagctttcg ttcggtggtt gatcaagcga acaaaacttt
     3781 ctgtcgccat cgtgcccatt ccgcctaaaa tactgaaaaa attctccatg aactagcctt
     3841 ctttcacatg aaaatacttt ttgaatcttg gaatatagtt atggaacata tatttcatca
     3901 acaaccagcg cttgattgac cggtcttttt cataaaacac agtagtaccg taacgatttt
     3961 ctttgatcat ttgctcagct agttttttgt cttcattttc ccgagcatat tctaaaaaga
     4021 tttttttggg cacacccatc cataatcttg ctttatctga ttgattcgtt ccataggtcg
     4081 tttcaacaaa gggtttgtta aacactcgat cttctgtgac gaaacgagcc agattcaagc
     4141 cgttcaatgt cacaaagaaa ctgctacgac cttgtctcaa attgatttca aataatttat
     4201 attctccatc tctaggatca tacttcatat cgaaattggc aaagcctgta tattcgattt
     4261 tttccaaaaa tgccttaatg gtttgataga ttttttcgtt ataatctggc atgatcacaa
     4321 cgtaattacc aatcgatgct ggtgtaggat cttctaacag tggatgacca agacacatca
     4381 tccgaacttg atgatcttca tctacatacg catttagcac acgcatatta ctatcatcac
     4441 ccgggataaa atcttgtaca atcatttcac ttttataacc ggcttcgtat atccggccta
     4501 aaattaaatc gaactcttct ctagtctcta aaataaaagc tttttttctt ccttcaaatt
     4561 ggacagaaag atactccaca ctgtttgcag gtttcaaagc aactgggaat tcaaaaggca
     4621 attcctgttc caaatgtcca tttactaaca tttcttctct gacgatcaat gttttaggat
     4681 acggaagatc atattcttca caaacttcat aaaagcttac tttattgatc agtctttcaa
     4741 ataaagaata atcaatatat ggacagataa aatattctga cagctcttgt ttatgttggg
     4801 agatcaattc tgcatatcca tcaccacaag caatcaaaag atatttttta ctcttatcag
     4861 aatatctttc ttttgccaaa cgaagcatcg tctcgataaa cacagggtct ttatcaaatc
     4921 ctggaataac ttctacattg acaattttac tgtagcgggt aggggctaac tgatccgaag
     4981 catatgcttg gcagattttg ccgtatgctt cattaaaaga acgtgccata ccataaacgt
     5041 tcatgtcgct tcctaataat atagggataa attcttcatt ttcaatactg ttcatcatta
     5101 tctctcactt actcataatc tcttgttttt cttttccaac tacacaagga atatcatacc
     5161 ataaagaaaa aagcaatcaa aggaaactcc tccgattgct caattctttt ttgaacggat
     5221 tgaacttata ggaagttttt gtctatacca atcagtgtag aaagaactac catgtaatga
     5281 ttctagaaca ctgatattaa taaaacaagc aa
//
LOCUS       DACDHJ010000153         5223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDHJ010000153 DACDHJ010000000
VERSION     DACDHJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.156"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            233..1374
                     /locus_tag="IV223_002091"
                     /pseudo
     CDS             233..1374
                     /locus_tag="IV223_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1500..2579)
                     /locus_tag="IV223_002092"
     CDS             complement(1500..2579)
                     /locus_tag="IV223_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8712351.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2682..3122)
                     /locus_tag="IV223_002093"
     CDS             complement(2682..3122)
                     /locus_tag="IV223_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8712352.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3179..3589)
                     /locus_tag="IV223_002094"
     CDS             complement(3179..3589)
                     /locus_tag="IV223_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8712353.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3769..4203)
                     /locus_tag="IV223_002095"
     CDS             complement(3769..4203)
                     /locus_tag="IV223_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8712354.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4303..5151)
                     /locus_tag="IV223_002096"
     CDS             complement(4303..5151)
                     /locus_tag="IV223_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8712355.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1898 a          993 c          867 g         1465 t
ORIGIN      
        1 gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagataaacg
       61 ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact
      121 tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga
      181 atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata
      241 ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac
      301 agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg ccaacgtcaa
      361 tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa
      421 aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag cacctatact
      481 acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct atgacgagaa
      541 cactttgact atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga
      601 cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca
      661 tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg actcaggagg
      721 agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc
      781 tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac
      841 agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg gagaaaaacc
      901 agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
      961 agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc
     1021 agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa
     1081 caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct
     1141 aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc
     1201 tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa aaataaacag
     1261 ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat cattctatta
     1321 agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct ctaacatttg
     1381 aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat gaccccaaaa
     1441 taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc atatttattt
     1501 taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt caatacatct
     1561 aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg
     1621 ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct ggacatcaac
     1681 ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat caggatataa
     1741 acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat acaagaaatg
     1801 ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa actcagcagc
     1861 tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa aagagcgaca
     1921 acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc aaaatagtaa
     1981 atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa tacaattgtt
     2041 tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa cgccactgcc
     2101 atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg aagctgttca
     2161 aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc tgcatctttt
     2221 ggctgatgct cgtaaaaatt aataacggac tggatcatgt caaatgtttg atgaataagc
     2281 atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt ataaatcatg
     2341 atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac aagatgaatc
     2401 atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct aactaagaaa
     2461 agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt cgccaaaaaa
     2521 cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac gttattcatt
     2581 atatgatgga atatgaaaat aagaaagagc aaaaagaaaa accgctaaat gacgttatct
     2641 tatgagttgg ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct
     2701 tgatgaaaat tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt
     2761 tcaagtggaa gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca
     2821 gcaccttgag cagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga
     2881 ttaatataat ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga
     2941 cacaaatcat ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc
     3001 acaagatact gggtataggt aagattcagc ggcttgagga ggggagtgta gtgtctaatg
     3061 atttctttag aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc
     3121 atttttccac ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct
     3181 attcactaac tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac
     3241 acactttatg cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag
     3301 atccttcaat ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt
     3361 gtgcactgac agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac
     3421 tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga
     3481 cgcgtttacc aggagctgca atattcaatt cgaatgtacg atcgggatta tgcacttctc
     3541 cagagcgtcc gcctgtgttg atcattttcg tagaatacat ttttttcata aaattaccac
     3601 ctttaattat attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat
     3661 ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca
     3721 aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt acatatattt
     3781 ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat gaagcatcca
     3841 taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt
     3901 catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc
     3961 agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc
     4021 aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa aaatgacttc
     4081 attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg
     4141 atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg ttcctttatg
     4201 cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagct
     4261 aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata
     4321 agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc
     4381 gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa
     4441 aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc
     4501 aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac
     4561 agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat
     4621 agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa gagaaacaac
     4681 gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat
     4741 gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc
     4801 taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt
     4861 ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa
     4921 gggtttttta cctaaaaata gaagagctag taccaacata acgatattca acactaaaat
     4981 aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac cgctgacacc
     5041 tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag
     5101 ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca tttattcact
     5161 tccttaattc attaataata ctcttatctt attataactt acttttcacc ttaagttaac
     5221 ttt
//
LOCUS       DACDHJ010000154         5173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDHJ010000154 DACDHJ010000000
VERSION     DACDHJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.157"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            484..1452
                     /locus_tag="IV223_002097"
     CDS             484..1452
                     /locus_tag="IV223_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8712356.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            1470..2375
                     /locus_tag="IV223_002098"
     CDS             1470..2375
                     /locus_tag="IV223_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8712357.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            2372..3178
                     /locus_tag="IV223_002099"
     CDS             2372..3178
                     /locus_tag="IV223_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8712358.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            3335..4303
                     /locus_tag="IV223_002100"
     CDS             3335..4303
                     /locus_tag="IV223_002100"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8712359.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(4620..5144)
                     /locus_tag="IV223_002101"
     CDS             complement(4620..5144)
                     /locus_tag="IV223_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8712360.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
BASE COUNT         1577 a         1093 c         1035 g         1468 t
ORIGIN      
        1 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
       61 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
      121 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
      181 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
      241 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
      301 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
      361 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
      421 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
      481 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
      541 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
      601 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
      661 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
      721 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
      781 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
      841 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
      901 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
      961 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
     1021 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
     1081 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
     1141 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
     1201 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
     1261 ggtctagcaa cagtaggaat cgactacgaa agcttgggta aacaaacagg gatcatggca
     1321 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
     1381 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
     1441 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
     1501 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
     1561 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
     1621 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
     1681 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
     1741 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
     1801 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
     1861 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
     1921 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
     1981 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
     2041 ctaatggatg cattgctctt tctggctcct tgcttgcgca aaacaacggc tttgctgatt
     2101 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
     2161 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
     2221 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
     2281 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
     2341 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
     2401 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
     2461 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
     2521 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
     2581 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
     2641 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
     2701 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
     2761 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
     2821 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
     2881 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
     2941 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
     3001 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
     3061 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
     3121 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
     3181 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
     3241 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
     3301 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
     3361 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
     3421 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
     3481 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
     3541 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
     3601 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
     3661 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
     3721 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
     3781 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
     3841 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
     3901 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
     3961 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
     4021 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
     4081 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
     4141 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
     4201 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
     4261 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
     4321 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
     4381 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
     4441 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
     4501 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
     4561 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
     4621 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
     4681 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
     4741 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
     4801 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
     4861 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
     4921 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
     4981 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
     5041 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
     5101 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
     5161 tatttatcct act
//
LOCUS       DACDHJ010000155         5149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDHJ010000155 DACDHJ010000000
VERSION     DACDHJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.158"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..1219
                     /locus_tag="IV223_002102"
     CDS             56..1219
                     /locus_tag="IV223_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712361.1"
                     /translation="MDQFNELLSYVFQVTEADIEESGFENKRAFIRSKKPILEVSKVF
                     GWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPEYANHGLMQDLIIVALDNMR
                     KNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLPKQIPVSGMVERLPVDHPDV
                     FSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVYYDSNHEPRGVLFYWVAEEI
                     FHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNEPLAFLIEDSQIKEQIEPYF
                     MARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVFALTWDEQGQVRVLNEPIGK
                     SVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLERIIPNMEAYFSDYF"
     gene            1239..1733
                     /locus_tag="IV223_002103"
     CDS             1239..1733
                     /locus_tag="IV223_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8712362.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            1726..2397
                     /locus_tag="IV223_002104"
     CDS             1726..2397
                     /locus_tag="IV223_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712363.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(2548..>5149)
                     /gene="clpB"
                     /locus_tag="IV223_002105"
     CDS             complement(2548..>5149)
                     /gene="clpB"
                     /locus_tag="IV223_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8712364.1"
                     /translation="EKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHFGRN
                     FYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESFGDE
                     FLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQYKAL
                     EKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRILLFI
                     DEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERRFQK
                     VLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKAIDL
                     IDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEELAEL
                     REEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTIPQL
                     EKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLNETL
                     HKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAENLFD
                     SEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEIEKA
                     HPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPEAVA
                     EQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILLTIS
                     DEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDGQLH
                     FKTEELEEIV"
BASE COUNT         1417 a         1029 c          992 g         1711 t
ORIGIN      
        1 ggatcaaaaa gattttagag aaaatcttga attaaaaccg gtcactcaag aacatatcga
       61 tcaattcaat gaattgcttt cctatgtgtt ccaagtgact gaagcagaca tcgaggaaag
      121 cgggtttgag aataaacgag cttttatccg ttccaaaaaa ccgattttgg aagtatcgaa
      181 agtgttcgga tggttccacg aaaataaact gatctctcaa atcgctatct atccttgtga
      241 agtcaatatt cacggtgcac gttataaaat gggtggtgtg actggagttg gaacttatcc
      301 agaatatgct aaccatggat tgatgcaaga tctaattatc gttgcattgg ataatatgcg
      361 caagaataag caatggatct cttatttgta tccttacagt atcccctact atcggcgtaa
      421 aggatgggaa atcatgtctg ataaactttc ctttaaaatc agagatacac agctacctaa
      481 acaaatccca gtaagtggta tggttgagcg attaccagtt gatcatccag acgtattttc
      541 cgtttatgac aaatttgccc aacaaaacca tggtgcttta ttccgcagtg attttcattg
      601 ggaagagtat tggcgtttcg aaaatgaaga tgagcgcaca gccgctgtct actacgatag
      661 caaccatgaa ccaagaggtg ttctgttcta ctgggtagct gaagaaatat ttcacgtaaa
      721 agaaatgttc tatcttgatc aagaagctcg aaatggctta tggaacttta tttccgcaca
      781 tttttccatg atctactggg tacatggaga tatttataaa aatgagcctt tagcattttt
      841 gattgaagat agtcagatca aagagcagat cgaaccatat tttatggctc ggatcgttga
      901 tgtaaaagaa ttccttcaac ggtttccgtt tgttggaaca gctgatgctt ttcatttcat
      961 cattgaagat cctgtcgctc cttggaataa tggtgtcttc gctttaacat gggatgaaca
     1021 gggacaagtc cgtgtcttaa atgaacctat aggaaaatcc gttcgactgg atatccaaac
     1081 attgacttgc ttgatgatga attatcgccg tgcttcttat ttggcaagaa tcgaacggtt
     1141 agaaacagat gaagaaacac tgaagtcact agagcgtatc attccaaaca tggaagctta
     1201 ctttagcgat tatttttgat aaaaaggatg atgtttagat gaaaatttat tttgccgcac
     1261 caatgtttgc taaaagtgat ttgttatata accaacagtt agttaaagaa atcagagagc
     1321 tctcttctga gctttcgatc tatcttcctc aagaaaatga agcaatcaat gacaaaactg
     1381 cttatgcaga cagtcgaatg attgctcttg ctgacacaga aaaagtgttg gctagtgatt
     1441 taatgattgc tttactagat gggctgacga tcgatgccgg ggtagcatcg gaaatcggtg
     1501 tggcttatgc gaaaggcatc ccagtgatcg gcttatatac tgatactcga cagcaaggcg
     1561 gaacccaccc gaaaaaaatc gctgcactac aagaaacagc tgaaaatcaa tttcactacc
     1621 tgaacttata tacaataggt ttgatcaaat tgaacggaaa agtggtttct tcagaaattg
     1681 agttgctttc agaagtaaaa agatttttag atggagggac tttcagtgat taaagaaatc
     1741 aaaaaggtgc aaatagcttt acttgctttt ggtgtattcg ttgtctgcta taatttttat
     1801 gaatttatca cacaaaaata ttcgacttct caaggaatca catttatcgt ggaatcttta
     1861 ttagggattg cattgatttt catgccacaa gtcattttga aagttttcaa acttaaaata
     1921 ccagcagcaa tcgttttgtt ttactggttt ttcttgttca tctctgtctt tttaggcaca
     1981 gggatgcatt tgatcagtat catctctttt tgggacaaaa tcttacatgc ggtgagtcca
     2041 atggtcctga cagctcttgg ctatggattg attggttact tgatgaaaga tgctgagata
     2101 tcaaaaacca gtccttggtt gttcctctta ttcggctttg cgtttgctgg actttgcggc
     2161 gtgttttggg agttctggga attcttatgc gaccagttct taggcatgaa tcttcaacgt
     2221 tttgctgctt ctgatggcac actgtttgtc ggacgagcag cattgatgga tacgatgggc
     2281 gacttgctga caaatacgat aggtgctgcc ttaatgggat tgtttgcatg gtctcaaagt
     2341 aaaaaagatg agcgttactt tgaaagttat aaattagaaa aagtgaaaaa tacctaataa
     2401 aagaggttgt gacagaagcg ggcagcttca agaaatttcg gcttattttt cgaagaaact
     2461 acttctggaa cactgtttat ttgaaaaaag gaggtctgac actagttgtg tcagacccct
     2521 tttttctttt atagtctcaa tggaagatta gacgatttct tctagctctt ctgttttgaa
     2581 atgaagttgt ccatcaagta aagtaatcgt tacttttgat ttaggcataa catgtcctgc
     2641 aacgatttct ttagctagtg gtgtttctac ttccttagtg ataaatcgtt tcaatggtct
     2701 tgcaccatat gctggttcat aagcattttc agcaatccac gttttggctt catcactgat
     2761 tgtcagcagg atttcttgat gttccagacg ttgtgccaat tgcgccacca ttttgtcaac
     2821 gattcctttc acgttgtcta ggctcaatgg tgtaaataaa atcgtgtcgt cgattcggtt
     2881 caagaattct ggtttgaagt ttccacgtaa tagcgtgttg acttgttcag ccactgcttc
     2941 tggaatcgtt ccatcagcag tcacgccttc cagcagtaac tgtgaaccga tattgctggt
     3001 catgatcaga accgtatttt tgaaatctac tactcgccct tttgaatcag tcaagcgtcc
     3061 gtcatctagg acttgtaaca agatattgaa gacatcggga tgcgcttttt cgatttcatc
     3121 caacaagacg attgtataag gatttcgtct gacagcttcc gttaattgac caccttcttc
     3181 gtaacctaca tagcctggag gagctccgac tagacgagac acagcatgtt tttccatata
     3241 ttcactcata tcgatccgta ccatatgatc ttctgagtca aacaagttct cagctaacgc
     3301 tttcgctaat tcggtttttc cgacaccagt tggtcctagg aaaaggaaag aacctagagg
     3361 acgatttggg tcttgcaatc cagcccgtga acgaattacc gcgtcactga ccgcatcaac
     3421 tgcttcatct tgaccaatca ctcgtttatg aagtgtttca ttcaatttaa ttagtttttc
     3481 tcgttcacct tctacaagtt tcgttacggg gatacccgtt aatctgccaa cgacttgagc
     3541 aatctcattt tcggtaacgg actcttgaac catcttaatc tcgctgtctt ttgctttagc
     3601 ttctaattcc ttcaattctt tttctaattg aggtatcgta ccatgacgca atacagcagc
     3661 gcgttctaaa tcatagttat tttctgcatc ttctaattcg tgttttgctt tatcgatttc
     3721 agcacgtttt gctgaaacag aattgacttc ttctttttct gtttcccact gcatcttcat
     3781 cgcatttgct tcttcacgaa gttcagctaa ttcttcttgc aaatttttca agcgtttttt
     3841 acttgcatca tctgattctt ttttcagtgc agcttcttcg atttccaact gcatcaaacg
     3901 tctagtaact tgatctaatt ccgttggcat agagttcatt tctacccgaa tcgttgcact
     3961 ggcttcatcg atcaaatcga ttgctttgtc cggtaagaac cgatcagtaa tataacggtc
     4021 agataaagta gctgctgcta ctaatgcgtt gtcatggatg ttaacaccat ggtggatctc
     4081 aaaacgttct tttaatccac gcagaatact gatcgtatct tccactgttg gttctttgac
     4141 taataccttt tgaaagcgac gttccaacgc tttgtctttt tccatatatt gacggtactc
     4201 atctaaagtt gtggcaccga tcaaatgtaa ttcaccgcga gcaagcattg gtttcaacag
     4261 atttccggca tccatacttc cttctgtttt tcctgcccct acgatattgt ggatttcatc
     4321 gatgaacaag aggatacgtc catcactttt tttgacttct ttcaagactg cttttagtct
     4381 ttcttcaaat tcaccacgga attttgctcc agcgatcaat gcacccatat ctaatgaaaa
     4441 aatcgtttta tcttttaaat tttcaggcac atctttgcgt acgatacgtt gagcaagccc
     4501 ttcaacgatt gccgttttcc ctacaccggg ttctcctatc agaacaggat tattttttgt
     4561 ttttcgtgaa agaatacgga tgacatcacg gatttcttca tcacgaccga tgattggatc
     4621 catttttccg cttttgactt gctgtactaa atcaacgccg tatttttcta atgctttgta
     4681 ttgttcttct tggttttgtg aggtcactct ttctcctcct ctcatctctt cgatgttttt
     4741 acgcaattct ttttcagata atccgttttt attcaaataa acagttaacg gataattttt
     4801 taatttcatc aacgctaaaa taacgacttc cgttgaaaga aactcgtcgc cgaagctttc
     4861 acgtatttgg tctgcttcat tcaataatcg aaagaaattc tggctgaggt tttgtccata
     4921 ttggacgttt ccaccagaaa caactggata ctcatccagt agacgatcaa tttcatgttc
     4981 aaaagattcg acatccaatc cggcatccgt atagaaattt cgaccaaaat gattagattg
     5041 taaaaagatt ttccatacat gggcaatgtc gatatcttga tgttttcgag tgaccgcaat
     5101 ctgttgagct tcagcaatcg cttcttgaag cgtcgtagtc attttctca
//
LOCUS       DACDHJ010000156         5149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDHJ010000156 DACDHJ010000000
VERSION     DACDHJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.159"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..1078
                     /locus_tag="IV223_002106"
     CDS             86..1078
                     /locus_tag="IV223_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8712365.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1094..1978
                     /locus_tag="IV223_002107"
     CDS             1094..1978
                     /locus_tag="IV223_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8712366.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2027..3634
                     /locus_tag="IV223_002108"
     CDS             2027..3634
                     /locus_tag="IV223_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8712367.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3635..5119
                     /locus_tag="IV223_002109"
     CDS             3635..5119
                     /locus_tag="IV223_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8712368.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
BASE COUNT         1616 a          834 c         1137 g         1562 t
ORIGIN      
        1 tataaacgaa aatagtggta acgtttccaa aatgcatctt cgttaaattt ttattttttt
       61 aaacgaaaat ggaaacgttt ctaaaatgta ttgggaggag attgaattgg aaaagacatt
      121 gggaccaggt ggtgagataa gggcggtaca acgggataaa aaaaatattt cgaagtggga
      181 aaaattaaag aaacagttgc ggcgtgataa gtggatgtac ttgttgctct taccaggtat
      241 catcatgacg tttatcttta agtatgtgcc aatgtacggt gctgtgattg catttaaaga
      301 ctataatcct atgctaggaa ttttggaaag tccttgggta ggactagatc actttaggga
      361 atttatcagt tcaccaaatt ttacgctttt gctgagcaac acattaaaaa ttagtattta
      421 tggtttattg ttaggttttt ttccaccaat tattttagca ctgagcttga atcaaattat
      481 caataaaaaa ataaaacaga aaatccagtt gattctatat gcgccgaatt ttatctcagt
      541 agttattatt gtggggatgc tgtttttgtt cctatccgca aatggaccta ttaatgggtt
      601 agtagaaaag gttacggggc aaccaattat gttcatgtct aatccagctt atttcagaac
      661 gatttatatt ttatcgggaa tttggcaagg aatcggctgg tcatccattc tttacaccgc
      721 aacgctggcg ggagtcagta ccgatttata tgaggcagca aatatcgatg gggcaaatat
      781 tttacaaaag atttggcata tcgatttgcc tgcattgagg cctgtgatgg tgatcagctt
      841 catcttggcc gccggcggta ttatgaatgt aggatatgaa aaagcctact tgatgcaaac
      901 ttcaatgaac atccctgcct cagaaatcat ttccacttat gtatacaaag tcggcttgca
      961 atcaggtgat tatgcctact ctgctgctgt tggtctgttc aatatgatta ttaatgtcgt
     1021 tttattactg attgtcaaca atataaccaa gcggctaaac gatggtcaag gcttgtaatg
     1081 aaaggagaaa attatgaata gtgctttatc aaaaatcgac cgtcaaatct tatggttgaa
     1141 tcgagtgatt gtaacttttt tagtcgtcat caccattgtt ccgctgattt atgtactggt
     1201 agcttctttt atggacccag ctactttgat caataaaggt ataagcttca atccgaaaga
     1261 ttggacaata cagggatatc aacgggtatt tgcagatgat tccattttac gcggttttgt
     1321 aaattcgttg ttttactctt ttagcttcag cttattgact gtatttatta cgatgatcac
     1381 tgcgtatcct ttgtctcgac ctaacttggc ggggaaaaag attattatga ctttttttgt
     1441 cattacaatg ttcgtgggtg gtggcttgat acctacctat ttattggtaa agaacttggg
     1501 catgttagat agtgtatggg cgattatcgt accaggtgct ataaatgtgt ggaatattat
     1561 cttagcccgt acctatttta gtgctttgcc aaaagaactt tctgaagctg ctactatcga
     1621 tggcgccaat gacttgcaaa tactgttcaa agtcatgctt ccattagcga aaccaattat
     1681 gttcgtattg tttttatatg cctttgttgg gcaatggaac tcatactttg atgccatgat
     1741 ttacatcaaa gatcccgact tacagccgtt gcaactcgta ttaagaaata ttttgatcca
     1801 aaaccaacct tcacaagaca tggtgggagc caatactgcc atggcagaaa tgaaacaaat
     1861 tgctgagttg atcaaatacg caacgatcgt catttctagt ctgccattaa tcataatgta
     1921 tcctttcttc caaaaatatt ttgataaagg tgttttagtt ggttctgtta aagggtaagt
     1981 attgttcact atttgtatga ataaaaatta taaaggagat tttaagatga aagcaaaatg
     2041 gatgctgcca atcgttttta cttccgtact aattttgggg gcttgtggag gctcaaaaga
     2101 aaagagtaaa tcaagccctg attatgaact agaaaatgtc tcttttccat tgaaagaaaa
     2161 agtatcttta aaaatgatgt ctcaaagtgc ccctttggct cccaatgatc ctaatgacaa
     2221 attgattttc caacgattag aagaagagac caatctccat attgattgga caaactacag
     2281 tagtgatttt gccgaaaaaa gaaatttgga tatcgctagt ggtgatttac cagatgccat
     2341 cttcaacgcc gctgcaggtg actatgattt attaaactgg gcggaaagtg gcgttattgt
     2401 accgattgaa gatttgatcg aagattatat gccaaatctg aagaaaattt ttgatgaaaa
     2461 ccccgaatac cgacaaatgt ctactgcacc tgatggtcac atttactcta tgccttggat
     2521 tgaagaatta ggcgaaggaa aagagtcgat tcatacagta aatgggatgg cttggatcaa
     2581 caaggagtgg ttggataatt tgggtcttga aatgccgcaa accactgatc agttgatgac
     2641 tgtgttggag gctttcaaga cgcaagatcc aaatggtaat ggtgaagcag atgaaattcc
     2701 ttttagcttt attaacaatg gaggaaacga agacttaaaa tttttaatgg gggcattcgg
     2761 tctaggtgat aatgatgacc acttagtcgt tgataacgat ggtcaggtac aatttactgc
     2821 taacaaagaa gggtataaag aagccatcaa atatttcaat gaaatgaata ataaaggtct
     2881 tattgataaa gaggctttcg aacaagattg gaatacgtat gttgccaaag ggaaagaaca
     2941 taagtatggc gtgtacttta cttgggataa agcaaatatc acgggtgcag atgatagtta
     3001 tgacgtcttg ccagtcttag cgggaccaga tggaacgaaa catgttactc gtacgaatgg
     3061 catgggtttc tcaagagatc gtttcgtaat tactagtgcg aataaaaact tggaacttac
     3121 agctaggtgg atagataaaa tgtatgaacc gttacagtct gtccaaaata actggggaac
     3181 ctacggtgat gaagcacagg cgaatatttt tgaattaaag gatggtatgt taaaacattt
     3241 gcctttagag gggacagcac cgggggaact aagacaaaaa accgaagctg gaggaccatt
     3301 agcagttctt gattcttatt acggcacagt cactactatg ccagatgatg cgaagtggcg
     3361 tttagatttg atgcatgaca cgtatttgcc atatattaca gaagacaata tctatccacg
     3421 tgtcttttta gaaaaagaag aaacagatcg attagcaaaa atcgaaacgg atatgtggga
     3481 ttacatctat cgcaaaagag cagagtggat caccggcggt aaaatcgatg aagaatggga
     3541 agaatacctt gctgaattgg aacgtgtagg tttggaagaa tggattcaaa taaaacaaac
     3601 tggctatgat aattttttga agggagcaca ataagtgtta gtagaagaaa cggcgatttt
     3661 ggataaagca aatcgattta ttgaaatgat gcgaggaaaa gttaaccctc agtatcgaca
     3721 aaactatcat ttttccgcac ccatcgggtg gataaatgac cccaatgggt ttatatatta
     3781 caaagggcat taccatctct tctatcaata ctatccttac gatagtcgat gggggcccat
     3841 gcattggggg catgcgcgca gtagtgattt ggtccattgg gaagatctac cagtagcgtt
     3901 agccccagat caaccttatg acagcggcgg gtgtttctca ggaagtgcaa ttgaaagaga
     3961 cggaaaacta tatttatttt atacaggctg tcaagaaatt gatggtatta gccatcagca
     4021 tcaatgtttg gcggtctctg aagatggtat tcattttagc aaatacagtg gtaatcctgt
     4081 ggtagcagaa aaacagctag gtgaaaatgg gctaatacaa gaatttcgtg atcctaaagt
     4141 aattgttcac aacgatggtt actatatggt agtagctact aaatctacac agtctactgg
     4201 tagaatagtg atgtttcaat cgtcagattt gtatacttgg cacttttact ctgtgctgct
     4261 agaagggact tcagagcaag gaataatgtg ggaatgtcct gatttattcc atttagacgg
     4321 acgagatgtc ttaatcatgt ctccgataca aatcaagaag aacggactgg aatatcataa
     4381 tatcagttct accatggctt gtattggtga ggtaaactgg gaaacaggtc agatgatagt
     4441 tgataatttc catgaaatgg attttggaat ggatttttat gcaccacaaa caacaagaga
     4501 tcctaatggt ggacgggtga tgattgcttg gatgcagatg tgggatcgta cattgcctac
     4561 acatgagtta ggccatcttt gggcaggctc tatgagtttg ccccgagagt taagagtgaa
     4621 aaaaaatcga ttggtacaaa aacccatctc aactgtatat agtcatttag tttatcaaca
     4681 aggcagtgaa aatattgcag tgactgacac atcagtcgta tttcgaaacg cgctgtctga
     4741 ttcttcatat ttacatctag tcgcagattt ggagaaagct gaagttttta caattgaatt
     4801 actgcggtct aagacggatt gtctcaaact agcctatgac actgctacac aggtttttga
     4861 attggatcgt cagaaactag gaagagagct tacaggggcg gaagaagacc ctctgaatgt
     4921 acgaaaagtt gaagtacctt tgattgataa tcagctcgtt cttgagattt ttagagatac
     4981 ctcatcaatt gaagtcttta ttaatgggtt agaagttatg acggctactt tctatgagct
     5041 ggaaaaagct cgagatatct tgtttcattc gatcggagaa agtcgtattc aagcttttga
     5101 aacagcgagt gtgaaataaa tgtttgattg cttaggcgat gaatcaatt
//
LOCUS       DACDHJ010000157         5143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDHJ010000157 DACDHJ010000000
VERSION     DACDHJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.160"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..295
                     /locus_tag="IV223_002110"
     CDS             <1..295
                     /locus_tag="IV223_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712369.1"
                     /translation="DPKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILI
                     GTNSTVYQEAEERVEGKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            314..1906
                     /locus_tag="IV223_002111"
     CDS             314..1906
                     /locus_tag="IV223_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712370.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1918..2253
                     /locus_tag="IV223_002112"
     CDS             1918..2253
                     /locus_tag="IV223_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712371.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2400..2594
                     /locus_tag="IV223_002113"
     CDS             2400..2594
                     /locus_tag="IV223_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712372.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2626..4998
                     /locus_tag="IV223_002114"
     CDS             2626..4998
                     /locus_tag="IV223_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712373.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2065 a          814 c         1068 g         1196 t
ORIGIN      
        1 tgatccaaaa atcgaagaaa aaataaatcc aattcaaatt aaattgaatg caatgggaaa
       61 agaaatttct attttaaccg aaatggtttc agagtttgcg gatcttcatt tgaaagatac
      121 aggaatacta attggtacaa attcaactgt ttatcaagaa gcagaagaac gtgtggaagg
      181 aaaaattaaa tccaatcaaa ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga
      241 atctgttcat accagagtag atccatttga tccaacgaag attctaaaaa gataaaagta
      301 gaaggagaac gtgatgaata aatcgcctgc agttattctt acttgtcaat ttaccttgcc
      361 caataaaaaa tctttttcaa catacattga ttatatgaca agagaaaaag cactggaaga
      421 aatggaacga cgtacgcccg aagaagaaca ggaactgcaa cgaatcaaga atgctttaga
      481 ggatttttat attcctgaag gagaaacttt ttcggaaagt aagacggata aagaattcag
      541 tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga atcaattttg acaagttaga
      601 ggaacaagat tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa
      661 gaaagaactg acgcaaaggg aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa
      721 atatcaaaca gagccacata aaaaagcttc aggaaatctt cctggagttt tttctatggc
      781 ttcagacgaa gtgaaaggaa gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca
      841 acaaaaaggc tcaatcattt atcaagatgt tatctctcac gacaattcgt atcttgaaaa
      901 aatgggcttg tacgatccca aaacagatac cttagatgaa gaaggtttga aagcagctgg
      961 aagaaaaatg atggagacat tattcgagaa agaacaattg aatgaaacag gttactggat
     1021 ggcaacgatt caccgtaaca ctaagcatat ccatattcat tttgcagttg ttgaaaaaga
     1081 aaatacgcgt aaaccgtata ctgagattga aaatggtgtg agttatatcg taccgaaggg
     1141 gaagagaaga caagcaacat tagacgcaat gaaaaccaat tatgtacatg agttagaacg
     1201 tttctcttat gaaaaggatc gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa
     1261 aagtgatttg cgtaacacct taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca
     1321 cgccatgaac ttattaaatg aagtatataa aaccctacct gaaaaacgtt cggaatggaa
     1381 ttatggagat gaaaatcgaa ccaagctttc accatttaca aggggaaaac ttgatgagtt
     1441 aaccaaatat gttttaacaa aagaacctgg atatcaagaa tatatacaga tttcaaaagc
     1501 attgaaagaa gaagccaaaa gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga
     1561 aaaaaacgcc ctctttgatt taaaaaagcg cacaggaaat gcgattttaa cagaactgaa
     1621 gagacaggat agtcaaataa aaccattaat agaaagtgtc aatcagatgc aatctattta
     1681 caatgaacga tcctttcgaa cactcaaaca gttgccaaca aatgaataca agtatagtta
     1741 tcaagaattc gtaaagcgta ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt
     1801 gcgtcaatta aatcgaacaa ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata
     1861 tgaagagcaa cagcgaagaa tccaaatgga acaagaaaga caataggagg aagagaaatg
     1921 aaacaatatt tagatcaatg gaaagtcata gaaggatcac taaaagaaga acgaatcgaa
     1981 caacttcctg attgtttaga aaaagagcat ttgtttcaga taagcgaaat gttgagaaac
     2041 gaacagtttg atccaaatca atttttagtt gttgagtacc cagctactgg tgtgtattgt
     2101 tgtaatcatg tgaagggtga aaaatatttt attattcaag aatacgaagg taagttagcg
     2161 ccttattata ctacttggga aataaatgaa gagggaatca ataatttccc atgtgaaagc
     2221 attgaagagt ctatttctct cacggaatgt taaataaata gagacgtaaa agagtcctga
     2281 acgtgattgt ccaggactct ttttcaatga attgttttcc gccaattcac tgagtggtgc
     2341 gattcaattt tattatatca aaagaaagtg agattccaaa gtgaagggag gaagaagaaa
     2401 tgactcagac aacgattggt gttgtggttg aaaaaagcag ggataatctc gtttttgtga
     2461 cagaaattca aacaggtaga gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa
     2521 atggcaatat tttaacgtta aatctgacta cgaagatgtt cgttgatgca gtagaaaatt
     2581 atccatttgt ttagacaaac gatattttac aaggaggaaa cggacatggc agaagaaaaa
     2641 gaaccactag ataatagtcg tttgggaaag tccaaaagaa aattagtacg gttgcaaaac
     2701 gaattaaata atcaatttca aaagctattt gatcaccaaa aattgacaaa tgggcaacca
     2761 atgaatgaca aacgaaatgg tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa
     2821 atacaaagtt tgagagaaga gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga
     2881 caagaagagc taaaggaaat gggttataac aagtacggtg gcctggacat gacgatcgaa
     2941 aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa aaggtgaatc gactttttca
     3001 gctgccacga tccgaaaata ccaacgaaaa ttggaaacat tagaacagct gaaagaaaaa
     3061 tcagagaaag gaaaagaaaa tcttcttcct gaagtacaag cgatcattga cagcggacga
     3121 gtgacgcaat ggaaaaagaa tccaactatc tattttctga aaggctatcg aaaagtcgct
     3181 ttagaactca atgaaaacgg gggatttgag gaatctaaaa aataccaagc cacgtcggaa
     3241 gaagaaaagg cagctgttcg tgaattactt gaagaagcta cacctactga ttctgtgaag
     3301 gagaaagaaa ccgatcattt ctatcgagta gaattcaacg aaaatgatcc agcaaaaggg
     3361 ttgaaaagtt atggtgggga gatcgtaaca ctagaattga tcgatcaact caaaacactg
     3421 gataaccaat acgccgcaga agcaggctat tataaattct attttgaaga agtgaagaat
     3481 ggagaagtag tcgatcagat acgaatggat ctaggggatg gcgtcaatag taatcacccc
     3541 atctatttag agttagctag attgtgcgaa caatcacctg aaaaggaaca agaacaaaca
     3601 gtcaaaaatg aacctatttc aaaagtacaa aacgagatta aagagacaaa aacaaaacaa
     3661 aagggttatc aaaaacgaac ggcagaagag attaagcaag aagtcaaatc actttcccaa
     3721 caggcgcttg atgcggtgaa aaagcatact cattcacctg aagacgtcaa agaacttttg
     3781 aattttatgt ctcgttttcc agaacgatcc tttagaaatc aattattgat cgaggaacaa
     3841 tttccaggcg caacggcttg tttaggtcgt gccgcgttga aaaaagaagg aatctatatc
     3901 aaaaaaggag aacaagggaa caagatcttt gtcagaaaaa cggtcaaagg attttatgag
     3961 aaaggacgtg gctttgttcg agaaacagag gctacaccag aagataaaaa acgaatcgaa
     4021 agtggtcaaa tcgaagtgat caaaaaacct tactacacga ttgaaaaagt gtttgatatc
     4081 acacaaacac aattgaaacc tgaagactat ccgaaaatct tcccgaaccg cgtctttgat
     4141 tttcaattgg atcaaacagg gcagacagaa cttcaagttg ggattcaagc ggtagccaaa
     4201 aatattggaa ttcaaattcg agatatgaca gaaagtgaag tctatcaaag agaattagga
     4261 caagcacgtg gggcgtacgt tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat
     4321 actcgaaata ctcctacgca gcatttagcc acaagtattc acgaattagc ccatgctaga
     4381 atgcacaaat tttcagaatt agatactgca acaaaagaac tacaggcaga aatgacctcc
     4441 tatattgtgt gcaagcattt tgggatggac acctcggaga aagctattcc ttatattgcg
     4501 acatggacga agaatagtca gacgttggat gataaggaag ctgcggaacg tgggaaaatc
     4561 atgaatgatg tcagtcgtgt cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat
     4621 caatacagag aaagagaacc agagatccag ccagcagaag tgaagcagga gcataaaaat
     4681 actgtagaaa cggcagaaca aaagaatatt gtgcccgttg gctcactttg gatcgacaag
     4741 aaagacggga aagtcctgga agaagacacg ggccgaacgt tacatttgaa ggatgcggcg
     4801 tttattgaag atccgcagca taatcaagtg attttaaaag gaaaaagtta tggaaaagag
     4861 atccaaacgg cgttaccttc agaagaattt gcgctaggaa atccattccg attttcactt
     4921 gagacagtgt cagaagtggc aaaacgaaaa aatgaaatct attatgaaca acaattgtca
     4981 caaaaagaac gcgtataaaa caagcaagaa agactatctg aaaaatggat agtctttttt
     5041 cttttgggag gtttgaaaat gacagagaaa aaaatgagtc tcattgatcg atgcaagcaa
     5101 atggatattg tggattttgc acgaaacaat ggtttagcgg tgg
//
LOCUS       DACDHJ010000158         5107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDHJ010000158 DACDHJ010000000
VERSION     DACDHJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.161"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            446..2038
                     /locus_tag="IV223_002115"
     CDS             446..2038
                     /locus_tag="IV223_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8712374.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2051..2791
                     /locus_tag="IV223_002116"
     CDS             2051..2791
                     /locus_tag="IV223_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8712375.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            2959..4308
                     /locus_tag="IV223_002117"
     CDS             2959..4308
                     /locus_tag="IV223_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8712376.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(4436..5026)
                     /locus_tag="IV223_002118"
     CDS             complement(4436..5026)
                     /locus_tag="IV223_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8712377.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         1562 a          963 c         1059 g         1523 t
ORIGIN      
        1 tgttttttcg gtaaaagtaa agatttctat atttatcagt attttttcaa aaaccagcag
       61 ttatagattg acaaagcctc tcgagtatcg taaacttttt ttgtataaat aactgaatac
      121 gagatgcctc aatcaatgag gtagaggtcg cgagaatcat taaccctgtg gagtgaagca
      181 aacatggaga agcaggaata gggaggatcg ccgaaatgtg cacttttttg cttggagtgt
      241 atgttgggac gcaagttaag agcttgcgga ctgtcttagc agtgatgcta agttgcgcta
      301 tgttttgtga gaggtttatc accttatgga attttagcgg cctttttata tctataaatc
      361 ggtcgctttt tattttttca gataaagctt taccgcattt catctagacc gctcgtgtat
      421 catataatca gaaagggaga aaaaaatgaa aaaaaatatg gtttcagttg ccgtactatt
      481 tgcttttgtc ttctcactat tcagtgtggg atctctggca aatgcagatg aaaaaacacc
      541 gagcgatgag tttcgcgtag gtatggaggc tgggtatgca cccttcaact ggtctcaaca
      601 aacagatgag aataatgcct tgccaattca aggccaaaat agttacgccg gcggttatga
      661 tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa aaattagtca tcgtccaaac
      721 gaaatgggat ggtctggctc ctgcccttca atcaggcaag atcgacgcga tcatcgccgg
      781 aatgagccct actgctgaac gacgaaaaga aattgatttt accgatcctt attacgaatc
      841 acaattagta gtagtagttc aaaaagatag caaatatgca aaagcaaaaa gtttagcaga
      901 tctttctgga gcaaagatca ctgctcagtt aaatacattc cactatactg tgatcgatca
      961 gataccagat gtgcagaaac aacaagcaat ggataatttc tcagcaatga gaacagcttt
     1021 agcttctgga atgatcgatg gttatgtcag cgaacgtccg gaaggtgtaa cggcaactag
     1081 tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca gatggcttcc agacaaaccc
     1141 ggaagatgtt cagatttctg ttggtatgcg taaaggcgac ccggatatcc aaaaagtaaa
     1201 ccagattctt tctggcatct ctaacgatga acgaaccaaa attatggacc aagcagtcaa
     1261 agatcagcct gctgcaactg atagtgaaga aaaacctggc atcatttctg atttcactac
     1321 gatttggaaa caatacggca atatgttctt acgaggtgct ggacttacct tgttcatcgc
     1381 attgatcggg acagttgtcg gtactacact tggattactg attggtgtct tccgttcggt
     1441 acctgaatca ggaaatgcac tgactcgctt cttccaaaaa atcggaaatg cacttctttc
     1501 tatctatgtc gaagtattcc gcggaacacc gatgatggta caagcaatgg tcatcttcta
     1561 cggtctggca ctcgcttttg gcatttcatt gaaccgaacg attgccgctt tgtttattgt
     1621 ctctctcaat actggagctt acatgacaga gatcgttcgt ggggggattt ttgcagtaga
     1681 tgaagggcag ttcgaagctg cgcaagccat tggtatgacc catggccaaa cgatgagaaa
     1741 agttgttatt ccacaagttt tacggaatat cttgccagca acaggaaatg agtttgttat
     1801 caatatcaaa gatacagcag ttttaagtgt aatcggcgta gctgatttgt tcttccaagg
     1861 aaactctgct tccggtgcga atttccaatt cttccaaaca tttacgatcg tcgggatcat
     1921 ctacttagtc atgaccttta cgatcaccag aatcctgcgg gcggtagaaa agaaaatcga
     1981 tggtccttca gcttatacca agcttgaaga aatcgacaat tcaaatttgc aggaataagg
     2041 aggaagaatc atgaatgaaa aaattattga aatcaaacat ctaagaaaaa gcttcggtga
     2101 aaatgaagta ttgaaagata tcagcgtaac agtcaataaa ggtgaagttg tgacgatcat
     2161 cggttcttcc ggttctggta aatctacttt cttgcgctgt atcaatttac tggaaaaacc
     2221 aacaggcggc gagatcattt ataatggaga aaatgtatta gctcctaaat ataatttacc
     2281 taaatatcga acgaatctag gtatggtttt ccaatccttc aacctgttca acaatatgaa
     2341 tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg aaacgagata aagaaactgc
     2401 caaaaaagta gcaatcgaaa atctggaaaa agttggtatg gatcgttata tcgaagccaa
     2461 accagcacaa ttatctggtg gacagaaaca gcgtgtcgca attgctcggg cattatcaat
     2521 gaatccagat gtattgctat tcgacgaacc tacttcggcg ttagaccctg aaatggttgg
     2581 cgaagtgctt aatacgatca aagacttagc acatactggc ttgacaatgc ttatcgtgac
     2641 gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc atttttatgg ataaaggtgt
     2701 gattgcagaa gaaggtacac cagaagatat ctttgtccat ccgaaagaag aacgtacaaa
     2761 agaattcctt tcacgtattt taaatgctta ggatcaatag ctgattggga caaagagctg
     2821 cggtgtttac cgcagttctt ttttatgaat tactcataaa aggtaaattt ctgaaaattt
     2881 tggaatctta gttgtgtttt tcagaaaact ctcttaatat ggaagtaatt aaactttttt
     2941 agttaggatg atttaaaaat gaatgaagag caagaactgc agcgtggact gaaaaataga
     3001 catgtccaac tgatttctat tggcggagcg attggaacgg ggttgttttt aggttccgga
     3061 aagtctatcc agctagctgg cccttcgata ttgctcgcct atcttattac tggatgcatc
     3121 tgttttttca ttatgcgtgc gttaggcgaa cttctgttat caaacaccaa taaccattct
     3181 tttctggatt ttgttgcaga atatcttggc aaaaaagcgg ctttcatcac tggctggacg
     3241 tattggttct gctgggtatc gattgcaatg gccgatttaa cagccatcgg aatgtatgta
     3301 cgctactggg cgcctggtgt tcctcagtgg cttcctgaac tgattgcatt aggtcttctt
     3361 ctttgcttga atatggtggc tgtctcattg tttggtgagt tagagttttg gtttgcattg
     3421 atcaaagttg tagcgatcat tgcctttatc ctcgtaggag cttatatgat cctcacccac
     3481 tatacgacag atattggcac agccagtatc acgaatctat ggtctcatgg cggattcttt
     3541 ccaactggtg caaaaggatt tatcttagct ttccaaatgg taacctttgc ttttgctggg
     3601 atagaattag ttggattagt cgctggagaa acagaaaatc ctgaaaaagt gttgccggaa
     3661 gctatcaata atattcctat caggatcatc ttattttact taggatctct ttttgtcatt
     3721 atggcaatct atccatggaa cagcttgaat ccagataaca gtccatttgt agaagtattt
     3781 tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt tagtcgtatt aagtgcagca
     3841 gcttctgcct gcaacagtgc aatatatagt acaggacgta tgcttcgttc tttagcgcaa
     3901 gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac atcatgtgcc agggaatgct
     3961 ttgacatttt ctactatcgt cattttcatt tctgttatct tgaattacgt gatgcctagt
     4021 gaagtattca cactagtaag cagtattgcc actacttgtt tcttgtttat ctggagtatg
     4081 cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg gcaaaaaagc tcattcattt
     4141 aaaatgccgt tgtacccatt tagtaattat ctggtatttc tttacatggc ctttgtgtgt
     4201 gtcgtcttgt tcctaggaaa agatacgaga atcgctttat tactaacacc tgtgtggttc
     4261 ttgcttctat tactaatcta tcatatgaaa tatgaacgta agaagtaaga aagtagttgt
     4321 gacagaagtg gtcagcttcg agaaataaga aggaatttac gaaaattgct tcttaaattt
     4381 ttgtgaaatt tcggcttatt tcttggagat aaaagcttct gtcccagcct cttttctatt
     4441 tccccggaaa caaccgctga taataggcat ctgctttttt ataggaaaaa atccaaatac
     4501 cagatacaaa aaaacgtccg aataacccga tcaacggtac aaaaaagaca ccggcaatca
     4561 agcataagtt aatcatcatg gagataagac ttcgacggtc ctttgacaaa actctcacga
     4621 catgggaatc tggtttattt gctttcatga tttgcctcat caagatacca aaagaaaaac
     4681 tccacatcgt gtatactaag acataagcca tctcaggcca aaaagcttca gggttacgcc
     4741 cgacccaact agtaacagaa ggacataaag tcagccaaaa gagcaaaaag atattcgacc
     4801 agaccactcg gtaattaatt cgttctgcca tctgaaatat acgatgatgg ttcacccaga
     4861 tcaccgcaac aaatataaag ctgatggtat aagctaataa tgtatttcgg atgacccata
     4921 aagaatgaaa gctagatgta ttagggatct tgatctctaa tacaagaact ctcaaaacaa
     4981 tggctaccac tccatcagta aatgcttcta atctcgactt tgtcacgatg attcctcctc
     5041 agatgattca gcgcttttat tttatcactt acgttattaa ataaccatcg ttttatatag
     5101 ggaattt
//
LOCUS       DACDHJ010000159         5068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDHJ010000159 DACDHJ010000000
VERSION     DACDHJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.162"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            515..2026
                     /gene="gadC"
                     /locus_tag="IV223_002119"
     CDS             515..2026
                     /gene="gadC"
                     /locus_tag="IV223_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8712378.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            2039..3439
                     /locus_tag="IV223_002120"
     CDS             2039..3439
                     /locus_tag="IV223_002120"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8712379.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3604..5040)
                     /locus_tag="IV223_002121"
     CDS             complement(3604..5040)
                     /locus_tag="IV223_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8712380.1"
                     /translation="MLETYLEKSIFRQVYICEQLHEKGTIQIREIADQLNVCPLTVTN
                     DLECITDILHQQIISVEKSRSSFTVAFDSSFSRLALTQLIYQRSDFFNVLGHYLSGEH
                     NWSNIADTTFISLSKVYNLRNELINFFKQMQYLNADGHFEIPEKDYRSLLLTIIYETN
                     RTELIQLNKSIIQSGQQLIDYVEKHFFSRSYPTVEKQFILLGIAIGLQRSKTNPIYFS
                     QEEKRVARHTPLFQLIQKGLEQLSFSLCHDEDECLYIYFLFNSRNYLCDNFELLQKDL
                     NVVYQNHVADNPLVIELSQKLILSLNISEENQILFDKALLPFIRSLWADIQIFQPDKT
                     YLLNEEQRALYLEVHEILEQWRKQNNLFLRWNENSIRKLTISLSLLNEHKRKSPIEVF
                     IVAPSDFRYLYYRQQLEDILGEHFSISNIICKQLREIVDDTFFCTQRIILCDSSLYQE
                     GLGSEKTIIYPITFQTIHTVIDQLNKQI"
BASE COUNT         1584 a          830 c          957 g         1697 t
ORIGIN      
        1 cattcaaatc gcctttgttc gctagagtct ttaggatttc tgtagtaaaa tcgttcatat
       61 ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag ggacttcctt
      121 ttttctattt acacaaaata ttttacactc tcaacaaaag tatcttcgat taatacttgc
      181 tcaagttatg tgcagctacg aaaaagtagg taccgaggta ctttcttttt cgcagctttt
      241 tttggactta tgagaagtac attcgctgga agaatacgat ataaaacgag ctttttcact
      301 aaagatatta atagagtgaa agaaacaagc ttttgttgtc agttttttca cttttctaaa
      361 aatgaaaata aaaaattctg gaagtggaga agaattttgc ttgctatact ttgtcttgta
      421 gaaaagaggt agacaaaata atgcaagctc aaataagcga ttttccataa ccttggtagg
      481 aatgacaaat taaataggaa agggaagtaa taatatgaat aaaaaaaagt tatctctatt
      541 cggttttttt gccctgacag cttccatggt tttgactgtt tatgagtatc cgacctttgc
      601 aacatcacaa ttacatttgg ttttctttct tcttttggga ggtattttat ggtttctacc
      661 agttgcttta tgtgctgcag aaatggcaac agttgaagga tggaaagatg gtgggatttt
      721 cagttgggtt agtcaaactt taggagaacg ttttggtttt gcagctattt ttttccaatg
      781 gtttcaaatt acagtaggtt ttattacaat gatctacttc atactaggag cgctgtcgta
      841 tgtaattaat tttccagagc ttaatacaga tccatttatc aagtttattg gtgtattagt
      901 cattttctgg gtactgactt tttcacagtt gggcgggacc aatcgtaccg ctaaaattgc
      961 taaagcagga tttgtcattg gaattgttat tccttcgatc attctctttg gtttagcagc
     1021 agcctacttt atcggcggta atccgattca aatggctatt aatgcgaaag cttttattcc
     1081 tgacttttct cagctttcaa ctttagtagt atttgtatct tttatattgg cttatatggg
     1141 cgtagaagct tcagcatcac atattaatga gctggaaaat ccccaaagaa actacccatt
     1201 ggcaatgttt ttactagtga ttttggccat cgccttagat gcaatcggcg gtttctcagt
     1261 agcagcggtt attccacaac atgacttgtc tctcagtgct ggtgtaatcc aaacatttga
     1321 aacgcttatt ttacatttta acagtcattt acattggttg gtaaaagtga ttgctctcat
     1381 gattgcctgt ggcgttatgg gtgaagttag ttcttgggta gttggtccat cacgagggat
     1441 gtttgcagct gctcagcgag gtctgttacc agttttcttc cggaaaacta ataaacaaga
     1501 cgttccagtt ccgttgatta tgatacaagg aattatcgtt actatttggg cagcagtctt
     1561 aacttttggt ggaggaggca acaatttatc attcttagta gcaatctcct taactgttgt
     1621 catttatctc gtaggatatt tactattttt cctaggttac tttgtattag tttttaagaa
     1681 aaaagactta aaacgaactt acaatgtacc aggaaaaact gttggcaaaa cgattatcgg
     1741 tggaattggt tttattttat cagtgttcgc attgcttatc tcttttgtac caccagcttc
     1801 aattgcagct agtgagcacc gaacatacca aatcatacta cttattagct ttgtagtgac
     1861 tgttctttta ccttttatca tttatgaagt acatgataaa agtgctcaca caactattga
     1921 ggaacctact cacgtgaaag ctgaagacgt taatcccgct gtttatccag ctgctagagg
     1981 agagcaccac gttacaaaaa atgatgaaca tattttaaaa cattaggagg aattcattat
     2041 gttatacgga aaagataatc aagaagaaaa aaactatttg gaaccaattt ttggctctgc
     2101 aagtgaggat gttgacttgc ctaaatataa gttaaacaaa gaatccattg aaccacgaat
     2161 tgcttatcaa ttagtacaag acgagatgtt ggatgaagga aatgcgcgat taaacttagc
     2221 tactttttgt caaacgtata tggaacctga agcagtgaaa ttgatgaccc aaacgttaga
     2281 aaaaaatgca attgataaat cagaataccc acgaacaacg gaaattgaaa accgctgtgt
     2341 aaatatgatt gctgatttat ggcatgctcc aaataatgaa aaattcatgg gaacttcaac
     2401 gatcggctct tcagaagcct gcatgctggg tggtatggcc atgaaatttg cttggcgtaa
     2461 acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa cctaacctgg tgatctcttc
     2521 tggttaccaa gtttgttggg aaaaattctg tgtatattgg gatgtggaac tgagagaagt
     2581 cccaatggat gaaaaacata tgtcaattaa tctagatact gtcatggatt atgtggatga
     2641 gtacacaatt ggtattgtag gtattatggg tattacttac actggtcgtt atgatgatat
     2701 caagggtctg aatgatttag ttgaagctca caataaacaa actgactata aagtatacat
     2761 tcatgttgac gctgcatcgg gtggctttta tgcaccattt actgaacctg atctagtttg
     2821 ggattttcaa ttgaaaaatg ttatctcaat taattcttca ggtcacaaat atggtttggt
     2881 atatccaggt gtgggttggg tcttatggcg tgaccaacaa tacttaccag aagaattagt
     2941 atttaaagtt agttacttag gtggagaaat gccaactatg gctatcaact tctctcatag
     3001 tgcagcacaa ctaattgggc aatactacaa ctttgtacgc tatggctttg atggttatcg
     3061 tgatattcac caaagaactc atgatgttgc tgtttattta gccaaagaga tcgaaaaaac
     3121 tggtattttt gaaatcatta atgatggatc agaattgcca gttgtgtgct ataagctgaa
     3181 agaagatccc aatcgcgaat ggacactata tgatttatct gatcgtctgt taatgaaggg
     3241 atggcaagtc ccagcctacc cattacctaa agacttggat caattaatta ttcaacgctt
     3301 agttgttcga gcagactttg gaatgaacat ggctggtgat tatgtacaag atatgaacca
     3361 agcaattgaa gagttgaata aagctcatat tgtttatcat aaaaaacagg atgtgaaaac
     3421 gtacggcttt actcactaaa attcttcaga gaaatataaa agaagttaat taactaataa
     3481 aaggagttgt gattaccaca cagctcctta aatctatttg aataaatata tgatattcat
     3541 ttgttacact ctcaatctta gctgttgttc tattcatcat tactcttatt gtaagtagtt
     3601 cagtcaaatt tgcttgttca attgatcgat aacagtatga attgtttgaa aagtaatcgg
     3661 atatataatt gttttctcac tacctaagcc ttcttggtat aaagaactat cgcaaagtat
     3721 aatccgctga gtacaaaaga aagtgtcgtc tactatttct ctaagttgtt tacaaatgat
     3781 attactaata gaaaagtgtt ctcccaaaat gtcttctaac tgttgtcgat agtataaata
     3841 cctaaagtct gacggagcta ctatgaacac ttcaattgga gatttccgtt tatgctcatt
     3901 taaaagactt aaactaatgg ttagtttacg aatagaattc tcattccaac gcagaaataa
     3961 attgttttgt tttcgccatt gttcaagaat ttcatgaact tctaagtaca aagcgcgttg
     4021 ctcttcattt aacaagtaag tcttatcagg ttgaaatatc tgaatatcag cccaaagtga
     4081 gcggataaac gggagaagtg ctttatcaaa aagtatctga ttttcttcag atatattcag
     4141 agataagata agtttttgac tgagctcaat caccaaaggg ttatctgcta catggttttg
     4201 ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta tcgcatagat aatttctaga
     4261 gttgaataga aagtagatat aaaggcattc atcttcgtca tgacataaag agaaagaaag
     4321 ttgctctaat cctttctgta ttagctgaaa caagggagta tgtcgggcaa ctcttttttc
     4381 ttcctgacta aaatatattg ggttagtctt agatctctgc aaaccgatag cgatacctaa
     4441 taaaataaat tgtttttcaa ctgtagggta acttcttgaa aaaaaatgtt tttctacata
     4501 atcaatcaat tgttgaccgc tttgaataat tgatttattt agctggatca actctgtccg
     4561 atttgtctca tagattatcg ttagtaacaa acttcgataa tccttttcag gaatttcaaa
     4621 atggccatca gcatttaagt actgcatttg tttaaaaaaa ttaatcaact catttcgtaa
     4681 gttgtaaact ttgcttaacg aaatgaaggt agtatcagct atattactcc aattgtgttc
     4741 ccctgataaa taatgtccta gtacattgaa aaagtcagag cgctgataga tgagttgagt
     4801 gagtgccaac ctcgaaaacg aagagtcgaa tgcgactgta aagctacttc tagatttctc
     4861 cactgaaata atttgctgat ggagtatgtc ggtaatgcat tccaagtcgt tagtgacagt
     4921 gagtgggcaa acgttcaatt gatctgctat ttcgcgaatc tgaatggtcc ctttttcgtg
     4981 taattgctca caaatgtaaa cttgacgaaa aatacttttc tccagatagg tttccaacat
     5041 agtactcctc ctagtcattt tatacata
//
LOCUS       DACDHJ010000160         5054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDHJ010000160 DACDHJ010000000
VERSION     DACDHJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.163"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..619)
                     /locus_tag="IV223_002122"
     CDS             complement(<1..619)
                     /locus_tag="IV223_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8712381.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGL"
     gene            complement(629..1444)
                     /locus_tag="IV223_002123"
     CDS             complement(629..1444)
                     /locus_tag="IV223_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8712382.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1706..2149)
                     /locus_tag="IV223_002124"
     CDS             complement(1706..2149)
                     /locus_tag="IV223_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8712383.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2283..2621)
                     /locus_tag="IV223_002125"
     CDS             complement(2283..2621)
                     /locus_tag="IV223_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8712384.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2609..2986)
                     /locus_tag="IV223_002126"
     CDS             complement(2609..2986)
                     /locus_tag="IV223_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8712385.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3210..4403
                     /locus_tag="IV223_002127"
     CDS             3210..4403
                     /locus_tag="IV223_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8712386.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4569..4991)
                     /locus_tag="IV223_002128"
     CDS             complement(4569..4991)
                     /locus_tag="IV223_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8712387.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1528 a          964 c          873 g         1689 t
ORIGIN      
        1 tcaaaccaaa tacgatagaa ggacctagta gaatacacca ttggggcttt acgaaaaaga
       61 agagaaacaa acttgtcaat agtaaaaaat ctagtaaact cacataccat tttttcaacg
      121 tatagtagaa cgctatccgt agaacttgat ttggtttcga cgctggattt ctcacctgaa
      181 aataccagta gttgatgcta aaggaaactg ataaacaagc aagtaaaaca aacaacggaa
      241 tcaaccattt tccataagaa aaacgtataa aaaagaaaca atcaaaaatc attatcacac
      301 taactatcca agcaaatagc caataaaaaa atccctttag ccaaaattgg cgaaacgtct
      361 ttatgaacaa acgtactggt gaaaaacttt tttcttttgc taattcttct gtcataccta
      421 tcatcacgat gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc
      481 ttccatctat cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac
      541 aaaggctact tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt
      601 caaaacgctg ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt
      661 ctacaaaata tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg
      721 aggcagccat gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta
      781 actggattgt tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc
      841 atgtattaac gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa
      901 tgatcttgcg atagattccc cactctgata agccatcgat ccgcgcactc tcacataaag
      961 aatctgggat actactgtag tattgcttca acaagaacac accaaaagca ttgaacgctt
     1021 gtaatagcac caacgaccac aacgtatatg ttaaacccac ttttctcatc ataatgaact
     1081 gcgggatcat ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa
     1141 tatcgcgccc tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca
     1201 actgaatcaa cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag
     1261 gaatacgaga ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa
     1321 tcggaatcgt gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag
     1381 gaatgatcgt caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg
     1441 gcatgttttt tgaacgaatt tttgcttttt ttctttcatt tcgaacacct ccaaagttat
     1501 aatcttcatt tctttgtaat gattacaatc cagaacaacg gttattatag ctgattcatt
     1561 tccgttcttc ttatgtttgt aagcgcttat ttaacaagtt atgtaaatct ctttattttt
     1621 tttgatttaa aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc ttttgtattg
     1681 aaatttcttc gattagagat acttattact gttcaaaagc ttctttgaaa cggtgaatca
     1741 attcattgac tacaggtgat aaaatccgat tctttttcca gactagcatg cagttcgttt
     1801 caatcattgg gtcaagcgga acgaatgtca ctcgctttga aagatgactt acagcacctt
     1861 ctatgactaa agcagcacct acctgattct caattagcgc aaatacattg aaaatcaaat
     1921 tgtacgttcc tgtgatcagc aactgctcct tttttatgcc taaccagtca gcaaacattc
     1981 gctgtatttc tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta
     2041 tcacgtcttt ttttgctaaa acagaatttt ctaaaatgag caatccccat ttttcctttc
     2101 ttggtaacaa aatcgtttcg tatttctctg tatctactgg atctagcaaa atcgctaaat
     2161 ctaacaggct tttttctagt cactccgtga tatcatcact tgttccgctg tggatatgaa
     2221 attgaacgct gggataatca cggatcaact tttctagtaa tagtgccaat gtattcgaat
     2281 tatcagcttc cacacagccg atcgtaaatc ggccgctgaa taattgtttt tttctgttct
     2341 cgaattcttg agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga
     2401 aaataacctg cttctgtcaa gatcattttg cggttttcgc gtacaaacag ttctgttcct
     2461 agttcctctt ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag atgtagaact
     2521 tctgcggctt gagagattgt tccttcttct gcaattgtcc aaaaatattt tagtaagcgt
     2581 aactccatag cgatgctcct tgattttttt acttgataca ttccatttta tttctagata
     2641 acacacgatc atatttcttg atttttcctt gtaaacgttc caaacattct tgcatttcag
     2701 caattttttt cgtcaaaagt tcactttctt caatcaaaag cttacgccgc tcaaatacag
     2761 ttgcttctcc ttcgctagat aggcgaatat attcaatcaa agactcgatc gataatccgg
     2821 catttcgcat acagatcgta aagtcgaccc attttaggtc ttcttctgta taatcacgga
     2881 ttccgccttt tgtccgatta actggtggaa tcaacccgat tcgttcataa tagcgcaatg
     2941 tatcggccga aatgcccttt tcttcactga cttgtttgat attcatctga tcactctttc
     3001 tcttaatttc ttgccacttt acttatgtat tttatttatt ctacaacttt aagtgtactc
     3061 caagtcaata ctcatttcta ttaaaactaa gctggattat ctatatcggt tgtttgtgta
     3121 ttatttcaaa taaaaaagtc ttgttttttt attctttttt ggatatactt ttaacaattt
     3181 agggaaattt taggaaaagg ggtataaaaa tgacaacgta tcagcgaaag ttattattat
     3241 cgacatcggc gggaattgct ttagagaata tggatattat gtttttagct ttttcattat
     3301 cttcaatgat tacttctttt catattagtg gtacgcaagc gggtttcatt tcaacgatca
     3361 ctaatttggg gatgctcatt ggaggtattt tttttggcat catagctgat aaatatggac
     3421 gtgtgaaagt attttctcaa acggtccttc tgttttcgat tgcttcttta ttgatgtatt
     3481 ttgcttcaaa catctacttg gtttatctct ttcgctttat cgcaggtgta ggagcgggag
     3541 gagaatacgg ggcgtgtatg tccttgattt ccgaaagttt ttcaaaaaaa caaattggac
     3601 gagcttcttc ggttgctggt attggtgcgc aggtcggtgc ggctttggct gcgattttag
     3661 ctgctgtgat cattccaacg tttggttgga agatgttgta tgtcgtaggt gtactgccgg
     3721 tgttgatggt cttgttcatt cgtcgcggct tgactgaacc aaaggaattc aaatctgtac
     3781 aatctgccga aaagaaaacc aaacttagca gactgtttga atcaaaacat cttgcttggc
     3841 aaacatgtgg ccttagtatc atggtaactg tccaaattgc cggctatttt gggttaatga
     3901 attggctgcc ttcaattatg caaaatcgat tagggctttc ggtttctggg tcttcattgt
     3961 ggatggtcag tactatttta ggcatgtctt tggggatgat gacttttggc ataatcatgg
     4021 ataaattggg gcctaaacta gccttttctt tgtttttaat ttgttcatcc ttatctgtct
     4081 atctacttgt gttagcccat agccagtgga gtttggtagc agccgctgtg atagtcggct
     4141 actttatcaa tggcatgtat ggtggatatg gcgcagtgat tagtagtttg tatccaacag
     4201 aaatccgcgc gacggcgaat aatttcatta tgaaccttgg acgagctgtt ggtggttttt
     4261 cttctatcgt catcggtttc ttgatggatc attatagttt aactgccgta atcttgtttt
     4321 tgagcggtat ttacctagtc agtctgctgg tcttgacaac tcttactggt gttacagcat
     4381 taagggaaag cttgcagcat tgatagttga gtctgtaaat gacaagaaag aggtgtggca
     4441 aaagcttgca tctgcggtaa taagaccagc caaatcaaaa acatgagaca ctgtttccgt
     4501 ttggctggtc gtattacttg atgcagggca gctttttcaa ctgctaattt ccttctatct
     4561 ggcggctact attctttgac gtccgtcatt tcaaagtact ttactaataa cttagcataa
     4621 cgaaaaactg tggatacact cgtatattga gcgtctgcat aatcttccaa atttttctca
     4681 ggatgttgat acatgaattc aatcaaatga taattatatg attttctgta gaaatgaaga
     4741 taaataatgt agattgggaa gttctcctgc ttttccatag aaaagcgccg attattctca
     4801 ttcttcaaaa tgaccttatc aggatatttt tggaacaatt cttctaaatc tgttagataa
     4861 ttcaagatag acttacttga taccttcaat tcgttttgta gttcttctat tgaacaccaa
     4921 cgttctgttt caagcagtaa atgaatcatt ttaattttct tttgagaaac ttttgttaaa
     4981 aaagtctgca tattttctcc tcctgtatct actttactat actacataca agaaaaaaaa
     5041 cggatacaaa tatc
//
LOCUS       DACDHJ010000161         5012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDHJ010000161 DACDHJ010000000
VERSION     DACDHJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.164"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            87..1802
                     /locus_tag="IV223_002129"
     CDS             87..1802
                     /locus_tag="IV223_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712388.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1805..3568
                     /locus_tag="IV223_002130"
     CDS             1805..3568
                     /locus_tag="IV223_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712389.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3608..4144)
                     /locus_tag="IV223_002131"
     CDS             complement(3608..4144)
                     /locus_tag="IV223_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8712390.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4467..4889)
                     /gene="mgsA"
                     /locus_tag="IV223_002132"
     CDS             complement(4467..4889)
                     /gene="mgsA"
                     /locus_tag="IV223_002132"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8712391.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1501 a         1011 c          974 g         1526 t
ORIGIN      
        1 tctcttttca ttctaaattt cctaagctac aatgtggtag tacttgtttt ttcaattgtg
       61 gacaaataaa taataggagg aatcatatga ttcagttatt acgttatgca aaagactatc
      121 gtaagcaaat cattttgggc ccgttcttca aatttcttga agctgtcttc gaacttgtcc
      181 tgcctttaat gatggcttcc ttgatcgata atggactaaa aatgaatgac cgaggaaaaa
      241 tcattgaaat gggcttatgg atggtcgcta tgtctgtgat tggtctgatt tgtgctatca
      301 tttgccaata ctatgcttca gtagcttcac aaggatttgg tacagagtta cgtaatcagc
      361 taatcaaaaa aatcaatact ttctctcaca acgagttgaa tcattttgga acagatacct
      421 tgatcacccg aatgacaaac gacatcaatc agcttcagct agctttagca atggtcattc
      481 gtttattgat cagagcaccg tttttaagta tcgggtcagt ggtcatggct tttgtgatcg
      541 actgggaagt aggtctgttt ttcttagcat tattgcctat tttctcaatc attttatttt
      601 ttatcatcaa aaaaaccgtt cctctctacc aaaaagtgca agaaaaacta gatcagctca
      661 atgaacacgt tagtcagaat cttagcggtg ttcgtgtcat ccgcgcattt gcgcagacaa
      721 aaaaagaaac agagaagttc gataaaagca caaatgatct agcgaacaat tatttacgtg
      781 tttccaatat ctctgcccta ttatcacctg ctacaacctt gatcatgaat gtcgggatca
      841 tctgtttgct gacagttggc gggatcaaag taaatattgg tagcttgcaa caaggacaag
      901 ttcttgcttt gatcaattat atgaatcaaa tgcttttggc actgatcgtt gtttcaaacc
      961 tcgtcgtgat cttcacacgt gccgaagctt caggaaatcg ggtgaaagag gtgctagaca
     1021 cagaaaattc gattcttgaa gctgaggtca gtactactcc cgatttttct tctgaagcaa
     1081 ttatcgcttt tgaccatgtt gacttccgtt atactcctga ttctggtctt tctctccaaa
     1141 atattacatt tcagctgaag cgaaatacag tccttggaat cattggtcca accggaagcg
     1201 ggaaaacgac tttgacacaa ttgattccgc gtttctatga tgtcagttcc ggtacagtta
     1261 taatggacgg aatggatgtt cgttcttggc agctggatca tctccgtcag caaattgctc
     1321 ttgttcccca aacttctgta ctatttaccg ggactattcg tgaaaacctt caatggggta
     1381 agaaagacgc cacagaaaaa gaatgttggg aagctctgaa aatcgctcag gcagaagaat
     1441 ttgtccgtca gcttccaaat ggtttagata caaaagtcat ggaaaatggg aaaaatttct
     1501 ctggcggaca aaaacagcga ctgacgattg caagagcttt gattgccaag ccagcattac
     1561 tgattttaga tgactcttta agtgcgttgg attatcaaac agacttggat ttgcgtaagg
     1621 cgctacagac ttatcttggc agtacagtga ttattatctc ccaacgtgtc cgctctattc
     1681 aagaagcaca tcatatctta gtgatggatc aaggtaagat tgctgctcag ggtacccatg
     1741 aagaattact tgctcaatca gcagaatatc aggaaatcgt cgcatcacag gaggaagaat
     1801 agttatgaaa gtaaaaaaat tatccttgca gacgctccgc cgattcattc cttaccttac
     1861 tgcctatgca aaagacattt ggctagctgt gatactagga atcatcaatg ggattgcaac
     1921 agttgtgatg acgttacaaa ttggtcaaag tatcgatcaa atgattggag aaggacaggt
     1981 ccgcttcaat cgactgttcc atttacttat tctattcggt ggtatcgttt taatcaacgt
     2041 agtcagtcaa tggctcatcc aagtgttaag caatcggtta gcttacttat ccgttgcaaa
     2101 attaagaaaa gatgcatttg ctcatctgaa tcgattgcca cttcgttatt atgatcaaaa
     2161 ctcccacgga aatatcgtca gtcgatttac aaatgatatg gacaatattt ccattgcaat
     2221 ttctgcggcc ttcaatcaat tattttctgg agtggcaatc atcattcttt cgtttatttt
     2281 tatgatgcgt ctaagtccac ttctgacatt ggtcgttctt tgttctacac caattatttt
     2341 ccttgtgagt tggatcgttg ctcgtgcttc acaaacaaat ttcgatagac agcaaaggat
     2401 tattggggat atctcgggct ttgtgaccga acgaatcggt aaccagaaaa tcgtgaaagc
     2461 ttttcagcaa gaagaagcta gtcaacagca atttgaactg ctaaatgctg atctatatga
     2521 aaaaggacaa cgggcacaat tttcttcttc cttgacaaac ccgtcttctc gttttgtaga
     2581 tcaccttgct tatttatcga ttggtttagt tggcggccta ttggcattga agagtggttc
     2641 gtcaatcact gttggtgtga tttccagctt tacgatctac tcaagtcagt tttctaaacc
     2701 atttatcgaa ctttctggaa tcacgacaca actgcaaacc gctttttctg gtctagaacg
     2761 agcatttgat ttgatcgatc agccagaaga aaagcctgat ttgccagatg cctatgcctt
     2821 ggaacccaaa aatatcaaag gaaaagtctc ttttgatcat gtttcttttt cttatgaccc
     2881 caagcagcgt ttgatcgaag atttcaattt tactgcttat ccaggtcaaa ccattgctat
     2941 agtaggaaaa acaggggccg gaaaatccac acttgttaat ttgctgatgc gtttttatga
     3001 cacaacaaaa ggaacaatta cagtcgatga ccatgacatc cgctctatcc aacgtgatac
     3061 attacgtaaa agttttggaa tggtgttaca agatacttgg ctatttgaca gtacattgcg
     3121 agaaaatctt cgttttggga atccggaagc aagtgacgca gaaatcaatg atgccctaaa
     3181 agcctcttat atgtatgaat ttgtagaacg tttgccggaa aaattagata ctaagattgg
     3241 cggtcaaggg ttaaagatat cagatggaca acgccaactg ttgacgattg ctcgaacgat
     3301 gatcagtaat ccccctatgt tgatcttaga tgaagctacc agctcagtcg atacattgac
     3361 agagaaaaaa atccaacaag ccttcttagc aatgatggaa ggaaaaacca gttttgtgat
     3421 tgcccatcgc ctgtccacaa tcaaaaatgc ggaccagata ttagtgatgg atcagggagc
     3481 tattgtcgaa atgggcaccc atgatgagct gcttcaaaaa gatggttatt accgacggct
     3541 ctatcaagca caattcacag cacaataaaa acaagcagtc ttctgggatg ggaagactgc
     3601 ttgtttttta tttagcgtgg atcgctaaac ttcttaaaat ttttttgtaa ggaatactac
     3661 gtcctacgta ataaatggca attgattgaa tcggtaacca tattactgtt tttaacaggc
     3721 ggggcgccca aacgacccag ctgaacaatg gcacatggta catcaacgca agccataatg
     3781 gtgtcaaaaa taagttgatg atcagcgtga cactaagcgt agccagaatg gcatttttcc
     3841 aagtgatctc ttttcgatag aaaaagaatc cgtagattgc tccttcaata aatgcattca
     3901 atgtaaatcc aataaagaat gcagatttcg agaagagggc cattcctact aaatcagcta
     3961 aaactgcgcc tatccctgac caaaaaggac caaaaagaat gcccatgatc acttgtggaa
     4021 taaatgtgac actgatttta ataaatgggg tctcgattgc taccagtcgg gacaaagtga
     4081 ccatcaatgc aatcaataag cccataatag caatcatacg tgcgtctaac cggtgtttct
     4141 tcatttttag catctccttt aagagctgct acatcgtatc atgcagcgaa tgcgaaaata
     4201 aaaccgcgaa cattacgtcc ttcgtccaag aggcaacatc ccatcctctt agcacttaac
     4261 gctttatttt ctactctgta ttatttttgg gatcttttcc cacagaaact atagcatatt
     4321 ttcacattta ctgcaaatac gaaaaactaa tattattgtt tatattatcc aatacgttcg
     4381 tattatatcg catattatca gtttgtttag cttttatagc tacatatagg cgaacattaa
     4441 acgttatttt tccatacgct tatccatcaa aatgaaagtg gatgcttgtc tatttcatgg
     4501 acgattcttc gaaaatctag gaagccggct tccagtcctc ttagtagcac ctcagctgtt
     4561 ccgatattcg tagctaacgg aatctcatag acatcagata aacggataag tgctgttaca
     4621 tctggttcat ggggctgtgc agccagcggg tctcgaagaa aaatcaccaa atctaactga
     4681 tcctccgaaa tcatggctcc aatttgctga tccccgccta acggtccgga tttaaaccga
     4741 tggatcggca agccggttgc ttccgtgatc ctctgcccag tcgtcccggt agcaaaaagt
     4801 tcatgttttt ccaatattgt tttatacgct gttgctaatt tcaccatcat aggttttttt
     4861 cggtcatgag cgatcaaagc gattttcata agagacactc cttttgatat tgttaacata
     4921 cttatttact ccagtgtatc agtttttatt tcttatggat atttattaaa gcttaaaact
     4981 agcacattga caaaaaaaag aaaaaattct tg
//
LOCUS       DACDHJ010000162         5009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDHJ010000162 DACDHJ010000000
VERSION     DACDHJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.165"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IV223_002133"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IV223_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8712392.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IV223_002134"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IV223_002134"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8712393.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IV223_002135"
     CDS             2591..3376
                     /locus_tag="IV223_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8712394.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IV223_002136"
     CDS             3389..4240
                     /locus_tag="IV223_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8712395.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IV223_002137"
     CDS             complement(4364..4816)
                     /locus_tag="IV223_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8712396.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1696 a          818 c         1043 g         1452 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagt
//
LOCUS       DACDHJ010000163         4992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDHJ010000163 DACDHJ010000000
VERSION     DACDHJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.166"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(75..764)
                     /locus_tag="IV223_002138"
     CDS             complement(75..764)
                     /locus_tag="IV223_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8712397.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(809..1573)
                     /locus_tag="IV223_002139"
     CDS             complement(809..1573)
                     /locus_tag="IV223_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8712398.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1601..2299)
                     /locus_tag="IV223_002140"
     CDS             complement(1601..2299)
                     /locus_tag="IV223_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8712399.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2311..3090)
                     /locus_tag="IV223_002141"
     CDS             complement(2311..3090)
                     /locus_tag="IV223_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8712400.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3106..4050)
                     /locus_tag="IV223_002142"
     CDS             complement(3106..4050)
                     /locus_tag="IV223_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8712401.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4096..4875)
                     /locus_tag="IV223_002143"
     CDS             complement(4096..4875)
                     /locus_tag="IV223_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712402.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1307 a         1055 c          866 g         1764 t
ORIGIN      
        1 cagttattac ttttgaaacg ataatatgaa catgtgtaat cttttttatt aacaatccat
       61 cattctatcc ctaactaata tgctccattg ggcttgacaa taacataaaa cgtttttaaa
      121 atcaacctca agtcgtacat taaacttcga tgactaatat actctaaatc caactcaacc
      181 attttatgga aactaacatt atttctatca ctaacttgcc acaaacctga acaaccaggt
      241 tttacataca agcgttgaag atcatatctc gtgtactcag ctacttccct ctccaaaggt
      301 ggccgtggac caattagact catatcgcct tttaaaacat tccatagctg tggcaattca
      361 tcaatgcttt tggcacggat aattttacct attttagtga cacggggatc ttctttcatc
      421 ttgaacattg caccttctat ttcattttga tcaagtaatt cttccaattt ttcttcagca
      481 tctaaacaca tcgaacggaa tttatacatt ttgaatgttt ttccattttt accaacacgt
      541 gtatggcaaa aaaatatcgg acttcctggc tcttcttttc tgattcgata agctacatag
      601 aacatcaaag gagaagctaa aatcaaacca ataatgcttc ctacaatatc aatcattctt
      661 ttaaaaaata aatagatata tcgtttttca actttttctt tatccaacca aacttgatta
      721 ggatgtactt cactaatacg agataataaa tcttcacttt ccatactttc cctctctcct
      781 catttccttt ttatctacga ataaagaact aaaataaacc aaacttcttc ttttttacct
      841 cttcatagac tggataactg atttcatccc cgttgatcag atcctttgcg acttgtttca
      901 tctgtactac tttttcctta ccaaagtcct tagcaatctg ctcatagcac tcttttaagt
      961 aaaaggtacg cttagagacc ccatgggcat cagaagcagc catctgcacg agattatgtt
     1021 tgaccattgt tttggctgtt ttctgaatgt ctttgccaaa tacaccaaca taacttggcg
     1081 cagtaatttg ggccaaacat cccatatcta ggaacggaag caaatgattt gggtcttctc
     1141 gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt tctaacaact
     1201 caaaaaaaag ccattctgca tataacggca cttccagtgt cggaaattcg atcagaagat
     1261 atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg atgagcgttc
     1321 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt ttctctaatt
     1381 cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     1441 tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca
     1501 ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg atatggcaat
     1561 gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg tagtaataac
     1621 cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt gcttgtacca
     1681 tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt tcacgtacga
     1741 ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg gctactactg
     1801 gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg atctgatcca
     1861 ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta ggcaaaatcg
     1921 acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat gaactaagtg
     1981 ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg ggtttacgta
     2041 aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact gcaatgtttg
     2101 ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     2161 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     2221 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt gctgcttttt
     2281 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     2341 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt tacggatacc
     2401 agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga atcctaatgc
     2461 atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata agaatgccag
     2521 acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt tattatttgg
     2581 cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat cgacattcat
     2641 cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct gttgagcagt
     2701 cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg actgattgac
     2761 ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg tttctaaacg
     2821 atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg tattgatcat
     2881 ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca gtgtcacgat
     2941 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
     3001 ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta atgtttcaaa
     3061 aatttcttcc aagttgattg tctcttccat tttttctcca ctttcctatt tattctctag
     3121 attcatttga tcttttaatt ctttttttac acgatcaagt tcttgatcac tgactttttg
     3181 ataggagata ttatcttgca taaagccttc tccttgcagc tgatcttgct tgatcgtacc
     3241 aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca tgtctgtttt
     3301 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttaaactcaa
     3361 tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac catagtcacc
     3421 atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtgat
     3481 tttaccgatt gtaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
     3541 accaccaact gcatcaacaa gttcctttag ccctttcata ttgatagaga catagtgatc
     3601 gattgggatg tctacatatt tttgtacggt atccatcgcc atggcagctc cgccgaatgc
     3661 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatcacg
     3721 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actatggcaa ccatcatcgt
     3781 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatcccca tcaatagaac
     3841 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
     3901 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
     3961 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa atattttttt
     4021 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
     4081 tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag caacagtttg
     4141 tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta tttgactgag
     4201 cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct tcaacatcgt
     4261 atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga atcctaaata
     4321 ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt cacgtacacg
     4381 agcgacatct gcacttgtca ccttttcttt gatgatgaca tcgtctacag cttgctgcca
     4441 gtttgataca tttttagtaa acaaatcgtt gacagctgtc tggatcttca gttttgattc
     4501 tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa cagcttcttt
     4561 aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacat aggatagctt
     4621 atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaaa ttttttcttt
     4681 tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt tttgattggt
     4741 gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat tgatatggta
     4801 accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca atgacttgac
     4861 gaatggcttt tccatagatt cactcctaag tattgatgat agataaatta agatataatt
     4921 cacttacata aaaatgacag ctttttattt taaaaaacat aattaaacta atatattcac
     4981 agaaatattt gt
//
LOCUS       DACDHJ010000164         4992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDHJ010000164 DACDHJ010000000
VERSION     DACDHJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.167"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IV223_002144"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IV223_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8712403.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IV223_002145"
     CDS             complement(2066..3064)
                     /locus_tag="IV223_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8712404.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IV223_002146"
     CDS             complement(3290..3943)
                     /locus_tag="IV223_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8712405.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IV223_002147"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IV223_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8712406.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1481 a         1032 c          762 g         1717 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttttat
     4981 gttttttatt tc
//
LOCUS       DACDHJ010000165         4948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDHJ010000165 DACDHJ010000000
VERSION     DACDHJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.168"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..558
                     /locus_tag="IV223_002148"
     CDS             73..558
                     /locus_tag="IV223_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8712407.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(662..1255)
                     /gene="clpP"
                     /locus_tag="IV223_002149"
     CDS             complement(662..1255)
                     /gene="clpP"
                     /locus_tag="IV223_002149"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8712408.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1435..1962
                     /locus_tag="IV223_002150"
     CDS             1435..1962
                     /locus_tag="IV223_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8712409.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2053..2790
                     /locus_tag="IV223_002151"
     CDS             2053..2790
                     /locus_tag="IV223_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8712410.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2794..3711
                     /gene="miaA"
                     /locus_tag="IV223_002152"
     CDS             2794..3711
                     /gene="miaA"
                     /locus_tag="IV223_002152"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8712411.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3713..4942
                     /gene="hflX"
                     /locus_tag="IV223_002153"
     CDS             3713..4942
                     /gene="hflX"
                     /locus_tag="IV223_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8712412.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1686 a          849 c         1110 g         1303 t
ORIGIN      
        1 aataaatata tgctatgata cctttattca ataggattag tttctacaaa aaatgaaagg
       61 agaaaaataa atatggaacg gtttatagca tcaaatgcca cagtgattgg tgatgtgaca
      121 ttgtcagaag atgtgacgat ctggtatcaa gctgttttac gcggggacag taattggata
      181 aaaataggac aaagaacgaa tatccaagat ggtacaatca tccatgtcga tcatgatgca
      241 cctgtagata ttgcggagaa tgtgacggta ggacatcagt gcatgctaca tggctgtaca
      301 atcgaaaaag gggcgctgat cgggatgggg accacgatat tgaatcatgc ggttattggt
      361 gagaatagtt tgattggtgc tggatcatta gtaacagaag gaaaagttat tcctcctaat
      421 gtcttggctt ttggtcgccc agctaaagtg atccgtcctt tgacaaaaga agaaatacaa
      481 aaaaacaaag aaaacatcca gcattatgtc gagataggtc ggatgcatgc gcaaggaaaa
      541 ttcaaggagc aaagataggc agtagcatcc ataaaataaa aaaggatggt cagtttgagt
      601 tgaccatcct tttttatttg gtttagataa gagagcttaa aaaagcttcc tttgaaacag
      661 attaatttaa tgaagaactg ttttccatga cttcatcgat caatccatat gcttttgctt
      721 gttcagctgt catatagttg tcacgatctg tatctttttc aatcacttca agcggttgtc
      781 cagtgcgttc agctaaaatt ttgtttaagc gttcgcgtgt ttgcaaaata tggcgtgccg
      841 caatttcgat ttcagttgct tgcccttgag cgccgccaag aggttgatgg atcatgatct
      901 cagcatttgg taaagcaaat cgtttacctt ttgtaccagc tgtcagcaag aagcttccca
      961 ttgaagcagc catacccata acaattgttt ggacatctgc tttgacaaag ttcattgtgt
     1021 cgtaaatagc catacctgct gtaactgaac caccaggaga attgatataa agatagatat
     1081 ctttttcaga atcttgagcg tctaagaaaa gcaattgggc gatgatcgaa ttagcaagat
     1141 catctgttac ttggccgctt aacatgatga tacggtcttt caataaacgt gagtagatat
     1201 cgtaagctct ttcacctcgt gaagattgtt caatgactgt aggaattaaa ttcataatag
     1261 agttcctcct tgatttcgtt tagaaatagt gtaccataat ttagtttcta atacctaaaa
     1321 tgtagtatac atttttggtc aaaaaaggtc aaataaaaaa cttaggcaaa ttagtcgcat
     1381 aaacccgctt tatggtatga taagcagaga aacaaaacaa ggaggaacaa cagattgata
     1441 gttggtatta ctggccttat cggagtgctg acaggtgctg tagttgtcta tatcggcatg
     1501 atactgcaaa tgcgtcactt ggaaaataaa gaacagcgta gacgtgaatt ggaattgaaa
     1561 gtcaaagaaa tcgagacgct gaatcaatta aataaaaaga tcaacgagat cttgcaaaaa
     1621 cggacaacgc tgatggaaga atatgtcagc tttgatgcat ttgatgattg ttatatcacg
     1681 attgacgatt ttgcgtatct ccaatcattt tcagcgcaaa atagttttta tctgccaaat
     1741 tatattttag acgagctatt taagaatatc tcccatcgaa aagtgatttt atctccagat
     1801 gaaacaatga agattggtgg atatgcttat aaaggcggac gcgtgatttt agagaatttt
     1861 acggatagtc tgactgaaat gatcaatgaa aaaaaagctc agttgaagac tatgacgcgc
     1921 cagccgattg gtttcttctc gaaagatgat gtttggagct gatattttga agcattgccg
     1981 ttgcacatga gagcgtggta atgctttctt tttttcttaa tgatacaata gaactgaacg
     2041 gagtgaagaa agatgttaca aatctatgcc catcgcggaa gcagcggcac ccacccagaa
     2101 aatacgcttc ctgcatttgc agaagctgta cgagtaggtg cagatggtat cgaactagat
     2161 gtccatctaa gcaaagatgg ttatttgatc gttatgcacg acgaagaagt ggaccggacg
     2221 actaacggta aagggctgat tcgagaaaaa acattggaag aactcaaaaa attgaatgca
     2281 gggggcaaat tcagcaaaga atttccagca gctaaagttc ctgaattgag agaagtgtta
     2341 ggactgctcc tccaaaaaaa ttaccgaggg atgctaatga ttgaaattaa aactgatcag
     2401 tatgaatatc ccggtatcga agaaaaatta gccgaaacat taaaaagtag agattggcct
     2461 tttcaacatg catattgcag ctttcatttg gattctttgg aaaaaatcgc attactggaa
     2521 ccggagatac agctggactt attgatgagt acttctgata aaaagatcca actagcagca
     2581 gaaacccctt atatagaagg agtacatcca aaaaatactt ggttgttcga acatccagtc
     2641 caggcagctg attttccaaa agcaattcgt gcatggacga ttaacgatga agaagaaatg
     2701 gccaaatgtc aagaactcaa aatagatgga ataataactg attttccgga gaaagctctt
     2761 cggtggaaac aatcaacgaa aagtaggtaa aatatggaaa aagtattagt gatcgtcggt
     2821 ccgacagcag tcggtaaaac agcgctaagt gtggaactcg cacaactatt tggaggagag
     2881 atcatcagtg gtgattcatt gcaaatctat aaaaatctgg atatcggtac agcaaaaatc
     2941 aaagaagaag agatgaataa tgttccgcat catttgattg atgtgatcga acctactcaa
     3001 cgttactcag ccgctgattt tcagcattca ggtcgacaat tgattaccga gattgcgaac
     3061 cgagggcacc tacctatcat agctggtggt acgggtctct atatccaatc ccttctatat
     3121 gattatcagt taggagcgac agaagaagcg aatcctgaga ttagaaaaaa gtatgaaacg
     3181 ttagctgaaa caattggttc ccaagcatta tggaatcagt tgaaagaaaa agacccggca
     3241 gctgctgaga aaatccactg gaacaatcaa cgcaaagtga taagagctct ggaagtttta
     3301 gagacgacag gacatagtat cacggcacct aaagaagagc caaagcaatt gtatgattac
     3361 ctcatgattg gattagatac gaagcgtagt attctttatg aacgaatcaa tcaaagagtc
     3421 gatttgatgc tagcagaagg tttagtagca gaagcgcgta aggtgtatga gcttggagaa
     3481 gtccaggcca gtcaaggtat tggatataaa gaatttttcc cttattttga agggaaagaa
     3541 acattggaag aagcaacaga acaagtcaaa ctacattcga gaagatacgc gaaacgtcag
     3601 ttgacgtggt ttcgtaaccg tttggatgca cagtggtttg atcttataag tgagccaaat
     3661 caaaaaagtc agattgagca aacaatcaaa aaatggctgg aggcggaata aaatggaaga
     3721 aaaagtgatc atcgtagggg tagaaaccga agcaaaccag cgttactttt cagagtcaat
     3781 ggaagagcta gttcagctga cgaataccgc atcaggagaa gtcgtattca caattacaca
     3841 aaagagacca caagtggatc ggcaaacgat tatcggtaaa gggaaattac aagaattagt
     3901 tcaacaagcg gatgcgcatg aagcggattt gatcatcttt aatcatgagc taactccgag
     3961 acagagccaa ctgattaccg atgctgttgg tcttccggta attgatcgcg tgcagcttat
     4021 cttagatatt tttgcgatgc gtgctcgttc gaaagaaggg aagctgcagg ttgaacttgc
     4081 tcagttagaa tacctgctgc ctcgtttagt agggcaaggg aaaacattat ctcgtcttgg
     4141 cggtgggatc ggaacaagag ggccaggtga aacaaagtta gaaacagacc gccgacatat
     4201 acggaataaa atttttgctg ttaagaaaga attgaaagaa gtagaagctc atcgagagcg
     4261 caatcgccaa aaaagaaaaa atagcgagat ttttcagatt ggacttattg gctacacgaa
     4321 tgccgggaag tcaactatct tgaatctatt gacacaagcg gatacttatt cgaaagatca
     4381 attgtttgca acgttagatc cattgactaa gagatggcgt tttgctgaag gatttgagat
     4441 tactgtaacg gataccgtcg ggttcatcca agacctgcct acgcaactga tcgatgcttt
     4501 ccattctaca ttagaagaaa gtcaaaatat ggatcttttg ctacatgtag tcgatgctag
     4561 ttctccagat cgtattttac aggagcagac tgtcttaaaa ttgatggatg aactgaatat
     4621 gaaagaaatg ccgatactga ctgtttacaa taaggcagac cagatcgacc cagcgatgtt
     4681 cacgcctacg ctattcccaa atgtattgat ttctactcag acgaaagaag gtaagatggc
     4741 gttgatcgaa gcaatcaaac gtcagctgat ggaactaatg accccttata cactttctgt
     4801 gccaagcaac gaaggacaga aattaagcga gttgagaaga caaacaatgg ttttggacga
     4861 agcgtacatg gaagaaacaa acgaatatga agttaaaggg tttgcaaaaa ccaattcaaa
     4921 atggttaaga aaaaaccaat aaaatttt
//
LOCUS       DACDHJ010000166         4948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDHJ010000166 DACDHJ010000000
VERSION     DACDHJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.169"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(174..1784)
                     /locus_tag="IV223_002154"
     CDS             complement(174..1784)
                     /locus_tag="IV223_002154"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8712413.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2977..4317)
                     /gene="glnA"
                     /locus_tag="IV223_002155"
     CDS             complement(2977..4317)
                     /gene="glnA"
                     /locus_tag="IV223_002155"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8712414.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4345..4737)
                     /locus_tag="IV223_002156"
     CDS             complement(4345..4737)
                     /locus_tag="IV223_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8712415.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1465 a         1060 c          805 g         1618 t
ORIGIN      
        1 tcaagaagtt ttttgcaaaa aataaaatat ccaatagcgc tttaatcgaa aaaagtcttt
       61 tgctttctat cttttttcct acaaataata acaaatcaga aaaagagaaa agtgacaaga
      121 aaattgctta tccccttctc ttgctacttt tctctttcta tcaagttcaa aaattatttt
      181 gtttcgtgat ctgctaatga tgctccgaca aatgctttga ttaatttttg cgggcgattt
      241 ggacgagaaa tcaattctgg gtggaattgg catcccacaa agaatttttt ctctggcagc
      301 tctacgattt ctactaaacg gttatctggt gagacacctg aaaagactag cccttgttct
      361 tcaaacaatt gacgatattt gttattgaac tcatagcggt gacggtgacg ttcttgcaca
      421 acatcggctc catcatatgc ttcagccgtc ttcgttcctt ttttgatttt acatgggtat
      481 aatccaaggc gtaaagttcc acccaaattc tcgatgtttt cctgatcagc cattaaatca
      541 ataatattgt ttgctgtatc tggattcgtt tcagctgatg acgcatcttc taatccaacg
      601 acgttacgtg caaattcgac acaagccatc tgcataccaa gacaaatacc taaaaacgga
      661 acgtcatttt cgcgagcata acgaatcgct tcgattttcc cttctactcc gcgatcacca
      721 aatccaccag gaaccaagat tccatctgcg tctttcaaac gttcttctac attttctctc
      781 gttaattctt gtgctttgat ccaatcgatc tcgatgtctg aattataggc gaaaccagaa
      841 tgtttcaatg cttcaactac tgagatatat gcatctggta actcgacata tttaccgaca
      901 agcgcaatcc gtactttttt cttcaagttc aagacacgtt cttctaattc tttccattct
      961 gtcatgtctg cttctggtgt atctagtttt aaatggtcgc aaacaatttg atccattcct
     1021 tgtgcttgta aaactaatgg gatggagtaa agtgtatcta catctggcga ctcgataact
     1081 gcttctggtt cgacatcaca aaattgtgcc aatttgtttt tcacgctttg agaaacaggt
     1141 tgctctgtac gtaccaccaa aatattcggt tggataccta aaccacgtag ctcttttaca
     1201 ctgtgctgcg ttggtttagt tttcatttct ccagcagctt tcaagtaagg aatcaatgtt
     1261 gtatggatat acataacatt atctgtaccg acatctgctt tcatttgacg aagtgcttca
     1321 aggaatggca aagattctat atcccctact gttcccccta cttctgtaat gatcacatct
     1381 gaatcagtca ttttagctgc tcgcatgatt ttttctttga tctcatttgt aatatgagga
     1441 ataacttgaa cagttgctcc caagtattcg cccttacgtt ctttacgcaa aacttcagaa
     1501 tagatttttc ctgtcgtcac gttggagtat ttgttcaaat tgatgtcaat aaaacgttcg
     1561 tagtggccca gatccaaatc tgtttcagca ccatcatctg ttacgaatac ttctccgtgc
     1621 tgatatgggc tcatcgtccc cggatccacg ttgatgtatg gatcaaattt ttggatagtt
     1681 acttttaagc cgcggttttt caataagcgt cctaaagaag ctgcaacaat cccttttccg
     1741 attgatgaaa cgacaccgcc ggtcacaaaa atatattttg tcataagttc aaaaactcct
     1801 ttttaattta tgtgcaggaa aagtctcaga caataaacaa agctccctat tcttcaataa
     1861 gaatagggag ccgaaatttc tgtaaacttc tgaccctcta aaagggtgcc caaggtgtat
     1921 aatacaagct gactaaggga aagtcaagta ctcaaatgaa tactatccgc ttacaatttt
     1981 ttctttttcg atatcctttg agaaattatt tcttaagtcc tttatgagtg caaaaaaaat
     2041 ttaacccaca taataagctt gatataaaca tagctaacgt taaacctaag caaatcagaa
     2101 aatcgttact gaaaaatttt aagtgaaata atagataaaa aaataaaaca aacattatga
     2161 tagataatat cccacctaac aaagaactac tttttcctac ttgcagcaac agtttatata
     2221 gcttatcgta gaaacaatct ttttctttca cccttattta cctcctttac gtaaaaaacg
     2281 tagtccacag ttctcggatt tttggcatat cttttctatt aaataatatc aaaaacaaaa
     2341 caattccact aattttttca aagatgacct ttttttcaac aaaagtgcct tgtagggatt
     2401 cattgaaggt aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa gtcgtgcctc
     2461 cttatttcta aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca
     2521 tcaaacatga gaagctgttt ttgaaattat ttcttgtgat tcggagttaa atggtaggct
     2581 cacaacctct ggataaacgg tattcaaaag ccagtttctc ggtattaagc caaatttatg
     2641 caaaaatgta gaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacgtcttt
     2701 ttcaaaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata agttcagcca
     2761 attcaaaaac aagaaaagct gtttttgctt ggctattctt attacttgtt gcagggcagc
     2821 tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa
     2881 aaacggcaaa atatgaggaa ctatttttcc gttagtttct ctaatactca ggtcaaaacg
     2941 ctgatttcac agcctctaat tcacggtatt cctgttttaa taaagttcta gatattgttc
     3001 tctttcccac tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt ttgcttcaac
     3061 aaaatttgag aagatatgtt gtcctaatgc gtcaatcatt acattatcct tacgcaattc
     3121 tttgatagca ttatggatag ttgaaggtaa gtcctggata tgtgcagctt ctctttcttc
     3181 ctcatccatt acataaatgt tacggtcaac agctgctggc ggtgtgatct gattacggat
     3241 accgtctagt cctgcttgca acaatactgc cattgccaag tatgggtttg cagatggatc
     3301 aactgaccgc aattccaaac gtgtggataa accgcgagat tctggtacac gtactaatgg
     3361 cgaacgatta cggccactcc atgcgacata aacaggcgct tcataacctg gaacgagacg
     3421 tttataagaa ttgactgttg gattgcaaac ggctgtgtag gcacgagcat gtttcagcaa
     3481 accacctaag aaatgataag ctgtttcact tagttccaat tcaccgtctt tatcatagaa
     3541 aacatttcct tcatcattga acaaagacat attgcaatgc ataccagaac cattgatccc
     3601 aaataacggt ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa tcgttttgac
     3661 aacgagcttg aaggtttgga tattatcaca agcatctacc acatctgcat atttaaagtc
     3721 aatttcatgc tgtcctggcg ctacttcatg atgtgaagct tccacttcaa agcctagact
     3781 ttctaattcc aacacgatgt cacggcggca attttcacct aaatctgttg gtgcaaaatc
     3841 aaaataacca cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt ctaatttaaa
     3901 taagaagaat tctggttcag gtcctaaatt gaaagaggta aaaccaaggt cgcgcatatc
     3961 tttcaatgct cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc catccggatt
     4021 atagatatca caaattaaac gagcaacttt tccatgagca ctttcccacg ggaagatcat
     4081 ccaagtggag agatctggat acaaatacat atcgctttct tcaattcgaa caaatccttc
     4141 aatcgaagaa ccgtcaaaca tcattttatt ttctaatact ttgtcgatct ggctcactgg
     4201 aacttctaca tttttgatcg tacctaagat gtctgtgaac atcaatcgta aaaatcgtac
     4261 gttttcttcc tcaatgattc gtttgatttc ttcacctgtt atctgtttct ttaccatggt
     4321 atccctcttc cttttttaat ttattcagtc aaagaattgt ctttgcaatc cttgcgactg
     4381 ataatgacct gttttgctta acccaccttg cgccaaaatc tcatcacgaa aaatttgtcg
     4441 aacgtcatga tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg cctgtgccat
     4501 ttcgtataca cgtttgatcc cagccatatt caatccctct gaaagatagt ctttaatctc
     4561 aagcaacata tcaatatcat tcagcgaata catacgtcgg tttccttcgc ttcgttccgg
     4621 atgaactaaa tcttgttctt cataatagcg aatttgccga gcagacaaat cagtcagctt
     4681 cataactgta ccaattggaa atacggacat agatcggcgc aattcctttt ctcccatcag
     4741 acgtcccctt tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa tattgtcaac
     4801 tgcatatgtt aggttttcta acatgagatc tctatttttt ataaaataac aaaaaaataa
     4861 gaacacaaaa aaggtatcta gttgattttc gtaccagata ccttttattt tttattatac
     4921 tgtactatcc tttattagtg tttttatt
//
LOCUS       DACDHJ010000167         4899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDHJ010000167 DACDHJ010000000
VERSION     DACDHJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.170"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..673
                     /locus_tag="IV223_002157"
     CDS             <1..673
                     /locus_tag="IV223_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8712416.1"
                     /translation="YELGLDFLLVNIPTLFSSYLGDSGKTITKLFKSLSSKKAIIVFD
                     EFDSIAVGRYSMNDVGEMRRIVNSVLTGLDNWEGEGLIIATTNDKENLDNAVWRRFDE
                     KIKVGLPDKENRLKLWDMYSKSQLTRDELHILAEVSNGFSPAEIEIYSQQGLRLKVIK
                     GKNPFLTIMNQLEVPKLSLEKKVFLVKYLKSNYPNLTTRDIGELMNISKSSVQRYLKG
                     VSEVE"
     gene            660..2900
                     /locus_tag="IV223_002158"
     CDS             660..2900
                     /locus_tag="IV223_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAP8712417.1"
                     /translation="MKLNEKKHLLVINGKALAETGVIPPPTGDSRIEERKYKRNLAQA
                     KEYLLPQINQLRLDVEELPQENILKNIYFRISYPNGFLAKSYQVSSIYRDSGLTLIGS
                     SSWKDDEGKEGKSDYLFGTKDNIEALSLAISDSSVQIQQKEIRRMEEISLLKPLIFDD
                     SESKSYELVFHSVENIEELLSKFQAITGLNSNEYSFIHTVNNVVFIVCKLTNEKLINL
                     RNFNPLRAAYSSENREFSADNFFPEQILNKYTDISLDSESVKQLPWIGLIDGGVSKSN
                     NFFLPVEQIHESTAPASSRFIEHGSSVASVLLYGELDGQNGGGELVPSFRVQSIRALP
                     SEEDMEFNLLTLDKMIMEIVPKYSNIRVWNLSIGPVGPIQDEVVSSLTRILDQIAYEN
                     DIIFVIAAGNTGEHTGIAKRIQIPGDAVNNLTVSAYYKHHENKITSPYSSIGLGREGA
                     KLKPDLIDHGGLLPIDPIYTISSSNYFLNKVQGTSFSAPNVARKLALILNEYPDFNVW
                     EARALLEHSLALRISSAKDIQFEGKGEFDKGSVQLIASEEDEIRIMYSGIISAKGYVI
                     LPIPIPEETIAKTAEFTWTLVTKTKINPDNTDKYTEYGIEDDFYPNADKYPFRKGDKT
                     IYVDLSSEEGKASAEVLLEEGFKKSSYPQKQPSKYLDETERRNKFLKWDTSKTQRIRK
                     RVHGLKRPFIRLHGLSRSENRDRIEYVLVVTVRFVKDTSIYDNVMSKYPILQSIQVRS
                     EGRQQV"
     gene            complement(3301..4443)
                     /locus_tag="IV223_002159"
     CDS             complement(3301..4443)
                     /locus_tag="IV223_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8712418.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(4482..>4899)
                     /locus_tag="IV223_002160"
     CDS             complement(4482..>4899)
                     /locus_tag="IV223_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8712419.1"
                     /translation="KNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKV
                     KNEKISLFNILKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYP
                     EEAVREITDNINKLEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT         1575 a          749 c          891 g         1684 t
ORIGIN      
        1 atatgaatta ggactagatt ttcttttagt taatatacct actctttttt caagttattt
       61 gggagattca ggaaaaacta ttaccaagct atttaaatct ttgagttcaa aaaaagcgat
      121 aatagtcttt gatgaatttg atagtattgc agtgggtaga tattcaatga atgatgtagg
      181 ggaaatgaga cgaattgtca attccgtatt aactggtttg gataattggg aaggtgaagg
      241 cttaattatt gctacaacaa acgacaaaga aaatcttgat aatgcggtat ggcggagatt
      301 tgatgagaaa attaaggttg gtttaccaga taaagagaat cgtctaaagc tttgggatat
      361 gtattcaaaa agtcaactaa caagagatga attacatatt ttagcagaag taagtaatgg
      421 cttttctcct gcagaaatag aaatatattc tcaacaaggt ttgcgtctca aggttattaa
      481 gggcaaaaat ccatttttaa caattatgaa ccagttagaa gtcccaaaac taagtctcga
      541 aaaaaaagtt tttttggtta aatatttgaa atccaattat cccaacctga ctacaaggga
      601 tattggtgaa ttaatgaata tttcaaaaag ttctgtgcaa agatatttga agggggtatc
      661 tgaagttgaa tgaaaagaaa cacttattag ttattaatgg gaaagctctt gcagaaacag
      721 gagtgatacc acccccaaca ggagactcaa ggattgagga acggaagtac aaaagaaatt
      781 tagctcaagc taaggaatat ttgttacctc aaatcaacca attaagatta gatgtagaag
      841 aattgccaca agaaaacatt ttgaagaaca tttattttag aattagttac ccaaatggtt
      901 ttcttgcaaa atcttatcag gtatcttcaa tatatcgaga ctcaggactt acattgatag
      961 gttcatcttc ttggaaagat gatgaaggaa aagaaggaaa atcagactat ctttttggca
     1021 ctaaagataa tattgaagct ctttctttgg cgataagtga ttcttctgta caaatacagc
     1081 aaaaagaaat tcgtagaatg gaagaaattt cgttattaaa accgctcatt tttgacgata
     1141 gtgaaagtaa atcatatgaa ttagtttttc attcagtaga gaatattgaa gagctacttt
     1201 caaaatttca ggcaataaca ggattaaata gcaatgaata tagttttatt cacactgtta
     1261 acaatgtggt atttatagta tgcaaattga caaatgaaaa attaattaat ttgaggaatt
     1321 ttaatccatt gagagctgct tattcttcag aaaacagaga attttcagct gataattttt
     1381 ttcctgaaca gatacttaat aaatatactg atataagttt ggatagtgaa tctgtaaagc
     1441 aattaccttg gattggttta attgatggag gagtttcaaa atcgaataac ttctttttgc
     1501 ccgtagagca aattcatgaa tcaacagctc ctgctagttc gcggtttata gaacatggat
     1561 catcggtagc tagtgtgctt ctttatggag agttagacgg tcagaatggc ggaggagagc
     1621 tagtgccctc tttcagagtt cagagcattc gtgcattacc gtcagaggaa gatatggagt
     1681 ttaacctttt aactttggat aagatgataa tggaaatagt tcctaagtat tctaatattc
     1741 gggtttggaa tctttcgatt ggaccagttg gacctataca agatgaggtt gtttcttcat
     1801 taactaggat tttggatcaa atagcttatg aaaatgatat tatttttgtt attgcggcag
     1861 gcaatacggg tgaacatacc ggtattgcta aaagaattca aatacctggt gatgctgtta
     1921 ataatcttac agtttctgct tactataaac atcatgaaaa caaaattacg agtccgtaca
     1981 gttctattgg attaggtagg gaaggtgcta aactcaaacc tgacttaatt gatcatgggg
     2041 ggttacttcc tattgatcca atatacacaa tttcttcgtc caattacttt ttaaataaag
     2101 tacaaggcac ctctttttct gctccgaatg tagcaagaaa gttagcttta attttgaatg
     2161 aatatccgga ttttaatgtt tgggaagctc gtgccctgtt agaacactct ctggctttac
     2221 ggatatcttc tgctaaagat attcaatttg aagggaaggg cgagtttgac aaaggatcgg
     2281 ttcaattgat tgcttctgaa gaggatgaga ttagaattat gtattctgga attatttctg
     2341 caaaaggata tgtaatatta cctattccga taccagaaga gacaattgct aagacagcgg
     2401 agttcacttg gactcttgtg actaaaacaa aaatcaaccc tgataatact gataagtata
     2461 cagaatatgg aatagaagat gatttctacc caaacgctga caagtaccct tttcgtaaag
     2521 gagataagac aatctatgtt gatttatcat ctgaggaagg caaagcttca gcagaagtat
     2581 tattagagga aggctttaaa aaaagttctt atccccaaaa gcagccttca aaatatttag
     2641 atgaaactga aagaagaaat aaatttttaa agtgggatac atcaaaaact caaagaatac
     2701 gaaaacgagt acatggattg aaaaggccct ttattcgtct acatggtcta tctagatcag
     2761 aaaatcgaga tagaattgaa tatgtattag tagtgactgt tagatttgta aaagatacaa
     2821 gtatttacga caatgtgatg tccaagtatc ctatattaca atcaattcag gtgagatccg
     2881 agggaagaca acaagtgtaa taattaatat gttgcttttc gaaagcttga tacagtatgt
     2941 taaaataagt ttggttacca accgaaacta ttttacaaaa ctggattaca aatagtaaat
     3001 caatctctgt attgatgtga aataattgat tctccaaata acttctctac ctgctgtttt
     3061 ggctacgata ctggctacgt tctcgatgat attacctgat aatatacact gtcagtctat
     3121 ggaaatttgt taaatgaaat ctttatttaa agcctgttcc gttccattgc ttcccaaaag
     3181 agtcattgga aggcagtgtt gggtaaacct gttataggag ctagaaccct tgatatataa
     3241 gggttctagc ttttttgttt tgcctgtata ctagaaatga ccagattttg accagctctt
     3301 ttactgatga gccatcatat cagcaaagtc ttgacctgtt ttcttaggtg cattatttgt
     3361 aatatgtgca tagatgtcta atgtcacttt actattttca tgccctaatc gttgttgaac
     3421 taccttgaca gattctcctg cttctaataa taaactagca tgtgtatgtc taaacccatg
     3481 aggtgtaata cgaggcaagt tatatttttt caaaatatat cttaaccaat cattaacagt
     3541 gtttggaatg tatagagtgt ttttaactgt tgtaaaaaca tgttgctctt tagagctagt
     3601 attgtatcca agttttaaat actcttcttt ttgctgtaat ttccagttgt tgaggataga
     3661 aagagtctca gtatctaaag agataactcg tctagaagct cttgttttcg gagattgaat
     3721 tataacttta ccaaattcat ctacagcaag agttttattg acatgtagtt ctttattaaa
     3781 cgtgtctatg tctttccact gtaaagcgag tacttcactc tttctcatac ctgtgtatgc
     3841 caataatctg aagaaggcgt attgtttcat gttaccaaaa tctttaaaag catccagtaa
     3901 attattaagc tcctcttttg agtaaaattt acgatttttc tcatactctt tttttcgtgg
     3961 aagaagtgtt ttttccatag gattattgtc aatatattgc attgcaaccc cgtagcgtaa
     4021 aatttgtgca gtctgttttc tcatataatc gtaactttca tatttactgt gccattcatc
     4081 taccactttt tgacaatatg ggacagagat attcgatagt ttaagcttac caaatgctgg
     4141 aagtatttga ttatttgcgt atcttactga gattgcgatt gttgaagctt taacactggt
     4201 acgctgatga gttatccatt cattatacag gtcttcaaat gtcatatcag gtgctttctt
     4261 tttaacaccg tataatattt gatgtctgta ctcgtcataa tctttttgag cttcttgttt
     4321 gctactaaaa cctcttctcg tagtatttat tttttcacca gttcgtggat cggtggcaat
     4381 gtgtccttta tacatccacg ccacttgacc gtttttcttt ttgtactttg atatttttgc
     4441 cattaaatca tctcctctta gtttacaagg agtacttacc actagatggt atcacctccc
     4501 ttcatttttt tcttagtgta ttttttccca attgatgaaa tcgtttgttc tagcttgttt
     4561 atattatcag taatttctct cacagcttct tcgggataag acatgtcttt caattgaata
     4621 ggtagccatt catctaccat tctttctagt agttcttcta tactgctagt gaggaaaaga
     4681 atgttattgt caaattcttc tacaatagca ttgatgaata attcttctat ttttaagata
     4741 ttgaaaagac taattttttc atttttaact ttattacaag tcttgttagc aataaattct
     4801 tgcatctctg gttctaattt ggaatatcta tccagccatg tatctaaatt agagtgtact
     4861 ggtgggaact gttgacgaag atgctggaac aggttttta
//
LOCUS       DACDHJ010000168         4767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDHJ010000168 DACDHJ010000000
VERSION     DACDHJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.171"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..794
                     /gene="purR"
                     /locus_tag="IV223_002161"
     CDS             9..794
                     /gene="purR"
                     /locus_tag="IV223_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8712420.1"
                     /translation="MTQYLLNHPHELISLTSFAERYESAKSSISEDLGIIKKTFKERG
                     YGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGYVYLSDLLGEPE
                     LLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVRRDSKITEGSTV
                     SVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSLIEEFESELVGI
                     TVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1297..2670
                     /gene="glmU"
                     /locus_tag="IV223_002162"
     CDS             1297..2670
                     /gene="glmU"
                     /locus_tag="IV223_002162"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8712421.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2764..3735
                     /locus_tag="IV223_002163"
     CDS             2764..3735
                     /locus_tag="IV223_002163"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8712422.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3741..3843
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3909..4478
                     /gene="thiT"
                     /locus_tag="IV223_002164"
     CDS             3909..4478
                     /gene="thiT"
                     /locus_tag="IV223_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8712423.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4559..>4767)
                     /locus_tag="IV223_002165"
     CDS             complement(4559..>4767)
                     /locus_tag="IV223_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712424.1"
                     /translation="EGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIAT
                     LIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1550 a          847 c         1051 g         1319 t
ORIGIN      
        1 tagttgacat gacacagtat ttattgaatc acccgcatga attgatttcc ttaacaagct
       61 ttgccgaacg ttatgaatct gctaaatctt ctatcagtga agatttggga atcatcaaga
      121 aaacatttaa agaacgaggg tatggaacct tggaaacaat cccgggtgct gcaggtggtg
      181 tattgttcat ccctgaaatt tcatatgaag aggcgaaaaa atatattgaa tctctagcag
      241 aacgtctatc agaacaagac cgtctgcttc caggaggata tgtttatctc tctgatttgt
      301 taggagaacc tgaactgctg cgtcaagttg gaagaatcat tgcttctaaa taccttggtg
      361 aaaaaatcga tgctgtaatg actgtagcca caaaaggcgt acctattgcc caagctgtgt
      421 cctattatct gaatgcgcca ttcgttattg tacgccggga ctcaaaaata acagaaggat
      481 caacagtaag tgtcaactat gtgtctggat cttctgaaag aattgaaaag atggaactgt
      541 ctaagagaag tttgaaacgt ggttcacgtg tccttgtagt ggatgatttc atgaaaggcg
      601 gcggaacagt caacggaatg aagagcttga ttgaagaatt cgaatcagaa cttgttggca
      661 tcactgtatt tgccgaatca aaattcaacg gtcatcgcgc catcgatgat tatacgtcac
      721 tgctttatgt caaagatgta gatactcaaa cgaaaaatat tacggttgtg ccaggaaatt
      781 atttttcaga ataagaggtt gtgaaaaagc cgcttagttt cgagtagtaa gaaaaatttt
      841 gtgaaaatgg ctttccacat tttttcataa atttggctta ataccgagaa actggctttt
      901 gaacaccgtt tatctagagg ttgtgaaatc agcgtcctgt cctgagtatt agctcaataa
      961 acggaaaaat tgctcctcat attttgccgt ttattgagct aataccgaag gtcagctgat
     1021 tgaaaaacgt ttaatgaaga ggttgtaagc ctaccgttta actctaagtc acaagaaacg
     1081 atttcaaaaa acagcttttc atgttttatg agtagtgtga cttgtgaccg aggagttggc
     1141 aggagagcac cggttaccaa atagataggc ccgttatggt ctatctattt ttttatccag
     1201 aaaacgctgg gataaaagaa aatcacttgt tttgttgaaa aaatgggtat actgggataa
     1261 actgaaatca gcaaactata tttaggagtg acgcagttgg acgcacgtta tgcaattatt
     1321 ttggcggcag ggaaaggtac ccgcatgaag tcaaaattat ataaagtttt acatcccgtt
     1381 tctggtaaac ctatggtaga acatatcatt aatcgggtca gtgaaacgaa accagatgaa
     1441 gtgattacga tcgttggaca cggagcagaa caagttaaag cacaactagg agaacgcagt
     1501 aaatatgcgt tacaagcaga acaattggga acaggacatg ctgttttgca agctgcttct
     1561 ttcttggaag gaaaaaaagg aacaacgtta gttatcagtg gcgatacacc attattgact
     1621 actgaaacgc tgaacaatct ttttgaatat caccaaggaa aaaatgcaag tgcaacgatc
     1681 ttgacggcac aagcagaaaa cccaacagga tatggacgaa tcatccgtga ccatattggg
     1741 atcgtagaaa aaatcgtgga acaaaaagat gcaacaccag aagaagctct tgtacaagaa
     1801 atcaatacag gaacttactg ttttgacaac gaagctttgt tcgatgcgtt aagcaaagtt
     1861 ggaacaaaca atgctcaagg tgaatactat ctaacagata tcatcgaaat cttaaaagaa
     1921 gaaggccata cagttgctgc ttatcaaacg gatgattttg aagaatcaat gggagtcaat
     1981 gatcggatcg cgttagctaa agcaaatgag atcatgagaa agcgaattaa ccagatgcat
     2041 atggtcaatg gcgtaagctt tgtcgattca gcaacgacat acatcgatgc aggcgtagaa
     2101 atcggtccag acacattgat cgaagcaggt gtacaaatcc aaggaaatac agtcattggt
     2161 tctgattgtg tcatcgggtc acattcaaaa atcgtagaca gtaggattga ggatcacgtc
     2221 gtcattgaaa actcagtcat tgaaagcagc catgtcaaga aacatgcaga tgtgggacct
     2281 tatgcacatt tacgaccaaa agctgaaatt ggcgaaaacg tccatatcgg aaactttgtg
     2341 gaagtgaaaa atgctcaaat cggcaaagga actaaagtcg gtcacttgac ttatgttggt
     2401 gatgctacgc tgggagaaga aatcaatgtc ggatgtggtg ttgtcttcgt caactatgat
     2461 ggaaagaaca aacatcatac gactgtcggc gaccacagct ttattggttc tagcacgaat
     2521 atcatcggac ctgtcgaggt agctaaaaac agttcgattg ctgccgggtc aacaattaca
     2581 gacaatattc cagaatatgc gctggcaatt gcgagagcaa gacaagtcaa taaagaagga
     2641 tatgcaaaaa aactccctta tctaaactaa gacttttgca aaatcacttg atttatttaa
     2701 aaaatatgac tactatttat taggtagaat tctagagttg tgaaaaagaa agtggaggtc
     2761 cccatgtcaa aacattactt tgacccaaga ttaaaaattt ttgcgttaaa ttccaatcgc
     2821 ccattagcag agaaaattgc agcagcagtc ggcgtcgagt taggaaaatc atctgttact
     2881 caatttagtg atggagaaat tcaagtaaac attgaagaaa gtattcgtgg atcgcatgtc
     2941 tatgttattc aatcaacgag cagcccagtc aatgataact tgatggagtt gctgatcatg
     3001 attgacgcac tgaaacgagc aagtgcaaaa acaatcaacg tagtcatgcc ttattatggc
     3061 tatgcgcgtc aagaccgtaa agcacgtgca cgggaaccaa tcacagcgaa attagtagca
     3121 aacatgattc aaaaagctgg tgcaacaaga atgctgactt tggaccttca tgcagtccaa
     3181 atccaaggtt tctttgatat tccagtcgat catttgatgg gtgcgccact tattgccaat
     3241 tatttcttag aaaaaggaat caaaggcgat gatgttgtcg ttgtttcacc agaccatggt
     3301 ggtgtcacac gggcacgtaa attagcggaa tttttgaaat ctccgattgc gatcatagac
     3361 aaacgccgtc caaaagcaaa tgttgcagaa gtaatgaata tcattggaca agtcaaaggc
     3421 aaaacttgtg tactgatcga tgatatgatc gatacagcag gtacgatcac attggctgcc
     3481 aatgctttga aagaagctgg tgcaactagt gtgtatgctt catgtacaca tccagtatta
     3541 tccggccctg ctttacaaag aatagaagat tcagctatcg agcgtctagt tgttacagac
     3601 tcgatctatc ttccagaaga tcgtaaaatc gacaaaatcg atgaagtaag tgttggcgga
     3661 ctaatgggag atgcaatcaa acgtatccat gaaaacaaac cagttagtcc attgtttgaa
     3721 acaaaaaata aataataaat aaactcacaa aggggagtcc aattgggctg agattgaatc
     3781 tatttctaaa cccttcgtac ctgtatcggt tatgcgagcg taggaattgt gaataaacag
     3841 ctgtttgact attttcgaaa gctgcttcct cctttgtgac ttttttattc aaaggaggaa
     3901 gtttttttat gggaaaaaat agagtttggg tagaaggaac gattgttgca gcgttagcga
     3961 tggtgttgtc gttgattccg atccagatcg gcagcagttt ttctatttca ttagggcaga
     4021 ttccattgac tctttttgcg ttaagaagag ggacaaaggc agggatgctg gcaggtttca
     4081 tttggggcat cctacatttt ccgttgggac aagtttatta tttaagtgtc gttcaagtgt
     4141 tgattgagta cccgattgcc tatacttttg caggcgcagc agggctcatg gcttcaaatg
     4201 tccaaaaagg ctatgtagac gataatacga aacaaattag aaaaagcatc gtgtttggtg
     4261 cattgattgg cgcactttcc agatattttt ggcattttat tgcaggagtg gttttctggg
     4321 gagcgtatgc actatggggg atgaatcctt ggctcttttc tttcgtaatg aatggtgcca
     4381 gcgggttgag tacagctatt gtaacgatca tcgttttatt agggatccaa caaacgactc
     4441 cttcgatttt tgtgccagat aaacgacagg agctgtaaat aaaaaagaag caagaaactt
     4501 gagacagaat catgtcctag tttcttgttt cctttttatt ttttgataag aaatagatct
     4561 acttcaatgg cagataagaa agatcggcat cttctaccaa ttgccagtgt cgatcggtga
     4621 cagagtagat taaagtagcg atgatttcct cttctcgata ccgggtgcct tttaatttcc
     4681 aaatataatc ttcaatcaca tagattggga tacgatattt accatcgatc aaaccaactt
     4741 gatcaaaaga aaaaatgatc ccttcct
//
LOCUS       DACDHJ010000169         4733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDHJ010000169 DACDHJ010000000
VERSION     DACDHJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.172"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            97..411
                     /locus_tag="IV223_002166"
     CDS             97..411
                     /locus_tag="IV223_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8712425.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            413..2068
                     /locus_tag="IV223_002167"
     CDS             413..2068
                     /locus_tag="IV223_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8712426.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2151..4286
                     /locus_tag="IV223_002168"
     CDS             2151..4286
                     /locus_tag="IV223_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8712427.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            4461..>4733
                     /locus_tag="IV223_002169"
     CDS             4461..>4733
                     /locus_tag="IV223_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712428.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITS"
BASE COUNT         1644 a          796 c          997 g         1296 t
ORIGIN      
        1 aaaaaaatga agagtcattc tggttgacat attaataaag ctcctgttta atatacccca
       61 tgaggtattt ataaaaatgt aaatgaggtg cagaaaatga atttgctatt tcaatcaata
      121 aaatcgataa cagtcccaga attaaaggaa aagcttttgg aaaatccagc gttacttgat
      181 gttagaacac ctgcagaata ccggggcggg catataaaag gagctaaaaa tgttccatta
      241 caaagtataa acagatatga tggagacaaa gaaaagaccg tctatgtgat ttgtcaatca
      301 gggatgagga gtaaacaagc agctaaagaa ttaaagaaat ctggttatga tgtagtgaat
      361 gtacgtggtg gcatgaatca atggtttgat cgaacagtag gagggaaata agatgaaaat
      421 cgttattgtc ggaggtgtag caggtggaat gtctgctgct acacggcttc gccgattaat
      481 ggaagatgca gaaatcgttg tttttgaaaa aggaccgtat gtttcttttg caaattgcgg
      541 cttgccttat tatctttcgg gagaaatcag tgaacgggaa aatcttcttg tccaaacgcc
      601 agaatcatta tccgctcgtt tttgtttaga tgtgcgtcca aatcatgaag tgacagccat
      661 ctttcccgaa aacaaaacgg tagaagtcgt acatgagggt caaaaacaca ttgaacagta
      721 cgatgcattg gttttatctc ctggtgcaaa accagttgtt ccatcgattc cagggataac
      781 agaagccgac aatgtttttt ctattagaaa tgtaccagat atcgataaag tgatgcatgc
      841 attagaaaaa cagccaaagc gtgccgtgat cgttggtgca ggattcatcg gattggaaat
      901 ggcagaaaac ctaaaaagaa gaggtttaga agtcatggtg atcgaacaag caccacatat
      961 tcttccaaca ctggatgaag aaatggcagc ttttatagaa aaagaattgt ctcatcaagg
     1021 agtagaagtg attacttctc atgctgtcgc tggatttgaa gaccacggga aacgattgcg
     1081 actggatgat gggcgtacca tccctgctga tttagttatt ttatccattg gtgttcgtcc
     1141 tgataaccag ctagcagtga ccgctggaat cgaattaggc atacgcgggg gtatcctagt
     1201 agacgaacgt tatcaaacga atattcctga tatttatgcg gtgggggatg ctatcgttgt
     1261 aaaacagcaa atcactggaa aagatgcact tatttctctt gcttcaccag ccaatcgtca
     1321 aggtagacaa gttgcggaca cgatttccgg aatttctcga agaaatcaag gcggtattgg
     1381 aacagcaatt atacgaacgt ttggaatgac tgccgcatcc accggtttaa gtgaaagaac
     1441 agccaaagaa aacgaactgt cttttgaagt cattcatgta tcaggaaaag atcatgcaag
     1501 ctattatcca gaagcaacag atattttact gaagttgatc ttccatccag agactggcga
     1561 gatttatggt gcacaaggtg ttggggcaaa aggtgtggat aaacggatcg atattttagc
     1621 aacagcaatc aaagggcatt tgacgatctt cgatttaccg gaattagaat tgacgtatgc
     1681 accgccattt ggctcagcca aagatccagt aaacatgcta ggatatgcag caatgaacat
     1741 tgcagaaggg cttagtgaaa ccgtacaatg gcatgaattg ccgacagaac tagcaaaagg
     1801 aaaaatttta ttagatgtgc gaacagcaga agaattggaa aagggcaaat tcaaggaagc
     1861 caaacatatc cctttgaatg aacttcggga ccgattaaat gaattagaca gccaacaaga
     1921 atatatcgtc agctgtcata gcggattacg tagctatcta gcggaaagaa tcttgaagca
     1981 gtctggctac cacgtaaaaa accttgatgg tgcattttct ttatatcaaa ctgtccgacc
     2041 tgaagaactg atatatccta acaaatgata ctcgttaaaa agcttgcatt cgactcttaa
     2101 aagaggtaaa ctaatcggta ccaccacttt aaaaaaagag gagaatgtgt atggtcaaca
     2161 cacaaaaaca gttagaagaa gcatatctga agaacgtgta tcaaaagcta attgataaaa
     2221 aaaacgaact gcgggaatcg atggatacgg caaaaataga aggaaaccag tcgttgaaag
     2281 aaatggctgg cgagcttcga ttgaattttg acagttactt agataacctg gatacttttt
     2341 cgatgattga aatgaaaaat agagaaatcg atcagatgaa tatcaaacag aaaaacgcag
     2401 aaaatcagct agcaaagatc gaacgtttat tgaaaagtcc ctactttgga aaagtcgttg
     2461 tagactttct ggataatgag ccaaaagaat ccttttatat cggtacagca aatttcacag
     2521 atgatgcaga tgaaaatctt gtttatgatt ggcgttcacc aattgccgaa cttttttaca
     2581 ataacatgat tggtccatct tcttatacgg ttcgtcaaaa taaaatcgat acggttatta
     2641 aagaaagacg acaatttctt ttggaaagag atcgtttggt caagtttttt gatacgtctg
     2701 tagctattca agatgacgta ttattagaag tcttaggcca agatgagacc aatgaaatga
     2761 aggctatcac tgctacgatc caatcggaac aaaacacaat cattcgggat gtgaaaagtc
     2821 aaaatatctt ggtaaatggc gtagcaggaa gtggtaaaac ctctgctatc atgcagcgta
     2881 ttgcgtatct gctctattta tatcgagata cgatgacatc cgatgacttg ctgattctat
     2941 cacctaatca tcggtttatc gactatattt cgaatgtcct accatcatta ggtgaaagaa
     3001 atccactgaa tctaaccatc atacagctgg tttcacagct ttcagcagag gaaatagaag
     3061 gtgaagaggc ttatttcaaa cgtatcacgg gagaaaatgt atcggagcag acagaacgtt
     3121 tgcgtagcaa aaagtttatc gataatctaa aacaatcaga tcctttgttt ttagatcatc
     3181 cgaattttat ccgtggactt actaaaaatg gaaaaacagt cctttctaaa aaaacgatcg
     3241 aaaaaatata tgagaaagtt cctgctcatc ctaaattgat cgataggctt caagcaacga
     3301 aaaaggcact gatgagcgaa tggaagaatc atcttttgaa acaagcaaaa agtcctgctg
     3361 tgcagaatca ggtactctcg ttgacagaag ataggcagtt ggaattattc ggtaaattga
     3421 tttctgatga ttcggaacaa agtattgtag cttatgcaag gaaactttta caaaaaaaat
     3481 atcgaaaaat aaccagacaa atcgaagaaa tggcatgggt ggatgagcat cagttgtttg
     3541 aaagaattta tgaaaaacga tatggctcag cttatgcttg gcaacccaca cgtactgttg
     3601 atgaagcagt cattatatta gctattcgtc atttacttgt tgaaaaggta aatgtaccag
     3661 catttcgcta tctattgatt gatgaagtac aagattatac cctggcacaa ttaggtctat
     3721 tgatcgaatt atttccaaaa actcacttta ctttagtagg tgacgagaat caggcaatct
     3781 ttaattcttc cactactttt gctgatatta tgcgttgttt cgacgactac catctgccga
     3841 tccatcggta tgatcttaga aatagttacc gttctagcgg agcaatcaca gagcttttca
     3901 aaacctatgc tgtggatcaa gagaagatcg acatcgtatc gatccgaccg caaggaaaag
     3961 aacccgaata ccgctctttt cgatcctctg aagaattgtt gaaaatacta gctgagattt
     4021 tggtttctct aaaaggagaa aaagcggcag ttatcaccca aacagaagag gaaacccaaa
     4081 cgctacaaga atcgattaaa aaaaattcat atggattaga aaactgccaa atcattcctt
     4141 tgagtctagc aaaaggactg gaatttgatc atgtaatctt atatccattt gaaaacgatg
     4201 gagatgagca aagaagacgc agacaaatgt acacggcgat atctagagga atgaaaagca
     4261 ttgtcgtatt ggaaagagca acataattaa gagataaaaa ataaaaagaa caactgataa
     4321 aaagaacaaa atatttttca tttgctgatg agaaatgttt tgttcttttt cttaggaaaa
     4381 atttaggaac actttatgtg aaaagaaatg aaatgtatgg taaactccta cgtcgtaaca
     4441 aaaaaacgtg aggtgaggat atgaaaaaag taacaagaga agaagttgca tcaattttgg
     4501 tgaaagataa gtatttttca aaaggtaatt actggttaaa gatttggcaa acgttagtgg
     4561 ctcttttagg atggatgatc gttgttcttc cttttatatg ggtctttttc ccattaacga
     4621 gaccagaaag agccaaggat ttttccttgt acgctttttt atcagaaaag aaaatgctgt
     4681 atttcttaat cggctttttt gtattgattt ttttcagttt cctgattacc tca
//
LOCUS       DACDHJ010000170         4729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDHJ010000170 DACDHJ010000000
VERSION     DACDHJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.173"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..1073)
                     /locus_tag="IV223_002170"
     CDS             complement(69..1073)
                     /locus_tag="IV223_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8712429.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1144..1440)
                     /locus_tag="IV223_002171"
     CDS             complement(1144..1440)
                     /locus_tag="IV223_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712430.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1433..1726)
                     /locus_tag="IV223_002172"
     CDS             complement(1433..1726)
                     /locus_tag="IV223_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712431.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1872..2423)
                     /locus_tag="IV223_002173"
     CDS             complement(1872..2423)
                     /locus_tag="IV223_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8712432.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2597..2896)
                     /locus_tag="IV223_002174"
     CDS             complement(2597..2896)
                     /locus_tag="IV223_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8712433.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2923..3231)
                     /locus_tag="IV223_002175"
     CDS             complement(2923..3231)
                     /locus_tag="IV223_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8712434.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3348..4307)
                     /locus_tag="IV223_002176"
     CDS             complement(3348..4307)
                     /locus_tag="IV223_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8712435.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4451..>4729
                     /locus_tag="IV223_002177"
     CDS             4451..>4729
                     /locus_tag="IV223_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8712436.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLST"
BASE COUNT         1376 a          894 c          728 g         1731 t
ORIGIN      
        1 aactgattct aagaaaatta aataattttt ctcaaaatca gtttttcttt gataaataat
       61 attttcaatt atttaatcat ttgaaaattg ttagtcattc caatacgttg agaaattctt
      121 ccatctgtat aaacacggaa ttgttttcca ccagccattg ttgtatttcc attatcatcc
      181 gttacaatat taattgaata gtagcttcca ttttcatctt gcattagatc tttcatatat
      241 tcatttgtgc taattaaatt agtatttgat gcatttttat caggagaatc atacaaccat
      301 aaagctttat tatttagtac agtttcttta gcagcttccc aacttgaaat tgctgagcta
      361 cttgtagatt gtgtcgttgt agttgttgct tgttgagtag attcttctac tactggagtt
      421 tgaggagttt gtccattcac aatatcaatg aaaccttgtt ttaactgttg gttctgagtt
      481 tcattgaagt acatgtagtt tttatcattg ccttgatttt gttgcgttaa ataaacttta
      541 ggttgtccat tttgaaagcc aaagaaatat acatgctgcc ctaaatattc tccagtttct
      601 acatcagaat aaactgctac taaattgaaa tctgcatgac cagttccatc ttctgaccat
      661 tgtacggtaa ctggtgcttc attgattgcc attttccaat ttccatcaat aactgcactt
      721 ggtaaaggcg taccatacag acttacattg ttctgaggat tgtagctttt atattcttga
      781 cctaatgtct ttccccattg tgggacaaaa attgctagtt gagtagattt ctcactatcc
      841 cacaaagtat ttttaacctc ttcagaagaa gattcaatga ttgaactttc acttgtactt
      901 gcttccacag attgtttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagcctcaa ttgttgtatc agtttctaca gtctctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaatccc cattccaatt agtaataaca accatttttt cattttattt
     1081 ccccctttca atttctccct cgcgtactta ttttatcagt caattaatct caatggtgtt
     1141 catttagtta atcctttccc aagaatgtcg atagccagca tgacaacgat ctcttccttg
     1201 agggctttca gatggattaa aactagtaga aatttccctt ccacatcctc tacagcgata
     1261 agttctcatt atttttgggc tattagtatt atttttctta gttagagcta tgagtccaac
     1321 tatgattacc ccaactgtct taattacttt tcctttatct atattcggat gcgcaagcat
     1381 atatttatat aaccaatatc caccgtaaat taaaccacct aatacaatca gcttagccat
     1441 gtactcgtct cctttctttt tgtttaatta ctttttgaat ccatcttgca gagaaaccat
     1501 aaacttctgt caattgacgg atatctacag ttttttcctg ttctatcatg ttttcgattc
     1561 tttttctaac taactcacga tcgtacattt tcattggaaa agaaatctgt tgccctcgaa
     1621 aagcaagata aaattgatag agcaattctg aatccagttc catttcctca aaaaagtcat
     1681 tcatactttt ataagagtta ctcaaacaag tttcatcaat ctccatctac acaccccctg
     1741 ttatttttta cataatgaaa atagcatatt actgggtaca ttgataattc atataacatg
     1801 tatcatcaca tgtattttta aagtcaaaaa gcatgtaaaa ctacttataa gtaacataag
     1861 aaaataaaat gttatttttc ttgaaataat aattgactat ctgccaattg ccattcatta
     1921 cgaacatgaa tacttagtgg gtgggataac ttaccttctg catttatcaa tactttggtc
     1981 atcttttttt gtttcgcact caattgatcg taaatggtct tcgcttgttc ataagccact
     2041 tgactcgtcg ctgtaatcga accacaagca aaaataatgg tgttgatttt cttttctgtg
     2101 aaaacagttg ttaatatttc caaagattgt tcatcatttc cacgaggaaa tacccgagga
     2161 cttttcgctt ttgtatacga aaaaagattc acagcaataa aaccagtaaa tcctaattgt
     2221 cggatattct tttcgattaa taacatactc aaatcattcg taaatggttc tgtatctgtt
     2281 ggacgaatag taatgactac cgctaattta tcttccgctt cttttccttt gatcgcccat
     2341 tgtcgtttga aaacatagcg atgggtatga tgaggatctc gccaaacttc tgtttgtgtg
     2401 gtcactaatt ctttttcaat catctctttt tctcctatgc taatttttgt tcaagcaatt
     2461 caatcaccat tgccgttata agtgtcaata ctagttgaaa atatttcata ttctttctcc
     2521 ttttttcttc agaatttgat gaatcgctgt aaggatatct tctaaacatt tcgcaatcat
     2581 tctcaaataa tcgatcttat tcattcgttc ctccaatctg actccaaacc aatcgtcctt
     2641 ctaccagtaa cccacgaaca aattctcgct ggctggtaat atctgtaatg aaataaacta
     2701 catagtcttt tccatcttct ggtgcgcaat aaatataaag atgtgtcatc actaaacgtg
     2761 gtagagtaac atcttcagga agcataccca tttcaatttc ctctttttcc atcgtaacgg
     2821 attcatagcc ccactcttca ggagtagtga ctagctgtgc acacttttcc agtacttcga
     2881 tagccgtttc taacaagtcc tttcgttcct ttcttttaga tatcagtatt cttctggtaa
     2941 taacatcgtt tgatactcac ccgaatcaat gacccatatt ttttgagaca ctgatttcaa
     3001 acgaagctta ggtgaaatca gatactcttt tcttgcttct ggttcttctt gacgatgaac
     3061 caacttttga tttccttttt ccgtagaaaa ttcaaagatt tgaagatagt ctaaatgaaa
     3121 ttgcttttga acgttttgat cgattagatt ccaacataaa gcttgtaatt ccaccggcaa
     3181 actgtccatt actccctttg tgacataacg ttgtccttta ttttgaaaca tttcttttcc
     3241 ctcctttgcc ataaaaatag cccaatcttc tattaaagaa gattgggctt catggatata
     3301 atatatattt gatattcaat ttgattcatt ttatactatc tgatttgtca aattaagcta
     3361 gacaatatgt ctttatctat gcagctcaaa aatacttcca atggtgttcg ataatttaat
     3421 gattttcttg gaatatggtt tcttttcgag gcaacggatt gaataaaggt ttcatccgtg
     3481 tccctaaagt ccattgtttt aggtaagcca tctcgtctaa gcaagccgtt tgagtgctca
     3541 tttagccctc tttgcgaagg tgttcctgga tctgcgaaaa agatggcaat atcgttcgag
     3601 ttacttatag atcgccagtt ggaaaattct tttccacaat caaatgtcat ggatttaaac
     3661 aagtgtttag gcatggattt gaaccaatca tttaacctgt tttcgatatc ttcggctcgt
     3721 ctgcctgtcg gtttcaaagt aataataacc ttggataaac actcaaccaa tgtaataaca
     3781 gcgctcttgt gtttaactcc cacaatggtg tcgacttcga gatgaccaaa ttctttagtc
     3841 aggtgtggat agagactgtt ccgttcatga atcgttctgc gaaaggcctg ttttccacgc
     3901 ttttcttgat gattattcgg tttacgtttc cccttcatag gtaacgactg tgtgtcaaaa
     3961 aggccttttt taaacatccg atagagtgta cgaacagaac aatcaattgg aaactcgcca
     4021 cggccgataa tcacatcggg tgaccatcct tgagctacct tttcttgaac atatgttgat
     4081 tgttcatcag ataaaacggt tgaacgtcgt ccacaacgag atttattttg tttgtattga
     4141 cggtaataat ctagcacttt ttttccttgt ttgagaaacg aataaacatt ataaatggtt
     4201 tgtctagagc gatttaaact ttgtgcgaca agagcgacag gtttctcgat attgaaataa
     4261 gactctatga ttactagttc gtctgttgta agatgggtgt aagtcatttg tactcactcc
     4321 ttattaattc tttggtcgga actattttga gtgtagcaca aatgatcttt ttagttgtct
     4381 agcttaattt tacaatcgac gttttaataa attgtgatat aatgaagtat aactttttag
     4441 ggagtgtact atgaattttc aagataatag accattatat ttacaagtag aagcttctat
     4501 acgtaacgat attcgtgaaa aaaaatatct tccaggagag caattaccta cagaagaaga
     4561 attatgtaaa atttatggag taagtaagat taccattcga aaagctttta aacttttagc
     4621 agaaagtgga acagttgaac gattaagggg gaaagggacc tttgtaactc aaaaaaaaga
     4681 aagtttaaaa ttagctggag ttcatggatt tagcgattct ctttctacc
//
LOCUS       DACDHJ010000171         4724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDHJ010000171 DACDHJ010000000
VERSION     DACDHJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.174"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(56..1204)
                     /gene="nagA"
                     /locus_tag="IV223_002178"
     CDS             complement(56..1204)
                     /gene="nagA"
                     /locus_tag="IV223_002178"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8712437.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1280..2116)
                     /locus_tag="IV223_002179"
     CDS             complement(1280..2116)
                     /locus_tag="IV223_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712438.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2300..3517)
                     /locus_tag="IV223_002180"
     CDS             complement(2300..3517)
                     /locus_tag="IV223_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8712439.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3900..3983
                     /locus_tag="IV223_002181"
     tRNA            3900..3983
                     /locus_tag="IV223_002181"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3934..3936,aa:Leu,seq:gag)
     gene            complement(4223..>4724)
                     /locus_tag="IV223_002182"
     CDS             complement(4223..>4724)
                     /locus_tag="IV223_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712440.1"
                     /translation="ILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
BASE COUNT         1423 a          992 c          764 g         1545 t
ORIGIN      
        1 aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa ttatattatg
       61 cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca gggttcaata
      121 cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg attttacagc
      181 tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct aatccccagt
      241 ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag ttgccagatt
      301 ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg taatttccat
      361 caggcatccc accggccatc atacagtctg taattaatgc gacatgatca tgtcctgctt
      421 tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa atcaattcag
      481 aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga tgattcaatc
      541 cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca gctttttgcg
      601 cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct tcagtcactt
      661 gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt ttgattaaac
      721 cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg aaataacttg
      781 ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag attcctttga
      841 ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca tcttttaaac
      901 gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa gataacagac
      961 cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga ttcatatagc
     1021 cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg tcaatgatgt
     1081 tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg gtaatttcaa
     1141 gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag ataaaagttc
     1201 tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta gagaagaagt
     1261 caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct tgtgctaagc
     1321 cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag cttttaagca
     1381 caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag attccttgat
     1441 tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt ccataattat
     1501 aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc agcagttgga
     1561 tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca tatatttttc
     1621 cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct ttttggctag
     1681 agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga cgcaacgtga
     1741 attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga tacagatttt
     1801 cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt ttttggaaac
     1861 aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga gagagattca
     1921 tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc aatacattcg
     1981 gcagttcctc attattttcg atccgactta atacactttg tgaacaaata tcatgcgaaa
     2041 gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt ccaaaggact
     2101 cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc tattcgtatt
     2161 ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata tactttagtt
     2221 atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg
     2281 aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct ctcattttct
     2341 tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa tgatctggct
     2401 ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca
     2461 aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt gggaatatat
     2521 aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt gcattataga
     2581 tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg acactttgat
     2641 aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac aaacgcatgg
     2701 aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct ccacttagga
     2761 ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg ttcagcgtaa
     2821 caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca tccatcacta
     2881 agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct acttcaacga
     2941 tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga ttaatgataa
     3001 caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca ggataccatt
     3061 ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca atagaagccg
     3121 tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc agcaacccct
     3181 gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca ttgatcgtca
     3241 cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca gaaaagatcc
     3301 ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca cgaatgactt
     3361 cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa
     3421 tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca tcgtaactga
     3481 caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc cctcatttca
     3541 aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata ctccatccta
     3601 gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa acaagcgttg
     3661 agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat tgcaattcca
     3721 caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc aatcaataca
     3781 cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt aataagtcct
     3841 cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt tattattacg
     3901 cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt tagctcgtgt
     3961 gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt cacgagtttt
     4021 tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt acaaaaagaa
     4081 cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa gctactcgta
     4141 gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc acctaatact
     4201 aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag gtacaaatac
     4261 caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg cttttcatta
     4321 gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata tagtacgtcc
     4381 gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa caaaatccca
     4441 ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt aaataaattc
     4501 caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa gtacctggat
     4561 aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta ctactactcc
     4621 aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt gtattaccaa
     4681 aataaataat caggtttaag aaaaacggta atgcagtcaa tata
//
LOCUS       DACDHJ010000172         4656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDHJ010000172 DACDHJ010000000
VERSION     DACDHJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.175"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            349..1626
                     /locus_tag="IV223_002183"
     CDS             349..1626
                     /locus_tag="IV223_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8712441.1"
                     /translation="MEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGD
                     GKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGG
                     YPSELKTDAEKRAWRIYDILCNNGFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYG
                     LVQWDGSSYPLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGV
                     VQPTTVDGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGE
                     AGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGI
                     AYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQ
                     TYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            1639..2547
                     /locus_tag="IV223_002184"
     CDS             1639..2547
                     /locus_tag="IV223_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8712442.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            2547..3167
                     /locus_tag="IV223_002185"
     CDS             2547..3167
                     /locus_tag="IV223_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712443.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            3171..3620
                     /locus_tag="IV223_002186"
     CDS             3171..3620
                     /locus_tag="IV223_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712444.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            3620..4114
                     /locus_tag="IV223_002187"
     CDS             3620..4114
                     /locus_tag="IV223_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712445.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            4128..4559
                     /locus_tag="IV223_002188"
     CDS             4128..4559
                     /locus_tag="IV223_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712446.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            4561..>4656
                     /locus_tag="IV223_002189"
     CDS             4561..>4656
                     /locus_tag="IV223_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8712447.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         1637 a          683 c         1054 g         1282 t
ORIGIN      
        1 tcaatgatta caggtcaagg cagtgcagga ggatatgtag ttttatggcc aaaagaaaaa
       61 ccttccaaca tctttattat ggataatcca gatttagaaa aggcaagaga agtactaaga
      121 atgaataaaa atgggattgc tttttctaag aaaggatggc gggggccttt taattcagct
      181 tggacattag atagtatatt taatgccaat tttatacaaa ctggattaat caaagcagat
      241 atctttcaaa attcttttaa caagactgga gatgtattga aattagttaa tggactactc
      301 caaatttgga acaacaaaaa gaaaataatg gagttaacta aaaaaggaat ggaattttgg
      361 aattctaagg gttcaattgg aacgattgga acaactgatt ctgttggtaa tccttttcct
      421 ggggcttcta ctccaacccc tattgaagat aattctttag ttattcgtac aaatggagac
      481 ggcaaatata ttttgatttc tcctaaagta ggaaaaggat tagttttatt aggaaatggt
      541 aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa aggaaaagaa
      601 ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg tgggtatcct
      661 agtgaattaa aaacagatgc agaaaagaga gcttggagaa tttacgatat tttgtgtaat
      721 aacggattta cgaaacaatc tgcatgtgga atattgggga acattcaaca agaaacagga
      781 ggaacttttg atcctgatac tgttcaaata ggtggaccag catacggatt agttcagtgg
      841 gatggttctt catatcctct tgttggtcca gcaacatggg atggaaaagt ttatgttcaa
      901 aacttattta atgctgcagg tattaaagaa ccaataacga gcttagatgc acaagttcga
      961 ttgcttattt ggacatttac aaatggacaa tggatgggcg tagtacaacc tacgacggtt
     1021 gatggattta aggcctgtac tgatccaaga caagcagcat atgcttttga acgaaactat
     1081 gaacgtccgg cagcgacaca tcctgaacgt caagattatg cagttaactg gtataacaaa
     1141 tttaaagatt taaaaccagg aggagctact ggagaagcag gactaaaaca tttagagtct
     1201 ttgattggac aacgaattgg caatggtcag tgttatggct tgtctgcaga atattcagga
     1261 tatctgggtg gttgtggcat gggtgctgga acaaaatatg gtttaactca tgtgatagga
     1321 aatacttctg cagcatctga tattggtatt gcctatgatt ggtctgctgt tggttggaaa
     1381 gtgattcaaa accctagata tgatcaatta gtagttggtg caattattaa ttgggcaaga
     1441 ggtggacaag tgggttcatg gtttgcagat ggaacttatg gacatactgg cgttatccga
     1501 ggcttagcta atggtcgtat gcaaacttat gaacaaaaca cagagttagg tatgatttgt
     1561 ggaaagttag atcgccagta ttatagtgca agtgcaattt cttccattgt cataccacca
     1621 aaatagggag gtgatttaat ggcaaagtgg aatgtcatat taagtacaac agaaccctat
     1681 aattacgtgg ggatgattca agttcgacaa ggcaataaga atacagaggt tatggaagcg
     1741 actatagttg aaaatggtct tccctacgat ttatcagaat gtaaggtata ttttgaatca
     1801 gttgtaggtg ggaaatatcc agtccaatta gaaacaaaaa ttgtggatgc taaaaaaggg
     1861 aaaattaact atatttttga taaatattcc atgcagtgtt tacatcgaca aacagccaat
     1921 ttcattatat ttaaaggaga agacttgatt ggaacaactc aagacttctc ttattttgtc
     1981 attaatgctg tttcaaaaac agaaggagaa atgggttctt attggcaatc aatcgaagat
     2041 ttaattgcgg atatgacgga ctttattaat gaaaataaag gcgattttac ggactggatg
     2101 aatgaaagga aagaagaatt tgaacgttgg agacaagaac aggaacacag tttccaagat
     2161 tggagagaag gacaagaatc agactatttg gcatggtttg aatcaatcaa ggatatttta
     2221 aagacgattg atccaggtgg agtcatgtta gccgaactaa cggatgcgcg agtagatatt
     2281 caaggagttc gtcatgcgtc tatttcagag cgtttgctgg cagatatgga ctatttatat
     2341 cagaaattgc gagcaacgct tttcacgatt gaatacgggg agattgaagt gactgatatt
     2401 ttgcaggatg atctcttttc agataatcat gaagtcgaaa aagttggaac tgtagcattc
     2461 ccgattgaag aaggagcctt gatcatcgca accgttgatg atccaaagca aaatgtgttc
     2521 actcttgaga aagttggggt gatctaatgg ctaaaactaa acgaatgatg gaaacggatg
     2581 aaaaaacggg tgtacaacgc caattctttc caatcacaca tgcttccgca gttcttgggt
     2641 tagaagaaat aatagcagga gaagcaacag ttttatctgt taatggaaaa attggagccg
     2701 tcgtcattac taaagaagat ttgggattag agaatgttct cacagaatta ccctatgcaa
     2761 gtgaagaaga tgacggcatt atcacggcag aaatgtatca aaaaatttta aacagtggag
     2821 aaggtgacta cgtgttgcca gttgccacta tcgaccgttt gggtggcata aaagttggtg
     2881 aattattgac gattgatgag acagggaaag tctctgcagt cagacaatct gatgtcaatt
     2941 tttcgctgga gttaaaagaa aaactcgatt cactgaaaaa ttatactgct ggagaaaaca
     3001 tcactattga tgaagatgga acaattagtt ctacaggtgg tagtgggact ggtggtgtca
     3061 atcagtcata tgtagatcaa aaattccaag aagctgtaaa tcaagcagaa aattacacaa
     3121 acgaaagaat tccaaatttt acttttgaaa aaattgggga ggtatagaga atgacagata
     3181 tcgtaaaaat aaaacaaagc aatgtacagg tttatccaca aactcattgg aatgctatag
     3241 aaggcaaacc aacaacggtg aagggagata aaggcgatcc tggccaagca gcaacaatta
     3301 ccataggaac cgtttctagt ggttcaaccg cttctgttac gaatgtagga acatcatctg
     3361 cagctagatt taattttgtt ttaccaaagg gagataaagg tgatcctgga ataaatgcca
     3421 caactacagc tgtagctaca actacagcaa atgggttgat gtcgtccaca gataaaacta
     3481 agttggatgg aattgcagct ggagcacaga aaaatccagg taatgctaca actacgacag
     3541 caggtttaat gtcagcaact gacaaagcta aactcgatgg attagccaat attacatttg
     3601 agaaagtggg gacggtttaa tggctgatat tgtgcaatta aaagaagacg gagttgctaa
     3661 atacttaaaa acgcatgtag aagctattga tggtaaagaa gcattggtgc agacagatgg
     3721 agaccaagcc attgcaggac ataagaattt ttcaggttct gtaacaatca ataataaacg
     3781 tgttttgacg actgatgata acagatatga agtggtaaat ctggttgtca ctaacggtaa
     3841 tacagggaca gcaaagcttt atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt
     3901 aaacggaaaa agtagtggcg ggaatgattc ggttatttta actgttccag aaggctatcg
     3961 gacaccaatt agttttgaac aactggttgg gtcaatagac cgttctactt tgaacagtgc
     4021 tcaactatct attggcgcag acggagccat taaatggaga agaaattcaa gttatggatc
     4081 agcttattca tttgttatca cttattcaat ttaagggagg aaatctaatg aaagtagttt
     4141 acaaatcaat caagccttac ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc
     4201 ctgaaaactg tacagagatt aaaccaccag ttcctaactg gagaccaaga tttgattttg
     4261 ataaaaaaca gtgggttgag ttagctactg aagaagagaa aaaagggaat gctgttgata
     4321 gcgtagatga attggctaat attaaggcat tatacgaaac gctgaaggca gaaaatgatg
     4381 aattgaaaca gctaaattct aaagcaatgc taaacaatgt agcaatcaag caagaaaatg
     4441 tcttattgaa agaaaagtca gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt
     4501 ctgtacaaaa taccaaggaa atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac
     4561 atgtattcat atgaagatat caaactaatg tatgactggg gctgtttcac taacgaacaa
     4621 gtaatggttt tcgttccgtt gtgcattact gaagaa
//
LOCUS       DACDHJ010000173         4602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDHJ010000173 DACDHJ010000000
VERSION     DACDHJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.176"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(35..322)
                     /locus_tag="IV223_002190"
     CDS             complement(35..322)
                     /locus_tag="IV223_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8712448.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(342..566)
                     /locus_tag="IV223_002191"
     CDS             complement(342..566)
                     /locus_tag="IV223_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8712449.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1206..1739)
                     /locus_tag="IV223_002192"
     CDS             complement(1206..1739)
                     /locus_tag="IV223_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8712450.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1760..2704)
                     /locus_tag="IV223_002193"
     CDS             complement(1760..2704)
                     /locus_tag="IV223_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8712451.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3341..3427
                     /locus_tag="IV223_002194"
     CDS             3341..3427
                     /locus_tag="IV223_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8712452.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3931..>4602)
                     /gene="tnpB"
                     /locus_tag="IV223_002195"
     CDS             complement(3931..>4602)
                     /gene="tnpB"
                     /locus_tag="IV223_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8712453.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT         1292 a          835 c          720 g         1755 t
ORIGIN      
        1 ttcttcagtc ccattttcat tttattccaa agaattaact ccaaatacca atagaagccc
       61 atcctctata tacttgagat tccttactaa caatttcttg taattctatt aaagatttag
      121 gtaatccaaa tttatgtttt aaaatatatc ttccaagaaa actacttaat ttcatactaa
      181 cttcaccaac actttttccc tcactgattt ctttagaata aagaaagagc ttttctttta
      241 agtcaggata gtcatctata gttgcttcat tgtatacttt actaattgca tttaaaagtt
      301 tttcttcttt agtaaaatcc attataataa cctccctacc actaccttct aggtgcccat
      361 ggaccatgat taacccaacc attaacaata atttttccaa tttctccttt agcttgattc
      421 caatctaccc aacatttttc tttgttacaa taaagaccat ttccatagta agtagctgca
      481 tcaactttta ttcctgtacc gatgccagct agacaagttc ctaatattcc tagaataaca
      541 caatgtttta atactttctt tttcatgatt gtttttcctt ttcgtttttt taaaattgct
      601 tgttatatta aataatctta gtaattttat tatataacat tttattatat aacaatgatt
      661 aacgtcataa aaagtagttt tgaattcata atcgtttaaa taaaattata agatcagtta
      721 tttaaacagc tagtagacat aatgtcgata ctaccaagta acgcctgtac caagggatcc
      781 aggcgctgat tttgtgaatt tttattggtc taggttaaaa gctcttttta aggcttggtt
      841 ttctgcggtt gtttctttct aattctttgt gaatattgat cggtcatttt ttaaatactt
      901 gttatttttg tttgtgtttg ttatgctttt tacatatctc atattcattt attattcttt
      961 tctaaggtta ataataggat aatctctttc agaaagactg ctcgtacgag cagtcttttc
     1021 ttatttatca agtttttcta caaatacttc ctcattttga ctgttgtagt aattaggatt
     1081 gagttttagt tccccttttt tatttccttt gttggccact gataataaca ttgggctaaa
     1141 gcttaatatt cctagtagga tagaaccgaa tagcaagtgt ttttttgtca taagtatttc
     1201 cttcttcatt tccatagctg ccaccatttt ttccaattct ttgtttgttc gtccattgtt
     1261 tctgttgctt cagaaagagt cgttgaacta ttctctgaag actcttccgt ataagtcaac
     1321 tgcagttgct cctgaagttt ttcgatttgt tggttggctt gcagggtgag ttgttgttgc
     1381 tggtcgagta gtttttgtat atgagaatta ttttgctctt tgagggcaag ttgttctcgt
     1441 aaaaattcat tttcttcacg caactgatct agcaacttgt ttgcaaccac gtttttgttt
     1501 ggtatataag catttttatc gttgttttgt tcatgtttat gcaacttatc tagcaacctt
     1561 tgattgtcta agttgcgagc aagttgcaat actccagaac gcaatacaac cttataacca
     1621 cgctcatttt tagctatcat ggaagaatct aatttttgga tatgtttcga tattgcttgc
     1681 ctactcatct taaaaagatc tgacaattcc tttattgtaa aggtttcttg tggttgcata
     1741 ctggttgcct cctaggttgc tactggaata aattatctaa atcagataat aaactttttt
     1801 tctctttttc cgaaagtgcg gactctgttt gtttttcaag agcttttttc tctttttcta
     1861 gttgtttgat ttgttggagg agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa
     1921 ttgttgtagc gggttcttcg tattcttttt gtttatcagc taaagccaat gctttatttt
     1981 ggtcttcttt ggttggtgca tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga
     2041 acgtaaattg atagccaata acaggttttc cccgtccttt tccgtatttc tttttgattg
     2101 ttagtccttt gaagattgca gttagttctt gacgtatagg ttttataact cgtttatcta
     2161 tatcaggttg cttataactt ttaggaatat ctaataattc aagaaagtct tctttggaga
     2221 aatatgcgaa acctttggtt ctgtattgct ttaacagacg aaacatagtc tttgcatagc
     2281 cactttgtag ctcgttgaat tgttgtaaag aatagcgtac ccactcgtct aaattattta
     2341 acaaaggcag tgctttttcg taaattcgta tatctacgta aggattttct acgtccccgt
     2401 tgattttaaa ttctgtaaac attacaaaca tatcgtaaga aagcccagac tcacttctac
     2461 gaccaaagcg taaagataag attttttcat aagttgcttt taaatcttct acaaatcgta
     2521 catttgcagt tgctttgtag ttacttaaat cttttagttg gtcaaacgta aaacgaactg
     2581 tcttatcccc tttttcacgc attctagaca cgatggaaaa aaacaaattc atttcaaccg
     2641 gtgtaaattt tcttaaagga attgtattta attcatggtg gtattttaca atctcattag
     2701 ccatataatc acctcaatcc aattatgaat gacatttttt aaatgtcaag acattcatat
     2761 ttgacgttca aacagacaaa atgtccttca taaacagaca aaatgtcatt cataaacaga
     2821 caaaatgtca ttcataaaca gacaaaatgt ccttcataac attctgtatc cattggtaga
     2881 gtaaggacac agcttacggt aaagaggttt aaaaagaggt ttaaaaagag atataaaaag
     2941 aggggggcta attttcccgc ctaaaaacca cttctttttt ctttaaacag gttcgcgcat
     3001 ttttcaaaac tgccaatcca aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct
     3061 acgctctcac attgtactgc gcagtcgcct aaaggctcct ttgctaaaaa catgttggta
     3121 aagaggcctt tgagcggttc tgaatgctta tctaagccgt tttaagacag tcttggataa
     3181 aaatccttat ctagctttaa agcgccttac aggcggtcag ggcaagccct aacaacccta
     3241 aaataagcga tacttaaaaa caccacacaa gtttaatttt ttaagatata ataacttgtc
     3301 gcctatccga cagtcttgcg actggaagga ggtgtatcta atgtttgaat tgttcatttg
     3361 cccccttctt atagaagttt gtgtctcttt attcagttat tggttgaata accgaaacaa
     3421 gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag ccccagagaa tccgacctct
     3481 ctggggcttt cgtgtatcta atgtttgatc atttgcatgt ttagtttaac atgattgccg
     3541 atcaaattca attttgctgt tgctgatcta accattcctt gacacttttt agataattta
     3601 atgccgtttc ttttttcgtt ggattttgta tgttcgttgc ttgctgaagc agtgctttat
     3661 aacgttcgta ggtaactgat tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag
     3721 ggattgtatc actgaataaa ttataacaag cttcttctgt ctgcgtatct gtgaactact
     3781 caccacttag ctaaacctga cggttcttaa cgcttgaagt gggagcttct tgggaataga
     3841 gcatacttga tagcgtattt ctttactaac agcggtgtct cttatttacc aagctatccc
     3901 cgtagttcct acggttcttg atgattggat ctaagccaat cccatagaat gtagtcttag
     3961 accttcggtc agaatattga tactagcatt gatatcacga tcatgatgag tatgacaaac
     4021 aggacaagtc cattctcgaa tttcaagaga tttcttgcca tctttatgtc cgcactctga
     4081 acaaatctga ctagacggaa accatttatc tactttgatg atttttcgtc cataccagtc
     4141 agccttgtat tgtaatttag tcacaaactt tgaccaagaa acatcagaaa tacttttagc
     4201 taatttatga tttcgcaaca taccttttgt gtttaagtct tcaatacaga taatatcgtg
     4261 atttttgatc atttctgtac tcaacttatt cagaaaatca gtacgttgat tcattacttt
     4321 ttcatgcaat ctagctactt tgcgtttttg tttttgataa tttttagctt caaatagatt
     4381 ggtaccttta tttttagcca ataaagcacg cctagacaat ttgcgttgtt cacgctttag
     4441 tttcttttcc attttggacg tgaatttatt attatcaatt ttttgtccat cagaaagaat
     4501 cgcaaagtcc ataataccta agtcaatacc aattgtggaa ttagttttag gtaattcgat
     4561 aatttcttct ttacacaaca aagaaatata gtatttaccg ct
//
LOCUS       DACDHJ010000174         4575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDHJ010000174 DACDHJ010000000
VERSION     DACDHJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.177"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..1347)
                     /gene="dltD"
                     /locus_tag="IV223_002196"
     CDS             complement(79..1347)
                     /gene="dltD"
                     /locus_tag="IV223_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8712454.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(1350..1583)
                     /gene="dltC"
                     /locus_tag="IV223_002197"
     CDS             complement(1350..1583)
                     /gene="dltC"
                     /locus_tag="IV223_002197"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8712455.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            complement(1628..2833)
                     /gene="dltB"
                     /locus_tag="IV223_002198"
     CDS             complement(1628..2833)
                     /gene="dltB"
                     /locus_tag="IV223_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8712456.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(2830..4344)
                     /gene="dltA"
                     /locus_tag="IV223_002199"
     CDS             complement(2830..4344)
                     /gene="dltA"
                     /locus_tag="IV223_002199"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8712457.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(4355..4504)
                     /locus_tag="IV223_002200"
     CDS             complement(4355..4504)
                     /locus_tag="IV223_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8712458.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT         1431 a          924 c          764 g         1456 t
ORIGIN      
        1 gcaaacttgg tatttttatc ttttattcag aatattcaaa aataaagaaa acaagcgagc
       61 aacactcgcc tgttttcttt attttaattg tagtttatct ggtatttgat gttgccaaga
      121 tttgctaaaa aaagcatcgt caagatgata cttactagca gtaatatgat tttcttctaa
      181 aaacggtctg atctgttgat ctgcagccaa ccagcctttc cagcctagat ggatcgtgtc
      241 ttccataaaa tagttggtac ccgcttgatt gacaaaatca gctattcgat tgaatccttg
      301 gctatttaat tgaaatttga tctttttggc aaatccttgt aacatttctt gagatagtcc
      361 ggtataatcg ctccatttct cgttaaccgg tgggatgatg aacaatactt cgttatggtt
      421 ttttgctaat tgatccaaga ccagctgaaa atcagaaaat tctggcgaaa aacgataatc
      481 ccaatttttt tgtgactgtt tgagctctgg ctcgtgggct cggatacgat gcgtataaaa
      541 atcgtttttc aacccgaatg gattattcgt cgtttctcgt tctccaattt gatttgctaa
      601 aatggataat tctgtttctt ggtattgttt tggtaatcgt tttgactcat gatcgatttt
      661 ttgctgtcga tctttcaacc caatattgct gaatacttca tcctctctct tcagcatgtt
      721 ccattggctg cgcagctgat tcaaagattc aggtaaaggt agcttccctt ttttgatcgt
      781 ttgcagaatc gccgtcaacg tatcattctc ttttactttt gagaaagtta ataatcttct
      841 agctaaatag cgatcagcag gcgttacttt cttcattgaa aacaaccagt cgtatgtttg
      901 aagttcagaa taatacgtat taaagtaatc agtttttaca ccatttttaa cgaaccactg
      961 aggagaaata ataaagacga ctttcttgtt tttcatagca tctcctgcag aacgcatcat
     1021 catatattgg cttaacgatt gtgtcccggg tgctcccagc aaaaatggac gatagtttcg
     1081 ctggtatttt tctgccagaa cagacggatg aaatggactg attctgctta attcacttga
     1141 gccaaaaaaa ggaatatatt gtttttcaga taatgcataa ttttttatcg catctccttt
     1201 caacacgttc actgacatgg aagaagaggc acgtctaatc gtttctggat tagagatatc
     1261 tacttgatag gaggaggtaa aaaacacaaa cagtaagcca acagctaaaa caaccgggcc
     1321 gaaaatagca aaaagtttct ttttcatact caacccttca atgcagttgc ttgttgaatg
     1381 attttattcg gtgtgctcca atcctctcgc tcgaattcag atacagggac ttggatatcc
     1441 agctctccgt ctagctcgac taataattga acagatgcta aagaatcaag caacccttca
     1501 tcaaatagat caatatcacg attttttctt acttcatctg ttcctgttat ttcttctaag
     1561 attgataaaa tttgttcttc cattttcctt ttctcctttg ttatataaat taatatgtca
     1621 ctattcgtta gctaaaaaaa agtttatcta aaaatcctga aaaaatcagg aagctaaaac
     1681 atactgcttg aaatgtcaaa aatacggcta agccatgcgt aaatttgtta gaaggcaact
     1741 gctctttatg cttcttctta aatcggatcc atgcatctgt cagacagatc agacaagcat
     1801 gatacagtcc atatacgatg tagtaccacg tcaatccatg ccatataccc attagaagaa
     1861 acaaagcaaa ataaccgata tttgacatta cgatcttgct tttaaaaaat tttttcttca
     1921 tcatgaagaa catcaatcgc atatatacat aatcacggaa ccaaaaggac agcgtcatat
     1981 gccaacgatt ccagaattct ttgatattcc atgataaaaa aggtttattg aaattgattg
     2041 gtgtctgata tcccatcatg tagcttgtac cgactgcaaa taagctatag ccagcaaaat
     2101 caaaaaacaa atacatactg tacacgtaca tataacctac taatgcccat gaaataccac
     2161 catgctgtaa ggcaatgcgt cccactacag gaagcagtac ttgaccaaaa taataaccga
     2221 taatgaattt atataaaaaa ccgacaaaaa tattttggac gccttttccc aaaagatcaa
     2281 catatttatc acgaacaggc ggatggagct gatctttttc aaatctccta tagcgatcga
     2341 ttggtccaga agagattgtt ggaaagaaca gcagaaactg gatataacga aatggcttat
     2401 atgttttgat catgccatct cttatttcca tgatcatttg tacagattta aaggttaaat
     2461 aagagattcc taaaaaacca agtaacgatg cttttccatg ttcaaaaaaa ggagtcactt
     2521 tcgttaaaaa cagtggaatt atcgctaaaa taacggcggc aaaaaaccat cctgtctgat
     2581 ttttcctttg ccgataaaca aaatagcccc atacaagtat cgtctgccac aaaacatacc
     2641 caatcagcgc cgctccctgt tgccaataat tacctccaaa gctgacaaat aaaaagaaaa
     2701 gtgtcactgc tgattgataa actggtaatc ttttttccca tatcaataaa gatagaacga
     2761 tcggtacaag cgcaatggct aaaagaaaaa aataaaacgg ctctgcataa gggatcatat
     2821 gaggtaacat cattcaggat tcacctcagc tgttaatttt tttcgatcga tttttccatt
     2881 aactgtctga ggcagcagat cgacataatg gaacttctga ggaatcatat aatccattac
     2941 aacttctagt aattgttctt tgatagattt agtcaactga aaatccggct gtttcgtttt
     3001 gttttctaat acgacaaaag caaccagctg ctgtactttt gttccttggt attttggtac
     3061 gacgacagct tgtttgattc ggttgtcttg gagcagataa tgttcgatat ccccgagttc
     3121 aatccggtat ccatgtaatt tgacctgctg atccatacgt ccttcataaa ataaaagccc
     3181 atattcatct agtctacctg catcccctgt atggtaactt gctaagtcat tttgacaaaa
     3241 gaacgcagca gctgtctttt cgggattgtt caggtaacct tttgatacgc ttggtcctgc
     3301 aataatgatt tcacccgttt ctccttttga aacaggttta ccttcttgga caatagaaac
     3361 tttcgtgtct ttttttacgt agccaatcgg tagtcggtca taactatcta gtacatgctg
     3421 gtcaatctga atacttgaaa tagctacagt tgcttctgtt ggaccgtaag tattatatat
     3481 ccgtgcattt gggaatcgat caagtagact ttctgcagtt tttttcgtca attcttctcc
     3541 gcaaaataaa aacatgtcca attccggcac attttctcca ttgaatgtag gttccatcaa
     3601 gcaaatatcc ataaatgatg gagtagaaac ccacacattc aacttcaagg ctggcaatgt
     3661 cgcgaataat tgacgaaagt cttgaacaac tgctttattt aacggcttca gcattccgcc
     3721 agaaactaat gcaggataaa tagaaaatac cgataaatca aaagaaaatg gcgcttgggc
     3781 taagaaatgc ataccaggct tgatcccaaa gtcttccatc gtccattgaa caaagctgac
     3841 tagattatca tgactgattt gtacgccttt tggttctcca gtagtaccag aagtaaaaat
     3901 aatataaaaa ttttctgtac cttttacgct atttctggga taaaatccta ctggtatctt
     3961 cttgatttct tgaaattctt tttttgttat gacaggaacc gaaggaatat cttctatcca
     4021 gtccccgata ctgatgacca tagatggttt agccacccga aagatcgata ggatacgctc
     4081 cttgggtgta tgctcttcaa tcggcaaata ggcatggcca gatttgacaa cccctagaaa
     4141 tgagacaatc atttcaaact ccaaattgcc aaaaacgaca attggtccat cgccttctac
     4201 ctttttttct aaataataag caagtgcgtc tgatgcttgt ttcaattcac caaaagtatg
     4261 cgtctcatcc gcctgataag caactcgatc aggttcattt attccccaat catcgattgc
     4321 ttcaataatc gttttgattt ccatactctt cctcctaaaa ttcgttatag ataaaagttc
     4381 ctccttgaat atttttatag tgatacagat aaatcaggaa cagcagtact gcactataaa
     4441 aaattgttcg aatgatgaat tttccccaat aattcaattt tggattagaa cgccatttgt
     4501 tcattggatt ccctcctcat ataacctcac acaaagtgta actaattttg acaaaaagaa
     4561 acatgtgtct agctt
//
LOCUS       DACDHJ010000175         4519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDHJ010000175 DACDHJ010000000
VERSION     DACDHJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.178"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            239..469
                     /locus_tag="IV223_002201"
     CDS             239..469
                     /locus_tag="IV223_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712459.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            628..1461
                     /locus_tag="IV223_002202"
     CDS             628..1461
                     /locus_tag="IV223_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP8712460.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1454..2869
                     /locus_tag="IV223_002203"
     CDS             1454..2869
                     /locus_tag="IV223_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8712461.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            2881..4410
                     /locus_tag="IV223_002204"
     CDS             2881..4410
                     /locus_tag="IV223_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8712462.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
BASE COUNT         1635 a          674 c          943 g         1267 t
ORIGIN      
        1 gactttggca accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg
       61 ttttgtgcat cgtgtaggtt gctattacat aactggttag gttagattga gatttgggat
      121 tcggtacaaa tgaatcgtca aatgtctcaa gcacaggatc ggaaacgtcc ctgcctgtgc
      181 attacatatt agatcactct ttgagtggtc tttttcttta cataaaggag gctacataat
      241 gagaaactac tggtatgtat cgctaactaa tgaatatcct cgaaccattg atgattgttc
      301 agtgcgtgtt gtgcgttctg tacaaatcaa agggaagtac tctattgtcg aaatggggag
      361 agaagcagaa ccatgtgaga ttgatgcgtg caagctcgtt tattgcggtc atggtttcta
      421 tgatgaacca aacattcaaa ataatattaa caagaatttg agggattaga atgcaagaaa
      481 tggcttaaaa atctccaatc aggacaaatc aagttagaca aagtttccga tttaaagata
      541 ttgattgaag ctgatttaat gctaaaagat attgaaaatt agaaaacaaa actcaaccta
      601 aacagaatgt gaggtggtgt gtattgaatg gcaagacaac gtgatccaag acgtgatgaa
      661 gccaaaagaa tttggttaga atccaacgga gaaaagcagt taaaggaaat tgcatctgaa
      721 ttaaatgttt cagattctca ggttagaaaa tggaaatcgc aagacaaatg gagcgctgaa
      781 ttgattagta acgttaccaa tggcaaaagt aacgttacta atcaaggtgg cgctcctatt
      841 ggtaatcaaa atgctaaagg taacaaagga aatagccgag cttctccgcc agtgggtaat
      901 aaaaacgctt tgaaaacagg tgagtatgaa acaatatttt ttgacacgtt aaccgataat
      961 gagaaggaca tctattctag tttgaatgat gatccttctt ttgctttgtc cgaagaaata
     1021 cgacttctta aaataagaca acttcgaatg atgaaaagaa tccaacaagc tgaagctgga
     1081 ctaaatgatg aagaagtcga acgattgcag cagctaagaa agattaaaac tccgattgaa
     1141 aaaaatggta aaaagctaga aatcaagcgt gaggttatgc aagatgtgca gattagcaga
     1201 aaaaaacatc gcaaaattga tgatattctt tcaattgaag attcattgac tcggattagc
     1261 aaccagttag ctaaagccat caagcaaatg aatgaacttt atatgaatga atacagaact
     1321 gatttaatta aagctcagac tgataagatc caagctgaga caaatgaaat tggcggaaat
     1381 aattcaggtg aagaaataga agaatggaaa caggcagttt taaatgccgc aaacaaacgg
     1441 gcggtgaaag aaaatgaata atgaatttat tccttttgct gatattggtt ctgccattga
     1501 ttattactat gataaaccag tagctttttg ccaagatatt ttgcatttga atcctgatga
     1561 atggcaagaa aatgttttaa atgatttagc tgaattttca aaggtttctg ttcgttctgg
     1621 tcaaggagtt ggaaaaacag cattagaagc aggagcaata ctttggttct taacgtgtcg
     1681 accctacgct aaagtaatag caacagctcc gacaatgaag caactttacg atgtactttg
     1741 ggcagaggta gctaaatggt taaatgatag cttgatcaaa aacttactga agtggacaaa
     1801 gaccaaaatt tatatggttg gtgattcaga gcgttggttt gctacggcta gaacagcgac
     1861 taaaccagaa agtatgcaag gttttcacga ggaccatatg ttgattgtgg tagatgaagc
     1921 ttctggtgtg tctgatccaa ttatggaagc tattcttggt acgctatcag gttttgataa
     1981 taagctgttg atgtgtggaa accccaataa tattgaaggt gtcttttatg attcccataa
     2041 ttcagaccgt gataaataca gagttcataa agtatcaagc tatgatagta aacgtacaaa
     2101 caaagacaat atagaaatga ttcttaaaaa atatggaaaa gaaagtgatg ttgctcgtgt
     2161 ccgtattttt ggagaatttc cgaaaggtgc gttggattca tttatcagtc ttgaaacagt
     2221 tgaattggct acagaaaaac aaattagtga ttctttagtc aataaaacaa aggttgctca
     2281 tattggtgtc gacgtagctc gatatggtga tgattctacg attctctttc ctagaattgc
     2341 taccagggca ttggagtatg agaagtattc aaaacgtagc accatggaaa caacaggata
     2401 tgtcatcaac atggccaaga atctaatgag tcaatatccg agtattgata aagtgatgat
     2461 taaagtcgat gacactggtg tcggaggtgg tgtaaccgac cgcctagaag aacttataga
     2521 agacaaacat tatccttttg aggtgtttgg agtgaataac ggttcaacat cagaagacga
     2581 tttttacgat aatttaggta ctcaactatg gggaaacatc aaggaaatgt tagaagaaaa
     2641 tatgacagca aatcttaacg gagaacagcc tgttattgaa ttgccttctg atagttcgtt
     2701 aatcaaagaa ttaagtactc gcaaattcaa aatgacaagt agaagtcgta tacgtttaga
     2761 aagtaaagat gatatgaaaa agcgaaatat tggtagtccc gatattgctg acgcactggc
     2821 tttagcgttt tatgagccac caagtcacta tcaatttatt caattttagg aggtgagctt
     2881 ttgattagta cagtattatc tttagagaga tataagaagc tacgtgttaa atatgcaaca
     2941 caaattgaag atggaatgtt tgatccgaat ggttttatag aagatatgaa accatttttt
     3001 gctgatcgcg aaagaaaata tctagcttat actagcgaaa agaatgaaat agataataga
     3061 ccaaagccta acactgatat tgtaaaagtt aataataaac ttcatgctgg tatgtattca
     3121 attgtcgtag accaagcagt caatcatttt actggtatac ctatcaaatg ggattacgat
     3181 gtatcggaac aaaaaagaac actcattcaa agattaaaag ataaattctt aaaaaatgat
     3241 atagaacttc cgacagttcc agaagctttt aacaaattaa caagcaatct tgattccatg
     3301 agattcgcaa tgcttgattc tgaaactgca acttttcaag gagcttgtgg agtagctttc
     3361 cgattgctag agcctgtgga agaagatgat ggttggaagt taagagcaag taatattgaa
     3421 ccttggagag cagaaagata tggaaatgcc ggaatctata tcaaagaaaa gtatgactct
     3481 taccagaaaa aattttttca agaaatgaaa gttatgacaa gaaataaaat tctgacatat
     3541 gcttgctatg gtgatttgaa ttttgttaca agcggaacgt tcaaaaaaat tgatgagacc
     3601 gataaccctt tggggacgat taccttggca gaatttaaaa ataatacgaa tcgttattgt
     3661 gattttgaag tagctgagga aattggagat gcaattgatc gtgctttgtc tgatcaacaa
     3721 aacgaaatcg aacaatttaa gcttgcgtac atgcttgtta ctggcacaac aatgagcaaa
     3781 ggcactgcta aagaaatgat gaatcagcta gggattatca acttaaaagc ccctactgca
     3841 aaagctgaat atgtcacaaa aaatttagca aaagatttca atgaatatca tatggatcta
     3901 ttgaaaaaac agttttacac catttgtaaa gcaatcgatt ttaacgatga ggtgtttaaa
     3961 tcaaacagtt ctggagaagc tcgcaagtgg caaatcatta gcctagaagc gaaaacaaat
     4021 actaaagagc aatattttag agaaggattg aaagaatgcg cagaaacaat tgctgctttt
     4081 cttaagtttc atgacaaagt agaaattgag cctgaaaaaa ttatttttac tttctctaga
     4141 tctttgccaa ctgatttaag ttatttagct gaagctttac ctaaacttgc accatacgta
     4201 tccaaacgaa ctattcagag ccaaattcca tttgtaacag atgttgatta tgaaaatgaa
     4261 atgatggaat tggaaagtgg aagtgcttat ccagatagtg aatacaattt tggcggaggt
     4321 ggcaatagtg acgatagaaa cgaagtattg gaccaaacgt cgagaactgg aggatcaagc
     4381 aaggctcaaa caagaaaatc agacacttaa aaaattaact agtgtatttc ctgaagcact
     4441 gaaagagata caagcaaaac tattatcaca agctgactta cacaatatca cttatccaga
     4501 aatgatggag ttttacagt
//
LOCUS       DACDHJ010000176         4464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDHJ010000176 DACDHJ010000000
VERSION     DACDHJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.179"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV223_002205"
     CDS             574..1008
                     /locus_tag="IV223_002205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712463.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2301..3224
                     /locus_tag="IV223_002206"
     CDS             2301..3224
                     /locus_tag="IV223_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8712464.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3240..3779
                     /locus_tag="IV223_002207"
     CDS             3240..3779
                     /locus_tag="IV223_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8712465.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          668 c          758 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaattatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttttata ctaggactgt cttaaaatgt cttaaacgaa
     1861 cgctcagagc ttctcaaagg gttccttatg aacggctttt agcaaaggag ccggaggcga
     1921 ctgcgcagta taatgtgaga gcagagcgat cacacctttt tagcgaaccg tagggagcaa
     1981 ggtttggaat tggcagtttt gataaattcg cgaacctgtt tagagaaaaa aggagtgatt
     2041 ttttaggcgg gaaaattagc ctcccctctt tttatatctc tttttatatc tctttttata
     2101 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
     2161 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
     2221 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
     2281 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
     2341 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     2401 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     2461 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     2521 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     2581 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     2641 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     2701 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     2761 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     2821 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     2881 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     2941 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     3001 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     3061 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     3121 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     3181 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     3241 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     3301 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     3361 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     3421 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     3481 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     3541 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     3601 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     3661 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     3721 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     3781 attttctaga agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc
     3841 actcaaattt ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag
     3901 ttgtaaacta tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa
     3961 tttttggtgg aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac
     4021 aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag
     4081 aattttgatc atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa
     4141 taaagtatgg gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc
     4201 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     4261 tgggaggcgg agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DACDHJ010000177         4456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDHJ010000177 DACDHJ010000000
VERSION     DACDHJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.180"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(91..612)
                     /locus_tag="IV223_002208"
     CDS             complement(91..612)
                     /locus_tag="IV223_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8712466.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            810..>4456
                     /gene="nifJ"
                     /locus_tag="IV223_002209"
     CDS             810..>4456
                     /gene="nifJ"
                     /locus_tag="IV223_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8712467.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKK"
BASE COUNT         1394 a          830 c          991 g         1241 t
ORIGIN      
        1 agaaggagtt gcttctgctt ccgccgttta taggtgttta gagcgtgaga caaaagtgtt
       61 ctttactttt gtcctacgct ccttattttt ttattttgtt tggatcaatt cttttaattc
      121 ctctattgaa atagattcat caaagatttc tttacccaag atgacagtcg gaacaaatcg
      181 aatatgtgct tgctcagctt cgttgatgac agcttggctc agctgttcgt ctttttgttc
      241 tgtcagcttt agtttatcta ctgcaaaatc cgctacttct tggagagaaa gatgtttcca
      301 ctcgtcttga gtatcgaata tttgtttgat ctctttgatc gctttttgac catcgctgat
      361 tgtgagatac cggtgcatca cgtttcctcg ctgcaagctt tctttttctt tatcgaacaa
      421 tttgatcact cgctgcaatt gcccgctttg gactgcttct tctaatagct cataagattc
      481 ttcaaaccat tgtcggcaat acggacaaga taggttgatg aattcgacca tttttttagg
      541 agcattcgat gaaccataaa ggattccttt ttgtgtattt gttttcgttg catcaatcac
      601 tgatatatcc atgttaaaac acctctttcg cttatagttt atacatgtct gacaaaaaaa
      661 ggcaacgatt tagactgttt gtctaaaaat tagatgacca attcaaatag acatatgtga
      721 aatattttta taaataaaag aaaaaatgga atgttttacg ttataatgga acagataata
      781 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
      841 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
      901 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaatgtat
      961 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     1021 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttctcaa gggctgcttt
     1081 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccatgttg
     1141 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     1201 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     1261 tagcacctgt tgcccatctt gcttctattg aaggagaagt accttttttg aatttctttg
     1321 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     1381 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     1441 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     1501 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     1561 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     1621 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     1681 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     1741 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     1801 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     1861 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     1921 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     1981 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     2041 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     2101 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     2161 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     2221 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     2281 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     2341 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     2401 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     2461 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     2521 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     2581 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     2641 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     2701 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     2761 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     2821 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     2881 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     2941 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     3001 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     3061 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     3121 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     3181 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     3241 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     3301 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     3361 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     3421 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     3481 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     3541 aagactggtt ggtacacatg tttgagagcg aaggaacgca gcaaagagca gcaaaactgc
     3601 aagctgcgat cgaaagcgaa cgatcaattg tccctgaact ggaggatata tacaagagaa
     3661 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     3721 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat attcttgttt
     3781 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     3841 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     3901 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaga
     3961 cgataaaggc ttttgaagaa gcagaaaagt atcctggacc ttctttaatc attgcctata
     4021 cgccttgtat cacacatggt ttgcttggtg gaatgaaaga atcgcttaaa gaagcaaaag
     4081 aagctgtttc ttctggctat tggtcattat atcgatataa ccctgcaatg gtcgaaaaaa
     4141 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     4201 tgaggaaaca agttcgcttt tcctcgctag agaacgcaaa tccaaatctt gcaaaagctt
     4261 tatttgaaaa gactatccaa gacgctaaaa cacgttttta caactatgct gcactgacgg
     4321 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     4381 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gccagagcag
     4441 aacgacgtaa aaaaat
//
LOCUS       DACDHJ010000178         4454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDHJ010000178 DACDHJ010000000
VERSION     DACDHJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.181"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            53..499
                     /locus_tag="IV223_002210"
     CDS             53..499
                     /locus_tag="IV223_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8712468.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            515..829
                     /locus_tag="IV223_002211"
     CDS             515..829
                     /locus_tag="IV223_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8712469.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            855..1904
                     /locus_tag="IV223_002212"
     CDS             855..1904
                     /locus_tag="IV223_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8712470.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            1916..>4454
                     /locus_tag="IV223_002213"
     CDS             1916..>4454
                     /locus_tag="IV223_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8712471.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLILIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALE"
BASE COUNT         1557 a          652 c          926 g         1319 t
ORIGIN      
        1 aacgagttcg tcgtttgaaa aattagttta tatattgaaa gaaggcttat aaatgatcag
       61 tgaaaaaatc aatataaata atatagtgat agatttaaat gcatctgata aaaaaagtgt
      121 agtcgaaaaa ttatcgaaat tgttagagac taatgggttt gtgtctgacc acaaacaatt
      181 tcaaaaagac gtatttgaca gagaaaacca tatgaccaca ggaattggga aaaatatcgc
      241 cattcctcat ggcaaaagcc tagcagtaat tgaatcaaca gtggctgtag ccaagttaaa
      301 gcggtctgtt gaatggggcg cattagatga tcaaccagtt gatctcgtct ttttgttagc
      361 aataaaagac actgataaag gtactaatca tttacgtatt ttagcagaat tatctggaag
      421 attgatggat gacgagtttg ttaaaagaat aaaagattcg agaaataaaa aagaattggt
      481 aaaagctcta tcattttaaa aggaggaatt tcatatgaca aaaatagtag cagtaacagc
      541 atgtatcgca gggattgccc atacttatat ggcaaaagct aatttagaaa agtttgcgaa
      601 gaagaaaggg ttagatatta aagtagaaac acagggggct atggggatag aaaacaaact
      661 ttcaatttcg gatgtttccg aggcagatgt tgtcatattt gcagtagata caaatgtttc
      721 agaacgaggt cgctttgacg gtaaaccgat tttaacagta gggacctcag aggtagtaaa
      781 agatggtgaa gagacgattg atgaagcaat tgcattaatt aacaaataaa aaatatatcg
      841 tgggggagtt actaatggaa ttcaagaaac aaggaagaga aattctaaat catttccaaa
      901 gtggggtttc ctatatgatc ccgcttgtcg tagcagcagg gttactaact tctattgcag
      961 tcatttttgg tggcacaggt gtttgggacc agacggatac attctgggga gtcctgcgaa
     1021 tgattggtca gacgggatta cagtttatcg taccgatgat ttcagcctat atcgcatatt
     1081 caattgctga ccgtcccggt ctagcaccag cttttatcac tgggatgatg tgtcaaaatc
     1141 ttggtatggg atttattgga ggaatggttg ctggattagc ttgtggctat ttagctaatg
     1201 cattgaaaaa agtaaaagta ccgatgcaag ttattagtct aaaatcaatt atgctgattc
     1261 cttttgttac tacattagtt gttggaatca tattatggta tgtactagct acgcctattc
     1321 aagcaatgac aacaggactg actaattggc tagaaggcat gtcaggagca aatgctgctt
     1381 tgatgtcggc tattttagga ggaatgatgg cgtttgatat gggaggacct atcaataaaa
     1441 tcgcatatgc ttttggggtt gcttcttttt caagcggaag ctttggacct agtacagcca
     1501 tgttattagg aattgctatt ccgccatttg gaatgttttt agcaacaatt ttaaacaaaa
     1561 aactttatga tgaatcggaa cgagataatg caaagacagc tatgattatg ggccttgtgg
     1621 ggattactga agggacgatc ccgtttgccg tgaaagatcc tttacgagta ataccaagca
     1681 tcgtggtggg cactgcagta gcatgtggtc taaatggatt ttttggtgtt actcaagaaa
     1741 caatgctttc aacctttatg gcaattcctt ttgttacgaa tataccttta tatttagtat
     1801 caattgttat tgggggagtg gttacagcgt taatggtcaa tgcactaaaa tccatcaagt
     1861 ataaaaaaga acaacaagta aattcagtag aagaaggaaa gtaggagaat ctgatatggt
     1921 aaatgcacat ataattcaac acacgcactg ggacagagaa tggtatttta caaatgaaga
     1981 tgccatcgta ttgtctgatc aggtctttac tgaagttcta gatgaattgg aaaggaaccc
     2041 taaagtaaac ttttgtttag atggacaaac ttcaatcgtc gatgagtatt tggagatcaa
     2101 tccgcaaaag cttaaggtta tccaacgact gatgaaaaca ggacaactat tcgtgggacc
     2161 gtggtatgca caaacagatg ccttattagt ggatgctgaa tcaattttaa gaaacttaat
     2221 gattggaatc agtgatacga tccttaaata tggagatcca atgatggtag ggtatttgcc
     2281 tgacacgttt gggttcaatg cgaatttgcc tacacttttg catcaagtag aaatcgataa
     2341 tttcatgtgt tggcgaggat taaactttga aacgatggtt ccatctcctt attttatctg
     2401 gaaaggattg ggagataaat ttgtttatgc catgaatttt ccatttggtt atatgactgg
     2461 aatgatgacc ttagacgcaa ttaaagatat tcctgaattt gcaaaagaaa agttggatga
     2521 agcagttgaa atgcttcata tgcaaggaaa aaacaaagat attttagtac cttcgggtgt
     2581 agatcaaaag tcaatgattt taaattttga tcaagtcgta gagaagttga ataaatacag
     2641 taaattccac aatattattt cagattatcc ttcttatgta gaagttatca gaaacaaaaa
     2701 agaattacct gaatataaag gtgaacttcg tcagccggtt tactctcgtg ttcatcgttc
     2761 tattggctca gtaagaacaa aaatgaaaat agaaaatttt aaattggaac aaaaaattct
     2821 aaaacgagtt gagccgctca tactgattgc agaaagaaat ggtatccata tcagtaaagg
     2881 cctacttcgt cggttatgga aaaagatttt gcaaaaccag gctcatgata gtattggtgg
     2941 ctgtgtttca gataatgttg cggaagatat tttccatcga atcaaagaag ctaatgagat
     3001 agctgacgga atcgaaaacc ttattgtaaa acggatggct gatgcattaa agctaaaatc
     3061 gaatgaagtc cttgttttca acacaacgac tgagaattat agtggatcga aaatcgtgca
     3121 tgtagtagct gattcaaaga aaatacactt caaaggtagt ccctataata tcattgaaaa
     3181 agaagtttat tttccagaac ggaaaaatgc ttatcggatg attgctacag gtttcgaata
     3241 ttttgatgaa ccagcttatt atgaacttga cataaaaata aatgtaaatg tgccagcact
     3301 aggatacact gttgttgaat ttgagaaaaa taatgaaaaa ttagaaacag caatcgagat
     3361 agacaaaact gagataagta acaataaata taaactttct tttaaagatg ggcaattgaa
     3421 tttgatagtt ggagatagac aatatcttga tttcgttcat ttgatcgatt cagcaaatga
     3481 tggcgatacg tatgattatt caccattaga aggagataca gaactatctc taaaattaga
     3541 aacagctaaa gtatataaag attctttaca agaaacatta gttgtttacg gaaaagctca
     3601 acttcctaaa gatttaaaag atcgtttatc tgaaaaaccc gaaatggaag aaatatctta
     3661 tgaaatatcg ttcagtctgg gagaaagtca aatcgttgaa ggcacactca aaattcataa
     3721 tactgtttac tcgcatcgta tgcgcttagc aattaatgtt ttagataaat ctgaaaaatc
     3781 aattgcacaa attcaaaacg gatttattga aaatacccct gatgtcattc ctccgaattg
     3841 gtcagaaact atggtagaga aacctgttaa tttagaaata ttcgataaga gtgtctctgt
     3901 ggcagaaaat gatcattatt ttacgtttta tgcagatggt ttaaaagagt atgaacgtat
     3961 agaaaatcaa ttaatcataa ccttattttc aacaacaggg gaacttggaa aacctaatct
     4021 tgcttggcga cctgggcgag cttcaggaga tacgacaaat gaagggcatg tcatgatgga
     4081 aacaccgcta gctcaagaga taggtgagta taaagtcact tttggattta atgtagaaga
     4141 aggaagattt aatgagttca aagtggcaaa acaggcagaa aaacggttag aacaatctat
     4201 ttcttatcaa aagcagaaac tgaatgtatt tattcacagg ttagataaca agatttggcc
     4261 attacaaaat gaacttgatg ttcctcgtaa attttcattg attgaattac ctgaagactt
     4321 aattgtatca tcgatttatc cttcttattt tcatgaacaa ggatttgttc ttcgcttagc
     4381 taatccaaca gaacaagaaa aaatagtacc tgaggctatc ctttcccttg ggacagttgt
     4441 caatgcactg gaga
//
LOCUS       DACDHJ010000179         4434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDHJ010000179 DACDHJ010000000
VERSION     DACDHJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.182"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IV223_002214"
     CDS             156..1862
                     /locus_tag="IV223_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8712472.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IV223_002215"
     CDS             complement(2020..2922)
                     /locus_tag="IV223_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8712473.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IV223_002216"
     CDS             complement(2919..3623)
                     /locus_tag="IV223_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8712474.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IV223_002217"
     CDS             complement(3654..4295)
                     /locus_tag="IV223_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8712475.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1370 a          948 c          764 g         1352 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaa
//
LOCUS       DACDHJ010000180         4434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDHJ010000180 DACDHJ010000000
VERSION     DACDHJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.183"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..905)
                     /locus_tag="IV223_002218"
     CDS             complement(<1..905)
                     /locus_tag="IV223_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8712476.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKG"
     gene            complement(907..2346)
                     /locus_tag="IV223_002219"
     CDS             complement(907..2346)
                     /locus_tag="IV223_002219"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8712477.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2531..>4434
                     /locus_tag="IV223_002220"
     CDS             2531..>4434
                     /locus_tag="IV223_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8712478.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVA"
BASE COUNT         1393 a          767 c          742 g         1532 t
ORIGIN      
        1 tcgcctttaa tttttttcag aagaatatca acaataagac ttgcaatttc atcaattggt
       61 tgtcttattg ttgtcaattg tgggaaaaat ttctcaataa aagaggtacc atcgtatcca
      121 ataattttta aatcttctgg gatattaagt ttaagttgat gagcaatttt catagtgaga
      181 atggctgtta aatcatcaga aacaaaaata ccatctggtt tgtgatttat aatagttgac
      241 tttatttcca tttcacgacg gatagtagac agattattag gaattttgaa aatttttcca
      301 tttggatttt gaaaagaaaa acctagtgca cgtaattctg tgggagaatc cgtgttatca
      361 tttcctgtaa tcataatggt attttgacaa ccattttttt gtaaagtctt agctgcaagt
      421 tttccccctt caaagttatc tgaagaaaca atggaaacat taggagctaa atttctatca
      481 aaagcaacaa taggtgctct cacacgctca taatcagcaa ttccaagatt atggcttgat
      541 gagattatgc catcaacttg gttggcttca agcatttcaa tgtattctct ctctttagca
      601 ggatcatttt gactattgca aataatcgtt ttatatccat gtttaaataa ctctatttcg
      661 agatactcaa tgagttcaga gtaaaagata ttactaatat taggaaaaat aagaccaata
      721 agctgaggtg actttccttg aagtccacgt gctaaattat ttggtttata accaagagtt
      781 ttcatagctt catgaacttt ttgttttgtt ttttctgata aatatccctt attattaatg
      841 acgcgtgaaa cggttgttgg gctaactcca gctaattcag cgacatcagt taattttgct
      901 accattttaa tattttaatt caaagtattt tccagtaggt tcgcctgatt tgatttgaat
      961 accattttgg tgattttcag ggaatacacg actagtaaac actttttctc ctttattaat
     1021 aaaaatctca atgattgagt tatctacaaa aatgttagca gttgtttctt tagtatcaat
     1081 tgagcattca cgaatcgtac caaagtcagt ggcatattga atgcctgctt tgctgcgatc
     1141 taaaataatt tttccttctt tggtatctac aatcaagcta agtccatttc ctttactatc
     1201 agagaaaaga agtaactccg atttctgatt tggaggaaaa gttagttcaa gctcataagt
     1261 attatttgtt tctgcttttg ctgtaaattg ttcagaacta gcacgtaatg atgtaatagc
     1321 ttctactgga tattggtaaa gtttaccatc ttttattgaa agttctttta caagactcat
     1381 tgctccctga taatcatact tatcagttgg ataatcgata tcaggtagtc caatccaact
     1441 gacaatcaga gcacgaccat caggagtatt gaatccttga gtcgcatacg cttcaaatcc
     1501 ataatcaaga ttttgaattt ctgaagcgcc cacaagtttt actttttcta tgtcaagcga
     1561 ttgacagatc ttaaaagtat tcggatagat attcccataa tttagctcag atttatccaa
     1621 cccttgagga caataaatga gaacaggttt tttgtctaca aatactagat ttggacactc
     1681 aatcatgtat tctgaaccag tgccgccaaa atcaaggttg ccaacttcct cccatgtttc
     1741 cacgttattg tcatccgctt tgtaaagttt gataaagcct gatttttcta aattttgtgc
     1801 tccaacaatc gcatacaatt gtccttcata attaaacatt tgtggatcac gaaaatgatc
     1861 agttacatca gcaggtttat gaataagaac attctcacct ttcgtaatgt tgtagtcttt
     1921 atccatccat gctccgattt gaagtgtatc acgttcccaa ttttcatttc gaacgtttcc
     1981 tgtatagagt aagaatagat tcccctctat ttcatatgca gaaccggaat aagcgccgtg
     2041 gctatctcgt tttgtgtctg gaagaagttc tgctcctgtt acatgaaaat gaactaaatc
     2101 atctgattca ttgtgtaccc attctttcaa cccatgagca gctccaaatg gccaattttg
     2161 ataaaaaagc gtatattttc cgttaaaaaa agaaaaacca ttaggatcgt ttaacaatcc
     2221 tgttttaggt tcgatatgat agctagcacg ccaaggtgat cgttttacat tatccttaat
     2281 tttatccatc tcatctttag tccaatcaga ataagcacga tatcgtattt ctcgaggtaa
     2341 gttcatattt tactccttca atttatcttt ttacaactag tatggtaaac gttttccgtt
     2401 gaaaaatcaa tcatttcatt taaaaaaata aactatatga caaacgtttg acatattttt
     2461 aagatatgct aaaataaatt tgcatttcag tgaaccgttt tcacgtaaaa ataaaaaagg
     2521 agtgattcga atgaattaca gtaaagtagc ttccgacgta atacaagcgg tcggaaaaga
     2581 taaccttatt gcagctgcac actgcgctac acgtcttcgt ctcgtactaa aagatgacac
     2641 gaaagtgaat caaaaagctt tagacgaaaa tccagatgtg aaaggcacat ttaaaattga
     2701 tggacaatat caagtgatta tcggtgcagg tgatgttaat ttcgtttatg acgaacttat
     2761 caaaaaaact ggactttcgg aaatttcaac agatgattta aaacaaattg ctagtaaaga
     2821 taaaaaattc aaccccatta tggcattaat taaattatta tcagatattt ttgttccaat
     2881 tatacctgcc cttgttgcag gtggtttatt aatggcatta caaaactttt taacagctgc
     2941 tggacttttt ggaccaaaat caattgaaga aatgtatcct gcgattgaag gtatttcctc
     3001 aatgatccaa ctgatgtccg ctgcaccatt catgttctta ccaattttag taggtatttc
     3061 agctgctaaa cgttttggag caaaccaatt tcttggtgct gcaattggta tgatcatgac
     3121 aacaccagat cttggtggct caactgaata ttggaatatt tttggttatc atgtttcaca
     3181 aacaaactat gcttatcaag ttattccagt tttagcagct gtttggatat tatctatatt
     3241 agaaaaattc tttcataaaa aattgccatc atctattgac tttacattca cgccattatt
     3301 atcagtaatg attactggat ttttgacttt cacagttatt ggccctgtaa tgttattgtt
     3361 gtctaatgga attacagaca ttattgtttg gctatataac actactggat ttattggaat
     3421 gggtattttt ggaggaacat attcacttat agtcatgact ggtttacatc aatcatttcc
     3481 agctattgaa actcaattac tttcagcgtg gacaaatggt atcggacatg gagactttat
     3541 ctttgtagtt gcatctatgg caaacgttgc tcaaggtgct gcaacatttg ccatttggtt
     3601 cttaactaaa aattctaaga caaaaagtct tgcttcctct gcgggtgtct cagcacttct
     3661 tggaattaca gagcctgcct tgttcggagt taacttaaaa taccgctttc cattcttttg
     3721 tgctttaatt ggttcaggaa ttgctacagc agtagcagga ttacttaaag tagttgctgt
     3781 ttcacttggt tcagcaggtt tcttgggatt tctttcaatt aatgcaacat ctattccatt
     3841 ttacgtattg tgtgaattga ttagctttgt agttgctttt gcaattactt acttctacgg
     3901 taaaacaaaa gctgctggta tctttaaagc agaagaaatt gctgaacaaa caccagttaa
     3961 ttcttcaaaa gtagcaacaa cagatccaat atctactgat gttgaatcaa cacaaacaaa
     4021 agtttcatca gaaacaattg ttagtcccat ttctggtaaa gtgattagtc tttcttcagt
     4081 aaatgatcca gtcttttcat cagaagctat gggaaaaggg attgccatta aaccaaatgg
     4141 aaacacggtc cattctccag tagatggtac tattcaactc acgtttgaaa ctggacatgc
     4201 ttatggtatt aaatcaaata atggtgctga agttctaatt catattggta ttgatactgt
     4261 atcaatggga ggaaaaggat ttacacaaaa agttactact aatcaaaaag ttagaaaagg
     4321 cgatattctt ggagcatttg accgttcaat gattgagaaa gaaggtcttg atgatacaac
     4381 aatgattatt gtgaccaata cttcggatta ttcagaagta aatatagtag ctga
//
LOCUS       DACDHJ010000181         4429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDHJ010000181 DACDHJ010000000
VERSION     DACDHJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.184"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(148..2334)
                     /locus_tag="IV223_002221"
     CDS             complement(148..2334)
                     /locus_tag="IV223_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8712479.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2552..3235
                     /locus_tag="IV223_002222"
     CDS             2552..3235
                     /locus_tag="IV223_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8712480.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3316..4035)
                     /locus_tag="IV223_002223"
     CDS             complement(3316..4035)
                     /locus_tag="IV223_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8712481.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
BASE COUNT         1168 a          873 c          737 g         1651 t
ORIGIN      
        1 gtcacacctc ccttttccga ataaactgtg ttccagaaat agcttcttcg aaaataagct
       61 gaaatttcac aaaaatttga gaagcaattt tcgtacattc cttcttcttt cttgaaactg
      121 accacttctg tcacaccctc tcttctatca ctttcctttt ttcacacgac tcccggtttc
      181 tttgataaat ctcgatactt tcaattttct tgaatttttc atttgaggta cacatagata
      241 cagacgattt cttgctctag tcatcgcaac ataaaatagt cttcgttctt cttctatcgt
      301 cgttactggt tctgctttta tttcattaaa gtcgattact ttcttttctg tcgctccgtt
      361 ataaggcaaa cttccttcgt tacaaccaat caaaaagaca tttcggtaac taagaccttt
      421 acttttgtga atgctctgga tacatagtgc atcaagtttt ccttgtctgt aagcaatcaa
      481 gcgggttttt aattctttag ctgtcgcttg ggtttcttgc gataaggctt ggagtcttct
      541 gaaagtagca tgctgcttta cttcttctcg tatccattct tgatcactct ggtcgatttc
      601 cttagctata cgtggcagat gaagatagct ttgaacaagc gaatagattt cttgatgctg
      661 gtctgtttta cgcaacgctt cctcaaactc tcggatttct gcagatttcg attttaaatt
      721 ggggcttatc gacaaaatat aatctgcaac ggatatattt tgatatttag gagaaaagta
      781 ttgatcgcat tcactttttt tcaggtagca gtctgccatc agctgccgga gcaattgctg
      841 gtaggcctga tttttctttt gcagattcag tgctgtttgc caatgaacaa ttttttgata
      901 gaaatgttgg aaagacttga taccaaagat accttcatat ggactatctg cttccagtac
      961 aaaaggaata ttcgttttca gcagagattg gacaagctgc tgcgtttgtg aagctgatcg
     1021 gtataatatg attgattcct tatatttccc atttttttga ataaatgtct gaatttgttt
     1081 gattagccac tgactttctg ctttagtatc agaaaacaaa taggcttcca taacacctgc
     1141 ttctttttgt gctggaatca tcgcctttgg tatccgatca tttttattat agctgatcaa
     1201 tctatttgct ccttggacaa tagctgcccg agaacgataa ttagttaata agaaaagagt
     1261 tttggtagga tattcttttt caaagttctt gatgtatgct ggctctgatc ctctgaatcc
     1321 atagatactc tgatcatcat ctccaacaac aatcaacttg atcctgtttt cctttgtcat
     1381 ccgtttcacg atttcccatt gaagtggatt agtatcttga aattcatcaa tgaatagata
     1441 atcatatttt tggccgatat aatgctggat ctcctcattc tccaaagcgc ttttcagatt
     1501 ttggagtaca tcattgaact ctagcaattg ctggcttctt ttttgttctt ggagttccgt
     1561 gagcaatcca tacagtcgtt ctgatatcgg ctgtcgtgca ttcttatcaa tggaatcata
     1621 tttttcttta actaactgaa ccatttcttc tactgaaagg ttcatattct gccaatagtc
     1681 taaccgagaa gcaatcgttt tttctccata ttgatcaaat atataaagat tatatttacg
     1741 tatcagctgt tgcaaaacag cattcacttt ttgtgcagta tcctttgaaa agtctccttg
     1801 tattccttgc attgctactt caggaaattt tcgacaaatt tcattgtata tcctaaaaaa
     1861 gatactatgg aatgttccga tatggacagc ggtttttctc tggctgaaat tatctactcg
     1921 tttttttagt tcatttgcag cgttccttgt aaaagtgatc attagtatgc gttcttcgtc
     1981 tacttgtttt tgcgtcatca gataaacagc ttttgcacag attgtttgcg ttttgccgct
     2041 cccagctcct gctatgattc gaagatgttt cttcatatca tatgtgatag ctgctttttg
     2101 ttcttgattg aagcgattat aggaacgttt ctccatcatt ttaaagaaag agctgtttcc
     2161 ttcactttct tttacttgta cttcttcctc aataagtccg ggtgcttcgc cataagccgc
     2221 ttcataggta gcaatgattt cttcttgtag ccgaaagact tgttgaagct ttttatactt
     2281 gttctcccag cgtttcaatt ctttttgtaa tcggagaatt tctttttcat tcaactgatt
     2341 gatttccttc ccatctttct tctttgaatt tgtttatttc aagttggtta tttaaagttt
     2401 cgttgtctat tttttcttta tactaacaga cctttctaaa aatgaaaatg ggaagcgata
     2461 tgattttagt tttctgaaaa tttttccttt ttttcaatcg tttatgctaa gataaggaag
     2521 acaaaaatat tcataaaagg tgatgaaaaa aatgcagact tcctttcacg aggggcgtac
     2581 aataggactt ttgctgacat ttatcggagg agccatggac agttatactt atatccaata
     2641 tggtgctttt gcctctgctc aaactggcaa tatcattcta tcgatcatcc aagcatttga
     2701 cggagaatgg ctaagtgttg gtaaaaaagt attgtctacc cttttctttt tcttagggat
     2761 tcttctaaca aagtatctaa ttgattattt tagaaaaaaa gaaaaacatt tctggcgctt
     2821 gtttgttcta tattatgaag cagtgatttt tttctttgtt agtttgacac ccgtccatga
     2881 gcatcccgca ttagtaacga ttttgattgc ttttactgca tcgatccagt gggtagcttt
     2941 tgataaaatc aacgggcgtg cctacactaa tttatttaca actggaaatt taaaaggggt
     3001 agcaacgaac ttatatgatt attgtatttc taaagaaaaa gcagattttg atcgattttt
     3061 ccactatcta agtgtcgttc ttgcctttat cgcaggagct attgtccttg ttttctgtca
     3121 tcatctgttt ggcgcccatt cgattttgat cgtttccatt ttatttttga tcatggccct
     3181 cactcagaca tttcatttat ggcaatttta tcatacgaat cggttcacta tttgatctca
     3241 atgaatcaaa aaaactaagc aagatgctgc tcacagagaa atatctgcga gtgagcaatt
     3301 tgcttagttt ttatattatt tagttgtaaa gattcccttt gctttcgggt tatttgtagt
     3361 gtagcggatc gactgggcat ctgcctcttc tgtcattttc tttgcccgca tcttgatttc
     3421 ttgtacgatt tgttctccac atgccggatc ggctacgaaa tcatatttgt ccccatcgat
     3481 cactattttg ggacatatat caaactgttc ataccactct tcgtatcgtc gattgagttc
     3541 tttatagtag gaatagagtt caggatcttg ttctagctgt tcatattccc ggcctctttt
     3601 tttgattctc gctaacatag tttcaaagga tacacgaata taaaccagta gatctggttt
     3661 cttttgtggc gaaatttctt ctaactcctt cagcatcgtt tctaaaagac cttcatactg
     3721 ctttacttct acttcagaaa cacgccctaa atctgcattc aaatgaaaca gcatactatc
     3781 ttcgtagatg gagcggtcta gtacgttgtc atcatggctg aaagcatctt tgattgccaa
     3841 aaagcgtttg ttcaaaaaga aaatctgtaa taaaaaagta tacttttctg gattagaata
     3901 aaatagcggc aatacttcat tatcttccac gttctcataa aaaggttttg tttctaaatc
     3961 ttgggcaagc atttcagtta gtgtgctttt ccctgcaccg atcgttcctg caacaatgat
     4021 cacaattatc ggcatccttc ctgtaataaa atctgttctt cattttacta cttcatttat
     4081 agaaataaaa gccttgactt tatctgctcc ttaaaagcta cttctctaaa agtagccaaa
     4141 agtaactcct attcatgaaa ataaaggctg tgacaaaagt cttgtcgcag cccttttttc
     4201 aaataaacag tattctcctg ccaacacctc ggtcacaagg caatattcat aaaacatgaa
     4261 aagctgtttt ttaacattat tccttgtaac tcagagctaa acgggagact cacaacctct
     4321 ttatgctctc aacattttat ctgtttttat tcttctattg tacttgattt gcgtacaact
     4381 aactgccagt gcagattatg agcggtatca ttgtcttcta ctgcgcatt
//
LOCUS       DACDHJ010000182         4426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDHJ010000182 DACDHJ010000000
VERSION     DACDHJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.185"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            32..928
                     /locus_tag="IV223_002224"
     CDS             32..928
                     /locus_tag="IV223_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8712482.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            931..1644
                     /locus_tag="IV223_002225"
     CDS             931..1644
                     /locus_tag="IV223_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8712483.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            1664..2182
                     /gene="msrA"
                     /locus_tag="IV223_002226"
     CDS             1664..2182
                     /gene="msrA"
                     /locus_tag="IV223_002226"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8712484.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            2179..2406
                     /locus_tag="IV223_002227"
     CDS             2179..2406
                     /locus_tag="IV223_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8712485.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            2558..3109
                     /gene="lepB"
                     /locus_tag="IV223_002228"
     CDS             2558..3109
                     /gene="lepB"
                     /locus_tag="IV223_002228"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8712486.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(3192..3734)
                     /locus_tag="IV223_002229"
     CDS             complement(3192..3734)
                     /locus_tag="IV223_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8712487.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(3971..4369)
                     /locus_tag="IV223_002230"
     CDS             complement(3971..4369)
                     /locus_tag="IV223_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8712488.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         1588 a          780 c          799 g         1259 t
ORIGIN      
        1 ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct
       61 cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc
      121 gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga
      181 aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa
      241 gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc
      301 ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt
      361 aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt
      421 tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc
      481 ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg
      541 atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa
      601 ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac
      661 ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg
      721 attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga
      781 aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa
      841 tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca
      901 atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac
      961 aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt
     1021 ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa
     1081 ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa
     1141 aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa
     1201 tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt
     1261 cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta
     1321 aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg
     1381 atcaagagaa actataaatt accagattgg atcgaagtca atacagatga tctgactatc
     1441 atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc
     1501 gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct
     1561 tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa
     1621 aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt
     1681 tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac
     1741 agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag
     1801 ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata
     1861 tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt
     1921 cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat
     1981 cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt
     2041 tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc aagagtatta
     2101 taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga
     2161 aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc
     2221 ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt
     2281 cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat
     2341 ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac
     2401 atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg
     2461 aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa
     2521 agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat
     2581 taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt
     2641 gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa
     2701 gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt
     2761 ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa
     2821 tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct
     2881 aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca
     2941 agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg
     3001 aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa
     3061 agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa
     3121 gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt
     3181 agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat
     3241 gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga
     3301 aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg
     3361 ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta
     3421 tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct
     3481 tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa
     3541 ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg
     3601 atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa
     3661 agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca
     3721 atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat
     3781 caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa
     3841 gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga
     3901 ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt
     3961 agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga
     4021 cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac
     4081 cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca acactaaacg
     4141 aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt ataaattttt
     4201 tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag
     4261 cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg
     4321 aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct
     4381 cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgt
//
LOCUS       DACDHJ010000183         4420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDHJ010000183 DACDHJ010000000
VERSION     DACDHJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.186"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(42..653)
                     /locus_tag="IV223_002231"
     CDS             complement(42..653)
                     /locus_tag="IV223_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8712489.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(776..901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(983..1699)
                     /locus_tag="IV223_002232"
     CDS             complement(983..1699)
                     /locus_tag="IV223_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8712490.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1705..2274)
                     /gene="scpB"
                     /locus_tag="IV223_002233"
     CDS             complement(1705..2274)
                     /gene="scpB"
                     /locus_tag="IV223_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8712491.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2258..3055)
                     /locus_tag="IV223_002234"
     CDS             complement(2258..3055)
                     /locus_tag="IV223_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8712492.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3025..3396)
                     /locus_tag="IV223_002235"
     CDS             complement(3025..3396)
                     /locus_tag="IV223_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8712493.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3415..4302)
                     /gene="xerD"
                     /locus_tag="IV223_002236"
     CDS             complement(3415..4302)
                     /gene="xerD"
                     /locus_tag="IV223_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8712494.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1200 a          969 c          774 g         1477 t
ORIGIN      
        1 ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga
       61 cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact
      121 aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt
      181 acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag
      241 taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt
      301 cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa
      361 taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa
      421 ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca
      481 actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg
      541 atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg
      601 atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat
      661 cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact
      721 ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta
      781 tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg
      841 caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa
      901 ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct
      961 aacaattata aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt
     1021 tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt
     1081 cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat
     1141 tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata
     1201 aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag
     1261 gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt
     1321 catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc
     1381 ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa
     1441 gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt
     1501 tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag
     1561 tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac
     1621 gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta
     1681 ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa agaatagatc
     1741 tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa
     1801 tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc
     1861 tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc
     1921 tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata
     1981 agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc
     2041 atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt
     2101 ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac
     2161 taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat
     2221 cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg
     2281 tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga
     2341 tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag
     2401 aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt
     2461 cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt
     2521 tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt
     2581 tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt
     2641 cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt
     2701 tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata
     2761 attcttcatc gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca
     2821 tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg
     2881 tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga
     2941 tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt
     3001 taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc
     3061 aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa
     3121 ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc
     3181 atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt
     3241 tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat
     3301 ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc
     3361 aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc
     3421 ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat
     3481 ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc
     3541 attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta
     3601 aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc
     3661 tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg
     3721 tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg
     3781 ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa
     3841 tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc
     3901 acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc
     3961 tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat
     4021 cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga
     4081 gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg
     4141 gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg
     4201 caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc
     4261 aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc
     4321 tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga
     4381 aaaagccgtc tagacactca gacggactgg tttctttttt
//
LOCUS       DACDHJ010000184         4339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDHJ010000184 DACDHJ010000000
VERSION     DACDHJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.187"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..947)
                     /locus_tag="IV223_002237"
     CDS             complement(51..947)
                     /locus_tag="IV223_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8712495.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(940..1620)
                     /locus_tag="IV223_002238"
     CDS             complement(940..1620)
                     /locus_tag="IV223_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8712496.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1621..2403)
                     /locus_tag="IV223_002239"
     CDS             complement(1621..2403)
                     /locus_tag="IV223_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8712497.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2396..3313)
                     /locus_tag="IV223_002240"
     CDS             complement(2396..3313)
                     /locus_tag="IV223_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8712498.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3477..3800)
                     /locus_tag="IV223_002241"
     CDS             complement(3477..3800)
                     /locus_tag="IV223_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8712499.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1275 a          946 c          757 g         1361 t
ORIGIN      
        1 tatatataag ataatacatt tttctactag ttctcactaa tcttgtataa ttaaaccctc
       61 cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat ctctcctccc
      121 attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc cgtatttgtg
      181 cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt ttgcgttcct
      241 ggtttgatag gattggccac tgttaatcga atgaatggat cctcttttga aaatgtcacc
      301 tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag cactcgcagc
      361 aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa cgctacctcg
      421 atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac ctctttagaa
      481 aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc aagatcataa
      541 aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt taaatacttc
      601 tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt aatagcagtc
      661 aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt ttcttccatt
      721 tcttgaaatg cttgtccttt ttgttcattt tcatccaacc atgtattcag tgcatctact
      781 aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata ttcttgtcgt
      841 tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag gaagaaaata
      901 aaacttccca taaagataac aacgagaagc aacagccatt taatcaaatc taaaccccac
      961 tccccacacc gtatgaatat acacagcatg tgcctttgct agcttccctc gaagattgga
     1021 aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa ctgtttcata
     1081 aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca gcagatcgaa
     1141 ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg actctttgtc
     1201 cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga cagaagtctg
     1261 atgacgaagt tggatgccga ttcgtaacaa aagttcttcg atatcaaatg gtttcgtcac
     1321 atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc tttttgctga
     1381 aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg cttcacctga
     1441 aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc ttaacaataa
     1501 ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa gaacctcctc
     1561 cagtaaggaa tgaacagctt catcgtcttc gataatcaaa atagtttcca gcaggaccac
     1621 ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata ccacgattgt
     1681 tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt gcgcaaagtc
     1741 cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc ctttgatttt
     1801 tgcgaaaaca ccgggaatga aggccgttcc catgatataa agaaaagaaa gaagcaaact
     1861 gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg tccctaatgt
     1921 cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt tgattccctc
     1981 aacttcacca aatccgaaag caagtgtgta agcaagcatg gctagtcccg tgatcaaaac
     2041 cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat aagtcattcg
     2101 attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt ccccaatata
     2161 aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt ctaccattaa
     2221 aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat tgccgtattt
     2281 tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca tcagtcctag
     2341 tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta atagattaaa
     2401 catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc ccaactaggt
     2461 gaataccatc cacaggaact tttgcttcta ccaatgcttg attgatggct gctacttggt
     2521 caattccttc aaaaagctgg ataacacggc tagattgtac aaaataatcc ctataaccaa
     2581 gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt ataaattgac
     2641 gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct tcatgcaaaa
     2701 taccatagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa agcaagacgg
     2761 tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc atccctgctg
     2821 gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg atcagtgcat
     2881 ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc tttttccctg
     2941 tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac acaccgccgt
     3001 tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga gcaggggttt
     3061 caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca aataattcga
     3121 tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta gacttacccg
     3181 ctccattttc cccaatcaat ccatagatca ttccacgttt gatttcgatt gatactttat
     3241 ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag accgtttcat
     3301 ttccaaaatc catacttatc cttctttctt attgatgagt gaagtatagt tgacgggtat
     3361 aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa tgtcaaaaaa
     3421 aacaatcgga caatacgtgt ctttcctatt atcaggcagt ttgttatacg cttgtatcag
     3481 tttcctgatt ctttcttttc gaccaaagtc gtttctctgt attcctcttc actgaaaatt
     3541 tttgcttctt tcagatcaag cttgagtaat tttaccagac cggttataag gataactata
     3601 gcaagactcc ctcccagaaa aattagactg tctcgcagac gtggttgcgt tgtcttccaa
     3661 tcaaagagcg caccaaacag tgctaatcca agtggactgg ccaccatggt aatcgttgtt
     3721 atcgttgaca taagtcgtcc ttgtatatgc gttggaactt ctcgctgaag ccagaccata
     3781 taaaaagcaa aagaaaacat taatccttaa aaacggactt atgaaaaata atacatgccg
     3841 tttgggattt acaattagct accaaataag aggttatgac aaaagacttt tcacaatccc
     3901 ttcttccgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatttatc
     3961 aaacatgaga agcaattttt gtaaattcct tcttatttct tgaagctgac cacttctgtc
     4021 acaacctcag aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt
     4081 ttatagatcg aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca
     4141 aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc
     4201 tcagcattgc ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat
     4261 taacgttttg agaactgtga agctttacgg gctttcttaa gacctggttt tttcttcgta
     4321 tcattgaata agtttgaaa
//
LOCUS       DACDHJ010000185         4309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDHJ010000185 DACDHJ010000000
VERSION     DACDHJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.188"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..446
                     /locus_tag="IV223_002242"
     CDS             24..446
                     /locus_tag="IV223_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712500.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            456..659
                     /locus_tag="IV223_002243"
     CDS             456..659
                     /locus_tag="IV223_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712501.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            870..1454
                     /locus_tag="IV223_002244"
     CDS             870..1454
                     /locus_tag="IV223_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8712502.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1825..3150
                     /locus_tag="IV223_002245"
     CDS             1825..3150
                     /locus_tag="IV223_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8712503.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3143..3493
                     /locus_tag="IV223_002246"
     CDS             3143..3493
                     /locus_tag="IV223_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712504.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3490..3669
                     /locus_tag="IV223_002247"
     CDS             3490..3669
                     /locus_tag="IV223_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712505.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            3805..4095
                     /locus_tag="IV223_002248"
     CDS             3805..4095
                     /locus_tag="IV223_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8712506.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         1532 a          631 c          819 g         1327 t
ORIGIN      
        1 ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta ggtattttaa
       61 ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt acaaatgaac
      121 caactactgt agtagataat aaccaagaat taactccttt agataggaac ggaatagcca
      181 ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac
      241 ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg
      301 gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt
      361 actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt
      421 attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa
      481 atttttatca agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt
      541 tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag
      601 aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat
      661 gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa
      721 atcacccatt tctggacact tccttttttt gctttatttt tcaaaaaaac aattcatttc
      781 atgttcataa acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt
      841 acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg
      901 gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt
      961 ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt
     1021 ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg
     1081 aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc
     1141 aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg
     1201 cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag
     1261 ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg
     1321 aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga
     1381 acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga
     1441 caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag
     1501 tgacaatagt atgagaattt tttgtaagtg tctactgata acttattatc aaatgttgta
     1561 aggattttat tcatttgtta atattaaaga acggaactcc aaaaaagtcc gtactctaat
     1621 ttgcagtctc tcactctttt agagtgagag tttttattag attttcaaat taattagtaa
     1681 agtgatagaa taaattgatc atacaaatca taagaagaag cagggtgata gcatacctat
     1741 gcttcttttt ttatgtattt agctatttct tctttacaac atcgaacata tgtttgtata
     1801 atgtggaaga atggagtgtt atttatgatg gattattcaa aagagccagt aaatgattac
     1861 tttttaatcg atatgaaaag cttttacgca agtgtcgaat gtatagaaag aaatttagat
     1921 ccattaacaa cagaacttgt tgttatgagt cgatctgata atactggttc aggattgata
     1981 ttagcttctt ctcctgaagc aaaaaagcgg tatggtatta caaatgtgag tagaccacgt
     2041 gacttaccac aaccatttcc taaaacacta cacgttgttc caccacgtat gaacctatat
     2101 atcaagcgaa atatgcaggt aaataatatt ttcagaagat atgtggctga tgaagatcta
     2161 ctgatttact cgatcgatga atcgatttta aaagtgacaa aatcactgaa tctttttacg
     2221 actgaagaaa cacgaagtca gcgaaggaag aagctcgctc aaatgatcca agaacgtatt
     2281 aaagaagagt taggtttgat tgctacggta ggcgtcggcg ataatcccct tttggctaaa
     2341 ttagccttag acaatgaagc aaagcataat gaaggattta ttgcggaatg gacgtacgaa
     2401 aacgttcctg agaaagtatg gaatattccc gaaatgacgg acttttgggg aattggttct
     2461 cgaatgaaaa aaaggctcaa ccaaatggga attttgagca ttagagacct tgctaactgg
     2521 aatccttata cgattaagaa tagattaggt gtgattggat tacagctgta cttccatgct
     2581 aacggaatcg atcgaacaga tatagcaatc ccgccagaac cgacaaagga aaaatcttat
     2641 ggtaattcac aagtgttacc acgagattat actagaagaa atgaaatcga gctggtggta
     2701 aaagaaatgg cggaacaagt agccattcgt atcaggcagc ataattgtaa aacaggatgt
     2761 gttcatttaa acattggtac gtcgatctta gaaacgagac caggattttc acaccagatg
     2821 aaaataccta tcacagataa tacaaaggaa ttgcagaatt attgtctttt cttgtttgat
     2881 aagtattatg aaggacaaga agtaaggcac gttggaataa cctatagcaa gcttgtgtac
     2941 accgattcgc ttcagctgga tttatttagt gatccacaaa agcaaattaa cgaagaaaat
     3001 ttagataaaa ttattgacaa gattcggcaa aaatatggtt ttacgtcgat tgtacatgct
     3061 tcttcaatgt tagaaagcgc tagaagcatt actagaagca cacttgttgg tggtcatgcg
     3121 ggcggaaatg ggggaattaa aaatgattga ttacaatgat cgtggacgtt taaagtgggc
     3181 agggttttat ttatctgatc atactgaaga aattgatgcc gacaatgaac aacgaaccag
     3241 tcaaaattta gcaaaagaac aaatgacgac tgaagagatt gcggaagtat tgaacgctgc
     3301 agtcataaaa aatagatcag tatctattca aaaagaagaa cgaaacgctg aaggatacta
     3361 tccagaagat attgttggca agatcttagg tagtgatgag ctggggatct acgttggaaa
     3421 agacaaaatt cactacgacg aaatacgtca tatcgaattt taccaggata ataaatggag
     3481 tcgattagaa tgaacattat ccagcaatat gagctaaagt atataacctt tgatcagcta
     3541 tcagaagaga tttggggtta tggccaacgt ttgataaatg aggttggtgt tgagcgcttc
     3601 tctttctatg tagaagctgc agcaggttat cataatttta ggttttatat tttcccgtta
     3661 tatatataag gtattttacg gattctatag agttatctct atgaatcctt ttttgttttt
     3721 acaaatagga gtagatacta gaataatgcg tgtgttaaac tattattgca aacgcaataa
     3781 taaaagaaag taggcttacg caccatgtct atcaatacat ttgtttactc gcacccgatc
     3841 aatgtttaca ttatcaagaa tttaggaatc actgtagaac aattttgtga gttatatgcc
     3901 tacccacaag gaacggttgc aagctggata acccgacaac gtagaataaa atcattacct
     3961 gcttcattcg tttatgattt aagcctggcc tctagtttaa acatgtctga tgtttatgaa
     4021 aagctcctgt ctttagaaaa agaatatgat tccttcaaga ttaaacatga taaaaaaata
     4081 aaaaaacata tataatatca atcacaattg tataatatta cgatatctta atatcatgat
     4141 atcgtaatat tgtttttttc taagaaaatg cactataaga agggaattat tagtaacaat
     4201 atagacagtt taataatgtt taaattattc tagttgctgc tcgcaaaaca tatttcttat
     4261 gattaataaa aaattttaaa gtaataaaaa gtgattattt tactttcga
//
LOCUS       DACDHJ010000186         4233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDHJ010000186 DACDHJ010000000
VERSION     DACDHJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.189"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            335..1066
                     /locus_tag="IV223_002249"
     CDS             335..1066
                     /locus_tag="IV223_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8712507.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1211..3238
                     /locus_tag="IV223_002250"
     CDS             1211..3238
                     /locus_tag="IV223_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8712508.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3346..>4233
                     /locus_tag="IV223_002251"
     CDS             3346..>4233
                     /locus_tag="IV223_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8712509.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKG"
BASE COUNT         1481 a          695 c          880 g         1177 t
ORIGIN      
        1 gataatcaca aaaaaaaata taatatacat tttttattta attataatta atattgattg
       61 aagtctaggc agtcaagtca cattttgata gataggaggg ggacttacga attattttaa
      121 aggctatgac agctgtattt ctagattgtg tttgcatcaa gttgcaataa aaactagaaa
      181 tcaaatcggg ctgataatca tttataacta atcgatacat aggaccaaat ataggtctat
      241 gttcttttat ttcataagta tcgtaaaaca gaattttgct atggattaat ccaatcgact
      301 gatatgttta gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac
      361 agcagcaatg atgttattca gcatggtagc aagtacgact tttgtttctg caactgaagt
      421 atacccaaag gaatataaca cagaaggaac gattacattt gaagcaggag atgagggtgt
      481 aactcctcct gttgatccag agaatcctga tcccaataaa cctgtcgatc catcagatcc
      541 tccaagccct ggaacaggag gggcattatc gattgattat ggatcaaaat tcaaatttgg
      601 gactcaaaaa atttctactg cggataaaac gtattatgca gctgctgatg tgatgaacga
      661 tgggtctaga aaaccgacat atgtccaagt gacagatagg agaagtacct tatctgggtg
      721 gaaattaagt gtctctcaac cagaacagtt taaaactgcc agtggggatg aactagttgg
      781 agcacagctg aaatttacga aagggcaggc tgtttctttg gttgatccaa catatacgcc
      841 gcaaacggtg aattctgaat taacgttgac accgggagga aacaatacgt tagcaattaa
      901 tgctaaatca ggaacaggtg tggggacttg ggtctatcga tttggtgcaa atgaaaatga
      961 aaatcaagat gcggtacagc tttcggtacc cggtaaatct gtcaagttag cgcaacaata
     1021 ttccactaaa ttagtttgga cattagaaga tacacctaac aactagttta ctgaaaagaa
     1081 ctgttaaaaa taaatacgta gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa
     1141 aggctaggct aaatttgttt ggatacgaat agttcatcta ggaatttaac gatcaggagg
     1201 aaaagatata ttgaaaaaga ggaggatcgt gctcatttta agcagtctct tagtgactaa
     1261 tatctttccg atcatagggt actctttgga attgaatcag ttggagcatg gaaatagaag
     1321 agacgaagaa agcaaactca cttcgacatt cgacagttta atagaatcca accagacaca
     1381 agaaagcagt gaggatttaa gtatttcgag tagtggagag accactgaca acgtagaagc
     1441 tgagggttcg gacaaacaag gactagaaga tgcaccaatt tctgcggttg agctgcctga
     1501 cgatcccaat attatttcta tcgataaaat ttttcaaaca cctatcggta ctagcacgtc
     1561 catattggaa aatgggaagt tactgcagtt gaacccagca gaaaggtctc aaagaggagc
     1621 tatttggtca aataagaaga ttagtttact ctcggatttt acatttaaat cttatttata
     1681 cttaggaaat caacgagata gagcgggaga tggcatgacc tttacattga ctggcgatcc
     1741 tagaatggct agtaatccca gtcaagttat tggatcgcca ggtatgggaa ttggctcata
     1801 ttctactcgt tctggccaac cttacattgc gaatgcgctc tcgattgagt ttgatacgta
     1861 taaaaatagt ggttcttctg atcgaatgga tcgagagata actgctgata atagagggca
     1921 tggacatgtc gcttttgtca cgcctaaacc aaataataat aattattctg gagagcatac
     1981 tggcgtgacg gtagctccta catttctttc taatggcaca tggcgcttgt taataattaa
     2041 atgggacgcg gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc gctcttctcg
     2101 gattatacct gatctcatta ggcagtttgg ttctacagaa gtaaattggg ggtttacttc
     2161 ttcaacaggt actttttttc aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt
     2221 cacatcaaca gctgatgtaa aagtcaacga tagtgattat ggggccatcg ctgaagctgg
     2281 aggaaacgat aagataacta tcagaaatac attgaaggtc gagggggatt ttcttgaata
     2341 taagaaaatg cagactagta tcgacctccc acaagaatta actattcccg agcatatctt
     2401 gatcaatgga aatcaaattg atgccaaaga tatcttgata accggaaatc gagtcacgct
     2461 aaatctgaca gattatttta ttgctggaga tacgttaata atcgaattgg agacaacttt
     2521 gcaggaagta attcctgaaa aagtactcac tatgcatttt gaataccttg aaaacgggaa
     2581 tttgattgag caatcgaatg aaataaacct aaagatcgca aagctgaagg agatagatat
     2641 ccatgtttat tataaagata aagacagtca gattgatatt gctgaaatga aaacaatcac
     2701 tgggaaaata ggagagaagt ataaagaaac tcctattgat attgaagggt atgtttttga
     2761 ctcggattca gcgaatacag aaggcatctt ctccgaaaat acagaggata tctattttta
     2821 ttacagaaaa ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca
     2881 taaaatatcc agtagaccat taagtgaatt cggacagttt tcaggtgggt tgaaaattat
     2941 agatgaacgg gcttttggaa gttggaaatt acaattgaaa caagagaaag ttcttacaaa
     3001 tggagatttt gagcttccag aagctctttc ctttgttacc tctgaaaata caacggttat
     3061 agggacatcc gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc
     3121 tcttttagat tctacagctc atcgtggaat cagaataaat attcctgtag aaaaccaaag
     3181 aatcggaacc tttgagggga aactatcttg gatacttgca gatgtcccag gaaattaaca
     3241 ggaagagaag aatataacaa aattattgag tttcaagaag ggcacaggta ccataggctc
     3301 ttgtgtccca cagagacaag attttgttat agtcttctct tacatatgat taaaggagcg
     3361 attttcttga gaccaaaaca acaattattt ataacactcc tagttatttt aggggcactt
     3421 tttaacaata tatcagtgtc agctagtgca tttaattttt cagtgacgcc gataacttca
     3481 gagaatcaaa tcgacaagag aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa
     3541 gtagaagtaa aagcggaatt gagaaatgat acagaaaaag aagtgaaaat cgatatttct
     3601 gtgaatagcg caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa
     3661 aaagacatga gcctgatctt tgatctggta gattatgtga gctatccaca gatagtgacg
     3721 ctaaaaccta aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa tgagcgattt
     3781 gatggtgtat tggctggtgg gatcacattc caagaaatcc agacggaaaa ggaccaaaca
     3841 gaaactaagg atcaaagctt atcgattgag aatgaataca cctatattat tgctttgttg
     3901 atgcaacaga cactgaacga agttgctccg aaccttactc ttcatgaagt taaaccagat
     3961 caaatcaatg caagaaatgt catcttagca aacgttcaaa atgatcaaaa aacctatatc
     4021 aatcaagtag ttattgaaac aaaaattaca aaaaaaggac attctgaagt tttatatcaa
     4081 gaggaaaaag aaggattgca gattgcgccg aatacaaatt tttcttttcc aactgcttta
     4141 aatggtcaac cgctaacccc aggtgaatat caccttacaa tgactgtttt aggaaatgaa
     4201 aatgaaaatg gaaaattttc gagaaaaaaa gga
//
LOCUS       DACDHJ010000187         4164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDHJ010000187 DACDHJ010000000
VERSION     DACDHJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.190"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            430..1398
                     /locus_tag="IV223_002252"
     CDS             430..1398
                     /locus_tag="IV223_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8712510.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1409..2224
                     /locus_tag="IV223_002253"
     CDS             1409..2224
                     /locus_tag="IV223_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8712511.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2292..3002
                     /locus_tag="IV223_002254"
     CDS             2292..3002
                     /locus_tag="IV223_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8712512.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3038..>4164
                     /locus_tag="IV223_002255"
     CDS             3038..>4164
                     /locus_tag="IV223_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8712513.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1393 a          629 c          885 g         1257 t
ORIGIN      
        1 ccataaattg cgtactaaaa gaaattctgc aattcaaaat aaagcatttt aagccatttt
       61 caaatttaag tgcacccatc tacccgtcaa atcactttat tttccactcc agtagtttcc
      121 taattcaact gtgtgcaaaa cgatttcttc taaacaaatc aagacaaact tataaacctt
      181 catcccagca ccataattat ttaaatcata ggcatttttc gaaattagta tatgtactca
      241 agtttcactg cagattattt atatttttta gagaaataaa tttttcctat tgacaaacac
      301 aaagaaagcg gatacaatac aattatgtca catgacgtta tatgacgtta tgtaatatct
      361 ttacatttaa tcagttactt ttattttgga taaaattaac atttttaaaa ttttaaggag
      421 gaaatgttga tggatgtagc aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag
      481 atttctggaa tttatttggt ggcttgtggc ggttcgctgg tagatatgta tgtctcagat
      541 tattttctta aagccgaagc atcgaagtgt attacaggct tatatacagc gaacgaattt
      601 aaccatgtaa ccccaaagca attagatgat aaatctgttg tcattctatg ttctcatgga
      661 ggaaatacag cagaaacggt aaaagcagga gaagtcgctc aagctagagg agctgtgact
      721 gttggtctga ctcataataa aactgcggat ttattaaagg tttctgatca tagtttcctt
      781 tacgaatggg gagatgatgc aaaaatagtc aataatccta tggccatcat tcttgattta
      841 actgtatctt tggtcaatgt taccgaaaca tttgaacatt acggtaaatt cagagaaggc
      901 atgaaggtta tcgataccgt aattgaaaat ggcattaagc aggtgcagga tcgtattaaa
      961 gtgtttgctg ataagtacaa agatgaaaca atgttttata tcttaggtag tggaccatca
     1021 tatggacatg cttatggttt ttcaatttgc tctttgatgg aaatgcaatg gcttgattca
     1081 acttctgttc attctggaga attcttccat ggaccttttg aagtaacaga caaagatacg
     1141 aattttattc tcttgatgag cattggacgc actcgtgctt tagatgaacg ggcaaaacga
     1201 ttccttgata aatatgcaga aagagtagaa atcgttgatg caaaggaact aggattggat
     1261 atgatatcag atgaagtttc tgaattcttc aatcccattt tattctacag tattctttca
     1321 gaatatcgtt cagctttagc agatgcacgc aatcatgatt tagatgttcg tcgctatatg
     1381 gggaaagtgg agtactagga ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt
     1441 cgataaatat gaaaatctgg gaattatgta tcctggagga aattcattga attttgcagt
     1501 ttttgcaaaa aaactgggac atgaaagtag ctttatggga gtttttggct cagatgagga
     1561 ggcagctcat gttttatcag caataaaaaa aataggactg gacaacagtc actctcgctt
     1621 tgaaaaaggc gagaatggct gtgccagagt gaagattgat gagggcgacc ggattttcct
     1681 tggcagcaat tcgggaggtg ttactcgtga atacccgatt cagcttactg aagaagacag
     1741 agaatattta aaccagtttg aattgattca tatgggatta tatagccatg tcaatcatct
     1801 attagaagag ttacaaaatg tttctggaaa gatttcttat gatttttctg acgactttac
     1861 ggaagaagag gttcagcggg caattgataa agttgatttt ggcttctttt ctatagaaga
     1921 cttttccgat gaagaagtaa agcagttttt gatgaagcat ttttgtgagc gaaatgatgt
     1981 attgattgca acccgaggag aaaaaacggc tattggttat gatggagcaa agttttatga
     2041 agtgaacccc gtgctagaag aaccagtcga tacaatggca gcgggagatt ctttcttaac
     2101 agctttccta ctttattttc tcgaagggga cgatatagtc accgcgatga aaaaggggaa
     2161 tgaatttgct gggaagtctt gtctggttga cggttcgttc ggctttggta ttcattacga
     2221 ttgagcttgt tttaaattcc tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg
     2281 aggttaaaaa aatgctggat acaaatgcac aattaccatt gtacttacaa ttaaaagaaa
     2341 tattaaaaaa taaaatcaat acaggggagt atcccgagaa tacgaaaatt cccgctgaat
     2401 cagagctgat agaaagttat gaggtcagcc gaatcacagt gagaaaagca gtagaagaac
     2461 tggtagaaga agggtaccta cttaaaaaac aagggaaagg aacttttgtc aaccggcata
     2521 aagtttttcg taaaattgaa tatgttatgg gtttcagtga gagttgtaaa tctaatggtt
     2581 ttacaccgac tagcattgtt ttggaacgca agatcattcc agcaactgaa gggatggcaa
     2641 aaaaactcga tatcgctcaa ggtgatgatg ttttatatat ccagagaaag cgaatggcgg
     2701 ataacatccc cattatgtta gaaaacaatt atttttctta tgaaagatat agtttcttaa
     2761 tggaggaaga gctgactgat tctttgtatg aagtacttaa agtccaaggt accttagcca
     2821 tcaatcctgg ggaaactgca cttgaaattg tcacagctga tgaccaaatt gccaaaataa
     2881 tggaagtagc tgttgggacg ccctttttct ttatggatac tttaatcaac gatcaaaaca
     2941 acataccaat tcatgtaggt aatcaatatt acctgggaga agtatatcgt ttttacttat
     3001 gatttcctgt tttgatttca aggagtagat tttatttatg aagcaaatat tgcggtcaaa
     3061 aatgattttt gatggagagc agccaccttt caaaggttat attgtaatca atgaagggat
     3121 cattgaaaag gtggaaaaca actggaatta cagtgatcta ttaaatgaaa acacggaatt
     3181 aattgcttat gatgatcaat tcgttatgcc agcaattcat gataatcatg tatttttcag
     3241 tggttatttg gctatgaatg ctggcataga tttaagcgat acatcttcag ctaacgaggc
     3301 tgtagagcgt attaaagctg taatgaacca taaaaaagca gatgaaccaa tttatgctca
     3361 tggctgggat gctgccctgt ggaaaactga cccatcagaa gagctattga atgaaataaa
     3421 ccactttggt ccaatcacag cgatcgacaa aaatcgcagc cattgttgga tgaatgctat
     3481 tgctaagcag acttatggtt ttacagaaaa agaaaccagt gctgaaagtc gagtattatt
     3541 aattaaggaa atgctagcaa acaaaaagct tttagcagag gtttacgctg aatttgaagc
     3601 attactgctt tcaaaaggtg ttatatcaat gaaagaaatc gtttttgatg acgcagaatt
     3661 tctttctaat atacctcaga ggaaaatact tagtaaccta tatgtgcagg cagtccaaga
     3721 acctctacag catgaacttc tatatgagta taaggaaaaa gttttccctg aaaaggtccg
     3781 ctttggtgga gtgaagatca tggtagatgg tgttgttgcg gatagaagtg gagatattta
     3841 cggtgtttat cagtcaggag tagcttcacc cgagattgac tatgaggcaa ttaaagcaga
     3901 agttgctgca ttcaatgaag tagagattcc ttgctgtttg acaacggaag gagataaagc
     3961 gggttataaa gctgcgtcaa ttcttgtcga atttggaaag cggttacctg aaggcgtttt
     4021 taattcgatt agtgatttgg aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg
     4081 acaagtagta gcagaggtat atccacaaat tctcggcttg aatcaaacgt tagaagaggc
     4141 ttacatgtca actgttataa aaaa
//
LOCUS       DACDHJ010000188         4159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDHJ010000188 DACDHJ010000000
VERSION     DACDHJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.191"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..292)
                     /locus_tag="IV223_002256"
     CDS             complement(<1..292)
                     /locus_tag="IV223_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712514.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYY"
     gene            complement(500..1426)
                     /gene="glsA"
                     /locus_tag="IV223_002257"
     CDS             complement(500..1426)
                     /gene="glsA"
                     /locus_tag="IV223_002257"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8712515.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1449..2876)
                     /locus_tag="IV223_002258"
     CDS             complement(1449..2876)
                     /locus_tag="IV223_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8712516.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2907..4121)
                     /locus_tag="IV223_002259"
     CDS             complement(2907..4121)
                     /locus_tag="IV223_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8712517.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1307 a          898 c          746 g         1208 t
ORIGIN      
        1 aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag
       61 aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt
      121 ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag
      181 tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt
      241 cacacagata cacttttcta aatatatttc gctcgatgta attttccagc attcctgata
      301 cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt
      361 cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc
      421 aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc
      481 cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt
      541 tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac
      601 ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag
      661 gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa
      721 ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa
      781 ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg
      841 agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac
      901 ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga
      961 tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc
     1021 gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg
     1081 cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat
     1141 tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt
     1201 ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat
     1261 cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt
     1321 gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat
     1381 agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct
     1441 ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga
     1501 ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca
     1561 gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga
     1621 cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca
     1681 agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc
     1741 aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg
     1801 cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga
     1861 gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct
     1921 gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct
     1981 aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt
     2041 aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact
     2101 gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca
     2161 aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg actggcagat
     2221 gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc
     2281 gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg
     2341 acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa
     2401 ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc
     2461 cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata
     2521 cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt
     2581 atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg
     2641 acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac
     2701 aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag
     2761 cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc
     2821 aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat
     2881 cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc
     2941 aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt
     3001 aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga
     3061 atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc
     3121 cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca
     3181 tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg
     3241 atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc
     3301 aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt
     3361 gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca
     3421 ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac
     3481 caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct
     3541 aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc
     3601 gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca
     3661 ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata
     3721 atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc
     3781 acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac
     3841 atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca
     3901 ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt
     3961 atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat
     4021 ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc
     4081 atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta
     4141 cgattacgag tataaaaga
//
LOCUS       DACDHJ010000189         4145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDHJ010000189 DACDHJ010000000
VERSION     DACDHJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.192"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..714
                     /locus_tag="IV223_002260"
     CDS             13..714
                     /locus_tag="IV223_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8712518.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1286..2302)
                     /gene="tsaD"
                     /locus_tag="IV223_002261"
     CDS             complement(1286..2302)
                     /gene="tsaD"
                     /locus_tag="IV223_002261"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8712519.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2299..2763)
                     /gene="rimI"
                     /locus_tag="IV223_002262"
     CDS             complement(2299..2763)
                     /gene="rimI"
                     /locus_tag="IV223_002262"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8712520.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2770..3312)
                     /gene="rimI"
                     /locus_tag="IV223_002263"
     CDS             complement(2770..3312)
                     /gene="rimI"
                     /locus_tag="IV223_002263"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8712521.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3296..4120)
                     /gene="tsaB"
                     /locus_tag="IV223_002264"
     CDS             complement(3296..4120)
                     /gene="tsaB"
                     /locus_tag="IV223_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8712522.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1215 a          887 c          722 g         1321 t
ORIGIN      
        1 gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact cagatatcaa
       61 cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca tactgttctt
      121 cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca acggttgtat
      181 atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac ttattactac
      241 tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg agaagagacc
      301 tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc tgtttcctac
      361 tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca aaaaatttct
      421 aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag cgcctttgac
      481 cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac aaaagatggt
      541 catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag cggcacgaca
      601 cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccggactgtt agattatgac
      661 aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga ataacgcgaa
      721 caaaaataag cagtgaccct gatatttatc agggtcactg cttattttcc tccctaaaac
      781 ttctgaaaga tccggcagtt ttagatcact gggcaagact caaagatttt ctatgtgtgt
      841 ttcagttatg taatattcac tgtttgcaaa aaatactgat agattaacta gaggaagcgt
      901 cttgtcctca tccgacctag attattcgat attcctcgac aataaacgat taatcagtga
      961 gggcaatcag aggaacggat aatgatgaat aagctgtaaa aaatagggct ttgacaaaag
     1021 cgtgtcacag cccttatttc gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt
     1081 cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga atcagagtta
     1141 aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata cgcggtaata
     1201 agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt attacttgat
     1261 gcagggcaga tttttcacaa cctctttatt cagataaaac caagcttgga tcagcattca
     1321 actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct ccaatcattg
     1381 cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca gggagtttag
     1441 ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca cctccagcta
     1501 caatcagttg cttgactgga taattttgac aagctcgaag tgttttagta atcaatacat
     1561 cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct tctccacgct
     1621 gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa ctgaaatcat
     1681 aattatcttc atggatcatc gctcgtggaa aatggtaatt atcttttccc tgatgagcta
     1741 gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca actttgtcat
     1801 aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat ccatcttctt
     1861 gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat ggaaactgga
     1921 atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca ggaataagcg
     1981 gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc aacgagccga
     2041 caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac acaccagctt
     2101 ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg ctagctactt
     2161 caggaactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg atgttagata
     2221 agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt gattcaatcg
     2281 ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc tcataatgat
     2341 cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac gaaacttttc
     2401 tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa aaacatgcag
     2461 cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc
     2521 ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta ctttcgagta
     2581 tccaataaag ccacagatct ggttatcttc aactaaaagc agataatcag agtgaggctg
     2641 ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat aagcattttc
     2701 gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt ttctgcaaat
     2761 caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt cttttgtttc
     2821 agtgtaatat ctttttttga tcgttgttgc ttgaaaacct aacttccgat aaagtctttg
     2881 tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat tcattatagc
     2941 aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat atgccggtcg
     3001 aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga taaaaccaat
     3061 cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact tcaattctga
     3121 tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt cgacatccag
     3181 cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat cgatttgttc
     3241 gattgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat acgctctaaa
     3301 ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt tcttctgctt
     3361 ctacccgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt tcagctaaag
     3421 cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact gtattaatca
     3481 tcgcactagg caatatttcc tttatttcat agaagaattt gtgacaatca ctcccgacga
     3541 aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta
     3601 catcttcaag tacaatttct aatctatttt ctttccagcg gtatgcccca gcgtatacat
     3661 tttttcttcg agcatcaaac atgggcacaa taattttatc cacaccgatg caattagccg
     3721 cgatggtttt cagactagat actcctacta gctctttatc taatgtataa gcaatcgttt
     3781 ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca gaaaccgcga
     3841 ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg attgcaggca
     3901 tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt
     3961 cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat gtaatcatat
     4021 atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt tttattctga
     4081 ggatgaaaat accaaaggca aaactagtac aatgatacat gattatctgc tggcgggcaa
     4141 atttt
//
LOCUS       DACDHJ010000190         4142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDHJ010000190 DACDHJ010000000
VERSION     DACDHJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.193"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..1803
                     /locus_tag="IV223_002265"
     CDS             40..1803
                     /locus_tag="IV223_002265"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8712523.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            1887..2345
                     /locus_tag="IV223_002266"
     CDS             1887..2345
                     /locus_tag="IV223_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8712524.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2454..3137
                     /locus_tag="IV223_002267"
     CDS             2454..3137
                     /locus_tag="IV223_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8712525.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3284..3898
                     /locus_tag="IV223_002268"
     CDS             3284..3898
                     /locus_tag="IV223_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8712526.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARLE"
     gene            3914..4120
                     /locus_tag="IV223_002269"
     CDS             3914..4120
                     /locus_tag="IV223_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712527.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1466 a          673 c          875 g         1128 t
ORIGIN      
        1 tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt attacatgta
       61 ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga aatgaaacag
      121 ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt
      181 ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt tgattcacca
      241 aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct tttaactggt
      301 cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc caaccaacat
      361 gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag cttagcaaca
      421 ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc atttatcggt
      481 gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg tgttttacaa
      541 tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa tcatggcagt
      601 ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa taacctattt
      661 aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga aacggtgatc
      721 cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt ccatacggaa
      781 aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg gcatgtacca
      841 tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt taatatgatc
      901 atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat
      961 gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc agatcattat
     1021 tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt ggcttctcag
     1081 ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata tgatcaatta
     1141 tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg cggaacgatc
     1201 tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc aagtatcccg
     1261 aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct agattgggcg
     1321 ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt agaacatggt
     1381 acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa tggaggaaaa
     1441 gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt aaaagctttt
     1501 agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga aaatgacgta
     1561 gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga agatggtagt
     1621 ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc aatgaacgta
     1681 ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca gctttatgaa
     1741 cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa
     1801 taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat ggttttttca
     1861 ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat gtcaaaaagt
     1921 tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca tttggggata
     1981 cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag actggtacat
     2041 caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt ggtcaattag
     2101 acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata aatgtgacca
     2161 tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa ggggatcgga
     2221 cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat aaagaagcgt
     2281 tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa gtaattttct
     2341 tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa tagaaaaggg
     2401 ttacattttt atatgaagct gtatttttat ataacattac tggaggagat attgtggata
     2461 cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa aacgagccgc
     2521 tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc ggagcagttg
     2581 gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg
     2641 taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact gcgacagaaa
     2701 aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat gcaacttctc
     2761 gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac gaacacggtc
     2821 ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt
     2881 ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag acgcagacac
     2941 ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta attatggaag
     3001 gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct ttttcagtcg
     3061 tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa
     3121 gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa gaaaaattga
     3181 tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca ttttgtcttc
     3241 aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg aacggagtcc
     3301 ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca tccctagacc
     3361 agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac ccttcagtta
     3421 cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga gaaaacaaga
     3481 tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta tccatatcct
     3541 tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta atgaggaaag
     3601 cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag taccagcatt
     3661 gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga tacggaataa
     3721 aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt taaatgaaac
     3781 agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg taagccgttt
     3841 ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc tcgaataaaa
     3901 aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa aagaaatgaa
     3961 aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag ttccgccatt
     4021 gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag aaatgccaga
     4081 tcaaacgatt agcggattgt ttaattactt taacaaataa atatttattc cgaaaagtga
     4141 aa
//
LOCUS       DACDHJ010000191         4103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDHJ010000191 DACDHJ010000000
VERSION     DACDHJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.194"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..1383
                     /locus_tag="IV223_002270"
     CDS             82..1383
                     /locus_tag="IV223_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8712528.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1410..1670
                     /locus_tag="IV223_002271"
     CDS             1410..1670
                     /locus_tag="IV223_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8712529.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2669..3904)
                     /locus_tag="IV223_002272"
     CDS             complement(2669..3904)
                     /locus_tag="IV223_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8712530.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1169 a          663 c          855 g         1416 t
ORIGIN      
        1 taattatata attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt
       61 tattgaaaag gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc
      121 ccaccaatgg caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt
      181 tcaacattgt cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt
      241 cctggatgga atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg
      301 acgatgggat tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca
      361 tatggattag atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa
      421 gttcctgtaa acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt
      481 tctttgggag catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta
      541 tatcgtttct ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct
      601 gttgcccgta gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg
      661 attcgaagcg tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta
      721 accaatattt tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg
      781 ttgtggtcat ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg
      841 ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta
      901 ccctatatcg ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca
      961 acgatcgtgg tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt
     1021 ggtcgttttt ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct
     1081 cctatgatgt tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact
     1141 attatcagtt attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca
     1201 tggacattac cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt
     1261 atcttgatca tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct
     1321 tatgataaaa agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc
     1381 taagtttggt ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta
     1441 tctttttatt aacggctagt caccttatat tgaatatgct agctacatat catattcaaa
     1501 tcaatcgatg gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg
     1561 taccgcatat gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta
     1621 tcaattttat gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa
     1681 agtggttgta acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt
     1741 cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg
     1801 caagcttttg accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc
     1861 cttttttaca taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa
     1921 tggaaatttt atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc
     1981 aaatatagat ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg
     2041 taatatgtat gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct
     2101 gggaatagca atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga
     2161 acattctgac atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc
     2221 atcggattgc gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg
     2281 gtaaatccta catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa
     2341 gcgatgcttc tagatctata tgacacattt taagaaaatg tatttgccta attattatgg
     2401 tctttctttt gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac
     2461 taagaataaa tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta
     2521 aggatgattt tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact
     2581 atttactttt tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct
     2641 ctattaaaaa agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga
     2701 tttcttgaat tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct
     2761 gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca
     2821 ctggttttgt gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt
     2881 gctcaaattg catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg
     2941 tcccttcccc gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca
     3001 gatattctgt tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa
     3061 taggtgcaat aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca
     3121 tacgcggatt gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct
     3181 ttcgctgtat aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg
     3241 gaccaacgat tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg
     3301 tgatgagatt tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt
     3361 ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt
     3421 ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca
     3481 gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag
     3541 gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc
     3601 gaggctcaaa cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt
     3661 tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt
     3721 ttttcgtaag gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag
     3781 caaaagtttt agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa
     3841 cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct
     3901 tcatgaaatc ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa
     3961 aacacagcgt tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc
     4021 acaacttcta aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc
     4081 aacaattttt tgatcgattt ata
//
LOCUS       DACDHJ010000192         4099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDHJ010000192 DACDHJ010000000
VERSION     DACDHJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.195"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IV223_002273"
     CDS             641..1375
                     /locus_tag="IV223_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8712531.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IV223_002274"
     CDS             1391..2560
                     /locus_tag="IV223_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8712532.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IV223_002275"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IV223_002275"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8712533.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..>4099
                     /locus_tag="IV223_002276"
     CDS             3650..>4099
                     /locus_tag="IV223_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8712534.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGV"
BASE COUNT         1385 a          689 c          892 g         1133 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcagacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagta
//
LOCUS       DACDHJ010000193         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDHJ010000193 DACDHJ010000000
VERSION     DACDHJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.196"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1673)
                     /locus_tag="IV223_002277"
     CDS             complement(<1..1673)
                     /locus_tag="IV223_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712535.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHN"
     gene            complement(1633..3387)
                     /locus_tag="IV223_002278"
     CDS             complement(1633..3387)
                     /locus_tag="IV223_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712536.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3497..>3956)
                     /locus_tag="IV223_002279"
     CDS             complement(3497..>3956)
                     /locus_tag="IV223_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8712537.1"
                     /translation="SPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAY
                     LPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQD
                     WIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1335 a          757 c          561 g         1303 t
ORIGIN      
        1 tcattatgag taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc
       61 ttttcaatag acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga
      121 tttaaacttt cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct
      181 aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct
      241 cctccagaaa gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt
      301 tcaataaagt cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct
      361 tgactatttc caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga
      421 aaaacaatag cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca
      481 tcaattaaaa tacagccttc atcataatca tacagtctag acactaattt caagatacta
      541 gttttcccgg aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa
      601 gagatatgat ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca
      661 atgtcaaaag atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct
      721 ctaattcgct gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa
      781 tcgaaggaat cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc
      841 acttcacccg ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat
      901 gagaaaatag aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc
      961 ctttcactct cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag
     1021 ctattaatct cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa
     1081 gtatcgtaat atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca
     1141 aaactaaata acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc
     1201 atcactgaaa taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc
     1261 atcgcatgct caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta
     1321 tgttttgaaa aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa
     1381 tgcgcacttt ctttataagt actattatat aagttttcat actccctatc taacaaccaa
     1441 tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt
     1501 tctaaaacta attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag
     1561 tatacaaaaa aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa
     1621 gcaaatttgt ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata
     1681 attagtgacc aattgtttat acacttgatg tttagccatt aattcattat gatttccacg
     1741 ttctactatt ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa
     1801 tcgatgagct atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag
     1861 ttcatactca ttatctggat caatagatgc actggcttca tccataataa caatctttgc
     1921 atcttttaaa attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac
     1981 tcctttacta ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca
     2041 accggctaat cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga
     2101 aacgttatcg taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt
     2161 gttaataatt gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc
     2221 cttatccaca ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga
     2281 ttttcctact aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac
     2341 acactcttct ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg
     2401 tgtctcgttc ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt
     2461 ttcgactgca tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga
     2521 aacaaaaata attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg
     2581 agtggtaaga gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa
     2641 aaatagccat tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa
     2701 cttagcataa tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa
     2761 tatctttata acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa
     2821 cgattcttga tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac
     2881 accaattact aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa
     2941 ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat
     3001 tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt
     3061 taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt
     3121 agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc
     3181 tcctaaagtt aaccaagcaa cagtttgaac cgcataactc ttagctaaat attcatctcc
     3241 tacaataatt agagactgaa gaaatttgta aatactgtaa tatccataga ctgttaatac
     3301 gacagaaaaa tcagaaaata aagtggctaa ccatccgtag tatttcattt tagggacata
     3361 actaaataat tttttgtata ctttcatttt ttcctccccc gcattcaatt tcttctctta
     3421 ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat tagagatgta caaacagata
     3481 aaaggtaaac tacgttctat tttttgtatt tttttaagac ttttcttcca atcaatacac
     3541 caatcataga taaacttgct acaactaaca ctgaaatgat tccccatata cctaccgcat
     3601 agtggatcat tgcatccatt gttgcagcag attgtccttt atctatccaa tcttgacgaa
     3661 aacgatctgt aaagaaccat ataggaatcc actgtccggc agcactgcct aattgcatta
     3721 gagcccaaga cgcaacttga ggaattgctc gatcatatcc aaatacagat agaataatat
     3781 cagctaagat cgccatacta atcgaactga taaaccaagg taagtacgcc ccgcccatta
     3841 acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa aattcctttt ctagggtagt
     3901 tatagctaat ataaacaaat atgattccag ataataatcc aaaaatcccg ggggat
//
LOCUS       DACDHJ010000194         3916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDHJ010000194 DACDHJ010000000
VERSION     DACDHJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.197"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..1530)
                     /locus_tag="IV223_002280"
     CDS             complement(25..1530)
                     /locus_tag="IV223_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8712538.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="IV223_002281"
     CDS             complement(1527..2213)
                     /locus_tag="IV223_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8712539.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IV223_002282"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IV223_002282"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8712540.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1321 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atattt
//
LOCUS       DACDHJ010000195         3895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDHJ010000195 DACDHJ010000000
VERSION     DACDHJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.198"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..181
                     /locus_tag="IV223_002283"
     CDS             <1..181
                     /locus_tag="IV223_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712541.1"
                     /translation="GTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFT
                     FSKQKQPRREVQHSH"
     gene            complement(326..1066)
                     /gene="truA"
                     /locus_tag="IV223_002284"
     CDS             complement(326..1066)
                     /gene="truA"
                     /locus_tag="IV223_002284"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8712542.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1257..1892)
                     /locus_tag="IV223_002285"
     CDS             complement(1257..1892)
                     /locus_tag="IV223_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8712543.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2039..3280)
                     /gene="sstT"
                     /locus_tag="IV223_002286"
     CDS             complement(2039..3280)
                     /gene="sstT"
                     /locus_tag="IV223_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8712544.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3478..>3895
                     /locus_tag="IV223_002287"
     CDS             3478..>3895
                     /locus_tag="IV223_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712545.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVS"
BASE COUNT         1293 a          804 c          592 g         1206 t
ORIGIN      
        1 tggcacaact gaattaacta ttcctccagg tataaataaa gtcgttatta cttatcagta
       61 cacaaaacta gcaattgctt caagatattt atctatcgtt actttgattt tatttttact
      121 ttatcgattt actttttcta aacaaaaaca accgagaaga gaagtacaac attctcacta
      181 gatttttatt taagaaagac tttttagctg tacacataaa caggctaaaa agtctttttt
      241 tatatttctt taatttgctt catacattat ttaacatcga tatttatact gtgaaacatg
      301 cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa aacagacctt
      361 gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt tcatcgattg
      421 tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata atccgcacca
      481 ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc tttcgttcga
      541 tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat gcagaaaaac
      601 caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg tccatatcca
      661 actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc
      721 aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag cgctcaggca
      781 tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata aagtcgatca
      841 tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg
      901 tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc atctgatgca
      961 atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt tgccatcctg
     1021 aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt tagtggctcc
     1081 tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa atatagctcc
     1141 ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt tcagaaaaac
     1201 aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct caagttttac
     1261 tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa aaaactgatt
     1321 ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg aattcttgcc
     1381 agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta taagtggctt
     1441 acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa caataaaaaa
     1501 ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt ccacgaagat
     1561 tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc atagaacgcg
     1621 atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc agcatacaat
     1681 gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag aaacgctttt
     1741 cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg atctaattca
     1801 ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc taataataaa
     1861 aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca attacctatg
     1921 cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct atttacacga
     1981 acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata tctaacgttc
     2041 aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca cttcttttac
     2101 gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt aaggcagttt
     2161 ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc atcgcgacat
     2221 cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa
     2281 cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa
     2341 aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa atggtaatcg
     2401 cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa gactcttttg
     2461 tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca gaacttcggg
     2521 taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga tagggatttt
     2581 ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac agcatcgtac
     2641 cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtagcaa
     2701 ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc acttgtacga
     2761 cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct gaacgtgctc
     2821 gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc gcatcaataa
     2881 gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca ctttccaaac
     2941 tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac
     3001 ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag atcaaaatag
     3061 gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc gtcaagaaaa
     3121 aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc aatattgaaa
     3181 taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata ccaacaacaa
     3241 tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc tttcacaatt
     3301 ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc taaaaattct
     3361 aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca cgttgttttt
     3421 tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg ttttcttatg
     3481 tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa ggagagttat
     3541 ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat attgatttca
     3601 gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta tacattttta
     3661 caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta ccaatatcaa
     3721 aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat attcctaata
     3781 gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa tattcgtgat
     3841 tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt ttctt
//
LOCUS       DACDHJ010000196         3850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDHJ010000196 DACDHJ010000000
VERSION     DACDHJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.200"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(93..635)
                     /locus_tag="IV223_002288"
     CDS             complement(93..635)
                     /locus_tag="IV223_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8712546.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1158
                     /locus_tag="IV223_002289"
                     /pseudo
     CDS             770..1158
                     /locus_tag="IV223_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1162..1926
                     /locus_tag="IV223_002290"
     CDS             1162..1926
                     /locus_tag="IV223_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8712547.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2055..3002
                     /locus_tag="IV223_002291"
     CDS             2055..3002
                     /locus_tag="IV223_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8712548.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3132..>3850
                     /locus_tag="IV223_002292"
     CDS             3132..>3850
                     /locus_tag="IV223_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712549.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLG"
BASE COUNT         1276 a          658 c          786 g         1130 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggtgc tatggcgatc aggaaattta
     1141 atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt ccatcatatt
     1201 ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt tgtcatctat
     1261 tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa aattgcgcag
     1321 cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa acagccggtt
     1381 tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt tgaatttgac
     1441 acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc agttggcggg
     1501 gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca tatagtagcg
     1561 ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc
     1621 gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc atgggttaag
     1681 agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt cttttggcat
     1741 ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga aaaaaatcgg
     1801 cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg acatatggta
     1861 caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat aaaaaagtat
     1921 aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt ttattttggg
     1981 ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga caacatgtta
     2041 gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat gtttcaaaaa
     2101 tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg aaagagttag
     2161 ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa gcacttgtca
     2221 cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca actgagccct
     2281 attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat tattctctcc
     2341 aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc atcacaggtg
     2401 tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc ttgtttgggg
     2461 aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca gcaaaagcaa
     2521 cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc gaagtggacg
     2581 agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa gttgatcttc
     2641 ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat atgacagaaa
     2701 actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag
     2761 gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt ggtgtgattg
     2821 gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg atcaagcaag
     2881 atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag
     2941 gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag acaacgaatt
     3001 aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa aataatcagc
     3061 gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa
     3121 ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg
     3181 agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc
     3241 ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca
     3301 cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc
     3361 gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat
     3421 cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac
     3481 aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg
     3541 tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta
     3601 gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt
     3661 atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg
     3721 tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat
     3781 cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa
     3841 actctttggg
//
LOCUS       DACDHJ010000197         3798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDHJ010000197 DACDHJ010000000
VERSION     DACDHJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.201"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            77..574
                     /gene="dfrG"
                     /locus_tag="IV223_002293"
     CDS             77..574
                     /gene="dfrG"
                     /locus_tag="IV223_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8712550.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(646..2598)
                     /locus_tag="IV223_002294"
     CDS             complement(646..2598)
                     /locus_tag="IV223_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8712551.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2605..2901)
                     /locus_tag="IV223_002295"
     CDS             complement(2605..2901)
                     /locus_tag="IV223_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712552.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3079..3798
                     /locus_tag="IV223_002296"
                     /pseudo
     CDS             3079..3798
                     /locus_tag="IV223_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1153 a          667 c          630 g         1348 t
ORIGIN      
        1 gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta aattagttaa
       61 aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga atagagtgat
      121 tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg ttaaaaatac
      181 tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg gaagagcctt
      241 acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta atggttgtga
      301 aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag aaatttttat
      361 tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa tgtacatcac
      421 aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt atgaggaatg
      481 gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata actactattt
      541 tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga agaagaacag
      601 taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt tgaaataaat
      661 tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag gtgtcaaaac
      721 ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta gaattgtaat
      781 cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct tatcaccacg
      841 agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt gaataaagct
      901 attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg gttccagagc
      961 agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg ctaatggatg
     1021 tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa tagcctcttc
     1081 gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag tccgtagaaa
     1141 ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg cttgtccgaa
     1201 ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa aatgatgtgc
     1261 atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa tagagaagtc
     1321 atcatcacta atcattcgcc attcgtcggc attaaccatt tgtttaatta accaatagtg
     1381 agcagttgta gtataaggag catcaacatt taaaccatcg acaaatctac tcctattagt
     1441 aattttccct aattcgtgaa gataagcatt cctattttcg ctatcgttag cggatggttc
     1501 ttgtggataa taagaccaat ctaacctatc atttttacga ctaaaagtat tgagatggtc
     1561 ttctttgaat tttcttgtat cttcatttaa ttcatccatt gaaatattcc aatcataggc
     1621 aacatcagaa cggaacacat atcttgataa gacctcagca gatacaacta tatacgtttg
     1681 catattaagg tcttcaaacc ccgtatattt ctttgaacct tgtcctttct tacggacgtt
     1741 atttaggtag tagtgcatct tgtccgtatt aagccacatc tcgttaaatt tctttgtttg
     1801 aaggggttgt gtctcatatc gttctaaaaa ctccaaacaa cgcctataca gccactccag
     1861 cttatgatag taggttccaa ctccaattcc taaaatatca caagtacgac tgactgaaac
     1921 ccttccaaca accatcttag cgaacattgg aagaatggtg ttcttttgtt ggtgataagt
     1981 agtggtttct tctctttttg gtagaacatt agtgaacttc ttacacgcct tacattgata
     2041 gcgttgagat ttgcccctac ttttccccct tttgtaaaat tgcttcggct cattgaatgg
     2101 ggttgattct tcttccgagc agccttcttt atgaaattga taatctggct caacatcttg
     2161 gatgctattg atttctataa ggcgtttaat ttcttcaaca acagaccaat tagataaagg
     2221 ggttactgta cagttttgcg atacacctct gccaatagga ttgtcattgc agtacatacc
     2281 tttatgacct ttatctttgg aactaccagt cattttatat cgacttggtt tcccttttac
     2341 atctttgaat ttgtgctgtt ctttcccaaa attacaacaa aacggattga tgcagtaatt
     2401 atattgaatg ggatattcag aaccgttcca tttaaaagac gttggcaaga acaacatttt
     2461 gttatacctt gttgcaaatc gttttggagt taataaatca tattgtttag ccctatcttt
     2521 taattcttcg tttgaaatcg gaattttaac atctacaatt tcatcttcct tcgtggctaa
     2581 tcttttaagc ttggtcattg tctatcaacc cactctatct ttgataccca ttttttcaac
     2641 aatccgctta ttacttcgct tgttggcaat ccattcaata aacttcttat cgtctaaaat
     2701 gttcaataag aattcatcca ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg
     2761 cttaactatc acccttactt gctctgatat ttcccaatcc acagacttag cgttcttatt
     2821 ttttggctca agaaattgca taggaaaatg cacccctttt tgcttagtaa attgtaacca
     2881 ttttaacaca ccaatttgca ttcctacaat aattatttca cgttcgcatt tttatataca
     2941 ttttaactta cattttgtgg tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg
     3001 agtatcaaat tcagttgcaa tttgatactc atttaggttt acattttcac tttttgtact
     3061 ccaaaactga actacttatt tttatttagt tgtctctata ttttctctgc ttatcggaca
     3121 gtagacatct actatcgttc cgcaattgcc gaagctatcg caataacact tgttatccct
     3181 gtattatttt atgcttatca aatcatctct ggaaaagaag aaaagtatcc gtctgtaaag
     3241 ctagcattga gtatgagttt gctagtgtac agccatgtac tatcaacatt aatgagtaca
     3301 gcattaatta tcatttttat ttttattcgt ttggtttcta aaggtttcaa gaatgcagat
     3361 tttattgcta tatttaaaaa attatttagt gcggcaggaa tgacattggt tctgacgagt
     3421 gctttttggt atccgatgtt tgaacaaatg ctctaccaaa aaatcaacaa acctagcgtg
     3481 acaaatttat atgctcatgc atctaatgtg ttcgatagtc tgactgaagc tatgaacaac
     3541 gacttaacta cttactcaat gggactagtt ggtctgttat ctttatgtat tcccctgatt
     3601 ttatttaaaa aattaacaaa catagaaaaa aagatttatt atggtacgtg tttgacttgg
     3661 ctagctacaa ctagtttggt gccatggtat cttctgcaaa atacgccagc caaattgtta
     3721 caatttcctt ggcggatctt aagcttgcaa ataatattct cttctctgat attaacgatg
     3781 atatttttca aaaataga
//
LOCUS       DACDHJ010000198         3786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDHJ010000198 DACDHJ010000000
VERSION     DACDHJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.202"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..579)
                     /locus_tag="IV223_002297"
     CDS             complement(<1..579)
                     /locus_tag="IV223_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8712553.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLL"
     gene            725..1639
                     /locus_tag="IV223_002298"
     CDS             725..1639
                     /locus_tag="IV223_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8712554.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            1687..2157
                     /locus_tag="IV223_002299"
     CDS             1687..2157
                     /locus_tag="IV223_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8712555.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            2242..2850
                     /locus_tag="IV223_002300"
     CDS             2242..2850
                     /locus_tag="IV223_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8712556.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            2984..3184
                     /locus_tag="IV223_002301"
     CDS             2984..3184
                     /locus_tag="IV223_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8712557.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1267 a          555 c          749 g         1215 t
ORIGIN      
        1 tagcaaagtg atcagcagat tcagaaaact aggataaata gaaaatataa atgtcaggac
       61 ttttatccaa agtggtatag atgcttcact taaaaaagaa cggagctgct tacctgttaa
      121 gttttttaaa gattcattgg tgtagggaag ttctaattgg ttattgttca acaaagcaga
      181 agtcgtgcct gtattaggaa gagctatgat aagtttgttt tttaacatcc cgacactaac
      241 aaaattcccc atcaaatcag tagagatgtc ctgttctgtt cgttttccct ctggatcaaa
      301 tgtgaagata atggagtttg tttggtaaat gaatccttct ttttcttcgg tttgaagctg
      361 cccgttttgg attgtaaaat caggaatgcg agtagcaatc ttttgcccat ccaattgaat
      421 gttgtttaag acttgaaaaa cttgaaaaga gattggcaaa gccataatta tactcaaaac
      481 taaaagatag acaatggctt tccaaaatgg gacatttctc gcctccttta ggtctttgaa
      541 tcgtataaag gaactgataa ttaattgttt tgttgtcata agtactcctc atctagtagt
      601 gtcatcactt gcaattctag cgaagtatca agtgaaatac aagtgcttta aggaaacagc
      661 tgaatgattg gtgaaatatc aaaaaaatgt tattcttgtc taaagactat ccaatataaa
      721 taaaatggag gaaggaatag tgcctacaca gccacagaga tcatcaaaaa aactatttgg
      781 agtagtggtt ttgaatgcgg tgatcacttt atcagaattt attggaggga ttatgtcagg
      841 gagtttgtct cttgtttcag atgctttcca taaccttagt gatacattgg caattatttt
      901 ttcttacggg gcacaaaaaa tggcccgaaa agaagcaaat aaaaaacgta cgtatggcta
      961 ccagcgttta gaaatattga gtgctttcat caactcattc atattgatca tattatcctt
     1021 gtttttaacc gttgaagcat tcaagcggtt cacttcacca gaaaagataa atagtcattt
     1081 gatgctaaca gtagcagtta ttggattgct cgcgaatttg ttctcagtat tattattacg
     1141 acaagaagca gatgaaagtt tgaatataaa atctagttat ttgcatttgt taagtgatac
     1201 actgtcttcg gtaagtgtga ttatcggagc aattttgatc cgttttttcg atatctactg
     1261 gatcgatcca gttattacac tgatcatttc gatatatata ttgatagaag cgataatggt
     1321 gatcaaaaaa gctgccgcta ttttgataca aagtgcacct gctatcgact atgaaaaaat
     1381 ggaacaagaa attgaagcaa tcgatggggt aaaggatgtt catcatgtcc atatttggca
     1441 gtatagcgaa aagataatta ttttcgacgg acatattgat tttgaagatc agctactaag
     1501 cgagattgaa aagatatatc ctcgtataat ttctttatta aaattaaaat atggtattac
     1561 acatgtgacg atccaagcag agacaaaggc aaaggatcaa aaaaaattaa tttttttaaa
     1621 taaagatgaa agttattgaa atatagagta tgataaataa gaatttttaa taaggtaggt
     1681 tggctcatga agcagtatcg acagttaaaa ggatatttag aagcaaaagg atattgggag
     1741 atactagtct atctcttttt tggtggtttg gctacgattg taaattttgt tagttttgct
     1801 ctcgcacgcc aatattttga tttaagtatg gccttatcca attctatttc gtggttttgt
     1861 tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa atcaccgaat
     1921 tttacacatg cacttattga atgcggtaag ttcttcttct accgtatcct ttcttacgga
     1981 ttggacatgg gggcaatggt attgttgatc aatgttatga acagcaatga gtatgtggcg
     2041 aaaatcatca ctcaaattat tgttatcctt gcgaactata ttttcagcaa gttgtttatt
     2101 tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga cgactaggca
     2161 taacaattga agaatttcct tctctttgtt actctttgta atgtagacat tattaagaaa
     2221 acattgagga ggaatttttt tatgacttac acattaccag atctacctta tgcatatgac
     2281 gcattggaac cttatattga cgaagaaacg atgcatctgc atcatgataa gcatcacaat
     2341 acttatgtga cgaatttaaa ttcagcaatt gagaaatacc cagaattagg cgaaaaaaca
     2401 atagaagaat tattatctga tatggacgct attccaacag atatcaagac agctgtacgt
     2461 aacaatggtg gcggacatgc taaccattca tttttctggg aaatcatggc accaaatgct
     2521 ggtggcgaac ctacaggaga aataaaagaa gcgattaatg aagcttttgg tgatttttct
     2581 tcttttaaag aagaattcaa aaaagcagcc gctggacgat ttggttctgg atgggcttgg
     2641 cttgtaatgg aaaatggaaa attagctatt acctctactg caaatcaaga ttctccattg
     2701 atggaaggaa agacaccaat tctaggtttg gacgtttggg aacatgctta ttatttgaaa
     2761 tataaaaatg tccgaccaga ttatattgca gcattctgga atgttattaa ttgggatgaa
     2821 gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc cgtcatatca gtcttaaaaa
     2881 taatttggaa aataaaagag cgaaagcttg ctagcgtttt ctaaattggg tataatgttc
     2941 ttgcaaggtt acttgcaaga acttttttgg aggatataca tttatggaac acggaacggt
     3001 taaatggttc aacaatgaaa aaggatttgg gtttattaca gtagacggcg gagatgacgt
     3061 atttgttcat ttctcagcaa ttcaaggaga cggcttcaag agcctagaag aaggtcaaga
     3121 agtagaattc acgattgttg aaggtgcacg cggacctcaa gctgctgaag tgactaaatt
     3181 ataataattt gttgaataaa gacacgactt cttagagagt cgtgtctttt tgtctattta
     3241 tgaagttttt agattttttg gctaagtaaa tgcatttgtt cacttgacat gtcctaacta
     3301 attatcaagc aaggaacatt ttcttcgtta gtaatggatt ttaaagaaat ttcagttttt
     3361 ggatgttgaa taaaagacca tttgtgaaac cactagtata catactcatt accatgtcaa
     3421 ttctattact ttaaaagaaa atcgttttac tttgaagagg agcatatcta tccattaaaa
     3481 gatgatggta atgcaatcac tagttcgagt cattttaaga gccatgcagt gagctggaaa
     3541 taaaacgaat agaagatggg tttaacaaaa ggaccagtca taggaagaaa ttgtcgtgaa
     3601 atcgaatgat aatctgaacg gtaggcaaag actgttaaaa agatgggaaa ggtatgtgcg
     3661 atcattttac tgcacgatta tttggaaaat accttgttgt tcgctttttc tggaaaaaat
     3721 acataaaaaa agataaaacg attgcgaact gcgtgtattc ggtctttcga tctttttcac
     3781 ggtatg
//
LOCUS       DACDHJ010000199         3694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDHJ010000199 DACDHJ010000000
VERSION     DACDHJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.203"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..196
                     /locus_tag="IV223_002302"
     CDS             <1..196
                     /locus_tag="IV223_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP8712558.1"
                     /translation="KKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFANGEQLEMG
                     KRSIDKLKNFMQEKIENESF"
     gene            372..1115
                     /locus_tag="IV223_002303"
     CDS             372..1115
                     /locus_tag="IV223_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712559.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1108..2688
                     /locus_tag="IV223_002304"
     CDS             1108..2688
                     /locus_tag="IV223_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8712560.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2939..3418)
                     /locus_tag="IV223_002305"
     CDS             complement(2939..3418)
                     /locus_tag="IV223_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8712561.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3434..>3694)
                     /locus_tag="IV223_002306"
     CDS             complement(3434..>3694)
                     /locus_tag="IV223_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8712562.1"
                     /translation="KFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQ
                     ITNAIRDLLAGHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
BASE COUNT         1178 a          630 c          693 g         1193 t
ORIGIN      
        1 taaaaaatca ctcgaatatg agtatttatg taaagattta aaagcttata tcattaatac
       61 tgaaaacatt acatctatta gcagagtaaa cgaaatagta acatttgcaa atggagaaca
      121 actagagatg ggaaagagaa gtattgataa actaaagaat tttatgcaag aaaagataga
      181 aaatgaatca ttttaaagaa aacaaagact catatcttac ctgttagaag agtgttttcc
      241 tgtagaagag aacaacccaa gtagcagcct agtcgtttct atcctttttt gttatgcact
      301 ctttttgaca tttactccac caataacgaa gggtacaata aaaagtaata acaaacaaaa
      361 ggagtacaga aatgattgaa ttcatcaatg taagtaaaag ttacgggaaa aaacaagcat
      421 taaaagactt gtctctctcg ataagacaag gggaaatttt tggattttta ggacataatg
      481 gtgccggaaa atcaactacg attaaaagtt tagttagtat tattgaacca tcatcgggaa
      541 cgattttagt agatggaatg aaattgacgg aaaatcgatt gagtatcaaa caaaaaatcg
      601 gttatgtgcc tgattcccca gatatttttt tacaattgac ggctggagaa tattgggact
      661 tgatttcagc tgcctatgaa ttagacacac aaaaaaaaga aaagcgttta gcagaattga
      721 cagcgctttt tgatatgtat agtcatcaaa atgaaacaat cgcaagtttt tcacatggaa
      781 tgcgtcaaaa aacgattttg atcggcacgt tgctgccaga tccagatatt tgggtattgg
      841 atgaaccttt gcaaggactt gatcctcaag cagcatttga tttgaaagaa atgatgaagg
      901 ctcatgccgc aaaaggaaaa acagtcattt tttctacaca tgtattggat accgcacaac
      961 agttatgcga taaactggct attttgaaga aaggtgaatt gatttatcag ggggcagtta
     1021 gtgaattatt gaatggctca cctgatgaaa cgctagaaaa gatttatttg aaaatggctg
     1081 gaaggcaagc gacaggagat ggaccgtatg tataagcgac aattattaga attaattcga
     1141 gttaatcttt tatatgttaa tccacagctc acgaataaag caagagagaa aggaaaaaag
     1201 ggaaagcgat taactatcta tcttttgtct caaaatcttt tttcagggct gattttttta
     1261 ctgatttacg gatttgctat gtttgctatg aactttgtta aattgccagg tttctttact
     1321 tattacgtgg cactttttgg tatgttaggt ttaactcaaa gtatatcagt catttataat
     1381 atcttttttg aaggaaatga cctgcaagta tttttgcctc tgccttttgg acaagaacag
     1441 gttttttcag caaagatttt ggtagttgct ttgaccatca ttccttttgt ttttccgctt
     1501 ctagtcatgt ttattttaac cggatggcga gctgatctag ggattttgct tactatcatt
     1561 atctcaatcc ttttgttttc tctatttttg attattcttt tttgtctggc tagtttgatt
     1621 gtttttggtt tggctcgaac agcagtgttt aaaaaacata gaaaaacagt ttctagtatc
     1681 ttgcttggca gctcgatgct gatcgcattt gctggaatca tgtggatgaa tcatcaaacg
     1741 gggactattg aaaatgagtt aacagatcgg catccgattt cgatattatt gccattctat
     1801 gatgctgcca gtcagccact ttctcaacaa ggtttattgg gttggggaat actagctatc
     1861 acggctctgg gatttttcct tgctgtaaaa cagctgatag taccaaagct tacagagcaa
     1921 ctgactgatt caaacagtga gttcaaaata aaaagaaccc ataaaaagaa ccaaaatgtg
     1981 catcagcttt tatttagtta caatgctcaa ttagtaaaag aacctaattt gattatgcaa
     2041 gttctttctt cttccttgtt aactccaatc atttttattc ttgcttttgc cctaggaggc
     2101 gaaataaaat taactgattt agataataag ttaatcggtg ttgttttcct ggtagggatc
     2161 gcattggctt tcttaacagt taatcagact tcttttatca gcaatcttat ttctttggat
     2221 caagaaaatt ttctgtttat ccgttctttg ccaatctcta tgaaccaata tttaaaagaa
     2281 aagttcagat tcggatggat attgcagagt tttttaactg gtgggatcgc attattaagc
     2341 ggactgtctt ttcagctgcc tttgtttttt atccctagct tgctatttgg ttctttattc
     2401 ggctgctact tattgtcact acgttatttc gctagagatt atcggttgct gctgctgaac
     2461 tggaccaata ttaaccaatt atttaacaga ggttcaggaa gtttgggttt agtcgcaact
     2521 atgatgggtt cattgatcat cagtattatt cttatcgtct tatacggaat gttggctttg
     2581 ttttatccat tttggttagt gaatattcct gtgatcggtt tagttctaat actttccgct
     2641 ctatggatcg tttattatca acgaacgttt tggaaaaaat ttgaataaag aagaggggcc
     2701 gtgacaaaaa tcatgtcgca gccccttttt tacagataga gggtgttcaa aagtcagttt
     2761 ctcagtatta agtcaatttt atgtaaaaat gtgagaagct atttttacaa aacttttttt
     2821 aatactcata attacttctt attgctcctg cggttacttg tcgcaatgtt tgtgagatat
     2881 tttgtcatcg ctcaaggctg aacgtctctt tcacaacctt ttctttgtta cacttcgttt
     2941 atttaaaaag ccgttttttt cgtttcatca tgattgcgtc acgctttact ttgaaattac
     3001 ggatgttatg gttaaagact tctgataata caagacctaa agcgattgct ccagcaatca
     3061 taattgcttg gaccgcataa tgagccgctt ctcgatagga ttcagtaacg agatttcgaa
     3121 ccgcttgata agctaaacca ccagggacta gaggaaccat cccgggaata ttgaaaatcg
     3181 ttacgggcat tttcaaacgt ttagagaaaa gatcgctgat gaatgctact cctaaagaac
     3241 caagcaatga accaaaagca gcagatccgc ctatttgaac acagatccag tagatcatcc
     3301 agccaaaagc accagaaaga ccacaacaaa tcaatgaacg cctaggtaca tttgtaataa
     3361 cggcaaatgc cgcagtcgct aaaaaactaa aagaaaactg aataaatatc gtccccatac
     3421 ttgtgttcct ttcctaataa aaaagctgga aaataaaagc aatcgcaaaa ccgatcatcg
     3481 tagcagtcat catcgcttct gttccgcgag aaactcctga gacataatgt ccagctagca
     3541 aatcacggat agcgttcgta atttgaactc caggaactaa aggcatcaca catccgataa
     3601 tgattagatc ctgattgctt cctaagccaa aacgtacgct gtagatagcg gctatgccaa
     3661 tcaataaaga agataaaaat tccgataaaa attt
//
LOCUS       DACDHJ010000200         3679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDHJ010000200 DACDHJ010000000
VERSION     DACDHJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.204"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..1095
                     /locus_tag="IV223_002307"
     CDS             4..1095
                     /locus_tag="IV223_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8712563.1"
                     /translation="MFVTLFLILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKG
                     RGPFSDEIAQYAVGAAVKYKLLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCL
                     FPKGTARGIGGIEGGWYMNFPNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGR
                     GGYAAAGITEDSPYYTGCMSIITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPG
                     SSLDKSTFSGGQLFGTNPGSEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPG
                     ISGLGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTR
                     MDWRQAQGHAFTDDGTWIDPLPFLNSSKK"
     gene            1111..1755
                     /locus_tag="IV223_002308"
     CDS             1111..1755
                     /locus_tag="IV223_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712564.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNSSSAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVNEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            1766..2572
                     /locus_tag="IV223_002309"
     CDS             1766..2572
                     /locus_tag="IV223_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712565.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            2595..2824
                     /locus_tag="IV223_002310"
                     /pseudo
     CDS             2595..2824
                     /locus_tag="IV223_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3370..3645
                     /locus_tag="IV223_002311"
     CDS             3370..3645
                     /locus_tag="IV223_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712566.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
BASE COUNT         1383 a          544 c          760 g          992 t
ORIGIN      
        1 actgtgttcg tgaccctttt tctaatactg gcggcgggtt cgattgataa ttctgattca
       61 gattcaagca gtggtggcga agcttttaca ggagaatatt cggaaggatt acccatttat
      121 aaagaaatca aaggaagagg ccctttttct gatgagattg cgcaatatgc tgtaggtgca
      181 gcagtgaaat ataaattatt accaagtgtg atcctttcgc aatacggtta cgaatcagcg
      241 tttggtacgt cggcgtccgc aagaaatgat ctcaactatt ttgggattac gtggttcgat
      301 ggctgtttgt ttcctaaagg aactgcacga ggaattggtg gtattgaagg tggctggtac
      361 atgaattttc ccaactcaaa agcagccttc tcctattacg gctttatggt agccacacaa
      421 tcaaatttta atgcatgtgt aggaaataaa agccctggcg caagcttatt gatcttagga
      481 cgaggtggtt atgccgctgc aggaattact gaagatagtc cgtattatac aggttgtatg
      541 tcaattatca cttctaataa attaaccgaa tatgatgaat tcgcgatgaa acattggggt
      601 gaaggtggta atgataatgg cacaattact ggtgaatgga cgaatccatt ccctggcagc
      661 agtttagata aaagtacctt tagcggtggt caattatttg gaacaaatcc aggcagtgaa
      721 ttccgtccga atggcttcca tgatggatta gattttggtt cagttgatca tccaggaagt
      781 gaaattcacg cggtgcatgg aggaaaagtc gtgtacgttg gcaatcctgg tatttcaggt
      841 ttaggtgctt gtgtgatcgt gattaattac gacgggttga atatggtata tcaagaattt
      901 gcgaatagta caggaaattc tcgtgtgaag gttggagatc aggtgaaagt tggtcaagta
      961 atcgggatcc gagatacagc acacttgcat ttaggtttca ctcgaatgga ttggcgtcaa
     1021 gcacaaggac acgcattcac agatgatggt acatggattg atccattacc atttttgaat
     1081 agcagcaaaa aatagatcag gagaaaaaat atggaaaata aaactttaaa aatcgtctta
     1141 acagctgcaa ttagcgttag tttaatcggc aatatcgcgt tgttttggca gctgaaagag
     1201 gaacaagcga caaataccta taaaattgaa gaagtggcta aaaacgcgtc tgagaagctc
     1261 aaaaaaaatt cctcttcagc gcaggcaaag atcgacaagt taaatcaaac tattaatgag
     1321 aaaaaccaag aaatcgatca gctaaagcaa caagatggca caaaagcaaa agaagaagta
     1381 aatgaagcac aaagtaagac ggcagaagag ttcggtaaat tggccattga taaatcatta
     1441 agcagaaatg aattaacgga aaaactaaaa gatgtcgcaa cccaagaagt gattgacaaa
     1501 ttaagtcctg cagacgatga tcaccaacat gatgattacg gaagttactc ctttacatta
     1561 ggtgacgtgc aaacttacgc tcaaacaagc agtgtaaaag aggaacaaaa ttttgtcgta
     1621 tttgttgatt atacaatcgg caatcctcaa ttcaaagatg tcaaaccgca aaaaataaaa
     1681 ggtggattaa ctgtcactga aaaacaagta gatggtcaat ggaaagtcac agactttagt
     1741 tatttcactc gttaggagga agaaaatgag taagaaaaaa ggagcaagac aacgtttcaa
     1801 aaaacgaaaa agttatctta gatggctggg aatctttatg attctcggct ttttaattct
     1861 tgctagtccg atttatacgc ataagaaggt agacatacca gatgaggtaa gcacgaacgc
     1921 atttttaaac acgaaggaac tgacgatggt ttcaaaagaa aaaggagcga accagcaatt
     1981 agtttttgaa tttgcgattg acgatgaaga tcaaagctta ttaaaagaac tggctaatct
     2041 taattatcgc gtagaagtaa agacagctaa aggggattat caagcaataa aagccaaagt
     2101 gattaaggtg tctgatgatt attttgcagt gaagctatcg catgtaccag aagaatatat
     2161 tgctatgcga ttaactatta ttcctgaaaa aattgatcca aaggtggata tgcaagaacc
     2221 gcaagatctg attttttata ttcacgaaga taaggtcaaa gatcacgtga aagacgaaga
     2281 ttatgaacaa catgcgatca attacaaagt gaaaggatac aagaaagaac aaaaagcagc
     2341 acaaaaacaa attgaatcct tccaagcaac cattgatttg aatgaagaat tagtgaagaa
     2401 actaaaagat caattgcctt atcaagtcgc agaagatcag gaaaatactg aaaataagat
     2461 taatagttac cagcaagaaa ttgaaacatc gaaaacgcaa atgaaagatc aagaagagag
     2521 cattcaaaaa cttcaagaaa aaattgatcg tatgacaaaa gaaaatattt agaagaatag
     2581 gtggagtaaa agaaatgaag cgagcgaact tggtcggcaa agatactttc gctgagcaga
     2641 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt caagaagtga
     2701 attatctaac acaacaatat ctgagttgaa gaatggtaga aagagtttag ataccttaaa
     2761 attcagatca tttgaagctc tatatatagc tgctaaaaaa tattttacag ctgataacaa
     2821 ataaaaaaca ggagtatttt atgtgaaaaa agtatataaa aaatgcttat gaatcagcta
     2881 tatcacctta accatttgtt aatgagatgg gagaaaacat taaccacgat tctaacgcaa
     2941 acatcaagca cataaaacat ttaacactcc tcctttctgg ctgaagattt caaccaaaca
     3001 ggaagacagc gtatcaatta tagcatacac tttttcttaa attcattttt catatgacaa
     3061 aatatttcct tctaatcgac aaagttagaa ggatttttta ttgtctaaat acacaaataa
     3121 ccttaatttt taaagagcat aaggaaacgt gatatcactt tctcacgaaa ggtgttaggt
     3181 aggctgtgcc taccacgttt ctaagaaaga taaatgatat caaaattttg gatttgtgtg
     3241 tagatttgtg ggtaggatga aagcgaatgt gggcagtaaa ataagcgttt gtgtgtagca
     3301 aaatcaaaaa atgtgtaggg atatgtgtag ctacacatat ccctacacat aataaatttg
     3361 gaggaaaata tggacttatt aattagaaat attgaagaaa agtatattaa taaaattgac
     3421 aaacgttgtg aagaacttac ggtcaagact ggcaaaaaat ggagtcgaaa tcaataccta
     3481 aaagttttga tcgaaaatga ttttgatcac gctcttctga attataaaaa agaccaattt
     3541 gatcggttgt tagaaaaatt tgtggatatt caaacttata atacgaaaac tttagaagaa
     3601 tatattgcaa caaataatca attaattggt ttgttaattg aataataaaa atagtattgt
     3661 gaatttataa aaggaggaa
//
LOCUS       DACDHJ010000201         3674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDHJ010000201 DACDHJ010000000
VERSION     DACDHJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.205"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            119..1108
                     /gene="galE"
                     /locus_tag="IV223_002312"
     CDS             119..1108
                     /gene="galE"
                     /locus_tag="IV223_002312"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8712567.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1120..2604
                     /gene="galT"
                     /locus_tag="IV223_002313"
     CDS             1120..2604
                     /gene="galT"
                     /locus_tag="IV223_002313"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8712568.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2628..3608
                     /locus_tag="IV223_002314"
     CDS             2628..3608
                     /locus_tag="IV223_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8712569.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1241 a          663 c          806 g          964 t
ORIGIN      
        1 tttaccatat gaatttacga aattttactt tgtttgtttt caccataaaa gtaaacttcg
       61 ttttttaaat tatatagaat caacaaacac aattttacta atcaaaggag tgttttttat
      121 gacaatttta gtactaggcg gagccggtta tatcggttcg cacgctgtag accagctagt
      181 tcaaaagggg tatcaagtag ctgtggtaga taatttgctc acaggtcaca aacaagcagt
      241 acatccagat gctcattttt atgaaggaga catccgggat aaagaatttc tacgttccgt
      301 atttgaaaag gaaccgattg aaggagtaat ccattttgct gcaagctctt tagtaggaga
      361 atctgtcgaa aaaccattga tgtatttcaa caacaacgta tacggtatgc aaatcttact
      421 tgaagtcatg cacgagttca acgtgaataa aatcgtattc tcttctactg cagcaactta
      481 tggagagccg aaagagtctc caatcacaga agatacacca gctaatccta aaaatccgta
      541 tggtgaaagt aaattgatga tggaaaaaat gatgaagtgg tgtgaccaag catatggcat
      601 gcgctatgtc gctttgcgct acttcaatgt agccggagca aaagcagatg catctatcgg
      661 agaagatcat acaccagaaa cgcatcttgt cccaatcatt ttacaagtag ctcttggtca
      721 aagaaaagcc ttggctgttt atggagacga ttatgataca ccagatggaa catgtatcag
      781 agattatgtt caagtagaag atttgattgc tgcacatatt ttagctttgg aatacttgaa
      841 agaaggaaat gaaagcaact tcttcaattt aggcagcagc aaaggatact cagtcaagga
      901 aatgctagaa gcagctagag aagtgacagg aaaagaaatt ccagcagaaa tagcaccaag
      961 aagagctgga gatcctagcc gattagtcgc ttcaagtgaa aaagcgagag aggttctggg
     1021 ttggaaacct gaatacacag atatcaaagc aattatcaaa acagcatggg attggcatgt
     1081 cagccatcca aatggttatg aagaataggg gaatgagata tgtcaatcag tcaaacgatt
     1141 tgtgattttg ccacacttgc gatcgaagct ggcggatgga tggaaatgga ccgcttatat
     1201 ttacagaatc gtcttttagg gatggtagga gaagatgctc tagaaagcat tgaagtaaga
     1261 ccagttaagg aagaatctgt agacttattg gatcagcttg tcgctaaggc aagagaaaat
     1321 ggtgttcttt cagaattgcc agcagaacaa gagatttttg aagcgcaatt aatggatttt
     1381 ctcacaccgc caccttcagt cgtcaatgca ttctttgcac agcactattc gaaaagccca
     1441 gaggaagcaa ctgagtattt ctttaattta agtaagaaaa atgactacat caaaacaaga
     1501 gccatcgcaa aaaacatcaa atttccggca gagtcggagt atggtgaact ggaaatcaca
     1561 atcaatttat ctaagccaga aaaagatcca aaacaaatcg cagcagaacg cgaggcggta
     1621 aaagtagact atcctacctg catgctttgc atggaaaacg aaggatatag aggtcgcttg
     1681 aactatccag cccgcaccaa tcaccggatt attcgaatga atctagacgg tgaaagctgg
     1741 ggattccaat attctcctta tgcttattac aatgaacatt cgatcatttt atcagaacaa
     1801 caccgcccga tgaaaatcac aaaggaaaca tttgctagac tgcttcggat tactgaagtc
     1861 ttgcctcatt actttgttgg ttcgaatgct gacttgccga ttgttggagg atcgattctt
     1921 tctcatgatc attatcaggc aggacgtcat gaatttccta tggcaaaagc accgatagaa
     1981 aaacttatca aactagcaga ttatccgcaa gtcatcgcag gagtcgtgaa atggccgatg
     2041 tctgtgatcc gtttgcaatc aacagagaaa aaattgttga ttgaagcagc taactcagta
     2101 ttggaaaagt ggaaagcata ttcggatgaa acagtatcta tcagagcttt ttcagcagat
     2161 ggtacaccac accatacgat tacgccaatt gcacgaagaa gggacaatta ctttgagttg
     2221 gaccttgtcc ttcgagacaa caatgtttca gaagaacatc ctgatgggat ttttcatcca
     2281 cataaagatg tccaacatat caaaaaggag aacattgggt tgatcgaagt aatggggctt
     2341 gctgttttac cacctcgttt gaaaccagaa ctagtagaag tagagcattt cttattagga
     2401 gaagaaaacc agatggctga atatcatcgt ccgtgggcag aacaattaaa aaaagaacat
     2461 cctgatttga cgaaagatac ggtcaccgat gtcgtacaaa aatctgtagg tcaggttttt
     2521 gcacgtgtac tggaagacgc tggagtattc aagcgagatg aaaaaggaca ggcagcgttt
     2581 gaacgattta ctgcaatttt ataaacgtaa aaaaggagga gagacaaatg gcaacaatta
     2641 aagacattgc gcagttggca ggagtttctc ctgctactgt ttcaagagtt ctcaactatg
     2701 atcctgatct ttccgttggc ttagccacaa agcaaaaaat ttttgaagca gcagaacgct
     2761 taaattatac gaaacatcgt aaaaatgcag caaatactaa gcaaacgctt cgcctgatcc
     2821 agtggtacga cgatacggaa gagcttgaag atctttatta tttatctatt cgtctaggaa
     2881 tcgaaaaaaa ggcagaagaa gcaaacgtta ctttattgaa agaaacgtgg gattccatta
     2941 gtgatcttcc gacagatgga acgatcgcac taggaaaatt cgacgaagct cagttattag
     3001 aaatgaaagg atcccaaaat gccttgcttt tggtagattc tgacggcact tctcaaggaa
     3061 tcgactctct agttgtcgac tttaagtcaa gtgttcaaaa agtcattgaa cattttgtct
     3121 cccataaagc gcaacatatc gctatgcttg ctggaacaga atatacgaag aaaaaccatc
     3181 agcggctaaa agatcccaga attatggcat ttcaacaagt tttggcagaa aaaggctacg
     3241 acgaaggtcc aattatcgaa gcagattttt ctgtcgagtc aggatatcaa gcggtgaagc
     3301 agtatttaaa aacgaatccg catgttcctg atgcgctttt tgcagctaat gatgcattag
     3361 cgattggcgc actgaaagcg attcaagaaa aaggtttagt ggtaccagaa gatatttctg
     3421 tgatgggttt caatgatgtc agtgtagcga aatatgtaac gccctctctt tctactgtaa
     3481 aagtttatac cgaatggatg ggagagctag cagtggagac gattcttcaa ttaagtaatt
     3541 ccaaagcacc ggtttcaaga aagattattg tggaaacaga gttgattatt cgagaatcaa
     3601 cgaaataaaa gatggaaaag ctgtggggaa atgaagtgat accgcagctt ttttgtgttt
     3661 caggaagggg caaa
//
LOCUS       DACDHJ010000202         3601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDHJ010000202 DACDHJ010000000
VERSION     DACDHJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.206"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(100..1035)
                     /gene="murB"
                     /locus_tag="IV223_002315"
     CDS             complement(100..1035)
                     /gene="murB"
                     /locus_tag="IV223_002315"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8712570.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1108..2028)
                     /locus_tag="IV223_002316"
     CDS             complement(1108..2028)
                     /locus_tag="IV223_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8712571.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2141..2893
                     /gene="xth"
                     /locus_tag="IV223_002317"
     CDS             2141..2893
                     /gene="xth"
                     /locus_tag="IV223_002317"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8712572.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT         1039 a          715 c          636 g         1211 t
ORIGIN      
        1 taaaatgtac aagatttgcg ccatttgcgt aaacaaccac aaaaaagagc aggacggcca
       61 ccgtcctgct cttttactta ttttaagttc cattacttat taattggcta aacttctgct
      121 tgtttctgat attgcttgtt tttctccaat gatccgcact tctgtttcta aagacacatc
      181 aaatttttct ttgattactt cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt
      241 cgcatgatcg atattgacga tgaatccagc atgtttttca gagacttgtg caccgcccca
      301 tttcaggcct tgcaatcctg cttgttggat cagctgacct gtatagtgtc ctactgggcg
      361 cttaaagaca cttccgcatg aagggtattc taatggctgt tttgattgac gcaattcggt
      421 caactcatcc attcttgctt tgattgcttc ataatcccct gtttgcaaac tgaatcgagc
      481 ttccaatacg atagcacgta attcttggac tttactgtga cgataagaaa ataccatttc
      541 gtcttttgtc aaagttttca atgttccatc ttcaagcaaa agatccactt cagcaaaaac
      601 atctttgatc tctccgccat acgcacctgc attcatataa accgctccac cgacacttcc
      661 cgggatgccg caagcaaatt caaatccagt caatgattct gccaaagctg cataagtcgt
      721 atcaatcagt gaggcacctg cttccgcaat gaccatcgtt cctttgacat gaatctgttt
      781 catttcagtc agcatgatga ctacaccgcg aatcccgcca tcttgaacga tcagattgct
      841 tgcatttcct aataccatcc aaggtatatc atggattcgg caataatcga tgatttgctt
      901 tacttcttct tgttttttcg ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa
      961 cgtatagttc atcaatggtt catcaaacaa tatagtaatc gtggctaagc tttcaactat
     1021 ttcttcttta ttcactgagg attttccttt cttttctcaa acgtcctcat tattctatca
     1081 cgcttaatac aatcttgcca gtcttgttca aagttgatga cggtagtgat ctttaagcac
     1141 ttcctcacat aattcatgac ttcttacaac ttctttttgt ttaagcaatt ctgtttccga
     1201 tttacctaaa cggatctgag aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac
     1261 tagcgtgttt gtccagtcgc caaattcttt cacttttttt ccatcagcat cttgaatgac
     1321 aagctgggtc agatcgttta cttcataagt accattttta cttgttactt gatatttttc
     1381 tatattagcg ccacttgcta aatccatcgt taaaatagcc gtttgatttt gtgtttctaa
     1441 ttgtaaaatg gcatattcca aaaattcctt tgtttctctt atctggtatt ttgttctcaa
     1501 aatgggttca tctaataaat acacagctgt atcaataaga tgtaaaaaca aatcataaag
     1561 gacgaactct gtcgtttctt ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt
     1621 atctgggata cgtttcaatt cttctaccat aggagcaaag cgccggttga aaccgatcat
     1681 caaaattatt tggttcttat cagctaattc ttgcaattca cgaacttcat ttagatggac
     1741 actcagtggt ttgtcaatat aaacatggat accattttct agacattttt tggctaattc
     1801 ataatgtact tttgttgctg cgtggatcat acatgcttcg attttttctt ctaaaagctc
     1861 atcgatcgtt tcatatagat gttggaaccc gtactctttt tgaattcttt cttttgtttc
     1921 tttatttctt gttgcaaaat aaaaatcaag tgttccttgt tttttcgcat aagttggtaa
     1981 atatgctttt tctgcgatat tccctacacc gattacacca atcttcatct actttcctcc
     2041 tggatcattt agttactttc atcttacacc attttttact aaaatgaggt acaatagtat
     2101 cgcaaaggta aggaaattaa ttcgaaggag tgtgctttta ttgaaattta tctcttggaa
     2161 cgtaaacggg cttcgtgcca tcgtaaataa aaatttttta gaagtttttc atgagttaga
     2221 cgccgatttt ttctgtctgc aggaaacgaa actccaagca ggacaaattg atttagatct
     2281 tccaggttat tatcaatatt ggaattacgc ggaacgaaaa ggctattccg gcacagcgat
     2341 ttttgctaaa aaaccagcat tgaatgccac ttacggaatg ggtatcgata tccatgatac
     2401 agaaggccgt ctgattactt tggaatattc cgatttcttt cttgtaacct gttacacgcc
     2461 aaattctcaa agcgaattaa aacgtttaga ttaccgatta gaatgggaag aagcctttta
     2521 taattatttg gaaaatctaa aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt
     2581 cgctcatcaa aagattgatt tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac
     2641 tccagaagaa agagcggctc tttctcgact gttggataac gggtttatcg atacattccg
     2701 ttatttttat ccaacccagg aaggtgtcta ctcttggtgg aactaccgat tcaattcaag
     2761 gaaaaacaat gctggttggc ggatcgatta ctttttgacg agcaaagatt tagagccagg
     2821 actagctgat gcaaagatcc atacggacat tatgggcagt gatcattgtc cagtagaatt
     2881 agatttgaaa taaagaggtt gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt
     2941 gtgaaaatgg ctttccacat tttgcataaa tttggcataa taccgagaaa ctggcttttg
     3001 aacactgttt atcgagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
     3061 aacaaaaata gttcctcata tttttcgttt ggttgggctt attaccgcag atacaagctt
     3121 ttgaacaccg tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag
     3181 ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg cagatgcaag
     3241 cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa gtaataagaa
     3301 cagccaaaca aaaatagttc ctcatatttt tcgtttggtt ggacttatta ccgcagatgc
     3361 aagcttttga acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag
     3421 agaaacaaat ggcaaaatag ttcctcatat tttgctattt tttgagctaa taccgagggt
     3481 cagctgattg aacaccgttt agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt
     3541 aagaaatttt tttgaaaatg gttcctcaca actttgcata aatttgactt aataccgaga
     3601 a
//
LOCUS       DACDHJ010000203         3586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDHJ010000203 DACDHJ010000000
VERSION     DACDHJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.207"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..731
                     /locus_tag="IV223_002318"
     CDS             51..731
                     /locus_tag="IV223_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8712573.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
     gene            859..1818
                     /locus_tag="IV223_002319"
     CDS             859..1818
                     /locus_tag="IV223_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAP8712574.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(2251..2823)
                     /locus_tag="IV223_002320"
     CDS             complement(2251..2823)
                     /locus_tag="IV223_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8712575.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2839..3444)
                     /locus_tag="IV223_002321"
     CDS             complement(2839..3444)
                     /locus_tag="IV223_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8712576.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1159 a          707 c          621 g         1099 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat accgtaaggg attttattct ttacgccgat tgtaaaatta
      781 agctagacaa ataaataagc cattcgtgct acactcaaaa tgtaattccg actaaagaaa
      841 acataggagt gatcacgaat gacctacacc catcttacca cggatgaact ggtgatgata
      901 gagtcttact accaccaaaa catcaaaatt gtgaaaattg ctgagtacct gaaacgatct
      961 cgaacaccga tttataacgt cattaatttc ttgaaagaag gccatacggc cctcgaatac
     1021 taccaacagt ataaagaaaa taagaaacgg tgtggcagac gaaacatcgt cttgccaaag
     1081 aaacaacagt cctacattaa agaaaaaatt gcccaaggct ggacgccaga cgttatcatt
     1141 gggcgtaaag agaaaccaat cagctgttcc gtaagaaccc tttatcgcca gttcaaggaa
     1201 aaggtgttcg atgaatcgac tttgcccatg aagggtaagc ggaaaccaaa tggacatcaa
     1261 gaacgccgag gaaaacaggc ttttaaacgg aatatcgttg aaagagaaac tgattacccg
     1321 acatttaaag aagagttcgg ccatatcgaa ggcgatacca ttgtaggcat tcatcataag
     1381 agtgctgtca taactttggt agaacgtcta tcgaaaatca tcatcacctt gaagcccgag
     1441 gggcgcaagg cgatcgatat tgagaatact ctgaaccact ggtttggatc gattcccaga
     1501 catttattta aatccattac ctttgattgt gggaaagaat tctctaattg gaaggcttta
     1561 agtaaccaac atgacatcgc tatttatttc gctgatccag ggactccttc gcaacgagca
     1621 ctgaatgaaa attccaatgg cttgctgcga aaagatggtc tgccaaaaaa catggatttt
     1681 aatcaagtcg accaagcttt tatctcttca gtggcgaata agagaaacaa tattccacga
     1741 aagtccttgg attatcaaac acccctggaa gtatttatga gttacatgaa tgaagatatt
     1801 ttgtctagct taatttgaca aatcaaatta tttaaaactt tgcaacagaa ccttagcagc
     1861 agtgctgatt tttatgatag ttatttggtt cttaaatgaa agatctaaag acaatcaaca
     1921 ccgtagggat atggagataa aatcaataga gtcaatagat tatttatcga taataacact
     1981 aaaaaagcca cgttcctaaa cgtgacttac gctgaacgat atgtgttgtt tggtagacga
     2041 acaaatcgtt cattataaat atatgatggt atcctaatat agtaagcgtg gctagcatac
     2101 ctttaattac tttacctttt acagttacca tacacatttt ttgagcagaa atcaagtaaa
     2161 taaaaaaata ttatatttca caacatgcta atagactcat atctagaacg cattaagtat
     2221 ttacctgtct ggttcgctga gacttctttt ttaactttct tcaattttct tctttattct
     2281 aaaaagcgta ctgcgactga aaccggttat agcttcaact tccttatacg atttacctga
     2341 agtaagcagt tcatatgcgt gcctcttctt cggtgtgatt gttgctttcg gacgtccttc
     2401 tctgaaatta ggattattct ttcttgcaaa catttttcct tcttgggtac gttccacaat
     2461 catatcacgt tccatttctg ctacactcaa caacgtccgt gtcaccattc gccccatgga
     2521 cgtattttca atcgttccta gattcaaaac cttgatcgtt acgttatcat cgagtaaagg
     2581 ttcaatgatc tctaatgctt ctctcgtgtt acgagcaaat cgatcaagct tcgtaacaac
     2641 tatttcatct ccaggcttca gaccagccaa cagctgattg aattttggcc gatcccgttt
     2701 tgtacccgta aatttttctg aaaaaatatt cttcttctct acgccgttct caagcaactg
     2761 ttgaatttgt tcttttgtac tttgagaagc ggttgagaca cgtgcatatc catatttcgt
     2821 catttggtcc acctactttt acaaatcatc tatcgtatac tcagaatacc cttcatagaa
     2881 atagctgaca tcgattcctt tcatatataa ctcgcgatcg tcaattttat cagtcagagc
     2941 attggaaatt agatagcgta tttccaaatc gtttatggga cttcgctcca ttgcagaaag
     3001 atagtctgct ttatcgatta agttccaatc tacaactttt tgcaattctt cctttaaaat
     3061 gagatccagc caaattctag tacttcggcc atttccttct cgaaatgggt gagcgatatt
     3121 catctccaca tatttcttga taatctcttc aaaagaagtt tgtggcatct gatcaatatg
     3181 ctgtaacgaa tgttctaaat acataactgg tgcaaatcga aaatttcctt ttgcaatatt
     3241 gaccgatcgg atttttccgg caaaatcgta aatatccgaa aatagaaatt catgaatttc
     3301 agataaacct ttaaatgtac caacttcgat ttcatttatt tttccacaat cgtacaattc
     3361 tttcgctttt tgtttactga gtttttcttc aaccttagcc aactcaactt gattggttaa
     3421 tcccaattta ttctctaaaa tcatgcgaaa tctccttaac tttatgacat aagtttatga
     3481 aacactctca accccattat atcgcgattt tgtttatgtg tcaaaaacta ttaagtttct
     3541 gacccccgat agatttttca agtgtctcta ttttgcacag tctagt
//
LOCUS       DACDHJ010000204         3581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDHJ010000204 DACDHJ010000000
VERSION     DACDHJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.208"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1367
                     /locus_tag="IV223_002322"
     CDS             <1..1367
                     /locus_tag="IV223_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8712577.1"
                     /translation="ARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNLETFQHSAII
                     ISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPRLVIL
                     LSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKHKVEA
                     VILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENLTLIN
                     PNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVKVTSP
                     GPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRAVRFD
                     ELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKYVS
                     DYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIAFPNE
                     KIFR"
     gene            1383..2423
                     /locus_tag="IV223_002323"
     CDS             1383..2423
                     /locus_tag="IV223_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8712578.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2442..3527
                     /locus_tag="IV223_002324"
     CDS             2442..3527
                     /locus_tag="IV223_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8712579.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         1276 a          521 c          666 g         1118 t
ORIGIN      
        1 ctgcacgacg gatattcatt atattaatgg atgttattgt atacaatgca gcaatatata
       61 gtagtttctt actgaagttt cgtggagaga tcccattacg aaatttagaa actttccaac
      121 actcagcaat tattatttca gtgattttta ttgcgttgaa cattttgttg ggtgcatatg
      181 ttttttataa tcgaatgatc agtgacatcg tgtttgtcac agtcatcggt caggtattga
      241 tgacactagg gatcatggta gtgacttttg cgggccgctg gtttgctttt ccacgtcttg
      301 ttatcttact ttcatttgtt ctcggtacgc ttttcttaag tatctatcgt attctcgtat
      361 ataaacttta tttacgcgta agtcgagata aaaaggtcgt cattattggg ttagaaaagg
      421 atgtcgcacc agcaatccaa aattttggtt caaagaaaaa caacaaacat aaagtcgaag
      481 cagtcattct tgataactat tatgcaaaca tcaaaaatat gattaaagac atcgatattg
      541 tctatcttgc ttctcatatc gaagaaactg aaaaaatcaa aatctatcaa ttagttacta
      601 aaaatgaaaa aaaattgttt ttgaatacaa catttgaaaa cttgacattg atcaatccaa
      661 atattatgaa tttcgaagat gaaagtatta ttgaagcatc tggatttaga atccaacctg
      721 aatatgaact attcaaacgt ttgtttgata tcatcgtttc acttgtttta ttagttattg
      781 cttcaccatt tatgttattc acagcaattt tagtcaaagt gacatcaccg ggaccaatca
      841 tctataaaca agtgcggatc acaaaaaatc aaaaggaatt ttcaatctat aaattccgct
      901 cgatgaccgc cacagccgaa gcaaaatctg gaccagtttt agctaagagc aatgatgcac
      961 gagtaacgcc tgtaggtaaa tttattcgag ctgtccgctt tgatgaatta ccacaaatct
     1021 tcaatgtgct gaaaggcgat atgtcgatcg taggaccacg tcctgaacgt cctttcttcg
     1081 tcgatcagtt caatgcagaa aacccttatt actacttacg gcataatgtt cgtgctggta
     1141 tcactgggta tgcacaagtc tatggaaaat atgtctctga ttacaacagt aaattacgtt
     1201 ttgatttact ttatatcaaa aaatattcat tgatgcttga tttaaagatc ttgctgcaaa
     1261 ccatcaagat cctatttgat aaaatgtcct ctcaaggact tgaagaagac gaaaatgtga
     1321 tctcattggc agaaattgct tttccgaatg aaaaaatctt tcgctagttg ggaggcgtaa
     1381 aaatgaacaa tccgaaattc agcataatcg taccagtata caatgcagaa gcaactattg
     1441 gtcgaacgat tgatattcta aaagaattgg attatgaaga gtacgaagtc gttttagtaa
     1501 atgatggatc aacagatcaa acacaggaaa tcattcaaga actgattgaa aatgatgaac
     1561 gttttgaatt gattactacg accaatcaag ggcccggatt agcaagaaac gaaggaataa
     1621 gacagtcaaa aggagaatat ctcttattct tcgatgcaga tgacacgcca gtaaaaacga
     1681 tcttgtcaga ttatgatcat ttgctagaaa agagtccaaa agcggatctt gttgttagtt
     1741 cgtttgtttt tcgtacaatg gacgagggaa gaatcgtttc cgaaaaagaa aacttagtta
     1801 cagaatgtca ctatctaact cacgaagagt ttataaaaga catgtatcag ttaatgaatg
     1861 aacagttgat gtatgttgta tggaacaaat gctatcgtag cgatattctg aaaaataacc
     1921 aaattctatt taaaacttat aatagttgtg aggatcgaat atttaactta cattattatg
     1981 aatactgtca tcaagtgatc atgaacccaa aagtagaata tatttatgaa tttgaaggtg
     2041 ggaaggggat cactaatcaa taccgaccta acaagttcga aacatttaaa gaattctatt
     2101 cattagcaaa tactgtaaca gacgatatta ataaagacgg tatggcggcg cttcttttaa
     2161 aagggacaac ttcagttatt ttttctattt atagtacctc tgcaagaact tcaaaagaga
     2221 agaaagacga agcaaagcag atattgagtg atccagtgat ccgagaagcc aagcaaattg
     2281 ctaagacgga ttcttcagca aaaaaaataa caaagttgct ttacaatatg ccagctccac
     2341 tatttttatc tgctgtaaaa gttggaagct tcgttgaagt gaaaatgcca gggttaatgg
     2401 ctgctttgaa gcgagtatat tagttaatgg aggaaaatat aatgaaaaga attttgcatt
     2461 ttcagggacg aatgggttta ggtggagcag agtctttcat gatgaattta tatcgtaaaa
     2521 ttgatcgtac aaactatcaa tttgattttt tgatttatga agattatgca gatgttcaag
     2581 actatcattc agagattgaa agattaggag gccgtatttt tgttgtacct aatcctaaga
     2641 aaaatatttt taaatattta atagaagtga ataagttact aaaaaaagaa tctttttcta
     2701 tagttcacaa tcaggtttat tttggcgggg gaattaattt atggttagcc aaaaagaatg
     2761 gaatcagaca gagaattgct catagccatg caactgaaga tggaaaatct caaaatattg
     2821 taatgaatgt attgcgtaaa tttttaacca aattgttgtt acaaaatgca acagactacc
     2881 ttgccgttag tcaagaagct ggtgaaagtc tgtttcaaaa ccatcccttt gagatagttc
     2941 ataatggtat tgatttagag ctttatagta aaaattcaga agctaaggtt aataagagaa
     3001 aagaattgga tatctcaatg agtacttttg ttgtgggaaa cattggacga ttagagaaac
     3061 aaaaaaatca atcttatttg ttagagattt ttttagaaat actaaaaatg aattctgatt
     3121 cttacttgat tttagtaggt gaagggtcac ttagaaataa actgaaagcc aaagcagtag
     3181 aattggatat agagaaaaga gtaaaatttt taggagaaag aaacgatatt cccgagttat
     3241 tatctgtgat ggatgttttt gtaatgcctt ctctttatga aggtctgcca atggttggat
     3301 tagaagcaca agcagcaaaa ttaaaattag tcttagcaga tactatttcc aaagatacaa
     3361 agttaacaaa taatgtggat tttgtatcat tagatgaaac accacaagaa tgggcaaaaa
     3421 aagtaataaa agagccgtat gctaataaaa taacacaaga actaaagagt tatgatgttt
     3481 cgtacactac tgaacaaatg gagagtatat atagtagaga tcattaaaaa tgttcaagat
     3541 attttaggag tgattatttt tgaaaatatg ttatttgata t
//
LOCUS       DACDHJ010000205         3572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDHJ010000205 DACDHJ010000000
VERSION     DACDHJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.209"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            271..465
                     /locus_tag="IV223_002325"
     CDS             271..465
                     /locus_tag="IV223_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712580.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            455..808
                     /gene="tnpB"
                     /locus_tag="IV223_002326"
     CDS             455..808
                     /gene="tnpB"
                     /locus_tag="IV223_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8712581.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            910..2457
                     /locus_tag="IV223_002327"
     CDS             910..2457
                     /locus_tag="IV223_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8712582.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKTDKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            2534..3572
                     /locus_tag="IV223_002328"
                     /pseudo
     CDS             2534..3572
                     /locus_tag="IV223_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1217 a          624 c          711 g         1020 t
ORIGIN      
        1 gtaaatagaa atttagggta gaaaaaaaag tccattctgt aaaattaaag ttaccacaca
       61 gaaattttat aggaggactt tctcatgact aaatttacta tagaaattat gcaaacacta
      121 ctcaataaag gggatttaga tgaattattt caggatcatt tagagcgagc gattaattca
      181 cttctacagt aagcgcccca tagaacagta ccttactaac aaatgcacct caagatatac
      241 ttgagctatc aagaagtctg gaggttttct atgtataaat taaatgagat tgttcctgtc
      301 caattaggaa acgaaaagag tgcaccagaa aaaggtaaac ccgcggctgc caagttagcg
      361 atgcgcatca aaagagagaa tggttctgaa gttttgatct ataacggtat taacaattat
      421 attcttcaag ctattttgaa agaactgttg tccaatgctt tatgatttac ttcaaaccaa
      481 acatatttat attgtgtgcg gaaaaacaga tttgcgaaaa ggaattgatg gtcttgccag
      541 tcttattcaa caagagtatc aactcgagct ttacgaagat gccgtttttt tattttgcgg
      601 gaaccgtcaa gaccgtttca aacttctcta ttgggacggt gatggttttc ttctctgtta
      661 taagcggata gaaaacggca agttaaaatg gcctcggacg aaagacgaag ttcgaacctt
      721 aacgaaccaa caggttcgat ggttgttaga aggattaagt atcgatcaac ctcgagctat
      781 tttgccagga aaaaaaggag ttttttaagc tggggggtta acacaaaaaa tgtgttgaac
      841 ccctatttct tgtgcgtttc ttgtggtatt ctgtaacaaa atcagttcga ttcaaggtgg
      901 tgaaaaattt tggaaactac agatacgttg cttcagttgc ttcaagaagc ccataaaacc
      961 aatcaagcac aacaacaaac cattcagaat cttacaactg aaatccaact attgaatgaa
     1021 aaggtcaatt atttaaccaa caaattattt ggacgctcta aagagactct ctttgaagaa
     1081 acgaatgggc aattgaatct ttttagtgat gaagaaattt ctgtaagtgt tccagaagca
     1141 gctgccacca ttattccagt aaaaggacat caacgagttg taggaacgaa aacagacaaa
     1201 atcaaacact tacctataac agagaaagaa catctccttc ctttggaaga acaattttgt
     1261 gaacattgcg gttcacagat gaaagatatc ggacggacaa aagtacgaga agaaattcgt
     1321 ttccatcagg ctatgttgga ttgtctcacc cattatcaac atacctattg ttgtaaaagc
     1381 tgcgaaaaag aaggactttc ttcttttaaa aaagcgatcg ttcctaaacc tttgatttct
     1441 aatagcttag gttccaatag tttagtggcg gaaacgatcc ggatgaaatt tggtcagaaa
     1501 gttcctgcct atcggcaaga gaactattgg aaacaaacac atgggttgga tatttcgaga
     1561 gataatatca ccaattggca tattaaagca gtgcaaaacg cgttagaccc tttaggagaa
     1621 cgattaagag tctatcttaa tcaagaagag attctacacg gggatgaaac aagttatcgt
     1681 gtgattgaaa gtgccaaaac agatacctat tactggcagt tttgtacagg aaaagacagt
     1741 cagcatccca tcgtttatta tcaccacgac gaaagtcgtg ctggagatgt tccgaaaact
     1801 tttctcaagg agtttacagg gtatttacac tgtgacggct atagcgggta taatgccgtg
     1861 gaaagtgtac gacttgtcta ttgtttcgcg catgttcggc ggaaattttt cgaagcaatt
     1921 ccaaaaggaa agaaaaacac ggatattccc gcagctcaag cagtcaaaca attggataaa
     1981 tggtttgtcc tagaaaagaa atggaaagac ttttctcctg agaagcgatt aagctgtcgg
     2041 caacaagaac tgcgtccatt atttattgct ttttatgagt ggatggcaac gatagatcct
     2101 gttgcaaaat ccaagttgga tgcagccgtt caatatgcgt gtaaactacg aagtggtttt
     2161 gaacctattt ttgaagatgg tcgtttagaa ctcacaaata atcgtgcgga gcgaaatatc
     2221 aaagagctgg tgataggacg gaaaaattgg ttgcattcca caagtcttga aggagcacga
     2281 acttcaggaa tcattctgag tgtatataaa acagcagaac ttaacggatt gaatcctgta
     2341 aagtatctag agtttctatt tgataagata ccaaatcttc ccgtactttc agcagaaact
     2401 ttagatcaac tgttgccttg gaacaaggat gtacaacaac atttttctcg taactaaaaa
     2461 cgccaaacat tcccgattct attctatagg tcgggttatt tgacgtacag gtactgttct
     2521 atggggcgct taccttctac aagcagagct aactgctttt ttagattacg aaaaatatga
     2581 tcgaaatgga ttcaattcag gaaattcccg taacgggaat tattcacgta catttaaaac
     2641 agaatatggc gagttgaatt taactattcc tagagatcgg aatggcgaat tttctcaaca
     2701 aacactacca gcatataagc gaaccaatga ttcattagaa acgaccatta tccaattatt
     2761 tcaaaaaggc attactatgg ctgaaatctc caaattaatt gaaaaaatgt atggtcacca
     2821 ctatacgcca caaacaatct ccaacatgag taaattggtg gctgaagatg ttttagcttt
     2881 taaagaaaga acgttagaag ccaattattc cgttatattt atggatgcga cgcatatccc
     2941 tgtgaagcga cagactgttt cgaaggaagc agtgtatatt acgattggta ttcgtttgga
     3001 tggaaccaaa gaagttcttg ggtttactat tgccccaact gagtctgctt atatttggaa
     3061 agaagttctt caagatctta gaaaacgtgg gttagaagaa gttttattag tagtgacaga
     3121 cggattaagc tgtattgaag aaagtatcca tagtgtgtat ccgaatgccc aatttcaaca
     3181 atgttgtgtg catgtatcta gaaatatcgc tcataaagtt cgtgttcgag gtcgaaaaga
     3241 aatttgtgag gatttcaaat tggtttacca agcgaattca aaagaagagg cattggatca
     3301 catcgacttt atgactagga aatggaaaaa gcagtatcca agagtcgtca atttactctt
     3361 gaatcctgcc ctattaacct tttataattt ccctcacgcc atcagacgaa caatttattc
     3421 gacgaacctg attgaaggct ttaataagca gctaaaacga tatactcgaa gaaaagaaca
     3481 attccctaat gaagaatctc tagagagatt cttcatttct caatttaatc aatataacca
     3541 aaaattttta ggtagaattc acaagggatt ta
//
LOCUS       DACDHJ010000206         3498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDHJ010000206 DACDHJ010000000
VERSION     DACDHJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.210"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..341
                     /locus_tag="IV223_002329"
     CDS             <1..341
                     /locus_tag="IV223_002329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712583.1"
                     /translation="SVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWGKAP
                     QKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFHNLI
                     DGVLSAASRY"
     gene            complement(542..817)
                     /locus_tag="IV223_002330"
     CDS             complement(542..817)
                     /locus_tag="IV223_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8712584.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            925..1689
                     /locus_tag="IV223_002331"
     CDS             925..1689
                     /locus_tag="IV223_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8712585.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1812..2315
                     /locus_tag="IV223_002332"
     CDS             1812..2315
                     /locus_tag="IV223_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8712586.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2723..3370
                     /locus_tag="IV223_002333"
     CDS             2723..3370
                     /locus_tag="IV223_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8712587.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         1168 a          626 c          703 g         1001 t
ORIGIN      
        1 caagtgtttc tttcttattt aaaaaactaa aaactttttt tattctgccc gaaatagagg
       61 agtcaaatga actcactgca agtgatagat cagctttttt actacctgtc tttaatactt
      121 ggggcaaagc gcctcaaaaa caaccttatc gacggtgttc aaaagtcagt ttcttggtat
      181 taagacaaat ttatgcaaaa atgtggaaag ccattttcac aaaattttcc ttaatactca
      241 aaactaagcg actttttcac aaccagtcct taatgcttga tgcagaccag ctttttcaca
      301 accttattga cggtgtttta tcagctgcct ctcggtatta agtcattaca tttcaaaata
      361 tgagaagcta ttttgaaaat gtttctcctt aatactcgag gcaaagcgct tcaaaaacaa
      421 ccttatcgac ggtgttcaaa agtcagtttc tcggtattaa gtcaaattta tgcaaaaatg
      481 tggaaagcca ttttcacaaa attttcctta atactcaaaa ctaagcgacc ttttcacaac
      541 cttatcccac cacatcaaat ttctttcgtt cctcaatcat cggatcttct tggacatcca
      601 catactgcac tcgtccactt gggctcggag acttcttgac ttcttcgata aatttttgga
      661 tgatgtcgtc atttccgatt gcttcgattg tgactgagcc gtcgtcttcg ttttttaccg
      721 ttccggtcac gcctaactga tcagcaacca tcttggtcat atagcgaaat cccacgccct
      781 ggacacgtcc ttggacattc attttaactt tgcgcatgtt cgtcaccttt ctttcctgtt
      841 ttataaatca atgataccga tttttttagg atgaaacaag ctcaagcacc tattccatga
      901 tataatcagg tgaggtgaag gaatgtgaaa gagatccaat cagcaaaaaa tccaagaatc
      961 aaagaattaa aaaaactaca taaaaagaaa tacagagaag aaaaggcaga atatcttttg
     1021 gaagggttcc atctgatcga agaagctgcg tcgtctgatg cagaaataaa agaacttttt
     1081 gtgacacaga gaggtatgaa agaatgggga gactggctgg ctgtaaacaa agagatttct
     1141 acttttctag tttcagatga ggtcatgacc gctatgtcag atctacctac accgcaaggg
     1201 atcttagcag tgatgacgtt aacatctgaa gatacgccag atttttcagg aggctggctt
     1261 ttactagata atgtacagga tcctggaaat gtgggaacga tgatccgtac ggcagatgcg
     1321 tttggtatgg ctggtgtttt attaggtaaa ggaacggcag atatttatag tacaaaggtg
     1381 ctgcgaagca tgcagggaag caattatcat ttgcctgtgt tacaaaaaga tttggcagat
     1441 gtaatccctg taatgaaagg caaagggttt acggtgtacg gtacagaatt aaatgaagaa
     1501 gcacatgcat tagacaaagt agaaaaaaca gaggatttcg ctataatcat gggaaacgaa
     1561 ggtcagggag tcagtcagga aatcttgtct ttgactgatc agaacgtata tatcccaatg
     1621 aaaggaaatg cggaatcatt gaatgtcgga gtcgcagcag ggatcgttct ttatcatttt
     1681 tctttatgat taaggaacta ttaagcttta gctattttat ctagagcgac tagaataatc
     1741 cgaacactgg tggtatactt agatcaattt cagaagaaaa tgaaattcaa aaaggtggca
     1801 gatcgataat tatgacagaa aaatggcgtg aagacgaaga atatatgtct tatgtacaag
     1861 atcttttaga aacagaagca gtgaaaaaat tagcgaatta cacacagcat gttcattcca
     1921 ctcgtttgga gcattccatc agtgtttctt actatagtta tctgctcgca aaaaaatggg
     1981 gtggcaatgc taaagcaaca gcgcgtgctg gacttttgca tgatctgttc tactatgatt
     2041 ggcgtacgac gaaatttgac gaggggacac atgcgtatat ccatccaaga attgctgtaa
     2101 agaatgcaga aaaaatcacc gatttgtcgg atcttgagcg agatatcatt ttgaagcaca
     2161 tgtggggtgc aacgatcgca ccgcctaagt ataaagaagg atatattgtg acattcgtgg
     2221 acaaatactg cgcagtcaaa gaagcggctc agcccatgtc tgcaagtatg cgtaagagat
     2281 ggcaacgtta ctttgggaaa gaatcctcga tttaacaggt aaaaccaata atagaccata
     2341 ttgaggttgt gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattact
     2401 tctcaaattt ttgtgaagtt tcggcttatt actgcagatg caagcttttg aacaccattc
     2461 attcgaaaca agggactttg acacgacttt tgtcacagtc cttttttctg tttgcagttg
     2521 actatctgtt caagatacgg tttactttta tttatcgaat gaatcatgta catagaataa
     2581 taagcattaa agggggtgat gataagatgc aaaacgtatc ttatttaaaa gagtccagga
     2641 tcttcaggta acacatactt tgcagaagat cacttttttc tacaaagtat gcactttggt
     2701 tagaagaaag gactttaaaa tcatggaaca aacaataaaa ccataccaat atttttttat
     2761 aataagagaa gtagaacagt tattaaatgc gtactcatct gtcaatgatc caaaaacggt
     2821 acaaacagtt caggcactag cagctgaaaa gattcgagac atattagatc acgaactgcc
     2881 agaaatcgat acatttttaa cgacagtttt agacgtaaaa ctgacaaaag cgcaagcaga
     2941 aagagcattg gaggagctga agcaaatcgt tcagccgttt atgcagccta gcaaaccaca
     3001 aatcgagaaa ttattccgaa aagtgaaaaa actcaaacaa ccagcttgga atgaaatcga
     3061 cttgcaagaa catacgtaca tcggctggaa tgatccaggt acgcagaaaa aattcatggt
     3121 ctattacgaa gatgaaaaat taaaagggat tagcggtact ttatcaccaa cgataaataa
     3181 gggaatctgt gcaatctgtc agaaaacatc gaatgtttcg ctttttcttt ctacaacgaa
     3241 agcaggttct gatggcacgt atacgaaaaa ggggaattat atttgtcatg atagtgacca
     3301 atgtaatcaa cagttaacga aattggctcc tttctatgaa tttgctgaac gtgtaaagaa
     3361 agaacgataa ttttattgtt ttttacagaa aaatatgcta tcttttaatg gaagtgaata
     3421 ggtgatggtg ttcgccttta aatgctaacg aaagatagct aatgacgcct actaaagtct
     3481 ggggacagcc cctttctt
//
LOCUS       DACDHJ010000207         3364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDHJ010000207 DACDHJ010000000
VERSION     DACDHJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.211"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(507..869)
                     /locus_tag="IV223_002334"
     CDS             complement(507..869)
                     /locus_tag="IV223_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8712588.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1092..1844
                     /locus_tag="IV223_002335"
     CDS             1092..1844
                     /locus_tag="IV223_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8712589.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1984..2907)
                     /locus_tag="IV223_002336"
     CDS             complement(1984..2907)
                     /locus_tag="IV223_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8712590.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2922..3194)
                     /locus_tag="IV223_002337"
     CDS             complement(2922..3194)
                     /locus_tag="IV223_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712591.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1104 a          633 c          542 g         1085 t
ORIGIN      
        1 ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat
       61 tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa
      121 aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat
      181 aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt
      241 ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt
      301 ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca
      361 cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg
      421 gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt
      481 accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc
      541 actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc
      601 actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag
      661 tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca
      721 tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata
      781 gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta
      841 ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag
      901 atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac
      961 ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa
     1021 aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag
     1081 gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga
     1141 gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag
     1201 gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa
     1261 caatggcatt caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc
     1321 agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc
     1381 tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca
     1441 aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac
     1501 aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga
     1561 atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc
     1621 tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc
     1681 ataagaagtg gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag
     1741 gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg
     1801 gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca
     1861 acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt
     1921 tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata
     1981 tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa
     2041 caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag
     2101 aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata
     2161 gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag
     2221 ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac
     2281 aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt
     2341 ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt
     2401 caaataagta agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg
     2461 ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc
     2521 atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc
     2581 ttcaagatac cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta
     2641 atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt
     2701 ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt
     2761 tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc
     2821 atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag
     2881 ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct
     2941 tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc
     3001 gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc
     3061 attggtattt cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg
     3121 gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct
     3181 acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt
     3241 ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa
     3301 agtctcttat tttaaattga atacattata atgatactgt aaatatatct ttattattag
     3361 tgaa
//
LOCUS       DACDHJ010000208         3343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDHJ010000208 DACDHJ010000000
VERSION     DACDHJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.212"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..741
                     /locus_tag="IV223_002338"
     CDS             <1..741
                     /locus_tag="IV223_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8712592.1"
                     /translation="NSGKTIIYSTMSDIKKNTKVGDATIIRFCQKIGFSGFSDLKIEI
                     AKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFG
                     VGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYD
                     SLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLV
                     HGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            971..1201
                     /locus_tag="IV223_002339"
     CDS             971..1201
                     /locus_tag="IV223_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8712593.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1424..1522)
                     /locus_tag="IV223_002340"
                     /pseudo
     CDS             complement(1424..1522)
                     /locus_tag="IV223_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1787..2242)
                     /gene="tnpA"
                     /locus_tag="IV223_002341"
     CDS             complement(1787..2242)
                     /gene="tnpA"
                     /locus_tag="IV223_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8712594.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2485..3343
                     /locus_tag="IV223_002342"
                     /pseudo
     CDS             2485..3343
                     /locus_tag="IV223_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1105 a          579 c          583 g         1076 t
ORIGIN      
        1 aattctggga aaactattat ttatagtacg atgagtgata ttaagaaaaa cacaaaagtt
       61 ggcgatgcta ctattattcg cttttgtcaa aaaattggtt tttctggttt ttctgattta
      121 aaaattgaaa ttgcaaagga agactttagt caaaaaaaag aacatccgac tagtgtaaag
      181 tactatgatg aaattgctaa tagtttaaca gaagctctct attccactat taggctttta
      241 gacgaagaaa aattgaatga agccattaaa atgatcactc aatcaaaaaa catatatata
      301 tttggagtgg gatctagtgg aaatactagt cttgatttag aaaatatgtt tcttcgcgta
      361 ggagttcaag caaaagctgt actagaccct cattttcaag cccaagtcgc ttctttatta
      421 acagttaatg atttagtaat cattttttct ttatctggga aaacgaaaga tacttatgat
      481 tcattgaaaa tagctaaaaa aaatggcgca aaaattattg ctataactaa ctatattcat
      541 tcacctatag gaaagtctgc agacttagtt ttacaaacag ccattgaaga atttcttaat
      601 ggtggatctt tagctggaaa aatatcacaa ctttatattt gtgatctatt agttcatggt
      661 tatgaacaaa acaataatat tgattcagtt gaattaagag aaaaagtttt gcgctctatt
      721 attgataaaa gtattgagtg aactaccccc acttagctaa acctaacggt ttttaacgat
      781 tgaagtaggc aattcttggg atgagagcat acttgatagc ttatttttta ctaatagcgg
      841 tggctcttat tgaccaaata tccctgtagt tcctacggtt cttgatttta tctaaagata
      901 tttttatcgt ggaagctatt gatcaacttg aaagaaaaac gtgtagctca ttatcactag
      961 tttatcgagt atggatgaag caatggatca tcctctctta aataaattta ttaaggatct
     1021 gatccttcaa attttttcca tgatcgatga gcaggaacct actgaatcta aacgacatcg
     1081 ggcacaagga attattaaga ttgctaagcg aatgttttct ataaagggtg ttctaagcta
     1141 tacagtgtta attcaaaaga ttcccaatca tcttgtttat aagaacaacg ttgaagatta
     1201 aaaaagtgtt tcaatgctaa aattgcaaaa tacttcaatg ttatgagaca aactgtctat
     1261 aagaatcaat ttccacccat ttcatcactt gttaaaagtt tatgatcctt ttgattttta
     1321 tattaattta tctgtattta atacagattt tatttattag ttcatctgcc tattgtattt
     1381 tattttgttg ccttaccatc tgaagtaaat atactcttaa ttgtcaaatt aagttagaca
     1441 gatcatcaat acttacgtaa ctcaaaaata cttccaaagg tgttcgataa tccagtgatt
     1501 ttctaggaag attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc
     1561 ttggttgctt gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct
     1621 ggacataagt ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag
     1681 gacgggtgta tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga
     1741 tttttaaaag accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg
     1801 gttctacgta ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac
     1861 ggatgtattt ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg
     1921 accaaaagga tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta
     1981 ccgcactacg acctttcaga taccccataa aactggatat acatattttt ggtggtactc
     2041 gaacaagcat gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca
     2101 tttcgcacaa cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt
     2161 ttcgttgata ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta
     2221 aacttttatc gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc
     2281 actcactagt atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa
     2341 cgcgcggttt tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa
     2401 taaaagggtc taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct
     2461 aacttttgga ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc
     2521 gttcatgatt acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt
     2581 aaggctccat cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt
     2641 ctagtaagaa aacgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag
     2701 ttatatctaa caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac
     2761 cctaatttga tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca
     2821 aaaacgaaag gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa
     2881 tctacaacta aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc
     2941 cgttcccttc aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa
     3001 gcccaacaac gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta
     3061 aattagttga gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa
     3121 aacgattgga ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaagttcggg
     3181 agaagcacaa ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct
     3241 ctatcaataa aaagaaagtt cagcgcctga tcaagaagct aggaataaaa gtaacgacat
     3301 acacacgtaa atcacggcgc tacaattctt atcgaggaca agt
//
LOCUS       DACDHJ010000209         3332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDHJ010000209 DACDHJ010000000
VERSION     DACDHJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.213"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..367
                     /locus_tag="IV223_002343"
     CDS             155..367
                     /locus_tag="IV223_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712595.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(645..2444)
                     /locus_tag="IV223_002344"
     CDS             complement(645..2444)
                     /locus_tag="IV223_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8712596.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2568..3164)
                     /locus_tag="IV223_002345"
     CDS             complement(2568..3164)
                     /locus_tag="IV223_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8712597.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1215 a          600 c          586 g          931 t
ORIGIN      
        1 tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa aataaaaaat
       61 aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa aataaagaaa
      121 aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag taggaagatc
      181 ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg aacgtacata
      241 tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc aacttatcaa
      301 tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg ctgaattggt
      361 ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa taaagacaga
      421 agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat tattcttgca
      481 gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga atcaagagaa
      541 agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt ggcaggttat
      601 gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc gactcccaaa
      661 acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt cacactttca
      721 tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat catgcgactc
      781 atagcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg gtgggcagaa
      841 ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc aggcgtcaca
      901 gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg ttctgcttct
      961 tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt ctctaagata
     1021 actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt tttagaggtc
     1081 ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata agaatcaaaa
     1141 gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa ttctttcaat
     1201 gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc atcatgcatc
     1261 actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc aggatgatag
     1321 tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg ggtactcaca
     1381 cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata atccatattg
     1441 taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt gtttttcagc
     1501 gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc aggcaagaag
     1561 ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga catcacgata
     1621 ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat caatgcatag
     1681 tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata tcctaaagaa
     1741 gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc caaaagacgc
     1801 gatttggtca atgaccagct ttcccgaatg gctgctcgaa aaggacgatc ccgtagaatc
     1861 atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat ataaacgagt
     1921 aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat gaaagcgggg
     1981 atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg tagaatcaat
     2041 aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag ctgaagtatc
     2101 gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa aaaaacactc
     2161 atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa aactacgatt
     2221 aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc ataagacaaa
     2281 tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa gggtaaacaa
     2341 ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg tgttcctttt
     2401 aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc acctgctctt
     2461 cacattatag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg cagacccttt
     2521 cccagattta acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga
     2581 ccacttagat attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt
     2641 ttagcaatca aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc
     2701 aaacataacg catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca
     2761 aaacggtttt gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg
     2821 gaaagtatga atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg
     2881 cagattccaa tatagttata gataaatcca gcataagggc caaatatcag gacaccagct
     2941 gcagtactga tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca
     3001 aaaataatcg gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct
     3061 ttgaaaacac ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata
     3121 atggatatga aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt
     3181 agaggttctt tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata
     3241 tgacagaaaa caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa
     3301 acatccaact aaagacttat ttaataactt gt
//
LOCUS       DACDHJ010000210         3269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDHJ010000210 DACDHJ010000000
VERSION     DACDHJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.214"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..880
                     /locus_tag="IV223_002346"
     CDS             <1..880
                     /locus_tag="IV223_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8712598.1"
                     /translation="INEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGVSSAI
                     TTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIGHYTN
                     YAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTEDTLE
                     MLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYPDNLN
                     QYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCTLFGK
                     GFGNYDQDIKLKVYYS"
     gene            complement(953..1777)
                     /gene="nadE"
                     /locus_tag="IV223_002347"
     CDS             complement(953..1777)
                     /gene="nadE"
                     /locus_tag="IV223_002347"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8712599.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1789..3261)
                     /locus_tag="IV223_002348"
     CDS             complement(1789..3261)
                     /locus_tag="IV223_002348"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8712600.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT          911 a          692 c          600 g         1066 t
ORIGIN      
        1 aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa ggatcaagat
       61 catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt
      121 gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac
      181 agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg atatcagttt
      241 tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta ttggtcacta
      301 tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt ttatcgggaa
      361 agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca ttctctggtt
      421 taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg atacattgga
      481 gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg acaattggcc
      541 ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga gccctggaga
      601 ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc ttaatcaata
      661 cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg gagaaccctt
      721 gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt tgcgaatcga
      781 agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag gattcggtaa
      841 ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt ttctatttta
      901 taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat tgttatttcc
      961 agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg
     1021 cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa tcatcaattt
     1081 cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca tcttcaagat
     1141 ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga agcaattgtt
     1201 tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca tcaccaaatt
     1261 tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg gctcctgctc
     1321 tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata tttcctttgt
     1381 taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca tcaacagctg
     1441 gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca gcttttgcat
     1501 cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga tagttttcat
     1561 cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct gctaaagtag
     1621 aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat tttttcaaat
     1681 atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt gttggtttga
     1741 cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct atgcttctgt
     1801 atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat ggttccagca
     1861 ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat actcttccca
     1921 tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag gtaattcgta
     1981 aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa agtctcgaac
     2041 agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt cttctcgagg
     2101 gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat
     2161 gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg ataacttgat
     2221 cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata ctgcaccaag
     2281 ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat caattttagc
     2341 tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag catagatttt
     2401 tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata tataggccat
     2461 atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt ctttcgccac
     2521 gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta agaacacgca
     2581 gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat aagattggac
     2641 taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag cacgtacatt
     2701 gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg ctgcatccag
     2761 ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga cagatttgat
     2821 acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca gtgctgtttc
     2881 tactaattgg cagtgcgcta aaggcccttc cacttggatt aatggttcat ttgcaaaaac
     2941 tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt gtaaatactc
     3001 caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa
     3061 cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg
     3121 gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag ctcgacctaa
     3181 ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg tcaaactgtc
     3241 gtctgggtaa gtattattca tcttgtatt
//
LOCUS       DACDHJ010000211         3232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDHJ010000211 DACDHJ010000000
VERSION     DACDHJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.215"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(49..121)
                     /locus_tag="IV223_002349"
     tRNA            complement(49..121)
                     /locus_tag="IV223_002349"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Gln,seq:ctg)
     gene            complement(167..595)
                     /locus_tag="IV223_002350"
     CDS             complement(167..595)
                     /locus_tag="IV223_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712601.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(655..1413)
                     /locus_tag="IV223_002351"
     CDS             complement(655..1413)
                     /locus_tag="IV223_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8712602.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1708..1905
                     /locus_tag="IV223_002352"
     CDS             1708..1905
                     /locus_tag="IV223_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712603.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2011..2493
                     /locus_tag="IV223_002353"
     CDS             2011..2493
                     /locus_tag="IV223_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712604.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2551..3147)
                     /locus_tag="IV223_002354"
     CDS             complement(2551..3147)
                     /locus_tag="IV223_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8712605.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          961 a          540 c          633 g         1098 t
ORIGIN      
        1 aagattgttc ttttacaggg ggttcatttt ttcagcaagc acatcaaatt gggatggcag
       61 ggttcgaacc tacaattgct tgattcagag tcaagtgcct taccagtttg gctacatccc
      121 atttattcaa caaaataatc atagcacttt cctaaaaaaa cgcaagttat ttttcttgat
      181 cggatagttg ttgctttact cgtaaaaggc ggcgtactag tttcttttct aatccagcag
      241 gaagggcaat cagttctgct actgacaaga cggtgtccaa taactgatat tctttgacag
      301 gttcattcat aatatgatag atgtttgcta aacgttcaat atattcaaag cgagtatact
      361 ctgttcgagc aattagaaat ttcaataaat cggtggcttt ctcgtactct tgatctgcgt
      421 aataaacaag agattgacga tacaatttga tttccagttc attgagggta gcaggtctgc
      481 gacgctcgtt ccactcatta atagagactt taccttgaac atttcccttg cagtcacgga
      541 caaaaataat cggaatttca tttggatcca tttttttatg tgtgaatttt tccattatct
      601 acgctccctt caaaggtttg gtatcttgag tataagggat ttttgtttga attactagct
      661 tttctccaaa gttagcatct gtccacccat tgcagtgaaa atcgccgaaa aatcgttgat
      721 cggtactgct cgatttttta caccataggg atcgttgaca taaatatttt ctttgtcata
      781 tccagtgact acacaagcat gaacaagagg agtgactctt acttctccat tatttgtggt
      841 ccaagtcata aaatcagttt catctgggac ttcaaaattg atagtcacag aagtccagac
      901 aggatttcct ttagatagtg cttttttgat atcatcgagt tgagcatcgg tttttggaaa
      961 agtgtgataa tccgtttgga cgatttgctg agcaagggag gcaactgggg gaacatagac
     1021 gaccatagcc cagtctccgc cactgatatc accaacaaat ccttcattag gatcgccatg
     1081 agtcccatct gcattgaaaa cagggacgta atccaattgc tcagctaact ggtttttagt
     1141 tgtttcataa ccataaaaag ataaaagcat agaaagcgaa gtgacttcac agccattttc
     1201 taaagaagga gaatcaaact ggctctctaa tggaacatct agtaaaactt ctgctttctc
     1261 ttttgtcttt ttcgtttttt gtgttgaact tttttgtgca gtgttcattg tatgttctgg
     1321 agaatggata tgtaggtaat aacttgtacc gcttaataaa agagcagctg tgccccctag
     1381 aaacaatagt tttttgaacg tttttttctt catttgaaat ctcccttttc agctgtttat
     1441 ctttcctatt ataaatgtat ttttattgga aatggaagta ttatatttta atatgtagga
     1501 aataattagt ttttaaggaa tatgttttaa ttttttgaaa aaaattcttc taaatacaac
     1561 agctgtttta attgtgaaaa cgaaaacagt acgctataat gcctgtaagg ttgattttta
     1621 actgtaaata aaaatcaatg agttaaatct ccaaagaaat agcagtggta atgtggagaa
     1681 aagttccagc agtttaggag gattttgatg acggaagaaa tgattaattt aggagaacaa
     1741 tatgcatgca aaccaattgg ttttacaaaa acggtcattg gtgaagttgt atcaaaaatg
     1801 acaaactgcg ccgtggtcaa agttgcccaa tgtgctgcag aggaccaaga attattagat
     1861 gaaaaagcaa gcatggttgt tgcaaaatat gatacatttg aataaagaaa acaattggac
     1921 gtgcggcgtg ctgcaagtcc aatttctttg agaaggtaaa tcagtattct tttcctattt
     1981 tctggagaag aaaaagcgag gcgagttgtc atggtaacat tctttacggt tctattgttt
     2041 ttattcgttg ttgtgatgtt tttatttctt tggctggttc gaaaagaaat tatttatcgg
     2101 actgtgcgaa atcgatgggt ctacttggtg atacccttct tagtgtcact agtcatttgg
     2161 tatacactca tcagtcatcc tacagcagat gaattggcaa aagggatctt gtcagcaatg
     2221 atttttatta gttttttgtt ggacagtaga ggaatcacag aagaaggact tgttttgaat
     2281 agctttgaca aaaaaggtgt tcctttttct gagatcaata aaattgtatt atatcagcca
     2341 aaaggttcta aaatcgtaaa aatgaatttt ttccgaaacg gatggcgagg accgatgctg
     2401 aaattttctg catcattaga agaattagtt ccatttttgt cccaacgctt aaacgatgag
     2461 gcagagattg atattatgat tgatccagaa taaaaacaga ggttgcgaca agatgtttgt
     2521 cacaacctct gttttttatt tcaaataaaa ttatccaaat acgcggaagc gatcggcatc
     2581 gttcatgtat tctttttccc cagccggtgt ttctggtgaa ccgaatacta attgcgaacg
     2641 tagtttccag tttgaaggga tattccattc ttttgcaact gcttcgtcaa taactgggtt
     2701 gtaatgttgc aagtttgcgc ccaatccttt atcaactaat gctacccaag tattagctgt
     2761 tgcgattccg tttgattgtt ctgaccaatc tgggaagttg tctgcatata attcaaattg
     2821 ttcttgtaga ccttttacca cgtcagtatc tttgaagaat aatactgtac cataaccatt
     2881 tttgaaacca gctaatttgt tttgtgtatt tgggaaagct tctgctggag tcaatgggcg
     2941 aagtgcttct tcagtgattt cccaaagttt ttcatgtgca tctccgaaaa ggataactgc
     3001 gcgtgttgat tgagcattga aagcagttgg gctttcttta attgcttctt tgatcaaagt
     3061 tgttaattct tcattagaaa gtgaaacatt acgtcctaga tgatagattg aacggcggtt
     3121 tttcaaagtg tcagtaaaag ttgtcataat aaaatttctc ctttgatttg cgttttgcat
     3181 ttttttaatt gattacgaaa tgaagtatag caccttacaa aaagtaagta aa
//
LOCUS       DACDHJ010000212         3183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDHJ010000212 DACDHJ010000000
VERSION     DACDHJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.216"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..1852
                     /gene="alsS"
                     /locus_tag="IV223_002355"
     CDS             188..1852
                     /gene="alsS"
                     /locus_tag="IV223_002355"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8712606.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1865..2575
                     /gene="budA"
                     /locus_tag="IV223_002356"
     CDS             1865..2575
                     /gene="budA"
                     /locus_tag="IV223_002356"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8712607.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2705..>3183
                     /locus_tag="IV223_002357"
     CDS             2705..>3183
                     /locus_tag="IV223_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8712608.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINA"
BASE COUNT         1067 a          521 c          708 g          887 t
ORIGIN      
        1 tttcaattaa gaatgacagt ttttatgtaa aaactgtcat tctttttcat tttttgaaac
       61 atatggctaa acaaatgaaa taaaacgtgc tatacttttg aatgaaacag gtttagaaac
      121 tgtatcaatt atttgtgatt atttctaact aataattaaa aataaagcat aaaaaggacg
      181 atgttcaatg gacgaaaaaa tgaaacaagg ttcgaaatct gtagtagaaa gtttgattaa
      241 tcatcatgtt gattatgtat tcggtatacc aggtgcaaaa atcgatggtg tatttaatga
      301 attagaggat caaggtcctg aattaatcgt gactcgtcac gaacaaaatg ctgcatttat
      361 ggcacaagca atcggacgta ttacaggaga gccaggagta gttatcgcaa caagtggtcc
      421 tggtgctagt aacttagcta caggtttggt aacagcgaca gcagaaggtg acccagtttt
      481 agcaattgcc ggacaagtaa aacgaagcga tctattgaag ttgacccatc aaagcatgga
      541 taatgcagca ctgttccagc caatcacaaa atacagtgca gaaatccaag atccagaaac
      601 aatttcagaa gtgattgcca atgcttatcg tatggctaag agttcaaaaa aaggcgcgag
      661 ctttatcagt atcccgcaag atgtagtgga tgcacctgtc caagggaatg tcatcaagcc
      721 attgagcgat cctaaattag gttctgcttc tgcagatgat atccgttacc tagctgaacg
      781 cattagagaa gcaaaactac cggtactatt agtaggtatg cgaggatcaa gtgaaaaaga
      841 aacgttagcc atcaggcaat tagttggaaa gacagcattg cctgttgtag agacattcca
      901 agcagctgga gtgatctcac gtgaattaga agcgcatttc tttggtcgtg tgggtttatt
      961 tagaaaccaa ccaggagata tgcttttgaa acgaagtgat ctagttattg ctattggcta
     1021 tgatccaatc gaatatgaag caagaaactg gaatgcggaa aaagatgcgc ggatcattgt
     1081 gatcgatgaa gcgccagctg aaatcgatcc ttttatgcag cctgaaagag aattgatcgg
     1141 tgatatctca gccactcttg atttattgac aggttcatta gagcctcaac aagtgtcaga
     1201 agatgcaaaa gaatatttag catctcttca agcagaatta acggagcggg acattgttca
     1261 atcaaaagga gaagcaggta tcttgcatcc tttagaagtt attaacacgc ttcagtcaaa
     1321 agtaacagat gatatgactg ttaccgtaga tgtaggtagt cattatattt ggatggcacg
     1381 ccactttaga agttatgaac ctcgtcattt gttattcagt aatggtatgc aaacgcttgg
     1441 tgttgcattg ccatgggcga tttctgctgc attggtgcgt ccaaatacac aaatcgtatc
     1501 agtttccggc gatgggggat ttttattctc tgcccaggat ttagaaacgg cggtacgtaa
     1561 aaaattgaat atcgttcatt tgatttggaa tgatggacac tacaatatgg tagagttcca
     1621 agaaaaaatg aaatatcagc gggcgtcagg tgtagacttt ggtccagttg attttgtgaa
     1681 atatgcagaa gcttttggcg ctaaaggaat ccgagctaca agtgtagagg aattagaaaa
     1741 agctttagaa gaaggatttg cgacagaagg acccgtaatt attgatattc cgattgatta
     1801 ccgtgataat gagaaattgg gagaaacaat cttaccagat caattctatt aaggaagtgt
     1861 cgacatgact gaaaaaatct tataccagca cgggacatta ggagctttga tggctggctt
     1921 gatggatgga acagaaacga ttgctcatat attagaaaaa ggaactctgg gattgggaac
     1981 attgcatggg cttgatggtg aagtcatctt tttagatggc gtagcttatc aaggaagatc
     2041 agatggctca gttgcccaat tagatggaag tgaacttacc ccctatgcag caattacaga
     2101 ttttacgcca gatactagtt tttctgtctc aaaaactgca gatggagaac aattgaaaaa
     2161 agacatatta gtaagagaag ccggggaaaa cctatttttg gcagtgaaga taactggatt
     2221 attcaaaaat atgcacatcc gtatcatgcc gaagcaagaa aaaccttaca gaagactagc
     2281 aaaaatttca gagtctcaac cagaattcca acaaagcaat gtccaaggaa cattagtcgg
     2341 gttctttacg cctgaattat tccaaggagt tgctgcagcg ggatttcact tgcattttat
     2401 cgatgataca catacatttg gcggacatgt aatggatttt gaagtggctg aaggaaaagt
     2461 tgagattagc cgcatctcgt cccttgtaca gcattttcca gttgatgatc cgacttttgt
     2521 tcacgaaacg attgattatg ctgatttagc agcagaaatc caacaagcag aatagaaaaa
     2581 gacagcgaga tgcgttcaag gtgattaacc tgacgtatct cgtttttttg aggaggaaac
     2641 ctagcgttta caaaaaaact gagctataat cttctccata gatcaaaaag gagcggtttt
     2701 tatcatgtgg acagaaaaac agaaaaaaga tgcatacgaa attttattat tacagcaaca
     2761 aggattatta gaagcagaag ataactggtt agcaaattta gcaaattcat ctgcattgtt
     2821 gaatgagact ttgcctgaga ctgtctttgc aggatattat ttattcgatg gtaatgaatt
     2881 gattttagga ccgttccaag ggcgagtttc ttgcacacgt atcaagatgg gaaaaggagt
     2941 ttgtggagaa tcagctgaaa aacaagaaac attgatcgtt gatgatgtca aaacccatgc
     3001 aaattacatt tcctgcgatt cagccgcaca atcagaaatc gtagtgccaa tggtaaaaga
     3061 aggtaattta atcggtgttt tagatatcga ttgcggagta acgaaagggt atgatgaaat
     3121 cgatcaagca tttctagaaa aatatgtaga tcttttatta gcaaatttta atataaatgc
     3181 ttt
//
LOCUS       DACDHJ010000213         3164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDHJ010000213 DACDHJ010000000
VERSION     DACDHJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.217"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            140..1792
                     /locus_tag="IV223_002358"
     CDS             140..1792
                     /locus_tag="IV223_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8712609.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            2136..2501
                     /locus_tag="IV223_002359"
     CDS             2136..2501
                     /locus_tag="IV223_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP8712610.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            2589..3032
                     /locus_tag="IV223_002360"
     CDS             2589..3032
                     /locus_tag="IV223_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712611.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
BASE COUNT          887 a          557 c          749 g          971 t
ORIGIN      
        1 tagagacatt ccaattgtca gatttatttt ataatacttt gggtggcggc attagtgggg
       61 ttatatatta tttgagctga tatattcatc ctaaccttaa aaataaatga gcagaatttt
      121 aaaagattgg agagattcta tggatcataa tgcaaattat tatcattggc ttacaaatgc
      181 caatgcagaa attgtagagg aactgcggtc atattccgag aaggacattg aggactcctt
      241 ctataaaaat ctgtcctttg gtaccggtgg tctgcgtggc accattggtg caggcactaa
      301 tcgtatgaac gtacacaccg taggtaaggc aagccaggga ctgtcagact atctgaataa
      361 gacctatgaa accccttctg tcgtcgtggg ctatgattcc cgaatcaagt ccgatgtatt
      421 tgcaaaggtt gccgcggaca tctttgctgc aaacggtatt caggtgcatc tgtggccgga
      481 gctgctgcct gtgcctaccg tatcctttgc tatccgttat ctgaaggctt ctgccggtgt
      541 tatgatcacc gcatcccata atccttccaa gtataacgga tataaggtct atggcgcagg
      601 cggctgccag atcaccaccg aggctgccgc tgagattctg gctgaaattg aaaagctgga
      661 tatttttgca gatgtgaaaa tgggcggcga cgcttccaga atcgcttcta tccagccaga
      721 ggttctgact gctttcattg aagaagtaaa gggccagtcg gttctgtttg gcgaagaagt
      781 ggacaagaat gttgctatcg tgtattctcc tttgaatggt accggtctgg tgcctgttac
      841 ccgtactctg gcagaaatgg gctacagcaa tgtgaccgtt gtcgaagagc agcgtctgcc
      901 agacggtaat ttcccgacct gtccttatcc gaatccggaa attaatgaag ctatgtctct
      961 gggcatggag tatgccaaga agtataatgc tgatctgctg ctggctaccg accctgactg
     1021 cgaccgtgtg ggtatcgccg tgaagaatgc agaaggggat tatgtcctgc tgtccggtaa
     1081 cgaaactggt ctgttgcttc tggactacat atgctctcag agaattaagc acggcaagat
     1141 gcccactgac cctgtgatgg tcaagaccat cgtgactatg gatctgggcg agcggatcgc
     1201 ttccaactat ggcgtgcgta ctgtcgatgt gttgaccggc ttcaagttca tcggcgaaca
     1261 gattggtatg ctggaaaagg caggcaaggt ggacagctat attttcggct ttgaggaatc
     1321 ctacggctat ctatccggca gctatgtccg tgacaaggat ggtgtcaacg ccgcttatct
     1381 gatctgcgag atgttctctt actacaagac tcagggtatc agtctgtttg ataagctgaa
     1441 cgagatttat gctaagtatg gctactgttt gaacaccctg cattcctttg agtttgaggg
     1501 tgcggcaggt tttaccaaga tgcagaaaat tatggttgaa ttccataaag gtcttgatat
     1561 gattggaggt aagaaggtca ttgaaacctt ggattacagc acgggactga atggcctgcc
     1621 gaagtctgat gttctcaaat atctgctgga gggcaactgc tctgtagtgg ttcggccttc
     1681 cggcactgag ccgaagctga agacctatat cagcgttagc gcagaaagca aggaagctgc
     1741 cgaagtaatg gaagctgtta tggctgctga actggctgaa atgtttaagt aatactatga
     1801 aaaagatctg agtgtcaatg tccgaagtag caacatctat gtttaataaa aatttcctgc
     1861 tttacggttc tgtttacata attgaggtaa aggggccgac tgtgtggatc gaaaaggcaa
     1921 agaagtgtta acagagacac agcagcctga tgaagcaccg atcaaagcag taagggagaa
     1981 ataagagata ttagagtttt aataatgtat tcagcaatag agatgaaagg agtagaatat
     2041 gaataaaact attgaaataa taaaatgagg gggaaagaaa taaacgtgat attattagtt
     2101 ttgagaaggc gttttcaata ttcgctattg tattgatgta ctattttcag caatcgtatt
     2161 tgccagcaat actgaacaaa gcaatatcta ttggtgatac aggagtgcat atattcttct
     2221 tttgctcagg ttttgggcta tatctgtcat atttaaaaaa gcctattaca ttgggtgaat
     2281 ttttacagaa acgatttatc aagatttata ttccgtatat tatcgtaatt acggtatcag
     2341 tgttactacc ttatatgaca caagatttaa atgctgcaaa agtatatttg tctcatgttt
     2401 tgttatttaa aatttttcta ccagtatatg aggagtcact gggtcccttt ggtttatttc
     2461 gacaattatt cagttctatt tcttattcat accaattgta aaactataga gaaaaatagg
     2521 aatgaaatga ttgcttgttg caagctgagt tattagtatc tgggctatta tgatgtttca
     2581 tacaggatat ggaagcatac gagttcttgg gagctgcttt gcgcaatata tttgggaatt
     2641 tactttcggc atgtttggtt gcagagtatt tagtgaaaag aaaaatagta agatttaata
     2701 atatattcgt gattatggcg tccattgttg gaattggttt tgctggtata tcgaaaatta
     2761 ttggtggact gatggagtct attaatgata tttttgcatt cttcggttac ggtgcaattg
     2821 caattttaat gtatcaagtt attccattta gaaagataat gcctaggatt tcaagatact
     2881 catatgatat atatctactc catattcttg tatttagtac gattcatcaa attatcccta
     2941 acttggtttt ggcggcattt gtttccattc ctataacgtt ggtgttgtca tattattata
     3001 gtattatgtt atatagactt aaattaaaat gatagcataa ttgtcgcctc taccgggcgg
     3061 ttgccatcct gattaacagc ctccacgcgg cggatggtgg gcacttaggt agttcgtaac
     3121 ctagtggtag atggttggta aaaaggagat aaaactaaat gatt
//
LOCUS       DACDHJ010000214         3093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDHJ010000214 DACDHJ010000000
VERSION     DACDHJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.218"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..1086
                     /locus_tag="IV223_002361"
     CDS             4..1086
                     /locus_tag="IV223_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8712612.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1180..3015)
                     /gene="lepA"
                     /locus_tag="IV223_002362"
     CDS             complement(1180..3015)
                     /gene="lepA"
                     /locus_tag="IV223_002362"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8712613.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT          930 a          641 c          597 g          925 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc agaacggcca tgaaggcttc ttgcggaact tcgacggagc cgatttgttt
     1261 catccgcttc ttcccttctt tttgtttctc taagagttta cgtttacgag aaacatctcc
     1321 accataacat ttagccaaga cgtttttacg caaggctttg atgtctgaac gggcaacgat
     1381 tttttgtcca atagctgctt ggattggcac ctcaaattgt tggcgtggga ttagtttttt
     1441 cagtttttca acgattgctt ttccacgttc ataggcaaag tctcggtgaa cgataaagct
     1501 taatgcatcg actttttctc cattcagcaa gatgtccatc ttcactaact tgcttttttg
     1561 ataaccagac atttcataat ccaatgaggc atagcctttc gtgcttgatt tcaatttgtc
     1621 gaaaaagtca aagacaattt cagaaagcgg aatattatag acgacattta ctcggtaatc
     1681 atctaaataa tccatcgtaa tgaattctcc ccgttttcgt tgagaaagtt ccattactgc
     1741 accgacaaaa tcatttggga ccatgatctg tgctttgacg aatggttctt ctacatcctg
     1801 aatcgttaca ggttcaggaa aatctgccgg gttatctact gtagcagtcg taccatccgt
     1861 tttattaaca tggtaaataa cggatggtgc agtcgtgatc aattctagat tgaattcccg
     1921 ttccaagcgt tcttggacga catccatatg aagaagtccg aggaatccac agcggaaccc
     1981 aaaacctaat gcttgagagg tttctggttc gaattgtaaa gcagcatcat tcaattgcag
     2041 tttttctaaa gcttcccgca aatcgttgta acgagaagta tcaattggat aaagaccaca
     2101 ataaaccatc ggattcattt ttcggtaacc tggtaaagct tcagcggcag gattgtctgc
     2161 taacgttact gtatccccta ctcgtgtatc ttggacagtt ttgatacttg ctgtgatata
     2221 accgacatca ccgaccatca aaaagtctct agcaactgct tttggcgaga agaccccgac
     2281 ctctgtaaca tcaaatgttt tgccattact catcagtttg attttgtctc cgggtttgac
     2341 cataccgtcc gtgatccgga catttagaac aacaccgcgg taactatcat aaatagagtc
     2401 aaagatcaaa gcttttagcg gtgcatctaa gtctccactt ggtgcaggaa catattccac
     2461 gatctgctcg agaatatctt caatcccaat ccctgatttg gcactggcta atacagcttc
     2521 actagcatcg attccgatta cgtcttcgat ttcttgacgg acacgttcgg gatctgctgc
     2581 gggtaggtcg attttattga tgactggcag gatctccaaa tcattgtcta acgctaaata
     2641 aacattggcc aatgtttgtg cttcgattcc ttgtgctgca tcgaccacta aaacggcacc
     2701 ctcacaggca gcaaggcttc gagagacttc ataggtgaag tcgacgtgcc ctggggtgtc
     2761 aatcaagtga aagatatatg tttctccatt tttcgctgtg taattgagtt ctacagcatt
     2821 taatttaatc gtgattccac gttcacgttc caaatccatc gagtctaaca gctgatcttg
     2881 catttcgcga gacgtcacag tgtgcgtcat ttctaaaata cgatcggcca atgtagattt
     2941 cccatggtcg atatgagcga tgatcgaaaa attacgaatc tgctcttggc gttctttcat
     3001 tttctttata tccatagcgt ctcctctttt catataatca gcactttcca ttataccaac
     3061 gaaaagcagt aatttaaaga gtttttaatt agt
//
LOCUS       DACDHJ010000215         3081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDHJ010000215 DACDHJ010000000
VERSION     DACDHJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.219"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..747
                     /locus_tag="IV223_002363"
     CDS             139..747
                     /locus_tag="IV223_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8712614.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            747..1088
                     /locus_tag="IV223_002364"
     CDS             747..1088
                     /locus_tag="IV223_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712615.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            1330..>3081
                     /locus_tag="IV223_002365"
     CDS             1330..>3081
                     /locus_tag="IV223_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP8712616.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEET"
BASE COUNT         1058 a          413 c          670 g          940 t
ORIGIN      
        1 gggacttttt ttaatacgat tacaatcgat gagcaaatat tatttccaaa agcctattta
       61 cctgatgaag cagatggtgt tcaacatatt cgttttacat tagaatttcc acaatatatt
      121 gaatggagtg aaagataaat gggagaaaaa agaagtaaaa ccggaattat ttctgtcgaa
      181 aaacctactt ggtttccttt ggaagatgag acagggaatt ttccggtata tggtactgcg
      241 ttgccaatgg gaacagcagt aagtatcaaa ccaacagcta attatgaaac aacacaagat
      301 tatggggatt cagtggttca agatcaattt acagcttttg gtggtgcaga agttgaactg
      361 gaagcaaatg gatatactca taaagtttta tctacaatta ctggagggaa aattgttaaa
      421 ggtggtgcct tgcgttctgg agaagatatt gcgcaagatg gtgcgtttgc atatagacgt
      481 aaaaaatcga atgggaaata ccgttatacg gtcttttata aaggacagtt tgctctagat
      541 tcagatgaaa catcaacgat tgaaggaagt aaagtaagtt ttactcatcc aacttggaag
      601 ggttcatttg tagatgtacc aggacttgga tatatgtatt cagttgatga agatgatgaa
      661 ggcgtggata aagcaatgat cggaaattgg tttacaaaag tagcaattcc aattgaagaa
      721 gcagaacttt caggaggtac agaataatgt caaaatatca aacaacgatt aaattaacga
      781 aaaaagacga agaaggtaaa tatgagcaag tacaatttaa atctgctgaa tttttaccag
      841 gaacagttgt agaagatgct gcaggagtta tggaagaaat gcaaacagcg actgataagc
      901 aatcagttaa aaaagcttta agtcgtgctt attcatttat tgcagatact ttatttgagg
      961 gacaatttac tggtgaagat tattgcaaag gaattgatgc tcgtgagatt gcctcattga
     1021 caggaaaatt attgaagtct gttactgcag gttttgatga aacttataca gagacgaaaa
     1081 aaaagtaagt gaggcgctca agtcaccttc atttaagtat tcgattactt accgagaatt
     1141 agatataaag acgcaattac tcgaagcagg ttggacgtta ccagaaattg aacgtactga
     1201 cttggatgag ttaatgcgtc tttttgcttt tagagatgcg gtaaaagagc atgaagatgt
     1261 tgagtactat gataatttca ctcaatttta ggaggtgata ctttgaataa cgatgaccta
     1321 attctgaaga tgatactaga tgaatctggt tttactgccg gtatgaataa tgctgtcaaa
     1381 aagttgaact cttttgatga aacaatcgaa agaaaaagta gaaatagtgg cagttcatta
     1441 ggtagtattt ggaaaatatt tgctggaagt tttctagcta gcggagtaac tagaatcgta
     1501 ggagctggct ttgatctaat caagggttcc ataagtggag cggttgatcg agtagatacg
     1561 atgaataacg ctctacgaaa tttccaaaat atgggattta gtaattcaga aattatgaag
     1621 aatatcggaa agaatgggct tttatctcaa ggtattcaag gacttcctac tgctttgaat
     1681 gatgcgataa gtcatgttca acttcttgct tcttctacag gtgatttaac tcgttcaact
     1741 caaatattta aagctttaaa tgatggaatt cttggtttcg gtggttcaac tgatcaagtt
     1801 aatgaagctg ttattcaatt atctcaaagt ttctcaaacg gaaaagtaga cgcacaaact
     1861 tggaactcaa tgatcaacgc tcaacttgga cctactcttt ctgctattgc taaaaagatg
     1921 ggaattacaa tgggagatct gaaagaaggt ttatctcaag gtaagatttc tgttgaagaa
     1981 ttccaaaatc aattaataga aatggatacc aaaggtggcg gaggacttaa atcattaagt
     2041 caaatcgcta aagattctac taagggaatt aaaacctcta tacaaaatgc aaaaacagct
     2101 gttacacgtg gtgttggtga ggtaatagaa ggattaaata aagcattagt agattcagat
     2161 ttaggtggat ttaagggaat tattgataaa gtgggcagtt caatggaatc gttcttaaaa
     2221 gtaattgctg caaatattcc tacagcagta tcatttttaa gtaatttatt taacgaaatt
     2281 caaaaatttg gttctgcttt gaaattcatg atgccctttc ttgttcctgc agctaccgct
     2341 tttggagcgt tcatgtttca acttaaaggt ataccagcaa taataaaaag cttcaataat
     2401 tttaagaatg ccataattgg cgttggaaat tcattaaaga ttatgggggc aatagctgct
     2461 gctaatccat ttgttttaat tattggtgca gtcgttggtg caatagctgt tttcggttac
     2521 tttatggcaa ccaatgaaga gcttagaaac aaggttatat ctatttggaa tgatgtgaaa
     2581 gattccgtaa ttggtgtatt aaaaaatata aaggactggg gaattgatac ttggaattct
     2641 gctaaagaaa tggcatcaaa tgcagttgag ggtgtcaaag atgcttggtc aggaacaaag
     2701 gaatggtttt ctaacacctg gaaaggtatt aaggatgggg ccacaggttt atttgataaa
     2761 acagtagaga cttcaagaaa tgcagtcgat agtgtaaaaa atgcatggtc taatatgaag
     2821 aaatggtttt ctgatacttg gcaaagtata aaagattcag ccagagaaaa atggaatgga
     2881 atcaaaggtt ctattatgtc agttgctggt ccattgatta caggtataaa gaatgcattt
     2941 ttacatgtta ctttttatct agagacctta tggaataatt tagttgagat tggtaaaaac
     3001 gtctttgaga ttttaaaaaa tgttatactt gcgccggttt tgtttattac ctccatgatt
     3061 tctggtggat gggaagaaac a
//
LOCUS       DACDHJ010000216         3079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDHJ010000216 DACDHJ010000000
VERSION     DACDHJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.220"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IV223_002366"
     CDS             complement(<1..2232)
                     /locus_tag="IV223_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712617.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT          843 a          698 c          509 g         1029 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttc
//
LOCUS       DACDHJ010000217         3050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDHJ010000217 DACDHJ010000000
VERSION     DACDHJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.221"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            728..2644
                     /gene="ltrA"
                     /locus_tag="IV223_002367"
     CDS             728..2644
                     /gene="ltrA"
                     /locus_tag="IV223_002367"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8712618.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            2761..3050
                     /locus_tag="IV223_002368"
                     /pseudo
     CDS             2761..3050
                     /locus_tag="IV223_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
BASE COUNT         1166 a          492 c          636 g          756 t
ORIGIN      
        1 gaatttggga atagtgtgac ctgtttagcc taaggataaa agaggctagt tccaacgaac
       61 attggaataa ctttactatc gagaaatcaa atgacggggt aacgccctga agggtttcca
      121 ctgatactgc gacatgcgtt agtaggatgt gcatccaaac gcgtatgaag ctcgctgaag
      181 tcggcagaag ggtaccgtaa ggaaagacac gatacgtatc taccacgcaa gtgctctaga
      241 ggtagggtat gtacctgata tgtcgggtgt cctaaaaagt tcaataaggt gagaatgatt
      301 tgattatctg acaaacttgc gaatggaatg tctgaaacga ataactcata gaaatgtgag
      361 ggggaagggg gatacgtcct ctttccattg actcgaggtt gagtaaaaat gtgtttctat
      421 gaaaaacctt gcatggttaa aggctatgga gtagtcaaca ggcaaaagca agcacctaag
      481 ttgaaatcta tggatagata gtaaggaaca ggggaagcat ctagcacgga gggagcatac
      541 cgaagatgtg tttctaagga aaaaccgaaa tcggttataa cttaggtgaa tagatgtgaa
      601 agtagagcca cgaccagtga aaatttcgta atgaaattgg aggaacaggc tcaagtcgtt
      661 atttataata taagaaacct atttaaaatc atggattctc gtaagactaa aaaggtcacg
      721 aaccagaatg gagcgtgata aaaccatgac taacacaaaa ggaattagct taaagaacaa
      781 acaactcaga cacgccgaat actacgacat gactgagact ttcgatgaat tgcacgcaat
      841 gagtgttgca aaatcaaatt ttaaaaatct catgaaattc attattgatg acaataatat
      901 ctttctagcg tatagaaata tcaaaaagaa taaaggaagt gcaacggccg gatacgatgg
      961 tgtcactata aaagacattg aaaaattggc atgtggtgac tttttaacta ttattaaaag
     1021 gcgatttaag gattacagac caagaaaagt taaaagagta gagatcccga aaccaaacgg
     1081 aaaacttcga cctttaggaa ttccttcaat atgggacagg atagtccagc aatgtattct
     1141 acaaatttta gaaccaatat gcgaagcaaa atttaataag catagctatg gattcagacc
     1201 aaattgttct acagaaaacg ccatagcaga ttgtcttgct agaatcaatc aaagtaagat
     1261 gaattatgtt gtagatattg atataaaagg attctttgac gaagtaaacc ataccaaact
     1321 tatgcgtcaa atatggacaa tggggattcg agataaacaa ctccttgtaa ttattcgtaa
     1381 gatgttaaag gctccgattg ttatgcgaaa tggcaatgtt gtttttccaa ccaagggtac
     1441 tccacaagga gggattttaa gtccactgct tgcgaatata tgcctgaatg aatttgattg
     1501 gtggatagct aatcaatggg aggaacgaac tgtcaaagag ctgaaacacc actatcgaaa
     1561 aaacggagca tgggataaag gaaatatcta ttcgaaatta agaagccaca caagtttaaa
     1621 agagatgtat ctggtacgct atgcagatga ttttaaaatt ttctgccgaa caggagaaga
     1681 agcccaaagg gctttttatg ccacacaaaa atggctcaga gaaagattaa agctcccaat
     1741 ttcaatggag aaatctaaag ttacgaacct aaataaagct gaaagtgaat tcttgggttt
     1801 ttcaataaga ctccacaaaa agggaaaaaa gaaagtggct ttcacgtacg tttgtcgcaa
     1861 agcagaagaa aacattaaaa ataaactaaa acaacagatt aaaaaaattc aatacgctgg
     1921 agcaaaaaaa aataggcacc gagcaatagg tgactacaac atgatggtca tcggtatcca
     1981 caattactat cggatagctt cacattgtag tgtcaatttc actgagattt atagaagtgt
     2041 taggacagta atagaagcaa gactgaaagc tagaggaatg acgaaaaatg gaatctatgg
     2101 acaagatcat gaaggatacc gtccgtatct aaagtctaag caaatgagat atattgaagg
     2161 ttttccgata cttccaattg gctatattaa aaaaagaact ccactcaaca agcggagaaa
     2221 tctaaataaa tataccaagg aagggagaaa gctcattcat caagagcaac aagctataga
     2281 aagctggaaa atcaagtatc ttgtaaaagg ttttagtaac aataagaaat acacggtgga
     2341 attttctgat aacaaaatat ctaagtttat ttcccaaaaa gggaaatgcg ctatcacagg
     2401 aaacgaactt ttgatggaag aaattgaatg ccatcacatt aacccatatc atttgtccaa
     2461 agacgatagt tacaagaacc ttgtcattat ctctagagaa atccatagat taatccatgc
     2521 aactagtgaa gaaactattc tgaaactgct tcggttactg aaactaaatc actatcaaat
     2581 tagaaaacta aacacgtatc gaaaaaaagc agctaatttt ccaatatcaa tcaaaatgaa
     2641 ttagatagga aacaaattat attgtaatta acgatggaac gccgtgtgcg gtgaaagtcg
     2701 cacgcacggt gtggaccggg ggaaaagacg gagataatat caaactctta cctatcggaa
     2761 ttgccaccaa tggataatat ggaaggaatc atgacagtca cagctggtag aaacatgttg
     2821 tgggatttgt ttatccaatc ctatcagcaa ctttatgcga agtatgggga acaaaacgca
     2881 gcgattatca aagaaaattg tcagacacat atttatatta tgtcgacaga cgataatacc
     2941 atagaagaat tttccaaaaa agtggggaat aagacggttg aacaagagaa cagtaccatc
     3001 aattcattag gtatgaatac tcatatcaat cgcaatgttg attcagatcg
//
LOCUS       DACDHJ010000218         3018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDHJ010000218 DACDHJ010000000
VERSION     DACDHJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.222"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..1555
                     /locus_tag="IV223_002369"
     CDS             38..1555
                     /locus_tag="IV223_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8712619.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1545..2816
                     /locus_tag="IV223_002370"
     CDS             1545..2816
                     /locus_tag="IV223_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8712620.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
BASE COUNT         1037 a          447 c          673 g          861 t
ORIGIN      
        1 ggtgtattat tcatgtataa attaaaggag gtcagttatg gaagccacaa tgttgattag
       61 tagtgtcaag cgtgtaggca aagttgatcc gcggttgtac agttcattta ttgaacatat
      121 gggacgagca gtatatgaag ggatttatca gccagatcat ccatcagcta ataaaagtgg
      181 atttcgccaa gacgtcattc acttggtaaa agaattgaac attcccatga ttagatatcc
      241 tggtggaaat tttgtgtcag gatttcgttg ggaagacagt attgggccca tagcaaatag
      301 acctagaagg ttggatttag cttggcggac aattgaaacg aatgaagttg ggattcatga
      361 attttataac tggtgtcagc atgtgggggc cgaaatcaat atggctgtca atctgggtac
      421 tcgtggagta gatgcagctc gtaatttagt cgaatattgt aattttccta aaggaactta
      481 ttggagtgat ttaaggcgtt cgaatggtca agagagccct tttggaatta agacatggtg
      541 tttagggaat gaaatggacg gtccttggca gattggacat aaaacagcgg aagaatatgg
      601 acggttagca gaggaaacgg ctaaggcgat gaaactcgta gatgattcaa ttgaggtggt
      661 tttatgcgga agttcgaatc ggcaaatgcc tacatttggt gattgggagt taacagttct
      721 tgatcaagcg tacgatcaaa tagattattt gtctctgcat caatattatg gcaaccaaaa
      781 aaatgatctt tctcattatc ttgcacgttc cttagacatg gatcggttca tttctgaagt
      841 gacagcgatg tgcgatacag tcaaggcgaa gaaacatagt aagaagcaga tcaacctgtc
      901 ctttgatgaa tggaatattt ggtatcattc agcggaacaa gatgagcaag cagcgccttg
      961 gcagattgct cctcctttac tagaagataa ctataatttt gaagatgcat tactattagg
     1021 ctgtctatta atcactttat tgaaacatgc agatagagta aagattgctt gtcttgctca
     1081 attggtaaat gttattgcac caatcaggac agaaacaaac ggagaagctt ggagacagac
     1141 gatctattat ccatttatgc aagctgctgt gtatggacaa ggagaggtgt taagcccaca
     1201 aattcgttca gcaaactatg cgacggaaga ttttgaagaa gtgccgtatt tagaaagtat
     1261 agccgtaata aatgacaaag aacttgtcat atttgcagtt aaccgtggag aagaagaaat
     1321 gaatctaact gttcacttgt ctgaattatc attagaggga gtgattgatt tttctgaaat
     1381 gagtggattt gatacaaaag caactaatct tcctaaaagt gaacaagtaa aaccaagtca
     1441 ttcaacaaat gtaaaagctg aagatgggaa aatcaattgt ttattgaaac cgctatcttg
     1501 gaatgtggtc agatgtcgtt tatcagatga aggaggaaca aaaaatgaaa ggtaaaaaat
     1561 ggttaattgg ctctttgttg gtagctgcaa caggtatttt aggagcttgc ggtggcggaa
     1621 gttcagcaga atctgattct gataaagaag tgactttgac tttttggaat gggtttactg
     1681 catctgatgg tgaaatattg caagaaatcg ttaatgaatt taataaaaca aatgataaaa
     1741 acatcaaaat tgaaatggat atcatgactt gggccaattt gaatgaaaaa cttcctacag
     1801 cgatttcttc taaaaaagca cctgacttta ttgcattaaa ttatccagat tttgcacagt
     1861 atgtagaaaa cggagcggta caaccattag acgatttctg ggaatatgat ggagtagata
     1921 aatctgattt taaagaaacg gctattgaat taggtgaagt agaaggtaaa cagtattttg
     1981 tccccatgca ggtacaaggc atgtatatgt attggaataa tgatttgttt aaacaagctg
     2041 gattagatac tgaacaacca cctactactt gggaagaatt gacagaaatg gcaccaaaat
     2101 taactgatag tagtaaaaat gtatcaggtt ttgtcttcaa taaggatgga actgcaccgt
     2161 tatataactg gatgttagca aatggcggta aattagtgaa cgatgattat actaagtctg
     2221 aatttgcgtc tcctgagaac cttaaaacgt taaaagcgat ccaaaaaatg atccatgtag
     2281 acaagactgg tccagaatcg atttctggcg ccgaaatgga caatttaatg aatgctggtc
     2341 aactagccat tgaaatcaat gggccatggt taaataacgg attgaaagca aacgagattg
     2401 attataatgt gactacctta ccacaaggga gtgattcaga caaaaaaaca gctattttag
     2461 atggcgtagg atatgccatt ccggcaagta cagataataa taaaaaagaa gctgtctatg
     2521 agtttttgaa atattggaat acaactgaaa ttggtaaaaa atggagtatc gaaaatggtt
     2581 ttcctgccta cttggattca gtagcaaaag atgaagaagt aaaaaatgat cctattgtat
     2641 ctgaattatc gaaacaaatg gactatgcag agccatattt accaggtttt acccaaatct
     2701 ctgcgatcaa taacgatatc atcaatccac tgattgaaaa attattagca ggagaagatc
     2761 ctgagacact catgaacgat gcagatgatg ccatcaatca attactgcaa aaatagtaca
     2821 gaactttata caggatagac tgttggcaag atacagatat ctgaatgatc gacctagtat
     2881 ttgcagatag gtgttattgc aaaaatcaca gcgattgttc ggatatctta ttcagattcg
     2941 tttaaaccaa gattgtatat tttatcaaaa tgaaagatga ggtggactat atgaacggat
     3001 tcaccaaata ttggaatc
//
LOCUS       DACDHJ010000219         3015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDHJ010000219 DACDHJ010000000
VERSION     DACDHJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.223"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..509
                     /locus_tag="IV223_002371"
     CDS             <1..509
                     /locus_tag="IV223_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8712621.1"
                     /translation="LNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINY
                     GEIYSTAACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVE
                     DFTKKFSNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVR
                     RYMWQMKY"
     gene            491..952
                     /locus_tag="IV223_002372"
     CDS             491..952
                     /locus_tag="IV223_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8712622.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1002..1466
                     /locus_tag="IV223_002373"
     CDS             1002..1466
                     /locus_tag="IV223_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8712623.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1480..2229
                     /locus_tag="IV223_002374"
     CDS             1480..2229
                     /locus_tag="IV223_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8712624.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2229..>3015
                     /locus_tag="IV223_002375"
     CDS             2229..>3015
                     /locus_tag="IV223_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8712625.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLG"
BASE COUNT         1001 a          409 c          586 g         1019 t
ORIGIN      
        1 gcttgaatga atttgataag ttaaatgatc ttgttattgc tgaaaagagc aaatttgaaa
       61 taactgctaa agaatggggt aaaaaccaaa aacgagataa aattatttac acaattggta
      121 gcggtatcaa ttacggagaa atttactcca cagcagcttg ctggttcatg gaaatgcaat
      181 ggattaattc taatgctatt cattctgggg aatttttcca tggtcctttt gaaatcactg
      241 attatgatgt accattcatc ttagtgaaaa gtattggaga tacaagattt ttagatcaac
      301 gggtagaaga cttcactaaa aaattcagta ataaattaat ggttttagac caagctgatt
      361 ttgacttaga atctatagat agtgtagcga aagaatatat tgcagctgta cttagtggcg
      421 ttgttattcg ccaaatggta gaagcgattg cttttgaaag aggccattcc ttagatgtta
      481 gacgctatat gtggcaaatg aaatattaat taataggagg ttgcaaaaga tggcaaatca
      541 attatttcac attgtgatta caacacattc taccctttgt gagggttatt taaaggcaac
      601 aaaattaatt ttaatgaatg acgccacagg tattagtgct gttccattta aagaaaatac
      661 tagtccagat gattttgagg agagtatgaa agaaataatt agtcagcatc atttgcaacc
      721 tctaattatt ttaacggatt taattggagg aacacctaat aatgtttcta ttaaacagtt
      781 gaacaacaat agaattcaaa ttattgctgg gattaattta cctcttttac tggagttatt
      841 aatggcccag tcagctggta aatctataga agaaatagat tttaaggaat tactgaataa
      901 ctctcaaaaa agtatggttc atattaattc gcttttaaaa gatgtcagtt aaggtggcaa
      961 tttctattct ataaatttta aaaattgaag gaggaaatag aatgattaaa ttagtgcgtg
     1021 tagaccacag attacttcat ggacaagtaa tattttcttg gactaaacag tatgatatta
     1081 accatattat tgtggctgat aacaaagttc caggagatcc aatgtctgtg atggctttga
     1141 caattgctaa acctgctgat tgcaaattag acattgttaa aattagtgat gttaaaggga
     1201 ttatggaaac atccccaaat gataattaca tgattttaat taaaggacct aaggaagctt
     1261 ttgaattagt tcaaaattta ccagaaatta aagaaattaa ttttggcggt gtgtctaaga
     1321 aaagcgagtc caaacaatat ggaaaggcag tctttttaac tcctgaagaa ttagaagcca
     1381 caaaaaaaat tcttgatcaa gaagtgaaaa tatttgtgca acaagtccca tcttcaagta
     1441 ttgaaaatgt agattttagt aagtaagggg ggaaaataaa tgttattaaa agccatttta
     1501 ttaggtttag ttggtgtggt agctgttatt gactccaggt taattggtcg acaaaatatt
     1561 ggtagaccac taattttgag tacacttgcc ggacttgtgc taggtgactt gcatacaggg
     1621 attgttcttg gggcatcttt agaattaatt tctatgggat tcgtctcaat tggggcggct
     1681 ggacctccta atatgcaact tggtagtatt attgccactg cttttgcaat tttaactaat
     1741 agtagtacag aaacagcctt aacaatagct atcccagtgg ctgtagctgg tgaatttatg
     1801 agtattatca tgcgcatgat aattgctcaa tttggacatg ctgcagataa agccattgaa
     1861 aataataaat ttagaaaagc tcaaattatt catatttatt ggtcatttat ttttaatgct
     1921 tttgtttact ttattccaat ctttttaaca gtatattttg gtgctgacgt tgtggcagat
     1981 ttagtagata aaattccaga aatgattaca gacgccttga ctgtctctgg aaatatgctt
     2041 tccgcattag ggtttgcaat gctattaagc acaatgttga gtaaaaaatt atatccttac
     2101 tttatttttg gattctttat tgttgcctat tcaggtcttt cactaattgg cgtgacaatt
     2161 tttgcagctc tgattgcctt tgtaatggat caagttaaat atggaaaaag agaagaggcg
     2221 catggttaat ggaaaatagt acagaaaaaa aacgtttaat cacaaaaaaa gatcttaata
     2281 agatgtgtct tcgttcatgg acaatcatgt gttcgtggaa ttatgaaaga caaatgcata
     2341 tgggatttat gtatgggatg gcgccaattt taaataaagt ttataaaaat gatgaagaga
     2401 aaaaaagaga agcttatact cgtcatatgg cattttttaa ctgtacgcct caaatgactt
     2461 catttattat gggactttgt gcttctatgg aagaacaaaa tgctcaagca gaagaaggaa
     2521 actttaatac tgaatctatc tccatgatta aaaccagttt aatgggtcct tttgctggaa
     2581 ttggtgattc atttttccaa ggtactttaa gaattattac atttgggatt ggattgtctt
     2641 ttgctaagca aggtagtatt ttaggaccga ttttagctgt tggccttttt gctatacctt
     2701 ctattttagc tgttttttac ggcggtaaat taggatatga gtctggcaat aaatacctta
     2761 ctaaattgta tcaagaaggt cttatggatc gagtaatgca atttgcttct atcattggtt
     2821 tggctgtagt aggtggaatg gtggctagca tggtgccaat tgtcacccct ttaaaattca
     2881 ctacaggggg aacagaactt gttttacaag acttacttga ttccattatt cctggattat
     2941 taccacttgt atgtactttg ggaatatata aattgattaa gaaaaatgtt agtacaaata
     3001 tgctcttatt aggtt
//
LOCUS       DACDHJ010000220         3000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDHJ010000220 DACDHJ010000000
VERSION     DACDHJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.224"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(203..1513)
                     /locus_tag="IV223_002376"
     CDS             complement(203..1513)
                     /locus_tag="IV223_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8712626.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1525..2943)
                     /locus_tag="IV223_002377"
     CDS             complement(1525..2943)
                     /locus_tag="IV223_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8712627.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          937 a          621 c          468 g          974 t
ORIGIN      
        1 aagttttttt cgatagaatc aagcttcgca agtggttctt tttaacgact tagttatctt
       61 atcacgtcta aaaacatgtg tcaactcttt tttggaattt tcataaaatg tttcaatgaa
      121 gcgcgacaac gttgtttaat atatcattaa aaaaaataaa ggtcaagcta aaatattatt
      181 taagcttgac cttattataa tttcaacgat tgtcaaaagt attgtttgca gctgtgttgc
      241 cagtttctat agataaatca cagcttttga aacataagtt ttgtactgct tcgattcgcc
      301 cgaattcttt tgcagttatt tggcaatgag aaaagcagac atcttctata ggcttttcag
      361 gaaatgctac taaatcaaaa gctcgggaag gaagtgagta acctggagaa aggcatgcct
      421 gtacgttttt gatgtgtatg tctttgactt tcgtcatttg ctttgattca ggtatttctt
      481 cagccactgc ttgccaactt tctggcattt gagcaaggtc attgctattt tttctattgt
      541 attcattatg ccagtccatt atccaagaaa aaggaaattg tacatcaatc atattcagat
      601 tttctaccct gatattttcg atcactcccc ctctttcttt tgaagatttc attcgaatac
      661 cacagtctga ctgaaaaaaa tcgatatcgt gaatatatac gtcactaact ccagcactaa
      721 cttcgctacc aatagttaca ccgtatcctg aacggatttt acatctagca acctcgattc
      781 ttgatgattt tcgattgact cttgcaccat tgccatctct gccagactta atagcaatac
      841 aatcatcacc acaggccaaa tcacattcat agacacgcac atttgttgaa gaatcaatat
      901 caatcccatc tgtacttggt ccatcattgt tgcggatgat tactttttct actaatacat
      961 cgttagaata agtgatttgc aagttccaaa aacctgattt ctgaaaaatc aaatcacgaa
     1021 caacaacatg ttctgcatga tacagtaaac aagcgcgtgg acgtttaata gcataatccg
     1081 caatccaacg aagtcccttt tgatcgtatt cagctcttgt cccgcttttt tgatcttttc
     1141 cccaatacag ctcccaccaa tggtctcctt gaccgtcgat tattcctttt ccttctataa
     1201 gaatatcttt cgccgagaga acattcaaaa tcgctgctgg ccatttcatt tctacgccgg
     1261 ctactcgtgt atcgatttct ggaaatgctt cgatatctgt tctccccttg aggactgcac
     1321 cttcttcaaa gataagatga ctcttatctt ttaaaaaaag ggctccgact aaataaatgc
     1381 cttttggaat aaatacaggt aagtgttctt tcgcaccttg atcgattgct ttttgcagta
     1441 ttattgtatc atcttgttga gtgtcgtttg ttttaaagtt caaagcattg ataatccctt
     1501 tttttgtccg caatacgttt ctccctatct ttcattgatt tttttcaatt cttcaccaat
     1561 aaatacagtt tttgaacgat tcagtgggta acctactagc aaaatgattg cggatacaaa
     1621 taagcagaat gctggtaaca agtttgccat cgtataaatc ctttcttgta ctaaaactga
     1681 ttggatggct ccacctgttg tagacgattg gtaaccaatg agtgccagca taaatccgcc
     1741 taccgctcct gcacatgctt gtcctacttt tctcgcaaaa gaatttactc catatactgt
     1801 tccatcttct cttaagcctg tcttatattg atgataatca atcacgtcag taatgaatgc
     1861 ccagaccatc aaattgaata aaccagatcc taaagtagcg ataaataaaa gaatcagata
     1921 tacccatgca tttgttatat gaaggaaata catcagcaag tacatacatg aagaagcaag
     1981 taacgcaaat acgcttgctt cctttttacc aaataatttt gtcagctttg tagcaaatgg
     2041 tgctaaaact actactgttc cgtaagtaaa taaaagagca acagacattg cttgtttatt
     2101 atggaagtaa tcattgaata aataagtcat attcgtccca gctaaaatct gattgatcac
     2161 aatgaaaatg tcaacaatga ctaaaataat cagtgctcga ttagctaaca aaccttttac
     2221 caactgagaa gcagatactt tttcgttttt gacaactctt actctttcag tagtcaattt
     2281 acaagttaac atatatgcga caaatgctat tgcagcacat ccgattgaaa tccaaaagaa
     2341 atgattccct gaaacgactt gttgtccact cttgttatgg gtatacatta aaatcggaat
     2401 gaagaatcct gtagttgatc cgccaatcgc agaaccaatg cttcggaacg tggacaacga
     2461 ggtgcggtca ttaggatttg agctaattgc tgctgccatt gctccatacg ggatattgat
     2521 tgttgagaga aaaatcccat aaacaatgta tgaaacaaaa atataagcaa tcttcaccgt
     2581 cattggaaga tgttggacga atggcaaaaa caaaatgaca gtcactaagc aaaatggata
     2641 cttcattttt ctgatccaag gaataaatcg gccagtttct gttaactctg agacatcaca
     2701 taatcttcca accatcacat cagcaaacgc atccaaaatt ctcgaaatca agaataacgt
     2761 acctacgatt gcccctgaaa cgccaagaac attcgtataa taaatcatca agaaactatt
     2821 gactaaacct aaaataaaac agttacctag atcaccgaac atgtagccta tcttatcgga
     2881 aatcccaaat tccttttttt ctttttttat tgtatttgct gtaacagcat tttgtgaaag
     2941 catttgaatt cctcctacct cttctagtaa acgttttcaa aattataact ctttttttct
//
LOCUS       DACDHJ010000221         3000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDHJ010000221 DACDHJ010000000
VERSION     DACDHJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.225"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(471..1427)
                     /locus_tag="IV223_002378"
     CDS             complement(471..1427)
                     /locus_tag="IV223_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712628.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1536..1781)
                     /locus_tag="IV223_002379"
                     /pseudo
     CDS             complement(1536..1781)
                     /locus_tag="IV223_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1926..2348)
                     /locus_tag="IV223_002380"
     CDS             complement(1926..2348)
                     /locus_tag="IV223_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712629.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2487..2917)
                     /locus_tag="IV223_002381"
                     /pseudo
     CDS             complement(2487..2917)
                     /locus_tag="IV223_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          899 a          669 c          500 g          932 t
ORIGIN      
        1 taagactgat aaaaatattc tatcggttct ttttgcaaat aaaaaaacat atagaagctc
       61 cattagattg aatcaccaca acacaatcaa aggagcattc cgtatgcttg attttcattc
      121 tataaaacta cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata
      181 tccgaagtac cattgtttca tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt
      241 cttatatcgc tctgtcagtc ctattaataa agtactgttt tgttttcaaa aaaactatta
      301 cgagtacttt ttaagagaat gccttgcaaa tattcatcac acaccgttat ataagtcaaa
      361 tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca gttaagtagc
      421 aacttaattc tcggacttgt tctactctag caaaatttat tttcatcaac ttacgtccaa
      481 atgttttttt gaataattac ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc
      541 actagctttt ggcgtccttc aggatttcta gtatacacca actgacattt gggcagcgac
      601 gattgattta attcgtttag aaaaaggtta gcgatttgct tatttcggcc aaagcgttct
      661 ggtaccgtca gaactatctt tttcccttcg attaaatacc gcggattctc cagcggtgct
      721 aaaagctccc tcatagcttc agtaaaaagc tgtccttctc gatagccagc attctcaaga
      781 tagcatctaa attcctgctg atcagccgct acgattactt ttctatcact aacttcacca
      841 aggcgctgca acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc acattccttt
      901 tcccatagct tttgtcgcct acgaatttgg aaatattcca gcaagttacc tttaaatata
      961 gctgaccaga agcttagagg ctctatattt tttactacta tcggcaatga agtttcttgt
     1021 ttttgcttta cctgatcgac tttcctaacg acgattggca tagataggtg ccagccatca
     1081 gctaaagcag tactccattg ttctttcaac cgacttcgtt cacaagaaaa atagaacatc
     1141 gactgagtat aagtttccat cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg
     1201 ccaccactct caatttttgg tgaagattca aaggtcagac ctgaaatatt gcccaaacgc
     1261 cgtagcattc ttttcaaatc aggctgttca gacagaggaa ctcccggtaa tccaatcgct
     1321 aaatgccaaa tgttcgttac tttattttgt gcacctaggc gtaagccccg ccctcgtagt
     1381 tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga tcaacagatt aattgcagga
     1441 caatcccagc cttctcctag caaactaacg gttcctacca aaatattcaa cttcttttcg
     1501 gttaataggc gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa
     1561 taaaaataac ccgacatata aggatgttca ttcatacccg tcgctagttc tgccagctca
     1621 acagttacta gcattttcgg taacagtaag aactctccac agatggcacc gatttgctga
     1681 gtgttaccta atagacgaac aacttcatca aacaaaggaa ccacgccata agctaaggta
     1741 ccatcactgc ctgctaatac ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt
     1801 tcttgcttca gctgaacttc ttcttttaac aaaatttgac aaactgcttg ctttttctca
     1861 ggaatcgcct gactaccaac agtgtctgga tactctggaa acaaatgcag cgactcatca
     1921 aacaatcatc cagctttttt caattgataa aaaataggca attccggttg atcttcaaac
     1981 atctgctgat aaatagtgat cagctctttt ttacctaaag atggaactga agctttttta
     2041 ccttgctgct tagaactgcc taatagctgc caatgttcgg gtatcaattc atagttatga
     2101 tgattcaagt aaattaatgc actagtgaac agctcaaaat tttcataaat ttccttcgaa
     2161 tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat aatccaccgt ctcttggctt
     2221 tccattaacg cttccaccag ctcattttgt ttctctaaaa aggtaaaata atgctgagta
     2281 gtttcctgat tagccggaat caaatataga aaatcctgat gaggacatag aacctcctcc
     2341 tttaccaact cgggaatgct aatttcttcg tcaatcaggc cattttaact caatatattg
     2401 cttccactcc gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag
     2461 attcggttgt tttttcttca agtcaatcaa caattcaccc cagctttttt ttaaatgatg
     2521 agcttcatca aaaaactaac agctccagct gatcaccata agttggctcc tccttcagtc
     2581 ggttgtaaat ggtctgatac gtttccactg taatatgact aggatgttca atcgccccag
     2641 aaagatagca actatctgct ggatttacta agaaatattc ctcgattgtt gttaaccact
     2701 gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca
     2761 tctcaattcc taaaaaggtc ttgccagccc ctggtggagc cactagatgg actgaattgt
     2821 tatttaaata agtagcaaag ttagtcaata tacgcttctg atagctacgc cactgaattt
     2881 tttcatcaga tagccacatc ccaggtcccc ctctcacgta ttttctctat cataatctaa
     2941 atgaatacag caaaaaaaag atttcttcaa gaatatgtgt ttttatagta atattttact
//
LOCUS       DACDHJ010000222         2976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDHJ010000222 DACDHJ010000000
VERSION     DACDHJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.226"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(555..1121)
                     /locus_tag="IV223_002382"
     CDS             complement(555..1121)
                     /locus_tag="IV223_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8712630.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1121..2077)
                     /locus_tag="IV223_002383"
     CDS             complement(1121..2077)
                     /locus_tag="IV223_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8712631.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGNAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2077..2733)
                     /locus_tag="IV223_002384"
     CDS             complement(2077..2733)
                     /locus_tag="IV223_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8712632.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          937 a          582 c          533 g          924 t
ORIGIN      
        1 gaaaaagcta atatagctaa aattgaaaca tccatctgga atttatcttt tttactgctt
       61 tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc ttccatcata
      121 aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga tatttgaggg
      181 gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg aatgacatac
      241 aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt cttttatata
      301 tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa agaggttgtg
      361 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt
      421 tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa taaagaggtt
      481 atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt
      541 tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt
      601 tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa
      661 gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc
      721 aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct
      781 ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct
      841 ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat
      901 gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt
      961 tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc
     1021 gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct
     1081 tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc
     1141 gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca
     1201 agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcattcc
     1261 ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat
     1321 tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc
     1381 tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa
     1441 tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca
     1501 aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt
     1561 gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa
     1621 gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg
     1681 gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt
     1741 gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat
     1801 aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga
     1861 attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc
     1921 aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat
     1981 cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat
     2041 gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga
     2101 gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag
     2161 actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct
     2221 tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct
     2281 tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc
     2341 tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg
     2401 ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc
     2461 agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca
     2521 agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct
     2581 gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag
     2641 ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct
     2701 accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt
     2761 attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa tgtatgattc
     2821 ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag acattttttg
     2881 gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc ttttaaacaa
     2941 gaacgtagac gtaggaagaa cttgaaaatg aacaga
//
LOCUS       DACDHJ010000223         2966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDHJ010000223 DACDHJ010000000
VERSION     DACDHJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.227"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..242
                     /locus_tag="IV223_002385"
     CDS             <1..242
                     /locus_tag="IV223_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712633.1"
                     /translation="CVELSNRTGNKWSRNDYLKLLVENDFDRPLMEYKQEKFDQLLEK
                     FSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            244..666
                     /locus_tag="IV223_002386"
     CDS             244..666
                     /locus_tag="IV223_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712634.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            685..2277
                     /locus_tag="IV223_002387"
     CDS             685..2277
                     /locus_tag="IV223_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712635.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2298..2633
                     /locus_tag="IV223_002388"
     CDS             2298..2633
                     /locus_tag="IV223_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712636.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2779..>2966
                     /locus_tag="IV223_002389"
     CDS             2779..>2966
                     /locus_tag="IV223_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712637.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYP"
BASE COUNT         1173 a          443 c          593 g          757 t
ORIGIN      
        1 gatgcgttga actaagtaat cgtacaggaa ataaatggag tcgtaatgat tatttaaagc
       61 tactcgttga aaatgatttt gatcgtcctt tgatggaata caaacaagaa aagtttgacc
      121 agttattaga aaagttttca gaaattcaaa gttataacac aaagatatta gaagaatatg
      181 ttagccaaaa taatcgtatt atagaaatct tgattgagca aaatagagga agtgaacttt
      241 gatatggcaa aggttataga caaacacatt tactttcacc aaacacatat cgatgtattg
      301 gatgaaatta tttctactca tgcagaggtt aaaaactata gtgaagctgt tcgcttttta
      361 tgtatgaact atgatccaga agtggagaaa aaagtaaatt ccaatcaagc aaagttgaat
      421 ggaatgagta aagaaatttc tattttaacc gaaatggttt cagaatttgc ggatcttcat
      481 ttgaaagata caggaatatt aattggtaaa aattcaactg tttatcaaga agcaaaagaa
      541 cgagtggaag gaaaaattaa atccaatcaa acaaaaaaat attcccctca gttgaagaaa
      601 cctgtgaatg aatctgttca tgccagagta gatccatttg atccaacgaa gattctaaaa
      661 agataaaaat gaaggaggat aataatgact caatcgcctt cagttattct cacttgtcaa
      721 tttactttgc cgaataaaaa gtcattttct acttatattg actatattac aagggaacag
      781 gctcttgaag aaaaggaacg acgtacgccc gaagaagaac aggaacttca acgaatcaag
      841 aatgctttag aggagtttta tattcctgaa ggagaaactt tttcggagag taagacgggt
      901 aaagaattca gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt
      961 gacaagttag aggaacaaga ttttaccaat tatctttctt atatgacacg tcactatgcg
     1021 ttagaacaaa agaaagaact gacgcaaagt gaacaaaaag aataccaacg cttgaaccaa
     1081 gcaatgaaaa aatatcaaac agaaccccat aaaaaagctt caggaaatct tcctggagtt
     1141 ttttctatag cttcagacga agtgaaagga agtgatctga aggagattaa gcaaatcttc
     1201 caaagaggac aacaaaaagg ctcaatcatt tatcaagatg ttgtttctca tgataattcg
     1261 tatcttgaaa aaataggatt gtacgatccc aaaacagata ctttagatga agaagggtta
     1321 aaagcagctg gaagaaaaat gatggagaca ttattcgaga aagagcaact gaacgaaaca
     1381 ggctactgga tggcaacgat tcaccgtaac actaaacata tccatattca ttttgcagtt
     1441 gttgaaaaag aaaatacgcg taaaccttac acggaggttg aaaatggtgt gagttatatc
     1501 gtaccgaaag gtaagagaag acaagcaaca ttagacgcaa tgaagaccaa ttatgtacat
     1561 gagttagaac gtttttctta tgaaaaggat cgaatgattg gtatggagaa acgaaattta
     1621 ttgacgcgga aaagtgattt gcgtaacacc ttaacaaaag tggtaaaaga gccagcgcgt
     1681 tacgatcgac acgcgatgaa cttattaaat gaagtatata aaaccctacc tgacaaacgt
     1741 tcggaatgga attacgggga tgaaaaacga accaaacttt caccatacac aagagagaaa
     1801 ctcgatgagt taaccaaata tgttttaaca aaagaacctg aatatcaaga atatatacag
     1861 atttcaaaag cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca
     1921 aaggatgcag agaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccatccta
     1981 acagagctga agagacagga tagtcaaata aaaccattaa tagaaagtgt tgatcagatg
     2041 caatctattt acaatgaacg atcctttcga acgcttaaac aattgccaac gaatgaatac
     2101 aaatatagtt acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg
     2161 cagcggagtt tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca
     2221 agtaaagcat acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaaaga
     2281 cagtaggagg aatagcaatg aaacaatatt tagatcaatg gaaagttata gaaggatcat
     2341 taagagaaga acgaatcgaa caacttcctg attgtttaga aaaagagcat ttgttccaga
     2401 taagagaaat gttgagaaac gaacagtttg atccaaatca atttttagtc gttgagtacc
     2461 cagctactgg tgtgtattgt tgtaatcatg tgaatggtga aaaatatttt atcattcaag
     2521 aatatgaggg caagttagca ccttattata ctacttggga aatgaatgaa gagggaatca
     2581 ataactttcc atgtaaaagc attgaagaat ccatttcact cacagagtgt tagttgttaa
     2641 gtgaataaaa gaagtaaaag agtcctgaac gtgattgtcc aggactcttt ttttcaatga
     2701 attgttttcc gccaactcat tgagtggtgc gatttgcgac tacattatat caaaagaaag
     2761 agaagggagg aagcagaaat ggttcattca acgattggag ttgttgttga aaaaagcaga
     2821 gacaatctcg tttttgtgac agaaattcaa acaggcagat cttttgtcgt cacagacaaa
     2881 gcggcaaaag cttatcaaag tagcgatatt ttaacgttaa atctgactac gaagacgttc
     2941 gtcgatgcga cagaagatta tccatt
//
LOCUS       DACDHJ010000224         2964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDHJ010000224 DACDHJ010000000
VERSION     DACDHJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.228"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(194..808)
                     /locus_tag="IV223_002390"
     CDS             complement(194..808)
                     /locus_tag="IV223_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8712638.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1414..1791)
                     /locus_tag="IV223_002391"
     CDS             complement(1414..1791)
                     /locus_tag="IV223_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712639.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2091..>2964)
                     /gene="galU"
                     /locus_tag="IV223_002392"
     CDS             complement(2091..>2964)
                     /gene="galU"
                     /locus_tag="IV223_002392"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8712640.1"
                     /translation="KKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEALDS
                     GIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQSHP
                     RGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRVPEN
                     ETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILETQEP
                     GAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGTSLS
                     AYIIQLSKKLRTES"
BASE COUNT          901 a          618 c          467 g          978 t
ORIGIN      
        1 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
       61 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
      121 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
      181 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
      241 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
      301 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
      361 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
      421 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
      481 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
      541 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
      601 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
      661 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
      721 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
      781 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
      841 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
      901 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
      961 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
     1021 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
     1081 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
     1141 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
     1201 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
     1261 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
     1321 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
     1381 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
     1441 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
     1501 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
     1561 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
     1621 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
     1681 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
     1741 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
     1801 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
     1861 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
     1921 tagcagaata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
     1981 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
     2041 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
     2101 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
     2161 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
     2221 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
     2281 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
     2341 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
     2401 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
     2461 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
     2521 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
     2581 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
     2641 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
     2701 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
     2761 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
     2821 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
     2881 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
     2941 gcggcaggaa tgactgcttt tttg
//
LOCUS       DACDHJ010000225         2917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDHJ010000225 DACDHJ010000000
VERSION     DACDHJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.229"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..766
                     /locus_tag="IV223_002393"
     CDS             <1..766
                     /locus_tag="IV223_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8712641.1"
                     /translation="FPPFDYAKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEIL
                     QRTYSSNANVFVQLLNVDIPNYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSN
                     AIVKSVASGEGVIHALNTMRLDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEK
                     SYLETGIEEYNVLTAKDERVCRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPN
                     LPKLGKDIDEEYDRLFGDLLDEFAHDSFGINLKRRK"
     gene            768..1085
                     /locus_tag="IV223_002394"
     CDS             768..1085
                     /locus_tag="IV223_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712642.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            1134..1835
                     /locus_tag="IV223_002395"
     CDS             1134..1835
                     /locus_tag="IV223_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8712643.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            1850..2740
                     /locus_tag="IV223_002396"
     CDS             1850..2740
                     /locus_tag="IV223_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP8712644.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            2781..>2917
                     /locus_tag="IV223_002397"
     CDS             2781..>2917
                     /locus_tag="IV223_002397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712645.1"
                     /translation="MVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITADSNNEFI
                     IS"
BASE COUNT         1081 a          410 c          584 g          842 t
ORIGIN      
        1 ttttccacca tttgactatg caaaagtcaa tcgcttatta caaatacgat cagatgtatt
       61 taaaattctt gcagaatatg cgatggatgc agatgtgaat caatattttt ctgatcgctt
      121 agaggaaatt cttcaaagaa catattcttc taatgctaat gtttttgttc aacttttaaa
      181 cgttgatata ccaaattatt taccagaaaa tgaaattaaa cattatttga actatccatg
      241 gtcaggaaag acattttcaa gaagactttg gggaaatatt tcatcgcttg aacagaaact
      301 ctctaatgct attgtaaaaa gtgttgctag tggagaaggc gttatacacg cattaaacac
      361 catgagatta gactcagaaa tttgtgacat gtttaagtta gaagaatcaa agtataacaa
      421 agcgatagaa aatctcgttc gaacggagta tgcaaaattt gcacaagatg gtattgaaaa
      481 atcatattta gaaacaggta ttgaggaata caacgtattg actgcaaaag atgagagagt
      541 ttgccggatt tgtggaggaa aggcaagtaa gaatccctat aaactgaaag atgctgtcat
      601 aggtgaaaat cgagcacctt tccatagtcg ttgtagatgt acggatgttc ctaatttacc
      661 aaaattagga aaggatattg atgaagaata tgaccgttta tttggcgatt tattagatga
      721 gtttgcacat gattcttttg gaattaattt gaaacggagg aagtagaatg aaagattttt
      781 ttgaagcagt actaacaatt aatgtaaatg ctgatattgc agaagcctac aaaacagcta
      841 ttgagtctga gaaccatcct aatggcttga gagaccattg gaatggcaat tatgcctacg
      901 tggttattgg cgatcaaact gttaattatc aagataatac tccagttgat aagaataccg
      961 ttaatttaac gattcaatta ttatctcatt cattaccaaa tttaaaagaa acagttgatt
     1021 ggtatgaaaa gatgggatgt attgttgtta gaactgacta caaagaagga aagtctagta
     1081 attaggcttt tttattttgt ccgaaatgac gttaaactag cgcaatactg ggcttggttg
     1141 aatggtgggg cgcaactatt aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg
     1201 gcgaaaggag aatgagcatg aaacacaaat cattaatgcc aatgaatttg caatactttg
     1261 ccgaaggtga cgatcaaaag ttttcttttg atgacttcaa atcttttgtg gaatccaatg
     1321 aagaagctca aaaatttatt cagtcacaat ctcaaagtgt tgccgacaaa caattggaag
     1381 cttggaaaca aaataactta gataaaatta aacaggatac catcaaggaa tatgaggaat
     1441 ctaagaaaaa caagtcacct gagcaaattc aactggaaaa actacaagct gaatttgaag
     1501 cagaaaaagc gttgcgtgtg acaagtgata ataaagcatt tgttgcagaa caaattgctg
     1561 gattagaact agatggagag ttaaaagagt ctatttctca atttatgcta aataatcttg
     1621 ttagttcgga tacagatttc actaagaatg ctgttgaagg ttttacaagt gttttgaatg
     1681 caattaatga gaaacatgca gatgcattaa aagaactaca aatgaagtct gcatttggtg
     1741 gaactcaaca atcgaataac caggttcagc agaacaatga aacattaaca aatccagaag
     1801 aacaattagg acaaattctt caacaattta actaggagag tgaaaaatta tgaaaaaaac
     1861 atctttaaat aatttagagt atttggatat ttcaccagcg attaatgcta tgcaagtacc
     1921 aaatacacct tttttaagct atttacttgg tgctggaaaa acagagccag caaactcgac
     1981 agaaattaaa tggcgtgaat atgatatcaa caacgatgat tcttctgaaa aacttgaggg
     2041 cggagaatat ccagatgctg aatcaggtcg aacttggttt aacaactata ctgaaatttt
     2101 tagaaaatca acctctgtat ctggtacatt agatgctatt aatgtgaatg gagtcggaaa
     2161 tgaattaact aatcaagtag cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa
     2221 attgattact ggtgtaaaag ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat
     2281 tttgaacttg atcaattcag caaataaggc agaaacagcc actgcgggtg cagtaacaag
     2341 aaaagatatc gatactttat ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact
     2401 atgcttgatt tctccagata tgcaggagtt aatgactgat gagttagatg gaaaatcaac
     2461 aaaaattgtt cagttcgggg aaagagtaac ttttggattg caaattggaa atatcgtgtc
     2521 taattacggt acaggtattg ctctactaga accatcattg ccaaaaggaa caattgccgc
     2581 aatcgatact aattatgtga aactacgtcc attacgtgaa tggagagcag aagaacttgc
     2641 aaaaacaacg gattctagac gtatcggtct tgtaggtgaa tactctcttg aatacaatgc
     2701 ttcaaactct ggggcaattt taaatttaaa gtctgaataa aagggagtta gtactccctt
     2761 ttttgatagg aggaattaca gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga
     2821 aatatagagt aggtaaaact aaaaattttg ttgggtttgt tcatcctaaa actcgtagat
     2881 ttatcacagc agattcaaat aacgaattta tcatttc
//
LOCUS       DACDHJ010000226         2880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDHJ010000226 DACDHJ010000000
VERSION     DACDHJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.230"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..2198
                     /locus_tag="IV223_002398"
     CDS             138..2198
                     /locus_tag="IV223_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712646.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2281..2850
                     /locus_tag="IV223_002399"
     CDS             2281..2850
                     /locus_tag="IV223_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712647.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1038 a          519 c          553 g          770 t
ORIGIN      
        1 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
       61 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
      121 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
      181 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
      241 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
      301 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
      361 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
      421 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
      481 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
      541 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
      601 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
      661 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
      721 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
      781 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
      841 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
      901 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
      961 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     1021 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     1081 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     1141 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     1201 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     1261 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     1321 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     1381 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     1441 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     1501 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     1561 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     1621 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     1681 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag
     1741 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     1801 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     1861 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     1921 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     1981 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     2041 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     2101 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     2161 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     2221 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     2281 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     2341 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     2401 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     2461 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     2521 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     2581 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     2641 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     2701 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     2761 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     2821 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
//
LOCUS       DACDHJ010000227         2853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDHJ010000227 DACDHJ010000000
VERSION     DACDHJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.231"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..380
                     /locus_tag="IV223_002400"
     CDS             15..380
                     /locus_tag="IV223_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712648.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            397..1008
                     /locus_tag="IV223_002401"
     CDS             397..1008
                     /locus_tag="IV223_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP8712649.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            1036..2697
                     /locus_tag="IV223_002402"
     CDS             1036..2697
                     /locus_tag="IV223_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8712650.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            2725..>2853
                     /locus_tag="IV223_002403"
     CDS             2725..>2853
                     /locus_tag="IV223_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8712651.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVS"
BASE COUNT          910 a          441 c          605 g          897 t
ORIGIN      
        1 aggtggtgaa gttaatgact gcagaagtat gtacaactac ttgtatacat aaagaaaaag
       61 tagctagtgt taaaaataat ttaaaagata aagacctttc aactttactt gtattaggaa
      121 aatgttttag cgattcttct aggattaaaa ttttctatgc tttagaaacg tataaggaga
      181 tgtgcgtttg tgatttagca gcgatactta atgctagtat tgctacaacg tcacaccatt
      241 tacgtttttt gaaaaagaac ggaatggcga aatcacgtca atatggaaag gtagtttatt
      301 actctttagc gaatgaagag attctttccg ctgtgcaggc ttttctaaag ttgtcagaaa
      361 atagttctat gaaagcttag tttggaggta gttattttgt tacaaaacat tttatcagca
      421 ctcgctgtat atatttctac cagtattgat tatttattta tcttgctgat tattttttct
      481 caaaaccata ctaaaaaagg tctccgtcag attttttttg gtcaatatct tggaacagga
      541 atcttggtag ccataagttt gtttgcagca tatgtgctaa attttattcc tcaagattgg
      601 atcattggtc ttctgggact aattccgatt tatttaggga ttcgtgttgc ttttgtgggc
      661 gaagaggagg aagaagaagg ggaagtcgtt gagaagctag gatctagagg aacgaatcgt
      721 ttgttttgga cagtttcctt aataacgatt gcttctggtg gcgataattt agggatttat
      781 attcctttct ttacctcgtt atctttttca gaaattgtca cttctttaat cgtctttgct
      841 atctctgttg cggttctttg ctatattagt tataaatttg cgaaaatttc ttttgtctct
      901 gaaacactag aaaaatatga acgaatcatt gtacctatcg tatttattgg attagggatt
      961 tttattatga tagaaaatgg aaccatccaa actatattag gtttgtagag atcttattag
     1021 taaaggaagc aaacgatgag tgaaaataaa aagtaccatg aatggatatt agaaggaatc
     1081 gactgtgcaa attgtgcaga caaagtagaa agaggcgtcg ccaaagttgc aggtgttgcc
     1141 aatagtagtg tgaactatat gacagaaacc ttgagtttcg aagtgacaga ggaaaaagaa
     1201 tatcttgttt tatccaatgt gcaacaaaaa gtaaaaaaat tggagcctga tatcacttta
     1261 aaaaataaag tggacggtag gctgattgaa ataaatccgg cattaatcaa gaaaagtaat
     1321 ttaaaagacg ataaaaaaaa ctacgaatgg atattagaag gaatcgattg tgcaaactgt
     1381 gctgccaaag tggaaagagg agttgctaca gttcccggcg ttacaaacag cagcgtcaat
     1441 tttatgacag agacactgag cttcgaggta gcaggtgaaa ataaaactga cgtgttggct
     1501 agtgtaaaac aaacagtagg aactttagaa cctgacgttg ttctgcgaaa aaaatcggac
     1561 ggtacattga ttgaacatgg aactgactct aataaaaatt cgacttctaa caaatcgatt
     1621 tctgataaag atgagacaaa ccaaaagcag acgaatagag cttatctggc cagccttctc
     1681 ggaaataaaa ctaaaattac ccttattcgt ttaatactag gattaggaat attagcgaca
     1741 ggaatatttc tccctatgga tggacaagcc tctcttgggt tgttcattct ggcttattta
     1801 atcaccggtt atgacgttgt aggacgtgcc attagaaata ttttccgagg tcaagtattc
     1861 gacgagaact ttctaatgac cattgcgacg ttaagtgcct tctatattca agagtatccc
     1921 gaagctgtag cagtcatgct attttatcaa gtgggagaag tattccaaga tattgctatt
     1981 tcaaaatctc ggcgctccat tgcggaccta atggatattc gacctgatta tgccaattta
     2041 gtcacaggaa acaacgtaga aaaagttgct cctgaagcaa ttaagattgg aaatattatt
     2101 ctcattcgtc cgggcgaaaa agtgccattg gatggaaaag tagtggaagg tgcttctgcg
     2161 atggacactt ctgctttaac aggcgaatcc gttccaagaa gcgtgaagcc ggaagacact
     2221 attttgagcg gatttattaa taaaaacgga ttgctaaaag tagttgtaga aaagcccttt
     2281 tcggagtcta ctgtgaagaa aattttagat ttggtccaaa atgctagtgg ccggaaagca
     2341 cctactgaac aattcattac caaattttct cgctactata ctccaattgt ggttatagct
     2401 gctgccttac tggcaattgt tccgccattg ctagtaccag gcgctacttt taatgaatgg
     2461 atatataggg ccagtatctt tttagttatt tcatgccctt gtgctttagt cgtttcgatt
     2521 ccagttggtt tctttggtgg gattggttct gcttcccgca aggggatttt agtaaaagaa
     2581 agcaatttcc tcgagggttt gaacgatctt aaatacgtgg taatggacaa aacgggtaca
     2641 ttgacaaaag ggttctgttg caaagtttta aatctactat caaataaggt agaataatag
     2701 aaaaagatag taggaggaat gacgatgaat cattttaaag gaaagcaatt tcagcaggat
     2761 gtgattattg tagccgtggg atactatctt cgttataacc ttagctatcg tgaagttcaa
     2821 gaaatcttat atgatcgtgg cattaacgtt tct
//
LOCUS       DACDHJ010000228         2851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDHJ010000228 DACDHJ010000000
VERSION     DACDHJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.232"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1077
                     /locus_tag="IV223_002404"
     CDS             <1..1077
                     /locus_tag="IV223_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712652.1"
                     /translation="TFEERQQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQ
                     KIKERYEKKRDNAYSLCVAKINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTD
                     KLKISKELNEQLNKLYQTTTEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEK
                     QRQKEKEREQLELKKREQDLAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQL
                     NYLAQPQPTPSVAAVQTPMNNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNH
                     INQSSQADSVATTTSIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSK
                     AKEGSSDSTVQSNTSESSESNEVK"
     gene            1077..>2851
                     /locus_tag="IV223_002405"
     CDS             1077..>2851
                     /locus_tag="IV223_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8712653.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHR"
BASE COUNT         1096 a          472 c          555 g          728 t
ORIGIN      
        1 acctttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc gacggaacag
       61 tcgtctaatt gggagaaagc aaaagaggaa ctaaataaag aagaacagcg agtgattgaa
      121 gaagaagtac aaaaaattaa agagcgatac gaaaagaaac gcgacaatgc gtattccttg
      181 tgtgtagcaa agatcaataa ttttactcag acgaatcaag aagcgttatc gaaagaaaaa
      241 gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag aattttaccg aggtttgcgt
      301 acagataagc taaagattag caaagaatta aatgaacaac tgaataagct atatcaaaca
      361 acgactgaag ttcttatgga aaaatacgaa aagatccagc aagaattaca agtgcaaatt
      421 ccaaaatgga aaaaagagta tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa
      481 caacggcaaa aagaaaaaga gcgcgaacaa ttagaactaa aaaaacgtga acaggatctt
      541 gcggaacaac aaatggcaat agaaaaacaa cggttaaacc aagaaaagga aaaagaagca
      601 gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagctca gttaaattat
      661 ctagcacaac ctcaaccaac gccttcagta gctgctgttc aaaccccaat gaacaaccaa
      721 acaaatctaa ctccttcacc acagcgttcc tttaacggat ggatggtggg atgtattgca
      781 agtttaagct tgcttctagg tggaggaaca gtatttgctt ttaatcatat caatcaatct
      841 tcacaggctg attctgtggc cacaacgacg tcaatcaagg aagaagccca agaacaagca
      901 agaaaagagt atgaacaaca attagaagaa ttattgaatc gtaccaatca gtcaacaaca
      961 gaatcaagta tggaaaatag caaagagagt gatcctagta aggctaaaga aggatcaagc
     1021 gattcaactg tacaatcaaa cacttccgaa agttcagaat caaatgaggt gaaataatgc
     1081 gtgttagaga aacaacgaga cgaaaagaca aacgattcaa tttagaaaag aataggtatc
     1141 tttctcattt ctatcgctat actccttcag gagaagagct accagaaaaa catgacaaac
     1201 gtatttctca aaagattttt tatagtggct tagcttatct tcctttaaac tatgttgtta
     1261 caagaatacc agaagcattt catcgatttg agattattcc aggaagttta caaggagtga
     1321 atatttttca gttacaagaa atgccgcagt tattccattt tgattcaact ggtttttgga
     1381 atcctttgac cccaacaaca agtttaggca cacgtttagt tgtttctacg atttgtgttg
     1441 ttccagctgt acttatggga ttgaaaatgg acatgtggtt tcgtcctcta gcagatgggc
     1501 aaaaaggaga taaccgatta aaaacgttaa aggaaatcca acaaatttat ccgcaaatac
     1561 cagaatcaaa gggaggtttt cctggttatg gcggaatacc aattactcat tataaaaagt
     1621 actggtatat tcagacggat acagtaaata cttgtattgt tggtacttcc cgttcaggga
     1681 aaggacagat agaagtttta gcaaccattg acaatttgtc tcgtgcagaa agacaaagtt
     1741 caatggttgt taatgatcca aaaacagaat tatatgttgc tagtaaagat gaactagaag
     1801 ctagagggta tgatgtgtat gcatttaata tcaccgatcc actgcaatca atgagtgata
     1861 atccattggc actcatcgtg aaatactgga agcgaggaga catagacaca gcgacacagt
     1921 taaccaatac tttcacgaat acgatttatt atgatgccaa cgcttcagac aataaatact
     1981 ttaacgaaaa tgcgcaaaaa gctgtcaatg cgcttatttt tgcgttattg gaacaagcgg
     2041 atcagacagg ggattactcc aaattaacac cgaataatct cgtcgaactt ctttctgaga
     2101 ttggtggttt taattaccaa gatcctgata atgaattcaa gcagctcaat gctttagatg
     2161 aatttatgaa tcagttacca cctggaaaca tagcaaaaaa acaatatggg gcaacaaaaa
     2221 ttggttctga taaagcgaaa ggcaacattc tatcgacagc gatcacagga ctaaatccat
     2281 ttacactgac gaaaattgcg aaaatgactt ctcaatcaac cattgattat aagtccattg
     2341 gttttccgaa gtatttggat ctcaaactac acgagagtct gttaaaccag cgaattgttg
     2401 tagagtttta tcatcaagga aaaaaatttc atgaggaacg tgtcaaagta ggctatagag
     2461 gtttttgtga aattaacttt gattgtcatt tagaacaggg agatcatgtt tgcttaaaat
     2521 acgatcatcg aggaaaagaa tatgttgctt ggtatgagtt ttctcaaaga gtattgaaag
     2581 acgaagaggg aaatatcgtt tatcgaaaaa aacgaggaga aacccatttg ccagaattag
     2641 aaaaagaagc cgttctaaca ttagatgagg ataaaagtaa cttattagta gagtctattc
     2701 ggatgcacta taccgataaa ccaatggcta tttttatgct cacacctgat tatgatccat
     2761 ctaaccatat cattgttagt atttttctta gtcagttgta taaagaatta tctacgcaat
     2821 gtgttaagac taaaggagat aaatgtcacc g
//
LOCUS       DACDHJ010000229         2845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDHJ010000229 DACDHJ010000000
VERSION     DACDHJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.233"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1068
                     /locus_tag="IV223_002406"
     CDS             <1..1068
                     /locus_tag="IV223_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712654.1"
                     /translation="TFEERQQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQ
                     KIKERYEKKRDNAYSLCVEKINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTD
                     KLRISKELNEQLNKLYQTTTEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEK
                     QRKKEKEREQLELKKREQDLAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQL
                     NYLTHPQPVAAVQTPVNNQANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQ
                     SSQADSVATTASIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKE
                     GSSDSTVQSNTSESSESNEVK"
     gene            1068..>2845
                     /locus_tag="IV223_002407"
     CDS             1068..>2845
                     /locus_tag="IV223_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8712655.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHR"
BASE COUNT         1103 a          461 c          557 g          724 t
ORIGIN      
        1 acttttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc gacggaacag
       61 tcgtcgaatt gggagaaagc aaaagaggaa ctaaataaag aagaacagcg agtgattgaa
      121 gaagaagtac aaaaaattaa agagcggtac gaaaagaaac gtgacaatgc gtattccttg
      181 tgtgtagaaa agatcaacaa ttttactcag acgaatcaag aagcattatc gaaagaaaaa
      241 gaaaacttat taaaagaacg tataaaagaa cgaaaagaag aattttaccg agggttgcgt
      301 actgataagc taaggattag caaagaatta aatgaacaac tgaataaact ctatcaaact
      361 acgactgaag ttctgatgga aaaacacgag cagatccaac aagaattaca agcacaaatt
      421 ccaaaatgga aaaaagagta tgaaattcac caacaagaat tgaaagaaca agaagaaaaa
      481 caacggaaaa aagaaaaaga gcgcgaacaa ttagaactaa aaaaacgtga acaggatctt
      541 gcggaacaaa aaatggcgat agaaaaacaa cggctagaac aagaaaagga aaaagaagca
      601 gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagcaca gttaaattat
      661 ctgacacatc ctcaaccagt agctgctgtt caaacgccag tgaacaacca agcaaattta
      721 actccttcac cccagcgttc ctttaacgga tggatggtgg gatgtattgc aagtttaagc
      781 ttgcttctag gtggaggaac agtatttgct tttaatcata tcaatcaatc ttcacaggct
      841 gattctgtag ccacaacggc gtcaatcaag gaagaagccc aggaacaagc aagaaaagag
      901 tatgaacaac aattagaaga attattgaat cgtaccaatc agtcaacaac agagtcaagc
      961 atggaaaata gcaaagagag tgattctagt aaggctaaag aaggatcaag cgattcaact
     1021 gtacaatcaa acacttccga aagttcagaa tcaaatgagg tgaaataatg cgtgttagag
     1081 aaacaacgaa gcgaaaagac aaacgattca atttagaaaa gaataggtat ctttctcatt
     1141 tctatcgcta tactccctca ggagaagagc taccagaaaa acatgacaaa cgcatttcga
     1201 aaaagatttt ttatagtgga cttgtatacc tccctttaga gtatttagta acaagagtac
     1261 cagaagtttt caaccgattg gaaattgtta agggaagctt acaaggaata aatctgtttc
     1321 aattacagga aatgccacag gtctttcatt ttgattcagc tggtttttgg aatccattta
     1381 ctcctacaac tagtttgggt acacgtttag caatttcgac agtttgtgtg gttccagctg
     1441 tacttatggg attgaaaatg gacatgtggt ttcgtcctct agcagatgga caaaaagggg
     1501 ataatcgatt aatgacctta aaagaaatcc aacagattta tccgcaaata cctgaaacaa
     1561 aaactacttt tccaggttat ggaggtattc caatcacaca ttacaaaaaa tattggtaca
     1621 ttcaaacaga tactgttaat acgtgtattg ttggtacctc tcgatcaggg aaaggtcaga
     1681 ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga aaaacaaagt tcaatggtcg
     1741 ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga tgaactagaa gctagagggt
     1801 atgatgtata tgcctttaat atcaccgatc cactgcaatc aatgagtgat aatccattgg
     1861 cactcatcgt gaaatactgg aaacgaggag acatagatac tgcaacacag ttaaccaata
     1921 ctttcacaaa tacgatttat tatgatgcca acgcttcaga taacaaatac tttaacgaaa
     1981 acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt agaacaagct gatcaaacag
     2041 gagattactc caaattgaca ccgaataatc tcgttgagct tctttctgag attggcggtt
     2101 ttaattatta tccagatcca gacaatgaat tcaaacagtt gaatgcctta gatgaattta
     2161 tgaatcagtt gccacctgga aacattgcaa aaaaacaata tggggcaacg aaaattggat
     2221 ctgataaagc gaagggaaat attttatcaa cggcaatcac gggattgaat ccatttactc
     2281 taacgaaaat tgcaaagatg acttctcaat caacgattga ttataaatcg gttgggtttc
     2341 caaaatactt agatttgaaa ttacatgaaa gtcttttaaa tcaacgagtc gtagtagaat
     2401 tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa agtaggctat aaaggattta
     2461 gtgaaatcaa ttttgattgt catttagttg caggtgatca agtttgttta aaatacgatc
     2521 atcaaggaaa agaatatgtg gcttggtatg aattttctca aagagtattg aaagacgaag
     2581 agggtaatat cgtttatcgg aaaaaacgag gagaaaccca tttgccagaa ttagaaaaag
     2641 aagccgttct aacattagat gaggaaaaaa gtaacttatt agtagagtct attcggatgc
     2701 actataccga taaaccaatg gctattttta tgcttacacc tgattatgat ccatctaacc
     2761 atatcattgt tagtattttt cttagtcagt tatataaaga attatctacg caatgtgtta
     2821 agactaaagg agataaatgt caccg
//
LOCUS       DACDHJ010000230         2793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDHJ010000230 DACDHJ010000000
VERSION     DACDHJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.234"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(430..876)
                     /locus_tag="IV223_002408"
     CDS             complement(430..876)
                     /locus_tag="IV223_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8712656.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            1471..1821
                     /locus_tag="IV223_002409"
     CDS             1471..1821
                     /locus_tag="IV223_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712657.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            2026..>2793
                     /locus_tag="IV223_002410"
     CDS             2026..>2793
                     /locus_tag="IV223_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8712658.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
BASE COUNT          952 a          554 c          436 g          851 t
ORIGIN      
        1 ccttttctgc ggcctcattg atagctggaa tatatacaga ttctagtcga tagataaaaa
       61 cagattaagt acgaggtttc caatgaatta taaaatccaa taaaactcag taaaatgtga
      121 tttaaaagta tgttcaagac cactcaccag taagtttaat ctatttagca aaagaagcta
      181 tgtctacact ctttaaaatc aatatgtttt ctcattttta aagtttcaaa aaaactctat
      241 aactaagaaa aatcacaaaa aagagtacat tttctagtga aactgcaccc ttttttgctt
      301 tgtttacgta caaaaaatct cttgagtact taacttctac agtataatca acaacccctt
      361 tatagattac tgatgttatg ctgaaaaaaa ttttttcacc aacttgtaat ctaagccact
      421 tttcttattt tacttctcaa accaatcata aaattcatct ggtgttttct cccgatagag
      481 tttgattaac gctggtcgca tcgattgaaa tatttcgact aattcttccc caccaaatcg
      541 agtggaaaaa tgcttatcag taaatatcat tgataaagaa gaaagaaaca gctccgtatc
      601 ttcatctgta ccaagaatat tgatcaactt tccaatcaaa taggaatcat taaatgtcag
      661 gtcttgtttc acatcaaagg tttttattga ttctttcacc attttcgcaa tgcgatcttt
      721 ctctccatac ttgatctcac cactcatctc gatatagtca atgatcccag atgaaagaat
      781 cgtgacagct tcttcttttg cttttgatga tagtttgttt agcgttttgg attcttcaag
      841 tacctctttc gtaatctgta cgacatagtc cttcatgatt tcactcgaat attctacaaa
      901 acattcttgg ataaattcct ccaaaggata gcctagctca ttctcccaag caggattttt
      961 gaactcgccc gtataaaatt cttgctttat tttctcaatg atttcaggat aatcgtccac
     1021 tatttttttc tgaccaatga gaaacaaata ctctcggata tattcttcca catgcccaaa
     1081 ataaaaccag ccgacaggtt ttccgctgat actagataat tggttgatca cactcttagg
     1141 agccaacgca tagccttgaa cgtaggaact gatcgtcggc ttttttaaac ctaatcgatt
     1201 ccctaattct gtaaaagaga gagacattcc gtcttttatt tctttgattc gttggcccac
     1261 cttcacttta ttaggttgta ctaaaccaaa catattcaaa ttcctcctta tcattttttt
     1321 cattataaca ctcgtataaa aaatcgtata tattttttta gaaaatctct tgactacgta
     1381 taagttctcg tatataatac aagtcgtaga tggccatcta tattttttaa tcaaatcgta
     1441 taaactttcg tataaaaaag gagaattgtg atgccaataa aaattaccga tctcagttta
     1501 ccaaaagaaa cactcgaaaa agaataccaa gtcgtcaatg tcactcgctg gcaaaaggag
     1561 ggcgaaattc taggttggag ctacgaatgt atcctaccaa aacttcgcta cgaaaaaatc
     1621 agtgtcaaag tcaaatctga acatcctgtg atcacgccgg aagaacttac ccaacaaggt
     1681 ttagccataa ttacatttac caatttggtg attgtacctt ggggacgctc gaatggacaa
     1741 tttgtcagtt atgggttatc ggcaaccgct gatacggcaa ctttgattcc cagaaaacaa
     1801 ggtaccacta cacaaaacta actatactat ggagtgttgg gtggttagga cgaaacggcg
     1861 gggcagcgcc cacagagccg tttctgtccg ttaagaccgc ccaacacccc ataggctaga
     1921 agggctgcta cgcaggcctt cccccttaac ttgatttata gatcaacatc aggagaaaat
     1981 gactggttct ttgacaatcg aatacgcaac aaggagtgat gatttttgca agcttataat
     2041 cagaaaatca ttgaaactcc cacgtatatt gaaatttatg attatgaagc aatcattact
     2101 cccaaacgta aaacgatcga aaacgaagta aatctggaac gaaaacaaaa attagcttgg
     2161 atttatgaaa atgatattac tcttgaaaaa agaaaaaact atgatgaatt atccgctcaa
     2221 ggacaatatg acagtttaaa acgcaaacag aaacattatc aaagtatgcg atttgagatt
     2281 gcccgccttg tagacacgaa ttttgacaat caaacgaaat ttttaactct cactttcaaa
     2341 gaaaacattc aagacattga gtatgcaaat aatgaattta aaaaatttat caaacgactg
     2401 aattatgacc tctataaaac taaaaaagca aatttaaagt atctcgccac gtgggaaaag
     2461 cagcaacgcg gtgcaatcca ctaccatatt atcttgtttg atttcccctt tatctcattt
     2521 aaccggttaa ctacgatttg gggtcagggt ttcgttaaga tcaacaagat cgatgtagac
     2581 agtatcgaaa atcgcgggcg ctatatcagc aaatatttcg ataaagagct ggaattgaaa
     2641 gagcataaga aaaaagcttt tttcaagtcc cggaatctca aactaccaac tgtcaccaaa
     2701 aagatggtaa agaaacccta tgattccacg aatcaagata tactatatac aagcgaatat
     2761 atagttagac gtccattttt ttacaatgct tta
//
LOCUS       DACDHJ010000231         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDHJ010000231 DACDHJ010000000
VERSION     DACDHJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.235"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..1624
                     /locus_tag="IV223_002411"
     CDS             8..1624
                     /locus_tag="IV223_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8712659.1"
                     /translation="MPELSQKEVEIPHFDRASLRENGQKNPVWIHFGGGNLYRGFHAE
                     IAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEFVKRILAATAD
                     SLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGPIAVDIKNGPK
                     KPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAKEWQKEGYVQN
                     TFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTAKGNNIAPFVN
                     TEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPLHTSLAIMGCL
                     LGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVITVRFSNPNIPN
                     MPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRYLIGIDDQGKP
                     FIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLYQAELGRKIEE
                     MFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1615..2697
                     /gene="uxuA"
                     /locus_tag="IV223_002412"
     CDS             1615..2697
                     /gene="uxuA"
                     /locus_tag="IV223_002412"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8712660.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT          921 a          489 c          584 g          790 t
ORIGIN      
        1 gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg accgtgcatc
       61 attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg gaaatcttta
      121 tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat tgtccactgg
      181 tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc aagattttga
      241 tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac gtatattagc
      301 tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg ataagatgat
      361 tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg aaaaaggtta
      421 tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata taaaaaatgg
      481 tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata aacgtttttt
      541 atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa acggtagtca
      601 attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa
      661 tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga cgatgattga
      721 tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc
      781 aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg tcaatacaga
      841 ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga aattcgaaaa
      901 agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga tgaaggtgac
      961 gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac tggggtatag
     1021 gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg aaatcgggta
     1081 caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga tctttctgga
     1141 agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc agcgaatcgc
     1201 cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa aatatatgga
     1261 aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg ctgcttggct
     1321 tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc cagacccact
     1381 attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag atacatcaca
     1441 tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg tagatcttta
     1501 ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag caggaccaaa
     1561 tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt tatcatggaa
     1621 atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga aatcagacag
     1681 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
     1741 cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
     1801 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
     1861 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
     1921 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
     1981 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
     2041 gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga acctgagcgc
     2101 ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
     2161 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
     2221 atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg aatcgtaaag
     2281 aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa cggaatcact
     2341 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
     2401 ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
     2461 cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat gtaccgcata
     2521 ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca tggaagaatg
     2581 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
     2641 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
     2701 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga tacataaatc
     2761 cggaggttgt taataagcat tcgg
//
LOCUS       DACDHJ010000232         2781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDHJ010000232 DACDHJ010000000
VERSION     DACDHJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.236"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..583)
                     /gene="eda"
                     /locus_tag="IV223_002413"
     CDS             complement(<1..583)
                     /gene="eda"
                     /locus_tag="IV223_002413"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8712661.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGD"
     gene            complement(667..2094)
                     /gene="uxaC"
                     /locus_tag="IV223_002414"
     CDS             complement(667..2094)
                     /gene="uxaC"
                     /locus_tag="IV223_002414"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8712662.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(2168..>2781)
                     /gene="uxuA"
                     /locus_tag="IV223_002415"
     CDS             complement(2168..>2781)
                     /gene="uxuA"
                     /locus_tag="IV223_002415"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8712663.1"
                     /translation="GWEPHRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVG
                     IKMAVHPDDPPYSIFGLPRIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYE
                     ILSEFCQRDRIPFAHVRNIKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGY
                     IRPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEALEKGASK"
BASE COUNT          855 a          598 c          389 g          939 t
ORIGIN      
        1 aatcaccttt ttcaacagga gccaacaaat tcccacctac accaactgca acaacgcctg
       61 cttcaaacca ctcttctaag ttatctagac ttactccgcc agtaggcatt atatttacat
      121 atggtaatgg agcttgatac gtttttacaa cagatggacc aaaagcagaa cctggaaata
      181 gtttaatgat ttctactcca gcggtaagtg cctcctttat ctcagtaatt gtcatacaac
      241 ctggtaaata aggaatttga tataaattac aaatctctgc tgtttgtcga tcaaaagatg
      301 gacttacgat atattctgct ccagccataa ttgcaatacg agcagtaaac gcatctaaaa
      361 ctgtaccggc tccaattatt acatcttttt gatcagcata ttttttagca atttctttga
      421 ttatttcttc agcattcgga actgtaaaag ttagttcaat ccctttaatt ccacccgcag
      481 taattgcatt tattgcttta atggcctcat ctttattttt tccacgtaca accgcaatga
      541 ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc tccttctttt
      601 atggatacat actctagcta gttgtactaa cttacaataa ataaatttaa gcttttattt
      661 tttgtattat tttctacttt cgatttcaaa aaaattaaaa taattatacg cattacgata
      721 acaaatattc tccactatct gactaagata catttcatca tctggaattc gacctgcttt
      781 aacccactcg cttagtaatt gacataatac tcgacggaaa tactcatggc gaggatagct
      841 tagaaaactt cgagaatcag tcaacatacc tgtaaaattc gacaacaaac tttgttgcgc
      901 catcgtagtc aactgttgac gcattccttc agctgtatca ttgaaccacc aagcacatcc
      961 caattggatt ttttgaatca tacccccttg gaagctttgc attgctgttg ctagtggaag
     1021 ccaatcggtt ggatttaatg aatataaaat tgtttttggt aagcacccac tcttttcagc
     1081 agtgtcgaac aatgctacaa cattttgtgt caaactagct tgatccccca tcgaatcacc
     1141 accgacatct actccaattt gttcctttaa tatacttgaa ttatttctta aagcatttaa
     1201 atgcatttgc atcgtccaac catatcttgt atactcagac atcaaatgta attgaatacc
     1261 tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt tttcctgcta aagcttttat
     1321 taacagattg tcgagtattt tttcatctgc tatttcataa aaataagtat tcaaaccgtg
     1381 atctgctaat cgacagccaa tttgatgaaa ataatccact cgttggctca atgcctgctt
     1441 caacccatta aagtttttga ttggtatacc cgctgcttga ctaagtttta aaatatagtt
     1501 cgaaaatcct gatgatttga tactccagac cttatctgga cggaaagtag gtaatacctt
     1561 gaaagaatta tcctgtttag aaagctcagt atgatatcgc aagtcatctg taggatcatc
     1621 cgttgtacaa atggctttca catccatcat cttcagcaat tcttttgggc gataggaatc
     1681 cgtttggatt ttttcgttgg cacgtcgcca aatttctggt gcattcttct cagaaattgt
     1741 caaatcaata tcaaagtaac gttgtaattc caaatgagac cattcaaaaa taggattacc
     1801 tggcgccttc tccactgttt ttacaaaagc taaaaatttt tcataatcat tacctgcacc
     1861 actgatcaac tcttcttcta tcccattagc tcgcatcaat cgccatttat aatgatctcc
     1921 agctccttta tcatttaacc atacctgagt caaattcttg tattgcttat tttctagcaa
     1981 acatgctggg tctaaatgac aatgataatc gatgattggt tggtgttctg caaatttatg
     2041 aaaaagtctt ttagctattt cattctctaa taaaaagttc ttatctaaaa acatatttaa
     2101 gcactccttt ccttccctta gtttttagaa gagaaaattt ttcttctaaa tagactccat
     2161 tatataatta tttactcgcc cccttttcaa gcgcttccca aataccattt aaataaactg
     2221 cacctagagc tcggtcatat aacccataac caggtctacc ggtttctccc caaatcatcc
     2281 gaccatgatc tggacgaata tagccattaa atccattatc gtacattgtt tttaatatct
     2341 tatacatatc tagagaacca tattctgatt tatgcggcgc ttcataaaaa tcgagtccat
     2401 tatcacttaa atatttgata ttacgaacat gtgcaaaagg aatccgatcc cgttgacaaa
     2461 attcagatag aatttcatag acatcattat ctggtcgttc tgcaatactt ccagtacata
     2521 acgtaatacc attagaagga gaatccacta cattacacaa ccattctaaa tcgtcacgat
     2581 ttttaacaat acgaggtaat ccgaaaatag aatatggtgg atcatctgga tgaactgcca
     2641 ttttaatccc aacttcttca catgtcggga taattgcttt gagaaaatat gcaaagttct
     2701 ctcgcaattt cgcttcatca acttctttat aggcttctaa taattgcttg atttgtgata
     2761 accgatgagg ttcccaaccc g
//
LOCUS       DACDHJ010000233         2780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDHJ010000233 DACDHJ010000000
VERSION     DACDHJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.237"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(46..408)
                     /locus_tag="IV223_002416"
     CDS             complement(46..408)
                     /locus_tag="IV223_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712664.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(482..862)
                     /locus_tag="IV223_002417"
     CDS             complement(482..862)
                     /locus_tag="IV223_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8712665.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            944..2119
                     /locus_tag="IV223_002418"
     CDS             944..2119
                     /locus_tag="IV223_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8712666.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2196..2780)
                     /locus_tag="IV223_002419"
     CDS             complement(2196..2780)
                     /locus_tag="IV223_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8712667.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          853 a          544 c          474 g          909 t
ORIGIN      
        1 aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct gtattttaga agaaaggcac
       61 tccctctact tcgtaagttc caagaacaga gcgcatttga cctggttctt cccaaatata
      121 tccttcgcct aattcttctc tttgattctt ttgttcagtt tcaaagacaa acaacggaac
      181 attttcctct ttaatatgtg tatttgtcaa ttccatcaaa ctaatcttgc taacatcgat
      241 atgaactatt tcttttaaga aattaagtat attcgctaaa ccagtctttc cttcagaaag
      301 ttccgctaac gcaaattcca acttgtcatc aattgaatgg accaaaaatt ttttagtacc
      361 atcctccaaa tgcaacataa ttgtaccagc aactcttttg cttgccaaaa tttccacctc
      421 gatcaacttg ttctatggct tacataaaaa ttctagcaca ttttcagaaa aaatacactt
      481 tttatttctt attttctttc cgttctagtt cagataatgc ttcttctatc caaataggta
      541 actgctccgc aatagtggta aatccgatct tacgattttt attcgtgaaa tacctttttc
      601 tgcgatatgg aacagctgga tttgtttttt ctaacgttct taaagataaa ctgatttttt
      661 tggaatattc atcaatgtcg atgatctgaa ctgttactgg ctgtccgact tttaacaatg
      721 aatggatatt tttcgtataa ccggattgta cttctgaaac atgaataagt ccttgcgtct
      781 cgttatcaag agaaacgaat gcgccataag gctgtatccc tgtcactttt ccttcaatta
      841 tttgtccaat tttataagtc atctacttcc ctctcttcgc atttatcatt atactgtaaa
      901 tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt aagatgatcg aatttgataa
      961 acagatttca agaaaagata cagaaagtgt aaaatgggac gcaattgctg agacatatca
     1021 aatggatgac ttacttcctt tgtgggtagc agatatggac ttcttgtctc ccggtggggt
     1081 agctaaagcg tttagtgaat acatcaaaca tggaatcttt ggttatgcca ctctgtctga
     1141 taaactatat caggctatca ttcattggga acgccaacac catgctgtgg agttgacgaa
     1201 agaaaatatc gtttttacaa gtggtgtgtt aaccagttta gctgcagcag tacaaacttt
     1261 tacaaaacca ggcgattcta ttttgattca tgacccagtt tatcctccat tttcctctat
     1321 tatagaaaca aatcaacgtc acatcgttcg aagctctttg ctagaaaaaa ataatcattt
     1381 catcatggac ttgtctgaca tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat
     1441 tttatgcaat cctcacaatc ctggagggcg tgtttggagc aaagaagaat tgaaacagtt
     1501 aagcgaattg tgtttaaaac atcaagtgat tctgtttagt gacgagatcc atcaagattt
     1561 aactttattc gatcatacat ttacttccat gctaacgatt gatcctaatt tagatgatct
     1621 actagttgct tttacttccg caaccaagac tttcaatcta gctgcaatca aaaattcaat
     1681 ggtatttatc aaaaatccag agatgaaagc tgattttgaa aaacatttag tgatgaacca
     1741 acagcacgaa atcaacactt ttggactgat tggaacccaa gcggcttatg agacgggtgg
     1801 agaatggtta agccaactga ttccttatct agaaaaaaat gttcaaacag ttaatcagtt
     1861 ttttgaacag tatcttccga aagtgagaat aatgaaccct gaaggaactt acttaatgtg
     1921 gttagatttt accgcctata tttcagacga ccaagaattg gagaacactc ttgttcggaa
     1981 aggaaaagtc gtattaaatc ctggcatcac gtttggtcct tccggtcatg ggcatatgcg
     2041 cttgaatatt gcttgtccag aagaaacttt actggaagga cttaatcgaa tcaagtatgc
     2101 tttagagaca atcgattaaa tcatttattc aaaataaaag gacgagacct tactaggttt
     2161 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2221 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2281 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2341 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2401 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2461 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2521 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2581 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2641 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2701 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     2761 tctagttgtg ggaatgccat
//
LOCUS       DACDHJ010000234         2748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDHJ010000234 DACDHJ010000000
VERSION     DACDHJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.238"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            189..2279
                     /locus_tag="IV223_002420"
     CDS             189..2279
                     /locus_tag="IV223_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8712668.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            2306..>2748
                     /locus_tag="IV223_002421"
     CDS             2306..>2748
                     /locus_tag="IV223_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712669.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKY"
BASE COUNT         1032 a          433 c          525 g          758 t
ORIGIN      
        1 ttattttcta aactatctat tttgccaagc taccaactat acctaatgag gattttaaac
       61 ataccagctg cttccatgcc gctagatatt atggaaaagc tcattaggaa tccattattt
      121 actacgatca atttgatcgt tattgtcgga atttcactag tcatttataa aaaagagggg
      181 atgaagggat gaattttaaa cactcgtgga caagacggtt aaaatatgtt tttcccataa
      241 tgatgatgtt aggtacattg tttgggttaa aaacagccaa tgtctttgct gaaaaagtta
      301 ttgtcgatcc gtcaaatcca atccagaacg taaagaataa ttttattatt ccaaaagatg
      361 ttcctaatgc aaaaacaaat attactgtaa acggagcgtc aaaagtttac aaatatcatg
      421 ttgatgccaa caaaggtggt tttacagacg actacgtagg attagtggag ggaaacaaaa
      481 atatacgcta tccttctttc caaaaagaat acggtgaaac aaaccttgtt ttagaaggcg
      541 taacaaccaa caccaaagtc aatattgact atggaaaaat cggtacgtat aacgggaaaa
      601 aagtcaatat caagctagtt ctatctaata ttcacttata ctcggatacg ttgccgtgga
      661 atctactaga taacaactat acaaaaactc attttcgaga cgatggctat aaaaatacga
      721 atggtgcaat gagtaaatct aaaaaaagaa cagtactttg gatctcagac aatcttttct
      781 caggaattgt ttaccattca acacaaatga atgtgcagct agtagccaca tatgaggacg
      841 gtagccccgt tcaattttca ggagatacat ttatcagttt taactctttg aaccctgcag
      901 gaggaaaaag cacggatctt aaaggagaat atgcccacta tgacaaaatg aatacgacag
      961 attggtatgt cagaaaagat acggttcttt cagaatttaa atctttctac aacaatctga
     1021 acgtggtagg tggccaccca ggaggaagct ctaagctgac acaagcggat aatgatttca
     1081 ataatttgca tgataaatta ggagatccga agtttggtca aggtactgtg tcctttaaaa
     1141 tatctgaagc aaatccgacg tttgtgattg gttcttctaa tgttcaaacc tggtttactt
     1201 tatcttctgc gacaatcttt agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata
     1261 aaaatggaaa taacgtgaac gataaaatgt tgcaagtagg agacacgatt caatatcgaa
     1321 tcaaacagaa ggtgaatcga ttaggagtgg atttactagc ggtttacgat cgatttgagt
     1381 taatcgataa tttaccaaaa gaagtaaatt acgtcgatgc acacgttgaa agtggtacaa
     1441 ataaaaaatt cgatgtatct ggtgaagtga cctatgacaa aacaaaacac caggtaaaat
     1501 atgcagcgaa agctgataca ttaaaaaatc gaatgaagta taacggcgaa acttacgaat
     1561 tagtgatcaa tgtaaaagtc aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg
     1621 gaacgtcgat cattaataaa gtagaaaagg atacaaatat cattaccgtt tatttcccta
     1681 aaattccagt gaaagaagta caacaaaatg gaaaagatgt aaacggacga aatgacggta
     1741 agaagggtac gccgacagct cctttaaacg caggatctga agtacaatat ctagtcacac
     1801 aaaaatggca cacaaaaggc gtagacgctg cgtcagatca ctacaaacaa tttagtattc
     1861 aagatccaat tgaagctcgt ttgacatata aagaaggttc tgcgcaagta atagataaga
     1921 gtaaaggaaa agatattact tctgaaggca cactaaccta tgatagcaat tcaagaacgc
     1981 tgaagtggga agcttccgct gacttcttaa gtaacaatct tttagatgga cgagaaattc
     2041 agctgatatt tacagctaaa actccattac aatcagaaaa aaatattgat aaccaagccg
     2101 tagtagcagt agataatgtt tctaataaaa caaatgttgt gacaattggg gttgatccta
     2161 acttgccaca ggtcattgtt cctaaaacag gttctacaca tttagtaacg atttcagcgg
     2221 tcagcttact attacttgtg ttagcaactt ttagttatgc ggttctgaga tacatttaaa
     2281 ataaaaaaac gaaagaatga ataaaatgaa aaagttaata atagtagtta cttccttgat
     2341 tgccatgatt gttattttag tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga
     2401 taatggcgta ttgaaatcag gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa
     2461 tttgaaaatt atagacgaga ctacctggga atactctgag agtgtatggc atccagatcc
     2521 tgtacaaata acagtcaaga ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt
     2581 agattcagct ggtgttaggg aatttataaa caaatctgat tctttattta ttgttgttcc
     2641 ctatgagaag aatggcgtaa aaaaaataat ttttcttgga tctagcaagg atgaaaagcg
     2701 aacgaaagag aaattgattc acgatggatc acaatccaat aaatataa
//
LOCUS       DACDHJ010000235         2746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDHJ010000235 DACDHJ010000000
VERSION     DACDHJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.239"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(151..1029)
                     /locus_tag="IV223_002422"
     CDS             complement(151..1029)
                     /locus_tag="IV223_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712670.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(1352..2377)
                     /locus_tag="IV223_002423"
     CDS             complement(1352..2377)
                     /locus_tag="IV223_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8712671.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          933 a          530 c          450 g          833 t
ORIGIN      
        1 acgaaaattg cttttcaaat ttttgtgaat ttcagcttat ttccgaagga gttgcttctg
       61 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtctcac
      121 gctcttttaa actatttcga agtactaaac ttatgaagta agaataccta agccagagcc
      181 aatgtgctcg cctgcaagca attgctcttt tgcttgtcgc tgtaaactcg tactttctgg
      241 ataatcatgt atttgggcat ctactcgtcc aaagatccaa gggactccac tatcgtcctg
      301 ttcatcaact gataataatc gataatcggc tgcaaaaaga cagatttttg actctttttt
      361 caaccattta gtaagattgg gtgatagtaa ccaagtatct gtgtacatca cttggtaatg
      421 gtaatcagga aaataaagtt caaaaaaacg aatggcactt tcgtaactat ctcttatctt
      481 attctgagat aaatctgcat cggaaggaat atgaagagaa aggcactgct ttccttctaa
      541 tcttagatcc gtgtaccctt tcggaaaata tgtcatttcg tactccaatt caccaatccg
      601 aaagatcaag ccactagtgt aatgccaaat ccaaaaacca cggtcaaagc gatagcttcc
      661 tgttacttgg aaatgttcat gaataaatct agtaaaagca ttcattgttg ccaaatagat
      721 agttcttgga atcttcaatg gttgataaat ttttttccaa ctttgagaag ctgcatatag
      781 ataaatagct aatgctgcaa aaggttcctc ttcatagact ttgtctattt tctttgcagt
      841 cgcctttgct ttattaggat ccattagttc atgactatat tgctcaaaca cctcatcaag
      901 gatgtttttt gcttgtacaa tcatttgctc tataacgact ttgggaagag ttaaagtttc
      961 tgccagaatt tggatttcat tgcttatttt cttcatcaaa tttccccttt tcatatcttt
     1021 gaacctcatt gtttaaatga ctgattatcc ggacaccata tggtataaac atacttttat
     1081 tgtacactaa ctataaaact taaactttct atgaagaaag aatatctgta agactttcaa
     1141 ggaaagcatt gaccatctca ggaaagttta ttcagttacc atcataaaca aaattttttg
     1201 atagaataaa tataaaattt ttttgaaagt tcctttccac atttttgtgt gaatttgact
     1261 taatgctgag aatctggctt ttgaacacct tgaattagag gttgtgacaa aaatcttgtc
     1321 ataacctctt ttggcacata tatttgtcct tctattctaa tttttgacga acaaccgtta
     1381 aataatgttg caaaccaacc gtcattaccc acgcaacacc taaaccgatg aaacttccta
     1441 tgacgatccc taaaaaacga tattcgatca aagaaatcaa gtatgctcca cgaataagtg
     1501 tagatagcat caacgccatc ggattaagaa aaatattagc aactgcataa tttctagcaa
     1561 ctagaaattg tgcgattcca tatagaaaag tgatcagaaa aatggtctgc aactgcgtaa
     1621 acggcacaaa tgacaagaat gccgacacac ataacccacc catcgatcca atcagatatt
     1681 ggacttgtcg atgcttcatc gcatccaagt tttctgctag caaaatcgat gcacatgaca
     1741 aggtcaacca ataaggatta tgcagattca agccgtgact gacataaacg gaaagaaata
     1801 aggcagcgct gtagaaaaca gaatcaatga tcactaaagg ctcttcgtga aatctttctt
     1861 ttaaagttgc tttttcaatt gtctgctcag gccgtgaatc cagtaatttt gtaatcactg
     1921 ccgcaatcag ggcaaaaacg acacccataa aaaaataaag tgagacgatc ggtaattgag
     1981 cgattggcag ctgcatgctc gttcccatgg cagaaagcat cgcaaaaaac aagccacctg
     2041 gtttagaaat atggaataat cgcaacacga atctgctgga aaaagcaacc aaagctacgg
     2101 caaatggttc aagccataaa acataagtcg ataagatccc caaaacgaaa gcaaccaaca
     2161 gtccgcaacc gatcagaaac atacgtttca gcaaatattt catcggtatg ttttgataat
     2221 agaaaaaagt aaagatccct aatgatgaaa aacttgaaat caaaaaatca tgatttatgt
     2281 aacctaacca taatacaata aagataagta gcatcagatt catgatacga aaaggattat
     2341 cttgtatttt attgaaacga aaataagtct tgaacaatct atccctcctt cctagtaatt
     2401 ggaaaaaaag ctgaaccaag atgaaatgcc aatcccgttt gtcagcatta cctagtataa
     2461 caactatttc ttgatttttc ataaacttag tatcaaaaag taaacaactt ggagaaatta
     2521 gaacaaaaaa cgaacattcc gagtcgtttt gacagaaaaa agatagcaac ttttccaagc
     2581 aactcgctat cttagctttg ttaaagaaac cggattccca acaaaaaaaa gcttctaatt
     2641 ttcgtacgat aactacgttg gtccttaaac ttcatacttt tttatgattt ttcagcggtt
     2701 ttcgatggta tactttttaa atggaggaga tgtctatgcg gtatgt
//
LOCUS       DACDHJ010000236         2729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDHJ010000236 DACDHJ010000000
VERSION     DACDHJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.240"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            143..1210
                     /gene="rlmN"
                     /locus_tag="IV223_002424"
     CDS             143..1210
                     /gene="rlmN"
                     /locus_tag="IV223_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8712672.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1403..2506)
                     /locus_tag="IV223_002425"
     CDS             complement(1403..2506)
                     /locus_tag="IV223_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8712673.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          866 a          488 c          549 g          826 t
ORIGIN      
        1 cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc
       61 gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt
      121 tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga
      181 attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga
      241 atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt
      301 gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga
      361 agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac
      421 tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa
      481 tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg
      541 agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga
      601 acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt
      661 aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat
      721 tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca
      781 agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg
      841 gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa
      901 aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga
      961 gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt
     1021 gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt
     1081 attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca
     1141 tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt
     1201 tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa
     1261 ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt
     1321 ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct
     1381 tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg
     1441 tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat
     1501 aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg
     1561 tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca
     1621 catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat
     1681 aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat
     1741 agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg
     1801 tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac
     1861 tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt
     1921 ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga
     1981 cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa
     2041 taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca
     2101 ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa
     2161 tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc
     2221 aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga
     2281 tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct
     2341 ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc
     2401 ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg
     2461 catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc atttccttct
     2521 ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg
     2581 gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa
     2641 tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa
     2701 tttttgttcc tcttagtatt tctaccgat
//
LOCUS       DACDHJ010000237         2713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDHJ010000237 DACDHJ010000000
VERSION     DACDHJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.241"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1095
                     /locus_tag="IV223_002426"
     CDS             <1..1095
                     /locus_tag="IV223_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8712674.1"
                     /translation="TVFVTLFLILAADSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIK
                     GRGPFSDEIAQYAVGAAVKYKLLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGC
                     LFPKGTARGIGGIEGGWYMKFPNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILG
                     RGGYAAAGITEDSPYYTGCMSIITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFP
                     GSSLDKSSFSGGQLFGTNPGGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNP
                     GISGLGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFT
                     RMDWRQAQGHAFTDDGTWIDPLPSLNSSKK"
     gene            1111..1755
                     /locus_tag="IV223_002427"
     CDS             1111..1755
                     /locus_tag="IV223_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712675.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            1766..2572
                     /locus_tag="IV223_002428"
     CDS             1766..2572
                     /locus_tag="IV223_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712676.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
BASE COUNT          988 a          439 c          599 g          687 t
ORIGIN      
        1 actgtgttcg tgaccctttt tctaatactt gcggcggatt cgattgataa ttctgattca
       61 gattcaagta gtggtggcga agcttttaca ggagaatatt cggaaggctt accgatttat
      121 aaagaaatca aaggaagagg ccctttttct gatgagattg cgcaatatgc tgtaggtgca
      181 gcagtgaaat ataaattatt accaagtgtg atcctttcgc aatacggtta tgaatcagcg
      241 tttggtacgt cggcgtccgc aagaaatgat ctcaactatt ttgggattac ttggtttgat
      301 ggttgtctgt ttcctaaagg aactgcgcga ggaatcggtg gtattgaagg tggctggtac
      361 atgaagtttc ccaactcaaa agcagccttc tcctattacg gctttatggt agccacacaa
      421 tccaatttta atgcatgtgt aggaaataaa agccctggcg caagcttatt gatcttagga
      481 cgaggtggtt atgccgctgc aggaattact gaagatagtc cgtattatac aggttgtatg
      541 tccattatca cttctaataa attaaccgaa tatgatgaat tcgccatcaa acattggggt
      601 gaaggtggca ataacaacgg cacgatcact ggtgaatgga cgaatccatt ccctggcagc
      661 agtttagata aaagttcctt cagtggtggt caattatttg gaacaaatcc aggcggtgaa
      721 ttccgtccga atggcttcca tgatggatta gactttggtt cagtcgatca tccaggaagt
      781 gaaattcacg cggtgcatgg aggaaaagtc gtgtacgttg gcaatcctgg tatttcaggt
      841 ttaggtgcat gtgtgatcgt gattaattac gacggtttga atatggtata tcaagaattt
      901 gcgaatagta caggaaattc tcgtgtgaag gttggcgatc aagtgaaagt tggtcaagta
      961 atcgggatac gagatacagc acacttgcat ttaggtttca ctcgaatgga ttggcgtcaa
     1021 gcacaaggac acgcattcac agacgatggt acatggattg atccattacc atctttgaat
     1081 agcagcaaaa aatagatcag gagaaaaaat atggaaaata aaactttaaa aatcgtctta
     1141 acagctgcaa ttagcgttag tttaatcggc aatatcgcgt tgttttggca gctgaaagaa
     1201 gaacaagaga caaatacctt taaaattgaa gaagtggcta aaaacgcgtc tgagaagctc
     1261 aaaaaaaatt cctctgaggc acaggcgaag atcgacaagt taaatcaaac catcaatgag
     1321 aaaaaccaag aaatcgatca gctgaagcaa caagatggca caaaagcaaa agaggaagta
     1381 actgaagcac aaagaaagac ggcagaagag ttcggtcaat tggccattga taaatcatta
     1441 agtagaaatg aattaacgga aaaactaaaa gatgtcgcaa cccaagaagt gatcgacaaa
     1501 ttaagtcctg cagacgatga tcaccaacat gatgattacg gaagttactc ctttacatta
     1561 ggtgacgtgc aaacttatgc tcaaacaagc agtgtaaaag aggaacaaaa ttttgtcgta
     1621 tttgttgatt atacaatcgg caatccgcaa ttcaaagatg tcaaaccaca aaaaataaaa
     1681 ggtggattaa ctgtcactga aaaacaagta gatggtcaat ggaaagtcat agactttagt
     1741 tatttcactc gttaggagga agaaaatgag taagaaaaaa ggagaaagac aacgtttcaa
     1801 aaaacgaaaa agttatcttc aatggctggg aatctttatg attctcggct ttttgattct
     1861 tgctagtccg atttatacga atcagaaggt agatatacca gatgaggtaa gcacgaacgc
     1921 ctttttaaac acgaaagagc tgacaatggt ttcaaaagaa aaaggagcga accagcaatt
     1981 agtttttgaa tttgcgatcg atgatgaaga tcaaagcttg ttaaaagaac tggctaatct
     2041 taattatcgc gtagaagtaa agacagctaa aggggattat caagcaatac gagccaaagt
     2101 gatcaaggtg tctgatgatt attttgcagt gaagctaacg catgtaccag aagaatatat
     2161 cgccatgcgt ttaaccatta ttcctgaaaa aattgatcca aaagtggata tgcaagaacc
     2221 gcaagatctg attttttata ttcacgaaga taaggtcaaa gatcaagtga aagatgaaga
     2281 ttatgaacaa cacgcgatca attacaaagt gaaaggatac aagaaagaac aaaaagcagc
     2341 tcaaaaacaa atggaatctt tccaagcaac cattgatttg aatgaagaat tagtgaagaa
     2401 gctaaaagat caattgcctt atcaagtcgc agatgatcag gaaaatactg aaaacaagat
     2461 taatagctac cagcaagaaa ttgaaacatc gaaaacgcaa ctgaaagatc aagaagagat
     2521 cattcaaaaa cttcaagaaa aaattgatcg tatgacaaaa gaaaagattt agaaggacag
     2581 gtggagtgaa aaaatgaagc gaacaaactt ggctgacaaa gatactttcg ctgagcagaa
     2641 agtattgatt aaagcctatc tcaaagttgt aaaagctaaa gaaatatgtc gagaagtgaa
     2701 tttatctaac aca
//
LOCUS       DACDHJ010000238         2683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDHJ010000238 DACDHJ010000000
VERSION     DACDHJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.242"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..683
                     /locus_tag="IV223_002429"
     CDS             <1..683
                     /locus_tag="IV223_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8712677.1"
                     /translation="KRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRAGAV
                     GIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIALDC
                     TLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEE
                     SKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIA
                     SRFVKALS"
     gene            697..2199
                     /locus_tag="IV223_002430"
     CDS             697..2199
                     /locus_tag="IV223_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8712678.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2212..>2683
                     /locus_tag="IV223_002431"
     CDS             2212..>2683
                     /locus_tag="IV223_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8712679.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKIT"
BASE COUNT          857 a          371 c          540 g          915 t
ORIGIN      
        1 aaaagaggtt tttagatctt atttcaaaag gtttgattgt ttcctgtcaa gcattacctg
       61 gtgaaccatt ttacaaagag gaagaaagtt taatgcctta ttttgctttg gctgctcaaa
      121 gggctggagc agttggtata cgtgcaaatt ctgttcgaga tattattgaa attagaaagt
      181 gtgtatcatt accaatgatt ggaattatta aaagagatta tccaccagaa tctccattca
      241 ttactgccac tatgagagaa gtagatgaat tagtagaaac cggtgttgag gtaatcgctc
      301 ttgattgcac tcttagaaat cgacatgatg gactatctat aaattctttt attactaaaa
      361 taaaagaaaa atatcctcat cagttattta tggcagatat ttctacattt gaagaaggca
      421 aaaatgcata taaagcagga atagattttg taggaactac tttaagtgga tatactgagg
      481 aaagtaaaaa acaagatggg ccagatatag atttgattaa aaaattggta gaagaaggga
      541 tttgtgtaat tgctgaggga aggattcata ctccagaaca agcaaaagag ataaaaaaaa
      601 taggagttgc aggtattgtt gtgggtggtg ctattactcg accacaggaa attgcatcaa
      661 gatttgttaa agcattaagt tagggggaga aataaaatgt ttaaaaaatt ttcacaatta
      721 ggacgcgctt tcatgttacc aattgctata ttaccagtag ccggattact attgggactg
      781 ggaggagcgt taacaaatga gagtgctata aatgcttatc catttttaga tcaaccgtgg
      841 ttgcatacaa ttttaagtat tatgagttat gctggaaatg cagtttttgc aaacctagca
      901 ctaatttttg caattggtat tgctgtagga cttgcaaatg gagataaagg aacagctggt
      961 ttagctggtg gagtagctta tctagtatat actgcaacta ttagtgggtt tcttgaactt
     1021 ttttcagcaa aagatgcaac actagataca ggtgtagtgg gatcaattgt tgttggtggt
     1081 actgttgcat ttttacacaa tcgttataga aaaattgaat taccacaatt cttaggtttt
     1141 tttggaggat caagatttat tccgattgtt tctagtttag ctgcaatcat tattggttct
     1201 ttcttttatt taatttggcc accaattcaa aatggtttag tagttgctgg tgaacaaatt
     1261 gcccaaatgg gtagtttagg aactttttta tatggttttt tattgcgttt aacaggtgct
     1321 gttggactac accacaccat ttaccctttg ttctggtata catctttagg tggtaccgaa
     1381 actgtagctg gatcaacaat tgctggagcc caaaatattt tctttgccca actagctgat
     1441 ccaaaccaca ctggtctgtt tacttatggt actagatttt ttgcaggaag atttgcaaca
     1501 atgatgtttg gattaccagc agcatgttat gcaatgtatc gtgcaattcc taagaaaaat
     1561 cgtaaaaaga atggtgggct ttattttagc ggggccctta catcattttt aactggaatt
     1621 actgaaccag ttgaatatat gtttttattt gttgctcctt ggctttatgt gattcatgca
     1681 ttcttagatg gattatcttt ttattttgct gatatcttaa atattcgtgt agggaattcc
     1741 ttttcaggtg gtttgatcga ctttttatta ttcggtgtac ttcaagggaa tgacaaaact
     1801 aattggttaa aattaattcc ttttggtatt gcatgggcat ttatatattt ctttgttttt
     1861 acattcttta tcaaaaaatt taaggtagct attcctggaa tggaagaaga atcagaagtt
     1921 cattctgaaa aagtaataaa caaaaataca tcattacatg aagagtctct tatcattatt
     1981 gaagctcttg gtggaaaaga aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt
     2041 gttgctgtta cgaatgggga gattattcaa aaacaaacaa taaaagacac tggtgcaact
     2101 gctgtcttcg aagtgaaggg tggcatccaa gctgtttttg gagggaaagc tgacttatta
     2161 agtcaagaaa tcaatcaaat attaggaaaa gataattagg aggtttttaa tatgtttgaa
     2221 attttcaaaa agaaaaaaca agaaactttt tatgcacctt gttccggaaa aatcatttcc
     2281 atcacagaag taaatgatcc tgtcttttca caaaaattac taggtgatgg ttttgccgtc
     2341 atacctgatg gttcaaaaat ttatgctcca ttggctggtc gagtaacaaa tatatttcct
     2401 actaaacatg ctattaatat aaaaagcaac tcaggcattg attatttgat tcatattgga
     2461 attgatacag ttgaattaca aggagagcct ttttcgattt gtgttcaaga aaatgatgag
     2521 gttactcctg aaacctcgtt ggttgatgtg gattttgaca aaataataaa atctggtaaa
     2581 gatacttctg ttatagttgt ttttccggag aaaaatcagt tcaatgattt aataatagat
     2641 aatggtcgaa ttgctcatgg gaattcatgt ggaaaaatca ctt
//
LOCUS       DACDHJ010000239         2665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDHJ010000239 DACDHJ010000000
VERSION     DACDHJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.243"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            171..515
                     /locus_tag="IV223_002432"
     CDS             171..515
                     /locus_tag="IV223_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8712680.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IV223_002433"
     CDS             516..935
                     /locus_tag="IV223_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8712681.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="IV223_002434"
     CDS             complement(1027..1377)
                     /locus_tag="IV223_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712682.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2563)
                     /locus_tag="IV223_002435"
     CDS             complement(1343..2563)
                     /locus_tag="IV223_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8712683.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT          993 a          481 c          521 g          670 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttc
//
LOCUS       DACDHJ010000240         2635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDHJ010000240 DACDHJ010000000
VERSION     DACDHJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.244"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..1502
                     /locus_tag="IV223_002436"
     CDS             51..1502
                     /locus_tag="IV223_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8712684.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1851..2483
                     /locus_tag="IV223_002437"
     CDS             1851..2483
                     /locus_tag="IV223_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8712685.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          990 a          378 c          516 g          751 t
ORIGIN      
        1 taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat
       61 ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca acacctttcc
      121 atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc gagacgactg
      181 aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact tcatcaagtg
      241 aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa agtagtacga
      301 cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg
      361 agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa
      421 cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat
      481 cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc
      541 aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc
      601 attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa
      661 aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat
      721 ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag
      781 gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc
      841 tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt
      901 atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa
      961 aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag
     1021 atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg
     1081 ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt
     1141 attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg
     1201 cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt
     1261 ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat
     1321 atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa
     1381 ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca
     1441 aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact
     1501 aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat
     1561 caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt
     1621 ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc
     1681 actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta
     1741 ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt
     1801 aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa
     1861 gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt
     1921 gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta tcaggtagaa
     1981 ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata
     2041 aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg
     2101 tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa
     2161 aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa
     2221 aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat
     2281 gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg
     2341 gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga
     2401 cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag
     2461 tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaga ggtactattt
     2521 aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg aaaataaatt
     2581 gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga taata
//
LOCUS       DACDHJ010000241         2627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDHJ010000241 DACDHJ010000000
VERSION     DACDHJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.245"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            596..1507
                     /locus_tag="IV223_002438"
     CDS             596..1507
                     /locus_tag="IV223_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712686.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1994..2260
                     /locus_tag="IV223_002439"
     CDS             1994..2260
                     /locus_tag="IV223_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712687.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2332..>2627
                     /locus_tag="IV223_002440"
     CDS             2332..>2627
                     /locus_tag="IV223_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712688.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDT"
BASE COUNT          942 a          457 c          572 g          656 t
ORIGIN      
        1 ccgacttcca ggtttttgac cattttctct caacgtggaa aaaccgccac ttatgagaga
       61 aaatggtcaa aaacctaagc gggaagtctc cgaataacat accaagcgcc gacagccaac
      121 agataaagaa tacactacta aaaatagtag tagcataaaa tttaaaaggg aaaaaatcaa
      181 cccctacccc agtcttgggg tctcaaaact aactcccccc taaccccctc tttcagcctc
      241 ttttgaaaga gggggagaat caaccccacg gccaacgccc cagtgttctt ggggctgtcc
      301 agaacagaaa cttgcgagag agtgggggta aaacaagtgt agaggaaact tgaagacttg
      361 ttcgaacaga aacttgcgag agagccggag taaaacaagt gtagaggaaa cttgaagact
      421 tgttcgaaca gaaacttgcg agagagttgg agtaaaacaa gtgtagagga aacttgaaga
      481 cttgttcgaa cacaaactta caagagaggg gagtaaagca gttgcagagg aatcaggaag
      541 atttgttcga acagaaactt gcaagaaagc attattaaat tgaaggtgat gaaatatgaa
      601 gtatttattg catggctatt ttattgatta ttctactcgc aatagtagta ctttagcgat
      661 tctatgtttg cttcatgaat tgagagtggt tactagaaaa cagctataca aactattgaa
      721 ttttgacaga tcaattagta aacaaggagt aacgaatgct ttagtgagat tatatgaaaa
      781 acagttaatc gatcgaaaaa aatatggaac agaaatgtat tattatttga cgaaagaggg
      841 acatcaaagc ataggtgggt attatacgtt gccgaaagtt cctgaatata atttgaatca
      901 ccatttacaa attaacaatt atttgattaa aggaatggca ttggcaaaag accacccaca
      961 tttaaaacgg attatttcag aaagacgtac cgtctatgaa acgaaggatt taaatcctaa
     1021 gacaaaagga agaaaatttt ttgtgccaga ttttattttt cgttttcgag acaaagaaaa
     1081 tcatgagatc aattggtcct ttgaaattga gctaacgttg aaaacacgtc gtcgctaccg
     1141 tgaagcgatt tttccgaaat atattcgtga gttgcgaaca cgtcctgaat cacatttaat
     1201 ttatgtgaca cctagtggcg caattcaaga agaacttcat gcgtttaagc gtattttttt
     1261 caagcgcgaa actgaaaaag atgcgagtca acaagaattg tttgagcggt tgcatatttt
     1321 ttcagcttta gagtttgaag agggattaaa agcattatta gagaatgatc cagcagtcaa
     1381 ttggcggaca gtagaacgag aaaaaattta tccgaatggc gctgttcaaa gtcgtttgaa
     1441 tttatttgaa gaatcaactg aaaatgagga aaaaccaaaa gtgaagttta cgcctgaagt
     1501 attgtaaaag agggtgttcg cttcgctctt tttttgtgcc agtttgctgg caccgttcag
     1561 acgcaagcgt ctgaaccggc tcgtttttag gaaggactaa cttgtttgat ttatccgttg
     1621 tcaaagaagt ggacgttcga gagtccctaa tcgtatagca gtcattcggc gagtataacc
     1681 taaaacataa cctgccaatt gccaagggta aaatgcacgc tgcgcgccct tggcaatcgt
     1741 cagaacatgt tttcgagccg gttatgccga acgaccgcta tacgaaaagg gcctctaaac
     1801 agaagtccac tttgatcctt gacaactgaa acccaaaatc aaacaagcag gccttcgtct
     1861 aaaaacgagc cgaatgaaga ccttgggggt tgcaccccca aacccccgtg cccccgacac
     1921 actaccgaaa aggtgggggt gggtggcgaa aaaatcaaat aaaacattct attcaagaaa
     1981 ggaagaaaca accatgacaa ctaaaaacga gcaactgaaa gaacaggcaa gaaaagagtt
     2041 gcaagaaaga ggtttgatta ttgaggggag ctttgaagga gattttgaaa cctatattgg
     2101 ttgttatgca agaccgataa acaaacctac agccttagac ccaatgaacg aacaagaagt
     2161 aaaagaacaa gaaaaacatg cagtgaatgg ttttccgcaa gatttcactg aatggtatga
     2221 gtgggaaatt aacaacggga agcttgaaaa cttcttataa aagtcagatg acgtgcaaag
     2281 agaatgattc tctttgtacg gtaaaaatat aaagaaagaa ggaaaaagaa aatggcaaaa
     2341 aacacacctt atcaagtagg agacattttt tatagtagtt ggggctacga acaaacgaac
     2401 gtgacttttt ggcaaattgt gaagttaaca gaaaaaacag catggtttca gcctattcaa
     2461 aagaaaacgg tcgagcatta ttcgtccatg agtggaaaag ttgttccaat gaaagacaag
     2521 tttatagagt atcctttagc aaaaactaaa gacagtattg taaaaaaacg aattgatcca
     2581 gaaacctatc ctgatatgtt atgtggttat cgtagttggg acacgtt
//
LOCUS       DACDHJ010000242         2626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDHJ010000242 DACDHJ010000000
VERSION     DACDHJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.246"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..2423)
                     /locus_tag="IV223_002441"
     CDS             complement(60..2423)
                     /locus_tag="IV223_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8712689.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          699 a          552 c          483 g          892 t
ORIGIN      
        1 ttaatttgac aaataataca atgtaaaaaa ttctgttttg cgggatcgat tttatcattt
       61 tattggtcaa gatttttcca tttccaatcc aatacttctg gcagatcaat accattttca
      121 cgaatatagt tttgatgttc ttgtaatttc tgatccattt tttccgcaaa atcttctgct
      181 tgacttcctt tgatttttaa agctgcgtct tttgccaaat gataacgatc aagttcattt
      241 actacacgca tgtcaaatgg tgtcgtgata tctccatttt ctttgtagcc atgaatcatt
      301 aaatgatggt tatgacgatc aaagaaaatc gtgcggacca atgtttcata accgtggaaa
      361 gcaaataaga tcggtttgtc ttttgtaaaa tattgatcaa attcttcatc gctcaaccca
      421 cgcggatctt gacttggatg acgtaatttc aataaatcta cgacattgat gaaccgaatt
      481 tttagttttg ggaattcttc cagcagcaaa gttacagctg ctaatgcttc taagttagat
      541 tccgttcctg cagcagcaat gaccaattca ggttcctcct cttttgctgt cgaagcccaa
      601 tcaatgattt tcaagccttc attcactaag acggctgctt cttctgctga gtaaaattga
      661 gcacgcggat gtttggaaga gatgatcaaa ttgatttttt ctgacgagcg gaatgcttta
      721 tccatgactg cgagcaatgt atttgcatcc gcaggcagat attcacggat gaactccgcc
      781 ttcttttctg ccaaatgtgt caatattcct ggatcttggt ggctatatcc attgtgatct
      841 tgctggaaga ctgttgagct tgcaatgaga ttcagcgaag gataatcttt tcgccaagaa
      901 aggtcatgtg atttccgtat ccatttgaag tgctgagtca acatagagtc aaccactcgc
      961 aagaatgatt cgtaacttgc aaaaaaacca tgccgtccag tcagaacgta cccttctaag
     1021 aacccttcat cttggtgttc cgataattga ctgtcgatga cgcgaccaga tggcgaaagc
     1081 cattcatcat tttctggttc gattttttcc atccattggc gatttgtcgt tttgaaaact
     1141 tgattcaatc ggtttgattt tgtttcatct ggtccaaata tacggaaatt ttctggattt
     1201 ttctcaatga tctcgcgaac gaatttacca agttcggtca tatcttctgc tttaacggag
     1261 cctggttttt caaattgcaa agtatatttt ttccaatctg gcataataag cggtcttgga
     1321 tcgattccac cattggtgat aggatttttt gccattcttt ggtcaccagt aggcgcaatt
     1381 tctctgattt cttctaaaat acgtccttgt gcatcaaata atttttctgg ttcataggat
     1441 ttcagccaac ggagtaaagc atctgcatga tccatatgtt cttgatctac aggaatcggt
     1501 acttggtgtg ctcggaaggt tccttcaatt ggttccccgt cccattcttt tggaccggtc
     1561 catccttttg gtgtgcgtac gatcaatact ggccattttg gcatcacgac atctgttgct
     1621 gtcttcaggc gagcttcttt ttggatggat ttgattttct cgattgcttg atccattttt
     1681 tcagccataa caggatttaa tttttctgga tcatttcctt caataaagaa aggctcccag
     1741 ccaagtccgt taaagtaatt tgctaattct ccatctgatt tacgtgccaa gatcgttggg
     1801 ttggctattt ttgcaccatt taaatgaagg atcggtaaaa cagctccatc tatcactgga
     1861 ttgataaaga catttgagaa ccagcttcca gctaaaggac cggtttctgc ttcaccatca
     1921 ccaatcacga cagcagagat tacttcagga ttgtctaata cagctccgac tgcatgtgaa
     1981 agagagtatc caagctctcc gccttcatgg atagaacctg gtgtttctgg tgctgcatga
     2041 gaagcaatgc ctccaggaaa tgaaaatcgc ttgaataatt tttgcattcc tgtctcatct
     2101 tccgttactt ctggatagat ctctgtgtaa cttccatcta aataagcatt cgataccatg
     2161 acttgtccgc cgtgtcctgg cccttcgata taaaacatgt tcaaatcata tttattgatg
     2221 actcggttca aatggacata aataaaattt tggccaggaa tcgttcccca atgaccgatt
     2281 ggatgttttt tgacatcttc tggtttcagt gtacgtctca gtaaggggtt atctttaaga
     2341 taaagttgcc caacagaaag ataattagca gcacgccacc aagccgtcat tttatcaaag
     2401 tattctttac tagaataatc catacataac actccttttc attattactc ttattctagc
     2461 gcagatactc ccaattgaaa cctaataagc acttctgttc atttgtttgg attttatatc
     2521 tagccaaatg aacaggtata aaaaaccacg aaatttttcc tttcgctgac gttttttaca
     2581 ttgatccaaa aaacttgtcg ttatcacaat ggctgttccg cagttc
//
LOCUS       DACDHJ010000243         2606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDHJ010000243 DACDHJ010000000
VERSION     DACDHJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.247"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            385..1824
                     /locus_tag="IV223_002442"
     CDS             385..1824
                     /locus_tag="IV223_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8712690.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2119..2337
                     /locus_tag="IV223_002443"
                     /pseudo
     CDS             2119..2337
                     /locus_tag="IV223_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          734 a          468 c          580 g          824 t
ORIGIN      
        1 tttagtataa tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa
       61 acaaataggt gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaatttgc
      121 ttacgtctca cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga
      181 aattgctaga agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa
      241 gcatctccaa cttacatggt tacatgagga tatcaaatcg aaattactca agcttcctat
      301 ggctagagtc gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac
      361 gatcattaga aaggaaatcc tattatgtat agttatcaag aagatgcaaa agtccaaaaa
      421 aatcgctggt ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc
      481 agtattgtca atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa
      541 gcggaatggg tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt
      601 aaaattggcg atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc
      661 ttgggatcgc tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt cgcacgggtg
      721 gtacaagcca ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc
      781 tttcccatac atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt ttcattaggc
      841 tcgattgctg gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt
      901 ttttggatca atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca
      961 aaagatatca ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct
     1021 acgattatca gcttttttgg agcaatcttt ttggggcaag aaagtggctt tttccatcca
     1081 gtcccccttg tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa
     1141 aagaaagcag cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg
     1201 agcttgatta cagccttgtt gattttttct tccaacttct ttgtcaatgt agtgattcct
     1261 ttttatctgc aaaatgctcg cgggttgagt cctagctatg ctgggctgtt gatgatggtc
     1321 tttcctttga tgatggtcgt cggggcgcct ttaagcggct acctgaccga ccgggttggc
     1381 ccggagattt tggtattagt aggcttgggc ttattgtcgg taacccaggt catgtatatg
     1441 tttttgtcag tggactctcc tttgtggcta tacattgtcg ctactgccat catgggtgcc
     1501 ggcaatgcct tgttccagtc ccccaataat acgatggtta tgagctcggt cactatcgaa
     1561 aacttgggac tggcaggctc gatgaattcc tttgcccgaa atttggggat ggtcagcggg
     1621 atttcgcttt ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccagcaagta
     1681 actaactata tcgcaaatcg gccggatgtc tttttatatg gaatgaagat taccttttta
     1741 ggcagctttg ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa
     1801 ggaggtattc agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg aggcctgcgt
     1861 atgggtgagt gggatttcca tggatgatgg aagatgaagt gaacttctaa aagccgaaaa
     1921 actcaagtat tatcttcaaa atactagtgc tcaatgagat gcttgatttg atcacaagaa
     1981 gactgcgaaa gccgctggca accgcaagct aggtatccca ccgatggact acgttttaga
     2041 ggctactggt tatgaatacg gagccaacgc tctcatctgc ataagaatac atcacccaga
     2101 ttatgtaaag attttcgata cttctgtcaa aaaatcatta agatagataa atgatttccg
     2161 tctagtcaaa ttggttcaga atgtagacac acagatggta agaagtcact cacaattcga
     2221 gaatggactt gtcctatttg ccgtatacat cataatcgaa acgtcaacga tagtatcaat
     2281 attttgacca agggctatga atacaagcat tgccttagaa aacatcaaga accttaggaa
     2341 caacggggat agcttggtta ataagagata ccgctgttta ggcttgcaag tatactcttt
     2401 tatcaagaat tccgtggctt tagtcatgag aggcttaata ctatgaaaat tttctttagc
     2461 atttttgatt taggttcctt ttctaattat aataagatga ccactacagt aacctactga
     2521 aatatataaa cagccactgt agtaccattt tcaaagtagt ctatgagagg agaatcttca
     2581 ttcttatgaa cgctggaatt gcggat
//
LOCUS       DACDHJ010000244         2549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDHJ010000244 DACDHJ010000000
VERSION     DACDHJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.248"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..511
                     /locus_tag="IV223_002444"
     CDS             89..511
                     /locus_tag="IV223_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8712691.1"
                     /translation="MWKEKKTYQEYLNKLVAPLIRGYYVDEEFEKRILKREYQQSTFV
                     NKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFLFAIPEEMTAEVENELFEL
                     YDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            504..995
                     /locus_tag="IV223_002445"
     CDS             504..995
                     /locus_tag="IV223_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8712692.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            983..1537
                     /gene="srlA"
                     /locus_tag="IV223_002446"
     CDS             983..1537
                     /gene="srlA"
                     /locus_tag="IV223_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8712693.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            1555..>2549
                     /locus_tag="IV223_002447"
     CDS             1555..>2549
                     /locus_tag="IV223_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8712694.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT          847 a          417 c          554 g          731 t
ORIGIN      
        1 ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg
       61 gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt atcaagaata
      121 tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag aatttgaaaa
      181 aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat ttgcttttcc
      241 tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa taactgatca
      301 aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac ctgaagaaat
      361 gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg aactcataaa
      421 aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt taaaactaat
      481 caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt ttcgcagctt
      541 cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc aatcaaacat
      601 atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg aaaatccgtt
      661 caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa gtcagaaaaa
      721 tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg ggtgaagata
      781 tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg acacaagcag
      841 ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa aaaagtgaac
      901 aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa agtacgatca
      961 aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg gatttatgag
     1021 gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg taccagttgt
     1081 cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag accgagtaac
     1141 gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt taccgttctt
     1201 atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc ttccagaata
     1261 ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca atggtgtatt
     1321 cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag ggatcgaaac
     1381 cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac tgctgatgaa
     1441 ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac agcaaggaat
     1501 cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt ttaaatgtat
     1561 ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat tattaggccg
     1621 acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc aatgatcgta
     1681 gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac atcaatacct
     1741 gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg tggaatttat
     1801 cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg cccattagca
     1861 caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat acagccacta
     1921 atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa aaagtacaaa
     1981 tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa
     2041 attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag agaagcagta
     2101 gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt gattggtgtg
     2161 atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt agcaggaaat
     2221 gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc tcctttgctt
     2281 ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga gattgggaaa
     2341 ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac tcaaaatgca
     2401 tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac agtagaagta
     2461 ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt tatcattgct
     2521 tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACDHJ010000245         2542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDHJ010000245 DACDHJ010000000
VERSION     DACDHJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.249"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1313)
                     /locus_tag="IV223_002448"
     CDS             complement(<1..1313)
                     /locus_tag="IV223_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8712695.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIP"
     gene            complement(1332..2132)
                     /locus_tag="IV223_002449"
     CDS             complement(1332..2132)
                     /locus_tag="IV223_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8712696.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2356..>2542
                     /locus_tag="IV223_002450"
     CDS             2356..>2542
                     /locus_tag="IV223_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8712697.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPM"
BASE COUNT          717 a          498 c          393 g          934 t
ORIGIN      
        1 aacggaatcg ttgtaaaaac agcgaaatat ttgtttagat ctactttttc atattgttct
       61 tctgaaacat gtgttatttg tatatccctc cccaatatcc ttttgatttg tctttggatc
      121 attaatgctg ttccttttcc tgtcgtatga acaatggcta cttttttaga ggaaaaatgc
      181 tctttttgat atcgaaatgc taactccaaa tacaacgtta ggtagctgat ctctacttta
      241 gagatcttat gttgcaagat agcttctaat gcttgtatgc attcgctagc gatttggtaa
      301 gaaaaaggat actttttttc tatctcacca taaaataaat cagtcaatcg ataacgaaaa
      361 attacccgat ttatcaataa agagaggtgc tgtttcatct cttgatataa gaaggactca
      421 tccagatcaa tgagatctaa tcgtcgaata cgcatcatca tggggtcgaa atataagcgc
      481 aaatcagagt tagctttcct tatcatcgtt atacttgtgt tgatgatgtt caaaggaaaa
      541 ctgataaaat ctttttcata ttggctcaaa gttagttcat agatttcttc caactgattg
      601 atcagttcat tgaattcttg gatatattca aaacacccac tgtaacgatt gatgggcttc
      661 ttcaacagat agttactaga gattctattc aaagaaatac cgataacttt cgttaaaagg
      721 gtgatttctt gtttactagc attcgtcttc ttctgaaaat gtttgattgc ttgtttttct
      781 tctagaaact gttctgtttt cgtctctgaa aagtagggag aaacaaaata aaataaaagt
      841 agtcgtaatt ctagctcatc tcctattatt cttagtcctt tatttggtgt gcctgtaatc
      901 tttacatcaa aatcattgat catctctttt aatgtcttta gatccttgac cactgttcct
      961 ctgctgacac cgacttcttc agacaaatca tccatacgta agaaatcctc tgtttcgatc
     1021 agcctattta gtaaataagc gattcttttg gatgccgaat tgaaatcact ggctgttttc
     1081 aattttcctt gcatgatctc atcaaaaaaa tcaaaatcat aaatttcaag ttcaaaatgt
     1141 ttgttttttt ggatgatttt tgcaataccg gcaatttctt cattcaactc cccaatatct
     1201 ttttttatgg tttgattact ggttttgatt tctttttgca cttcctggat tgaaactttc
     1261 ttgtgagtga ctagtaattt caataattgg taccatctgt ttactagcgt cacgtgcgtc
     1321 ccccctatcc tttatcctct tgttttacct cctgaaatat tagtcgtgac tccagtgata
     1381 taacttgatc tttctgaaag ataatagcag actagatccg ccacttcttg cagcttacca
     1441 ctccttccta gagggatcgt acttgttcga ctgtaaccgt ttctcagctg ttcgacgctt
     1501 atacctctcg tataagaaag agcttcttca tattcttgtg ttctcaaacc ggtttcttcc
     1561 agtatccctg gtgcaacacc taccacacga acattcctct ttcctaactc ttttgcccaa
     1621 gaacgagtaa aactgttcat cgctgctttt gtagcagcat aacagctctg tccttctgat
     1681 ccttccaatc ctgattcaga agaaagattc acgatcactc ctgatccctt ttgcaccaag
     1741 attcttccaa ctgcttggct catcaaatat acactttttt gattgacagc gatcatctta
     1801 tcaaaaactt gttctgataa ttcatacttg ctttttggat gatttctatc tattagaaga
     1861 gaaggaatat ttattcctgc attattgact agaccatcga tcgtcttgaa atgtttcatt
     1921 actttatata tcccttcttc tatgtccgtt ttagaagaca catcgacttt taaagatagt
     1981 aggttttcat gctgtatacg gcattcagtt acatcaaaat tcgctacttg tgcattctgc
     2041 ttcaacaaat tttcgacgat acttcttcca attcctgagg aacctccagt tacaattact
     2101 acttttcctg caatatccaa ccaatccgac atatgtgatc ctctccttct tttttcttac
     2161 aaatccagta taaaagctca ctaaaaaaaa ttaagacatg aaatttacag cttcagctaa
     2221 aaaatgtgta ctaatggaag agcaaaaaaa attttcatct ttttgcatag attgctaaaa
     2281 ttcctgagcg ttatataagc aatgtttctc tttttgatat ataattgttt tatcatttaa
     2341 gaaaggagaa ttctaatgaa aactttagat gctattgcat taactttatt gattgtggga
     2401 gggttgaact ggctgttggt aggcttgttc gaacttgatt tagtagctat gattgctggt
     2461 ggttcaacaa ctatttttgc gaagatcatt tatatcgtcg taggtatttg tgcgatctac
     2521 tgcttgaaat ttttcccaat ga
//
LOCUS       DACDHJ010000246         2533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDHJ010000246 DACDHJ010000000
VERSION     DACDHJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.250"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..692
                     /locus_tag="IV223_002451"
     CDS             66..692
                     /locus_tag="IV223_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8712698.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            715..1419
                     /locus_tag="IV223_002452"
     CDS             715..1419
                     /locus_tag="IV223_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8712699.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            1611..2444
                     /locus_tag="IV223_002453"
     CDS             1611..2444
                     /locus_tag="IV223_002453"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8712700.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT          754 a          447 c          519 g          813 t
ORIGIN      
        1 ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag ttttggctat caaggaggaa
       61 tgcacatgca tatcgaaaga ttaaaaacag gaatgattga agaaaactgt tatttggtct
      121 acaacgaaga agcacttttg atcattgacc ctggagaaga tgcggaacga atcaaacaag
      181 aaatcaaaaa aacaaaccaa cagccggtag cgattttgct tacccatact cactacgatc
      241 atatcggtgc agtggaacct ttacgcgcgt attatcaaat cccagtttat gtcagcccac
      301 tagagcaaaa atggctaggt gatccgattt tgaatctttc tggactcggt agacatgatg
      361 atattgctga tgttattgtc tcgcctgcag aatatgaatt tgaaatgaaa acttatactt
      421 taggaaacat gtcttttcgc gtcgtcccta ccccagggca ttctatagga agcgtcagct
      481 ttatttttga tgattttgtc gtttctggcg acgccctgtt tagaggaagt atcggtagaa
      541 ccgatctgta tacagggaat ttagaacaat tacttcatag tattcggacc cagctgtttg
      601 tattacctga tgaatttgca gtctatccag gacatggcga tgcaacgacg attgaacagg
      661 aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt aggaggagaa taaaatgaca
      721 gaaactacac tttatatcgt acgacacgga aaaacgatgt tcaatacaat tgaacgagta
      781 caaggctggt gtgatacacc cctcacaaag caaggacaag aaggcattca ttatttaggt
      841 cgcgggttaa aaaatgtcga cttttcattt gcttattcta gtgacagtgg tcgtgcaatc
      901 gaaactgccc gtattattct aggagaacat tctaagggaa aagaaattcc ttattttatt
      961 gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag gtggatatga catggaaatg
     1021 tggggtgtga tccctcgtgt attgaatttt aaaacatatg atgaaatgtt taccacagaa
     1081 gtttcttttg aacaaattgc taatgctgta tacaacttag ataccgctgg gtgggcggaa
     1141 acttatgaaa cattgcgaga ccgtgtatgg acaggatttg aagatatcgc ccatcactgc
     1201 gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg gtttgacgat cgcctttttg
     1261 cttaatctga tcgatcctaa acagcctgtt cgtgctgggc ttttaaatgg cagcgtaaca
     1321 aaggtcactt atgcaaacgg tcgattttct atccacggca tcaatgatac gtcttatatc
     1381 gataacggga aaacacatag tcgcgactcg tttctataat ggggatattt taatttttac
     1441 cagagaatca taaaaggcaa agtctgaacg agggctttgt cttctttttt ctcttcgtgt
     1501 acttatctgc tttatttctc aagtttttct taatattaaa aaacagattg ggtttctcaa
     1561 aaaataacgg tatactataa gatgtatctt aattgggaag gaaggaattt atgttttttg
     1621 caaatattat caaagcagtg attctcggta ttattgaagg aataacggaa tggctgccca
     1681 tcagtagtac tggtcatttg atcctagcag atgaattcat caaactggat gccagttcac
     1741 aatttatgtc gatgttcaat gtcgttatcc aattaggggc gatcttggct gtcgtcgttc
     1801 tttattttca caagttaaac ccattttctc caagtaaatc aacggtagaa aaaaaagata
     1861 cgtggacact ttggtttaaa gtgatcgttg cttgcatacc agccatcatc attggtttgc
     1921 ctttggatga ttggttagaa gcaaatttcc acaagttctt accagttgcc attgttttga
     1981 tcgtttacgg tattgccttt attattgtcg aaaaacgcaa caaaaacaaa acacctcgtt
     2041 ggacttctct gaatgacttt acgtaccaag cagctcttct tgttgggtta ttccaagtct
     2101 tggcacttgt tcccggaaca tcccgttctg gcgcaacgat cctaggatct atcttgattg
     2161 gtgcttcgcg ttttgtcgct acagagtttt catttttctt agggattcct gtcatgtttg
     2221 gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg gaattcattc aaaatggaag
     2281 aaactgttgt tcttcttgta ggaacattcg tatcttttgt ggtttccgtc ttagctatca
     2341 aattcttact aagctatttg aagaaaaacg actttaccgc ttttggctgg tatcgtatta
     2401 tccttggtat cattttgatt ggttattggt ttattgcaat gtagaaaaaa ccagaacact
     2461 tctcttattt gagaaatgtt ctggtttttt attttcttta ataataatca ttataaataa
     2521 taaaaacaac atc
//
LOCUS       DACDHJ010000247         2526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDHJ010000247 DACDHJ010000000
VERSION     DACDHJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.251"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            421..801
                     /locus_tag="IV223_002454"
     CDS             421..801
                     /locus_tag="IV223_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8712701.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            783..1697
                     /locus_tag="IV223_002455"
     CDS             783..1697
                     /locus_tag="IV223_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8712702.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            1657..1935
                     /locus_tag="IV223_002456"
     CDS             1657..1935
                     /locus_tag="IV223_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712703.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            1942..2484
                     /locus_tag="IV223_002457"
     CDS             1942..2484
                     /locus_tag="IV223_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712704.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFNRIRQ
                     KSPILYRWVMNAVRYEGYRW"
BASE COUNT          926 a          474 c          507 g          619 t
ORIGIN      
        1 agaatcaatt ctgattcatc agtcccataa attatagagc cgaaaaatta tcatatttat
       61 agatttatta atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat gtagctctac
      121 ctaattattg atttgcaaca atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
      181 ggctagcatt tgctagtctt ttttgtattg tggagagtgg ggggggaggg ggattcaagg
      241 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
      301 caaatataga gcttgccaag ccctttagta agctttagtg agctttactt attgtacctt
      361 tttaggtaca atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt
      421 atgagcgaac aaaatcaata tttggctagc aatcattcta aaaaatatac ctatcgatcc
      481 gagccaaaac aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcagtca
      541 gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt
      601 ctggtgacac cgaaagtggc gccagacatt gctcaggcaa tcgcgcgcga tttagccaaa
      661 gctggcggga atatcaatca aattgccaag tggtgcaaca cacaccaaca cgacgttgat
      721 cctggcgacg cccaacgctt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag
      781 atatggcaac agttaaagta agccgaacca cttcttgtag ccgagcgatc aattacgccg
      841 aaccacgagc aaccgtcaaa acagggataa actgtgacat tgattatgcc aaaagcgaaa
      901 tgaaacaaat tcgcatgctg tatggaaaag acgatcgtgt acaagcacac ttattgatcc
      961 agtcttttcg accaggagaa attaccgctg aaaaagccaa tcaactaggg gcagagtatg
     1021 cgaaaaaaat agcccctgac caccaaatcg cgatttacac acatacagac aaagaccata
     1081 ttcataacca cattgtcatt aactccgtca atctggaaac tgggaacaaa tttcaagccc
     1141 atggtcaagc atttttaaac aaatgttatg acctaaatga cgagatttgt cttgaacatg
     1201 acttaacaat cactgaacga gggaagaaac ccgaaaaacg aaccatgtct gaaatcaaat
     1261 taaaagaaaa aaatgaacct gtttggaaag acgaaattcg gtttgcgatt gatcaaacaa
     1321 tgaaaaacaa aaaaactcga acgtataacc aattttgtga ttctcttaaa acattcggca
     1381 ttcattgctt gaatcgtggg aaaaatttta cttacgaatt gatccacaaa aagaaaaaag
     1441 tacgatccaa caaactagga aaggattacg aaaaggagac gattttacat gagctggaca
     1501 gacgagaacg agcctacaat caacaacgaa ccaacgagct acgaagagct cgagctagca
     1561 ttcgcgaaac tgcaaaagaa gtacgaagag aacaacgaac tcttacgact gaacaaccgg
     1621 aaattagacc gactcgaaga aaccatgacc aacacgttga aagaggagca gactaccaac
     1681 aagaattatc gttctaaaac gttacaagac atagaaaatg aaataaagaa acaacgtgct
     1741 tttcttgcac cttataccaa agaaagaata caaactacaa ttgattcaca agtcaaaaaa
     1801 ttccaccatg cgctattaaa ccagcgaaat aaagaacaga aaaaagtaat tatgcagcga
     1861 tcaatggata ttcttgcccc cttatgttgc ttaatcatga cactgttagc cttttggctg
     1921 gtatggggag aataaataga aatggcattc tatttaccct atctactcat ttttgccagt
     1981 attttaggaa ttatttggct tagctataag gcttatcaaa caagatattc attaaaaggc
     2041 tctaaaattc gatttaagag gtttttattg ccaggggtta tatttacatt atcaatcgtt
     2101 ttaagtggtt tattcggcgt tttagaggga aataaacgta tttcaagaag tattttatta
     2161 ggggatatca ctcaaaagta tgaagcagca tgtgataaaa agaaaaagga gcaagtgtta
     2221 acacaaaaaa tggaagagtt taccacttgt tatgaggaaa tgaacgatat ttttgtgaat
     2281 caagaaaaac gtttgacgga taaaaacatg gaaaaactca ctcgtcttta tcaaaacttg
     2341 ccagaaaaac agcaaaaaga ggttcaagac aactatgagc gaaccaaaaa agacgtgcaa
     2401 tattttaaca gaattaggca gaagtcgccc atcctctata ggtgggtgat gaatgccgtt
     2461 cggtatgagg gttaccgttg gtaactcgaa gaccgacaac taaggcgtat tgccttttta
     2521 ttttgt
//
LOCUS       DACDHJ010000248         2523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDHJ010000248 DACDHJ010000000
VERSION     DACDHJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.252"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..2453
                     /locus_tag="IV223_002458"
     CDS             12..2453
                     /locus_tag="IV223_002458"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8712705.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          837 a          455 c          598 g          633 t
ORIGIN      
        1 aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt
       61 atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat
      121 tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt ggaaatgtcc
      181 tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg
      241 tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct
      301 tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga
      361 gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac
      421 caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat
      481 taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag gatcgctatg
      541 ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga
      601 tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa
      661 atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa
      721 cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag
      781 aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg
      841 gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata
      901 gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt
      961 cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag
     1021 gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag
     1081 cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag
     1141 gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa
     1201 aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg
     1261 tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg
     1321 atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc gcgcagaatt
     1381 ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg
     1441 cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc
     1501 tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag
     1561 ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga
     1621 aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat
     1681 acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact
     1741 cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat
     1801 tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat
     1861 tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg
     1921 caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga
     1981 atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga
     2041 tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag
     2101 gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt
     2161 ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg
     2221 aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat
     2281 tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct
     2341 caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa
     2401 agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca
     2461 atatctttag aagaataaag caagatttac agttatttta tttactgcag atcttgcttt
     2521 ttt
//
LOCUS       DACDHJ010000249         2521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDHJ010000249 DACDHJ010000000
VERSION     DACDHJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.253"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..2376
                     /locus_tag="IV223_002459"
     CDS             4..2376
                     /locus_tag="IV223_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712706.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     VPTDFLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VREIEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1009 a          395 c          555 g          562 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta
      661 ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gttggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 g
//
LOCUS       DACDHJ010000250         2503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDHJ010000250 DACDHJ010000000
VERSION     DACDHJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.254"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            219..2384
                     /gene="acm"
                     /locus_tag="IV223_002460"
     CDS             219..2384
                     /gene="acm"
                     /locus_tag="IV223_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8712707.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          992 a          366 c          474 g          671 t
ORIGIN      
        1 gtaaaataaa atgaatatat gcgaaaaaat aatttacatt aggaaaaacc ttgtaaaaac
       61 agttttttgg catatttatt ttttataaat atttgtaaaa taactatcaa ttttttagtt
      121 aaaagattgg aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt
      181 aggttttatt tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta
      241 ttttatcaat gttatttatt attactaata taacatcact gattcctgtt catgtctatg
      301 cggatgcagg cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata
      361 ttacagatgg aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc
      421 aaccaggtga ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat
      481 tccaaaaaga aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa
      541 aagattctgc gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt
      601 gggggcaatt tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac
      661 cattcaccgt gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg
      721 gttcttcttc tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc
      781 acattcgatg gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa
      841 tcagtgatga aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa
      901 tacacttagg tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag
      961 atttccccgg cgctacattt aatttttcag taactgataa ttacattgaa ataactatac
     1021 caaaaaactt tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc
     1081 cagaacaaat aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg
     1141 ctgttgatgg agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca
     1201 acggtacagt tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta
     1261 tcccaaatgt gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta
     1321 gtatcctgtt gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag
     1381 actatgttgt aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa
     1441 atgaactcaa attctccata aacgaaaatg atacagaggg agtcagttta cctatttata
     1501 acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa
     1561 gaccgagtat ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg
     1621 atgcagaaac aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac
     1681 agtatgatga tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa
     1741 atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga
     1801 ataaagaagt catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg
     1861 gcaaacgacc gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca
     1921 aagaagtaag tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag
     1981 acgggcaaga aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga
     2041 ttgacgggta taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag
     2101 gtagccatat aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa
     2161 atctattctc tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa
     2221 gcaattatat tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc
     2281 ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa
     2341 ctatctgtgc tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt
     2401 tttgttaaat atgactgaaa tcaaaagaat aaaattccga tttataatcg aaaaaagaag
     2461 catttttata tatgcttctt ttttcgtaga atcaataaat atg
//
LOCUS       DACDHJ010000251         2431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDHJ010000251 DACDHJ010000000
VERSION     DACDHJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.255"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(872..1390)
                     /locus_tag="IV223_002461"
     CDS             complement(872..1390)
                     /locus_tag="IV223_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8712708.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          790 a          447 c          352 g          842 t
ORIGIN      
        1 ttattaaata cacctacaac ttataaattg atggtattta gataacacga aagaataaaa
       61 ataacagctg atagtaaagt ttgttgacga ttgctactca atcgaaagtg agttagtgcg
      121 tatgaacttg cttctgaaaa cttatgaaat gaaagacctt ttacatcttg cagaaatcgt
      181 tactgggatt aaactaagca ataaaaacta atgttcccct cattctttga acttctacta
      241 tttgatttat ttattctgat aaatgacgat ttcaccttca ggatcttcaa atcccgtata
      301 gaatttattt aagatctctt cagtatcagt aataggatag gcgataggcc acataccctt
      361 tgatggttag aataaactgc tcaaaatctg tatcgtatgt tggctttagc atattaacaa
      421 aggctatctc caaattataa accataagcg ttgaatcgtt cttaacaatt tcaattcttc
      481 ctaacccaaa gtcagaagat ataattaccg gacttgcctt acccttcatg tgggttgttg
      541 agattccata tttaaaggga attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa
      601 taaaaaaatc tttcccctgc tagtctccta agtccctttg tagcggatat ggcaccataa
      661 tctcatttta tcgaagtgag tcatggttta tctgttggcc aaccaaaatt gatacaatct
      721 aacgatgcac cctctacggc attctagggg agtgcacttg cattcaccca ataaaaaccg
      781 gcagttagca aagaacttat gtagtcgtat gttcttactg ttaacataca agaggtctcc
      841 tttccacaat tgcttagcag taaacgggaa tttacaaagc taatctcttc cattttctta
      901 tctttgttct aaaagtccca aatggagcaa ctgtatttac atgaataaat ttatatactt
      961 cccatacagc cgttttggtt gcctcgtctg cccattttct tttgtgtgca gtgaatagat
     1021 cttcatcact cattgaatca atcatcatgt taatatcatc aatattacga ttgagaattt
     1081 cctctaattc tttcaatggt agatgtgcat aggtatttgt aaaccactta tataactctc
     1141 ctaactgatt ccatttaaat tgatcagaag gcgttttaac agataatcca tttctttcat
     1201 cattttccca ttttagaatt aaattcgtcc aaccaacttg atatgctaaa ttttcagcag
     1261 gtgttctatc cacttcctca actcttttat ccttcaattc ttcaggaata gtttcaaatt
     1321 ctaaaatata tttagtataa gtcctattta tttctttttt taattcttct ttatttttat
     1381 atgttctcat tatattacct ccataaattc ctaattgtct cctttaattc atctataatt
     1441 tcatccacga tattttaatc cgttctacaa tgcatatctg atatgcattg actgtctaac
     1501 tcatatgtga atgatttata aaatatccca acgtctcatc taaagaaatg ttgatcgtca
     1561 gcaaggagct aaaaaatacg aatatatttc ttttgtagta aaatcggact ggtttgcata
     1621 tcattttctt cgtaaatatc aaaatcatgt taactactat caattactaa taaaaactac
     1681 cactttaagc gttaagaact gttaagttta gttaagtgag gatagttcac aaaatccatt
     1741 gttaaacttc acgaaagtgg taaaactcaa aatgctttat ataggattac agcatcgctc
     1801 tttcttcgat tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg atgacacgtt
     1861 ccttacggct catcagatga aacagctaca aaaacgaaat gatcaactcg aagaaaattt
     1921 aattctaaaa aagcgattgc catattcacg cctcgctcga cgaactatta aaggtcaaga
     1981 tttacctgaa gatcatcctt tttaggaaat tgaagctttt tatgttgcac aatgcattta
     2041 taatactaca atgatgtttt caccagatat tcttctaggg tgcggtgtaa tgaaacagat
     2101 acacttgcaa aagaaaattc accgcgaatt tactaaaatt atgaataatt atgttaatct
     2161 tccgaaattt ttttccaaaa ttaagggatc aagctggtgt tatagattgt ttaaggttgg
     2221 caaaaaaata acttcttttt aaacaattat agaaaaccga tactctgtta agttggttga
     2281 cacggtgtcg atttttatga ctttcttatt ctgaaacttc actaacggac agtacctaaa
     2341 aagtctatag tatggacgtt tacgagtact attttatcaa aaatgctgct catttcaact
     2401 tatatgtttt agtgaactac ccccacttag c
//
LOCUS       DACDHJ010000252         2417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDHJ010000252 DACDHJ010000000
VERSION     DACDHJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.256"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..469
                     /locus_tag="IV223_002462"
     CDS             <1..469
                     /locus_tag="IV223_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8712709.1"
                     /translation="KKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETKSLFLK
                     DKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQI
                     PIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            506..1516
                     /locus_tag="IV223_002463"
     CDS             506..1516
                     /locus_tag="IV223_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8712710.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1631..2344)
                     /locus_tag="IV223_002464"
     CDS             complement(1631..2344)
                     /locus_tag="IV223_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712711.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          815 a          400 c          493 g          709 t
ORIGIN      
        1 aaagaaattc ttacacgaaa atatatattt tgagttgtat catcatcgag ccatttatac
       61 aaatgaagat gctttggtaa taaagaaaga acaaggattt acaggtacag agaccaaaag
      121 cttattctta aaagataaaa aagataataa ctacgtatat ctgactttta ctacgaaaca
      181 aacggatttc aaagcattag ggaagatcgt cggtaaaagg ctttctattg tttcatcaga
      241 acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt tctccgtttg gttatgaaaa
      301 acagattccg attattattg atgaagagct cttatcaaat gaaaaacttg tttttgcacc
      361 aggtagacca gatcaaacga tggtcatcca aacggttgac ttaaacaaaa tcatccagct
      421 acttgagatg ccgatatttt tgatgccggc aaacgaaaaa aacaattaac caagtaaaat
      481 agtgcatact tggaggtgaa gcaacatgat aagttatcca ttattagaaa gaaatgcaac
      541 gattggggtt actgcaccat cctcaggcgt atcagaggaa caccattcgt tactcgaatc
      601 agctttaaag agaatggaag aaaaatcttt ccaagtagtt actggaaaga cgctttggac
      661 gcaagataag gaaaaatcag gtccagcaag aaaacgagca gatgaattca atcaaatgat
      721 gcaagatgaa aatatccaaa tgatctttcc gccttgggga ggagaattat tgattgagat
      781 tctcgatttt gtcgattttg atgctctaca accaaaatgg atagtaggtt attcagatat
      841 tagtacgttg ctacttgctg tcacattaaa aacagggata gccactgcac atggaaccaa
      901 ccttattgat ttaagaggag agtacttgga tgaaacgaca gcaatgtggg aaagtgtact
      961 gaaaacaaga gaaggagaat cgattctaca gcaaccttct gaaaagtatc aaaaagagtg
     1021 gccccatgag cattcatcat cttctgtttt ccatctcact gaaccaacaa attggcaatc
     1081 agtcagcgga gatgcagtaa ggatgaaggg acgattatta ggtggctgta ttgatacgat
     1141 cagtcattta atcggtaccc cttatggaga tgtccaatct tttaaaagaa agtataatga
     1201 tggaaagccg atcttgtggt ttctagaaaa ttgtgagcta tcgtcagttg atttaagaag
     1261 atcacttgtt cagatgaaac tggcaggatg gtttgatggc agctcgggga tcatgttcgg
     1321 tagaagtgca gcagaggatt tgttagatta taaaatgaag gatgtatatg aagatctgtc
     1381 aaaggaatta aaactaccga tcttgtatga tattgattgt ggtcatgtac cgccacaact
     1441 gacattgatc aatggtgcct atgcagaagt agcggtagaa gatagaaaag cgactgtttt
     1501 gcaaacgttt atttaaaaaa ttttttcctg ccttggagtt tatcaaagac caacaaatct
     1561 aaatagaaaa gaaaatgaaa gaagctgaca gtattttgcc agcttctttt tgtatgatct
     1621 attagaaatt ttaattatcc ataactatca cattgatcag ctgtttcaca tcatttggat
     1681 tacctgtagg aatagctact acagatgcat tatcaataat ttcttgagtt gtttctctaa
     1741 tgaacgctgg ataagatgtt tgatctgcag gcatttgcca atgtgaagga actggataac
     1801 tagtgattgt cccgtcatgg ttacttgaat acactaaaac tccctggtaa ctgtatttcc
     1861 cagtgagcat aaccacatct ttcggataga ctgcactccc tgcatcataa ggggcgatag
     1921 gtgttcctgc aggataatga ataacatgta gttcgaaatc acccgatgta tctctaaaag
     1981 tattacctct aattgctagc caaacacgtg cgtactcaat ttgtagatca gaatatcctg
     2041 ctaacggatc tattgactga gaagactgtg aggaagttgt gtctattgtt gaagaagaaa
     2101 tatttgtctc aactgtacta ctagatgtag aagaagtatt gggctctgag actttactgc
     2161 ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg agtacttgat ttttcagctg
     2221 aactggtttg tttgattgaa cttttgtctg tggtcgcaga tggatcacta ttttctttac
     2281 taggagacgt acatcctaca aggaataaag agaccaacaa taaaataaaa cctaactttt
     2341 tcatataaaa tacctcatta tttttttaat accagtatag cataatcctg aatttttgaa
     2401 tccgtaaaac cttgtct
//
LOCUS       DACDHJ010000253         2375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDHJ010000253 DACDHJ010000000
VERSION     DACDHJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.257"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..940
                     /gene="yidC"
                     /locus_tag="IV223_002465"
     CDS             11..940
                     /gene="yidC"
                     /locus_tag="IV223_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8712712.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            1043..2375
                     /locus_tag="IV223_002466"
                     /pseudo
     CDS             1043..2375
                     /locus_tag="IV223_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          822 a          434 c          461 g          658 t
ORIGIN      
        1 gaagtattct atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct
       61 gctcttttta tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcaggaat
      121 cgtttatcga gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgagaattt
      181 caattggtct tacggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt
      241 gccactaggt atcagtcaat caaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt
      301 aaaaccacaa gttgaagcag cacaacaaaa actaaaagct gcaacaacac gtgaagaaca
      361 aatggcagca caagcagaaa tgcaacaagt atatcgcgaa aacggattga gtatgaccgg
      421 cggaatcggc tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtattttac
      481 ggctcgctac acagaaggta tccgtgaatc atctttttac ggcatcgatc taggctcacc
      541 aagtatggtg cttgttgcca ttgcaggggt cgcttatctg cttcaaggat atatttcaac
      601 aatcggtatt cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc
      661 tatgatcgtt ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtggg
      721 tggtgtgttc agttgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa
      781 agcacaagtg gcagaagagt tgaaacaaaa tccgccaaaa caagtcgtca ctccaagaaa
      841 agacgtgaca gaatcggcaa agccagctga caataaacaa ccaaaaaaca gtacaaacaa
      901 caaaggccgc aacgcaggaa agcagcaaaa gcggaaataa aaatatatga caaaaaactg
      961 tagacaaata aaaaaatttc tttgttctac agtttttttg ttacaatcaa agaaaacgtc
     1021 gcttttcgag gaggaatgta taatgatgtc caaataatca atggatagtc gaatggaaga
     1081 agccaaaatt tcgctagatg cacttgttcc taaaaatcat ttggtccata aaattgacaa
     1141 aatattgtta tttgatttta tttatccaat tgttgaatca acttactcga ccattggtcg
     1201 tcctagcgtt gatcctgtca tcttagtaaa attagtattc attcaatatt tatttaggat
     1261 tcgttcgatg cgtcagtcca ttaaagaagc tgaaacaaac ctagcttatc gttggtttct
     1321 tgggcttagt ttaatcgata aaattcctca tttttcaacg tttggtaaaa actatgtacg
     1381 gcatttcaga gaaaccgatt gtttgaacag atatttatca ttatccttga acaagcagtt
     1441 gaagctagat ttattcatga agaaattttg tatatggatt caacctatat taaagcgaat
     1501 gcgaaaaaac atatttttac aagagaaatg gtacataaat aagccatttt ataccaaaaa
     1561 caattgaaag aaaaaattaa tgaagcaaga attgcagaag gcaaacgtcc ttttggttca
     1621 atcattcatc aggaggtggt agaagggaaa attagtgtcg cagatccaga aagtggttat
     1681 tatgtaaaaa cagaacaaga aaaacagttt gcttattctg ctcataccgt ttgtgatgaa
     1741 aatggttttg tattagatgt catgattacg cctggaaatc tacatgacag ccgaatgctc
     1801 gtgccacaaa tcgaacgtgt taaatcatgt tgtggtgtag ctgctgatgc tgcgtacaaa
     1861 acaccttgga acgccaagta tttaatcgat cgaaaactac gacccatttt tccttatact
     1921 cgacccaaaa gaagcaaaga acgttttaaa aagaaagatt tttattatga tccctattat
     1981 aactggtacc tatgcccaaa tgatcaaaca cttcagtttc gcacaactac tcgagatggt
     2041 tataaaaaat atgtatctaa atcttttatt tgtgaaagtt gtctattatt gaaaaattat
     2101 acagaaagcc agaaacacca gaaagaaata catcgacata tttaggaaga ctacttagat
     2161 gaagcagaac atttacgcca tacggtgtac aatcgcgaaa aatataaaaa aaagaaaaga
     2221 aacgattgaa cgcgttttta cagacgcaaa ggaaaagcat ggaatgcgct acaccaaata
     2281 tcgaggcttg gaaaaaacca ggtgcatacg atgcttgttt ttgccgcaat gaatctgcaa
     2341 aaattagcca tttggcagtg ggggcatatg tcttt
//
LOCUS       DACDHJ010000254         2345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDHJ010000254 DACDHJ010000000
VERSION     DACDHJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.258"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..655
                     /locus_tag="IV223_002467"
     CDS             89..655
                     /locus_tag="IV223_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8712713.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            800..887
                     /locus_tag="IV223_002468"
     tRNA            800..887
                     /locus_tag="IV223_002468"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:832..834,aa:Ser,seq:cga)
     gene            1221..2195
                     /locus_tag="IV223_002469"
     CDS             1221..2195
                     /locus_tag="IV223_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8712714.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          801 a          359 c          456 g          729 t
ORIGIN      
        1 acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag ggtacaataa aacaacatat
       61 actgcagtaa agaagtgggg gagttttaat gagaagaaaa gtctatacca aggatcaaat
      121 tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa
      181 tattgctaaa aagatgggga tttcgacaca gccgatttat ctggaattca aaaacatgga
      241 agatttaaaa aatacgttgc tggattctat ctttgaacaa ctaaaagaag atgttttccc
      301 tattgaacgt actggtgata aaatcattga tctcggtttg aactatatct actttgcaaa
      361 aaagcatcat agtctttaca tggcattgta cattgatgaa catggggacg gaaagaaaat
      421 gcatgatttt tcatacaatt atttccgtga aatagttaaa gaagatgaaa gatacaatca
      481 attatcggat gaatatgtcg atgctctgca tactggtaca tgggtagcag tgactggctt
      541 agcgtcattg atgtcatcaa acatcatgca tccaacagaa gaacaaatca ttgcactgat
      601 caaacggaca attgactcga ttttagcatt agataatcct gaaataatca gatagtgttc
      661 tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc
      721 taaaaaaagc gatttctgtt atttatctat tagaaatagg cttgacattt cgtgaaaaaa
      781 gtttaaaaat agtacttacg gaaggttggc agagtggtaa tgcaccggac tcgaaatccg
      841 gcgaaccggc taataccggc gcgcaggttc aaatcctgta ccttccttat atcaacgatt
      901 agaggttgtg aagaagctgt cctgcatcaa gtaataagaa cggccaaaca aaaatagctt
      961 ttcctatttt ttcgtttgat tgggcttatt accgcagatg caagcttttg aacaccgttt
     1021 gttcggaata aggggctgtg acaagacttt tgtcacagcc ccttattgtt gtacttttaa
     1081 cctacaatat tttttgattc cccctgagta ctatttttta taaatgctag taaatcaatc
     1141 ttctttggcc tttttttgat aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat
     1201 ttgttttagg acgggaaacg atgtgtaccg caatcactta tgcaacgaaa gaccattatt
     1261 ttggaaggaa ttttgattat gagatgtctt ataatgaagt ggtcactatt actccaagaa
     1321 attatcgttt cgattttaga aaggtaaaaa atttagataa acattatgca atgataggaa
     1381 tagcagctgg ggtatccaac tatccactct attatgaggc tacaaatgaa aaagggttga
     1441 gtatggcagg actgaatttt cctggcaatg ctgattataa agaattacaa gaaggaaaag
     1501 ataacgttgc accatttgag tttattccat ggatactagg gcaatgctca accatagacg
     1561 aagctaaaga attattggct actatcaatc tagtcaatat cgattttagt gaaaaactac
     1621 ccttgtcacc tttacactgg ttattagctg ataaagaaaa atcgatagta attgaaagta
     1681 tgaaagatgg acttcattta tatgataatc ctgtcggtgt gttgacaaat aatccgccat
     1741 ttgattatca attatttaat ctgaacaatt atcgctcgct atcaaatgga acaccagaaa
     1801 atcatttttc aaaccagatc agcttgaatg tttatagtcg tgggatggga ggattgggct
     1861 tacctggaga tctctcttct gtttctcgat ttgtaaaagc cacatttact aaaatgaatg
     1921 ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt ttttcatata ctaggctcag
     1981 tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa atacgaatat acgatttatt
     2041 cttcttgctg taacgttgat aaagggattt attattatcg tacgtatgaa gacagtcaaa
     2101 ttactgctgt aaatatgaat aaagaagact tggatcgtca tgaattggtc agttatccag
     2161 tcataaaaga gcaacagata aactatgtca attaattaat gaatagatcc gtttggtatc
     2221 atattttctg atacagtcat aattagacac gatctttagg tcgtgtcttt ttttgctggt
     2281 ttttttggaa atagcatcca ctaatatttt tttaacacga agagaataag gtagcctcaa
     2341 aaaag
//
LOCUS       DACDHJ010000255         2306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDHJ010000255 DACDHJ010000000
VERSION     DACDHJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.259"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>2306
                     /gene="esp"
                     /locus_tag="IV223_002470"
     CDS             <1..>2306
                     /gene="esp"
                     /locus_tag="IV223_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8712715.1"
                     /translation="RVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTTELVKAQD
                     EISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTGDTLKGNT
                     IGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFVRINDSLG
                     RPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDNLKTDRLM
                     YRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVSAKYEKPN
                     TQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVPYVAGIQM
                     HMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFANASPRPV
                     VIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQNTYGTGYY
                     YLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGTGHKAQLV
                     DKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSPGFDKPEE
                     GSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSNVTDSTKG
                     IVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDKPDVPLAK
                     DAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQDGSVGTTD
                     LAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          835 a          369 c          471 g          631 t
ORIGIN      
        1 agagtatttc tagaaaaaac aaagaaacga gtattgaagt acagtatcaa aaaattaagt
       61 gttggggttg catctgtttt gattggtgtt ggtcttgtct tgggaacaac tgaactagtt
      121 aaagcacaag atgaaatttc tccaagcagc ccacttgaaa cagcgataag ctccgtacaa
      181 gtaggtgaca aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat
      241 tataactttt cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc
      301 aatacaattg gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg
      361 gaaatccgtc ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat
      421 ccaaagaaag gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt
      481 agacctacaa atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg
      541 gcagaaacaa ccgatacgca aaccgctcct aatggggtaa ttacgtttga aaagagccta
      601 gatgaaattt ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga
      661 atctacttgg taagtcatca agacgatgat aaaattgtac cgggtattga tagtacgggc
      721 tactttttaa cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct
      781 gaccagttta aacatggatc agtaagtgca aaatatgaga aacctaatac tcaaacaaaa
      841 gatggttcag gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa
      901 aattataatt tctcatattc atcttccgca aagggcacgc cttggtatgc taattacaaa
      961 attgatgaac gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac
     1021 aaagtaacat atgatgtttc gtttgaatcc ggaacaaaag tagctgattt agcaatcgag
     1081 cgacgggaag gtcatgaaaa ttatggcata ggttcaataa ctaataatga tttaactaaa
     1141 cttatcgatt ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg
     1201 actaagccat tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat
     1261 aaaccatttg gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta
     1321 atccagaaca cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac
     1381 cctgacgata aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa
     1441 gaagtatatg atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga
     1501 acaggtcaca aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc
     1561 gctccaaatg aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt
     1621 gtagattcaa gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt
     1681 tttgataaac cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa
     1741 caaaccgtgg tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac
     1801 ttacctgaag atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat
     1861 agtacgaaag gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag
     1921 tttgctgtaa aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca
     1981 actccagaaa cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta
     2041 gctaaagatg ccttttcaat ttcagatgtc tataataagg acttcggtaa tgcaagtgtt
     2101 gatgcaaaca ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg
     2161 attactggaa caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat
     2221 ttagcagtaa ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc
     2281 taccacaaag taaccttcac cgcagg
//
LOCUS       DACDHJ010000256         2266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDHJ010000256 DACDHJ010000000
VERSION     DACDHJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.260"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(592..957)
                     /locus_tag="IV223_002471"
     CDS             complement(592..957)
                     /locus_tag="IV223_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712716.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1033..1278)
                     /locus_tag="IV223_002472"
     CDS             complement(1033..1278)
                     /locus_tag="IV223_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712717.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1348..1602)
                     /locus_tag="IV223_002473"
     CDS             complement(1348..1602)
                     /locus_tag="IV223_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712718.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1766..2089)
                     /locus_tag="IV223_002474"
     CDS             complement(1766..2089)
                     /locus_tag="IV223_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712719.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          858 a          372 c          390 g          646 t
ORIGIN      
        1 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
       61 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      121 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      181 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      241 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      301 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      361 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      421 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
      481 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
      541 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
      601 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
      661 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
      721 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
      781 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
      841 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
      901 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
      961 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1021 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1081 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1141 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1201 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1261 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1321 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1381 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1441 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     1501 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     1561 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     1621 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     1681 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     1741 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     1801 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     1861 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     1921 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     1981 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2041 ctaaatcgaa taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat
     2101 aaaagaataa cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg
     2161 acacaagtaa aagaaataag aataacaata ttattttact tatattttga aacttgtgag
     2221 agtttgtcat tcgtgctata gttgaaaagt aacaaaaata aatttt
//
LOCUS       DACDHJ010000257         2262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDHJ010000257 DACDHJ010000000
VERSION     DACDHJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.261"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..1931
                     /locus_tag="IV223_002475"
     CDS             69..1931
                     /locus_tag="IV223_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712720.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1931..>2262
                     /locus_tag="IV223_002476"
     CDS             1931..>2262
                     /locus_tag="IV223_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712721.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          812 a          338 c          447 g          665 t
ORIGIN      
        1 agtgcaaaca tttcatattg tcgttagttt agaaaacgct tatattagca atgtaaggag
       61 agatgataat gcagcagcta acaaaaagag aaaggcagct tttgatttac ttggtggaaa
      121 tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga aaatgttagt gttcggacga
      181 taaaatacga tctagatgat ataagagagt ggctgaatga gcggaagcaa gtcctatata
      241 gcaaacgtag tcagggaatt tggatgaata ttagtagctc tgatcgaatc aaattaaaaa
      301 gcgaattgat ggatgttaat cgttttgaaa tatttgcaga tcaaagtctc cgaatcaaac
      361 gtttactaat tatgctttta gtaacaaaag aagctattac tggaatgaaa atggccaacg
      421 cattagatgt cagcaaagat acgatcatga atgatttaga tgtgctggaa aataattttc
      481 ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg tttttggatc ataggagaag
      541 aacgtctagt tcgattaacg attgaagaga ttcttcaaaa agagttcacg gattatgaca
      601 tttataaatt aatgtcattg ttattgaatg gcggtgagac tgaatacttt gaaatgtatt
      661 ctgctactgc tacacctatc caagaggtat ttaatcaagt aatcattcga atgcgacact
      721 tactggaatt tgaaaatcta gagaaactga attacgcgga attactaaat attctgattc
      781 gagtgacaat tgctaccgtt cgcttacgca aagaggctac gattggtcgt tatcaattgg
      841 ttgctgaaca agaattagac aaaaacgatc tttcttatcg cgtcatgcag caggtctttg
      901 attattatca gttgccactg ttcgaggatg aatatcatta tatctatagt gatacattcg
      961 acacgattca ccagcaagac gtcatgaaac tagttgagaa tgtgatttat aaagtaagta
     1021 aagcaatcaa ccattctttt tatcaagatc cacaattgtt gacgaatctg tatgctcatt
     1081 tatccatgcg attatcgaga aagaaaaaat ttgtcaatga atataatcct tttaaagaag
     1141 atatcaagca gaaatacccg gaattatttg atgcgatcaa gcaagcagtt cgacaagaaa
     1201 taagtgaaca aaatattctg atcaatgatt ctttcgtggc atatattgcc ttacactttt
     1261 tggtttccta cgaaagagaa gaagatctac gaagcgttag agccgtctac atttgttcaa
     1321 cagggttggg tgtgactagt ctgatcaagc aaaagatcaa tgaagaatta aataatatca
     1381 ctatcgtggc atttgcttca gtattgaatg cgcaagaaat gattgctacc aaagatcctg
     1441 atttggtgat tagtattttt ccaattaatg gcatcgatcg tcctttcatc aaagtccatc
     1501 cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat aataaaaaaa atacttgctt
     1561 cttccaatca gcaggaaagg cataacttga atcttagaga aacgtccatc aataaagata
     1621 ctttagaaga ctttagcaag gaattgattt tgaaagctta tacgatttat gaacgtttac
     1681 ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga agcttttttg cttcatgtat
     1741 tcttaatggt tcatcgtatc acatttgatc aacaatatat ggcagaaggt cgtcaagtag
     1801 accagattat tttagacaaa ggaacactgg taggaaaaat cgaagcagtt tttgcagaaa
     1861 ataatttagg aatcaatcag tcagaaatca gtgcattatt ctcatatata aaagaaggag
     1921 cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt atcgaggaaa gtcaaaatcg
     1981 ggaaaagtta aaacaagtga ttgatctaat tgaagagcga ttagtagaac atgctattgt
     2041 accaaccgag ttgcagtgga ccattctaat taatcacttg aatgagatgc tgaaacgttc
     2101 tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg ttcaaagaag tatccaaaga
     2161 agccataact atttctgatg aaatagttag aagtatcggt aatttaaccg atgatgaaat
     2221 atatgttttg tcgattcatt ttgaaacagc aaaaaataat ta
//
LOCUS       DACDHJ010000258         2197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDHJ010000258 DACDHJ010000000
VERSION     DACDHJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.262"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..1527)
                     /locus_tag="IV223_002477"
     CDS             complement(1..1527)
                     /locus_tag="IV223_002477"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8712722.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(1542..2189)
                     /locus_tag="IV223_002478"
     CDS             complement(1542..2189)
                     /locus_tag="IV223_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8712723.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT          594 a          456 c          410 g          737 t
ORIGIN      
        1 tcaatcgttt aattttccac gttcacgata ccagttatct ggttgccaga tccattcgaa
       61 tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc attgatccag ctttgtagtt
      121 gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg acatccacga tatgccaaga
      181 ttgagcgact tcttcccaat gagagaagtt cgttccatca ccattcaacg catctaatag
      241 taatttttcg taagcttctg ggctattttc aaccatttct gcactatttc taaattccaa
      301 tttgattggt tccgtttcaa atccttgccc tacttctttc ccattcaagg ataaagaaaa
      361 tccttcagtt ggctgaatat agatcgttag aacattaggt ggaagtgtcg tatcgtcaca
      421 tggttcatcg acagatgttt tgaatacatt aacaggaact tgtttgaata cgatgttgat
      481 ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca
      541 acggaagtta tcgatagtga atttgcctgc gacgaatgtt tcagtctctg attgagcatc
      601 cactttgtct tcttcgcgat agcccttgaa ttgtacacca tccaattcac cttgagcata
      661 ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta taaatgcgga tagctttcaa
      721 tgctttgatc ttttctgtac ggatttcttt ttcggaaaat gctactggcg gttccattgc
      781 aagtaatgct acaacctgta aaatatgatt ttgcaccatg tctttaagtg cgccactatg
      841 atcgtagtag cccccgcgat cttctacacc gaggttttcg ccaaaagtaa tttggatatt
      901 gtcgatatag cgattgttcc attgagcctc aaaaatatta ttggcaaaac ggatagctga
      961 gatgttttgg atcatttctt ttcctaaata atgatcaatc ctgaaaatat cttgttctgg
     1021 gaatacttct ctgatttgct ggttcaattc aaaagcagac tggtaatcag aaccgaacgg
     1081 tttttctatg atcaaacgat tgaagccatt ttttgtcata atattttgtg atttcaaatg
     1141 agaaacgatc gttccaaaaa attgaggtgc catagccaaa taatagacac ggttaccgtt
     1201 taattgatag tgtccatcta atcggtcaga caattctttt aatgtctgat aatgcgttga
     1261 atcatttaca tcatgggatt gatagtaaaa atggcttgca aaggtagttg cttcttcatc
     1321 tgaaggattc aatgcacgga ttgtttcttt gacaacttca cggtagtgct catcgctcca
     1381 gggacggcga gcggttccga ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag
     1441 gcggaacaaa gacgggtaca atttacgctg ggctaggtca cctgtaccgc cgaatatcgt
     1501 aaacaataca tttttttcat tagacatgtg gatacactcc tttatatttt atcgacaaaa
     1561 atcgtgctgg ccgccttaaa gctgacgggg attttctttc tatcattttg gatagtgatc
     1621 ggtccttcat aagctgcgat gtcaacgatc tcatattctt cgttgatatt caggtcaata
     1681 gtgactaagt aatccaacaa ttctttttca tcaagcactc gtgcgatacg cacttttgta
     1741 ccgacaggat aggactccaa tgtttgtcgg cgttcttcat ggacaacttc ctctttttta
     1801 ggaatcgatc ctccatgagg acaatgttca ggatgattca aatagttttc taaatgatct
     1861 gcaagatgtt ccgaagtcac atgttccaat acttcagcat catcatggac ttcattccag
     1921 ctatagtcta aatgttcaac taaaaaaact tcccataagc gatgtttgcg aatcagactg
     1981 ctagcttttt gcagtccgct tgcagtcaat tgtacacctt gataagggga atgttctaca
     2041 agctcttctt tcacaagttt agaaatcatt tcactgacag atgcagctga gacatctaga
     2101 ctagatacga tttgtttatt gttgactttc gttgtatctc cacctagttc taaaatgatt
     2161 ttcaaataat cttctcgatt tggtgtcata ttcgatc
//
LOCUS       DACDHJ010000259         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDHJ010000259 DACDHJ010000000
VERSION     DACDHJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.263"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            163..429
                     /locus_tag="IV223_002479"
     CDS             163..429
                     /locus_tag="IV223_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712724.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            487..999
                     /locus_tag="IV223_002480"
     CDS             487..999
                     /locus_tag="IV223_002480"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8712725.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            996..2033
                     /locus_tag="IV223_002481"
     CDS             996..2033
                     /locus_tag="IV223_002481"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8712726.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          787 a          410 c          449 g          545 t
ORIGIN      
        1 gctcactccc cgaccaaagt ttgtgagcaa aataaacagt cgatgtttgt ttcttaattg
       61 tatatcttga tattaaacga tttaagatta ttcttcaaga tatatattcg caaatgtaat
      121 tggaaaagcg acttcttaaa caaaattaag gagtcgcttt ttatggcaaa aataacgaaa
      181 aatgagcaag aaaatttggt ggaaaattac acaaaaaaac cggcgtactc taatagccgg
      241 ttagacgcac atttcgtgtg cacttctaat ccgaaattga gctttgacgc tatgacgata
      301 gtcggaaacc ttaataaaaa cagcgctaaa aagctatccg attttatgag tttagatcca
      361 caaattagac tttgggatat acttcaaaca aaatttaaag ctaaagcact acaagacccc
      421 ctatgttagg ctagttgtcg tataggataa taagttataa taaagactat acggaggatg
      481 attaccatga caaataaaaa caatgaacgt tactctaccg aactgaaagc gacagccatc
      541 aagaaaatga tgccacccga aaataggggg attcgccaat taagtcaaac tttgggcatt
      601 ccagaagcga ctctttgttc ttggcgtaaa aaagctagaa ttcaagggaa tactacaccc
      661 ggaacgaaaa aaaatgtcga aaaatggtca agtgaagata aatttttaat tgtgatggaa
      721 tcattctcaa tgaaccaagt agaacttgcg gaatattgcc gaaagaaggg cttatttaaa
      781 gaacaaattg aggcttggaa gaaaacgtgc ttatctgcga atgaacagga agaaaatcga
      841 acgagagaac ttgcaactga actaaaagaa gagaaaaaac aagcgcgtca attagagaaa
      901 gaattacgtc ataaggaaaa agcattagcg gaagccgctg ctctcttatt actaagaaaa
      961 aaagcacacg cgatttgggg ggaccaagag gaagaatgat ttcatcttca gatcgcaaac
     1021 aagctgttga acttattcaa gaagccaatc gcaatggggc ccgtctcacc tatgcctgta
     1081 atgagctgaa tattagcgtt cgaacctacg aacgatggac gcgtgaaggt acgatagcgc
     1141 atgatcaacg ccctttggcg aaacgtcctg tacccaaaaa taaactgacg gatcaagaac
     1201 gcgaaaaaat aatagaaacc gtttcaaaaa aagaatttat gaatttgcca cctagccaaa
     1261 ttgtaccaaa acttgcggat tgctcgattt atattgcatc agagtctagc ttctatcgtg
     1321 tattgcgaga aaaaaatatg caacatcacc gtggccgaag tcaagtttca caaaaacgca
     1381 taccgccaag tcaccttgcg acgaagccaa atgaagtttg gacttgggat attacttggc
     1441 taaaaggtcc aataaaagga ctcttttacc ggttatatct catcattgat ttatttagcc
     1501 gtaaaatggt aggctgggag atttgggaat cagaagaagc tcgtcatgcc gaaaaactta
     1561 ttaaacagac ggtgcttaag gaacgtatcc aaggacagcc gttggtttta cactccgata
     1621 atggcagccc tatgaaagcc gcgacttttc aagtcttact cgaaaaatta ggaattcaaa
     1681 gttcttattc aagaccacga gtgagtaatg acaatcctta ttcggaagcg gctttccgaa
     1741 cgttaaaata tcgaccagaa tatcccaata acggctttaa aacaatagat gatgctcgaa
     1801 aatggacgaa agagtttgtc gattggtata acgattctca tttgcatagt gggattcaat
     1861 ttgtgtctcc tgcagcgtgt catgaaggac gacatctcgc tattttagag aaacgaaagc
     1921 aggtttacga ggacgctaaa aagcgccatc ctgaacgctg gtcaaaaggt gttcgaaatt
     1981 ggactccaca tcaatcggtg gcgttgaatc ctttaaaaga aacagaaagt taacaggagc
     2041 ataatctttc aagattatca aataaccctc gtcacccaat aggggaaaaa gttattcttc
     2101 ccctattgga taacgaggcc aaccgaccat agggaggttc tgctttttga taggtggaaa
     2161 ataaaaatac gacaactata ttgacaaaca c
//
LOCUS       DACDHJ010000260         2157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDHJ010000260 DACDHJ010000000
VERSION     DACDHJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.264"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..1433
                     /locus_tag="IV223_002482"
     CDS             6..1433
                     /locus_tag="IV223_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8712727.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1525..1890)
                     /locus_tag="IV223_002483"
     CDS             complement(1525..1890)
                     /locus_tag="IV223_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8712728.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1887..>2157)
                     /locus_tag="IV223_002484"
     CDS             complement(1887..>2157)
                     /locus_tag="IV223_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8712729.1"
                     /translation="LFKLFDWSHVKKNYAERNTRNADTRKLLASYILLSLEVLVVADI
                     IESVIKPTWTDILKLALIIIIRTVISYFLNQEIEDKEKQRRGEVG"
BASE COUNT          703 a          411 c          360 g          683 t
ORIGIN      
        1 ctactatgat tgaagaatat attgagaaaa atattttaag acagctgttt ttatgtgggc
       61 aattctatgt aaataaagaa gtcaatctag aaaagctctc caatctcctg catgtttgca
      121 aaaccacttt actcaatgat atcaataaca taaaaaaaga atttgaagaa cagatcgctt
      181 atacacatcg ggaaaaggac cgttacactt tatatttttc agaacatata cctcgatgca
      241 agatcatgca gcaattgtct caaaattcgt tgtttttaaa aacttgtctg ctttatttgg
      301 aagaagatga acctgattat cttcaattaa ctgaatgtga attcatctct gtcagcaaag
      361 cttattcatt aaaaaaacaa gtattagctt actttaatga ctgcgggatc gaaatcgacc
      421 gctactcccc tcgattcact gaaatggaaa gacgtctgct gcttcttaat gtgtcgtatc
      481 gtttaggcgg ttttaattct tgggagctac ctgaatcgtt ttttgaacgt gccgatcgtt
      541 tcattgaatc cgtaacggaa aatagtggca gattttatga taaagaaaat aaagaaatct
      601 tatcgattgg ttttgctatc agtttcctac gccagcaagt gtgtgctgtg acaatagatt
      661 caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa tcagcatggg
      721 aaaacactga tttccaaacc tattataaaa aagaagaatt cgcctttatt ttgacactat
      781 tcaatctttg taattatggt ttccattctt atcagttgat tgcggaagac tttcaacagc
      841 tacaccaagt gtttattgac aatactcccg aaataaaaga actagttgct acctttgaat
      901 ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg attcatctga
      961 tgcgttcggc ttgggataac tatcaattat tcatgccaga aaaattttat cttttaaatg
     1021 aggaacagac aaatttatat aaagaagtcc agacaatttt ttccagctgg tccagccagt
     1081 taccttatga tctacggttg aatcctaatt gcatgcgggc ttttgtgatc gaattgagcg
     1141 gaattctccg tctgacaaaa gaacatctga ctatctatat cgtcacaaat tcggatgttc
     1201 attatctgat ttatcgggaa gccttggaag ctgtaactac tttcgatttt caagttgcac
     1261 caacaattta ttcaagtata tctgatataa agaagtatgc gcagcagtcc tcaaaccggg
     1321 tattatgtga acgtacatta tatacaccag atgcggtcca atatgaaaat attattccta
     1381 tttcgatcaa tacgatcgat cgagctatca tttcagctgt gcaaaacaaa taaggggctg
     1441 tgacaaaaat catgtcacag cctcttattt gttgaagatg cccatttctg taacaatctc
     1501 ttgatacacg tcaggctcac aacctcactt attttggaca tctaggggtc cgatctcttt
     1561 atttaaaaaa taagaaatga tggttcggat aactaccaac gccgctagtt ggaaaatatc
     1621 ctgcaaggta ggtttgataa tcgattcgat gatcccagct gaaacaagaa tttccaaact
     1681 tagaagtata tagcctccca acatcttttt aatagcattg ttctcgctta ctcggtcgct
     1741 ttgcactttt gtatggacaa ataaatattt caaaaaatcc ttgattgata gacaaacacc
     1801 ccaaaccaat aaacagatag caaagatatc aagtcccagt acaagttttt cgaaatatgg
     1861 aagaagaaac tgcatcaact ctgtcgtcat cctacctctc ctcttctttg tttttctttg
     1921 tcttctattt cctgattcaa gaaataagaa atgactgttc gaatgataat aataagtgca
     1981 agcttcaaaa tatctgtcca agtcggcttg ataactgact cgatgatatc agcaacgact
     2041 agtacttcca agcttaacaa aatataactt gctagcagtt ttcttgtatc tgcattacga
     2101 gtattacgtt ctgcatagtt tttcttgaca tgagaccaat caaaaagttt gaataga
//
LOCUS       DACDHJ010000261         2146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDHJ010000261 DACDHJ010000000
VERSION     DACDHJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.265"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..248
                     /locus_tag="IV223_002485"
     CDS             24..248
                     /locus_tag="IV223_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8712730.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            245..1270
                     /locus_tag="IV223_002486"
     CDS             245..1270
                     /locus_tag="IV223_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8712731.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            1460..1533
                     /locus_tag="IV223_002487"
     tRNA            1460..1533
                     /locus_tag="IV223_002487"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1494..1496,aa:Ile,seq:cat)
BASE COUNT          702 a          364 c          420 g          660 t
ORIGIN      
        1 actttattta gggagagaag aaaatgaata ataaaacgtt cgaagtacta aaatggttcg
       61 cactggtaat tattcccgca ctagctactt tcgtggggtt agttggtaaa gcgctcaatt
      121 ggcagtacac agatatctgt gttgtcatca ttactggttt tggcgcgttt ttagggagtg
      181 tgttgggtgt atcaaatcga acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa
      241 acaaatgaaa aagaaaatta ctattactgc gatgagcctg ttaacggctc tttttttatt
      301 acccattaat acgtttgctt atactattaa tgacgagtat aatttagcgc cgaatcaagg
      361 agactccaga ttagcaattc ctaacaaaat tattttgcac gaaactggaa tagatgcacc
      421 agcaagaaac gtagccgcca acatgaaaaa taattataac ggaagcaatt cttatactac
      481 agatgttatt ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg
      541 gggagctggt aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga
      601 taaagcattg tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa
      661 aaaatatgga attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat
      721 ttctcatttg tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt
      781 atcgcaaatg ggagttagca aagaaaagct tgcttatgat ttagctcatg gatttaccga
      841 tgaaaatcca acaacttctg aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc
      901 taatcctacg ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga
      961 aaacgcaaac ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt
     1021 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
     1081 accagatgta aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa
     1141 tatgcgtaat tttcctagta agaaagtcta tgtaatgatg cgggcaacga atgatccaga
     1201 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
     1261 taaacgataa aaatagctcc tcgttgagga gcagtacata actatattga caactataaa
     1321 aattattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aaatagtcac
     1381 actccaagct atgcgataac aggtttgttg ccacacattc tactggttga ttgtttatgg
     1441 ctttatgtgg caacaaccag tacccttagc tcagttggtc agagcagacg gctcataacc
     1501 gtccggtcgt aggttcgagt cctacagggt acattaacgt agccatttga atcgttctgt
     1561 gttagaattt ttttgaagag tattatacaa gctaaagctt ttcttcattg ccactcaaat
     1621 gagtggcttt tttatgtatc cttttatgga ttaatgaaag gatgtttcac atagttatac
     1681 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttaaatt
     1741 gtgagataat aataaagaag agtttaaagc gcaccccaaa ccacttcccc ataagtgtgt
     1801 tacgctttaa actcttttat atttgaagcc attaaaaagc ataccatatt tttgaaaaaa
     1861 agtgagaaaa aaggcttaca attggagtgg tagttaatta gtgacttatt tttgatttta
     1921 tagcactgat actataaaat atagatatca tcatattaca caatcttaat actaacttaa
     1981 aaaatatctc ctttcacaag tatggtgata aaattcgttc cgggctacct ttttaggtag
     2041 cctactttaa tctttatacc tttctggatc aacgaaagta tactttatat agtcataacg
     2101 ccgatgatcg ctacgtgcgt ccggcacgtc agtcatgaat cggcta
//
LOCUS       DACDHJ010000262         2105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDHJ010000262 DACDHJ010000000
VERSION     DACDHJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.266"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            92..1723
                     /locus_tag="IV223_002488"
     CDS             92..1723
                     /locus_tag="IV223_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8712732.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1726..>2105
                     /locus_tag="IV223_002489"
     CDS             1726..>2105
                     /locus_tag="IV223_002489"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8712733.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSST"
BASE COUNT          739 a          291 c          442 g          633 t
ORIGIN      
        1 tatttttagt tttttcttat tgacagcgat tacattcgat tttatactac aattgtaacg
       61 gaaactaaca tatcagtatg gaggggtaaa tatggttttg attaaagact ggcaagattc
      121 tatcgaagga ttgaacaaaa tgcaagttca actaccgtat tttaatgtcg aagcggtcaa
      181 acaagaaggt cagaaacatc cagtgtggct tcattttggt ggaggaaatc tttatcgagg
      241 atttcatgca gaaattgctc aatgcttagc agatcaaaat gaattagaat ccggagtagt
      301 tgtgtgtgag acatttgatg aagaagtagt agaaaaagct tatcatgact acaacaatga
      361 tattttggag attgttatgg gtgaagatgg tgaactcaaa aaaagacttt tagcagtaac
      421 agctgatagt ttatattgca atccaaagaa tataacggac tatcaaagag tggttaaata
      481 ttttgaagat cctacattac agtttacaac gtttactatt actgaaaaag gatacagttt
      541 aaaagattct aaaggacaat ttttttcggt agtggaagca gatataaaat ctggacctac
      601 caatcctcaa catacgatga gtattgtgac atccttgttg tatagcaggt ttaatgctgg
      661 agagttaccg attgcgatgg tttctactga taatttctca cagaatggtc aacgttttca
      721 agatagcatt tttatgattg cagaagcttg gcaaaaaaaa ggatacgttt ctgaaaaatt
      781 tattgcttat ttaaaggata aagaaaaagt gacttttcct tggagtatga ttgatagaat
      841 tacgccaaat ccttcggaaa gtgtcaaaat ggatttattg aaggcgggtt tcgaagactt
      901 agatataatt catactgcga aaaataccaa tatcgctgct tttaccaata ctgaaaaagt
      961 ccattatcta gtcattgaag atagttttcc aaatggtcga cctaaactgg aagcggctgg
     1021 tgtgattatg acagatagag aaacagttga taaagcagat atcatgaagg ttacgacatg
     1081 tttaaatccg ctccatacgg cattagcaat aacaggttgt ttgttggggt ataaatcaat
     1141 tcaggcagaa atgcaagatg atgatttggt tggcttaatt aaagggatag gttatgtaga
     1201 aggcttacca gtagttgaaa gtccaggcgt tattgatcca aaacaattta ttgatgaagt
     1261 aatcaataaa cgtttgccta atccctatat tccagataca ccgcaacgaa ttgcaacaga
     1321 tacttctcaa aaagtagcga ttcgctatgg tgaaacaatt aaaaagtatg tcgaaaaaga
     1381 cggaaatgca gaaaatctaa catttattcc tatcgctatt gctgcttggt tacgttattt
     1441 attggcagtc gatgatcaag gaaaaaattt tgtgccaagt cctgatcctt tattggtaga
     1501 gttgcaggaa aaattctcag tattgaaatt aggtgaacaa gatcctgcaa agattcattg
     1561 tgcggtacag gaaatcttag aaaataagga aatatttggt ttagatttat atcaagttga
     1621 gataggaaag aaaattgaaa aaatcttttg cgaaatgcta gtaggtcctc aagcagttcg
     1681 taaaacaatc aataaatata taacagaagt tggaggaaag taactatgga aatgactttt
     1741 agatggtatg gggcaaatga agaaaaaatc tcactacaag aaattcgtca gatcccagca
     1801 atgaaaggaa ttgttggaac attaatggat ataccagccg gagaggtctg gccgagagaa
     1861 cgaattaaag ctttaaaaga tgaagtcgaa gccaatggcc ttaccttgaa agttattgag
     1921 agtgttaatg ttcatgatga tatcaaaatt ggtttgccaa cacgagatca atacatcgag
     1981 aattataaac agacgattcg caatttggca gaatttggga ttgaggttat ctgttataat
     2041 tttatgccag tctttgattg ggtaaaatca gatttggatt atcatttaga tgatgggtct
     2101 tctac
//
LOCUS       DACDHJ010000263         2089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDHJ010000263 DACDHJ010000000
VERSION     DACDHJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.267"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(619..909)
                     /locus_tag="IV223_002490"
     CDS             complement(619..909)
                     /locus_tag="IV223_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712734.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1117..1809)
                     /locus_tag="IV223_002491"
     CDS             complement(1117..1809)
                     /locus_tag="IV223_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8712735.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          592 a          390 c          310 g          797 t
ORIGIN      
        1 ttaaatacct tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat
       61 atccagaaaa atttttctta gttaaggacc tatggattgc agtaatgcag aattcgtcga
      121 ataaactgcc aaagacatac aatcatattg cttttaaaat tgacgaacaa gatatagatt
      181 cgtttgtctc aaaaatccaa atgttaggtt taactgtcga accaggacgt tctcgtgtga
      241 aaggcgaggc ttcttcaatt tacttttacg actatgataa tcacctcttc gagttacata
      301 ccggtactct ccaggaacgt ttaaaatcct acaattccac tacctgagac tagtaatgac
      361 tagtctcttt ttatggcctc tgatcccccc attgagcgaa tactaatagg gcttcgaatt
      421 gatctgttca atctttaagc aatatccctc aaatgagttt ggaaagttta gtgattcatc
      481 acaaatactt aaatacatgt agttatatat agattgaggt tcttgtgagc gtatgctcgt
      541 gtgggttcaa atcccatcgt ctgcatccat ctttccaaaa caagcggcta ggacattttt
      601 gtctcagccg cttgttttct agtttattct ttcatcttgt cggacctttt ttcttgggta
      661 tagacttttc tcatattcta ccacaatttt gggtttccct aacacatgat aggatctcac
      721 aacaaattgc tttctattat tataataacc tgtaacgact aacttcatcc cttcgtctac
      781 ttcagctagg aagttcaaac tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa
      841 acggatcaac ggtctggcac taacttgcaa agtacggatc ttatctactg ttccatattt
      901 tttctccata accttcacct caataccatt atacgaacat acattccctt tgtaaagaag
      961 aaactctgtt ttatcttttt gactttttgt ctattacctt tcttaaaatc atattggcac
     1021 ttcctgcgaa gatgaaggac atgtactaaa aaaaagaggc agcagtatag attactgttg
     1081 cttctttact aatttaggtt tacttgttaa gaaagattat ttacgcaaat gttcttcaac
     1141 aatttgctga gatttatcag ctgtatcttc cacttcatct tttgcttttt ctaacttttg
     1201 aatatattgt ttcaagcttg agttttcagc agttgcttcg tctaatttct tttgtgtatc
     1261 tgttaactga gtttctacat ttgttttttc ttgtgtcagt gttgcgattt gttgattcaa
     1321 accatttaca gtattttgta attctgaaag ttttgcttgt gcttcttgta gtcttgctgt
     1381 ccaatctgca ttcagatgat tgatttcatc atttttttgg ttgatctcat ctagtttgac
     1441 attgatttca ttttgtttgc tttgtagttg tgcatccttg ctgactaatt gactttctaa
     1501 gttttgtttt tctgctgccg attgattttt ttggttttct aattcattta cttttgtttg
     1561 gtattcttgg acatctgcct ttgaatcttg caatgtctgt tctttttgtg ctagtcgagc
     1621 ggtaagatct gtaagcgctt gctctttatc tgtcaatgta tgatcaagct gtgtcagatt
     1681 ctcattgatt gtatcaatat ctgtctctcc accccacaaa ataatttcat ttgcgtaccg
     1741 cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca atcccaccag taataaattt
     1801 ctttttcatt tgcatagctc cttaatccaa tacttttaca tgctaaaagt aagtgatctc
     1861 aaacacaatg tcaagcgttt ttaaattaca tgtgtaattt taatataaat actatcaaat
     1921 actcatttaa tcctcctttt ttatctattt aaaaaatctt ttttatagag aaaaatgcag
     1981 gttaatactt tcttaaataa atatttatgg tgaacaaaaa caaaaaagca agttttatta
     2041 aacgtaaccc ttttcatttc atagattact gtatttatta caaaaaacg
//
LOCUS       DACDHJ010000264         2071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDHJ010000264 DACDHJ010000000
VERSION     DACDHJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.268"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(92..2020)
                     /locus_tag="IV223_002492"
     CDS             complement(92..2020)
                     /locus_tag="IV223_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8712736.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT          635 a          402 c          378 g          656 t
ORIGIN      
        1 gaaagttaca aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata
       61 aaataaacaa aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt
      121 atagtggaca agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta
      181 aacgcaagcc attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag
      241 gtagatttaa aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa
      301 aaataatatt ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa
      361 atttatttaa gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc
      421 cttgccccat agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat
      481 caccaaccat cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt
      541 cttccggagt acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg
      601 ctgtgccaga atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg
      661 aaattacttg ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact
      721 gctgatcttt agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt
      781 ccgcaatctc tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc
      841 attgcgcacc taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt
      901 attggatttc ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat
      961 gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga
     1021 aaaagctgtc agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa
     1081 tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt
     1141 ttgcagaatt tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag
     1201 gagaagccac aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta
     1261 ggaaaaagtg ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat
     1321 tattttcgat tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta
     1381 ctaattggat aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga
     1441 tgggatttcc ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag
     1501 gcacagattc accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc
     1561 catctatcgg aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag
     1621 aaacatctac ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa
     1681 gattcatcaa agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac
     1741 cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg
     1801 caccaatagc agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt
     1861 ctttcaagcc ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa
     1921 gtggggtgat ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc
     1981 agccgattgt tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc
     2041 aaaacaagga tagcataata aaaatgtaat t
//
LOCUS       DACDHJ010000265         2068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDHJ010000265 DACDHJ010000000
VERSION     DACDHJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.269"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..1897
                     /gene="asnB"
                     /locus_tag="IV223_002493"
     CDS             2..1897
                     /gene="asnB"
                     /locus_tag="IV223_002493"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8712737.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT          726 a          349 c          415 g          578 t
ORIGIN      
        1 aatgtgcggt attgtaggtt ttgttaacga caaggacaac aaaaaaacaa ttattaatac
       61 aatgatggat cgaatcgttc accgtggacc taatagttca ggagaatata tcgataaaca
      121 tgttgcttta ggattcagaa gattaagtat tattgactta gagggcggta ctcagcctat
      181 ttacaatgaa gatcggacaa agatcattat ttttaatggc gaaatctata attaccagcc
      241 tttgagagaa gaattgattg cagctggcca cgtttttcaa acacatgcag atacagaagt
      301 ccttttgcat ggttacgaag aatggggaac agaattattg caaaagatcc gcggaatgtt
      361 tgcttttgct atttgggata atgaaaagaa tgagttattc ggtgcaagag accactttgg
      421 gatcaaacca tattattatg cagaaatgaa cggaacattt atgtttggtt cagaaatcaa
      481 aagttttctg cctcatcctg attttaacaa agaattgaac aaagaagcgt tgaaacctta
      541 tatgactttc caatattctc cattaaacgg cgaaacattc ttcaaagatg tctatcgttt
      601 gcctgaaggc cattattata cgtataaaga tggaaaaata gacattcagc aatattggga
      661 tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa tggatcgaaa aaatcgacga
      721 aaccgttcaa gcttctattg aagcacatac ggtcagtgat gtagaagttg gttctttctt
      781 atccagcggg gttgattcca gttatgtaac ctctgtattg aaaccagatc attctttctc
      841 aatcgggttt gatgataaaa cctataatga agcgattgaa gccagaaaac taaccgaact
      901 attggactta gataatacag cagcagtgat cgacggagac atgtcgttca aagcattccc
      961 tttgatccag tatcacttag atgaacctga ttcaaatcct tcttgcgtac ctttgtactt
     1021 tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa tctggagaag gggcggatga
     1081 actgtttgcg ggataccaaa cgtatggatt ccatacgaat tcaaaattca tccgtgtgat
     1141 tgctcaagga ttgaaaaaat taccaaaagg aacacgctat aatttaggac gtaaaatcgg
     1201 aaaaatgaaa aatttccatg gacggatcca tttatatgaa tcacttgctc ctgcaaaaga
     1261 atattttatc ggtcatgctc gagtgttcga agaatcagaa gcatcagaag tgttaacacc
     1321 aaaatatcaa acagcaccat ctgtggatga aatcatgact gttcattacg agaaaacaga
     1381 aggaatcaaa gatgaagtca ataagatgca atatgtcgat cttcatcaat ggatgccaaa
     1441 agatatcttg ttaaaggcag ataaactttc tatggcaagt tctttggaag tccgagtacc
     1501 tttattggat atcgaagtca tgaaattggc tcaacaaata ccaagtaagt ttttattgaa
     1561 tcaaaacaac acaaaagata ttttcagaca agctgcgaac aaacatcttc cggaagaatg
     1621 gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa gcttggttga aagaagaaca
     1681 cggctacgaa caagtcaaag cattattcga agcagatttt gcaaaagaat tttttgatca
     1741 agaaaaaatc atgaagttgc tagatgacca tcacgaagga cgtaaagaag agcaaagaaa
     1801 aatctggaca atcttcagtt tcttgacatg gtataaagtc tactttgtag atgaaacgat
     1861 tccgcaagca gaagcaatcg actatgtgac tgtttgatga aaatcaaata tcaataaaaa
     1921 agaggttttc tcctaccgtt taacactgag tgataagaaa caatttcaaa aaacagctct
     1981 tcccgtttta tagatattat gacttatggt tcggagttgg cagaggaaca ccattgtttc
     2041 gaacataagg gggttgtgac acgatttt
//
LOCUS       DACDHJ010000266         2051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDHJ010000266 DACDHJ010000000
VERSION     DACDHJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.270"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..497)
                     /locus_tag="IV223_002494"
     CDS             complement(<1..497)
                     /locus_tag="IV223_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8712738.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIG"
     gene            832..1266
                     /locus_tag="IV223_002495"
     CDS             832..1266
                     /locus_tag="IV223_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8712739.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1289..1609
                     /locus_tag="IV223_002496"
     CDS             1289..1609
                     /locus_tag="IV223_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712740.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1750..1941
                     /locus_tag="IV223_002497"
     CDS             1750..1941
                     /locus_tag="IV223_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712741.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          718 a          321 c          448 g          564 t
ORIGIN      
        1 ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat
       61 tcttttgcag agcttgtact tgctggagta gcagcagttt ctgttacagc aggagcaggt
      121 tgtgtttcag caacaggcgc tgcttgtgtt tccgtttgaa ccgtttcttg tacaacaggt
      181 tgttcagctt gaacaggttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
      241 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gtagatcagg
      301 ttaatatcag aaattgaatt tgattctgca atggcattga tcaaggagtt atcaccaacg
      361 tatttttgag aaatcgttga tagagtatca cctgattgaa cggtataagc ttcgtccgca
      421 tgagcagtcg ttcccatgaa gaatgcagcg ccggcagcca aagttgttcc aaaaagtaaa
      481 gttttaagtg atgtcatgaa aaatctcctt tattcgttaa aaagttttat ttgttgtgtt
      541 caacgaggaa tactttaaca cgtgaaaata tttcataggt aaaagaagca tgacaattta
      601 tgacaaatcc acgttttttg taacagaatg atgttcgctc gatgtgaaaa atgatgagga
      661 atttcacata atgcagaaat aatgggatta gaacaggtgt tctgatgaga aaagaaatgt
      721 ataaataact tgaaagaaat tgcttttgtt catcatttta tttacatatt gcgtatattt
      781 tttgtatttt tgcttgagat gttacaataa aagaaaaagg aggcggttcc aatgagtaaa
      841 agataccaga acatcatggt agcagttgac ggatcaagac aatcgatcaa agcgttcgct
      901 gaagcccttg acttagcaaa ggacaatgaa gcccacttat ttatcgtgtc aatcatcaac
      961 aaagttgagc tgacccatag tgcctatgcc ttttcaaaaa tctatgcaga tgaaaaacaa
     1021 agaacagaag tagccatgct gaaaaaaatc aatgatgcca aagagtacgg cattgctgag
     1081 atccacgcaa tagttgaaac tggagatcca agaaatttga ttgccaatgt catccctgga
     1141 caagaaaata ttgatttgat cgttatggga gcgacaggaa aaggtgctat acaacaagca
     1201 ttagttggat cgaccgcttc ttatgtcgta tcacatgccc cttgcagtgt gttggtcgtg
     1261 aagtaatgag ataagggagg aatagaaaat ggttcgtcct caagaagtcc gtgcgccaaa
     1321 agaaaaaata gaaattttag caattattga agacggcaca caaacaaaga agggatactc
     1381 gatcgcttta gtaaagtgga aaggaaaaaa aggtgttgct ctgagatggg atggagataa
     1441 ccaacaagat aaaggattcc ctgtcacagc taacggttat catccagcgt ggtttgttct
     1501 gcctgataaa tttatggaat tatacagcta tgattatgca agagccgcag catcaatcaa
     1561 agcattagat aaattaacag aagagcagaa tgaaatggag gaaaaataaa aaagacgaac
     1621 caatcagcct ttagaaaact gattagttcg aggagtaaaa atgaaaaaag gttatttggg
     1681 atgaaactag aataatgcgt ataacttaaa taaaacttaa agcaataaaa tggcaagagg
     1741 gtgaggcaaa tggaagcagg aaaagtatta caggaagtaa aagcactatg tgcagataaa
     1801 aaatatgaag aagcgaaaag cctgatcgaa aagaacaaag aaaatttgga agaaaagtac
     1861 ccgatcgcac aagaatttat agaattaaaa caaggaacga tccttcaccg attcaaacat
     1921 ttttttggta taacagaata gaaaaaacga ttaaagatcg gcagaaattt gctgcatctt
     1981 taatcgtttt ctttttaaac atgcgaggga ttcaattctg attcttatat aaaaaataga
     2041 tccaaataat a
//
LOCUS       DACDHJ010000267         2043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDHJ010000267 DACDHJ010000000
VERSION     DACDHJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.271"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..467
                     /locus_tag="IV223_002498"
     CDS             <1..467
                     /locus_tag="IV223_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712742.1"
                     /translation="CFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYL
                     MTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSS
                     FLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(543..878)
                     /locus_tag="IV223_002499"
     CDS             complement(543..878)
                     /locus_tag="IV223_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712743.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            1005..1220
                     /locus_tag="IV223_002500"
     CDS             1005..1220
                     /locus_tag="IV223_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712744.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            1257..1619
                     /locus_tag="IV223_002501"
     CDS             1257..1619
                     /locus_tag="IV223_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8712745.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            1665..1991
                     /locus_tag="IV223_002502"
     CDS             1665..1991
                     /locus_tag="IV223_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712746.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          719 a          287 c          423 g          614 t
ORIGIN      
        1 tatgttttaa cgaaaaagag gctaaaagag tatttcctca aaattcccga tcagaactca
       61 gcaaggaaaa acaattgatt ttagatgaag cacatgaagt tttacagggc ttatcgtggc
      121 aagggacata tctaatgaca gagaaagaac ttattaacac tgtttgtgaa cgcctacgta
      181 atgttgggga taacagcgtg aaaaatgctt taataaaaaa cagaggctac tttgttaaac
      241 aaggattcac tcttgaacga gcttctaagg atattaagaa actatacaat gtgaaagcaa
      301 gtagtagttt tttaatatat ttagcgaaag gggcagggat agatgaaaca ataaaagaat
      361 tgatattatc aggagctggg atagttcctc tgtatcaatt tacaagtgta acaagagatg
      421 atggacggag agtaaggaag agaaaagacc caattgtatt aaactagaca ggttatccat
      481 agctcttaat cttatttcat ctcaatacac ccaccgttaa agttcggtgg gttatttatt
      541 tttcaatctt tcaatagctg attgcgttca ttttcttgcc tttttaatag cttcatatag
      601 tcaattgttc gaactgcatc ttcagtgcta agagaacgat agagagccaa aaactcttcc
      661 tcataatcta cgacaacatt gtctagaaaa tggcttacag gggttcctag ggctctagaa
      721 agagctgtta agctatcgag gttaggctct gttacagaat tttcccatct tgctactgcc
      781 tgttgtgtag agaagatttt ttcagctaat tctttttgtg tccacccgag agcttttcgc
      841 ttttctctaa tgacctttgc atattgtttc tgtttcatta atacaccccc atctcagtat
      901 agtacaacaa gttgttgtat ttgtaatggt atttgaagtt ttttatattt tatgagttga
      961 aatacaccat attgttgtat ataataaaac aggaggggag aatcatgaaa aaagtaagta
     1021 atctcagccg atacagaaaa gaaaaaggac tttctcaaac ggagttagca aaaaaaatga
     1081 atgttacaca gcaatgtgta agttcgtggc aaacagggag aacgattcct aaaccctatc
     1141 aaatgaagat gctttctgag atattaagtg taccaatgaa tgagctgttt tctgatgtat
     1201 ttaataaagt aaacagttaa ctaataagca attgaatgat tttaggagga gaagacatgt
     1261 attgtagaaa atgtggcaaa gaaataacag atgaaagtaa gtattgccag ttttgtggag
     1321 cactacaaga aacagatgag acaaccggaa cagacacagt aaagaaaagt actgagcaga
     1381 caattaatga aatgaaggaa caaatcgtac atggacaaga gacagggaag ctgtatttga
     1441 ttattggttg gatttctatg gtagtatcgt tagcatttat tcctgttgta tttggtgctg
     1501 ttgctgttat tatgggctac ttatatcgag aaaaagatga gaaaatgggt acgattttga
     1561 tgatagcagg ggtagctgga gcaatctttg gtgtactttt agggatgtca atgtactgat
     1621 aagagggata agaacaaatt attttggaga taggagataa aaagttgaat aaagtaaata
     1681 tgaacaagaa gtttgtcatt ataggtattg ttgtcttggc tattataggt attatttttt
     1741 caaaaatgac aggtacatct ggattagaga agtcatcaaa accattggta acacagattt
     1801 tacaagaaca gtacaactta aatcgatcat gtgatgatgt gacgattact catgaaaatg
     1861 gagataacac ataccgggct aaagctatat tagacaatgg aagtgctatc aacataaata
     1921 ttgagtacta tccaaagaaa gaccgtatat atgtggagat accatatgct gaggtgttaa
     1981 tgttaaattg atttaaatgt attatgcata ccgtccaaac aggaactccc aaaggtgtaa
     2041 att
//
LOCUS       DACDHJ010000268         2040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDHJ010000268 DACDHJ010000000
VERSION     DACDHJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.272"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..1745
                     /locus_tag="IV223_002503"
     CDS             132..1745
                     /locus_tag="IV223_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8712747.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          649 a          358 c          416 g          617 t
ORIGIN      
        1 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
       61 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
      121 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      181 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      241 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      301 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      361 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      421 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      481 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      541 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      601 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      661 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      721 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      781 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      841 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      901 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      961 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
     1021 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1081 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1141 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1201 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1261 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1321 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1381 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1441 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1501 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1561 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1621 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1681 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1741 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1801 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
     1861 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
     1921 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
     1981 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
//
LOCUS       DACDHJ010000269         2037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDHJ010000269 DACDHJ010000000
VERSION     DACDHJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.273"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IV223_002504"
     CDS             complement(<1..914)
                     /locus_tag="IV223_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8712748.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IV223_002505"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IV223_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8712749.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          677 a          368 c          396 g          596 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttattta
//
LOCUS       DACDHJ010000270         2019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDHJ010000270 DACDHJ010000000
VERSION     DACDHJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.274"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IV223_002506"
     CDS             100..570
                     /locus_tag="IV223_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8712750.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IV223_002507"
     CDS             792..1895
                     /locus_tag="IV223_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8712751.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DACDHJ010000271         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDHJ010000271 DACDHJ010000000
VERSION     DACDHJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.275"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IV223_002508"
     CDS             <1..378
                     /locus_tag="IV223_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8712752.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IV223_002509"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IV223_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8712753.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IV223_002510"
     CDS             1859..>2003
                     /locus_tag="IV223_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8712754.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACDHJ010000272         1979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDHJ010000272 DACDHJ010000000
VERSION     DACDHJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.276"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..881
                     /locus_tag="IV223_002511"
     CDS             54..881
                     /locus_tag="IV223_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712755.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1154..1924)
                     /locus_tag="IV223_002512"
     CDS             complement(1154..1924)
                     /locus_tag="IV223_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8712756.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGKMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
BASE COUNT          654 a          300 c          356 g          669 t
ORIGIN      
        1 tttttgtgta tgatataaag gagaaataat tttttaaagg gaaggaaatt gttttgatta
       61 aagtagatgg agcattacta aaagaatacc gaaagaaaaa ggggttaaca caagtacaat
      121 tagcagaagg catttgtgaa caagcaactg tcagcaatat cgaaaacaag aatatgtcgc
      181 caggcatcaa tatcttaagt gcgctttgca aaaagctgaa tgtcgagatc tccgatatat
      241 tagtagaaac agaagaagac aaaatcagga aaaaaataaa tgagattcaa tcattgaatt
      301 ctgtatcaaa ataccaagaa tcttataaaa ttttacaaac gatcgatcag gaaaaagtaa
      361 gtaattctga tcttttaaat caaattttat tttatcaagg actgcttgat catctttatg
      421 cgaaaaaacc agatgcagca ttattttatt ttaatcaagt gttggaacaa acgaccgaaa
      481 cagatatcca tcacttacaa gcagctgcaa acgtggcaat gatctacttg tcaaaaggag
      541 aattagattt tgcgaaagtt tatgtggagc gtacacttaa aatattgagt gagactgatt
      601 ttgataatct gatggtctgc attgtatatt atgatatcgc cacctattac cgtaaaatcg
      661 aggattatga taaagcaatc caattatgtg agaagggtat tgagtataat aagaaacata
      721 aatcgactta tgcattggaa tacttgcttt atgaaattgc ttcttgccat aagcaattag
      781 gagaagatga ttatttagaa agatatatgg acgcaaaaaa aatagcgcgt ttcaacggaa
      841 atgattacgc ggtaaaggtg attgaaaacg acctgaaata aagtggttgt gaaatcagtg
      901 ttctgatttg aataccgttt attcgaaaaa aggaggtgtg acttttgtca tacctccttt
      961 tttcgatatt gtttgattcc tatttaggca acatataacg gttataaaag aagcaataga
     1021 ttatgatggt atcgattgga tggatacatc ttgggattcc tttatcaatt tgagcgtagc
     1081 gtgtttttca tttatcgata aatttgttat tcattttttt gataatccta tttacatatt
     1141 tacgtcctgg ttcttaaaat gaatctactt gaatggtttt tatccccgta tttctgatct
     1201 cctctaatac tttttcagaa caaaatttat cagtgatcaa ataatcaaag actgaaatat
     1261 cagcaatctt gaactttgat gtcagtccga tttttcgatg atcagcgact aaaatcttac
     1321 atttgattgt cttgttgacc atcatttcgt tgatcgttga ttcatttaat atctttgttg
     1381 tgaccccatg tactacatcg actccgctac agccaatgat acaaatgtct gcattgactg
     1441 aagaaatcgt atttaatgcg atgtcgccca ctagtgcttc ttttggaaag cgcatctttc
     1501 caccagttaa aatataacta caattatctt tgtgcggaga gtaattgatt ttcacgttgt
     1561 ttgtaatgac tgtcaaatgg ttagattgaa gaaaatccag tgttttcaac gcagtcgagc
     1621 tagaattgat aaacagcgta tcataatcgt taatgtattt actagcttct ctggcaatcg
     1681 cttgtttaat cctttcagga ccagattgat caaaatcagt ctggtttttg taattataag
     1741 aacaataacc gtaattgcgt atgatctcgt ttttttcttc taataaattt aaatctcttc
     1801 gtatcgtact aatagataca tcaagggatt cagctaattc gaaagttgat agttgttcag
     1861 tctgctgaag cagttccaaa atttcttttt gacgtttttc aatattgaca agtgagtttt
     1921 tcatgacttt tgcctcctga gggacatctt gatccatgga tttatttgac tagttgttc
//
LOCUS       DACDHJ010000273         1978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDHJ010000273 DACDHJ010000000
VERSION     DACDHJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.277"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(362..679)
                     /locus_tag="IV223_002513"
     CDS             complement(362..679)
                     /locus_tag="IV223_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8712757.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(680..934)
                     /locus_tag="IV223_002514"
     CDS             complement(680..934)
                     /locus_tag="IV223_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8712758.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1194..1412)
                     /locus_tag="IV223_002515"
                     /pseudo
     CDS             complement(1194..1412)
                     /locus_tag="IV223_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          629 a          367 c          304 g          678 t
ORIGIN      
        1 taaatactac cttatttagg tagtatttat taaaagttac gtcaattttg attaaaaata
       61 tactacttga acctctcatg agtaaagtca cgaaattttt gggaatagag cgtacttgat
      121 atcttatttt ttcactaata gcagtgtctc ttattcacca agcgatcccc gtagttccta
      181 cggttcttaa tttttctcta gactaatgat tgtatcttta gaccttcggc cataacatgg
      241 agcaaaataa aaaggtacta agtcttatat atcaatcttc gagtaaccga cggttaacaa
      301 ccatatcgag cggcattaat cacccacctg tagaggatga gcgacttttg cctaattatt
      361 gttaaaagcc tagtttcatt tgtttagatt ggaaaatacg tcctacaagt atctcttttt
      421 tttcatggtc gatccgataa aataaggcat agcttttgcc gatcacgatc cgatatgttg
      481 ggatatccat tccatagatt tcggaaactt cctcatgcat agttggaaaa tttttggtta
      541 gttctaaagc cttctggatc gcacctacaa atctttttat gctggctgga gaaagagcaa
      601 gctcattttc ccattcttct attatcgttt caaggctggt ctggaaactt ttagaataaa
      661 ctagcgtgta ctctttcatc tattcactcc agccgtattt tccgaaagct tttaccattt
      721 caatttctga aactgttaaa cctttctcaa agtcttggaa actttcttca atcaatttgc
      781 gatcgtgttc ttttgctatt tgagtagtat ctaatatgaa taagttgcct tcttgtttgg
      841 cttggatagt gtctccgctt tttaatccaa gagagcttgg aaggatcact cccacgctat
      901 tgccgatctt tctaaccttt agttcttttc ccatgttatc acacttcttt tagtttttga
      961 taaataaatt ataacatgtt ataacgacaa ttgccaacgg tgtttcttaa gggattccac
     1021 tccatattat tattggacaa aataagccac ttagaacgct gaaaacatat acaaacgata
     1081 tcaaaatacg agtgaactac tcaccataca cttccgttaa catccattta agtaggtatt
     1141 tttattatat atagatttaa catatgtcgg tcggttgatt gatatagcta aagttataac
     1201 tcaaccgaag ccgattcatc tacacgaata aagttgcgag tgttctcggc taattcataa
     1261 aaattgttat gtgttccata tagcaatact ggaataactt tattttcttg aatctcataa
     1321 gcaactctat acacggtgac cgcatactta aatccattag accaattacc tgataaacct
     1381 ccagtttttt gctcttactt aaatggatca atagcaattt catcacaaac ctatagagga
     1441 tgagtgactt ctgcctaatt acgttaaaaa atattgaaag taactctatg tagcaaaaaa
     1501 tatctagaat ttcatttcct caaaactgat cgatattgta tctaaaaata tatactacct
     1561 aaaataggta gtatatatta aaatatgtgc ttttgttaga aaaatcaata cataaattat
     1621 catatctgta tatgtcattt aggtatttca gttatgttat aatagttttg ctgtctgcta
     1681 ccccggccta atctggcaag gggaggagga gatataatga aatacctttt tgattttgtt
     1741 tttgcaatca agccatttgc agaggtaact gcacactaca tctgcaagtg gcttgattgc
     1801 aaaattgcca aaaaagacag taagcctacc agttaattct ataatataga aacgtaaaaa
     1861 aaaaccccat cagcctacac gctgatgggg tttgatgata taatgaaata cctctttgtt
     1921 actcataatt ataccataat tagtcacgaa tgcaaccaat agaaaaaact acttagaa
//
LOCUS       DACDHJ010000274         1977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDHJ010000274 DACDHJ010000000
VERSION     DACDHJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.278"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..>1977
                     /locus_tag="IV223_002516"
     CDS             11..>1977
                     /locus_tag="IV223_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP8712759.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRL"
BASE COUNT          714 a          299 c          398 g          566 t
ORIGIN      
        1 gaggaagaaa atgaagaaaa gtacagtatt ttataaaata tttttaccga tgtttttatt
       61 aggagccgta cttgtcatag gatttagttt attcatttac cagaatacct atgaatcgat
      121 cgaatcgagt tatttaacag acaaaaagaa tttgctcagg caagttaaaa ctaacgttga
      181 atggaaaatt agaacgattg aatattcgtt ttctacgtat ggttcgacca aaaatttctc
      241 ggacatattc aagcatccgc tgacatatac agattactcc acttattctg aaatcaggaa
      301 agaactaaat tttatagaaa cgattgttat ggatgataat aattatgatt tagtcagttt
      361 ggctggtaaa tggggagtaa tcaatggatc gttgagtcaa ctgaccgaag aggaaattga
      421 aaagtatcaa cagaagtaca taaataacaa aaataattta ttttggcaaa agaaaaacaa
      481 aggaatcgaa atggtgattt ctttgccaat gtttgaaaaa gaaaaatatg cattgggaat
      541 tgctacgata gagaaacata caatagaaca agttgttggt gaaagtggag aagacatact
      601 ttctattgaa aacgaagttg gcaacctttt tactactcag acagtaaaaa ataaagagca
      661 accaactgta ttaacggacg tggatctaga ttacaatcag ctgaaagtga taagagaaaa
      721 agataaaacg tatatagtac tgaaatcaga ttataataat tggatttaca gattagaagt
      781 tgactctagt gcaattggcg caatgatccg caatctgaga atcgggctag ttgcagtgtc
      841 tttaacactg gtaggactca ttggtcttct ttcctactta ttttcggaca gatttgcccg
      901 tccgatttct caaatacagg aacgtctgaa tcttaaatca aaaggattca gtgggaaaga
      961 actagatttg gtcgctcaat cagtcagtcg gatcatagga gaaaaagaag cgttaagtgc
     1021 acacttagtt acccaaaagc cacagctgga aacgctattt gtacttagtt tatttagaaa
     1081 tcgagtagaa aggcgagaac tagatcagcg gttgcagcaa tttggttata gttctcaaaa
     1141 tgactgctat tatacagctt tggttcaaat cgatctgtta gaagatagtc acggtgggga
     1201 gcgtgatttg ctacttttag cgatcaacaa catgatcacg gaaattgtgc caaaggaaaa
     1261 tcgaatgctg ccgatcgttt tgaatgaaga aatgcaagcc actatttatc gaatggataa
     1321 agatgatgca gaagcaagca aaaaagtcat ggagtattgc cgactcattc aaaaacgtgt
     1381 aaaagaatac ttgaatttaa ctatcagtat aggcatcagt aaccggttcg aaacgctgaa
     1441 tgaaagcaag caatcggttg atcgggcaaa agaagccctg tattatcgtg taaatactgg
     1501 tccaagttca attatttttt atcaggaaat cgttcctgct gctcatgaaa aaacacttat
     1561 tagatatccg atagaagaac aaaatcgatt gtttgaagcc attcgttctg gaaatcagga
     1621 agtgaagaat ttagtacatg aattgattga tgcattgttt gcacaaaata aaaatccttt
     1681 gagtcgggaa gtagtttcgg tccgcttgat caatgaactc gttcaattag gacagttatt
     1741 gggagtagat tcaaaaaact ttgataacat gaaacaaatt tatgtaaaag cactgaacaa
     1801 ttatcatccc aaagaactag aaaaacttgt tttagaacaa ttagttctgc caatcacaga
     1861 atgctctcaa gcaatcactg atcatgaatt taaaaatttg tcggagaata ttatgcatat
     1921 cgtctacaca gaatatgatc gtgatctgtc tttagatgtg atcgctgata ggttgca
//
LOCUS       DACDHJ010000275         1965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDHJ010000275 DACDHJ010000000
VERSION     DACDHJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.279"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..610
                     /locus_tag="IV223_002517"
     CDS             <1..610
                     /locus_tag="IV223_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8712760.1"
                     /translation="LLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRG
                     GKTSRLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENE
                     MLRVLDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALEL
                     IQETSFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            906..1850
                     /locus_tag="IV223_002518"
     CDS             906..1850
                     /locus_tag="IV223_002518"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8712761.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          688 a          294 c          374 g          609 t
ORIGIN      
        1 cttattaaaa gaagaaacct tatctacaga gatattgaat catttggcaa gcacaaataa
       61 tctactttct tcttttttga taaatgcatc aaaagaaaaa ggggaacata cacaattcat
      121 tcattttcgc ggcggaaaaa caagtcgttt gacgcatttg attgaaaata tgctgttgat
      181 ttattttgat acatacagtc ataagatccg ttcgttaaat ctctatacat cactattact
      241 gatcgaattg actcatttat tagaacaaca aagcttttca tcagcagatg gatgcgcaga
      301 aaatgagatg cttagagtat tagattatat cgataagcat tttacttctt gtacgttaaa
      361 agaaacagca gctcattttg gctacaatcc ggtctatctt agtaataaat tgaaaatgta
      421 ttttgatgct tcctttcaag aactggttct aaagaaaaaa ttcgactttg cgttggaact
      481 gattcaagaa acgagttttt cgttggaaga aatcgctaat cgtatcggat ataaacaaac
      541 gacttcttta ttcaaattat tcaagaagta ttcaacatgt actcccaatg aatggagaaa
      601 taaacattaa gaagaaaatc tttcactaaa gcatatgaag gaaaattgtt ttaggtgcga
      661 ttgctgtctt attatatcgt tgcagcgaat ttttttggat ttattttcag ttctaaactt
      721 ttggttgtat tatcagggga tataggtgta aacatttgtc atttatctgt tatgcgtatt
      781 caggaattcg tttccatcaa aaattataat aacaactatt ataattaaca tgttgataaa
      841 atgaataaac aagactatac ttttcatgaa agtaacaaat agttatgaaa aggagctgtt
      901 tattcatgaa aaaaacaagt cgtaaagtag taatcgtcgg tacaggtttt gttggaacaa
      961 gtatcgctta tgcaatgatc aaccaaggaa tctctaatga attagtattg atcgatgtga
     1021 atcaagaaaa agcagaaggg gaagcattgg accttttaga tggtatggct tggggcgatg
     1081 aaaatgtggc tgtatggtca ggtggctatg aagaatgtaa agatgctgat atcgttgtta
     1141 tcacagctgg tatcaaccaa aaacctggtc aatctcgttt agatttagtt aaaacaaatg
     1201 cgtcaatcat gcgccaaatc gtaaaagaaa tcatgggatc aggattcgac ggtattatcg
     1261 ttgttgcttc aaacccagta gatattttga catatatcgc ttggaacgaa tctggtctgc
     1321 caacatcacg tgttatcggt actggaacaa cattggatac aactcgtttc cgtaaagaaa
     1381 tcgcattgaa attaaaagtt gacccacgca gcgtccacgg ctatatctta ggtgaacatg
     1441 gtgattcaga agttgctgca tggtcacata caactgtcgg tggtaaacca gtgtttgaaa
     1501 ttgtagaaaa agaccatcgt attgcccaag atgaattaga tgtgattgct gataaagtcc
     1561 gtaatgctgc ttatgagatc attgatcgta aaaaagcaac ttactatggt attggtatga
     1621 gtactgcacg tatcgtaaaa gctatcttga ataacgaaca agctgtattg cctgtatctg
     1681 cttatttaac tggtgaatac gacgaaaaag atatctttac aggtgtgcca tcaatcgttg
     1741 acgaaaatgg tgttcgtgaa gttgttgaat tatcaatcaa cgaagaagaa aaagcaatgt
     1801 tcagcaaatc aacaagtgct ttaagagaag tattgaacac tgtattataa gaaacaatga
     1861 atatgaacta aaaatcctaa aactgcttaa cagctgaaat agctgttaag cagtttttta
     1921 ttgatatcta ataggtaagc aaataaacag aatggttaaa attaa
//
LOCUS       DACDHJ010000276         1911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDHJ010000276 DACDHJ010000000
VERSION     DACDHJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.280"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IV223_002519"
     CDS             5..1150
                     /locus_tag="IV223_002519"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8712762.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IV223_002520"
     CDS             1321..1779
                     /locus_tag="IV223_002520"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8712763.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          622 a          361 c          378 g          550 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat t
//
LOCUS       DACDHJ010000277         1902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDHJ010000277 DACDHJ010000000
VERSION     DACDHJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.281"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            215..781
                     /gene="amaP"
                     /locus_tag="IV223_002521"
     CDS             215..781
                     /gene="amaP"
                     /locus_tag="IV223_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8712764.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            794..982
                     /locus_tag="IV223_002522"
     CDS             794..982
                     /locus_tag="IV223_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8712765.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            995..1552
                     /locus_tag="IV223_002523"
     CDS             995..1552
                     /locus_tag="IV223_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8712766.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1584..1826
                     /locus_tag="IV223_002524"
     CDS             1584..1826
                     /locus_tag="IV223_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8712767.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          691 a          299 c          398 g          514 t
ORIGIN      
        1 taatatcctt taagtatagc cagcaatcaa aagtaaatag agaataagta agcaattgtt
       61 atttcagtat cagactggca ataaaacgcc gttatgatcc agcaatgttt ggtagcgctt
      121 tatttactga tgctatgcta actgttcgct atctatactg tgattgtcgg tagagttatt
      181 tacagggtaa aaataagttt ataggaggtg gaaaatgaat aaggggttaa aaataatcgg
      241 tgttatcatg agtttactgc tactttcagt atttctagcg attgcgttga tcaatgcgcc
      301 gtatattatg cctgaacaat tggaacgatt tcgcttcttt actttaacga attactacat
      361 gcaacaatat attttctggg cagctgtagt atttgccgtt cttgctatta tcatcttgct
      421 agtcgttctt ttctatccaa agtcaagagg aactttcgtt atgaaacggg aagatggcaa
      481 actaacaatc gacaaaaaag cgatcgaagg gttggtacgt tcccatttac acgaagaaga
      541 gttcattcat tcacctaaag tacggatacg ttcaacgaaa aatcgaatcc atatccatgt
      601 caacggagac ttgaaacgaa cttcttcatt agtagggaaa acaggtgtat tgatgcaaga
      661 tatcgaagaa gaagtaacta aagtactcgg aacaaaagaa acggtcaaag tggcagtcac
      721 atacagcggt tatgaagaac aagaagatac aagagactac aagcactctc gagtggaata
      781 ggagggccaa ataatgaaag aactattttc agcatacaag ctccctatta tctttggggg
      841 tcttggacta ctgctagctg ttttatggtt aacgattgga tttttcaaga cattgttgat
      901 cataatcatg acagcaattg gcgcaggaat cggtttttat ttgaaacagt caggaatagt
      961 tgatcaattt tttaaatcat aggaggaaac aaagatggaa aacaaaaaca tgaatacaac
     1021 aaatggatca gttttggcaa atgaaaaagg agaaaaccgc atgatgaatg gtgaattgac
     1081 ttatgaagac aaagtcatcc aaaaaatcat cggttatgca ttgaaagatg tagatggtct
     1141 attgacatta gatggcggat ttttctcaaa tatggctgaa aaattagtca acacagataa
     1201 cgtaacagca ggtatcaata cagaagtagg taagaaacaa gtagctgtgg atctagatgt
     1261 gatcgtagaa tacggtaaag acatcgaaga tatttacaac caaatcaaag aattgatcag
     1321 tactgaagta aacaatatga cacatttaga tgttatcgaa gtgaatgtga atgttgcaga
     1381 catcaaaaca caagaagaat accaaaaaga tcaagaaaca gtacaagaca aagtaacaga
     1441 agcagcaaaa tccacaggac aatttgcttc acgccaaaca gataaagcaa aatctgctgt
     1501 aggcaaaggt gcacaaaaag tcaaagaaaa taacgaacca cgtgtacaat aagaagtaag
     1561 aagtcagtta ggaggaattt aaaatgggat ttatttgggc attaatcgtc ggcggtatca
     1621 ttggagcaat tgcaggagca atcacgaata aaggatcatc aatggggatc atcgcaaaca
     1681 tcattgctgg tttagtaggt tcagcaattg gacaagcttt actaggaact tggggaccaa
     1741 caattgcagg aatggctatc attccatcat tgatcggtgc aatcatttta gtattagtcg
     1801 tttcattctt tgcacgtaaa ttataagcaa attgaagaaa aacttatgtt gaaatgaaga
     1861 acaaatttga gaaccgtagc gacactcgct gcggttcttt tt
//
LOCUS       DACDHJ010000278         1883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDHJ010000278 DACDHJ010000000
VERSION     DACDHJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.282"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..448
                     /gene="ssb"
                     /locus_tag="IV223_002525"
     CDS             29..448
                     /gene="ssb"
                     /locus_tag="IV223_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8712768.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            525..821
                     /locus_tag="IV223_002526"
     CDS             525..821
                     /locus_tag="IV223_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712769.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            836..1267
                     /locus_tag="IV223_002527"
     CDS             836..1267
                     /locus_tag="IV223_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712770.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            1449..1754
                     /locus_tag="IV223_002528"
     CDS             1449..1754
                     /locus_tag="IV223_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712771.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT          714 a          259 c          387 g          523 t
ORIGIN      
        1 ataaatagaa gaatggagag aaaaaatcat gatgaattca gtgaatttag taggacggtt
       61 aacaaaagaa gtggatttaa aatacacacc aaaaggcaat gcgacaggca catttatttt
      121 agctgttaat cgaaattata cgaatgcaaa cggcgagcgt gaagcagatt atatccgctg
      181 tgtcatttgg cggaaagctg cggaaacttt agcaaaatat actggaaaag gaagtttgat
      241 tggcattaat ggacgtcttc aaacgaggag ctatcagaat cagcaaggac agaccatgta
      301 tgttacggaa gttctagtga cagatttcta tttgttagag agtaaagaaa tcaatgagca
      361 acggtcaaaa tcaatggcga ataatcaaaa taattcttca attccaggaa atgagattca
      421 aatttcagat agttatttgc ccttttaagt gaaaaataat tttgttctat acctgatttc
      481 ttcaggtata gaacaaaaaa taaatagaga tgaggcgatt ttttatgaaa atcattgaca
      541 gttacgatca acgaaccatg caatatttgg gctcgtttga ttatacagat gaaaatatta
      601 ttaattatgt tgcaagttta cctccttatc aaagcgttcg cttggtagat tttgcaagtg
      661 atgaattggt attaacaacg atgggcaact ttttagatta tgtcggagac aaaaaatggt
      721 tggaaaaaat tttgtctaaa ttgattgcga aacaaacagg agaagaaccc ataaatgcga
      781 ttcaattgtt caatcgatat gaacaagaaa acaatgatta aagaggagag aaaaaatgaa
      841 aagattaaaa cgaagtattt ttattgtgct agcattgatt gattttgttg ggacaggttg
      901 tgaagcaatt gacgttcaaa aagctccctt atcggaaata aaacaagcaa taacaaaaca
      961 agatgaagaa caaccgctaa atacacaagt agaaatagtg aatcaaaata aaccgagttt
     1021 ttctgaagaa gatttttcgt tagaaaatgg aaccaggcaa tttttttcag atttggatcg
     1081 tttaaatcgg gtaggtgtag caagtgcact tttgcataaa agcaggatgc caacagaaga
     1141 acgagaccga ttatacatta agccaactgg ctggaaacaa aagaaaatga agaatggcga
     1201 ttatctttat aatcgttgtc atttgattgg ctatcaacgt gcgacaagtt tccccataag
     1261 tatgtaatac gaaacgggag gattaatctt aagattaatc acaactaacc tatttgacag
     1321 ttggaatgaa agctatcatg tctgctgaat caactggtct gatactccta catgcaataa
     1381 agtctactag gaagttattg tatgaagcta ggtaaaggcg gcagaacaat ttctaagtgt
     1441 ggaatcatat gaagttggat atgccgggac gctataaatt actcagggtg agactgtttt
     1501 taattaaact gacgaacttg cgaatgtacg ggtctataac cgacttatat tcgaaagata
     1561 taagattgcg aatgcaaaga acattatcga aaagtaagaa tgttggtcat gaaattcgag
     1621 atggaagact agcggtgtat ctgtcaagag ttggtaaaac atgtttgatt gattcagatg
     1681 agagtgggaa tgcgacaaaa cgttttacag aagaaataga gcaactagca gaactgaata
     1741 tacgatattt ttaaatactt tagcggagaa taaaaaaatc ctctacagaa aactacggag
     1801 ggcaacatgt gctattgttt caacaataca tttttaattt ttaccataac aaaaaagtac
     1861 ttgaaaaaaa acagaaatat gtt
//
LOCUS       DACDHJ010000279         1874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDHJ010000279 DACDHJ010000000
VERSION     DACDHJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.283"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..551
                     /locus_tag="IV223_002529"
     CDS             69..551
                     /locus_tag="IV223_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712772.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            748..1638
                     /gene="pstS"
                     /locus_tag="IV223_002530"
     CDS             748..1638
                     /gene="pstS"
                     /locus_tag="IV223_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8712773.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          616 a          319 c          389 g          550 t
ORIGIN      
        1 ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag tagtttgggg
       61 gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt tggttttttc
      121 ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg gagatattag
      181 gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg attgttttct
      241 atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac tacaattggc
      301 agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg ctgcctcgct
      361 tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggatttagct tattcctcca
      421 tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt attcttgcaa
      481 cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta tctataaaaa
      541 ataactattg atggtggatc atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt
      601 ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat gatttttaca
      661 taacttaact atatttttac agcaaccata cgagggattt acagtcgact gataaacttt
      721 agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct aggtatgatg
      781 ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc tgccacacaa
      841 aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc gacagcatta
      901 cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc
      961 tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg agcagttact
     1021 atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc aaaattagtg
     1081 gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac agatgtcaaa
     1141 gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa ctggaaggaa
     1201 gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc
     1261 gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca agaacaagat
     1321 tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag ctatttagct
     1381 ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga accaaaagaa
     1441 gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta cacgaacgga
     1501 aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga aatccaagaa
     1561 ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga acgtgatcac
     1621 gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac tattaaggaa
     1681 aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa tagttcctca
     1741 tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttattcgaa
     1801 aaaaagagct acgacataga actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg
     1861 gtgaagacaa attt
//
LOCUS       DACDHJ010000280         1871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDHJ010000280 DACDHJ010000000
VERSION     DACDHJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.284"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..1011
                     /locus_tag="IV223_002531"
     CDS             7..1011
                     /locus_tag="IV223_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712774.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1012..>1871
                     /locus_tag="IV223_002532"
     CDS             1012..>1871
                     /locus_tag="IV223_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8712775.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKE"
BASE COUNT          636 a          365 c          404 g          466 t
ORIGIN      
        1 attcgattgt ccgataacca attattagaa gttcagaacc tacatacagg attccgactc
       61 aaagatgact attacgatgc agtagatgac gtttcattta ctttagacaa aaacgagatt
      121 ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct tggctactac gattatggga
      181 ttacttgatc ctaataacac aaaaattaca ggtgagattt tatataacga tctgaattta
      241 atcgacctga acgaaacgct ctataataaa atccgcggaa atgatatcgg aatgattttc
      301 caagatccgt tgtctgcatt aaatcctctc atgagaatcg aagaccagat caaagaagga
      361 ctttcctatc acacaaagat gaatgcagaa cagcgtcaag ctcgtgcact agaattgcta
      421 gaacaagtag ggattcctaa tcctgcgaga gttggacgcc aatatcccca tgaactatca
      481 ggcggtatga gacaacgtgt catcatcgca atcgcaatcg cctgcaaacc tccaatcgtc
      541 attgcagatg aaccgacgac agccctagat gtaaccatcc aagcacagat tcttgattta
      601 ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct tgatcactca tgacttagga
      661 gtcgtagcag aaatggctga tcgagtggca gtgatgtatg gcggacagtt cgttgaagta
      721 gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt atacacgctc tttactgaac
      781 tctataccac aagaaggaaa tcatgatgca gaactccatg tgatcgaagg tgtcgttcct
      841 tctctgaaaa acatgcctcg taaaggttgt cggtttgcac cacgtattcc atggatacca
      901 gcaagcgatc atgaagaaga gcctatcctt catgaagtcg caccgaacca cttggtacgt
      961 tgctcctgct ataagcattt ccactttaga gacgaaaaag gagaggtgta gatgacagaa
     1021 ttaatcacaa tcaaagacct gaaagttcat taccctattc gcagtggatt cttcaatcga
     1081 gtaacggatc atgtatatgc tgtagatggt gtggatttta ttatcgaaaa aggaaagaca
     1141 tatggtttag tgggagagtc tggctctgga aaatctacta cgggaaaagc cgttgtggga
     1201 ttagaaaagg taacagccgg acaaatcatg tatgaaggaa aagatgtaac gaagcgaagc
     1261 aaccgcaaaa aaatgggtta taacaaggat gtgcagatga tcttccaaga ttcaatgtcc
     1321 agtttgaatc ctaaaaagcg agtgttggat atcattgctg aacccatccg aaattttgaa
     1381 cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac tgctagatat cgttgggatg
     1441 ccggaagatg cgctatataa atatcctcat gaattttcag gcggacaacg tcaacgtcta
     1501 ggagtagccc gtgctgttgc taccaatcca aaactgatcg tagccgatga acctgtatct
     1561 gctctggatc tatctgttca agcgcaagta ttgaatttca tgaaacgaat ccagcaagaa
     1621 tacggcctca gctatttatt catttcacat gatttaggtg tagtaaaaca catgtgcgac
     1681 aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg gtacaagaga agacatttat
     1741 aatgacccgc gacatatcta tacgaaacgt ctgctttcag ctattccgca aatcgatgta
     1801 gaaaataggg aaagccacaa agaaaatcga cgaagagtag aacaagagta tatccaaaac
     1861 caaaaagaat a
//
LOCUS       DACDHJ010000281         1832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDHJ010000281 DACDHJ010000000
VERSION     DACDHJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.285"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..489
                     /locus_tag="IV223_002533"
     CDS             <1..489
                     /locus_tag="IV223_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP8712776.1"
                     /translation="STFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQYI
                     EDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMHDQIEEEAKENI
                     FIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIRKRLLLEAEKAV
                     KE"
     gene            486..>1832
                     /locus_tag="IV223_002534"
     CDS             486..>1832
                     /locus_tag="IV223_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712777.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFG"
BASE COUNT          609 a          269 c          361 g          593 t
ORIGIN      
        1 tctacttttt tagaaggtga aacgattgta ctcgctcctt taaaacgaca agatttgaaa
       61 ggtcataaaa tacggccgta tcctattttt aatcatgggg aaacgaatct tccttatgct
      121 tatcaatata ttgaagattt caatccatcg tgggatatca atgatataga catacattgt
      181 acacatgcgg acttggatct taaggcttta caaattgatt acgattattt tctacctgat
      241 atctatgcac gtcgacttta tcacaatatg cacgatcaaa tagaagagga agctaaagaa
      301 aacattttta ttaaaatcgt tcaatcttta gacaactcaa cggcacaaaa gttacttatt
      361 caattttatg aagagaagca aatagatcgt ttgcaagaat accttgaaaa attgcgtcaa
      421 aatgaagaaa ttacagaggt tataaggaaa cgacttttgc tagaagctga aaaggcggtg
      481 aaagaatgat cattggagga atattttccg gcattgccga ctggtggtca ggcttatttg
      541 gggatgggga tgcagatccc aaaaccgtaa cgaaagtctt acaagattat ggtaattatc
      601 tgcagtatca agatgtattc agttggattt gtaatacttt tttatggggt ttgattaaag
      661 ggttatataa tctcaatact ttactagaaa aaacaattta tcaaagcttt gatttaaaag
      721 acttattgaa cgccgctggt gttaatgaac tgtttcaaag tttgattagt aaggtattgg
      781 ctcttttttg tgtcgtcaca ttaatttatt taggcttaaa attttttatg agtaaacatc
      841 caccaaaaat caaaaatatc ttggttaatc tatttatggc catgattttg atttttggtg
      901 gtagttcttt aatcgatgaa gggctaaata ttagtaaaaa cttttacgga gacgtgactg
      961 cagcaaataa agcagataaa actggttcac ctgcttttca aattattcaa aataatgtga
     1021 ttgatgtgag tacgttacta gaaaatgatc cgaacaaagt agaacacata ccaacagaca
     1081 aaagaaatgc tctcacggcg aaaaatttta aaacagcgaa tatcaatggg attattacac
     1141 ctgatcaagc taagaaaatg tctgaatcaa gtgatattag tacagtacaa aaagatcggt
     1201 taaaggcatt acagtatcga ctagagttag acgataatgg gaaagaaatc cctgtgcaaa
     1261 ttagtgatga aggtttagca aaatatgtgt atacttcagg ctatcgccga tacattagta
     1321 gtccaggtat tattttagct ggtgaatctt ctttgtcggt agcctattta tttatcttgt
     1381 ttacgattgt tacttgcatt attgagctgg tatttaaaaa attctattta gtgattgccg
     1441 cgtcaacgga ctttgagaca ggacaacgga tgaaaagtgc tatagaagat atttctcaat
     1501 ccttcttgtt attggcattt acgattttag aattacgaat ctatacatta atgctttcag
     1561 gtatcggtga tttgcatgcg gcaggaaaga tcaatggatt cttgtatgtt gtcgctttaa
     1621 ttgtattaac cattgcgcta ttcaaaggct ctcaatcagt tacgaaaatt ttcggcgttg
     1681 atacgagtct gaaaaacggc agcaattctc ttcttagcat gtttgcctta ggaaatgttg
     1741 cgaaaaatgt gggtagcggg gctaaaaacc ttgcaagtgc tggtaagagt ggtttaagca
     1801 atttaaatga tattcgtaaa aatggatttg gt
//
LOCUS       DACDHJ010000282         1816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDHJ010000282 DACDHJ010000000
VERSION     DACDHJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.286"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(372..869)
                     /locus_tag="IV223_002535"
     CDS             complement(372..869)
                     /locus_tag="IV223_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8712778.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(909..1718)
                     /locus_tag="IV223_002536"
     CDS             complement(909..1718)
                     /locus_tag="IV223_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8712779.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          521 a          341 c          315 g          639 t
ORIGIN      
        1 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
       61 acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg
      121 tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt
      181 ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt
      241 cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc
      301 acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa
      361 gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg
      421 gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga
      481 aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact
      541 acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa
      601 gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa
      661 agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt
      721 atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa tctttgagca
      781 atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata
      841 tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt
      901 tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt
      961 gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac
     1021 ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc
     1081 gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta
     1141 ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga
     1201 tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag
     1261 ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg
     1321 aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca
     1381 tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct
     1441 tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc
     1501 ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg
     1561 gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca
     1621 ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca
     1681 agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt
     1741 acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat gaaactaact
     1801 tgctttgtca ttatga
//
LOCUS       DACDHJ010000283         1773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDHJ010000283 DACDHJ010000000
VERSION     DACDHJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.287"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..790
                     /locus_tag="IV223_002537"
     CDS             <1..790
                     /locus_tag="IV223_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8712780.1"
                     /translation="TILRELKKEFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDP
                     KDYLQQATSAAETIAEQLKRLSVDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETE
                     LLRTTAGNEKYRFITLSSIEHFPLTLANYFFVQKNQLPVTKAFKELAELIGDVNESYF
                     FDQIEVIVDRIRKNKYKNRRPSIFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPT
                     LDPSLTMEEMAFLKRQIFSELSKKFIPHAFSYIKEYGTVLVSKT"
     gene            924..1049
                     /locus_tag="IV223_002538"
     CDS             924..1049
                     /locus_tag="IV223_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712781.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1183..>1773)
                     /locus_tag="IV223_002539"
     CDS             complement(1183..>1773)
                     /locus_tag="IV223_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712782.1"
                     /translation="SFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDITEAEENP
                     FFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFNHNTLF
                     FFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNP
                     STIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          679 a          265 c          340 g          489 t
ORIGIN      
        1 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
       61 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
      121 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
      181 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
      241 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
      301 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
      361 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
      421 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
      481 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
      541 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
      601 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
      661 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
      721 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
      781 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
      841 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
      901 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
      961 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
     1021 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
     1081 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
     1141 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
     1201 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
     1261 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
     1321 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
     1381 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
     1441 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
     1501 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
     1561 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
     1621 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
     1681 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
     1741 aaaaaataag atcgtcaaag ttgcaaaaaa aga
//
LOCUS       DACDHJ010000284         1768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDHJ010000284 DACDHJ010000000
VERSION     DACDHJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.288"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..861
                     /locus_tag="IV223_002540"
     CDS             <1..861
                     /locus_tag="IV223_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAP8712783.1"
                     /translation="KYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETV
                     TAGVNSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAAR
                     LYDFVSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLC
                     GDIPDNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYI
                     TADSKESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKAL
                     DIIKKHLHIK"
     gene            887..1723
                     /locus_tag="IV223_002541"
     CDS             887..1723
                     /locus_tag="IV223_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8712784.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
BASE COUNT          570 a          232 c          371 g          595 t
ORIGIN      
        1 aaatatactc ttagagataa aaagcttaaa tttgagcatt ggcggatatc tgaggatggt
       61 aagattgttg atccgttcaa caagctaaca tctgtctttc agtgctcagc ggaatttttt
      121 atggagactg tcactgctgg tgtgaatagc aataataatc ttgagtatta taatctatgg
      181 aagacaagat tatcaaaagt ttgctatcca gagttagtct atagcaattt ttatgcaatt
      241 tcagaactag taaaaaaaat tccagatagt gcattagttc attctagtat tttaaatgcg
      301 gctagattgt atgactttgt atctgatgat aaagcattac tcagttttgc taatttaggg
      361 gctgatggta ttgatggttg tttaccaaca tttttaggtc aggcagaagc agataaaact
      421 cgacctgcgt atttaatttc aggagacttg agtttctttt atgctatgaa tagtttatgt
      481 ggtgacattc cagataatgc acatattttc cttattaaca atcattctgg aagtgaattc
      541 catacaaatt ttggtaagtt ttacaacgga tcattaagtg ttgataagca tatagctgca
      601 ggacatattt caaacgcaaa ggattgggca ttagaaaata atataaaata cattactgcg
      661 gattctaagg aaagtctcaa atcggggttg gatgtattta ttaatacatt tgacagtcct
      721 gttttatttg aggtctttac agatccggat acggatacgt ctgttctgga atctttttat
      781 agtataaata aggtaatcac accaaaaatg tattttagaa aggcgttaga tattataaaa
      841 aagcatttac acattaagta gatgtttatt gcgaggtgaa acaaatatgg caataaaatc
      901 ttatattaaa cggttattgc tttatatccg tcatggagat cccaaaataa atttagatgt
      961 gagttattta tctgaaggtg caatgcttgc tggtaagact gtgataatta cgggtggtgc
     1021 aaaaggactt ggttttgcaa ttgcaaaatc atttatagct caaggcgcaa gcgttattat
     1081 tacaggaaga gacatggatt cgttgaataa tgctaaatgt cagcttggag atatgtgttc
     1141 tatcagggcg tttgatataa ctgatgctaa aatgtatcga acgttttttg aagatgcata
     1201 cgagaccttt ggacgtattg atatactgct taataatgct gggatctcgt tacacgaaga
     1261 tggatttatg agtgttactg agaaaacttg ggataagcaa atggatacaa atttgaaagg
     1321 tgtttatttc tttacacagg aatttattaa gtttgttaaa gaaaagaatc aaatgcaagc
     1381 aaatattatt atgatgtcct cagaaagagg cttgtgtggt gatagtattc cgtatggatt
     1441 atcaaaggct gcaattaact ctttagttgt aggcttgtct aagaaatata tttctgaggg
     1501 aattagggtt aatggtattg ctcctggagt aactgcaacc gctatgactg gtatttctga
     1561 aaagggtaat ttgtataatc ctgcaacgat cggttataga tattttctcc cagaagaagt
     1621 tgctcaagtt gctttgtttt tggcaagtag tctttcaaac tgtatcagtg gtgaaatcat
     1681 agcctgtgac caggggcaat atttaagtcg aaattggggg taataacttg ggaatattat
     1741 taaaaaaata taatagcctt agtgagcc
//
LOCUS       DACDHJ010000285         1767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDHJ010000285 DACDHJ010000000
VERSION     DACDHJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.289"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..680
                     /locus_tag="IV223_002542"
     CDS             63..680
                     /locus_tag="IV223_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8712785.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            721..1020
                     /locus_tag="IV223_002543"
     CDS             721..1020
                     /locus_tag="IV223_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712786.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1054..1221
                     /locus_tag="IV223_002544"
     CDS             1054..1221
                     /locus_tag="IV223_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8712787.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1266..>1767)
                     /locus_tag="IV223_002545"
     CDS             complement(1266..>1767)
                     /locus_tag="IV223_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8712788.1"
                     /translation="ASGKDTERPEFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVA
                     FMKQKKVALKILDAQFLDFNTGNELLDTVMFDMFLSLLSYIAQNEREKIRERQRQGVL
                     LAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKI
                     KKANDL"
BASE COUNT          577 a          297 c          351 g          542 t
ORIGIN      
        1 cgtttctgat ttaatctgat tgttttgaaa caattttagt gaaaaaaggg tagataaaaa
       61 cgatgagaaa atttttttat atccgggtca gtagttatga tcaatcgatc gaacgacaac
      121 tgatttccgc gaaggaatta ggaatccctg aagaatatat atttgtagaa aaagccagcg
      181 gcaaagattt taaacgccct gaatatcaat taatgaaacg gatgtttcga aaaggagatt
      241 tactctatct acaatcatta gatagactag gcagaaataa acaaatgatt ttagaagaat
      301 ggcgagaatt aactaaggaa aagcagattg atatttctgt gttagacatg cctctgttag
      361 atactgctag ttacaagcaa ctagatgggt tagagacgct tatctctgac ttaattctgc
      421 aattactcag ctatatggcc gaagacgagc ggaaacgaat taatagccgg caaagagaag
      481 gcgtagaagc tgcgaaacaa aagggtgtaa aattcggcag aaagaaaatt gctttacctc
      541 ctgaatttcc tgcgatctat tctgactgga aagcaggaaa gataactgca gtctatgcta
      601 tggatcaatt gggcatgaaa cgaaatacct tttatcgacg ggtgaaagaa tatgaacata
      661 ccttgcacca cttttcttga tagatataac agctattaga cgggaaggga gagtttgaaa
      721 atggacttaa cgaatcaaag ggtcgttttt actggaacgc tgcagcaaat gacacgaacc
      781 caagccatcg gtttggttca ctctttaaac ggccattgtc aatcaagtgt tacttcaaaa
      841 acaaatttcc tggtttgtgg tcagtattct aagtcgttgt ttgatgattc tctctataca
      901 aaaaaggaac aaaccgcgcg ggacttgatt gcgttaggtc atgagattac cattttatca
      961 gaagtcgaat tttatgcgtt aattagtcaa cagttgaaag aatggcaacg gtatttataa
     1021 tcgttagtaa aatttacaag gaggaaaatt tttatgaagc gaaaatctta tgatcgttgg
     1081 ggcattgaaa taactagttt taatcgaaat attgcttttc acgtggagtg tgactgtggg
     1141 gaattagcaa aaagtgttta tcaaaaaaat tacttttact gtcctaaatg ccatcgccga
     1201 tacggggtac atggaacata gaaacgaaat agtgcggtag aaaagcgaat gaatgtagta
     1261 tatttctaca aatcattcgc tttctttatt ttataaatag ttggtcgaga gatcccgttt
     1321 tcttttgcaa tctctgcaac tggaatgcct tgctttaaca tgtccaccac ggttttaaaa
     1381 actaagcgtt tttgcggatc tgttgagtta atagaatact cgattggtcg tcccttatat
     1441 cggccggctt tttgagccaa taatactcct tgccgctgac gttcacgaat tttctcacgc
     1501 tcgttttgtg caatataact tagcagtgat aaaaacatat cgaacataac ggtatccagg
     1561 agttcatttc cagtattaaa atctaaaaac tgtgcatcta atatttttaa cgctactttt
     1621 ttctgtttca taaaagcaac tgtatttttt atatcttcat aatctcttcc taaacgatct
     1681 agtgatgtaa cgatgacgca atcaccttca cggatataat ttagtaattt ttgaaactca
     1741 ggacgctcag tatctttacc actggct
//
LOCUS       DACDHJ010000286         1750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDHJ010000286 DACDHJ010000000
VERSION     DACDHJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.290"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            383..1633
                     /locus_tag="IV223_002546"
     CDS             383..1633
                     /locus_tag="IV223_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8712789.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          335 c          308 g          529 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaataacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct
//
LOCUS       DACDHJ010000287         1735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDHJ010000287 DACDHJ010000000
VERSION     DACDHJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.291"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..311
                     /locus_tag="IV223_002547"
     CDS             <1..311
                     /locus_tag="IV223_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712790.1"
                     /translation="TEQIVQKRQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRA
                     EQERPDLSFFFIIEGRTIKDLQTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE
                     "
     gene            474..>1735
                     /locus_tag="IV223_002548"
     CDS             474..>1735
                     /locus_tag="IV223_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8712791.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIKNLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTG"
BASE COUNT          633 a          291 c          381 g          430 t
ORIGIN      
        1 taacggaaca aatcgtacaa aagcggcagt tattagataa gaatcgtatg gaacaatcct
       61 taggaagaaa tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg
      121 aattgaaacg agcagaacaa gaacgtcctg acttaagctt ctttttcatc attgaaggaa
      181 ggacaatcaa ggatttacaa accagaaacc gtcagttaat gcgatctagt ggcattttag
      241 ggttaaaacc actctacaaa aaagaattga tcaagattct gtatcgaatg aacaacatga
      301 atgatgagta ggaatggaga agagagaatg aaatatccgt tgatatggtt agaaaaagat
      361 gttccgaagg aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga
      421 aaagggcaat cttttcacca agtaaagaat caaacaagaa gaggtgactg tacatgttag
      481 aagcgattaa aacagctttt tcaccacaaa atgatcaaaa agtacttgat ccattaaagc
      541 aactaggaaa tcgggaaata gaaattttaa aatccaaagg atatgattta gattttttaa
      601 aacaagtgat gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg
      661 gttattcacg gtgtgtgaca gtttatcact acacaaaaaa tcctactctt ttttggttgg
      721 ccaatctcat gaacaatcct catacagtgg ccacactaga tgttagcaca gatgaaaagc
      781 aaaaagtgat tgatcaaatc aatcgcgcat tagatgaatt aaatgatcga tcagataatg
      841 aacgaaaagc gactgaccga aataaaagtg cggaagaata cctggagcta gcggaatatg
      901 cccgaaaatt gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt
      961 acggaaacac gattcaagaa gtagatgaac gggcgcagga actgatcaaa aatctaaaag
     1021 ggatggatta tcgtgcggtg acttatctgc ataaaaccaa acgagattgg caatcgatgt
     1081 ttaccatgtt cgatgaacaa gctgaatggt tcggaaatat caaagggcaa tcactaccat
     1141 cgttaaacat tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggaggta
     1201 cacaatatgg ggaaaccatg acaggcggcc catttgtctg gaatgcgacg tataaaaata
     1261 gtgtacggtt gtctactaat accttgcttc taggaatgat gggagctggt aaatcgaccg
     1321 ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa
     1381 agggaaaaga ctttattcct ttcttaaagg aatataacgg tattatggtg cgtttagacg
     1441 gatctgacgg gatgatcaat cctttagaga tatttgccac acggacgtat gatgaagcat
     1501 ccagtttata tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc
     1561 aaacacactt agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa
     1621 cgatgaaagc agaatttaag acctacttaa atcgttttta tgaagagtat ggaacagtac
     1681 cacgcggctt tacttcctca aattcgcgtt caaatactga gacgcaagtc acagg
//
LOCUS       DACDHJ010000288         1735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDHJ010000288 DACDHJ010000000
VERSION     DACDHJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.292"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..311
                     /locus_tag="IV223_002549"
     CDS             <1..311
                     /locus_tag="IV223_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712792.1"
                     /translation="TEQIVQKRQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRA
                     EQERPDLSFFFVIEGKTIKDLQTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE
                     "
     gene            474..>1735
                     /locus_tag="IV223_002550"
     CDS             474..>1735
                     /locus_tag="IV223_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8712793.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTG"
BASE COUNT          633 a          292 c          380 g          430 t
ORIGIN      
        1 taacggaaca aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcct
       61 taggaagaaa tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg
      121 aattgaaacg agcagaacag gaacgccctg atttaagctt ctttttcgtc attgaaggaa
      181 agacaatcaa ggatttgcaa accagaaacc gtcagttaat gcgatctagt ggcattttag
      241 ggttaaaacc actctacaaa aaagaattga tcaagattct ttatcgaatg aataatatga
      301 atgatgagta ggaatgggga agagagaatg aaatatccgt tgatatggtt agaaaaagat
      361 gttccgaaag aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga
      421 aaaggaaaat cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag
      481 aagcaattaa aacagctttt tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc
      541 aactaggaaa tcgggaaata gaaattttaa aatccaaagg gtatgattta gactttttaa
      601 aacaagtgat gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg
      661 gttattcacg atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg
      721 ccaatctcat gaacaatcct catacagtgg caacactaga tgttagcaca gatgaaaagc
      781 aaaaagtgat tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg tcagataatg
      841 aacgaaaagc gactgaccga aataaaagcg cggaagaata cctagaacta gcggaatatg
      901 cccgaaaatt gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt
      961 acggaaacac gattcaagaa gtagatgaac gagcacagga actgatcgaa aatctcaaag
     1021 ggatggatta tcgtgcagtg acctatctgc acaaaacgaa aagagactgg caatcgatgt
     1081 tcaccatgtt cgatgaacaa gctgaatggt ttggaaatat caaagggcaa tcactaccat
     1141 cgctaaacat tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggtggaa
     1201 cacaatatgg ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg tataaaaata
     1261 gtgtgcggtt gtctaccaat accttgcttc taggaatgat gggagctggt aaatcgaccg
     1321 ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa
     1381 agggaaaaga ctttattccc tttttaaagg aatataacgg cattatggtg cgtttagacg
     1441 gatctgacgg gatgatcaat cctttagaaa tatttgccac acgtacgtat gatgaagctt
     1501 ccagtttata tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc
     1561 aaacgcactt agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa
     1621 cgatgaaagc agaatttaag acctatttaa atcgttttta tgaagagtat ggaacagtac
     1681 cacgcggctt tacttcctca aattcgcgtt caaatactga tacgcaagtc acagg
//
LOCUS       DACDHJ010000289         1733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDHJ010000289 DACDHJ010000000
VERSION     DACDHJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.293"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..213
                     /locus_tag="IV223_002551"
     CDS             <1..213
                     /locus_tag="IV223_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8712794.1"
                     /translation="PENYQKLTKEISFKVDKDSYKEENRITIKNNQKASVPMTGSNGF
                     QTYVLISCLLLGAGALSAVVYFKKKA"
     gene            228..980
                     /locus_tag="IV223_002552"
     CDS             228..980
                     /locus_tag="IV223_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8712795.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            996..>1733
                     /locus_tag="IV223_002553"
     CDS             996..>1733
                     /locus_tag="IV223_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712796.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTH"
BASE COUNT          633 a          291 c          344 g          465 t
ORIGIN      
        1 cctgaaaact atcaaaaatt aacgaaagag atctctttca aagtagacaa agattcatac
       61 aaagaagaaa accgaatcac gatcaaaaac aatcaaaaag cttccgtacc aatgaccggt
      121 agcaacggtt ttcaaacgta tgtattaatc tcttgcttgt tgctaggagc aggggcactt
      181 tctgcagttg tttactttaa gaagaaagcc tagaaggatc aaaaccaatg attatcagga
      241 agcctaagaa aaagcgaatc atgggtaaat ggatcattgc tttctggtta ctttcagctg
      301 taggtgtctt acttttgatg cctgctgaag cgagcgtggc gaaataccaa caaaatcagc
      361 agattgccgc aatcgatcga acaggaacgg cagcagaaac ggattcttct ttggatgttg
      421 ctaagattga gttaggcgat cctgttggta ttcttacaat tctaagtatt tctttaaaac
      481 taccaattta tgacggaacg tccgacaaaa ttttagaaaa tggtgtaggt attacggaag
      541 gaactggtga tattacagga ggaaacggta aaaatccttt aatcgcaggt catagtggac
      601 tttataaaga taatcttttt gatgatttac cttcagtaaa aaagggagaa aagttttata
      661 tcaaagttga tggtgagcag cacgcttatc aaatcgatcg gattgaggaa gtgcaaaaag
      721 acgaactaca aagaaacttt gtcacttact tagaatcaaa tcccaatgaa gatcgagtaa
      781 cgctaatgac atgtacgcct aaggggatca acacacaccg ttttctagtg tatgggaagc
      841 gagtaacctt tacaaaatca gaattaaaag atgaagaaaa taagaaacaa aaattatctt
      901 ggaaatggct tttaggaagt actgtttttc tttcagtcat gatcattggt tcgctatttg
      961 tttataaaaa gaaaaaataa aggagggtaa ccaacatgag aaatagaaaa tggctatttg
     1021 tttgtgcagc cgctcttttt tctttcactg cctacacaca gttgacgttt gcagacagac
     1081 aagaaagcac ggatagcaca gaacaaacaa tccaaacgtc agatagcagc acgattcaaa
     1141 gcagcgatga aaccgtcgat tcgtcaacag ttgattcttc agaagaacct aaaattgctg
     1201 aagacgatca aatagtcaaa cagaccattc acgttcaaaa gattatttct tcagaacaat
     1261 tagacaacca ggggcatgaa aaacaagaag gagtaaatgg cgcgaagttt tctgtttatg
     1321 atgtatcgga cattttacaa aagatggatg ttaaggatct cactactgac caaattgaat
     1381 cccaattaaa aaatcgagtg aaaaaactat ccagtgatca attgaagcta gtttctaatg
     1441 gagaaacaaa gacaattgat caacaaacag gtgtctttga attctcggta gaagtacagg
     1501 caaatcagaa acaagcttat tatattgtca atgaatcaag tcctgaaaac atttctaata
     1561 gcgaggatat tttgctatta acacctgttt ctgataaaaa cggtgaattc ttaaaggatg
     1621 tgtggatcta tcctaaatca gaagcttcgc aacctaaaga agaagtaaaa aaaatcgttt
     1681 ctactggcgt aaagaaaaac ttctttgaga attgttggga ttttgttact cac
//
LOCUS       DACDHJ010000290         1730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDHJ010000290 DACDHJ010000000
VERSION     DACDHJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.294"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            306..1598
                     /locus_tag="IV223_002554"
     CDS             306..1598
                     /locus_tag="IV223_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8712797.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          555 a          289 c          348 g          538 t
ORIGIN      
        1 attttacttt tttatatttt gcactgggat gaaataaatt aaccaaaagt ttctttttct
       61 tataatgtgt atcaatatta aaactgaaaa agatatggat aataggatca aagccctcat
      121 tggaacactc aatatattat cagatttaaa tttcaaatag ataatttcat tgtttagtaa
      181 tatcgaaaga tactttttta atgatgagag tgtaatataa accgtgtaag taaccattaa
      241 tcctaggcag gtggttgctt cgcggttttt ctttttctac aataagatca agaggtgatt
      301 acacgatggc tagaaagaaa agaaatcctg atgccgaaaa gttagctgaa tccattctga
      361 atgcctatca acctgaatct gtcgatgaca tgcaagatgc tttgaaagat gtgtttgggc
      421 ccctttttga aaaaatgctt caaggagaat tgaataatca tttaggttat gatgcccatt
      481 ctaaagagcc taaggaacac gataaccgtc gaaatggcta tggaactaaa acgcttaaaa
      541 ccagttttgg tgaagtagct attgatgttc ctagagaccg ggaagcttcc tttgaaccag
      601 agttaattcc taagagaaaa cgagatgtct ccgacatcga agggaaggtt ctttccatgt
      661 atgcacgagg aatgagtcaa cgagatattg ccgcaaccgt cgaagctatt tatggctttg
      721 atatttccca tgaaatgatt tcagatatca ctgacgctgt ccttcctgaa ttggaagaat
      781 ggcaagcccg ccccctagca aagtgttatg cctttctatt cgttgattgt atgtatgtta
      841 ctttacgaga aaattatgaa gccaaagaat atgctgtata caccattctt ggctatgatc
      901 tcaaaggaaa taaggagatt ttaggattat ggttaaatca aacagaatct aaaaatcgct
      961 ggatgcaagt atttgatgaa ctgaaagcac gtggtgtcga agatgtcttt ttcatttcga
     1021 tggacggtgt ttctggcctc gaagaaggcg caaaagcgat ctttccatcg gtaattgttc
     1081 agcgctgtat tgttcatctt gttcgaaatg cattgcgcta tattccaagt aaggactata
     1141 aggaagtctg ccgagatatg aaaaagttct acggtgcttc ttctctaaac gctgcacatg
     1201 ctgcttttga cagttttcaa aatcggtggt ctcattattc tggtgctgta gatgtctgga
     1261 aacggaactt tgcacatgtt gaacaactat ttgattatgg tagtgccatt cgaaaaatta
     1321 tgtacactac caatgcggtt gaaagtgtcc actccagctt cagaaaagtc acaaaaaaag
     1381 gagcgttctc taatgagaat gcactcctaa aactacttta tttacgcacg aaagaattac
     1441 acgcgaagtg gtcaggtggt cgaattcaaa actgggctat ggtgcttaat cagttaatga
     1501 tcaacgagac cttctcctct agaatgaaaa gtatgcgatt taccttccgt aacaacggta
     1561 ggtttattca actatgtgtc cgatcaaaat ttatctgatt tcaaaatagc cgagagaatt
     1621 atggtggtta aaaactctta actatttaat tacggttttc atagtttttg gattcaggag
     1681 gattaaaacg aattctataa aaaaagattt ggtttaaatg aatgattaaa
//
LOCUS       DACDHJ010000291         1673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDHJ010000291 DACDHJ010000000
VERSION     DACDHJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.295"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..372
                     /locus_tag="IV223_002555"
     CDS             <1..372
                     /locus_tag="IV223_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8712798.1"
                     /translation="NLDIAKRFIKTGVDILLVPAPYTVPYFMESDLRGIVDYIKDFNR
                     NKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSLNGICLPENIFA
                     LGQAIRGYRHQIIMLGKSNYR"
     gene            391..1641
                     /locus_tag="IV223_002556"
     CDS             391..1641
                     /locus_tag="IV223_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8712799.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT          543 a          253 c          293 g          584 t
ORIGIN      
        1 aacttagata tagcaaaaag atttattaaa acaggtgtag atattttatt agttcctgct
       61 ccatacactg ttccgtattt catggaaagt gatttgcgag gaatcgttga ttatataaaa
      121 gattttaata gaaataaatc tattgaggaa aaagttctag tactaacggc taatggaaca
      181 agccaagact cttctgacca agaaactatt aaaaaaattg cattagcatc aaaagcatgt
      241 ggagctgata ttcaacacat aggggattct ttaaatggta tttgtcttcc agaaaatatt
      301 tttgctttgg gtcaagcaat tcggggatat cgtcatcaaa ttattatgtt aggaaaatct
      361 aattatcgtt aattaaggag gaaatgaaag atgatcgaca aagttcaaaa aatagctgca
      421 aaaattagta atcaaagaca tctactagct ttaagagaca gcatggctgc agttatgcca
      481 ttaatcatta ttggttcctt ttttatgtta ttagcaaatt tcccgataga aggatttaca
      541 aaatggttat ctgaaattgg agtacaatcc tttttgaaca aagcatcaga ttcaacattt
      601 ggtattattg gattagctgt tacctactct ttgggacaca atctagcaaa gacttatggt
      661 gtagactcat tatcagcagg attattatct ttgagtggat atgttttact tacaccttta
      721 ttggatgcag atggttctat aggatttcca acaaaatact ttggtacctc gggtcttttt
      781 gttggtatta ttgtagcact tgtatctaca gaaattttcc ggtggtttgt acttaaaaat
      841 attacaatta aaatgccggc aagcgttcca ccaaatgtaa gccgagcttt cgttgctatt
      901 attcctggct tttttgtcgt tctactatgg tttattgttg ttgtaatttg ttataaactt
      961 ggaattgaaa atgttcatgc tcttattgca gatacactcg caaaaccact atctttactg
     1021 acgaaaactt taccaggaat tatattggtt atacttattc aatgtttctt ttggatgttt
     1081 ggtattcatg gtgcacaggt aacggggcca ataattgaac cgttattatt gcaaaactct
     1141 gatgtaaata gaattgctta tcaagctgga aaagaacttc ctaacattat aacttatgaa
     1201 ttcttatata actttgtttt ttctggtggt gcaggttgtt tatttgcact tgccatttta
     1261 acatttttct tttcaaagag tcaggaaaat aaaactttag gtaagttatc aatcgctcca
     1321 gtaagttttc aagtagcaga gccccttcta tttgggtttc caactatatt aaatataaaa
     1381 atgttaattc catttgtaac tgcaccagta gtaacaacat taattactta tttttcaatg
     1441 agtatgggat tagttgcaaa acctgtagga gcaacaatcc catggacaac tccaccgata
     1501 attgccggat ttttagcttc tggtggacga ataagtggtg cagtgattca agtaataaca
     1561 attgcaataa atgttttgat atactatcca ttctttaaat tagatgataa tgccaagcta
     1621 aaaagcgaaa aaaatgatta gaactaccta tatgtttcta actgtagtta agt
//
LOCUS       DACDHJ010000292         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDHJ010000292 DACDHJ010000000
VERSION     DACDHJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.296"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IV223_002557"
     CDS             complement(<1..425)
                     /locus_tag="IV223_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8712800.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IV223_002558"
     CDS             complement(534..1565)
                     /locus_tag="IV223_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8712801.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          516 a          289 c          288 g          571 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taac
//
LOCUS       DACDHJ010000293         1663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDHJ010000293 DACDHJ010000000
VERSION     DACDHJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.297"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..1355
                     /gene="brnQ"
                     /locus_tag="IV223_002559"
     CDS             3..1355
                     /gene="brnQ"
                     /locus_tag="IV223_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8712802.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          451 a          294 c          334 g          584 t
ORIGIN      
        1 gtatgacaaa aaaattatca tttaaagact atctttttat aggctccatg cttttcggtc
       61 tattttttgg tgctggaaat cttatctttc ccgttcattt gggtcaagaa gcaggcgctg
      121 ctaccttttg ggcgaatcta ggtttcttgg ttaccggtat cggtcttccc ttcttagggg
      181 tgattgccat tggtgtatcg aaaagctcag gtgtatatga attagcaaaa cggataaatc
      241 aaacatatgc gatggttttt accatcttgc tttacttagt tatcggaccg ttttttgctt
      301 tacctcgcct tgcaaccaca tctttcgaga ttggtttagc accatttatc gatcctagca
      361 gacagacgct atatctagca attttcagta ttgtattttt tgtgctggct tggtggtttt
      421 caagaaaacc gacaaagata ttagattatg ttggaaagtt cctgaatcct gcatttttgg
      481 ttttattagg tgctttgctt ctccttgctt ttttgaatcc actaggttct gtaaatcaag
      541 cgcctattca gccaaattat caagagcatg cttttttcac agggtttact caaggatata
      601 acacattaga tgcgcttgct gctttagcat ttgggatcat cattgtcaca acaatccaaa
      661 acatgggtgt aacaaaacct gctgaaatcg ctaaagacac gatcaaatct ggtgcgatca
      721 gcattatatt gatgggaatc atttatactt tgctcgctta tttaggtgct atgagcttag
      781 gtagctttgc tctaagtgaa aatggcggga tcacgctggc tcagattgct gatcattatt
      841 taggtactta tggcagtatc ttattagcat ttatcgttat attagcttgc ttgaagaccg
      901 gtattggttt gattacagct tttgcagaaa cattcacaga tttgtttccg caaagaaatt
      961 acgcattctt tgttgcactt gctagtatcc ttccttgttt agcggcaaac gtaggcttga
     1021 cgaatatcat tcaattttcg ttgcctgttt tgatgttcat ctatccatta gcaatgacac
     1081 tgattttact agtgttagtt ggtcctttgt ttaagcagcg tacggctgtt tatcgaatga
     1141 ctacttattt tacattgatt gcttcgattt ttgatgggtt gaacgcttgc ccagaaacta
     1201 tcaaacaaac acctattgtt caaaatcttt tatatgttgc ggaaagctac ttgccgtttt
     1261 tcaaattggg gatggggtgg atcgtaccag cagttatcgg ttttgttatc ggactgattt
     1321 ggagtttcgc taagaaagaa gaagtagcag attgataaaa gaggttgtga aagagacgtt
     1381 cagccttgag caataaaaag taattttgaa aaatggcttc tcacattttt gcataaattt
     1441 aacttaatac cgagaaactg acttttgaac accgtttatt gagaggtcgt gaaaaagccg
     1501 cttagtttca agtattaaga aatattttgt gaaaatggct tctcacattt ttgcataaat
     1561 ttgacttaat accgagaaac tggcttttga acaccgttta ttgagcgtct ataaactgac
     1621 ctgagtatta gagaaacaaa acggcaaagt agctccatat act
//
LOCUS       DACDHJ010000294         1654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDHJ010000294 DACDHJ010000000
VERSION     DACDHJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.298"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV223_002560"
     CDS             131..505
                     /locus_tag="IV223_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8712803.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV223_002561"
     CDS             complement(619..1248)
                     /locus_tag="IV223_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8712804.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV223_002562"
     tRNA            complement(1382..1454)
                     /locus_tag="IV223_002562"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          582 a          238 c          282 g          552 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atat
//
LOCUS       DACDHJ010000295         1644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDHJ010000295 DACDHJ010000000
VERSION     DACDHJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.299"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..550
                     /locus_tag="IV223_002563"
     CDS             <1..550
                     /locus_tag="IV223_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8712805.1"
                     /translation="TFSSFLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGV
                     SVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTIL
                     IVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAF
                     LFVISFLISPKQKFLRKKERSL"
     gene            547..1497
                     /locus_tag="IV223_002564"
     CDS             547..1497
                     /locus_tag="IV223_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8712806.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1512..>1644
                     /locus_tag="IV223_002565"
     CDS             1512..>1644
                     /locus_tag="IV223_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8712807.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     "
BASE COUNT          610 a          209 c          307 g          518 t
ORIGIN      
        1 tactttcagt tcttttttag cactaggagt tatccttata ggagttgcaa atagctcaac
       61 ggatttattt catattttat ttggaaatat tttagctgta caagatattg ataagtggat
      121 tacaattggc gtttcggttt tagtgttggt tatagtagtt ctatttttta aagaattatt
      181 aatcacttct tttgatccat taatggcaaa agcaataggg atgaatgtca gtttttatca
      241 ttatctattg atggttctat taactttagt agcagtaaca gctatgcaaa gtgtgggaac
      301 tatattaatt gttgcaatgt taattacacc agcagctaca gcatatttat atgcaaatag
      361 cttgaaaact atgatattta tttctgcaac agtcggagca agttcatcat tgcttggatt
      421 gtttattggt tatagtttta acatagcagc aggatcaagt attgttttaa cagcggcatt
      481 cttatttgta attagttttc taatctcacc aaaacaaaaa tttttaagaa agaaggagag
      541 atcgttatga tgagaaaatg gaaagcagta ctgggaagtc tggggatttt gattgctctt
      601 tttatattcg gggcatgttc aataaatagt aaagacaaag ataaagtggc ttcgaacgaa
      661 aaattaaaag tagtagttac taattcgatt ttagcagata ttaccgaaaa tatagcaaaa
      721 gataaaattg atttacacag tatcgtacct attgggaaag atccccacga atatgaacct
      781 ttgcctgaag atgttcaaaa aacttcaaaa gcagatttga ttttttataa cggtgttaac
      841 ttggaaactg gaggaaatgc ttggtttaca aaattagtta aaaatgcgaa caaagaggaa
      901 aacaaagact attttgcagc aagtgatggc atagatgtta tttacttaga gggtcagagt
      961 gagaaaggga aggaagatcc tcatgcttgg ttaaatttag aaaacggtat tatttacgct
     1021 aaaaatattg aaaaacagtt agcggaaaaa gatcctgata ataaaaaatt ctataaagaa
     1081 aatctagata agtatattga aaaattggat tctctagaca aagaagctaa atctaaattt
     1141 gcttcaattc cgaatgataa aaaaatgatt gttacaagtg aaggatgctt taaatatttc
     1201 tcgaaagcgt ataatgtgcc ttctgcttac atttgggaaa tcaacactga agaagaagga
     1261 acaccagatc aaataaaaca cttagttgaa aaattacgca caacaaaagt tccctcctta
     1321 ttcgtagaaa gcagtgtgga tgatagacca atgaaaacag tatcaaaaga taccaatatt
     1381 cctatctatt caacgatttt tactgattca attgcagaaa aaggacaaga tggtgatagt
     1441 tactatgcga tgatgaaatg gaacctggat aaaattgctg aaggcctttc gaaataattt
     1501 ggagggtagg tatggtacaa attaaaacga ctactatact aatttattta ctagcaattc
     1561 aggaattatc aaaaagaaaa aagaaattaa aaaataatga tttagcaaaa gttctaagtg
     1621 ttagtccagc atctgtaagt gaga
//
LOCUS       DACDHJ010000296         1643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDHJ010000296 DACDHJ010000000
VERSION     DACDHJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.300"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..987
                     /locus_tag="IV223_002566"
     CDS             37..987
                     /locus_tag="IV223_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8712808.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(768..1160)
                     /locus_tag="IV223_002567"
     CDS             complement(768..1160)
                     /locus_tag="IV223_002567"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAP8712809.1"
                     /translation="MPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFY
                     FFQENNNSEYSDYIYATLVTLGETSIALLKFLLSLKRVFSIICFFSKIPRSSIFGIWI
                     CIGILRIKVSRKSSLPSVVAKTKRTLEA"
     gene            complement(1204..>1643)
                     /locus_tag="IV223_002568"
     CDS             complement(1204..>1643)
                     /locus_tag="IV223_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712810.1"
                     /translation="YLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDDSQIEYVPN
                     ELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLLVKLNEEIS
                     VEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
BASE COUNT          523 a          300 c          285 g          535 t
ORIGIN      
        1 ttaagggata gaagtttaga aaggactgtt tataagatga aaaatgatat agtgacttat
       61 gcaaacacaa aatgtgaaga aattggcatc agtaatttag aagagtgctt ttttgacaca
      121 aatgacctgt cgcaattttt aagaacggga agaacaaatg ccagtaaact tgccaatgac
      181 ttagttgcaa agaacgttct atttaaaatc aatactcgtc cagtgttatt tgccatcaaa
      241 gagcgctttg aagagcactt tttgattccg gtaaaagatg tatacgaaag tatatctgaa
      301 ttaaaaacag acttggagcc caattctaca gattccattc tacaaaagat gattgggtat
      361 gatatgagct tatccgatgc ggtggaacaa atgaaaactg ctgtcttcta tcctggaaga
      421 gggcttccta tgatgatcac tggagatact ggagttggaa aaagtttttt tgttcatact
      481 gcttatcagt ttatgaaaaa agtgaacgtt attcatgaga atgctccttt taaagtactt
      541 aattgtgcgc aataccataa taatcctgaa ttattgtcgg ggatactatt tggctatgtt
      601 aaaggtgctt ttaccggcgc agatgaagat cgaaatggat tgttgcaaga tgcagacggt
      661 ggagtactgt ttttagacga agtacacaga ttgcctcctg aagggcaaga gaaactattt
      721 gtttatatgg atggcggaac ttattctaga gtcggtgaaa ctatccatca cgcctccaat
      781 gtacgtttag tttttgcgac tacagaaggc aaagaggatt ttctggaaac ctttatgcga
      841 agaatcccta tacatatcca tatcccaaat attgaggaac gaggtatttt agaaaaaaaa
      901 caaattatcg aaaatactct ctttagcgaa agcaaaaact ttagcaaggc tattgatgtt
      961 tctcctaaag tcactagtgt tgcataaata tagtcagaat attctgaatt attattttct
     1021 tgaaaaaagt aaaaaaaatc gtatcccaaa ctttcatcta catttccaga aagatcttca
     1081 tgttgataat caattcctga atcaatgacc gctaccttca catccttaga acccttggtt
     1141 tcttcccaag cggatggcat gctaatctct cttaaattag cttgtttact ccaattagga
     1201 tcgttatcga tttcctaaag aatcagttgt atttggctcc tcaacatcat tcaacggctc
     1261 tagttcaacc acatagtttg gctctgcata attgaccata tcagaagcat taaatgttct
     1321 tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt gatctcgctt
     1381 attataatcg ctttcatttg tcctaagagt caaatctttt tccaccataa cttttacacc
     1441 caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct ttaaactgac
     1501 aattagctca ttgggtacat actctatttg agagtcatct aattggctca taatcctttc
     1561 tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa acatactcac
     1621 cccaacaacc aatcctaaat ata
//
LOCUS       DACDHJ010000297         1639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDHJ010000297 DACDHJ010000000
VERSION     DACDHJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.301"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(185..1504)
                     /locus_tag="IV223_002569"
     CDS             complement(185..1504)
                     /locus_tag="IV223_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8712811.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          301 c          325 g          527 t
ORIGIN      
        1 taattctttg gcacatttta ttgaaatatg tatcactaat atccctatca actgattttg
       61 actaaaaagt tacttcaaac attcactgtt caaaaatatt gaggaataaa ttttgggtgt
      121 aaggcatttt gagcatactt ctccctggat aacttcccag taaaaaaatc gttagattgt
      181 tggattagat ccattgactg gctcgcctca ggcgagcaaa gaccctgtag aattcattct
      241 gatacacatg ataactagat aatttgagat agactcgtct acccgtttgg actaatttcc
      301 cagcaacttt cagaaacttc aatcgaatag aatgaatggt cattcccttt tggacttctt
      361 caaagccaat cgttcttaag aagttgacta agttgtaagc tatcaagctg agcatcattc
      421 ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc aaagtaaaag ccagctttcg
      481 cttctttaat gaagttctcc attgtgccac gtttggcata gatagaaaag atggtatcag
      541 gagaaacatt ttctgataga ttcgtcacga taaattcatg tcgaaagagt agttcgcccg
      601 cttcacgtgt tgagcgtata cacacgcgac gactttgtga ccaggattgt gcttgataag
      661 tggtcgagaa gtactgaact tctcgttctt cccacttttg attatcgcca taaagtactg
      721 atttctcagc tatttgacct agtctacgat tattcttcag tcgaataaca taatgactct
      781 tttttgattc acacgaatca tacacaccag gtgtagcgaa cccgctgtct ccacgaacca
      841 agatgtcagt gtttggtaga gagtgattat agtgctctaa taaaggtgtg agaaactcct
      901 ttacgccttt tgatgtatat tgatttcctg aacgtagttc agcttttaag aagtcaccag
      961 tcaatccgtc aaaagctacc aatggatggt aaccataggt ttggtagtgt gtattataat
     1021 tcgtttgttc ttggtgacca aatgtatctg aatgggtcga atctaaatca atgattaatt
     1081 ccgtatcatt acggatgagg cgtgctttat cgataagttc ttggttcaaa gcttgaagtt
     1141 catggatatt ctcctcagat agtcgatcta aaaatcggga aagtgaagat tgagaagcga
     1201 gttctttcct atctaaaaca gctttaaaca caggatcttg tctcaggaga ttagctgcag
     1261 aatcagctga gtaaccagca attaattgca taatgaactg ctcaagtatg gataagttgt
     1321 catgagtaaa atacgctcgt tcgtcttcaa tgtgaatgtg ttgcttagcc agttcagaaa
     1381 aaccaaagat gttcatcagc tctttcacta ggacgagacc cgaatcactc gataattgac
     1441 cacctgtatg agaaatagtg atatttgaat tgaattttac ttggtttttg tgtaagctag
     1501 tcatgaaaag aactcctttc ttttggttgg tttagtcact ttaaccatag cagaaagggg
     1561 ttcttttttc atcacttaac gggtgaagat gaaaaagtaa ttgtaagctg ccgtgaggca
     1621 gtttcacatg gttttaatt
//
LOCUS       DACDHJ010000298         1616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDHJ010000298 DACDHJ010000000
VERSION     DACDHJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.302"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..267
                     /locus_tag="IV223_002570"
     CDS             <1..267
                     /locus_tag="IV223_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP8712812.1"
                     /translation="DYFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSLENETAKKL
                     LIQFYEEQAADRLQEYVEKLIKNEEISEMIGKRLLLESEKVVKE"
     gene            264..>1616
                     /locus_tag="IV223_002571"
     CDS             264..>1616
                     /locus_tag="IV223_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712813.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     G"
BASE COUNT          537 a          235 c          333 g          511 t
ORIGIN      
        1 gattatttcc taccagatga atatgctaga aatctctatc atggactgca tgatcaatta
       61 gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga gaatgagacg
      121 gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact tcaagaatat
      181 gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg gttgttattg
      241 gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc atagccgact
      301 ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg aaagtcttac
      361 aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt aatacttttt
      421 tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa accatttatc
      481 aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta tttcaaagtt
      541 tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttgaaat
      601 tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta tttatggcca
      661 tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc agtaaaagct
      721 tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct gcttttcaaa
      781 ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg aataaagtag
      841 aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa acagcgaata
      901 tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat aagagtggtt
      961 tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa ctggatgata
     1021 atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat gtttatactt
     1081 cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa tcttctttgg
     1141 cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag ctggtattta
     1201 aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa cggatgaaaa
     1261 gtgctatcga agatatttct caatccttct tgttattggc atttacaatt ttagaattac
     1321 gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga aagatcaatg
     1381 gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa ggctctcaat
     1441 cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat tctcttctta
     1501 gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa
     1561 gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga tttggc
//
LOCUS       DACDHJ010000299         1605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDHJ010000299 DACDHJ010000000
VERSION     DACDHJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.303"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..638
                     /locus_tag="IV223_002572"
     CDS             <1..638
                     /locus_tag="IV223_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8712814.1"
                     /translation="PYIIIGAGGHAKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGK
                     INELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIG
                     YGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQ
                     IGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            589..>1605
                     /locus_tag="IV223_002573"
     CDS             589..>1605
                     /locus_tag="IV223_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8712815.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPL"
BASE COUNT          568 a          221 c          337 g          479 t
ORIGIN      
        1 atccatacat tattattggg gctggcggac acgcaaaagt cattattgaa accttaagat
       61 tagaaaataa gttaatatat ggattttttg atgataagca aaataaaatg caagatctga
      121 aatatcttgg gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata
      181 aatttattgt agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata
      241 tgccaagtga gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt
      301 caattggtta tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg
      361 gagagcatgc tataataaac acaggtagca ttgttgagca tgattgtaga attaataact
      421 atgttcattt atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc
      481 aaataggaat tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag
      541 gcgcagggag cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag
      601 ctaaaattaa gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat
      661 atgggaggaa cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca
      721 ttaggtgaaa atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc
      781 ggagcttctg cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat
      841 gttggaaaaa acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct
      901 attttatatc aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg
      961 agccctgttg cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag
     1021 gcaatcatcg tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt
     1081 gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat
     1141 aagggaattt cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag
     1201 atcatcacaa cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat
     1261 tcaagatttt tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata
     1321 ggttataatt accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta
     1381 ttgaatgata gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct
     1441 ggaattgata gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg
     1501 tctactttaa cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa
     1561 ctaaatgaaa aaaacattga aacaagagct ttgtggaaac cacta
//
LOCUS       DACDHJ010000300         1595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDHJ010000300 DACDHJ010000000
VERSION     DACDHJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.304"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            194..808
                     /locus_tag="IV223_002574"
     CDS             194..808
                     /locus_tag="IV223_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8712816.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(832..1182)
                     /locus_tag="IV223_002575"
     CDS             complement(832..1182)
                     /locus_tag="IV223_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8712817.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1176..1439)
                     /locus_tag="IV223_002576"
     CDS             complement(1176..1439)
                     /locus_tag="IV223_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8712818.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          523 a          290 c          246 g          536 t
ORIGIN      
        1 ttcttgattg acatttattt acaaaaaata ttgccattta aaaagaaaag tgacacatac
       61 tatttcctcc atcattagtt tttgtctata taaggtatac cctaactaga cacgaccgcg
      121 acattgaaaa atctggtttt ctcgtgtatg attagggtgt tttttaaata gttgcagaaa
      181 gtaggtaaaa attttgagag ttgcatacat acgtgtctca tccatcgatc aaaatgaaca
      241 acggcagatt gaagaaatga aaaaattcgg tgcagagaga attttcattg agaaacaatc
      301 tggcgcaacg attactcatc gacctgtttt tcaagaagca ctggatttcg tccgagatca
      361 agatattttt attgtggaag ctattgatcg acttggaaga aattacgacg agatcatcgc
      421 ttcagtcaat tacttgaaga agaaaaatgt gcagctgatc atcacaagtc taccgatcat
      481 ggccgaagca attggcaatc ctctcctgga taaattcatt aaagatttga tcattcaaat
      541 tttggccatg atcgctgaac aagaacgtac cgaatctaaa cgacgtcagg cacaaggcat
      601 taagattgca aaagcgaacg gtgtttataa aggacgtcct aagctataca gtgccaatgc
      661 aaaagatccc caacgtcgtc ttgtttataa aaacatcgtt gaggatctaa aaaaaggtgt
      721 tgcgatcgct aagattgcaa aagactacaa tgttacgaga caaaccgtat atagaataaa
      781 aaaagacagc atggtcaatc acgaatagtt gatcatgctg ctgtttctat tttatttgct
      841 tactttttga aaaactgttc taactttcat caataactcc tcaactaaac tatcatgcgc
      901 tttttccaaa aaaatacact ctctggcttc ataatcaatt gtcgttagtt gatctaataa
      961 tacttttcct gttgtcttca tttcatattc tgagtttata tttacatata gcggatagtt
     1021 tctagttgta ctagaaattg gagcaactgt aacaacgtta ctataatccg caataatatc
     1081 atgacttaaa acaatataag gtcttttacc tgtctgttcg tgtccagatc gaggtgctgt
     1141 attcaataat agaatatcgc cttgtctagg ttttctcacc atttttcatt acctactgct
     1201 tcacctaaat tttgaatcgt cacatgattt ctttcatcag tgtaattttc aaataactgt
     1261 tcaattgtta attcattttc tatattagcc gcttcgataa ttagccgctt ctgaccgttt
     1321 acttcttctt gtattatctt cactttgtct tctgtatgaa ttccaagata ttccataata
     1381 tactttggca atctaaggcc attactattt ccccattttt ttataggtaa catttccata
     1441 gtagacacct ctttctgtat atacatagta taacatacat aagaaaaaac gtatatactt
     1501 aattatcctt ttctattaaa ttagattaac attccatgaa tccataaact atgttaacat
     1561 ttatatgaaa atagtcgaaa ggaaatcata atatg
//
LOCUS       DACDHJ010000301         1594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDHJ010000301 DACDHJ010000000
VERSION     DACDHJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.305"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..724
                     /locus_tag="IV223_002577"
     CDS             <1..724
                     /locus_tag="IV223_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8712819.1"
                     /translation="FINLYAVIYKREGYNSTNKSIWNCIFSLLTFVIISQGRSGILYV
                     VITSVIMLLISLKPSKIIRFGILGIALIACLVSVTQIREVVISSFVESQSSDTGNIAY
                     RIAEQDYFENKLEGHELFGVGIPNNHDARAVEYSGKTIDFESKKYNPYYLEDLGAFEI
                     RYRFGIIAYALYIVALAISIIDAGFRRDKSVAAFAGMAFMVFMALNFNLLAYITARPF
                     GFMISLILIEISAFNDEFIYIE"
     gene            741..>1594
                     /locus_tag="IV223_002578"
     CDS             741..>1594
                     /locus_tag="IV223_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712820.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDA"
BASE COUNT          555 a          211 c          314 g          514 t
ORIGIN      
        1 ctttataaat ctatatgcgg ttatatataa aagagagggc tataatagta caaataaaag
       61 tatatggaat tgcattttta gtttactgac atttgttata atctcgcaag gcagaagtgg
      121 tatattatat gtagtaataa cctcggttat tatgctttta atttcgctta aaccatccaa
      181 gattattaga tttggtatat taggaattgc cttaattgca tgtctagtgt ctgtgaccca
      241 aattagagag gtcgttatat cttcgtttgt agagtctcag agcagtgaca caggtaatat
      301 cgcatataga attgctgaac aagattattt tgaaaataaa ttagaaggtc atgaactatt
      361 tggagttggt ataccaaaca atcatgatgc cagagctgta gaatattcag gcaaaacaat
      421 agattttgag tctaaaaaat ataatcccta ttatcttgag gacttaggcg cttttgaaat
      481 tcgttataga tttggtataa ttgcatatgc gttatatata gttgctttag ctataagtat
      541 tatagatgcc ggatttcgaa gagacaaatc ggtcgccgca tttgcgggta tggcattcat
      601 ggtgtttatg gcgcttaatt ttaatttgct agcgtatatt acagcaagac cgttcggatt
      661 tatgatttct ttaattttga tagaaatatc ggcattcaat gatgaattca tatatattga
      721 ataggtaagg gagagcacag atgaatttag atcgattaaa gaaaatagca aatatgattt
      781 caccaatcat aaaagtgata aatgcgccgt tagcgtattt tagtgggaaa tcagattata
      841 tggagaaggg aatattgtgg cctactgtaa caacctactc caataacagt agaaaagtga
      901 ttcaatatac aactttacct aaaaacgtag acaacatggc tcaaatgcaa tgggctgggg
      961 aaagttttga ggacatatgt atagttcttc aaggtccagt attagaagac ggtgatttta
     1021 cactaaatac cgttaaaatg tataataaat actatcctaa agtaaaactt gtagtgtcta
     1081 cttggatagg attatctgaa gagacagaat gcaagttgcg tgaagctggt gcagatgttg
     1141 tattatgcaa gaaacctgaa aaaacaggtg cgtacaatat taatctacaa cttacttctg
     1201 catacgaagg cgtaaaaatg gcgcaaaaac tggggtgtag ttatgcgctg aaaacgcgaa
     1261 ctgatcaaag actatatgca aatgatgtac taaagtattt ccaaacatta ttgaatattt
     1321 ttccatcttc ggactgtaaa gtagtgaatc agcgattaat atttacgact tatggaaaat
     1381 catttagata tcttccgttt aatttatgtg attttttggt atacggagaa actagtgaat
     1441 tattaaaatt atatggtatt caaagagata accgagcatc agactttggg aaaattattt
     1501 atactgagga aaaagagttt aataaaaaga tatttacact actagaagga aaacaaggtg
     1561 tatctccata tgatgttttt ccagattttg atgc
//
LOCUS       DACDHJ010000302         1586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDHJ010000302 DACDHJ010000000
VERSION     DACDHJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.306"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..1090
                     /locus_tag="IV223_002579"
     CDS             155..1090
                     /locus_tag="IV223_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP8712821.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            1134..1403
                     /locus_tag="IV223_002580"
     CDS             1134..1403
                     /locus_tag="IV223_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712822.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          554 a          220 c          324 g          488 t
ORIGIN      
        1 ttaaggtggg ttctatgtga aaaaaacgta tcaaaagttt ttatggatta gctatatcgt
       61 cctatttatc gttgggtttc tcgtcagtgt gtccgtgatc gtcggaacgc tttttctttt
      121 gttctctgac gaagatctgg tacttaaggg gttaatgagt acgttatcgt gtgtcatgtt
      181 actttattta tgtatacaga tcaacgaaat tatgccccct cattggcaag aaaagaagaa
      241 agaatttaag gaagaaatcg aaataaaatc caatgactgg attgaataca catggcttcc
      301 tctgaaaaga aatagcaaac gaaaggaatt ttgggcattg caattatggc caataggaaa
      361 actttttatt tatatggctt cacttatggt tactttaatc ggttatttgg tggtggtcta
      421 tggggtggcc tattacttgt taagtgagcc aaaacagatc gcggcgtcaa atattttcac
      481 ggcagtaatg acattaatgt gtgtcatgtg cttttctatt tttagttact tactcgtcca
      541 atttatcagg caaaatttaa caatgagtaa acttttcaaa agtttattgg acttacttat
      601 cttaggcagt atgttaacga ctctaacaga tcggttcgaa caatctgaaa aaacggtaga
      661 ggggataaag aacttcttaa cgacgattga tcaatttata aatggatata tgtatttgtt
      721 tgaagtaggc tttattctca ctttgtttgg ttgtgtattt ataaaactca ttaaaagatc
      781 aatcgaaaaa gaaatggtta ttgatctaat tccgacgctg agcggctatg agtattacgt
      841 tgattttgga aagtatgagg agttgatcca tacggatatc gttcacacga gtatccatga
      901 agtaaccgtt aagggcatca gatctacttt aaaagatgga caggagacga aaacaatcga
      961 ccgaagaaaa gaggtctaca atttttataa agttgaagca agtctacgat atggcattgg
     1021 gaaattgagg ttagatggga gtaatattca acaaaacaaa ccatatgttt taggaaaagt
     1081 ggtgatttag aacttcctat gaaagatgaa taaataagga gaatcttttg aaaatgattc
     1141 ttctttacag aattgtgctt atagttggca cggagttaaa aatcttaaag aaggagcagt
     1201 tattcaaagt gaaagaacat cataatcagt tactatgtat taaaacgggt acgatgaaca
     1261 ttcaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg tcgattcgag
     1321 agcaacataa gcaagaaaaa ttaatggcta cttatagcca attgattgat gagtgggaac
     1381 aattcaagga atctaaaaca taaaattcca tttaaacacc ttatttttta aagagcataa
     1441 ggaaacgtga ccccactttt aaaaaaagtg gttggatagg ctgtgcctat cacgtttctg
     1501 aaaaaaataa atgaccccaa aataccaata tataggatag tacaaggata gcaaaggata
     1561 gtaaaaggat agaggtagga tagcga
//
LOCUS       DACDHJ010000303         1557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDHJ010000303 DACDHJ010000000
VERSION     DACDHJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.307"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..200
                     /locus_tag="IV223_002581"
     CDS             <1..200
                     /locus_tag="IV223_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP8712823.1"
                     /translation="DYDLYEVFYSVFKDEIGSDLKKIELIEALLFLSMIPLHGESLNH
                     QMAMLATGLEILGRVVPDIYC"
     gene            288..686
                     /locus_tag="IV223_002582"
     CDS             288..686
                     /locus_tag="IV223_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP8712824.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            711..1427
                     /locus_tag="IV223_002583"
     CDS             711..1427
                     /locus_tag="IV223_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712825.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
BASE COUNT          498 a          202 c          341 g          516 t
ORIGIN      
        1 gagattatga tttatatgaa gtattttact ctgtcttcaa agatgaaata ggttctgatt
       61 tgaaaaaaat tgaacttatt gaggctttgc tgtttttaag tatgattcct ttgcatggag
      121 aaagcttaaa ccatcagatg gctatgttag caactggttt ggaaatttta ggacgagttg
      181 tcccggatat ttattgttaa tgctttgagc tattgttttt ctatttgatc agtgtaaagc
      241 cgggcatata aattgcctgg ctaaaataaa ggagatgtct tagttatatg tttaaagatt
      301 acgcatttgt ttttgatatt gatggaactt tgtgccccat caaaaagaaa gatgagaaat
      361 atgaagattt agttccgtat gctaatatgg tggagaagtt gcgatactat aaggagaacg
      421 gagcaaagat tacactgttc acatctcgca atatgaattc ttatcatggt aatttaggtg
      481 tgatcaatgc aaatactgca aaaatcttgt tggcttggct agataagtgg gagattccgt
      541 atgatgaaat tctttatgga aagccttggc caggccataa agggttttat gttgatgatc
      601 gcacagttcg tccggatgaa tttttatcgt gtaccctgga tcagctcgat gagatttgca
      661 aaaaatccaa attgaaatct gagtaaagga gactttataa agtgaataag atgacggtta
      721 ttattacaat ggctggtctt ggcagtcgct ttcgtaaagt tggctacact gtgcccaaat
      781 ttatgattga agcaaaaggt aaaaccttat ttgattggtc cttagacagc ttgcttgatt
      841 ataattcaca tgtagaaaag tatgtttttg ttgtgcgtaa ggaagatgat gctactgttt
      901 ttatcaaaga acattgcagt ctgtatggaa ttgaaaatgt cgaggtagtt gaaattgact
      961 atatgactga tggacaagcc actacatgta tgcttgcaat tccttattgc gcacctgata
     1021 atgcaattat ggtttataac atagatacat atgttgagcc aggtgagatg aaatacgttg
     1081 atatttctgg agaaggacat attccgtgtt tccatgcgga cggtgatcat tggagttttg
     1141 ccaagttgaa cgaatatggt gatgtagtag aagtaagaga aaaagttaga atttctaaca
     1201 attgtacttt aggtgcatat tatttttctt ctgcaaaatt atatgcatca ctctacgaag
     1261 aatattatgc agatgaaagg catctggaaa agaatgagaa atatattgcg ccgctatata
     1321 attatatgat agagaagggc atgaaggtaa ccattagtat agtagataaa tcaaaagttc
     1381 acgtactcgg tactccagaa gagctcgatg tgttcttaaa tgaataggga aaatataatc
     1441 atagaaaatg ttaattataa agctgcgtgt gtgggggagt actattcgat agatattcta
     1501 aaaatactaa tggctatttt agttgttgtt cgtcatttag gtcaaaacta tttagct
//
LOCUS       DACDHJ010000304         1529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDHJ010000304 DACDHJ010000000
VERSION     DACDHJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.308"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV223_002584"
     CDS             <1..1350
                     /locus_tag="IV223_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8712826.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          604 a          233 c          330 g          362 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccg
//
LOCUS       DACDHJ010000305         1528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDHJ010000305 DACDHJ010000000
VERSION     DACDHJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.309"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            170..526
                     /locus_tag="IV223_002585"
     CDS             170..526
                     /locus_tag="IV223_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8712827.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            536..1435
                     /locus_tag="IV223_002586"
     CDS             536..1435
                     /locus_tag="IV223_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8712828.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          514 a          274 c          316 g          424 t
ORIGIN      
        1 ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat
       61 cataataaac gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta
      121 gttgcatctg atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga
      181 agtgacaaaa gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt
      241 attagatatc gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga
      301 tacggcattt cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc
      361 tgtagtggct gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat
      421 ggatgatcca tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc
      481 aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg
      541 aaaattggcg atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct
      601 gatgcagctt tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga
      661 tacggctaat aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt
      721 atctacgaca tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat
      781 ggaccatttt ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac
      841 cgttctttta gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga
      901 ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac
      961 agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga
     1021 agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat
     1081 gtatcaatct gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc
     1141 attcggagaa ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca
     1201 tcgtcgcttt gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta
     1261 ttattatgaa tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta
     1321 tgatcctatg aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca
     1381 aacacaacat ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag
     1441 cattagtaat gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga
     1501 tccatttatc gtggatatgc tgcttttt
//
LOCUS       DACDHJ010000306         1516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDHJ010000306 DACDHJ010000000
VERSION     DACDHJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.310"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..317
                     /locus_tag="IV223_002587"
                     /pseudo
     CDS             1..317
                     /locus_tag="IV223_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            472..1047
                     /locus_tag="IV223_002588"
     CDS             472..1047
                     /locus_tag="IV223_002588"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8712829.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1090..1287)
                     /locus_tag="IV223_002589"
     CDS             complement(1090..1287)
                     /locus_tag="IV223_002589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712830.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1274..1456
                     /locus_tag="IV223_002590"
                     /pseudo
     CDS             1274..1456
                     /locus_tag="IV223_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          541 a          230 c          296 g          449 t
ORIGIN      
        1 ggggaacttc atctccagat tccgcgcgac cgcaatggcg agttcaagca acagactgtt
       61 cctgcttata gacggacgaa tgacacatta gaggagaccg tcattcacct cttccgaaaa
      121 ggtattacca tgtcggaaat cgcagacttg attgagaaaa tgtatgggca tcactacacg
      181 ccccaaacca tgtccaacat gactaaagtg ctgactgaag aagtaaatgc atttaaagtt
      241 agaactctaa atgataagta tgtcgctatt ttcatgattc cttcagtttt tcagatgata
      301 taccactcaa aaaacaatat gagagatgtt caggaatacc atctctaatg gcttataaat
      361 tggttttagg gatagtagat gttatagaca tatcaaggaa taaacgagca gctgcataaa
      421 agcagatgga attaaggaat aattgaattg taaaagaagg gaataaattt aatggcacaa
      481 ttattttaca gatatggaac aatgaatagt ggaaagagta ttgagctact gaaagtggct
      541 cataattatg aagaacaagg gaagagagtt atcataatga caagcgcaat agatactcga
      601 tcaggatttg gaaaagttga gagtcgtata ggtgtttcga aaccagctat tgcaattact
      661 tctgaaatgg atgtgtacgg agaattagtg gagattattg atcagtacgg aaaagttgat
      721 tgtattttgg tcgatgagtc tcaattttta agtagagaga atattattga tctatcactt
      781 atcgtaatga atgaggaagt accagtaatt tgctatggat tgaagaatga ttttcaaaat
      841 aatctcttca agggatccga agcattattg acatttgctg ataaaattga ggaaattaaa
      901 actatttgcc atttttgcaa taaaaaggct gtaatgaatg ctcgtattgt tgatggtatt
      961 ccagtttatt caggtgaaca gattgtagta ggaggtaatg aagattacct ttcggtatgt
     1021 cgcaaccact actacaacga agattgaaat aaaaaacgtg taatcaaata attgattaca
     1081 cgttttttat tattctgtta aaggttgagg atcagaaatt tttaggtatc tataatcctc
     1141 acctaaaaat ccgttaaaac gaagtaaaac atcatgacca tttaaatcag ttggaactaa
     1201 aattggaatt aatttatcaa cagtagtttt gttgtaaagt tcaaaagtgt tctccattcc
     1261 ttctggttct acaatcgaat ctttcaataa acaaatcaag aaatacagcc gcagaaaaga
     1321 gcagtttcaa aatgaagaat cactagagcg tttcctagta tccatctttg atacatacaa
     1381 tcaaaaattc ttaaatagaa gccataaagg cttccaacaa gtgacggata cattagcttc
     1441 aatgtttact gagtaaccca ttattttgca gaaggacgag ttatttacac aaaattattg
     1501 acgctccctg tttgga
//
LOCUS       DACDHJ010000307         1510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDHJ010000307 DACDHJ010000000
VERSION     DACDHJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.311"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(388..714)
                     /locus_tag="IV223_002591"
     CDS             complement(388..714)
                     /locus_tag="IV223_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712831.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(701..1504)
                     /locus_tag="IV223_002592"
     CDS             complement(701..1504)
                     /locus_tag="IV223_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8712832.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          437 a          269 c          210 g          594 t
ORIGIN      
        1 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
       61 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
      121 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
      181 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaggg tttaagctca
      241 tggtaactat atcacgatat tacaataaca ttatatcacg atatcgtaat attacaatat
      301 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
      361 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
      421 tccttcaatc aagtattctt caggtatgga ttttaatagc gcatcttcta taacgtctgg
      481 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
      541 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
      601 tatatttttc ttggttaatt cgatttcttc tacttctttt ggtttgtagt tctttttatt
      661 tttatttcct agtagtctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
      721 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
      781 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgttc
      841 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
      901 tcgtcatttc tttagaaatg ttataacgct tattatactt agtaaatagg actcctcgta
      961 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     1021 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     1081 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     1141 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     1201 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     1261 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     1321 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     1381 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     1441 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     1501 ccattcatct
//
LOCUS       DACDHJ010000308         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDHJ010000308 DACDHJ010000000
VERSION     DACDHJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.312"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..424
                     /locus_tag="IV223_002593"
     CDS             161..424
                     /locus_tag="IV223_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712833.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            448..636
                     /locus_tag="IV223_002594"
     CDS             448..636
                     /locus_tag="IV223_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712834.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(719..904)
                     /locus_tag="IV223_002595"
     CDS             complement(719..904)
                     /locus_tag="IV223_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712835.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1119..1490
                     /locus_tag="IV223_002596"
     CDS             1119..1490
                     /locus_tag="IV223_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8712836.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT          541 a          206 c          299 g          463 t
ORIGIN      
        1 catactattg cctccgataa ataaagtcca tgatacaaaa taacactttg tgcgaaatgg
       61 cgcaagcagt tttgtggatt tatccggtaa aatctgtatc gattttgaaa ttagaggaga
      121 aacttgctat aatcaaagtg aaagaaggag tgggattttc atggatcatg tagtgttaga
      181 aatcgatttg catttgatca ataatattcg ggtgatttac tatgttgtaa gtaacagtgt
      241 cgaacaacgt gtactgacga ataaaataaa tggtatttta gcaaagaaag acgttcaccg
      301 attcaataat ggtgaaggaa gttattatag tatcccagta gaaaaaatta tttatacgac
      361 agtcaaagtc agagaagatc tagataccaa aaaagcatat gaacctattt ttacaactta
      421 ttgattgaca gcagatggag gaaaaaaatg aatccatatg aacaacttat tcaagatttg
      481 atcgatggaa aaatcgaaaa gattgatgta aaaagagaag aattaatggc ttttcgagaa
      541 gcgtggctga agttggagaa tcgtaaatat gtggtgggaa aagcctcact gaacggcaat
      601 gtgacttatc attacgatcc gacacgttta ttttagtatg aagtattcag aaaagaacca
      661 aaggaggacg atgcagcagc attgtcctcc tttgatttta caaaagtgaa agttttactt
      721 atttgaatag atttttaaat ttgtttttga cactgtcgat atttacatct tttaaaagat
      781 tttgggcttt ctctttataa ggtcctaatt gttctttatg ttcttcgata aatcgttttg
      841 cgccatcgaa attgccgttc ttaatcattt ccttgacttt ttgaaataat tcttcttgat
      901 tcattttgaa cacatccttt gctatcaaca tgatttttta catggacaaa acgagaaaaa
      961 ctgatttatc cacacgtcaa taatatcaaa taacgatcat ttttactaaa aaaacactcg
     1021 attctcgctt ctaaagtagg agaaaaatct ccaacttggg acggatggaa tttctttaac
     1081 tgacccatat actgaatata taacaaggag gtagaaagat gaaagtgatt aaatatttga
     1141 ttggactggc ggcgattagt ggtattttgc tatttggact gaaatattat actgaaggaa
     1201 gttatggaga aatccctggt atacttgatc agctaaatcc attagttgaa aaaggagagg
     1261 tctatgtaaa gacacaaaaa ccagaggaag taaatgaata tggtacagca aggtatgtgc
     1321 agaaagctgc tgatgccaat gggaaagaac gaacggttga atttaatggt ctttctgtgt
     1381 taaaagaaaa tcattatttg aaaattagca ataaaggagc acacatagaa acgtatgagg
     1441 aagttcctag agaaaaagtt ccggagaaag cattagcaat tattgggtaa atcagttttt
     1501 gtattaaga
//
LOCUS       DACDHJ010000309         1506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.313, whole genome shotgun sequence.
ACCESSION   DACDHJ010000309 DACDHJ010000000
VERSION     DACDHJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.313"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..438)
                     /locus_tag="IV223_002597"
     CDS             complement(<1..438)
                     /locus_tag="IV223_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8712837.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMI"
     gene            complement(579..1181)
                     /locus_tag="IV223_002598"
     CDS             complement(579..1181)
                     /locus_tag="IV223_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8712838.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
BASE COUNT          573 a          265 c          258 g          410 t
ORIGIN      
        1 gatcatacgg cggtccacag tgtgaaacca agtctctaac gattttttta aacaaaaagc
       61 taataaagca gataaaaaag cggataagac aatgtgaaaa tactgcaatg aatctcctgg
      121 ttcaataacc gaagatttca aaacaaaaaa atcgccgatg cttgttaagt gatctcctaa
      181 aagcgacgtc aataacccaa aaggaagcgc gtaaaccaat tgttgacgcg aagtcgaaaa
      241 gaagaaaaaa gtaagcatca gcattcctat agcaattaga gcagtaaaaa tccctaaaat
      301 cgaatagacc catccaatca aaatacaaga aatcagaatg actcccgcaa acagatattt
      361 gtatcggatg tgatggtcaa tcaataagat acaaattgtg taaatcaaat attgcaacgc
      421 tatggaccat aaaaacataa agtacagctc ccattgattt ttaccaaatt atagcacaat
      481 tggcgtatta tttttttaat ttttcaggga ttttgaattc agaactcata ccaaagcagc
      541 agccagatac aaaaaagtca gataagttag ttaattgttt aatgattgtt tttttcatga
      601 aagattcctc caaaattcat caagtagttt gtaataggaa gcaacgtagc tatttctaaa
      661 acaatcccaa gtagcaaata ggtagaaaag taagaagctg agaaaaataa cgtaatgaga
      721 gaaaaaatta gaaacatgct gatcgccttc tttaataatg ggggacgttt ctcgggttca
      781 atgggattga ttgctgtacc tcttgatcca tagcgaagca gtaacaaaaa gcagctgatt
      841 aacaaaaaga ccatcattgg gaaggagata gtcaattgca gatgtttcat caccaaggga
      901 acgccccata aataaagtag tgtccacagc agacaaacga attcagagtc ggaatgcaat
      961 ccataagcat atctgcggat gcagatataa gaaagaaagg tgattcctgt ttccgcccat
     1021 gagcctgtca ccagagaaaa gactagaacg accgctagtt ttccaagatt attcaataaa
     1081 acttctaaga caaattttac ttggacatac accatatctt cttccgattt gtctccgctg
     1141 aataaggctt ctaataattt gttcacaact ttatctttca tctcatacgt acctccacca
     1201 caaattttat gctaaaaaaa ataaaaaacg tcttttgttt cataaaaagt ggttttgaca
     1261 tcaaaaccgt catgatctcc taaaaaaatc ttcgttttcc aagaacctta ccaaaaaaag
     1321 tgacagtttt gatacgaaaa gtacttttta tgacaagttt gtgctttttc gagtgattta
     1381 tgttaaaaaa ttactgacct tataaaaggt gatggaggga ttcttatgga aaatgaacaa
     1441 ttgtttgaag tcggcgaaac atatgcattt aaatctgatt tatttgaatc accaataaaa
     1501 ggaaaa
//
LOCUS       DACDHJ010000310         1503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDHJ010000310 DACDHJ010000000
VERSION     DACDHJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.314"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV223_002599"
     CDS             complement(99..1229)
                     /locus_tag="IV223_002599"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8712839.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          423 a          306 c          237 g          537 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttc
//
LOCUS       DACDHJ010000311         1498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDHJ010000311 DACDHJ010000000
VERSION     DACDHJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.315"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1448
                     /locus_tag="IV223_002600"
     CDS             <1..1448
                     /locus_tag="IV223_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8712840.1"
                     /translation="GFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANGGLG
                     LPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPFGV
                     STLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPLIV
                     GTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIRNL
                     IIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKPRV
                     WAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFILT
                     PVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGSFF
                     IVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTESA
                     YFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          403 a          286 c          316 g          493 t
ORIGIN      
        1 agggcttctt cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac
       61 gatttagtta ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca
      121 acgggggact tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt
      181 tgatctacat gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac
      241 gcactgtatt cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat
      301 ttggtgtaag tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga
      361 aaccaaatgt ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg
      421 caggattttc gattttttac atgggtgtca acttaggggc attattagca ccactaatcg
      481 ttggtacaat cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga
      541 tgttctttgg attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta
      601 caacaccgcc gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca
      661 tcgcacttgt tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa
      721 ttgacttttt tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta
      781 tcaaaatgct gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata
      841 tcccattatt ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct
      901 tagctttatt tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa
      961 gctggtatca atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga
     1021 tgtggacgaa acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt
     1081 tatttgcagg attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg
     1141 gaaaagtaag tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt
     1201 gtctttcacc agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac
     1261 aaacaatcgc tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg
     1321 cacgcttcta cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa
     1381 tcgtcggtgg tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta
     1441 ttcattaatc aatgaacata aaaacaggaa ttgcttattt gcagcagttc ctgttttt
//
LOCUS       DACDHJ010000312         1493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDHJ010000312 DACDHJ010000000
VERSION     DACDHJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.316"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..846
                     /locus_tag="IV223_002601"
     CDS             <1..846
                     /locus_tag="IV223_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8712841.1"
                     /translation="KYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQGNTS
                     DVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPADMSF
                     EMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIADWAI
                     VVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQAQEI
                     YGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEEHENW
                     YAEVE"
     gene            847..1395
                     /locus_tag="IV223_002602"
     CDS             847..1395
                     /locus_tag="IV223_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712842.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          561 a          215 c          315 g          402 t
ORIGIN      
        1 aaatatgtaa tcggtaattt taaaggtggc gtaggtaaaa gtacgtgcgc gcaaatgtta
       61 gggtttgaat cagcagttaa tgaaggaaaa agaaccttga taattgactt ggatatgcaa
      121 ggaaatactt ctgatgtgat gaacttaacg catatgaact tcactaacga agaaggaggc
      181 ggagaaggtg aaccgattgt ttacgaaaat actgtgactg acgtgttgat aaacaatctt
      241 ccacctaaag aggctgtcta taaagtaatc aataacctct atatacttcc agcagacatg
      301 tcctttgaaa tgtatgatga ttggatcaaa gaccaatata atgattcatt atcccaattc
      361 aaatatatga aagaaaagct agaaccatta tttgatgagt ttgacgtcat ctatttagat
      421 gtcccacctt ctatttctgt atatagtaaa tccgcgatgt acattgcgga ttgggcaatc
      481 gtcgtcttac agacccaagt aaaatcgatg agaaatgcga tgcaatatct tgagtatatg
      541 gacttttttg tgaaagaatt tgatacaaat ttatatgttg ctggtgttat tcctttcatg
      601 ttggaaagcg gtgacgcagt agatcaggaa atgtataaac aagcccaaga gatttatggg
      661 gaacatttga ttaaaaatgt tgtactcaaa aatgcgcgct taaaacgata cgacggatca
      721 ggtattacga tggagaagac aaagaaaggt ctcttaaaac aatgggatcg caaaagccac
      781 gaattattta tcagtatttt gaaagaattg gaagaacacg agaattggta tgcggaggtg
      841 gagtaattgg ctgacatcaa aggacttgat atttttggga acaacaaagg actcgccaaa
      901 ataaaaaaag aagcagaagc aaagaaaaag cagcaagcaa ttacaccatc tgaaaataag
      961 caattagaag tgcaccaagt tcaagaaata aaaacttcct ccaatgttga aagacagagt
     1021 agaggaagaa caaaaaaagt tggcgcacca atcagaaagt ttgataaggt ttattccacc
     1081 aagcatcctt taaaactgtc gactttgtta aatgcgactt ccagagtttt agtagaaaag
     1141 tatgagacta atatgacaag agatgaattg cttagaaaag ctttagatga gtatattaaa
     1201 cagaatctta cgcaagaaga taaacaagat ttgtttaatg acgtggtgaa agaactagac
     1261 ttgttcagag aaaaatatcc aattgttcca gaattagatg atcgtggaga aataataaga
     1321 actaccagtc agataaaaca agataccgaa gactcactgc gcaaagggtg gggaatgaat
     1381 aataaagtta gataataatc aaagaagaac taagtaacta cctttaaacg gtagttactt
     1441 agttcttctt tgatttgcgt gaaatagagg ggatttattt ttctggttac gtg
//
LOCUS       DACDHJ010000313         1475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.317, whole genome shotgun sequence.
ACCESSION   DACDHJ010000313 DACDHJ010000000
VERSION     DACDHJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.317"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(128..460)
                     /locus_tag="IV223_002603"
     CDS             complement(128..460)
                     /locus_tag="IV223_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712843.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(510..>1475)
                     /locus_tag="IV223_002604"
     CDS             complement(510..>1475)
                     /locus_tag="IV223_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712844.1"
                     /translation="KLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
BASE COUNT          371 a          287 c          244 g          573 t
ORIGIN      
        1 tagtaattaa gactccctct tctgtacagg atttatacgg aaataagtcc aagatagagc
       61 gctcttctgg ttttccaaac tgcaaaatgg atttcctttc aactgaattt tgtcctttga
      121 ctcgcattta ttcatcctcc ttatacgtac catgataatt tcggttagga tctaacgcat
      181 gataatggtt cttgtccgca aaaaaaatag actcgataat cgcaaaaatt tgtcgttgac
      241 ctgggttatt atagggtcgc caaacaatca ctaacgctat cgcaatccat aagccataat
      301 tcaagagtgc aaaaaagagt ggtaagtgga caagattatt gaccattttc gcccaaaaaa
      361 agcagccacc aagcgttaat agatctaaaa agaaaaaagc gaaaaacttc cattctccac
      421 taaatcttgg agggtttttc atgtttatat atttatccat aattgatttc tccttttcat
      481 ataaagaaaa ggcctaactt gtaggccttt tatggtttga tatcctgttt gatatccctc
      541 aatagttgtt ctttcaatgc attgttcctg tttttagttt ctcttgtcac atttattttt
      601 gttggtgcag taaatgccac atttgctggc ttgtaacctg tatgaacgtt ttgaataggt
      661 tttggtgcag cttctcttgt cacaacattt tgaacggacg cattccctgt ctcaccactt
      721 tcagataaat tgtaagaaac agttgtcaga tttccatttt caggcaaacg acttggtttc
      781 atttgaacag tacctgcagg tgattgatga acaaaacttt gatcattatc cttgaattga
      841 ttcaaacgac tatctttcat tggtaaattt tgtgccttag gtaaaccaat tttcgttgat
      901 ttttcagttc caacttcgat tccattatca tttggcagaa cgatggaatc ctcatccgtt
      961 tgtacttctt gatttcgttt tggtaaatct gaaatgcgtt tttcattttc ggagttaaca
     1021 gatctatacc gatattgttc tggatcagtt tttggtaact gtccctcatt ttctgctttg
     1081 tcttcttgat ttacttttga taaatctgaa attcgcttct cattttcaag gtcactgtta
     1141 ggtttatagc gatattgttc tggatcagtt tttggtaact gtccattttt tccttcttct
     1201 gaatttcttt cttctatgga atttttctct tcaatcgaat tttcattaga gtgcaacgct
     1261 tcttggtgat ctttgactga aggaatcact ataccttctt gattttcttt ttcggttgtt
     1321 gtattagctt tcactgctgc attgacttgt ccttcaccat acgcatcttt tagtccttct
     1381 ttgacttctg aagctttttc tttttgtttt tctagtgctt cggatggatt cttcacagct
     1441 tcagccaatt ctttagctct cgtgccgtct aattt
//
LOCUS       DACDHJ010000314         1461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDHJ010000314 DACDHJ010000000
VERSION     DACDHJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.318"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            41..868
                     /gene="panB"
                     /locus_tag="IV223_002605"
     CDS             41..868
                     /gene="panB"
                     /locus_tag="IV223_002605"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP8712845.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            883..>1461
                     /locus_tag="IV223_002606"
     CDS             883..>1461
                     /locus_tag="IV223_002606"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP8712846.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSL"
BASE COUNT          562 a          153 c          328 g          418 t
ORIGIN      
        1 aattagaaaa gtatttagga gagagttagg agagaaatag atgaaaaata cagcagttac
       61 atttaaagaa tctaaattaa gaaatgaaaa attaacaatg cttacagctt atgactattc
      121 aacagcaaaa ataattgatg aagctgggat aaatggaata ttagtaggag attctcttgg
      181 tatggtatgt ctaggacatg aagatacact ttcagtaaca atggaagaca tgattcatca
      241 tactagagca gttacaagag gagctaagaa cactttagtt gttgcagata tgccatttat
      301 gtcttatcaa acttcagtat atgattcagt agttaatgca ggaagattaa taaaagaagg
      361 acgagctcag gttgttaaat tagaaggagg gatagaagta tgcgataaaa tagaggctat
      421 agtaaaggct tctatacctg ttatggcgca tataggttta acacctcaat ctgttaatgc
      481 atttggagga tttaaggtac aaggaaagga caaggaagct gctaaagaat taataagagc
      541 tgctaaagct gtagaaaaag caggagcatt cgctgtagtg ttagaatgtg ttccaactaa
      601 gcttgcagag ttaatttcta aagagatatc aatacctaca attggtattg gagcaggtgc
      661 agggtgtgat ggacaaattc ttgtatatca agatatgttg gggatgtata gtgattttac
      721 ccctaaattt gtgaagaaat atgctaattt atcagaagag atgaataaag catttacaaa
      781 atatatagag gaagtaaaag atggagtttt tccaggtcca gaacatgggt ttaaaataag
      841 tgatgatgtt ttagaaaaat tatattaaaa gggtcaagaa atatggttat atttaaaaca
      901 gtgaatgatg ttagaagtca agtaaaagag tggaaaaaac aaggcttaaa ggtaggatta
      961 gttcctacta tgggatattt acatgaaggt catgaaagtt taattagaaa agcatcaaaa
     1021 gaaaatgata aagttgttgt aagtatcttt gtaaatccaa ctcagtttgg gaaaaatgaa
     1081 gatttaggta gttatccaag agacttagaa agagatatag aagtatgcac aagaggtaga
     1141 gcaacagcaa tatttaatcc tgaagttgaa gagatgtatt gtgacaatgc aagtactttt
     1201 gttaatataa ctggattaac agaaggttta tgtggagcta gtaggccaat acattttaga
     1261 ggggtttgca ctgttgtaag taaattattt aacataatac cagcagatag agcttatttt
     1321 ggagaaaagg atgcacaaca gttagctgta attaaaagaa tggttaggga ccttaatatt
     1381 gatatagacg ttgtagggtg tccaattata agggaagagg atggactagc taaaagttct
     1441 agaaacacat acttaagttt a
//
LOCUS       DACDHJ010000315         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDHJ010000315 DACDHJ010000000
VERSION     DACDHJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.319"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..841
                     /locus_tag="IV223_002607"
     CDS             <1..841
                     /locus_tag="IV223_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAP8712847.1"
                     /translation="KQYIDYSHRKIDASKKNSFVSIKNDYDYFLVGSDQIWNPKYAAN
                     KGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLYVTEWNKFKSISVRENQAAKIIEDL
                     TGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENYILSFFLTNPPKDTVDVLDKVAKDL
                     KIGVLTLDASWEYFQDITPNHFVYLISHARMIYTDSFHCTVFSLLFGKPVVVFDRFDS
                     EKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIASEPEYDIDKLMVSERECVKAYLKE
                     YLQ"
     gene            864..>1455
                     /locus_tag="IV223_002608"
     CDS             864..>1455
                     /locus_tag="IV223_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712848.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEF"
BASE COUNT          495 a          197 c          297 g          466 t
ORIGIN      
        1 caagcagtat atagattatt ctcatcgtaa aattgatgca tctaaaaaaa acagctttgt
       61 gagtataaag aatgattatg actatttctt ggttggtagc gatcaaattt ggaatccaaa
      121 atatgcagcc aataagggat ttgaactttt atcatttgtt ccaaatgaaa aaagagtttc
      181 attatcaacc agtttgggag ttccatctat accaagtgat ttagaaaaat tatatgtgac
      241 agagtggaat aagtttaaat caatatcggt acgtgaaaat caagcggcaa aaataattga
      301 ggacttgaca ggcacaactg cggctgttca cattgaccca actttaatgt tggagcgttc
      361 ggaatgggaa gcctttcaaa aaaaaccaga caatattgat gaaaattaca tcttgtcgtt
      421 tttcttaact aatccgccta aggatactgt tgatgtatta gataaggtcg ctaaagattt
      481 gaaaataggt gttctcacct tagatgcgtc ttgggaatat tttcaagata ttactccgaa
      541 tcattttgtt tatttgattt cacatgcaag aatgatttat acagattcat tccactgcac
      601 agttttttca ttgctatttg gaaaaccggt tgtggtattt gaccgttttg atagtgaaaa
      661 aatgaattct aggttaagta cattgttaaa taaagtaaat aaagcacatt tttgggtagg
      721 aaaaaaagct gatgaagcaa tgattcgtgc aatagcatct gaacctgaat atgacataga
      781 taagctgatg gtatcagaga gggaatgtgt aaaagcttat cttaaagagt atttacagta
      841 aaattttgaa atgggatgaa aatatggagc atttaagtga ttgttttggt aaaatgaaga
      901 ttgttacgac ttcatgcgca agagaatatt ttaaggatga tttggataaa tcagagtatt
      961 catgttttgt tgattctcga gcagctatgt ttttcgcaat gggacaagct ttagctaaaa
     1021 gaaggaatgt agttgtaatg cttgctgatg aattgacatc gtgttatacg gcactaacag
     1081 aatgtgcatt tcaacaagtt gatttaattg tgtttgcttt tcagacagtc agacatgaaa
     1141 ttgatttagc atctttgaag aattgtggtt gcaatctaat taactgtagc gcccaagacg
     1201 atattgatag agttgtgtcc aacactaagg ggttaaatca gacaacagta attaaaatca
     1261 atatgaattg tgagtcggat aaagatcata tatgtgagta tatagacctg ccagacatac
     1321 ctgttttttt ccacggtagc ttatactcac ctgcaagtaa aaagaaatat gagcagtatg
     1381 gacaggtatc gaagtattta ggctatctcg tggggggaaa agatgcaata ctcataattt
     1441 ctcacaatga gtttt
//
LOCUS       DACDHJ010000316         1441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.320, whole genome shotgun sequence.
ACCESSION   DACDHJ010000316 DACDHJ010000000
VERSION     DACDHJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.320"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..763
                     /locus_tag="IV223_002609"
     CDS             <1..763
                     /locus_tag="IV223_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8712849.1"
                     /translation="KDHNIEYDLVLQEAESSRGLKKKLLQLEYKRTRKYEEAAVNRAD
                     YCFSVCDANTEVLKKINDNSYTMLPTYEMLTGVKKNIEKNTILYLGNLSWKSNMQGLV
                     WFLESIYPNIQQRIPDVKITIVGSGPVGTDFSKYSGVDYRGYVKDISHIYDDQAVLIV
                     PLLEGSGIRIKILEAFNNEIAVVSTLVGCDTIGAKDGSELFIADNEKDFATAVVRLLE
                     DMNLNEKVRKNAKLFLKEKYSLTARQDEFSAIINS"
BASE COUNT          498 a          169 c          316 g          458 t
ORIGIN      
        1 aaaagaccac aacatagaat atgatttagt cctacaggaa gctgaatcaa gtcgaggttt
       61 aaaaaagaag ttgcttcagt tagagtataa gcgtactcga aagtatgaag aggccgcagt
      121 aaatagggcc gattattgct ttagtgtgtg tgatgcaaat acggaagtac tgaaaaagat
      181 taatgacaac agttatacga tgcttccaac ttatgaaatg cttacagggg tgaaaaaaaa
      241 tatagagaag aacacgatcc tatatttggg taacctttct tggaaatcca atatgcaagg
      301 attagtttgg tttttagagt caatttatcc aaatattcaa caaagaattc cggatgtaaa
      361 aatcacaatt gtcggtagcg ggccagttgg aacagatttt agtaaatatt caggcgtgga
      421 ttatcgtggg tatgtaaaag atatttcaca catttatgac gatcaagcgg tgctaattgt
      481 acctttgtta gaaggtagtg gtataagaat aaaaatatta gaagcgttca ataatgaaat
      541 tgcagttgta tctacattag ttgggtgtga tacgattggc gcaaaagatg gaagtgagtt
      601 atttatagca gataacgaaa aagattttgc aacggctgta gttagactgc ttgaagacat
      661 gaatttgaat gaaaaagtaa gaaagaatgc aaaattattc ttaaaagaaa aatactcctt
      721 aacggctaga caagatgagt ttagtgcgat aattaacagc taatatatat ataagcttat
      781 ctgatgatga tttataggag gaaatatatg gagatggctg agagaaagga aaagaaactt
      841 ggaaataaga atatcatatt attttagaaa actcctgtta ttttatgttt gtgtgattgt
      901 tgcggctatg agttaccacc ttacaggcaa ttccaatgta gcgtcattaa ttgctgtagt
      961 aggagtagtt tatgctattg taaaggcgtg taataacgag tatctggcta tatgggttat
     1021 gtttgtatta ggttctgata tatgggggat agtgccatat attaacattg gtagtggtgt
     1081 gttgcattgg tcagatttta atgttatagt agcgatattt ctctgcttga gattcatcaa
     1141 agttaaagct aggagtagat acgggattgt ctatagtgta atgcttatat ttattttgat
     1201 tgcatgtgtg caatcgcatt caatctatgg acaagatgta catacaacgc tcttaacagc
     1261 tagagaattg ctagttgtaa tatccttttg gccgatagta aaaatagtat attcagaaag
     1321 aataactaaa aatgaattca ttgatttagt gtgtaaattc acaagatgga caatgtgtat
     1381 atattggttt gaatggattc ttgttaatat gggattcaat ataacctttt tacagacagg
     1441 t
//
LOCUS       DACDHJ010000317         1439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.321, whole genome shotgun sequence.
ACCESSION   DACDHJ010000317 DACDHJ010000000
VERSION     DACDHJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.321"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(36..1200)
                     /locus_tag="IV223_002610"
                     /pseudo
     CDS             complement(36..1200)
                     /locus_tag="IV223_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          489 a          261 c          232 g          457 t
ORIGIN      
        1 tattctctag taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt
       61 aacttcctga taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa
      121 gctaacttca tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt
      181 ccttcaatcg acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac
      241 tgttccccaa taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt
      301 atagactatt ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct
      361 tacttcatga ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata
      421 aagtcctccg ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg
      481 atctctctga gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa
      541 cagtaataaa acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt
      601 catgaaaacg aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca
      661 aggaaattga atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt
      721 ggataagagc caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc
      781 tttaaaaaag aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt
      841 tgtcaggtca ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg
      901 taaggtcgtt ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata
      961 ccaaaagtat gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa
     1021 ccggatgctt tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc
     1081 aacggctatt acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact
     1141 gactgctaat ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat
     1201 aactatcggt ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc
     1261 aatcgtacgg tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct
     1321 ttttaaaaat tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca
     1381 aacatataag atataactaa cgattaaatt attacttatc caataaaaaa tgactgccg
//
LOCUS       DACDHJ010000318         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.322, whole genome shotgun sequence.
ACCESSION   DACDHJ010000318 DACDHJ010000000
VERSION     DACDHJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.322"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            52..462
                     /locus_tag="IV223_002611"
     CDS             52..462
                     /locus_tag="IV223_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8712850.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            476..832
                     /locus_tag="IV223_002612"
     CDS             476..832
                     /locus_tag="IV223_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8712851.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          531 a          211 c          268 g          427 t
ORIGIN      
        1 tttaaaagaa attactgtag aattggatta taattaaaaa ggagtgagaa aatgataaaa
       61 gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat
      121 caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa
      181 aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt
      241 tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc
      301 aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc
      361 ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga
      421 aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga
      481 agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga
      541 acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatctaa gtacagaaga
      601 agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc
      661 tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt
      721 tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg atgaagttct
      781 atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac
      841 tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa
      901 gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat
      961 gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa
     1021 tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa
     1081 aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagttgtt
     1141 aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa agttcttgac
     1201 aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa aacgaaatca
     1261 aaaaaacttt taaaaaagag ttgacaatta cttgtctctc tgatacgata taaaagttgc
     1321 tacggcagcc aaaaacgaat cggggaacac tcctgatgaa gtagaccttt gaaaactgaa
     1381 caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca acaaaca
//
LOCUS       DACDHJ010000319         1435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.323, whole genome shotgun sequence.
ACCESSION   DACDHJ010000319 DACDHJ010000000
VERSION     DACDHJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.323"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..1370
                     /locus_tag="IV223_002613"
     CDS             39..1370
                     /locus_tag="IV223_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8712852.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          468 a          218 c          260 g          489 t
ORIGIN      
        1 aaagtaatcg attacaataa gcataaagga gaaaaaatat gggcaaaagt agcaggaagg
       61 acattgtatt tgaaaaattt ggtatagttg cgactaagtt ggggaatcag atccatttaa
      121 gaactttgcg agatgcgttt gctacattta tgccatttat gatgttagca ggatttgtga
      181 cacttattaa ttcagtgatc ttagaaccaa caggattcat gggggaaatt gtcgatacta
      241 gtacattgac cacgttacaa caaattggta cttcaattgc caatggtaca ttaagcataa
      301 ctacattatt agttgtagca gcagtatctt atcatatgtg tgcaagcaga aattatacaa
      361 atcatattgc agcggtatta gttgctattt cgacatttgt tgtattaaca cctatgcaga
      421 tgatgtttac tcctgaaaat gcggataaag ctgttgaagt gacgggtgtg atacctatat
      481 cctatacagg agcttcagga atgtttgtag gtatatttgt tggattagcg gctacagaat
      541 tatttattaa actttctaat aataagaaac tacagattca gctatccgga aatattcctc
      601 ccgctgtttt aaagtctttt aatgtattaa tcccaatcat tattacgatt acctcgtttg
      661 ctttattgtc atttataatc attcaattgt ttaatatgga tataaataag ctaattacca
      721 cgatagttac agagccatta agtaaaatta caactggttt accaggattt ttgttaatta
      781 catctattgc taatctattt tttggatttg gtatccatca agcagtaata tcaggttcat
      841 tgttagatcc gtttttgatt cagaatatgc aagaaaatat ggcagcttat gcaaacaaag
      901 agcaaattcc acatattatc aatatggcct ttaaagatac ctttgcggta atgggtggat
      961 cgggaaatac gattgcttta ttgattgcaa tatttatctt cagtcgtaga aaagattata
     1021 aggactttgc taaattatcg gtaactcccg ctatattcaa tataagcgaa cctattattt
     1081 ttggattgcc tattgtattt aatatcagtc tgattattcc ctttgtgctt gcaccgattt
     1141 tttctttgac agtagcatat tttgcaactg ctgtgggatt aattaatcat gtagtagtgc
     1201 aaattccttg gactacgcca ccagttatat cgggattttt agcaacagcc ggagactggc
     1261 gagcagctgt tttacaaata ttaattatag ccgcaagtgt gtttatatat ttaccgtttt
     1321 tacgaattga cgaacacgtg acaactaaat taagtgaaaa tgaaacataa tttactatga
     1381 gataacaaaa attaacacaa gaagtcgttg gaatataaat agccagtaat ttcaa
//
LOCUS       DACDHJ010000320         1434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.324, whole genome shotgun sequence.
ACCESSION   DACDHJ010000320 DACDHJ010000000
VERSION     DACDHJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.324"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            554..1417
                     /locus_tag="IV223_002614"
     CDS             554..1417
                     /locus_tag="IV223_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8712853.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT          504 a          212 c          284 g          434 t
ORIGIN      
        1 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
       61 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
      121 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
      181 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
      241 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
      301 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tgtgtcttcc
      361 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
      421 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
      481 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaagcaag
      541 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
      601 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
      661 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
      721 attggctttg gctaatcgcg atctcctaga tggatcaaga gtgacagaaa cggcgtttag
      781 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
      841 gccttcagaa atcagcagat acggcataca aaaatctttt ccacgcttga cattttttat
      901 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
      961 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     1021 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     1081 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gcccgcttgg aagaaaaggg
     1141 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     1201 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     1261 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatga
     1321 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     1381 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atat
//
LOCUS       DACDHJ010000321         1433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.325, whole genome shotgun sequence.
ACCESSION   DACDHJ010000321 DACDHJ010000000
VERSION     DACDHJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.325"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..191)
                     /locus_tag="IV223_002615"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="IV223_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            212..1414
                     /locus_tag="IV223_002616"
     CDS             212..1414
                     /locus_tag="IV223_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAP8712854.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
BASE COUNT          520 a          220 c          268 g          425 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 agggtcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 gtggcaacat tgtgtacttt tctttgcaat cttgtgtaca aaactattgc aaaacacagc
      241 gcataagaat ccttacaaaa cagtagatga agcaacccaa ttagatcttg ttatagttgt
      301 taatcagctc ttaacgggtt acgattcaaa atgggtcaac accctatact tagataagtc
      361 acttgaatat cagaacataa ttcaggcttt ttcacgcaca aatcgtctta atggtcctga
      421 aaaaccgttc ggaaccattc gttattatcg taaagcacat acgatggaac agaacattga
      481 agaggctatt gagttatact caggcaaaca agcaaaaggg ttatttgttg aaaaattata
      541 taataactta gaaaagttga acgatatatt cgaagaaatt aagtccgtat ttaatatttc
      601 tggcatagaa aacttcagtc atttaccgga ggttccaggg gaaattaagc aatttgtaaa
      661 tctttataat caactaatcc attgttatga atcagcgatg atacagggat tcacatgggc
      721 aaaatcgact tatgaaatcc aggattatga acctataaca gtagtaatca cagaatatga
      781 atttaatatc ttggcacaac gttacagcga gatagagaga gaacctagag acggaaatga
      841 tagtatagcg tatgacattg attaccaatt acctgaaggt agtcgtagtc gtattaatta
      901 tgagtattta aacacaaact tcaaaaaata catgaatata aaagaagaac gtgattcgaa
      961 tttaattcaa tcattgttaa acgacttaca tcgcacattt gcgcaattac cagtggagtt
     1021 acagccatat gctgagatga ttctaaatga tgtaaataat ggggaattag ttatcaaaga
     1081 aggatgggaa tttactgatt acctcaatga atatcagctt agtgaagagg acgattttat
     1141 ccaagattta gtggattcaa ccaatattaa tgaggcatta ttacgtgaga tgttggattt
     1201 acgcttaacc gaggtaaaca ttaatgaata tagtcgattt gataaattaa aatcaagtgt
     1261 taacgtaggt catttcagtg gatatatcga gaaaaattta ggtaaaatgg taataccaat
     1321 caaggtaaat atgctaattg acagattatt gcgtgcattt ttattagaag atgtattcga
     1381 tgttacagag tacataaaga attactttaa ctagagtaca cctacatctt ata
//
LOCUS       DACDHJ010000322         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.326, whole genome shotgun sequence.
ACCESSION   DACDHJ010000322 DACDHJ010000000
VERSION     DACDHJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.326"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV223_002617"
     CDS             complement(150..1337)
                     /locus_tag="IV223_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8712855.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDHJ010000323         1415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.327, whole genome shotgun sequence.
ACCESSION   DACDHJ010000323 DACDHJ010000000
VERSION     DACDHJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.327"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            180..779
                     /locus_tag="IV223_002618"
     CDS             180..779
                     /locus_tag="IV223_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8712856.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            843..>1415
                     /locus_tag="IV223_002619"
     CDS             843..>1415
                     /locus_tag="IV223_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712857.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          492 a          221 c          286 g          416 t
ORIGIN      
        1 aaatatcaaa aatccaatgt gacacaatca gattgtgtct cattcgaggg aatcacacaa
       61 tcccacgtgg ctctaggcag aattgaattt ttgcttgggg ctttttcctt atattgattt
      121 tgaatgagac ttattatatg ttataatgca attaagtatc attggagagg gcgaagaaaa
      181 tgaagacggt agaacctatt cgagacaaaa aaaagatcga tgcgatgaag gcaatcttgg
      241 cttcgggaaa atatggccaa cgaaatttgg ttcttttttc gattgggatc aacaccgcct
      301 atcgcatttc tgatttgcgt caattgaagc tggccgacgt gctggaaatt tcacgcggtc
      361 gcgtggtggt caaagatcga ttagcgatga aggaacaaaa aacagctaaa cataattcgg
      421 tctatatttc caacaaattg cgaaaaacga ttttggagta cgttcaacag gaatttcctg
      481 atcaactcct tcagcaagat tttgacggtt atttgttccc tagtcgaaaa gggacgaatg
      541 aaccgctcaa ccgccaaagt ctgtggcgaa tcattcatga tgctggcacg gcaatcggat
      601 tgaaagacat tggcccacat tccatgcgaa aaacgttcgg ttactttctc tataaacagg
      661 ggaccaaaac agaaatcatt cagtccttgt tgaaccattc ttctcagcgc gaaacattgc
      721 ggtatattgg tattacacaa gaagacaaag atacagctgt aaaaagctta gatttataat
      781 ataaaagtta agtttaaaca tggatagttt taaattagta gattgagtgt tgggaggaaa
      841 gaatgagtac attatatatt ataggaaacg gttttgattt aagtcatggt ttaaaaacta
      901 gatacactga tttttcaaaa tgggttgaaa aaagtcatag tgaaatattc gataaaatag
      961 acacaatgat aaatgaaaat aatgaggtga gaagtacagc tgatgataag ataggttggt
     1021 ttaattttga aagtagtttg catgagttaa ttcaaagtcc agtctttgaa gattatgtag
     1081 aagagtctaa ggattatctc ataggttatg gacatgaaga ttggtcagat tctgcccatc
     1141 atgattttca aaatatggtt tatgattatg ttgtttctct agaagaatta caacaacttt
     1201 tcaaggaatg gatacgcaaa gaaatacagc cgacaataga acataaaatt ccaaaatacc
     1261 aattttcgtc cgatgtacta ttctttacat tcaattatac agatgttttg gaaattctat
     1321 acaagcaacc gaaagaaaac atatatcata ttcatggcag ttatgacgat ataataatcg
     1381 gccactccga gaattttgac aactttatag gagat
//
LOCUS       DACDHJ010000324         1392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.328, whole genome shotgun sequence.
ACCESSION   DACDHJ010000324 DACDHJ010000000
VERSION     DACDHJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.328"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(873..1262)
                     /locus_tag="IV223_002620"
     CDS             complement(873..1262)
                     /locus_tag="IV223_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8712858.1"
                     /translation="MGDIDLENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYK
                     KSLIENNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLL
                     PLVGIITTGICLFKKGKDNKTDSSQVS"
BASE COUNT          501 a          253 c          153 g          485 t
ORIGIN      
        1 actacctcgg aaatagatat cctacataat acatcattat tgtcagtata gattttaaaa
       61 aacattagat ttgagccttc tttggacagc gaggttagta atttaggaaa atcaattgca
      121 tcttcatatc ttgcataaca tgttctctta agattcctta ctttaacttc cgatattcct
      181 agctctctag acaaggttct atctgagtaa tcactattac tatttatctt atgttttaga
      241 tacgagtaaa agagcatgct ttcaatttca ctttttgttg ctaatccaaa attacgatta
      301 aaaaaaagcg gatacatttt ttccccaatc tctttaaaca gattgtcttt gctttctata
      361 ttattttctt ccataggtat agaattacac cttcctttat tttgatgtta cctattacta
      421 gggaaaacca ttcttaacat caattttagc atactcttac atgaaaaaaa attttaatat
      481 atctatcaac aatctacttt aaaaacttat ttcattgaat agctagtgaa atataactag
      541 cttgtttcat acttacaaaa tcattcttgt acgaaaaagt accctacctt ttaaatttat
      601 catgtattat ccgttagtaa actttactac tgcatatgcc caaagtgacg atggttatta
      661 ggacaacttt gcaaaaagta aagtccctaa acatttatgg aataaatatc aacaagctaa
      721 agcaaaacaa ggtgcttgta ttatgaaagg aatttggtct gcagtagcct tccataaact
      781 ctcatggtca gctgcgattc aagctgctta caatgcaagt tcttgtcatt catttaatag
      841 acaatactct taataaataa aacagatagc aatcaagaaa cttgactgct atctgtttta
      901 ttgtcttttc cctttttaaa taaacaaatc cctgttgtga taataccaac taacggtaac
      961 agacttagta aagctacaaa tttcgaccac ccaatatcaa caaaccttct tgcttggaac
     1021 agaaaaccta taataatcat tataaagaac acgatcctaa gaaaattata aaaatcaaat
     1081 ggaccattta tcacgaaata ttttaaataa ttgttttcaa tcagagactt tttgtataca
     1141 aacattgaaa aagtttctat ccctaatcca aacacaaaca gtatacactg ataataccaa
     1201 tattttcttc ttgataatcc cttgtttaaa tcaaaaatat tttccaaatc tatatccccc
     1261 aaaacctact aatgctactt tatgtattta tttacaatac cattgtaatt ggcaaataca
     1321 taaaatagca aataatatcc taaaacagct atgatttttt ccttacaaag tatacatttc
     1381 tgattgaatt ta
//
LOCUS       DACDHJ010000325         1366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.329, whole genome shotgun sequence.
ACCESSION   DACDHJ010000325 DACDHJ010000000
VERSION     DACDHJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.329"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..970)
                     /locus_tag="IV223_002621"
     CDS             complement(<1..970)
                     /locus_tag="IV223_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8712859.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNL"
     gene            complement(989..>1366)
                     /locus_tag="IV223_002622"
     CDS             complement(989..>1366)
                     /locus_tag="IV223_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAP8712860.1"
                     /translation="FSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLEQVVLLADES
                     LEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQATFQ
                     LNDLNKAFLASHIYGKILSAKFE"
BASE COUNT          415 a          273 c          191 g          487 t
ORIGIN      
        1 tcaaattgcc aacattttca ctaaagcgat agtttaataa aaaatctaat aattgttgag
       61 atatcagtat tttctttgct agtctttctt cttctgattt aaaaaatatc ttgattaact
      121 gttttctttc ataaagagaa cgactgtcta atgttggaag tgttagcttt acaggtattc
      181 ttctcaacaa agttgataat agtttatcct caggtggttc tgatgttgca aatataatcc
      241 agcaacttga tgtgtaccag ttttcattat cacccatccg atgaaatttc ctcgtatcca
      301 taaactgaaa aagtttctcc tgacattcat tgctcaatgc atgaatttca tctaaaaata
      361 agactccatt attagctaat tctgctaaac cctttcttcc atcttcggct cctgtatatg
      421 caccttttac atgcccaaaa aaattggaca agaaaagttc tggattattg gcatattcac
      481 tacaattaac tgaaatgaaa gatgccttct cttctatata attattggcc aacaaatcct
      541 tatacacata atttgctaaa tagctttttc cagttccagt ctcgccatga agtaaaatgg
      601 gtaagccatt tggcggatag tagattgctg ctttaatttg agcaattaca tttcgcagac
      661 ttccttgact tccgataacc ctttctattc caaccctaaa agagctatca taccattttt
      721 ccgcttcctc tgaagagcta aaaatttgtt gatttggaac tttctccggt aaagaaaaga
      781 agattactgg acgagtattg atttttccaa attctccatc ttttacgaat tcattcaaat
      841 attgagagac aatattacga ctaacattta atttttcaga tattttctgt gctgtattgt
      901 tcccccattt atttttaatc tcagagttct ctatgacata attgacaatg cgttctttta
      961 attcttccat cacagcacct cctcgacact attcgaattt tgccgacagt atttttccat
     1021 aaatatgact tgctaaaaag gctttgttta gatcattcaa ttggaacgtt gcttgttttg
     1081 cttgatccgc taagacgttg aaaagatact ctggcgaaat cgcatacccc aaaatatcaa
     1141 cgattgttgc aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca
     1201 tcgtttgttt gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt
     1261 gttccaacaa acggtcggat aaatatttat agaaaatcag ccccaataaa taatttttat
     1321 attctgacgc atccatttta ctacggagat tatctgccgc actaaa
//
LOCUS       DACDHJ010000326         1362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.330, whole genome shotgun sequence.
ACCESSION   DACDHJ010000326 DACDHJ010000000
VERSION     DACDHJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.330"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1362
                     /locus_tag="IV223_002623"
     CDS             82..>1362
                     /locus_tag="IV223_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8712861.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DACDHJ010000327         1347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.331, whole genome shotgun sequence.
ACCESSION   DACDHJ010000327 DACDHJ010000000
VERSION     DACDHJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.331"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1347
                     /locus_tag="IV223_002624"
     CDS             <1..>1347
                     /locus_tag="IV223_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8712862.1"
                     /translation="YPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKNTQLPSQRE
                     LADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEVTEHIIDFG
                     LVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKTQGVIPSLE
                     SLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPIDFDEQGMR
                     PDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVIEDDIHSFL
                     TTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIEQAIFNINV
                     KTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRVPYYRILPV
                     DPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILASGLSIVK"
BASE COUNT          441 a          255 c          216 g          435 t
ORIGIN      
        1 attacccgtt aacttggcga ccagacaaac aaaaattgaa gcgtccttat tatcgatcgt
       61 tgattcaaca attagaacaa gatattttaa agaataaatt acagaaaaac actcagttac
      121 cctcccaaag agagctagct gattatttag atttaaattt tactaccgtt ggcaaggctt
      181 ataaatatgg aattgagaaa ggtttgctgt atacgaatat tggaagtggc acatttattt
      241 ctcccaatgt ctttgagtcc atcaccatct ctaccaatga agtaaccgaa catatcattg
      301 attttggtct ggttagttct tttgaagaat gtaataaatt cgttgtcccg tttattcaat
      361 caactagtaa taacccaaat cttgttggtt tgcttaatta ccaagatccc aaaggcacaa
      421 attttcaatt gactactgca gcaaattggt taaaaactca aggagtcatt ccgtccttag
      481 aatctctatc tattgtttcc ggtgttcaaa atggattggc tatcacactt tctgcacttt
      541 tctctccagg tgatagaata gcaacagacc gatatacata cgctaatttc attgaattgg
      601 ctcagttata tcatttagag atcgttccaa ttgattttga cgagcaagga atgcgacctg
      661 atatattgat tcaggaatgt agaaaaaaga agattcatgg tgtctttctt atgccttctt
      721 gtaataatcc tatagggttt caaatctctc acaaaagacg agagcaatta gccgaagtga
      781 ttgaaaaaga aggtctatgg gttattgaag atgatatcca ttcattttta acaacttact
      841 atcaacatac gatcattcct cctttccaaa atttattacc taaccaaaca atctacttag
      901 ctggaatgac aaaatttatt tgttctggat tgcgagttgc ttatcttgta gttccagtac
      961 atgtccaaag caagatagaa caagcaattt ttaatataaa tgtgaaaact tctggattag
     1021 atgtggaagt catcactcaa atcctccatt caaatgaagc aaataggatc ttagaaacta
     1081 aattaagtct tacaaaaaga tccaatcagc tttttgattc tcttttcaaa gtagaaaaac
     1141 caacgaatcg tgtcccttac tatcgaattc ttccagttga tcctgagatt gaccctaaga
     1201 taattgagaa tgactttctt gctaacggta tccgacttta tcattccaga cgctttacta
     1261 cacgtgcgca accagatgca ttcgttcgaa tttccctatc ctctaataac cttgaaattt
     1321 tagcttctgg actaagtatc gtaaaag
//
LOCUS       DACDHJ010000328         1342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.332, whole genome shotgun sequence.
ACCESSION   DACDHJ010000328 DACDHJ010000000
VERSION     DACDHJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.332"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            266..>1342
                     /locus_tag="IV223_002625"
     CDS             266..>1342
                     /locus_tag="IV223_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712863.1"
                     /translation="MIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQ
                     MNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEK
                     KEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKK
                     TMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGL
                     YDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYL
                     GTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVY
                     SSFKRFAPNKLKDSRYVTKKEINKK"
BASE COUNT          468 a          200 c          241 g          433 t
ORIGIN      
        1 tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt tatatacttt
       61 tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag caatcgctct
      121 tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata gagaaaaaaa
      181 agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct taactacact
      241 attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa gcaagcaaat
      301 aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt tgacttgtgc
      361 attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag aaatacacag
      421 tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta tcattcaagc
      481 gttaaataac gatattacaa catacactat ggggttatta ggattggttg cgttgatttt
      541 tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg ttggcgtagt
      601 ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa aaacaccgat
      661 aagcttactc cagtttcctt ggcgtatctt aggtatccaa atcttttttt cttcaatgat
      721 cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt taatcattgg
      781 cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt acgcaaaagt
      841 tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac gttatacaac
      901 atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag aaaggcctca
      961 cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt ataaagctgc
     1021 agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga cagtaccagt
     1081 atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg actcttataa
     1141 aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag aaattacaac
     1201 aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt attgtttagt
     1261 ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc gatatgtgac
     1321 taaaaaggag ataaacaaga ag
//
LOCUS       DACDHJ010000329         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.333, whole genome shotgun sequence.
ACCESSION   DACDHJ010000329 DACDHJ010000000
VERSION     DACDHJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.333"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(128..460)
                     /locus_tag="IV223_002626"
     CDS             complement(128..460)
                     /locus_tag="IV223_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712864.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(512..>1324)
                     /locus_tag="IV223_002627"
     CDS             complement(512..>1324)
                     /locus_tag="IV223_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712865.1"
                     /translation="KLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNI
                     NSENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRL
                     NQFKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREA
                     APEPIQRVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
BASE COUNT          346 a          256 c          219 g          503 t
ORIGIN      
        1 tagtaactaa gactccctct tctgtacagg atttataagg aaataaatcc aagatagatc
       61 gttcttctgg ttttccaaac tgaaaaatgg atttcttatc aactgaattt tgtcctttga
      121 ctcgcattta ttcatcctcc ttatacgtac catgataatt tcggttagga tctaacgcat
      181 gataatggtt cttgtccgta aaaaaaatag actcgataat cgcaaaaatt tgtcgttgac
      241 ctgggttatt atagggtcgc caaacaatca ctagagctat cgcaatccat aagccataat
      301 tcaagagtgc aaaaaagagt ggtaaatgga caaggttatt gactattttc gcccaaaaaa
      361 agcaaccacc aagcgttaat agatccaaaa agaaaaaggc aaaaaacttc cattccccac
      421 taaatcttgg aggatttttc atgttaatat atttatccat aaatgatttc tccttttcat
      481 acatagaaaa aaggcctaac ttgtaggcct tttatggttt gatatcctgt ttgatatccc
      541 tcaatagttg ttctttcaat tcattgttcc tgtttttagt ttctcttgtc acatttattt
      601 ttgttggtgc agtaaatgcc acatttgctg gtttgtaacc tgtgtgaact ctttgaatag
      661 gttctggtgc agcttctctt gtcacaacat tttgaacgga cgcattccct gtctccccac
      721 tttcagataa attgtaagaa acagttgtca gatttccatt ttcaggcaaa cgacttggtt
      781 tcatttgaac attacctgca gttgattggt gaacaaagct ttgatcattc tccttgaatt
      841 gattcaaacg actatctttc attggtaaat tttgtgcctt aggtaaacca attttcgttg
      901 atttttcagt tccaacttcg accccattat catttggcag atcgatggaa tcatcatccg
      961 tttgtacttc ttgatttcgt tttggtaaat ctgaaatgcg cttttcattt tcagaattaa
     1021 tgttagattt ataacgatat tgttctggat ctgtttttgg taattgacca ttttgtcctt
     1081 cttctgaatt ttcgttagaa tgcaactcat cttggtgatc tttggctgaa ggaatcgctg
     1141 caccttcttg attttctttt tcggttgttg tattagcttt tactgctgca ttgacttgtc
     1201 cttcaccata cgcatctttt agtccttctt tgacttctga agctttttct ttttgttttt
     1261 ctagtgtttc ggatggattc ttcacagctt cagccaattc tttagctctc gtgccgtcta
     1321 attt
//
LOCUS       DACDHJ010000330         1321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.334, whole genome shotgun sequence.
ACCESSION   DACDHJ010000330 DACDHJ010000000
VERSION     DACDHJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.334"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(184..1209)
                     /locus_tag="IV223_002628"
     CDS             complement(184..1209)
                     /locus_tag="IV223_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8712866.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          379 a          223 c          265 g          454 t
ORIGIN      
        1 tttcttccct gtggaaaaaa tagtttaaca caaacattat agttatccac agctatctaa
       61 aacagatgga aaataagaaa aattcgctaa aataccggga gggggtaacg agggatgagt
      121 gattgaaaaa agaacagagt aactgactta tcctcagtgt ttctgttctt ttataattct
      181 gttctacatt tcttgttgtt ggatcgaatc tgtgagagta atctcggttg aaagaatgat
      241 tcttttttct tcagggtatt ggtggtggat tagttggaat aaaagttctg tggctttgat
      301 tcctaactga tttgtcggaa tgtctatccg agtcaactgt ggcgaaaata gggaggacaa
      361 taagggatca ctgaatcctg cgatcgaaag atcatcaggt atctgctttc cagattgttc
      421 tgccactttg taaatactca atgattttaa tgcatcggta gaaaaaatcg cagttggtct
      481 ttctcgtagc ttcattaaat cattcattgc tgccatgcta tcttctaagc gaaagccgcc
      541 gtctataatc cattcattac ggatatctag gtggtgatca aataagcatt gacggtaacc
      601 attgagacga tcattggata cataaacttc tgtcggagca tggagacagc cgatatgacg
      661 gtgtccattc ttgatcagtg tttccaccaa atcgtaacta tcctggaaat tatccgtgtc
      721 cactgagtag atgttcgtgt agtcgtttcc gacttttccg attaccacaa taggaatatt
      781 gtattgatcc aattcaaaaa gaagtgtttc atccatcgta gagcttaaaa taataattcc
      841 tcggatcatt ttttcttgga tctttttcgc gcatttttct agctcttctt ctggtgtttt
      901 tgccgtttgt agaatcaagt cgaagttatt ttgttcagaa acggcagaaa tggattgaat
      961 aacatctgaa aaaaaaggat tgccataggt cgtatgagaa gtacgatgag ataatgctag
     1021 aatcgcgtca aaaccatttc ctgacagtcc tcttgcaatt ttgctcggtt cataattcaa
     1081 ttctttgatt gcttgaagta cacttgcttt tgctttatca gagatatttg gctggttatt
     1141 taatactctg gagacagttg attttgagac attagctttt ttcgcaatgt cataaatagt
     1201 aggtttcaat tttatatgca cctccttttt gggagcgttt tagaataatt ccatagtaca
     1261 atagacattc ttttttgtct atgattttga aaatttagga atagcttgca tgatcatgag
     1321 a
//
LOCUS       DACDHJ010000331         1317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.335, whole genome shotgun sequence.
ACCESSION   DACDHJ010000331 DACDHJ010000000
VERSION     DACDHJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.335"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..1317
                     /locus_tag="IV223_002629"
                     /pseudo
     CDS             1..1317
                     /locus_tag="IV223_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
BASE COUNT          462 a          216 c          210 g          429 t
ORIGIN      
        1 tcaaattttt tactatacat caaagaatta gaacaagact ttgagcgtct caatctaaca
       61 gaactgcata ttgataagca aaaacctttt ttaaagttga attttgaagg aattgatccg
      121 gcttactgct attatcgtct atttggaaga tactgcaacg aatcagtcag ttatcagatc
      181 ttgacttctt tgttcagctg tcagacaaat tcgattatca gtttcagtca gcaaacaaac
      241 tatagtgcct cttatttgta tacgaaaatg aaaaagatta atgcattttt agcatagtac
      301 ggtttgtcta tctccttttc taataatggc agaaagtctt tttctggaaa agaggtacaa
      361 attcaatatg cagcattaga tatttattgg aacatctttt caagtactgc tacagaattt
      421 tatgatgaag atccgcaagt agtggctttt atgatgaata ctttttttaa aaaagaagta
      481 cttgaccgac taaatagcgg tatgatagat aaattatatt ttctattaaa aatatgtaaa
      541 gagaattttc ctgcaacttc tgtgaaagac gtacaaaaag aattcacaga gtatgaatat
      601 attgactttt ttattgatct ctctgtagat attttgcgac ctaatctgcc aatttgtcgc
      661 gaacaacgca tcttagtgaa tattttagct cgactttcta tcacgaaaat cgaatcggaa
      721 gaaacaagct tggagcaata caaactttta aaaaaagcaa acgttcctca tgttctttat
      781 tcaagagaaa tggtggagtc cctttgtact gtatttgatt tgtttatacc tgaaaaagaa
      841 aagattcttt actcattggc attcgcacga aataaacttt ataatggatt tcttaacttc
      901 aatcaaccaa atacgcccct tcctactttc atgctttatc aagaacatag cgtattgaaa
      961 gaagtccaaa ccgccattga acaattttat atggatttca gagaacaaaa tcaacacttg
     1021 ttaccagtta ttttagaaaa ggaaaatgtg caatggatgg ttgaagacct agtacatttg
     1081 tatgatcgct acaaaaaaca gccgcgaatt gtgattggtg tgaactatac gcgtgatttt
     1141 tatatcagta aagatctgat cgttaagata gaacaaattt tttcaaatga atcgatcaaa
     1201 atacaaaatg attttatgga gaactgcaac atcgttatca gcgactgccc tttgaaacaa
     1261 ttgccttctc atatcaaaaa aatctatatt ttaaaaggaa tcatcacacc agaagac
//
LOCUS       DACDHJ010000332         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.336, whole genome shotgun sequence.
ACCESSION   DACDHJ010000332 DACDHJ010000000
VERSION     DACDHJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.336"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            129..>1311
                     /locus_tag="IV223_002630"
     CDS             129..>1311
                     /locus_tag="IV223_002630"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8712867.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          411 a          262 c          236 g          402 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gctccttatt tctcactagt taaagagtgt tttccaaatg
      841 cacaagtgat cgtcgatcgg tttcacattg ttcaacatct gaaccgcttc ttcgatgcgg
      901 ttcgaaaacg tgtgatgaaa acacttgatc aaaaagaccc aatacaagct aaacagtatc
      961 gccaattgaa atccttattc aaattacttt taaaatcaga ggatcgttta gattacaata
     1021 cactaacgaa aagacgcaat ttcaattgga gactattgac tgaaacagaa gtagttgacc
     1081 gccttctaaa gatttctcct gaacttaaaa cagcttatcg ttactaccaa gacctgctta
     1141 ctgcgtatca ggaaaaagat ccagatactt ttttccagct gcttcgagca atgcctggag
     1201 aagttccatt ggaactgcac catatcaaaa aagccttttt caaatacgag aaagggattc
     1261 ggctggcatt actgaagaag tattcgaatg cacgactgga aaatctccat a
//
LOCUS       DACDHJ010000333         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.337, whole genome shotgun sequence.
ACCESSION   DACDHJ010000333 DACDHJ010000000
VERSION     DACDHJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.337"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1288
                     /locus_tag="IV223_002631"
     CDS             82..>1288
                     /locus_tag="IV223_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8712868.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIK"
BASE COUNT          433 a          245 c          248 g          362 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaaga
//
LOCUS       DACDHJ010000334         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.338, whole genome shotgun sequence.
ACCESSION   DACDHJ010000334 DACDHJ010000000
VERSION     DACDHJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.338"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            34..531
                     /locus_tag="IV223_002632"
     CDS             34..531
                     /locus_tag="IV223_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712869.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRIYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(528..1262)
                     /locus_tag="IV223_002633"
     CDS             complement(528..1262)
                     /locus_tag="IV223_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712870.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKGKRIMVPLQSKKTSSASKVRTFF"
BASE COUNT          447 a          216 c          225 g          400 t
ORIGIN      
        1 tttatagtat actatgtcaa aaggaggagt tatatgaaca atcaaatcac actagcgtat
       61 ttagattatg atgatctaga tgctttacaa gttgtaagtg ttgtcagttt ttatgaatcg
      121 tttatcgatg gcgcagatcc acttgatatg caaggatatt tacagacaca attgacaact
      181 gaaatattgg cagtagaatt ggcacagtca accagtaaat ttattggtat taaggatcat
      241 caaatactca ttggttatat gaaagtcaat gatgaaaaag atgctataga gattcaacgg
      301 atctatctgc tcaaagatta tcaaaacaaa gggttagggc aacggttact cgatgaagcg
      361 aatcgttatg ctaaaacgaa acaaaaacgt tatttacgtt tgactgttta tgaaaaaaat
      421 catgcaggta ttcgttttta tgaacgtcat ggatttaaaa aaatcgggat caaacatttc
      481 cctttaggaa aacaagatcg tatttgtccg attttagaaa aagaaatcta aaaaaacgtc
      541 ctcacttttg atgctgagga cgttttttta gactgtaatg gaaccataat tcgctttcct
      601 ttttgataaa tcgctaattc cgtcacacct tttgctttta acagagcaaa aagatcatcg
      661 aaatggaaac ggaaatgttc aaggtaatgc ccatctgatc caacactaaa caaggtacca
      721 ccacgtgaaa gatataaatc gatgacatat tcatataacg ctaaattgtc atacaagtaa
      781 gcgctcttag catttaattc gaatgccaat ttatttttga ttacttgatc tagaatcggt
      841 aaaaactgcg cttcatattg tttaaattca gcaacggata gaccaaatac ccgaaaacca
      901 tagtcaaaat gagcaaagac atctgcttct ataacaacta gtgcttctga aagttgtgca
      961 aaatactgag gaatcacaat agcaggatcc atatctttga cttcgtcatc taaataatca
     1021 aattgaccat tatgatgcac actgagtaat tttaaatcgt aatctttatc ggctaaatag
     1081 tcgatgatcc gatccttttc agaagcgatg taaccgattt cgatgccttt taaaacacga
     1141 ttgccaaaac gcttatttaa gctatcgatt gtttgactgt acatttcata atcggggata
     1201 tcatcgactt tagtaacagg attggctaag tcaaaatgtt cagtatattt tgatgaacgc
     1261 atatgtagtc ctcatttctt gggagatt
//
LOCUS       DACDHJ010000335         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.339, whole genome shotgun sequence.
ACCESSION   DACDHJ010000335 DACDHJ010000000
VERSION     DACDHJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.339"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            201..>1265
                     /gene="scm"
                     /locus_tag="IV223_002634"
     CDS             201..>1265
                     /gene="scm"
                     /locus_tag="IV223_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8712871.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          456 a          216 c          234 g          359 t
ORIGIN      
        1 acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa
       61 ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa
      121 tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg
      181 aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt
      241 tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc
      301 ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat
      361 tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa
      421 cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc
      481 aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag
      541 gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg
      601 gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag
      661 atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag
      721 caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca
      781 tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata
      841 tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag
      901 tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat
      961 acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt
     1021 ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc
     1081 ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga
     1141 ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta
     1201 cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt
     1261 catca
//
LOCUS       DACDHJ010000336         1260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.340, whole genome shotgun sequence.
ACCESSION   DACDHJ010000336 DACDHJ010000000
VERSION     DACDHJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.340"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..666
                     /locus_tag="IV223_002635"
     CDS             <1..666
                     /locus_tag="IV223_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8712872.1"
                     /translation="VQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVD
                     CFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMG
                     AKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKE
                     VSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEE
                     DVE"
     gene            663..>1260
                     /gene="neuC"
                     /locus_tag="IV223_002636"
     CDS             663..>1260
                     /gene="neuC"
                     /locus_tag="IV223_002636"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8712873.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFE"
BASE COUNT          428 a          163 c          268 g          401 t
ORIGIN      
        1 gtacaaggat ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata
       61 gatatgtatg ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt
      121 gaggctgtag attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat
      181 ccggctagtt ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg
      241 atacccgttg ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg
      301 atgggtgcta aggtaattga gaaacatttt accttggata aggaactacc tggtccagat
      361 cataaagctt ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact
      421 gaaatatttt taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa
      481 gtatcaagaa aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag
      541 aaatctctta caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat
      601 attggcaaaa aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt
      661 gaatgaaaaa aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg
      721 ttgctgagaa tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt
      781 tatcccataa atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg
      841 gagaagttca aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat
      901 tagagataaa tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac
      961 taggggatag aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa
     1021 cagcacatat tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca
     1081 taacgaaaat agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa
     1141 aaatggggga agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt
     1201 taaataatag attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa
//
LOCUS       DACDHJ010000337         1222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.341, whole genome shotgun sequence.
ACCESSION   DACDHJ010000337 DACDHJ010000000
VERSION     DACDHJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.341"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..1166
                     /locus_tag="IV223_002637"
     CDS             21..1166
                     /locus_tag="IV223_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8712874.1"
                     /translation="MEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGF
                     NSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQK
                     GITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIP
                     LKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVV
                     TDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEE
                     ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKY
                     TKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          441 a          183 c          205 g          393 t
ORIGIN      
        1 acgattttac tacagaaatt atggaaatac taatcaataa aggtgatttg gatgatttgt
       61 ttcgtcgtca cctagaattg gctattaacg ctttattaca agctgaatta acggtatttc
      121 ttgactatga aaagtatgat agagctggat ttaattcagg taattcccgt aatgggaatt
      181 attcgcgctc ttttaaaaca gagtacggtg aattgaattt agtaatacct agagatagga
      241 atggagactt ttctcaacaa actttacctg cttataaaag aaccaatgac tctttagaaa
      301 ccactattat tcagctagtt caaaaaggaa ttactatgtc cgaaatctct gatctaatcg
      361 aaaaaatgta tggtcattat tacaaaccac aaactatttc aaatatgagt aaaattgtgt
      421 ctgaagatgt ttcggcattt aaagaaagaa ctttagaagc taaatactca gtcattttta
      481 tggatgctac tcatattccc ttaaaaagac aaaccgtatc aaaagaagct gtctatatag
      541 taattggtat caggttggat ggaactaaag aggttctagg atttactata gctccaactg
      601 aatctgctta tgtttggaaa gagattcttc aagatctaaa agaccgtggt ttagaagagg
      661 ttttgttagt cgtaacagat ggtttaagtg gtattgacga tagtattcat agtgtttatc
      721 caaatgctca atttcaacaa tgttgtgtcc atatatctag aaatatcgct cataaggttc
      781 gtgttagtga tcgacaagaa atctgtagcg atttcaaatt agtttaccaa gcaagttcaa
      841 aagaagaagc aaataatcaa ataaggttta tgatagataa atggaaaaag cagtatccac
      901 gagtggttaa attgctcatg aatcctgcta tattaacctt ttataatttc cctccgtcaa
      961 tcagaagaac tatttattca actaatttga ttgaaggttt taacaaacaa ttaaagaaat
     1021 ataccaaaag aaaagagcaa tttcctaatg aagaatctct ggagagattt ctagtatctc
     1081 aattcaacaa ctataatcaa aagtttctat gccgagttca taaaggtttt aaagaaatac
     1141 atgatacatt agaatcaatg ttttaacttg atcaacagaa tgggcttttc catttacaca
     1201 taattattga cacaaccctt tt
//
LOCUS       DACDHJ010000338         1217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.342, whole genome shotgun sequence.
ACCESSION   DACDHJ010000338 DACDHJ010000000
VERSION     DACDHJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.342"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1072
                     /locus_tag="IV223_002638"
     CDS             <1..1072
                     /locus_tag="IV223_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8712875.1"
                     /translation="GWQFYNNEHSQIVAHAQSAQKKLILTTNEQQLVEKIDQLLRKND
                     YIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKALTTLLILKQVERGH
                     LSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFT
                     DYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHAL
                     SYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSL
                     WRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAIIMESNIKADKTIKL
                     PTADLRNQIYDLMEGTTKLANS"
BASE COUNT          440 a          229 c          201 g          347 t
ORIGIN      
        1 tggatggcaa ttttacaata atgaacactc tcagatagtt gcccatgctc aatcagctca
       61 aaaaaagttg atactgacta ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt
      121 aagaaaaaat gattatattg gctcaattta cgtaaagaaa aataatcgca tcatcatgga
      181 aaaaggctat ggctatgcga ataaactcac gcaacaacca aatgatcctt ctctttatta
      241 tcagattggt tcggttcaaa aagcattgac tactcttttg attttaaaac aagtcgagcg
      301 tgggcacctt tctttagata ctaagctggc tcaattctat ccacaaattg ctggcagcca
      361 gcaaattact atccgtgact tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc
      421 aaccataccg atgactgatg aagaagtcgt tcaatttgcg attcagcact taaaatttac
      481 agattatcag acctctcatt atgattcatt gaactttacc atattgactg gaattttgat
      541 caaattaaca aatcaaccgt atgaaaaatt attgaccgat gatatcatcg aacctttagg
      601 attacagcat acaaaatttt atgaccaagt caaaaattca tccaatcatg cactatccta
      661 tcaaatgtct aaagaggatg attactttaa accactgaca gaatctgaaa cagagatcca
      721 taacgaatta gggactggta atatcagtat gtccgtctat gatttaaata cgctgtttac
      781 acaagtttta tctggaaaac tagtttccaa agacttactc ttctcattat ggcgtgaaaa
      841 tgcgaatgga catccatata gtggtggggt atatagcgga gataattata tcctgtcgca
      901 aggaaatatc gactcttttg atacatctgc tatatttagc aaagattcac aggatgctat
      961 catcatggaa agcaatataa aagctgacaa aactatcaaa ctgcctactg ctgatctaag
     1021 aaaccaaatc tatgacctga tggaaggaac tactaagttg gcaaatagct gatatgatga
     1081 aaagatagca aatactactt ctttgaatag catttgctta aaaaaagtac tcgactaaac
     1141 gtaggaaaga gtgtgggaca aatctaaaaa tcaatttttg ctacagactt taattccaaa
     1201 taaacagaac agaaaga
//
LOCUS       DACDHJ010000339         1204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.343, whole genome shotgun sequence.
ACCESSION   DACDHJ010000339 DACDHJ010000000
VERSION     DACDHJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.343"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..650)
                     /locus_tag="IV223_002639"
     CDS             complement(<1..650)
                     /locus_tag="IV223_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8712876.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..>1204
                     /locus_tag="IV223_002640"
     CDS             975..>1204
                     /locus_tag="IV223_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8712877.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLT"
BASE COUNT          393 a          201 c          215 g          395 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctat
//
LOCUS       DACDHJ010000340         1185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.344, whole genome shotgun sequence.
ACCESSION   DACDHJ010000340 DACDHJ010000000
VERSION     DACDHJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.344"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            55..927
                     /locus_tag="IV223_002641"
     CDS             55..927
                     /locus_tag="IV223_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8712878.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          343 a          223 c          283 g          336 t
ORIGIN      
        1 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
       61 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
      121 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
      181 attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg
      241 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaattg
      301 gcatggcaaa attttgattt catcggtacc atgaaaaaag aatttccgat cccgatttct
      361 tggacaacag atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag tggcaaagga
      421 ttgagcagtg tcgtttacta tacgatcggt acgggtgttg gcggcggtgc cttacaagaa
      481 gggcgcttta tcgaagggtt tagtcatcca gaaatggggc atatgctcgt cgtccctcat
      541 ccaaaagatg actttgctgg cagctgtcca tttcatggca actgtttaga agggatggcc
      601 gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac aagaggttgc agaagatgat
      661 ccttattggg aaatcgaagc ctcctatatt gctcaatgtg cctacaatac aaccttgatg
      721 ttttctccag atgtgatcat ttttggcggt ggtgtgatga aacaacgcca tatgctgcaa
      781 aacgtccacg acgcatttgc aaaattggtc aatgggtatg tcgaaacacc tgagttaagc
      841 aaatatatcg tgaccccagc attagaagac aacgctggaa cgctcggttg tttggcattg
      901 gctcgagaag cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt
      961 agagaaagat acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt
     1021 ttgttttact tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa
     1081 atttcgatag ccgtaggcca cttgtattgt gcaatagttt tttacaaaaa aaagggggtg
     1141 cggatatttt tttggacctt tttaacaaat atatctttgc ttctt
//
LOCUS       DACDHJ010000341         1163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.345, whole genome shotgun sequence.
ACCESSION   DACDHJ010000341 DACDHJ010000000
VERSION     DACDHJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.345"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..424)
                     /locus_tag="IV223_002642"
     CDS             complement(26..424)
                     /locus_tag="IV223_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8712879.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(421..657)
                     /locus_tag="IV223_002643"
     CDS             complement(421..657)
                     /locus_tag="IV223_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8712880.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(817..1134)
                     /locus_tag="IV223_002644"
     CDS             complement(817..1134)
                     /locus_tag="IV223_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712881.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
BASE COUNT          368 a          207 c          178 g          410 t
ORIGIN      
        1 ttgagaaata aagtttttaa ttcgttcatt ttattgcggt ttgtcgtaaa taactggtta
       61 tcttggactt tagtgaatcg aaatccaatg aactagtggt aatcattatg ataaaatcaa
      121 cagcttcttg acgagaaaaa gcaactggac aattattcaa ttgaagaaac aacaacatgg
      181 caacaagagc agttcgttta ttgccattat gaaaaggatg ttttttagct aaattaattg
      241 ctaaaatcgc cgctttttca tagatttcag gatagagttc ttgattgaat actgcttgtg
      301 aaggctgttt gacggccata tctaatgcat ttgcatcttt tacaccgatc atttcgcctg
      361 gcgagtgacg taaaataact ttcgcgttga ttttgataat ttctttagtc gaaagatacc
      421 tcataaatca accaaatctt ttaacgcgtc ttcatattga ttcatgatcg aatcaactaa
      481 tgaatcaata tcaggttctt cttgtgcttt gatcattcgt acagaaccat cagattgaaa
      541 aacataactg attgcgtcac ctgtctgcac acccatgaat tcttttactt ctggtgggat
      601 agttgttaca tcgctattgc cagattttct aattttatac tttttcattt ttttcatatt
      661 tcgccaccct ctataccatt ctcattctat agtaagtaca ttatatccaa gtatatactt
      721 ttaatcaatg tatatacttt tattttgctt tttcttacga aaaaatgaca aagaaaaaag
      781 tttgtgacat gacttttgtc acaaactctc gtattcttaa ccaaaatatt gatgaaatac
      841 ttgtgtaata actggtagaa aataaataat gatcatagcc gctaccacca ccaacaattg
      901 ttttcgctgt ttctttcgct ttagcatgct tgtcgtttta tgaaaaaaat aagtagcgct
      961 tttagctcct gcagtcaaag tcgataaaat caacgttggt gaatatgctg tttgattgtg
     1021 cgtcaatgac aacaacttcg tcccgtaatc aggatacttt agaaatgctt ggtacacagc
     1081 cacaatcgtg attgctccca taagataatt taaaaatgaa tgcacttttt tcaaataaaa
     1141 aaactccttg ctatctatat aaa
//
LOCUS       DACDHJ010000342         1161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.346, whole genome shotgun sequence.
ACCESSION   DACDHJ010000342 DACDHJ010000000
VERSION     DACDHJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.346"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..997
                     /gene="ccpA"
                     /locus_tag="IV223_002645"
     CDS             <1..997
                     /gene="ccpA"
                     /locus_tag="IV223_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8712882.1"
                     /translation="KQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVIDR
                     LDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSDGN
                     DQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSVNI
                     DYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGLIF
                     EAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSNNS
                     LLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT          399 a          209 c          249 g          304 t
ORIGIN      
        1 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
       61 ttcacgtgtc gtaaatggca atccaaacgt caaaccagca acaagaaaaa aagtcttgga
      121 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
      181 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
      241 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
      301 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
      361 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
      421 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
      481 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
      541 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
      601 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
      661 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
      721 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
      781 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
      841 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
      901 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
      961 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
     1021 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
     1081 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
     1141 aaggggttat gacaaaattt t
//
LOCUS       DACDHJ010000343         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.347, whole genome shotgun sequence.
ACCESSION   DACDHJ010000343 DACDHJ010000000
VERSION     DACDHJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.347"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..118
                     /locus_tag="IV223_002646"
     CDS             <1..118
                     /locus_tag="IV223_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8712883.1"
                     /translation="LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            123..1070
                     /locus_tag="IV223_002647"
     CDS             123..1070
                     /locus_tag="IV223_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8712884.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          371 a          171 c          245 g          369 t
ORIGIN      
        1 tctaatttta gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa
       61 atttttagaa gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa
      121 ggatgagctg ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc
      181 atttaggaga agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa
      241 atcttgatat gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga
      301 tggaagagat catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta
      361 gtgtggcaga ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat
      421 taatgatttt agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt
      481 cttcagcggc tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc
      541 gcccattgac gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt
      601 gtcatttata tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc
      661 aaaatccgaa ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac
      721 aaatcgtagg agaagcaact tcatttacat tatttggtga tggaagccag tctagagact
      781 ttgtttatat agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac
      841 tggggaaaca attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg
      901 caatcaatga aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg
      961 atatccatga ctctctagca gatattacta agatccagtc aattggctat caaccgaaat
     1021 atggtatttt aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa
     1081 gataagatta gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa
     1141 tcaaatctgg gataga
//
LOCUS       DACDHJ010000344         1144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.348, whole genome shotgun sequence.
ACCESSION   DACDHJ010000344 DACDHJ010000000
VERSION     DACDHJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.348"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            17..582
                     /locus_tag="IV223_002648"
                     /pseudo
     CDS             17..582
                     /locus_tag="IV223_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          397 a          146 c          256 g          345 t
ORIGIN      
        1 aggtgatttg cgaataatga gaaatttatc aaatagaaac aaaatattaa tcattattgt
       61 tgttatagct gtgtttcatc tgggaacgaa tgcggtttta agtagaataa tattgggtcc
      121 taagccacca agacctgcga ttacccgagg ggagtttgat tttagattag aatatgaagt
      181 agatggggaa aggattgtga tagaagatac aattgttgca ttatttgatg gattttctgc
      241 agatgcaggt agcatggcat ggtatcgcac gtggcgtttg catttggcga gtgacaagag
      301 ggcacaacaa ttattgttac atgagttaga tgatggtcga aaaatatatt atcattttgg
      361 aggagctgga ttttatatgg ggaagttagt agcagtgtaa ctgtaacaag gaaagatttg
      421 ccaggtcttg gcattcgaaa ctccatattt tatatcgagc gtgggagaga tacatctatt
      481 gggaacagga aagggttaac tcttgaagag ttatataatg actttggaat ccgtcttgtt
      541 agttgggaac acaatccacc aatagaaaat cgctttgaat aatcgaaatc tattaatcca
      601 tttcatcagc cagtgaatgt tgtaatggaa caatttggtt tagtgttcaa aaccattgaa
      661 atactaggct gtgatcagca aaaatgtatc tgaataaaaa agagagcaac tattaaatga
      721 gaggagttag gagttgttag ctttatacga atcaaagtgg caaatacaga tgcgtatgca
      781 tttgtgtttg ccatcttgcg tttggtagac gagataggta taagctaaag aaacaacttg
      841 tttaaaataa acgacctagc tcaacaaacg accatctaat caactaacag catacataaa
      901 agcaactatt tttgaagtag aattgaaatc gggataactt aaaaagtgat tgggatacta
      961 gtgtgaatca ccgtacataa atattactcg ttaagggaat ttgagaaatt ttgtatgaaa
     1021 atttaagaat tagttagtat gcgcaagtca aaaatatgaa taaattattt ggtggagata
     1081 atttgctcag cttgtcataa gaaatgagat aatctggcag atgcaagctt ttggacacca
     1141 ttta
//
LOCUS       DACDHJ010000345         1144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.349, whole genome shotgun sequence.
ACCESSION   DACDHJ010000345 DACDHJ010000000
VERSION     DACDHJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.349"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..1011
                     /locus_tag="IV223_002649"
     CDS             79..1011
                     /locus_tag="IV223_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712885.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
BASE COUNT          409 a          159 c          217 g          359 t
ORIGIN      
        1 ttgctagctt tagtacgtta tgtcaattgg gaaggctatg aaaataaaaa tccgtatgta
       61 gaagaagctt gtaacgagtt gattgattat gtagagactc atttttttaa aagaagatat
      121 tcggaagatg aaaagtcctt tatcatacga ggaatagaga tcgcaatcgg aagaaaggca
      181 tttccccttg gactgaaaga agaagataag aaaagcgcag aaacgaagcc gttatttcaa
      241 ttagtctgtc aaggattgga agagcaaaaa ggaattctag acttacaaga agaagatgtc
      301 tattatattt tttctttgtt caacagtaga aattatacca atgagaacat ggagctgtta
      361 agaaaagatt tcgaagttgt ttataaaaat tttgttctca atgacctatc actgaatgaa
      421 ttgattttac agattattag tatcgctgaa atcgacgatg cagaaaattt tattttcaag
      481 caatcctttt tacaatttgt ccgtacgttg tgggcagatg gacaagtatt tttaccggaa
      541 aggatctatc ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt agaaatattg
      601 gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa
      661 ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg ggagacagga aatagaaatt
      721 tttgttgttt cagaaagttc agtcaaacaa tttttttatc gaacccaatt tcatttgtat
      781 gcggaagaag gcatcgctga aataaatcct attttatttc gttcattaga agaattgcct
      841 gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat
      901 ggattagaaa ctgaaaagac aaagatctat ccaatctcat taaaaggtgc aaacatccat
      961 ttgtatcata ttgctcagga acttcaccta ttaaaaaaac atatttcttg atgagacctt
     1021 aattaggaga ccttctacaa agctttttaa ttgtctgtag aaggttttta tttgagtcct
     1081 gattcgtgcg aagatctact tatataacaa gctttctctg aaattttttt aaaatgctat
     1141 tgac
//
LOCUS       DACDHJ010000346         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.350, whole genome shotgun sequence.
ACCESSION   DACDHJ010000346 DACDHJ010000000
VERSION     DACDHJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.350"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..207
                     /locus_tag="IV223_002650"
     CDS             13..207
                     /locus_tag="IV223_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712886.1"
                     /translation="MIFAAVAAVASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVIL
                     AAASIVVSMFSMFGQTPQGL"
     gene            278..>1134
                     /locus_tag="IV223_002651"
     CDS             278..>1134
                     /locus_tag="IV223_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712887.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLP"
BASE COUNT          421 a          156 c          251 g          306 t
ORIGIN      
        1 aaaaataatc tgatgatttt tgctgctgtt gcggcagtgg cttccattgc tcttggaggt
       61 attttcttct tctttgggcg aagaggagct gaaaacggga agacacacgt aggttatgta
      121 ttgttggggg taggagttat tttagctgcc gcttctattg tagtttcaat gttctcaatg
      181 tttggacaaa ccccacaagg actataaaaa aacaagtaga aggagtaaaa agtacgtggc
      241 tactttttac tccttttttt atggaaggag aatattcatg ttttttacga tttcaggcaa
      301 tgcaaaattg gataaagagt taataagaaa agcaacgaag aaagtgggaa agcgtacttc
      361 ctttttaccg aaaacaatta aatattctgt cattgaaacg gaagaacagc tacatgtgtt
      421 aggacaagtg ttagaacaag agaatcaatc ttctgttaaa ttgactctca cgttacatga
      481 taaagaagga aaaatagaaa aggctggcac agaatacgtt tttggctcac tggattatca
      541 aatttcagaa ggtttttatc ctacattgat tgatttaatc cgcctagata tatttaaaga
      601 tacggatgaa accatgagtg tggaagagtc ttatcgaaac gaaaaaattt tacaagtttt
      661 agaagaagag ttagaaagcg accaaagaaa agtgaatcaa gtgagtgaag tcgaagcaaa
      721 cgaagaagta ccatgggaac gcccaatctt tccaaaggat gaatcaaaag aaaaagagaa
      781 aggaattatt gacgaacaag ctgacatttt gactcataaa tcaatatttg aggaagagaa
      841 cgaggtaatg ggtcaattgg aggtagatcc ctttgaagaa acaatggagg atgaagagac
      901 ggccgaagta gaagttgatc cagttgaagc taatgaagac ccaacatgga ctgaagggga
      961 ttctcaggaa tatattgaac cacttatcag tgaagaagta gagaaaaatg aaacagagat
     1021 acaaaaggca acggtatttg attatatttt ggatcaatat ccgccagagc atgaatggat
     1081 gaaagaaaaa aataaattat ttttaaatga actttattcg aaatatcaat tacc
//
LOCUS       DACDHJ010000347         1133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.351, whole genome shotgun sequence.
ACCESSION   DACDHJ010000347 DACDHJ010000000
VERSION     DACDHJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.351"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..207
                     /locus_tag="IV223_002652"
     CDS             13..207
                     /locus_tag="IV223_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712888.1"
                     /translation="MIFAAAAAVASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVIL
                     AAASIVVSLFGLFGQTPQGL"
     gene            277..>1133
                     /locus_tag="IV223_002653"
     CDS             277..>1133
                     /locus_tag="IV223_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712889.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLP"
BASE COUNT          414 a          155 c          264 g          300 t
ORIGIN      
        1 aaaaacaatc tgatgatttt tgctgctgct gcggccgttg cttccattgt tattgggggt
       61 cttttcttcc tatttggacg aagaggagct gaaaatggga agatgcacgt aggctatgta
      121 ttgatggggg taggagttat tttagcagca gcttctattg tggtttcgtt atttggattg
      181 tttggacaaa cacctcaagg attataagaa agtaaaaaag taggagtaag aagtagtcga
      241 tactttttac tcctttttta tgaaaggaga atactcatgt actttacgat tgcaggcaat
      301 accaaaacag ataaagagtt aataaaaaaa gcaacgaagg aagtaggaaa gcgtacttct
      361 tttttaccga aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta
      421 gggaaagtac tggaacacga aagtcgatct tctgttaagt tgactctcac gttacatgac
      481 acagaaggaa aaatagagaa agctggtaca gaatacgttt ttggcccact ggattatcaa
      541 atttcagaag gtttctatcc tacattgatg gatttaatcc gccttgatgt gtttaaagaa
      601 acggatgaaa ctatgagtgt ggaagagtct tatcgaaacg aaaaactttt acaagtttta
      661 gaagaagagt tagagagcga ccaaagaaaa gtgaatcaag ggagtgaagt cgaagtaaat
      721 gaagaagtac catgggaacg accaatattt ccaaagaagg agtcaaaaga aaaagagaca
      781 gtctatgttg acgaacaagc tgatattccg actcagaaat cgatatttga ggaagagaac
      841 gatgtgatgg gtcaattgga ggtagatccc tttgaagaaa caatggagga tgaagagacg
      901 gccgaagtag aagttgatcc agttgaagcg aatgaagagt caacatggac tgaaggggat
      961 tctcaggaat atattgaacc acttatcagt gaagaagtag aaaaaaacga aatagagata
     1021 caaaaggcaa ctgtatttga ttatattttg gatcaatatc cgccagagca tgaatggatg
     1081 aaagaaaaaa acaaattatt tttaaatgag ctttattcga aatatcaatt acc
//
LOCUS       DACDHJ010000348         1132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.352, whole genome shotgun sequence.
ACCESSION   DACDHJ010000348 DACDHJ010000000
VERSION     DACDHJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.352"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..478
                     /locus_tag="IV223_002654"
                     /pseudo
     CDS             1..478
                     /locus_tag="IV223_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1007..>1132)
                     /locus_tag="IV223_002655"
     CDS             complement(1007..>1132)
                     /locus_tag="IV223_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP8712890.1"
                     /translation="QNFIQYTDAVEPLLQNVEKYHKIYQQCRKIYPATKEICHDL"
BASE COUNT          395 a          218 c          199 g          320 t
ORIGIN      
        1 tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt
       61 ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta
      121 taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat
      181 agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat
      241 aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc
      301 tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta tcgaacgctt
      361 caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca
      421 taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga
      481 gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg cctatcttcc
      541 cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga
      601 ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc
      661 agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca
      721 tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag actttcggga
      781 aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa
      841 agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa
      901 tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa
      961 cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac
     1021 aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta tgatactttt
     1081 ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc tg
//
LOCUS       DACDHJ010000349         1132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.353, whole genome shotgun sequence.
ACCESSION   DACDHJ010000349 DACDHJ010000000
VERSION     DACDHJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.353"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..779)
                     /locus_tag="IV223_002656"
     CDS             complement(45..779)
                     /locus_tag="IV223_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8712891.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          393 a          219 c          205 g          315 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca tt
//
LOCUS       DACDHJ010000350         1117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.354, whole genome shotgun sequence.
ACCESSION   DACDHJ010000350 DACDHJ010000000
VERSION     DACDHJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.354"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            378..1025
                     /locus_tag="IV223_002657"
     CDS             378..1025
                     /locus_tag="IV223_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8712892.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
BASE COUNT          376 a          197 c          210 g          334 t
ORIGIN      
        1 taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag aagtgggcag
       61 cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg aaatttcggc
      121 ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga aaaagctgcc
      181 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta cgtttggttg
      241 agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac acccttttcc
      301 ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat gtgaaattat
      361 taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca actgcgaaaa
      421 gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata agtagaatca
      481 aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc agagacttct
      541 cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt cttatcgatg
      601 tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta ggatatggaa
      661 atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta aatatcgtgt
      721 gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa atgatctatc
      781 cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca atctctacgg
      841 taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat gtaaccgcta
      901 tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt caatacattg
      961 atttgacaac cgagttgcag acgcttattt actttgatga gaattactca gaagtttttt
     1021 cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat tctttcaaaa
     1081 attttttagt actaaatgaa gtggtttttt cacgacc
//
LOCUS       DACDHJ010000351         1102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.355, whole genome shotgun sequence.
ACCESSION   DACDHJ010000351 DACDHJ010000000
VERSION     DACDHJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.355"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(172..429)
                     /locus_tag="IV223_002658"
     CDS             complement(172..429)
                     /locus_tag="IV223_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8712893.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(554..886)
                     /locus_tag="IV223_002659"
     CDS             complement(554..886)
                     /locus_tag="IV223_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712894.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT          344 a          183 c          158 g          417 t
ORIGIN      
        1 agttttagaa aaagcaaaaa aataaagaaa cttattcttg ataacaagct acgtgacgta
       61 tatccctaac acaaaaatag ccatatcctt tggtttggat acggctattt gatattactc
      121 attcaattat caaatcacag taggtgtgaa tctcatcggg atcggcaata tctacttctg
      181 attaggatca aaggtgattt tctcaataaa ggtgtgatac aaagctttaa cttgcttctt
      241 ctcactctgg ataagaaagc aatccaaact agtcaatatc tgttggacat ctttgacttg
      301 aatcggttcc ccttgttgtt gaaagatccc taagatttca ttctctcgaa tttgtaactg
      361 tcttcgtttt tcagtcaatt catttaaacg atcttttaac atcggaaaca attcgggact
      421 atcttccaaa gcagcttcct gtttttgctc taatagttag attttgcgta ccattttctt
      481 cttttatttt ccttcgattg ctgattttaa tatagtctaa aggaaattta tatttattgt
      541 attctttata acttcaagag aacttcgttt gagtattact gtattttttt aattccataa
      601 gaaaattagt tattacctgc cacctaatca gcaaatctga acctgagtca atataattaa
      661 tattattttc catctccaac attgtaagta ctgttggtgt taatataggg actttgtaca
      721 attgtatttt tttgatttta tcatctgcac aaaggagatt ttttaaatct aatccttctt
      781 ttatttcaaa agttgaattt tttaaatcta taagagtaaa accaattcct tcatactgaa
      841 tttctgaaaa tcgaattttt ctcccagttg atagatcaag gggcagaaaa ccttttatta
      901 cattgtgagt gtcaccatca tttacaatgt cattaggaat tttgatttta gaattctgtg
      961 gagcacctat tggaagataa taattcatta taattttgta gtcttcatga ggaaataaag
     1021 tacttttagg attgctaaga tattctcgta ccgaatctaa gaatggctta tccattgaat
     1081 cttcatcccc atttaaacta aa
//
LOCUS       DACDHJ010000352         1099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.356, whole genome shotgun sequence.
ACCESSION   DACDHJ010000352 DACDHJ010000000
VERSION     DACDHJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.356"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            164..715
                     /locus_tag="IV223_002660"
     CDS             164..715
                     /locus_tag="IV223_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAP8712895.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          410 a          157 c          212 g          320 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccag
//
LOCUS       DACDHJ010000353         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.357, whole genome shotgun sequence.
ACCESSION   DACDHJ010000353 DACDHJ010000000
VERSION     DACDHJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.357"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..247
                     /locus_tag="IV223_002661"
     CDS             <1..247
                     /locus_tag="IV223_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIA"
                     /protein_id="HAP8712896.1"
                     /translation="TCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPEL
                     PGTMYVEDKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            231..926
                     /locus_tag="IV223_002662"
     CDS             231..926
                     /locus_tag="IV223_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8712897.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          403 a          174 c          216 g          289 t
ORIGIN      
        1 aacttgtgat atgaagctct gttttgataa acaaatgtat gtaatcacag cagtaggaga
       61 tgttgtccaa actaatttga atgaattagg acatatcacg ataaaatttg atggatctca
      121 tgagccggaa cttccaggaa caatgtatgt agaagataag gaaatgccgc atttaagaaa
      181 ggggacaatc attaaaatcg atcaagagga tcagaatggg ggaaagctat atggaaatca
      241 tgcttgatac agcaaataca aaaagtattt attcttgtca acagtttttg aatttaacag
      301 gcgtaacaac aaatcctaca attctgaaaa aagaaggagc gtcaaacttc ttctcgcata
      361 tgaaagaaat caaaaaaata atcggacaga ttcctatcca tattcagaca ataggaagaa
      421 cggaagaaga aatgatagag gatgctcgag ttatcataga agaactggga aaagatacgt
      481 ttatcaaggt gccggtgaat caagcaggcc ttcaagcgat caaaaagttg aaaaaagaag
      541 gttatttgat taccggtaca gctatttaca cagaattaca agggtattta gctattaata
      601 atggagcaga ttatattgca ccttattata acaggatgca aaatcaaggc attgatgcta
      661 tgaaagtgat tgattcgttg cggactgaga tcgaacgtac gcaaagacca acaaaaatat
      721 tagcagctag tttcaaaaat tgtgaacaag tcactacagc cattcgatct ggagctcatg
      781 cgataacgtt aggtaaagag ctactagatc aagtattaga gatgccctcg attgataaag
      841 cagtgagggg cttccaagaa gattgggtgc aaactttcca agcagaaacg attggtgaac
      901 taaaaaaggt cgctgattac ttctgaatca atttctccat aaaataaacg atagccttct
      961 cgaaaactcg aggaggctat ctgacgattc aatattcatt aatttaaagt ttgggtgact
     1021 attactaact attacttatt tctcatttaa acgatgtatt ccctaacctc taatacatcc
     1081 tt
//
LOCUS       DACDHJ010000354         1069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.358, whole genome shotgun sequence.
ACCESSION   DACDHJ010000354 DACDHJ010000000
VERSION     DACDHJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.358"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1025
                     /gene="uidA"
                     /locus_tag="IV223_002663"
     CDS             <1..1025
                     /gene="uidA"
                     /locus_tag="IV223_002663"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8712898.1"
                     /translation="ENKVIDTYTEEFGIRSIQVSNGKFLINNKPFYFKGFGKHEDTYI
                     NGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHL
                     NFMATLLDDNVEKHDTWKEIRTENAHKMAIKELIERDKNHPSVVMWSIANEPDSDSEG
                     AKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDICKVGDIVDVLCLNRYYGWYVSGGD
                     LKHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGLHDTVPTMFTEEYQVGYYEANH
                     EVIDKCDNFVGEQVWNFADFETSQGILRVQGNKKGIFTRDRKPKMIAHYLKKRWSSIP
                     NFSYKK"
BASE COUNT          391 a          137 c          227 g          314 t
ORIGIN      
        1 acgaaaataa agtgattgat acgtacacag aagaatttgg cattcgtagt attcaggttt
       61 cgaatgggaa gtttttgatt aataataaac ctttttactt taagggattt gggaagcatg
      121 aggacacgta tattaatggt cgtggtttga atgaagcagt aaatatcaaa gattttaatt
      181 taatgaaatg gatcggtgct aattcattta ggacttcaca ttatccttat tcagaagaaa
      241 ttatgcgctt agcagatcga gaaggtattg tagttattga tgaagtacct gcagtaggat
      301 tacatttgaa tttcatggct actttattag atgacaacgt agaaaaacat gatacttgga
      361 aagaaatacg gacagaaaat gctcacaaaa tggcgattaa ggaattgata gaacgcgaca
      421 agaaccatcc gagtgtcgta atgtggtcta ttgcaaatga accagattcg gattctgaag
      481 gcgctaaaga atattttgaa cctttagttg aattgactaa agaacttgac cctcagaaga
      541 gacctgtaac aatcgttact tacttaaagt caacaccaga tatttgtaaa gtaggagata
      601 ttgtcgatgt tttatgttta aatcgctatt atggttggta tgtttcaggt ggagacctaa
      661 aacatgcaaa atatctgttg aaaaaagagc tagatggatg gatggaaaga tgtcccgata
      721 cgccaataat gtttacagaa tatggagcag atacagtagc tggacttcat gatacagtac
      781 caacaatgtt tacagaagag tatcaagtgg gttattacga agcaaatcac gaagtaatag
      841 ataaatgtga caattttgtt ggtgaacaag tgtggaattt tgcagatttt gaaacaagtc
      901 aagggatttt aagagtgcaa ggaaataaga aaggaatctt tactagagat agaaagccta
      961 aaatgattgc tcactatcta aagaaacgat ggtcatcaat accaaatttc agttataaaa
     1021 aataatttac cttttataag gaatataaaa aaggagtggc tgcttaatt
//
LOCUS       DACDHJ010000355         1068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.359, whole genome shotgun sequence.
ACCESSION   DACDHJ010000355 DACDHJ010000000
VERSION     DACDHJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.359"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(255..569)
                     /locus_tag="IV223_002664"
     CDS             complement(255..569)
                     /locus_tag="IV223_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712899.1"
                     /translation="MAEENKVDPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIKENSQEEDGNIIISVEKFLELFSEIFEIDTDYYQPQEVFREFRNQIIRSGRT
                     VN"
     gene            complement(820..1068)
                     /locus_tag="IV223_002665"
                     /pseudo
     CDS             complement(820..1068)
                     /locus_tag="IV223_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          344 a          200 c          123 g          401 t
ORIGIN      
        1 acatatcaag aattttagac tattatttcg aaataaatac gatctgtgcg atcataacac
       61 aagatatacc tcataattat atacctcttt tttaccccaa tatttctctt gtagcacatg
      121 tggatgtaac aataggttat tatttattct aaagacgaca agtagtcact taaacaaacc
      181 ttagacatct gtaaaatctt caaaaaaaga agcgttgaaa gacggaactt ttcttcaacg
      241 cttctttttt tgatttagtt tacagtccta cctgagcgaa taatctgatt tctaaactct
      301 cgaaaaactt cttgtggttg ataatagtct gtatctattt caaatatttc cgaaaacaat
      361 tcaaggaact tttctactga aatgataatg ttaccatctt cctcttggct gttttctttt
      421 atttctgctt ctaattcaaa taaatctagt gacgaactaa aatcataaat cctgtcatat
      481 tgaacaaaaa aatcaagaaa aagttgctca aactcttcct tatttatcac tagtgtttca
      541 agcggatcaa ctttattttc ttcagccatt cttccaattt cctcactttc attttcaaaa
      601 atcacaaaaa tgatattaac ttaaaagcca ctaataaaat aagacttaca aagtgaaggg
      661 taagccaaca aaataaccat ttgctctacg tactacgatt tggtaatcct gcctttcact
      721 tggtaattca taatagtaat ttattgtttc tgctctgcca ctttcaatat actctggatt
      781 atctaatatc atagcatact cataaagctc ttcaccatcc ttattttcaa tcgaccattg
      841 ttgcggacat atcggatttt ctgtatcatt tttaagtgta taggtcactt gtaatacacg
      901 atcatgttgt gcaaattcat ttcttttttc ggtcaattca atatttttaa atgttaattc
      961 aaccccatct gcctgataag tttcacctat ttttaaatct tttgttacaa tttttattgg
     1021 cttttcttct ggaaaatatt ttgctttaat tgttaaataa cttccaaa
//
LOCUS       DACDHJ010000356         1065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.360, whole genome shotgun sequence.
ACCESSION   DACDHJ010000356 DACDHJ010000000
VERSION     DACDHJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.360"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1065
                     /locus_tag="IV223_002666"
     CDS             <1..>1065
                     /locus_tag="IV223_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8712900.1"
                     /translation="KPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVD
                     SKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYF
                     GVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPI
                     GKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAF
                     GLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFD
                     FEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVG
                     ICPVSYFEAPIHVCIDFSQG"
BASE COUNT          367 a          160 c          200 g          338 t
ORIGIN      
        1 caaagccaga gatccaaatt tcagaagaag cagagatcca tgtcgaacgt ctgaatttga
       61 cggatatcaa aaatttaaga agaatctact ttcaacgatc aaaaattgct cgactgttaa
      121 cggagatcgt ggattcaaaa gtaacgatcg aaaaatttgc agagaatcat tttttaagca
      181 tatcaagagc ctatgtaaaa agaagagaat tagtctcttt tcttgaacag catcaaataa
      241 agttaaagaa aaacatatta gttggggaag aaataaacat acggaacgta ttatttgggg
      301 tatatttcgg ggtctttaat ggtttttcta tgccatttag agaaagcgtc atgaatgaag
      361 caaagagtat cgtgcaatat tttgaatatc tgttcaattt aaaactgtct gcaacaaaac
      421 gaatcaaatt caatttgcta accactatta gtttgttgag ggcctatggg aatcaaccaa
      481 tcggaaaacg tttcttttca gatgaatact ttaagagctc agaaggaatg aagatcatca
      541 aggatttatc acatatttct tttcttcata tccctaaaga gcagatcatt gatgagattt
      601 cttatatcat gttgtttttg atcacggaag aagaatttga attagatggc gcatttggac
      661 ttgcggaagc tcattttcat gatatccaca tgattagtca aaaaatgtca gtggaattga
      721 ttgatcaatt accattttcg attgaaatct ctgaagaaga aagactatat atacgaaaag
      781 aacttcgtat gggtatgatg catgtcaatc gaaaaaattg gatattcgac tttgagatga
      841 ctagctttac gtctaaaaag cagctacagt ttttcttcga gacgtatcca atattcagta
      901 cagcaatcca aaaggtcata caagagaata aacgctattt cttgggaaaa gatgaacgtt
      961 tcatgattcg cttattttat gattatctct ttttattggt cggtatttgt ccggtatcat
     1021 attttgaagc accgattcat gtctgtattg atttttcgca aggat
//
LOCUS       DACDHJ010000357         1037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.361, whole genome shotgun sequence.
ACCESSION   DACDHJ010000357 DACDHJ010000000
VERSION     DACDHJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.361"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..363
                     /locus_tag="IV223_002667"
     CDS             <1..363
                     /locus_tag="IV223_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8712901.1"
                     /translation="GYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARK
                     IGQALAGGVGGWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYP
                     LSKEKVLENYNLLNQKHN"
     gene            374..>1037
                     /locus_tag="IV223_002668"
     CDS             374..>1037
                     /locus_tag="IV223_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8712902.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPI"
BASE COUNT          372 a          120 c          210 g          335 t
ORIGIN      
        1 ggatatatgg ggttaggtat ttttaataca gtcatttggg cgaatattac tgatgtaatc
       61 gatgatcaag aagtgaaatc tggccaaaga gaagatggaa ctatttatgc agtatattct
      121 tttgcccgaa aaattggaca agctttagct ggcggtgtcg gaggttggac tctttctata
      181 atagggtatg attcgttagc aaaagtacaa agtggagaag ttttacaaaa gctgtataac
      241 tcttcaacgt tgattccagc agtttgtttc tttgttgtag gattgatttt atttttcgta
      301 tatccattat ctaaagaaaa agtattagaa aattataatt tgctaaatca aaaacataac
      361 taggagaaaa aacatgaaca gttggatatt ttgtattgat tcagatggtt gtgtcatgga
      421 tacaatgact tataaacaca atctcttttt cgggccaatt gctgcagaga tttttgatgt
      481 tcatcatagg gaaaaattcc tacaggattg gaataatatt aatttatatt cagagactcg
      541 tggaataaac agatttttag gtttagttaa aacattagaa aaaaatgatg taccatcgat
      601 taatcgatta aagcactggg tatctgtaac aaaagaattg tctaatccat ctttgaagag
      661 ggaaatagaa tactctcagt cagaggattt attaaaagct ttaaaatggt cggaagaagt
      721 caatcgacag attagtcaag ctaaaggatt agataaaccc tttttaagtg ctagagatac
      781 tataaaagct ctgaaaaaat atgggaagat aattattgta agctcagcta ataaggaagc
      841 tattcaagaa gaatgggaaa gacatgaatt attatcctta gttgatgaac tgtgttgtca
      901 agataaagga aaaaaagaag acattattcg aagtgtaatt gaaaatggtt gtgatttgga
      961 taagatatta atgattggtg attctcctgg tgatctagaa gctgcaaata agaataaagt
     1021 tttcttttat cctattt
//
LOCUS       DACDHJ010000358         1037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.362, whole genome shotgun sequence.
ACCESSION   DACDHJ010000358 DACDHJ010000000
VERSION     DACDHJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.362"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1037)
                     /locus_tag="IV223_002669"
     rRNA            complement(<1..>1037)
                     /locus_tag="IV223_002669"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          235 g          260 t
ORIGIN      
        1 gtagcacgtg tgtagcccag gtcataaggg gcatgatgat ttgacgtcat ccccaccttc
       61 ctccggtttg tcaccggcag tcttgctaga gtgcccaact gaatgatggc aactaacaat
      121 aagggttgcg ctcgttgcgg gacttaaccc aacatctcac gacacgagct gacgacaacc
      181 atgcaccacc tgtcactttg cccccgaagg ggaagctcta tctctagagt ggtcaaagga
      241 tgtcaagacc tggtaaggtt cttcgcgttg cttcgaatta aaccacatgc tccaccgctt
      301 gtgcgggccc ccgtcaattc ctttgagttt caaccttgcg gtcgtactcc ccaggcggag
      361 tgcttaatgc gttagctgca gcactgaagg gcggaaaccc tccaacactt agcactcatc
      421 gtttacggcg tggactacca gggtatctaa tcctgtttgc tccccacgct ttcgagcctc
      481 agcgtcagtt acagaccaga gagccgcctt cgccactggt gttcctccat atatctacgc
      541 atttcaccgc tacacatgga attccactct cctcttctgc actcaagtct cccagtttcc
      601 aatgaccctc cccggttgag ccgggggctt tcacatcaga cttaagaaac cgcctgcgct
      661 cgctttacgc ccaataaatc cggacaacgc ttgccaccta cgtattaccg cggctgctgg
      721 cacgtagtta gccgtggctt tctggttaga taccgtcaag ggatgaacag ttactctcat
      781 ccttgttctt ctctaacaac agagttttac gatccgaaaa ccttcttcac tcacgcggcg
      841 ttgctcggtc agactttcgt ccattgccga agattcccta ctgctgcctc ccgtaggagt
      901 ttgggccgtg tctcagtccc aatgtggccg atcaccctct caggtcggct atgcatcgtg
      961 gccttggtga gccgttacct caccaactag ctaatgcacc gcgggtccat ccatcagcga
     1021 cacccgaaag cgccttt
//
LOCUS       DACDHJ010000359         1018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.363, whole genome shotgun sequence.
ACCESSION   DACDHJ010000359 DACDHJ010000000
VERSION     DACDHJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.363"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..264
                     /locus_tag="IV223_002670"
     CDS             25..264
                     /locus_tag="IV223_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712903.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(626..886)
                     /locus_tag="IV223_002671"
     CDS             complement(626..886)
                     /locus_tag="IV223_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712904.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          335 a          186 c          139 g          358 t
ORIGIN      
        1 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
       61 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      121 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      181 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      241 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
      301 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
      361 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
      421 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
      481 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
      541 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
      601 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
      661 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
      721 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
      781 caacacaatt ttctaattta ctgattactt ctccaaccac tttttgtttc aagccaatag
      841 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
      901 taatgttttt ccattattta aaatgataat accattaaaa tatgattatg aaaatcaata
      961 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctt
//
LOCUS       DACDHJ010000360         1011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.364, whole genome shotgun sequence.
ACCESSION   DACDHJ010000360 DACDHJ010000000
VERSION     DACDHJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.364"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1011
                     /locus_tag="IV223_002672"
     CDS             <1..>1011
                     /locus_tag="IV223_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8712905.1"
                     /translation="IIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCAL
                     MLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLMA
                     VLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYALR
                     FNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFIP
                     FTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPPV
                     AASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTTL
                     IC"
BASE COUNT          286 a          148 c          199 g          378 t
ORIGIN      
        1 ctattatagc aattttcaca acgttgaatt tatctgcagg tgtctataat aaaggactaa
       61 ttaaatacga gaataaatat gcattaacct cgtcattttt aggattatcg tcatttattt
      121 ccctttgtgc attgatgcta taccttccat ttagaacggt tataaatggg ctactggatt
      181 tatcgacgga gtttgtcata ttgattctta ttgaatcaat atttgagccg gcttttttgc
      241 tttggtcagt taaggaacga tttgattaca agtatcaaaa actagtactc atcacagttc
      301 ttatggctgt tttaaatcca ggtctcggta ttatattaat cttatcattt ggggagtatg
      361 ctatttggag agtacgagct tatgtcgtgg taaatgttgt agtggggatg tttattggca
      421 taggaatact ggctaagggt aagaaattat ttgataaaga gtactggaca tatgcgctca
      481 gatttaattt gccattaata ccacattatt tatcaatgac attgctgaat caggttgatc
      541 gccttatgat aaatagtatg gttggagcta gcgaggctgc gatttatagt atcgcttaca
      601 ctatatctag tatgatgaat atcataacaa cagctataaa cggttctttt attcctttta
      661 catacaaagc aattaaagat ggcgtgtatg aaaaaattaa atccgttgct aatcaactat
      721 tagtgttgat ttttgtcttg tgttcgttta ctatgctgtt cgggccagaa ttgatttggg
      781 ttgtcggagg agaaaaatat atggatgctg tttggattat tcctccagtt gcggcatcag
      841 tatttttcat gtttttatat ccgctttttg gcaatattga attttactat gaaaagacaa
      901 actacgtaat gtttgcatct tgtgtgggct ctatcgcaaa aattatttca aactatgtta
      961 gtattccccg ttttggatat atttcggctg gttacacgac attgatttgc t
//
LOCUS       DACDHJ010000361          999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.365, whole genome shotgun sequence.
ACCESSION   DACDHJ010000361 DACDHJ010000000
VERSION     DACDHJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.365"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            346..981
                     /locus_tag="IV223_002673"
                     /pseudo
     CDS             346..981
                     /locus_tag="IV223_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          340 a          182 c          179 g          298 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttacctt atattatttg tcaaattaa
//
LOCUS       DACDHJ010000362          977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.366, whole genome shotgun sequence.
ACCESSION   DACDHJ010000362 DACDHJ010000000
VERSION     DACDHJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.366"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(215..889)
                     /locus_tag="IV223_002674"
                     /pseudo
     CDS             complement(215..889)
                     /locus_tag="IV223_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          288 a          184 c          136 g          369 t
ORIGIN      
        1 taatagtctt aattcattaa aataaaaaac tcaactttac aaggatatgg tttaaaaaat
       61 ttatttatcc aacccaacta gttttcgctg cttacgattt gcttcatctg ctacgtaatt
      121 cattactgtt ttaaattctt tcgctgaggc gttttctgat aaatcagcga tacttttatt
      181 atacttcttc acgatttgtt gtacttgttc tttctcagtc atgtaattcc tcctccaact
      241 cttttgaagt atattgtatt ggtagtacta attgaatatt atctgctgca taatgcactt
      301 cagaaatgtc gttttgataa tccttaatca ttatgtgaag gactacttca ggatgtctat
      361 ttttgacttt ttcaatattc tctctggact taaaaaacgc attgataaac atttcttttg
      421 gcacatgacg tccttcagct agttcacgtt ttcgagtgaa atcccaggca actttgggat
      481 cttggtaaac atagaaaatc gtaacgcgaa agttattttt aagggctcga ataatatttt
      541 tctctgcact aaggatagca aaagtatcct ctagtatgaa agaatagcca tgttccagta
      601 catattttaa tgattgttca actaaccaag aagaagcttt ctgaaaaaca cttgagttct
      661 ttccattgta ttctggaaat tgtgctctaa aatagtccgc atcaatcaca acatggttgt
      721 cataatttta agctaaacct agtgcaacct ctgttttacc tgctcctggg cttccagcca
      781 tgaataccgc atctttttca tcatcaggga tttttccctt gatgattgtt tcaataaatt
      841 tatctttatt gaccctagcg taatctaaat acttgtcttt aggttccaag attgcacctt
      901 ctttccttct tctatattca tactaattat acgatattta tataaaaaat ataacggtca
      961 ttttaaccat attaaca
//
LOCUS       DACDHJ010000363          964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.367, whole genome shotgun sequence.
ACCESSION   DACDHJ010000363 DACDHJ010000000
VERSION     DACDHJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.367"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..97
                     /locus_tag="IV223_002675"
     CDS             <1..97
                     /locus_tag="IV223_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8712906.1"
                     /translation="VSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            195..>964
                     /locus_tag="IV223_002676"
     CDS             195..>964
                     /locus_tag="IV223_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712907.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNE"
BASE COUNT          375 a          106 c          189 g          294 t
ORIGIN      
        1 tgtttctcaa tacgggttac ctgttataaa aattgatacg gtcattagat ttaaaaaatc
       61 tgatattgat agtttcatgg aacggtataa aggttgagta aacaaataag actaaaacat
      121 atagtaataa agagtttgtt tttattatct gtatgtttta gtctttaaca gaattttata
      181 aggaggaaac acaaatggaa acatcaatga ttaatattcc aatttatgta gatcaagaaa
      241 aattattaga gaagccagtt acaaaaaaag aaaaaactga tgtaagagat aggtgtttag
      301 ataactatag agaagttaca agagatgagt ttgtagagat actaaattca tcacaatcat
      361 ttattccaag caagacaaag aataaaggca atgacaagga gagttttgtg gaaaccagag
      421 taattattct ggatgtcgat aacactgtta aagatgagaa ggggaaggta gtagatttga
      481 gtaaagatga tagtagatac ttaagcatag aaaaggtttt atctatcaat ttagttcata
      541 attctgcgtt tgcgatacaa aaaagtattc ggtactctga aaatctagaa aaatacaaga
      601 ttgtctttct attaaaagaa gcaattactg actatggtaa aatggttgct gtttacgaat
      661 acttgaaaaa gaacatcccc gggtgtgatg acaatgtaaa tgcttcgaat agaatgtttt
      721 ttggaggata taagtcagat gcagtgataa taattaatga agacaacatg ttagatatta
      781 ctgagcttcc gattgatttt accatgacat gttctaaaaa ttgtcaaagt gattcaggga
      841 gagagtatga actagtcaat gaaaccgatt ttgttaagtt gataaaaaat gacgacaaag
      901 aagaaatgaa acagtggttt aaagactgcc tgtttgattc ctccgacatg tcttttaatg
      961 agat
//
LOCUS       DACDHJ010000364          963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.368, whole genome shotgun sequence.
ACCESSION   DACDHJ010000364 DACDHJ010000000
VERSION     DACDHJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.368"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..892
                     /locus_tag="IV223_002677"
     CDS             <1..892
                     /locus_tag="IV223_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8712908.1"
                     /translation="SVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAG
                     LLKQKRGRVNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAK
                     PRKINDSTVIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQ
                     VLLDNFVDIMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTI
                     VVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLA
                     NEQRETFNELPTVSTENWLE"
BASE COUNT          397 a          143 c          160 g          263 t
ORIGIN      
        1 ttcagtcaaa aataactggg tagattctga tgataatata ttcttcatat tcacagttga
       61 agaattaatg aaattgttac aatgccgcga aggtaaagta tctaagatca aaaaagaatt
      121 agaaaaagca ggtcttctga aacagaagag aggaagagta aataaacgtg atggtaagat
      181 tgaatctatg cctaatttat tgtacttagg caagccagaa gttacgaatc aagacgtctt
      241 taaaatcatg gaagaggaag acaatactga ttccaccgta attgcgaaaa tcgcaaatac
      301 ggcaaaaccg cgaaaaatca atgattctac cgtaattgcg aaaatcgcaa atacggcaaa
      361 atccagtcat atcaaggact caaccgtaat tgcgaaaatc gcagacaatc tattctattc
      421 taattctata gacactaata gacacataat agacactgaa actgaccagt tacaaaatca
      481 agttttgcta gataacttcg tcgacatcat gcaagaggac agtataaata catttgtacc
      541 agaaaatgta ttaaacttga taaaaacatt ttcttcaagt tacagtgaag ctcaacaaac
      601 cgtccaaact attcataatg caaagaaaaa agctgaagaa ttagagcaaa ctattgtcgt
      661 ttatgaagaa ctggaatctt atggaattga tgctgacaaa ggattatata tgactttact
      721 gaaagcttat caaaaacaaa aaactgaaaa agtaaacaat ttgcagaatc taattttcgt
      781 ttatgtaaaa aattggtttg tcgaaaaagc tattgcggca aagcttgcaa acgaacaaag
      841 agaaacattt aacgaattac caacagttag tacggaaaat tggttagagt agttgataaa
      901 aagcaattac tataaaaatg atcaatttta aattagaact tttttatgat tttaatttta
      961 tta
//
LOCUS       DACDHJ010000365          955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.369, whole genome shotgun sequence.
ACCESSION   DACDHJ010000365 DACDHJ010000000
VERSION     DACDHJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.369"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(67..297)
                     /locus_tag="IV223_002678"
     CDS             complement(67..297)
                     /locus_tag="IV223_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712909.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(391..729)
                     /locus_tag="IV223_002679"
     CDS             complement(391..729)
                     /locus_tag="IV223_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712910.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          304 a          194 c          107 g          350 t
ORIGIN      
        1 aacagcaaag atatcgaaaa aacaataaca taatatgtgt caaaattttt cataataaga
       61 actcccttac tgttgcatac taacataatc caaaaactct tctacttcag ctaaagacat
      121 tccactttct ttagtccaaa aaacagtttt ttctatcgat ccttctctgt aaactgtaaa
      181 tgtcggagtc gagttaattg agtagcgact cttgaattct ttccactcat tttcatcttc
      241 tctaactttt gctacattca cgtaataaat attttctttt cccttttcta ccagcatatc
      301 gaatagcttc ctgcttttga tgatcgattt cttgtgctac atctagtaaa ttttcatcaa
      361 tttctatatc ataaagtatt tcactagtgt ctacatccac aatatatact ttttatcagt
      421 agcaattttt gagtaatccg ttaaaatgat ctcagtctcg tcactttcat cagtataaat
      481 atacgttaac aatgaactca ccttttcaat cggtgttagc tctaagccta ctgtattttt
      541 tactattgag tcattattta ccaccataac ttcgtactgt atataccatc ctatttggtt
      601 tccatcaata tcagtaattg gctcactagc taatggagtc tccggaacaa tattttcctg
      661 atatttctta aactcttctg tagcacgaaa aaggttatca tgttcctcac ttgaaactgg
      721 ttgagtcata gaaaatgtta cttggctaaa cagagggaaa ataaacacta caatcaacaa
      781 agtataaaaa aaagcccttc tttttcattt tttatcttcc aatcacaaat tgaaaaactt
      841 attcctgcaa ctttgagtct agtatcacaa tcgactgact acaactattc atttacattc
      901 tactttaagt tcctccttaa ccattaattt tattaaagat acttgtaata gtgat
//
LOCUS       DACDHJ010000366          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.370, whole genome shotgun sequence.
ACCESSION   DACDHJ010000366 DACDHJ010000000
VERSION     DACDHJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.370"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IV223_002680"
     rRNA            2..>943
                     /locus_tag="IV223_002680"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDHJ010000367          942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.371, whole genome shotgun sequence.
ACCESSION   DACDHJ010000367 DACDHJ010000000
VERSION     DACDHJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.371"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..756
                     /locus_tag="IV223_002681"
     CDS             <1..756
                     /locus_tag="IV223_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8712911.1"
                     /translation="TVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYN
                     QQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENP
                     ENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVF
                     TMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGH
                     ASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
BASE COUNT          351 a          132 c          169 g          290 t
ORIGIN      
        1 acggttattc attatcacgc atttatacgc aaagctcttc aagaggctgt tattacagaa
       61 ttgataagta caaatgtagc agataaagta agaaagccaa aaaagaaaca atttgtttct
      121 caagtttata atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc
      181 tatttggtag ttcttttaac agcattttat ggattgcgaa gaagtgaagt tttaggatta
      241 aaatggtctg ctattgattt tttaaacaaa aaaatcatga taaaccatgt aatcatagaa
      301 aatccagaga atatgtcaca gttaataaag aaagatcaaa caaagaactc ttccagctat
      361 cgtacattac ctttggttga aacgattgaa cgagcattag taaatcaagc taaatggcaa
      421 caagataatc gtattctttt aggagatgat tatattttag aagataatga gtacgtattt
      481 actatggaag atggacggtt gatgaagcct caatatgtaa cgcatcgttt gagtaagttg
      541 attaagaaaa atggattgaa aaaaattcgc tttcatgact tgcgtcattc taatgcttca
      601 ataatgttag atagtggcca gaatatgaaa tccattcaag aatggctggg acatgcatcg
      661 tattcgacaa ctgctaattt atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca
      721 gaagcattag aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg
      781 ggtatcgctt cttgttttta gaattttttt gttggcaatt tgttggcagt atgttggcaa
      841 tgaaaaaatt cacaaactaa aaacctattt ataaagcttt ataaaacttt tttcacaaat
      901 agagcatttg gttaagagtc agctgctcta ccaactgagc ta
//
LOCUS       DACDHJ010000368          935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.372, whole genome shotgun sequence.
ACCESSION   DACDHJ010000368 DACDHJ010000000
VERSION     DACDHJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.372"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>935
                     /locus_tag="IV223_002682"
     CDS             <1..>935
                     /locus_tag="IV223_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8712912.1"
                     /translation="HTTYKELSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKG
                     VSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRST
                     VESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTD
                     LLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERI
                     ETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHD
                     LDQTKLSYLDMGVELQELVRIKLNEFYPDEELINN"
BASE COUNT          367 a          135 c          159 g          274 t
ORIGIN      
        1 gcacacaact tacaaagaat tatcagaaca attatatgta tctacgaaaa caattagcaa
       61 agatatttta attttgaaag aaataatcca aaaaatgcca gatgtacaaa tgttggttca
      121 acctaaaaaa ggcgtttcgc ttattggaaa aaaagaaaca cttcgaaatt tattatccag
      181 tcataaaatc aatatcgcag actctccgga ggaccgtcta acttatgtgt ttgctaaatt
      241 attgaatact aatgattctc taaagattca aaatcttgct gacgaattat acgtgagcag
      301 gtcgacggta gaaagcacaa tgaaagatgt tagaaaaaga ctaaacaagc atgggatgtc
      361 cttagtttca gatcgaaatg gcgtgcgcat caaagctgat gaaaaaagta aaagaaggtt
      421 gatgtcagaa gttgtgaact attattgggg tggctttaca gcatctaaca gtaaaacaga
      481 tctcttagac ttgaatatca atttcattgg tgaagcgaat aatttcttaa ataaggacat
      541 attgaaaaaa gtagccgaat tgcttaatcg atacatgaat aagacacaat tgaaatttac
      601 agattatgaa tttcaaactt tagctatcca ccttgctatt gcgatagaaa ggattgaaac
      661 aagcaatttc ttatcacgta gtaataattc tgaagaagaa atctcaaaga atacacttat
      721 tttaatcaaa atgattgaaa aagcattttc aattattgtg ccaatagatg aacaagctta
      781 tgttgacatt cacattactt cgattgaaaa aaatgctatg aatcacgact tagatcaaac
      841 gaagctttcc tatttagata tgggtgttga gctacaagag ttagtaagaa ttaaattaaa
      901 tgaattttat cctgatgaag aattgatcaa taatc
//
LOCUS       DACDHJ010000369          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.373, whole genome shotgun sequence.
ACCESSION   DACDHJ010000369 DACDHJ010000000
VERSION     DACDHJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.373"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            92..>925
                     /locus_tag="IV223_002683"
     CDS             92..>925
                     /locus_tag="IV223_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8712913.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QR"
BASE COUNT          277 a          184 c          175 g          289 t
ORIGIN      
        1 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
       61 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      121 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      181 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      241 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      301 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      361 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      421 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      481 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      541 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      601 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      661 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
      721 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
      781 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
      841 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
      901 taaaggaaat tcccactctc aacga
//
LOCUS       DACDHJ010000370          923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.374, whole genome shotgun sequence.
ACCESSION   DACDHJ010000370 DACDHJ010000000
VERSION     DACDHJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.374"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>923)
                     /locus_tag="IV223_002684"
     CDS             complement(<1..>923)
                     /locus_tag="IV223_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8712914.1"
                     /translation="HLDEDAISWIEETIDAMTLEEKIGQLFVNMGSSRDKNYLSSMVN
                     QYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKI
                     GATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQSPDKVLE
                     LSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWDNSFGKVYSGLIE
                     AGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDAS
                     HMLGMTGAMKRSELLPTSIAAGCDLFLFFND"
BASE COUNT          299 a          193 c          128 g          303 t
ORIGIN      
        1 gatcattaaa aaataggaat agatcacatc cagccgcaat agaagtgggc aataattctg
       61 accgtttcat tgccccagtc attcctaaca tatgactagc atcagtaact actaatccat
      121 taaacccgag ttttcctcta agtaagtcag tgattaattc ttttgataaa gttgcaggaa
      181 gtaaatcttc atctttcaga tcaagattaa aatgttttga ataagctggc atatggatat
      241 gacctgccat aattgaaggt aatccagctt ctattaagcc tgagtatact tttccaaaag
      301 aattatccca ttcttctgta cttaaactat taatactaaa agatagatgt tgatctcgtt
      361 cgtcaatacc gtcaccaggg aaatgtttag ctgcaggcaa aattccactc tcttgaatac
      421 ctttcatata agctaaacta agttctaaaa ctttatcagg gctttgtcca aaagatcgac
      481 tagaaatgat aggatttctc caatttctat taatatctac aataggagca aaactccagt
      541 tacatccaat agccgatgct tcaattccag aaactctccc taattcataa gcatatttcg
      601 catcatttgt tgcaccaatt tttacttcta caccaacttc ggtaccatct gtacaagctc
      661 cgttaccacc tgcttctgta ttagcagcaa ttaataaagg aattttgctt tctgtttgta
      721 ggatatagtt ttgatcatat acctcatcag cagttcctgg attatatcgt acagctccaa
      781 tatggtactg attgaccata ctacttaagt aatttttatc acgactagat cccatattaa
      841 caaataattg acctattttt tcttctaaag tcatagcatc aatcgtctct tcaatccaag
      901 aaatagcatc ttcatctaaa tga
//
LOCUS       DACDHJ010000371          894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.375, whole genome shotgun sequence.
ACCESSION   DACDHJ010000371 DACDHJ010000000
VERSION     DACDHJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.375"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..808
                     /locus_tag="IV223_002685"
     CDS             68..808
                     /locus_tag="IV223_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8712915.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          314 a          157 c          229 g          194 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggt
//
LOCUS       DACDHJ010000372          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.376, whole genome shotgun sequence.
ACCESSION   DACDHJ010000372 DACDHJ010000000
VERSION     DACDHJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.376"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV223_002686"
     CDS             <1..888
                     /locus_tag="IV223_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8712916.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDHJ010000373          876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.377, whole genome shotgun sequence.
ACCESSION   DACDHJ010000373 DACDHJ010000000
VERSION     DACDHJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.377"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..434)
                     /locus_tag="IV223_002687"
     CDS             complement(<1..434)
                     /locus_tag="IV223_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8712917.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /locus_tag="IV223_002688"
     CDS             complement(451..855)
                     /locus_tag="IV223_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8712918.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DACDHJ010000374          868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.378, whole genome shotgun sequence.
ACCESSION   DACDHJ010000374 DACDHJ010000000
VERSION     DACDHJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.378"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..681
                     /locus_tag="IV223_002689"
     CDS             <1..681
                     /locus_tag="IV223_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8712919.1"
                     /translation="TKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQK
                     VFESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPI
                     ATSHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQAN
                     SSLAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVR
                     HTISEIKE"
BASE COUNT          292 a          154 c          155 g          267 t
ORIGIN      
        1 acaaaaaaac ttggttataa tggatttaaa gattttaaaa tggccatgtt gatccaagaa
       61 aatgatctat ctgctatttc tatccacgaa aatattcaga aaacagatac tgagctcact
      121 atggcgcaaa aggtgttcga gtctagtatt tcaactttaa atgacacaaa aaagttgtta
      181 caacaaaagg atctaaaaaa agcggctact attatcaatg aatctaaacg tgtctatttt
      241 ttcggtgttg gcggttcaga gattgtagca actgacgctt atcataaatt tttgcgttcc
      301 cctatcgcaa cttctcacag cacggattac catattcaat tgatggaagc ttctttattg
      361 acggaaaaag actgtgctgt tttgatttct cacacagggc aatcaaaaga aacaatccat
      421 attgctgaaa ccgtaagaaa aactggagca aaaacaatcg ttattactag ccaagctaat
      481 tcttcccttg ctaaacttgg cgatgtcgtt tttatttcta tttctgaaga aacagaattc
      541 cgatctgaag ctttagcttc ccgtatttct caactaagca ttttggactc tctatatgtg
      601 attttgatgt tttacaacga aaaagaatca cgtcgttcta tcgctaaagt tcgacatact
      661 atttctgaaa tcaaagaata gcaccataac agaaaaagcg aaaaatccat aaaacaaaaa
      721 aagccactac caaatatttg gtagtggctt ttttggttct gcaagggtgg tacagaacat
      781 caatagtcgg tgggttagaa cgtatgtcat cgcatctttg gagaaggttg tattggtatt
      841 attcgagtta cttgttagat gcgataac
//
LOCUS       DACDHJ010000375          866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.379, whole genome shotgun sequence.
ACCESSION   DACDHJ010000375 DACDHJ010000000
VERSION     DACDHJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.379"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(521..814)
                     /locus_tag="IV223_002690"
     CDS             complement(521..814)
                     /locus_tag="IV223_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8712920.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          257 a          157 c          129 g          323 t
ORIGIN      
        1 agagaaatgt gtcgactgca gtgttgtgtc cctcatcacg aataaacggt gttcaattag
       61 ctgatcttgt attcacttaa aaaatggtaa agtacgagtt gctattcccc attttcaatt
      121 ttctaacgct caggacaaaa tactgttttc acaacctcat tggaataaaa gcatatatat
      181 tgtttcaaga acgaaatctt gttatctttt taaagggcat ctgtccaaaa tctaatttta
      241 ggaggtacgt tttggtattt attcgtacaa ataaaagaga attatcttta ttcatgtaac
      301 gcatccaatt gattttttca tgtacgctcc tggaacgatt ttactgacga ttgcttattt
      361 aactgcgaat cgctcattag cttttatcat agctatccat attttgaata acgttcttgg
      421 ctttgtatta tagatacaga tgacaaaaaa agttcaagta tgcggtgtag aaccaatatc
      481 gcatacttga acttttttca atgagtataa attaaaactt ctaattttca attcccatca
      541 tctgatatgc ccgaccgtct gaacttcttt tgaaaaagcc aaatgtgatt agataacgtc
      601 tttgttcgac ataatcgcca ctagaaaata cgttcagcag tatcgaatta acctctgttt
      661 ctgtataagt cactccatgg tcaaagcgtt tcaagacttc tttcaatagg atgtatttct
      721 tttttgttga acttggtact tgttttactt ttccatcatt catgaatgtg tctaaaactt
      781 tcatcttttc ttgctccgta taagtataaa ccatcttctt tctccttttg attctaagaa
      841 tactccattt atgttagaat attctg
//
LOCUS       DACDHJ010000376          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.380, whole genome shotgun sequence.
ACCESSION   DACDHJ010000376 DACDHJ010000000
VERSION     DACDHJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.380"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..457
                     /locus_tag="IV223_002691"
     CDS             50..457
                     /locus_tag="IV223_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712921.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            471..>863
                     /locus_tag="IV223_002692"
     CDS             471..>863
                     /locus_tag="IV223_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712922.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATL"
BASE COUNT          324 a          111 c          153 g          275 t
ORIGIN      
        1 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
       61 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      121 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      181 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      241 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      301 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      361 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      421 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      481 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      541 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      601 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      661 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      721 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
      781 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
      841 ggaaatctgt agtagcaact ttg
//
LOCUS       DACDHJ010000377          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.381, whole genome shotgun sequence.
ACCESSION   DACDHJ010000377 DACDHJ010000000
VERSION     DACDHJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.381"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>863
                     /locus_tag="IV223_002693"
     rRNA            <1..>863
                     /locus_tag="IV223_002693"
                     /product="23S ribosomal RNA"
BASE COUNT          214 a          188 c          272 g          189 t
ORIGIN      
        1 atgcaggtta cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt
       61 gagtgtctgt accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc
      121 gatggaggcg ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa
      181 tcgtggtggg agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt
      241 aacggaggcg cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc
      301 agaagggagc ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga
      361 tccggtggtt ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac
      421 aggcttatct cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc
      481 gtcgcatcct ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca
      541 cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga
      601 aatttgagag gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc
      661 agttgttctg ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc
      721 atctaagtgt gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc
      781 ctgagagatg atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact
      841 gatactaatc gatcgaggac tta
//
LOCUS       DACDHJ010000378          861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.382, whole genome shotgun sequence.
ACCESSION   DACDHJ010000378 DACDHJ010000000
VERSION     DACDHJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.382"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..382
                     /locus_tag="IV223_002694"
     CDS             80..382
                     /locus_tag="IV223_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8712923.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGESFERR"
     gene            652..>861
                     /locus_tag="IV223_002695"
     CDS             652..>861
                     /locus_tag="IV223_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712924.1"
                     /translation="MNAIVILAEITYWYRPQKIFNDEGQLIGYKKKFIEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIK"
BASE COUNT          356 a           93 c          183 g          229 t
ORIGIN      
        1 acttcgcgat attttgtaac aaaaagtaat aatttgttag gtagagaagt tatagaacaa
       61 tcaaaaagga gaggtttttg tggaagcaat aaaattaatg atctcagaag agcaggagga
      121 aatgtttcta aagcagtttt atcagattgc tacaaaagcg attgagaagg ctcaagaaaa
      181 tgcgggtatg acaagagaat atctaaatca aaaggaaatg gcagaatact taaatgtgag
      241 cgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg cccgtcgttg aattaggggc
      301 aagaaaattt tattcgaaaa aggcagttaa tgagtttatg tttgctcgaa tgaagagagg
      361 tgaaagtttt gagagaagat gaaatagcaa atttagtatt gaaaagaatt gaaaaaaata
      421 tataaaagag tgctcccgat agtttggcga ccacaggaag cactcgacaa taaagttctg
      481 agaaaacttt atttatagct attatatcaa aaagaaggtg aaaaggtgtt ttcttctgga
      541 aatgttgtag ttgacaaact tagagaaata aattttgacg gaaatgtaat tccacattcg
      601 tggtacggtc aattaagaaa aaaaacgaaa aaaggcgttg agaagcccta tttgaatgca
      661 atagttatct tagcagaaat tacttactgg tacagacccc aaaaaatttt taacgatgag
      721 ggccagttaa ttggatataa aaagaaattt attgaagata ttttgcaaaa atcatataag
      781 caattaagca aaaaaacagg actatcagaa agagctataa aagatgcaat tgtgtactta
      841 gagaaaaagg gaattatcaa a
//
LOCUS       DACDHJ010000379          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.383, whole genome shotgun sequence.
ACCESSION   DACDHJ010000379 DACDHJ010000000
VERSION     DACDHJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.383"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>855
                     /locus_tag="IV223_002696"
     CDS             <1..>855
                     /locus_tag="IV223_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8712925.1"
                     /translation="EDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEI
                     TFTDQPAYVNSKGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPK
                     NIVSKLSFELTKFGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTAT
                     DLTLGKYYFVEVPSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKA
                     SYVNYKAPVIDKTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSET
                     SPYSVFKWG"
BASE COUNT          306 a          141 c          192 g          216 t
ORIGIN      
        1 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
       61 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
      121 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
      181 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
      241 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
      301 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
      361 agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
      421 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
      481 tgatttgact ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
      541 agatcaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca
      601 taataaatta acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta
      661 cgtgaactat aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca
      721 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
      781 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
      841 cgtatttaag tgggg
//
LOCUS       DACDHJ010000380          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.384, whole genome shotgun sequence.
ACCESSION   DACDHJ010000380 DACDHJ010000000
VERSION     DACDHJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.384"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>855)
                     /locus_tag="IV223_002697"
     CDS             complement(<1..>855)
                     /locus_tag="IV223_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8712926.1"
                     /translation="EDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEI
                     TFADQPAYVNSKGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPK
                     NIVSKLSFELTKFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTAT
                     DLTLGKYYFVEVPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKA
                     SYVNYKAPVIDKTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSET
                     SPYSVFKWG"
BASE COUNT          213 a          185 c          144 g          313 t
ORIGIN      
        1 ccccatttaa atacactgta aggagaagtt tcgcttttca ctccgtttac tgtgataccg
       61 tctgctccgc ctgcaatgtc tgttgggata tgaatcgttc cttgataatt tactgcgtca
      121 ccaatttgga aagaatgttc ttgtcctgtt ccatttgtta ctgttttatc gatcactggt
      181 gctttatagt ttacgtatga agcttttaag tcacttgtca cgccatcatt cgtaatttca
      241 aaggttaatt tattgtgcgc atcgttgcga gcgtctgcgc ctaataaata ttgatcggct
      301 gttggttctt tatctgatcc cactacaact tctgaaggaa cttccacaaa atagtatttt
      361 cctaacgtca aatcagtagc agtcagcttt ccttgatcat ccgatacaag atctcctaat
      421 tttgttcctt ttccaggttc gcccttgtat aactcaaatt ttgccccttt taaaggtgtt
      481 tgtttgcttt gagctgtgcc atcgtcacca aatttcgtta attcaaaact caacttagaa
      541 acaatatttt taggatataa gtgaatgtct tttaaaaaac ctgaacctgt attatcagtc
      601 attggcgcaa tcacaaccat tggttttgct ttttgtgtga ctaatccagc agcggattta
      661 ctttcataaa ccacatatac attccctttg gaattgacat aggctggttg atcagcaaaa
      721 gtgatctcac cattttcatc tacagctgaa gtaattttgt tggttgcatt cttgacgtac
      781 tcactgttag atcccttgtc ttctacatct ttaacgattt cgtccacctt cgcgttggtt
      841 agatcagaat cttca
//
LOCUS       DACDHJ010000381          839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.385, whole genome shotgun sequence.
ACCESSION   DACDHJ010000381 DACDHJ010000000
VERSION     DACDHJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.385"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..377
                     /locus_tag="IV223_002698"
     CDS             <1..377
                     /locus_tag="IV223_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8712927.1"
                     /translation="SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNIL
                     NKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLV
                     SLIISSFNILRRNPKELLIDNN"
     gene            507..>839
                     /locus_tag="IV223_002699"
     CDS             507..>839
                     /locus_tag="IV223_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8712928.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          289 a          125 c          159 g          266 t
ORIGIN      
        1 tatcattagg tatatcaaaa ttagaaattt ttggtcaatt cctaattgag atggtattta
       61 tatcaatccc cgcattcgtt ggttcctatt tcttagctca atatacagct gacaagcttg
      121 ggaataatat attgaataag gttactggtg atatagctaa acaaatagct aaacaatctg
      181 catctagcca attaggtggt ggggcagaag ctgaaggatt taacaagaca ttatcaagtt
      241 tagatatcaa tgtattacct aaattcatga tttatgtagt tatatttatg agtttagtac
      301 ttcttgtatc attgattatt tcttcattca atatattaag aaggaatcca aaagaattat
      361 taattgacaa taattaaaat tttcggtgct ttttagcacc aaaaatgaga gtgtaatata
      421 aaccgtgtaa gtaaccatca atcctaggcg ggtggttgct tcacggtttt tatttttcta
      481 aaataagatc aagaggtgat tacacgatgg ctagaaagaa aagaaatcct gatgctgaga
      541 agttagctga atccattttg aatacttatc aacccgaatc tgtagaggat atgcaagatg
      601 ttttaaaaga tgtgtttggt cccttatttg aaaaaatgct tcaaggagaa ttgaataacc
      661 atttagggta tgacgcccat tctaaagagc caaagaaaca tgacaatcga cgaaacggtt
      721 atggcaataa gacgcttaaa acgagctttg gtaaagtaaa cattgatgtt cccagagacc
      781 gggaggcttc ttttgaacct gagttgattc ctaatagaaa acgagatgtc tccgacatc
//
LOCUS       DACDHJ010000382          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.386, whole genome shotgun sequence.
ACCESSION   DACDHJ010000382 DACDHJ010000000
VERSION     DACDHJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.386"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>823
                     /locus_tag="IV223_002700"
     CDS             <1..>823
                     /locus_tag="IV223_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712929.1"
                     /translation="LNTVTIYAQSRTNRLGRRVVTLVEVDSKTGQEAISIIETKMDKK
                     SDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLLQYWGGEIKREPFKLSLLKRRGRNN
                     VGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQSEVGTTNRIYGSPVDSAYINNYPDV
                     YTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGCDKPKVSITVEFDKLTDSEEAKEFA
                     KIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSLSEKTLKLEAGDIQVAFYQQQAV"
BASE COUNT          300 a          109 c          162 g          252 t
ORIGIN      
        1 tttaaataca gtaactattt atgctcagag tcgtacaaat cgacttggaa gacgggtagt
       61 cactcttgtg gaagtggact ctaaaactgg tcaagaagcg atatcaatta ttgaaactaa
      121 aatggataaa aaatctgaca tacgacttta ttctgatatt acggctgttt ctagcacaat
      181 ctttgaagcg agaaatgttt taaattgtat tgctggtgaa caaggatcat tgcttcaata
      241 ttggggcgga gaaattaaac gtgaaccatt taagctttct ttgttaaagc gaagaggtcg
      301 caataatgtt ggaacgattc ggtatggaaa agatatgtct ggtttgaagg tcaaattaga
      361 ttggacaggt ataaaaacaa gaattattcc atatgctgat cctcaaagtg aagtaggtac
      421 gactaatcga atttatggtt cgccagtaga tagtgcctat attaataact atcctgatgt
      481 ctatacagaa catgttcagt ttacagaaga acaaggagta aaagacatta agagcttaaa
      541 taaaatagct aaaaattact ttaaaacgat caatcctggc tgtgataagc ctaaagtttc
      601 tattacggtt gaatttgata agttgactga tagtgaagaa gcgaaagaat ttgcgaagat
      661 tagaaattat ggtctgtttg atacatttaa aatatatcat aaaaaatata atatttatct
      721 tgaatcaaag gttagtggac tacaatacga ttctttatct gaaaaaactt tgaaattaga
      781 agcaggagac attcaagttg ctttttatca gcaacaagct gta
//
LOCUS       DACDHJ010000383          821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.387, whole genome shotgun sequence.
ACCESSION   DACDHJ010000383 DACDHJ010000000
VERSION     DACDHJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.387"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..295
                     /locus_tag="IV223_002701"
     CDS             <1..295
                     /locus_tag="IV223_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712930.1"
                     /translation="YTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVR
                     TRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(392..808)
                     /locus_tag="IV223_002702"
     CDS             complement(392..808)
                     /locus_tag="IV223_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8712931.1"
                     /translation="MIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRNGEGKII
                     GIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELLTDDTEK
                     TKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT          272 a          144 c          125 g          280 t
ORIGIN      
        1 ttatactgtt gaatttgaca gtgaactact taagcaattt ccacaagaag aacatttcat
       61 gtataaagaa gcaatttccc acaaagtgac agagtatttg gagcataatt tttttcgtat
      121 aaagccagtg cgtactcgtt cggcaaaagg aatatacgag atgaaagtca aaattaataa
      181 aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt tttatattag
      241 ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa aatagctgag
      301 cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa agaactaagc
      361 tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa agcacctttt
      421 ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga acaaatccta
      481 ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg atttggactg
      541 gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct attccttggc
      601 gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca aaatcagtga
      661 tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga gattcagatt
      721 gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa ccaacgctct
      781 tatataattc agaaatctca tcgatcataa gggaatcaat a
//
LOCUS       DACDHJ010000384          816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.388, whole genome shotgun sequence.
ACCESSION   DACDHJ010000384 DACDHJ010000000
VERSION     DACDHJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.388"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..806
                     /locus_tag="IV223_002703"
     CDS             <1..806
                     /locus_tag="IV223_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8712932.1"
                     /translation="YFAIQKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIK
                     DTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIF
                     NSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWM
                     RDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISAS
                     ETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT          325 a           98 c          146 g          247 t
ORIGIN      
        1 aatattttgc aattcagaaa tggattgaat tgaagttttc tattattcaa acttggatcg
       61 atttaaaata taatgccatt actacttgga ataatattaa acagtttttc aaagatactt
      121 ggaaaaatat taaagatacg gcatacaata cgtggatttc tataaaaaat accatgatta
      181 atacatggaa taacattaag gactctttct ggaatattgt tactggaatt gttaattctg
      241 ctgaaaatgc atggacaaat ctaaaaaatg gtgtttcaaa tgctattaat cgggtaaaag
      301 aaatctttaa ttcgttaagg gaagtcaatt tatttgaaat tggcaagaac atcattgatg
      361 gacttataaa tggtgtaaaa gaaaagtgga atgctttgaa agatactatc aaaggtattg
      421 caggtaatat caaaaattgg attaaaggag ctttagatat tcattctcca tcaagatgga
      481 tgcgtgatat ggttggtaaa aatattgttc aaggtattat cgttggtatt gataaagagc
      541 aaagtaagtt agatcaaacc atgacaaatt tagtaaaaac accgtctgtt caacctgtag
      601 taaatggaat aagtcaccta ccaattacac aaacaaagca aaatatatct gctagtgaaa
      661 caaaagaaat tcatttacat ttaaatgttt atggagattt acctgattca atggttcgga
      721 agattaccac aaaaattaaa acagaattga caagacaaat gaaacgagat gctggtgcag
      781 taggaggaac attatatgca acttaagaga ggacaa
//
LOCUS       DACDHJ010000385          816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.389, whole genome shotgun sequence.
ACCESSION   DACDHJ010000385 DACDHJ010000000
VERSION     DACDHJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.389"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..738
                     /gene="xerS"
                     /locus_tag="IV223_002704"
     CDS             <1..738
                     /gene="xerS"
                     /locus_tag="IV223_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8712933.1"
                     /translation="ALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          324 a          132 c          137 g          223 t
ORIGIN      
        1 gcattaaagg ttctatttaa atatctttcg aaaagttcca ataatccttt aggaaaacca
       61 tacttagaaa ataatcctat ggatcaagta gcaactatca cagataaaac tacgttagct
      121 gcccgtgcaa acgcaatcga gaaaaaatta tttctagacg aagatacaca aaattatcta
      181 gattatattg ccaatgaata taaaaaaact ttgagcaaac gagctttgat ctattatcaa
      241 cgagatgtag aaagagacct ggcgatcaat gcattgattt taggatctgg attaagactc
      301 tccgaggttg taaatattaa tttagaggac ctatctttag aaaagaataa tgtggtagta
      361 actagaaaag gaaataagag agacgcagta aatatagcag catttgcaat ggaatattta
      421 gctaattatc tagctattcg gaaagaacga tacaaagtaa cagaaaacga aaaagcttta
      481 tttctagcaa tttatcaagg agaagccaag cgtatatccg gtattgcaat cgaacggatg
      541 gtagcaaaat attcaaaagg attcagagtc caagtcagtc cacataaatt acgtcatact
      601 ttggctacta gactctatca acagacaaat tcattggtat taactgccca acaattggga
      661 catacaagta caaatacaac aactttatat actcatatag acaatgcagc tacaattgat
      721 gctttgaacg ctttgtaaat gtagtaaaaa atagtataat aatgaagcct gtctctaaaa
      781 ttagataaaa gcccaacttt aaaggcaagc ttcatt
//
LOCUS       DACDHJ010000386          804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.390, whole genome shotgun sequence.
ACCESSION   DACDHJ010000386 DACDHJ010000000
VERSION     DACDHJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.390"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            97..687
                     /locus_tag="IV223_002705"
     CDS             97..687
                     /locus_tag="IV223_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8712934.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          291 a          127 c          138 g          248 t
ORIGIN      
        1 aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat gttaaaactg
       61 ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat tacgtttcta
      121 ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa ccaaaaagag
      181 ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg aggattaaaa
      241 acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt tattatgatc
      301 ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga taaattgtta
      361 aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc tcagagcgga
      421 ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga atcggaaaat
      481 tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa tttgattact
      541 gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt ccattttgaa
      601 acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta tgattactat
      661 aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc cttatcaata
      721 gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat ttatcaaaga
      781 agatacactc tatttatatt cata
//
LOCUS       DACDHJ010000387          803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.391, whole genome shotgun sequence.
ACCESSION   DACDHJ010000387 DACDHJ010000000
VERSION     DACDHJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.391"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..378)
                     /locus_tag="IV223_002706"
     CDS             complement(<1..378)
                     /locus_tag="IV223_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8712935.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVA"
     gene            complement(685..>803)
                     /locus_tag="IV223_002707"
     CDS             complement(685..>803)
                     /locus_tag="IV223_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8712936.1"
                     /translation="TIKKDNNWIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT          260 a          141 c          101 g          301 t
ORIGIN      
        1 agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga catatttttc
       61 ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac gagataacag
      121 agctttgcta gaacattttc cattaatttc cagacatata tccaattcac tgacggttac
      181 tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat cgatcgtttt
      241 tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg actctgataa
      301 atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt ctttgcttaa
      361 aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt ccatgcattc
      421 accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt cagcgattct
      481 tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc gttttatttg
      541 gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat gcatttttat
      601 taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta caataataaa
      661 aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc tcaaaacaaa
      721 caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga tataaatcca
      781 attattgtct tttttaatcg taa
//
LOCUS       DACDHJ010000388          799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.392, whole genome shotgun sequence.
ACCESSION   DACDHJ010000388 DACDHJ010000000
VERSION     DACDHJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.392"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(48..317)
                     /locus_tag="IV223_002708"
     CDS             complement(48..317)
                     /locus_tag="IV223_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712937.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(314..694)
                     /locus_tag="IV223_002709"
     CDS             complement(314..694)
                     /locus_tag="IV223_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8712938.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT          259 a          174 c          118 g          248 t
ORIGIN      
        1 tgttagtaaa gacatgttat caacttctgt aaatcgaatc atcatcatta gtcaaacact
       61 tcttccacca cttctaccgc atacaccact tgaatagggt tatttaaaga aaatttcacc
      121 cgatattctt gattgttcaa actgacgata tagctatcct catcttcagc ataataggta
      181 tattcgttgc ctttgacttg aaaatatttt tggatcgctt gtctttgttg ccattcgcca
      241 atctttggaa aataggcgaa atgatagcca aataaaacgc ctatccctaa taaaacaatt
      301 cctgttacta taatcatctg gtttcctcct gtacctgttt atcttctatt gactcttttg
      361 tcggtaagca ataaagaacc agtaacacaa gagagccaat cgttgggaca aaatagaaaa
      421 aaataaatgc ccaatgaaaa cctgcgtctc gtaaacgtcg aacgctgaga gaaagagacg
      481 ggacaagaac tacgaaacaa tacacaatca atacgcctat caaacaacca attattgttt
      541 cagaagcatt caacgctcct gctagtccaa tcgctaagcc taggccaaac ccaatgaggg
      601 cattcattaa cgcaacgagc caatactctt ttcttgtggc tttccctttg taattaaagc
      661 tattctgcca aaatttttta taatttgtga tcatatcttt gtcctttcta atgtttattt
      721 ttgattctag tttcttctgt tttactaaca atgaaacttg ccataaaaaa ggtggccaat
      781 acaccaccag ctagaaatc
//
LOCUS       DACDHJ010000389          798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.393, whole genome shotgun sequence.
ACCESSION   DACDHJ010000389 DACDHJ010000000
VERSION     DACDHJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.393"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..628)
                     /locus_tag="IV223_002710"
     CDS             complement(<1..628)
                     /locus_tag="IV223_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8712939.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKL"
BASE COUNT          322 a          121 c          125 g          230 t
ORIGIN      
        1 ttaatttttt tataaaaata aaagcaccaa tcaatccgag aaataagaat aatccaatag
       61 tatagttgtt atagttatta tttaaaatat gtgtcagcat atcactagga gccataccac
      121 ttaattcttt tcccagtggg actttcaatt cttgtgctct agattgttga ataaaaggaa
      181 ttaagaatcc cgcacttaaa aaaaaggtga ggacggtagc ttttaaaaga cttaagagtc
      241 tagcgatttt ttggtcccaa aaataaaaag aaagaaacaa cgttcctcca ataaatacag
      301 aaaccatagc gacagaaagt aaatgagtat acactactag cgtcatgcca atagacaacc
      361 agtaccattt ttggtaatct ctaatataaa tttcgtaaca ccccattagt atcaaaggca
      421 aaaatgttaa agcgacagct tctcccaatg aagcacgatg gaatatgtca atggcacgat
      481 aggcggaaaa tgtataaata atcgaaaata acagcgaaat ataacgattg ttttttattt
      541 gtttcataga aaaataagaa acgaccattg ttaaaaaagt gataaaaaaa tagtagatat
      601 tataacttaa aacaagattt cccatcattt tataaaataa aaaagctgga taaagagtaa
      661 gccatggata aaaaatattc atcattgttc catgatgatc gaagttattg aagtttactg
      721 gagaactcca tacattatct agtcctatga ttcgagataa atgaaagtaa gtatcctgat
      781 tgacactatg atagattc
//
LOCUS       DACDHJ010000390          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.394, whole genome shotgun sequence.
ACCESSION   DACDHJ010000390 DACDHJ010000000
VERSION     DACDHJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.394"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IV223_002711"
     CDS             complement(<1..703)
                     /locus_tag="IV223_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAP8712940.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIK"
BASE COUNT          267 a          167 c          111 g          248 t
ORIGIN      
        1 atttaataaa gaactcttcc aataaggcac ttaaattatt ttcttcctgc tgtccacata
       61 aaaccattat tctctttttt gataatagtt tctgtttaaa aatctctact gatgacagta
      121 aatcatcctc ttgatatctt gtaattttac gatactctgg catggtctca acaaacggcg
      181 cacttaaatc cattaattga taattaatct gcacgggacc cttaacgcca tgatttaact
      241 ccaatattgc ttcatttatt tttctggtag cataccattc atcatcagaa tccttaacaa
      301 taggaatatc aacgttagaa cgcacataag ccccatacat atctacctga tcaatcatct
      361 gatcttccat ctgatataag cggcggtaat ctctatcagc agtaagaagt acaagttgga
      421 tgccattagc aaaagcatct ttagctgccg ccatataatt tgaagaggca gttgaagaag
      481 tacaacatac acacacgggc ttatcaatcg ccatagaaag tccattagca aaaaaggcag
      541 cactgcgctc atctacaacc gtataggttc taaaaaaact gtctgtttct acactatgca
      601 ccaattgaaa atctctacta cctggcgaga taactatatc tttaatatta tgctgtttta
      661 ataaagatac taaaatttga acatttttaa ctgacgatat cataattatc acctcttaac
      721 acaatggaaa ttccgttatt ttgcaataag gctaaatcct cagacaaaaa tcggttggtt
      781 gaatgagcta cac
//
LOCUS       DACDHJ010000391          781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.395, whole genome shotgun sequence.
ACCESSION   DACDHJ010000391 DACDHJ010000000
VERSION     DACDHJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.395"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..491)
                     /locus_tag="IV223_002712"
     CDS             complement(<1..491)
                     /locus_tag="IV223_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712941.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IER"
BASE COUNT          272 a          171 c           98 g          240 t
ORIGIN      
        1 tccctttcaa tgaaagaagc actaggaaaa gtatgattat aaccattcaa cctatctccc
       61 atgtaataat tagcaggtcc tggaaaataa aatatttgct tattatcact aacttctaac
      121 agcaacacta aatcttctcc gctggctaaa cgacttgtcc atctacgata tctccctatg
      181 ccttcgttca ttccaaatcc atcaaattca actattaacg tgtcttctat cacatgtaat
      241 tcttcattca attcatactc aagtctaaaa ggaaattctc catatgtaat ctctggcaat
      301 ggtggtgctg gagttctcca aataaataca ataactgtga atagaaaaaa tccaacgatc
      361 gatataaatg ttatgagacc tgctcgaagt aaaacccgat ataataaaaa tgctgtattt
      421 cgctttgcta tatctagaaa aataaaaagt actgcaatca taatgatagt aaaagttgta
      481 aaaaaacgca tttttgccct ccctttataa acatcgattc tcaaattcta aaccaaatct
      541 tggtagttca agttcaggta taacgtattc caaccataca tctcaattac tatttatgaa
      601 cttatgatta tcactgaata aatcactcca tccccttgtt aaaacttcta cattaattac
      661 acctggttgt tttcgactag ctaatccaat gaacttatca accatcctta tccaccaacc
      721 acgagaatct tgaccagaag taagaagagc agttctcaag gatgatcgag cagttccttc
      781 c
//
LOCUS       DACDHJ010000392          780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.396, whole genome shotgun sequence.
ACCESSION   DACDHJ010000392 DACDHJ010000000
VERSION     DACDHJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.396"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..327
                     /locus_tag="IV223_002713"
     CDS             4..327
                     /locus_tag="IV223_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712942.1"
                     /translation="MKKIVWIYSVNLKGMGLYGNSTTMPLRQAQKFQETIKTNLPSDV
                     TVDFISYDTSSTEIPKADLIVYNDIDSRYLSDDLKNNGIVIPFKDMISNNTREIEKKI
                     LLAIK"
     gene            complement(587..>780)
                     /locus_tag="IV223_002714"
     CDS             complement(587..>780)
                     /locus_tag="IV223_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HAP8712943.1"
                     /translation="AIIFFDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGT
                     KAGRMKNVVEAMMKLSNSI"
BASE COUNT          279 a          114 c          106 g          281 t
ORIGIN      
        1 caaatgaaaa agatagtatg gatatattct gtaaatttaa agggtatggg tctttatgga
       61 aatagtacaa ctatgccttt gagacaagca caaaaatttc aagaaacaat taaaacgaat
      121 ttgccgtctg atgttactgt tgattttata agctatgata cttcaagtac tgaaataccc
      181 aaggcagact tgattgtata taatgacata gactccagat atctaagtga tgacttaaaa
      241 aataatggta tagttatccc gtttaaagat atgatttcta ataatacacg tgaaatagag
      301 aaaaaaattt tgttagctat taaatagaat catatgtacc ctactaccaa attcaatttt
      361 ttatttaatg taaacgcttg catattttgt atacttatat tatagttaag ctatccattg
      421 aacgagattt cgttcaaaaa aaccagttgt aatatacaac tggttttttc catttgtttt
      481 ggtagctttt gccattcatc gataaaaact tatgccattt aaataaaaaa gttacacacg
      541 aaaaagcaat aggtcaaaaa acttaggaag tttctgacct ctttgtttat attgagttgg
      601 aaagcttcat catagcctca acgacatttt tcattctacc agctttagtt ccttctaata
      661 ttttatcaat atccatttta gtattagttt taaaattcaa ctgtttcatc ttatctatta
      721 aatttttcaa atctaagctt tctttgctga tagaatcctg atcaaagaaa attattgcat
//
LOCUS       DACDHJ010000393          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.397, whole genome shotgun sequence.
ACCESSION   DACDHJ010000393 DACDHJ010000000
VERSION     DACDHJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.397"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(541..726)
                     /locus_tag="IV223_002715"
     CDS             complement(541..726)
                     /locus_tag="IV223_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712944.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT          263 a          153 c           92 g          243 t
ORIGIN      
        1 atgcttttta tagaatagaa ctagtgttgt tttcatagac tctcctttct tcatatttcc
       61 aattaatcac aaattcacgg cattactaga aaaattaaag gggcgcagaa gtggaaaaat
      121 actcttcgac tttccctaca aaccaggcca tcttgtgttt aataactata ccaaatctaa
      181 ggtacctcta aatctaaatt ccatttgtgg cggtgacctc caatttttaa ctcttctatc
      241 tcccatccag tatacataac cttgatactt cgaccactta tatatcgaac acacaaatac
      301 ctatccattg caggagtact acctaactta gttgctactt gcatctaaat aacagctgaa
      361 aagataggac ttactcttag taaagtatct gaactattaa tttttaaact atcattttta
      421 tcctcaacta catgcacatc tgttgcaact tcactttctg tctcatccag ataaatgatt
      481 aaggaatcgg gaattgcaat atcttcagct gacgctggaa tagttgagct aaattagtca
      541 tcatatcttt attataaaat aaccaacagc aaaaaataca atgaaaaaca aacttggaac
      601 taatgtatca ttaataaatg ttgaaaaatt cagcaactca ttactgtatg attttagcag
      661 cccaaccaac atatacccta taaaagctaa taagatggtt aggaatcctt tagaattttt
      721 actcattcat agacacttcc ttaaataact t
//
LOCUS       DACDHJ010000394          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.398, whole genome shotgun sequence.
ACCESSION   DACDHJ010000394 DACDHJ010000000
VERSION     DACDHJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.398"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>741)
                     /locus_tag="IV223_002716"
     CDS             complement(<1..>741)
                     /locus_tag="IV223_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8712945.1"
                     /translation="IKRCIESIQKNLKKNDEIIVVDTGSTDNTIKIISSIENVKLFNY
                     EWKDDFADARNFGISKAKKDWIFFIDSDEIMSKGCLEQLNASINEAMAYSENNEKLVF
                     SPKAVNTDDSVFYNAGRIFANDGNIKYEGCVHEYPVVTDKSLTLVSLKLPRVIVRHDG
                     YEKEVRADKNKTVRNITLIKKILQEEPNNSQYYYYYYRDSKPLITIEEYEQGMLNFFE
                     KFPDSPYIDQVVRDLAYHYIQVGKNELA"
BASE COUNT          220 a          130 c           92 g          299 t
ORIGIN      
        1 tctgctaatt catttttacc aacttgtata taatgataag ctagatctct tactacttga
       61 tcaatgtaag gactatcggg aaatttttca aaaaaattta gcatcccttg ttcgtattct
      121 tcaatcgtaa tcaagggttt tgaatctctg tagtaataat aataatactg agagttgtta
      181 ggttcttcct gaaggatttt tttaattaaa gttatatttc ttactgtttt atttttgtct
      241 gctcgtacct ctttttcata accatcatgt cttactataa ctctaggaag ttttaaagaa
      301 acaagcgtta aacttttatc agtcacgaca ggatattcgt gtacacaccc ttcgtattta
      361 atgtttccat cattagcaaa tattcttcct gcattgtaga atacagaatc atccgtatta
      421 actgctttag gcgaaaaaac aagcttttca ttattttcag agtaggccat tgcttcattt
      481 atactagcat ttaattgctc taaacatcct tttgacatta tctcatcgga atcaataaaa
      541 aaaatccaat ctttttttgc ctttgaaata ccaaagtttc ttgcatctgc aaagtcatct
      601 ttccattcat aattgaatag ttttacattt tctatactag atataatttt tatagtatta
      661 tctgtagacc cggtatccac tactatgatt tcatcattct ttttcaaatt tttttgtata
      721 gattctatgc atcgcttgat t
//
LOCUS       DACDHJ010000395          740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.399, whole genome shotgun sequence.
ACCESSION   DACDHJ010000395 DACDHJ010000000
VERSION     DACDHJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.399"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..405
                     /locus_tag="IV223_002717"
     CDS             <1..405
                     /locus_tag="IV223_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8712946.1"
                     /translation="VLFYTQIATSEKGFNFDWQTTLFVSVIYFALLLGIVIAYFLLKL
                     LKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDDAPGLMLVGIAV
                     VFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            414..641
                     /locus_tag="IV223_002718"
     CDS             414..641
                     /locus_tag="IV223_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712947.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          246 a          117 c          133 g          244 t
ORIGIN      
        1 gtactttttt atactcagat cgctacaagc gaaaaaggat tcaattttga ttggcagacc
       61 acattatttg tttctgtcat ttactttgcc cttctcctag ggatcgttat tgcttatttc
      121 ttattgaaac tattgaaaaa aatcgaagat aaccaagctt tttccactca aagcttaaac
      181 atactaaaaa aaatcgaacg aaccattttg tttattggtt tcgccttttt tggcttactt
      241 cctaaattta tgaaagtagc ggatttagac gatgctccag gtttgatgtt ggtaggaatc
      301 gctgtcgtat tcattccttt tgcagtatac acattaacaa gcgtgctgga aaaactgctt
      361 gaacatgcga ttttacttaa gacagatcat gatttgacag tgtgaggtgc gacatggcaa
      421 ttatcgttaa tttagatgta gtattagctc aaaaaaaaat gagtgtgact gaattgagtg
      481 agaaagtggg cattacgatg gcaaacatct ctattctaaa aaatggaaaa gcaaaagcca
      541 tccgtttttc cactctcgaa aaaatctgcc aagttttgga gtgtcaacca ggtgatttgt
      601 tgacctataa aaatgactcg aatactatac cagaagaata aaataaagta gaacaaacag
      661 taaataaaaa ataatctgaa tgatagatgt gtgagacttt tagaagttat ttacgtctat
      721 tattcagatt ttttatttcg
//
LOCUS       DACDHJ010000396          737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.400, whole genome shotgun sequence.
ACCESSION   DACDHJ010000396 DACDHJ010000000
VERSION     DACDHJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.400"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..621
                     /locus_tag="IV223_002719"
     CDS             <1..621
                     /locus_tag="IV223_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8712948.1"
                     /translation="KKLGPNYKEQLKAVGQTEETYKKLFKQMLAFQYGLKANVKLTDK
                     DLDTAWKEFYPEVSTQIILFSTKEEADKAKKEANEGENFSKLVQAYGKNKTLKETDGK
                     MNFDSTNPEIPTEVKKAAFKLKNGEVSDIIPVTDPTTYQQSYYLVKMVKKQDKGSNKD
                     KYKSELEKIATEAKTLDTEFMDKTIRKVMKKDNVTIKDPYVKNIFK"
BASE COUNT          313 a          114 c          122 g          188 t
ORIGIN      
        1 aaaaaactcg gtccaaacta caaggaacaa ttaaaagcag ttggccaaac agaagagaca
       61 tataagaaac tctttaaaca gatgctggct ttccaatacg gtttgaaagc caatgtaaaa
      121 ttaacagata aagatctaga cactgcttgg aaagaattct atccagaagt tagtacacag
      181 atcattctat tttcgactaa agaagaagca gataaagcaa aaaaagaagc aaatgaagga
      241 gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata aaacattaaa ggaaactgat
      301 ggaaaaatga attttgattc aaccaatcca gagattccta ctgaagtaaa aaaagcagca
      361 tttaaactaa aaaacgggga agtttctgat atcattcctg taacagatcc aacgacttat
      421 cagcaaagct attatctagt aaaaatggta aaaaaacagg ataaaggttc taataaagac
      481 aaatacaaat ctgagctaga aaaaattgct accgaagcaa aaactcttga tacggaattc
      541 atggataaaa caatccgtaa agtgatgaaa aaagacaatg taactattaa agatccttat
      601 gtaaaaaata tattcaaata aacaaatttg acccaattgg ggatgtggca aaatttgaga
      661 agtgattttt ggaaattcct tcttattttt gaggcaaacc acttttgcca caacttcatc
      721 ggggcctttt tttattt
//
LOCUS       DACDHJ010000397          737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.401, whole genome shotgun sequence.
ACCESSION   DACDHJ010000397 DACDHJ010000000
VERSION     DACDHJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.401"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(111..719)
                     /locus_tag="IV223_002720"
     CDS             complement(111..719)
                     /locus_tag="IV223_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8712949.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          187 a          136 c          149 g          265 t
ORIGIN      
        1 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
       61 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
      121 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
      181 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
      241 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
      301 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
      361 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
      421 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
      481 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
      541 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
      601 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
      661 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
      721 ttaaactcct ttttttc
//
LOCUS       DACDHJ010000398          721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.402, whole genome shotgun sequence.
ACCESSION   DACDHJ010000398 DACDHJ010000000
VERSION     DACDHJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.402"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..235)
                     /locus_tag="IV223_002721"
     CDS             complement(<1..235)
                     /locus_tag="IV223_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8712950.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTL"
     gene            complement(222..551)
                     /locus_tag="IV223_002722"
     CDS             complement(222..551)
                     /locus_tag="IV223_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712951.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          220 a          155 c           85 g          261 t
ORIGIN      
        1 taagcgtcat tttaaaccat tcattacctt gatcatcttc aggtagcaag ccgaatccaa
       61 ttggattatc agtcaatttt tgatcgtata aatcatcctc gtctaatgct aaaacatcta
      121 atcctttaca atttccgcct cttctaaacg tcacaaatcc tttgtaatcc caacgttgga
      181 aagaaacctt aatcgtatgt gttccccatc ggctattcgg atcataattt ttcatttgtt
      241 gtaacctcct cataatcagt tagtactata aaggtatctt tgtttttatc caaaaaatct
      301 aataaatctt ttgtagaaga tatctcttca tcaacttcac cataataaat accttcgata
      361 attgttgcta tatctgaaaa actaattttt actggacatt tatcgtctac cgcattcaat
      421 accgacaaaa ttgcttcttt ttctgcaatc tctcgtttga atttctttgg agctaatcgc
      481 atgacactca agtcattttc aagcttttca atccatgcag ttttactcat tttcgcaaat
      541 ttttgaatca ttcctctacc acctttgcta tctactaaat tagttttact tcataacgaa
      601 tatttttatc gtttttcgtt cactcaaaaa tatataaaat aaatcgactc gtttatggcg
      661 tccgaaacga gaaaccctag tagaaaacgg taaatgctcg attgatccaa agatcaacgt
      721 c
//
LOCUS       DACDHJ010000399          715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.404, whole genome shotgun sequence.
ACCESSION   DACDHJ010000399 DACDHJ010000000
VERSION     DACDHJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.404"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..267
                     /locus_tag="IV223_002723"
     CDS             <1..267
                     /locus_tag="IV223_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8712952.1"
                     /translation="KELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQ
                     RKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            339..>715
                     /locus_tag="IV223_002724"
     CDS             339..>715
                     /locus_tag="IV223_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8712953.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKN"
BASE COUNT          306 a           91 c          109 g          209 t
ORIGIN      
        1 aaagaattga gtagtgcatc ggttccattt gaatttgacg ttcatgcgat gattttttca
       61 gaggacgctc cgtctttgga aactaaattg cataatcatt ttagaaaaca agaagttaat
      121 aaaattaatc aaagaaaaga atttttcaaa gtttcactag acgaaataga gaaagttgtt
      181 ttagaaaatt ataatggaac agtaacattt acaaaattag ctaaagctga acaatataga
      241 cgatctttag aattgtcaaa agattaaata tataaaaagc cttcgggctt tttcttttaa
      301 caattaatag gaacgtatat tcgaaaggaa tagaaataat gtggttagaa aaaataaatg
      361 acaaaaaatt taagtacacc gaacgttata cggatccctt aactgaaaaa aagagaaaag
      421 tgtccgttac tttatctagc aattcacgcc aagcttggaa tcaagctaac ttacttttga
      481 atgaaaaaat cgcagaaaaa ataaaacgaa atgaagaatt accactaact tttggagaat
      541 taaaaactaa atgggatgaa aaatacaagc caactgtaaa agaaagttcc tatcgaacaa
      601 ctcaggttta tctatcatta atttcaaaat atataaaaga tgatgtactt gtaaaaaacg
      661 ttaatagtaa tcttatacaa gatatgctag attactacta ttttgaaaaa aattt
//
LOCUS       DACDHJ010000400          707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.405, whole genome shotgun sequence.
ACCESSION   DACDHJ010000400 DACDHJ010000000
VERSION     DACDHJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.405"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(636..>707)
                     /locus_tag="IV223_002725"
     CDS             complement(636..>707)
                     /locus_tag="IV223_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8712954.1"
                     /translation="LKKIENLPLQDMAKIDQIIQYMF"
BASE COUNT          214 a          108 c          106 g          279 t
ORIGIN      
        1 taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg
       61 acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa
      121 actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca
      181 aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa
      241 actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga
      301 tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg
      361 tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag
      421 atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc
      481 tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat
      541 cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta
      601 gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg tataatctga
      661 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgag
//
LOCUS       DACDHJ010000401          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.406, whole genome shotgun sequence.
ACCESSION   DACDHJ010000401 DACDHJ010000000
VERSION     DACDHJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.406"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>702
                     /locus_tag="IV223_002726"
     CDS             <1..>702
                     /locus_tag="IV223_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8712955.1"
                     /translation="KGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQ
                     QELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMG
                     TQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSC
                     VRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFG
                     ETYVLKMPAINILTLA"
BASE COUNT          262 a           95 c          135 g          210 t
ORIGIN      
        1 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
       61 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
      121 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
      181 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
      241 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
      301 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
      361 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
      421 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
      481 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
      541 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
      601 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
      661 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ct
//
LOCUS       DACDHJ010000402          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.407, whole genome shotgun sequence.
ACCESSION   DACDHJ010000402 DACDHJ010000000
VERSION     DACDHJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.407"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..>697
                     /locus_tag="IV223_002727"
     CDS             80..>697
                     /locus_tag="IV223_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8712956.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILL"
BASE COUNT          264 a           75 c          135 g          223 t
ORIGIN      
        1 aaaatttagg gttgactaaa atataatttt tgtataccat atgatcgtga taatcattat
       61 catttagaag gagaaagtta tgttagaagt gaaaaagctt aatgtttgtt ataacgattt
      121 tttagcattg aacgagattt ctttaaaaat acaagaaggt gctttaacag gtattattgg
      181 tccaaatggt gctggaaaat ccacattgct taaagcaatg ttaaatatta tccctcatca
      241 gggagaggtc tccattaata atgaagatat caataaaaaa ctggtgaaga ttgcatatgt
      301 agagcaaaaa gctgatattg attttacttt tccaataaaa gtaaaagaat gtgtatctat
      361 gggaacatat gccggaatga aagtttttca aagaattaaa aatgcagaat ggcaaagagt
      421 ttcaaaggct ttggaaaagg tcgatatggg aaaatattct aatcatcaaa taggagaatt
      481 atctggagga caatttcaac gtgtactttt agcaagatgt cttgctcaaa atgcggattt
      541 catattttta gatgaaccat ttgtagggat agacttagtt agtgaacgaa taattatgga
      601 tacgttaaaa aaattgaaac atcagggaaa aacagtgcta attgtgcatc atgatttaag
      661 caaggtaaag aagtattttg ataatattat tcttttg
//
LOCUS       DACDHJ010000403          686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.408, whole genome shotgun sequence.
ACCESSION   DACDHJ010000403 DACDHJ010000000
VERSION     DACDHJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.408"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(435..635)
                     /locus_tag="IV223_002728"
     CDS             complement(435..635)
                     /locus_tag="IV223_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8712957.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          203 a          142 c           92 g          249 t
ORIGIN      
        1 gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg tattccttca
       61 aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact taaaaattta
      121 gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat tgacagacca
      181 atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt cattttcaag
      241 acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta ttcgtatttt
      301 cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta ttcagcccat
      361 tcaacgacca atgaattttt tcgtacaaaa aaaccgtctt ttctttcgaa aagacggtta
      421 aaagtcaatt tttattaagc tttaacaatg tttgttgctt gagggccacg ttgaccatct
      481 tcaacatcgt aagacactgc ttggccttct tctaaagttt tgaagccgtc accttggata
      541 gctgaaaaat gtgcaaatac atcatttcca tcttcaccag tgataaagcc aaaacctttg
      601 tctgcgttga accattttac tgtaccgtta ttcataaata aattcctcct gatacgtatc
      661 aatgtacgtt ttttcgttgc aataaa
//
LOCUS       DACDHJ010000404          686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.409, whole genome shotgun sequence.
ACCESSION   DACDHJ010000404 DACDHJ010000000
VERSION     DACDHJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.409"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            16..501
                     /locus_tag="IV223_002729"
     CDS             16..501
                     /locus_tag="IV223_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712958.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          211 a          129 c          113 g          233 t
ORIGIN      
        1 ttaggaggag ctatcatgca taaagggatt tacgcaggct tgttgggaac tgtaatactt
       61 ttttctgcct gtggatataa aacaaagaat tctgttaaag aatcttcagc agccatttca
      121 acaactgagg ttggttcaac taaaagtact tctactactt ccattactaa tacttctact
      181 ggtaatgtag attcgcaaca tagccaatca agtattcagt cgtttagcat ttcttcaaca
      241 tctgaaatgc aagaaagcac tgtaccttct cttccattag aaaactctga caaagcactt
      301 ctatttctta ttgcccaccg ttccgaatta caaagtgacg atattgttat tagcttttat
      361 caaaaaattg atcgagatta tttgttcaca gcttccagca aacaaatccg ttcccaagga
      421 ggatctggaa gcgttggttt ttatcgagtg tcacccgaag gatcgattac gatgacagat
      481 gcaaatggaa caccattttg attagatgat attatacgga tttacagtac tagcaagtca
      541 ttattggtat cattttttct gagaaagtta tacggagtat aaataagcat ttgcattaac
      601 ctaataattc gttacagcta tttctcttat gaatatactc acttctctac ttgctctctt
      661 gataaatttt tggtatctac ttcatt
//
LOCUS       DACDHJ010000405          675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.410, whole genome shotgun sequence.
ACCESSION   DACDHJ010000405 DACDHJ010000000
VERSION     DACDHJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.410"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..225)
                     /locus_tag="IV223_002730"
     CDS             complement(<1..225)
                     /locus_tag="IV223_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8712959.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFH"
     gene            complement(243..563)
                     /locus_tag="IV223_002731"
     CDS             complement(243..563)
                     /locus_tag="IV223_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8712960.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          228 a          124 c           92 g          231 t
ORIGIN      
        1 gtgaaaagtt gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg
       61 tgcaagttca tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa
      121 aaaaatgatt ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac
      181 tttcaaattg tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc
      241 aactattcat tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta
      301 gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta
      361 ggggtttctc tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca
      421 atccgactta attcatctga attaggagta ttatttttcc actttgctag ataaccgtta
      481 gaatatccaa aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct
      541 tttattattt cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt
      601 ttagaaaaat acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg
      661 taaacagatt taaca
//
LOCUS       DACDHJ010000406          654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.411, whole genome shotgun sequence.
ACCESSION   DACDHJ010000406 DACDHJ010000000
VERSION     DACDHJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.411"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            59..205
                     /locus_tag="IV223_002732"
                     /pseudo
     CDS             59..205
                     /locus_tag="IV223_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="IV223_002733"
     CDS             282..611
                     /locus_tag="IV223_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8712961.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          251 a           80 c          120 g          203 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gcta
//
LOCUS       DACDHJ010000407          654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.412, whole genome shotgun sequence.
ACCESSION   DACDHJ010000407 DACDHJ010000000
VERSION     DACDHJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.412"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            31..>654
                     /locus_tag="IV223_002734"
     CDS             31..>654
                     /locus_tag="IV223_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8712962.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDE"
BASE COUNT          251 a          100 c          115 g          188 t
ORIGIN      
        1 tcaaaaatga aattttgaga gggattaaat atgacaatta aaactagtct ccaaaatcaa
       61 gcttatcaat ctattcgtca gcaaattatc tacgctgact tagaacctgg aaaaaaagta
      121 tcagaaaaaa aattggaaga aacgctttct atcggtcgta ctccaatacg tgaagcattg
      181 attcaactcc gtcatcaagg acttgttgat acaattcctc aaagtggaaa ttatatctca
      241 ttaatcgact tagatttagc aaaaaatgct cgttttgtca gagaacattt agaacgacaa
      301 attatgctag agtgttgtgc caagatgaat aatcaaaaga aacaagtttt agaaactata
      361 attgcagaac aagaaaaagc tgttagtcaa aagaatgctc gtagtttttt tcataatgat
      421 aatttatttc ataaagcttg ttttgaaatt gcagaacggg aagaagtatg gcaatggttg
      481 gaagataata atacgcactt ggaacgattt cgttggttac gagtaaaaac ggaaggatta
      541 aaatgggaaa aaattatgga acaacatcat caactattta aagccttgac catgcaagac
      601 cctgaagaag ctaacttctt aacatccttg catcttcatt taatgcttga tgaa
//
LOCUS       DACDHJ010000408          652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.413, whole genome shotgun sequence.
ACCESSION   DACDHJ010000408 DACDHJ010000000
VERSION     DACDHJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.413"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(225..>652)
                     /locus_tag="IV223_002735"
     CDS             complement(225..>652)
                     /locus_tag="IV223_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8712963.1"
                     /translation="NLPQFLTYSYSNVNLVPFSTFSFENTTVLRETTYNLIVFFPFGV
                     LLNVNFKRLSFSKKLGIIFLVSFLAELIQFLFGIGVADITDLITNTTGGLIGLWAYQL
                     LNKHLSTNKLDRLAIILGYILFLFLIVAFLFLTRTVRLQ"
BASE COUNT          252 a           96 c          121 g          183 t
ORIGIN      
        1 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
       61 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      121 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      181 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      241 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      301 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      361 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      421 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      481 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      541 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      601 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aa
//
LOCUS       DACDHJ010000409          645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.414, whole genome shotgun sequence.
ACCESSION   DACDHJ010000409 DACDHJ010000000
VERSION     DACDHJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.414"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          240 a           92 c          112 g          201 t
ORIGIN      
        1 gttgatttga tattaacaag cgtatatgaa tgtatataca aataaaaaag gggagggaga
       61 agttaaagaa aattcagaga agtggggcac tcagtagtat taacaattta aaaggaattt
      121 cttaaagaaa atgggttgca tcctggagat acaatttttt tagataaaga aaaaattcaa
      181 gcaacaattc aaaaaaatct taactgctga gattgatgta gcaatatctc aatcatcaac
      241 aaagtacgat cctgcctttc aaaagcttat tgataaatgt ttattcatta taaaaataaa
      301 agaaaaattt atgtcagctt ttttcatcaa agtggtaatg ataattcgtc tattaattgt
      361 attttttagg aaatatggta taattgtttt aacgaaaagg taattcatga tacaccaaaa
      421 accactagct ggcgggctag tggttttctt ttacgttcta ttcagattaa cgggtaggct
      481 tactgtcttt tttggcaata attttgcaat caagccattt acagatgtaa tgcgcggata
      541 cttctgctaa gattgcaaaa aacatatcga aaaggtaatt cataatacaa cctcctcccc
      601 ttgccataat aggccggggt agcagacagc ataaatatta tacca
//
LOCUS       DACDHJ010000410          624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.415, whole genome shotgun sequence.
ACCESSION   DACDHJ010000410 DACDHJ010000000
VERSION     DACDHJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.415"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            61..429
                     /locus_tag="IV223_002736"
     CDS             61..429
                     /locus_tag="IV223_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8712964.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          225 a          106 c           98 g          195 t
ORIGIN      
        1 atacataact attgtttttc tggtaaaata aatcaaacca cttaatggag gcgttttaga
       61 atggaaaaat ttcagccgat catcaatcag atcaaagaaa aagaacatca acaaaagatc
      121 aaagaccttt ttgctaagat aacagaaacg tatccccagc tagacacaac tgtaaaatgg
      181 aaccaaccta tgtttactga ccatggtacc tttattattg catttagttt tgctaaaaat
      241 catttttcta tcgcaccgga aaaagcagcg attcgtgccc ttgaaaaaaa tatccaagaa
      301 gcaggctacg tatatacgga taacgtcatc aaagttcctt ggaaaagtgt aatcaactgg
      361 gaattgattg aacaattgat ttcatttaat attgaagata aaaaaggtca tgataaattt
      421 tggcgataaa gctttatacg aataaacctt ttactggcga ggaaactatt catcagctta
      481 tacaggtttt gttcaatcct atccttttta tcatccctgg ttaaacactt aattgacaaa
      541 gtatctgcta tttttataag gagtctttct tgtgctgaaa aaaatcactg attgaatggc
      601 tttgaatttt ccacttagta aaat
//
LOCUS       DACDHJ010000411          612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.416, whole genome shotgun sequence.
ACCESSION   DACDHJ010000411 DACDHJ010000000
VERSION     DACDHJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.416"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>612)
                     /locus_tag="IV223_002737"
     CDS             complement(<1..>612)
                     /locus_tag="IV223_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8712965.1"
                     /translation="YKGVQAQFIKGKLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVK
                     EKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAK
                     KYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQL
                     FTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKT"
BASE COUNT          176 a          128 c          124 g          184 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 aggatcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 acagaattca ttgatttgaa ctcatccata cacaacaccg tcggaaggaa gttcatacgt
      241 ggctggtaat gcttcgttgc ttcacgcaag atccgtgtca ccgtcccatc cgagacgaag
      301 tacttcttag cgattagttt acgtgattga ttttcgtaca gttccatgag aatcgcctgc
      361 ttcagttcct ttgttagttg atgatgttca tcaactaagt ccgttttcgc agaaaaggtc
      421 cttccacaat catggcagag aaaacgggta cgttttaatt ctaaaatagt taacttttct
      481 ttgactttca acatctgagt gcgtgtcgta taggaaccgt tgcggataat acgaaccgat
      541 cgacagtgtt cacaatagga agaagagtca tccaattttc ctttaataaa ctgcgcttga
      601 acgcctttat at
//
LOCUS       DACDHJ010000412          600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.417, whole genome shotgun sequence.
ACCESSION   DACDHJ010000412 DACDHJ010000000
VERSION     DACDHJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.417"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>600
                     /locus_tag="IV223_002738"
     CDS             2..>600
                     /locus_tag="IV223_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8712966.1"
                     /translation="MILGAGIYGLAVTGINIPSKLADGGVTGIALLLHNLFGFAPSVT
                     SLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFAVDSLLINSLMTG
                     ILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGILVVSLLYLDLTH
                     FLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETF"
BASE COUNT          147 a          107 c          113 g          233 t
ORIGIN      
        1 aatcatttta ggcgcaggaa tctacggatt agctgtgaca ggaatcaata ttccatctaa
       61 gctggcagat ggtggggtta ctggtattgc tttgcttttg cataatcttt ttggattcgc
      121 tccttctgtc acttcactgc tttttaattt acctttattg cttatctcat tgtttctttt
      181 tggaaaacac gcttttctcc ggacgatcgt cggaacattt gcgctagttt tctttttaca
      241 tttatgggag agccttcata tacattttgc tgtagatagt ctactgatca atagtttgat
      301 gacaggtatt ctttctggca ttggttgcgg tctcgtcttt cgattcggcg gaagcacagg
      361 cggaacagat atcatttatc aagccattga aaaatatttt catattaaga ttggcaaaag
      421 tcttttcttg attacttttg gtattttagt tgtctccttg ctttatttgg atctcacaca
      481 ttttctgtat acattactta gctgttctat tctatcttat acattgaata aagtgaagta
      541 tctggaaatt ggatctgttt tgccccaatt ggaagcaaaa cagactacag aaacatttga
//
LOCUS       DACDHJ010000413          584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.418, whole genome shotgun sequence.
ACCESSION   DACDHJ010000413 DACDHJ010000000
VERSION     DACDHJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.418"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            137..>584
                     /locus_tag="IV223_002739"
     CDS             137..>584
                     /locus_tag="IV223_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP8712967.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          209 a           90 c          122 g          163 t
ORIGIN      
        1 ggtatcccat tttgcagcaa aatcatgttg ataaagtgac aagaagactg atgagatata
       61 ttatccaaat tgagaagata attaatcgga ttcttgagta taccctactt tttaagaaag
      121 tagggtatac aagcgtatga ataaagacat aactttccct gtgatggacg ttcggcttgt
      181 tccaattacc aaaatacagg cgaacgatta taatcccaat cgagtagcgt ctccggaaat
      241 gaacttattg gagctttcta tcattgaaga tggctttact caaccattag tttgttatta
      301 caaggaaaaa gaagagaagt atatcttggt tgatgtgctt cataggtatc gtgttgctaa
      361 ggaaagactc aacttaaaag ttgtaccggt aacagttatt gataaaccca tagaagaacg
      421 tatggcttca acaattcgac acaatcgtgc aagagggacc catgaaataa gaggaatgga
      481 aattgtcatc caaagattag taaaaaatgg atggtcagac aagagaatat ccaaagaatt
      541 aggcatggac attgaagaaa tttttcgctt taagcaagtg tctg
//
LOCUS       DACDHJ010000414          578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.419, whole genome shotgun sequence.
ACCESSION   DACDHJ010000414 DACDHJ010000000
VERSION     DACDHJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.419"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(55..140)
                     /locus_tag="IV223_002740"
     tRNA            complement(55..140)
                     /locus_tag="IV223_002740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Leu,seq:taa)
     gene            complement(157..228)
                     /locus_tag="IV223_002741"
     tRNA            complement(157..228)
                     /locus_tag="IV223_002741"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Gly,seq:gcc)
     gene            complement(240..312)
                     /locus_tag="IV223_002742"
     tRNA            complement(240..312)
                     /locus_tag="IV223_002742"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Thr,seq:tgt)
     gene            complement(330..411)
                     /locus_tag="IV223_002743"
     tRNA            complement(330..411)
                     /locus_tag="IV223_002743"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Leu,seq:tag)
     gene            complement(419..491)
                     /locus_tag="IV223_002744"
     tRNA            complement(419..491)
                     /locus_tag="IV223_002744"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Lys,seq:ttt)
     gene            complement(495..567)
                     /locus_tag="IV223_002745"
     tRNA            complement(495..567)
                     /locus_tag="IV223_002745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Val,seq:tac)
BASE COUNT          147 a          163 c          138 g          130 t
ORIGIN      
        1 tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt gaaatgccga
       61 ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc ctgtgcgtct
      121 gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg ttcgaacccg
      181 cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt gtttaaagat
      241 gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct ctaccaactg
      301 agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac ccccacgccg
      361 taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg caaaaatatg
      421 agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc taccaactga
      481 gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
      541 atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDHJ010000415          562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.420, whole genome shotgun sequence.
ACCESSION   DACDHJ010000415 DACDHJ010000000
VERSION     DACDHJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.420"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..394
                     /locus_tag="IV223_002746"
     CDS             <1..394
                     /locus_tag="IV223_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8712968.1"
                     /translation="NYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLTNSN
                     EGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDTEIG
                     KILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            388..>562
                     /locus_tag="IV223_002747"
     CDS             388..>562
                     /locus_tag="IV223_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8712969.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITY"
BASE COUNT          215 a           87 c           94 g          166 t
ORIGIN      
        1 aaattacaat gagaaatcta tacaacatca atttgatgcc tactgtaaaa aagtattacg
       61 aaacgaaact aagtctattc gaaaacgaaa cattaacata acagataatg aggaaccatt
      121 gacgaattca aatgaaggta agtactccca aactcatttt gatgaacaag atgtctattt
      181 cttatttgga atggaaatat taatttcaga tcaaaagtta tcggcagcca ttgatcattt
      241 atcggaaata agaagaaagg ttatcctact ttattatttt gcaggtttta atgatactga
      301 aatcggtaaa atcctcaata tgagtacaag tggtatctgg tatcaacgaa agaaggcagt
      361 tgaacaatta aaaatggggt atgacttatg gtagacaacc ccaaaaaacg gcagtatcca
      421 ttagttcctc tatccattat ccaatctgct ctagaagaag atccaatttc aatcaagatg
      481 atactcaaac attatcatag ttatatagct aagttatgtt tgacaaatgg atttaatgag
      541 gcggggcaat ttattaccta tg
//
LOCUS       DACDHJ010000416          561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.421, whole genome shotgun sequence.
ACCESSION   DACDHJ010000416 DACDHJ010000000
VERSION     DACDHJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.421"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          224 a          119 c           61 g          157 t
ORIGIN      
        1 gaataaacag aataacattt tcaaaacatt ctgtagttaa tctaatctcc cctgtcgttt
       61 cattcacaga gtaaatccac caactaccaa acacattaga caaaggattt agccaaacat
      121 atctatccat cagtgttcta aaaagaatca tatttgtaaa aaacacaagc aaaaacattt
      181 ttctaaatga gcttgatttt ctgaaatacc gaatccagac aagaaaagat tttttcaaac
      241 ctaatcctga tttctcctca gccatcgcaa acaagtataa aaacatagcc aaaactgcaa
      301 ttaaaatcga aacccaaata tttgatacaa tgcagataac acattaacca aaatagaaga
      361 tatgattttc ttcataaact ctcctttcat catgcgctaa atgagtacca tcaagaaggt
      421 ttcgtaaaag gaggtaccca ctctaacatg aataacctcg gccagcgaaa ataaaactca
      481 acagaattcc tatctctatg ctgctataat ttcaacaaat ttacatgtac cagttactaa
      541 tctaatataa ttttttcagc a
//
LOCUS       DACDHJ010000417          554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.422, whole genome shotgun sequence.
ACCESSION   DACDHJ010000417 DACDHJ010000000
VERSION     DACDHJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.422"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>554
                     /locus_tag="IV223_002748"
     CDS             <1..>554
                     /locus_tag="IV223_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8712970.1"
                     /translation="SYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNG
                     QVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADV
                     VTYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDD
                     VANAAILKSDKEGHFEITGLTEGE"
BASE COUNT          213 a           84 c          117 g          140 t
ORIGIN      
        1 tcttatgtcg ctgctaaagc aaatatcaaa gtaacaaata aagatggatc tgtggtatta
       61 aaagaaaata cagattataa aatccaaaat tcagaaaatg gctttgtgat cgattttatc
      121 gtgaataatg gccaagtaag tgatactgta gcaagtttac acggacaaga tcttcaaatg
      181 acttataaca tgtatgtaaa tgacagcgca gctgttgcca atccattaac aaatagcgtg
      241 gatttcgttt ataacaacaa tccatttaat caggaagaac accacgaaaa aacgaaagct
      301 gacgttgtga cttatggtgc aaaattctta aaagtcgata gcggactatt tggaacagga
      361 atcaaagcaa caccgcttga aggcgcagaa tttgctgcta aaaacgcaga aggaaaatat
      421 tatggtggat tagttgatac ggataaagac ggtgtcaaag aagcagtatg ggtagacgat
      481 gtagcaaatg cagcgatcct aaaatcagat aaagaaggtc actttgaaat cactggttta
      541 actgaaggtg aata
//
LOCUS       DACDHJ010000418          549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.423, whole genome shotgun sequence.
ACCESSION   DACDHJ010000418 DACDHJ010000000
VERSION     DACDHJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.423"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV223_002749"
     CDS             <1..390
                     /locus_tag="IV223_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8712971.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          205 a           95 c           83 g          166 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccatcctaaa acataagcta tttgtttact tctgtagaat
      481 atatactttg ttgttagata agttgcacat gtttatagaa tctaaataat acccgtggtg
      541 ctagttaat
//
LOCUS       DACDHJ010000419          541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.424, whole genome shotgun sequence.
ACCESSION   DACDHJ010000419 DACDHJ010000000
VERSION     DACDHJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.424"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>541)
                     /locus_tag="IV223_002750"
     CDS             complement(<1..>541)
                     /locus_tag="IV223_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8712972.1"
                     /translation="NPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVTLH
                     IQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRKVT
                     DELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFT
                     HQIKDNQVNVLNIITKKLV"
BASE COUNT          167 a           92 c           70 g          212 t
ORIGIN      
        1 tttaccaatt ttttggtaat tatatttaaa acgttcactt gattatcctt tatttggtgg
       61 gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag catcttctgt ttgttcattt
      121 aactgtagat actgttttac ttttctgaca tctgccccta acatcaaagg actaacaaca
      181 ataacaggaa aatttgtatt ttcaatcgga atcgtgctta taacaagttc atcagtgact
      241 tttctatctt gtagttcatt gatgcttagt acatcttcta tctcaaccat atctgcaaaa
      301 ccgttattta ttctttgctc taataatttt gatgttccat atccgctact acaaactaat
      361 atcactttcg ttttgtcagg tttgtaacga tccaaaagtg attgaatatg gagtgttacg
      421 taagcgattt catcttcatt aaaatgtata cagaatctct cttcgatttc ctctaataaa
      481 tctacactta tcataaatga ccttttaaaa gaatatttga ttttgtccgt ataagggtta
      541 t
//
LOCUS       DACDHJ010000420          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.425, whole genome shotgun sequence.
ACCESSION   DACDHJ010000420 DACDHJ010000000
VERSION     DACDHJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.425"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(67..288)
                     /locus_tag="IV223_002751"
     CDS             complement(67..288)
                     /locus_tag="IV223_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8712973.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          185 a           88 c          102 g          159 t
ORIGIN      
        1 atttttttgt tttcattaag acgtattacc taagagttcc atgactttag tctggtagcg
       61 tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct tttttctata
      121 gtatataaat ttttgcatat agataaagat caagataccg ataacggaag ctagattgta
      181 atctatttta gtgacagtca ggtatagcca agtaactaga agatcagtgg aacctgcacc
      241 ataaaagttt gagtttgtcg gtccgttgac agttaaaaga aaaatcacgt tgaagttatt
      301 gatatttccg attacgccgc ttcgatttgg tcacgatcca aattttggat aatccctgta
      361 gaaattagca tagtagctgg gataccaatt cacatattga caaagataac agagactttt
      421 acataaaacg gatcaatcag gaaaggaatt gctcgatcca gccccagttt tccaacaatg
      481 tgttaactgg cctagcgtca ttcagcaaat tatgcatgat gaggagcgaa atga
//
LOCUS       DACDHJ010000421          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.426, whole genome shotgun sequence.
ACCESSION   DACDHJ010000421 DACDHJ010000000
VERSION     DACDHJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.426"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>534
                     /locus_tag="IV223_002752"
     CDS             <1..>534
                     /locus_tag="IV223_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8712974.1"
                     /translation="TPDVIIGRAERTFSCSVSTLYRRFKTGEFNVLHLPMQGKRKPNG
                     YKEKRGKQAFKRNISERKKDYVVFEEEFGHLEGDTIVGIHHKSAVITLVERLSKAIIA
                     LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPG
                     TPSQRGLNEHSNGLLRK"
BASE COUNT          184 a          103 c          106 g          141 t
ORIGIN      
        1 gaccccagac gtcattattg gccgtgcaga gcgaaccttc tcttgttcag taagtaccct
       61 ctatcgccgg tttaagacgg gagaattcaa tgttttacat ttaccgatgc aaggaaaacg
      121 aaaaccaaat ggttataagg aaaaacgtgg aaaacaggca ttcaaaagaa atatttctga
      181 acgtaaaaaa gattatgtcg ttttcgaaga agaatttgga catttagagg gtgatacgat
      241 tgtcggcatc caccataaaa gtgccgtcat cacactcgtt gagcgacttt caaaagccat
      301 tatcgccttg aaaccagaag gccgtaaggc agttgatatt gaaaattcga ttaatgaatg
      361 gcttcaatcc gtacccaaaa atcttttcaa atcaattacc tttgattgtg gaaaagaatt
      421 ctctaattgg aaaagtatta gtaatacgaa tgatattgat atttatttcg cagacccagg
      481 aacgccttcc caacggggat taaatgaaca ttcaaacgga ctcctccgaa aaga
//
LOCUS       DACDHJ010000422          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.427, whole genome shotgun sequence.
ACCESSION   DACDHJ010000422 DACDHJ010000000
VERSION     DACDHJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.427"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..>509
                     /locus_tag="IV223_002753"
     CDS             13..>509
                     /locus_tag="IV223_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8712975.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLA"
BASE COUNT          181 a           77 c          102 g          149 t
ORIGIN      
        1 aggaattatt ttatgtctga ggagttattg cgcgctgcac gtaatggtga cgaagaagca
       61 tttgctgccc tgtatcaaaa atattttcca cttttactac gtttgaaaaa cacctattat
      121 ttaagaaatt atgtttctga ggattgggaa caagaagcaa ggatcgcttt atatcaggca
      181 ttgagaactt acgaaaaaag caaaggtgtt agctttggaa gttattataa aagcgttata
      241 agcaatcaaa tctattctct gttgaggaag cagaacgcgc tgaagcgtcg tgatcaaaaa
      301 gatgaagtct caatcgacca aaaaatagag acagaaggac cagacttttt agcagagatc
      361 acattgcaag aacctttagc aattcagcat cttttattaa aagaagcaat cgaaaattgc
      421 gaaatccagt tttcaaaaat agaagctaaa atttttcatt attatatgca aggcgttgat
      481 tttgtagaca tggctgaatg tttagcaat
//
LOCUS       DACDHJ010000423          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.428, whole genome shotgun sequence.
ACCESSION   DACDHJ010000423 DACDHJ010000000
VERSION     DACDHJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.428"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            28..474
                     /locus_tag="IV223_002754"
     CDS             28..474
                     /locus_tag="IV223_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8712976.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT          216 a           72 c          103 g          105 t
ORIGIN      
        1 tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt tcctgcatca
       61 atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa tgcattatta
      121 gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc aaaaaaatgg
      181 tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa tccaagcaag
      241 acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga tgtgatcgta
      301 gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc aagcaaccat
      361 tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa caaagaatca
      421 actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta ttgaacatat
      481 gtaaaggggt tgtgac
//
LOCUS       DACDHJ010000424          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.429, whole genome shotgun sequence.
ACCESSION   DACDHJ010000424 DACDHJ010000000
VERSION     DACDHJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.429"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..>496
                     /locus_tag="IV223_002755"
     CDS             290..>496
                     /locus_tag="IV223_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP8712977.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGS"
BASE COUNT          179 a           55 c           81 g          181 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttct
//
LOCUS       DACDHJ010000425          490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.430, whole genome shotgun sequence.
ACCESSION   DACDHJ010000425 DACDHJ010000000
VERSION     DACDHJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.430"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            215..>490
                     /locus_tag="IV223_002756"
     CDS             215..>490
                     /locus_tag="IV223_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712978.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFF"
BASE COUNT          119 a           74 c           87 g          210 t
ORIGIN      
        1 ttacgactct ggtatctcat tgttttgcta cgaaatcggc ttattagctt cggccatctt
       61 tttcatattt gtgttagaat atttcagaaa aaaataaact tccagtcagt tttggctgga
      121 agttttttat ttggtgcaga tcttttcagc tgttattcat acaagttgat ccatttttct
      181 attattaagt acataccatt gttactcttt gaatgtgggg gagaaaaaaa taaaaggagc
      241 tggtattttg ttagttatga gtaaagcttt gtttttactt ccctgcacgt tcatgtttat
      301 tgttttatgt tatttcaaaa gaagaaaaaa atggtgctgg atcgatgttc tactattcct
      361 tccctttttc ccgcttattt attttcttga agaaatatgg gatgatatgg catgtgattc
      421 tgcttttctt tttagtcatt cattggggct tatgatctct cttacgatct ttgccattgt
      481 gctatttttt
//
LOCUS       DACDHJ010000426          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.431, whole genome shotgun sequence.
ACCESSION   DACDHJ010000426 DACDHJ010000000
VERSION     DACDHJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.431"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>487)
                     /locus_tag="IV223_002757"
     CDS             complement(<1..>487)
                     /locus_tag="IV223_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP8712979.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACDHJ010000427          482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.432, whole genome shotgun sequence.
ACCESSION   DACDHJ010000427 DACDHJ010000000
VERSION     DACDHJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.432"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..431
                     /locus_tag="IV223_002758"
     CDS             18..431
                     /locus_tag="IV223_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8712980.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          177 a           80 c           91 g          134 t
ORIGIN      
        1 tgaaaggatg atcaaaaatg gcaaatgtac ctagcattcg cgacatgttc cctgatttca
       61 atgacgtgtt tagtccagca ttcaatgatt ttttaggtgt ttcaagttat ccaaaagtag
      121 atttagtcga aaatgaaaaa gaatacaaac taactgctga tatgcctggc tgtgacaaag
      181 aagacacgac agttgaatat tcagataata ctttgacgat ctccgccaat catgagtccc
      241 atacagaaga taaagaagat ggcaactatg tacggaaaga aagacattcg gtctcttaca
      301 aacgttcatt ctatcttcct aatgttgatg aagaaaaaat cactggaacc tttaaaaacg
      361 gtgtgctgaa attggtatta cctaaaacag cttatcagcc aaaagaaaca aaaaaaattg
      421 agttaaatta gaacttatcg cctctcgatg aatgaggggc gatttttata tatttggata
      481 ag
//
LOCUS       DACDHJ010000428          477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.433, whole genome shotgun sequence.
ACCESSION   DACDHJ010000428 DACDHJ010000000
VERSION     DACDHJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.433"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>477
                     /locus_tag="IV223_002759"
     CDS             <1..>477
                     /locus_tag="IV223_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8712981.1"
                     /translation="AKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVL
                     ETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRK
                     TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          165 a           85 c           87 g          140 t
ORIGIN      
        1 gccaaagttg gctataactc aacaaaaaaa tcttattttt atggtcttaa aattcatatg
       61 atcgttacta agactggctt tccgattact tactcaatca cgaatccagg tgtccatgac
      121 gtgaaagtgt tagagacttt atcggaagaa gcgaatcttc ccaatatcct aggggacaaa
      181 ggatatatta gccataaaat ccatgaaaag cttgccctta agggcattac tatatccgtt
      241 ccgccacgta aaaacatgga taaatcagaa aaactagacc acagcttact tggaaaacaa
      301 agaaaaacag ttgagactgt tttttcttcc ttagaaaaat taggttgtca aaatttcaac
      361 tcacgttctg ttaaagggct agagagtaga tttgaaagca tattactggc ttacagtgtt
      421 ctattaagtc gagcacaacg acgttttgaa ggaactttga gatattcttt aggatat
//
LOCUS       DACDHJ010000429          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.434, whole genome shotgun sequence.
ACCESSION   DACDHJ010000429 DACDHJ010000000
VERSION     DACDHJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.434"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IV223_002760"
     tRNA            complement(20..91)
                     /locus_tag="IV223_002760"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IV223_002761"
     tRNA            complement(113..200)
                     /locus_tag="IV223_002761"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IV223_002762"
     tRNA            complement(225..298)
                     /locus_tag="IV223_002762"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IV223_002763"
     tRNA            complement(312..382)
                     /locus_tag="IV223_002763"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IV223_002764"
     tRNA            complement(384..459)
                     /locus_tag="IV223_002764"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACDHJ010000430          457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.435, whole genome shotgun sequence.
ACCESSION   DACDHJ010000430 DACDHJ010000000
VERSION     DACDHJ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.435"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(182..445)
                     /locus_tag="IV223_002765"
     CDS             complement(182..445)
                     /locus_tag="IV223_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP8712982.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          172 a           85 c           65 g          135 t
ORIGIN      
        1 aacattagaa aaaatagaat caaaaaaacc caagactagt gccgaaataa gggaaacaat
       61 cgaagctaca aaaaataatg atttcgttga acaggaccaa tttatttgtt tccgatattt
      121 ctcaaaaaga actttctttg ggaaagttaa aggcgactgt aaagtcgcct ttagtccttt
      181 tttaccaagc tgctgcatat ttagctccat attttttaat taatgatttg gctgtagcaa
      241 caataccagc tgtaattaat ccgcctccaa caactgcagc aacaatagaa atgattgtag
      301 caaccgtgct accagtatta attatgtcaa taactttttt agctgcataa gaagaaattc
      361 caatgttagc acttagcatt ggtacaccaa caaagaatcc cgcaaaacct aaaattggta
      421 aaactaataa taaattcttt ttcataataa ttcctct
//
LOCUS       DACDHJ010000431          457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.436, whole genome shotgun sequence.
ACCESSION   DACDHJ010000431 DACDHJ010000000
VERSION     DACDHJ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.436"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..419
                     /locus_tag="IV223_002766"
     CDS             <1..419
                     /locus_tag="IV223_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8712983.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          156 a           92 c           81 g          128 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaactacga attatgattc gtcatggcaa aacattttta accaaataaa
      421 cagaacagaa agaggttttc ttctttctgt tctgtcc
//
LOCUS       DACDHJ010000432          457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.437, whole genome shotgun sequence.
ACCESSION   DACDHJ010000432 DACDHJ010000000
VERSION     DACDHJ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.437"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..387)
                     /locus_tag="IV223_002767"
     CDS             complement(<1..387)
                     /locus_tag="IV223_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712984.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQ"
BASE COUNT          151 a           75 c           63 g          168 t
ORIGIN      
        1 ttggctcgtc tcttctcgca acagattaaa ctgattttca aaactttcaa tgaatcgttc
       61 attcaaccat tcgattgttt catcttcatt ttttctcaag tatttttgat ccaatttatc
      121 atgaagataa agccaagaga gtgtcttatt gataaaaata acggattttt ctcctaatac
      181 tccttttaag tggtaaaatc ctatatccga aaaaaatagc gactgattgc tagcaatatc
      241 taaagaatca gcttgttcag ccaacaggtt gattgctaaa aagtggaata atttcttttt
      301 ttcataagca gacagaggat aatactgttt taaatctttt aacatttttt ctgtttttat
      361 aattataggt agcgtataca attccacaaa acttatctct gttgagttaa aaagattata
      421 taaaaaaagc cgaatgtttt taggattccc tgtcaac
//
LOCUS       DACDHJ010000433          450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.438, whole genome shotgun sequence.
ACCESSION   DACDHJ010000433 DACDHJ010000000
VERSION     DACDHJ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.438"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          143 a           90 c           67 g          150 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat
//
LOCUS       DACDHJ010000434          450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.439, whole genome shotgun sequence.
ACCESSION   DACDHJ010000434 DACDHJ010000000
VERSION     DACDHJ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.439"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>450)
                     /locus_tag="IV223_002768"
     CDS             complement(<1..>450)
                     /locus_tag="IV223_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712985.1"
                     /translation="TGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLLENLD
                     LYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDDSKES
                     VTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDED"
BASE COUNT          154 a           87 c           50 g          159 t
ORIGIN      
        1 catcttcatc atctaatttt tttatcaatc ctataaaagt cataagtgat agtaacttat
       61 tacatttatc tatgtcataa gttttaccat agtaagaact cattttttta gctaattgtt
      121 cccccgacaa aatacaagtc acactttcct tactatcatc aaaaaaatta acatcagaaa
      181 gcaagacatc atagacacgt cgcatttcct ctagataatt ccttattgtc ctgaaaagtt
      241 tgtctttcat tgattcaaga gtgtcaaaaa gtacttttga ttcctctttc aattttcgaa
      301 aatgtgtagg atacaagtct aggttctcta atagccaatt aaatgtttct acccgactgt
      361 ctttctttaa aattagcatc acaactccaa taaaattttc actgatatca caattactgg
      421 aatgacaata aaaaatggaa tgcccagttt
//
LOCUS       DACDHJ010000435          449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.440, whole genome shotgun sequence.
ACCESSION   DACDHJ010000435 DACDHJ010000000
VERSION     DACDHJ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.440"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>449
                     /locus_tag="IV223_002769"
     rRNA            <1..>449
                     /locus_tag="IV223_002769"
                     /product="23S ribosomal RNA"
BASE COUNT          136 a           99 c          132 g           82 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggca
//
LOCUS       DACDHJ010000436          447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.441, whole genome shotgun sequence.
ACCESSION   DACDHJ010000436 DACDHJ010000000
VERSION     DACDHJ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.441"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          160 a           75 c           69 g          143 t
ORIGIN      
        1 aagactatgt ctgttccata tacaaatatg aaaatcagta aactccattt aacttttttc
       61 gtagtgtgtc tttttataca tgtttatcgt tattaatagt gaataggaaa aaaactattt
      121 ttgatcattg acaattaaat acaatttttg aataacgatc cctgctattc gagctagaaa
      181 ctcaagtacg ccatgacctg attagcgaca aatacttaga gatttctttt tgttctggga
      241 aaacaaaatg ccatgacaat agcagcaaat aatgatactt ccgtaaggct cgccgtaaac
      301 tggggagagg tgaaattcct atgtgcttac ccaaagcaat tcaaaaattt cataactact
      361 attttaggag atgattttat agaatccaga gtctacctga ataaaaaaca aacctgtgca
      421 tttctaggaa tcgataataa tactcta
//
LOCUS       DACDHJ010000437          445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.442, whole genome shotgun sequence.
ACCESSION   DACDHJ010000437 DACDHJ010000000
VERSION     DACDHJ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.442"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IV223_002770"
     CDS             <1..435
                     /locus_tag="IV223_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8712986.1"
                     /translation="EKDRLQDQLLLDNFETIMTNDSIATFIPERVLNLIKTFSSSYSE
                     AQKTVQTIHNAKKKAETESGISIIFEELDSYYVNAEQELYTTLLKAYQKLKTEKVENI
                     QNLIFVYVKNWFIEKPIAAKVSSEKRLNSESFPKTITKDWLE"
BASE COUNT          184 a           70 c           69 g          122 t
ORIGIN      
        1 gaaaaagacc gcctacaaga tcaattgttg ttagacaatt ttgagacaat tatgacaaac
       61 gacagcattg ctacgtttat ccctgaacga gtattaaatt taataaaaac attttcttca
      121 agttacagtg aagctcaaaa aactgtccag actattcata atgcaaagaa aaaagccgaa
      181 acagaaagtg gtatttcaat aatttttgaa gaactcgata gttattatgt caatgcagaa
      241 caagaattat acacgacact gttaaaagcc tatcaaaaat taaaaaccga aaaagttgaa
      301 aatatccaga acctgatttt tgtctatgta aaaaattggt ttatcgaaaa accaatagct
      361 gctaaagtat caagtgaaaa acgtttgaat tctgaaagct tcccaaaaac tattacgaaa
      421 gactggttag agtgaaattt cactg
//
LOCUS       DACDHJ010000438          442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.443, whole genome shotgun sequence.
ACCESSION   DACDHJ010000438 DACDHJ010000000
VERSION     DACDHJ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.443"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..228
                     /locus_tag="IV223_002771"
     CDS             37..228
                     /locus_tag="IV223_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8712987.1"
                     /translation="MVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEA
                     EYYEKDEWEDLTPEGREIL"
     gene            228..>442
                     /locus_tag="IV223_002772"
     CDS             228..>442
                     /locus_tag="IV223_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8712988.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELD"
BASE COUNT          158 a           66 c           89 g          129 t
ORIGIN      
        1 cttgttacaa aatctagatt acaaggaagt tctgtagtgg taactttacc ttctgataac
       61 ggaaagaaac cttcagaaaa ccaagaatat atcgttgtgt attctgatga tggaacaatt
      121 acgttagttc caaaaattga agatccattt agtggtgggg aagaggccga gtattatgag
      181 aaagacgaat gggaagattt aactcctgaa gggagagaaa ttttatagtg tcagaagaaa
      241 gaatttatat tcctaaaaaa ggtgatattg tatggattga ttttgatccc tccgtaggaa
      301 aagaaattca aaaaagacga ccaggactag tcgtctctcg ttatgaattt aaccgtaaaa
      361 caatgttttc ggtcatatgt ccaattactt ccactataaa gaatttaccg acacgttact
      421 cattacctga agagctagat at
//
LOCUS       DACDHJ010000439          440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.444, whole genome shotgun sequence.
ACCESSION   DACDHJ010000439 DACDHJ010000000
VERSION     DACDHJ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.444"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..>440
                     /locus_tag="IV223_002773"
     CDS             11..>440
                     /locus_tag="IV223_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8712989.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DACDHJ010000440          438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.445, whole genome shotgun sequence.
ACCESSION   DACDHJ010000440 DACDHJ010000000
VERSION     DACDHJ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.445"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..247
                     /locus_tag="IV223_002774"
     CDS             <1..247
                     /locus_tag="IV223_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712990.1"
                     /translation="VKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPAAKTRMKK
                     DVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          138 a           72 c           68 g          160 t
ORIGIN      
        1 tgtcaaagat ttttataagg tgtgggattc agaagttttc tttttagcaa tcgaagatag
       61 cttattaatt aatctctact attctaataa gaactacttt aaaatccctg ctgcgaaaac
      121 tagaatgaag aaagacgtat attttttgtt tgatatcgtg actgatgtgc cggacgcacg
      181 tagcgatcat cggcgttatg actatataaa gtatactttc gttgatccag aaagatacaa
      241 agattaaata aaaaaataac aaacaaactc gcttgaaatt cattaggtga gtttgtttat
      301 tattttttct gcttctcgat tcatcagcgc catatctgtt ttcgtaatgg tttcattagg
      361 aatatagtca gcacattttc ttttctctaa cattctatac ccactattat gtattcctaa
      421 ggtattcaat tccttaac
//
LOCUS       DACDHJ010000441          435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.446, whole genome shotgun sequence.
ACCESSION   DACDHJ010000441 DACDHJ010000000
VERSION     DACDHJ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.446"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..389
                     /locus_tag="IV223_002775"
     CDS             273..389
                     /locus_tag="IV223_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8712991.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          167 a           67 c           83 g          118 t
ORIGIN      
        1 gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa
       61 agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc
      121 cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt
      181 aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg
      241 actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat
      301 accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag
      361 atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg
      421 aaaaacgttt ttcgg
//
LOCUS       DACDHJ010000442          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.447, whole genome shotgun sequence.
ACCESSION   DACDHJ010000442 DACDHJ010000000
VERSION     DACDHJ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.447"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          151 a           71 c           58 g          152 t
ORIGIN      
        1 gtattatctg tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg
       61 atcactttac cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa
      121 cgtaactaca taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga
      181 gcaatacggt tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc
      241 tctaacgttc agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt
      301 ctatttgtcg aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa
      361 aaagcagtta ctattgctag aataacatca aggaataata agttttggtt ttcagataat
      421 agtcgataag tt
//
LOCUS       DACDHJ010000443          430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.448, whole genome shotgun sequence.
ACCESSION   DACDHJ010000443 DACDHJ010000000
VERSION     DACDHJ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.448"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..419
                     /locus_tag="IV223_002776"
     CDS             <1..419
                     /locus_tag="IV223_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8712992.1"
                     /translation="KKYAGKPIIIVNTATKCGLAPQFKQLEELYQAYQTQGLVILGFP
                     SNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYLKNEAPGTVTKAI
                     KWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          157 a           84 c           66 g          123 t
ORIGIN      
        1 tgaaaaaata tgcaggcaaa ccgataatca ttgttaacac tgcaacaaaa tgcggtctag
       61 cacctcaatt taaacaatta gaagaacttt accaagctta tcagacacaa ggtctggtta
      121 tcttaggctt tccttccaat caatttaaac aagagctaga ttcagcgact gaagcagcag
      181 aagcttgtaa aaccacttat ggcgttacct tccctatgca tcaattgatt gacgtaaatg
      241 gagaaaatac agatcctctt tttacttatc ttaaaaatga agctcctggt actgtcacta
      301 aagcaataaa atggaatttt actaaatttt tgattgatag aaatggcaaa gtaatcgaac
      361 gatttgcacc acaaacttct ccattgaaaa tgaccgctgc aattgaatcc gttctttaac
      421 ttaataaaaa
//
LOCUS       DACDHJ010000444          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.449, whole genome shotgun sequence.
ACCESSION   DACDHJ010000444 DACDHJ010000000
VERSION     DACDHJ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.449"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>428)
                     /locus_tag="IV223_002777"
     CDS             complement(<1..>428)
                     /locus_tag="IV223_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8712993.1"
                     /translation="SIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDR
                     KVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETD
                     FYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVS"
BASE COUNT          137 a           81 c           56 g          154 t
ORIGIN      
        1 tgatacaagt acttgaagaa atccattatg catgtttcca tgtgctcttt ttagttcatt
       61 cacatctgta gtatcaagat ctactttttt caaaaatttt gcatcgctat tcccatagaa
      121 atcggtctct ccataaccaa ataacaaagt tggtcttata actttaagcg cagctttcca
      181 aatcgtaaaa cgaccagctg aaatttcgga gttatcacta gaatgctgta atattacttc
      241 tttttctgga agtggagttt tatctgattc atttgactct ataacttcta cttttctatc
      301 aatcgataca ggaattgttg ctaaaaaatc cttagcataa ttagtaccaa aagaaaaata
      361 taccaataga actaataata tgcttaccat ccatttgatt tttacaggta gtacttctct
      421 aatgctct
//
LOCUS       DACDHJ010000445          424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.450, whole genome shotgun sequence.
ACCESSION   DACDHJ010000445 DACDHJ010000000
VERSION     DACDHJ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.450"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            60..>424
                     /locus_tag="IV223_002778"
     CDS             60..>424
                     /locus_tag="IV223_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712994.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREAL"
BASE COUNT          114 a           77 c           65 g          168 t
ORIGIN      
        1 cacaaacaaa tatcgttatt ttaaaatgag ggcgaactca cacaattagg aggaatagta
       61 tgccatttat tgtttacttt tttctttcat tactcgtgat ctatattcca ttaccaacat
      121 ttttcctgct cgtcaatcgt ctagcagagc aacatgatac gattgctgct cttacgaaac
      181 tttgtttact tctatttgtt tgtctagatt actttacgag ctattattgg ctaaaacaga
      241 caagagtaaa aaagagatat tatttcttcc ttgtcttttt caatctcttt gagtttctga
      301 tccattgctt tttacttcat cagtatcata cgatccattt gtggatcatc tgggttttca
      361 aagtggtcgt attattttgt atgttcacct ttcctatctc aagaaaattt agggaggcat
      421 tgtt
//
LOCUS       DACDHJ010000446          419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.451, whole genome shotgun sequence.
ACCESSION   DACDHJ010000446 DACDHJ010000000
VERSION     DACDHJ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.451"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          121 a           68 c           75 g          155 t
ORIGIN      
        1 cacaatcgta ggttttaaat tcataatcgt tagatatttt agttattttt cactcaaaaa
       61 aatagggaac tagtgtttgc atatgtgctt ttttgagtta taataattca tgaagagagg
      121 taattcactt atttccccaa gctgaaaagc ctgatattac ttcttttcag gcagtgctgc
      181 taatattcaa ttacactttt ttgtatattt ttagttgttt taaatttaat tgtctgctat
      241 gcttgtgatg aattgatttt cgttctgcac ttttggacga taaatttgga caattgaaaa
      301 tacaaaaaaa agaaaggccc tgtgagactc ggaaggctgg taaccttcgt cgacgtccac
      361 aggtggcatt aagatcactc ccttaatgcg tttacctttc agtgttacgg tattacccg
//
LOCUS       DACDHJ010000447          416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.452, whole genome shotgun sequence.
ACCESSION   DACDHJ010000447 DACDHJ010000000
VERSION     DACDHJ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.452"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(255..>416)
                     /locus_tag="IV223_002779"
     CDS             complement(255..>416)
                     /locus_tag="IV223_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8712995.1"
                     /translation="KRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTE
                     IKVLMGITA"
BASE COUNT          130 a           74 c           70 g          142 t
ORIGIN      
        1 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
       61 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
      121 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
      181 ggtaatcaag gaaatatgaa ggttctgttg cgaagtttga aaatcaaacg cgccctctct
      241 gaactccaaa tatcttaggc tgttattccc attaatacct tgatttcagt agacaccgaa
      301 aagccgaaga gcgttccatt tcttcggttc tttttatata ttcctcgaat ggtctccatg
      361 cccttaatcg tggaagaggc tgtacggaga ctttgataaa atttatttcg tcgttt
//
LOCUS       DACDHJ010000448          413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.453, whole genome shotgun sequence.
ACCESSION   DACDHJ010000448 DACDHJ010000000
VERSION     DACDHJ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.453"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..228
                     /locus_tag="IV223_002780"
     CDS             <1..228
                     /locus_tag="IV223_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8712996.1"
                     /translation="RPGEKLYEELMTTDEIERSYENDKMYIIPPHYHATDELYKDYEK
                     IKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            240..>413
                     /locus_tag="IV223_002781"
     CDS             240..>413
                     /locus_tag="IV223_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8712997.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQ"
BASE COUNT          170 a           56 c           79 g          108 t
ORIGIN      
        1 aggccaggag aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat
       61 gaaaatgata aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa
      121 gattatgaaa aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga
      181 caggaattta ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga
      241 tgaaatcaga agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt
      301 tagctttaga aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa
      361 catttaaaac gaataagcta gtttccaaaa aggctccaat ggcaaaatat cag
//
LOCUS       DACDHJ010000449          410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.454, whole genome shotgun sequence.
ACCESSION   DACDHJ010000449 DACDHJ010000000
VERSION     DACDHJ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.454"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          167 a           58 c           70 g          115 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata
//
LOCUS       DACDHJ010000450          408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.455, whole genome shotgun sequence.
ACCESSION   DACDHJ010000450 DACDHJ010000000
VERSION     DACDHJ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.455"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>408
                     /locus_tag="IV223_002782"
     CDS             <1..>408
                     /locus_tag="IV223_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8712998.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGR"
BASE COUNT          128 a           76 c           88 g          116 t
ORIGIN      
        1 caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca
       61 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
      121 tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga
      181 cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac
      241 tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg
      301 tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt
      361 tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagaca
//
LOCUS       DACDHJ010000451          402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.456, whole genome shotgun sequence.
ACCESSION   DACDHJ010000451 DACDHJ010000000
VERSION     DACDHJ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.456"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..266
                     /locus_tag="IV223_002783"
     CDS             <1..266
                     /locus_tag="IV223_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8712999.1"
                     /translation="GTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIH
                     FYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          140 a           56 c           70 g          136 t
ORIGIN      
        1 ctggaaccaa atatcaagat attgttgaaa aaattacatc atttgtgacg aaaaagacag
       61 tctgttgtct agataagtta aagactatcg gaatgatcaa tcaaaattta gtctttaatg
      121 aagaagaaat ccacttctac atttactcat tctacgctac attctatgat atattgaagc
      181 atagctattc aaaagaaaag actatgctat taaccagaag attgtatcaa ttttctgttc
      241 ctgggtggat tgaattactg agttaattaa aaaaagcttg cattgagaat tgttctcagt
      301 tatgcttttt gataagatcg cactgttatc tgaataacgg tgcattttta tcagcttaag
      361 ataacagtgc gatcttattt tttggaggtg aaagagtgtc aa
//
LOCUS       DACDHJ010000452          392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.457, whole genome shotgun sequence.
ACCESSION   DACDHJ010000452 DACDHJ010000000
VERSION     DACDHJ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.457"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..>392
                     /locus_tag="IV223_002784"
     CDS             3..>392
                     /locus_tag="IV223_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8713000.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASD"
BASE COUNT          139 a           62 c           77 g          114 t
ORIGIN      
        1 aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta aaggaaaaga
       61 caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta atggctggtc
      121 aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct aacgaattta
      181 actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt tcccattctg
      241 gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga gcaatgacta
      301 tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat cctgttcact
      361 atgattgggg taaagatgtt gatgctagtg at
//
LOCUS       DACDHJ010000453          388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.458, whole genome shotgun sequence.
ACCESSION   DACDHJ010000453 DACDHJ010000000
VERSION     DACDHJ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.458"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          117 a           62 c           69 g          140 t
ORIGIN      
        1 taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt ttgtttaggt
       61 ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag acgtttgtcg
      121 cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt cgccatttgg
      181 aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg attgtaatac
      241 caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta gaagttgtat
      301 caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc atctcctttg
      361 taatgtaatt tagttcttga aagtttcc
//
LOCUS       DACDHJ010000454          384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.459, whole genome shotgun sequence.
ACCESSION   DACDHJ010000454 DACDHJ010000000
VERSION     DACDHJ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.459"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          132 a           53 c           65 g          134 t
ORIGIN      
        1 aggagagtat ttgatactaa ctagtaggaa aaaatgagat aaaaagtaaa tggaatcatt
       61 taaggtaatc tgaaataaaa aatatacagc cctgtggtac tttacctaca aggctgtata
      121 tattttttac tctcagcgtt tttgactccg tgtctgactc cgttttattg attttgaatg
      181 ttattcaatg accatttaac aggtcaaatt tgatgttttt gattccgtat gacgccgttt
      241 gatatctagt gatatagtga tttgagtcta catcgaataa gggaataaac caataaaaaa
      301 tgatttagaa tattaaaata acaaaaaaga ggactacctg tattagtcct cttttttgac
      361 cagattttga ccagtctatt ttac
//
LOCUS       DACDHJ010000455          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.460, whole genome shotgun sequence.
ACCESSION   DACDHJ010000455 DACDHJ010000000
VERSION     DACDHJ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.460"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IV223_002785"
     tRNA            complement(21..94)
                     /locus_tag="IV223_002785"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IV223_002786"
     tRNA            complement(108..181)
                     /locus_tag="IV223_002786"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IV223_002787"
     tRNA            complement(209..281)
                     /locus_tag="IV223_002787"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IV223_002788"
     tRNA            complement(301..374)
                     /locus_tag="IV223_002788"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACDHJ010000456          379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.461, whole genome shotgun sequence.
ACCESSION   DACDHJ010000456 DACDHJ010000000
VERSION     DACDHJ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.461"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>379
                     /locus_tag="IV223_002789"
     CDS             <1..>379
                     /locus_tag="IV223_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8713001.1"
                     /translation="PEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQH
                     LLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEK
                     ISDETETFYLDTTHNTVLFGKLIK"
BASE COUNT          150 a           70 c           58 g          101 t
ORIGIN      
        1 tccagaagcc acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa
       61 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
      121 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
      181 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
      241 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
      301 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
      361 atttggcaag ttaataaaa
//
LOCUS       DACDHJ010000457          371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.462, whole genome shotgun sequence.
ACCESSION   DACDHJ010000457 DACDHJ010000000
VERSION     DACDHJ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.462"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..>371)
                     /locus_tag="IV223_002790"
     CDS             complement(37..>371)
                     /locus_tag="IV223_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713002.1"
                     /translation="ISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFP
                     DVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFP
                     EEIIDRIE"
BASE COUNT          103 a           76 c           61 g          131 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc a
//
LOCUS       DACDHJ010000458          368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.463, whole genome shotgun sequence.
ACCESSION   DACDHJ010000458 DACDHJ010000000
VERSION     DACDHJ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.463"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            170..>368
                     /locus_tag="IV223_002791"
     CDS             170..>368
                     /locus_tag="IV223_002791"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8713003.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDR"
BASE COUNT          116 a           71 c           64 g          117 t
ORIGIN      
        1 cgtctaatat tcaaattata ctcgatatgc tttagctaat cagccaactc tgtttgtcta
       61 ctatcattat atcaatagac aatatctgct cccgtcttta tgatcgtgcc aaagcaatca
      121 aaagaaaatt gcgaatcact tagatcgaaa gtttactcaa acaaaagcat tgtcagcggt
      181 tgtcacggat ttaacttatg ttcgggtcgg gacaaattgg gcttatattt gtttgatgat
      241 tgatttattc aatcgtgaaa ttattggcca ttcttgcgga aataaaaaag atgcgcagct
      301 tgtaaaaagg gccattcaaa gcattcctta ctcacttcaa gaaatcgaac tcttccatac
      361 ggatcgtg
//
LOCUS       DACDHJ010000459          363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.464, whole genome shotgun sequence.
ACCESSION   DACDHJ010000459 DACDHJ010000000
VERSION     DACDHJ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.464"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..223
                     /locus_tag="IV223_002792"
     CDS             29..223
                     /locus_tag="IV223_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713004.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          161 a           46 c           68 g           88 t
ORIGIN      
        1 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
       61 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      121 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      181 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      241 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      301 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      361 gta
//
LOCUS       DACDHJ010000460          353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.465, whole genome shotgun sequence.
ACCESSION   DACDHJ010000460 DACDHJ010000000
VERSION     DACDHJ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.465"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..353)
                     /locus_tag="IV223_002793"
     CDS             complement(<1..353)
                     /locus_tag="IV223_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713005.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFF"
BASE COUNT          121 a           65 c           66 g          101 t
ORIGIN      
        1 taaaaaaacg gcgaatgaag atgtgttttc atatgaccaa aatataataa ttctgaaatt
       61 tctaaaggtg tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca cttcaattca
      121 tcgaatccac gcctgttacg agaagaggaa aagaaacgct gaatattttc tgtaccacgg
      181 attactttca gccctgtatg ggattcatat tctccagcag aagcagatgg atcaagcaag
      241 agcaagtttt gaggaaaatg cacgatactg tttgcaaagt cagtagcagt cagccctttt
      301 gttaaaacag cattacttgt ggtatcaata tgtacataaa ctgactcagt cat
//
LOCUS       DACDHJ010000461          347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.466, whole genome shotgun sequence.
ACCESSION   DACDHJ010000461 DACDHJ010000000
VERSION     DACDHJ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.466"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..>347
                     /locus_tag="IV223_002794"
     CDS             89..>347
                     /locus_tag="IV223_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713006.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGV"
BASE COUNT          126 a           55 c           58 g          108 t
ORIGIN      
        1 tactgactta tactcttact tcaagaggaa aatgaaaaaa aattattttt ggctgtttct
       61 ttttatttat caaataagga gtggaaaaat gcgagcagca gacttattga caattcttac
      121 aaaatcaccc aaaaaaacag agtatcaagg agtttttttg gctcaagaac aaaaactggt
      181 gcctgtgaca atggtccaga taaataaagc aaatcaattt atctttttat ttgaacacgg
      241 caaagcacca cttgctatga aagacatctt tctttttttg atgaagaacc gtgagaaagc
      301 tgtttattat cagtcaaaag aagaactcat tcctctttat ggagtga
//
LOCUS       DACDHJ010000462          345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.467, whole genome shotgun sequence.
ACCESSION   DACDHJ010000462 DACDHJ010000000
VERSION     DACDHJ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.467"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>345
                     /locus_tag="IV223_002795"
     CDS             <1..>345
                     /locus_tag="IV223_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8713007.1"
                     /translation="AEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSS
                     YTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLF
                     NREILGYSCGEH"
BASE COUNT          129 a           60 c           66 g           90 t
ORIGIN      
        1 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
       61 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      121 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      181 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      241 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      301 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataa
//
LOCUS       DACDHJ010000463          341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.468, whole genome shotgun sequence.
ACCESSION   DACDHJ010000463 DACDHJ010000000
VERSION     DACDHJ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.468"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>341
                     /locus_tag="IV223_002796"
     CDS             <1..>341
                     /locus_tag="IV223_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713008.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DACDHJ010000464          336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.469, whole genome shotgun sequence.
ACCESSION   DACDHJ010000464 DACDHJ010000000
VERSION     DACDHJ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.469"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..>336
                     /locus_tag="IV223_002797"
     CDS             69..>336
                     /locus_tag="IV223_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8713009.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIE"
BASE COUNT          129 a           43 c           70 g           94 t
ORIGIN      
        1 caggcaaaga aaactggaat gatttatatc atgcaaacga agtattgaaa atagctgagg
       61 agaaataaat ggaaatttta tttactattt gtggtagagc tgggtctaag gggtttaaaa
      121 ataaaaattt gaaatatttg gcaggaaagc ccctagtcca tttcgcttta gcaatgatag
      181 atatctatca acaaaaacat cctgaacata attgcacgac ttgtttgaat acggacagta
      241 atgagttagt agaattgacg actaagttaa atccgtctgt gaaaattgta gaacgtaaac
      301 ctgaattagc tacagatact gtagggaaaa ttgaag
//
LOCUS       DACDHJ010000465          331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.470, whole genome shotgun sequence.
ACCESSION   DACDHJ010000465 DACDHJ010000000
VERSION     DACDHJ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.470"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..275
                     /locus_tag="IV223_002798"
     CDS             <1..275
                     /locus_tag="IV223_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8713010.1"
                     /translation="MVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYYPKVINNLER
                     SLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          124 a           48 c           65 g           94 t
ORIGIN      
        1 acatggtact aaaattatat gatctcgttt taaatccaga gatcaaagaa aaggaacgta
       61 tacttttgat agacgcaaag acaggcttag aaaaaggtca gtattatcct aaagtcataa
      121 ataatttgga acgtagttta cgtccgttag caatcagggg agaattgtct aaaccagtat
      181 caccgtttta tatggagata tcaacaatag gaaaatttga aaaagaatta ggtcgaggaa
      241 tggcttcggc accgattact tttgggcatt tataaaaacg agttattcat ctcaatgaat
      301 taagaagtat agtcaatctc ataatgcagg c
//
LOCUS       DACDHJ010000466          330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.471, whole genome shotgun sequence.
ACCESSION   DACDHJ010000466 DACDHJ010000000
VERSION     DACDHJ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.471"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(92..292)
                     /locus_tag="IV223_002799"
     CDS             complement(92..292)
                     /locus_tag="IV223_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713011.1"
                     /translation="MAKAKTPFEKAASKILERRGVSETQWKQEVLEKAQFDLLRGKDK
                     DLEGYIYQREREKLVFDEIIKH"
BASE COUNT           91 a           67 c           52 g          120 t
ORIGIN      
        1 aatgctctaa aaatatcaac cgcagctact actttcggtt gtgttaaaaa attcattgta
       61 attgattcaa acataaaatt gttcgctcct tttagtgttt aataatttca tcgaaaacaa
      121 gcttttcacg ttcacgttga tagatatatc cttctaagtc cttgtctttt ccccgtaata
      181 ggtcaaattg tgctttttca aggacttctt gtttccactg tgtttcagat acaccgcggc
      241 gttctaagat tttagacgcg gctttttcaa atggcgtttt agcttttgcc actgtgattc
      301 ctccttcagg acaaataaaa agactagcaa
//
LOCUS       DACDHJ010000467          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.472, whole genome shotgun sequence.
ACCESSION   DACDHJ010000467 DACDHJ010000000
VERSION     DACDHJ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.472"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>321
                     /locus_tag="IV223_002800"
     CDS             <1..>321
                     /locus_tag="IV223_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8713012.1"
                     /translation="KNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFM
                     IQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLR
                     NIFT"
BASE COUNT          117 a           62 c           60 g           82 t
ORIGIN      
        1 aaaaaaatta tccttactta atgaccgatg tactctatat aaaagtacga gaagaaaatc
       61 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
      121 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      181 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      241 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      301 tcctaagaaa tatctttacc a
//
LOCUS       DACDHJ010000468          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.denovo.473, whole genome shotgun sequence.
ACCESSION   DACDHJ010000468 DACDHJ010000000
VERSION     DACDHJ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.denovo.473"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IV223_002801"
     CDS             complement(<1..>321)
                     /locus_tag="IV223_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8713013.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACDHJ010000469        20985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.guided.030, whole genome shotgun sequence.
ACCESSION   DACDHJ010000469 DACDHJ010000000
VERSION     DACDHJ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.guided.030"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(134..577)
                     /locus_tag="IV223_002802"
     CDS             complement(134..577)
                     /locus_tag="IV223_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8713014.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IV223_002803"
     CDS             complement(795..2135)
                     /locus_tag="IV223_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8713015.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IV223_002804"
     CDS             2404..3300
                     /locus_tag="IV223_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8713016.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTSFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IV223_002805"
     CDS             complement(3450..4391)
                     /locus_tag="IV223_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8713017.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IV223_002806"
     CDS             4517..5638
                     /locus_tag="IV223_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8713018.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IV223_002807"
     CDS             5896..7398
                     /locus_tag="IV223_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8713019.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IV223_002808"
     CDS             7465..7992
                     /locus_tag="IV223_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8713020.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IV223_002809"
     CDS             8203..9264
                     /locus_tag="IV223_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8713021.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IV223_002810"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IV223_002810"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8713022.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IV223_002811"
     CDS             10177..12159
                     /locus_tag="IV223_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8713023.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IV223_002812"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IV223_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8713024.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IV223_002813"
     CDS             12881..14305
                     /locus_tag="IV223_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8713025.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IV223_002814"
     CDS             complement(14486..15259)
                     /locus_tag="IV223_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8713026.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IV223_002815"
     CDS             complement(15256..16161)
                     /locus_tag="IV223_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8713027.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IV223_002816"
     CDS             complement(16315..16635)
                     /locus_tag="IV223_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8713028.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IV223_002817"
     CDS             17109..17795
                     /locus_tag="IV223_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8713029.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IV223_002818"
     CDS             17797..18741
                     /locus_tag="IV223_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8713030.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IV223_002819"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IV223_002819"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8713031.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IV223_002820"
     CDS             19663..20940
                     /locus_tag="IV223_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713032.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacatc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaacga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACDHJ010000470        13213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.guided.055, whole genome shotgun sequence.
ACCESSION   DACDHJ010000470 DACDHJ010000000
VERSION     DACDHJ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.guided.055"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(204..2162)
                     /locus_tag="IV223_002821"
     CDS             complement(204..2162)
                     /locus_tag="IV223_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8713033.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="IV223_002822"
     CDS             complement(2159..3031)
                     /locus_tag="IV223_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8713034.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="IV223_002823"
     CDS             complement(3373..4245)
                     /locus_tag="IV223_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8713035.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="IV223_002824"
     CDS             4373..4942
                     /locus_tag="IV223_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8713036.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="IV223_002825"
     CDS             5009..5746
                     /locus_tag="IV223_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8713037.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="IV223_002826"
     CDS             5977..7245
                     /locus_tag="IV223_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8713038.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="IV223_002827"
     CDS             7732..8196
                     /locus_tag="IV223_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8713039.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="IV223_002828"
     CDS             8180..8809
                     /locus_tag="IV223_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8713040.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr"
                     /locus_tag="IV223_002829"
     CDS             9163..10641
                     /gene="msr"
                     /locus_tag="IV223_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8713041.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="IV223_002830"
     CDS             10688..11134
                     /locus_tag="IV223_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8713042.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="IV223_002831"
     CDS             complement(11333..11848)
                     /locus_tag="IV223_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8713043.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="IV223_002832"
     CDS             11965..13167
                     /locus_tag="IV223_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8713044.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DACDHJ010000471         5299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.guided.155, whole genome shotgun sequence.
ACCESSION   DACDHJ010000471 DACDHJ010000000
VERSION     DACDHJ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.guided.155"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..1991
                     /locus_tag="IV223_002833"
     CDS             105..1991
                     /locus_tag="IV223_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8713045.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2003..2950
                     /locus_tag="IV223_002834"
     CDS             2003..2950
                     /locus_tag="IV223_002834"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8713046.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2970..3497
                     /locus_tag="IV223_002835"
     CDS             2970..3497
                     /locus_tag="IV223_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8713047.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3560..4213)
                     /locus_tag="IV223_002836"
     CDS             complement(3560..4213)
                     /locus_tag="IV223_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8713048.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4345..5187
                     /locus_tag="IV223_002837"
     CDS             4345..5187
                     /locus_tag="IV223_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8713049.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPISIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         1892 a          921 c         1115 g         1371 t
ORIGIN      
        1 atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga
       61 agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa
      121 aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt
      181 tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc
      241 tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc
      301 caactgatta ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg
      361 ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg
      421 aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta
      481 aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta
      541 ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc
      601 agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag
      661 acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac
      721 aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa
      781 accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt
      841 acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc
      901 ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc
      961 agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag
     1021 aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca
     1081 aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc
     1141 aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta
     1201 atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc
     1261 atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt
     1321 acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag
     1381 aattacttga acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt
     1441 caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt
     1501 tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt
     1561 attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca
     1621 aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa
     1681 acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac
     1741 caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag
     1801 aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag
     1861 aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat
     1921 ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg
     1981 tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt
     2041 ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata
     2101 ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt
     2161 tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct
     2221 attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc
     2281 agactataat ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt
     2341 caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc
     2401 ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga
     2461 aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac
     2521 tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc
     2581 tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca
     2641 attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc
     2701 gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac
     2761 attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg
     2821 agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt
     2881 tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat
     2941 cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa
     3001 acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct
     3061 tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg
     3121 gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag
     3181 cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag
     3241 acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta
     3301 cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg
     3361 ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc
     3421 gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag
     3481 gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc
     3541 catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac
     3601 ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa
     3661 agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc
     3721 caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga
     3781 tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa
     3841 tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga
     3901 tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat
     3961 cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg
     4021 ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata tggagggagg
     4081 aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc
     4141 caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt
     4201 tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg
     4261 tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg
     4321 ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt
     4381 acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgat atccatcatg
     4441 atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg
     4501 atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg
     4561 atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc
     4621 ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg
     4681 gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa
     4741 ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac
     4801 aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc
     4861 agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc
     4921 cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt
     4981 aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat
     5041 gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac
     5101 atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg
     5161 tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc
     5221 ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata
     5281 aagactacct ttttgataa
//
LOCUS       DACDHJ010000472         3867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.guided.199, whole genome shotgun sequence.
ACCESSION   DACDHJ010000472 DACDHJ010000000
VERSION     DACDHJ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.guided.199"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..602
                     /gene="dfrG"
                     /locus_tag="IV223_002838"
     CDS             105..602
                     /gene="dfrG"
                     /locus_tag="IV223_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8713050.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(674..2626)
                     /locus_tag="IV223_002839"
     CDS             complement(674..2626)
                     /locus_tag="IV223_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8713051.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2633..2929)
                     /locus_tag="IV223_002840"
     CDS             complement(2633..2929)
                     /locus_tag="IV223_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8713052.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3107..3867
                     /locus_tag="IV223_002841"
                     /pseudo
     CDS             3107..3867
                     /locus_tag="IV223_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1183 a          675 c          639 g         1370 t
ORIGIN      
        1 tagaaaaaat tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa
       61 ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga
      121 ttgctgcgat ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc
      181 ccaaggactg ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga
      241 agaaccttga atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag
      301 ataaggggtt tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt
      361 tatgtaaaaa cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt
      421 tcccttatgt tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt
      481 tttttccaga agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga
      541 atgataaaaa tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt
      601 aacgggtgcg ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc
      661 caagctcttt ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa
      721 tccctaatct ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa
      781 agttgtagta agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag
      841 cgtaaatata gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata
      901 tataacgtct tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca
      961 tctttgcgat ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa
     1021 atcctttatc cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc
     1081 tttctccatc tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta
     1141 gataatgaga tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat
     1201 cgagcagttc tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct
     1261 tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc
     1321 tataaaaggc gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat
     1381 taaccatttg tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta
     1441 aaccatcgac aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc
     1501 tattttcgct atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat
     1561 ttttacgact aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt
     1621 catccattga aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga
     1681 cctcagcaga tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct
     1741 ttgaaccttg tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa
     1801 gccacatctc gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact
     1861 ccaaacaacg cctatacagc cactccagct tatgatagta ggttccaact ccaattccta
     1921 aaatatcaca agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa
     1981 gaatggtgtt cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag
     2041 tgaacttctt acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt
     2101 tgtaaaattg cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat
     2161 gaaattgata atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt
     2221 cttcaacaac agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc
     2281 caataggatt gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca
     2341 ttttatatcg acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat
     2401 tacaacaaaa cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt
     2461 taaaagacgt tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta
     2521 ataaatcata ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat
     2581 ctacaatttc atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca
     2641 ctctatcttt gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc
     2701 attcaataaa cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt
     2761 cagtacgctc tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt
     2821 cccaatccac agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca
     2881 cccctttttg cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa
     2941 ttatttcacg ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca
     3001 acaaacgttg atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat
     3061 ttaggtttac attttcactt tttgtactcc aaaactgaac tacttatttt tatttagttg
     3121 tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg caattgccga
     3181 agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa tcatctctgg
     3241 aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc tagtgtacag
     3301 ccatgtacta tcaacattaa tgagtacagc attaattatc atttttattt ttattcgttt
     3361 ggtttctaaa ggtttcaaga atgcagattt tattgctata tttaaaaaat tatttagtgc
     3421 ggcaggaatg acattggttc tgacgagtgc tttttggtat ccgatgtttg aacaaatgct
     3481 ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat gctcatgcat ctaatgtgtt
     3541 cgatagtctg actgaagcta tgaacaacga cttaactact tactcaatgg gactagttgg
     3601 tctgttatct ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca tagaaaaaaa
     3661 gatttattat ggtacgtgtt tgacttggct agctacaact agtttggtgc catggtatct
     3721 tctgcaaaat acgccagcca aattgttaca atttccttgg cggatcttaa gcttgcaaat
     3781 aatattctct tctctgatat taacgatgat atttttcaaa aatagaagat acaataagac
     3841 aagagaacta ttttatttat ttatgac
//
LOCUS       DACDHJ010000473          718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E62505_2016
            SAMN12640447-rid9888093.guided.403, whole genome shotgun sequence.
ACCESSION   DACDHJ010000473 DACDHJ010000000
VERSION     DACDHJ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640447
            Sequence Read Archive: SRR10040991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742935.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742935.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 21:37:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,787
            Genes (coding)                    :: 2,738
            CDSs (with protein)               :: 2,738
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 26 of 49
            Pseudo Genes (internal stop)      :: 11 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640447-rid9888093.guided.403"
                     /isolate="E62505_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..653
                     /gene="aac(6')"
                     /locus_tag="IV223_002842"
     CDS             105..653
                     /gene="aac(6')"
                     /locus_tag="IV223_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8713053.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          261 a          110 c          164 g          183 t
ORIGIN      
        1 atgaaagaat agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt
       61 ttcaaaagga ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat
      121 ttgaccgtaa taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc
      181 cggaagaata tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa
      241 tcgcggtagc agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat
      301 acggtatcac aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc
      361 aaataggtac tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca
      421 cgatttattt aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt
      481 atgagcatac atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat
      541 tctatgaaaa attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca
      601 aaccggatat ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag
      661 aggttgtgac agaagtaatc agcttcaaga aataagaagg aatttacgaa aattactt
//
